LOCUS       DANLUA010000001        70356 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLUA010000001 DANLUA010000000
VERSION     DANLUA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 70356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70356
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            224..1582
                     /locus_tag="PXB44_000001"
     CDS             224..1582
                     /locus_tag="PXB44_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL5280531.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            1585..2820
                     /locus_tag="PXB44_000002"
     CDS             1585..2820
                     /locus_tag="PXB44_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL5280532.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            2813..3586
                     /locus_tag="PXB44_000003"
     CDS             2813..3586
                     /locus_tag="PXB44_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL5280533.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            3586..4212
                     /locus_tag="PXB44_000004"
     CDS             3586..4212
                     /locus_tag="PXB44_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL5280534.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            4214..4810
                     /gene="nqrE"
                     /locus_tag="PXB44_000005"
     CDS             4214..4810
                     /gene="nqrE"
                     /locus_tag="PXB44_000005"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL5280535.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            4821..6050
                     /gene="nqrF"
                     /locus_tag="PXB44_000006"
     CDS             4821..6050
                     /gene="nqrF"
                     /locus_tag="PXB44_000006"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL5280536.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            6162..6596
                     /locus_tag="PXB44_000007"
     CDS             6162..6596
                     /locus_tag="PXB44_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5280537.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            6589..6882
                     /locus_tag="PXB44_000008"
     CDS             6589..6882
                     /locus_tag="PXB44_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5280538.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            6931..7965
                     /locus_tag="PXB44_000009"
     CDS             6931..7965
                     /locus_tag="PXB44_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL5280539.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            7978..8223
                     /gene="nqrM"
                     /locus_tag="PXB44_000010"
     CDS             7978..8223
                     /gene="nqrM"
                     /locus_tag="PXB44_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL5280540.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            8225..8641
                     /locus_tag="PXB44_000011"
     CDS             8225..8641
                     /locus_tag="PXB44_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5280541.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            8623..8934
                     /locus_tag="PXB44_000012"
     CDS             8623..8934
                     /locus_tag="PXB44_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5280542.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            9045..10208
                     /gene="mnmA"
                     /locus_tag="PXB44_000013"
     CDS             9045..10208
                     /gene="mnmA"
                     /locus_tag="PXB44_000013"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL5280543.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            10325..11704
                     /gene="argH"
                     /locus_tag="PXB44_000014"
     CDS             10325..11704
                     /gene="argH"
                     /locus_tag="PXB44_000014"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL5280544.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            11872..12870
                     /locus_tag="PXB44_000015"
     CDS             11872..12870
                     /locus_tag="PXB44_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL5280545.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            12971..14083
                     /gene="ispG"
                     /locus_tag="PXB44_000016"
                     /gene_synonym="gcpE"
     CDS             12971..14083
                     /gene="ispG"
                     /locus_tag="PXB44_000016"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL5280546.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            14089..15360
                     /gene="hisS"
                     /locus_tag="PXB44_000017"
     CDS             14089..15360
                     /gene="hisS"
                     /locus_tag="PXB44_000017"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL5280547.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            15370..15990
                     /locus_tag="PXB44_000018"
     CDS             15370..15990
                     /locus_tag="PXB44_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL5280548.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(16053..17156)
                     /gene="dnaN"
                     /locus_tag="PXB44_000019"
     CDS             complement(16053..17156)
                     /gene="dnaN"
                     /locus_tag="PXB44_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL5280549.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(17175..18524)
                     /gene="dnaA"
                     /locus_tag="PXB44_000020"
     CDS             complement(17175..18524)
                     /gene="dnaA"
                     /locus_tag="PXB44_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL5280550.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(18711..20558)
                     /locus_tag="PXB44_000021"
     CDS             complement(18711..20558)
                     /locus_tag="PXB44_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5280551.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(20627..21136)
                     /gene="luxS"
                     /locus_tag="PXB44_000022"
     CDS             complement(20627..21136)
                     /gene="luxS"
                     /locus_tag="PXB44_000022"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL5280552.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            complement(21154..23820)
                     /locus_tag="PXB44_000023"
     CDS             complement(21154..23820)
                     /locus_tag="PXB44_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL5280553.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(23903..24343)
                     /gene="fur"
                     /locus_tag="PXB44_000024"
     CDS             complement(23903..24343)
                     /gene="fur"
                     /locus_tag="PXB44_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL5280554.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(24379..24903)
                     /gene="fldA"
                     /locus_tag="PXB44_000025"
     CDS             complement(24379..24903)
                     /gene="fldA"
                     /locus_tag="PXB44_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL5280555.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(24909..25184)
                     /gene="ybfE"
                     /locus_tag="PXB44_000026"
     CDS             complement(24909..25184)
                     /gene="ybfE"
                     /locus_tag="PXB44_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL5280556.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            25425..26411
                     /locus_tag="PXB44_000027"
     CDS             25425..26411
                     /locus_tag="PXB44_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5280557.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            26508..27803
                     /locus_tag="PXB44_000028"
     CDS             26508..27803
                     /locus_tag="PXB44_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5280558.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            27923..28291
                     /locus_tag="PXB44_000029"
     CDS             27923..28291
                     /locus_tag="PXB44_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL5280559.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            28291..29484
                     /locus_tag="PXB44_000030"
     CDS             28291..29484
                     /locus_tag="PXB44_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5280560.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(29555..29773)
                     /gene="infA"
                     /locus_tag="PXB44_000031"
     CDS             complement(29555..29773)
                     /gene="infA"
                     /locus_tag="PXB44_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL5280561.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(29883..30542)
                     /locus_tag="PXB44_000032"
     CDS             complement(29883..30542)
                     /locus_tag="PXB44_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL5280562.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            30628..31491
                     /gene="serB"
                     /locus_tag="PXB44_000033"
     CDS             30628..31491
                     /gene="serB"
                     /locus_tag="PXB44_000033"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL5280563.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            31507..31998
                     /locus_tag="PXB44_000034"
     CDS             31507..31998
                     /locus_tag="PXB44_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL5280564.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            32083..32259
                     /locus_tag="PXB44_000035"
     CDS             32083..32259
                     /locus_tag="PXB44_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL5280565.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(32307..32603)
                     /locus_tag="PXB44_000036"
     CDS             complement(32307..32603)
                     /locus_tag="PXB44_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280566.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(32680..33648)
                     /gene="mdh"
                     /locus_tag="PXB44_000037"
     CDS             complement(32680..33648)
                     /gene="mdh"
                     /locus_tag="PXB44_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL5280567.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            33844..34305
                     /gene="argR"
                     /locus_tag="PXB44_000038"
     CDS             33844..34305
                     /gene="argR"
                     /locus_tag="PXB44_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL5280568.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            34355..35251
                     /locus_tag="PXB44_000039"
     CDS             34355..35251
                     /locus_tag="PXB44_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL5280569.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            35296..37494
                     /gene="uvrD"
                     /locus_tag="PXB44_000040"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             35296..37494
                     /gene="uvrD"
                     /locus_tag="PXB44_000040"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL5280570.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
     gene            complement(37641..37715)
                     /locus_tag="PXB44_000041"
     tRNA            complement(37641..37715)
                     /locus_tag="PXB44_000041"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37681..37683),aa:Gln,seq:ttg)
     gene            complement(37770..37844)
                     /locus_tag="PXB44_000042"
     tRNA            complement(37770..37844)
                     /locus_tag="PXB44_000042"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37810..37812),aa:Gln,seq:ttg)
     gene            complement(37870..37954)
                     /locus_tag="PXB44_000043"
     tRNA            complement(37870..37954)
                     /locus_tag="PXB44_000043"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37918..37920),aa:Leu,seq:tag)
     gene            complement(37957..38033)
                     /locus_tag="PXB44_000044"
     tRNA            complement(37957..38033)
                     /locus_tag="PXB44_000044"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37997..37999),aa:Met,seq:cat)
     gene            complement(38200..39024)
                     /gene="aroE"
                     /locus_tag="PXB44_000045"
     CDS             complement(38200..39024)
                     /gene="aroE"
                     /locus_tag="PXB44_000045"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5280571.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(39031..39585)
                     /locus_tag="PXB44_000046"
     CDS             complement(39031..39585)
                     /locus_tag="PXB44_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL5280572.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(39578..40114)
                     /locus_tag="PXB44_000047"
     CDS             complement(39578..40114)
                     /locus_tag="PXB44_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL5280573.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(40124..40435)
                     /locus_tag="PXB44_000048"
     CDS             complement(40124..40435)
                     /locus_tag="PXB44_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL5280574.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(40428..40904)
                     /gene="recX"
                     /locus_tag="PXB44_000049"
     CDS             complement(40428..40904)
                     /gene="recX"
                     /locus_tag="PXB44_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL5280575.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(40963..42069)
                     /gene="recA"
                     /locus_tag="PXB44_000050"
     CDS             complement(40963..42069)
                     /gene="recA"
                     /locus_tag="PXB44_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL5280576.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            complement(42170..42790)
                     /locus_tag="PXB44_000051"
     CDS             complement(42170..42790)
                     /locus_tag="PXB44_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL5280577.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(42768..43742)
                     /locus_tag="PXB44_000052"
     CDS             complement(42768..43742)
                     /locus_tag="PXB44_000052"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL5280578.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            43852..45459
                     /gene="rmuC"
                     /locus_tag="PXB44_000053"
     CDS             43852..45459
                     /gene="rmuC"
                     /locus_tag="PXB44_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL5280579.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            45522..46106
                     /locus_tag="PXB44_000054"
     CDS             45522..46106
                     /locus_tag="PXB44_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL5280580.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            46203..47285
                     /gene="prfA"
                     /locus_tag="PXB44_000055"
     CDS             46203..47285
                     /gene="prfA"
                     /locus_tag="PXB44_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL5280581.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            47397..47999
                     /locus_tag="PXB44_000056"
     CDS             47397..47999
                     /locus_tag="PXB44_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280582.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            47999..48865
                     /gene="prmC"
                     /locus_tag="PXB44_000057"
     CDS             47999..48865
                     /gene="prmC"
                     /locus_tag="PXB44_000057"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5280583.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            48874..49740
                     /locus_tag="PXB44_000058"
     CDS             48874..49740
                     /locus_tag="PXB44_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL5280584.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            49830..50684
                     /gene="kdsA"
                     /locus_tag="PXB44_000059"
     CDS             49830..50684
                     /gene="kdsA"
                     /locus_tag="PXB44_000059"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL5280585.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            50905..51852
                     /locus_tag="PXB44_000060"
     CDS             50905..51852
                     /locus_tag="PXB44_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL5280586.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            51950..53122
                     /locus_tag="PXB44_000061"
     CDS             51950..53122
                     /locus_tag="PXB44_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL5280587.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            53139..53822
                     /gene="lolD"
                     /locus_tag="PXB44_000062"
     CDS             53139..53822
                     /gene="lolD"
                     /locus_tag="PXB44_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL5280588.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            53819..55084
                     /gene="lolE"
                     /locus_tag="PXB44_000063"
     CDS             53819..55084
                     /gene="lolE"
                     /locus_tag="PXB44_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL5280589.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(55159..57090)
                     /gene="thrS"
                     /locus_tag="PXB44_000064"
     CDS             complement(55159..57090)
                     /gene="thrS"
                     /locus_tag="PXB44_000064"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL5280590.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(57257..58606)
                     /locus_tag="PXB44_000065"
     CDS             complement(57257..58606)
                     /locus_tag="PXB44_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5280591.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLIISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(58881..59372)
                     /locus_tag="PXB44_000066"
     CDS             complement(58881..59372)
                     /locus_tag="PXB44_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL5280592.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            59453..60220
                     /locus_tag="PXB44_000067"
     CDS             59453..60220
                     /locus_tag="PXB44_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL5280593.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(60211..61005)
                     /locus_tag="PXB44_000068"
     CDS             complement(60211..61005)
                     /locus_tag="PXB44_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL5280594.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(61066..62652)
                     /locus_tag="PXB44_000069"
     CDS             complement(61066..62652)
                     /locus_tag="PXB44_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL5280595.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(62838..64691)
                     /gene="dxs"
                     /locus_tag="PXB44_000070"
     CDS             complement(62838..64691)
                     /gene="dxs"
                     /locus_tag="PXB44_000070"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL5280596.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(64736..65548)
                     /gene="suhB"
                     /locus_tag="PXB44_000071"
     CDS             complement(64736..65548)
                     /gene="suhB"
                     /locus_tag="PXB44_000071"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL5280597.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            65729..66763
                     /locus_tag="PXB44_000072"
     CDS             65729..66763
                     /locus_tag="PXB44_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL5280598.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            66773..67657
                     /locus_tag="PXB44_000073"
     CDS             66773..67657
                     /locus_tag="PXB44_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL5280599.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
     gene            67796..68773
                     /locus_tag="PXB44_000074"
     CDS             67796..68773
                     /locus_tag="PXB44_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL5280600.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            68861..69892
                     /locus_tag="PXB44_000075"
     CDS             68861..69892
                     /locus_tag="PXB44_000075"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5280601.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            69980..70201
                     /locus_tag="PXB44_000076"
     CDS             69980..70201
                     /locus_tag="PXB44_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280602.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
BASE COUNT        20951 a        13919 c        15153 g        20333 t
ORIGIN      
        1 cctagccaga aggggagctt tttaggtaaa ataagaagat tatttttagt taaccttttg
       61 ttaagaaaag ataaaggttg atcacagatt tgagaactta atgtggcttt taaaaatagg
      121 tgggggacca ttgtttattt ttaagtaaag ctgcggctca atagaagcga ttgcgttagc
      181 gcaatcatat tttttaaccc aaaagaaaag tagtgcaaac aatatgatta caatcaaaaa
      241 aggcttagat cttccgattg cgggaaaacc tgagcaagta atccataacg gtaataccgt
      301 tactgaagtt gctatgcttg gtgaagaata tgtgggtatg cgtccttcaa tgaaagtgcg
      361 tgaaggcgat gtagtaaaga aaggtcaggt tctttttgaa gataaaaaga atccgggcgt
      421 ggtatttact gcccctgcaa gtggtaccgt aacggcaatt aaccgtggcg agaagcgtgt
      481 tcttcaatcg gtagtaatta aagttgaggg tgatgagcaa attacctttg ctcgttatga
      541 agccagccaa ttagcttcat taacttcaga gcaagtaaaa caaaaccttg tagagtcagg
      601 cttatggacg gctttccgta cacgtccgtt tagcaaagtg cctgcattag atgcagaacc
      661 ttcctctatt tttgtcaatg cgatggatac caatccactt gcagcaaatc cggaagtgat
      721 tattgcagaa caccaacaag ctttctttga tggcttaact gtattaagtc gtttatttga
      781 aagtaaaaaa gtgattcatc tctgtggcag tgcagactca aatattttaa atgcggcaca
      841 aagccgtcag atcaataatg tggaaactaa ccgctttaaa ggtccgcacc ctgcaggtct
      901 ttctggtacg catattcact ttattgaccc ggtaggtgca actaaacaag tttggtattt
      961 aaattaccaa gatgtaattg ctatcggtaa attatttacg accggtgagc tatttactga
     1021 ccgtgtagtc gcattagccg ggccacaagt gaaaaatcca cgtttggtgc gtactcgttt
     1081 aggggcgaat ctttcacaat taacagccaa cgaattaaat gcgggtgaaa accgtgtcat
     1141 ttcaggttct gtgttaagtg gtgctatcgc aacgggagta cacgactatt taggtcgtta
     1201 cgcattacaa gtgtctgctt tattagaagg gcgtgagaaa gcgttcttcg gtatgattgc
     1261 tccttactct gataagtatt caattacgcg tactacttta gggcattttg cgaagaaatt
     1321 atttaacttc acaacagcag ttcacggtag tgagcgtgca atggtaccaa caggggctta
     1381 tgagcgtgta atgccgttgg atatcattcc aacactctta ttgcgtgatt tagcagctgg
     1441 agatacagac tccgctcaag ctttaggctg cttagagtta gacgaagaag atttagcgtt
     1501 atgtactttt gtgtgttccg gtaaaaacaa ctggggacca ttattacgtc aagcgttaga
     1561 taagatcgag aaggacggtt aaaaatgggt ttgaaaaatc tttttgaaaa aatggaaccc
     1621 gctttccata aaggtggaaa gtatgagaaa tggtacacgc tatttgaagc tgcctatacg
     1681 attttctata caccgggtac ggtcactcgt aaagatgctc acgtgcgtga tgcgattgac
     1741 tctaagcgta tgatgttaat tgtatggtta gcgttattcc ctgcgatgtt ctacggaatg
     1801 tacaatgttg gtgcacaggg tattctagcg gcactcaaaa tgggaacatt aacggattta
     1861 gttgctaaca actggcacta cagcttagcc gatagtttag gctctgtagc tgatgcaggc
     1921 tggggaacta aaatgttgct gggggcaacc tatttcctac caatctactt aacagtattt
     1981 gcagtgggcg gtttctggga agtggtgttt gcaatggtgc gtaagcacga gatcaacgaa
     2041 ggtttctttg taacctctat cttactagca ttaattgttc ctccaacatt accgttatgg
     2101 caagcagccc ttgcaactac ctttggtgtt gtggtcgcaa aagaagtgtt cggtggcgtg
     2161 ggtaaaaact ttatgaaccc tgcgttagcc ggtcgtgctt tcttgttctt tgcttacccg
     2221 ggtcaaattt caggtgatgc agtatgggtt gccgcagacg gcttctcagg tgcaactgca
     2281 ctttctcaat gggcagttgg tggtcaaggt gcattacagc acgttgcaac cggtgaaacc
     2341 attacttgga tggatgcgtt cttaggtaat attccgggtt ctatcggcga gacctctacc
     2401 ttaatgttga ttattggtgc tgcaatcatt gtatttgccc gaattgcttc ttggcgaatt
     2461 attgccggtg taatgattgg tatggcggca actgcgacat tatttaatgt aatcggttct
     2521 gataccaacc cattattctc tatgccttgg cattggcatt tagtattagg cggttttgcg
     2581 atcggtatgt tctttatggc aaccgacccg gtatcagctg catttaccaa caaaggtaaa
     2641 tgggcatacg gtatcttaat cggctttatg tgtgtattaa tccgtgtggt aaacccagca
     2701 taccctgaag gtatgatgtt agcgatctta tttgctaact tatttgctcc aatctttgac
     2761 tatttagtag ttcaagggaa tatcaaacgc agattagcga gggtagcaaa caatggctaa
     2821 atttaataaa gatagcgttg gcggaacgct aaccgttgta gttttattaa gtttaatttg
     2881 ttctttgatt gtggcaggtg cggcagtaat gcttaaacca acacaagata ttcagaaaca
     2941 acttgataaa cagaaaaaca tcttacaagc ggccggttta ttgcaaaaag ataccaatgt
     3001 gcaagaaacc tataccaaat ttattgagcc gaaaattgtc gatttagcaa caggtgatta
     3061 cgttgagggt gtaaccggtt ttgatgctcg tgcagcagct aaagatcctg ctcaaaatgt
     3121 agttatcgca cctgaagatg ataaggcgaa tatcaaaacg cgtgctaaat atgctgaagt
     3181 ttatttagta aaagatgaat ctggcaaaac gactcaagta gtattaccaa tgcacggtaa
     3241 cggcttatgg tcaattatgt acggtttcgt tgcggtacaa cctgatgcaa ataccgttaa
     3301 cggtattact tactatgaac aaggtgaaac ggcaggctta ggtggcgaaa ttgcaaaccc
     3361 gaactggcag aaaaactttg taggtaaaaa actgtataac gagcaaaacc aagttgcgtt
     3421 aagagtgggt aaaggggcat ctgcagataa agatcacggt gttgatggtt tatctggtgc
     3481 aacattaacc tcgaacggtg ttgatggttc attcaaatac tggtttggtc aaaatggttt
     3541 tggtccatat ttagcaaaat ttaaagcagc ggcaggagct aactaatggc agacaataac
     3601 aatcttaaaa aattattgtt atcgccaatt gtcgataaca acccgatcgc attacagatc
     3661 ttaggtattt gttctgcgtt agcggtaacg actcaattac aaacagcagt agtaatggcg
     3721 attgcggtaa gtttagttac tgcattctca agtatgttca tttcaatgat tcgtaactat
     3781 attccaaata gtatccgtat cattgtgcaa atggcgatta ttgcatcatt agtaattttg
     3841 gttgaccaaa tcttacgcgc ttatgcttac gatctgtcaa aacaattatc ggtattcgta
     3901 ggccttatta ttaccaactg tatcgtaatg gggcgtgcag aagcattcgc gatgaaatca
     3961 ggcccggttg aaagttttgt tgatggtatc ggtaacggtt taggctacgg tgcaatgtta
     4021 attatcgttg cctttttacg tgaacttatc ggttcaggta aaattttcgg tatcaccatt
     4081 cttgaaactg ttcaaaatgg cggttggtat caagcaaacg gtttattctt attagcacca
     4141 agtgcgttct ttattatcgg atttgtgatc tgggcgatta gaacgtggaa accggaacaa
     4201 gtggagaaat aagatggaac atcttctgag tatctttgtt aagtccatat ttattgaaaa
     4261 tatggcactt tctttcttcc ttggtatgtg tacattcctt gcggtgtcta agaaagtttc
     4321 aactgctttt ggtttaggta ttgcggtaat tgtagtactt ggcattgcag ttccagctaa
     4381 ccaattagtt tatacccacg tattaaaaga gggtgcatta gtagaaggtg tggatttaag
     4441 cttcttaaac tttatcactt tcatcggggt tattgcggca cttgttcaaa ttctggaaat
     4501 gattttagac aagtttttcc cggcacttta tagtgcatta ggtatcttct taccacttat
     4561 caccgtaaac tgtgcgattt ttggtggtgt atcgtttatg gtacaacgtg agtataactt
     4621 tgtagaatct gtggtttatg gcgtaggtgc aggtacaggc tggatgttag ccatcgttgc
     4681 ccttgcaggt ttaaccgaga aaatgaaata ttccgacgtg ccggcaggct tacgtggttt
     4741 aggtattacc tttattacgg taggcttaat ggcgttaggc tttatgtcat tctcaggcat
     4801 tcaattataa ggagcatatc gtggatagta attttatttt cggtattatc gcatttacgg
     4861 ctctggtatt agtgcttgcg gtgatcattc ttttcgctaa atcaaaatta gttgattcag
     4921 gtgatattac catctcaatc aacaacgatc cggaaaaagg cattacgcta ccggctggtg
     4981 gaaaattact cggtgcttta gcaagtaaag ggattttcgt ttcgtcagct tgtgggggcg
     5041 gtggctcttg cggtcaatgt aaagtacaag tgaaatcagg cggtggtgaa attcttccaa
     5101 ctgagttatc gcacatttct aagaaagaag cgaaagaagg ctggcgttta gcttgtcagg
     5161 taaacgtgaa atcttcaatg gacgttgaac ttccggaaga gatctttggt gtgaaaaaat
     5221 gggaatgtac cgttatctca aacgataaca aagcaacctt catcaaagag cttaaacttc
     5281 aaattcctga aggcgaagaa gtaccgttcc gtgcgggtgg ttatatccaa attgaagctg
     5341 aacctcatac cgttcattat aaagacttcg atattccaaa agagtaccac gaagactggg
     5401 ataaatttga cttatggcgt tatacttcaa aagtggacga gcatattatt cgtgcatact
     5461 caatggcttc atatccggaa gagaaaggca ttattatgct taacgtgcgt attgctacac
     5521 ctccaccacg caatccggac gtaccgccgg gtcaaatgtc ttcctacatt tggtcattaa
     5581 aaccgggtga taaagtaaca atttcaggtc cattcggtga gttcttcgct aaagataccg
     5641 atgcggaaat ggtctttatc ggtggtggtg caggtatggc accaatgcgt tcacatatct
     5701 tcgaccaatt aaaacgttta aaatctaaac gtaaaatgtc attctggtat ggtgctcgtt
     5761 ctgagcgtga aatgttctat gtggaagatt ttgatggtct tcaagcagaa aacgataact
     5821 tcaaatggta tgtggcactt tctgatccat taccgggcga cagagaagat tacttccgtg
     5881 gctttattca taacgtgctt tatgagaact acctcaaaaa ccacgaagca cctgaagact
     5941 gcgaatacta tatgtgtggt ccaccggtga tgaatgctgc ggtaatcggt atgctgaaga
     6001 gtttaggtgt agaagacgaa aacatcttat tagatgactt tggtggttga taaaccttaa
     6061 cttctgacta caagcggtca aatttgcaaa attgaccgct tgatttaatt tatattatta
     6121 agttttggta atttaataat gtgaatttta aggggaagca aatggaacaa ctggatatta
     6181 cggtacttga aaatgccttc aattcccttg agaaaacgat tgtcagcctt gagaatcaag
     6241 cgtggtttaa ccggcaagaa gcaattattc aagacacttt gattgcagga gcaatccaaa
     6301 aatttgaatt tgtttacgaa ttgtcgataa aaatgctcaa gcgtcagctc aaaaacattg
     6361 agtcaaatga cactgatatt gatagcaccg agttccgtga tattttgcga atggctgctc
     6421 aatatgggct gattgaatca ccggaagttt ggctggatta tcgcaaaatg cgaaatatta
     6481 cttcgcatac ttatgatcaa aataaagcac agcaggttta taacggaatt tcaaactttc
     6541 tggaaagtac gtatttttta gttaaacagc tccaaaaacg caatagctat gattgattta
     6601 actgagtcac aacgccaaca ggtgttggat attttcaaaa aatacttgcc tgatacaccc
     6661 gtttgggttt ttggctctcg cattaaaggc acagccaaag cctattcgga tcttgattta
     6721 gcattaatca ccaaacaacc gctcacaatt cggcaacaaa cagagttaga gcttgcgttt
     6781 acggattctg acctaccttt cacagtagat ctgattgact gggcaagttg caacgaatcg
     6841 tttaaacaaa ttattttaca gcgatatgaa gtgcttattt aatcgctaaa tatgctataa
     6901 tttccccaat ttttaacttt tgtgacacca atgaaaaaat tttttcttct tttaattgct
     6961 ttttttactt taagtgcttg tgaaaaagcc cctgagcaaa ttacgctgtc aggcaaaacc
     7021 atgggaacga cttacaccgt aaaatatatt gataatggtg aagtggagaa tttgatttcg
     7081 cctgaaaaaa tgaaaaatga gctggatggc ttattgattg aggtgaataa tcagatgtcc
     7141 acctaccaac aagattcgga aattagccgt tttaatcaag caaagcaggc aaatcaagcg
     7201 gtcgaaattt cgcaggattt tgcaaaagtg gtacaagagg caatccgttt aaataaagta
     7261 accgaagggg ctttagatgt aaccgttggt ccattggtga acttgtgggg cttcgggccg
     7321 gataaacgct taaataaagt gccgtctgat gaacaaattg cagagcgttc tgcttatgtc
     7381 ggcattgata aaattttgct tgaaatgagt agtaaaccca agctcaccaa gtcagcagct
     7441 aatctttatc tagatttatc gtctattgca aagggtttcg gtgtagataa attagccgag
     7501 catcttgaaa ctataggtgt gagcaactat ttagttgaaa ttggtggcga attacgaggc
     7561 aaaggaaaaa atttacaagg cttagattgg cggattgcaa tcgagaagcc ggtaatgaca
     7621 caaggtcaag ccgcacaaat taccgtgccg ttgcacaatt tggcgatggc aacttcgggc
     7681 aattatcgta attattttga agatgaaaaa ggcaaccgtc tttcacatat tatcgaccca
     7741 aaacagttac gtccggtaaa ccataattta gcctctatca ccgtatttgc cccaactaca
     7801 atgacggcag acggcttatc aaccggttta tttgttttag gagcggaaaa agcattagca
     7861 gtggcagagc gtgaaaaatt agctattttt ttaattgtta aaaacggcga taaatttgag
     7921 acacaaatgt cgagcgaatt tagcaaactt attcaatcac aatagttgga gtgaaaaatg
     7981 gaaactgtat tattaacctt tggctttttt gtagccgtaa tttttgcgat gtcgattggt
     8041 tttatcgtga aaggcaaaac catcaaaggc agctgtggcg gtatcaccgc tttaggtatg
     8101 aaaaaaatgt gcgactgcga agaaccttgc gataacctaa aatctaaaat cgcagacggc
     8161 acagccgacc ctgaagaagt ggcacgtttt aataaagagc cacaatttta tgaagtgaaa
     8221 taatatgaat agagatattc gctggaaaca gcggtttgat aactataaaa aatcagtaag
     8281 ttaccttcaa gcagaagcag aaaaatacgc tgataccgac attgatgtaa ttaaaaaagg
     8341 aattatccag agttttgaga ttacacacga gttagcgtgg aagttaatgc aagatatttt
     8401 aaaatgggaa ggtgaagttg atatttatgg ttcaaaaacg gcttctcgtt tggcttttaa
     8461 tcgtgggcta atcactcagg gcgaagtttg gctagaaatg ataaaaagcc gcaatataac
     8521 agttcatact gacgatgaaa ctattttaac tgaagaattt aataaaatta tcaaggcgta
     8581 tctgccttta tttattgaat ttgaacagcg agtagaaaca ctatgccaaa ctttggacta
     8641 agcgaacgag cgacgaaaac tattaccgat attcttgcga aataccctga agtggagcaa
     8701 gccattattt acggctcgcg tgctaaaggt aattatcgtg aaggctcaga tgtagatctt
     8761 acttttaaag gaaaagatct tacttacgat attgtcaaac gcatttgggg tgatttagac
     8821 gattccgact cgccctattt atttgacttg tcgatttatc atcaattaac caatgccaac
     8881 tttattgaac atattgatag agtaggaaaa gtcttttacc aaagagaact ctaatttctt
     8941 aataggcaag cggttcattt tgcaaaattt tttgcaaatt tcaccgcttg ttgtttttat
     9001 tattaaccaa tgtgcttcgc cttaaccgaa gttacgggag atttatgaca actcaattaa
     9061 cctcaaaaac ctacgacact catttcccta aattaacccc agaacaactt gctgaaaaca
     9121 gcaagaaaaa agtcattatc ggtatgtccg gtggggtgga ttcctcggtt tccgctttta
     9181 ttcttcaaca gcagggctac caagtggaag gcttgtttat gaaaaactgg gaagaagatg
     9241 atgatacgga ttattgtacc gccgccgccg agcttgccga tgctcaagcg gttgccgata
     9301 aactgggtat gaaactgcat aaaattaatt ttgcagcgga atattgggat aatgtatttg
     9361 aatatttctt ggcagaatac aaggcaggaa gaacgccaaa tccggatatt ttgtgtaata
     9421 aagagatcaa atttaaagcc tttttggaat atgcggcaga agatcttggt gcggattata
     9481 ttgcgaccgg gcattacgtc cgccgtacga gcgatgatca agatgcgaaa cttttgcgtg
     9541 gcttagatga aaacaaagat cagagttact ttttatatgc gttaaataaa aagcagatcg
     9601 ggcagagttt attcccagtg ggtgaaattg agaagccgat tgtgcgagcc attgccgaag
     9661 acctaggatt agcaaccgca aagaaaaaag attctaccgg catttgtttt atcggtgagc
     9721 gtaaatttaa agatttctta gctcgttttc taccggctca accgggggaa atccgcaccg
     9781 ttgagggcaa attagtcggt cgccatgatg ggctgatgta tcacacgctt gggcaacgca
     9841 aaggcttggg tatcggtggg gtgaaagggc taagtgaaga tccgttttat gtggtggaga
     9901 aagacttaat taacaatgta ttgattgtgg cacaaggaca cgataattca gccctgcttt
     9961 caagtggctt aattgcgaaa cagctcactt gggtggatat gcagccgttg cgtgaaaatt
    10021 tacgctgcac ggtaaaaacc cgttatcgtc aagcagatat tgcgtgtgaa atccaagtga
    10081 ttgatgatga aaccattcga gtgatgtttg atgaaccgca aattgccgtg acgccggggc
    10141 agtctgccgt gttttactta ggtgaagttt gcttaggcgg tggcattatt gagtcacaat
    10201 taaaatagtt ataaataaat attgaaatat tttgcatatt tatgcaaaat aagcgaaaag
    10261 ttaaggcggt aaaacgcctt tttcattttg atcgatacaa agaataatat acaaggaaca
    10321 gactgtggca ctttggggtg gacgctttac acaacaagca gacgcaaaat ttaaatattt
    10381 taacgattca ttacgttttg attaccgctt ggcgattcaa gatattgaag gctcaatcgg
    10441 ctgggcaaaa gccattactt cggtaggtat tttaacggta gcagaattag aacaactaat
    10501 cgcagcatta aaagaggtga gagcagaggt agaatctaac cttaccatca tcttaaaaga
    10561 tgatgcggaa gacattcaca gctgggttga atctaagctg attgaaaaag tgggcgattt
    10621 aggtaaaaaa ttgcacaccg ggcgtagccg taatgaccaa gtagcggtgg atattaaaat
    10681 gtggtgcaag gtgcaagcgg tggcattgca agaaagaatc cgagctttgc aagagcgttt
    10741 ggtttccgtt gccgaagaaa atcaagagtc ggtgatgccg ggctataccc atttacaacg
    10801 agctcagcct gtgaccttcg ctcattggtg tatggcgtat tatgaaatgc tagaacggga
    10861 tttcagccgt ttgagtgatg ctaataaacg tatgagcact tgcccgcttg gttcaggggc
    10921 attggctggt acggcttacg gcattgatcg tgacttatta gcccgtgatt taggctttga
    10981 agaagccaca cgcaacagtt tagacagcgt gtcagatcgt gaccatattt tagagctgct
    11041 ctctactgcc tcgatcagta tgatgcacct ctctcgcttt gctgaagatt taatcatttt
    11101 taatagtggc gaatcaggct tccttgaaat gtcggatcgt gtgacctcag gctcatcact
    11161 tatgccacag aagaaaaacc ctgatgcttg tgagctgatt cgtgggaaat ccggtcgggt
    11221 atttggggca ttaaacggtt tattaactac actcaaaggg ttaccacttg cttataataa
    11281 agatatgcaa gaggacaaag agggtatttt tgatgcgttg gaaacttggc aggcttgttt
    11341 agaaattgca gaacttgtac tggttgatat tcaagtgaat accgaacgca cccgagaagc
    11401 cgctcagcaa ggttatgcga atgcgaccga gttggcagat tatttagtgg cgaaaggcgt
    11461 gccattccgt gaagctcatc atattgttgg cgaagcggtg gtttacgcaa tcagcaaaaa
    11521 agagccactt gaagcattaa gcattaacga attccagcaa ttccatcatt cgattggcga
    11581 tgatgtttac ccaattctct cgttagaatc ttgcttagaa aaacgttgtg ccaaaggtgg
    11641 agtgaaccca caacgtgtgg cagaggcgat tgcggtagcc aaagccaatt tagcttcaaa
    11701 gtaaaaccgg atacaagcgg tcatattttg caaaaaaatt gaaaaatatg accgcttgcc
    11761 gattaaattt tcaatttttt acgcattaag attgcaaaat aggcattacc tctttaaact
    11821 tccgaataat ttttgaattt tatttctact actttcttta aagagtttta tatgacggaa
    11881 tcagttaatt ctactcaagc tggtacagca ctttctttag ggcaacaatt aaaagcggct
    11941 cgtgaagcgt taaatctctc tattacagat gttgctcaaa aaaccaactt aaaaaaatca
    12001 catattgaat cacttgaaaa tgatatcttt attctacaaa atgttgcccc aacttttgtg
    12061 cgtggctatg tgcgtaatta tgtgaaattt ttgcgtttac ctgaatccct tatttcgagc
    12121 gttaattatg gtgaagtcac tattccaaaa gaagtcacta aagtttcttc agtaaaacct
    12181 tcatcaaatt cacaaggtaa aaccttaaag tatttaacgg tattagtatt gcttgcggca
    12241 ttaggaatga cattactttg gtggtggcaa gggtatcaaa aagaacagga aaatcgcgag
    12301 caatttgtta gctctgcacc agttgctgaa gtggcgaata ccgctacaaa taatgatgct
    12361 tcagcacaaa cttccgtgcc tgttcagcaa cctattcagg tttcagagca gcctgctcaa
    12421 gtagcagaaa cggtagttgc agcagaaccg gcattaactg aaaaagtaga gcctacacaa
    12481 gcagcatcat cacctgtaac acaagaagat gttaaagtgg tagaagaagt taaacaagct
    12541 gatgccacca ctgcacaggt tgaggcagtt aatgttttac agcaatctca atcggaacaa
    12601 gcccctacag aacagcctgt tgcggtaacg aatgatgagc ttcgtatcga aattaccggt
    12661 tcacaaagct ggattacagt gcggggtgct aaaaataaac gtttagcaga aaaattatat
    12721 aacaatggtg agactttaag tttcaatgag aatgagcaat atcgtttaac cattggtgcg
    12781 cctgccaatg taaaagtgta ctataaaggt caagaagtac cattgaaaat tgatgggcgt
    12841 gtagctcgta ttcatttacc gttacagtaa gcttgattta acgatgaaac gcacgtaaag
    12901 ctagcattta acgatagctg tctttacgag cgttttctgt ttttataaat tttcacaatt
    12961 agctaaaaat atgatacatc aatcacctat taaacgtcgg gtatcgaaga aaatctatgt
    13021 gggtaatgtg cctgtaggcg gcgatgcacc gattacggtt cagtcaatga ccaacacccg
    13081 cacaaccgat gtggaagcca ccgttgctca aattaaatcg ctggaacggg tcggggctga
    13141 tattgtgcgt gtgtctgtgc cgacaatgga cgctgctgag gcatttaaag cgattaaaca
    13201 acaagtgagt gtgccattgg tggcggatat ccacttcgat taccgcattg cgttgaaagt
    13261 tgccgaatat ggggtggatt gcttgcggat taacccgggt aatatcggga atgaagagcg
    13321 gatccgcacg gtggtagatt gtgccagaga taaaaacatt ccgatccgaa tcggggtaaa
    13381 tgccggatca ttagagcgtg atttgcagga aaaatatggc gagccgacac cacaggcatt
    13441 attagaatcg gcattacgcc acgttgatca cttagatcgc cttaactttg atcaattcaa
    13501 agtcagcgtg aaagcctcag atgtatttct agcggttgaa tcttaccgac ttcttgcaaa
    13561 acagattgat cagcccctgc atttaggtat tactgaagca ggcggagcga gagccggttc
    13621 ggtgaaatcg gcggtaggct taggtctgtt gctagcggaa gggattggtg ataccttgcg
    13681 tgtgtcatta gcggctgatc ctgttgaaga ggtgaaagtc ggttttgata ttttgaaatc
    13741 gctgcgtatt cgctctcgtg ggatcaactt tattgcttgc ccaacctgct cccgccaaga
    13801 aattgatgtg attgcgacgg taaacgagct agaacaacgt ttagaagaca ttatcacgcc
    13861 gatggacgtt tcgattatcg gctgtgtggt aaacggtcca ggagaggcgt tggtgtctga
    13921 tttaggggta accggcagca ataaaatgag cggcttttat ttagatggcg ttcgtcaaaa
    13981 agagcgtttt gacaacgcaa aattaatcga ccaattagaa gctaaaattc gtgccagagt
    14041 tgcacaacaa cataatcgta tagatattga acaaatagga taaatatagt ggcaaaaaca
    14101 attcaagcaa ttcgaggaat gaacgactgc tcaccaagtg agtctccatt atggcaatgg
    14161 gttgaacaga aagtgcgtaa tgtgctggca agttacggct attcggaagt acgtatgccg
    14221 attgtagaaa gcactccgtt atttgcccgt gccattggcg aagtaaccga tgtggtgtcg
    14281 aaagagatgt acacgttctg ggataacgat gagcaattaa cgctccgccc ggaaggtaca
    14341 gccggttgtg tgcgtgcagc gattgagcgt ggttggatct acaataatga gcaacgctta
    14401 tggtatatgg ggccgatgtt ccgccacgaa cgtccacaga aagggcgtta ccgccaattc
    14461 caccaagccg gtgttgaagt gtttggcatt gcaaatccgg aaattgatgc ggagttgatt
    14521 attctcaccg cacgcctatg gaaagagtta ggcatcgatc aacatgtttc tttgcaactt
    14581 aactctatcg gctcgttaga agctcgtgca aattatcgct cagcattagt aaaattttta
    14641 gaaaatcaca ccgctttaat gaacgaagaa gagaaagagc gtttggtgaa aaacccgcta
    14701 cggattttag ataccaaaaa cgaagcacta caaaatgtat taaacgatgc cccaaaactg
    14761 ctcgattatt tagatgatga cagccgtgaa cattttaccc aactttgtgc cttacttgat
    14821 gcaatgggaa tccgttacga agtcaatcca aaattggtgc gtggtttaga ctattacaat
    14881 aaaaccgtgt ttgaatgggt cacttcagcc ttaggctcac aaggcaccgt atgtggcggt
    14941 gggcgttatg acggcttagt cgaacaactc ggtggacacg caacccaagg cgttggcttt
    15001 gcaatgggct tagagcgttt agtgctgttg gtacaagagg tgaataaaga gattgattta
    15061 ccaaaaacgg tggatgtgta tatcgtattc tccggtgaag gggcaacact aaatgccttc
    15121 caacttgcag agcagattcg tagcgaattg ccacacgttg gcgtaatgac acactgttcc
    15181 ggcggtaaat tccaaaaaca atttaagcgt gcagataaag tagaagcgca aattgcgtta
    15241 gtgattggcg aaagcgaagt ggctgaaaag caagttgtta tcaaagattt acgttcagga
    15301 gcagaacaaa tcacgattgc tcaatcagag ctgattgctg agctatctaa acgattttag
    15361 gagaaaaaga tgagtaacga atatttaaat aaaaccgaag agcagcaatt tgaagatgcc
    15421 aaaaattggt ttaaagagaa cgggacaccg attttggtgg cgattatttt agcttgtgcg
    15481 gcaactgccg gctggaattt ttggaaaaat caccagcttg aaacggcaca aaaaacatcc
    15541 gcttcatacc aaagcgtgat ggagagttat ttgcaaaatc caagcaaaaa tcaaccgctt
    15601 gttgagaaat ttattgccga taataaagat tcaagctatg ccgcttttgc ccaatttgaa
    15661 gtagctaaac aagcggtcga aaaagggaat ttttctgcgg caaaaacagc gttacagtca
    15721 gttttgactt attcggattc aaccttacaa tctatcgctc gttttcgttt ggctcaaatt
    15781 gacttccaac taaaatctta cgatgaagca ctggcaagtc tggctcaaat tcaagataaa
    15841 gcgtgggaat tgcgtaagca aattctaaca ggcgatattc ttgcagcaaa aggcgatatt
    15901 cctgcggcga aaagtgctta tgagcaagct aaaacgaccg caaatcctca ggaacaaacc
    15961 ttaattgatg tacgtttgaa taacttataa aaacaaagcc tgattgaaat aaaatcaatc
    16021 aggctttttt gttggtttag tgttgctgtg aattataaac gcattggcat aatcacatat
    16081 tcggcagagc tgttgtcgca atcttcaatt aagcaactag aacttgaatc aactaagcta
    16141 aaacgcacac gttggcattt taaggtgttt aacacatcta atacatagct aacgttaaag
    16201 ccgacttcca tttccgggct gtcgtaggca acatcaatga tctcttccgc ctcttcttgc
    16261 tccggattgt ttgcggtaat tttgagtaaa ttattgctta aggttaaacg cacccctcta
    16321 aatttctcat tagaaagaat cgctgcacgc attaatgccc gtttaagggt ttcggtttcc
    16381 gcttctagaa tgcgatctgc attgcgaggc agaactcggc ggtaatccgg gaatctgcca
    16441 tcaatcagtt tagaggtaaa tacaatgccg ttcattgtaa tacggatatt gcttgaaccg
    16501 atttccaaac gcacggtttc atcgttgctg gcaaccaagc gggaaagctc aagtacggct
    16561 ttgcgtggca cgataaccga atgagtcaaa agctcttgat ttagcaccat tgtgcaaacc
    16621 gctaaacggt gcccatctgt ggcaacggtg cgtaataaat tgccttcggt ttcaaatttc
    16681 attccgttta agaaataacg ggcgtcttgg tttgccattg agaattgggt ggcatcaatc
    16741 agacgggcaa gggtagattg atcaatcgag aaatccactt ccggtttcca atccatcaaa
    16801 ttcgggtaat cacttgccgg taatgtagct aaactaaatt tactacgtcc tgctttgata
    16861 atggctttat cttcttcaaa ttgtaccgaa atcacgctgt catcaggtag gcttttactg
    16921 atatctagga attttttagc tggaatggta aattttccgg caaaatcgac cgcttgtagc
    16981 agttgtgctt gggtggttaa ttccacttct aaatcggtac cggttaaaga tagacgatta
    17041 ccttcaattt ctaataaaag attgttaata acaggcaaag tcggacggct acttaatacg
    17101 ccacacacct gttgtaaagg cttaagtaat tgttctctgg taatggtaat ttgcataatc
    17161 attccttcat cgggctaaga tgataatttg cgtgttaaat ggatataatc ttcttggata
    17221 ccgctttcgg tatcgcgtaa ttcattaatg gttttgcagg cgtgcatcac ggtagtatga
    17281 tcccgaccgc caaattcccg accaatttcc ggcaagctgt ggttggttag ctcttttgcc
    17341 agtgccattg ccatttggcg aggacgggca atgcttcttg cccgactttt cgatttaata
    17401 tcggcaactt ttatgccgta atattcggcc acaatgcgtt gaatattttc aatcgtaatc
    17461 aagtaatcaa aagaggcaaa cagatcttgc aaggtttcac gcaccgcatc aaccgtaatc
    17521 gggcgaaggg tcaatttttg ccacgcaatc acacggttta atgctccctc caattcacgc
    17581 acattggttc ggagtttttg ccccattaaa taggcggcat cttcttgcaa atctgctcca
    17641 cgttctaagg ctttttggcg aagaattgcc actcgggttt ccaattcagg tggctcaatg
    17701 gtcgcactaa taccgccgct taaacgggat ttaatcgctg tgtcaatttt atcgatattt
    17761 tttggcaaaa cgtcagaggt tagaataatc tgctttctct gctcaaataa agcattgagt
    17821 agatgtaaaa actcttcttg tactttcggt ttattggcaa gaaattgaat atcgtcaatc
    17881 attaacacat caagcgagcg gtagaatttt ttgatttttt ccgcatcgtt attggaactg
    17941 ttgatcgcct taataatatc acgataaaaa cgttccaaat gaatatatag cacccgagta
    18001 cccggtgtgc gtttaactaa ttcattgccg atagcgtgta ataagtgggt tttgcccaaa
    18061 ccggtgccgg cataaaacga gagtgggttg caatggcttt cgcctaaatt atccaccact
    18121 tgttgagcca ctaatttagc taattggttt gagcgacctt gtacaaaatt atcaaaggtt
    18181 tgatttggat ttagcccggt tttaaagccg gtattgctga attctgaact ctcgtttttt
    18241 agatttgggc tgacctttgg ctgttctgtc ggtttaatcc caacttttaa cgtaaccacc
    18301 aaattatcat ttttcgccaa aaaacgggca agctcggtaa tttccgataa aaatttatct
    18361 tttacccagt tttcaacaaa ttgattttgt gcataaagtg taagttcccc attctcagaa
    18421 ctcgcttgca gaggacgcag ccaagtgctg taatcagttg gtgagacttt cgtttgtaag
    18481 tgattaaggc aatcagacca aagggaagac acaatttgct ccaaaattat tgttattaaa
    18541 ggtcataatg atacctgaaa aaaggggaga aatcttgcaa atttttgaga gattttatgg
    18601 gggcttggaa gcgattttaa ttggcgaaca agcggtcgtt ttttgttgaa atcttgcaaa
    18661 ttgctaacaa aaaatgaccg cttgcttagt atgatgtgtg atggagttga ttaaatatcc
    18721 gtattctccg gcaagaaccc accgctttgg tgagcccaaa gtctggcgta aacaccgttt
    18781 tgagcaagca attcttcgtg cgtgccttgt tcaacgattt cgcctttatc taacacgata
    18841 agtctgtcca ttgccataat ggtagagagg cggtgggcaa tcgcaaccac ggttttacct
    18901 tccattaatt gcgtgagatt ttcttgaatt gccacttcta cttcagagtc taacgcactg
    18961 gttgcctcat cgagtaataa aatcggagcg tcttttagca tcactcgggc aatcgcaatc
    19021 cgttggcgtt gtccgccgga aagttttacc cctcgttcgc caacgtgtgc atcaaaaccg
    19081 gttctgcctt tggcatcttg caggtttgga ataaattcac tggcggcagc ttgttcaacg
    19141 gctttgatca tctcttcttc agtggcgttt gggcgaccgt acatcaagtt ttcacgcaca
    19201 gagcggtgta acagcgaggt gtcttgagtt actaaaccaa tttgtgagcg aaggctttct
    19261 tgggtaatat cacggatatt ttgcccgtca atcgtgattg aaccgtcttg aatatcgtaa
    19321 aaacgcaata ataaattggt gagcgttgat ttacctgcac cactacgccc gactaagccg
    19381 actttttccc ccggttttat agtgaggtca aaatctttca gtagcggttt tttcggatcg
    19441 taggcaaaat ccacattctc aaatttaatt tcgcctttgg ttacatttaa cggtttcgca
    19501 tcgattttat ctaccacatt gtgtggtttg gaaagcgtaa tcataccgtc ttgtaccgta
    19561 cctaaatttt caaatagacg agcaaattcc cacataatcc attgtgataa gcctttaatg
    19621 cgtaaagcca aagcggttga ggtggcaatc gcccctgcag ttactacgct tgaaccccaa
    19681 agccataaac cgatacccgc agttgaaatt attaatgcca tcgaggttag attagttacg
    19741 gtttcaatta cagtcactaa acgcatctgt ttatgcactg tcaccataaa tccttccatc
    19801 gactctttcg cataggcaga ttcacggttg ccgtgcgaga agagtttgac ggtggcaatg
    19861 ttagcgtaag catcggtaat tcgaccggtc attaaacttc tggcatccga ttgctcttga
    19921 gcagcttggg ctaatttcgg gacaaagtag ataatggttg ccgctaatga gatgacccat
    19981 aaaataaacg gcacgaataa ccagccgtca agttgtaata aaatcacgct ggaggtggtg
    20041 aagtaaacca gcacatacac caacatatcg gcacaagtca taatcacatc acgcacggaa
    20101 agggcggttt gcattacttt tgcagacact cttcctgcaa attcgtcttg atagaaacct
    20161 aaactttgcc caagcattaa gcggtggaag ttccaacgca aacgcattgg aaatacccct
    20221 tgcagagatt gaaagcgaat gctgctggca aggtaaacaa acaccactgc aagtagggca
    20281 ataaaaaaca taccgataat cgtccagcct ttttctgccc aaagttctgt tggcgagtat
    20341 ttattgaccc aatcgactaa atcgcccata aagcgaaaca gaaccgcttc gataataccg
    20401 acagctgcaa ctaatacggt cagtagtaag atataaaacc gcattccttt agtggcatcg
    20461 taaataaaag gaatgagacc tgattttgga gttttcggca tttcatttgg ataggtttcc
    20521 acccttgcct caaaccattt aaacagtttt tcaaacataa atttgtgatt tccgaataaa
    20581 aatgcagggg caaatagcca tttgcccccg aaaaaatgtg aaaaaattat ggattaagta
    20641 atttttcgtc taaggctaaa tcttcgttgc gatttatacc aatgccggca gctaatacgt
    20701 tgcgggcaat ttgatgtgct tcttttaaag aatgctcttt gtaagagcca cattgatatt
    20761 cattcagctc agggattgcc gatacatcag gaattttatg taacgcatct tccattgatg
    20821 ccgtccaagc tttgacaact tcttctgctg aaggcgatcc aatcagtgac atataaaaac
    20881 cggtacgaca acccattggt gaaatatcaa tgatctccac ttgttcgcta ttgaggtgat
    20941 cacgcataaa accggcaaaa agatgttcca tcgtgtgaat cccacgcacc ggtagaatat
    21001 caatattcgg acgacagaaa cggagatcga acacggtaat ggtatcgcct ttcggtgtgg
    21061 tcattgtttt tgcaacacgc acggctggtg cgttcatttt ggtgtgatct actttaaaac
    21121 tatctaataa aggcattttt tactccatca atattattcc gcaattcctt gatcccaagc
    21181 cggggcttca tcaggtagtt tgcgctgtaa gaaaaatcgt ctgtttggct gggcttgagg
    21241 cttcaccact ttagtgtaag gggtagagaa ctcaataccg ccatttaata actgcgacat
    21301 tgtgccggag ttcatactta caccttgcag gctcacgtcc atagtgtaac ctgaagcttg
    21361 ccagaattgg gtattggttc tgactaagtg cttgtattta tcttgaatat tgacataaat
    21421 aagcactcga tcgcctaact cacttagccc taaacggctg actattccca cctgcattcc
    21481 acggtaaagc accggtgctt ccggctgaat accgttagca ttggtggttt cgacaataat
    21541 ggggaaaccg ttggcatagg tggtttttac cgtatcggta tctgcaagaa caaattggtt
    21601 ttgagcttgc cctgaaccga catcaaccgt aatgtaattt tgtagtgctg catctaaatt
    21661 tttaaagccg gaggtactga tttcaggcga gataacatta aatttactgc cggcttttgc
    21721 aattaacgga tagtatttcc tttgaatgta agcggtcgct tttatctgtt tttttgcatt
    21781 ttgtagctca agtttttcaa tttgacctac ggttaagccc atatatttaa tgtccattcc
    21841 ttcgcttaac ttactcgcat cttttgcaat cagcgtgaga taagtattgc ccgaagtggc
    21901 tttctcctta cttgcataaa gcgttttatt gccttttgaa ccgccatcat caaagctgat
    21961 cgcccctttt aaggtacgca ttaatggagc tgtagtgata ttcacgcctt tgagtgacac
    22021 atcaacactc gttgccggct caatccagaa acggcttttc tcggttaata gatgacgata
    22081 atttggctgg ataaatagct caacctcaaa tttttgttgt ttcggggtta tttttaacac
    22141 ctcgccgacc tgaaaatcac gatatagcac caaagagcct tcgctgatac cagataatga
    22201 atctgccgaa agggtagtgg tggctttttt atctaaggta gccatgcctg attccgcact
    22261 ttctatatct ttataaagtg catattgttt tttcgggcta ccgtcatttt taccgttaat
    22321 taagcgaaca ccgcctttta accattcaga tggtgaaccg gcgtgaactc ttaacccatc
    22381 tagccccacc gacatatcaa tgttggatat cgcaacaaat ttgctgttac tcccaatcag
    22441 gttgcgataa ggcggataaa taatgacatc aaaacgtact ttgtctaact ctaggctacg
    22501 tttgacgatt tctccgattt gcacatcgct atagtaaatc ccctgattgg tatctacccc
    22561 ataggcttga ggagcaatta agctaaagct aagtaaattc ggtaaactta atagataatc
    22621 ggcttcgttt tgtaccacaa attccgattg aggttctcct tcccccgggt gaacctcaaa
    22681 atagacacca cgcagcagct cacctacttt cgtcagctgc tctgtgttta aggagaattt
    22741 cggctctttg agcaagattt tagtttggga acgtagtaaa ttttcataac ttgggtcaat
    22801 taataaaacc ccatcaatct gtttatgctg ccctgattct tgctgtggca caccgttgtc
    22861 ttttacttgc ggtaatttta gctgcgaaag cataccaatt tgcacatttt ggtgaaaaac
    22921 accagtctca ttaattttta aattatgtgt aagcggtagt tttacgttga tttcaattcc
    22981 acgtttagct gatttcaaat ccggatatag ctcaaatttc tgtccttgct cggcaatttc
    23041 catattatca ggcgaatcaa aagcaaccgc accttgtact accgaggcaa ggctgtccat
    23101 attgatttta ataccttgta aatttacatc agcgctaata ccgctaatat tccaaaatcg
    23161 gctctctttt tttactaaat gagcgtattt ttgattaatg acgacatcaa tttcgatttt
    23221 tttctggtct ttggtaaaac gataatcggc aatattaccc actggcactt tacggaaata
    23281 aacactcgct ccgacagtaa tagagcctat gtcatcagag attaaacgga tcagtaaatc
    23341 gccatcgctc acggtaatag ccggaggatc ttcttctgca ataaattcat caacgaaatc
    23401 gccttcaccg ggaagtaggg taatatagtt accagaaaca atcgcatcta agcctgaaac
    23461 gccagccaaa gaagcacttg gttgcactaa ccaaaattta gtctgtttgc gtaaaatact
    23521 tttggcttca gggtttattt ctgcttgcac ttccacttct tttaaattat cgacaaaata
    23581 aacacgctta acctgaccaa tctgcaagcc ttggtaacga atagcggttt ttccggcagt
    23641 aatgccatca cctttactaa atcgaatcgt gattgtttca ccttgctctt ttaaaatttg
    23701 gaaaaatagc aaagcaccaa taataaaagc aactattggt aacagccaaa aaggagaaat
    23761 tttgcgaggc tgccggattt gggcatctac aacagtgtgg gtattttctt ccatcgtcat
    23821 tttaaattag ggaaacctat atagaacaat accgaagacc caaagggact tcggtaatag
    23881 aaataagtca aaataagaaa tattattttt tctcatcaca gttggttaaa ttactgcatt
    23941 taccatacag atataaactg tggttagtca attccattcc gtgtttttca ctgatttcac
    24001 gttggcgacg ctccatatca ggatctttaa attcgaacac cttaccacaa tccatacaaa
    24061 taatatggtc gtgttcgtgg ttcatattta attcgaatac tgctttattc gcatcgaaat
    24121 tatggcgggt taaaatgccg gcttcttcaa attggtttaa gacacgataa accgttgcta
    24181 aaccaatgtc tgagcctttt tctaacagaa tcttataaat atcttctgct gcaaaatgtt
    24241 gcatttcatc acgaatatct tgcattaatg caaggatagt caaacgaggc tcggttactt
    24301 ttaagcctac ctcttttaaa cgtttaatat ttgcttcaga catatatgcc acttatttat
    24361 ctttatccaa aatagttatt atgcgagttg gtctaagcac atttcttctt tcaactgctc
    24421 gcaccattta ttaacacgtt cagtggttaa ttcaggctga cgatcttcat caatacataa
    24481 gccgacaaaa gtatcatcat caactaaggc ctgagaaacc tcgaaagtgt aaccggcagt
    24541 tggccaatga ccaacaatca caccaccatt tggctctacc acatcacgca cagtacccat
    24601 tgcatcacag aaatattctg cataatcttc ctgatcaccg caaccaaaaa ttgcaacgac
    24661 tttgccgtta aaatcaatct cttttagggt aggcataaaa tcgtcccaat cggcttgcga
    24721 ttcaccataa taccaagttg ggatcccaaa catcagaaaa tcgtatgctt caatatcttc
    24781 ttttgtgctt ttggcaatgt ctcgaatatc aattaattca gcccctaatt ttttctgaat
    24841 catttttgaa atattttctg tattgcctgt atcgctaccg taaaataaac ctacaactgc
    24901 cattgctact acctatttgt gagataatta tttagaattg tttcaattaa ctgcccgcgt
    24961 gaaacacctt gttcagacgc ctgttcctct aataacttta ctaaattcga atgcagtttt
    25021 aattctaccc gttttaaacc ggaagattta tctcgtttta gttggttacg tttattgatt
    25081 cgaagttgtt gttctctatc aagaggattt gtacgaggac gcccaacttt tggtacagtc
    25141 gcaaacagat ctaaggttat acaatctgaa tcttgttttg ccattttgtt ttactattgg
    25201 gctttagcta atcctcagtt ttaatgagaa tgataagcta cttttaaagt gtgaactata
    25261 tcaaaagttc aatcatttga aaagtaaatt acaggcaaaa caagcggtgt ttttaggttt
    25321 aaaatttgca aaaaccataa attacacatt atgagttatg atttttctga agcgtgaaat
    25381 tagcttataa tgaaaccgaa taacgttatg aaaaaggaga tagtgtgtcg aatatccatt
    25441 tagcgattgc cgctgatttt acgctggcag aaaaattact tgaagcatta gcaaatcaag
    25501 atttagggat tgaaaatatt tcagccgttg agcttgagcc gtttggtgaa gagcagaatt
    25561 tacgctttgg ggcaaaagca gtcgcacaat atgcgttaga tgaagtaaat tgggcggaat
    25621 ttagccatgt tctatttgcc ggcaaaatgg cacaagcgga agtgttggca gaagcggtga
    25681 aagccggctg tgtgatttta gatttatacg gcattaccgc actcatcggc gatgtaccgg
    25741 tagttgtgcc aagtgtaaat gatgaaaatg tcgcaaattt gcgtgagcga aatatcgttg
    25801 ctttagccaa tccgcaaatt tcacaactgg cgttagcctt aaagccattg ttgaacgagc
    25861 cgttaaaaca ggtgtgggta acctcacttt taccgtctgc ctattttggc gatgaaaaag
    25921 tcaaggagct tgccggacaa actgcccgtt tgttaaacgg cattccgttt gatgaaaatg
    25981 cggaacgcat tgcctttgat gttgtgccgg ctaatgtgca aggagaagag aaaagtctgc
    26041 cgttctcaaa agcctttgaa ctgcaaattg caaaagtttt accaaatttg accgcttgta
    26101 ctaccttcca cagcgtgcaa gtgccggtct tttatggggt ttcccaaatg gtgtcggtgc
    26161 agtctgaata tcagttagat gctaatgcgt taagtgaagc ttggaaagcc gaaaattggc
    26221 tgaattttaa tgaaaattca gtgattacag cggtgaaaaa tggcgaaaac gaagagcaaa
    26281 atgcgttaga aatcagtcag ttattggcga aatccgaaca ggaagtgcag ttctggacgg
    26341 tcgccgatga acagcgtttc agtctggcat ttttagcggt gaaattacta aaattagttt
    26401 tagcgtatta agtgcttgca aaagtattta ttccgtgata aaaatcgtca acgcccgagc
    26461 aaccgattgc ttgggcgttg tcattttgtt taaacaccga aggtcttatg ttagaaaaat
    26521 tatttcagct caaagcaaaa ggcagtaatg taaaaaccga aattatcgcc ggtattacta
    26581 cctttttcac aatggtttat attgttttcg tcaatccgtc tattttaggc gtggcgggaa
    26641 tggacaccca agtagtgttc gtgactactt gtttaattgc cgcttttggt actatcgcaa
    26701 tggggttatt cagtaattta ccgattgctc ttgcaccggc aatgggctta aatgcctttt
    26761 ttgcattcgt agtggtgcaa aaattaggtt actcgtggca agtgggtatg ggggcgattt
    26821 tcttaggctc tgtcggctta ttcctattaa ctgtattaca agttcgttac tggtttatgt
    26881 ctgcgattcc acttggttta cgtgtaggta ttggtgccgg tatcggttta ttcattgccc
    26941 taatcggttt taaaaatatg ggcttggtgg tgccaaatcc ggcaacgctt gtggcactag
    27001 gtgatttgca cgacccgaaa gtgttaatgg ggattttagg ctttttcatc attgttgtat
    27061 tagcggcgaa aggtattcac tcaggcgtgt taatttcaat tgtgattgtt accgctcttg
    27121 ccttagcctt tgacccggcg gtgagcttta acggcataat gtctatgcca ccaagccttg
    27181 gggcagttgt ggggcaggtt gatattgccg gtgcttttga tgttgcctta ctcgggatga
    27241 ttttctcctt tatgttggta aacctgtttg actcttccgg caccttaatt gcggtgacta
    27301 ccaaagcagg ctttgcggat gaacaagggc gtttcccgaa aatgaaacaa gctctgctag
    27361 tagatagtac cgctgcaatg gcaggctctt atatgggaac ttcagcgatt tctacctata
    27421 ttgaaagtgg ctccggcgtt tcagttggcg gtcgtaccgg cttaactgcg gtggtagttg
    27481 ggatcttatt cctattaacc attttcttct caccacttgc agggttagta cctgcctatg
    27541 caactgcggg ggcattggta tttgtaggaa ttttaatggc atctagctta attgaagtaa
    27601 aatgggacga tttaactgaa gctacgccgg catttattac taccgcaatg atgccgttta
    27661 cttactccat cactgaaggg attgcattcg gctttatcag ctactgcgta atgaaagcct
    27721 ttacagggcg ttggaaagaa atcaaccttt ctgttttagt ggtttcactt ctctttattg
    27781 ctaaatttgt gtgggtgggt taagctctac ccccctctcc ctgatttttc ttctaaacga
    27841 agaaaaatct atccctctcc cacaaagggg agagggtatt tgattcatta tctatgtgtt
    27901 tcgtgtattc cgaggtaaaa caatgtctaa ttcagttcaa aaacttcttt ttatcatcaa
    27961 ttctgctcct tacggcaatg aaacgttttt cagtgcgttg cgtttggcat tacaaattca
    28021 agaacaacat aaagccaata cggaaattaa acttttctta atgtcggatg cagtcaccgc
    28081 agggctggca aagcaaaatc cggcagaggg ttatcatatt cagcaaatgc tggaaatttt
    28141 aaccgcccaa ggggcaaacg taaaattatg caaaacctgt actaatgctc gagggattac
    28201 agatttgcct ttagccgatg gggtagagat tggcacatta attgaactgg cagattggac
    28261 aattcaagca gataaggtac tgaattttta atgcaaaata ttgatgttgt ggtgattggt
    28321 gccggtgcgg cagggctgtt ttgtgctgcc caactggggc aagctggcaa aaaagtggtg
    28381 gtgttagaca gcggtaagaa agtcggacgt aaaattttaa tgtctggcgg tggtttttgc
    28441 aattttacga atttagacgt aagtgccgga aattacctta gccaaaaccg ccattttgtg
    28501 aaatccgccc tttcacgtta tacccaatgg gattttattt cgcttgtcag ccactatggc
    28561 attagctatc acgaaaaaga gctagggcag ttgttttgtg atgaaagctc gcaacaaatt
    28621 gtcgatttac tacaagcaga atgcaacaaa ggcaaggttg agattctgtt acggcaagcg
    28681 gtcatttctg tggaaaaagt tgcaaatttt tttgaaattt cgaccgcttg tggcaagttc
    28741 caatccgaac acttagtgat tgcaaccgga gggctttcaa tgccggctct cggggcaagc
    28801 ccttttggct atcaacttgc cgagcagttt ggtattcctg ttgtgccacc acgtgcaagc
    28861 cttgtgcctt ttacttggaa agagagcgat aaatattttg ccgagctttc cggcattgca
    28921 ctgccgatta ctgtttccgc caatggcaaa agttttagca atcagatgtt gtttacgcac
    28981 agaggcttat ccggcccggc aattttgcag atttccaatt attgggaatt gggggaaacg
    29041 gtagagattg atctgctacc ttcgcagtcc attaccgata tattgagcga actacgccaa
    29101 tcttcgccga aattacagct aaaaaccgta ttatctcgtt atttacctaa aaaattggtt
    29161 gaagtttggc tggaacagaa attgctgacc gataaagtga ttgccgattt gagtaaaaaa
    29221 gatctgacct tattagagca atttatccac cattggcatt tcttgccaaa cggcacggaa
    29281 ggctatcgta cagcggaagt aaccaaaggc ggtgttgata ccgactatat ttcatctaaa
    29341 acgatggaag cgaaaaatac agcagggctt tattttatcg gcgaagtgct ggatgttacc
    29401 ggctggcttg gtggttataa tttccaatgg gcgtggtcat ctgcttttgc ctgtgcagaa
    29461 gggattattg aaaaaagcaa ataactaaaa aagcaaataa ctaaaaaacc aaacaaaaag
    29521 gcgaacacaa ttgttcgcct tattttatgc tgaattatct tgcacggaaa ataatgcgtg
    29581 ctttgcttaa atcgtatgga gtcatttcaa ccgttacttt atcaccggtt aaaatacgaa
    29641 tgtagttttt acgcattttg cctgaaatat gtgcggtaac aatatgaccg ttttctaatt
    29701 ccacacggaa cattgtattt ggtaaggttt ctaaaatcgt accttgcatc tcaatgcaat
    29761 cttctttagc catttattcc tctttaaagt tttaattttt atatgatgcc attcggcgaa
    29821 aaacggcgga tattatacgc ttattcgctc aaattacaat cgtttatcca tttgagtgta
    29881 aactatttac gcttaatcca acggcgacgt gagtgaaaac gctgggtttg ggtttttact
    29941 tgactcataa gtggttttgg gcttatctgt acatgatgaa ttttacgacg aaaatagtag
    30001 ttaatgctac ttgccaacaa tatacctgtg agtagcaaaa taattatttc cccataaagt
    30061 tgatgaaatt gttgctctac tcgtgcttta gtagtttgcc catattctgc atcaaacgtg
    30121 acgcgtaaaa agccaaccaa cgcctgttga gaaaatattg gctcaacaat ttgctgagtt
    30181 ttggcagatt gctctgaatc gagcaatact tttaaatctt tcgcattttg gctttgagcc
    30241 agtagcacgc catttggtga atagatattg gcatcaataa taaaatcttc tttggcaaaa
    30301 ctatccaacg catcaaccag ctcatcggtt ttggcatttt taactaacaa aaacgaaaaa
    30361 atatttgctt gctggcgaac caataggtgt gaaaggtttg atacttgatt aatgctggcc
    30421 aattgtgagc ctagcttaaa ttggtttacg cccattaaaa taaccgccac gatcccacag
    30481 cagagtagcg taatccctgc cagcaaaagc gttttcgtta ttttttcacg actaatgtac
    30541 atcgaaattc cctcaaaatg atgcgtgatt gtagcacgct tttctgttag attagtgcag
    30601 taaatttaaa caacctaaaa taagacaatg acaaacacaa tttttatcta ttcaaaaacc
    30661 ttaaacgagc gtgaaattga acagttttca caacaaacgg cagcaatttt gctgaaacaa
    30721 gcggtctatt tagactacaa aattgcattt ttttccacaa atttaaccgc ttgcgagctg
    30781 cgtgaaacgg cacagaaaat caatgcggat attagcgatc tcactattat tccaacctta
    30841 aaccaaggcg gtttgctatt aatggatatg gattccaccg ctattaaaat tgaatgtatt
    30901 gatgatattg ccaaacttgc cggcaccggc gaggtggtgt ccgccattac tgccagtgca
    30961 atgcggggcg agctggattt cgagcaaagt ttacgcaagc gagtcggcac gttggaaaat
    31021 gcatcggaaa gcattttgca aaaagtgcgg gaaaatctac cgcttatgga cggctttgag
    31081 cagatggttg ctattcttaa ggaacacggc tggaaattag cgattgcgtc cggtggattt
    31141 gattacttcg ccgattactt aaaacaaaaa tacgggttga attttgcggt ctctaaccaa
    31201 ctggaaatta ttgacggtaa attaaccggc aaggtgttag gcaaagtagt ggatgctcaa
    31261 cataaagccg ataccttaca gaatttagcg acaaccttta atattccacc aaatcaatgg
    31321 gtggcagtcg gcgatggggc aaatgactta ccaatgttaa aaaccgccag tttaggcgtt
    31381 gctttgcacg ctaaaccaaa agtgcaagca caagctgatt ttgtggtaaa cttcggggac
    31441 ttaacggcat tatgtttatt acttaatgcc aatcatttat ttcaacatta attgagagga
    31501 acaacaatgc catcatttga tattgtatct gaaatcacga tgcacgaagt gcgtaatgcg
    31561 gtagaaaatg ccaatcgtgt attaagcacc cgctacgatt tccgtggggt ggaagccgtt
    31621 attgagttaa atgaaaaaaa tgaaagcatt aagctcacga ccgaatcgga tttccaatta
    31681 gagcagttaa ttgagatttt aatcggctct tgtgtaaaac gtgggatcga gcatagttca
    31741 ttagatatcc ctacagaaag tgagcatcac ggtaaactct attccaaaga aattaagctc
    31801 aagcaaggga ttgagtcaga tatggcgaag aaaattacca agctgattaa ggattctaaa
    31861 atcaaggttc aagcccaaat tcaaggcgag caagtgcgtg taacgggcaa atctcgtgat
    31921 gatttacaag ccgcaattca gctagtgaaa ggggcagatt tagggcagcc attccaattt
    31981 aataatttcc gtgattagca attcctgcaa ttaatgatga caagtggaaa aattcagcaa
    32041 aatttgaccg cttgcattaa taattcaaca taaaaggaag agatgatgag cgagcaaaca
    32101 gaaaagaaag gctggtttaa aaaagccctt gagaaatatg atgccttttg cgatgagcta
    32161 ggggtaaatc aaggtgcttg ccgaggttgt gtccctgtgg tgaagtttga ccctgaaccg
    32221 gaaaaagagc agccagaaaa atcagaaaat aaaacataga aaaaatgcac aatggtaaga
    32281 tacattgtgc attttttata ggatttttaa ttggcgtgat gatgggcaaa atattgctta
    32341 actccgccat ctttcttgca aggattctga atatcagtag agtaactacc gttaaaacaa
    32401 agagcagtag cattcgatgg tttaatcttt tcctgtttag ttagggctgg cggttgagta
    32461 ttggtttcac taatatagat cgttttaggt tgagaatagt ctatattttt gtcgccattt
    32521 acgacttgaa cgggggtgtt cgagctgcaa gctacaaccg atgctgaagc caatgtaagt
    32581 ataatgatat ggtgtttttt catgggtaat ttccgttttt atatttttaa ttgagagaat
    32641 gttaaaaagg gctgatttct caacccttaa agttatgcct taagcatttt tattaaaagt
    32701 ttgtcctaac tgaatttcat catttaatac aggaattaac tgctctagtt gatctttttc
    32761 aaattcgctg agattgccga taggtagaac tttttccaca ccatttgtac ctaaacgaat
    32821 cgggtaagca aagaatgccg gagaggtttc gccattggca ttatctacat aagcataacg
    32881 aatgacatct tccccgatta aggctctcac taaaccaagt gcaaattctg ctcctgcttg
    32941 agccatagat aaggttgctg agccaccacc tgcttttgcc tcaaccactt cagtaccggc
    33001 gttttggata cgatgagtta attgctcgat ttgttcttga gtgaactcaa gttttaagcc
    33061 atttactgtt gcttgtgaaa gcagtggaag aatagttgtg ccggaatgtc cgccaatcac
    33121 aggcactctt acatatttca catttacgtc tttcgcttca gccacaaagg tattcgctcg
    33181 aataacatct aatgttgtaa caccgaaaag tttatttttg tcatacacac cggctttttt
    33241 caatacttct gccgcaatcg gaataatggt gtttaccggg ttagtaataa ttgcaatgca
    33301 agctttaggg caaactttcg ctgctttttc gactaagtta tgcacgatag tggcattcgt
    33361 attgaataaa tcggcacgtg tcatccccgg cttacgggca acaccagcag aaataacgac
    33421 cacatcggca tcttttagtg cctcagtcgg atcttcacct gcaaaaccgg ttacttttac
    33481 atcagttgga atatggctta aatccactgc aattcctgga gtaacagggg aaatatcgta
    33541 aagagataat tcagatttag ccggtaaatt gagttttaat aataaggcga gtgattgacc
    33601 gattccacct gcagcaccta aaagggcaac ttttttactt tgacacataa aatttctcct
    33661 aaatgttatg gaagtttaga ccaatttagt ctaagccaat tttttgggcg attcaaacgg
    33721 caaatctcat ttttgagaca tagagcaaat ttttagctga tttttatggg ttttattgat
    33781 ttatttgcat aaataagcaa gaattatgaa tatttttaag tttttgtgaa taagagtgtg
    33841 aaagtggaca agatagatag tttatcagaa gcgtttaaat cgctgttaaa ggaagaaaaa
    33901 ttcggctcgc aaagtgaaat tgttgccgca ctacaagaga tgggctttga taatatcaac
    33961 caatccaaag tttctcgaat gttatcaaaa tttggtgcag tcagaacccg taatcctaaa
    34021 atggaaatgg tgtatcagct accagtcgaa ttaagtgtac cgactacctc cagcccactt
    34081 aaaaatttgg tggtagatat tgatcataat gatgcgttaa ttgtggtcaa aaccagcccc
    34141 ggtgcggctc aactgattgc acgtttgctc gattcaatgg gtaaagccga aggtatttta
    34201 ggcactattg ctggcgatga tacgattttt ataacgccgg tacgcaatgt gcaaattgaa
    34261 aaattggtga agaatgtgac acagttattt gaaacatcat tatagaataa gtaaaggagg
    34321 acttaatgtt ctccttattt tttaggagat tattatgcac atttttataa caggtggtac
    34381 gggttttatt gggcgtgaat taatccagca attaaaatct gaaaatcacc actttacact
    34441 tcttactaga gatacagaag cgaaaagtga tgatttacag attaattatt gctattcact
    34501 tgatgaactt gttgatttaa atgattttga tgcagtaatt aaccttgccg gtgagccgat
    34561 ttttgataaa cgttggacag acgaacaaaa acagaaattg attgaaagcc gggttaacct
    34621 aacgaataag ctcagtcaat taatgaatgc aagctctaac cctccgcata cttttctctc
    34681 cggttcggca atcgggattt atggcgattt gcctccttct gcaaattttt atacagaatc
    34741 gaccgcttgt tatcaagatt gctttaccaa ccggctttgc caacagtggg aagccgctgc
    34801 actaaaagcc gcaagtgata aaacccgagt gtgtttaatt cgtaccggca cggtattttc
    34861 gcccgaaggc ggagcattaa agaaaatttt accgctttat cgatgcggat taggcggaaa
    34921 acttggttca ggcaaacaat attgggcgtg gatcagccgc cgggatcacg tacaagcggt
    34981 catttttctg ctcaaaaatg caaattgttc gggtgcttat aattttgttg caccaaatcc
    35041 ggtgagaaat gaagaattta atcgtatttt agcgaatgct gttaatcgcc cggcattctt
    35101 tactgtgcct gaatttgcct tgaaattagg cttaggcgaa cgagcttgtt tagtcttaga
    35161 taatcaacag ctcattccgg aaaaactact cgcagccagt tttgtattcc aagatccgat
    35221 tttaaccgag aacgttttct taaacccata atttaggcta taatgctgta taaaagcaca
    35281 gattaaaagg tgattatgga tttctcatta ttattagacg gtttaaatga caagcagagg
    35341 gaggcggttg ctgccccgat tggtaattat ttagtgttag ccggggcggg ttcgggcaaa
    35401 actcgggttt taacccatcg cattgcgtgg ctgattgggg tggaaaatgt gccggaatcc
    35461 aatattctgg ctgttacctt taccaataaa gcggcggcgg aaatgcgaca tcggattgag
    35521 tacactcttt cccaatcggg cgataatcga ttgttcggaa tgtgggttgg tactttccac
    35581 agcattgcca accgcttact tcgttcccac tatttagatg cggatttacc gcaagatttc
    35641 caaattatgg ataccgaaga tcagcaacgt ttactcaaac gtttactgaa actgcataat
    35701 attgatgaga aacattttcc gccaaaacac gttgcgtggt atatcaacgc ccaaaaagat
    35761 aaaggcaaac gggcaaaaga tattgatcac tataacgatc cgaatgaaaa aaaattggtg
    35821 gagatttacc aaatctatca agatgcctgc gaccgagccg gtttgttaga ttttgccgag
    35881 ctgttgattc gggcttatga actattcaaa accaaatcaa ccattttgca acgttatcaa
    35941 caacgttttc agcaaatttt gattgatgag tttcaagaca ccaacaatat tcagtacgac
    36001 ttaattcgtt tgcttgccgg cgatgtcggc aaagtgatga ttgtcggcga tgatgatcag
    36061 tctatttatg gttggcgtgg ggcacaggtt gagaatatcc aacgtttctt aaatgattat
    36121 gacaacgctc aaaccattcg cttagagcaa aattaccgct ctaccggcaa cattttaagc
    36181 tgtgccaatg aattaattgc caataacgat aaccgcttag gcaaaaattt atggacagat
    36241 agcggtgatg gcgagcctgt ggatatttac tgtgccttca acgaacttga tgaagctcgc
    36301 tttgttgcct cgcaaatcaa gcaatggaaa gaagacgagg gtgatttggc ggaatgtgcg
    36361 gtgttgtatc gcagcaacag ccaatctcgt gtgattgagg aagctttaat tcaagcgaat
    36421 attccttatc gcatttacgg tggaatgcga ttctttgaac gccaagaaat taaagatgct
    36481 ctggcttatt tacgcttaat cgccaatcgt caagacgatg cggcgtttga acgggtgatc
    36541 aacacgccaa ccagaggcat tggcgatcgt accttagaca cactccgtca aattacccgt
    36601 ggtcgccaaa ttacgctgtg gcaagcaatt caagtggcgg tgcaagaaga acaactttca
    36661 gggcgtgcca cttcttcctt attgcgtttt gtagagctga ttaatgctct tgatcaagaa
    36721 accgagcaga tgtcgcttgc cgaacaaacc gattttgtga ttaaaaaatc gggtttatat
    36781 gaaatgtata agcaagaaaa aggcgaaaag ggcgaagttc ggattgaaaa cttagaagag
    36841 ttagttaccg caactaagca gtttgtgaaa cctgacgaag cggaagagct gaccgatctt
    36901 accgcatttt taacccacgc ctcacttgag gctggagaag cccaagcctc gcaacacgaa
    36961 gattatgtcg aactgatgac gctacattcc gccaaaggat tggagtttcc ccgtgtgttt
    37021 attgtgggcg tggaagaagg gattttccca agcggaatga gctttgatga aggcaggttg
    37081 caagaagaac gccgtttggc ttatgtcggc ataacccgag ctaagaaaaa actgacgatt
    37141 agctatgccg aatcaagacg gctttatggt aaagaagaac gccacgttcc gtctcgcttt
    37201 attgctgaat tacctgaaga gcatattcga gaagtgagat tgagaggctc aattaaccga
    37261 gcggcaagtt ttaaccaagc taattcattt gcaaaaaatc cgacaaaaac gaccgcttcc
    37321 tttttaaacg atgatacttg gaaaatgggg cagaaagtga accacgctaa atttggacaa
    37381 ggcacgatta ttaatgtgga gggctcaggt gaagccaccc gtttgcaagt tgcctttgtc
    37441 ggcaacggca ttaaatggct gattgccaaa atggcaaatt tagagaggat ttaggcgaat
    37501 aaaaagtttt ttgcaggaat atagatggcg agatatattg ccccttaacc ttaaactaag
    37561 ggtaaataaa attaaataat gcaagctaga tataatgtaa agtatcagaa ataagatatt
    37621 aatttaagat aaagaataga tggctggggt actaggattc gaacctaggg atgccgagat
    37681 caaaacccgg tgccttaccg cttggcgata ccccaaaaga aaaaactaac ttacgttagc
    37741 ttatatagat ttgaatgttt gaaattaagt ggctggggta ctaggattcg aacctaggga
    37801 tgccgagatc aaaacccggt gccttaccgc ttggcgatac cccaacacta atttttgtta
    37861 atcgataaat ggtgcgggac gagggacttg aacccacaca cctcgcggcg ccagaaccta
    37921 aatctggtgc gtctaccaat ttcgccagtc ccgcaatggt ggctacagcg agattcgaac
    37981 ttgcgacccc aacattatga gtgttgtgct ctaaccagct gagctatgta gccatttgcg
    38041 ttcaccatat cggtcaacgg agtgcattat gctgatttta gggctttccg tcaaatactt
    38101 tttttaaaaa atttgaattt ttgattcgct tgtatagtta ttcgtcgttt tggcttaaat
    38161 ctagcatttt ttgtttataa aataatagct tatattctgc tatttcaccg tttgcatttc
    38221 taattttaat tgagccagta caggctcaac atcggggaat tgcccttccc acaaagaaaa
    38281 cgcaaacgct gcctgtccaa ccaacattcc taatccgtct tggcacgctg ttgctccaca
    38341 acgccttgca taattgagaa aaggagtttg catattgggg gcatattgca tatcgtaaat
    38401 attcgccgat gctataacag attcaggcaa ttcaacatat ttgccttgca gaccgagaga
    38461 ggtggcattg acgattaaat taaacagttg gtttgccaaa tcgcttaaac aagcggtctg
    38521 aattttgcca aattttgcaa atttttcggc taaaacgacc gctttctcct gcgtgcggtt
    38581 atatagagta atctctaacc ccgctttaag taacggaagt aatacgcctt tggttgctcc
    38641 acctgctcca agaattaagg ttttatgccc tttttgtaac caacctagcc gttccaaatc
    38701 agaaactaaa ccggccccgt cggtattatc ggcatataac cgtccatctt ctaagcgttt
    38761 aagcgtatta cacgcctgtg ccaacaaagc ggtttcgcta aattcatctg ccaatttaaa
    38821 ggctctttct ttaaaagggg cggtgatgtt gcaacctttt gctccagaag cgaaaaaatc
    38881 ggctaattgt tgctcaaaag ccgtttcatc tcctaagtta gcgatatatt caatgttgcg
    38941 ttgggtttgt aaagcaaaga gttgatgaat acggggggat ttactttgag caatcgggtt
    39001 gccccacacg gcatatcggt tcatttcggc ttatccttgt ctaaaaatct gttggctgaa
    39061 aatatctcga atttcggaag ggttttctct acctagcgtg tctgcgtgta atacaggaaa
    39121 atcttgacca aactgatcga tgacttcttt tgcattgcgg caaggtggtt ctccgctcaa
    39181 attggcactg gtagaggtca acgcaacgcc gcttgcctca cacaactgaa ccaccgccgg
    39241 cacattacat aaacgtacgg caatagaatc aaatttgccg gtgaggtaac ttggcacagt
    39301 atttttaacc ggtacgatcc aagtaattgc ctgctcgcca atatggctta ggcgttgcca
    39361 agcctgttcc gtcattcgag cttcatcaat atagggaaga agatacttta aatcagaagc
    39421 cagtaaaatt agcccttttt cctcagggcg ttgttttaat acgagtaatt tccttaccgc
    39481 tttttcactt tgtggattgc agcctaagcc aaaaaccgct tcggttgggt aagctacaac
    39541 ttcttcttgc tttaattgat taactatttc taaaaaatta ttcattcggt tttaattctg
    39601 tttggtgctg gcaacttttg ttagcacaaa tggtgaattg cttgccctta atttttttga
    39661 ttgtagcgag ttcacaacca catttttcac acaagtggtt aatcggtcga gttgctaatg
    39721 taaatttgca attagggaag cctatacaac cataaaatat tttgcctgaa cgcccttttc
    39781 tagcaatcag tttatgttgc ttgcattcag ggcaatcaaa ctcttcttca acctcagctt
    39841 cttcgtgtac gatgaagtgg cattcagggt aatggctaca gccgataaaa ataccataat
    39901 tgccttgttt taattggagg tttccgccac attcagggca aagttcatct aattgcttaa
    39961 taatatgacc ggcttgatgt aatggcttaa tataatcaca ttcagggtaa gctgaacaac
    40021 ccaaaaataa cccttttttg ccctgcttaa tttgcaacgg cgagccacat tgtgggcaag
    40081 tttcggtctg tttggttgat ttgaaaagta ccatattgat ttgctaaata agcgtaaatt
    40141 caatcacaaa aaacgcattg tcattcggat aaatctcccg aataacttgt ttcaattcgg
    40201 ctaagctcat attttcttgt ttagcgtgtt gctcgtttaa ttcatcaaac actataggtg
    40261 ttaccgatag cactttaata ttggcgaaaa atctatcggt ttcattagta aaaaccgcta
    40321 attcttggtt tggttgaaaa taagattctg acttgtctcg aatcgtaatt gtttttttac
    40381 cgcttaaaat atcggcttca aatcggctga aaaaggtaat tttctctcta ttcatattca
    40441 tcgctactgt aatccagagc cattaaatga ctaaaatgat cgttataaaa accacggcta
    40501 accataaatc gccacatttt ctgctttgct ttcagatccc atacaagcgg tcgttttttc
    40561 tcaaaaattt gctcagctaa ttcaaaccaa tccacttcgg cgttttctaa ttcttggtta
    40621 attaaccaat cttttacccc tttaagtttt agctcttgct ttaaacggcg aggcccaatg
    40681 ccttgcattg aacgataacg gataaaagtg gtacaaaagc gttcatcact ttgccactga
    40741 ttcgcttgag ctttttcaat cgctgcatca atctccgtga gttcatactc tttttgcttt
    40801 agtttttgcc gaagctcttg ctcgctgtaa tcacgctttg aaagtaaata tagcagataa
    40861 ttaaccgctg tgtatttatt tgttttagta gtagatgttg tcatttatcg gttataaata
    40921 aaaatgcgat tttcacgttg agcgaaaacc gcataaattt aattagagtt cttcttcact
    40981 aaattcatca tctgcattgt ctgcattttc agaatctgga gtagccgtaa aagttgggtt
    41041 tgaaatgagt agatttcgaa tatcttgctc aattttactc gccacttcag ggtgttcttt
    41101 taaccatttg atggcattat ttttaccctg accgattttc tccccttcat aagagaacca
    41161 agcccctgct ttattaatga agccgtgaga ttccgctaaa atgagtaatt cattcatacg
    41221 ggcaatgccc tcaccgtaca taatatcaaa ttggacttca cggaacggtg gagcaacctt
    41281 gtttttcaca attttcactt ttgtttcgct accgataacc tcatcaccgt cttttactac
    41341 gcctgaacga cgaatatcta aacgtactga agcatagaat ttaagggcat taccaccagt
    41401 tgtggtttca gggttaccga acattactcc aattttcata cgaatttggt taatgaaaat
    41461 caccaagcag ttggtcgctt tgatatttgc ggttaattta cgcaatgctt gtgacattaa
    41521 acgagcttgt aagcccatat gggaatcacc catatcgcct tcaatctctg cttttggtgt
    41581 aagtgccgca acagagtcca caataattac atcaaccgca ccggaacgaa ctaacgcatc
    41641 acaaatttct aatgcttgtt cgccattatc cggttgtgaa attaataatc catctgtatc
    41701 tacccctaac ttacgggcgt aaataggatc aagtgcgtgt tcggcatcaa taaaggcaca
    41761 agttttacca tttttttggg cttgagccac cacagataaa gttaatgtag ttttacctga
    41821 agactccggt ccataaattt ccacaatacg acccattggt aaaccaccaa tgcctaatgc
    41881 tgagtctaaa ccaagagaac cggttgaaac tgcttcaata tcaagggctt gagtatcgcc
    41941 taacttcata attgagcctt tgccaaattg cttttcaatt tgagctaatg cggctgccaa
    42001 cgctttttct ttttgttccg gatcaatctg tttaaccgga gtgttctttt gacttttttt
    42061 ctctgccata ttctatcctt tgtaaacttt tatctaaaag caaccgcttg ttgctgcaaa
    42121 ataactgtat attattatag tatttactta tccagttgca agcatttttt taggattttc
    42181 gatcaagatc acaaaaataa atttgcgagc aaggcaatgc ccttggttcc tcaaagggat
    42241 taagagcatt aatcaacata attttttcat attgatgcaa atcttgcttg gctattttgg
    42301 ctaaaacgac tttcccttca tcaagcaatt tactaagctg agtgcctttg agcaaataat
    42361 gttgcgggct ataccagatt ccttgtttta ggaacagtaa attgccgatg gagcaatcac
    42421 tgacgtagcc attattgaga ataatcacct cttcattttg taaaataggg agcaaatcaa
    42481 accgcttgcg gtcgctaaat ttgaggtgat aatcccaatt ttccacctca accaagcgaa
    42541 aacgttctac ttttttgggc gaatagataa aaaatttcag ctcaaattct gttgcgttgt
    42601 aatctattcg gcaacgtact ttgccccgtt gagaagcctc tggcacaact aaaatatcag
    42661 caaggctaaa acgaggttca cagccaaaat attcttgtgt tgcttgctca aaacgttgtt
    42721 gatgatactg taagttctga actttgccgt caataatcga aagggtttca aatagcggaa
    42781 atcggcacat agactttctc taacagttca ttatattcat cagttaacac actttgtgag
    42841 gtaatgccac caccactatg aaacacaagc ccgtttgagg tttgagcaat gtaacgaatt
    42901 gccaccgcac tttctaagct ctcgccatca aaataaccaa aaataccggt gtagtaaccc
    42961 cgttttccta attctgcttg ctgaataatc gaaaccgttt tctctttcgg tgcaccgcta
    43021 atggagccgg caggtagtaa ttttgccaat atcgaaccga tattttcttg ccaattttca
    43081 cctaattcgc cacagatctc ggaacttgtc tgcaaaattg cccctcgatg ggtttctact
    43141 ttttccacat aacggtattt agtaacttca atatttttcg ccaccaacgc caagtcattt
    43201 cggatcaaat ccacaatggt attatgttcg gtaaactctt tttccgaagc cattaatttc
    43261 tgctcggcat tctcctcatt tgcattaatt gtgcctttca tcgggtagga aaaaatgcgg
    43321 ttattttcaa ttcgcacaaa gcattcaggg gaaaaacaga cgaacctgcc ttgcaaataa
    43381 catttatatt ttgctttgct ggcggcaaaa atttccgcca atgaataatt ggtttcaatg
    43441 gcagtcgggt agctcaaatt cagcaagtag ctattgccgg cttgaatttc ctttttcact
    43501 aattcaaacc cggtttggta ttcgctaaac ggaatcggat ttgccgataa ttcaaaagct
    43561 ttagtcggtt tttcaaccgg cttttcgaga ttcgatttgc aaaaaaattc aaaaaaaaga
    43621 ccgcttgcgt gagcctgttc tagcggaaaa ataagtggct tttgctgctc aaaatcaatt
    43681 aaaaagaaaa aagggcgttt tgcttgcccg tagtgattgg cttggtgaat aaaatcttgc
    43741 atagaaaaca caatggctga aattggctga atcatcaaaa gctctgttat aatgcaaagc
    43801 aatagatttg aaaagcattt tttaaaaaag gttttaccga tgaacaacac cattcatttt
    43861 caagaaattg ggatctacat cgcccttgcc gtttgtgtgc tggtaggcgt ttatctcttt
    43921 ttcttgaaat cccgttatca gcgaaatgcc tacgaattaa gccaagatct tggcaaaatc
    43981 accgaaaatt ttgaacaaat tcagcaaaaa tatgaggtgt tggctcaaga aaaaaatcag
    44041 cttgagcagt gggcaattca acaacagacc aaatatgaag cagtgagtga acgtttaaac
    44101 gagcgagatg ggcaattgca acgattccaa caaaaaatcg aacttgccga acagcaagaa
    44161 aaccagctgg aacgctatat taatgagcta aaagaacgtg tgggttcttc ccaagcaaag
    44221 gcagaaagtt tggaagaaca gctacaattt agccaaagtt cactctcaac taaagagcgg
    44281 gaaaatcaag cgttatttag ccgtttaaac gaggtacaaa acgagctgac cgaactgcgt
    44341 actacgcttt cggaaaaaca ggcaaatttt gaagcacaac aacgcaattt tattgaggta
    44401 aaacaacagc ttaatgtgga atttcaacat ttggcacaac aaattcttga cgaaaaaagc
    44461 aagcgtttta gcgaaaccaa tcaaagctcg ttagaagctc tactgaagcc gttcaaagag
    44521 caaattgaag gctttcaaaa acgggtgaat gaggtacata gtgagtcgct caaaggctcg
    44581 gcaaatttag aggctgaaat taagcgggtg ctacaaattg gcgtttcaat gtcggaagag
    44641 gcacaaaatc tggcaacggc attaaaaggc aataataaaa ttgccggcaa ctggggtgaa
    44701 gtacagcttg aaagtgcctt acaatctgcc ggtttactcg caggcgagca ttaccaagcc
    44761 caagagagtt ttcgtgatga agaggggcga cgttttgccc ccgattttgt ggtgcatttg
    44821 ccggatcaaa aacatctgat tattgacagt aaagtctcgt tagtagcgta cgatcaagcg
    44881 gtcagatcag aagaaaattt tgcaatttct caagccttag acgaacattg ccgttcgcta
    44941 cgcaatcata ttgagggatt gtctaagaag aactacagtg cattattagg cattaaaagc
    45001 ccggattttg tactgatgtt tattccgata gaaccggctt atattgaagc gatgaagcac
    45061 gatccgcaac tgtttaactt tggctatgaa cgcaatgtga ttttggtttc tcacaccacc
    45121 ttaatgccga ttttacgcac ggtcgccaat ttatggcgga ttgaacgagg caacagcgag
    45181 gctcgtgaaa tcagcgaaaa agccggcgaa atttacaacc aagtgtgtgc ggttgccgat
    45241 cggttagcta aattaggtaa cacgctaaat accgcaaata atcaatacaa tcaaaccgtt
    45301 acctcgttag tgggacggca aggcttactg ggcaaagtgg aacgattcca acatctttca
    45361 agcaaagcaa gccaaattgt gccgcaggtt gagctgattg aaaatgaggt ggatactatt
    45421 aaattggaaa tggtatcgag agaattattg gaagaatagt caaatctctt tattccctac
    45481 aaaaatatgg ggtgatttga aatactaaag aaaataataa aatgctaaaa aaactattaa
    45541 tcatcgacaa ccacgactct tttaccttca acttggtgga tttactccgt aagcttgatg
    45601 tgccggcgaa agtggtgctg gttgaagagc ttgacctcaa tgaggtggag cattttagcc
    45661 atattttgat ttcgcccggc cctgatgtgc cgagagccta tctacaaacc tttgcgatgt
    45721 tggagcgtta tcatcaaacc aaatcaattc taggcgtttg cttggggcat caaacgattt
    45781 gcgagttttt tggcggcaag ttgtataacc taaacgatgt tagacacggg cagcaacgca
    45841 cgctcactca aattgagcct aaccccatat ttaaaggttt gcctaagcag ttgaaagttg
    45901 ggttgtatca ctcttggggc attttgcaaa attcgttgca aaatacaccg cttgtaccaa
    45961 ccgcagtttg cgatcaaaat gtgttaatgg cgtttaaaca ccaacatttg ccgatttttg
    46021 gcgtgcagtt tcacccggaa tcttttatca ctgaatttgg tcaacaaatg ttggcaaatt
    46081 ggcttgagat tacacaagat ttttagcgtg aaatccgcta taatccgtca gttatttacc
    46141 cttttaaaat ctgtaggggc gaaaaatttt tcgccttact taatatgcaa gagataattt
    46201 aaatgaaaga ttcgattatc aataaattag aaagtttaag tgaacgccac gaagaactac
    46261 aggcgttact tggcgatgcc tcaattattt ccgatcaaga caaattccgt gcttattcca
    46321 aagaatattc gcaattagaa gaagtggttg gcacctttaa ccgctggaaa aagctgaaca
    46381 atgatattga agaggctcaa ttattactcg atgatcctga tatgaaggat atggcagcgg
    46441 aagaaattgc ggaaaataaa gccgaaatcg agaatcttga gcaacattta caaattttat
    46501 tattgccgaa agatccgaat gatgaataca acgccttcct tgaaatccgt gccggaacgg
    46561 gcggtgacga ggctggtatt tttgccggtg atttataccg tatgtacagc cgttattgcg
    46621 aaagtaaacg ctggcgaatt gaagaaatgt cggcaaatga gagtgaacaa ggcggatata
    46681 aagagatcat cgttaaaatt tcaggcgaag gcgtttacgg tcagcttaaa tttgaatcgg
    46741 gcggacaccg tgtacaacgt gtaccgaaaa ccgaatcaca agggcgtatt cacacctctg
    46801 cttgcacggt agcggtaatg ccggagttgc cggaaagcga gatgccggaa atcaatccat
    46861 ctgatctgcg aattgacacc taccgctcat cgggtgcagg cggtcagcac gttaatacca
    46921 ccgactctgc agtgcggatt actcatatcc cgaccgggat tgtggtcgag tgtcaagatg
    46981 agcgttcaca gcacaaaaat aaagccaaag cgctggcagt gttagcctct cggattgtac
    47041 aagttgagca agaacgccaa gccgcagagc aagcggatac ccgccgtaac ttacttggct
    47101 caggcgaccg ttcggataaa atccgcacct acaactaccc gcaagggcgt gtgactgatc
    47161 accgtattaa cctcacggtt tatcgtttag acgaagtgat gaacggcaaa attgatgaat
    47221 tgatacaacc gattatcact gaatatcaag cggatcagtt agcggcgttg tcggagcaaa
    47281 attaatttta ttgtaagggt acactgtgtg tgcctgccaa atataaattt gcaaaatttt
    47341 gactattttt gatcgcttgt agtttctaaa aagtttaaaa atttgaggaa aacaaaatga
    47401 gaaagttagg tttattagca gtatttactt tagtattatc agcctgttca aattcaacaa
    47461 aaattgaaca aaccattctt tccaagcagg atatcgtagg ctcttgggta tgcgctatta
    47521 aatacgatga tctaaaagta agcactttag atttttctga atttaaagct aatggcgaaa
    47581 tgacgaattt cggtgatatt tctgatgaga attttgagcc cattaaattt acttatgtaa
    47641 caggagataa agggaagtgg gacttaaaag gaaatcgatt agtgattgat tatgatcttt
    47701 ctgaacgtaa agtaaaaaga acaacaccaa aaaaattttt agagttcttg aaacaggaag
    47761 agcgtaaagc ctcaaaaaca gctaaaaaac tccttaaata tgaacaaggg attttcaata
    47821 ttctgagtga tcagaaaaat actgaaaaca gtaaaataat attagatatt ttgaagtttg
    47881 gtcattcaag aatggcgatt cagcaaaata tgggagacaa agtttattca ggtggttgtg
    47941 taacagctga taaagcagaa gagtatttag atatcctaaa aaatagaaat tctaaataat
    48001 ggattaccga gagtggctca cttttgctga gcaaaaatta cttgaaaata gcggggcaga
    48061 tccataccta aacgcaaaat ttgatgcaaa tttgctattg caagcagtca caaaacgttc
    48121 aaaatcggcc atttttgctt tttcggaaac cctactttct gaaagcgagc ttgcagaatt
    48181 agcggaaaaa ttagctcggc gtgccaaagg tgaaccaatg gcatatattt taggctatcg
    48241 ggaattttgg tcgttgccgt taaaggtttc accggcaacg ctgattcctc gccctgatac
    48301 ggaacgactg gttgagttgg cgttggaata tgctaacaag cggttaggaa atcagaaaat
    48361 gttgcaaatt ctggatctcg gtaccggcac gggggcgatt gcgttggcac tggcgagtga
    48421 attaggcgag aaagcccaaa ttatcggggt ggatttccaa aatgaggcgg tgcaactggc
    48481 agaagaaaat cgccgaaact taggcttaaa tcaagttaca tttttacaaa gcgattggtt
    48541 cagttcgctt gcaaatcaac aatttgattt aattgtctca aatccgcctt atattgatga
    48601 atcggacgaa aatttgacgg tgggagacgt acgttttgaa cctttaaccg cattagttgc
    48661 cgataataag gggttgagcg atttgcaaaa aatcatcgaa aatgcaccgc ttcacttaaa
    48721 accgcaaggg gcgttaatgc tggaacacgg ctggaaacaa ggtaacttgg ttcgagattt
    48781 atttgattta cacttgtggg aacaggtaga aactgctcaa gattatgccg gcaatgaccg
    48841 agtaaccaaa gcgattttaa aataaggcag aaaatggacg atattattga agaattgtta
    48901 aaagagttgg aagatccgaa agtaaccatc agcgaaaccg agctgaaaaa gtttctttat
    48961 cgtgaaattt tacgattaac tacgctcatt gataagaatg taagtgagca acaagtgttt
    49021 ggtcagatgt ctgcgttggt gaaaaaagct cgctataacg tgaatggcga aggcgatgaa
    49081 gagagaatta accaattact tcatttagtc tatcgtgaat ggggctttca ttgctactat
    49141 gaagactatt tccacaccga aaatttgttg ctaaatcaag taattcgcaa acggcgtgga
    49201 atgccggttt cacttggggc ggtggcgctt tatttatccg ccgtattgga tttgccgctt
    49261 tacccggtta attttccgac tcagcttgtg ttacgggctg aaatacacca gccgaacggg
    49321 gcaaccaaag tgcgttttat caacccgtgg aacggtgaat ttctcaccat cgaaatgctg
    49381 aataaatggc tggaaggaga aatgggcttt ggcgtagaag ttacccctga tttgttaaga
    49441 cgagcagaac cacaagagtt gctggaaagg gtcgaaactg tgtttaaaat ggcacttacc
    49501 cgtgaaggca aatatgaaga aaccttacgc ttgattgaat accgcttggc atttagcccg
    49561 gaagatccgt acgaaatcag agaccgagga atggtactgg caagtatgga ttgctaccaa
    49621 gcagcattag aagatattaa ttatttcatt gaccaatgcc ctgaagatcc ttctgccgaa
    49681 attttaaaaa tggaagtgaa aggactgcaa caaaaaagcc aagaaagtgt ggtgcattaa
    49741 gctgagaagt agccccaaag gggctgagct atgattaacc tagtacggct ctgccgtgct
    49801 aggcagaaaa taagaaacaa ggactcccta tgacagataa aattgtaaaa gttggcaata
    49861 ttgaagtggc taatcacaaa ccgtttacgc tttttggtgg tatgaatgtg ctggaaagcc
    49921 gtgatatggc acttagagta tgtgagcaat atgttgaagt cacgcaaaaa ctaaatgtgc
    49981 cttatgtgtt caaagcctca tttgacaaag caaaccgttc ttcaatccac tcttaccgtg
    50041 gaccgggaat ggaagaaggc ttaaagattt tccaagaatt aaaacaaacc tttggcgtga
    50101 acatcattac cgatgttcac gaaatttacc aatgtaaacc tgtggcagaa gtagttgatg
    50161 tgattcaact accagcattt ttggctcgcc aaaccgattt agtggaagca atggctcgta
    50221 ccggtgcggt tattaacgtt aaaaagccgc aatttttaag cccgggtcaa atggggaata
    50281 tcgtagaaaa gattgctgaa tgcggcaacg atcaagtgat tttgtgcgac cgtggcacta
    50341 actttggcta cgataatcta gttgtggata tgcttggttt cggcattatg aaaaaagtgt
    50401 cgaaaggttg cccggtgatt tttgatgtga ctcactcatt acaatgccgt gatccattcg
    50461 gtgcggcttc cggtggtcgc agagatcaag taaccgaact ggcacgctcc ggtatggcga
    50521 ttggtttagc cggcttgttc ttagaagctc acccggatcc aaactcggcg aaatgtgatg
    50581 gtccatctgc attaccgctg tcaaaattag aagcctttgt ttcacagatg aaagcgattg
    50641 atgagttggt taagagcttt gaagaaattg atacttccaa ataaactttc tccgcattaa
    50701 atcctttcct actttctttg cttcgccaaa gaaagtaggc aaagaaaggc gaccctactt
    50761 cgctactttt cttcgcttag tgtgaatggg cttaacgaaa aatttacatt cgcttcgctc
    50821 agagtaaatt ttcctaccca ttaaaacacg ctcaggtagc tcagaaggga gttagaaagg
    50881 taaaagacag aataggagaa aaaaatgcta aatattgttt ttcttgaccg taccggtatt
    50941 cctgccactc acgatattcc tcgtccgaat tttccacata cttggacgga atatgccacc
    51001 acctctgccg atcaaacttt tgagcgggta aaagaagcgg atattatcat taccagcaaa
    51061 gtgttactcc agcgtgactt gctggctaaa ttaccaaagc tgaaattaat tgcgatcaca
    51121 gcaaccggca cgaacaatgt ggacttagcg gcagcaaaag agttagggat tgcagtgaaa
    51181 aatgtaaccg gctattccag cgttaccgtg ccggaacacg tcattggtat gatttactcc
    51241 ctgaaacatc gtttagccga ttatcaccgt gatttaatta cgtctgaccg ctgggcaact
    51301 tgcggccaat tttgctatgt agattacccg atccacgatg tgcgaggctc aaccttaggc
    51361 attttcggta aagggaacat cggggctgaa gtggcacggc tggcacaagc ggtcggaatg
    51421 caggttattt ttgcagaaca tcaaggcgca actgaaattc gagaaggcta caccgccttt
    51481 gaagaggtat tcaaacaagc agatattatt tcactacact gccctttaac cgagcagact
    51541 caaaacctga ttaatgcaga aaccttagcc ttaatgaagc cgaccgctta tattatcaat
    51601 acgggtcgag gcccactgat tgatgaacag gcattgcttg aggcattaga gcaaggcaag
    51661 attgccggcg ctgcgttaga tgttttagtg aaagagccgc ctgaaaaaga caacccttta
    51721 atgttagccg caaaacgttt accgaattta ctgattaccc cacacgttgc gtgggcaagc
    51781 gattctgcgg ttacaacact agtgaataaa gtgacacaaa atatggaaga gtttgtagca
    51841 acgggtatgt agcttgaagt aggggctggt cctgtgccag ccctaatatt aatagaatga
    51901 gagggcgggt ataaaacccg cccctaccaa tatcatttta gaaaaacaga tgaatttaac
    51961 cccttttttt attgcttttc gctattttag atcgaaaagt gctgaccgtt tcgggcggtt
    52021 agtcaccaat cttgccagcc ttggcattgt gctgggcgta atggcattgg tgattgtgct
    52081 gtcggttatg aacggtttgg aaaatatgca gaaaaccaat ctgctatcca ccttgccgca
    52141 cgctatcatc acccctcaag aggggcattt tgccaaaggt gaaaaactgg ctctacctga
    52201 atttgccaag caaagtgtgg tgattaaccg tgcgaatgtg attatccaaa gttcacaagg
    52261 cattaacgcc ggtcagttaa tcggcgtgga aaatgccaca gacgatccgt tgttagtggc
    52321 ggaaaatatc cccgcattat tgcctagcgg tgaatttaag gtagtaattg gctctcgttt
    52381 agccaataaa ttgaacgtta cggtaggcga taagctccgc ttaatgatca ctgaaaacag
    52441 ccaatatacg ccacttgggc gagtgccggt acagcgtttg tttgaaattt cgggcgttta
    52501 tcaatctaat cgtgaagcga gcgaatttac cctctttgct aatttagctg atgtcgggcg
    52561 tttgcttcgc atcagcgaag atcaagtaca aggcgtgcgt ttatatttgc aagatccttt
    52621 ccaagttacc gaacttagcc aatatttcga tgcagaacgc taccaaatca gtgattggcg
    52681 tgagcaaaaa ggcgaatttt tccaagcggt gaaaatggaa aaaaatatga tgggcttact
    52741 catcagcctg attattgtgg ttgcaatttc caacattgtg acttcactca gtttgatggt
    52801 ggtagacaaa cagggcgaaa ttgcaatttt acaaacccaa ggcttaacca aaaaacaagt
    52861 gatgcagatt tttgtgttcc aaggggcgat tgtcggagta attggctcga ttattggtgg
    52921 tttgctcggc gtattgatcg cctacaattt agatcagatt atcctgctat taaacccgag
    52981 catccatctg cctaccctaa tttcaggcac gcaaattgcg gtcattgtag gggtgtcgat
    53041 gttgctttca ctcctttgca cgctttaccc ggcataccga gcctcaaaaa ttgagccggc
    53101 acaggcattg agatatgaat aataagaacg gaaataaaat gacagaatta cttcgctgtg
    53161 aacatatcag caaattttac gatgagggcg aacagaaagt tcaggtgcta aaagaggtgt
    53221 cattttcgat gaacgaaggt gagctggtag cgattgtcgg tagttcaggt tccggcaaaa
    53281 gcaccctatt gcacacatta ggcgggctag atcagccaag caccggtgaa gtctggattc
    53341 gagggcaatc tctgcaacag ctcagttcag acaaattagc gttattgcgt aaccaaaact
    53401 taggctttgt gtaccaattt catcacttaa tggcagactt taccgccctt gaaaatgtga
    53461 tgatgccgat gctgatcggc aaacagaaca aaactgaagc tgccaatcgt gccgagaaaa
    53521 tgctacaagc ggtcggatta gctcatcgaa tgacacatcg cccatcagca ctttcaggcg
    53581 gcgaacgcca acgggtggcg attgcccgag cgttggttaa taatccggct ctggtacttg
    53641 ccgatgaacc gaccggtaat ttagaccaaa aaaccactga aagcattttt gagctgattc
    53701 aacagctcaa ccaagagcag aacattgcgt ttttattagt aacgcatgat ttaaatctag
    53761 cgaataaact ttcacgtagt cttgtaatgc gagatggcgt gttaagcgag gggagcttat
    53821 gagtacgccg tttttcatta gctggcgtta ccaacggggc aaacagaaaa acaaactggt
    53881 ttcactgatt gctctctttt ccaacatcgg tattgccctt ggggttgcgg tattaattat
    53941 cgggctaagt gcaatgaacg gctttgagcg agagctgaat aatcgtgtgc tttcggttgt
    54001 gccacacgct gaactggttt cgtttcaagg caatcagcct caaccaattt ttgatagcaa
    54061 aaaattagag gcattgataa aaaaatcgca aaatgtgacc gctagttcgc cttttgtcag
    54121 ctttaccgcc ttaattgaaa atggggctca gcttaaaatc gcccaggtgc gaggggttga
    54181 tcctaaaaag caagatcagg ccagccaatt aagccaattt attccacccg agcaatggca
    54241 agcgttcaat cagcaatttc aagccgatga agatggcttg attttaggag ccggtattgc
    54301 caaagcgtta gaagttgagg caggagacga agttacgctt ttaattcctc agccaacaga
    54361 agacggcaaa ttagcccagc ccttgcgttt taacctaaac gtaaccggca ttttgcggct
    54421 agatgggcag ctggatcaca gttatgcctt aattccactg aataaagctc aagagctgat
    54481 ggaataccaa gcgaatgaag tgtcagggct tgaaatcagc cttgccaatc cgtttgatgt
    54541 acaacagctt gaaatgccac aacttaacgg ctatacccaa ccgctttatt tgaatacgtg
    54601 gattgataaa ttcggttata tgtataacga tattcaactg attcgcaccg tgatgtacat
    54661 tgcaatggtt ctcgtgattg gcgttgcctg ttttaatatt atttccacct tgattatggc
    54721 agtgaaagac aaacaaggcg atatcgcgat tttacggaca ctgggggcaa ataacggttt
    54781 tatccgccgc attttcttat ggtatggctt aatttcaggt atgaaagggg cgttattcgg
    54841 gctgatttta ggtgtaattt tatcgctaaa tttaaccgct attatcaaag cgattgaagc
    54901 attctttgag gtgaaattgc tttcagacgg ggtttatttt gttgatttct taccaagtga
    54961 attacattgg caagatgtcg tctatgtatt ggcagccacg attatcctaa gcctttttgc
    55021 cagcttatac cctgccaacc gagccgctaa attagaaccg gcaaaagtgt taagtgggca
    55081 ttaatcattt tcctattcta ataaaaaagc agctacccct cgattatgtc agaaaggtag
    55141 ctgcttttat ttatgttatt actcttcgcc taataatttc aactcacgag ccttaatttg
    55201 gttttttaga atttcaacta agtctgaaac gaggtaagta cctaagtctg cacctttacg
    55261 ggtacgcaca gacactttac cttcggcgat ttctttatca ccgcaaacca acatataagg
    55321 cacacggcgt aaggtgtgct cacgcacttt gaagccgact ttttcgttgc gtaagtctgc
    55381 tttcacacga atgcctgcat cagaaagctg tttcaccact tgttgcacat aatctgcttg
    55441 gctgtcggta atgttcatta ccactgcttg ggttggggca agccaagttg ggaagaaacc
    55501 ggcatattct tcggtgataa tcccgatgaa acgttcgatc gagcctaaaa tcgcacggtg
    55561 aatcattacc ggtgtgcggc gaccattatc ttctgctaca taagaggctg ctaaacgacc
    55621 cggtagagcg aagtcaagtt gcactgtgcc acattgccat tcacgtccta agcaatcacg
    55681 caatgcaaac tcaattttcg gcccgtagaa tgcaccttcg ccttcttgaa tttcatattc
    55741 aagtccgttg ttgcgtaagg cgttggctaa gcctttttct gctctgtccc acattgcatc
    55801 atcaccgata cggttttccg gacgggttga aagttttact gcaatgttag taaaaccgaa
    55861 ggtgctgtaa atgtcgtaca ccattttgat acagctgatt acttctgact cgatttgttc
    55921 ttcagtacag aaaatatggg catcatcttg ggtaaaacca cgcacacgca ttaagccgtg
    55981 taatgaaccg gacggctcgt tacggtgaca cgaaccaaat tccgccatac ggatcggtaa
    56041 atcacggtaa gatttcaagc cttgattaaa gatttgaacg tgacccgggc agttcatcgg
    56101 cttaattgca tattcacggt tttccgattg ggtagtgaac attaaatctg cgtagttttg
    56161 ccagtgtccg gttttttccc aaagcacacg atccatcata aacggacctt ttacttcttg
    56221 gtaatcgtac tctttcaatt tggtacgcac aaaagtttca agttcacgga aaatcgtcca
    56281 gccgtcattg tgccagaaca ccatacccgg tgcttcttct tgcatatggt ataaatctaa
    56341 cgctttaccg atacggcggt ggtcacgttt tgccgcttct tccaaacgtt gtaaatattc
    56401 ggctaattgt tttttatcag cccaagctgt accgtaaata cgttgtagca ttttattttt
    56461 gctgtcgcca cgccagtaag cacccgctac tttttgtaat ttgaagtggt ggcagaaacg
    56521 catatttggt acgtgtggac cacggcacat atcaatatat tcttcgtggt gatataacgc
    56581 aggcgttgcc gtacgttcaa tattttcatc taaaatcgcc attttgtatg gctcaccacg
    56641 tttctcaaag gtatctcgag cttcttgcca gcttaccggg cgttttacca cgtcatagtt
    56701 ggtttttgcc agctctaaca tacgcttttc aagagcgtct aaatcttctt gagtcaaaga
    56761 gcggtctaaa tcgatatcgt agtagaaacc gttttcgata gtcgggccga tagccatttt
    56821 aacgtctggg aataattgct tgatggagtg acctaacaag tgagcggttg agtggcgaat
    56881 gatctctaaa ccatcttcgt ctttggcagt aatgatttca agtgtgctgt cttggctgat
    56941 gatatcagac gcatcacggc gttcaccgtt tacacgaccg gcaatggtgg ctttcgctaa
    57001 acctgccccg atagattggg caacttccat tacagaaact gggttgtcaa atgaacgttg
    57061 tgaaccgtcc ggtaaagtaa taattggcat aataaatcct tacagtggta atccatacga
    57121 gagattactt gtgttgtttg aatgaaaaaa ccgcctacaa gcggtctttt ttgattaaaa
    57181 ttttgcaatt tgcttattat ttcccttaca agtggggaat aaaagcactg aaattctagc
    57241 ataaaacaaa gaaaaattaa actttgctgt tttcagcggc cccttcatca agctcaatat
    57301 tttcattttc tgtttgtgga tcttgttcaa gtatttcaag ctgagcctgg tactcttgta
    57361 ccgcttcttc atcaaatgga ataagtgcgt cctcaacaac ttcaatttct tcaagtgggc
    57421 ttgcggttgc aattggggta gcatattgca tctctgctcg ttccgctttc ggaattgatt
    57481 tggtgcggaa agcacaaata aataaactaa gtgagaacag tccaaaaagt gcataaaagc
    57541 catattcagc ggaaaggctc atcgcaaaac cgataagcgg tggagcaact aacgccccca
    57601 caccatagca aaacagtaaa cccctgctta cttcaaccgt gttcatatta ttcggcaatt
    57661 catcgttggc acgggcaatg ctcaaagcat ataagctaaa cagactacag ccaaagaaaa
    57721 aggcgataat atactgtccc caggcggtca aattttcttg gaaaattgca aataatccgg
    57781 caagtgcggt taaagtggca atcactgaac aagctaaaat ggcgttacgt ctgccaatac
    57841 gattcgataa tatcgccatc ggcatttggc tgataaaacc accgatcatc gagataatca
    57901 aataaagcga gatttcactt aatgaaaagc taaacttcag cagaaagact gacgccattg
    57961 taaaaaagcc gtttaccaac attccgctaa taaagctggt tgctaaggct aacggtgaaa
    58021 tcgcaaatag tttaggaata ctaatgcgct ctcgagccgg aatatcaggc tgaaccattt
    58081 tagttaaagc aattgggatc atcgacatca caaccaaaat ggtagataag ataaaaatgt
    58141 ttcggctact aagttctaaa cttaataaac ttacgcctaa aataaaggcg agatagaaga
    58201 taatgttata agtggcaagc actttgcctc tggtatcgac attggttttt tcgcttaacc
    58261 aactttcgac taaaagcagc aagctataat gacagaatcc caaaataatt cgtaacacgc
    58321 cccagatcca caaagtatca atcataatat gaccgagagc ggcgatagca aaaacagcac
    58381 agaaaacgct aaatgaacgg atatgcccaa cacttgatac aatgcggtga gcaaaaatag
    58441 tgcttaatgc cgccccaata aagaaagcag cgttaagtaa accgatgatg atgtccgtta
    58501 tccccatttc tgccagtttg atccctgctg aatttagaaa taaaccgtag ccgaaaaaca
    58561 gaaaaaagat acctgaataa agggagtaaa atttagtgct attcatcaat gctccttcaa
    58621 acattctgcc agtattaaat cattgtaccg atttaatacc ttttaggcaa aaaagttatt
    58681 attagcgttg agaaaataac ttttggctat tttgattaaa aaatcaaatg aaggtgattt
    58741 tgaaatgtag taaaaaatag agcaatcaga attgaaaaag ggtttgccgt aacaaagcct
    58801 ttagaaagat tagagtttcg agaagaattt gtagcttggg ctaaccaaaa cacttgctag
    58861 gagtgcttta agcacaccac ctaaaataaa tggatataag cccgcttgta atactgtgcc
    58921 ttctggtaca aagaaagttg agccaagccg aaagcaaagg tgatcacact tgcaattaag
    58981 gcgacaaata aacttttcca aaaagagttt aatacgcctt tatcgctata atagcctaag
    59041 aaataggcgg ttggaatata gcctaataag tatccgcctg ttggaccaaa gaaatgatgg
    59101 aaacctgaag caccgccggc aaagacaggt aaaccaatcg ccccttcaaa aagataaagt
    59161 aatactgcag cagttgcggc tttacgaccc attgcaaaac caaccacagt taaagcaagt
    59221 gtttgccctg taatgggtac agggtacatc ggcacagaaa tttgtgcaaa aatagcaagc
    59281 agtgttgctc caataaaagt tttcaaccaa aatgtagtta tactattggc ttctagccaa
    59341 cgtttagttt gaattaattg cgtagtgaac ataatcctct cctttaatat ggttaaaatg
    59401 ttctcaaccg attatataag aagatgtctt ttagggtaag agtgaattta aaatggtaaa
    59461 accagtttat atttattgtg atgaaggtag cagtgaaatt ggtgttcaat cactgttatt
    59521 ggcagtaaaa cagcatttac agcgtccggc ttatctcatc aatgccaaac aaattattca
    59581 aagcaagttt tccgatggct gtttattaat catgcccggt ggagccgatt taccttactg
    59641 caaaaaatta aacggagaag gcaatcgtag aatccgccaa tttattgaac aaggtggagc
    59701 ttatctcggt atctgtgccg gagcttatta tggctgtgct gaattgaatt ttgtagggcg
    59761 tgactatagc gtaaatggta atcgagaact agctttattt aatggcattg cgggcattgc
    59821 gaaaggcagt ctgccgcagt ttaccaacgg gcaactttac gataaaaaca ttggtagcaa
    59881 agcgatagtt gaagtaacac ttgcagaggg tggaaaaatg ccgttttatt atcacggtgg
    59941 ttgctgtttt gaaccaaatt caacaacagt gatttacaac ccgttggctt attatcctga
    60001 tggctcagta gcaatgatag agggagagct agggcgaggt tactatctgt tgtcgggtgt
    60061 acattttgaa ttacaagcgg tcgtttattg gcaaaaaatt gtaaattgct gtgaaaatct
    60121 tgacataaaa acacaagaag aagcaatttg ctctaatttt aatgtaaatt atggctcgca
    60181 aatttggcaa catctaaagc aacgactaaa tcaagcgtaa aaaaataacc tttcgctatt
    60241 ctgccaaatg gtttctgcaa tcagatttgg tgattctgtt cttaattcac atagactatt
    60301 gaaagtcact aaaagccgtt ccggtcggtt cggctggcct tgaaagccaa acaccggcat
    60361 atcgggcgag tcggtttcca gtagtaaact ttcaagaggt agtttgcgga tagcctcacg
    60421 ggttttgtta gcacgttcat aggtaatagt tccccccaca ccaattttat aaccgagatc
    60481 gacaaaacgt tttgcctgtt catagctgcc ggcaaagccg tgaaccacac cggttttggt
    60541 tagttgggct tttttgagga acacaaaaag ttgatcgtgg cttttacgtg aatgaagatt
    60601 aacgggtaaa ttaaattttt tcgcaaggga aagttgtgcc tctaaaaatt cacattgctt
    60661 tttccataat tcaggggtag aaacttcact taccgctttt tccaaaccga tttcggcaat
    60721 agcggtacac tgtggatctt gttggcttag caaattttcc aataagatta aatcttgttg
    60781 ttggtgctgt tgaatataca gtggatgcaa ccctaatcca tacaccaaat tttccggttt
    60841 ttgtttggca caagcggtca cttttgcaaa attttttgca aatacggcaa cgataagaat
    60901 gcattcaacc tgatgctgtt tggcattctc aaccaattcc ggaatgctta aattcaggct
    60961 ttctgcgaga tagtcgaggt gagtatgagt gtcaaaaaat cgcataataa aaccaataaa
    61021 aacggggact agattgtccc catttttagc attttacgca agcaattagt ttgttacata
    61081 agcatcttca tcacgagcct cagatggctt gaggaaagca aagaagcaaa taacacaaac
    61141 taccgtaatc gcaaaaccga tataaactga taattcataa tctaaaccga agccgatttt
    61201 gttgttcgca aggtaagttg cacacacggt agtgataaac catgcaggaa tcgagcatac
    61261 ccagtggaat tttttatagc ggtataagta acctgcggca gtccataaca taaccattgc
    61321 agtcatttgg tttgcccaag tgaagtaacg ccataatacg ctgaaatcaa cttttgagac
    61381 aatgaaacca agtacgaata atggtacggc aatatacaaa cgttttacta aggtacgttg
    61441 ttctaaacca aagatttccg ctagttgtaa acgagcggct cggaatgcag tgtcacctga
    61501 ggtgattggt aatactacaa cgcctaatac ggcaaaaata ccaccgataa accctaagaa
    61561 gtgtaacgag ctgtcatata ccactttaga cggtgaaccg gcagagatcg catcttgtaa
    61621 ggcttgtggg ttttcataga atgaaagacc tactgcacac catactaatg caattacccc
    61681 ttcagtaatc attgcaccgt agaaaataaa acgaccttct ttctcatttt gcgcacagcg
    61741 agccattaat ggggtttggg ttgcgtggaa accggataat gctccgcaag aaatagttaa
    61801 gaatagtaat ggccaaattg gaacatcacc tttcacttgg aagttttggg tgaatttttc
    61861 ccacgttaag ccttgaccgt ctgcattcac tgtacggaag aagtcgatag gatcagtcgc
    61921 acttaagtga gcgctgacta gaccatatac catacctaca gacataaaga gtaataatgc
    61981 tccgaagaaa ggataaattc gaccgatgat tttatcaaca ggtagtaatg tagcaagaat
    62041 atagtaagca aaaatgatta acgtccacac agaaatcacg gttgctttat ccatacccca
    62101 aacggttacg ccacctgatg ctacagattc tttaattgct tcaacatcac ctaattccat
    62161 tgcactttgg gttgcacctg caatatccat ggtgatggta gagagtaact gagcagggct
    62221 tgcaacgaaa actacgccta ctaataaaag caagatgagc gccattacgt tgatgaataa
    62281 cttgactgga cgacccaaga atttccctgc cagatttggc atcgttgcgc caccgttgcg
    62341 gatacttaac ataccacaga agtagtcgtg tactgcaccg gcaaaaatac aaccaatcac
    62401 aatccaaagc attgcaactg gtccgtataa ggcaccaagt attggtccaa aaatagggcc
    62461 ggtaccggca atgtttaata attgaatcag ccaaatcttg gttttagaca ttggcatata
    62521 gtcaacaccg tcgttcattg cataagccgg cgttgctctt ttcgggttaa tcacgaagat
    62581 tttttcaatg atttttccgt aagtaaagta gccaagaaca agcacggcta cacagaaaaa
    62641 gaaccacaac atagtagaaa tcctataaat tgagataaat gatcattttt agcagaactg
    62701 ataaaggcgt gccattgtaa gtgaggagtg aggataaagt aaaatgaatt gttagatttg
    62761 tgtgaataat ttactgaaaa tgtgaggttc atcaaaaaat ccctttgttc tgcgtcaatc
    62821 aaagggactt tttaaagtta aatatcgaat ttttgaattt tcgcttcaat gccttgggca
    62881 tctaatccaa gatcggcata agcttcagct tgagtagatt ggggaacgaa aaagtcagga
    62941 ataccaagca ttacaagcgg tttgattttg ccgatatttt gcaaatattc attcacaaaa
    63001 ctacctgcgc caccttgaat agcattttct tcaagcgtaa ctaacaactc gtgtgaatcc
    63061 gccacttttt gaagtaatgc ttcatctaat ggtttaatga aacgcatatc taccaacgtg
    63121 tagttatgtt tttcagccac aatctttgct tcatcaagca aagcaccaaa atttaaaatc
    63181 gcaacttttt taccttcacg aataatgttt cctttaccta cttcaagtct ttgcattggc
    63241 tcaagtttta cccctttagc attgcctcga gggtaacgaa tagcacttgg cgtattcatc
    63301 gtgtaagcgg tataaagcat ttttcgcatt tcattttcat cgctcggtgc cataatggtc
    63361 atattgggaa tacaacgcat aaaacttaaa tcaaatgcac cttggtgagt ttgcccgtca
    63421 gcgccgacaa ttccggctcg gtcaatcgca aaaatcaccg gcaggttttg aatagcaaca
    63481 tcgtgaatta attggtcata agctcgctgc aagaagctgg aataaattgc gactaccggt
    63541 ttgtaacctg cgattgctaa gcctgccccg aaagtcactg cgtgttgttc ggcaatcgcc
    63601 acatcaaaat attgttcagg gaaacgtttg gaaaattcta ccattcccga gccttcacgc
    63661 attgccggcg taatgccgat aattttcgga tcacgctctg ccatttcaca tagccaatcg
    63721 ccaaaaatat tggaataagt aggtgctgct tttgattttg gtaactcggc tgcggtaggg
    63781 tcaaatttcg gaacgccatg gtagccaatc ggatcatttt ccgcaggtgt gtagcctttg
    63841 ccttttttcg tgcgaatatg cagaaattga gggcctttca gctcacgcat attgctcaag
    63901 gttttgacta actcgtcaat attatgtcca tcaatcgggc cgatataatt aaagcctaac
    63961 tcttcaaaca aagtgctttc aggggagaaa atcacccctt tcatatgctc ttcgcttttt
    64021 ttcataaagt ttttgatggt aggcacttta tctaaaattt tcttactgcc gtcacgcact
    64081 gaagtataga gcgaaccaga aaaaatgcgg gcaaggtggt tgtttaatgc cccgacattt
    64141 tcagagattg acatttcatt atcatttaag ataaccaaca tatcggtatg caatgcacct
    64201 gcgtggttta atgcttcaaa tgccattccg gcagtgattg caccatcacc gattacacag
    64261 acggttttac ggccggcatt ttctttttct gctgccaccg caatgccaag tccggcactg
    64321 atcgaagtag aagaatgccc aacgcttaac acatcgtaag ggctttcttc acgccaaggg
    64381 aaggggtgaa taccatcttt ttggcggata gtgtgcattt gatctcggcg cccagtaagg
    64441 attttatgtg ggtaagcttg atgaccaacg tcccaaatta attggtcgaa aggcgtttga
    64501 taaacataat gcaaagctac cgtaagctct actaccccta agccagaggc taaatgtccg
    64561 ctagtttgac tgactgattc caataaatag gctcgcagtt catcaactac ggtttgaagc
    64621 tggtctttag aaagtaaacg taaatcttcc ggtgagttga tttcgcttaa cagaggatac
    64681 ttcttttcca taatgtttta ttacccacaa ccatcaaata atagatgata ttaaattaag
    64741 ctttgagttc ttcggtcaaa gtaggctgaa tgctatttaa aatttcttta accacacggc
    64801 cgttaccaac aaccagatta cctgttttca agtaatctgt tccgccaaca aagttagtca
    64861 caattgcacc ggcttcacgg gcaataagtt caccggcggc gaaatcccac ggttttaagc
    64921 cgatttcaaa gaaaccatca acacgacccg ctgcaacata ggcaagatct aaagcggcag
    64981 aaccggtacg gcggaaatca gctactccgt tattcattaa gccttcaagc atatttaaat
    65041 gtgcatcacg atgtctgatg actttgaaag gaaaaccggt tgcaattaca gtgcctttta
    65101 aatcacggcg ttctgcatca atgcgtaaac ggaactcgtt taatttagcc ccttcgccac
    65161 gcaccgcagt gaaaagctcg ttacggatag gatcgtaaac tacccctact gttgtacgtc
    65221 cgttttcacg cacggcaatc gacacagcaa aatgaggtaa gcgttttacg aaattggttg
    65281 taccatctaa tggatcgatc acccattgta cattttcatt gctaccgttt aacacgccgg
    65341 actcttcggt cacgatcgag tgttcagggt aagatttacg gatcacatca ataattgccg
    65401 cttccgctgc tttatcgatt tcagtcacat aatcattcgt atttttttgt aaaacttgtg
    65461 tatcttgagg cgattgttca tagcctttgg caataatatt gcctgctttt cgtgccgcac
    65521 gaatagcaat gtttaacatt gggttcatat ttccaccaga ttttaaagaa cataataaag
    65581 cacgaattat aaagaatgta gcgaaataaa gaaacaacaa gcggtctaat ctgtgaaaaa
    65641 ttttgcaaaa aaatggcttt ttctaaccgc ttggagtttg gattcgttat aatacctccc
    65701 attcaagcca cccatttagg tactaaaaat gcaatttaaa gaaaataacc aacctcgttt
    65761 taaacacaga gaagaacgcc gttcagatga gtgttccccc cgtttttcag ataaaggcaa
    65821 aatgcgagga aatgaaggta aaagtgacag accgcataag ctacatttta agcgtgacga
    65881 ggcttattca cgctctgagc agatttatcc agcagaaggc aaaaaagcca gtggtgaagg
    65941 tagtgtgcaa atttgggtaa aaggtgggaa tagctcagtt aaagagaaaa aaaacggccc
    66001 gctttcaccg agagcgcctg agaaaattaa gaaaaatcgc ttggaagaga tgaaagtcta
    66061 tggcgaaaat gcctgtatca gtttgttcca acaacgtcct gaatctattg tacgtttatg
    66121 ggcaacagtg gagggggcaa aaaaattagg cgatttaatg agctatttgg ctcagcacaa
    66181 aaaagcctat cacgttattg accgtgaaga gatggagcga gtcaccggta ctgaacatca
    66241 cggcgatatg tgcttattgg tgaaaaaaac ggcttcattt gtgctagagg gctatttaca
    66301 agtgccgaaa aaacaagatt gcttggtact gttagatggt gtaaacaatg ctcagaatgt
    66361 ggggggaatc atcagaacgt gtgcttttta tggtgtgaaa ggcattatta ctgaaaatgt
    66421 ggatagctta aactccagtg ctgcagctcg tgtcgcagag ggtggtttgg aatttgtgcg
    66481 agcgttagaa actaaaaaca agcatattgc tttggtacaa ctccgccaag ccggttatca
    66541 aattgtccac ctcacacgcc ataagcaagc cccttcttta accaaaatta agctggcgga
    66601 aaaggtggtt tttgtattaa gtgagattcc tgcaagtgat gtggcttacg cagaagacac
    66661 aacaatacag ctttccttta ataatccgtt aaatagtggt ttaaatgtgg cggtaaacgc
    66721 aggtattcta cttaatcaat ggtatcaaac ccacattctt taaggaattg aaatgaatca
    66781 gcaaaaaccg tttgaagtga ttgcgattgt tggcacacct cgccacgaca gtgcatttga
    66841 aacacatctt gccgtttata attggttaaa agatcgtgat tatcaaattt tagtggaaac
    66901 agaggttgcc actcagttac agcttaagga aagctacagt ttagatcaga ttgggcagcg
    66961 cgcagagttg gtgattgtga taggtggcga tggtaatatg cttggcatcg cccgagcttt
    67021 ggctaaatac cgagtgccac tgataggcat taaccgtggc aatctgggtt ttttaaccga
    67081 tatcgcacct caaacggctt ttgaacagct ttatagttgc ttagtcaaaa atgagtatgt
    67141 gattgaagag cgattcctgt tagaggcttt cattgagcgc aacggtaaaa ttattgccgc
    67201 aaataatgcc ttgaatgaaa ttgtggttca cccaacccaa gtggctcgaa ttattgaatt
    67261 tgaagtttat attgacggaa aattcgcttt ttcacaacgt gcagatggct tgattgtggc
    67321 aacgcctaca ggctcaaccg cctattcact ttctgctggt ggcccgattt taaccccgaa
    67381 tatgaatgca atggcattag tgccgatgca ccctcacact ctttcttctc gtcctttggt
    67441 ggttgatggc gatagtcaaa tttcgcttcg ttttgccaaa tataataccc ctgatttaga
    67501 agtgatttgt gacgggcagg tgaatatgca ctttacgcct gaagatagag tcttggtgcg
    67561 taaaagcccg gataaattac agctgctcca tttaaaagat tatagctact ttactgtatt
    67621 aggttctaaa ttaggctggg caagtaagtt gttttaaaca caagcggtgt tttttctgat
    67681 taaatttgca aatgcaatat gaattgattt catatacatt tcaaaaatat atcattttta
    67741 ttcaataaaa atttgtggtt caataagcta tctgaaaaat aaaggggaca ataaaatgac
    67801 aacaaatttt aaatgccaag tgcatactat tgaatttaat gaagattacc aaccggagca
    67861 aagcacccgt ttaaccacca actttgccaa ccttgcccgt ggcgaaaacc gcaaacagaa
    67921 cctgcaaaat gcgattgcga tgatcaataa ccgttttaac gatttagcga actgggataa
    67981 cccgaatgcg gatcgttatt cggtgaaatt ggatattgtc tcactggata ttgatgtgga
    68041 aggcaaaggc gagtttttcc ctgcgattga gtggctgaaa acctatgtta tcgactacaa
    68101 aaccggcaaa accattgatg gcattatcgg taataacttc tcttcttttg tgcgtgatta
    68161 cgatttcagc gtattgttgt tagatcacaa taaagacaaa ccgcaattta gcgtgccgga
    68221 aggctatggc gagttacacg gcaagctgtt taagcattta ttggcatcgc ctgaatatac
    68281 cgcaaaatat aaaaaaccac cggtgatctg tttaagcgtg gcaaccaccc gtacctacac
    68341 aagaaccact aacgttcacc caattttagg tgtggaatat ctgcaagata gttattcatt
    68401 aactgatgcc tattttgcga aaatgggctt aaaagtacgt tactttatgc cgccaaaatc
    68461 cgttgcaccc tatgcgttct actttgatga gaatgcagat ttactcaatg attacactaa
    68521 cttagagcta atcagcacga ttgccacaat ggaagcgttc caaaaaattt accgtccgga
    68581 aatttacaat gccaattcgg tcgcagggca ggtctataaa ggcagcctga cttaccaaga
    68641 ttattccgtc accaaagtgg aatatgaccg tgaagaacgc acccagttgg cgatcaaaca
    68701 gggtaaatac accgaagagc attttattaa acgttaccaa gcgattttag aggcttgggc
    68761 gacggaggaa taaacgtagg gtgggcgtaa gcccaccata aaatgagata taaccaatgg
    68821 tgggcttacg cccaccctac tttttatagg aaaattcaaa atgagcaaca ttcttctccc
    68881 aacctctacc gccggcagcc taccaaaacc ttcgtggctt gccgagccgg aaaaactgtg
    68941 gtcaccttgg aaatttgaag gcgaggaact acttcaagcc aaacgtgatg ccctccttgt
    69001 ttcccttgcc gaacaaattg cggcagggat tgatattgtt agtgatggcg aacagacccg
    69061 ccagcacttt gtgaccacct ttatcgagca tttggaaggg gtggatttta gcaagcgtga
    69121 aatcgtgcgc attcgtaacc gttacgatgc cagcgtgccg agcgtggtgg gggcagtttc
    69181 ccgcccgaaa tcggtgtttg tggacgatgc caaattcttg cgtgcgcata ccgacaaacc
    69241 aatcaaatgg gcattacccg gcccgatgac aatgatcgac actctctatg acggtcatta
    69301 caaaagccgt gaaaaattgg cgtgggaatt tgccaaaatt ctcaacgaag aagccaaaga
    69361 gttggaagcg gcaggggtgg atattattca atttgacgaa ccggcattta acgtcttctt
    69421 cgatgagctg aacgactggg gcgtggcggc gttagagcgt gcggcggaag gcttaaaatg
    69481 cgaaacggcg gtgcatatct gctacggcta cggcattaaa gccaataccg actggaaaca
    69541 gaccctcggc aacgaatggc gtcaatacga agagagtttc ccgaaactgc aacaatcaaa
    69601 aatcgacatt atctcgttag agtgccaaaa ctcccgtgtg ccaatggact taatcgaatt
    69661 agtgcgtggc aaaaaagtaa tgctgggagc gattgatgtg gctaccaaca ttatcgaaac
    69721 accggaacaa gtggcagata cgttacgcaa agcattacaa tttgtcgatg cggataaact
    69781 ttatccaagc accaactgcg gaatggcacc actttcacgc caagtcgcac aaggtaaatt
    69841 acaggcactc agccaagggg cagcgatatt gcgtgcggaa ttcgcaaaat aattttgcga
    69901 tctgcatcga aaatatcaac tttgcttttt gctcgtttcc agattttctt ccaaaataac
    69961 aggaaatgac ggaggcacta tgctaaatct atttaaaaag ccaattaaga aaagctcctt
    70021 tgaggcttgg gcaaaactaa tggacgatgt ggcaaaagtt gcaattttag caattcctgt
    70081 tattctctat ggagaatatt cactgctaat gaaatttaca cactcaatta ccttatttgc
    70141 agtagtttat tttttgttag caacggcaag attgtttcga gaaacagcag aggagatgta
    70201 aatggacgga ttgacaatcg gattatttct tttgcttgca ttttcatttg ctgcatacca
    70261 atgggcaaag catttaaaag atcataatca ttaattcaat gaataatact aagcggtcat
    70321 atttggtaaa ttttttacaa aatgtgaccg cttgtt
//
LOCUS       DANLUA010000002        56846 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLUA010000002 DANLUA010000000
VERSION     DANLUA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 56846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56846
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            23..1498
                     /gene="nrfA"
                     /locus_tag="PXB44_000077"
     CDS             23..1498
                     /gene="nrfA"
                     /locus_tag="PXB44_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL5280603.1"
                     /translation="MATISILGCLQTALAEEAKTESLTDKAVKHEKLGVKIESANHLF
                     AEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGHFYAVTDVRD
                     ILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGAEIVNSIGCA
                     DCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCANCHVEYYFK
                     NKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEVWSQGTHGKN
                     GVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEKRVKEYKHEV
                     KEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSAAGHGNHFHA
                     PDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLAIGLDIPKEQ
                     AAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
     gene            1517..2173
                     /gene="nrfB"
                     /locus_tag="PXB44_000078"
     CDS             1517..2173
                     /gene="nrfB"
                     /locus_tag="PXB44_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL5280604.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            2173..2850
                     /gene="nrfC"
                     /locus_tag="PXB44_000079"
     CDS             2173..2850
                     /gene="nrfC"
                     /locus_tag="PXB44_000079"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL5280605.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            2850..3797
                     /gene="nrfD"
                     /locus_tag="PXB44_000080"
     CDS             2850..3797
                     /gene="nrfD"
                     /locus_tag="PXB44_000080"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL5280606.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            4067..5971
                     /gene="nrfE"
                     /locus_tag="PXB44_000081"
     CDS             4067..5971
                     /gene="nrfE"
                     /locus_tag="PXB44_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL5280607.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            5968..6501
                     /locus_tag="PXB44_000082"
     CDS             5968..6501
                     /locus_tag="PXB44_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL5280608.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            6491..6943
                     /gene="nrfF"
                     /locus_tag="PXB44_000083"
     CDS             6491..6943
                     /gene="nrfF"
                     /locus_tag="PXB44_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL5280609.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            6930..7691
                     /locus_tag="PXB44_000084"
     CDS             6930..7691
                     /locus_tag="PXB44_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL5280610.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(7743..9137)
                     /locus_tag="PXB44_000085"
     CDS             complement(7743..9137)
                     /locus_tag="PXB44_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL5280611.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            9362..10165
                     /gene="mlaF"
                     /locus_tag="PXB44_000086"
     CDS             9362..10165
                     /gene="mlaF"
                     /locus_tag="PXB44_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL5280612.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            10158..10934
                     /gene="mlaE"
                     /locus_tag="PXB44_000087"
     CDS             10158..10934
                     /gene="mlaE"
                     /locus_tag="PXB44_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL5280613.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            10955..11479
                     /gene="mlaD"
                     /locus_tag="PXB44_000088"
     CDS             10955..11479
                     /gene="mlaD"
                     /locus_tag="PXB44_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL5280614.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            11525..12160
                     /gene="mlaC"
                     /locus_tag="PXB44_000089"
     CDS             11525..12160
                     /gene="mlaC"
                     /locus_tag="PXB44_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL5280615.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            12172..12495
                     /locus_tag="PXB44_000090"
     CDS             12172..12495
                     /locus_tag="PXB44_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL5280616.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            12528..12788
                     /locus_tag="PXB44_000091"
     CDS             12528..12788
                     /locus_tag="PXB44_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5280617.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            12898..14178
                     /gene="murA"
                     /locus_tag="PXB44_000092"
     CDS             12898..14178
                     /gene="murA"
                     /locus_tag="PXB44_000092"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL5280618.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(14244..14822)
                     /gene="ruvC"
                     /locus_tag="PXB44_000093"
     CDS             complement(14244..14822)
                     /gene="ruvC"
                     /locus_tag="PXB44_000093"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL5280619.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(14889..15416)
                     /locus_tag="PXB44_000094"
     CDS             complement(14889..15416)
                     /locus_tag="PXB44_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280620.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            complement(15546..16286)
                     /locus_tag="PXB44_000095"
     CDS             complement(15546..16286)
                     /locus_tag="PXB44_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5280621.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            complement(16299..16739)
                     /gene="nudB"
                     /locus_tag="PXB44_000096"
     CDS             complement(16299..16739)
                     /gene="nudB"
                     /locus_tag="PXB44_000096"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL5280622.1"
                     /translation="MNYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGE
                     TPLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLL
                     PLDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            complement(16875..17750)
                     /locus_tag="PXB44_000097"
     CDS             complement(16875..17750)
                     /locus_tag="PXB44_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5280623.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            17858..18694
                     /locus_tag="PXB44_000098"
     CDS             17858..18694
                     /locus_tag="PXB44_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL5280624.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            complement(18769..21621)
                     /locus_tag="PXB44_000099"
     CDS             complement(18769..21621)
                     /locus_tag="PXB44_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5280625.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(21669..22346)
                     /locus_tag="PXB44_000100"
     CDS             complement(21669..22346)
                     /locus_tag="PXB44_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5280626.1"
                     /translation="MNSIIRKEWFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEG
                     KRGVAFEYHYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGF
                     GAFNSEVYAPDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATT
                     QPKDGKIYVFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVV
                     YIGHEMI"
     gene            22560..22778
                     /locus_tag="PXB44_000101"
     CDS             22560..22778
                     /locus_tag="PXB44_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5280627.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            22788..24758
                     /locus_tag="PXB44_000102"
     CDS             22788..24758
                     /locus_tag="PXB44_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5280628.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQIASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(24938..25426)
                     /locus_tag="PXB44_000103"
     CDS             complement(24938..25426)
                     /locus_tag="PXB44_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280629.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            25458..26399
                     /locus_tag="PXB44_000104"
     CDS             25458..26399
                     /locus_tag="PXB44_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5280630.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            26402..26716
                     /locus_tag="PXB44_000105"
     CDS             26402..26716
                     /locus_tag="PXB44_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280631.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            26728..26964
                     /locus_tag="PXB44_000106"
     CDS             26728..26964
                     /locus_tag="PXB44_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280632.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            26948..27241
                     /locus_tag="PXB44_000107"
     CDS             26948..27241
                     /locus_tag="PXB44_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280633.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            27363..27581
                     /locus_tag="PXB44_000108"
     CDS             27363..27581
                     /locus_tag="PXB44_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5280634.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            27591..27773
                     /locus_tag="PXB44_000109"
     CDS             27591..27773
                     /locus_tag="PXB44_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280635.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            27790..28179
                     /locus_tag="PXB44_000110"
     CDS             27790..28179
                     /locus_tag="PXB44_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280636.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            28228..28515
                     /locus_tag="PXB44_000111"
     CDS             28228..28515
                     /locus_tag="PXB44_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280637.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            28634..29179
                     /locus_tag="PXB44_000112"
     CDS             28634..29179
                     /locus_tag="PXB44_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL5280638.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            29166..29699
                     /locus_tag="PXB44_000113"
     CDS             29166..29699
                     /locus_tag="PXB44_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280639.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            29864..30223
                     /locus_tag="PXB44_000114"
     CDS             29864..30223
                     /locus_tag="PXB44_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5280640.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            30359..30868
                     /locus_tag="PXB44_000115"
     CDS             30359..30868
                     /locus_tag="PXB44_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5280641.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            30871..31140
                     /locus_tag="PXB44_000116"
     CDS             30871..31140
                     /locus_tag="PXB44_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5280642.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            31134..31481
                     /locus_tag="PXB44_000117"
     CDS             31134..31481
                     /locus_tag="PXB44_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5280643.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            31508..31660
                     /locus_tag="PXB44_000118"
     CDS             31508..31660
                     /locus_tag="PXB44_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280644.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            31660..31995
                     /locus_tag="PXB44_000119"
     CDS             31660..31995
                     /locus_tag="PXB44_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL5280645.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            31997..32293
                     /locus_tag="PXB44_000120"
     CDS             31997..32293
                     /locus_tag="PXB44_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280646.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            32316..32888
                     /locus_tag="PXB44_000121"
     CDS             32316..32888
                     /locus_tag="PXB44_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL5280647.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            32888..34444
                     /locus_tag="PXB44_000122"
     CDS             32888..34444
                     /locus_tag="PXB44_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL5280648.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            34563..36017
                     /locus_tag="PXB44_000123"
     CDS             34563..36017
                     /locus_tag="PXB44_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL5280649.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            36010..37287
                     /locus_tag="PXB44_000124"
     CDS             36010..37287
                     /locus_tag="PXB44_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5280650.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRAANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(37489..37833)
                     /locus_tag="PXB44_000125"
     CDS             complement(37489..37833)
                     /locus_tag="PXB44_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280651.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            37897..38361
                     /locus_tag="PXB44_000126"
     CDS             37897..38361
                     /locus_tag="PXB44_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5280652.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            38596..39711
                     /locus_tag="PXB44_000127"
     CDS             38596..39711
                     /locus_tag="PXB44_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL5280653.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            39742..40668
                     /locus_tag="PXB44_000128"
     CDS             39742..40668
                     /locus_tag="PXB44_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL5280654.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            40737..41018
                     /locus_tag="PXB44_000129"
     CDS             40737..41018
                     /locus_tag="PXB44_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280655.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            41018..41452
                     /locus_tag="PXB44_000130"
     CDS             41018..41452
                     /locus_tag="PXB44_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5280656.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            41458..41955
                     /locus_tag="PXB44_000131"
     CDS             41458..41955
                     /locus_tag="PXB44_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL5280657.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            42040..43425
                     /locus_tag="PXB44_000132"
     CDS             42040..43425
                     /locus_tag="PXB44_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL5280658.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            43436..43951
                     /locus_tag="PXB44_000133"
     CDS             43436..43951
                     /locus_tag="PXB44_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5280659.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            44047..44361
                     /locus_tag="PXB44_000134"
     CDS             44047..44361
                     /locus_tag="PXB44_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5280660.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            44358..44510
                     /locus_tag="PXB44_000135"
     CDS             44358..44510
                     /locus_tag="PXB44_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5280661.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(44489..44791)
                     /locus_tag="PXB44_000136"
     CDS             complement(44489..44791)
                     /locus_tag="PXB44_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280662.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            44839..47496
                     /locus_tag="PXB44_000137"
     CDS             44839..47496
                     /locus_tag="PXB44_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5280663.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVTQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            47506..48429
                     /locus_tag="PXB44_000138"
     CDS             47506..48429
                     /locus_tag="PXB44_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5280664.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            48413..48640
                     /locus_tag="PXB44_000139"
     CDS             48413..48640
                     /locus_tag="PXB44_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5280665.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            48633..49697
                     /locus_tag="PXB44_000140"
     CDS             48633..49697
                     /locus_tag="PXB44_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280666.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            49684..50220
                     /locus_tag="PXB44_000141"
     CDS             49684..50220
                     /locus_tag="PXB44_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5280667.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            50275..50637
                     /locus_tag="PXB44_000142"
     CDS             50275..50637
                     /locus_tag="PXB44_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5280668.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            50647..51750
                     /locus_tag="PXB44_000143"
     CDS             50647..51750
                     /locus_tag="PXB44_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5280669.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            51743..52309
                     /locus_tag="PXB44_000144"
     CDS             51743..52309
                     /locus_tag="PXB44_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5280670.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            52319..54598
                     /locus_tag="PXB44_000145"
     CDS             52319..54598
                     /locus_tag="PXB44_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL5280671.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            54599..55201
                     /locus_tag="PXB44_000146"
     CDS             54599..55201
                     /locus_tag="PXB44_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280672.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            55185..55460
                     /locus_tag="PXB44_000147"
     CDS             55185..55460
                     /locus_tag="PXB44_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280673.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            55460..55747
                     /locus_tag="PXB44_000148"
     CDS             55460..55747
                     /locus_tag="PXB44_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280674.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            55757..55921
                     /locus_tag="PXB44_000149"
     CDS             55757..55921
                     /locus_tag="PXB44_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280675.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            55914..56618
                     /locus_tag="PXB44_000150"
     CDS             55914..56618
                     /locus_tag="PXB44_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280676.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
BASE COUNT        17168 a        11403 c        12959 g        15316 t
ORIGIN      
        1 atttttaata aaaagcctag ctgtggcaac aatttcaata ttggggtgtt tacaaactgc
       61 tttagctgaa gaggcgaaaa ctgaatcatt aacagataaa gcagttaagc atgaaaaatt
      121 aggcgtgaaa attgagtctg caaaccacct atttgcagaa aaatatccgc tacaatacga
      181 ttcttggaag tcaactgcaa aatccacaga tcgaggaagt gcgttagagg ctgaccctcg
      241 ttatgttatt ttgtgggcag gctatgcttt tgcgaaagat tataataaac cacgaggtca
      301 cttttatgca gttaccgatg tgcgtgatat tttgcgcacc ggcgcgccta aagatgaaaa
      361 tgatggtcca caacctatgg cttgttggac atgtaaaggc cctgatgttc cacgcttaat
      421 tgaggagaaa ggtgagcgag gatattttga tcctaaatgg gcgaaatatg gtgctgaaat
      481 tgtaaactca ataggttgtg cagactgcca tgacacgaca tcagaagagt ttaaacaagg
      541 taaacctgca ctacgtgttg cacgtcctca cgtattacgt gcattgaata cggttggttg
      601 gaagtttgag gatttagata aacatggtaa acgccctgct gtgtgcgcta actgccatgt
      661 ggagtactat ttcaaaaata aaacagatgt aactttccct tgggataaag gtgtagatgt
      721 agatagcatt gaaaaatact acgatgaaat caattttaca gactggactc atgctttatc
      781 gaaagctccg atgctaaaaa cgcagcaccc agattttgaa gtttggtcac aaggtacaca
      841 tgggaaaaat ggtgtaacct gtattgattg ccatatgcca aaagtgaaag acaaagacgg
      901 caaagtttat actgaccaca aaatcggtaa tccgtttgac caatttgaag cgacttgtaa
      961 aacttgtcat gaacaaagca aagaaacatt ggaaaaacgt gtaaaagagt ataaacacga
     1021 agtaaaagag gcaatgattc gcttagaaga tcaattagtg aaagctcact ttgaagcgaa
     1081 agctgcttgg gaagctggag caactcaaga agagatgaaa gagatcttaa tggcaattcg
     1141 tcacgctcaa tggcgttggg attactctgc ggcaggtcac ggtaaccact tccacgcacc
     1201 tgatgtaatg ttacgtacca tcgcaacagg tatcgaccgt gcagcagatg cccgtgctaa
     1261 attaggtgta gtgttagcga aacacggtgt aacaacgcca gtggcgattc cggacatctc
     1321 aactaaagag aaagcacaac ttgctattgg tttagatatt cctaaagagc aagcagcaaa
     1381 agatgagttc ttaaaaactg tggttcctca atgggaaaaa gaagctcgtg ctaaaggttt
     1441 attacccgca gaagaagcag acaaaccggt agcagcacca aaagcagaag caaaataatg
     1501 tagcttgagg tgaactatga aaaaattaat ctcaaaagca ggacgagcag tcgccctcat
     1561 acaagcggtc gtatttgcag gtttttttgc aatttccagt gcgaatgcgt taatgcctga
     1621 gcagccaaaa cctctttggc aatcagaaac ggctgatgat gcaaaagtag ctcaagatgc
     1681 acgccgtgat ccaaataaat attgtactca atgtcacgaa tcggcaaacg caacaggtaa
     1741 gcctttccat cacgcaggca aacatttcca aaaagatgtg aaaagcccga ataatggaga
     1801 gccactcact tgtataagtt gccacggtaa tatttctgaa agccaccgca aaggggcaaa
     1861 agacgtaatg cgttttaacc cacacggtaa agcaagtaac ccatcacttg aacgttcggt
     1921 aaatgagcaa aatcaagtct gttttgcttg ccatacggca gagaaattgc gtgaaaaatt
     1981 ctgggctcac gacacgcacg caactaaaat tgcttgtacc aactgtcatg aaattcaccc
     2041 ggaaaaagat ccgatgaaag acattcctga aaaagatcgc attaaacttt gtacggattg
     2101 tcataccaaa attcattcag gcgaatttaa aaatcctgaa atgaaagcca cagaagcaaa
     2161 aggaacaaaa taatgacttg cacccgtaga gatttcgtct caggggcagg tgctatcgca
     2221 gttgtggcaa gtacagggct tgccacctcc ataactcttg ctactgagaa agaacaaaag
     2281 aaaatccgct atgcgatggt tcatgatgaa actgcctgta tcggctgtac ggcttgtatg
     2341 gacgcttgtc gcaccaccaa caaagtgccg gaaggggttt ctcgtttaga aattatccgt
     2401 agtgagcctt tcggtgagtt tccgaacgta caatatgagt ttttccgcca atcttgccaa
     2461 cactgtacca atgcaccttg tgtgagtgtt tgcccgaccg gtgcttcatt tatcgatcct
     2521 gaaaccggga ttattgatgt acactcagat ttgtgtgtcg gttgccaata ctgtattgcg
     2581 gtttgcccgt atcgtgtacg ttttattcac cctgagaaaa aatcggcgga taaatgtaac
     2641 ttttgccgag ataccaatct tgcagaaggc aaacagcctg cttgtgtaga ggcttgtccg
     2701 actaaagctt taacctttgg tgatatgaat gatccaagta gcgatatttt ccataaagtg
     2761 aaaaataatc cggtttatcg caccaaaacg gcattaggca cggagccgaa tttatatcac
     2821 attccatttg gtaaaggaga acatagataa tgaatgaata tgtacctttc caaaccccaa
     2881 accttgtttg ggatggcact atcgctattt acttattcct acttggtatt tcatcaggtg
     2941 cggttcagtt agcgattgct taccgtaata gcggtgtcaa aatggaaaaa ccaagccaaa
     3001 actggattat tcgtagtgcg gctattcttg gcacattacc aaccattatc ggcttactgt
     3061 tactgatttt ccacttaacc aaaccttgga cattctggaa attaatgttc aactataact
     3121 ttacctcagt aatgtcgatg ggggtaatgt tattccaact ttatatggca gtattggtgg
     3181 tttggatcgc cataatgttt aaagattgga ttgttcggta tctacctgtg gtctctggtt
     3241 tgatcgattt tgccgaagcg aagctgttaa aaccaattga atttatctta ttcgtattgg
     3301 ctgctgtact tggtgcttac accggtttct tactctcggc gttagtgagt tatccaatgc
     3361 tgaataaccc tgtgttaccg gcattgttct tggcatcagg ggcatcgtca ggtattgcag
     3421 caactttcct agtgatttta ctcgcaggca agttatccgg tgaaagccac gaagtccact
     3481 ttatgcacaa atttgaagtg ccgattatgg taaccgaatt aggcttaatt gtagcgttct
     3541 ttgtgggctt acacttcggt ggagcagata aatcgctcgc tttaacccat gcacttaacg
     3601 gcttctgggg gaatgtgttc tggataggcg taatgttaat cggtattgcg attccgcttg
     3661 ttgccaatat ttttggtagc caccgcttga aacataacgt gaaatttatt atcttaattt
     3721 ccgttttcga cttaatcggt gtactctgct tgcgttactt tatcctttat gcagggcaac
     3781 taacggttgc aagttaggat atactctctc cccttgtggg acaaatgttt acatttgaac
     3841 gttggctttg ccaacggctc cgaaggagcg agcgtagcga gtattgagac cgaaaaatat
     3901 ttcgttcaga aatatttttc agagagaggg agtattgtta tttttagcaa aattatttaa
     3961 ttcccctctc cctgattttt cttctgaacg aagaaaaatc tgtccctctc ccacaagtgg
     4021 agagggcttt ttttacattc acaagcggtc attttttgga ttttttatgc taccggaact
     4081 cggctatttt tcacttttat tggctgcaat tagtgccatt tttcaagttg gttttgcact
     4141 tctaggcgaa gttcgcaagc aatatcattg gctggcattg gctccgcttt tcacttattt
     4201 gcaagctgct tttaccacat tttccttttc taccttagct tatgcttttc taaccgatga
     4261 tttttcggtt atctacgttg ccgctcattc caacagccag ctccccgatt tttttaaatt
     4321 tgccgccact tggggcggac acgaaggctc aatgctgttt tggctcaccg ctcttgtgat
     4381 ttggtctgcg gtattttgca aattttcgca aaaaattgac cgcttgtttg ccaaccgaac
     4441 cttagcgatg ttagggctaa ttgcactagg ttttctactt ttcattctat tggtttccag
     4501 cccatttgaa cgtggctttc caccaccacc tgaagggcga gatctcaacc caatgttgca
     4561 agatattggg ctcatctttc acccgccttt gctctacttg ggctatgtcg gctttgcggt
     4621 gagctttgca atgattgtcg ccgccctcgt ttcaggcgga atggacgctg cgattatgcg
     4681 ttggcttcgt ccgtggacga tgatttcgtg gggatttctc accgccggca ttattttagg
     4741 ggcgtggtgg gcatattacg aactcggctg gggtggctgg tggttttggg atccggtgga
     4801 aaatgcctcc ctaatgcctt ggctacttgg caccgcattg gtgcatagtt taattgtgag
     4861 ccaacagcgt ggcattttct attattggac gattctactt gccattttcg cctttgcttt
     4921 aagcctatta ggcacgttta ttgttcgttc gggtgtactg acctcggttc atgcctttgc
     4981 ggtcgatccc gacagaggaa tggcactgct gatcttattc ttcggcttaa gtttcattgc
     5041 ccttgctctg tttgcattcc gagcgaatct ctggcaaggc aaagtgcgtt tcaacttact
     5101 ttctaaagaa acggcatttt tattgattaa cggcttattt agtgtggctg cttctgtggt
     5161 attgctgggg actttctacc cgatgatctt cacagtgatg aattggggat cgatttcagt
     5221 gggggccccc tatttcaata gcgtattcac gccactagcg ttactgttaa tgatagtaat
     5281 gggctttgcg gtggtgctgg gctggaaaca gatttcaaat aagcagatat tggtaaaact
     5341 ttggttgctg ccgattgcag tcggtttagc gttagggttg atttatgcct ccatctcgca
     5401 aagagagcat tttgctttgc agccgttacc caccatattt atcagccttg cgatctggat
     5461 tattctcact catttgccct attcgcaaaa aatgtggcaa attcgaccgc ttgcgatgcg
     5521 gttagcccat atcggctttg cggtttgcgt gattggggca atgatgaaca gttattacgg
     5581 cgatgaaatc ggcgtgcggt taaagccgaa tcaagcagcg gaacttgccg gttatacctt
     5641 taaatatgaa gattatcacg atctcatcgg tccgaactac accgccgagc aagccgtttt
     5701 ttctatcagc cgagatgaac agttgctggc aaccgtcaca ccggaaagac gctattatga
     5761 tgtaagaacg atgacaatgg cagaagtagg gcttttccac cacaatttag acgatattta
     5821 tattgtgatg ggggataagt tcggcaattt agaatatgcc ttccgtttgc actataagcc
     5881 ttatgtgcaa gccttatggt taggtggtat gatgatggta atggccgccc tgttggcatt
     5941 attcggttat cgtagagaaa acaaaaaatg aaaaaaatcc tgttacttat tccgctcctg
     6001 ttacttgtag ttattgttgg ctttttgact gtgccgttaa tgaataaaga tgcccttgct
     6061 cccactgaag attggcaggg caagccgttt ccagagtttg taggcaagaa cttacttgat
     6121 agtaacgcgc atttaaataa caacagcttg ccgaaagagc cttatattct caatgtgtgg
     6181 gtgagctggt gtacttggtg cattaaagag ttcccgattt tgttggaact aaaacaaaaa
     6241 ggcgtgccga ttgtcggctt aacttatagc gaccgcccaa atgatgccct caacgcattg
     6301 aaaaactggg gaaatccgtt tagtttaatt attgatgatt acgaaaaagg ctttttgatc
     6361 caaaccctga aagtctcttc tgcaccttcc agttatttga ttgataaaca cggtgtagtg
     6421 cgttaccaac aaaagggtta caaccctgat tttgcacagg atttcctacc caaattagag
     6481 gctctcagaa atgagaaata gcataatttc catcggcttt ttgcaaaaaa tccgcaaaat
     6541 attaccgctt gtatttggct tgttgctatt tgtcggcacg gttcaagctg aaatggtgga
     6601 cacctttcaa tttcaaaatg aggcggatcg ggtacgggcg atttcactgg ctcgttcgtt
     6661 gcgatgtttg caatgtcaga accaaaattt ggtcgaatcc aatgcgacgg cggcttacaa
     6721 tatgcgaatt gaagtgtatg aaatggtgaa tcaagggaaa agtaatgagg aaattatccg
     6781 cattatgacc gagcgtttcg gcagttttgt gaattacgat ccaccattca cgccacagac
     6841 atgggtatta tggggcttgc cggttggctt gtttagcctg ttatttctag ctattttgtg
     6901 gcgaactcgc aaaaataaag gcaacaaaaa tgaaaacgcg tgagcaatta agccaagaaa
     6961 actaccaaca agcggttaaa tttgcccaac attttgcaga tgacgtcaga caagagtttg
     7021 agcgtgaggc agagcaacgt taccaatttg aacgaacgca atttgaacag agcgttttgc
     7081 aaaataatgc tcaaaatcga ccgcttgcta aaatcgcaag cctgagttgc gtggcgattt
     7141 tgttgttagc aatgacttac tatgggcttt ccggacgtta tcaagtggtg caatcaggtt
     7201 tgcaacaaca tcaagatttt caacaacaga tgagcgatgc acacggcaca tcgaaaaatg
     7261 aacgctatat tttgagtctg caaaaccagc ttcgagaaaa cccgaataac ggtgatttat
     7321 ggtacgaact agggcaggct tatgcgttaa gcaatgattt tgattcggca ctggtttgct
     7381 acgataatgc cgagaagcta ctcggaaaac gagcggctat tttgggggcg attgccaccg
     7441 ctcgttatta cgataacggg cagaaaatga ccgttgaaat tcaagccatt atcgagcaag
     7501 ccctacagtt ggataaaaat gaaaatgcca gtttactcct gctagcctcc gacagctttt
     7561 taaataacaa ttttcaacaa gctctcgata attggcgtaa agtgttagat agcaataatg
     7621 attctatcaa ccgcagagcg attatccaaa gtatggaaat ggcacgacag atgttaaatt
     7681 cccagcaata acaaaagccg atgatttttg tcatcggctt ttgtttgagt ggatcaaaag
     7741 gtttattgca aaatcacata gaaatttgca ccgtcacgca caatattgag tgcaaccaca
     7801 gatggcttgg tttctaatat tttgcgtaaa tcggcaatgt tttctaccga gtggcggttc
     7861 acgccgatga taatatcgcc ttttttgagg cttcgcattt cagcaatcga gcctttttct
     7921 acgttagaaa tttcaacccc tttaatgcct ttggcattgt agttgttaaa atctgcccct
     7981 tttaatgccg gaatgaggtt tgaagcagta actttggctt cagaatccga ttgtaaggtc
     8041 acttttgctt tggcttcttt gccgtcacgc aggtaagtaa gttcaatctc tttacctaca
     8101 cctgaagtag ctacttttgc tcgtaattcg ctgaaactac ggattttttg accgttgatt
     8161 tcagtaatca catcacctgc tttaaagccg gctttagcag cagcagaatc cggtaagact
     8221 tcgcttacaa atgccccttg ttgggtggaa atattgaact ctttagcaag gtcagcattc
     8281 aattcaccgc ctttaatgcc taacattcca cgttttactt caccaaactc aataatttga
     8341 gtcaccaagc tgtttgccat attacttgga atcgcaaagg cgataccggc attgccacca
     8401 cttggagaaa taatagcggt gttaattccg attaattcgc cgtttaagtt aattaacggg
     8461 ccgccagagt taccttggtt aactgccgca tcggtttgaa tatagttttc atagccttca
     8521 tcggattgac cggttgaacg accaagtgcc gagatgatgc ctgaagtaac agtttgtcct
     8581 aagccaaacg ggttaccgat tgcaactgta aaatcgccca cttgtaattt atcggaatcg
     8641 gcgaatttaa tttcagttaa gttttttgga tcttcaactt gaattaacgc cacatctgaa
     8701 aggggatcag caccaactaa tttagcttta aattcacgtc cgtcttcaag ttttacggta
     8761 attttatcgg cattgtcgat cacgtggtta ttggtaatca cataaccttt ttcggcattg
     8821 atgactgcac ctgatccaac gccacggaaa ttgcgtggag ctgatgagcg gttgccgaat
     8881 aaatcggcgt gaggaccgaa gaaaaattca aattcttccg gaatttcacg actgcggcgg
     8941 ctatcgcctt tggttttacc ttctactgca atactcacca ccgccgggcg aactttttcc
     9001 agcattggtg caaggcttgg cacagcttgc ccatttacgg ctgtgggtaa tgttgcttct
     9061 gcatacgccg gcattgcggc aatggttgag cctaaaatga atgcagttaa aaatgatttt
     9121 ttcattgttt tgttcatatt gtgaatgttc ctcatatcct atgtttttat aatctacgag
     9181 tgaaaacgct cgtattggct aagacaagac ctttcttttt cagttcagcc ggtttgcaaa
     9241 aaatttagca aatctgaccg cttgtaaagg cagatttttg tcagattatt aaaagttttt
     9301 tgacattctg gcaaatttga tagaccataa atatcacttt tattaatttg agtcctattt
     9361 gatgacaaaa acggaaaatt tggttgaagt aaaaaacttg accttcaagc gaggggagcg
     9421 agtgatttac gataacctga atctgcaagt gcagaaaggt aaagtgactg ccattatggg
     9481 gccttctggg attggtaaaa ccaccctgtt gaggctaatc ggtgggcaga ttttacctga
     9541 atcgggcgaa attttatttg atggcagaga tatttgcaaa gccaataaca aagagttgta
     9601 tgaaattcgc caacgaatgg ggatgttgtt tcaatcgggg gcgttattta ccgatatttc
     9661 taccttcgat aatgtggctt tcccgattcg ggagcatacc cgtttacccg aagagctaat
     9721 tcgcaaattg gtgttattga agctcgaggc ggttgggttg cgaggtgcgg caaatttaat
     9781 gccgtccgaa ctttccggcg gtatggcaag acgtgccgcc cttgctcgag cgattgcgtt
     9841 agatccggat ctgattatgt atgatgagcc gtttaccggg caagatccga ttagtatggg
     9901 tgtgattgtc gagctgatta aagagttaaa tcaggcattg aatttgacct ccattgtggt
     9961 gtctcacgat gtgaaagaag tattaagtat tgccgattat gcttatattg tggcagataa
    10021 acgagtgatt gctgaaggta caccggaaat tttacaggca agcgaagatc cgcaagtggt
    10081 acaattctta gccggtaaag ctgatggtcc ggtgcggttc cattatccag caaaagatta
    10141 tacggaggag ctatttaatg gttaatttta ttagctctat cggggcggtg gtgattaatt
    10201 ttatccgtac attagggcgt tccgctttta tgttatgggg agcattaatc ggtaaacctg
    10261 aatttagaaa acataccccg ttactgatta aacagcttta tgtgttagga gtgcaatcgc
    10321 tcttaattat tatgctgtcg ggcttattta tcggaatggt gttaggctta caaggctatg
    10381 tggtattggt cgattttgcc gccgaaagta gcttgggaac tttggtttca ctctcgcttt
    10441 tacgagaact tggtcctgta gttactgcat tattatttgc agggcgagcc ggttcggcat
    10501 tgaccgcaga aatcgggctg atgaaagcca cagagcaact ttccagtctt gaaatgatgg
    10561 cggttgatcc gcttagacgt atcatcgctc ctcgcttttg ggcaggcgtg atttcaatgc
    10621 cggtgttagc tgctattttt accgctatcg gaatttgggg cggctcatta gtcggggtag
    10681 attggaaagg cgttgatggt ggcagttttt ggtcggtaat gcaaaactcg gtaaatacca
    10741 gcgatttaat taacggcttt attaaaagtg tgatttttgc ctttgcggtg gtctggattg
    10801 ccttatttaa cggctacgat tgcgtaccga cttcagaagg aatcagccaa gcgaccacaa
    10861 gaacggtggt aaatgcttca ctggtgattt taggattgga ttttatttta actgcgataa
    10921 tgttcggcgg ttgatttttt gaaaggaaca gacaatgcgt caatcaatta aatatgaatt
    10981 ttgggtaggc ttatttgtac tacttgggct tgcagctctt gtctttttag gcttaagagt
    11041 agcaaatgtg cagggcttct caaatgagaa aacttacacc ctgtatgcaa cttttgataa
    11101 tattggtgga ctaaaggttc gtgcgcctat taaagttggc ggtgtggtag ttggccgagt
    11161 ggctgatatt tcacttgata acaaaaccta caccccgaaa gtggcattag cggtaaatga
    11221 aagttttaat caaattcccg ataccagctc gctctcaatt aaaacctcag gcttattagg
    11281 cgagcaatac gttgcgttaa atgttggctt cgtaatcgaa ggtgaaactg caatgatgaa
    11341 agaaggtgat acttttgtag ataccaattc tgcaatggtg ttagaagatt taatcggtca
    11401 gttcttatat ggtgataaaa agtcagataa aactgaagat gaaaaagcaa gtgattctgc
    11461 tgaaccaaaa gcagagtaat taagtacaac aacctctaat ttagagtttt ggagagcatc
    11521 aataatgtta aaaaacatta aaaaaattat cgtaacaggc ttagtggcag tttctgcatt
    11581 attttcaacc accgcatttg ctgaaactag cccttatgta ttaatgcaac aaaccgcaga
    11641 taaattattt ggcgatatta aagcaaatca agcgaaaatt aaatcaaacc ctgaatattt
    11701 gcgtaccatt gtgcgtaacg atttaatgcc acacgttcac gtgaaatatg caggccaatt
    11761 agtgttaggg aaaaacttat cggctgcgac tgatgcacaa aaagaggcat tctttaatgc
    11821 ttttggtcaa tttgttgagc agtcttatgc tcaggtgtta acccagtatc aagatcaaag
    11881 tgtgcaaatt gaaagcccga aatcggtgga tggtaaatct atcgtaagca ttcgtgtaaa
    11941 cgtgtcaggt aaaggtgctg cacagccgat caaattagat tttaaatggc gtaaaaacag
    12001 caaaacaggt gaatggcaag catacgatat ggccgctgaa ggagtaagta tggttgcaac
    12061 caagcaaaac gaatgggcgg gtgtgattcg tcaaaaaggg attgatgcct taattgcaca
    12121 agtggcacaa tctgcaaaac agcctatcac tttaaaataa gtagtttagt tatgcctcaa
    12181 aaaacattac aatgggacat tcagcaaaat aacgaaagtc tttttgtgaa actatcaggc
    12241 gaattaactc gtaacacgtt gctcccttta tggaagcaac gtgcttcttt tttatctcca
    12301 acagggaaac aaagcctata ttgggattta aaagaaatca ctcggattga ttctgccggt
    12361 tttaccttac ttgctgaatt actgaatcat taccataaaa tcataccaaa cagtttgatt
    12421 aatacgccag attctgtcag aactttggct gcattatacg atttagacgg ttggtttgag
    12481 caatttatta tttgagtgag ttaccaatca tattttagga agtcagaatg aatccatcac
    12541 aaattgaaga aattttaaaa caagcattat ctgatgcaac agaagtccac gctcaaggcg
    12601 agaatgccca ctttggcgtt attgtagtaa gtgacagcat tgcttctctt tcaaaagtaa
    12661 aacaacagca agctgtttat gcccctttag ctgagtattt cactactaat gcgattcacg
    12721 ctttaaccat taaagtattc agcgtggaaa aatggaaaac tgagcgtttg ctaaatatgg
    12781 ttggatagat gaaatgtagg ggcgaatcac tattcgccca aactcttttg taaacgttgg
    12841 aaaattgggc gaaaaatttt tcgcccctac aaattattat tttttaggat ctcccaaatg
    12901 gaaaaatttc gtgtgtatgg tccattttct ttaaatggta ctgtcgatat ttcaggtgcg
    12961 aaaaatgccg cactccctat tttatttgcc gctattttgg ctgaaaaaac agttacgtta
    13021 aaaaatgtgc ctgatttaaa agatgtggac accactttta aaatcttacg ccaattaggg
    13081 gtaacggttg aaaaaggtag cgataaaggc gtggtacata ttgatgcgag taagatcaat
    13141 aattacgttg caccttacga attagtgaaa acaatgcgtg cttcaatttg ggcgttagct
    13201 ccattagttg cccggttcca acttgggcaa gtttctttac cgggcggttg tactatcggg
    13261 gcaagaccgg tagatatgca catttccggt cttgaaaaaa tgggggcgaa aatcgagctt
    13321 gatgaaggct atgtcaaagc aacttcaaac ggtcgtttaa acggtgcaag aatctatatg
    13381 gataaagtga gcgtaggagc aactttatcg gtgatgatgg caggtactct agcaaaaggt
    13441 actactgtta ttgaaaatgc tgctcgtgag cctgaaattg tagatactgc cgatttctta
    13501 aatgcgatgg gagctaaaat ttctggtgca ggtaccgata tgattaccat tgaaggggtt
    13561 gagcatttag gcggttgcga acacagcgta gtgccggatc gtattgaaac cggtacattt
    13621 ttagtggcag cggctatttc cggtggtcga attacttgcc gtaacaccaa agcagacact
    13681 cttgatgcag tgatcgaaaa attacgtgaa gctggaatgc aggttgatgt gacggaaaat
    13741 actatcacgc ttgattctct aggtcgtcgc ccgaaagcag tgaacattcg cactatgcca
    13801 cacccaggtt tcccaactga tatgcaagct caatttacct tattaaatac agttgcagaa
    13861 ggcacaagcc gtattacaga aacgattttt gaaaaccgtt ttatgcatat tcctgagctg
    13921 attcgtatgg gtgccaaagc ggaaattgaa ggtaatattg caatttgcta tggtgtagag
    13981 cagttaaaat cagcagaagt aatggcaact gacttgcgtg cttccatcag cttagtgttg
    14041 gcaggctgta ttgcaacggg tgaaacgatt gtggatagaa tttaccacat agaccgtggc
    14101 tatgaacaca tcgaagaaaa attacgtcag attggggcga gaattgaacg tttctcagct
    14161 ccttttgaag gcgagtaatt gtaaaatttt ctcaaaaaat gaccgcttga aacattagtt
    14221 ccaagcggtc attttatatt gagttagcct tttaacctaa accttccccg gctataacgg
    14281 gttttcatta gtgctaaaat ttgctctttc tcactactca cattctgctg gctttgctta
    14341 gcaacaatca gtgaatgttg gattgaatga gcgtgggtaa tcgcaatggc taatgcatca
    14401 gccgcatcgg cttgtggttt ggcggagagt ttaagcatac gggttaccat atcttgcact
    14461 tgtactttat cagcagaacc aattcccgta acggtttgtt tgactaatcg ggcggcatat
    14521 tcaaacacag gtaaatcgtg attaaccgct gctacaatcg ctgttcctcg agcctgcccg
    14581 agctttaagg cagaatcagg gttttttgcc ataaatacct gctctatggc gaacatattt
    14641 ggttgaaatt gagtgatgat ttcactcacg cctgcataaa tccgttttaa gcgggtaggc
    14701 agatcttcag ccgtggttcg aatagcccca cttcctaaat attccagctg tcgcccgttt
    14761 tggcgaatca caccgtaacc tgttaccctt gagccggggt caattcctaa aataatagcc
    14821 atctttgtgc aataaaataa gcgagaagat attatcttct cgcttatgag tagtaaaatc
    14881 aagaaagatt atttatttaa ttttgaggtt aatgctttat ctacatcaca taatttagca
    14941 acgatggtat ctgttttagc accatacttg gttagcatac ctgactggcc attagatgat
    15001 aaggtaaagc ctgtgtctaa atcccaagaa tatttatccg ttttgaatga agtagaatct
    15061 tggtttgagg catcacgagt taatgttggg atcttttgtg ttttaccgcc gctttggact
    15121 aatagattta ctgcttttgc ctcttctcct tggaagacat aattcgcagt tacacgttta
    15181 ccactttggc aacgatatac aattgatttt gcaccatcag ttgctttttc tacttttact
    15241 gcaccacttg cacgaggtgc attacctgca atttcattca cttgctgtgt tgcttgttca
    15301 gccactttag tcacagcgtc ctgagcttgc tgaggagcag attgtgtgtt tgcattagag
    15361 caagcggcta atactaaagt tgctgctagg gcagggacaa atttcactaa tttcatagac
    15421 tctccttaaa atgttatagt aaagtagtag catataaact acattaggtt agtcagtaat
    15481 ataggaaaaa agttcataaa aaataagcat aacctaaatt atgcttattt tgcgtgattt
    15541 ttttattaca gtaatgcagc gacttcgtca ctgatttcac cattgtgata aacgttttgt
    15601 acatcatcac aatcttcaag gcggttgatt aaatcaagaa gttttggtgc agtttctgcg
    15661 tcaagttcaa cggtagttga tggaatcatt gttacttctg cattctcaat tttaaagcct
    15721 gcagcctcaa tgccgtcttt cactgagcct aaatcttccc aagcggtata aatttcaaag
    15781 ctaccatctt cttgtggttg aatatcgtcc gcaccggcct caatagcagc ttcagttaag
    15841 gcatcttcat cggcatttgc cactaaaatt aagccttttt tgctaaataa ataacctacc
    15901 gaaccctcag tacctaggtt accgccacat tttgtgaaac ttggacgaac ttgggaaatc
    15961 gtacggttag cgttatcact taaacattcc accatcactg ctgtaccacc cgggccgtaa
    16021 ccttcgtaga ttttggtttc catattggta tcatcgccac cgcctacacc acgctcgatt
    16081 gcacggttga tggtatcacg ggtcatattg ctggataacg ctttatccac cgccgcacgc
    16141 aaacgagggt tggaagaggt atcaccaccg ccgagtttgg cggcagtcac caattcacga
    16201 attaatttag taaaaatttt accgcgttgt gcatcttgtg ccgctttacg gtgtttaata
    16261 tttgcccact tactatggcc tgccattctg ttgtttcctt attttgatgt taaatattct
    16321 tgtattgctt gggcattgtt gggagattta gtcagtcgaa ccgcatcttc cactgaaacc
    16381 caacgatatg ccaaatgttc ggttaaaata ggctcttgct cactatcaag tggcagcaaa
    16441 aaccaatgtt cgacacaatg tgtgatatta ggtgcgtatt tataccgaaa atgcgggaaa
    16501 atttcaaatt ctatctgctt ttggcaatca acaagcggtt gtttttgctc aaaagtttgc
    16561 aaaccggttt cttcgctcac ttcccgttct gctgcttcca gcggtgtttc tccaatattg
    16621 atcgaaccgg tcaccgattg ccaaaaatca ggatcgtcct tgcgttgtag catcaatact
    16681 cgttgggtat ttttagcata aatcaccaca aggatactat gaggattttt atagttcatt
    16741 ttattactaa gatgtgaaat ttaatttttt taggtatcgt agggtgggcg taagcccacg
    16801 gataaatgat ttattggttt tgcgtgggct tacgcccacc ctacaaagct agataaaaaa
    16861 cgtaaaaatc cgatctagcc cagcaccacc aagattgcgg caatcactac taaaatcagc
    16921 cctataaaat cttgaatttt aagccgttct ttaaacacaa aataggaaat accgagaata
    16981 aagaaaattt ccacttgtcc aagcgttttt acaaaggcaa cgtggttcag gctcattgca
    17041 ttgaaccaac cgaatgagcc gagaaatgaa aacaggctgg tcaacatacc taattttgga
    17101 aaacgaaaga atttaatcag tgtctctttt tggagaagag ctaggtatat aaccaatatc
    17161 actgcctcaa agctaatcac gctaaaaagt acccacgccg ctgaaagcaa atagtggctg
    17221 ttgagttgca ggcttgcctc tcgcacccaa agtgatgtga gggcgaaaca taaaccgctg
    17281 gctatgccga gaaagagtgt tttccacgaa agttggcgta gattaccgct gccggtcatt
    17341 aagaaaatcg ccactccacc tagtaccacg ccaagccagc caatgccact gatggttaca
    17401 ccaaaaaata acacgcccag taaagccgcc catactgcct cgcttttcgc cagcccgaca
    17461 cctattgcat aattttttaa gtgaaaaagt tgcaccatta gcgaagttgc cagaatttga
    17521 gccaacccag ccccgatagc atatacaaaa aacagcggtg gaaaatgggg aatgttgagg
    17581 ttatcgtcat attggtataa aaaatagaga taaccgcttg ccaacggcac agcatagaaa
    17641 aagcgggcga gcgttacccc caatacaggc acattgacgc ttaattgctt ttgaaaggca
    17701 ttgcgaccgg cttgagccac ggctgccagc agagtaaacc aaatccacat aatgatgatg
    17761 aaataagaaa agatagccaa atcttactct tttattctca gtttgcaagc ggttaaaaaa
    17821 ccattaaaat ttgcaaaaaa ttgaaagaat aaacacaatg ctccctcata tttatattgg
    17881 caccggcggc tatgccgaca cagatttaat cggcacgctt tacccattcg gcactgcaaa
    17941 agaggatttt ctctttgtct atagccaaca ttacgacacg ctggaaatta acagcacatt
    18001 ccacgccccc atcgggcaga aagcctttga aggaatggtg cagaaagcgg aaggtcggct
    18061 gaatttttcg gtgaaactgc atcaagattt cagccatcaa cgcacggcga ctttagatca
    18121 agcacaatta tttcttaatg cgttagctcc gttcaccgct gaagggttat tggcaaattt
    18181 atttgttcag ttcccaactc aatttgaacg tatgccactt aaccgccgtt accttgccga
    18241 gttgtgccaa tggtttgcgg attatccgtt agcgattgaa tttcgccacc caagctggca
    18301 tattccggcg gtgtttgata cctttgcgaa aagctctaat ctgatttggt gtaatgtgga
    18361 ttacccacaa aatatcggct taccggcatt tcactttttt gccaaccaac gtactgctta
    18421 tttacgctta catggtcgta acccgaattg gtggaaaggg cagagtgcgg cagaacgcca
    18481 cgattaccgc tattcagacg aggaattaaa aggcttggca gaattgcttt ttagccgcag
    18541 aacggaattt gacaggctct atctctactt tgaaaacacc actaaaagcc acgcctttta
    18601 taatattcaa accctaaaag gctttttggc ggaaaaagga tttcaaatca aacctgtgcc
    18661 ggagaatttg cttgggcagc agagtttgtt ttgatgggta atttacattg ttgaggagta
    18721 aataaagggc catgccattt agtaatggat agcccttaat ctcaatattt accattcata
    18781 tgtaactttg aacgagatat aacgctgttt atttagcgta ttccccgtta agaaacctgc
    18841 ctgaattcct gcgtttatat tgcgataatt agcggatata cctgcttctt gtttaaaata
    18901 acgccccatt tgctgctcta tttgtaaatt attgaaacgt gttcttaatt tcccatggct
    18961 tgcatcaaca aaatagctac ctatttccgg ttttatttgc aggccattta gttcaagcgt
    19021 tttattaatt gacagaccag cttgaagagc gagaagatca acagcgtttg tttcgatttt
    19081 tgcatctaca agttgattac ctgctgaaga taaatgatga taacgggcgc taaatgatgg
    19141 gattatattc acgtttgcag attcccatgc tttaccgata ctaagccctg ttacaaatac
    19201 tgaacgatct aattttactg tatttgcttg ataagttaag cgattgcttg ctttcccaaa
    19261 accggtatct atcgcaacaa aaacaccatt ttgccatgtt ttcttcgcat acattgatag
    19321 cattgttaag cgacctttac cgctataata atggtcaaaa ttatttctcg cttttgattg
    19381 agataatatc ataccaagtg tgacctgatt atctaaggct ttctctaatc ctagatggat
    19441 aacgttcgca tcttggttaa atccatatcc ggcagagcga tgctctgtcg tttgataatc
    19501 tgttttagac catactttcc aattttgggt cccttccgaa tgaattacat gatctaatgc
    19561 tgtagttaca cctaatacag cattagtttg cgcagttaac tcggatgcga tcaaatgtgc
    19621 atttccattg tatttaggta atggctcagc ggtttcaccc gatccttttt ctgaatgctc
    19681 aatgtctcct gtgtattcag gtaacggatc agcagtttca cccggccctt tttccgaatg
    19741 ctcaatgtct cctgtgtatt caggtaacgg atcagcagtt tcacccggcc ctttttcaga
    19801 atactcaata tccccggtgt attcaggtaa tgggtcagcg gtttcacttg gccctttttg
    19861 agtaatttta ggttcttcct ctttctctgg attgaggtaa tacacattcc cttctttgag
    19921 taaatggtag cgatattcgc ctgagtgcac ttcatttgct aaattaagag aaaagtcttc
    19981 ttcatttgtt gtttcaaata atttaatctt ttgggctgaa tgggcgctac catttgtgct
    20041 aatttctatc gaaaattgac cgcttgcggt gtctttaatt gtgactatat cgctcgctgt
    20101 tttcgtcaga tcagcgacaa atttaaatat gccgttccct gtgagggtat tagcggtaag
    20161 cgtattctgt ttttgggtcc cttccgaatg aattacatga tctaatactg tagttacacc
    20221 taatacagca ttagtttgcg cagttaactc ggatgcgatc aaatgtgcat ttccattgta
    20281 tttaggtaat ggctcagcgg tttcacccga tcctttttct gaatgctcaa tgtcgcctgt
    20341 gtattcaggt aacggatcag cagtttcacc cggccctttt tcagaatgct caatatcccc
    20401 ggtgtattca ggtaatgggt cagcggtttc acttggccct ttttgagtaa ttttaggttc
    20461 ttcctctttc tctggattga ggtaatacac attcccttct ttgagtaaat ggtagcgata
    20521 ttcgcctgag cgcacttcat ttgctaaatt aagagaaaag tcttcttcat ttgttgtttc
    20581 aaataattta atcttttggg ctgaatgggc gctaccattt gtgctaattt ctatcgaaaa
    20641 ttgaccgctt gcggtgtctt taattgtgac tatatcgctc gctgttttcg tcagatcagc
    20701 gacaaattta aagatgccgt tccctgtgag ggtattagcg gtaagcgtat tctgttttgt
    20761 tgcagttgga gtgtttaaat ggatttggct accatttgcg acattcaaga catctacaac
    20821 agtgttggtt aaggttgtcc ataccccaga aagattaacg gttgaaccat tggcgccatt
    20881 aaatattcct gttgtactca agccggttaa attagcaata cttccatcat taagttgaac
    20941 cttagtggac tcctgagctg taattgagcc agttaagtct gctttactgc ctaacgaaaa
    21001 tgttgaattg tcagctaaag agacattgcc agccactttg gttctctccc agctgtttaa
    21061 atcagtctct gataaagtag cgttaatatt acaggttgct acgcctgtgc ggtcagagcg
    21121 aatacatgct tgtgaggtac cttgggtaaa accaatttga gcggtagcat tatctgttaa
    21181 attaaaatta ccgttaatat tggcaacatt acggctgatt tcaagcttgc cattgtcttt
    21241 agctgcaaag attgttgcgt tgaacgatcg gttgatccag tcgccttcaa tcaggacttc
    21301 tttattcgct actttatcat aggcatgagg tgttggacgg cccgataaaa tgatttcgct
    21361 tgtaccttct gcagaaagcg taccgttgag gttcgttccc ccagtgataa gcaatttgcc
    21421 gtttgctact tcattactgt atagaaccat taaaactaag aaataaaaga ataataggaa
    21481 tttggcggaa tttttcggga ataagtgaaa cagaagtatt gaaaacaggg gctttgcggt
    21541 gcattgcccc tttgttttat gtagaaaaaa gtaaaaataa tgaattgcga aaaaatggct
    21601 aaaactaacc gcttgtacca ttaaaacaac gaaaaagtaa aaatgattta agtagcttta
    21661 aatggcgttt aaatcatctc gtgtccaatg tacacaacct gcccgataac ctcaaaatct
    21721 aacgtttcat caaaaatcac atcaataggg ctgtatagct ctttattatc gctgattaag
    21781 cgaataccgc ccataatgcc ttgcacacgt ttaacccaaa gctgctcgcc ttggcggaac
    21841 acataaattt taccgtcttt aggctgggtg gtggctcggt ttaccaatag catatcgcca
    21901 tcactgatgg ttgggtacat tgaatcacct gaagcggtaa taaatgcaag atagctcaaa
    21961 tagaagccac gcacatctag ccatcgttta cttagcccaa tatagtcatc aggggcatac
    22021 acctcgctat tgaaagcacc aaagcctgct gaagcctgta cattataaaa cggcacacgt
    22081 tccatttcat ctgcagtttg ggctgccact aatgttctag gctcttcttt tactgccgtt
    22141 tgagcaaagc ctaactcttg ctgcacgctt attgggagag agctgtagtg gtattcaaaa
    22201 gcaacccccc tttttccctc tatttgtctt ttgagccaat tatttttggt agctcttctt
    22261 gttacatttg atggttgttc aggtaaattt cccattccag ctacttcaga tgccgtaaac
    22321 cattcctttc taattataga attcataaaa ccccttttga attcaagtgg aattcaaaaa
    22381 aatttacaaa caaattctct tgaaaatcaa ttagttaatt aaataaaacc gaaaaacact
    22441 aaattctttt gaattctctt attgaattca tttggaattc actatattat tcgttctgta
    22501 gttaaatgat ttacatcatt taagtagttt ctaaaactaa ctttcaagga tctcacaaaa
    22561 tggcaagaaa taaaagagtt cgagatatga gtaatttcga aattcgtggt gagctgatga
    22621 aaatcggtaa gtccttatct cagttaggta ttgagaatgg attagcaaaa accacagttc
    22681 gtaatgcgtt agataagcca tatcccaaag gggagcaaat tattgcaaaa gcgattggta
    22741 aaacaccgca ggaaatttgg ccctctcgtt acgaataagg agcttttatg aaggaatggt
    22801 ttttagccaa agaaatcgca ggattatctg gagttccaga attaccgaat agcgtttcaa
    22861 gattggcaac caaagaagga tggcaaaaac gacaaattca aggtgtaaga ggtgttactt
    22921 acgaatacca cctcaccagc ctacccattg aaacccaaca acaacttaga ctaaacgccg
    22981 cccttgcggt aataccgcaa gcggcagaac tacaacccaa acgagacgac cccgcactta
    23041 tcgcaaggct caataatgcc accgataaag gcagggataa agcaaaaggc aaggcagaag
    23101 cctgtatgca gttgcaagcc tttctcgacc aagggtttag ttatacgcag gcagaagcgg
    23161 gagcggcaac cgctaaaaat gtgtcgcaag gttctttaaa aaattggtac tacaaagtga
    23221 aaggtcaccc cgtccattta tggcaggcga tcttaatttc cgaaagcggt aggagtaaaa
    23281 agccgcagct gaaagccaag attactgaag aagcgtggga ctgctttttg gcggattacc
    23341 tccgccctga aaagccggat ttacgggcaa gttaccgccg aacccaagcc atcgccaagc
    23401 aatacggctg gcaaatagca agtttacaga ctttccaacg ccgagtgttg gcggaagtgc
    23461 cttacgaggt gattttgctc aaacgggaag gagcaaatgc ggttgccaag ttagtgccgg
    23521 cactacaacg cacggtgaaa gatattctcg caggagaatg gattaacggc gatggctatc
    23581 aacacaatgt gtttgtgaaa tggcacaccg gcgagattgt ccgccctaaa acgtggtttt
    23641 ggcaagatgt tcgcacccgc aagattttag gttatcgaac atcgatttcg gagaatactg
    23701 acagcatacg ccacgcatta atggatgtga tttttaacgt aggcataccg aaaacgctca
    23761 cgctggataa cacccgagca gcagcgaata aggcaatgac cggtggtatt gaaaaccgct
    23821 accgctttaa acataccgag ttagacccga aaggcattat gccgatttta gggattgatg
    23881 tgcattttac ctcaattctt tatggtgaag gacacgggca agccaagccg attgaacgtg
    23941 ctttcgggcg aggcggaatt ggcgagaaaa tagataaacg ccctgaatta agtggtttct
    24001 atacgggcag aaatgcacaa gaaacccctg ataactataa cggcggtaaa gacggtgtgg
    24061 attacaacac attcttgaaa gccatagcag cagggataga agagtacaac tctcaaaccg
    24121 aacgccatac cgaaatgtgc cgaggagaat tgagttttaa ccaagtttgg gagcgggatt
    24181 accacccaag caacgtaaga caagccagcc ctgaacagtt gcggttactg ttcttacagg
    24241 cagaaacggt cagcattaaa cgcaacggca gttttacgct gaaagcagcc ggcaaacttt
    24301 acggcttaac taacgtttac tgggcagaaa gcctgatagg gataacggat aagaaagtag
    24361 tggcacggtt cgaccccgat gacttacacg gcaacgtgta tgtgtacaac ctagaggggc
    24421 aattcttagc cgaagcggtt tgccgagagg cgaaaggatt tggtgatacc agtgcaagcc
    24481 gtgagcaagg tcgcttatat cagaaagtag tgaaaagtgc caaagctcaa gccgaagccc
    24541 ttgaattact ggaagctcac gaactggcaa gcctcgcccc acaagtggac gtgcctgagc
    24601 cgatagagaa gaaggtaaaa gaggtgctgg tgaaagaaga ttttatcgtg gatttcaaca
    24661 ctgtgcgtaa agcaacggtg gtggaagaaa ccgaagagat cagtattttt gatgaagtgt
    24721 tactgaaaca agagaaacaa cttcgcatag tggaataact agcggtcagt ttttgcaaaa
    24781 aatttgcaaa tttttctaaa aaaacgaccg cttgaaatga gatttaaaca gggtttaaac
    24841 gcctttaagg agtaaaaaat gacattaaca gggcaagctt accgagtgat tttcaaggaa
    24901 ctcaaagagc aacaagagag tgaatttgaa caggctttca agaaagggat tgccaaaatt
    24961 cagcagaatc aaactcagga atccattctg gtaaaccgtg atatgtttca agctgaaaag
    25021 ctaacactgc tttcacaaaa gttggaagcg gttgagcaat tacttcgcct tgaattaaat
    25081 cagccgaatt ttcaagcaaa acttgaagac tacgctgacg cattacgaac aaccatttta
    25141 agtaaagtag ccaaataaga gctttagact cttgaaggct taacatctga gccacttttt
    25201 ttactgtcgg cattataccg tcaagatcat caatagaagt taaatgatga aaagaggaat
    25261 ggtattcagc taaacaaaaa ttgattgtac taagtttaaa caaagtaatg caatttttca
    25321 tcacgatggc atttgctcgt tttaacttaa aaccagagaa ggttgcacga gcagaatcaa
    25381 gggctatttg atgaatttca tcatctgtta aagaacgaaa gagcataaaa acctccaaac
    25441 aaaaacagga caccattatg acattaatcg accaaattaa acaacacatc gccgaaagcg
    25501 gcagttctca ggggaaagtt gccaaagaag ccgggattaa tgccggggcg ttatcggctt
    25561 acctgaatga aaattacaaa ggcaataatg aagaacttga agcaaagctg attgcttatt
    25621 tagagcgtat tgaggcgaag aaaagagagt ttgtcgaagc tccgagtttt attgaaacca
    25681 aaaccgcaaa ccaaattttc ggttcgttac gatttgctca aaatactggc gtgcttgcca
    25741 ttattcacgg tgcgagtggc gtgggtaaaa cacaagcggc tagagagtat cgtaaacgct
    25801 atgcgaatgt atggttggtt accgcaagcc cttctcgttc ctcattaagt gaggtgttat
    25861 atgaaattgc tcttgaactt ggaatgaacg atgcaccacg ccgcaaaggg acattggctc
    25921 gtttaattgt tcgcaaaatg aaaggcacag caggtttatt aattattgat gaagcagacc
    25981 atttacctta cgaagcctta gaagaattaa ggctgttaca agaagaagta gtgaatgaaa
    26041 gtgacatcgg agctggtgtt ggcttagtgc tgattggcaa tgacaaagtc tacacccgaa
    26101 tgaaaggcgg aattaaccct gctcacgaat atgggcgact ttggtcacgc agtgccaaac
    26161 gcaccagtat tcagaaaacc aaaaaagaag atacccaagc ggtagccaaa gcgtgggggt
    26221 tgagtgaaaa ccaagaagca ctcaaggtaa tgcaagccat tactgaaact ggtggcggat
    26281 tacggattct gacacacacc ttacgattag cagcgatggt agcaaaagac cgaatgattc
    26341 ccttaactgc cgatttaatt cagctcgcac gtaaagattt actctcaaca ggagattaac
    26401 aatgagaacc ccaaaaccca aacacagttt taaccgcacc aaccaagcag cgtgccgttt
    26461 tttaaaacaa acccaaaagg caattatccg cttaaacagt cttggcttta cggtgctgaa
    26521 tattgatttt acccgtatta agccccgcat tgaggtggaa atcggcaata acaagcacat
    26581 tgctcaaggc ttgatttatg agggtaaagc ctaccgctac agcttcggta aaagcgaaga
    26641 tttaggcaga tgggaaggct actacaccat gctggaaggt atcagagtgt ggtggcgaca
    26701 agcagcaatt cactaggaga aaaacagatg aaaaccttag cactattaac cgccctttta
    26761 gggcttgtag ggtgtgattt agtccaaagc aaacaagcta aaaattacat cggcaacaat
    26821 ttaactgaaa tctgtataga taacgtggtg tacctgattt attccggtga taaaaaaggc
    26881 ggaattaccc cgaaagtaaa taaagatttt tacccttata cctgtacaaa caaagaggaa
    26941 agacaaaatg gcaaaacccg atgaaacacc cattacaaaa gcagaatgtc aatctcagct
    27001 tgcagagttg ggggtgcaat ataaaaaact gccaatggca attacaagac atatttgtaa
    27061 tgccacaacc aatatacacg gcaaagttat aaaggtcagt gtggtagaga gagttggata
    27121 tggcgtccag attaccgcac aaggtaacga gaaatcttgc ttagtaacct atgaagcaat
    27181 gttaggtatg gcagaagcaa tgggattatt tgatgaagtt aaggagcaaa ataatgacta
    27241 aaaaaacaac cagagtaaaa agtgcaaccc aaagtgcgat ttatcaaagc cgagatgaag
    27301 tgcaagtggc tataaaaatc attggtgata aacagcgtga attgcaacgt ttagctacgg
    27361 caatgaatga tgaattagct gctattagtg ccagttatgc tcaacataac cgctaccaac
    27421 attatgccga gaaagcggct gaagcagaac gcaaaggcaa ctataaggaa gctcaagacc
    27481 attgggaagt tgcaaaatta tcagcaaaaa agaccgctaa ccgagactgg gcggaacaac
    27541 gagcggaatt ttgtaaacgt atgcacaata aaccgtttta gggggaagtg atgacagaaa
    27601 ccgtaaaagc acaacttaac agccaattaa atgaggcaat tatccagttg atacaagctc
    27661 aaaagtattt aaaccaagat gatgccattc gcagtggagt gtatatcggt acggtgcagg
    27721 atttattgcc aaaggtgcat ttaaagttat taacggcaaa tcgtaaacat tgatagagat
    27781 gaggtaaaaa tggctattac agaagaacaa tggaaagaaa ttaagcaaaa gctagatagt
    27841 atcataggga gagttaaatt tagatacaaa gagcatttac tcacggtaga tgttgtacaa
    27901 attaagcgat ctttaaaatt ggctgtgtat gttgatgggg aaattgatgg agcttggaca
    27961 aaagaaggac acgaaattcg cccttattta gaagaagttt ggtatcgcaa agaacgccct
    28021 ttttttaatg ctaaggagaa gaaagagtat agaggtttaa tgagcaaaaa aaaactgaat
    28081 gaaaaaatag taacctactc acctatgttt ccatcaccaa ctgcactgac tcgtcaatat
    28141 aagaaactag atggcttaga gttgattgaa gtgatttaaa acccatttac agcccattta
    28201 accccgagtt atttaaagga caacaaaatg aataaaaacg aattttttaa gaaataccac
    28261 ctagagggtt cactagctat tttggatttg gatgcaaatg acgatcaaag ccaattctgt
    28321 taccaagcat tgttggagta tagagaactc ccgttagaca catttcttgc agagtactca
    28381 gatgatgagg atatgacaga gtcagagttt attgagttaa tgcttgatga attagagtgg
    28441 attatccgca gagctaagaa tgctaaacgg tttgttaaaa aacgacaaaa aacgatgagg
    28501 gaggaaatta tgtaacagct caacccaacc aacgccacat catccgtcta gctgagaaac
    28561 aggtgtggcg tttgttttaa gtgggcaatc aaaagcgata acgtttttaa ttgtttattt
    28621 atcggagtaa caaatgacag acaaagccaa gctaatccag cttatccata ttgccaagtc
    28681 gcaactagca atggacgatt taagctaccg tgaaatgctg aaacggctca ccaataaaac
    28741 cagctcgacc aaatgcacgg tggtcgagtt gcacaaagtc ttacacgaac tgcaaaccaa
    28801 aggagcaaaa gtaaaatatt ttgccaaacg cagcaaaaaa ccgaccgctt acagcccggc
    28861 aacaggggaa acagcggtaa aaagccaaat tgctcataaa atccgagcgg tatggataac
    28921 aatgggcaaa cacggatttt taaacgaccc aagcgaaaaa gcattaaatg cttatatgcg
    28981 taaggttatc aataataaaa ctagaccaac aatattatta aatgtctctg cattagaggc
    29041 tggagatgca agttatatgc ttgaaatcct caaaaaatgg cataaacgtg tgatgattaa
    29101 agccctaagc aaaagttgca cagttgatcc aaagataagc tacgatgaat tagtggagtg
    29161 gtacaatgaa gttatgtaga tgcccgattt gccacagtga tatacaccta gaagctctcg
    29221 tagaagatga agccgggcgt gaactactcg gcaaaatcag ccaattaaca cacggtgttg
    29281 ctcaaccgat ggttgcttat ttaggcttat ttaaaccgca aaaaagtaac cttaataact
    29341 caagagcctt gaaaatactc aatgatgtat tagcactcta cccttgctca ttgctactgg
    29401 ctcaagccct atctgaaacg gtagtaagca tacgcaaaaa acgtcagcaa gcaatggaaa
    29461 acgggcagaa aattgaaccg ctaccgaacc ataactattt aaaatcggtg tatgaaacgc
    29521 aaaaagtcca ttttgctgtg gtgagaaccg gcaaaaccga acgcgaaaca atgaaagccg
    29581 aactggaaaa aacacgcaat gcgattgcct atgtgcaacg gtttgttgat ttaggacgtg
    29641 aagaggttgt taaaaatagc cctgagtatc agatttggtt agctcataaa cccaaataac
    29701 acaagccacc aaaaggtggc tttttaactt tttgaaagtg cgataaaacc tgatttaaac
    29761 aggctttaaa cgggttttaa gattgagttt aaaaaaatat ttcacaaaat aagaatggtt
    29821 ttttatacaa taaccgaaac ggtttttgga taggaaatag acaatggaat tagtggatat
    29881 ttttgaagaa aaagccccgg aagtgttaac cgatttagcc aaacatattg aagtcgcatt
    29941 agaagaaaaa tgccaattta gccccgaaaa ggcaaagcaa atcggtattg atattgccca
    30001 acaaatggct caaaactggg gtggcgaggt aatttatatt cctcgcaatt tgttgatggc
    30061 tcttaatgag cgagatatga aaatttacga agagtttaac ggtaacaatc accgtgaatt
    30121 ggctcgtaag tacaatgtat caatgcagtg ggtatataaa attgtgaaga aagtccataa
    30181 ggacgaagtt gcccgcaggc agatgagtat gtttgagatg taaatcattt tttcccagcc
    30241 ctgatcattt tcagggcttt ttgtgtatta aaagaacgca gtctttaaac ccgtttaaaa
    30301 tcaatttttc agcaaattct ttaaactcct tttaaagcaa ttttaatagg agtgtgctat
    30361 gtctcaaaat cttacctttc gccaaatttt tgaccgttta atcggacacg agggcggcta
    30421 tgtcaatcac ccacaagacc cgggcggaga aacccactgg ggaattacca aacgtaccgc
    30481 ggttgctaat ggttatatgt atgcaatgaa gaatatgacc cgtgagcaag cctaccaaat
    30541 ctacgaaaaa gccttctggc aacgttatcg ttgtgcagag cttaagccag cagttgctta
    30601 tcaatttttc gacgcagtgg ttaatcacgg ttttggtaat gctagtcgta tgttacagcg
    30661 tgcagtaggc gtagcggatg atggcattat tggcaatatt accctgcagg caattaatgc
    30721 aaaatctgaa agcgatctct taatgctatt taatgccgaa cgtctgaagt tttacacaaa
    30781 attacgcaac ttccccactt ttggtaaggg ttgggtcaat cgtgtggcag gcaatttaga
    30841 atacgcagca caagataatg aggtttaaaa atggcaaaac tatttaaaga actactctca
    30901 accaatggca cattatccac cactagcacc gtgcagtttt ttggcacatt gtgtgtattt
    30961 ggtttaatgg tttatgcagt aattacccaa caaccttatg ccgaaagcct attaaataat
    31021 gtgttgattt atctttttgg ggcaactacc gctaagggtg tagtaaccag ctatcaagcc
    31081 aaaattaaag ggggtatcca taaacaacaa agtacattta aagaggagga gggatgttaa
    31141 tgcaacaaat tttaggcgtg attggcgtca tcgcagcaat attgtcttat gctttgttta
    31201 aaagctggca actaaaacgt gagcgtaaaa agaatgccat attacaagct gaaaaacagc
    31261 aacaagcggt tgaaattgag caaaaaaaag cagaggtaaa atatgtgtat atatcaaaga
    31321 aaaacaatga aaaagttact cgcagctctg ctagcgatat tgatgacaag ttgcagcaac
    31381 acggttactt ccgtgacaac aacaggttgc acggcgttca gtctgattta tccgaaccgt
    31441 gcggatacgc tggaaacgaa acgtcagatt cttgctcata atctgacttt tgaggaaatt
    31501 tgccaaaatg aaatacatta aaaaattacg taaacgctgg caaatttggc gttttttaaa
    31561 aaatcatcct gaggttgcac aacgccgaga tttattagcg attgccatta aacagggcgt
    31621 agaaaaccct gtagggcgtg caagacggag ggagccataa tggatttatt tacagtcttt
    31681 tgggagtcta tccgtgctaa ctttggtttt atttctgcgt tactcaccat tgttgttacg
    31741 gcattttggc tcaagctaga tagcaaatac gccaagaaaa atgatcttgg tgaattgctg
    31801 gaaattgcca aaagccacga taaccgctta accaccttag aaaccaaggt agaaaacctg
    31861 ccaagtgcgg tggacgtgga aaagctgaaa accttagtca ctgatgtaaa aggcgatacc
    31921 aaagccacct caaagcaggt ggatagtatc agccaccaac tcggcttgtt attagaagca
    31981 aaattaaagg aatgatatgt cgttaagaga tttactcact caagaccaac ggctggtgat
    32041 tttacgctca ttagcggaag ccggctatga tgccaatgag tcaattatta gtgattgcct
    32101 agatttatac ggtcacgata ttagccgtga tttagttcgc acccatttaa gttggcttga
    32161 agagcaaggc ttacttacct tagaacgttt gcgagatggc tatatggtgg caagcattac
    32221 ccagcgtggc ttagatgttg ctcaagggcg tgttgtggta agtggcgtta aacgccctcg
    32281 ccctaaaatt taaacggtat ttaaggagcg tttaaatgag cgagaaaaat actcgtggtc
    32341 gagcatcaaa agtcgattta ctgccgccga atattaaaac ccaactggcg atgatgttgc
    32401 gtgataagca gtactctcag tcacaaattt tagaagaaat taatgactta attcgggatt
    32461 gtggcttgcc tgatgaaatg ctgttaagca aaaccggatt aaaccgctac gcaagccgta
    32521 tggaacgctt aggggcaaaa atccgtaatg cccgggaagt tgccgaaatt tggactaagc
    32581 aattcggcga aatgccacaa accgatattg gcaaaaccct aatggaaatg gtcaagcaaa
    32641 ttgcctttga aacctcattg aaattaggcg aacaagaagg cggtattgag ccaaaacagc
    32701 ttgctttgct ctcgtctgct attcaacgtt tagaacaagc cgaaagtttg agttttaaac
    32761 gggaacaggc aatccgtaaa gaggtcgcac aacaagccgc tgaaaccgct gaaaaagtgg
    32821 tggtgcaagc gggtttatcc gctgaaaccg tccgtacgat taaagagcaa attttaggaa
    32881 ttgcataatg gcactactca ataacagacc gctgaatgaa ttagcccctg aatgccgttc
    32941 gtttttagag agcattcacg cttttaatcc taacgaactg ttattgggct atcaaaagcg
    33001 gtggattgcg gacgacagcc aactcaaaat tgccgaaaaa acccgccgtt gcggtttaac
    33061 gtgggcagaa gcagcagata acgccttaat cgcaagcacc cgcaaatcag acggtggctc
    33121 tgatgtattt tacattggct caaataaaga gatggcacgt gaatatatcg atgctgtcgc
    33181 aatgtgggca aaagccttta atcgtgtggc tggagaaatc caagaagaga tctttgaaga
    33241 cgaagataaa gacatcttaa cctatgtaat ctacttctca tcgggtttta aggtcaaggc
    33301 attatccagt aatccgaaaa acctgcgtgg tatgcagggc gtggtggtga ttgatgaggc
    33361 ggcattccac gaatacttag cggaagtctt gaaggcggcg ttagctctca caatgtgggg
    33421 ggcgaaagtc cgtattattt cgacccacaa cggctcggaa aatctgttta atgaactgat
    33481 taccgatagc cgtgccggcc gcaaacgcta ctctgtgcat acgattacga ttgaagatgc
    33541 ctgccgtgat gggctatatc aacgtatctg ccaagtagtg acgaaaaaaa cgtggtcacc
    33601 ggaagcagaa aaagagtgga ttgaaaacct actcaacgac accgccagcg aagaagacgc
    33661 attggaagaa tacttctgcg taccgaagtt cggctcaggc ttatggctct ctcgtgcttt
    33721 gattgaacgg cagatgagcg aagccacgcc tgttatccgc tttacggcaa aagatggttt
    33781 cagccttgtg gctgaaccga cacgctatgc ggagattgcc gaatggtgtg aattagaact
    33841 tgagccgata ttggcaaccc ttaaccctga tttgttgcat tttatgggcg aagactttgc
    33901 acgcagcggc gatatgacct ctctggtggt gcttgctcag caacaaaacc ttgttcgtaa
    33961 agtcgccttt attgttgagc ttggcaatat gccctacaag caacaagagc aaatcgtgat
    34021 gtttatgctt aaacgcttac cgcattttgc cggtggggct tttgacgccc gaggcaatgg
    34081 tggctattta gccgaagctg cacgagatga atttagcacc ttgattgact gcgtgcagtt
    34141 atcggaaaaa tggtatcggg aacacaccgc cccctttaaa gcggcattgg aagacggcga
    34201 gctggaagct attccaaaag atgcggatat tttggctgat ttgcgttcat ttcaggtggt
    34261 gaaaggcgta ccacgcatac cggataaacg cactaaaagt gcagatggga aaagcaaacg
    34321 acacggtgat accgcaattt ctttgctgtt ggctcattat gccagccgac aattagtgca
    34381 aatgccagtt gagccggtat cacgccgtcc acgccatagc caacaattaa cagaaggata
    34441 ttaacaaatg agatatgtac cactttgttt aatgatgtgt gcctcattct acctatttat
    34501 taacaacatt gacggctggg gttggggaat tcttttatct tttattttag cgagtgcagc
    34561 ttatggcaaa gaagaataat ttaattagtg aaattgccac ccgtgccaga agtattgatt
    34621 actgggcatt tggctattat ctgccgaacc ctgacccgat tttaaaacga atggggaaag
    34681 atatttccgt ttaccgtgag ttgttatcag acgggcaagt tcgctcaggc gtacgccgtc
    34741 gaaaagcggc gattaaaggc ttagagtggc gaattaccac cactaacaat gagaaagtgg
    34801 acgagcagat ttatcaaata tttaaccgct tgccgatgaa taacctgatt accgaaatgc
    34861 tcaatgcagc actctatggc taccatatca gcgaggttat ttgggcaaaa gagggtgagt
    34921 tatttgtgcc aacagaaatc attggtaaaa agcctgaatg gtttatgttt gacgacgaaa
    34981 acctactgcg tttccgtacc aaagaaaact gggcggacgg cgaattatta ccagatgata
    35041 agttcttgct tactacgcaa gaagccaccc aagataatcc ctacggttta ggcgatttat
    35101 ccctctgctt ttgggcggcg acctttaaaa aaggcggttt caaatactgg ttggaattta
    35161 ccgaaaaata cggctcaccg tggttgattg gcaaacaccc tcgccaagct cacaatacag
    35221 ataaagagcg actagctgat gccctagagg gaatgattag ctcagcgatt gcggttatcc
    35281 ctgaagatag ttcggtcgaa atcaaagatg cagcgggcaa atctgccagc accgatgcct
    35341 ttgaaaagtt cctcaatttt tgcaaggcag agatcaatat cgccctactt gggcaaaacc
    35401 aaaccaccga gcaagaaagc aaccgtgcca gtgcacaagt aggcttggaa gttatggaag
    35461 atattcgctc ggacgaccaa gcgatgattg aagccacctt caacaccttg ttgcagtgga
    35521 ttgtgaaata taacttcaat gtggaacaac tgccgaagtt tgaattttac gagcaagaag
    35581 ccatcaacac cgaccaagtt gaacgagaca gtaaactcca cgcaatgggc gtgcgttttt
    35641 ccaaagccta ctttgagcgt gaatatggct ttgaggcggg tgatattgag gtgcaagcgg
    35701 tcgaaaaagc ggaaaaatct gcaaatacca cagaatttag cgaaggacaa cgccgaaccc
    35761 aagtgccaat gcctgtgcca attttggaac agctagaaca cgagggagaa gcaatggtgg
    35821 acgattggct acaaggggtg agagataaac tcgctcaagc agatagtctg gaggatttcc
    35881 aaagtcagct agatagcctt atccctgaat taagctttgc cgaatatggc gagctgttgg
    35941 cgttaggctc aacggtggca gagcttgcag gcagacaatc ggtaaacgat gaaattaagg
    36001 caaaacgcaa tgcgtaaatt tacctttaaa cagcaagccc aatattttga gaaaaaactc
    36061 aatctccgca cgaatagcta ccttgatatt ctgggcgaag agcacgacta ttttttcgtg
    36121 gtggctgggg cgaatcgtaa tgaagtgttg agtgagtttc gcaaggcggt ggatgaggct
    36181 attcacaacg gcgaaacctt agagaacttt cgcaagcggt ttgatgaggt tgtggcaaaa
    36241 acaggctggg agtacaacgg cggacgaaat tggcgaacac ggattattta cgataccaat
    36301 gtttacgggg cgtataaccg tggacgtttg caacaacatt tagagcttgc ggaggatatg
    36361 ccttattggg aatatcaaca caacgacaac gcccaccctc gcccacagca cgtggcgtgg
    36421 gacggcttgg tgctgcgtta tgacgatccg ttttggaaat atcactatcc aataaaagcc
    36481 tacggttgcc actgcaccgt gcaagcgtta gatgattttg acctcaagca cgatggtaaa
    36541 accgtcagcc caccacctga aattgagttt gaacaaaaaa cggtaggcgt gcggacaggc
    36601 aattcacgag tcgtaactgt gccgaaaggg gttgatgtgg gttttacacc gcataatttt
    36661 gataatctca aagccgggag agcagcaaat gtagataagt tgctaatgca aaaaatggtt
    36721 accgccgatc ctatatttgc ctccaaagca gtcagtgatt tattggcaaa agagccggtt
    36781 gcaaagttac ataaccaagc ctttaaaaat tgggtaaatg atgttgccaa tgctaaagga
    36841 agtgcattat cccaagtaga gaatatgcag ttgttagggg tactacctgt tgcggtgatt
    36901 gaacggttac agcagatcaa tttagctccg caatctgcaa ttattgcaat aacaaaaggc
    36961 gatatggtac acgctctacg agagctaaaa caaaataccg gtattgccgt accgttggaa
    37021 ttttgggaaa agttgcctgc aaagttacaa tacccaaatg ccatcttgtt ggaaaaacaa
    37081 caagcagaaa ctacattgct ctttatctat gacgtagctc aaaccaaaat tgccgtaaaa
    37141 gtcaattatg atgcaacttt acaagataga cacggagcaa aaggacggca gaaagtctta
    37201 ctgaatcgag cggttacagg tagtttattc caagatacaa ctggattgaa aagttcaaag
    37261 tatgaagtgc tattgggaga aatgtagcgg cgaaggactc gaaccttata atgtaggtta
    37321 aacctacaac ctttaccaat taggaaacta ccgctacatt gtgtaaattc actatacccc
    37381 taaattattt tttaatcaat aggagaaaca aaatggcaga aaaacaactt tcctttaaag
    37441 tagccgaaat agtgccaatg tcagctatta acactatgcg gcacttaatt agtttggcat
    37501 tacaagcgaa ttatgagaga gaaaaatctc gtgcttgata atttcaatgg cttgtagttc
    37561 gggaactata ttatctgaaa caagccattg ttttgttgat gtcagacatt tagcgatgaa
    37621 atccataatt aaaccattag cagtagctcg ttgttccgga gtaagttgta accacgacgc
    37681 ctctgtttgt tggtctaatg tataaagaaa cataacggct tctttttctg ataatgggtc
    37741 aagtggtaat ttttcagctt ttgtgactaa ccattgacgg gaaagcaata aatcataaac
    37801 cagttctatt gcttgttctg catttaccat catattctaa ttccttaata tatcagggca
    37861 acattaccca atctatattt tactgagatt tgcttaatga tcaaaatcac cctcaacaac
    37921 acccaagcgg tcaaatcgtt acaacaaatt gcaaatcagc tggaacaacc tcgccgactg
    37981 tatggcattt taggggaaac cctaaagaaa attcacactg cacgctttaa agcggaacaa
    38041 gaccccgatg gcaacaattg gaaaccgtta gccagtagca cgcttgcttt aaaacgtaaa
    38101 cgaggtaagt ccactaaaat cttacggcaa gacggctacc ttgccgataa aacggcttac
    38161 aatgttacaa gtgataatgt tgagtttggc tcgcaggaag tgtatgcacg gttgcatcaa
    38221 ttcggcggta aagccggacg aggcaaaaaa gtgactgtac cggctcgccc gtggttaggc
    38281 gtaggcgata aagatgagca gttactttta cgcaaagctg agtggcattt agggcaaatt
    38341 atcggtcgta tgggaaaata accgcttaaa atcaaattta acgcataaaa acgcctctgt
    38401 gcgtttttat tttttattcg cctgattatt cgactcaaaa aatttaaacg ctgttaaacg
    38461 catttaaacg gtatttaaac ggtattgccg attgtgataa atcctcttta ttaaaacacc
    38521 ctcgaaaaat ctttaaacca gtttaaaagc taatttctct ccaaatcctt attattgccc
    38581 catcacggag gcaatatgac ccaaattgaa atttttaaag ccggtaagcg ttatgacgcc
    38641 aacggcacgt taattgacat tacccctgaa atgttgcaac agaccgttgc tgcctataac
    38701 cccgaatttc acgaagctcc gctggtgatc ggacacccga aatccaacaa ccctgcgtgg
    38761 gggtgggtaa aagccctttc ccttgagggc gatgtactaa aagcagaagt agatcagcta
    38821 gacgctgagt tttcagaaat ggtggcaagc ggcaaattca aaaaagtctc ggctgctttt
    38881 tacttacctg atagcccgaa taacccacat aaaggcgtgt tatcgctacg ccacgtgggg
    38941 tttttagggg caatgccacc ggcggttaaa ggcttaaagc aggtggaatt tgcagaagat
    39001 gatgattttg tcgtcttttc tgaatggggg gttgcaagtc tatttagtcg ttttcgtgat
    39061 tggttggtga gtgagttcgg gcttgaaaaa gccgataaag ctcttccccc acacgaaatt
    39121 gattggctga aagaagaggc aatgcgaagc caaattagca aacaggtaca cgccgaacag
    39181 gcaacacctg aaccgatttt taatgaaccc gcacaaccag aaggagaacc tgaaatgagt
    39241 gcagaagaga aggaagagct tgaacgctta aaagcggaaa acgagcagtt gaaagccgaa
    39301 aaagcccaag ccgaagcaga gaaaatggaa gcagagctgg aagctgaaaa agctggtaat
    39361 gcagagtttt gtgaaggttt agtcaagcaa ggcaaacttg ccccggtggc aaaagcggcg
    39421 tttgtgcagg ctttaaataa cctttcagta ttaaaagccg gtcgtgagcc ggaatttaat
    39481 gagggcgaag atgtggtttc acagtttaag tctgcaatgt cgcaatcacc acagattatc
    39541 caatttggag aatatgccac agcagataaa gccaacccac aagaagcgga agcagtggaa
    39601 tatgctgaag gcacagatcc agtttcgatt gaagccgacc aaaaagtgcg agcttatatg
    39661 aaagcccaca atgtaggcta cgctgcggca tttaatgcga tttatcgcta atgtttaacc
    39721 cacaactgaa aaggaatact aatgacaact cataacttac aaaaactgcg tgtacaagac
    39781 cccgttttaa ccgagctggc acaaggctat cacaataacg agctggttgc cgagacctta
    39841 atgccgactg tcgaaatcga aaaggaagca ggcaaaatcc caactttcgg gcgtttagct
    39901 ttccgattgc cttctaccgt gcgtaacttg cgtggttcat ctaaccgttt agatccagaa
    39961 gatattgggg cgattgatgt ggcgttagaa gaacacgatg tggaatatgc cattgattat
    40021 cgtgaagaga acgaagccat tttttcttta cgtcaatttg ccttaaatac cacccaagat
    40081 gtgattgcgt taggtcgtga aaaagcggtt gcaaccctcg ccttagatga aagccgttat
    40141 gaaacagaca ataaagtcac gctgtccggc accagtcaat tcagccataa agatgctgat
    40201 gtgtttaagg tatttgatac cgctattcgt gccgtgaagc gggctatcgg tcgtaaaccg
    40261 aatgtgtgtg tgatttccgg tgatgtgtgg gcagcgttaa aagaacatcc ggcagtggtt
    40321 gaaaagctca aatacagtca agtagcgatt gttacccctg aggtgtttgc caaactgatc
    40381 ggcattgaga ccgtaaaaat cggtgaagcg gtgtatgaag aaagcggtca gctcaaagat
    40441 atttggagca aagatattgt ggtggcgtat gttgctcctc gttcgaccgg aggcaaaggc
    40501 acggtttacg agccgtctta tggttacacg gttcgccgtc aaaaaggctt atttgttgat
    40561 acttataaag agagtggcgg caaaattgaa gtggtacgca cgaccgatat tcacaaacct
    40621 catttagtgg gtgcggcagc aggctattta attaaagctg ccgtataagc cacataccat
    40681 agggtgcgtt tactaacgca ccattatctc aaacacatta catagaggaa aaaacgatgg
    40741 ctaaatttat tgttttacat accccaattt tacacgatgg caaacgctat gaggtggggg
    40801 cagaaattga actcaccgct gaacaagctg agaacaatgc gatcaactta aaagcggtag
    40861 aagctgttcc aaaaccacct aaaaatgcct caaaacctga agatgagcca aaacccgaag
    40921 gtgaacctaa gcctgaagat gcagtgacgg aagatgatgt gaagctggct gaagcaaaaa
    40981 ttgcagaagc agagcaacca aaagggaata aaaaataatg tatgtgcaga ttgatgagct
    41041 actcacggcg tttagtcgca agattgtggt gcagctcagt aatgatgaac cgacagcgac
    41101 cgagcctgat ttaaagatgc ttgaaacggc agttaaagtg gcaaatgagc gaattgatgc
    41161 agcgttacgc tctcgctata ccttgccgct ggctgatgtg cctacgctta ttaatcagca
    41221 cgcattaacc cttgcccgtt attggcttta tgcccgccgt cctgaaacca aaatgccgga
    41281 caccgtgaag gaaacctacc aacaagcggt gaaagagttg gaacagattg caacaggcaa
    41341 actgcattta ggcattgctg aaagcagtca aattttagaa gacgatgttt tagccgataa
    41401 cagtgaatat gaagttcggg cgaacgctcg cattgatacc agtcgttatt aaagattatg
    41461 tcagccacac aaccgatttt agaagatatt tgccaacact tgcggcaagc attgcccgag
    41521 tgggaggtgg aactcttccc gaatgaccca agcacctatc atctctcgca cattaacggt
    41581 gcggtgttga ttagctattt ggcaagcaaa tttgagaaac ctcgcactac acaagcggtg
    41641 ctgcaggttc gccacgttca gattgcttta acggtgctga cccgtgattt gcataatgat
    41701 gtcggggcgt tggatttgct ggataaattg cggttattgg ttgtggggtt taagcctcaa
    41761 aactgcggtg agtgttggtt gattgatgaa ttttttaatg gctcaagcga agaaaccggg
    41821 ctttggcaat atcagttgat tttgcaaacc gaaactcagc aggtgcaaca ccaagcggtc
    41881 gaaaatctgc caaaatttgt aaccgctcat cttcgccgtc aagaccagcc tggttcgccc
    41941 ggacttaaaa cttaaatcgt agggtgcgtt tgctaacgca ccatcaacat cacctgattt
    42001 tacggtgcgt tggcaaacgc accctacaag gagaatatta tgacgtttca ccacggttct
    42061 gaaaccgaac gagtaaacgg tggctctgtg ccggtatcgg tggttgatag tgccattatc
    42121 ggcattgtcg gtactgcccc aatcggcgca gttaatgaat tgaccgtctg tttaaccaaa
    42181 aaagattttg ccaaattcgg cacgatttta aaccaaggct tcaccttacc tgatgccttt
    42241 gatattttag ctcgttacgc agcgggtaaa gtgtatgtgg tgaatgtgct tgacccttcc
    42301 cgccaccgta ctaccgtgac cgatgaagtc ctcacactgg atcgcaatac gctgaccgct
    42361 caaacagcga aaaccggcat tattgcggtg acgagagtga aagacggtgc aaacgtgctg
    42421 aatgctgatc ggtattcggt cgatctgcaa accggcgtgg tgacctttgt ctctgcaccg
    42481 tctgccccga aaattagcta tatctatgct gacccgagca aagtgaccga agaggacatt
    42541 aaaggcgggg tggatagctt aaccggtaaa cgcaaaggtt ttgaactatt gcgtgatggt
    42601 tttaaccttt acggtgcgga tgccaaaatc ttaatctgtc cggaatttga taaaaccgca
    42661 agctgtgccg ctgcccttgc cacccttgcc gaccaactgc acgctaaggc gtatattcaa
    42721 ctgccgaaag ggacgagctt atcaaaagct attcaaggac gtggcccggc agggacgatt
    42781 aacgcctcgg caagcaatga gaacgtacgc catttcttcc cgtatgcgtt ggggtcaagc
    42841 aatactcttg aaagccttgc gacccacgcc gccggtttgc gtatgttgat tgatgtggag
    42901 aagggctact ggttcagcac ttccaataaa gagctgaaag gggtgatcgg gttggaaatt
    42961 ccgctgacgg ctcgggtgga cgataagcaa agcgaaacta atctgctcaa tgcggtaggg
    43021 attaccacca ttttcaatag ttttggcaca ggcttccgct tatggggcaa ccgttcgtct
    43081 aacttcccga ctgtgaccca tatcagcaac tttgaagtcg cctctcgcac aggcgatatt
    43141 attgatgaaa gcattcgcca agccgaattg cagtttattg atttaccgat tgatgatgcg
    43201 ttgctggata gcttccttga aaccatcgac actttcctgc gtacgcagaa atcgttggta
    43261 ggttattcgg tgagcttaga ctatgaatat gacttggttg atgcattcag ccgtgggcaa
    43321 gtcccgattg tgtatgaata cactcctaaa attccgggtg aacgtatcag caataaatct
    43381 gtgatgaccc gtaaatactt ggcaaacttg gtgtcgcaac gataagggga aataaatgag
    43441 tattgcaatt aaccaaattg taaacggaaa tgtttacatt aacggcaaca gccaaatggg
    43501 gcgagtgaat gaggtcaaaa tccccgatat tgagtttgaa tttatcgaac acaaaggcct
    43561 tggaatgcac ggtgtgatta aattacctgc cggcacgaat gcgattgaag cagaaatcac
    43621 ttgggacagt ttttatccgg aagtgcgtgc ggtaatgctt aatccgtata aatacactca
    43681 gttgcaaatc cgctctaacc tgcaagtgtt taactcgcag ggcttggcag ccgaagagcc
    43741 gatggtcacc attatgaatg tgtctgccgg taaaatcggc ggcacagggc ataaaaacaa
    43801 agaaaatgcc gaatttgccg acacgttaaa tgtacattca attaaacaga ccgttgccgg
    43861 tcgtgagctg ttatttattg atgtgctggc gaatatctac cgtgtgaacg gggtggatgt
    43921 gttgcaaaaa ttccgcacca atatcggtta gtaaaatctt taaagccgtt taaaatcaat
    43981 ttaaacaccg tttaagtaaa ctcctttgtg aagttaaaca acaaccactc acaaaggagt
    44041 ttttttatgg ctaacgcagc tcaacaagta ttaaccaatc ttcgtgtaca aaccacttat
    44101 aaattgcaat accctatcgc tttaccagat ggtagagtta ttgaaacctt agatgtacgt
    44161 cgtccaaaag gtaaagattt tcgtttgctt gatgaaaaag ggtttgaccc tgaaaaagat
    44221 ggcgtaaaaa ttctacgctt ctatattcaa cagctcacca cacttgtgcc ggaagatatt
    44281 gatgaactcg atgctgccga tattaagggc ttaaataacc tattggaaga tatgcttatt
    44341 gagggaaagt ccgacaaata agtgaactag ataccgttta tgctgatctc gcttggtggt
    44401 ttaagttttc accaagcgag cttttagaat tggatttagt ggatattccg aaatgggtag
    44461 aacagatgaa caggcaaata aaagccggtt atgggcagat tttacggtag agccaaataa
    44521 agggttcggt ggctaaagta agtaaaatgg cgacgcccca agctagatac acaccaaccc
    44581 acgccaagat tgtcaaaatc aatccataaa tgagtacaaa actaacgccc tcgcttaggc
    44641 tttgtataga ttgaatcgca tctaaccagt agaagatact ccaaccgact aatgctaccg
    44701 ctaacaacca tttagcggtg cattctgttc ttgttacaca agcctcgaaa ccagcccact
    44761 cagtaattcg attatttaat ttagtcagca tacaccacct ccttaaactt tccctatttt
    44821 acgctaagga tttaaccaat ggcaaataat ttagctatcg gaatggtaat tggtgcaagc
    44881 ctatcatcag gttttcacgg tgctttttca ggagcaaaga aaaagctaga acaattagaa
    44941 caggctgtcg ctaaaaatca gcaaagccat tctaaattag gttctgagct tactgcattg
    45001 cgtagtaaac aagctactct ctatgcagaa atgagccgag ccagtagtaa aggtggtgct
    45061 gggcttgctc ttatgcaaca ggaatataaa aaaattggca aagaaattgc caatattagc
    45121 cgagagcaac gcaaatggag tacagaatta gaaaaatcta ttgctaagca aactaaatta
    45181 cgcaatgcaa tggaacgtca tacacaagct aaagaaaacc gagacgagct taaagggaaa
    45241 ataattggaa cggtagcttc tgcggcaatg ggtgtcggcg tgatgaaaac ctatatggag
    45301 caagaagaag ctgcgaataa tcttaaaatt tcaatgctga aagcagatgg ttctttcggg
    45361 aaatttgaag agattggcaa gattgctgac acattaggga cagatttacc gggaacaaaa
    45421 aaagatttct acaagctcgc aatggcatta aagaaacaag gtgtttctga tgatgtgctg
    45481 accggagggg cattaaaaac ttctgccgaa ctgaatgtct tgcttgatat ggatcaggaa
    45541 agtggtggag agtttttagc gaagtttatg gaatctcacc ggctcaatga aaatgagtta
    45601 ccacaagcag ccgattatct gcaacgagca atgtttgccg gcggtttaag taaagagcag
    45661 atgtatgaat cgatgaaata ctatgctcca aaactgaact caatgaaact tacaggggct
    45721 gagaataccg aaaaagtgct ggcgattgaa gcaatggcag gtcagcaagg cttagagggt
    45781 tcaacctttg gtacgggctt aaatatgatg ttaagccgta tgaataaagg accggaaatg
    45841 ctcaaggcag cagccaaagg aatgaaagcc gaagctcgag agatgatgga ggctgtcgga
    45901 gtagaattta atttctggga taaaaacggc tcattcaaag gcattgatgg aatgctggct
    45961 gaaatgacga aatttgaaaa aatccgcaaa aaattcggtg acaaaggtgt aggtttagtt
    46021 gcagaagagc tgtttggcat tgaaggtggg cgtcttgctg atattttggc tatgaaaggt
    46081 caaaaaggct tagatgaaat gctgagtaaa atgcgtgaac aagccagcct tcaagatcgc
    46141 attaacctta aaacaaatac attaggtgct gccctagaat ctttaggagg agcgtgggaa
    46201 acctttgtgg gggatattgg ctctgtcttt gccgaagata taaaagggtt ttcaaaaggc
    46261 ttgcaatctg cggtggaatg gcttgtgcca tttgtatcaa aacataaaga ggcgattaaa
    46321 tttgtaggtg gtttggttgc tggacttgtt attttacgag gcgttttaca agctggttct
    46381 tttgttattc aaacggtaac tatggcaatg aacgccctca aaattgcagc aatgaccaat
    46441 cctattgtct tagtcattgg agcaatcgca gcgggtgctt acttgattta tagccattgg
    46501 gatagcattg ccccttggtt tgcaaatctt tggcaaaatg tgaccgctta cttcaatcaa
    46561 ttttggactt gggtaaaagg tgtttggtcg ggcgtgacgg actgggtgtc ttctgtgtgg
    46621 aatggtgtag ccactttttt cgggcagtta tggactacga ttactagctt atttagtgta
    46681 gcttggacaa atatccaaac aatatgggga ggagcaacag cttggttctc tggtttatgg
    46741 gatgggatta agtctgcggc ttttggtatg ttcgacagta ttagccaaaa agtcagctct
    46801 gttgtagcaa gcattaagag ctttttaggc tttggcgatg atgtggataa aatgggggca
    46861 aaaattggca atgtagctac ccaagtcatc agcaaagaac actcaggaca actcagcaaa
    46921 accgcagata tggcagccaa tacagggttc tctagcggtg gtttcaccgg gcaaggcggt
    46981 aaatataccc ctgccggtat tgtgcataag ggcgaatatg tgatgacgaa agaagcgacc
    47041 tctcgtttgg gtgttggaat gctggatagc ttaaactatg gcggtgctat gggtttcact
    47101 caacctgttt tgcaaccgat tttaggtaag caaaaaggct tttttggctc actttgggac
    47161 gatgtgaaat tcggggcaaa ctttgtcggc aatctgcttg gcttaaatca aggcagcctg
    47221 aaaacgcctg attttaaccc aaatgcgacc aatgcaagcg gtcaaaattc gtcgattttt
    47281 agcaattatg aaccactcaa ccgaaatgcg gtaacacagc aggaaagctc gcaagcaccg
    47341 cagatagtcg tgaactttaa cccgacaatc aatgtaaacg gtaatgctac gcaagaggct
    47401 caatcgcaaa ttatgcagca gttgcaaatg agtgcttatg agtttgagaa attgctcaat
    47461 cgtgtgctag accaacgaag ccgtagagcc tattaggagt gatttatgta ttttatgtta
    47521 ggcaatattg cctttgagcc ggtgaattta actgaattta gcgagcaaca taccgccgaa
    47581 tttgccgaac acgctgtgct aaaaggcaaa cctcgtttac aagcgatggg cgaaaagcta
    47641 aatgaactta atttttctct tcggttgcac cataccatag gcggtgtaga gagccgttat
    47701 caagccttgc ttgaagccaa agcgaagcaa gcggcattgg cgttaatttg gggcagtcgt
    47761 tataagggag attatgtaat tgtcgatatt tcatcgacca cattgttcac cgatggcaga
    47821 ggcaatgccc tcgctcgtga aatgcaaatc agcctgaaag agtttgtggg cgataatgac
    47881 ggtggtgtgt tgggtgaggc gttaaatttt ggtggtggct cactgcttgg ctctattttg
    47941 ccaagcggtg cggtaactac cctatctcaa atcaaaacag cagtcagccg aggtgtggag
    48001 ctttataaca gcggcaaacg cttagttgac gaagtgcaaa ataccgtggc gattattcgg
    48061 cagttgaaaa atgacccggc aactgcattg gcatatttac ccgctgcatt agcgaattta
    48121 gacggggcat tggggcattt tggtaatttg accggctcat cggcgacctt gggcggtttg
    48181 caggagaaac taccggcagc agtggtcttt agcgaggaaa tcggcgagat ttatcagagt
    48241 ttgcaaacaa tgcgaagcag tttgggtaat gcctcggcaa atagctggga taattggttt
    48301 acaccggcag agagtgcatt gacagaggtc acagacagct ttgaccatct agcaaaaccg
    48361 gtggcaaaaa tgaccgcttg gattgtgctg cgtaccgatg aggaggcaga aaatgacaca
    48421 gttgcttaaa catatcgtca aacagggtga gcgttgggat aacttgagct atcaatacta
    48481 cggcaatgcg tttgaatatg gtcgaattat tgatgccaat ccgcatatcg gtttttgcga
    48541 ggtgttgccg ataggggtaa caatttatat ccctgtactt aacgttaagc caacgaataa
    48601 tgaaaatatg ccaccgtggc taagggggcg tgatgcgtaa tgtaccgaag cccgattttt
    48661 cactgtttta tgaaaaaacc aatatcactg ccgagattga gccacatttg gtgcaactga
    48721 cctatatcga ccatttggaa ggtcaatcgg acgaactcac tgtggaattt gaagacattc
    48781 aaggcaaatg gatacgccaa tggttcccga ctcaggggga taagctcaaa gcggcgattg
    48841 gctatcaggg cgaatttctg acggacatcg gtgagtttga aattgatgaa gtggaatata
    48901 gttataagcc ttccactatt aacttaaaag ctctaagcac cggtatcagc aaagccaacc
    48961 gtactttaaa accgaaagcc tatgagaata cgactcttgc tcaggtggtg gcaaaggtag
    49021 cagataacct gaaattaaag gtagtcggta agattaaggc tatcccgatt aaacggatca
    49081 cccaatatca agagcgggac gtggagtttt tggcacgcct tgcccgagag tatcatcaca
    49141 gctttaaaat cgtgggcaat cagctggtct ttaccgataa aagcgaactg gggcaaagtg
    49201 agccggtgtt aatgttggaa gaacgggata ctatttccct acgtttgcga gatcggatta
    49261 aagacactgc aaaggcagtg gaaatcagcg gctatgatac aagcggtaaa aaagtggtga
    49321 aaaaacgcaa aaaggcaaaa gccttacgcc cgaatataaa acaagctaaa tcagccagtg
    49381 aagatacgct gaaaatcatc acacgaggcg aaagtcaaga acagattgat gcacggggtg
    49441 aggctgcgtt ggctacgcaa aatgaagatc aggcggctgg tgatattgag cttatcggca
    49501 atcctaaatt ggttgccgga gcaacgattt tattgcgtaa ctttggcgtg tttagtggca
    49561 agtatctgat taaatcatca cgccatacga ttagccgtag cggtggttat actactcata
    49621 ttgaggtcag aatgttggag tttattgccg atgatttaat cacattaggt atggaggctg
    49681 gcaatgcaaa cgcataattt tggggcgact tatcaagaag ggattgtatc cgctgttgat
    49741 ccgaaaacgc acaaagtgcg gtgtaaaatc cctgcgctgg aagatttaga aactgcttgg
    49801 ctgtcgtttt tgaccccgaa cgccggtggc aatcagtttt actgtttgcc cgatgtgggc
    49861 gagctggtgg caatgctact tgatgcacaa ggcgaaggtg gctgtgtgtt tggggcaatt
    49921 tataacgagc aagacccgac accggttgcc gatagcgata tgtgggttaa gaaattcagc
    49981 aatggcacgg agatttcgca taatcgcaaa accggcgatg tggtagtgaa gactaacggg
    50041 catttaaccg caacggctca aggtggggct acgattaatg caaacaccgt agtcaatggc
    50101 acattgcacg ctaccggtaa aatcacctct gacgaagaag tctctgctcc tgttgtgaaa
    50161 caaggcacgg tagagcttgg cacacataaa cacggcggta gccctgaacc taataagtaa
    50221 ttctttaaac cagtttaaaa tcccttttaa agatagccct gtatcatcag ggctatgaac
    50281 agacaaactt ccattcaatt aacccactgg caacttgcac cctcaaccga tacagttgag
    50341 gtggtgcaag gtattgatga tattcatctt tgtattgtca atatcctttc aacccaaaaa
    50401 ggcacggata ttttacgccc tgaatttggc tctgaccact ttcgctacat tgactaccct
    50461 gaagatgtgg ctatcccgaa ttttgtacgg gaaattacaa ccgccttgca aaaatgggaa
    50521 aaacgcattg aggttgaaga agtattggtg gacggttcag ctccgcattt tatttttact
    50581 gtgatgtggc acttaaccga tgatgtttat cgtgaaattt accgaacaac ggtataagga
    50641 taggcaatga cagaagcgat taaaatcatt gatgacgatg taaaaaccgt acttgccgaa
    50701 gcgattgccg attatgaaaa acgcaccggt aaaatcttac aacctgcaca tattgagcgt
    50761 tccattattc aaacctacgc ttaccgtgag cagttattac gcaaaggcat taatcacgcc
    50821 tttttacaaa cctttccgca gtttgcgacg gggattgcgt tggatttgtg cggggaaact
    50881 tttggttgtt atcggcttag agataaacct gcccgtactt tactacgctt ttcagtaagt
    50941 ggtgaacata cagctatagt tatccctaaa ggaacgcagg tggcaattaa tgacgagcta
    51001 tacttttcaa cgctcaatga cgatgttatt actccattaa ttacctatgt ggaaattgat
    51061 gcagagtgta atcaggttgg tgttatcggt aatggttggg aatcaggaag agtcagtaaa
    51121 ctaaaaacag cactggcaac taattataaa gtaacagttg aaaacattga tattacgagc
    51181 ggtgggttga tgcaggaaga agatgaacct taccgtaagc gaattttagc cgcaccggaa
    51241 gcctttagta actgtggctc tatcgctgca tatgattacc acgtccgctc tgtatcccaa
    51301 gatattgcag atgtgaatat ctctacgcct aggggtggct tggtaagaat tgcggtattg
    51361 acccaaaaag ggttaccgga tacccgatta cttgatgata ttaaaaaaca cgttagtgcc
    51421 gaacggctac gcccactttg cgatatggtc gaggttatta agcccacaga acgccgttat
    51481 caaattacgg cacgtttaat attgttagat ggctaccgtg aggatttggt aaaagcaaaa
    51541 gcccgagagg cattacaact ctatttgtca gacaagacta aaaaactcgg tttagacatt
    51601 gtgccgtcgg ccatcatttc agctttaaga gttgagggtg tttacgatgt gaatttaatt
    51661 cagccagtca aaactattat caacgaaaca gaatgggcga actgcacagc aatcagcgta
    51721 gaagtcgaag aggagcgtag agatggctag cttgcactat gcggacatta ttgtgcgaga
    51781 ccctaaatat aaagcattag cggatttaag caagcggtta aatttgctcg atacttcgca
    51841 aattatgact acgctggtgg atttattggg cgacgaatgt atcccattgc tggcagaaaa
    51901 atggtcggta accggctatg acggcttatt ggttgcagat agcgaaaact caaagcgagc
    51961 cttaattaaa agagcaatcg aactacatca actaaaaggc acgccttggt caattcgtga
    52021 agtattacgc agactaggtt tcggagaggt tgagattgac gagggactga aaaatcggct
    52081 gtatgaaagc acttatgtga gcggaatccc cgaaaacgaa cgctgggcgc actatgcgat
    52141 taggttgagt aacccgatta ccaatgagca agctcgcaat atccgtaagg tcttacgcaa
    52201 ttttgctccg gctcgttgct ctcttgcagt tttggattat aaagcagatc caattcgata
    52261 taacaataaa gcaacctaca acggcacata taaccacggt tcaagttaag gataaattat
    52321 ggcaggtgta caagaaaccg cacagtggga aaaccacgtt tacagaattg aagaaaatga
    52381 cccagtccac ggaggagaaa acggtgtaac caataagcca attaagcacc tagcaaaccg
    52441 cacgctctat ctgcgtagat tgctcaccga ggcaggacaa cgcattaacc cgaaaaaaat
    52501 cacagctacc agcaaaaata gtaatgattt aactgggcat actcacgaga ttgaccacgc
    52561 aagcctaacg caaaaaggca ttgtgcagtt aaatgcgagt atccaatcaa ctagcacaac
    52621 tgagtctgca accccttcag cagttaaagc agcctatgat aaagcagcaa cagcagaaca
    52681 aaaagcgaca ttggcaaatg agaaagtcga ggcgattaaa gtaggcggac gaaacctcct
    52741 cattcaatcc aacactccac acaacgggaa tgcgtactca gtcagatacg cattggctga
    52801 ggctccgaaa gtaggcgaag agattgtagt cactctatgg ggtgaactcg gctctcaacg
    52861 tactggaatt ggcgtttaca actctcaagg tgctacggag ttggcaaaac tggtcaaaat
    52921 tcgtgatgga gtttatcaag gtaaaggcat ttggcgtaag ccgatgagtg agggacgaga
    52981 gcgaacgcct aacgacacgc acctgaatgt gtatttttac ccgaaaaccg caacagataa
    53041 taaccgtatt gagcgaatcc agcttgagcg aggctcaatc ggtacagatt ggacgccaaa
    53101 cgaggcagac ttaaaggcag agattagtgc aatccgcact aaactaatcc ctgtacgggg
    53161 tggcaatgtt gctacaacct taaatgcggc taagcggtcg gattggttta gagaaatgca
    53221 aatcccttat caaagtggtt gggggtatta cacccaagca tttcataact cagggacgca
    53281 acacaactac agcaatttac cgctgacagg tcgttattat tttatcgccg aactctatca
    53341 taactctggt tattctcact tgcgtatcac ctatccaagt ttaaaacgca cctttgagag
    53401 tgctatcaac cttaacaatg aggatttgat tctagattgg cgtgaggtgg tgtttttaaa
    53461 agatggattg tatcaaggca cttttaaaac aacaggcaca ctagagtctg cttatcgtct
    53521 aagagcggct cgtgatgatg aacgttatca gccatacctg caaatggtcg atatgggcta
    53581 tgaccttgcc aatcccccgg taggaaaaaa caaaattatt ggcagtgttg attattacgt
    53641 taaaaacggc acaagcgagt cttccaaagc gacgatgatg gcagcggcaa tgcctgacaa
    53701 gaacgtattt tgggaagtag cattgtggaa tgcagcacag caacggaatg tattgtttaa
    53761 ggggtatagc aagactaaca atttgagtat tggtaaaacc accgacaatg agcgagaccg
    53821 agtacaagtc aatggcacaa tccaagccac cgcaccggct gataacgcca ataacgacca
    53881 agtgccgact acggcttggg tgcgtaagat ggcaaagatt ttagctgatg gcaaggtgag
    53941 taaagccggt gacacaatga ctgggcattt attcatcaag aatggggaat actctgcggt
    54001 acatacctat aacacaagtg gttggttcgc aaaatgggag gcggccccat tatctgattc
    54061 gcactttgct gccattgtat atgccaacga cagagagaca gttatcaatc gggtgttaat
    54121 tcccaagaag aatggcacag tcgctttaat taatgatatt gccgataccg ttagagccgc
    54181 tgtgagtcgt agtgataaat cccgtagtag aacttttcct ctgcaaacgg ggaaaaaata
    54241 tacaaccaca gggagcgtta cgatttatcc agatggccga attgtgcaga ttttccactt
    54301 aaaaaatatt aaagcggcgt ggtttgatta tgaggcaagt gcggtaggag ataggcatag
    54361 agttgtcaat atccctcttt ggactgcgat gccaaataaa atttttgatg tcgatgttaa
    54421 aactgttcgc ccctcaaata atgcctcaac ttactacata gaagcggctg aatggctgag
    54481 tgcgtggcag atttacggta atgacacgaa caaatcaagt gtcaatatta atctatctcg
    54541 attcagaggt ggaaaggatg aagatatgga cttgtatgta acagttgagg gatattaaat
    54601 ggcatatttt gtgaatgtga ttgatgagca aggcaattat gagctaattg atgatgagtt
    54661 tattgggctg tatccggatt tggattttaa cacattaccc aaactcacgg ataagcaata
    54721 tcagacctac cttgccaaag caaacggcaa agagcgattt gtaaatggcg aatttgtgta
    54781 tgagcaaatt aaggtggata tgcaagcggt catttccgct gaaaaatctg caaagttagc
    54841 agagattaac caaaaagcac aagcctttat caatgattta gccaaataca acgaaacgcc
    54901 taagtttgag cgtgatactt ggcttgagca agcgaaagag gcaaaagcgt gggtggctga
    54961 cccaaccgta caaacgccaa cccttgagtt aattgcgcaa atgcgaggca taccgctcga
    55021 cacgctacgc caaaaagcct acgaaaaagc gatggcgtat caaacggtgg ctgctattgt
    55081 agcagggcaa cggcaaggat acgaagaccg cctagaacaa gcggaaacct tagagcaaat
    55141 acaggcgatt gagccgatgt atcaattacc gcaaggagca aacaatgagc aaactgacta
    55201 aattaaaatc gtggagctac cacgtcttaa ttgcaataga tcaactcttt aacgctctca
    55261 ccggtggagc agcggacgaa acattttcta gccgttgcta ccgtggtgca gtgcttgcgg
    55321 aaaatccgaa aaaacgttgg cgtttttggc actctttttt gaattgctta tttttagacc
    55381 caaagcactg ctacgccgct tataaagcgg aggtacaccg caagcagtat ccgaaggcat
    55441 ttagacttgg ggggatttaa tgaaaaagca aaacttaaaa ttagtgcggg gcgatgatgt
    55501 gagttttaga ttcagcatta aagacagcac cggacaacct cttgcgttaa aggatttgtg
    55561 gtttgatttg cacttagtca gctatcaagg tgaggtgctg attgctctct ctactgatga
    55621 tggcagtatt gagacagcag aggctggtca gatggtgtta cattttagtc accagcaaac
    55681 tgctcgagct gaatggacaa gtgccaatta tgacttgcaa ctcacgcacg actatacaag
    55741 agtctaggag gtggagatga cctctatcat tgaactagac ctgactgatg acctcatcat
    55801 tgaggtgcag ttagatagca caatcattac cggcgatagc tttttagcca acctgccgga
    55861 tttactccaa atttacaacc aagcaaagtc agatagcagt aaggagctta acaatgacta
    55921 aacacaaaaa ccacgaaaac cacccgattg ctataacatt ctaccaactt ggcaaagatg
    55981 tcggcgacat taaccgccgg ctagcggtta ttgagcaacg tgagcaatca gcacagccaa
    56041 gccaaccaca agctgaggcg gttacaatgc cacagcaaga tgctgttgcc attaataaca
    56101 tcaatgccgt tttggaggcg gtcgaaaaat acaaaacttt tgaaaaatac aacactcagt
    56161 ggcaagagcg ttttgaaaaa ttcctgtctc gtaacccgaa tgattacgag ttaagtgaag
    56221 agcaacgcca aaaactttac cacacgatgt gccagcattt gtggcaggtg aactttactg
    56281 aaatcaatag taaacagtat gaattaccga tcccgcaaca attccagggt aagtttggct
    56341 atattgtcaa aacctcgggc ggtagcacag aaattagttt tgagttgcta tctgaaactc
    56401 acagcactta tctttatccg gatagctata ttcccgaggg cggtggcgat gtaatgctca
    56461 agccggctta tatttattta gtgattccgg acttagtggc aatgcgtgag ccggaggtgg
    56521 tgggggtttc accgctgaaa ctgaacattc tgcagaaaca cctgctgaca cctcagaaac
    56581 tacaggagca gaagcccttg aacacgaagg caaactgatt gatggcgtat ggcattttga
    56641 gcgagagccg gtaaatccac cgagagatca gaattattat gtggcaggta acctgttcta
    56701 caacggcata ccggattgat aaattgatgt ttcagtaaac ctaattaggg gggaatcccc
    56761 cttttttatt attataagga aagaaaatga ataaatcttt agtagcgtta gctgttgcag
    56821 cgatggcatc gaaagcacaa actaaa
//
LOCUS       DANLUA010000003        42573 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLUA010000003 DANLUA010000000
VERSION     DANLUA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42573
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1607
                     /gene="pckA"
                     /locus_tag="PXB44_000151"
     CDS             <1..1607
                     /gene="pckA"
                     /locus_tag="PXB44_000151"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL5280677.1"
                     /translation="LNRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKGV
                     LTNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLKT
                     LVTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFKP
                     DFVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYFL
                     PLKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFNY
                     EGGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPIY
                     HIDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGI
                     TEPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTR
                     GIIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDLA
                     GRFVKNFEKYATNEEGKALIAAGPKL"
     gene            1700..2701
                     /gene="bioB"
                     /locus_tag="PXB44_000152"
     CDS             1700..2701
                     /gene="bioB"
                     /locus_tag="PXB44_000152"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL5280678.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2861..2958
                     /gene="ffs"
                     /locus_tag="PXB44_000153"
     ncRNA           2861..2958
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXB44_000153"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2993..3700
                     /gene="gloB"
                     /locus_tag="PXB44_000154"
     CDS             2993..3700
                     /gene="gloB"
                     /locus_tag="PXB44_000154"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL5280679.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3859..4572
                     /gene="uppS"
                     /locus_tag="PXB44_000155"
     CDS             3859..4572
                     /gene="uppS"
                     /locus_tag="PXB44_000155"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL5280680.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4596..5465
                     /locus_tag="PXB44_000156"
     CDS             4596..5465
                     /locus_tag="PXB44_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL5280681.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5469..6779
                     /gene="rseP"
                     /locus_tag="PXB44_000157"
     CDS             5469..6779
                     /gene="rseP"
                     /locus_tag="PXB44_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL5280682.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6894..9275
                     /gene="bamA"
                     /locus_tag="PXB44_000158"
     CDS             6894..9275
                     /gene="bamA"
                     /locus_tag="PXB44_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL5280683.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTKNGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9355..10146
                     /locus_tag="PXB44_000159"
     CDS             9355..10146
                     /locus_tag="PXB44_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL5280684.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10146..11171
                     /gene="lpxD"
                     /locus_tag="PXB44_000160"
     CDS             10146..11171
                     /gene="lpxD"
                     /locus_tag="PXB44_000160"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL5280685.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11200..11658
                     /gene="fabZ"
                     /locus_tag="PXB44_000161"
     CDS             11200..11658
                     /gene="fabZ"
                     /locus_tag="PXB44_000161"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL5280686.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11692..12486
                     /gene="lpxA"
                     /locus_tag="PXB44_000162"
     CDS             11692..12486
                     /gene="lpxA"
                     /locus_tag="PXB44_000162"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL5280687.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12524..14524)
                     /gene="mnmC"
                     /locus_tag="PXB44_000163"
     CDS             complement(12524..14524)
                     /gene="mnmC"
                     /locus_tag="PXB44_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL5280688.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14749..16185)
                     /gene="trpCF"
                     /locus_tag="PXB44_000164"
     CDS             complement(14749..16185)
                     /gene="trpCF"
                     /locus_tag="PXB44_000164"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL5280689.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16273..16584
                     /locus_tag="PXB44_000165"
     CDS             16273..16584
                     /locus_tag="PXB44_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL5280690.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16738..17466
                     /gene="truC"
                     /locus_tag="PXB44_000166"
     CDS             16738..17466
                     /gene="truC"
                     /locus_tag="PXB44_000166"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL5280691.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            17484..17648
                     /locus_tag="PXB44_000167"
     CDS             17484..17648
                     /locus_tag="PXB44_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL5280692.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            17641..18117
                     /locus_tag="PXB44_000168"
     CDS             17641..18117
                     /locus_tag="PXB44_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280693.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(18154..19005)
                     /locus_tag="PXB44_000169"
     CDS             complement(18154..19005)
                     /locus_tag="PXB44_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL5280694.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            19066..20475
                     /locus_tag="PXB44_000170"
     CDS             19066..20475
                     /locus_tag="PXB44_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL5280695.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            20521..20871
                     /locus_tag="PXB44_000171"
     CDS             20521..20871
                     /locus_tag="PXB44_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5280696.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            20864..21187
                     /locus_tag="PXB44_000172"
     CDS             20864..21187
                     /locus_tag="PXB44_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5280697.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            21295..21774
                     /gene="smpB"
                     /locus_tag="PXB44_000173"
     CDS             21295..21774
                     /gene="smpB"
                     /locus_tag="PXB44_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL5280698.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            21977..23284
                     /gene="serS"
                     /locus_tag="PXB44_000174"
     CDS             21977..23284
                     /gene="serS"
                     /locus_tag="PXB44_000174"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL5280699.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(23343..23669)
                     /locus_tag="PXB44_000175"
     CDS             complement(23343..23669)
                     /locus_tag="PXB44_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280700.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(23662..23874)
                     /locus_tag="PXB44_000176"
     CDS             complement(23662..23874)
                     /locus_tag="PXB44_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280701.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            24061..26646
                     /gene="leuS"
                     /locus_tag="PXB44_000177"
     CDS             24061..26646
                     /gene="leuS"
                     /locus_tag="PXB44_000177"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL5280702.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            26846..27334
                     /locus_tag="PXB44_000178"
     CDS             26846..27334
                     /locus_tag="PXB44_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280703.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            27377..28402
                     /gene="holA"
                     /locus_tag="PXB44_000179"
     CDS             27377..28402
                     /gene="holA"
                     /locus_tag="PXB44_000179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL5280704.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(28403..28588)
                     /gene="yacG"
                     /locus_tag="PXB44_000180"
     CDS             complement(28403..28588)
                     /gene="yacG"
                     /locus_tag="PXB44_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL5280705.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(28570..29205)
                     /gene="coaE"
                     /locus_tag="PXB44_000181"
     CDS             complement(28570..29205)
                     /gene="coaE"
                     /locus_tag="PXB44_000181"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL5280706.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(29241..29897)
                     /locus_tag="PXB44_000182"
     CDS             complement(29241..29897)
                     /locus_tag="PXB44_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL5280707.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(29900..31096)
                     /locus_tag="PXB44_000183"
     CDS             complement(29900..31096)
                     /locus_tag="PXB44_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL5280708.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(31093..31545)
                     /locus_tag="PXB44_000184"
     CDS             complement(31093..31545)
                     /locus_tag="PXB44_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL5280709.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(31566..32045)
                     /gene="ppdD"
                     /locus_tag="PXB44_000185"
     CDS             complement(31566..32045)
                     /gene="ppdD"
                     /locus_tag="PXB44_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL5280710.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            32226..33605
                     /gene="radA"
                     /locus_tag="PXB44_000186"
     CDS             32226..33605
                     /gene="radA"
                     /locus_tag="PXB44_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL5280711.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(33660..33890)
                     /locus_tag="PXB44_000187"
     CDS             complement(33660..33890)
                     /locus_tag="PXB44_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL5280712.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(34010..34504)
                     /gene="folA"
                     /locus_tag="PXB44_000188"
     CDS             complement(34010..34504)
                     /gene="folA"
                     /locus_tag="PXB44_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL5280713.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            34580..34735
                     /locus_tag="PXB44_000189"
     CDS             34580..34735
                     /locus_tag="PXB44_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL5280714.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            34732..35700
                     /gene="proB"
                     /locus_tag="PXB44_000190"
     CDS             34732..35700
                     /gene="proB"
                     /locus_tag="PXB44_000190"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5280715.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            35700..36464
                     /locus_tag="PXB44_000191"
     CDS             35700..36464
                     /locus_tag="PXB44_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL5280716.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            36467..37099
                     /gene="nth"
                     /locus_tag="PXB44_000192"
     CDS             36467..37099
                     /gene="nth"
                     /locus_tag="PXB44_000192"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL5280717.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            37296..38546
                     /locus_tag="PXB44_000193"
     CDS             37296..38546
                     /locus_tag="PXB44_000193"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL5280718.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(38647..39582)
                     /locus_tag="PXB44_000194"
     CDS             complement(38647..39582)
                     /locus_tag="PXB44_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL5280719.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(39711..39905)
                     /gene="iscX"
                     /locus_tag="PXB44_000195"
     CDS             complement(39711..39905)
                     /gene="iscX"
                     /locus_tag="PXB44_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL5280720.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(39905..40246)
                     /gene="fdx"
                     /locus_tag="PXB44_000196"
     CDS             complement(39905..40246)
                     /gene="fdx"
                     /locus_tag="PXB44_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL5280721.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(40265..40630)
                     /locus_tag="PXB44_000197"
     CDS             complement(40265..40630)
                     /locus_tag="PXB44_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL5280722.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(40640..42493)
                     /gene="hscA"
                     /locus_tag="PXB44_000198"
     CDS             complement(40640..42493)
                     /gene="hscA"
                     /locus_tag="PXB44_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL5280723.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT        13019 a         8943 c         8483 g        12128 t
ORIGIN      
        1 tgttaaatcg tattgaacaa gaacttgcag acttaggcat tacaaatgta aaagaaatcg
       61 tgcgtaaccc tagctatgag caactgtttg aagaagagat gaaatcggat cttaaaggtt
      121 atgaaaaagg cgtcctaaca aatttaggcg cagtagcggt agataccggc attttcacgg
      181 ggcgttcgcc gaaagataaa tatatcgtat tagatgaaac gacaaaagat accgtctggt
      241 ggacatctga tgcagctaaa aatgataata aaccgatgac tcaatctact tggcaaagtt
      301 taaaaacctt agtgaccgat gagttatcta ataagcgttt atttattatt gatgctttct
      361 gtggtgcaag ccctgagcat cgcatcaatg tgcgtattat tacccaagtt gcgtggcaag
      421 ctcactttgt gaaaaatatg tttattcgtc caacagaaga agaattaaaa gatttcaaac
      481 cggattttgt ggtaatgaac ggttcccaag taactaaccc gaactggaaa gagcaaggct
      541 taaattcaga aaactttgtg gcatttaata tcacagaagg tgttcagatc attggcggta
      601 cttggtacgg tggtgagatg aaaaaaggta tgttctctat gatgaactac ttcttaccat
      661 taaaaggtgt cgcttcaatg cactgttcag ccaatgtggg cgaagaaggt gatgtggcgg
      721 ttttctttgg tttatcagga accggtaaaa cgacactatc aaccgatccg aaacgcaaat
      781 tgattggtga cgatgaacac ggttgggacg aaaacggcgt atttaactat gaaggtggtt
      841 gctatgcgaa aaccatcaac ctttctattg aaaacgagcc ggatatttat cgtgcaatcc
      901 gccgtgatgc gttattagaa aacgtggttg tgcgtgaaga tggcactatt gattttgatg
      961 ataaatcaaa aacagaaaat acccgtgtga gctatccgat ttatcatatt gataatattg
     1021 ccgtgccgtc aaaagcagga cacgctaaaa aagtgatttt cttaacggca gatgcattcg
     1081 gcgtattgcc accggtgtct aaattaacgc ctgaacaaac taaatactac ttcttatcag
     1141 gctttaccgc aaaattagca ggtacagagc gtggtattac tgagccaacc ccaactttct
     1201 ctgcttgttt cggtgcggcg ttcttatcac tccacccgac acaatatgct gaagtgttag
     1261 tgaaacgtat ggaagcagcc ggtgcggaag cctatttagt gaataccggt tggaacggca
     1321 caggtaaacg tatttcgatt aaagatacac ggggtattat tgatgccatc ttagacggct
     1381 caattgagaa agcggaaatg agtacattac caatcttcaa tttagcaatt ccaactgcgt
     1441 taccgggtgt agaaacccac attttagacc ctcgtaacac ttatgaagac aaagcacaat
     1501 ggcaagctaa agcagaagat ttagctggca gatttgtgaa aaactttgaa aaatatgcga
     1561 ccaacgagga aggcaaagca ttaattgctg ctggtccgaa gttataagtt gtaattataa
     1621 gttataatcg ctaaaaaatt agtttaggac taaaccttga atgaggttta gtccttttgt
     1681 ataaaggaga agtacaataa tgacaacatt ccaattaagg ctgaaagcca aagatgagct
     1741 taccccacac ccaacagcac aatattggcg aaaatgccaa gtggaaggac tatttgatct
     1801 gccgtttatg gatttagtgt ttagagcggc acaagttcac cgtgaaaatt ttgatgctaa
     1861 tgcgatccag ctttcaacgt tactctcaat taaaacagga ggctgtccgg aagattgcga
     1921 atattgcccc caatctgccc gctatgatac gggaatagaa aagcaaacaa tgcttgatgt
     1981 agagacgatt gtagaaaaag ctaaaattgc caaagagcgt ggagcaagcc gcttttgtat
     2041 gggggctgcg tggcgaggac ctaggcctaa agatattaaa gtagtctcag aaatcatcaa
     2101 tgcggtgaaa tcattggggc tagaaacctg tggtactttc ggtatgcttg aagagggaat
     2161 ggcagaagaa ttaaagcaag cagggctgga ttactacaac cataatttag ataccgaccc
     2221 tgaacgctat aacaaaatta tccatacccg aaatcacgaa gatcgaatgg acactttagg
     2281 taaagttcgc aatgccggct tgaaagtttg ctgtggcggt attgtgggaa tgaacgaaac
     2341 ccgccccgag cgagcaggcc taattgcaag cctcgctaac ctcgatcctc aacctgaaag
     2401 cgtaccgatt aatcagttga ttaaagtaga aggtacaccg cttaccaatg ccgaagattt
     2461 agactggaca gaatttgttc gtaccattgc ggtagcaaga attactatgc ctcaaagctt
     2521 tgtccgactt tctgctggca gaactactat gccggaggca atgcaagccc tctgttttat
     2581 ggcaggagca aattctattt tctatggcga taaattactt actacagaaa atccggaaga
     2641 agatcatgat cgccaattaa tggataaatt agatttatat ccgctacaat atatagcctg
     2701 agtgccaatg attttcgcct gaatgaaaat taaagaatca atcgttattt tacgcaaatt
     2761 tctaacataa aataaccgct tgcaaaattc gttcttgtct tatttttaaa tttatctata
     2821 atgtgaggcc ttacgcaagc ttgcgtaatt cgatggggcg ttattggttc ctcgcaatgg
     2881 tgttcagctt acttgatcag gttcggaaga aagcagtctg ggttgaaatt tccgtgtgcc
     2941 gagggaggct ggtaatgccc cacccttttt tcagttctat caggagaacg ttatgctcca
     3001 gatcaccccc attccggcat taaaagataa ttacatttgg gcaatccgta acgggcaaga
     3061 tgtgattatt attgatccat cagaacatca gccagcatta aactttattg caaaaaatgc
     3121 gttaaattta accgctatct tgattaccca taatcaccac gaccatactg atggggtgag
     3181 tggtattgtt gagcaatatc cgaatatccc tgtttatggt ccgcaagaag tggctgaatt
     3241 tgccaatgtg attgtctcgc ctgaacaaca tatcagcctg tttggttatg atgtaaggat
     3301 tattgaaagt ggtgggcata ccgcagggca tatcagttat ttatttggct atgattatct
     3361 gttttgtggc gatgcgttat tctccggcgg ttgcgggcgg gttttcaccg gtgattacca
     3421 agcccaattt gatgcgttac aacgctttaa aaatctgcct aactcggtta aagcctaccc
     3481 ggctcacgaa tacaccaaga gcaatttgaa atttgcagaa gcggttctac caccaagctg
     3541 tgtattatcg gaatatcaag aacaggttga tcttttacgt tcgcaaaaca aaccgacttt
     3601 gccaaccact attggcttgg aaatgcaggt gaacccgttt atgcaagcgg tcgatttagc
     3661 cgaatttatt gcacttcgtc agcaaaaaga caatttttaa cctcatattt ctaattaagc
     3721 attaaagcct ctcactttaa tgctttttgg ttaaataaaa cctttgcaaa catcgcttgt
     3781 caattccact ctggcttatc ctatggtata gttacgacta tttttagctt aatttattat
     3841 tttagtgagt aaaattctgt gaacttcgat cctcaaaata tgcctaagca cgttgccatt
     3901 attatggatg gcaatggcag atgggctaag cagaaaggaa aattacgcat ttttggtcat
     3961 cagaatggtg taaaggcagt tcgttctgca gttagttttg cagcaaagca tcatatccaa
     4021 gttttaaccc tctacgcatt tagcagtgag aattggagca gaccagaaac tgaagtttca
     4081 gctttaatgt ctctgtttat gaaagcttta gattctgaag ttaaacaatt acataaaaat
     4141 aatattcgct taaagattat aggcgataaa tccgccttta gcgaaaattt acaaaaacgc
     4201 attgccgaat cggaaaaatt aaccgaaaac aatactgggc taatcttaaa cattgctgcc
     4261 aactatggtg gttactggga tattgttcaa gctgctcaac aactagcagt gcacgttaaa
     4321 tctgaacaac ttgctgtgga gcagattaca ccagcattgt ttcaacaaca tttagtgaca
     4381 gaaaaccagc cgcaagtgga tttattaatt cgaaccagtg gtgagcagcg gatcagcaat
     4441 tttctattat ggcaaattgc ttatgccgag ctgtttttta gcccggtttt atggcctgat
     4501 tttaatgatg aggcttttag tgatgcaatt tatgcttatc agcaacgtga acgccgattc
     4561 ggtggttgtt aatataaaaa gaataaggaa atacaatgct taaagaacgt gtactttcag
     4621 cgattattat gattattgcc gtgctggctg ccatattttg gctctcacca cttcctttaa
     4681 ctttagtgtt atcggcaatt gttgtggctg caatgtggga atgggctcaa tttgccggct
     4741 ttaaacaccc tgtgccacgt gcaattgtgg caatggtgac aatctgctta ttactttttg
     4801 cgatcgttgc gaatactgat tatatccgag ccacccgttt tattactgat gagactaccc
     4861 ctatcctctt tattggctgt atttggtggt taattgccct tgtcctagtg gcaacttacc
     4921 ccaactcggc aaaactgtgg gaaaaatctg ttgcagctaa attcctattt ggacttgcta
     4981 ccttagtgcc gtttttaatt ggcacgctcg cattgcgttt ccataattat gcatttgatc
     5041 cataccaagg aacttattta ttcctttacg tctgtttatt agtttgggga gctgattcag
     5101 gtgcttattt tgctggcaga gcctttggca aaagaaaact tgccccaaaa gtatctccgg
     5161 gtaaatcgtg ggaaggcgta attggtggcg tactcacctc tagtttgatt gcctttacct
     5221 ttttacaact tacaccggca aatgtattcg gcaaagcatt agccactacc ccatttattt
     5281 tagtttcggt cggtatggtt gctatttcag tcttaggcga tttggcagaa agtatgttta
     5341 aacgccaagc aggcattaaa gacagtagcc agttaattcc tgggcatgga ggtattttag
     5401 atcgtattga tagtttaacc gctgccgttc cgttttttgc tacattcttc ttttttgtac
     5461 tatagaatat gacgtccatc attgcatttt ttattttaat ttgcgtactg gtttttgtgc
     5521 atgaatacgg gcacttttgg gctgctcgca aatgcggggt aaaggtgatc cgtttttcga
     5581 tcggatttgg taaagtctta tttaagaaaa aagataaaca aggaaccgaa tttgtttttt
     5641 cacttattcc tctgggcggc tatgtacaaa tgtggaatga tgaaacggat atcaacgctc
     5701 cagctcaaca agctcttaac acaaaatctg tattgcaacg tgcatttatt attatagccg
     5761 gtcctgctgc aaattttatt tttgcgattt tggcatactg ggtcgttttt attgccggta
     5821 ttccaactgt taaacctgtt attggggaaa ttttaccaaa cactatcgcc tcccaagcaa
     5881 gaattcctac cgaattggaa attacaaaaa tcggtcatca atctatccaa gattgggaaa
     5941 gtgcatcttt agcactagtt ggttttgtgg gcaataaaaa tgtaccgtta gagggaaata
     6001 tcaacggcaa ctcaacacaa aaatatgaac tcgatttatc taactggagt attgactcag
     6061 ccaaagaaaa cccattaacc gcattaggta ttcgccccaa aagtgcaaaa gtcaccgctg
     6121 aaattaaaca agttgtcgaa aattctcctg caagccaagt gggtattcaa gcaggtgatc
     6181 gtattcttca aattaaccag cagccattta attggttcaa tttagttgaa ctggtacaag
     6241 caggtaaacc tattgaactt aaaattgaac aacgaggtca gattaaagac cttgtcgtcc
     6301 aaccagaaaa aaaagacgaa cgctatatta tcggggtaat accaagttat gagcctcttg
     6361 cagataaata tcgtaccgaa ttaaaatatg atattcttac cgcattttat aaaagtatcg
     6421 aaaaagtgtg gtctcttacc caaaccatac tccaatttat cggtaattta ataagcggtg
     6481 atttgtccat taaaaattta ggtgggccaa tttcaatggc aaaaggagcc ggtgcaacag
     6541 ctgaaatcgg cttagtttat taccttagtt ttatggctct aatcagtgtc aatttggggg
     6601 taatgaactt atttccactt ttacccttag atggtgggca acttgtttta ttggctgttg
     6661 aagcaattcg tggaaaagca ttatcagaaa aaatacaact caaattccaa caaattggat
     6721 ttgcattcgt gctgagttta atgttattcg cctttgccaa cgatataatt cacttttaac
     6781 aagcggttaa aaaaggtgac ttttttgcaa aaagcaccct gacatcaaaa tccataatat
     6841 ggatttaaac tatattctta ataattttgc aaaacgatca taggataatt tcaatgaaaa
     6901 aattattact ttcctctctt ttaattgcaa acggtgtagt tgctgcacct ttcgtagtaa
     6961 aagacattcg tgttgaaggt gtgcaaccta caacaggcga atctatcatt tcatctattc
     7021 cggttagagt aggtcaaaca gcaacggata ctgacgtatc taatgtcgtc cgccatctat
     7081 tcagccaaca acgttttgca gatgttcgtg caacacgaga aggcaatacg cttgtcatca
     7141 aagtcgctga aaaaccgatt attggcaagg tagagatcga aggtaaccaa gcgattccta
     7201 aagatgcctt agagcaaaat ttaaaggcaa acttaattaa ccaaggtgaa atttttgatg
     7261 ctgccaagct tgaagctttt aaagaaggac taatcgaaca ttatcactct accggccgtt
     7321 atgaagccaa aattgacaca tctgtttcga ataacaccga aggtggcgtt aatattaagt
     7381 tagcgattaa tgaaggtgaa gttgctaaag ccaaaacaat taagtttgaa ggtaataatg
     7441 ccttctctga taaaaaatta ttagaaaact tagatattca acctgatgtt tcttggtgga
     7501 atatttttga aagtagcaaa ttcgaacaaa ccgcctatca aaaagactta gaaacattaa
     7561 gagattttta tttaaatcgt ggctatgcaa aatttgctat ccaagatacc gatgttcaat
     7621 tcaatgataa taaaacggaa gttgcattga cctataaatt agatgaaggg tcacagtacc
     7681 atgtcagtga aatgcgcatt atcggtaata cagccaatat ggatagccag ctcaacgccc
     7741 tactaaaaga attcaagcct ggtcaattat tccgtaaatc agaccttaca caaattgaag
     7801 aagagattaa atctatttta ggggacgcag gttatggttc agctaaagta gatatttatc
     7861 ctcaatttaa tgaagaaaat aaaaccgtaa aaatcagttt tgttgtggat gccggtcgtc
     7921 gtatttatgt tcgtaaaatc cgttttgaag gtaatggtgt cactgcagat tcaacattac
     7981 gtcgtgaaat gcgccaacaa gaaggtgctt ggctttccac aaacaaagct tctctaggta
     8041 aagctcgttt agaaagaacc ggtttctatg aaagtgttga tatgtcaatg ccaaatgtag
     8101 aaggtacagc cgatcaagtt gatgtagtgt ataaaattaa agaacgcaat accggtagta
     8161 ttaactttgg tatcgggtac ggcacgaaaa acggtatcag ctatcaagca ggaattaaac
     8221 aagataattt cttaggtatg gggtcaacta tcagtctaaa tggtacccgt aataactacg
     8281 gtaccagcgt taacctaggc tataccgaac cttactttac taaagatggt gtaagtttag
     8341 gcggtaatat tttctatgaa gattatgata actcagacaa tgattcaatg gcttcatata
     8401 aacgccaaac ctatggtatt aacggcacat taggcttccc ggtaaatgaa aataactcat
     8461 actacttagg tttaggctat acgcacgata aaattaaaaa tgcagcacgt gagtacactc
     8521 gtgagaaata tgtaaaatca atgaactttg agttcgatcc agctgccgat tattacaaaa
     8581 aaatcaaagc tgatgatttt gatttttctc tcggttggaa ctacaacagc ttgaatagag
     8641 gctatttccc gactgaagga actaccgcaa acattggcgg taaaatcaca attccaggct
     8701 ctgataataa atattaccga gtcagtgcag actttagaaa ttattacccg ttaaaccgtg
     8761 agcacaaatg ggtaatttca acgaaagcta gcattgctta tactaatgga tttggtggta
     8821 aagaagttcc gttctatcag ctttatactg ccggaggtat tggtacacta agaggtttct
     8881 cttacggtgc agtagggcct caagcgattt actataatac aaaatccggc aaatttgata
     8941 gcatgaataa tgatattatc ggtggtaatg cgttagctgc ggcaagcttg gaattaatca
     9001 caccaacgcc atttgtgagc gaaaaatatc agcacaatgt aagaacctct ctctttgttg
     9061 atgctgcaag tgcatggaat acaaaatgga agaaagatga atatacgaac ttgcctgact
     9121 atggtgattt caaacgtttc cgtgcttccg ccggtattgc attccaatgg atgtctccaa
     9181 tcgggccatt atcattctct tatgcgaaac caattcgtaa atatgataat gatgatattg
     9241 aacaattcca gttcaacatt ggtagttcat tctaattaac cacaagcggt taataaatgt
     9301 aaaaaatttg caacttagcc gcttatttca aaactgttca tacaaggaaa acaaatgaaa
     9361 aaattattta aaatgactgg cttagccgta gctttagcct ctacaaccgg aatcgcgaat
     9421 gctgcagata ctattggttt cgtagatcca agctatgtat tacaaaacca ccctgtatta
     9481 ttagatgctt cggcaaaatt tgataaattc atgaaagaaa gccaagaaaa atttgctgct
     9541 gatgaaaaaa aattagcaga tgaaaataaa accttaacgg ctgagcgcaa taaaattaat
     9601 gaagatgcgc aaaaattaca aaatgaacaa aaaaatgtag aagcttctat caagaaaaaa
     9661 gtggcagcat tagagaaaga tgcgcctcgc ttacgttcaa aagagattca agcacgtcaa
     9721 aatgcgattc aaaaagaagc aaacgcattc caagctaaag taacagcaat tcaaaaacgt
     9781 gaagctgatt ttgcaaaaaa agcagaagcc ttccagaaac gtgcagatga attccaaaaa
     9841 cgtattgaac aagcgaataa agaaaatggt ggcattaacc cacaagaagc gcaaaaacaa
     9901 gcggttgatg aagtaaatgc aaccattaaa gagatcgcaa aatcgaaagg ttacacttta
     9961 gtattacaac cacaagtagc actttatgct gaagatgaag gtaaagatat tactgaagca
    10021 gtacttgatg ctattgttaa aaaacatccg gaaattaaaa tagaaaaacc tgcaactgaa
    10081 gctcaaccag ctacagaaaa agaaaccaaa gctgaagaaa ctaaaccaga agaagcgaaa
    10141 aaataatggc aaatttccgt ttaattgatt tagcggagca tatcggcggt actcttaggg
    10201 gtaacgccga tttggctatt caaagtattg ctgctttaga taaagccgac agctcgcaaa
    10261 ttacctttat ctcaaatgca aaatatcggg aaaatttgac cgcttgcaat gctggagcta
    10321 ttatcgtcag cccggcagat gtagaatttt gccgagcaga tcaaaatctt atcatcgttg
    10381 ccaatccgta tgttgcttat gctcaactgg ctcaatatat ggattctact ccgaaagcag
    10441 cgagcgaaat ccacccaagt gcggtgattt cacctgaagc taaactgggt aataacgtat
    10501 cggtaggggc aaatgccgtg attgaaagtg gcgtagaatt gggcgatgat gccgtgatcg
    10561 gtgcaggctg ttttgtggga aaaaacagta aaatcggggc aagaacccaa ctttgggcaa
    10621 atgtttcggt gtaccacaat gtacaaatcg gagctgattg cttgattcaa tcctcaacgg
    10681 ttatcgggag tgatgggttt ggctatgcga atgacaaagg gcaatggatt aaaatcccac
    10741 aaaccggtgg tgtgattatc ggtaatcgtg ttgaaattgg tgcttgcact tgtattgacc
    10801 gaggtgcatt agatccaacc gtaatcgaag ataatgtgat tattgataac ctttgccaaa
    10861 tcgcccataa tgtacacatt ggttttggca ctgccgttgc cggcggcgtc attatggcag
    10921 gcagtctaaa agttggtcgc ttttgccaaa ttggtggagc aagtgttatc aacggtcata
    10981 tggaaatctg tgatggtgcc ataattaccg gaatgggtat ggttatgcgc ccaattacag
    11041 aaaaaggaat ttactcatca ggtattccat tacaaaccaa taaagaatgg cgaaaaaccg
    11101 cagcacttgt aatgaatatt gatgaaatga acaaacgctt aaaatcgtta gaaaaacgtt
    11161 ttaacgatta attttattag aggtcttaaa gtgacagaaa tgacaactga aaatcgtgag
    11221 ccaaatatta ttgaagtaac cgaaattatg gatatgttac cgcatcgcta ccctttcctg
    11281 ttagttgatc gcgtaaccga ttatgaagaa gggaaatggt taaaagccat taaaaatgta
    11341 accttcaatg aaccttgttt taccgggcat tttcctggcg caccaatctt cccaggagta
    11401 ttgattctgg aagcaatggc tcaagcaact ggcgtattag ccgttgccac ttatggtaag
    11461 ttaagtgaag acgaactcta ctattttgcc gcaattgaca atgctcgctt taagcgaccg
    11521 gttgtgccgg gcgatcaact tcaacttgag gttgaatttc ttaaagaagt acgtggtatt
    11581 actaaattta ccggtaaagc atacgttgat ggcaaattag tctgtgaagc agatttaatg
    11641 tgcgctcgcc gtaaataatt cgatataagc ccaaactaga gggaacaaac tatgcgtcta
    11701 attgattcaa cagcaaaaat tagcccgctt gccatcattg aagaaggtgc tgaaattggc
    11761 gctcacgttg aaattggtcc attttcagtc atcggtaaag atgtcaaaat cggggctaga
    11821 acgaaaattc actcaaatgt tgtcattaac ggcatgactg aaattggtga agataaccat
    11881 attttccaat ttgccagcat tggtgaaatc aaccaagatc taaaatacca aggtgaacca
    11941 accaaagtgg tgatcggtaa tcgtaaccgt attcgtgaaa gtgttactat tcaccgaggt
    12001 acggtacaag gcggtggcgt gacgaaaatc ggtgatgata acttatttat gatcaacacc
    12061 cacatcgcac acgactgctc tatcggtaac cgttgtatta tcgccaataa tggtacgcta
    12121 gcaggtcacg ttaccctaga tgatttcgtg attgtgggag gaatgtccgc cattcaccaa
    12181 ttcgttgtga ttggttcaca cgttatgctt ggaggcggtt caatggtcag ccaagacgta
    12241 ccgccttatg tcatggcaca aggtaaccac gcacgcccat ttggtattaa ctttgaaggg
    12301 ttaaaacgtc gtggctttga taaaccaact atgcacgcta ttcgcaaagt ctataaactg
    12361 atttactcca gcggaaaaac attggaagag tgcctagtag aaattgagca aattgcagca
    12421 acagaacctg cgattgctat ttttaaacaa ttcttcaaac gttctacccg tggtattatt
    12481 cgttaataaa cgaaaccccg aaatcaattc ggggtttgtt ttatcaagcg accgtcgaac
    12541 ctttcaacaa ttttcgaatc caagctcgat ttgggctaag gttatgccaa atatcctcac
    12601 tgattggcac aggctcattc acaatcaggc ttgctaacaa ttcccctaga agtggtgcag
    12661 tcgtcaaacc tcgagaagac aggccgttta ccatatacag attggtaaaa atttcagcat
    12721 tttctaccgc ttgtttccgt ctcaactggt tgtataaatt ggcatattgg cttttttgct
    12781 gctcaaaatg atgcactgcc ccaaccatcg gtacacgatc acgaaacgcc gcacgaatac
    12841 cgactttggc tttattttca gataaatcaa ttccttgcgt ccactcacaa acggttacat
    12901 tttgctgaag ttttgcaaca ttctcccaat gctccgataa actaaactcg gtttctgcat
    12961 tatcccgaat atggcttgct ccaatgcaat gcgtgccata ggctgacatt ggcgttaaat
    13021 aaccatcata acaaagcaca cacttcagtt tttgtaattc gcttgtagtc ggaatttggc
    13081 tcacttgccc tcgtaccgga tacaacggaa tgccgttcgc ttggctaaat tcattcaacg
    13141 tatgtccatt tgccagcaca accgcttgat gtcgaaccgt ttctcccctc gtctgccatt
    13201 gccacattcc attagaaaag gttaaatgct caactttgtg attcagtaca atcttcaacc
    13261 ctttctgctc cagccaagta aagccatttc taacaagttg taccggtgaa agccaaccag
    13321 cttgcggaat aaatgccccg ctgtttggca cgcttaaacc taatttttca cttaattctg
    13381 cctgtgaaca agattttaat aaggtgtcat cagccgtctg ctccaccatt ttttccagtt
    13441 ttttagccgt tttctcgtca taggcgtaaa gcactacccc gcttaaatta tgctcaaact
    13501 caatgttgga tttcagttgt tgcaagcgtt gcaatgcata atcaaaacta tggatataaa
    13561 actgtacatt tcgctcgtca tcatcgctta actgtgggta aatcgcccct tgtaaatttc
    13621 ctgaagcatt tattgccaag gtttcatctt tgcaatacag cgtgacttgc ttgccttttt
    13681 ctaacagcga taacgcaacg aagaaactcg ctactccgcc accaataatt gcaatgtcat
    13741 cagcggtttc atttttggca taaaaatagg ggtaattagg ctcggaaact tggctatcta
    13801 acggtttctc gccccaaagc atttctcgtt ttttgccaaa tcctttgcgt tttttcactg
    13861 caaaacctgc ggcttccagc cctcgtctga ccatacttga agcggtaaaa gtagcaaaac
    13921 tgccgccatt ggcacttagt cgaaacattt gcagatataa ctgttcgttc cacatttctg
    13981 ggtttttatc aggcgaaaag ccatctaaaa accaagcgtc aatcgtgccg ctataaagat
    14041 cgcctaattg gggtaagttt tcagcaatct caccgaacca taaatctaaa taaacctgct
    14101 caaaatgata acgttggcaa cctatttggc gaggctgcca acaagcggtt aaattttcag
    14161 ataatgttgc aaattgcgga tagcgttggt gaatatcagc taattggctg gccgtcagag
    14221 gaaatttctc aaatgagata aaatagagtc gttttagttt actttgcgga aatgctgccc
    14281 gaaactgttg gaatttgtca gcaacggcta aaaaattcaa gcccgtgcca aagccggttt
    14341 ccgcaataac aaaagcctct ttcgggtgag attgccactt ttcccacagt tggttacctt
    14401 cttgaaacac atagtaactt tccgccaaac catcttgggt agaaaaataa atatcatcaa
    14461 attgctccga caccggtgta tctgcatcat taaatgtaag tgtcgcaaaa gtcagtttac
    14521 ccatcatatc cccttaaaaa caaaacacgg aaaaccccaa gattttccgt gtttcaacca
    14581 gttcaatagt accaatgtta gaaaatgttg ctggcaaaaa taacgatcat accaagtgcg
    14641 cctacaaccg ttagcattga ataaccaaaa gtacccattt tacaatcctc attgttaatt
    14701 atgattaaaa cggctttatt ctagcataat ttccatcgct ttgaatgcct atgcttgaat
    14761 aattttgcca aaacaagcgg tcaatttttg taaatctttt acgcctttgg cactttccac
    14821 gccagaattt aaatctaacc cgacacagcc ttgagcaagg gcttgctcaa tattctcagt
    14881 gccaatacca cctgccaata acgctttttg ttttaaggtt tctggaatca acgcccaatt
    14941 aaaggttttg cctgtgccgc cttgctgatt gcctactttg ctgtctaata cataacgagc
    15001 aatctgtgga ttatcgttaa attctagatt ttcaacctca acatcaaccg ataaggcttt
    15061 ccagatttga gttttactac ctaatttctc tgccagttct gcaatgtatt gctcatcttc
    15121 cgagccgtgc aattgaactg caaataactc aagctgtttt gccatttttt ccacaaattc
    15181 gaccgcttga ttttggaaca cccccacata acgcaacggt gcattcacca ccaattcttg
    15241 ggcttggcga agtgaaagct ggcgaggcga gccttcggca aaaattaaac cgccatataa
    15301 tgccccttgc tcataaaccg ctttcacatc ttgcacacgg gttaagccac acactttgtt
    15361 ttcaccaaaa atcaccgccc gcaccgcatt attgagatcg gcacttccca ttaaactgct
    15421 gccgattaaa aaagcatgaa catacggctt aatgtttttc acttgctggt gatcgttaat
    15481 acccgattct gaaatcaaac agacatcagc cgaaatttgg gcttggtatt tttccaccaa
    15541 acgcacaatg cggttcatat caatcgtgag cgtgtgcaaa tcacggttat taacgccaat
    15601 taccttcgcc cctaaggcta aggcacgttc aaattccgcc tccgtgctgg tttcagtcaa
    15661 aacgcccatt cctaactcgt gagccagtgt ggcaagttca cggtaggttt catcatttac
    15721 caccgataac atcaataaaa tcgcatcagc attataaaaa cgggcaaggt aaacctggta
    15781 tgccgaaatc ataaaatctt tacacaaaat cggctgtgtg gtttggcgtt tgacttgatc
    15841 gatataacgg aaatcgcctt gaaaatattg ctcatcggtt aaaaccgaaa tcgccgcagc
    15901 gtaattttta tacacttggg caatactgtc taaatcgaac tcagcacgaa ttaacccttt
    15961 tgacggtgaa gcttttttac attctaaaat ataagctggt tcttggtgtg tgcctttggc
    16021 aagagccgca tagaaatcat ggtcgctttt tacaattttg tgctgaaatt cacttaacgg
    16081 aaaggcttgc tctttttgtg ccacccaaat ggctttatct tgtacgatct tttgtaaaat
    16141 cgtcggttgg tttggttgag cgttatcttg aacgatgttt tgcatataaa ttcctagagc
    16201 gaaatcggtt tgaatggtaa aatctgccct tatttttacc cgaaaaagtc tcatttcaca
    16261 agcaaaacta agatgaaatc acaagttata cagcatttat cttatttaga aatcgccctg
    16321 agagttaatc aactttggga ggcgacacca ccaagccctg aagccttagc caatgagcaa
    16381 cctttctgcg tggaaacctt aaccccaacc caatggctac aatggatttt tattcccaga
    16441 atgcgggcca ttttagatgc aaatgccgat ttacctcgca attttgcaat caccccttat
    16501 ttagaagagg cattgaaaaa cgaaggttat ttacaagcga tttgtgaacc gctattaggt
    16561 ttggaaaagt tgttgaaaga ttaacttctc tctcagttct tttattaatc tgacaaattc
    16621 gttgatagtg ccagcgtcca cgctggtact aattttaacg gttaattaat caaaatttgg
    16681 cacaagcgag gacgcttgcg ccatcataga gatttgataa attatactta atgtattatg
    16741 accttagata ttttatacca agacaactcg cttatcgcca tcaacaaacc tgccggagtg
    16801 ttggtgcatc gttcttggtt agataaacac gaaacggtgt ttgcaatgca aaccctgagg
    16861 gatcaaatcg ggcaacacgt ttttccgatt caccgccttg accgcccaac ctcaggtgta
    16921 ttgctgtttg ctctcaacag cgaaaccgcc cgaataatga gcgagcagtt tgaacaacac
    16981 ctagtacaaa aaacttattt ggcggtggtg cgtggctatt tacaaggttc ggcacaaatt
    17041 gattatccgc tcaaagtgat tttagataaa attgcggata aattttctca ggaaaaagag
    17101 gctcaagcgg cgattactga ttataaaggc ttagcaacgg ttgaaatgcc ttaccctgtc
    17161 ggcaagcacc aaacctctcg ttatagtttg gttgaactta gccctaaaac cgggcggaaa
    17221 catcagcttc gccgccacct gaaacacctg ttccacccga ttatgggaga cagcaaatat
    17281 ggcgacttac accaaaaccg tgctttagtt gaaaaatcag gcgtaagtcg tttaatgtta
    17341 cacgcccata aattgcaatt tcagcacccg aaaaatttgc aaaaaataga gataattgca
    17401 tcgcttgatg agcaatggca acgcctcttt gcattttttg actggaactt tactcaatat
    17461 tattagacaa aaaagaggat tgaatggata attcactttt atcattgaca cacgagcaac
    17521 aacaagccgc agtagaagaa attcaaaaac tgatggcaca aggcatcagt gccggagagg
    17581 caattcaaat tattgctaat caattacgtg acaaatatca aatattggag aaatcaaaca
    17641 atgaataaat tatggacagt gcttgcccta acaataactt cccactatag ctttgctgcc
    17701 gctgaaaaaa tagtcactaa agatttaccg gcagaaggaa aacctgcagt cgtgctacag
    17761 gtttttgatg aaagtgaaaa aattgcaaaa cctattaagg gaaataagct ctttattagc
    17821 aaaaaacaaa caaggctttg ctggcacagt attaatcttc ctgtacaaaa taaagttact
    17881 attgcagaag ctttctatgc ccctgctgca atgaaattag cttctcccgg ctctaaaatt
    17941 aattcttctg cagacaagaa aaatcatact atcattactg aaataaacct tgcaaataat
    18001 caagtattaa atcgttgctg gggttttgat aaaactgacc ctgtcggtaa atacaaaatg
    18061 gaaattcaaa ttaacgatca cattttcaaa ggtttagaat ttgaaattgt aaaataacct
    18121 aaaagccaca gagtaacacc ctgtggcttt ttgctaaatc attgttttta aattggttaa
    18181 attgtgcagc aaataatctt tactcttcac aaaggctctc gcctgttgcc agttgctaaa
    18241 ttgttgctga gtgcattgat taaaacgtac cagcacttcc aaactttcat tgccgacaca
    18301 aataaaatca gctaataatt gggttaattt ttcactgttt tcaccatcca ttgcagttgc
    18361 aattttctgc tcccatttaa ccgcttgtcc gagcaaataa ccccgctggg ctttgcttaa
    18421 attactcaac cacatcccat ccgcttttgg cattgcctca accaaagtaa ataagtcgcc
    18481 tagatttcct tgcttgattt caaactccaa ttcacaaatc ggctcttcac caaaatgatt
    18541 tttaatgatg ccctgatcta acgctacctc aatgtcagac atccccactt gcaccaacca
    18601 agttttacga gtgaaatcgg tgctgaatat cgcttgcaag ctttctgcaa tctgctctga
    18661 attttcaaac ggcaactggt atttttccac taatgtttga aaatccggct ggctattggg
    18721 taaagctaaa ttatattccg gacgaatatg caaaccgcct acaatttccc cttgtgtttt
    18781 taacgtgatt tcatattgtt gattcacaga acgcacccgc agccccattt tttgcgtggc
    18841 aaaaaattgc tcgggcgtat cataataggt gttgcctaac aattccgttt gatgagcaag
    18901 cacagggaat ggcgaggttt gattcaagta gttttccact aacgccacat tctgctgttc
    18961 caacattatt ttcagttcga tttctgcttg tttttggttt tccatttcac acgcccctgc
    19021 aacgttgtat aatcaagcgg ttattttatc aataaatttt gcaaaatgtt ttcaaaaatt
    19081 caccataaac tgaataactt tgtccagcgt ggactcgaca accatttacg cctcgccgtt
    19141 accggcttaa gccgtagtgg caaaacggca tttattacca gcttggttga tcagcttttg
    19201 catatcaaca aaggcggtaa ttcgcattta aacctgtttg cgcctgcacg ccacgggcaa
    19261 attctatcgg taaaacgcgt tgagcaaggg gatttaacta ttccccgctt tgaatatgat
    19321 aaaaaccgcc aaagccttga aaatcaacca cctcaatggc ctgaatccac cactggcatt
    19381 agcgaaattc gccttgcgat tcgctaccag cggcaacatt ctctgctacg tcatattaaa
    19441 gaaaccggca cgctttattt ggatattttc gattacccgg gcgaatggct gttagattta
    19501 cccctgctct ccctttccta taaagaatgg tcgcaaacgc aacaaaccat acataaagat
    19561 gagcgtgcag agcttgccac accttggtta caagcggtta aaaaactcga tctttttgca
    19621 aaaaccgatg aaaatttact ggcagaattc agccaacaat acaccgatta tttgctcgcc
    19681 tgcaaagctc aaggaatgca atatattcag ccggggcgat ttgtgttgcc aactgaaaat
    19741 gccaaaggtg caccggtatt ccaatttttt ccgttattag atttaagcga agctgagtgg
    19801 gaaaagttgg aacaaagcga cagaaacagt attttccata ccctcaaaaa acgctatgag
    19861 cattaccaga aaaaaatcgt caaaccgttt tatgaagatt acttctctca atttgaccgc
    19921 caagtgattt tggcagactg cctcacaccg ttaaattatg gctataacga atttatggaa
    19981 atgaaattag ggttacagca actgtttaaa catttccatt atggcaatcg ttcgctattt
    20041 caccgtctgt tttcgtccaa cattgataaa ttgctattta tcgcaacaaa ggcagatcac
    20101 attactagcg accaagtgcc gaatttggaa agcctaatgc gacaattagt gcaagaaggg
    20161 ggaagacacg cagaatttga tggtatcgaa accggctatt tagccatttc ctcgattcaa
    20221 gcaaccgatg cggtgaaagt gactcagcaa gataaaatca ttaaagcgat aagagggatc
    20281 cgttccagcg atagaaaaca agtgacactc taccctggca gcgtgccaaa tcgcataccc
    20341 gataaaaatt actggcaatt taaccgtttt gaattcgacc aattcgagcc caagcaactt
    20401 gattatagcc aagcgattcc acacttcaga atggattcgg tgctacaatt tttattggcg
    20461 gatatgtttg agtaaaatct tgttgcatat ttccattaaa ggtataaaat tttatacatt
    20521 atgaatactg taatttccca acgaaaacca attcactggg ttggatcaac cttagatgat
    20581 attcgtgaat ttccggataa cattaaaaga gaactcggtt ttgatcttga tttagttcag
    20641 caaggcttat taccgaggga ttttaaagcg atggcaaatt tgggttcagg cgtaatggaa
    20701 attcgagtaa aagatccctc aggggctttt cgcttagtgt atttagctaa atttaaagat
    20761 gcaatttact gtttacacgc tttccaaaag aaaacgcaaa aaacagcccc taaagacatt
    20821 gctgtcatta aagcacgcta tgctgccgta aaggagttag aaaatgaata atattgatac
    20881 taaaattcgc catactacag aagctcaggg taatgttttt gaagatcttg gtttccctat
    20941 tgatgaagct caaaaattga aatctgccag tcagcaactg attgaaacta aactgatgct
    21001 aatgaatgaa atgagcaact ggattgataa aaataatctc aaacaatcag aagcggcttc
    21061 tattctaggt gttagccgcc ctcgaatttc tgatatggtc aatggcaagt tagaaaagtt
    21121 taccctagat accttagtca cttttgttgc aaaaaccggc aaaacaatcc aattgcaatt
    21181 atcctgatat ttgcaaaatt ttactcaaat tcaaccgctt gttgctttta tcattcacta
    21241 acaaggttat ttcaagtaaa attgtgacta attttttatt aataaacaca aattatggca
    21301 aaaaaaccta aagttgcatc aaatacgatt gcgttaaata aacgtgccag acacgaatat
    21361 ttcattgaag atgaaattga agccggctta gagctacagg gctgggaagt caaagcattg
    21421 cgtgccggaa aaggcaatat cagcgatagc tatgtcatct ttcgccacgg cgaagccttc
    21481 ttattcgggg caatgattac gcctttgaat atggcatcaa cccatattgt cagcgacccg
    21541 acccgcaccc gcaaacttct actgaataaa cgtgagttag actcactgtt cggtaaagtt
    21601 aatcgtgaag gttataccat tgttgccacc tcaatgtatt ggaaaaatgc gtgggtgaaa
    21661 gtaaaaatcg gcttagccaa agggaaaaaa ctgcacgata aacgtgaaga tattaaagac
    21721 cgtgaatggc aagtggctaa acagcgtatt atgaaaaacg ccaatcgtgg ctaaatgaat
    21781 aattaccctc tccccttgtg ggagagggac agaaaaattg cgttcagcaa tttttcaggg
    21841 aaagggggat ttgtaaaagt tcaaggaaaa acaacgcttg ccctctctct gaaaaatatt
    21901 tctgaacgaa atatttttcg gtctctctcc cataaaggga gagagtgaaa cacaaattta
    21961 aactaaggaa catacaatga tcgaccaaaa cctacttcgt accaatttag aagacgttgc
    22021 tacagctctt aaaatcaaac gtaattttat tttagatgta gagcgagtaa aaacccttga
    22081 agaacagcgt aaaacgctac aagttaaaac cgaaacctta caagcagaac gcaacactcg
    22141 ctccaaaaac atcggtgcgg caaaagctcg tggcgaagat atttcagctt tattagccga
    22201 agtagattca atgggcaacg aattagacac ggcaaaagta gaattggata aagtacaggc
    22261 agaaatccgt gagttgttgc tttctgtgcc aaacttaccg gcagatgaag taccactcgg
    22321 taaagacgat agcgaaaact tagaagtatt acgttggggc gaaccacgtc aattcgactt
    22381 tgaagttcaa gatcacgtta cccttggcga aaacttaaac ggtttagatt ttgctgccgg
    22441 tgtaaaatta accgccagcc gttttgtggt aatgaaaggt aaacttgccc gcttacaccg
    22501 tgcattatct caatttatgt tggatttaca cactgaacaa cacggctatg tggaaaccaa
    22561 tgtgccattt ttagtcaatc acgacactct atacggcaca ggtcagctac caaaatttgg
    22621 cgaagattta ttccatactc aaccattaac agggcaagat ccaaacgagg tgcaacgccc
    22681 attcggtttg attccaaccg ctgaagtgcc agtaacgaac ttagtgcgag atgaaatcat
    22741 tgatgaagac agcttaccac tgcgttatac cgcccacacc ccttgtttcc gtgcagaagc
    22801 cggttcttat gggcgtgata ctcgtggttt aattcgtatg caccaatttg aaaaagtgga
    22861 aatggtgcaa attgtcgccc ctgaaaaatc aatggaagcc ttagaagaat taaccggtca
    22921 cgcagaaaaa gtcttacagc ttttaggctt gccataccgc aaagtgctac tctgcactgg
    22981 tgatatgggc tttggtgcag caaaaaccta cgacttagaa gtttggctac cggcacaaaa
    23041 cacttaccgt gagatttctt cttgctcaaa tatgtgggat ttccaagccc gccgaatgtc
    23101 tgcacgttgt aaagctaaag gcgacaagaa aactcgcttg gttcacacct taaacggctc
    23161 aggtttagcg gttggtcgca cactggtcgc tgtgttagaa aactaccaaa atgccgatgg
    23221 ctctattact gtgcctgagg tgttaagacc ttatatgggt ggtttggaag taattaccgc
    23281 ttaagattaa taaatgaaac caatctcaga aatggggttg gtttttttaa taaatcttat
    23341 ccctaatctt tttcaggata attcataaaa atttcagtaa ctactcggcg gttttgcact
    23401 gtatctaaac gaataggatt tttcattctc cctaaacgaa atgcaataat ggtgactttt
    23461 ttcggttcat ctattttatc taattgatag aataccatta tttcccgttg ataaaccgct
    23521 cgctttctga aacgtaaatc cttgcgaaat gcgacacctg cttttggata aaactgtaaa
    23581 ccattgatga tattcgtttg agtatgctcc gaaaataatc catagaaaat aaactctaat
    23641 agattatttt taaaatcaac actaaccatc taactgctcc caagcagtta aatctcctcg
    23701 tgataaacgt tcagcaatat cagcataaaa ttgtttcggt acttcccttt ttacttggta
    23761 ttgagctgat tgttcttcta ataaatgttc ttctatcatc ttgatttctt ctgttgtcca
    23821 ctcttcatct tctttatgta caacaaaatc ggtatctaca atcacaatag acatacctag
    23881 ctccttcaag tactggttat atattctgta ttttgcctgt tctaaattca gctgtctagc
    23941 ttttctgtta ctcacaattt gcaaattttt catcaaattc caccgcttgc cattcaaaaa
    24001 aaggcgtttt tgcggtatcc tatcggcaat tttttcagtt aaatttaaag gttaaatttt
    24061 atgcaacagc agtataaccc aagtgagatt gaacctaagg ttcagcgtta ttgggcagaa
    24121 aataaagttt ttaaagcagt taaagacgtt accaaagaga aatactattg cctttcaatg
    24181 ttgccttatc cgtcaggtaa gctacatatg gggcacgttc gtaactacac cattggtgat
    24241 gtagtgtctc gttatcagcg tatgatcggt aaaaatgtat tacagccgat gggttgggac
    24301 gctttcggtt taccggcgga aggtgctgcg gtgaaaaata ataccgcacc ggcaaaatgg
    24361 acttacgaaa acatcgaata tatgaaaggt cagcttaaaa tgttgggctt ctcttatgac
    24421 tgggatcgtg aagtcaccac ttgccgtccg gaatactaca aatgggagca atggttcttc
    24481 acagaacttt ataaaaaagg cttagtgtat aaaaaaacct caaccgtaaa ctggtgtccg
    24541 aatgatgaaa ctgtgcttgc caacgagcaa gttcacgaag gttgttgctg gcgttgtgac
    24601 accccagtgg aacagcgtga aattccacaa tggtttatca aaatcactga ctatgctgaa
    24661 gaattactca atcagttaga taatctccca cagtggccgg atcaagtaaa aaccatgcaa
    24721 cgcaactgga tcggtcgttc cgaaggggta gaaatcacct ttaaaattgc cggttcaaac
    24781 gtagaattac cggtttacac tacccgtccg gataccttct tcggcgtaag ctatgtggcg
    24841 attgctgcgg ctcacccatt agcagaatta gctgcacaaa ataacccggc attggctgaa
    24901 tttatccgtg aagcgaaaaa caccaaagtg gcagaagcag aattagccac catggagaaa
    24961 aaaggaatgg caaccggctt gttcgccatt cacccgttaa caggaaaaga agtaccggtt
    25021 tgggttgcta actttgtgtt aatgcactac ggtacaggtg cggtaatggc ggttccggct
    25081 cacgatgagc gtgactttga atttgcacaa aaatacgatt tgcaaattaa tcaggtaatt
    25141 caaccgcttg atggctcaga gtgggatttc agcaaagccg cttacaccga acacggtaaa
    25201 ttaatcaact ctgccgagtt tgacggcttg gaatttgatg tggctttcac tgcgattgcc
    25261 gataaattag aggcaatggg tgtaggtaaa cgccaagtga atttccgtct gcgtgactgg
    25321 ggtgtttctc gtcaacgtta ttggggtgca ccaatcccaa tgatgaccac agaagagggc
    25381 gaagtggtta ccgttccatt acaagattta ccggtgattc tccctgaaga tgtggtaatg
    25441 aacggcgtgc aaagcccaat caaagccgac ccagagtggg cgaaaaccac ttacaacggc
    25501 aaaccggcat tgcgtgaaac cgataccttc gataccttta tggagtcctc ttggtattat
    25561 gcacgttata cttgcccaca atatcacgaa ggaatgttag attcagacga ggcaaactac
    25621 tggctaccgg tcgatcaata tatcggcggt attgaacacg ccacaatgca cttgctctac
    25681 ttccgtttct tccataaatt gttgcgtgat gcgggtattt taaattcgga cgaaccggcg
    25741 actaaactgt tatgccaagg tatggtgctt gccgatgcgt tctactacac cagcccaact
    25801 aacgagcgta tttgggtcag cccgacacaa gtcaccttag aacgtgatga gaaaggtcgt
    25861 atcatcaaag caaccgatcc acaagggcgt gagctggttc acagcggtat gactaaaatg
    25921 tcgaaatcaa agaacaacgg tatcgatccg caagaaatgg tggaaaaata tggtgctgac
    25981 acagttcgct tgtttatgat gtttgcctcc cctgcggaaa tgacattaga atggcaagaa
    26041 tccggcgttg aaggggcaaa acgtttctta ggtcgtgttt ggaatttagt ttatagctat
    26101 gcccaaaatc cggcaacgac tgcattagat gtgaatgcct tagacaagga tcaaaaagaa
    26161 cttcgccgtg atgtacacaa aacgattgct aaggtgagtg atgatattgg tcgtcgtcaa
    26221 accttcaata cagcgattgc tgcggtgatg gaattgatga acaagctcac caaagcccca
    26281 cttgaaaccg aacaagataa agccgtaatg gcagaagctt taagtgcggt ggtgcgtatg
    26341 ctttacccaa tcacgccaca tatctgcttt gagttatggc aagtgttagg taatgaacaa
    26401 accatcgatt ttgccccttg ggtacaagcc gatgaatccg caatggtgga agatgaaaaa
    26461 ttagtggtag tacaagtcaa cggaaaagtg cgtggcaaaa tcactgtttc agctactgct
    26521 acggaagatg aagttaaagc tatcgctaaa gccgatccaa acgttgctaa attcttagat
    26581 ggtcagcaga ttgtaaaaga aatctacgtt ccatttaaaa tgttgagctt tgcggttaag
    26641 gcgtaacaaa cacactctcc cttatatggg agagagaccg ataaatattt cgtgcagaaa
    26701 tatttatcag agagagggta caagcggtta ttttcttgga aaattttgca aaaaatagcc
    26761 ccctctccct gctttttctt ctaaacgaag aaaaacctgt ccctctccca caaggggaga
    26821 ggggaataaa caaggaaatc tcattatgat taaaaaactc tctctcatcg ctgtttttgc
    26881 tcttaccgcc tgcggttggc actttaaaaa caatgaggta ttacctgaat cgttcagaac
    26941 attaaccttt gaaagtgcag atcaacacag cgaaatgtct cgaattttac gcaatcagtt
    27001 acaactaagc gatgtaaaat tagtgcctag cacggctaat gtggcaaaat tacgtttggt
    27061 ttcaacctca acagacagca aagtggtttc tgtgtttaaa caagctcgtg aagcagaaaa
    27121 aatcttaacg ctaaacgttg atgcgattgc cgatattcca aataaagggc aatacccttt
    27181 agaagtttcg gttcatcgta ctttctttga tgactctcgt gcagctttgg caaagtctgc
    27241 tgaaaaagag atgattatga aagatatgta tgaacaagcc tctcgccaat taattatcaa
    27301 aatggcgggc ttaaaaaaag aactctcaaa ataatcattg taggggcgaa atatgtttcg
    27361 cccattgttt atctgtatgc aaaaaatctt tccggaaaca cttcaatttt cattaaacaa
    27421 aggactacag cccttttatc tattaacagg gaatgatctg ctattggtga atgaaaccaa
    27481 agatgccatt attcacaccg cccgtctcaa cggttttgat gaaaaaaaag aagttgaaat
    27541 caaaaacgat actaactggg aagaactttt cgaagcagct caatcaatgg gattgttttt
    27601 tgcacgccaa attatggtgc tgaattttcc ggattccccc acagcgacac agtttaaaaa
    27661 actggctgag ctttgtgcct ttattcatag tgatttgctc tttatcctgc acttacctaa
    27721 attttccaaa acaatggaaa aacaggcttg gttcacgcaa attgagctaa atctcgtgca
    27781 aattaactgc caaacaccgg aacttagcaa acttccacaa tgggtcagtc atcgagcaaa
    27841 agcgatgaat ttgcaaattg aaaatgaagc aaaccaactc ctttgttata gctacgaagg
    27901 caatctactg gcgcttaaac aggcattaca actgctacaa ctcaatgcaa ctgatggaaa
    27961 aatcacgctg acaaaagccc aagaagtggt tgaacactcc gcccaattta ctccatttca
    28021 atggattgat gcattatttg aaggaaaaat ccaaagagct caacgcattt tgtaccatct
    28081 gcaaaatgaa gaagtacaag cggtcgtttt attaagaatt gtgcaaaaag agttgatgat
    28141 tttactcgaa atcactcgca acccaaccgc agtcgtttcc agcgaacaat cactttttat
    28201 cggtaattta cgaacagaat ttgaccgctt gaaaatttgg caaaaccgca gaccttttta
    28261 caaccagatc actcaacgac tgacttacca aaaactttac gccctaatcc aacaactggc
    28321 tgaattagag cgtaaggtga aacaagaatt tagcgatgat atttggcaag aattagagcg
    28381 tttttccgct caatttacct aactaatact tttctaaatc ttcgctaaac aactccgctt
    28441 cttcactggc gatagagtgc ttttctgccg cccaatcgcc taaatcaata atttgacaac
    28501 gctcgctaca aaacgggcgg tatttacttt ctttactcca caccacttct ttttcacaag
    28561 tcgggcagtt tacaatagtt tctggcattc tttttccttt tttgctaatg ctaagtattt
    28621 ataatgtaag gcttgaacct gctctttaat taaaagcaag ccctgttcaa atggcaagtt
    28681 attttcaatc acatcatcag cataacttaa tctttcttca cggctgactt gggctgccat
    28741 aatattttta atggtttcaa ttttactgtt atcacgtttg gttgctcttt ccagttggat
    28801 ttcaggagaa acatcaacaa ccaatatacg atcacaatat tcggtgagct tattttcaat
    28861 taaaagcggc acgacccaaa tcacataaca agcggtcgat tcttgcaatt tttttaccat
    28921 ttcttctcga atggcggggt gaagtaaatt attgagccac gttttttcct cttcattgtt
    28981 aaacacaatt tggcgaagtt tagtgcgttc taatgctttt tcagcggtta agatctcttc
    29041 accaaaatgc tctacaatct tggctaaaag cggtgtaccc ggttcaacaa cttggcgagc
    29101 caccacatca gcatcaatta cctctacccc aagagctgaa aataaatcag acactgtggt
    29161 tttgccactg cctattccac cggttaagcc tacaacgtaa gtcataaaaa ttgattcctc
    29221 aaaataaaaa caaaccaatt ctagtaatta atccctctgc tttcaagcat tagcaccagc
    29281 aacatcgctg tgctaataaa cggaataaac gccaattttg ctaatttctg ctttttcatt
    29341 agccaataag ccaagtaata accaatccct agcaggctgg cggttaatat caataagagc
    29401 attttttcga gccgaaagag tggggataac gcaaccaata acaacatatc gcccgagcca
    29461 aatgcctcct ttttaaacat aagctgtgaa accaagccaa tcaacaggca aaaagcaagg
    29521 gtaaaggtaa aagtttggtg ttggaaatcg gctaaactct cccataacac caacaaaaat
    29581 ataagcacga ggtagcgaat atccgttagg taatagcaca tatccagcag tgataaaaaa
    29641 cagagcagaa taaaaatcgt gccgataata gggctttccg agcagagcat aatcaatgga
    29701 aaaaggagaa aaaatagatt tgccgatcgg ggttgtttcg gttgtagcac cgattgagcc
    29761 aaaaactgtt gctcggttaa gccaatattc actaatgaag ttgtttcttg gtaaatttgg
    29821 cgattgactc gaatggcaaa tcgagggatc tccgctttga gccaaaccgc aatgaagagg
    29881 gaaaacagta ttaacatcgc tactccacca aagcccccat atcaaaaatc ggtaaataca
    29941 gcccaacaat aatagtgccg acaatcacgc ccattatcaa cattagcatc ggttctaata
    30001 gctgagacaa aatatcaatt tggtaatcta atttttgttg gtacatctca ctaatatgct
    30061 cacacatttt cgccacattg ccacttttct ccccaacctc aagcatttgt gccatctcag
    30121 caccaaacac attcgggttg atcccttgtg aaaatttgta gccttgttgt aacaaattaa
    30181 gcatagtgcc aatttcttgc tgtaaaatcg ggtcatcatt tttctcggat aaaaacgacc
    30241 tcaaggcaac atcaagcgga atatgagcat tcagcattaa ggcaatattt tggctgaaaa
    30301 agataatgcg agattgctca ataattcggc taaacagtgg cattttagcc catagagcca
    30361 ttttccactt tgcgactaac ctgctctttt ttgcaaaaaa caggtaaaaa atgaccgcta
    30421 gtagtaacgt gccaagtaat actgtgcctt gctcaatcaa gcattcggaa agggtaaata
    30481 aaatatcggt aatgaggggt aaagttttgt cttttgagcc ataaagttgg gcaaactgcg
    30541 gtacaatcaa aaccaatagc ccgatagata aagcgagcga aatcagcaac acaatcaccg
    30601 ggtaaaacag gattttcttc acttttttcg ccaatttttc caatttcgct cggctttcag
    30661 ctaaattagt cagcataatg cccagttttc cgctttgttc gcccattttg attagctgaa
    30721 tttcctgatt ggtcacaaat tcacccaatt tctccaacga ttgtgaaaaa gcatagccac
    30781 tttcaatatg ctgaatcagc tcattaagcc atagataaag gcggatattt cggcagtttt
    30841 gcaacaacat agataatgcc tgttttaacg gcatggcaga attaaccaac aatgcaagct
    30901 gtgatagcaa gctattcacc tcttcttttt tcgggtttgc tgaaagcaca aaatttcggc
    30961 taatccgaat atgctgataa ccttgtgcca gcaaaaggtt ttccaacatg tctcgattcg
    31021 ttgccaaccg cttgccccgc tgtttttgcc caaatcggct ctgggcttgc caatgaaatt
    31081 catagatttt cgtcattgcc taagcaattt ctctttttgt ggttcaaaat gaatatctga
    31141 ggcgttgtgt tgcaaacaga acttaaataa attctctaaa atttggataa tcggatcgtt
    31201 tctatccact tgttccgaat gagccaatga atacgccgta ttctcctcat aaatctcact
    31261 ctgccgacta ctctgctcag gcgaaagggc attgagtaaa aacttcaatt catcggagga
    31321 aatcaaaaca ggctcaatct gtttgtggta gataaacgca aaaatctcac aggcattgag
    31381 attttgcata tcatcaatcc ctagccaaag ctgttcttct gtttcttgca gcggaatggc
    31441 taaatatcgc aataaaatct gcttttcatt gcaatttttc tgccactgct ctgctgaaat
    31501 ctcgatgaac ctttggctgt ttaaatccgt taccgaatac tccattttga tttcctcacc
    31561 tcaaattagc cgtttggtgt aacagttgtg ctacagaaat tagctggaaa taggctcgta
    31621 tcattgccac tacaacttgc cgtccaacta atcgtattat ttgagaacga cggtgttaag
    31681 gtatagccat aaccattcag agagcctttg ccggttaccg taatcacgcc ttttgttacc
    31741 gcaattgagt ttaggtattt agtggtatta ctactggttt tattcgcctg aatcccattc
    31801 gtgccaccgg agcagttatc caacccgcca ctgttgtaaa tacagacttc aacgtctgat
    31861 ttatacgaag cagatgcttg taataactcc gataacgccg ccttttgggt gtatgagtta
    31921 taggaaggga tcgcaatagt ggcaagaatg gcaataatcg caatcacgat catcaactca
    31981 atgagggtaa atgcccgatg cagttggcga atgccaagcg gtctaaaatg tacaattttt
    32041 tgcatataaa ttccttataa attgcaccaa caaattgtgg cgaatcaagt gtgcaaattt
    32101 tttgcctgct ttgcgaccgg caaattttaa tttttcgacg cagatcgaaa aaataaaatt
    32161 tttatctgtt tatccataca gttttttgtt agaatttgcc aaatttactc aattaggaat
    32221 caagaatggc aaaagcacct aaaaccgctt atgtttgtaa cgattgcggg gctgaatatt
    32281 cccgttggat ggggcaatgt aaggaatgta aggcttggaa taccatcagt gaagtgcggt
    32341 tgatttccag caaagaagcg gcgaaatctg accgctttag cggctatgcc ggtgaaacgt
    32401 caggcaaagt acaaacgctg tctgacatca gcttgcagga agtgcctcgc tttagcagtg
    32461 gctttaacga gctggatcgt gtactcggtg gcggcgtagt gccggggtca gcaattctga
    32521 tcggcggtca cccgggggcg gggaaaagta cgttattact gcaagtaatg tgcggtttag
    32581 cccaacatct gccaacgctg tatgtcaccg gtgaagagtc gttacaacaa gttgcaatgc
    32641 gggcaaatca cttaggctta ccaacggcaa atctgaaaat gttgtctgaa acctctgtcg
    32701 agcatatttg caatattgca gatcaagaaa agcccaaaat tatggtgatt gactcgattc
    32761 aggtgatgca cttgtcggat attcagtcct cacccggcag tgtttcacaa gtgcgtgaat
    32821 gtgcatcttt ccttacccgc tacgccaaaa cccgccaagt ggcgattatt atggttgggc
    32881 acgtcactaa agatggcacg cttgccggcc cgaaagtgtt ggaacacgcc atcgatgctt
    32941 ccttgctgtt agaaggcgaa tccgattccc gtttccgtac tttgcgtagc cagaaaaatc
    33001 gttttggtgc cgtcaatgaa ttgggcgtat ttgcgatgac cgaacaaggg ctaaaagagg
    33061 ttaaaaaccc atctgcgatt tttttaagcc gtagcgaaga gcaaacgccg ggcagttcgg
    33121 tgatggtgtt atgggaagga acacgcccat tgttggttga aattcaagcc ttagtcgatc
    33181 actcaatgct ggcaaaccct cgccgtgtgg cggttggctt agatcataac cgtttatcgc
    33241 tattacttgc ggtgctacac cgacacggtg gcttgcagat gtcggatcaa gatgtgtttg
    33301 taaatgtggt cggtggggtg aaagtcacgg aaaccagtgc cgatttagcc ttaattttgg
    33361 cgttaatttc cagcttccgt aaccgtccat tgccgcaaga tttggtgatt tttggggaag
    33421 tcggcttagc aggggaaatc cgcccggtta ccagtgggca agagcggatc agcgaagccg
    33481 caaaacacgg ctttaaacga gcgataattc ctcacggcaa tgtcccgaaa aaggcaatta
    33541 aagatatgca ggtgttcacc gtgaaaaaac tcagtgatgc gttagatatt ctgtataatc
    33601 tctaaaatca agaaccgctt gtaagtctgt cttacaagcg gtctgtttta ggtaaaaatt
    33661 tacgaatgtg cggcagttgg tctattgcct ttggaaatat agtataaatt aatacacacc
    33721 accaacgcat ttaagccggt gaccggaatc gagccaatca gcaaaccata aacaacgaaa
    33781 caggcacaac ccagcgaatt caccacccgc agtttaatca catcttttaa taaaaaagaa
    33841 caggcaacta aaatcatcgc cacatagccg agtatttcaa catagtccat ttagaacctc
    33901 ctcaaataaa ataaactgcc gatattttaa aggctgtttt tttatttgta tctagctcta
    33961 aaaatagcaa aggtttgcgt tcacattttt acttttaacc gcttagttat taaacagatt
    34021 ttcgttctaa acacataaaa cggcaacgga aagggttatt ttcatcaatt tctgaccact
    34081 tttcttctac taacgcccaa ttttgttcat caaattcaaa gaacgtatcc ccttcaatgt
    34141 ctgcttgaat ctcagttaaa taaagtcttt ctgctagtgg taaggcttgc ttaaacagtt
    34201 cccccccacc aataatcatt atttcagctg tatttgcaaa atttttcgct aattcaaccg
    34261 cttgttcgag cgattgtacc gcataagccc cttccaccgt aaaacctgaa cgggaaagaa
    34321 taatattagg gcgttttggc aataacctgc cgatagattc ataggttttc cgccccataa
    34381 tcaccggttt gccgagcgtg ttttggcgaa accaggctaa atctaccggc aaatgccacg
    34441 gcatttggtt atctttgcca atcaccttgt tgattgttcg ggcaacaata atgctgattt
    34501 tcattttttc tcctgttatg caaattggtt tgattttata tcaaattaag cgtgatttta
    34561 caccctaaaa gtgatacttt tggcaacatt atttttagtt acaagtgaac agattatgtc
    34621 aaaaactatc gtgataaaat ttggcaccag cactctcaca cacggcacta aaagtttaag
    34681 ccgtccttat atgttagagc tggtaaagct caattacacc aacagcatcg tgtgattgtg
    34741 gtgacatccg gagctgccgc tgccggtcgg gactatttag gacaccctga actccctaaa
    34801 accttagcct ctaagcaaat gttagcagcg gtcgggcaaa gtcagctcat tcgagtgtgg
    34861 gaaaatttat tcgatattta taatatccat atcgggcaga tgctcttaac ccgagccgat
    34921 ctggacgatc gtgaacgttt cctcaatgcc cgtgataccc ttgatgccct gcttgcacaa
    34981 aaaatcattc cggtgattaa tgaaaacgat gccgttgcta ccgaagaatt taaagtaggc
    35041 gataacgata atttatcggc tttagttgcg attttggctc aagcagatca gctctattta
    35101 ttaaccgacc aagaaggctt attcagtgcc gatcctcgct caaaccctga tgctaaattg
    35161 ctctcggtgg tggaaaaaat cacgcctgaa atccgccaaa ttgccggtgg tagttcaacc
    35221 ggtttaggca cgggcggaat gagtaccaaa attactgctg ctgatattgc tacccgctcc
    35281 ggtgtggaaa ccatcatcgc ctccggttct cgcccaaatg tgttagttga tttagcaaac
    35341 gggcaaaaca tcggcagtaa atttttagtc caatctgacc gcttggaagg cagaaaacgc
    35401 tggttatttg ctgctccgca agtgggtgaa attgtggtag atttcggggc ggaacaagcc
    35461 ttgcttcaac ataaatcatt actgccggtg ggagtttctg ccatcaaagg cgaatttact
    35521 cgaggtgaag taatcaaaat tttcagccaa gacggcaaag ccttagcact cggcattgcc
    35581 cgttatagca gtgaggcatt gctgcgaatt aaaggtaaga aatcaaaaga aattgaatcg
    35641 ctccttggtt atgaatttgg ttctgttgca gtgcatagtg acgaaatggt ggtgtattaa
    35701 tggaattggg aattgatgtt ttagcaatct tgtttgccgt tgcgaccctt gccggcttta
    35761 tcgatgccat cgctggcggc ggtggcttac tcactattcc tgcattaatg gcagcagggc
    35821 ttcctccggc aatggcactt ggcacgaata aattacaggc gtgtggcgga tcactctctt
    35881 cctcactcta ttttattcgc aaaaaagctg ttaatttaaa acaaatttgg ttgctaattc
    35941 tatgcacttt tttaggctct gccgccggta ctatttttgt gcaaatgatc aatgtcgatt
    36001 tcttaaaaac attgttgcct ttcttaattc tgattatcgg catctatttt ttggtcagcc
    36061 ctaaaattgg cgatgaagat cgtaaacaac gcattagctt taccctattt gcctttaccg
    36121 ccgctgtcgc tctcggtttt tatgatggaa tgttcggacc ggcaaccggc tcttttatga
    36181 ctctggcatt tattttgctg ctcggcttca acctctctaa agccgtagca cacgccaaag
    36241 tgattaattt tacctccaac tttgcctcat taattttctt tacgcttggt ggtgcggtaa
    36301 tgtggaaaat cggcttaatt atgatggtcg ggcaatttat tggcggcaca ctaggtgcga
    36361 aaatggtgat gacaaaaggc aaaaaattaa tccgtccgat gctagtgact atgtctttta
    36421 tgatggtcgg caaaatgctc tacgagcaag gaatttttaa ttaacaatga ataaagcaaa
    36481 acgtattgag attttaaccc gtttacgcaa tgaaaatcca aaaccgacaa ctgaactcaa
    36541 ttacagtaat ccgtttgaat tattaattgc cgtgattcta tccgcccaag cgaccgataa
    36601 aggcgtaaat aaagccaccg ataagctctt ccctgtcgcc aacacgccac aagcgatttt
    36661 agatttaggc gtagatgggc taaaagagta catcaaaact atcggcttat ttaacagcaa
    36721 ggcagaaaat attatcaaga cctgccgaga tctgatcgaa aaacataacg gcgaagtgcc
    36781 tgaagacaga gatgccctcg aagcccttgc cggtgttggc agaaaaaccg ccaatgtggt
    36841 gatgaatacc gcattcggac accctacgat tgcagtcgat acccatattt tcagggtgtc
    36901 gaaccgcacg aacttcgctc ccggcaaaaa tgtagttcag gtagaagaaa aactgctcaa
    36961 agttgtgccg gacgaattta aagtcgatgt tcaccattgg ttgattttac acggacgcta
    37021 cacttgcatt gcccgtaagc ctcgttgtgg ttcttgtatt attgaagatt tatgtgagtt
    37081 taaagagaag acggaataac attatctggt ggtatttaat gaataactcc cctctttaga
    37141 aaagtagggg ctgggggaga tttgatacta tttcaacatt tcgtatgagc gtatagaaat
    37201 tctgccaaat cttccctatc ccctctttgc taaagagggg aactagatcg agcgtaaaat
    37261 ataacaagcg gtcatttttt tgagattttt tgcaaatgga tatttattta gtcggtggag
    37321 cagtgcggga tcaacttctt gggctgccag tgaaagatag agatttttta gtggttggcg
    37381 caactgccga acaactttta gcccaaggat ttcaacaagt tggggcagat tttccggtgt
    37441 ttttacaccc tgaaaccaaa gaagaatatg cccttgctcg ccaagaacgc aagaacggca
    37501 atggttacaa cggctttatt tgcgatttct ccccaaacgt cacgcttgag caagatttga
    37561 tccgccgaga tttaaccatt aacgccatcg cccaagatga aaacagcaca ctttttgatc
    37621 cttatggcgg tgtgcaagat ttagaaaatc ggctactccg ccacgtttct cctgcattca
    37681 gcgaagatcc gctacgggtg ctacgggttg cccgctttgc cgcccgtttt cattcatttg
    37741 gttttaccat tgctcctgaa accctgaaat taatgcgaga gatggtacaa agcggtgaat
    37801 tgaaacattt aaccgctgaa cgtgtgtggt tggaaacaca gaaagccttt gaaaccgata
    37861 atccgcacgt ttattttgac attttacgct taattggggc attaaaagtg ctattccctg
    37921 aactggatcg tctgtttggc gtaccacaac cacctcaaca ccaccctgaa attgactccg
    37981 gcaaacacac tctaatggtg ctacaacagg caaaaagact tgcaaaaaaa gcggaaaatc
    38041 cgaccgcttt actctttgca gccctttgcc acgacttagg caaaggctta acgcctgctg
    38101 atattttgcc tcatcactac ggacacgaag tcaaaggcat tcaaccaacc cgagagttag
    38161 ccaatcggtt aaaaatccca actgagatta aagattttgc ccttttagtg accgaatacc
    38221 acacgcattg ccataaaatg gcggaacttc gccctgaaac ggtaataaaa ctgtttaaca
    38281 gcctagatgt gtggcgtaaa ccaaaccgct tttttgattt cctgttcgcc tgtgaagcgg
    38341 atgctagagg gcgattaggt tttgaacagc ggaattaccc ccaacctgaa ttggcgaaaa
    38401 aatattacca agccgcaatc gaaattgatg tgcagcaagt tattcaagac ggttttgaaa
    38461 aacaagcaat tcgggaagag ctgaataaac gcagagtgta tgcggtaaga agcatcaagc
    38521 tagatagcga cctaataaac cattaggtta tccgccactt tctcaaaatc aattcccaaa
    38581 gttcaagttg gttaaatgta tggattcttt aaaaggtggc gttattttag tgaatttata
    38641 atgttcctat gctcttgatt gttgtagcca caatttcagg gtaaagatca gcaataaccc
    38701 aatgccgaaa ttggcaatag tgaagccggc taacccaata aaagataaag gttcaaattg
    38761 ataaaattca atcgcacctc ttgccattgc agctaaacca aatgaaaatg cccagaaacc
    38821 catcgagaag ccattttcag ctatccattt gaggtttcgt aaaataaaac agaattgtaa
    38881 gaatccatat ccccataaca tttttgccaa caagtctaat tctccaccca tagaaaaata
    38941 ggcagaagcg cccacaaatg ccggagcaag aacaattcct aaagatggtc tagcaatcgg
    39001 aaaaagcgga ctaagccgaa ttctttgtaa catagtgggt tcatatacta accaggaaat
    39061 tatgccaatg cctaaaaata aagatgcaat ttcactgtag ccaattaagc ttaatgaact
    39121 tgccatagtg aagctccctg caattggcgg taggtaatgt accgggtgta gcgtttccgg
    39181 caaaaattgt gtgcctttcc ataatccacc aataataaaa accaagaaaa ttaaatgcac
    39241 aatgacgcct aaaaaaatca atagttcgcc taaataaaaa tcccatacat agagcacatc
    39301 accgcttatc atggttgtca ttgtcactaa ggaaaataaa caacattgca ctgggtgcat
    39361 aaattctgct ttaatctgct caaaacaaaa aaacacacga tacacataac agcctaataa
    39421 ggctacccaa agtatgactg acgttccagc aatgatatta ccaattgttg tagcataagg
    39481 agtgataggc gttgcattta accaggcaag gcctaacgcc cccagcccta atggcattgc
    39541 aaaaaagcct gtagttattg gaaaaggttt agattcactc ataaatttat cctcatattg
    39601 aaataatgca taactgtatt cggctttata acagtcatat gtaaaaaatt ttatttttct
    39661 aaccgcttgt aataagaaaa acctaaagca cttgctttag gttcctacta ctattcatac
    39721 tcttctagcc agatagttaa aatcgcttca agaatatgtt catttgatgc ttccggatca
    39781 tcatcgaaat cttccatttc acaaatccat ttatgcatat ctgtaaaacg aaccgtggtc
    39841 gggtctaaat ccgggttcat atcatacaga ttttcagcga tcattcgggt atctgtccat
    39901 ttcattagtg cgctgcctca tttgcgtgat ttaaattata ttttggaatt tcaacaacca
    39961 aatcttcttc accaacaata cattggcaac ttaaacggct gtctatttcc aatccccaag
    40021 ctttatccag catatcttct tcttgatctg aagtatcatt taacgaatca aaaccctcac
    40081 gaattaccac gtggcaagtg gtacaagcac aagaacagtc gcaagcgtga tgaatttcaa
    40141 cgcctgcttc gtgtgctact tctaataaat tatctccggc tttagcttca acaaccatac
    40201 cttccgggca aaattcttcg tgtggaagaa aaacaatttt tggcatttta aagtttcctt
    40261 attactaatc ccattcaata ttatccagat gttcgccgat gaagtttaaa atctcttcct
    40321 cattttcaac cagataaaca ataatcttct ttaattccct tgctttctct ttacttttct
    40381 cggaaagcgg ataggtaaca taataacatc ctacgtccgg atcttcttct aataactcta
    40441 ataggcttgg acgataaata ctaagataag cccgtaaaaa tgcagcccag ttataaccgt
    40501 tcatataggc aacatcatat tcagtattaa tccgctcgcc caactcaaaa atgcggttat
    40561 cctctaaatc gaattcaacc gcaacggttc tgaattgttc tgaaatctcg gtttttacat
    40621 agttagacat actgcctcct tatgctaaat cgtccatttt tttaccggct aatgcttttt
    40681 gaattgataa attcatacgc cgtgctgcaa attcttgcgt agctaaatcc acctctttga
    40741 taccttgctg aattgcaaag cgatcagtac cttcttttaa ttcgattaaa cgggctaatt
    40801 gtgcttcaat cgcttggaac tcctccacac tcaacactgc agagccatct tctttcaatg
    40861 ccgttaccac gctgtcaatc actcggtcgg cttctactcg ctgttcaact aactggcggg
    40921 cttccatatc ttctttggca tttgccattg aggatttaat catctgcgta acttcttcat
    40981 cggttaagcc ataagacggt ttgatttgaa ttgaagcctg cactttggtc gatttttcca
    41041 ttgcagtcac gtttaataaa ccgtctgcat caacttggta agttacccga acctgtgcaa
    41101 cccctgctgc cattgccgga atgccacgca aagtgatttt accgagagag cgacaatctt
    41161 cgactaattc acgctcacct tgtaaaacgt ggattgtcat cgccgtttga ccgtctttac
    41221 cggtagtaaa ctcttgagct cgagctactg ggatagtagt gttgcgtgga ataattttct
    41281 ccaccaaacc acccatcgtt tcaataccaa gcgaaagtgg cacaacatca agcaacaaca
    41341 tatcggaatc cggtttgttt ccaactaaaa tatccgcctg cactgccgca ccaatggcaa
    41401 caactttatc cgggtcgata gaggttaatg gcgttttacc gaaaaactcg ccgacttgtt
    41461 cacgcacaaa cggtacacgg gtcgaaccgc ctaccattac cacttcacga acctcttctg
    41521 cttcaactcc ggcatctttc aaggctcggc gacaagtcat cagcgagcgt tttacaagcg
    41581 gttgaattag ctcattaaat tgcgaacggc ttactacgcc tgtccaattt gcaaatttca
    41641 cttcaaattc aaccgcttgc gaaagtgcga ctttggtttg agttgccaaa gttaataatt
    41701 cacgctgctc gcttgccgtt tgaggtttgt aatttgcctg ttcagcaatc cattccgcta
    41761 ataggtggtc gaaatcatcg ccaccgagtg cggtatcacc tgcggttgct aatacttcaa
    41821 acacaccttt gcgtaaacgc aaaatagata aatcgaaagt accgccacct aaatcgtaaa
    41881 cggcaatcac accttcttgt ccgctttcta aaccataagc aatcgctgct gccgttggtt
    41941 cattgagcaa acgtaataca tttaaacccg caagacgtgc ggcatctttg gtactttggc
    42001 gttgtgcgtc atcaaaataa gccggtacgg taattaccac gcctgaaagc tcaccggcta
    42061 aacgcttttc ggcaaattgt gccaagtggc ttaaaatatc cgccgaaact tcaatcgggc
    42121 ttttattacc ttgcgctgtt tggattaaag gtaaaccgtt ttcgctcgcg acaaattgat
    42181 aaggtaaatt ttgataacgt tgctgaacat ctgccagaga acgcccaatg agacgtttag
    42241 ctgaaatgac ggtatttttc gggtcaagtg cagctttggc aaaggcttca atcccaactg
    42301 ttttttctgt ttccgcataa tgcactactg acggcactaa tgctctttct tgttcatcaa
    42361 gcaatacctg agtctgcccg ctacgcacag ttgccactaa tgaattggtt gtacctagat
    42421 caattcctac cgctaattta tgttgatgtg gtgcagcggt ttgaccgggt tcagaaattt
    42481 gtaataatgc catttattta tctctttatc tttctaactt agttctctat tgtattaaat
    42541 ggagtgaaat atattgttta agagaagctg gag
//
LOCUS       DANLUA010000004        38866 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLUA010000004 DANLUA010000000
VERSION     DANLUA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 38866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38866
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(408..2525)
                     /gene="spoT"
                     /locus_tag="PXB44_000199"
     CDS             complement(408..2525)
                     /gene="spoT"
                     /locus_tag="PXB44_000199"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL5280724.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(2635..2916)
                     /gene="rpoZ"
                     /locus_tag="PXB44_000200"
     CDS             complement(2635..2916)
                     /gene="rpoZ"
                     /locus_tag="PXB44_000200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL5280725.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(3019..3516)
                     /locus_tag="PXB44_000201"
     CDS             complement(3019..3516)
                     /locus_tag="PXB44_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL5280726.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(3541..4563)
                     /locus_tag="PXB44_000202"
     CDS             complement(3541..4563)
                     /locus_tag="PXB44_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5280727.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(4603..6033)
                     /locus_tag="PXB44_000203"
     CDS             complement(4603..6033)
                     /locus_tag="PXB44_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL5280728.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSMLTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(6138..7061)
                     /locus_tag="PXB44_000204"
     CDS             complement(6138..7061)
                     /locus_tag="PXB44_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL5280729.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(7178..8605)
                     /gene="miaB"
                     /locus_tag="PXB44_000205"
     CDS             complement(7178..8605)
                     /gene="miaB"
                     /locus_tag="PXB44_000205"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL5280730.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            complement(8822..9322)
                     /gene="crr"
                     /locus_tag="PXB44_000206"
     CDS             complement(8822..9322)
                     /gene="crr"
                     /locus_tag="PXB44_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL5280731.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            complement(9407..11128)
                     /gene="ptsI"
                     /locus_tag="PXB44_000207"
     CDS             complement(9407..11128)
                     /gene="ptsI"
                     /locus_tag="PXB44_000207"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL5280732.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            complement(11250..11507)
                     /gene="ptsH"
                     /locus_tag="PXB44_000208"
     CDS             complement(11250..11507)
                     /gene="ptsH"
                     /locus_tag="PXB44_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL5280733.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            11768..13342
                     /gene="thiP"
                     /locus_tag="PXB44_000209"
     CDS             11768..13342
                     /gene="thiP"
                     /locus_tag="PXB44_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL5280734.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            13339..13989
                     /gene="thiQ"
                     /locus_tag="PXB44_000210"
     CDS             13339..13989
                     /gene="thiQ"
                     /locus_tag="PXB44_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL5280735.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            14296..15710
                     /locus_tag="PXB44_000211"
                     /pseudo
     CDS             14296..15710
                     /locus_tag="PXB44_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(15826..16629)
                     /gene="map"
                     /locus_tag="PXB44_000212"
     CDS             complement(15826..16629)
                     /gene="map"
                     /locus_tag="PXB44_000212"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL5280736.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(16715..17380)
                     /locus_tag="PXB44_000213"
     CDS             complement(16715..17380)
                     /locus_tag="PXB44_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL5280737.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            17573..18262
                     /locus_tag="PXB44_000214"
     CDS             17573..18262
                     /locus_tag="PXB44_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL5280738.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            complement(18330..19121)
                     /locus_tag="PXB44_000215"
     CDS             complement(18330..19121)
                     /locus_tag="PXB44_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5280739.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            19245..20249
                     /gene="fbp"
                     /locus_tag="PXB44_000216"
     CDS             19245..20249
                     /gene="fbp"
                     /locus_tag="PXB44_000216"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL5280740.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            20433..24944
                     /locus_tag="PXB44_000217"
     CDS             20433..24944
                     /locus_tag="PXB44_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5280741.1"
                     /translation="MINKNFPLSYLASFVAFAVSLSATASVFRDDIDLQYYRDFAENK
                     GKFTVGASNIEIKDKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQ
                     YNTVEFGAPGTNADAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTD
                     LGNNASAYQDSSRFTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSD
                     YWLDFRGNALNNSPYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYN
                     RAGIIRQDYHDKQFAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSI
                     KDNSASPLGYGGTPQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVR
                     PENDQSWVGAGIVVAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGT
                     VILTQQAGENGKKSAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIA
                     FNRIQNSDDGARIVNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHAN
                     NRTDYFTLKGNPNQYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIG
                     ETDNTQHNGRLNVVYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALK
                     GDPKQPVPRGGLSSEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNG
                     DLTVKGGKVLLSGRPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNV
                     SNLQANLSANDHAQLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQIN
                     GDVSLAEQSQLHIGKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTIN
                     LNEKFVTGEIPTRFNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALR
                     NSGKEAEVVSPLSLLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLK
                     DAKDRNQSIATAKAELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQL
                     SQANSELTRLQQYADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAA
                     QIANVEQAVETAQNIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAH
                     ADTDSSNIQQSDWVSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEH
                     QKTEHQSDLYRPYEQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLL
                     MASLYGKWHSENGTFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQ
                     VQPAVGTRYYRFSAQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAH
                     YVDASSKRLSVAVNDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHF
                     AGFKLGYSW"
     gene            complement(24999..26108)
                     /gene="mobA"
                     /locus_tag="PXB44_000218"
     CDS             complement(24999..26108)
                     /gene="mobA"
                     /locus_tag="PXB44_000218"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5280742.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            26200..26469
                     /locus_tag="PXB44_000219"
     CDS             26200..26469
                     /locus_tag="PXB44_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL5280743.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            26513..27151
                     /locus_tag="PXB44_000220"
     CDS             26513..27151
                     /locus_tag="PXB44_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5280744.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            27171..27623
                     /locus_tag="PXB44_000221"
     CDS             27171..27623
                     /locus_tag="PXB44_000221"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL5280745.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            27607..28269
                     /locus_tag="PXB44_000222"
     CDS             27607..28269
                     /locus_tag="PXB44_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5280746.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            28271..28564
                     /locus_tag="PXB44_000223"
     CDS             28271..28564
                     /locus_tag="PXB44_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5280747.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(28633..29967)
                     /gene="srmB"
                     /locus_tag="PXB44_000224"
     CDS             complement(28633..29967)
                     /gene="srmB"
                     /locus_tag="PXB44_000224"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL5280748.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            30127..30390
                     /locus_tag="PXB44_000225"
     CDS             30127..30390
                     /locus_tag="PXB44_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL5280749.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            complement(30438..30536)
                     /locus_tag="PXB44_000226"
     CDS             complement(30438..30536)
                     /locus_tag="PXB44_000226"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL5280750.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(30536..32044)
                     /locus_tag="PXB44_000227"
     CDS             complement(30536..32044)
                     /locus_tag="PXB44_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5280751.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            complement(32373..33662)
                     /locus_tag="PXB44_000228"
     CDS             complement(32373..33662)
                     /locus_tag="PXB44_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL5280752.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDSA"
     gene            complement(33786..34583)
                     /gene="ccsA"
                     /locus_tag="PXB44_000229"
     CDS             complement(33786..34583)
                     /gene="ccsA"
                     /locus_tag="PXB44_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL5280753.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            34799..35323
                     /gene="yceD"
                     /locus_tag="PXB44_000230"
     CDS             34799..35323
                     /gene="yceD"
                     /locus_tag="PXB44_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL5280754.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            35340..35510
                     /gene="rpmF"
                     /locus_tag="PXB44_000231"
     CDS             35340..35510
                     /gene="rpmF"
                     /locus_tag="PXB44_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL5280755.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            35529..36551
                     /gene="plsX"
                     /locus_tag="PXB44_000232"
     CDS             35529..36551
                     /gene="plsX"
                     /locus_tag="PXB44_000232"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL5280756.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(36648..37931)
                     /gene="hisD"
                     /locus_tag="PXB44_000233"
     CDS             complement(36648..37931)
                     /gene="hisD"
                     /locus_tag="PXB44_000233"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL5280757.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(37943..38842)
                     /gene="hisG"
                     /locus_tag="PXB44_000234"
     CDS             complement(37943..38842)
                     /gene="hisG"
                     /locus_tag="PXB44_000234"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL5280758.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
BASE COUNT        11502 a         8630 c         7498 g        11236 t
ORIGIN      
        1 gtacattttc gagaaaattt tcgctaagcc caccatcttg attaggcttt ctagatatat
       61 gccaaaatcc agataaacga taatacccta cctgagataa ttttttctct gcatagcttt
      121 tatcatttat gagcattcct ctagatatga gtagttctac taattcgtga tactctttat
      181 gtggttttgt aggcataaaa ttgaaggcat tttaagaaag gcaaggacgt agcttgacac
      241 tctaagtcga ttacatttag taaacctcag ggacaggggc tacgccacga ttgcaaggga
      301 ttatacattt tagctgatta aagtcaaaag gtttcatcaa ttaataatag caatactgta
      361 cttttaagga ctataattgc aaaaaactga taaaatttta ccgcttgtta ctgcttacgg
      421 gccgctttta caacaccttt tacacttaat agcttacgga gaatcagctc agaatgctct
      481 ttattttcca cagaaatttg tactctcact tgatagatat ttccttctct tgcctcgcta
      541 ttaatgctgc caatgttgct atttaatttg gcaagcgttg atgtgatccc cgcgagtacc
      601 cctggttcat tgataatatc cagtacgatt tccgtttcaa attgaatcgc tttttctttg
      661 gcaatttccc attccacagg taaataatta ctcggctctg ttgcgtaatc tttcacatta
      721 ccacaacaag cattgtggat tgccaatcct ttacctgaac tgacgtgagc aataatttca
      781 tcacccggta taggtaagca acagtttgca aaactgataa ggcggttctc aatcccttca
      841 attttaagcg gttgattatt ttctggatta ccatcagtat caattaatag tggctcccct
      901 aataagcgtt gagcgataac attactcatt tggttaccta gaccaatttc agccagtagc
      961 tcatcaaagc ttgataaacg tagctcgtct aatacggatt gaatagtttc tgaacacaat
     1021 tcatcaagtg agcgtaaatt cattgcccga actagctgtc tacgcccaag tgcaatcgcc
     1081 tcatcgtgct gctgattttt aagcgattga cgaattttag ccctcgcctt accacttact
     1141 acaaaattta accacgatgc atttgggcgc ttaccttgct cggtaataat ttccaccgtc
     1201 tgtcctgttc gtagcggttc tgaaagtgga tacggattgc gatcaaccat cgcacctaca
     1261 caacgatccc caatgcctga atgcaccgca taggcaaagt caatagcggt agcattcgcc
     1321 ggcaattcaa caatacgccc tttcggggta aagacgaaaa tatcgtcaga gaacagatcc
     1381 gatttaacgc tatcaataaa ttctgctgta ttgccggcac tgttttgcaa ttcaacaata
     1441 ctttgtagcc attgctgggc tttgagttga acggtcgtgt tatggtgcga ctcgccctct
     1501 ttgtaacgcc agtgagctgt tacccccata tccgccattt tgtccatatc ttcggtgcga
     1561 atctgtacct ccaccggtac accgtgcggg ccaatcatcg aagtgtgtaa agattgatag
     1621 ccgttcggtt ttggcacggc aatataatct ttcacccgtt tcggacgagg cttatacagt
     1681 gcgtgcatct gccctaatac acggtaacaa ttatctacat tatcaaccac tactcggaaa
     1741 gcataaatat ctaaaatcga atgaaaatgt tgatctttct ggcgcatttt ttggtaaatg
     1801 gaatagagat gtttttcacg cccatacaca cgggcttcaa tttgcacctc ttctaaccga
     1861 ccacgaatat catcggcaat gcgttgaatt agctctttac gatgtcctct ggcactggca
     1921 atagccattt tcagtacccg ataacgttgc gggtgcattg cctcgaagcc taaatcttcc
     1981 aactcgtttt taatatgctc tattcccaag cgatgtgcca acgggctata aatctctaag
     2041 gtttctttcg ctatacggcg gcgtttatcc ggacgcaaag caccaagcgt acgcatatta
     2101 tgggttcggt cggcaagttt aattaacaca acccgaatgt ctttggtcat tgccaaaatc
     2161 attttacgga agtttgccac ttctgcctct tggcgggtgc ggaattttaa tttatccagc
     2221 ttagaaacgc cttgtacaat atctgctacg ctctgtccaa attccgccgc cagttcttct
     2281 tcggtgtaag gtgtatcttc aatcacatcg tgcaacaaag ccgcaatcac cgcttcttga
     2341 tcgagcttca tttccgcaat aatcgacgcc actgctaccg ggtgagtaat atagggctca
     2401 ccgctagagc gtgtttgtcc ctcgtgagca tcacgggcta tcacataagc acgttttaca
     2461 cgctcaatct gctcgggtgg caaatagcct tgaataattc gatctaaagg ttcaaaaaga
     2521 tacacgttat acctcaacaa cagaaataaa aatggacaac gaatttgcaa attttgagga
     2581 aaatcctacc gcttgcagac tcaattgtcc attctctatt tacgaaaaat aaaattacgc
     2641 ttcgttgctc atttcagtta aaaatgaaat cgcctcttgc tccgccgctt cttgcttaat
     2701 agagtcgtga ttttccagct gatccataat gtcagttgta attaaacctt cttcaatttc
     2761 acgtaatgca atcacggtcg gcttatcgtt ttcttcaggc acaagtggct cacgggtgtg
     2821 aagttgtaat tgtcttgcac gacgtgcagc aattaagatt aaatcaaaac ggttgccaat
     2881 tttctccgct gcttcttgta cggttacacg agccataaat ttactccaaa gatttattcg
     2941 gttttattca taaaaagatt gcgaattata cagaaattcc gccaattccg caatgaaagc
     3001 gggctaaatt cacaaaattt atttggtatt cagtaaaatc gccaataatt cagcctcaac
     3061 ctctttgccg aattttccca ttctaaaatc ggcttttagt tgctcgaatt gctcagggtt
     3121 cgctgccatt ttatctttta aaaattgcgg aaattcagat aacactaatt ctgttttgcc
     3181 attacgaata tcaaaaatag ttggaatttt ttgcgcagaa atgaccgctt gttgctgttc
     3241 atcgctgtca aaatgtttgc gttcctgatt atctttgcga gccacatatt tcaccttaat
     3301 gttaggattg agctttgcca ttcgctggaa ataagccaca atttccacac aatcggggca
     3361 atatggctct gccattacca gccagtcccc ttgcacgcca acagcttgca gtttttcttc
     3421 cgtatctgtg gaaagcttga cctccacata cacttctaat tgattttcac gatcttcatc
     3481 gctgttaaat tctaaatatt cgtaaaaatg tgccataatt ttgtcttttc ctatctcatc
     3541 ttaaatggat ttacgccata aaatttcaca atcaacttta acgtcatcta ttttctgttg
     3601 cgctaacttt tgctgaatta attcaaaggt acgttcggca atttgttggt ggtcttgctt
     3661 aattgaatga atatgaaaag gtagtgcatt cagtaaatgg tgatcatcaa aggttgctaa
     3721 atgcagttct tgagtcaata atttatccat ttgttggtgt tcagtcagat agcgcattac
     3781 tccctcaagt agggtaaaag aggctgtgaa taccgcttct gggagcctgc caagtttttc
     3841 aactatctga gcgaacattt gatagcccga ttccggctga tagtcttgat gcagaaccca
     3901 attttcatca agtgccaaat gtgactgctc caaaccttca cggaagcctt ttaaacgatt
     3961 ttgacttggc gagagtgaaa gttgtccacc taagtaaaaa aactctttta agtgataagc
     4021 ccgtacaata ttttccacca acatactgat actttgtgta tcattactgg tgatagaagg
     4081 gatattcaac ccagaaatat agcggtctag ctgtaacatt ggggtatatt gtgccgcatt
     4141 ttgataataa gtcggctcat tatgtgtcgg agcagtaatc agtaaatcta cctgtctgtc
     4201 taataaacgc tctatcgctt tcttctcacc ttcaaaggta tcatccgagc aagcgatcac
     4261 tagctgtaac ccgttttcac ggcataattt ctctaaggtt cttgaggtca gagcaaagcc
     4321 ataattcgtt aaatcgggaa tgactaaacc gatcacattc gtgcgttgag cctgtaatga
     4381 ttttgcatat acattagcac gataaccgtg ttttttagcg atctctttca ctttttggca
     4441 ggtttcggat ttgattctaa aatcattgcc ccgcccattt aaaatcatac tgactgttgt
     4501 tttagatacg ccgctgagtt ccgcaatatc actgagcgtt aggcgttttt tagtcacact
     4561 tatctccttt tagcataaaa atgggcatta ctgcccattg ctttattcta cgctaattgg
     4621 caatactttt gctactttca cttgaatcgg acgagagctt tttacgacat tttcacgctt
     4681 agcgataaaa aagcgtgaag tcagcacttt ttccccgccg tttacgaaaa tttccaccac
     4741 agaacggtca aagaaaatct cgattgtctc taattgttca attttgcaat gacgaaccga
     4801 gccaaatttt tccatcaatt ctgtttgttc ggtttggcta cgatctaagc tcaacacccc
     4861 attttcaaaa cgtaagctga gcttttgccc ctgttcattg ctgaaaaaat ccaacacaag
     4921 cggttgattt tccacagaaa tttgcaagta ggcagtatcc aaattctcga ttgcaaaatt
     4981 ttctgacatt tcgaccgctt gtaattcgcc tacattcacg attgggtttt gtatcacttt
     5041 gccgttttgc agggaaagtt tacgaggcaa tgtcagcatt gaatgccact gatatttatc
     5101 agttgggtaa gtgagatcag gcaagccgac ccagccaaac aaaatgcgat cggaattttc
     5161 caccgtttgc ggtgcgtaaa agtcaaagcc gtagtccagc tcttcaatat gctctgcccg
     5221 taatctgttg ccgtttagct cgccaagtgc gtaagtggcg tggtagttat tttggaattg
     5281 atggctttca cgcagtttgc cttgtggcga ccacacaaac agatctttac caccaagtga
     5341 gaacagatcc ggacactccc acataaacac attgctgtta tcaaaattgt ggacttccag
     5401 ttctgcgatt aaacggggtg tggcatttaa atcgtccatt tcataaatta acgccgtgcc
     5461 ggtcaagttt tcacgctgag cccccaacac aaaacgaatt ttgccctctg cggtaaggta
     5521 aggtttcggg tcacgcacgt gttcggtgta gcctgccggt gcttggtcga taatgcaacg
     5581 cttttccaat aatttgcccg atttatcaaa aatcgcgata ttttggtgag gaatgcgggc
     5641 attatctgat gggcggcgtg tattgccggt gtaaaaagcg acaatcttat cttgccataa
     5701 aatcgcccca cctgaataac agccgtggga ttcaaacagc tcatcgggaa tcagcaaatc
     5761 ccctttactg aacgtttgaa aatcggtagt gatgaaatgc tgccaatgtt tcattccatg
     5821 taatgcccca taaggaaacc attgggcgaa aatatggtat ttttcaccat cgaaaatcaa
     5881 gccgtttggg tcattaaata agcctgtttc gggagcgaga tgataggttg ggtaaaaatc
     5941 gggatcagat aaaacggttt ggcgaagagc gtttaattcg ccctcgcttt gtgcgtgcag
     6001 gcttttgtat ttgccgttgt tgaaaatgtg catagagtgt tcctgtcatt tcagaaatta
     6061 cgatggtgca agcgtccacg cttgtaccct tttgtaaaaa atgaggcaca agcgtggaca
     6121 cttgcgccat cattacatta ttccgccaaa aatgcgtcta actccgcttt attcggtaat
     6181 gccgacatcg ccccttttgc tgtggtggct aacgcaccgg aagcatttgc tttgcggatc
     6241 acttcaacta acacagtttc atcgtgccaa ttcggcgttt gagcaagacc ggctaataat
     6301 ccgctgacaa aggcatctcc ggcaccggtg gtatccacag gtttcaaggc tttgccggca
     6361 acgacttggc tttttccgtt gaaatggtag attgcaccgt ctttgccaag ggtgataata
     6421 atcaatttct ccggatactg agcggtaata gcttgtgtgg cttgttccaa agtttctgtc
     6481 tcggttaata aggtcagctc ttcttcggaa aatttgagaa tatccgccat cgccaccaca
     6541 gaatttacca ctgttttcat ttcatcaagg ctcttccaga gcgattcacg caagtttgga
     6601 tcaaacgaaa catagccacc cgcctttttc actcggcgaa tcccttcaat ggtagtggaa
     6661 cgggatgggt cgttaatcaa ggcaatagaa cagcagtgta agaaatcacc ttggttaaat
     6721 tcaggtaaat cgccgatttc taaaaattgg tcggcactcg gatttaccat aaaagtaaaa
     6781 ctacgctcac cgttatctaa gccaacaatc acggtggaag tgcggtgctg agcgtctaaa
     6841 atcatctgtt tggtgcaaac attttctgcc tgtaacactt ctcgcataaa tttacccaga
     6901 gggtcgttac ccacacgccc gataaatcct gcctcaaccc ctaaacgaga aacgcctact
     6961 gcaacattcg ccggagcacc accggcacat tttaaatagt gatgttcgcc gtcgggaatt
     7021 aaatccacca ccgcatcgcc tgttacccaa atcttactca tctcatacac ctttaattag
     7081 tcacaaaaca ccaagctaaa acgatttagc atttattcga ttatatacta aaagaccgct
     7141 tgtacatacc tacaagcggt cattttttct ttaattttta cagattaacc acgtatttac
     7201 ctacacctaa atcatcttct ttacgagtgc gggcaatcac gctttctgcc gattccacca
     7261 cacgtaatcc catttggtct tcggtacgaa tcacttcgcc acggagtgag ttggtataaa
     7321 catcggtgat tttcacatcg acaaatttgc caatcatatc cggtgtgcca acaaagttta
     7381 ctacacggtt agtttcggtt ctgccggtta attccatcaa gtctttctta gacgggcctt
     7441 caaccaaaat gcgttgttct gtaccgagca ttgcacggct aaattgcatt gcttggtggt
     7501 tgatgcgttg ttgtaaacgg gataatcgct cttttttctc atcatcggag acattatcag
     7561 gcagatctgc cgctggtgta cccggtcgag ccgagtagat aaagctaaag ctcatatcaa
     7621 agttcacttg ctcgatcact ttcattgttt gctcgaaatc ctctgcggtt tcgcccggga
     7681 agcctacaat aaagtccgaa ctaatttgaa tctcaggtcg aacagcacgc aatttacgga
     7741 taatcgcttt gtattccaac gctgtgtggt tacgcttcat cattgttaat acacggtctg
     7801 aaccactttg aatcggtaaa tgtaagaaac tgaccaattc aggtgtatca cgataaactt
     7861 cgataatatc atcggtaaat tcaatcgggt ggctggtggt gtaacgtaag cggtcaatac
     7921 cgtcaatcgc tgccactaag cgtaataatt cggcaaaggt acaaatgccg ccatcaaaag
     7981 tttcaccacg gtaagcattt acgttttgac cgagtaaatt aacctcacgc acaccttgtg
     8041 ccgcaagttg ggcgatttca aataatacgt catccaccgg gcgggaaacc tcttcgccac
     8101 gagtgtaagg cacaacgcag aacgaacaat atttattgca gccttccata attgaaacga
     8161 atgctgtagg gccttcggct tttggctccg gcaagcggtc aaatttttca atttccggga
     8221 aagaaatatc gacaatcgag cttttaccgc cacggatttg gttgatcatt tcaggcaaac
     8281 ggtgcaaagt ttgtggcccg aaaacaatat ctacataagg ggcacgatca cggatatgct
     8341 ccccttcttg tgaagccaca caaccgccaa caccgataat caggttcggt ttatcttttt
     8401 tccagttttt ccaacgcccg agttgcgaga atactttttc ttgtgctttt tcacggatag
     8461 aacaagtgtt tagcagcaac acgtctgcat cttcagggtt ttcagtcagt tcaaaaccgt
     8521 gagtagagtt taggagatcc gccatttttg atgagtcgta ctcattcatt tggcagcccc
     8581 aagtggtaat atgtaattta gccataacgt ttaaaattaa taaataattc aataacttag
     8641 aaaagaaaag cgtaaagcaa tttattattt ttgacttaaa ataaaccctt tacgcaaaat
     8701 ttgtggcgtt attttacctt gttgcctatt ttttatctat aaataatcgc catttatccg
     8761 attttaccta aaacaaaaaa gccacgatct tccatcgtgg cttccagcta agtaggaggt
     8821 attatttggt taaggttaaa accacgcttt caccggcaac aacttggcca tttaatttag
     8881 ataagttgct gatttcatcc atatttgaaa tcacgattgg tgttaatact gatttggctt
     8941 tgccttcaag tagagctaaa tcaaatttaa tgatcggctc gcccactttt acggtttgac
     9001 cttcctgtgc aaggcgagta aagccttcgc cttttaattc aacagtatca ataccgaagt
     9061 gaacaaataa ttccacgcct tcttcagatt caatcgaaaa agcgtggttg gtttcaaaaa
     9121 ttttgccgat agtcccattt actggagcaa cgatagtgtc tccactcggg cgaattgcca
     9181 caccgtcacc tacaattttt tcagagaaaa caacatcagg cacatcttca atatttacga
     9241 tttcacctgt taatggcgca tagattttga tttcttgact ctctttttgt tttgagccga
     9301 ataatttatc aaataaaccc atcttaaaat ctcccttaga tattagatac aaaattctcg
     9361 tacattttat acgagaattt tgtatttgta tgctaaataa ttaaaattag tttaatttct
     9421 gataaaactc atctactaag cgttcaatat ctgcagcagt tggctgagca agcgcctcat
     9481 cagctaatgc tttagcatcg gcaaaattaa ttgagcgaat gagtttttta acgtgtggca
     9541 cagagatacc gctcatacta aattcatcta atcccatacc gagcaacaac gcagttgcac
     9601 gcacgtcacc ggctaattca ccacacatac ctgtccattt gccttcagcg tgtgaagcat
     9661 caataacttg cttgattaag tttaagactg atgggcttaa tgggttatat aaatgagcaa
     9721 taatttcatt accacgatct accgcaagtg tatattgggt taagtcatta gtaccaatac
     9781 taaagaagtc ggcttctttt gcaagatgac gtgcatttac cgctgctgat ggggtttcca
     9841 ccataatacc tagttttaag ttctcatcaa aagcgtgacc ttctgcacgt aattgatctt
     9901 tcaattccgc aacgatggat tttaataaac ggatttcttc caccgaaata atcatcggga
     9961 acattaccgc taaattaccg aacgcagaag cacgtaatac cgcacgtaac tgagtatcta
    10021 aaatttcacg gcgggtgaaa ccgatacgca ccgcgcgcca acctaagaat gggttcattt
    10081 ctttcggtaa atcaaggtat ggtaactctt tatcacctcc gatatccatc gtacgcagaa
    10141 tgaccatttg accattcatt gcttcaacaa tctctttgta ggcttgaaat tgctcttctt
    10201 ctcccggtaa ttgagtacga tccataaaca aaaactcagt acggtataaa ccaaccgctt
    10261 caccaccgtt gcgtaataca ccttccacat cacggatagt accgatattc cctgccacat
    10321 caactttatg gccatctaat gtttgagcag gcaactcttt taatttggct aattcttctt
    10381 tttccgcatt taatttctct tgtaatgctt tgaaattagc gattttttct tccgatggat
    10441 ttaaatgtac ttcattgttc actgcatcta aaatcagaaa atcaccagtt ttaactaggg
    10501 aagtaacatt gttagtacca acaatggcag gaagctctag ggaacgagcc ataatagaag
    10561 tgtgagaagt acgaccaccg acatcggtaa taaagcctaa tactttatct aaatttaatt
    10621 gagctgtttc ggaaggggtt aagtcataag cgactaaaat aacttcatct tgaatatcac
    10681 caagatctac gatattcata tttaaaatat tgcgtaccaa gcggttacca atatcacgaa
    10741 tatcacctgc acgttctttt aaatattcat cgtcaatttc cgcaagcatt gaagcctgca
    10801 tctcaatcac tttacttgct gcaacatccg cagtcacaag attatcagta aggtaactca
    10861 aaatttcttc ttcaagctct tcatcttcta agatcatcaa gtggccttcg aaaatcgcct
    10921 ctttttcctc acctaatgta cggcgagctt tttctttaat cgcggttaat tgagcagctg
    10981 ctttttcgcg gcctgtaaag aattttgctt tttctgcctc aacttcgctt tcagtaattt
    11041 tgcgagtatt tagtacaatc ggctcttctt ttaaaaccaa tgcttttcca aatactgcac
    11101 caggggaagc tgcgatacct gtaatcataa ttgtgtcctt aatttaagat gatgtaaaaa
    11161 tcatctggat gttacatagc aaaacacttt attgtagctt attaagctac aataaagtga
    11221 cataaatctt aaatccgaga atagaaaaat tattctaaag taggaatcaa atcaactaag
    11281 aaatcaactg ctttttgctc atcttcgccg tccgcagaaa ttgtaattgt tgtgccttga
    11341 gtcaaaccta atgtttgtaa tttgaataag ctttttgcac ttgcactttt accacctgaa
    11401 gtcacagtga tatcagaagc aaaaccttta gctgctttta caaattcagc cgctgggcga
    11461 gtgtgtaaac catttggagc ggtaataaca acatcttttg agtacataaa atcttcctct
    11521 taaatttgaa agttaaaaaa atttgtttta atccatttat tttgcgcacg caactttact
    11581 acaaattcaa attaaaaaca cgctaaaaag cgcaaaaact cgtcgtatca ccctgctaaa
    11641 tagggtgttt tttaagctaa cttaaaaatc atttgtcgaa tatcaagatt ctagcacaca
    11701 cttaagcgga aatgttacaa taatttcgct accacttctt tcacttatat tctaaatttg
    11761 gagcaatatg cttagcacat taaccaaaac aaccgcatgg tttatctact ttagtattat
    11821 tgctctctat gtttttagtc tatgggcgtt aatggactat caccaaaatg ataccttatt
    11881 tgttttttcc gatataacgg ccacggtcgc ttatagccta cttcaagcca gcctctctgc
    11941 ggtaatttca ctatttttag gcagcctact ggcaagatct ttcttttatt tggactttaa
    12001 aggaaaaaat ttactctata aaaccatttc gtttatctgg gcattaccct cactagtaat
    12061 tatttttgcg gtaattggcg tatttggtaa ctcaggctgg ctagccaaac ttttccaaca
    12121 actcggttgg aattggcaat ttaatttata cggcttgcac ggcatcattt tggctcactg
    12181 cctgtttaac attccgtttg tgatgaaata ttataccgga gggcttcaat tgactccaag
    12241 cagtcgcttt cagcttgcgg ctcaattaaa cctaacaggt tggaattata tccaaattgt
    12301 ggaactaccg ataatcaaaa ctttgctgcc ttatatgttt atgactgttt ttctggtctg
    12361 ctttaccagt tttccaatcg tattaatgtt aggcggtagc ccaaaataca gcacgcttga
    12421 agttgccatt tatcaagcgg tcacttttga atttgatttt gcaaaagccc tcatcttaat
    12481 tggggtacaa tttgtagtgg gcattggctt acaattagta atgaattata tctcgacttc
    12541 tgcactcaaa aagctaaaac agcaacctaa ccgagtagaa atttggaaac caaagcctac
    12601 cggactggct aaactcggct tacaagcggt tatttttttg caaagttttg caataattct
    12661 accgcttgtc agcgtcattt gggcaggact aagcgtgtca aaactcagtg agcgtttgct
    12721 caaccctact ttatggcaag ctactcagta ttctttatac ttaagtttaa ttgcttctat
    12781 attcgtaatc acaatcagct atttgattgc acttgaagct cgccaattac tctatacaaa
    12841 agccaagctc agatatagca ttctcaccgc aatcacccta ttccccttac ttctgccgat
    12901 ctttttatta tcggttggtt tgtttatttt attaatgaat agtgaactct caagcgaggc
    12961 attactgcta ctggtcggga tatgcaacgg cttaacatta ctgccattta tttacccaat
    13021 gcttttctcc gcaatgtgga atagcctcac ctcgcacgac aaattagcac aaggacttgg
    13081 gctaaagggg cttcgccgtt ggtggattgt ggaaaaaaat caactaattc gaccgcttgt
    13141 taccgtattt gcgttagcaa tgtcatcgag cttaggcagt ttcaccgtca ttgctttttt
    13201 cggcaacgct gatttcagct cactccctta tttactctac caacaacttg gtagctatag
    13261 aaccgaagat gctgccgtta ccgcactcat tctgatgtta tttgcgttac taccattctt
    13321 attcagcaag gataaactat gatcgaactg gatctccatt tcacttacga acaaatgaat
    13381 atgcactttc agctttctgt caaaaaaggc gaacgtattg cagtggttgg cgaaagtggc
    13441 gcaggcaaaa gcacattact taatctaatt gccggttttg aaaaggcaga aagtcagcaa
    13501 agccagctgt ggctaaatca gaaaaatcat cttgataccg aagttgcaca acgtcctgtt
    13561 tcaatgctat tccaagataa caatattttc ccgcacttaa cggtagaaca aaacatcggc
    13621 ttagctttag taccaagttt gtcgctcaat ccgaaacaaa aagcacaggt taaacaaatt
    13681 gccacacaaa tggggattga aaccttcctt cagcgtcaag ccgatcagct atcgggcgga
    13741 caaaaacagc gagtggcatt agctcgtact ttgttgcggg ataagccgat tttattattg
    13801 gacgaaccct tctccgccct cgacccgcaa cgccgagccg aattacagca aatcgtgttt
    13861 gacatttgcc aaagcagaaa tctaaccctg ttaatggtaa ctcaccaact atccgaaagc
    13921 ctatttctat ttaatcgggt gataacggta gcagatgggg agattgtttc tgatctctcc
    13981 cctctttagc aaagcagatc tgctaggttc taaatgtagg gacgccacgc tggcgtacgc
    14041 aacgatgtca attccaacaa tctaacggtg aatgatgttc cgcacggata ctagcgttat
    14101 tcattttttt cagaactcgc cccctacttt agcaaagagg ggaactgtaa ctaatattac
    14161 ctccctattc cgctattctc ttccccaaaa tgtgatctac ttcacaaatt tggaaatatt
    14221 ttaaatcttc tcttttttag ctaaaccgat ttagttaaaa tgcaaccgat tattttttat
    14281 gaaaggtaaa ttcaaatgaa ctatccaaaa attgcagaac aaacgattgc aaaattaggt
    14341 ggcaaagaaa acattaccac acttgcccac tgtgcaaccc gtttacgctt aaccattgct
    14401 gatgaatcca aaattgataa agaggcgatt gaaaatattg aaggcgtgaa aggtcaattc
    14461 tctactcccg gtcaatacca aattattttt ggatcaggca cggtaaatga agtgtatgcc
    14521 caaatgcaaa aacaaatggg tatggcagat atgtccacct ctgaagtagc ggctgccggt
    14581 gcagcaaatc agccattact acaacgtttg gtaaaaggct tagccgatat tttcgtgccg
    14641 atcattccgg ctattgtggc tggcggtttg ttaatgggta ttcactcaat gctaacggca
    14701 gtcggtttct tctgggaagg tgaatctgtt gttagccgtt atccgaatat ttccgattta
    14761 gttgatttca tcaacactat tgctaacgca ccatttgtat tcttacctgt attactcggt
    14821 ttctcagcta cacgcaaatt tggcggtaat ccattcttag gtgcggcact tggtatgtta
    14881 ttggttcacc cggcacttgc agacggttgg aactatgcca aaaccttaat ggaaggtaac
    14941 atcaaatact ggaatatttt cggcttagaa attgaaaaag tgggttatca aggcacggtt
    15001 attccaacta tcgtttcggc ttgggtgtta gcaacccttg agaaaacttt ccgtaaattt
    15061 gtgccaagct atttagataa cttggtgacg ccaatgatgt cattatttat cgccggtatt
    15121 ttagctttca ctgtaatcgg accattgggt cgtgaagcag gttcagcgat ttcagcaggc
    15181 ttaacgtggt tatatgacag cttaggcttt atcggtggag caattttcgg tacattctac
    15241 gctccaatcg tgattaccgg tatgcaccaa acctttattg cggttgaaac ccaattatta
    15301 gctgaagtag cacaaaccgg cggtacattt atcttcccga ttgcagcaat gtctaacatt
    15361 gcacaaggtg cagcctgttt aggtgtagct tttgcaatga aagatgccaa agtgcgtggt
    15421 ttagccgtgc cgtcaggtat ttcagccctg ttaggtatca accggcaatg ttcggagtaa
    15481 atttacgcta ccgccaagcc tttattgcgg cgatgatcgg ttcaggttta gccagtgcat
    15541 ttatcgcttt ctttaatgtg aaagcaattg ccttaggtgc agccggtttc ttaggtattc
    15601 cgtcgattaa acctgacagc cttgcaatgt attctgtcgg tatggtaatt tcatttgtag
    15661 ttgcatttac catttcagcc atttgggtga aaaaagcaca agcgaaataa tcaaaaatag
    15721 atcacaaaaa agggattaag tgttagctta atcccttttg tttttaaacg ccttagctta
    15781 acaatgagtt tcgcaagcgg tcatttttcg ctaaaaattt gcaaattagc tgttgtgcat
    15841 aattcgagaa atacgccctt cggcaatctc ttcatcacga attgtcagca cttcacagcc
    15901 gtctttggta actacaattt gatgctcata ttgtgcggag tggctgcggt ctttggtttt
    15961 cactgtccaa ccgtcaccca ttaagcggac ttcttttttg ccggcattca ccattggctc
    16021 aatcgtaaac accattcctt cttttaacac tacaccgcca tcatcggtag cataatgcac
    16081 tacttgtggg tcgcaatgga attcagtacc aataccgtgt ccgcaatatt ctcgtactac
    16141 cgaaaagcct tctttttcca catatttttg aatcgctgta ccaatttctt ttaaacgcac
    16201 ccccgctttc acggtgcgaa tcccgacata aagggactct agcgtaaccg ccattaagcg
    16261 tttatcccgc acacttggct caccgactac atacatcatc gagttatcgc catagtagcc
    16321 atctttgata accgtcacat cgatgtttaa aatatcgcct tttttcagct ttttgtcatc
    16381 acttggaata ccgtgacaaa cgacctcatt taacgaaatg cacaccgatt ttgggaagcc
    16441 gtgatagccc aaactggctg aagttgcccc atttgccaaa atgtattcgt gacaaatttt
    16501 atctaattca ccagttgtta cgccctcttt cacataaggg gcgatcatta ctaaaacatc
    16561 tgaagccagt ttacaggcta ctcgtagttt ttgaatttct tcttcgtttc ttaatggaat
    16621 attattcatt acattgctct ctgctatatc tgccataaaa agtaagggga ttataccttt
    16681 tttcagcaca atccccaaac ctcagtttag atttctactt taaatcaagt ttcttcataa
    16741 tgaaatttaa tatcacacca taaagcggta gaaatagcag taggctcaca aagagtttaa
    16801 acgcataatc gaccgaacca atagtaaacc aatgctctgc catatattca tcgctacttt
    16861 tataaaaggc gatagcaaag aatacaaaag tatcgattaa agtaccaaaa atggttgaac
    16921 aggttggagc aagccaccaa cgcttacctt gtcttaaact attaaatact agaacgtcta
    16981 ataattgccc aactaaataa gctgcaaagc tagccagtgc aatacggaaa acaaaaaagt
    17041 tgaactcagt taatgcacta aagccttgaa attgggtttc aaagaataaa acggaaatca
    17101 catagcttat gacaagtgcc ggaaacatca caacaaaaat aatttttcta gcaagatctg
    17161 caccaaatac acgcacggtt aaatcggtcg ctaagaaaat aaatgggaat gttaacgttc
    17221 cccaagttgt cggaaagcta aattctgtta gtggtacttg aacttcaaag ctaatttgca
    17281 ctaaatagtt gctaattgcg atgataaaaa tatggaaaag tgaaagtaaa attagagaac
    17341 gacgtttgtt ctcatccgaa aaataagtag taaagttcat attgtatcct ttttcgttaa
    17401 atgaaaactt gaggtaaggg aactcaaggg ggcattattc tatacatttt cccataaaaa
    17461 tcaagctcat attatttcaa aaaatgaaac aattaatcta ttaaattagg gtttatctat
    17521 ttttgacata aagtaaaatg gcacaactta acacttacat aaggaataga taatgttaca
    17581 actgagaaaa gccaatgaac gaggcgatgg ttcttttagc tggcttaaaa gctaccacac
    17641 tttttccttt gccaattatt acgacctaaa acatatccat ttttcacatt tacgggtaat
    17701 taacgaagat ttcattgcac ctaatcacgg ttttggcaag cacccgcacg ataatatgga
    17761 aattttaact tatatgttaa aaggacgtgt ggctcacgaa gacagtatgg gtaataaaac
    17821 cgaagtaaaa gctggcgaat ttcaaattat gtctgccgga accggggttt tccattcaga
    17881 aattaatcca gatccgaatg aaaccttaca cctttaccag atttggatta tcccaaacca
    17941 aaaagatgtg acccctcgct acaaccaaga tagatttgcc ccgaaagagg gagcaacctt
    18001 gattctttcg ccaaacgcag aagaaggttc atttaaaatt taccaagata tgaagctgtg
    18061 gcgttatcaa tacccaacag ccaaatcaga gcaactggca ttagagccaa accgcagtta
    18121 ctggctacaa gtggtgaaag ggaatctaaa catcaatggc gttgaacttt ccaccagcga
    18181 tgccttaggc attcgtcaag agaaagcact tgaaataaac actcaagatg acgtggaatt
    18241 tttactgttt gatctagtgt aaatacaaga gacaagcggt taaatttgta aaaaatatta
    18301 ctaattcagc tgcttgctct cattaaacat tatcgcacca aaattctcac tcctagcgtt
    18361 tcattttgtt gaagtaaggt atttatccgt gctgtttcca cacataccat attttgatac
    18421 gccgtttctg tcatcgcact aacggtatta tgccaaggat tccaaagtac ggtttcggta
    18481 gcattaatat gctcaacttt aatggtgcgc tgatttgcaa aatcttgaat aaaattcacc
    18541 gcttgtgccg gataaataca atccacattt tcacttatct tgcgaggtga tggcacttca
    18601 atttcttgat ttgtcaattt atcaaaacat tgtgttgcta agccttgtac ttccgttttc
    18661 gtaatatcac cgacctgaaa ataagtatgc aatgcagcct gtgccggctc cggtgctaag
    18721 tgagtaaaat acaactcaca gacttcgcct agtatcatct cgagttttgc ctgattttct
    18781 aaccccaaaa caacacgaat cccatctgct gaggaagtat gttccagcaa attccacgct
    18841 tgaatacggg ctgtgccgtg agcaggcttt ttgtctgtac caaaccaagg gtggcaaatc
    18901 ggaatgccgc cacgaatcgc cacacctgtt tggaaaggtt caatttcact taaccataat
    18961 acatcttgct gagtattttt aggttgccaa ctcagtaact gcgcaccttg caatgcaatt
    19021 ttagccttca ctttggagtg attaatctct aaaacatcca attcattata ttttgctaat
    19081 gctatttcag ggctgatcat tttgattcgt agattattca tcgtattgct cctcatcttg
    19141 aaagataaaa tgtgaagtgg atcacagaat taaagcttaa accgctacct ctttaaaggt
    19201 aatcgttata ataagggcat tctaacatag cgaaggagaa aaaaatgaaa acactcggcg
    19261 aatttattat tgaaaaacaa gcagaatacc caggggcaaa aggggaatta agtgggattt
    19321 tatcttctat tcgtttagcc gctaaaatta ttcatcgaga aatcaaccgt gccggtttaa
    19381 gtcaagatat tttaggtact gcaggttctg aaaatgtaca gggtgaagcc caaatgaaac
    19441 ttgatgtatt cgccaacgaa acgatgaaaa aagcgttact tgcacgtgaa gatgttgccg
    19501 gctttgcatc ggaagaagat gataattttg ttgcctttga taatgagcgt gggagaaatg
    19561 caaaatatat tttaatgacc gatccgcttg atggctcctc caatattgaa gtgaatgttt
    19621 ctgtgggaac aatcttctct atttaccgcc gtgtctcccc tatcggcacc ccagtaacac
    19681 aagaagattt cttgcaggaa gggcgtaaac aagttgccgc cggttatgtt acttatggtt
    19741 cttcaaccat gctggtttac accacaggca atggcgtgaa cggttttacc tatgatccgt
    19801 cattaggctt atttattctt tcacaccctg atctaaaaac ccctaccgaa ggaaaatatt
    19861 actccatcaa tgaagggcag tatgttactt tcccacaagg tgtgaaacgc tttattaagt
    19921 attgccaaga aaccgatgaa gccatcaaac gtccatactc ttcacgctat atcggctcgc
    19981 tggtgtccga tttccaccgc aatttattaa aaggcggtat ttatatttac ccaacctcca
    20041 ccgtatatcc aaaagggaaa ttgcgtttac tctatgaatg caacccgatt gcgtttcttg
    20101 ccgaacaagc cggaggaatg gcgacagatg gtattaatcc gattttagat attaagccaa
    20161 cagagctaca ccagcgtgta ccgttctttg tcggttcaac ctcaatggtc aaacaagcgg
    20221 gcgaatttat gcaagaattt gcaaaataat gctcctgctt atcactcaat aaaactttca
    20281 acaaagcagc tgcgtattcc gtagctgctt ttgcctatct taagattgta aatttgtata
    20341 aaaaacaggt aagacctctt gccaaataaa taattatcta tagaataccc caccttttta
    20401 ctccattaat attagcaatt tattaacatt ttatgataaa taaaaacttt ccactttctt
    20461 accttgccag ctttgtggca tttgctgtta gtttatctgc aacagcctct gttttcagag
    20521 atgatatcga tcttcaatac taccgtgatt ttgcggagaa taagggtaaa ttcaccgtag
    20581 gtgcaagcaa tattgaaatt aaagataaaa gtggcaatac attagatgca ctcttcccaa
    20641 atgtaccgat gccagatttt agtgctgcaa accgtaactt agggatagca actctagttg
    20701 caccgcaata tttagtcagc gttgcccata atacacaata caatactgtg gaatttggcg
    20761 ccccaggcac taatgccgat gcacaccact acacttacaa agtcgttgat cgtaacgact
    20821 atggaattgt tgaaggtggg caacatcaag attatcaagt gccacgtctc aataaattag
    20881 tcactgaggt cgcacctgca acagtgactg atctgggtaa taatgcttca gcctaccaag
    20941 atagtagtcg ttttactcac tttgctcgct taggctctgg tcgtcaaata gtcaaaaata
    21001 ccgagcataa agacaaccaa atttcaacat cttaccaata tttaaccggg ggagttcatt
    21061 taccaatcgc ggtacataat tccgattact ggctagattt tagaggaaat gcattaaaca
    21121 atagcccata tggagcatta actgcatttg gtactcgtgg tgatagcggt tcaggtgtgt
    21181 atggctacga tcagaaaacg aaacgctggc tactactggc tacttacacc tttggtacgc
    21241 ctgcaaacaa ctactacaac cgtgccggta ttatccgtca ggattatcac gataaacaat
    21301 ttgccgaaga tattgccggc acattaacca atgcgcaaca aaatgccgtt tttgaatggt
    21361 ctgcacaggg taaagacagt agcattggta ataaagggaa aaacttaacg gtttcgcttg
    21421 ctgatcgttc aattaaagac aatagcgctt ctccattagg ctatggtggc acacctcagc
    21481 tacaacttcc gcaagataac accggaaaaa ccctaaacat tgaaggacaa gatagcacta
    21541 tcgtattaaa aaccgatatt gaccaaggtg ctggcgcatt aaatttcaat gctaatgcaa
    21601 cagttcgccc tgaaaatgat caaagctggg taggagctgg aattgtagtg gcaaaaggta
    21661 aacaagttaa ttggcaagtc aaaaacccac aaggtgaccg tttatctaaa ttgggcgaag
    21721 ggacactaca tattaacggt aaaggcgaaa accttggtga cattagtgtt ggacaaggca
    21781 ctgtcatttt gacccaacaa gcgggtgaaa atggcaaaaa atctgcattt aaccaagttg
    21841 gtattgtgag cggccgttca accgttgtat taaacagtgc tgatcaagtc gatccaaata
    21901 agatttactt tggcttccgt ggtggtcgtt tagatctcaa cggcaacaac attgcgttta
    21961 atcgtattca aaatagtgat gatggtgcgc gtattgttaa taatcaccta caaaaagctg
    22021 caacactcac cattaatggc ccgaagcccc ctgaagcaac ggacttaaaa tggggaacgt
    22081 ggaaagaaaa cagtgctgat atttatgaat atatcaaccc acatgctaat aaccgtaccg
    22141 attactttac gctcaaaggc aatccaaacc aatatatgcc aacgaatggc gcaagtaatg
    22201 cccattggac cttcctgtct tctaacaagg atgcagcagt aaaacaagta ctcgcgcaaa
    22261 aaggtctgga gcatcgctac aatagcttta atggttttat tggcgaaaca gataacaccc
    22321 agcataacgg tcgtttgaat gtggtgtatg atccaaaagc ctctacacca ccggcaactg
    22381 cctctgaagt aacttggggt aaggggctag tagctggtgc agatatttat ctgttcacca
    22441 atccgaaaac gaaaattcgg gaatactttg cattaaaagg tgatccaaaa caacctgttc
    22501 ctcgcggtgg cttaagcagc gagcattggg aatttttagc tgcagatcgc aatcaagcga
    22561 tcaacaaagt attagcccgt aaaaacgcac caatagagca agaaaagctc aatagtattt
    22621 ataccttctc tggtggtttg aacttaaacg gcgatctaac tgtgaaagga ggtaaagtgc
    22681 tactttccgg cagaccgacc cctcacgctt atgatgcgat caataagcaa gatgtagttt
    22741 atgccgatga ttggcaaaat cgtcaattta aggctgataa tatgcagcta aaccaatacg
    22801 ctcagcttta tgttggtcgt aatgtctcaa acctgcaagc aaacttgtca gcgaatgacc
    22861 atgctcagct acatttaggc tttatcaacg agcaaactcc aagttgctac tattctgagt
    22921 acactggtaa aacaagctgt gatacacaag cggtcgtttc taatgaaatt tttgcaacac
    22981 tgcccacaac acaaattaat ggtgatgtca gcttggcaga acagagccaa ttacacatcg
    23041 gcaaagcgaa cctgattggc accattcaag cagcagcaac aacgagcatt cgtttagcta
    23101 accaagcaag ctggacaaac actggggata gccgtactgg caatttagtg gcagaaaacg
    23161 gttcaaccat caacctgaat gaaaaatttg taacaggcga gataccgact cgttttaata
    23221 cgttgattat tgacggtaat ttccaaggta atgccaaaat caactactta accgatattg
    23281 ctgctggcaa aggcgatcac ctacaagtaa atggtctcgc cgaggggact ttcactcttg
    23341 cattacgcaa cagtggcaaa gaagctgaag tggttagccc gttaagttta ttaaccttaa
    23401 cccatcaggc tcaggctgat aaagctaaag tttcactgga aaacggttat gttgatttag
    23461 gtgcatatcg ttatgtgctg gcaaatcgaa gcaacggcta tcgcttatac aatccgttaa
    23521 aagatgctaa agatcggaat cagtcgattg caacggcgaa agcggaatta gaccatgcta
    23581 ttgcagaagc ggataaacaa aaacaggaaa tcagccgttt gaacgcagaa gccgagaaag
    23641 agcgccaagc agagaaaaat gctaaacaag ccgctgcaaa cgcacaatct cagcttagcc
    23701 aagcaaatag cgaattaacc cgtttgcaac aatatgctga ttactaccgc cgttactatc
    23761 caacgtatta ccgccaaatc caagggcaaa ttacggtagc aaaacagaaa gtcacacaag
    23821 cgagtgctgc actcactaca gcagaaaaca atgctaaagc atccgctgcg caaattgcta
    23881 acgtagaaca agcggttgaa actgcacaaa atattgcaaa acaagtagaa gcaaaattag
    23941 ctaccttacg tataactgct ggaaatagtg aagcaatttt aaacgctgag gcactaaaac
    24001 tttgccaaga gaatggtgca aattgtgagc atctacaagc acacgcagac actgacagta
    24061 gtaatattca acaaagcgac tgggtaagcc aatatgccaa taccgctctt tccgagctat
    24121 cggcacaggc aaattctgcg ttgcaaatcg ggcaagactt agatcgccaa ctgttcgcaa
    24181 aacacgataa attccacgtt tggagcagta tcgaacatca aaaaaccgaa caccaatcgg
    24241 atttatatcg cccgtatgag caacaaacca acctcactca gttaggcata gaattaccgc
    24301 ttgcaaacgg tatcaatgcc ggtgtgatgc tatcccgcaa ccacgctaat gcggaatttg
    24361 atgagggagt gaacggtaaa tccaacttac tgatggcaag cctatatggt aaatggcatt
    24421 cggaaaacgg cactttcgtc agcctagacg gtagctacgg taaagcgaaa aaccgtatcg
    24481 acctgttcgg ggagaaccgc tttaaccgtc atattatggc gattggggca aatcttggac
    24541 ataacttcga cttagccggc gtacaagttc agcctgctgt tggtacacgt tactatcgct
    24601 tcagcgcaca aaattacaaa ctgggtgaag tagaagtccg cagcccgaaa gcacacttta
    24661 tggcctacca agcaggtgtg aaagtcagca aggcctttga cctctccagc tggaaagttg
    24721 agcctagcct tgccgcacat tatgtcgatg ccagcagcaa acgcttgagt gtggcagtca
    24781 atgacaatac ctttagccaa cgtttcggac gttatctgaa aactgaagtg ggcgtgggtg
    24841 ttacaaaagg tcaatggcaa ttaagctctc accttggtct attgaaaggt aacgaaatcg
    24901 gcaaacaaca ttttgccggc ttcaaactgg gatatagctg gtaattgcta ttagccccta
    24961 aactaagcaa tcgcttactt taggggcttt ttagtcaatt aattagccgg ctctttgctt
    25021 gcctctagcc actgccgaat aaaatcagca agctgttcaa catcattaat atccaaccga
    25081 ttttcccgct caagcggata atcggttgcg gtcgcgattg tccactggtc taacgccggc
    25141 aacggttttt caatttcttt acggtgtagt tgaattttcg gcattgtttc gtgcttaaag
    25201 ccttccacta aaactaaatc taccgtctct gggtcaaatt ttgcaattaa ttgtgaaaat
    25261 tcgaccgctt gtttagtttc tgctgcctgc ttagtttccg tcattaatgc ccaacgttca
    25321 tcacacacga ccatagtcgg gtttgctcct gcttctcgca agcgatagct gtcttttcct
    25381 tttttatcaa tatcaacatt gtggtgggaa tgcttaatca agccaacccg aataccgcaa
    25441 gcggtcaatt ttgggattaa tttttccaat aaggtggttt tgcctgtgcc gctatagcca
    25501 gtaatcgcca gggttttgat gggatatgga ttgaaagccg ttaaatcttc taaacaatta
    25561 aaattgcgaa atgcggcttt atatgccgag aaatccactg ccacactttt ttgggtttga
    25621 aaaaaccaca ataatcggcg ttcgccggag gctaaatacg ctcttaaatt ttccgccacc
    25681 gaacgatgca ccaaggcaac cgttgggtgt gggcggtcgc catcgtgagc ataggcaatt
    25741 tgagcctgat taatgcgtaa tgccgtgtgt aacttgtgca atagttgggt tggaataaac
    25801 ggcgtgtcac aaggcacaaa cagtaaatag tcgctctcaa tctgctcgaa cccggaaagc
    25861 attccactta gtgggccttg aaaatcgggc aagctgtctt ggtaataagg taattcaggg
    25921 taaagttgtt gatattgttc caccgaacga ttgatattga gccaaatatc ctcgatttgt
    25981 ggcgaaagtc gggctaacag gtgcgaaatc aacggttttt gatctaacag ttgcaaccct
    26041 ttttccgctc cattcattcg ccgagccaaa cctccggcta aaattaccgc tgaaattttc
    26101 tcttccattt ccctctccaa acctgaattt ttttgccaaa tgagggctaa aagtatatta
    26161 ttccgcttac tattcaacaa gttttttaag aggcatacta tgaaatgcaa acgtttagaa
    26221 gagctattgg aactgcttgg cgaacattgg cggaaaaacc ccgatcttca cttaattgat
    26281 attctgcaac agctttcggt ggaagtaagc gagccggata attttcatgc cctgcgtgac
    26341 gaggttttga tttatcagct caaaatgcga aacgccgata aatttgagcc gattccgggc
    26401 attaaaaaag attatgaaga tgattttaaa accgcattgc tccgagctcg tggaatttta
    26461 aacgattaag tttgtcttat ttctcactta tcatattttt gaaggaatca ttatgaaaaa
    26521 atttgcatta aaaaccgtac tggttgctct ttctgccctt tttgcattta acgcaaccgc
    26581 agttgcagca gatccaactg ccggcaaaga gtatattgag gtgcgtaaag ccccatcggc
    26641 tcaaaaagaa gtggtggaat ttttctcatt ctactgcccc cactgttacg attttgagct
    26701 aaattacaaa attccaagcc aaatcaaagc gaaattgcct gcagacagca aattagtgca
    26761 atatcacgtc aatttcttag gtcgccaatc tgaaaactta acccgtgctt gggcattggc
    26821 aatggcatta ggagcggaag ataaagtaaa aaccgccttg tttgaagccg ctcaaaaaga
    26881 tgcgtttaaa tcaatggacg atattcgtgc ggtcttcatc gctaacagca ttagtgcaga
    26941 acagtttgac agcggtatca acagctttgc agtaaacggt ttagtcaata aacaagttca
    27001 attagctgaa gacttccaaa ttcgtggtgt accggcgttt ttcgtgaatg gtcaatacca
    27061 gcttaatcta gaaggatttt ccgattcaag ctcgaccaat gattttatcc aacgctatat
    27121 tgacgctgtg gtcttcttga gtaagaaata atcgtcccca tattaaggag atggttttac
    27181 aagtgctgaa tagtaaagct attcagcctt ttttatatgc aaactcggag aacaaaatga
    27241 caaacttatc cattctcaac cttgtgccga ttcgtgaagg gcaaactgcc aaacaagcgg
    27301 tcgattctat ggtgaaatta gcaaaacacg cagaaaatat cggcattgaa cgctattgga
    27361 ttgccgaaca ccataatatg aaaaatcttg ccagttccgc cacgcaatta ttgattcaac
    27421 acgccctctc ccacaccgaa aaattaagag tcggctccgg tggcgtgatg ttacccaacc
    27481 acagccctta tattgtggca gaacaatacg gcacgcttga aacactctac ccaaaccgag
    27541 tagaactggg gcttgggcga gcccccggca ccgatatgcg aaccgcctct gccttacgcc
    27601 cagagtttgc ctttccccga tgaaatcagc gaattaaaag gctactttga cggtacaaac
    27661 cctgtttccg cctatccggc acaagggcta aatgtgccgt tttatattct aggctcaagc
    27721 cctgaaagtg cctatttagc ggctgaacga ggcttgcctt acgccttcgc ttcacacttt
    27781 gcccctcgct ttatggacga tgcaatccgc atttaccgct cacattttaa gccctctgat
    27841 gtgttagata aaccttatgt gattttaggt gtgaatgcca ttgtggcaga aaccgatgaa
    27901 gaagcaacac aactggcgac cacccaaacc cagtttttct taaatgtagt gaccaatgcc
    27961 caacaaaatc tgcaaccacc gctcgaatct gacgacaaag tgtgggaaaa ttttgtgaaa
    28021 gcagaaattg tgccgcattt cgggccggtt gcctttgagc aaaatgctct ataccgacaa
    28081 gagaaaaccg ttgtccgcca aatgaccgaa tgctccttaa ttggtagccc gcaaagcgtt
    28141 gaacaacaac ttaaagccct aaaagcacga gtggaaattg atgaaattat ggcggtaaac
    28201 tacattttcg atgagcaaaa acagcatcaa tcctacactt ggttaaaaca aattacggat
    28261 aaggtgtaat atgagcaaaa cgatgcaaca aaaagcggct gaggcacata atatgtgccg
    28321 gctaaaaggc gattctatgt tggataatgc agaccgccaa attgcctttg aagcggtgta
    28381 tgagagcgaa gaactggctc aacaagcggt cgaatttttc gcacaaaaag caaaatcggt
    28441 cgaaaccgaa ccttgtgaaa ttcaaagcga aattacacaa attgcagaag ggtttctgat
    28501 gaaaatgtgg atcactttca gctgccaagc agaagtgatt ctgttccaaa tggcagtaag
    28561 gtagatataa acaagcggtt atttttgatg aaatttttgc aaaaaatctt cagaaaataa
    28621 ccgcttgtat cattatgcct gtttaaccgc agccgattca ctacttggtt tacgccgttt
    28681 accgatattt ttggtatctt tgtggcggac ttttactttt ggttttgccg cttctttttt
    28741 cgcctctttt ttcttcttaa tgcgtgcttt ttctttcttg ctggtcacat ttagctcgcc
    28801 atctttcggg gctttggtgc gaggctctaa gccctcgata atgcgaggtt ttagtacttc
    28861 ttcggtgtaa cgtttaattt tgccgagtaa tttataatcg tgagcttcca ccaatgaaat
    28921 tgccgaacct tttttgccag cacgagcggt acgcccaata cggtgcaagt aagtatccgc
    28981 actataaggt aaatcgtagt tcagcacgaa atcaatatcg tctatatcaa tgcctcgagc
    29041 cgccacatcg gttgccacta acacattcac tacgccttct tttaaacggt taatagcttg
    29101 gttacgttgg gtctgagcca tttcaccttc gagataagta gaacgaattc cccgcttacg
    29161 caaggtttcg ctgagttctc gcacatcttc tcggcggcgg acaaacacaa tcgctctttc
    29221 caccgaaaat tcgctgataa tacgagctaa taatttggtt ttatgctcta cattatcggc
    29281 gtggtaatac cattgctgaa tttttttacg ctcacggcga cttggttcgg catcgacttt
    29341 aatcggatca tttaataatc gctcggcaaa atcctctaat aactcgccct cgagggtcgc
    29401 tgaaaacaac caagtatgct tacgccaacg ggtttctgcc gcaattttct ccgcatcttg
    29461 cccgaagccc atttgcaaca ttctatctgc ttcatcaaaa atcagcactt ccaccgctcg
    29521 gcagtcaaaa ttttcctctt taatgtattg cattaaacga cccggggtag caaccacaat
    29581 atcttgattt ttattaaaga cttcaccgtg attttgatac gccaccccac cggtaatagt
    29641 cgcaatgctc aactgagtaa atttagccag tgcttgggct tgctccgcca cctgcattgc
    29701 cagctctcgg gttggggtaa gaattaaaat gcgaggagcc cctggtttgc ggcgaggata
    29761 atctaataaa tgctgaattg ccggcagtaa aaaagcggcg gttttgccgg taccggtagg
    29821 ggctgaaccg agcagatcac gtccttctaa cgcagccgga atcgtggctt gttgaatagc
    29881 ggttgggcgg ttatagcctt tgtgtgctaa ggctttaagt aattgagggc ttaaatcaag
    29941 ctcctcaaac gtcattaatt gggtcatctc tgtttctcta cttgtaaatt tgccccagta
    30001 tatcacatta actgcttgcc gtgatggtga agaaatttat cctaaaaggt tgaaaaaatt
    30061 gttacactta cacagagccg tttaacggac aattcgctca cttttacttt atagaaggaa
    30121 cattatatgt ttgttgaaat ttatggacgt ttatcttgcc catactgcgt gcgtgcaaaa
    30181 acattagccg agaaaatgaa acaagaatta agcgattttg attttaaatt tattgatatg
    30241 atcgctgaaa atatttcaaa acaagaccta gagcctcgtg ttggcaagcc ggttgcaact
    30301 gtaccacaaa tttttgtgga taataaccac gttggcggtt gcaccgattt ccaagccttt
    30361 gtggaagaga aatttgggat taccgtatag ccaaaagccc gctaacaagc gggctttttt
    30421 atacaaaaat ttgcaaatta ttgttcttct ttcggcagac gtttaatcgc aaaaagtaaa
    30481 ccgccccaga taatcgtcat tgccaccacc atggtaataa ttgctgctga actcattact
    30541 catctccttt tggttcattt ttccacgcca tttttgatag caaataagcc accaccacta
    30601 agccgattgc cattccccag ccgaaggtgt ttacaaacca gcttgggtag ccttcataac
    30661 cttcacgata cactttggcg atttcgctga ataacataaa tgccagtacg ccggaggtaa
    30721 taacgataca taaacgccag ataaagccga ctttgaatga agaaacttcg ttcaggtgtt
    30781 tttcaagggt gcttaatttc tcgctggtga caatcgcaat caaggaaaca aacgccaccg
    30841 caacaatacc gaattggttt acgaatttat ccatcacatc aagcattggt aaaccggtgg
    30901 ttgtaccgaa tagcaagaca gataccacca tcatcggaat gccgactagt agcgtggctt
    30961 tcacacgacc tgaattgagt ttgtcttgca ccgctgaaat aatcacttca attaccgaga
    31021 taaatgaggt taatgccgca aaggtgagtg aaccaaagaa cagcacgcca actaatgccc
    31081 ccattggtgc ttggttaata atggttggga aagcgaagaa tgccaagccg ataccgcctt
    31141 ttgctacctc gttcacttct tgccctgcag ccgttgccat aaagccgagt gccgcaaata
    31201 cgccaatacc cgctaataat tcaaaaccac tgttcgcaaa acccaccact aagcctgaac
    31261 cggttaaatc ggtttctttt ttcaggtatg aagcataggt aatcataatc ccgaagcaga
    31321 tagagagtga gaagaagatt tgaccgtaag cggcaatcca cacacttgga tcagccaatt
    31381 tttcccagtt tggggtaaat aacgcatcta agcctttcgc agcacccggt agggttaatg
    31441 aatagccgac caacactaag aacatcacga tcaactgcgg cattagcagg tttgaggcag
    31501 cagaaatccc ttttttgatc cccatagcca acacagcaat agcaatcagc cataccgcaa
    31561 ttaacggccc tgtaaccata ccgacaaatt caaagctcac gccttgagta atatcgccca
    31621 tttttaagaa ttcaccgata aagaaatcca ccggtttatc gccccacgct ccgctgaatg
    31681 agaagtaggt gtaacttgct gcccagccga gtaccaccgc ataataaatc ccgatgatca
    31741 cgttgatcag cacttgccac caaccgaagg tttcaaattt cgagctgaaa cggcggaaag
    31801 cgagcggtgc accaccacga tatttatgcc cgatagaata atctaagaac agcagcggta
    31861 aacctgcggt caaaagggca atgatatacg gcagaataaa agccccaccg ccgttttcat
    31921 aagtagtata agggaaacgc cagatgttcc ctaagccgac agcggaacca atagccgcca
    31981 taataaatgc accacgtccg gagaacgttt ctctttgcgg tgcgtttgat gattgattag
    32041 acacggtaaa tacctcaaac tgataaagtt ttatataaaa aagaattgag ttaaatgtaa
    32101 attaatcaca ctttaagtgc ggaatatgcc caatttcagg ttgaatgtaa agtagaaaaa
    32161 tatagaaaaa cagataaaaa acgctcaatt ttcaatataa aactccaaat aaaaacaaaa
    32221 attcaaacaa aaaaactaaa aacaagacat cttcagttaa atataaataa aaacctaaaa
    32281 gctaccagca taattattta tattaaaatt aacaaaaaaa taacacatta tttgtacaaa
    32341 taatgtgtta tttactttct tttttcttaa aattaggctg aatcttgctc tttttcaatt
    32401 ttcggtgaaa gatgttcaac tttcgcttgt ttaaccatat tttcactcac ttcaagaatc
    32461 atcactttca agttgtagag ttcaaattcc attccttcat caggaatttt ttctaaatgc
    32521 tctaaaatca agccgttaaa ggttctggct tcctcaagcg gtaaatccca atcaaatagt
    32581 ttgttcagat cacgcaggtt ggctgagcct tcaataatca ccgtaccatc tgattgttgc
    32641 tgaacttctt gctcaagggt cggtgctgat gaagtcgtaa attcacccac aatttcttct
    32701 aaaatatctt caagagtgac tagcccttta atatcaccat actcatcaac cactaaacca
    32761 atacgctctt tattcgcttt aaagttcatt aattgtgtag tgagtggtgt gccttcagga
    32821 ataaaataga cttcatctgt tgcacgcagt agcatttctt tggtaaattc gtttttctcc
    32881 aacattaaac gaaaggcttc acgcacccgc aaaatcccaa caatattgtt gtccatatcc
    32941 cctttgtaga taatcacccg agcgtgaggg gcgtgattaa gctgacgcat aatcgctttc
    33001 cagtcatcat caatatcaat gccgctgatg tcgttgcgag gcaccataat gtcatcaacc
    33061 gtcacttttt ccatatctaa aatagagacc aacatttcac ggtgttcggc aggaataaat
    33121 ttaccggcat caagcactac actacgcagc tcttcggagc ttaagccttg tttatctttt
    33181 tgttgaatcc gcaataattt catcagcccg gcaataatca aattcattaa aaatacaatc
    33241 gggctgagta attttttaag cggtgttagc acaaagctgg cgaaaaaacc gactttttca
    33301 gggtaaattg ccgctactgt tttaggtagg atttccgaaa aaaccaacat tacaaaagtt
    33361 agcaaaccgg ttgcaattgc cacaccggct tcaccggata actgcatacc gatcatcgtt
    33421 gcaatcgcag aggcggcaat gttgactaag ttattacaaa ttaagataag gctaagtagc
    33481 acatcggtgc gtgccaagag tttttccgcc agtttcgccc ctttatgccc ttgctcggca
    33541 aggtgacgca ttttgtagcg gtttaaagac atcaagccgg tttccgagcc tgaaaaaaat
    33601 gcagagagta ctaaaagaat agctaaggta ataaataaac tactcagggg aatactgtcc
    33661 aaaattcaat tcctatgtaa attaatcata aaaggtagcg taacactaaa agcgttgtca
    33721 ctacctcact ttgtcggttc agccgacttg caaaaaattt tgcttttttg accgcttgta
    33781 gatcattaat tgacgatcaa acgactaccg aaatagccga tggtgagcaa catcataccc
    33841 gaaatcgaat aaataagcac ccgttttcca cgccaatgta atttccactg tccaagtaac
    33901 tgaatgctat aaacaagcca agctaaaaag gagaaaattg ctttatgaat atgctccggc
    33961 tcgaaaaagt tatgcaaata aaccacaccg gaaaataaag tgagcgtcag catagcttgt
    34021 gcagctaagg ttaaagtaaa gaaatggcgt tcaaccgtca ttagcggtgg tagaatctgg
    34081 caaaagaaca ttgttttgct ctttagtttt ctatccagcc atttgagttg aaaagcataa
    34141 attaatgcta aaaagaagag agcataagaa aataatgcaa tccctaaatg gaaaagcagt
    34201 cccgcatttt cttgcacatt acggataaaa ctacctttta caaatccagc cacccccaca
    34261 ctacaaatgg cgaaagagta cacaataatt aaaggaaacc aaaccgtttt ccattttaac
    34321 aacgcaagtg tgacacaagc cgccataatg aaactcatca aagagattac atttgttacc
    34381 gtaaaattct gcccttcgat taaaaataat tcattatata acacagaaaa atgcaaaatc
    34441 gtggcaagta acccaaaccc aaagactaac atcatattag gctttttatt aactgttgcg
    34501 gcattttcta aattcactag agtaggggca acccaaagta gcgctagagc gtaagcaaca
    34561 atggataaaa gaggaagtaa catattgtct cgaatctcgt caaataagaa taactatcag
    34621 aattttaatc aaaagtaacc gttttagcta gtttttgctg ttgctagatc aaaaattatc
    34681 ctaaaaaatg tgcttttcgc cttaaattga accctacctt ttctcggtaa gattatctca
    34741 ctgaaaacaa taattttcct ttgactaatt tgctttctat ctgtaatatg cacaccctat
    34801 gcaaaaggta aaactacccc ttacgattga cccttacaaa gatgctcagc gtcgcgttga
    34861 ttatgaaggc tacatttcag gtaaacttct aacacgcttg ggtgaatcag tatgtaatgt
    34921 gcaaagtgat gcacaagtca ctctctcgtt atatgtcgat ccacaacgcc taactgtgat
    34981 taaaggcaca gcaaaagtag atgtggaatt tgattgccaa cgatgtggtg acccgtttac
    35041 acaaacgctc gactgttcat ttgttttcag tccggtgtct aacatggatc aggcggacat
    35101 cttgcccgaa atttatgaac ctatcgaagt aaacgagttc ggtgaagtaa atttactaga
    35161 tatgattgaa gatgaattta ttcttgaatt acctctagtc ccgatgcata gtgaagaaca
    35221 ctgtgaagtg tccgtaagtg aacaggtatt tggcgagttg ccggaagaac tggtacaaaa
    35281 accaaacccg ttcgcagtat tagctaattt aaagaaaaac tagatctagg agaaagcccg
    35341 tggctgttca acaaaataag aaatctcgtt cacgtcgtga tatgcgtcgt tcacacgatg
    35401 cattaacaac tgcagcagtt tcagtagata aaaccagtgg tgaaactcac tttcgccacc
    35461 acgtaactgc agacggttac taccgtggtc gtaaagtaat caacaagtaa ttcctttcga
    35521 ggtttatctt ggttgatatc actcttgcag tagatgtgat gggcggggac tttggtcccc
    35581 gcgttactat tcctgcactg gaaaaagcct taaaacaaaa tccaagcctt tcttttttat
    35641 tatttggtga tgaacaccaa atagcccctt tattacaaaa acacacacta ctcaaccacc
    35701 ccaaaatcca aattcatcat accgaacaag tgattgaagc tgatgttccg ttcactactg
    35761 caatccgcca aagcaaaaac agctctatgc ggttagcgat tgaggcagta aaagagggca
    35821 aagcacaagg ttgtgttagt ggcggtaaca ctggtgcgtt aatgggattg gcaaaattat
    35881 tgattgaacc attaccaaat attgaacgcc ctgcactcac ttcgctaatt cctaccctca
    35941 acggcaaatc cagcgtaatg ttagatttag gggcaaatat tgaagtaagc aatcaccagc
    36001 tacaacaatt tgcagaaatg ggaaacctgt ttgctcaagt aatgcttgat ttagtttacc
    36061 ctcgcctgtc tttattgaat attggtacag aagaaaataa aggcacggca caacttcagg
    36121 cagtgcatca actgctgaaa aacagacaag acttaaatta caccggcttt attgaaagcg
    36181 ataaattaac cagctatctc actgatgtga ttatttgtga cggctacacc ggcaatatcg
    36241 cgttaaaagc gttggaaggg gcagctaaaa atattatttc tctctttaaa aaagagaaag
    36301 ccgattccaa tatttgccgt aacaccaaac gatatttact gaaagcgatg ttctaccgtt
    36361 actaccgcaa attgcaacaa attaaccctg accgccataa cggtgcgacc ttgctcggct
    36421 tatctaaagt agtcgtgaaa agccacggcg gggcaaatat caatgccttt tattacgcta
    36481 ttgattatgc ggtgcaacaa attcaaggcg acattccaaa taaaatccta gagggtttaa
    36541 cccaaaaata agcgaactca actgttcgct tattttcatt tcttgtttca cttaaagata
    36601 caagcggtca aatttgcaaa aaaatttacc cattcaaccg cttgtgttta gccttttaat
    36661 tttgccactc gtaaactaac cgcattgcgg tgtgcatcta gcaattctgc acttgccatt
    36721 cgttccaccg tttcggccaa ggctagaaag ccttgtggtg tcagctcctg caccgtcatt
    36781 cgcttgctaa aatcagctaa acctaagctc gaataggttt tagtgtagcc gtaagtcggt
    36841 aaaacgtggt tagtgccgct agcataatcc cccatacttt ccggcgaata cgcccctaag
    36901 aaaatcgagc cggcattatc taaatccgcc aacaacgagc gagcattttc agtttgcaca
    36961 attaaatgct cgggtgcata tttattacta atttcgaccg cttgttgcag gttttccgca
    37021 ataaaagtgc gactgtgagc caatgcttta cgggcagttt cggcacgagg taaaaccgcc
    37081 acctgctttt ctaattcttc atcgactgcc cttgccaagc tctcacttgg tgtgactaaa
    37141 atcacttggg aatcggctcc atgttctgct tgagagagta aatcgctcgc cacaaatgca
    37201 gggtcagcaa actcatcggc aatcaccaac acttccgacg ggcctgccgg catatcaatc
    37261 gccgtgcctt cttgcacaac ttggcgtttc gcctccgtca caaacgcatt gcccgggccg
    37321 aaaattttat ccactttcgc taccgtttgt gtgccatttg ccatcgcaat aatcgcttga
    37381 gcccccccaa ctgcgtaaat cgtttccacc ccacacaaat gggcggtgta gagaatttca
    37441 tcggcaatcg gcggtggtga gcaaagcaca atcttgttac aaccggcaat ttttgccggc
    37501 actgcaagca ttaacacggt agagaaaagc ggggctgaac cgcccggaat ataaagcccc
    37561 accgcattaa tcgggcgagt gatgacttgg caacacaccc ccggctgggt ttcaatatcc
    37621 acctcttgat tttgctgtgc cttgtggaaa gcctcgatat tcgctttcgc attttgaatc
    37681 gcttgcttaa gctctggtga aatacggctc tgtgcttgct gaatctgctg ttctgataca
    37741 actaagctct ctaattttac tttatcgaac ttttcagcta attcaaataa tgccttatcg
    37801 ccgttggcaa ttacattcgc tttaatttca ttaactgccg ctttaatact ctcgccaacc
    37861 acctgtgccg gacgggcaag atattgctgc tgctcagttt ctgttaaatt gttccaaatt
    37921 agggtttgca tatttattcc ttttacgcca acattttctc aatcggtaaa accaatacgg
    37981 aacttgcgcc catttctttt agctgctcca ttgtttccca gaacaggttt tcttggctga
    38041 caacgtgcat tgccacacgg gtttggtcgt tagctaacgg taaaatagtc gggttttcca
    38101 caccgggtaa taaagcggta atttccgcca gtttgtcttt gggggcgtgc agcataatat
    38161 acttagattc gttcgcctgt tgcacacctt gaatgcgggt taataattta tccactaacg
    38221 cttgtttttc cgctgaaagt ggttcttggc gttgaattag gcaagcagtt gaacggtaaa
    38281 tcacttcaac ctcttttaat ccgttcgcct ctaatgttgc acctgaagaa accaagtcac
    38341 aaatcgcatt cgcaattccg gcacttggag caacttcaac cgaaccggtg agtaaaatac
    38401 ttttaaacgg cacattttgc tctttcatat accgctttaa taaattgggg taagaagtgg
    38461 caatcacagc atcttctaaa tctttgatat tgttataatt tttatcacga tcaatagcta
    38521 acgataaacg gcagccacca aaatctaact gacgaagttt tttataggca acattctcac
    38581 cagcacttaa tcgccctaat tccacttcct ctaatacatt ttcaccgata atcccaagat
    38641 caactacgcc gtcaaaaact aagcctggaa tgtcgtcatc acgcacccgt aaaatttcaa
    38701 tcggcatatt ttctgaatag gcaatcaggc gttgatcgtt ccaaatgatt ttcacgccac
    38761 attgtttaag caactcacta cattcttggc ttaagcgacc ttttttctgc atcgcaattc
    38821 ttaatcgatt ttgtgttgtc attgttgttt ccttcttatg ttttaa
//
LOCUS       DANLUA010000005        32660 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLUA010000005 DANLUA010000000
VERSION     DANLUA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32660
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..726
                     /locus_tag="PXB44_000235"
     CDS             <1..726
                     /locus_tag="PXB44_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5280759.1"
                     /translation="LVVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTI
                     DFDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEA
                     VDIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINL
                     LEYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDS
                     IMSILRFYMMETARRLSARFANV"
     gene            complement(817..1920)
                     /locus_tag="PXB44_000236"
     CDS             complement(817..1920)
                     /locus_tag="PXB44_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5280760.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(2107..3114)
                     /gene="lipA"
                     /locus_tag="PXB44_000237"
     CDS             complement(2107..3114)
                     /gene="lipA"
                     /locus_tag="PXB44_000237"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL5280761.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            complement(3129..3782)
                     /gene="lipB"
                     /locus_tag="PXB44_000238"
     CDS             complement(3129..3782)
                     /gene="lipB"
                     /locus_tag="PXB44_000238"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL5280762.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            complement(3844..4143)
                     /locus_tag="PXB44_000239"
     CDS             complement(3844..4143)
                     /locus_tag="PXB44_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL5280763.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            complement(4311..5489)
                     /locus_tag="PXB44_000240"
     CDS             complement(4311..5489)
                     /locus_tag="PXB44_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL5280764.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            complement(5503..6162)
                     /locus_tag="PXB44_000241"
     CDS             complement(5503..6162)
                     /locus_tag="PXB44_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL5280765.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            complement(6254..7381)
                     /gene="rodA"
                     /locus_tag="PXB44_000242"
     CDS             complement(6254..7381)
                     /gene="rodA"
                     /locus_tag="PXB44_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL5280766.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            complement(7374..9323)
                     /gene="mrdA"
                     /locus_tag="PXB44_000243"
     CDS             complement(7374..9323)
                     /gene="mrdA"
                     /locus_tag="PXB44_000243"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL5280767.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            complement(9347..9814)
                     /gene="rlmH"
                     /locus_tag="PXB44_000244"
     CDS             complement(9347..9814)
                     /gene="rlmH"
                     /locus_tag="PXB44_000244"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL5280768.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            complement(9891..10205)
                     /gene="rsfS"
                     /locus_tag="PXB44_000245"
     CDS             complement(9891..10205)
                     /gene="rsfS"
                     /locus_tag="PXB44_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL5280769.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            complement(10373..11242)
                     /locus_tag="PXB44_000246"
     CDS             complement(10373..11242)
                     /locus_tag="PXB44_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5280770.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            complement(11293..12477)
                     /gene="uxuA"
                     /locus_tag="PXB44_000247"
     CDS             complement(11293..12477)
                     /gene="uxuA"
                     /locus_tag="PXB44_000247"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL5280771.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            complement(12507..13268)
                     /locus_tag="PXB44_000248"
     CDS             complement(12507..13268)
                     /locus_tag="PXB44_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL5280772.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            13432..14457
                     /locus_tag="PXB44_000249"
     CDS             13432..14457
                     /locus_tag="PXB44_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5280773.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            14486..15469
                     /locus_tag="PXB44_000250"
     CDS             14486..15469
                     /locus_tag="PXB44_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5280774.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            15530..16009
                     /locus_tag="PXB44_000251"
     CDS             15530..16009
                     /locus_tag="PXB44_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5280775.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            16012..17283
                     /locus_tag="PXB44_000252"
     CDS             16012..17283
                     /locus_tag="PXB44_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL5280776.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            17295..18140
                     /locus_tag="PXB44_000253"
     CDS             17295..18140
                     /locus_tag="PXB44_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5280777.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENTKSFFELDKAGIEFVFNLNYLGTLLPTQVFAKDMLGKEGANII
                     NISSMNAFTPLTKIPAYSGAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKAL
                     LFNADGTPTARANKILTNTPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFS
                     AYSGV"
     gene            18157..19104
                     /locus_tag="PXB44_000254"
     CDS             18157..19104
                     /locus_tag="PXB44_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL5280778.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            19192..19830
                     /locus_tag="PXB44_000255"
     CDS             19192..19830
                     /locus_tag="PXB44_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL5280779.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            20054..21508
                     /locus_tag="PXB44_000256"
     CDS             20054..21508
                     /locus_tag="PXB44_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5280780.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(21556..24153)
                     /gene="mutS"
                     /locus_tag="PXB44_000257"
     CDS             complement(21556..24153)
                     /gene="mutS"
                     /locus_tag="PXB44_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL5280781.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            24303..24866
                     /locus_tag="PXB44_000258"
     CDS             24303..24866
                     /locus_tag="PXB44_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL5280782.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            25040..25669
                     /locus_tag="PXB44_000259"
     CDS             25040..25669
                     /locus_tag="PXB44_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL5280783.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            25817..26446
                     /locus_tag="PXB44_000260"
     CDS             25817..26446
                     /locus_tag="PXB44_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5280784.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            26512..27027
                     /locus_tag="PXB44_000261"
     CDS             26512..27027
                     /locus_tag="PXB44_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL5280785.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(27075..28005)
                     /locus_tag="PXB44_000262"
                     /pseudo
     CDS             complement(27075..28005)
                     /locus_tag="PXB44_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(28064..28714)
                     /locus_tag="PXB44_000263"
     CDS             complement(28064..28714)
                     /locus_tag="PXB44_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL5280786.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            29043..30401
                     /gene="mnmE"
                     /locus_tag="PXB44_000264"
     CDS             29043..30401
                     /gene="mnmE"
                     /locus_tag="PXB44_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL5280787.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            30402..31667
                     /gene="tilS"
                     /locus_tag="PXB44_000265"
     CDS             30402..31667
                     /gene="tilS"
                     /locus_tag="PXB44_000265"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL5280788.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(31657..32550)
                     /gene="xerD"
                     /locus_tag="PXB44_000266"
     CDS             complement(31657..32550)
                     /gene="xerD"
                     /locus_tag="PXB44_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL5280789.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         9654 a         7269 c         6536 g         9201 t
ORIGIN      
        1 ttagtggtgg ctgataaaat caatctacat tttgaacaag gcaagatcta tactttactt
       61 ggcccaaatg gtgcgggtaa atcttctctc cttaaaactc tatttgggga aattgctcat
      121 caaggcacaa ttgattttga cggtaataaa ttaacctcaa gccacttgca tagttggcgt
      181 aaagaaattg gctatatgcc acaagacaca caggtagagg cctcactcag tgccttagag
      241 gttattttat tagggcaact tgatagcctg aatatgtata ttagcgatga attactcaat
      301 gaagccgttg atattatgga taaactcggt atttcacatc tagctcatca agatattatg
      361 cggctaagtg gcgggcaacg gcaaatggta atgttcgcac aagtattgct acgtaaacca
      421 cacattctgc tgctagatga gcctgtcagt gcgttggata tgtatcatca aatcaactta
      481 cttgaatatg tgaatgccta cactcgagcg aataacctaa ttacaattat ggtgctgcac
      541 gatttaagcc ttgcggctca attttcggat agtgtgatgc tattaagcga ggggaaattc
      601 aagcacaagg cagagcttgt gatatgttgg aatgcgatat tattcagaga ctctataatg
      661 tcaatattga gattttatat gatggaaacg gctcgccgat tatccgcccg cttcgcaaat
      721 gtttaaatat tgattgctgt ttgaaccgca aatagtaaaa gcgaatacaa gcggtcattt
      781 tgcaaaataa atctgcaaaa tgaccgcttg ttatttttat ttcagttgca taaagaatgt
      841 gccttttggc gtaattggta agtagttgtt gtggaaatcc aaatacgttt tttcaggatc
      901 gatatcttta aacaattcag gataaagtgc ttttgccata aattgagcac tcgctaaatc
      961 tgaaattgag cgagaagctg tatgatataa accataaaca cgccctgatt ttaccgcagg
     1021 taactctttc caacctgtac gctcaataaa gccttttaag cgtttttgag catcggcttc
     1081 ttcaatatta atccccatcg acataatctc agggttagtg tcgttgccta gttccgtacc
     1141 tgaaatcata atcacatcag gtttagaaac taacacctgc tcagggttaa tctgccccca
     1201 gttttctaca aacggggcac taatgttatc gcctcgcacc gtttcggcaa tcgctcccca
     1261 catattttta ccaaaggtaa aactatgctc tttcgggcct ttattaccaa attcagcata
     1321 gattttcggc ttacttactt ttgcattatc aatacagctc tgaatatcag caatgccctc
     1381 ttcatactct ttggcaattt tctccgctct ctcttcagtg cctgtaagct gaccgaaaac
     1441 tctcacgctt tttgtatggt tttcaatagt ttgcttgttg aaatctacta caacaggaat
     1501 gccagcttgc tcaatgcgtg aaatttcact ggctaacgct tgatattgaa tttcaggtag
     1561 cactaacaca tctggtttta atgcgatggt tttttctgtt gaaaaagtac cactattcac
     1621 atagccaaga tcggcaatgt tttttagatt tggcattttt tctgcaaata atgcccaact
     1681 gcctgggttg aacttttccc aaaactctcg tgaaatccct actaccttat cgaaattttc
     1741 aacaccggtt accgcaatat agtcagggta atagaaagtc agtgcaacac gtttagcagg
     1801 cacatcaact ttgacctcac gccctaatac atcagtgatg gttttaattt cggcattggc
     1861 agttgcacca aaagcaagca atgtggcagc aaatacgctg tttaatagta attttttcat
     1921 tggaactcca aaaagaaatg gaaagttgaa ttgtaacttt ctataacaaa tagtcaatga
     1981 aaataattcg caacaacgaa attatctata gaagagtaaa ttagaagaga ttacaagcgg
     2041 tttaattttg ctaaaacttt gcaaaccaca aaaacaaagg gcaaataata ttcgccctta
     2101 tcgattttat ttcaccaatt cgcctttcgc ttgtaaatcc gcgtggtaag aagaacgcac
     2161 gaacggtccg caggctgcgt gttcaaagcc cattttttca gcttcctcac ggaacatatc
     2221 aaactctgcc ggtggcacat aacgttccac ttttaagtgg tggcggcttg gttgtaagta
     2281 ttgcccgatg gtgagcatcg tcacgccgtg gtctcgtaaa tcttgcatta cttccaagat
     2341 ttcttcgttg gtttcaccta agcctaccat taagccggat ttggtcggaa tatgcgggaa
     2401 ctcttgcttg aagattttca ataattccaa cgaccatttg taatccgctc ccggacgaac
     2461 ttcacgatag agacgtggca cattttctag gttgtggtta aagacatcag gtggattttc
     2521 tttgagaatt gccaccgctt gctccacacg tccacggaaa tcaggcacta aaatttcaac
     2581 cttacaatgc ggatttaagg ctttgatttc acgcacggtc gcagagaaat gagccgcacc
     2641 acggtcgggc aaatcatcac gatccactga ggtaatcacc acatatttga gtttcatatc
     2701 ttgcaccgtt tctgccactt tgcgaggttc gtccgggtca agcggaagcg gtttgccgtg
     2761 tgccacatca cagaacgggc aacgacgggt acagattgcc cccataatca taaaggttgc
     2821 agtaccgtgg ttaaaacatt cgtgtaaatt cgggcaagag gcttcctcgc acaccgagtg
     2881 caagccgtgg cgacgcatac cgtttttaat gctatcaatt tttgccgaat ttgccggcag
     2941 tttgattttc atccactccg gtttttttaa gagttcttga tccgggtcga tattacgcac
     3001 ctgaatcaca gaggttttgg cggcatcacg atatttcacc cctttttcca ttttgaaagg
     3061 gctcattttt ttcactgtag cagttgccgt cgtttgagct gtcgatgtag ccattactgt
     3121 gttttatcct aattgttaat aatttgttgg ttattatagc ctaaaattgt gctgaaatga
     3181 gccactaatt ttggagcaac ctcttcgcaa cgcaaatcag ggcgatgaac cagttcactt
     3241 aattggcaca tttccagccc tgcataaccg cacggattga tgttttgaaa aggcgttaag
     3301 tccatattga tatttaatgc caaaccgtgg aaagagcaac cttttcgaat tcgtaaacca
     3361 agtgagcaga ttttcttgcc gtccacatac accccgggtg catcggcttt tggataactt
     3421 tctatgccat attctgccaa cgtttgtacc acggtttgct ccagggcagt cactaaatct
     3481 cgaaccgaca cttctttacc caaggatttc agccgtttga tatcaatcag cacatacata
     3541 atttgctgcc cctcgccgtg gtaagtaatt tgaccgccac ggtcgctttg caccaccgga
     3601 atattggttg gatttaacaa atgctccggt ttgcctgccg atccttgcgt aaacacagcc
     3661 gggtgctgaa ccagccagat ctcatcatcg gtattcccat cacggctatc tgtgaaagat
     3721 tgcatttggt gccaaatttc ttcataaggc tgaatgccta gttggcgaat tattaatttc
     3781 attttctatt ttccaacatc gctttattga aaaattgagg gcgaatgtca ttcgcccctc
     3841 gcattataat accattctta cgccattaat tttggcaagt tccgtgtaga gagtttctac
     3901 ttgctcaata ttttccgcca caatatcaat cgaaacggaa ttataggtac cttttgagct
     3961 tttttgctga cgtggattgt aatcgccttt ggcatatttt tgcgtgacca ctaccacatc
     4021 atcaaccaaa tcttctcggt ttgctcccac cactttgaac gtgaaatggc acggaaactc
     4081 tagcagatct tttaatttgg cttgaggtaa atcttgtaag tttatcgctt tgggttgaga
     4141 catttttgat ccttcttcta ttctttcttc gaggggcgaa aaatttttcg ccactacact
     4201 ttttatacat tcatatctaa aacggtaagc ggtgaaattt tgcaaatttt ttgcaaaatc
     4261 acaccgcttg cattgctccc tagatgggga taaaaaggca aataacaaga ttaatcgaac
     4321 aagctcttca cagtaagggc aatccaatcc cacgctttgc cgaaaatacc ggcttccggt
     4381 acatcttgta agacttgtaa gttagtgctt gcaacatctt tgccgtcaag ttggtagatc
     4441 attttaccca ctacctgccc ttttgctaaa ggagcttcga ggtttttctt ctctaactca
     4501 taacgggctt ttaaatctgc cgagcgacct ttcgggatag tcacaaagct atcttgcacc
     4561 gagcctaatt gtactttatt gacttcaccg taatagacac tttgctctgc caccgattga
     4621 gctgccggaa tagatttaat ggtttcaaag ttcgcaaagc cccattgcag taatttctta
     4681 ctttccactt cacgcccttt ataagttggc acgcccatca ctacagaaat taaacgtgta
     4741 ttcgggttaa ttgctgatgc gactaagtta tagccggctt tatcagtatg ccctgttttc
     4801 ataccatcaa cttgaatggt tttatcccac aataatccgt tgcggtttgg ttgtggtttt
     4861 ttgatgttat attggaactc tttttccgca taaattttat actcttccgg ctgatcacga
     4921 ataatatgcg aaccaataat cgccatatca cgagcagaag aatattgctc agggtcatct
     4981 aaaccgtgaa cagtggtaaa gtgagtattc tttaagccaa actgttgggc atatttgttc
     5041 atttcattaa tgaacgctgc ttgtgagcct gaaacatgtt cagcaagcgc cacagaggca
     5101 tcattacccg agacgataat cagccctttc atcaactcac ctactgaaac tttgctgttt
     5161 aaatctaaaa acattaatga agagccgggg aatttttgcg cccacgaatt ttcggtgact
     5221 atcaccatat cactgttttg cacttttccc gattttaggg cttcaccaac tacgtagcta
     5281 gtcatcattt tggtcaatga tgcaggatat tgacgttgat ccggatttag gctcactaaa
     5341 acttcaccgg agttgtaatc catcaaaata taggtttgtg cgttgatctg gggtgccgat
     5401 aaattgaagt tagtgtttaa atccgcatag gcaatgcttg aaaccgcaaa acctaccacg
     5461 ctcatcgctt tcactaatgt ttttttcata ttccctctta aattaattca ttacataaaa
     5521 ctgattagtc gatcccaatt ttgacttatc aacaatcgca attttcactc ttgccgtccc
     5581 cgaatggtgc ataccgattt ttttcgctgc ggctgatgat aaatccaaga cccgtgcgtg
     5641 agcataaggt ccgcgatcat ttacacgcac caccacagaa cgaccatttc gtaaattcgt
     5701 cacttcaatt aaagtaccaa atggtaaagt tcgatgtgca gccgtcattt ttgcgttatt
     5761 gaaagtctcc ccattagcgg ttcggcgacc attaaagtaa tttgcataat agctggcaac
     5821 accagtctgg aaatgttttt tgaccagtgg cttatgctgc ttttttacca attttttctt
     5881 agcggttacc gttttcttga caaccttgcg agccagcttt tttgtagatt taatcggctt
     5941 agttgtaaca gtagaaattg ttttcttttt tgtagcttta gccactactt cttttttctt
     6001 aactttagaa atacctactt tttttattgc tttagaagtt gcttgcttct tggctaaaat
     6061 actcgattta gcttttgtat tctttgtcgc agccaaagaa tttaatgaac taaatgtcag
     6121 aaatccagct aacaacaggg ttacaatttt acctaatctc atataagttt tttcctctat
     6181 gtggatgtcg ttattgtttg taacgatgat cgtttcactg ctttttgata ccgcaaaaaa
     6241 gcaaaagttt aatttatttc agtttatcat aaggattatt actacctaca cgcttacggt
     6301 gaacataggt tgacatcatc aaaccaaatg ccgccatcag cgtcacatag gaggttccgc
     6361 cgtaactgaa aagcggtaat ggtacaccga ctacgggtaa aataccactc accataccaa
     6421 tatttacaaa aacgtaaaca aaaaatagaa gggctgtacc acctgataat atacgcccga
     6481 aagcagaatc cgattttgtg ccaatcacta aacctcgggc aataataaac aaataaatcg
     6541 ctaataaaat cagcacacct atcataccat gctcttcgct taacactgca aaaataaaat
     6601 cggtatgcgg ttcaggtaaa aattcaagct gggattgcgt tccttccatc cagccttttc
     6661 cattaatgcc accagagcct atcgcaattt tggattgaat aatatgatag ccggcaccca
     6721 acgggtcttt attaggatca attaaggtca tcacccgggt tttttgataa tcgtgcatta
     6781 agaaaaacca cattatcggg ataaaaccgg ctaaaaacac cacacctgcg ccaattaatt
     6841 tccaacttag ccctgccaaa aataaaacaa aaataccggc agaacacact aaaatagatg
     6901 ttcccaaatc gggctgaatt gccaccaata aggttggcac aataataatg gctaaggcga
     6961 taaaagtatc tttaaaactc ggaggaagcg gccgcttacc taaataagtg gctaccatca
     7021 gtggcacagc gagcttggca atctcagaag gctgaaagcg aacaaatcct aaattcaacc
     7081 aacgttgcgc tcctttactg gtttcgccga ccaaatccac caaaatcaat aatatcagac
     7141 agaaaatata cagataaggc gaaacttttt gataaaagcg aggaggtacc attgccatta
     7201 tcaacattag cccaagccct agcgaaactt gaatcagtct actggtaaac atccgctcat
     7261 ttgcaccgct tgcgctatat agtactaata aaccataacc ggtaatagct aataatcctg
     7321 ataataaaag gagatcgagt gaaaaaattt tccagaaaag ttttttaaag ccactattca
     7381 tgatgttcct ctgtgacaag cggtttattt tgctcagaat tttgcgaatc cggctgttca
     7441 ccaatgcctt ttctcaaagc ataatccata atctgacgag caacaggagc cgcggcacta
     7501 ccaccaccgc ctgcattttc taaaattaat gccaccacta ttttaggttg ttcataagga
     7561 gcatagccga taaaccaagc gtggtcgtgc aactcttttt taatcgcatt tttatcgtaa
     7621 tttttaccgt ttaagttaaa cacctgagcc gtaccggttt tccctgctga atgataaaat
     7681 gcaccggcaa acacttttcg tgccgtaccg tttgcggcgt ggtttacgtt atacattccc
     7741 tgttttgcta aattccaata gacatccggc acgcctttaa tatcttcata caacaatggg
     7801 tcttgatacg gcgtaaccga attactgctt tgcacttcaa gcattaaatg cggtgtattc
     7861 acccgcccgt tgttgattaa caccgccgtg gctttcgcca actgtaacgg ggtcgaaatc
     7921 cagtagccct gcccaatccc aaccggaata gtatcgccct gcacccaagg ttttttatag
     7981 cgtttttgtt tccattcacg ggttggcatt acccctgcgg attcctcaaa aatatcgata
     8041 ccggttttca ctccgaaacc gaattttttc atccaatccg acattttatc aatccccatt
     8101 ttataggcta attgatagaa aaaggtatca gacgattcgg taattgcttt attcacatta
     8161 gttgcaccgt gtccgctacg tttccaatct cgataacgtt gtgtagtgcc gggcaaaatc
     8221 caatagccgg ggtctgaaat ggtggtagaa ggggtaatca ttccctcttc caaacctgcc
     8281 accgccataa atggcttaat agttgaagcc ggtggataag tgccttgtgt agtacggcta
     8341 taaagtggtc gggttggatc gtccaataat gctttataat cctgccctga aataccgccc
     8401 acaaataaat tattgtcata acttgggttt gagaccatcg ctaaaatact gctatcacga
     8461 gggtcaagca ccaccaccgc ccctttatgc ttagcaatta acgacatcac atattgctgt
     8521 aattccacat caatactcaa gcgaatactg ctaccggcaa cccctgcttg gtcacgcaat
     8581 ttacggatta ctttccctcg gttattaatt tctacctctt caaaaccggt gacaccgtgg
     8641 agctgatctt catagaattt ttcaatcccc aatttcccca tatcgtgcga accggcataa
     8701 ttgccgaatt tcccggcttc ttgcagcttt ttcttatcgt tttcgttaat tttcgccaca
     8761 taaccgataa tatgggttaa aagctcgccg taaggataat tccgcttata atacgcctgc
     8821 acatctaaac ttggaaaacg gtattggttc accgcaaaac gagccatctg agcctcattc
     8881 aaattgcctt tgagcataat tggggtatag cgagaactgc gttttttctc tttatgataa
     8941 ttctcgatat cttcgtccgt cagcccaaca atatccttca actcggttaa agtctgctca
     9001 atgttttccg tcttttcagg cacaataaac agcccgaaat aggttaaatt ttccgccaat
     9061 actttaccgt tacggtcata aattaagcct cgagccggtg gcacaggcaa caatttaatg
     9121 cggttaccgt ttgaacgggt ttggtagtcc tcataattca ccacttgcaa gtgatacaag
     9181 ttggttaata aaacaccact taacgccaac acccccagaa acgcaactaa ggcacggcgg
     9241 gcatacagat tgctttctgc ctgcccgtct cgcacctttt ctcgctggtt tgaagctgtg
     9301 aattttgcta atttaccaaa catataacac tatctaaaat gactcactac tctcgatgat
     9361 aagggtgatt aatggtaacc gaccacgccc gatacaaact ttccgccacc accacccgca
     9421 ccagtgggtg aggtaaggtt aaaggcgaca acgaccaact ctgctcggca gcggctttgc
     9481 attccggtga taatccttcc gggccgccaa tcaataacgc tacatctcgc ccatcgtttt
     9541 tccagccctc taactgagtg gctaactgct cggttgtcca cgctttgccc ggaatatcca
     9601 aagtcacgat ttttgccttg cccgctgccg ccaacattgc tttgccttca agctcaagaa
     9661 tgcgtttagt atcagcattc ttgccccgtt ttcctgccgg aatttccacc aactcaaacg
     9721 gcatctcttt cggaaaacgg cgttgatact cttcaaaccc tgtcgttacc cacgccggca
     9781 ttttagtgcc aaccgcaatt aattgaattt tcatctctct ttcacttaca agcggtttaa
     9841 tttgcaaaaa atgttgcaaa aatcaccgct tgtcgttcct tattgaactg ctacgcccaa
     9901 agtttctcta attggtacat ctcacggcta tcggattgca acaagtgaac cattgccttc
     9961 ccaaaatcga ccacgatcca atcggcaact gctttacctt catctccaaa tacctcgaac
    10021 ccagcctgtt ttgcttccgt aattaaacgc tctgccgttg aagcaacgtg acggctcgat
    10081 gtaccggtgg ctaaaatcat cgtatcagta atgctagatt tccctttcac atcaatggct
    10141 aaaatatctt gtgcctttaa atcatctaag gttttggtta aaaattcgac taaattctgt
    10201 tccacgtttc tctctctcat cttataaaaa tgtagaattt taacatagat tgaacggata
    10261 aaagaaagaa agcggtcatt tttgcaagat tttttgcaaa aatgaccgct tgttacttac
    10321 tctaaaatgc ctaactgctt ccaatactat ggcatatttg cttttgtttt acctattttt
    10381 tgagtcgtag aatagggagt cgataaataa tcaatgctat caaaatcaag ctgcaaccta
    10441 gccattttgt cgcactaagt tgttccccca ataacatcac gctcaataac aatgtccaaa
    10501 ccggctcaag taacataatg agtgcagaag taccaatatg gcaatatttt tgccccagtg
    10561 tttgcattaa aaaacgtaaa tttgtcgcaa tcaatacact tgcggcaaac cagagccaca
    10621 cttcggtgag aatcgggaaa tgccaacttt ccataaaata ggaatacaag ctacagaccg
    10681 caccgactat cgccaattgg atagtaatta atggaaacaa tgagacttgt ttggcgtatt
    10741 gattatttaa cacaaaatat aacgccccaa acactgatga caccagaaaa atcgtactgc
    10801 ctaaggaaaa atgaaagatg cctttctccg cagaaagaaa gtaaagccca cttaatgcaa
    10861 tgggtaaggc taaccaaaaa atcttcggtg gcgtatgttt gaatatcaac caagagagca
    10921 aaggagcaat cagcatcgca aggctcacca aaaaagcccc ttcaccaaac actgtactaa
    10981 aagccagccc caatacccaa aaaatagtat aaattccgta agtaagaccg gtaatcaccg
    11041 aatatttaac ttgaacctta tctaaacgat acagctctcg gtaagaaaaa ggcagaagaa
    11101 tcaaagaggc taatataaaa cgccctccca taaagccaat aggcggtaat ccaactaaag
    11161 cgtgtttaga gaaaaaccac cccgctgcag caataaaggt aacgagaaaa aggattattt
    11221 cgccgaaata ttttttgaac atatcaaact ataaaatgcc ctctacagta gagggcatta
    11281 taataaataa ggctaatcgt taaaatagac tttcttaagt gccaactcca atccacggaa
    11341 ctccgctaat cctcttaaac gaccaatcgc agaatagcct gggttggttt ttttcttcag
    11401 atcatcgagc atttgatggc cgtgatccgg acgcattgga attaatcggt gatcgccatt
    11461 tgccttgcgg cgatactctt cggtaagtaa tgctttcacc acgccaaaca tatcgacatc
    11521 accggcaaga tgatccgctt cgtggaaact ttgtggattt tcttcacgtt gggtagctcg
    11581 taagtgagca aaataaatgc gatctgcaaa ctgctctgcc attttgacca aatcattctc
    11641 ggctaatacg ccgtatgaac cggtacagaa cgtgaagcca ttggctgcaa gcggttgagt
    11701 ttccacaaac cattgcatat cttctatggt tgaaacaatg cgtggcaagc ctaaaatcgg
    11761 gcgaggcgga tcgtcagggt gaatcgccat acgaatgccc acctcttgtg caaccggcac
    11821 aatctcattt aagaaatagg ctaaatgggt acggaatttt tcagccgaga gcccttgata
    11881 gcggtcaagc tgtgcttgga attcttcgag cgtatagcct tcctcagacc ccggcaaacc
    11941 ggcaatgata tttttggtta attttgcaat atcagcgtca ctcatctgct caaaataggc
    12001 tttggcttcg gcttgttctg ctgatgagta agtggcttcc gctcccgggc gttttaagat
    12061 atataactca aacgccgcaa acgccacttg gtcaaaacgc aatgcttttg agccatcagg
    12121 catttcatac tccagatcgg tacgagtcca atctagcacc ggcataaagt tatagcacac
    12181 cgtatcaatc ccacagcttg ccaaattacg caaggtttgc ttatagttat caatccattg
    12241 ctgatagttg cctgtttgag ttttgatttc ttcgtgaaca ggcacacttt ctaccaccga
    12301 ccaaaccagc cctttttcag ctaataatgc cttacgttta ttaatttcct caaccgacca
    12361 cacctgcccg ttgggaatat ggtgcaacgc tgtcacaatc cccgttgccc ctgcttgtct
    12421 tacatcatct aaagataccg gatcgtttgg tccataccaa cgccatgctt gttccatagt
    12481 aaactcctca ttgtgtagtt taggttttag atacctacta caacggggtt tacattgaat
    12541 agatatccat caaatttagg atcctctaca tcagacaatt caagcaattt agttttaaca
    12601 ttttctagat gttgccacat tgctttacga gctaatgcag gattttttct ctgtattgca
    12661 tttaaaattc gctcatgatc ctctatccac aagtggtgat gatcatgctg taaaattctg
    12721 gagtgtagct gagcccacat cgcactatta aagcggtatc gccaaagctg ttcttgtaat
    12781 tgaatcagaa catcgttacc cgtcatatga gctaatgcaa gatgaaattc cttatctgca
    12841 ctgtagtcat ccatcttctc taacaaaagt ttcttctcac tttctaatat gttacgaagt
    12901 acttgtatat cttgtttggt agcctgctgt gctgcaaact cagcaatact gctttctaat
    12961 aattgtcgtg cttgaagtag ttcaaatggc ccaacatccc ttagctcatt atctttctct
    13021 tctggaaaat cttctggaaa aagctccggg actctaatga catacacgcc cgatcctttt
    13081 tttacctcaa taagcttttc tacttctagc atcactatcg cctcccttac gatagttcta
    13141 ctcacttcaa atctttcaga aatatctctc tcagtaggta acttatctcc tactctgtat
    13201 tttccttgag caatttcctc tttgagaatt tttcctattt tagtgtaagt tctggaacta
    13261 ttttgcataa atcctcacga aattggaatt ccaaaatttc atttttgtga tcaggttcac
    13321 aaaatcacaa aaattggaat gccaaacttg ttttttggtg ttagtatctg tgcataaaat
    13381 accattgaca ctacaaaatg tcgattaact ttatcaaaaa aggacaaaat catggaaaaa
    13441 ccactcaaat cagtatgtct tgtagaacct aataaagttg gtatacaaac cgtttcttat
    13501 cctgttaaag gtgaaaatga tgtgctaatc aaagtagaag gtatggggat atgtggctct
    13561 gatattggag catttagagg cactaatcca cttgttactt atcctagaat tttagggcat
    13621 gaaatcgttg gcacagtaat tgagtctggt aaaggtatgc caaataatat taatgtagga
    13681 gatcgtgtca tcattgaacc ttacatctat tgtggacatt gctatccctg ctccattggt
    13741 agaacaaatt gttgcgaatc tctaaaagta ttgggggtac atattgatgg ggcaatgcaa
    13801 gaggttgtta gacatccagc ccatatgata atcaaaacac ctgatatgcc aatacacgaa
    13861 ttagcgttag cagaaccctt aactatttcc ttacacgcaa ttcatagaac aaaagtcaaa
    13921 gctggtgaac atgttgctat cattggagca ggggcaattg ggctaatggc tgcattggtt
    13981 gccaaagctt atggagcgac gccgatttta attgacattt tagataagcg attggattac
    14041 gcaaaatcta ttggtattcc acacacaatt aatccaacaa aagaaaacga tcttgaagct
    14101 attaaatcaa ttaccaatgg aagaatggca gaggtggtta ttgaagcgtc aggagcaaat
    14161 gtcgcagtac aaaatacgct taaatacacc tcttttgctg gacgtattgc tttaaccgga
    14221 tggccgaaaa cagaaacacc attaccaact aatttaatca ccttcaaaga acttaatatt
    14281 tatggggcaa gaacaagcaa gggggcattt gaagaagcat tagagcttct ggcatcaaga
    14341 agaattgaac caaaagatat cattagtaag gtaattacat ttgatgaaat acctcactat
    14401 atcgaagaac tctcagctaa tcctgatgat tacttaaaaa tcatcgctgt attttaaata
    14461 ctaactcacc tacgaggtaa taactatgaa actcaaaact ttcactaaag ctctattaat
    14521 ttctagcctg atgttcaccg gttcggcatt tgcgaagact atcattaaga ttggtcacta
    14581 taattcagat gttcatccat caaatatcgc attgcaggaa tacttcaaaa aaaccattga
    14641 agaaaaaaca gaaggacgct acgaaatccg tattttccca aataaccagt tgggaggaga
    14701 agatcaaatt gtaaatggtt tgcgtaatgg cacgattgaa ggtggcatta caggtttatt
    14761 actacaaaat gttgatccaa tttttggcgt atgggagtgg ccatatctat ttaaagataa
    14821 tgccgaagct aagaaagttc tagaatctcc tattgctcaa gaaatcgggg aaaaaatgga
    14881 gaagtacggc attaaattac tagcgtatgg tatgaatggc ttccgtgtta tctcttccaa
    14941 taaaaaagtt gagaattttg atggatttaa aggtttgcga ttaagggttc cactaaattc
    15001 gttgtttgtg gactgggcaa aagcaatgca tattaaccct caaagtatgc ctttaagtga
    15061 agtatttacc gcactagaac aaaaagtcat tgatgggcaa gaaaatccat atatgttaat
    15121 taaagattct ggtttatacg aggtgcaaaa atatattatt gaatctaatc atattttctc
    15181 acctggctta ctacaactta gtttgaaaac ttggaataaa atgtctaaag aagatcagac
    15241 aatcttctta gaggcagcaa aactctacca agaaaaagag tgggaattag cgatgaagat
    15301 ggaacaagat gtaaaagatt tcttccacaa aaatggcaaa gaagtcattg ttccgagtga
    15361 acaatttaaa gctgatatgt taaaagcatc cgaaacgctt tatcaaaatt tctatcagaa
    15421 atatgattgg gctaaagatg ttatttcaaa aatccacgca gccaaataag caataagcga
    15481 ggcgaccgga taaaagaggt cgcccatcta aatgaagaag gagaaattta tgcaaatttc
    15541 tacagcatta gatagaactc tcaaactcct tgcgataatt gctctatcta caatgattat
    15601 cctagtcttt tttaatgcgg ttttacgata tttttttgac tccggaatag cttggtctga
    15661 ggaattcgcc agaatctgct ttgtctatat gatctttcta ggaataatcc tcgttgcaaa
    15721 ggaaagggga cacctcaccg ttgatattgt tgtttctgca ctaccggcaa aagtacaaat
    15781 gtacttactg ttctttgctg atattttagt cttagttgca ctgtcattta ttacttacgg
    15841 tgcataccag ttaatgttat tgacatacac acaaaaaatg ccagcaactg aaatttcatc
    15901 tgcgtttcta tattttgcag cagttatttc atctatctcc tatttcgcta ttgtattcac
    15961 aaatttatgg aaacatattc ggctagcttt cattaaagag gagaaataaa tatgttatgg
    16021 ctatttttag gaagtctatt cctctttctc tttttaggag tgcccgttgc tctctctatg
    16081 ctacttagtg ccagttttat gctatggaaa atggatatgt ttgatgccca attaatcacg
    16141 gagaatttcg taatgggtac aaataatttt ccgctgatgg ctatcccgtt ttttatgctg
    16201 accggtgaaa ttatgaaaca cggcggtatt tccgaacgta ttatcaattt tgcaatgagc
    16261 atggtcggcc atattaaagg cggattgggc tatgtagtga ttatttcagg cttaattttc
    16321 gcaggtttat ccggctccgc tgttgctgat accgcggctc tcggtgcaat tcttattccg
    16381 atgatggttg caagaaaata tgatgttgca cgttcaacag gcttaatctg tgcagcaggt
    16441 attatttctg tagttatccc accaagcatt ccaatgattg tctatggtat tacagccggt
    16501 gcatcaatta ccaaattatt tatgggagga atggttccag gctttttaat ggttttaggt
    16561 ttatggttag tttggaaatt cttataccga aacgaagaac aaaccgttga accgaaaaaa
    16621 acaggtaaag aacgttgggc tgcatttaaa tcagcattct taccattgct acttccaatt
    16681 attattatcg taggtttaag aggcggcatc tttacaccaa cagaagcggg agttgtagca
    16741 gcaatttatg cagcaataat cagcattatc ttaaaagggt taaccttaga taaaatccaa
    16801 gatgttttct taggcactat caaaacaact gcaatggtta tgtttgttgc agcggcggca
    16861 atgatatctg catttgcagt tactgttgct caaataccta tggagttagt tgagtttatc
    16921 aaaggtatta ccgatagccc gactgtacta atgttcgtca ttatgatttt cttattatta
    16981 gtcggctgtg taatggactt aatcccagca gtattaattt ttgtaccagt actattacca
    17041 ttactaagag cgtataacat cgatattgct tactttggta ttatgatggt aattaaccta
    17101 tctatcggtt taattacgcc tcctgttgga acggtattat atgttggttc tggtatagca
    17161 aaaattggta ttgctagatt aaccaaaggt atctatccat tcttgctagt ctatgctgca
    17221 atactgttct tattaatact tttccctgaa attattatcg taccaatgaa ttggttatct
    17281 taggagttct tattatgtct attgcccaaa atcataattt taaagataaa gttgttgtta
    17341 ttaccggagc aggtggtgtg ctatgtgctt attttgcaaa agaaattgca aaaacaggtg
    17401 caaaagttgc actactcgat atcaatctag aagcagcaca gaattttgct tctgaaattg
    17461 ccgatgaagg ctatattgcg aaagcgtacc aaactaatgt acttgaatta gaaagcataa
    17521 aacaggcaag agatgaaatt ctaaaagatt ttggcacttg tgatatttta attaatggtg
    17581 caggtgggaa taatccaaaa gcaacgacag ataatgaatt tcacgatatt aacttaccgg
    17641 aaaatacaaa atcttttttt gaattagata aagcagggat tgaatttgta tttaatttaa
    17701 attacttagg cacactatta ccaacacagg ttttcgcaaa agatatgttg ggtaaagagg
    17761 gcgctaatat cattaatata tccagtatga atgcctttac tccattaact aaaatccctg
    17821 cctattctgg agcaaaatct gcaatcagta actttacaca atggttagca gtttatttct
    17881 caaaagtagg tatccgctgt aatgctattg caccgggatt tttagtgagt aatcaaaata
    17941 aagctctact atttaacgcc gatggtacgc caacagctag agctaataaa atcctcacga
    18001 atacccctat gggacgtttc ggacaagccg atgaattggt aggtgcaatc ctatttttaa
    18061 tggatcacca ttattccagc tttatcaatg gcgttgtatt acctgttgat ggcggattct
    18121 ctgcttatag cggcgtgtaa cttagaaggt attcctatga aaaaactcgc cattctcggt
    18181 gaatgtatga ttgagttaaa cggggagccg tttggggaga tgcaccaaac ctatggtggg
    18241 gattcgctca acaccgctac ctatttggct cgggtgagtt cgcctgaaca gattgaggtg
    18301 cgttatattt ccgctctcgg cacggataag ctcagtcagg gaatgttagc ggcgtggcag
    18361 gtggataata tcaacacgga ttttgtgttg cgtgacgagc accgccagcc ggggctgtat
    18421 ctgattcagt tggataaaca gggcgagcga acgttcctct attggcgaaa tcaatctgcc
    18481 gcccgttatt tgttgcaaca ccccggctat cccaaagcac tctcggcact aaacgaggta
    18541 gatatggttt acttgagcgg tatcagcctt gccatcttgc cggaaaacga ccgcactttg
    18601 ctgattgcac agttgcgtga gctcaaaaca caaggggtga aaatcgcctt tgacagcaat
    18661 taccggccga aactgtggga aagtgtggct gctgcccaag ctcactatgc cgagctgttg
    18721 caattagtcg atttagctct cgtgaccttt gatgacgagc aactgctttg gggcgatgtg
    18781 aatgagcaag ccacctttga ccgtctcact caatatggca ttcagaccat tgtggtgaaa
    18841 caaggctctc tgggggcaac ggtgttgcaa aacggggagc gtacttttgt gccaactgtt
    18901 gcggtagaga aggtggtcga taccacttcc gccggcgatt cctttaatgc cggcttcctt
    18961 aacggttact tattgggtaa agatgtcgtt acctgttgcc aacaaggaaa tgccgttgcc
    19021 ggtatcgtca ttcagcacaa aggggcgatt attgccaaat ccgccactac tcatttgaaa
    19081 tcgcagtttg aacaatccat ttagaccaac aatagcacaa cgacaagcgg ttaaatttgc
    19141 aaaatttttt acaaaagcga ccgcttgcaa tacaacataa tgaggctaga aatgcgttac
    19201 accaccgaac aaatcattga aaaattaaga caactgaaag ttgttcccgt gattgctttg
    19261 gataaagcag aagatatttt accgctggcg aaaacccttt ccgaaaacgg tttgccggtg
    19321 gtggaaatca ctttccgctc ggaagcagct gaagaggcaa ttaaattagt gcaagccaac
    19381 tatccggaag tattgattgc agcaggcaca gtgctgacca aagagcaagt cttaactgcc
    19441 aaaaacgctg gggcggattg cattgtgact ccgggtttta atccgactat cgttaagtat
    19501 tgccaagtgt taggtttgcc tgttacaccg ggtgtgaaca acccgatggc gattgaagcc
    19561 gccctcgaac ttggcataca agcagttaaa tttttccctg ccgaagccag tggcggcgtg
    19621 aaaatgatta aagccctgct cggcccttac ggcaacttac agattatgcc gaccggcggc
    19681 atctcgccac aaaacatcaa cgactacctc gccattccca acgtagtcgc ctgcggcggc
    19741 tcttggtttg tggagaaatc cctgattaag gaacagaagt gggaggagat tgggaaattg
    19801 acgaaggaag tggttgaatt ggtgaaataa ccatttaaag ttccaagcaa tgagctattt
    19861 tctacccaaa atggatctct ttcctcttat ttacaaagca taacaaatat tgcatacttt
    19921 atttagtagc atatagtagg tatatttaaa gctatatatt taacttaatg aaaaatatag
    19981 caaaacaacc tcaaaactct tcactaaagg ctattgaaaa atagcctttt ctattcaaat
    20041 aaaagatagc tttatgaaaa aaatagtctc aacgctttta atccttacct caaacctggc
    20101 atttgcggaa accacaccgg ctcaacttca agaaaaaatg gataaccagc gaatgcagaa
    20161 aaacctacat acattagaaa agcctgaacc tcaagccaaa ccacaagcgg ttaatgcaca
    20221 ggctcaccgg caatcgatct ctatttcaag ccaacagctg cttcaacagc ctgagttgct
    20281 caactcggca atgctaactg cgttggtcac taaccacact gagaatgttc aattcttact
    20341 gcctatttat caacaattac ccgccagtaa acaaaacagc gaagttgtac aatgggcgga
    20401 ggcagttaat gccaaagcaa aacaaaatta ttcagaggca ataaaaggct accgtcagct
    20461 actggcaaaa taccctgaaa atcagccaat ccgtatgcaa ttagcggttg cattgtttga
    20521 aaatcgggaa aatgaagccg cagaggctca attttataag ttacaagcgg acaaattacc
    20581 gcaagaaatt gcaaatgcgg tcaatagcta tttggaggcg attggcaagc aagatcaatg
    20641 gagttttcaa ggcggcttaa cctatttgaa tgacccgaat atcaacaatg ctccaaatgc
    20701 aggcacgacc tatggcaact ggactgcacc gaaaaaagaa tccgcccaag gggtcggctt
    20761 tcactttgag gcggataaaa agtggtcttg gggcaatggt ttcttccacg agtttcgttt
    20821 aaacggaaac ggcaaatatt actggaataa caaaaaatat aacgaatatt cgctcagaga
    20881 aagcctcggc ttgggttatc aaaatgcaaa acaaaaaatc accttgttgc cgttttttga
    20941 gcagatgtgg tatgccggtg gctcaagcca aaccgaaagc accaaacgct actccaacgc
    21001 aggcggtgtg aatgcccgct ggcaatattg gattactcca cagtggcaat ctgcaatcag
    21061 ctacgaatat gcggaacagc gttacactcg ccgccaacat ttagacggca attatcattt
    21121 tattgccccg agcctctatt tttaccctaa cagccggcaa tattggtttg tgggggcgaa
    21181 ttttaaccgc accagccccc gtgatttaga tgatagcttt attcgccgag gtgtttctat
    21241 tggttgggga caagagtgga aaaacgggct ttcaacccaa ttaagtgcca gctacgcccg
    21301 caaacaatac cgagccccaa tgccgatttt ccaaattaca caacgtaaca gtgaatatgg
    21361 tgtgcaagcc agcatctggc acagagcctt gcattttaag ggtattaccc ctcgcttaac
    21421 gtggcaatat aacaaggtaa aaagcaatca tagcttctat agttatgaca aaaaccgtgt
    21481 ctatattgag ttttcacata cgttctaaac aaaaacgccc cttaaacggg gcgatttctt
    21541 agttattttt tcaatttacc ccaacaattt tttcaagcga tacaactcgt ccaaagcctg
    21601 tcttggggta agttcatcag gctgaagggc tttcagggct ttttcaactt cattttctac
    21661 tacaagcggt tgaattgctt catttttttg caaatcaaaa ccatcggaaa acaacaaatc
    21721 cgcctgtgga tgttcgtttg cttgtttggt ttggtgggaa atttcctcca aatgtgccaa
    21781 acgctgcttt gccagttgaa tcacttgctt tggcacaccc gccaaggctg ccaccgccaa
    21841 gccgtaactt ttgctggcgg ccccctcttg caccgagtgc ataaacacaa tactgtcttg
    21901 atgttcacgc gcgtctaaat gcacatttgc cacccctttg agctgattcg gcaagccggt
    21961 aagttcaaaa tagtgggttg caaacaaagt gagtgactgg gttttcttcg ccagccattc
    22021 ggcacacgcc cacgccagtg ataagccgtc ataggtagaa gtgcctcgcc cgatttcatc
    22081 aatcagcaca aggctgtttt cggtggcttg atgcagaata ttcgccattt cggtcatttc
    22141 caccataaag gttgaacgcc ctgaagctaa atcatcgctt gccccaattc gggtgaaaat
    22201 acggtcaatc gggccgattt ccgcactttc tgccggcaca aaactgccga tatacgccat
    22261 caatgtaatg agggcaattt gccgcatata ggtgctttta ccgcccatat tcgggccggt
    22321 cacaatcaat aaatgccgtt gggaatttaa aaacaccgga ttggcaataa acggctcggt
    22381 taaaacctgc tccaccaccg ggtgtctgcc gcctttaata ttcaccattc gcatcgggct
    22441 aaagctcggg caaacatagc ctaaactatc agctcgctcg gcaaggttgg ttaagacatc
    22501 aagttcagac agcatcatcg ccgctaattg caattcgcct aagcgaggca ttaacacatc
    22561 aaacagttca tcataaagct gtttttcaag ggcaagcgat gccccttttg ctttcagcac
    22621 tttgtcttcg taagttttca gctcagggat aatataccgc tccgcatttt tgagcgtttg
    22681 gcggcgaaca tagtgaatcg gggctttgtg tgcttgccct tggctgatct gaatgtaata
    22741 gccgtgaacc gcattaaagc cgattttcag cgaatcaatg ccggtgcttt cacgttctct
    22801 gatctccaaa ttttccagat attgcgttgc cccgtccgaa agttgccgcc attcgtcaag
    22861 ctcggcatta taaccttctg caatcacccc accatcacga atgagttgtg gtgggttttc
    22921 gataatcgcc cgctccagca aatcgtgtaa cgggctgaaa tccgccagtt gagccaaaag
    22981 ctgatcgata ctagcggtca gattttgtga attttttgca atttccggta actgagccaa
    23041 tgcagtgcgt agtcgggtga ggtcacgagg gcgagccgta cgcaacgcca ctcgggcgag
    23101 aatccgctcc atatcgccga cttgttgtaa taacggctgt aaatccgaca ttcgctcata
    23161 ttgtatcaat gctgttatcg ccttttgtcg ggcttgtaat ttaccacaat ctcgaatcgg
    23221 ctgatgaatc caacgcttga gcagacggct ccccattggg gtcacacatt tatctaacac
    23281 cgccgccagc gtgttttccg tgccacccgc taagttttgg gtcagctcta aattgcgacg
    23341 agtagctgca tcgagcaaaa tagtatcgct atgttgtgcc aaatgaattg cgttaatgtg
    23401 cggtaaagcg gtgcgttgcg tttcttgggc ataatgcaag acacagccgg cagcacaaag
    23461 tgccaccacc gctttttcca ccccaaatcc ggcaagtgtt tgcgtgccga attggcgatt
    23521 tagcagctga atcgccgtta ccagctcaaa ttcccacacc ggacggcggc gtaacccttt
    23581 gtaattcgtt aaaattgagg cagcggaaaa atcttccgca tacagaattt ctgccggttg
    23641 tacacgttgt aactcggcgg atagagcctc tttgctagca agttcactga ttaaaaaccg
    23701 cccggaggtc atatctagcg tagcaatcgc aaatgtgcct ttttcttcgt aaactgccgc
    23761 caccaagtta tcttggcgtt cgggtaacaa tgcctcatcg ctaactgttc ccggcgtaac
    23821 aatccgtacc actttgcgtt ctaccggccc ttttgaggtt gctgggtcgc caatctgttc
    23881 acaaattgct accgattccc ccaacgccac caatttcgcc aaatacccct ccactgcgtg
    23941 ataaggcaca cccgccatcg gaatcggctc accggcagat tgccctcgct tggtgagcga
    24001 aatatccaac aatgctgccg ctcgtttcgc atcatcataa aacagctcat aaaaatcccc
    24061 cattcggtaa aacaatagta tgtccggatt ttgagctttg agttgcaaat attgttgcat
    24121 cattggcgta tgggtggaga aatcttgtgt catcagtcta tttattgtga gaaatcattg
    24181 ctgatagttt aacagattca gccaagggat aaaaacaagc ggtcattttt cgctgaaaat
    24241 ttgcaatggt cagccgctgt ttttttgtta caatgcacca aatttttaaa acagaggtca
    24301 gaatgttaag aaaaatagtg ctactttcta caggcttatt attggcggct tgtagcagca
    24361 atgaaccgca acaaccggaa gcattcccgg gcgaatttgc caatgccgat tatgtgcttt
    24421 cggaagaaaa tgctcaaaaa tgggtaattg ccagcgaaca ggcaaaacag tgtatctacc
    24481 ccaatttaac ccgtattcag caagagcatt tttccaaaga agattcttat attttctcac
    24541 aatatgtatt tttctatccg cttgaagaga ttatcgggga gcagtatgta aaaatcattc
    24601 aaaatgatga aaaatcaatg ggttacgctc aatatttatt caaaaaactg aaaaataacg
    24661 aagcggttga agcgttaccg aaaaaacaat gtgcgacctt acgcaccaat gcaaaaaatg
    24721 atttggcagt ggtaaaaggg cagtatcaaa gcggtatgat cgaagaaacc aatagccaag
    24781 aagggaaatc aggcgataaa gttgccacca atgataataa attcttcttc gatattatca
    24841 aatggggcag tatgttactt ctctagtgag cctcaatact cggttgtttt aaccacaacc
    24901 gagccattta tacctaccga ttgaactttt atctaaagcc tcagtcttaa aatacagttt
    24961 ttttcaatta ttttccctct tttatattta aggattttca ataaaagcat taaaaacatt
    25021 attagcattg ggcgtggcaa tggctgtttc agctacttca tttgcacaaa ccactcaacc
    25081 tactgcggct gaacaaatga acgcagttaa agcctctgtt gccgaaaaag caactgtggc
    25141 taaagaagca acaaaagaga aagcagcgga gttaaaagcc ggttctgcgg aaaaaatggc
    25201 gatagcaaaa gccaaaaccg aatcatttaa agattccgct aaaaacaaaa tggacgcagt
    25261 aaaagccacc acagcagaaa aaacggacgt gattaaagcg aaagccgcat ctgcaagcga
    25321 cacaatgaaa gtgaaagctc aggaagcaaa agccgccacc gcagagaaag ctgcaactgc
    25381 tcatgaaacc ttaaattcag ccaaaaccgc aaccgctgaa aaagcggcct caatgaaagc
    25441 agctgcaaca gaaaaaatgt cagcagtgaa aaaatcacaa aaagtgaata tcaacactgc
    25501 caacgaagag caattacaat cgcttaccgg cattggtgaa gcaaaagcca aagcgattgt
    25561 agaataccgc aaaaaacacg gtaaaatcaa aaatgcagca gaactggcga acattcccgg
    25621 cattggcgat gcaaccattg agaaagttgc tccatattta actttctaat gttctgcaag
    25681 cggttatttt gttgcaaaat tttgcagaaa aataaccgct tgttcatttc acaccttgta
    25741 ttcttaaatt tcacccccat taagtgaatc gtatttcttt caatacagta ttcaataaac
    25801 tcaaaaggaa cagaaaatgg cattagattt caattcagtt ggattagatt cagtagaagc
    25861 aaaaggcggt tacggcatcg gtttacaaat tggtcagcaa cttttaggca gcggaatgga
    25921 cgtaaacgca gaaaccgttg cccgtggtat ttatgatgtg ttaaacaaca atgagccggc
    25981 aatcgacatc aatgaagtga ccgctgcatt acaagaatta ggtcagcgtg cggaagcggc
    26041 tcaagcagaa gcattcaaag cgattgatgc cgaaaacaaa gcgttcttgg aagagaataa
    26101 aaaagcaaac ggtgtaatcg ttaccgacag cggtttacaa tatgaaattt taaccgaagg
    26161 cacaggcgaa aaaccgactg cgacttcaac cgtgcgtgtt cactacaccg gttcattaat
    26221 tgacggcacc gtgtttgaca gctcggtaaa acgcggtcag ccggcagaat tcccggtaaa
    26281 cggagtaatt cgtggttgga cagaagcatt gcaattaatg ccggttggct caaaatggcg
    26341 tttaaccatt ccacaagaat tagcctacgg cgaacgtggt gcagaagcat caattccacc
    26401 atttgcgacc ttagtgtttg aagtggaatt gcttgatatt ctctaatcaa actcaaataa
    26461 aaaacctgtg gaggcatttc acaggttttt gctatttaag ataactatat tatgcagaaa
    26521 aaatcactca tatctcgctt acttaaaaat attttcctct acggcagcct gtttatcatt
    26581 attagcctgg tggttgattg gtatcgctcg ccctccgctc cggcacaatt tgagcaaacc
    26641 gtgcattatg atattcaaca ccagccgaaa gtaatcgccc aattaagcca cgagaagccg
    26701 atgctactct atttttgggg aagttggtgc cactattgcc aagtggtttc gccaaatatc
    26761 caacaattag cgaatgacgg cacggaagtt ttaggtgttg ccctgaaatc cggcgatgaa
    26821 aatgacgtac aagcctatct caatgaaaat ggctacactt tcgctacttt gaatgaccca
    26881 agcggtgatt tctctcgagg ttggaacatt caagccaccc caaccatctt gattatcaaa
    26941 gacggtaaaa tcgcccacca caccaccggc tacactagct atttgagctt aaaattcaga
    27001 ctatggctgg cagacgtgtt gtattaaacg attcctttca tttgcaaaaa aatccactaa
    27061 atccgaccgc ttgtttacac caaaaagaca agcggtacta aattcattgt catcattgtg
    27121 gtgatcgata aaatcagcct cgatgagcca aaaataaagc ctcggttttt gccgttgtcg
    27181 aatttcacca gtaaatttgc cattgctgtg agggcgtaca cttccaccac cgtaaaaatc
    27241 acggtgtaaa tataggcagc ggtcagagtt gcgactttta ccgcaataaa ccaaggggca
    27301 ataatcgcca ccgctaataa cggcgaaatc caaattaatt gctgtttggt gaggttgatt
    27361 ttacgagaaa aagtcgattg caaactgacg gttaaaacac cgttgatgaa taacgcaatc
    27421 ggcgaatgct ccggtgcgtg caggacattg tcgaataaat acggaaaaat cactcagcgg
    27481 cacaaatagc attaagtgag cgaacaaaaa ctctttgttg atgatttcct taatttgtgc
    27541 gaaacgaaat ttcacgccgc cattcacgcc ttccaccgaa taaaacggct tgaccataaa
    27601 cgcaaacaag ccggcttcca aaataaagca gatccaaatc agcgtttcaa tcatctcaaa
    27661 cctaataaac ggaataataa tcagcggggc gagcattgaa gacatactgg tgacttttag
    27721 atatttgcct tgtaaacggg ttttagcagc atattcatcg ccggcaatcg ccagtaaaca
    27781 ggctctggca ttagtgccga ataagcaact ccccaaccca aagcaagtgg ttgccaacaa
    27841 taaaatgagg taattatccg ctgctaaaaa cagcatatag gcaaccacat ctaataaaca
    27901 gcccaataac atcatttttg ccaagccgta gcgatccccc caaatgccgg ctaaaattgc
    27961 caatgcctga ttacaaaaca acagcaatga aaggaaaaaa gcgatttggg cgttatctag
    28021 ccctttctct tttaaataaa agaaaagggg ctgtcctaga taactaacct aaactaccct
    28081 taaccaattg ttttaaaaag gatatttgag attttaagtc actgtgatta aaacgccatt
    28141 cgcattcttt caaatacagc tcaaaatgca ctttgggaat gccgttaaac ttacgcaaat
    28201 ggcgttttgc ttggctccca aaattctcaa ttccattaat gtgattttga ttttcagcga
    28261 agtgtgtgct gtgattaatg cgaaaatggt taaattcact cacatcaagc acatcataac
    28321 ttcgatagaa atcggtgtaa acaatactat cgggtttcac cttttcccga ataatcggca
    28381 aaagtgtggc aaattgcgtg tttggtacta ccacggtata aacctttccg tcccgcttta
    28441 aaagcccgaa cacggcggtt ttgcctgctg caccacgtcc acgtttacct ttacgatgac
    28501 cgccgaaata actctcatca acctcaattt ctccctcaaa catttccaaa tgagggctat
    28561 gttgatagat aagcaaccgc aagcgatgga agtaatacgc cgccgtgttt ttgtttacat
    28621 tgaccaactc agatgctgtt cgggctgtta cccctgcaac aaactgctca atgagtttgt
    28681 tttgtttgta ctgacttaga cgactttttc tcatttgggt atcttaacct aaatggagtt
    28741 tttagtcgtt atctaggaca gccccaaaga aaaacacgct ttgcattgaa aaaaaggcgg
    28801 tggtggaaat aaaacggcgt aggagtaata gtttttctaa agacattcag aatggttgaa
    28861 ttggttaaat aaagagcggt tattctacct tttaagtcaa ccccaatcaa tcggcttttg
    28921 ttttaccttt ggcgtgggtt tcggtatgat aagcgatatg tagtggcggg tcctgtgccc
    28981 gcctaaatac caatgataac ttgcgggcga gcacaggact cgcccctacg ataaacaaga
    29041 aaatgaaaga caccattgtt gctcaagcaa caccgattgg cagaggcggt gtggggattc
    29101 tgcgagtttc cggtccgctt gccgaaaccg ttgctcaagc cgtattaggc aaaacattgc
    29161 caccccgtat cgcaaattat ttgccgttta aagacgaaga tggcactgtg ttggatcaag
    29221 gcatcgcctt attttttaaa gctcccaact cctttaccgg cgaagatgtg cttgaacttc
    29281 aaggacacgg tgggcaggtt attttagatt tactgctcaa acgcatttta caagttaagg
    29341 ggctacgcat tgcccgagca ggtgaatttt ccgaacaggc atttttaaac gataagctcg
    29401 accttgccca agctgaagcg attgcggatt taatcgacgc caccagcgag caagccgctc
    29461 gttcagccct gaaatcacta cagggtgaat tttctaataa aatcaatgcg ttagttgatt
    29521 cagtaattta tttacgcact tatgtggaag cggcgattga cttccccgat gaggaaattg
    29581 actttctggc agacggtaaa atcgaggcaa aattaaatga gattatcgct caattagacg
    29641 gtgttcgccg tgaagcgaaa caaggctcga ttctgcgtga aggaatgaaa gtagtgatcg
    29701 ccggtcgccc gaatgcaggg aaatcaagct tgcttaacgc cttagccgga cgagaggcgg
    29761 cgattgtaac caatattgct ggcacaaccc gtgatgtgtt gcgtgaacat attcacattg
    29821 acggaatgcc gttgcatatt atcgacaccg ccggtctccg tgatgccagt gatgaggtgg
    29881 aacgtatcgg cattcagcgt gcttgggaag aaatcgccca agccgaccac gttttattaa
    29941 tgattgacag caccgagcaa caagccgatc agttccgaca agagtgggcg gaatttctag
    30001 ccaaactgcc ggcaaatatg cctgtgaccg taattcgcaa caaagtcgat ttatccggcg
    30061 agcaggaagg cttaattcag gtggacgatt tcacgatgat tcgcctctct gcccaaacca
    30121 aagtcggcgt tgatttattg cgtgaacact tgaagaaatc aatgggctac caaagttcta
    30181 cggaaggcgg attcttggct cgccgccgtc atctgcaagc attggaaacc gctgccgagc
    30241 atttagagcg agggcatatc cagcttactc aattcttagc cggcgaattg ttagcggaag
    30301 agttgcgtat ggtgcaaaat gccttaagtg aaattaccgg ccagttcacc tctgatgact
    30361 tattaggcaa cattttcagc tcattttgca tcggtaaata aatggcactt tttaaccgct
    30421 ttcaaaccca gtgtgcaacg catttgccga accaaaccgc ctttttaatc ggcttaagcg
    30481 gtggggtgga ttcggtggta ctactccacc tttttgcccg cacctcattt cacgttaggg
    30541 caatttatgt ccaccacggg ctaagcccaa atgcggacga ctgggcagcc ttttgcgaac
    30601 aatattgcaa gcggttaaat attcccttta ttttacaaaa ggtcagagtg gatagctcaa
    30661 acggcgttga aaacggggcg agagaagctc gttaccaagc cattcaacaa cagcttaagc
    30721 ccaatgaagt gttcgccacc gcccaccatt tagatgacca agccgaaact tttttgctcg
    30781 ccctcaagcg aggcagtgga gtcaaaggat tatcggcgat gcaagcggtc acttttttgc
    30841 aaaattttac cgtttttcga ccgctactca cttttactaa gtcagatttg atgggttatg
    30901 ccgttcaaca tcagctaggt tggattgagg acgaaagcaa tgccgataac cgttacgacc
    30961 gtaatttcct tcgcaatagc atcttgccac tactcaatca acgttggcag cacttcagcc
    31021 aaatggttgc ccgttccgct cagcattgtg cagaacagca agccttgata gaagagctat
    31081 taagcaatga actcaagtca agaacagggg aaaaacaaca actcaacatt aacggctttg
    31141 ggcaattttc acttgccaag caacagcaac ttagccgcct ttggctggaa caaaacggag
    31201 tgagaatgcc aagccaagcc cagctacaag cggtcatttc tgaactgatt tttgcaaaag
    31261 ccgataaaaa cccacaggtt aaagtcggcg aaaatattat cagacgctat caacaagcta
    31321 tttacgttac cgaagagatc gccgacattc cgccgtttga ggtagtactt cccacagaaa
    31381 ccaaagtgga attaccctat cagctcggta ctattattcg ccaacaacaa gagatcattt
    31441 gcaaaaaaaa cgggaaaaat caccgcttgc aactgcccca agagctggct caatcaacta
    31501 tttctattaa aatcgggcaa ctaggcaaag taaaatgcta cggcaaggct caacgggaag
    31561 aaatgaaaaa gatttggcaa aatcacggca tacctgtttg ggaacgcaac cgtacaccgc
    31621 tgattttttg gcaagacgaa ttagttggcc tattacctat cctctagggt gaaattgctg
    31681 atgaagcgat ttcaaacggg cttttgccac ttgagtgtaa atctgggtgg tcgataaatc
    31741 actatgcccc agcaacatct gtaccactcg caaatcagcc ccatgattga ccaaatgggt
    31801 cgcaaaggcg tggcgaagca cgtgaggcga gagtttatct gcatcaatac ctgcaagcac
    31861 ggcatagtgc ttaatccgat gccaaaacgt ctgccttgtc atctgctgac ctcgccggct
    31921 cggaaataca acatcagact gctgattatt cagcaaaata gggcgaccgt actggaaaaa
    31981 ctcctgtatc caataattcg cctcctcgcc caatggcact aaccgctctt tatccccttt
    32041 accgataatt ctcaccaccc cttgtttgag gctgatatta tccatcgaaa gcgacaccag
    32101 ctccgttacc cgcagaccgg tggcatacag cagctccagc attgctttat cccgtaactc
    32161 tatcggatct aacgtattcg gggcatctag caaatccatt acctgatctt cacttaagga
    32221 tttgggtaaa gaagcacccc gtttagggga agaaagggtt aaagtcgggt catcttggcg
    32281 ataatgctca aggcataaaa aacggaaaaa tttccttaaa caactcaaca ttctggcaga
    32341 gcttgtcgcc ttatagcctt gctccaaacg ctcgccgaga aaaccttgta agtcgatatg
    32401 atcgagagac agaaatgctt taggttcaga aaaataatcg gaaaaacgct ctaagtcaga
    32461 acgataagag gcaacggtat tttcagacaa accgtgttct tgccatagtg tatcgagaaa
    32521 ttgttcaata atagggtcaa gctcagccat acaagcgggt atattttcag gaaattttgc
    32581 aaattttaac agaattaaat ctataaaaat acaaaagcga tagaaacgaa aaaaccgatt
    32641 agcttatgct aatcggttta
//
LOCUS       DANLUA010000006        31102 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLUA010000006 DANLUA010000000
VERSION     DANLUA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31102
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            203..499
                     /locus_tag="PXB44_000267"
     CDS             203..499
                     /locus_tag="PXB44_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5280790.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            489..905
                     /locus_tag="PXB44_000268"
     CDS             489..905
                     /locus_tag="PXB44_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL5280791.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            905..2563
                     /locus_tag="PXB44_000269"
     CDS             905..2563
                     /locus_tag="PXB44_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL5280792.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2649..3296
                     /locus_tag="PXB44_000270"
     CDS             2649..3296
                     /locus_tag="PXB44_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL5280793.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQYKGSGNCSATFSFDFCRINLLL"
     gene            complement(3280..4218)
                     /gene="ttcA"
                     /locus_tag="PXB44_000271"
     CDS             complement(3280..4218)
                     /gene="ttcA"
                     /locus_tag="PXB44_000271"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL5280794.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4281..4994)
                     /gene="ubiG"
                     /locus_tag="PXB44_000272"
     CDS             complement(4281..4994)
                     /gene="ubiG"
                     /locus_tag="PXB44_000272"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL5280795.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            5059..5439
                     /locus_tag="PXB44_000273"
     CDS             5059..5439
                     /locus_tag="PXB44_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL5280796.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5536..8232
                     /gene="gyrA"
                     /locus_tag="PXB44_000274"
     CDS             5536..8232
                     /gene="gyrA"
                     /locus_tag="PXB44_000274"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL5280797.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDFAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8327..10291
                     /locus_tag="PXB44_000275"
     CDS             8327..10291
                     /locus_tag="PXB44_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5280798.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10373..11131
                     /gene="tcdA"
                     /locus_tag="PXB44_000276"
     CDS             10373..11131
                     /gene="tcdA"
                     /locus_tag="PXB44_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL5280799.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11178..12545
                     /locus_tag="PXB44_000277"
     CDS             11178..12545
                     /locus_tag="PXB44_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL5280800.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12565..14112
                     /locus_tag="PXB44_000278"
     CDS             12565..14112
                     /locus_tag="PXB44_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL5280801.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14335..15369
                     /gene="metN"
                     /locus_tag="PXB44_000279"
     CDS             14335..15369
                     /gene="metN"
                     /locus_tag="PXB44_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL5280802.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15359..16036
                     /locus_tag="PXB44_000280"
     CDS             15359..16036
                     /locus_tag="PXB44_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5280803.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            16059..16892
                     /locus_tag="PXB44_000281"
     CDS             16059..16892
                     /locus_tag="PXB44_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5280804.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            16975..17805
                     /locus_tag="PXB44_000282"
     CDS             16975..17805
                     /locus_tag="PXB44_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5280805.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            17857..18648
                     /gene="metQ"
                     /locus_tag="PXB44_000283"
     CDS             17857..18648
                     /gene="metQ"
                     /locus_tag="PXB44_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL5280806.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(18693..19844)
                     /gene="metK"
                     /locus_tag="PXB44_000284"
     CDS             complement(18693..19844)
                     /gene="metK"
                     /locus_tag="PXB44_000284"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL5280807.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
     gene            complement(20027..20869)
                     /locus_tag="PXB44_000285"
     CDS             complement(20027..20869)
                     /locus_tag="PXB44_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL5280808.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            20929..21246
                     /locus_tag="PXB44_000286"
     CDS             20929..21246
                     /locus_tag="PXB44_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5280809.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            21337..22050
                     /locus_tag="PXB44_000287"
     CDS             21337..22050
                     /locus_tag="PXB44_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL5280810.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            22050..22544
                     /locus_tag="PXB44_000288"
     CDS             22050..22544
                     /locus_tag="PXB44_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL5280811.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            22649..23929
                     /locus_tag="PXB44_000289"
     CDS             22649..23929
                     /locus_tag="PXB44_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5280812.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(24016..25053)
                     /locus_tag="PXB44_000290"
     CDS             complement(24016..25053)
                     /locus_tag="PXB44_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5280813.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            25275..25973
                     /locus_tag="PXB44_000291"
     CDS             25275..25973
                     /locus_tag="PXB44_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL5280814.1"
                     /translation="MENSWKYAFKLITPIFMGYFAAGVAYGMLATNAGMPAWFTIVMS
                     FTVVSGTAQYAAIPFFVSGVGAVSVFLSTLLMSLRFSFYTLNMMEHKPISKAKSLVAI
                     AGLTDEGFAVLSSLPKEQKQALFFKIAVLCVAYWTFSTIVGVLLGDSVANYIPHLDFA
                     LPCLFAILAYEQYKNQKQWKPIVIALIGFLLARQITETSVLLVAILVAIVIVALLPQS
                     SSIKLEKGVENEHK"
     gene            25984..26286
                     /locus_tag="PXB44_000292"
     CDS             25984..26286
                     /locus_tag="PXB44_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL5280815.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(26407..27708)
                     /locus_tag="PXB44_000293"
     CDS             complement(26407..27708)
                     /locus_tag="PXB44_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5280816.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(27841..28815)
                     /gene="secF"
                     /locus_tag="PXB44_000294"
     CDS             complement(27841..28815)
                     /gene="secF"
                     /locus_tag="PXB44_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL5280817.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(28825..30666)
                     /gene="secD"
                     /locus_tag="PXB44_000295"
     CDS             complement(28825..30666)
                     /gene="secD"
                     /locus_tag="PXB44_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL5280818.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(30706..30999)
                     /gene="yajC"
                     /locus_tag="PXB44_000296"
     CDS             complement(30706..30999)
                     /gene="yajC"
                     /locus_tag="PXB44_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5280819.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
BASE COUNT         9184 a         6242 c         6992 g         8684 t
ORIGIN      
        1 ctgaccgcag gcctggagat agaacctaca tataatatgc tctactacat taagccataa
       61 aaaatagaaa atcaagtgtt tgttcaagtt tttatgttat tgaatttaca ataactacaa
      121 aaatttatct taattggctc gattgatttt tacacaaaac atagctaaca tatccaaaat
      181 cactcacatt aggagcttaa aaatgacagc gattactatc cctcaatttg aagaggtgac
      241 ttctaagcaa tttcaatctg agttcggcag aattgttgat atcgtaaaag gcaaaacccc
      301 tgtggcgatt acccaatatg gcagaccgac aatggtgctt tttccttacg atcaagcggt
      361 tgaagctcgg cgattattag caaaacagcg tttgcttgaa atgcttgcag agcgagataa
      421 gaatccgccg gcagctcttg cagaaatcac gcttgaagag ttggataaaa tgattgatga
      481 ggaacgtaat gcactctgat tatttagtga ttgatacgaa tattttgatt agtggtttat
      541 tgttcccaaa atcaaatccc aatttagcag tgaaaaaagc cttattaggc tataccattt
      601 gtgtctcgca agcgactttt gacgaatttt tagaagtctc cgctagaccg aaatttgcta
      661 aatatttcag tattcaggaa agggaagaat ttattgccaa tttgttggat gcagtaaagt
      721 ttgtggaaat taaagaaacg attacagatt gccaagatcc caaagataat aaatttttag
      781 aagtggcgat cagtgcaaaa gcgaagtatt tagtgacggg cgataaaaaa gatctcctca
      841 gtatgaaccc ttatcggaat gttgaaatta tttccgtcag cgaattttta gcggaaactc
      901 gctaatgaaa cggtttctca ctctttttct tctccttttt tcatcttttt cattagccga
      961 aacccaccaa atcggcattc tcgctcagcg tggggaagcc tacacccgca cccattggca
     1021 gccttgggtg gattggttaa attcgcagtt tcccaacgag cggtttgagc tggttccatt
     1081 ggggattaac gaggcgagcc gccgggcgga gttggatttt ctgctgacca atcaagctca
     1141 gtttttctat ttgagtaacc aaaatgtgcg atggcttgcc acgctcaaca gtccgttttc
     1201 ggcaaatggt gagcaagggg cggtgggcag tagcatttgg gtgcgtgccg acagccatta
     1261 tcagcagtta agcgatctga aaaatcagac tatcagtgcg gtggataacg atgctttcgg
     1321 cggttttttg cttgggttgt atcagtttca tcaagcagga atgcggcaaa atcgggattt
     1381 ttcggtgcag tttagcggtt ttccggtgga aaatgcgttg gctttgctgg cggaaaaaca
     1441 ggtggaagcg gcgattgtac cggtctgttt gctggaagag ttggagaaag agggcaagtt
     1501 caagcggtcg gattttcgct taattttgca aaacccacag gcgcagggtt gccttgccag
     1561 cacgccgttg ttgccgaact ggtcgctggc ggcaatggaa aatgtgccaa acgagctggc
     1621 ggtacagctt gccacacgct tgctaaacag ccacaatccc gacctacccc gctggacgtt
     1681 gccgttttct tccgcccaag ccgatcacat tctgcgtgaa ctgtatcgcc acccgcaaca
     1741 aaaaagcctg tgggcaacgg tgctggattg ggttcgtctc aataaattcg gcttgttggc
     1801 ggtggtgttg ttcattttgc tcaaccttgt cgccctgcgt tatcaggtct atcgcaaaag
     1861 caaggcgtta cagcaggcac actgcaaaat gcagcagtat cagcaggaac tgacccgagc
     1921 cgaccgcctt gctttgttgg gcgagatgac aaccggtttt gctcacgaac tgaaacagcc
     1981 gttgtcggcg gtgcgaatgt acgccgaggg gctgaaaagc caaaacagcg atccttatca
     2041 acagcggatt ttggataagc tgattgcgca ggtagatcgg gcagtgaaaa cgatgcagag
     2101 cattcgggac tgggcgcaaa accgcccgag tggtgaaccg caagcggtca ttttgaacca
     2161 attgattact aatgtgatcg agtttgtggc ggtggaaaat cgccagaatg cacaaatctc
     2221 gctgattgcc gaccgcactt ttcggcttaa tttacacgcc acggtactgg aacaagtact
     2281 gacgaactgc ctgctcaacg ccctgcaagc cggtgcaagc gagatatcgg tgcggttaca
     2341 agcggtcgaa aacgggctgg aaattgcaat cgaagacaac ggcggcggtt tttcccctgc
     2401 ccagcttgaa tttccgtttg tgccatttcg caccgacaaa ccacacggct tagggctagg
     2461 cttggtgcta tgccaacggc tgatgcaatc gctcaacgga cggattgtat tggcgaacgg
     2521 ggagaacggg gcgaaggtag tgcttttact ccctcaaata taaatgtagg ggcaggtcct
     2581 gtgcctgccc gtaaatccta tcaaattcag ggcgggcaca ggacccgccc ctacggaaaa
     2641 aagaaaaaat gattattcac attcttgatg acgaagaaag cattttagac gctatgtcgt
     2701 tcctgcttgc cccgcttggg gtggaaatcc aaacgtggca atccagccta gattttttgg
     2761 cacaggcgga tttacaccgg caaggcgtgc tgttactaga tattcgaatg ccgctgccag
     2821 acgggcaaca ggttcatcaa caactgcgag aggcacaatc gaccctggcg gtggtgatta
     2881 tgaccgccca cggtgatgtg ccgatggcag tcgctgaatt gaaaaaaggc gcagtggatt
     2941 ttctgcaaaa gccggcaagt tttgaacagc taaaacaggc gattgcacag gcggaaaaag
     3001 taagtgaaaa agcggtgaaa attcgtgaaa tttcgcaaaa ttacgccaaa ttgacggaaa
     3061 aagagcgtaa ccttgtgcca ctgattatgc aaggtttcac caataaacag atcgcagata
     3121 aattagcgat ttcagtgcgt acggtggaag tacatcgagc caatgtgatg gaaaaaatgc
     3181 aggcggagag tttggcgggg ctggtgcaga tgatcgggga gttacaatat aaaggtagcg
     3241 gaaattgctc tgctaccttt agtttcgact tttgccgtat taatttactt ctttaaactg
     3301 aatcattccc gcttcggcaa aattgttatc gtcatcttcg tataagaagg ttttggcttc
     3361 aattttgaca tcatcaaaag cggtgtcgcc ttctatgcct tcaatggttt gaccgtgttt
     3421 aatcccttta aaatcaaaca atttcggatc gcataagtgg gatggcacga tatcttgcaa
     3481 agcactgaac attgtttcta aacgccccgg gtattggcga tcccaagtat tcagcatttc
     3541 tttcaccact tggcgttgta aattaggctg agaaccacac agattacacg gaataatcgg
     3601 gaactgcttg gcttcggcat atttgataat gtctttttct ttacaatagg cgagtgggcg
     3661 gatcacaatt tgcttgccgt catcagagat cagtttaggt ggcatacttt tcattttgcc
     3721 gccgtagaac atatttaaga ataaagtcgc cagcatatcg tcacggtgat gccctaatgc
     3781 aattttggtt gcacccaatt cggtggcggt gcggtataaa atcccacggc gaagtcgtga
     3841 gcaaagtgaa caggtggttt tgccctccgg aattttctct tttacgatgc cgtaggtgtt
     3901 ttcttccaca attttgtatt ccacgccaat gctttctaaa tagcgaggca atacctcttc
     3961 cggaaatccc ggttgctttt ggtctaaatt gaccgctacc acactaaaat tcaccggtgc
     4021 actttgctgt aaattgagca aaatatctaa cagcgtatag ctgtctttac cgccggaaag
     4081 gcagaccatt acacggtcgc cctcttcaat cataccaaaa tcggcaatgg cattaccgac
     4141 attacggcgt aaacgttttt gtaacttgtt gaaattataa gtaatttttt tatcagattt
     4201 ttgaatatcg tctgtcataa tagttgtact aggcttagaa attagcggtg tatagtaaag
     4261 atttcgcact attttgcaag ttaattattc ggcttcagcg tagcgatata gttacaactc
     4321 acatcattat tgagccaaaa tttttcggtt aatgggttga aatggtagcc tctcatctct
     4381 tggcaacgta aatcagcctg atcgcaccaa gctaacaatt cggcaggctt gatgaatttt
     4441 tcaaattcgt gcgtgccttt tggcaacatt ttcagcacat attccgcccc gataattacc
     4501 aacatatagg ctttaaaagt acggttgatg gtagagaaaa acagcacgcc gtcaggtttg
     4561 agcagggctt tacagctttg aatgatagaa agcggatcgg gaacgtgttc cagcatttcc
     4621 atacaagtaa tcacatcgaa tttttcttga ttacaagcgg tcatttttgc taaaaaattt
     4681 tcaatcgtgg tttgttggta atcaatctct aacccacttt ctttggcgtg ctgctttgcc
     4741 acttcaagcg gttgagaggt catatcgatg ccggtaacaa tcgctccctg ttttgccatt
     4801 gcctcactta aaataccgcc accacagccg acatctaaca cctttttgcc aaataaaccg
     4861 ttcgattttt ctaaaatata ggctaaacgc agtggattaa gcagatgaat cggcttgaaa
     4921 ctgccgtttg gatcccacca ggttttagcc atattttcaa attttgtgat ttcttgctga
     4981 tcgacgtttt gcataaactg ttcctatggt aaatttaggg cattactata acgcatttag
     5041 cctaaaagga aaatcagaat gacccgaatt aaccttgttc cgccaacgga attatgcgat
     5101 caacatctgc ttgccgaaca tcgtgaactt actcggattc cgaatgcggt agcgaaaggc
     5161 aaatttagcc taaaaggaca gccagaagat tacaaactgg gcgagggaca cgttcggttt
     5221 ttcttcaata aactcacgtt tttgaaaaag cgttacgatg cgttacacga ggaatgttta
     5281 gcaagaggtt ttaaggtgca atatttctgg gcggaacatt taccacaaga tccctctctc
     5341 tggctagatt atcagccgac tgaaaccgct ctgactgtaa atcgggagcg aattgcatta
     5401 agaatgccgg caaatccacg ttttagcgaa agaaaatgac cgcttgtacg cctttttgtt
     5461 gctttttatg ctataatctc gccaattttt tcgccttatt taaggctctt ttactaataa
     5521 aattaggaaa tttcaatgag cgaattagcc aaagatatta ttcccgtaag tattgaggac
     5581 gaattaaaaa cctcttacct tgactatgcg atgtcggtga ttgttgggcg tgccttgcct
     5641 gatgtacgag acggtttaaa gccggtacac cgccgagtgc ttttttcaat ggatcaaaac
     5701 agcaacacct ataataagcc gcacgttaaa tctgcccgtg tggtcggtga tgtgatcggt
     5761 aaatatcacc cacacggtga ctttgcggtg tataacacta ttgtgcgtat ggctcaaccg
     5821 ttctcactac gttatatgtt ggtggacggg caaggtaact ttggttctat tgatggcgat
     5881 gcaccggcag caatgcgtta taccgaagtg cgtatgcaga aaattacgca ggaattatta
     5941 accgatctcg ataaagaaac ggtggatttc tcaccgaact atgacggcaa agagatgatt
     6001 cctgatgtat tgccaaccaa aattccggct ctgttagtca atggttcttc agggattgcg
     6061 gtcggtatgg caaccaatat tccacctcat aatttaggcg aagtattaga tggttgcttg
     6121 gcgtatattg aaaatgaaca gattagtgtt gaagagctaa tgcgttatat cccggggccg
     6181 gatttcccga ccggggcgat tatcaacggt cgtaaaggga ttgaagaggc ttaccgcacc
     6241 ggtcgtggca aggtgtatgt gcgcgcgaaa gccagcgtgg aaacaactga taaagggcgt
     6301 gagcaaattg ttgtaaccga actgccatac caagtgaata aggcaaaatt aattgaaaaa
     6361 attgccgagc tggttaaaga gaagaaaatc gaaggcatca gcaaaattga tgactattcc
     6421 gataaaaaag ggatttcgat tgttattgaa gtcaagcgtg atgcagtggg tgaagtggtg
     6481 ttaaaccacc tttattcttt aacccaaatg caggtcacgt ttggtatcaa tatggtggcg
     6541 ttagataatg ggcagccaaa agtgctgaac ttacgccaga tcatcgaagc ctttgttaaa
     6601 caccgtcgtg aagtggtcac acgccgtacg atttttgaat tgcgtaaagc ccgtgaacgt
     6661 gcccatattt tagaaggttt ggcgattgcg ttagcgaata tcgacccggt aattgaatta
     6721 attcgagcct caaaaaccgc agaagaagcc cgtgaaggct tattggctcg cccttgggca
     6781 ttaggcaatg ttgcaccaat gttagaggct gctggtgttg gtgcttcccg accggaagat
     6841 ttacctgaac acttaggagt gcgtgatggc gaatactatt tatctgaagc tcaagccaga
     6901 gcgattttag agctacgctt acaccgctta accggtttag aacacgagaa aatcgttgaa
     6961 gaatacaaag aactcttaac tgaaattggc gaattaatcc acattttaac cagcccagaa
     7021 cgcttaatgg aagtgatccg tgaagagtta gaacgtgtaa aaaccgattt taatgatgaa
     7081 cgccgtactg aaattaccgc agcctctggc gacatcaaca ttgaagactt aatcgcacaa
     7141 gaagatgtgg tggttacgct ttctcacgcc ggctatgtga aatatcagcc gttatccgac
     7201 tacgaagcac aacgccgtgg cggtaaaggt aagtcggcaa ccaagatgaa agaagatgat
     7261 ttcatcgaaa aactcttggt ggcaaacacc cacgacacca ttctttgctt ctcaagccgt
     7321 gggcgtttat atcaattaaa agtgtatcaa ttaccgcaag caagccgtgg ggcaagaggc
     7381 acgccgattg tgaatattct gccgttagtg aaagaagaaa acgaacgtat taccgcgatt
     7441 ctaccgattc caaacggtga atttagtgcc gataagttta tctttatggc aacagctagc
     7501 ggtgtagtga aaaaagtttc ccttgatgcc tttagcaacg tgcgtgcaag cggtctgatt
     7561 gcgttaaaac ttcgtgaagg cgatgagctg atcggggtag atattactga tggtaacagc
     7621 gaaattatgt tgttctcggc acaagggcga gtagttcgct ttgccgaaac agcagttcgt
     7681 gcgatgggac gtaccgccac cggcgtacga ggtattaaac tttctaccac cgaaatttca
     7741 agcgaagaaa ttgaagaagc tgaaattatt gaaactgaca atgaagaggc agaagattcc
     7801 tccagcgtca gccttgataa agaccgtgtg gtttctttag tgattcctcg caccgaaggc
     7861 gatattttaa ccgtcaccca aaacggctac ggtaaacgta cggtgattag cgaatatccg
     7921 gtgaaatccc gagcaaccaa aggggtcgtt tcgattaaag tgaatgaacg aaacggcaaa
     7981 gtggttgcgg cagttcaagt agaggaaacc gaccaaatta tgttgattac cgatgccggc
     8041 acgctggttc gcacccgagt ggcagaagtc agcctcgttg gacgaaacac ccaaggcgta
     8101 cgtattattc gcaccgcaga agacgaacaa gttgttagcc ttgaacgagt ctgcgagcct
     8161 gaagacgatg aaaatgaaag cctagacgca ttagaaaatg ccgaaacagc agaaacatca
     8221 actgaagaat aatcttaact aaacaagcgg tcgaattttc acaatttttt gcaaaaatag
     8281 aaggaaattc gaccgttttt ggatcataca gtagataata aaaactatgg cacttatcaa
     8341 tctttccaac gcctatttag gctttggcga tcatccttta ttggatcata ctgaattaca
     8401 tatcgaaccc ggcgagcggg tttgtttggt ggggcgtaac ggggcgggta aatcgaccct
     8461 gatgaaaatt ctggcgggcg aagttcagct tgatgacggc aagttggtat ttgaaaaaga
     8521 cattgtcgtt acacgtcttg aacaagatcc gccccgccat attgaagaaa cggtgttcga
     8581 ttatgtggcg gaggggattg cccatcttgc cgacttattg aagcaatatc accacatttc
     8641 cctgcaaatg cagaccgatt acagcgatga actgctcgcc aaactttcag cggtgcaatc
     8701 gcaattagaa cacgctaacg gttggcagtt tgaaaaccgc attcaagaca ccttgaaact
     8761 gctggaactc gcccccgaca aacgcctgtg cgaactctca ggcggttggg tacgccgtgc
     8821 ggcattggct cgggcgttgg ttgccgatcc tgatgtattg ctgttggacg aaccgaccaa
     8881 ccacctcgac attgaggcga ttgcgtggct ggaaaatctg ttattggatt tcaaaggcag
     8941 tattattttt atctcgcacg accgtgcctt tatccgcaaa atggcgactc ggattgtgga
     9001 tttagatcga ggcaaattgg tttcctaccc aagcaattac gataaatact tggaagaaaa
     9061 agccgaggat ctgcgagtag aagcgttgca aaatgagctg ttcgacaaaa aactcgccca
     9121 agaagaagtc tggatccgtc aagggattaa ggcaagacgc acccgaaacg aagggcgtgt
     9181 gcgagcgttg aaagcgttgc gtgaagagcg tagaaaccgc cgagaggtac aaggcacggc
     9241 gaaaattcag atcgacaaca ccgcacgcag cggcaaaatt gtgtttgaag tggaaaatgc
     9301 cggctatgaa atcggcggca gaaccctgct gaaaaatttc agcaccacca ttttgcgtgg
     9361 cgataaaatt gccctgattg gagcgaacgg gatcggcaaa accacgctga tcaagctgtt
     9421 acttggcgaa attcagccga catctggttc agtgcgttgt ggcacaaaat tggaagtggc
     9481 gtacttcgac caataccgag tcgatcttga ccctgaaaaa accgtgatgg acaacgtggc
     9541 agacggcaag caagatgtag aagtaaacgg ggtgaaacgc cacgttcttg gctatctgca
     9601 agacttcctg ttcccgccaa aacgggcgat gacaccggtg aaagccctct cgggtggtga
     9661 acgcaactgc ttgttgctgg caaaactgtt actcaaaccg aacaacttgc tgattttgga
     9721 cgaaccgacc aacgatttgg acgtggaaac cctcgaactg ctggaagaga tgctcaccga
     9781 ctatcaaggc accctgatta ttgtgagcca cgaccgtcaa tttgtggata ataccgtaac
     9841 cgaatgctac ttcttcgagg gcgaaggcgt ggtgaataaa tatgtcggcg gctatttcga
     9901 tgccaaacaa cagcaggaaa attacttcgc cgccactgcc gacaagcggt taaattcgag
     9961 taaaaatttg caaaaaatag aggaaaaagc accgcttgcg gagcaagaga aacagaaagc
    10021 cgatcaggaa aaaacgaaag cagagccgac gaagaaaacc aaactttcct acaaagaaca
    10081 gcgtgaactg gacgagttac cggctaaaat ggaacagctt gaagccgaca ttgaagccct
    10141 gcaagccgaa gtaaatagtg ccgaattctt cgccaaagat ccaagctaca ccaacgccca
    10201 gctacaaaaa cttgccgata ccgaagccgc gttagagcaa gcctttgagc ggtgggaagc
    10261 gttggaaaat attaaaaacg ggaatatcta atgtaggggc gaatgattat tcgcccttga
    10321 tggataaaat gattgggcga aaaatttttc gcccctacaa atgtgaagca aaatgacaga
    10381 aagattagat aactacgaac aacgttttgg tggcattggt cgcctttata cgccggaagg
    10441 attggagaaa ctacgccgtt cgcatatttg cgtgattggg attggcgggg ttggctcgtg
    10501 ggcagtggag gcattggctc gctcaggtat cggtaagatc acgatgatcg atatggacga
    10561 tatttgcgtg accaatatca accgccaaat tcacgcaatg agtggcacgg tggctcaact
    10621 gaaaaccgaa gcgatgaaag agcggattga gcggatcaac cctgaatgtg tggtggaaat
    10681 tattgatgat ttcttaacgc aggataatct cgcagactac ctcaatcgag gatatgacta
    10741 tgtgatcgat gcgattgaca gtttgcgggt aaaagcggcg atgattgcgt tttgtaaacg
    10801 caataagatc aaaattatca ccaccggcgg ggcgggcggg cagaccgatc cgaccaaaat
    10861 tcaaattacc gatttaagca aaacggtgca agatccgttg gcggcaaggg ttcgtagcct
    10921 gttgcgtaaa gagtacggtt tcagcaataa aaacaaattc ggcgtggagt gcgtttcttc
    10981 gactcagcaa ctgattttcc cacaaatggg tgaggcctgt gaggtgtcgg caacaatgaa
    11041 ctgtgccaac ggctttggag cggtgacaat ggtgacggca acgttcggct tttttgcggt
    11101 gagtcgggtg attgataaac tattaaaata acaagcggtc atattaaatg aattttttgc
    11161 aaatcaagga gtaaataatg gcaatacatc gagtcaaccc gaaagggagt atggaacagc
    11221 tttcccattt ggaaatggag ttgttggcaa agaatacgca gggtaatttg catcaacttt
    11281 atcgtaattg ctctttggcg gtgctaaatt ccggtgtgca taccgatgac agccgagcgt
    11341 tgttaagcca ataccctgat tttgaaatcc gcttattaac ccgtgaaaaa ggcgtgtcgc
    11401 ttgaattgca taatccaccc gaaactgcgt ttgttgacgg caagatgatt ttgaatattc
    11461 aatatcacct ttttgcggtg ctgcgggata ttgtgtttgt gaatgcgtta aaaaatgcga
    11521 ttagaccgct tgaagagact tctttagaag ccttaaccac gaataccgta ttttcgattt
    11581 tgcggaatgc gaaagcgatt gaaatgaata ccgacccaaa tttggtggtg tgctggggag
    11641 ggcattcgat taatgaaacc gaataccaat attgccgtgc cgtcgggcaa gagtttggct
    11701 tgcgtgagct gaatattgtg accggttgcg gagccggtgt gatggaagcc ccgatgaaag
    11761 gagcggctat cggacacgcc aatcaacgtt ataaaaacgg gcgttttatc ggcattaccg
    11821 agccgtctat tattgcctcc gagccgccaa acccgattgt gaatgaattg gtaattatgc
    11881 cggatattga aaaacggttg gaagcctttg tgcgtttagg gcacgccatt gtggttttcc
    11941 cgggcgggcc gggaacgctg gaagagttgc tgtatattat cggtattaag ttaaacccgg
    12001 ctaaccatgc tcagcaatta ccactgattt taaccgcccc gaaagaatcg gcagactatt
    12061 tctatgccat cgatcaattt attggcgaaa ctttaggcga agaagctcgc agtttgtatg
    12121 aaattattat tgatgactcg gtgttggtgg ctcgtaaagt caagcaagcg atgattgagg
    12181 taaaaagcag ccgttatgaa agtggcgatg cttataattt caactggtcg ttaaaaattg
    12241 aggaagattt tattacgcca tttattccaa cccacgaaaa tatggcaaac ctgaatttaa
    12301 gtttagcaca gccaaaagag cagctggctg cgaatttaag acgggcattt tcagggattg
    12361 ttgcaggtaa tattaagccg gagacctccg atttaattga gaaatatggg gtgtttaaat
    12421 tacacggcga tcgtaaatta atggagcata tagataaact gttggagcgt tttgtggttc
    12481 aacaccgtat gaaaattcca acgggtgagg cgtaccaacc ttgctatgaa attgtaaaag
    12541 ggtaatgcta acaaggggta aggtatgtat ttaaagcata ttgaaatcag tggctttcgt
    12601 ggtattaacc atttatcgct ccagctacga ccaaatatgg tattgattgg cgaaaatgcg
    12661 tgggggaaat ccagtctgat tgatgcatta agcctgattt ttaaccttga gcaggcgttg
    12721 tatcaatttc agttcaaaga tttccatatt caatacaccg atacccccaa ccaagtgcgg
    12781 gacattcaac tggtgtttac cttttgtgaa gagcatcagc acgatttagc ggtgcaattt
    12841 cccgcttatt tggcacaagc cgcgttttat attgataatc accgctgtgt ttcgcttgcc
    12901 gtaaccggaa aattacacgg agagcagatt agcaccgact atcaatttgt ggatcaaagc
    12961 ctaaaaccga tccagataga acaagcggaa aacgccattc ggcagtttat ttaccatcac
    13021 cctgtttatc gcctgcgtga tgcaaggctc aataacaggg tgcataatga attattactt
    13081 aaaggatcgt ctcaacttga agacgatttt caacgtgaat taaacgccct ctcttcgcta
    13141 ctgaaatact attttctaac cagccaaagt cgctatttga tcaaaaccag tatgcaagac
    13201 accgcattgt ggtgggaaaa agtaaaatcc ctctgtttac gcctaaaaca gcagccggaa
    13261 ttggtcgaat ctgctcagca gcatatttct actctatttt tgtttaatcc tagatctaca
    13321 gtaatagaaa agccgattat tttatttgaa gatctgaatg ctcaactaca ccctcgaatg
    13381 attgcgattt tttgggaact gctcggtttt ttacccagcc aacggatcac cactactaat
    13441 tctattgaat tgttatcgca agtgcctctg cgtgaaatct gccgtttggt gcgtcatcac
    13501 gatcataccc aagcctattg gcttgaacgc cacagtttag gcaaggaaga tttgcgaaaa
    13561 ttaacctttc atattcacca taatcgtggc ttggcactgt ttgctaaagc gtggattttg
    13621 gttgaggggg aaacggaggt ttggattttg caagagctgg cgagattatt agatattcat
    13681 ttggaaatgg aagggatcaa aatcgttgaa tttgctcagt gtggcttacg tccgttaatt
    13741 aaatacgcca gagcaatggg gatcgaatgg tatgtgctga ctgacggtga cgattccggc
    13801 aaacgttatg cagatacggt aaaaagtatg gaagaggatt ttgctcaccg gctcacgatg
    13861 ttgccacaaa aagatatcga acattttttc tacaaagcag gcttgcggaa tgtgtttata
    13921 cagcttgcaa attggcgttc acaagaaaaa tatccggtgt cctacatcat taaacgagcg
    13981 attcaacgaa gctcaaagcc tgatttagcc attgcattag ccaatgagat ggggcaacgg
    14041 ggagagcatt caattccaac tttatttaaa caattatttg cagatgtgct ggcattgata
    14101 aacaaagtat agaatacaag cggttaaatt tgacaaaaaa ttgccaattt tgaccgcttg
    14161 tttgcttgtg atgaaaacca atcatacaaa actctaattt atttcaagtg caagctatta
    14221 ttttatgcgt tatgcttatt tggaataaaa aaatcgggtt agttgattga catagcattc
    14281 tagacgtcta aaataaccaa aaataatagt taattgaatg tggataaata gaaaatgatt
    14341 cagctgaaaa atatcagcaa acaatttgag gtaaagggca aaaaaattac cgctctggat
    14401 aatgtaaacc tagaagtacc aagaggcact atttatggtg tcattggggc atcaggggca
    14461 ggaaaaagta ccttaattcg ctgtgtaaat ttattagaac gcccaacaat cgggcaagtg
    14521 attgtcgatg ggcaagattt aacggcaatg tcggataaag agttgattca cgctcgccgt
    14581 aatattgcaa tgattttcca acattttaat ttgctttcat cacaaactgt atttgaaaac
    14641 gtggcgttat cgttgcgttt aagcaatacg ccaaaagagc aagttgagaa gaaagtcaat
    14701 gagctgttgg atttagtggg gttaacagat cgtaaagatg tttaccctgc aaatctttcc
    14761 ggtgggcaga aacagcgtgt agcgattgct cgtgcgttag ccagcgatcc acacgttttg
    14821 ttgtgtgatg aagcgaccag tgcgttagat ccggcgacta cgcaatccat tttgcagcta
    14881 ttaaaagata ttaataaacg tcttggtttg acgattttat taatcactca cgaaatggaa
    14941 gtggtaaaac gcatttgtga ccgagtagca gtgattgata aagggcagtt gattgaatct
    15001 ggctcggtaa gtgaaatttt ctcgaatccg aaaaccgaat tagctcagaa atttattcaa
    15061 tcgactttcc attttgaatt accgcaagaa tataccgagc agctttcaag ttcgccgact
    15121 gaacatagta agccgattat taaatttgag tttaccggac gttcggtgga tgcacctttg
    15181 ctttcaatgg catcgaagaa attcggcatt gattttagta ttttgatgtc gcaaattgat
    15241 tatgcaggcg gggtcaaatt tggatttgta attgcagaag tgagtggtaa tccggattcc
    15301 attgcagagg caaaatttta tttaattgat aacaatgtaa aagtagaggt gttaggctat
    15361 gtgggctgat tttatgcagg agatgacgcc acagatgtgg agcttagtat gggaatctac
    15421 ttttgaaacc gtttatatgg gctttgtggc aacggcattg gcggtagtca tcggtttacc
    15481 gattggtttc ttggcgtttt taaccggtaa aggcgaaatt ttagaaaatc cgagattaca
    15541 ccaagtgtta gatgtcgtta ttaacatcgg gcgttcgatt ccgttcatta ttttattgat
    15601 tattttgttg ccatttactc gcttagttgt gggcacaaca ttaggcacaa cggcagcgat
    15661 tgttccttta agtgtttcgg ccattccgtt ttttgcccgt ttaacttcaa atgcgttgct
    15721 tgaaattccg tccggtttaa cagaagcggc aaaatcaatg ggagcaacaa attggcaggt
    15781 agtgacaaaa ttttatttac cggagtcatt accgattttg attaatggca ttacgctaac
    15841 gctggttgct ttaattggct actcagcaat ggcgggggct gttggtggcg gcggtttagg
    15901 caatttagcg attagctatg gcgaacaccg taatatggtg tatgtgaaat ggattgctac
    15961 cattattatt gttgcgattg tgatgattag ccaaaaattg ggtgatcatt tagcaaaacg
    16021 ttacgatcat cgttaatttt atttaaaaag aggtttctat gagtttcaag aaaattttag
    16081 gcgttgcatt ggtttctgca ttagcattaa ccgcttgtaa agaagagaaa aaggcagaat
    16141 ctaccgctgc tccggcagct caagctccgg ctaaaatcaa agtaggggta atgtcaggcc
    16201 ctgagcatac cgttgcagaa cgtgcagcac agattgcaaa agaaaaatat ggtttagaag
    16261 ttgagttcgt tttatttaac gactacgctt taccgaatac tgcagtaagt aaaggtgatt
    16321 tagatgcaaa cgcattccaa cacaaaccat atttagataa agacagccaa tcaaaaggtt
    16381 taaacaactt agtgattgtg ggtaacacct ttgtttatcc gttagcgggc tactctaaaa
    16441 aagtgaaaaa tgtgtctgag ttagcagaag gtgcggtaat tgcagtacca aatgatcctt
    16501 caaacttagc tcgtgctttg attttattag aaaaacaggg gttaattaaa ttaaaagata
    16561 ataccaattt attctcaact tcagtggata ttatcgaaaa tccgaaaaac ttaaaaatta
    16621 aagaagtgga tacttcaatt gccgcaaaag cgttagatga cgtagattta gcggtggtaa
    16681 ataacactta tgccgggcag gtaggcttaa atacccaaga tcacggtgta tttgttgagt
    16741 ctaaagattc accgtatgtg aatattattg tggctcgcca agacaataaa gatgcagcta
    16801 atgtacaaaa ctttattaaa tcttaccaaa ccgaagaagt gtaccaagaa gcacaaaaac
    16861 actttaaaga tggtgtagta aaaggttggt aatttagctg atttttactt aaatggcgat
    16921 taagcgctag gttaatcgcc attttcatct ttaaaatata ggaaggagca aacaatgaac
    16981 tttaaaaaat tattaggtgt ggcgttagta tctgccttag cacttactgc gtgtaaagat
    17041 gaaaaagcac aagcacctgc tacaacagct aaaactgaaa acaaagcccc attaaaagtg
    17101 ggtgtgatga ccggccctga agcccaaatg accgaagtgg cagtgaaaat tgcaaaagag
    17161 aaatatggct tagatgtaga gttagtgcag tttactgaat acactcaacc aaatgccgca
    17221 cttcattcta aagatttaga tgctaacgcg ttccaaacgg tgccttattt agagcaagaa
    17281 gtgaaagatc gtggttataa attagcgatt atcggcaata cgctagtatg gccaatcgcg
    17341 gcttattcta aaaaaattaa aaacatttcc gagttaaaag acggagcgac tgttgcgatt
    17401 ccaaacaatg caagtaatac tgctcgtgcg ttattattgc ttcaagctca cggtttattg
    17461 aaattaaaag atccgaaaaa tgtgtttgct accgaaaacg atattatcga aaacccgaaa
    17521 aatatcaaaa tcgtacaggc ggatacctca cttttaaccc gtatgttaga tgatgtagaa
    17581 cttgcggtaa tcaacaacac ttacgcaggt caagctgggt taagtccgga taaagacggt
    17641 attattgtgg aatctaaaga ttcaccgtat gtgaatttag tggtaagtcg tgaagataat
    17701 aaagatgacc cacgcttaca aacttttgtg aagtcattcc aaaccgaaga agtattccaa
    17761 gaagcgttaa aattatttaa cggtggtgtg gtgaaaggtt ggtaatcagt tggacggcat
    17821 attctgccgt ccttttctta aaaggagaaa cagataatga aaataatgaa attagccggt
    17881 gcagttgcaa ttttctcgct ttttttaacc gcttgtaatg ataaagccga aaagttgaaa
    17941 gtcggtgtga tttccggccc tgaacataag gtaatggaag tggcggcaaa aattgcaaag
    18001 gaaaaatata accgtgatgt tgaattagtg gtatttaccg attatgccac gcctaatgca
    18061 gctttagata aaggcgatct tgatttgaat gctttccagc ataaacctta tttagataac
    18121 caaattcagg aaaaaggcta taaattagtg ccggtcggca atacttttgt ttatccgatt
    18181 gcggcttatt ccaaaaaaat taaatcgctg gcagagttga aagatggtga tactattgca
    18241 gtgcctaatg atccgactaa tttagcccgt gctttaattt tattggaaaa acaagattta
    18301 attaagctgc gagcagatgc aggcttaaaa gcaaccagtg tggatattat tgaaaaccct
    18361 cgtaaattgg tgatccaaga aattgaagca ccattattgc ctcgaacgtt ggacgatgtt
    18421 gccttttcga ttattaatac tacctacgca gggcaaaacg gtttaacgcc aaccaaagac
    18481 ggaatattcg ttgaagacaa ggattcgcct tatgtgaatt taattgttgc ccgtgaaaat
    18541 aaccaacatt ctgaagccgt aaaagatttg gtgaaagcct accaaacaga agaggtgtat
    18601 aacaaagcga atgaagagtt taaaggggcg atgataaaag gctggtaatt gaagataaat
    18661 aaggcataga ttcctatgcc ttacttttta tgttacagac ctgctccggc tcttaatgct
    18721 tcggctttgt cagttttttc ccaaggaaaa tgtttacgac cgaagtgtcc gtaggcagcg
    18781 gtttcacggt agattggttt gattaaatct aacattttga ttaagccata cgggcgtaag
    18841 tcaaagtttt ggtagattaa tttcacaagt atttcgttgc tcactttacc tgtgccgaag
    18901 gtttcgacca taattgatgt tgggtcagcc accccgatag cataagaaag ttggatctca
    18961 cagcgatcgg ctaagcctgc ggcaacgata ttttttgcga cataacgagc agcataagca
    19021 gcagagcgat ccacttttga cgggtcttta ccagagaatg caccaccacc gtggcgagcc
    19081 gcaccaccgt aggtatctac aatgatttta cgcccggtta aaccacagtc acccattgga
    19141 ccgccaatca caaaacgacc ggttgggttg atgaaatatt tggtgttttg gctgagccat
    19201 tcgcttggta gtaccggttt gatgatctct tccatcaccc cttcaattaa ctctttttgc
    19261 gaaatatgct ctgcgtgttg tgtagaaagc actacggcat caatgccgat aattttattg
    19321 ttctcataag caaatgttaa ttggctttta gcgtcagggc gtaaccaatc gagtttgccc
    19381 gatttacgca cttttgactg ctgttccatt aagcggtgtg cgtaagtaat tggagccggc
    19441 attaacacct cggtttcatt ggtagcgtag ccaaacataa tcccttggtc gcccgcacct
    19501 tgctctaacg gatcggcacg gtcaacgccc tggttgatgt ccggagattg tttaccaatc
    19561 gcatttaata ccgcacaaga gtgagcatca aagcccatat cagagtgagt atagccgatg
    19621 tcgcaaatca cttggcgggt taagttttca atatccaccc acgcagaggt agtgatttct
    19681 ccccctacta atgccatacc ggttttcaca taagtttcac aagcaacgcg tgctttaggg
    19741 tcttgtttta aaatttcatc taataccgcg tctgaaattt ggtctgcgat tttatccgga
    19801 tgcccttctg aaaccgattc agaagtaaat aaattaattg ccatttgtat tcctctctat
    19861 cttaaatatg gattattaat agtagggacg ggtccagtgc ccgcccataa attatataaa
    19921 atgtttggac aggcacagga ccggtcccta taaaatttgt ttggaagtgt ttccgtctag
    19981 acgtctataa tatatttttt gaacgtttta ggcaaataga atttgtttat ttggctaaat
    20041 atgcccttaa accagccaac gcatttttgg catcttctac ccctaaatcg tacattgctt
    20101 gtattttatt tacatccgtt tcaaggcgac tgattttgac tgttctgctt ggacgaatca
    20161 caaaaatctg ttgtttttca ttgagttgaa taatctgctc aacggcttga ttatattccg
    20221 catagcggtt ttcccagcgt tgcactaatt tcggatattt tttataaaaa cgtttaaaca
    20281 gccacatcga gcttggtttc ttacggtatt caagtgggcg agtaagcacc acgataattt
    20341 tgtcatatcc catttcaata aatttctgca ccggaatact gtctgacaca ccaccgtcta
    20401 aatatttttt accctcaatt tcgactattt tggatacaat cggcattgag gaagtagctc
    20461 gaaaggcttc catttgctcg aacacattgt caattttgat gtattcgggc tcaccggttt
    20521 cgacattggt tacagtaacc caaaagtcca taccggaatc ctcaaaggct tgttggtcaa
    20581 acggatccag tttggaaggg agttcatagt aagcaaaatc ccgattaacg atattgccgg
    20641 tggtaagcaa gctgtgtaac cccatatacc gcttatcttt agcgtatttt ttgttgtatc
    20701 gcaacgccct acctttttgt tttgaaggca aattcacgcc aaataacgca ccggctgaaa
    20761 caccgattgc tccatctata tgaatatgtt catctaaaaa tacatctaac acgccggcag
    20821 taaaaatacc tcgcaaacca ccgccttcca gcactaatcc aactttcata tcattctccc
    20881 tgtaatttgt gtattataac ccgaattatc ttgctaggag aaatcacaat ggctgatgca
    20941 gaatttatgg atttcctgtt agatcaacta agcccgattt ccggtttgcg ttcgaaaaaa
    21001 atgtttggtg aatattgctt gttttttgat aataaaattg tcgctattat cagcaaagat
    21061 tatcgcattt ttgtcaaagc taatccgcaa accttgccgt tattcttagc tgaaaatgcc
    21121 gaacaatttc actattttgc taaaggaaaa gtgcaaaaaa tgcactactg gagcattccg
    21181 gaattagcga ttgaagaagc cgatgaactg gtcaaatgga ttcggctagg cttacaagcg
    21241 gtgtaattta gcgaaaattt tgtaaaaaaa cgctaaaata tgcccgcttg ttaggggggg
    21301 ggacctaata tttctatact ttaagatttt atatttatga accaaaaaaa attagtttct
    21361 ttgattgtcg ttattgtgtt ggcaatcatt tctcaaattt ttagcttttt tgataaaaaa
    21421 gagccttcaa aagcacaaga aaaatcgacc cagagtagtc agtctcatca agtttcaact
    21481 tcatcactag ggaactatga tgtgcagatg aaaaacgacc gtttgaaaca gcatattgta
    21541 gataccgatt attacacctt agcgttgtcg tggtcgccgg cgttttgcca aaaacaaaag
    21601 cgaaataatc acggtaaaat tccgtctcat ttggaatatc aatgtggtgg ctcagcggat
    21661 tttggttggg ttattcacgg cttatggccg caaagtggca aggcaagaag tgttgatgaa
    21721 caccctcgtt attgccaagg cgatttgccg atggtggcag agcagacgat taagcaatat
    21781 ttacctgaat cgccgggggc aactttgttg caaggcgaat gggaaaaaca cggtgcttgt
    21841 gcctttaacc aagcggatga ttacttcgcc aaacaaaaag agctatttaa tcagctcaaa
    21901 ttaccggata ccgaaatgtc taaattagca ctgtttaact ggatgcgagc caataaccct
    21961 catttgcaaa atgtctattt agggggtagc aaagatgagc tgtatatttg ctatgataaa
    22021 gcatttcaac caatggattg cccacgctaa tggcagatct tcgccaactg aaaatgcagg
    22081 ttcagcgcca attaaagcgt gatcttgaac gtgcaaatca ctattttaat accactttta
    22141 cgccaccaag tattagctat gcggtgcgtg gcgtaaaagc cggtgtggct tatcttgaac
    22201 gcaacgaagt acggtttaat cctgtgttat tgcaagaaaa tgaacaggca tttatccgcc
    22261 aagttgtgcc gcacgaactg gctcatatct tggtttatca acattttggg cgggtacaat
    22321 ctcatggtaa agagtggaaa atgatgatgg aaaccgtact gggtgtgccg gcggaaattt
    22381 accattgttt tgatacccaa agtgtgcaac agcaatttag ttaccaatgt gcctgccaga
    22441 ctcaccagct ttcgattcgc cgacacaatg ccgtgatgcg agataaaaaa agctatattt
    22501 gccgtttatg taaaactcaa ctccattttg tagaaaaaag ttaatgtgga gaaaagttaa
    22561 gagaatgtta cgatttagct agacattcct gtttaaacgc ctacaatttg ccgtagtttt
    22621 tatttttaat tttatttccg gagtatttat ggaaacaatt gtaattgtag gtggcggtgc
    22681 aggcggtttg gagcttgcaa cctatctagg caacaaatta gggcgtaaac aaaaggcaaa
    22741 agtggtgctg attgaccgca atgcaacgca tttatggaaa ccactgttgc acgaggtggc
    22801 aaccggtact attgatgagg gaactgatgc gttaagctat cgggctcacg ctaaaaatca
    22861 tagttttgaa tttcagcaag gtacgctggt ggcggtagat agagagaaga aaacggtttc
    22921 actcgccccg atttataatg atcaaaacca acttctcgtt aaagaacgcc atatcagtta
    22981 tgacaaatta gtgatcgcga tcggcagtaa atccaatgat tttggcacca aaggggttgc
    23041 agaaaattgc attttcttag atggctcaga gcaagcaaaa gatttccaaa aacgaatgat
    23101 ggagctgttt ttacgttttt cacatagtga aaataaagat gtaaaaattg cgattgtggg
    23161 ggggggagca acaggtattg agctttcagc ggagctttac cacgtggtga aaaatttaaa
    23221 ttcttacggc tttggtaaat taaaccgtgc cagcttgaaa gtcactttaa ttgaggcagg
    23281 ccctcgtttg attccggcat taccggagaa agtgtcggtt tctgctttcc acgaattgaa
    23341 aaaagcgggt gtttcggtgc gtttaggtac gatgattacc gaagctcgtc cagatgggct
    23401 aatgacccgt ttaggtgaga aaattgaggc ggatattatg gtttgggcgg ctggcgtgaa
    23461 agcccctgaa attacccgtg aatttggttt tgaaactaac cgcttgaacc aaattgagat
    23521 taaagacacg ctacaaacca ccgttgatga gtcagtgttt gtcattggcg attgtgcatc
    23581 attaattcaa gatggcaagc caattccacc cagagcacaa gcagcccacc aaatggcaac
    23641 ccgatgtggt aaaaatattg tggcattgat agaaggaaaa gagatgaagc cgtttcgatt
    23701 taacgataaa ggctcactgc tttccttctc aaaattcggc acggtgggta atttaatggg
    23761 gaatttagtg agcggtgata tgtttattga aggtaaaatt gcccgcttgg cttacctctc
    23821 gctttatcga atgcaccaag tggcattaca cggctgtgtc aaaaccggct tgattgtgtt
    23881 ggtagggcaa attaaccgtt tcttaagacc aacaatgaaa ctacattaaa caaaaatccg
    23941 catcaaatga tgcggatttt ttatggcaaa ataacaagcg gttattttct tgcctatttt
    24001 tgcaaatact cacgcctagt ttgctaattt aatttccgcc aaccatttat caattaagcg
    24061 tttgccctct tcgctggaac ctgctttttg gtaatcaatt tccacaaagt ctaacgcttc
    24121 ggctttggtt gattgtggag caacttcggc gttgatgttt gttggggttt ggtaaacacc
    24181 gtgttttttc caaggaattt cctgtgcttc ttttgaaagc acccaatcca taaagagttt
    24241 ggcgttatct aaattacggg catttttgat aatgctgaca ccgcctaacg catagcccac
    24301 tttaccttcc ggaataatgg cttcaatagg cgcacctttt tctttttcag tggcataact
    24361 atgtacaaag cctaccgtaa ccgcactttc accacgagca agattgctgg tgactagggt
    24421 gcttttcaca tattgcgaaa cgttattgtt gagttttttc agataatcaa aggttttctc
    24481 ttcgccccat aaggttgaaa gagtggtcat aaacgtataa gtcgtgccgg agctacgagg
    24541 atcaggcagt tgtacttcac cttttaagcg aggatctgat aaatcttccc attttttcgg
    24601 tgcctcaatg cctagtttgg cgagtttttc ggtattcacg cctaacccta atacaagcat
    24661 ataaacgata gaggtaaatt taccttgttc actttcaact aaggatttaa attgtggcag
    24721 aatttcagct tgttttggtg aacggtaggc ttccagtaag cctaattcgc ctgcttgaaa
    24781 gtgaggttca atcgtgccgc cataccaaat atctgcctga gggttcgatg cttccgcttt
    24841 tacttttccg aaaattgtac cggttccgcc gtgaatgaat ttagtttcga cattatattt
    24901 gtgtgaaaat tgcttggtca tttcttcaca aacattattt tgtaccgagc aataaacagt
    24961 taaacgacct tctgcttgtg ctgcttgagc tgataagaaa atgccagagg cgattaagat
    25021 cgattttaat gagattaatt tgagtacgtt catcactttc ctgaaaacat aaaataaaag
    25081 gtagtgaagt ctagcatttc aaagcgctgg ttgaaaatag ttaattgcaa tgaagtaggg
    25141 caattgtgcc tattacttta atatttacca actatttgca agttttagca aaaaataacc
    25201 gcttgtagta gctcttgcta ttttgtcaaa attcagaata ataaatgcct ttagtctaat
    25261 gttaaatttt agttatggaa aatagttgga aatatgcttt taaattaatc acgccgattt
    25321 ttatgggcta ttttgcggca ggggttgctt acggaatgct tgccaccaat gccggaatgc
    25381 ctgcgtggtt tacgattgta atgagtttta ccgtggtatc ggggacggca caatatgcag
    25441 cgattccatt ttttgtctcc ggtgttgggg cggtgtcggt ctttctcagt acgcttttaa
    25501 tgagtctacg ttttagtttt tacaccttaa atatgatgga gcataagccg atatccaaag
    25561 cgaaaagtct ggtcgctatt gcaggtttaa ctgatgaagg ctttgcggtg ttatcttcat
    25621 taccgaaaga gcaaaagcaa gcattattct ttaaaatcgc tgtgctatgt gtggcttatt
    25681 ggacattttc cacgattgtc ggtgtgttgt taggcgattc agtggcgaac tatattccgc
    25741 atttggattt tgctctgcct tgtttgtttg caattttagc ctatgagcaa tacaaaaacc
    25801 aaaaacaatg gaaaccgatt gtgattgcgt taatcggatt tttacttgct cgtcagatta
    25861 ccgaaaccag cgtgttattg gtggcaattt tagtagcgat tgtcattgtg gctttattac
    25921 ctcaatcttc ttcaatcaaa ctagagaaag gagtagaaaa tgagcacaaa tgatactttg
    25981 ataatgtttg cagcattagt gctcggctcg caaatttgtc gttttttgcc ggaattttta
    26041 ccgaaaaagg tgttatcttc gcccattttg caaaaattaa acaaaatgtt accgcttgtg
    26101 attatgcttt tattgctttt aacgagccta aactttccta aaaacgggga aggctttaac
    26161 ttaatattgg cacaaattct ggctttaata ggcgtattag gtagctatgt ttggcttaaa
    26221 aatacctttt taagcgtagc gttggggatt ttcggtgtga atgtattttt atccctgtta
    26281 ggataaatac caaaggtcat tttataggag cgaaacacct tcgctcaatc attaacagat
    26341 gtggggcgaa aaatttttcg ccccttattt atcacaagcg gtctttttct ttcaattttt
    26401 tgcaaattaa atcgctaaat caaacgcata gaaggcaact aaaataatcg caataattac
    26461 tgtacctagg tttaatttat tggcttcgcc tgaaattaag cgaccaatca caagggtggc
    26521 gaaacctagc ataatgcctg tcacaatgtt ggcggttaat acgatgaaca ccgcacagac
    26581 taaacctgac attgcaccta caaaatcgct gaaatctaat ttagagacat tacttagcat
    26641 taataagccc acatacatta atgccggtgc ggtggcatag cttggtacta agaatgcaag
    26701 aggttggaag aacagcataa ataagaaacc gataccaacc actacagcgg ttaaacctgt
    26761 tttaccgcca acggctgtac ctgctgccga ttcaatatac actgccgctg gtgctgtacc
    26821 aaataagccc gataaaattg atccgaatga gtcagaagtt agggctttat cgccattgat
    26881 aatttgtccg tctttatcta ataaaccggc ttgccctgca accgcacgga tagtacctgt
    26941 tgcatcaaaa atagcggtca ttactaacgc aaacactacc ggtaaaatgg cggtatttaa
    27001 tgctcccata atatcaagat ttaggaattg cgaattttca ccaaaactcg gcattttgaa
    27061 aaagcctgtg tattttacgt tagggtcaaa aattaaacct acgatggtaa tagcaataat
    27121 gacccataaa atactgcctt ttactttcag cttttctaac ccgataatgg cggctagacc
    27181 gattaatgac actaaaaccg gaaatgaagt gaagtcccct aatttaaccg gaatatccat
    27241 accgctattg accactaatt taacgttagt ggcggcaata atcagtaaga ataaaccaat
    27301 accaattcca gcaccgtggg caatgctgct tggcaaatta cgtaaaatcc aagtacgaat
    27361 accggtagca gaaatcaagg taaatactac gcccattaag aaaatcgccc cgagtgcaac
    27421 aggaatagaa acttgttgcc ctaatactaa gctaaaggca gtaaaagcag taagtgaaat
    27481 cgcacaaccg attgccattg gggcatttgc ccataaaccg attaagatag agccgagacc
    27541 tgaaactaaa caggtggcaa taaataccga ttcggcagga aagcctgctt ggcttagcat
    27601 tcccggcaca acgatgacgg aataaaccat ggctaaaaag gtagttaaac cggcgatgat
    27661 ttcttgacga gcgtttgaac cacgctcctc aaatttaaat aacgacataa gaacctcaaa
    27721 agaagaaaat gaaaggggaa attttaggat gcggaatttt acagaaattt aaaataatta
    27781 gcaaacgttt gcgtaaaaag tgaaagaaaa gcaggaataa atcctgcttt tgtttgatgg
    27841 ttagtaatcg atgaaagcgt cttgatctgc cccttccttt tcaactttag gctgaaccat
    27901 atgttcacgt tttagcccaa gttgtagtgc cacaccaatt gcaatgtaga tggatgaata
    27961 agtaccgaaa ccgataccga tcaataatgc aagtgagaaa ctatgaagcg ttggaccacc
    28021 tagatagaat aaggctagta ccacaaagag ggtggttact gatgtcatta aagtacgcga
    28081 aagtgtttgg ctcaaggaaa tatcgataat ttcaattgag ctggagcgac gaactttaga
    28141 aaagttttca cgtacacggt caaacaccac aattgagtca tttaacgagt aaccgacaac
    28201 agataaaatc gctgctacaa aggtcaagtc aatttcaatc tgtaagaaag agaagattcc
    28261 gatcgtcact aatacatcgt gaaataaagc cacaataccg cctaccgcta aacgccactc
    28321 aaaacgtgtt cccacgtaaa taagtagcat agcaagtgtt gccaaagtgg cataaatagc
    28381 cccttgggtt agctcttccc ccacgtttgg gccaacgaat tcaatgctct tgatagtcgc
    28441 attcggatct aatttttgat ggatcatctc catcacttta ttcccaacag acatatcacc
    28501 ggcactggcg gcaagtcgga tcattaaatc tttttgactg cctgtggttt gcactaaggc
    28561 attgccgtag ccgctatttt caagctccgt acgcagtttt gctaaatcag caggttgtga
    28621 aaattgagtt tcgattactg tcccgccggt aaaatctaac ccccagttaa agccttttgt
    28681 cgcaatagta aaaatgcata aagcagttac aatcagcgaa aaaatgaatc cgacatagcg
    28741 gtatttcata aaggggatta atttataagg cagtttaatt tctgctacat cttgttgtac
    28801 attttcaata gccatttttc caccttaaat ccaaagtttc ttcacgcgtt tgccaccgta
    28861 aacccagttt acaagcattc gagtacctgt aattgcggta aacatagaaa taattacccc
    28921 taatgcaagc gttaccgcaa agccttttac cggacctgta cctacaccat aaagcacaag
    28981 agaagtcaaa atagtggtga ggttggagtc aaaaatactt gagaatgcgc cattataacc
    29041 ttcgtggatt gcttgttgaa ttgggcgacc gttacgaatc tcttctttga tccgctcgta
    29101 gattagtacg ttggcatcaa ctgacatacc cacagcaagt acgataccgg caattcccgg
    29161 catagtaagg gttgcaccga ttaatgacat taaaccaatc gttaagatca tattcacaat
    29221 caaggcaaga gaggcaaata taccaaatac tttataaaag gctagacaga acaggatcgt
    29281 taagcctaag cctaaaatac tagcttccat accttgttta acattatctg ccccaagaga
    29341 agcaccgata gtacgctctt ccacaatcac gattggagca atcaacgcac ctgaacgtaa
    29401 taataccgca aggttttgag cttccgctgg tgaattaatc cctgtaatct ggaattgatt
    29461 acctaaacgg gtttgaatgg ttgcaacgtt aattacctct tggtgttttt ctaaaatgac
    29521 tttaccgtta gcatctttac gacctgaatc tttaaattcg gtataaagtg ttgccatcgg
    29581 tttttggatt gccattttag taatttcacc catcatctcg ccgccttcgg tatcgagtcg
    29641 gatactcact tgcggcaagc cacgctcatc agtgcctgag gttgcatcaa taatatgttc
    29701 accccctaac gaaggtttac ggcgtaatac aactggctcg ccatcacggg tgttttgcag
    29761 tcctgaatca gccggcacaa tgcctttcgc cgcagaggcc acattggctt cggtattgac
    29821 taaccggaac tcaagggttg cagttgcccc taaaagctct ttggcacgag cggtatcttg
    29881 cacacccggt aattcaacta cgatacgatc tgcaccttga cgctgaatgg tcggctcaga
    29941 aacgcccaat tcctcaacac gtttacgcaa aatagaaagg ttttgttcga ttgccatcgt
    30001 gcgagattca gaaagagctt gtgctgatgg cgttaaggtg actttattat catttaccaa
    30061 aatatcaagc gttgggtgag agagacgcac taaacgcact gctttttcgg cggtatcgtt
    30121 atcggcaaat tcaacctcag ttgcaaaatt ttcgcctttt ttgaccgctt gatattgcaa
    30181 tttttcttta cggaaatcac tacgcaggct gtcttgcaaa gtgtcttgtt gttttgcaag
    30241 cgccgtgttc atatccactt ccattaagaa acgaacaccg ccaagcaagt ccaagcctcg
    30301 cttcatcggg ctaccgccaa tgtcagacaa ccactgtggc gttgctggtg caaggtttaa
    30361 ggcaacagag tagttattcc ccaatgcttc tgagattttt tctttggcag gcaattgctg
    30421 ttcgctgctt tcgagacgaa ccaaaatcga gccattttct aacacggcag atttcggctg
    30481 aatattcatt gctgaaagtg tggattgcac ttgagacagt gtctcaggtg tggcttgttg
    30541 cccacgagta ccggaaattt gtaccgaagg atcatcgcca tataaatttg gaagggcata
    30601 taaagcacca atggcgatca cgaagatcac cattaaattc ttccatagag ggaaacggtt
    30661 taacacaata actccccgaa tgttaaagtt ggataagagg gttgattaaa gtgattttaa
    30721 tgaacctttt ggtaacaccg caacaacgaa atcacgtttg atggtcactt cagttgtgtc
    30781 atttaatgca atcacgatgt tatcgttatc agcggtcact ttggtaattt taccgattaa
    30841 accaccgctg gttaatactt catcgccttt tgtcaagctt gccagtaaat cacgttgttg
    30901 tttttggcgt tttgcctgag gacgataaat cataaaatag aaaattgcac cgaaaataat
    30961 caggataaag atcatttcca ttccgccacc ttgttgcata aaaactcctt aaatgtttga
    31021 gtggtataaa aataagtgtt ataaaaaacg cctgttaaaa taccataaat tcaatgaatt
    31081 aacgagtaga aaaattcatc ga
//
LOCUS       DANLUA010000007        28612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLUA010000007 DANLUA010000000
VERSION     DANLUA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            25..999
                     /gene="znuA"
                     /locus_tag="PXB44_000297"
     CDS             25..999
                     /gene="znuA"
                     /locus_tag="PXB44_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL5280820.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(1051..1371)
                     /locus_tag="PXB44_000298"
     CDS             complement(1051..1371)
                     /locus_tag="PXB44_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL5280821.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            1512..3875
                     /gene="rnr"
                     /locus_tag="PXB44_000299"
     CDS             1512..3875
                     /gene="rnr"
                     /locus_tag="PXB44_000299"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL5280822.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            3879..4145
                     /locus_tag="PXB44_000300"
     CDS             3879..4145
                     /locus_tag="PXB44_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280823.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            4368..4826
                     /gene="mraZ"
                     /locus_tag="PXB44_000301"
     CDS             4368..4826
                     /gene="mraZ"
                     /locus_tag="PXB44_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL5280824.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            4842..5780
                     /gene="rsmH"
                     /locus_tag="PXB44_000302"
     CDS             4842..5780
                     /gene="rsmH"
                     /locus_tag="PXB44_000302"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL5280825.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            5799..6110
                     /gene="ftsL"
                     /locus_tag="PXB44_000303"
     CDS             5799..6110
                     /gene="ftsL"
                     /locus_tag="PXB44_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL5280826.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            6114..8174
                     /locus_tag="PXB44_000304"
     CDS             6114..8174
                     /locus_tag="PXB44_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL5280827.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTIHKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            8183..9673
                     /gene="murE"
                     /locus_tag="PXB44_000305"
     CDS             8183..9673
                     /gene="murE"
                     /locus_tag="PXB44_000305"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL5280828.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            9761..11155
                     /gene="murF"
                     /locus_tag="PXB44_000306"
     CDS             9761..11155
                     /gene="murF"
                     /locus_tag="PXB44_000306"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL5280829.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            11176..12258
                     /gene="mraY"
                     /locus_tag="PXB44_000307"
     CDS             11176..12258
                     /gene="mraY"
                     /locus_tag="PXB44_000307"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL5280830.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            12322..13623
                     /gene="murD"
                     /locus_tag="PXB44_000308"
     CDS             12322..13623
                     /gene="murD"
                     /locus_tag="PXB44_000308"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL5280831.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            13623..14801
                     /gene="ftsW"
                     /locus_tag="PXB44_000309"
     CDS             13623..14801
                     /gene="ftsW"
                     /locus_tag="PXB44_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL5280832.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            14920..15975
                     /gene="murG"
                     /locus_tag="PXB44_000310"
     CDS             14920..15975
                     /gene="murG"
                     /locus_tag="PXB44_000310"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL5280833.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            16051..17478
                     /gene="murC"
                     /locus_tag="PXB44_000311"
     CDS             16051..17478
                     /gene="murC"
                     /locus_tag="PXB44_000311"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL5280834.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            17568..18479
                     /locus_tag="PXB44_000312"
     CDS             17568..18479
                     /locus_tag="PXB44_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL5280835.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            18479..19270
                     /locus_tag="PXB44_000313"
     CDS             18479..19270
                     /locus_tag="PXB44_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL5280836.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVIIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            19293..20564
                     /gene="ftsA"
                     /locus_tag="PXB44_000314"
     CDS             19293..20564
                     /gene="ftsA"
                     /locus_tag="PXB44_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL5280837.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            20597..21844
                     /gene="ftsZ"
                     /locus_tag="PXB44_000315"
     CDS             20597..21844
                     /gene="ftsZ"
                     /locus_tag="PXB44_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL5280838.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            21876..22796
                     /gene="lpxC"
                     /locus_tag="PXB44_000316"
     CDS             21876..22796
                     /gene="lpxC"
                     /locus_tag="PXB44_000316"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL5280839.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            22969..24651
                     /gene="recN"
                     /locus_tag="PXB44_000317"
     CDS             22969..24651
                     /gene="recN"
                     /locus_tag="PXB44_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL5280840.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(24698..24773)
                     /locus_tag="PXB44_000318"
     tRNA            complement(24698..24773)
                     /locus_tag="PXB44_000318"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24738..24740),aa:Ala,seq:ggc)
     gene            complement(24853..26292)
                     /gene="gltX"
                     /locus_tag="PXB44_000319"
     CDS             complement(24853..26292)
                     /gene="gltX"
                     /locus_tag="PXB44_000319"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL5280841.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            26467..26542
                     /locus_tag="PXB44_000320"
     tRNA            26467..26542
                     /locus_tag="PXB44_000320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26500..26502,aa:Val,seq:tac)
     gene            26591..26666
                     /locus_tag="PXB44_000321"
     tRNA            26591..26666
                     /locus_tag="PXB44_000321"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26624..26626,aa:Val,seq:tac)
     gene            26719..26794
                     /locus_tag="PXB44_000322"
     tRNA            26719..26794
                     /locus_tag="PXB44_000322"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26752..26754,aa:Val,seq:tac)
     gene            26843..26918
                     /locus_tag="PXB44_000323"
     tRNA            26843..26918
                     /locus_tag="PXB44_000323"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26876..26878,aa:Val,seq:tac)
     gene            complement(27072..28565)
                     /gene="mltF"
                     /locus_tag="PXB44_000324"
     CDS             complement(27072..28565)
                     /gene="mltF"
                     /locus_tag="PXB44_000324"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL5280842.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT         8677 a         5506 c         6386 g         8043 t
ORIGIN      
        1 attaaatgcc ataaggggaa ttttatgttt aaaagaacat taatcggtct agccgtgtta
       61 ggtgcaagtg ttgctcaagc ggatgtatta accagtatta agcctttagg ctttattgcc
      121 agtgccatta ccgaaggggt aactgaaact aaagtattat taccggtaac tgcctctcca
      181 cacgattaca gcttaaaacc ttcagatgtg actcaattac aatctgctca attagtggtt
      241 tgggtgggtg acggcttgga aggcttttta gaaaaaagta ttgaaaaact gccgagagaa
      301 aaagtattaa cccttgaaga tgttgatgcg attgatgata ttgtcgataa agcaagcaaa
      361 aaagaagata aagacgatga tcacgatcat aaacatgaac ataagcacga acacaagcac
      421 gagcataaac acgaagggca taatcacgaa ggacataacc acgatcatca cggacatagc
      481 cacgatgaag attggcatat ttggctctca agcgaggcaa gtgagctggc agcagagcaa
      541 attgcggcac gtttaagcga acaaatgcct gaacacaaag cgaaaattgc ggaaaatctt
      601 gcaaaattta aagcaaatct aaccgctaaa aaagccgaaa ttgcgaaaca attagagccg
      661 gtaaaagcca aaggttacta cacattccac gatgcttacg gctattttga aagagaatat
      721 ggtttaaatt cattgggttc atttaccatt aacccaactg ttgccccggg tgcaaaaaca
      781 cttacagcaa tcaagaaaaa cattgcgaaa aacaaagcac aatgtttatt tgccgagccg
      841 caatttacgc cgaaagtaat tgaaacctta gcaaaagaaa ctaaagtaaa tgttggccaa
      901 ttagacccac tcggtgcaaa agttcagtta agtgcgactg cttaccctga atttttacaa
      961 gttttggctg acgaatttag ccaatgttta acaaaataag ttaaaaatcc cccatttaaa
     1021 tataaatggg ggattttgcg attacatcac ttaagctttt tttaagaatt ctgatttcaa
     1081 cataatttta ccgcaatcga cattatggtc accttgagct aaacggattc ctttgaattt
     1141 tgtacctttt ttcagtactt cagacgagcc ttttaatttt aaatctttga ttaataatac
     1201 atcgtccccg tcagataata aattgccatt actgtctttt acaattagca catcatcatc
     1261 ttgatccgtt tcttcgcccg tccattcata cgcacaatcc gggcaaacaa attgaattga
     1321 gtcgtggtaa gtattttcgc ttttacattc agggcaggtt gggtaaatca ttttaattcc
     1381 tcaaattaat aaagaaccag attatatacc gaaatccagc aaaacgctaa gcggtcatat
     1441 tttgccgatt ttttaccaat cgagctaaaa gtcagttaga atagccttaa ttacacaaaa
     1501 aggaggatac tatgatagtc gatccgaatt atcagcaaga attagaaaaa tacgaaaatc
     1561 cggtaccaag ccgagagttt attttaaata caattcgtga acataacgcc ccaatgagcc
     1621 gtgatgaatt attgtcggct tttcatattg acgatgaaga acgaatggaa ggcattcgcc
     1681 gccgtctgcg tgcaatggaa aatgacggtg aattggtgtt caccaaaggc aaacgctatg
     1741 cgttgccgga aaaaatggat ttaatcaaag gtacagttat cggccatcgt gacggttacg
     1801 gttttttgca agtggaagga cacgaaaaag gggcgactaa aagcgaagat tggtttattc
     1861 ccaacgccca aatggtaaag gtaatgcacg gtgactttgt gctggcacaa ccaaacggca
     1921 cagaccgcag aggtcggaaa gaagtccgca ttgtgcgagt gttggaggcc cgtaagaaac
     1981 agattgtcgg gcgttttttc cttgaaagcg gtatcggttt tgttgtgcct gatgacagcc
     2041 gcgttaacca agatattctc attccggatg aacaccgtat gggggcgaga atggggcaag
     2101 ttgtggtggt ggaattacag gaaagaaaag cgagttttag ccgcccggtg ggaatcatta
     2161 ctgaaattct aggcgataat ttagcgccgg gaatggaaat tgaaattgcc ctacgcaatc
     2221 acgatattcc gcacatttgg ccggaagggg tagaaaaaca aatccgccaa tttagtagcg
     2281 aagaagtgcc ggaagaagcc aagcaagggc gggtggattt gcgagatctg ccactgatta
     2341 ccattgacgg cgaagatgcc cgagatttcg atgatgctgt atttgccaaa aaagagggca
     2401 acggctggcg gttatgggtg gcgattgccg atgtaagtta ttatgttcgc ccaaaaaccg
     2461 cattagattt ggaagcccta aatcgtggta actcggtcta tttcccgaat cgggttgtgc
     2521 caatgttgcc ggaagtgctt tccaatggct tgtgttcact caatccacag gttgacaggc
     2581 tctgcttggt ggcggaaatg tcggtttcca gcaaagggga attacaagat tatcaattct
     2641 acgaagcggt aatgaactct cacgcccgtt taacttacac taaagtgtgg aaaatgttgg
     2701 aaggtgatga agccttgcgt gaacgctatg ctccattagt gccgcatatt gaagaactct
     2761 atgcaatgtt ccaaacctta atgaaagctc gccataaacg aggggcgatt gaatttgaaa
     2821 ccattgaaaa tcaatttatt ttcaatccgc aagggcgaat tgagcgaatt gagccgttaa
     2881 tccgcaacga tgctcataaa ttgattgaag aatgtatgat tttagccaat attgctgccg
     2941 ctcgttttgt ggaaaaagcg aacgaaccgg cgttatatcg gattcacgat aagccgagtg
     3001 aagaaaaatt gctcagtttt aaatcctttg tgcgtgagtg tggcttaacc tgggacgtag
     3061 gcttagatcc aaccccaaaa gattacggtg tactactgga gcaaattgcc gaacgtgccg
     3121 ataaagagtt gattcaaacg atgttgctcc gctcgctgaa acaagcagtg tatgcggcgg
     3181 ataatatcgg gcatttcggc ttggcattaa ccgaatatgc tcattttacc tcgccgattc
     3241 gccgttaccc ggatttactg ttacaccgtg caattaaata tctgatcgaa aaaggcaaag
     3301 gcaacactcg ctactatacc gatggcggtg gttatcacta caagttagat gatatggacg
     3361 aattcggcga taaatgttct gccaccgaac gccgagctga tgaggcaacc cgagaagtgg
     3421 cagattggct gaaatgcgaa tttatgcaag atcatcttgg cgaagagttt gatggcgtga
     3481 tttcgagtgt taccggcttt ggcttatttg taaaactcaa tgagctttta attgacggat
     3541 tggttcatat ttcaacgctg gataatgatt attaccacta tgatgcggac cgacaacgtt
     3601 taatcggtgg cagtgggatc atctatcgtt taggcgatgc agttagagta aaagtaatca
     3661 atgtgaattt agacgagaaa aaaattgatt ttgagttact caccggtaat aagaaaaccg
     3721 gcaaaaccgc caagaaaaaa gcaaaagcga cagaggttcc tgttttcaaa gagcctaaaa
     3781 cggtaaaaaa agccaaaaaa gtcgctgaga aaaaaccggc aaaatctgca aaaaaacgga
     3841 caaaaacgac cgctagtaaa tcaaggaaaa aatagcaaat ggattacaaa aaacaaattt
     3901 tattcagcct tttatttgct ttagtaattg caataataag ctattttttg tttacctata
     3961 tcccgtttga ttgtagcgat ctgagcaaac tcaacgattc acaacgttta gagtgccgaa
     4021 ctgccgaatt tctgggcgtt tattactggc tgccgcacac taccacgctg tttttcggct
     4081 cggtgttatt atattgggcg gtttatggca tgatttcatt agtgcaagca gtgctcaaac
     4141 gctaacaagc agttttgttt ttgcaaaaag ttgcaaatct catctgaata atccttccaa
     4201 gatcaaaggt ctatcaaaaa agatagacct ttattgtgta aataaactaa aactccttga
     4261 tagatgtatc acatatctat aaaattccag caattaagtg ggaaaaagtg gtaaattgtg
     4321 gaacttttcc ttaaatttaa cctcatttaa aaaggtaaat cagtaaaatg tttcgtggtg
     4381 caagttcaat cagcattgat agcaaagggc ggatcgctat tccgacccgt tatcgtgccg
     4441 agctattgga aaaacatcac gggatcttag tttgcactgt ggatatccgc cagccttgtt
     4501 tgttgcttta cccacttcac gaatgggaaa tggttgaaca gaaattgttg gcactatcta
     4561 attttgaccc tgttcaacgc cgtattcagc gagtaatgca aggctttgct accgaatgtg
     4621 aaatggattc ggcagggcga atactgctta gccctacact tcgccaacac gcacaattag
     4681 agcaacaaat tatgctggtt ggacaattaa acaaatttga aatttggcaa gataaacaat
     4741 ggcaagcaca aattgccgaa gatcttgctt ttggcagttc ggcagaaatt ttagactgcg
     4801 aagcattgaa aaacctgtct ctctagtaat tggaaattga gatgagcgag aacccaaaac
     4861 atattacagt attattgcac gaagcggttg acggcttggc aattaagcct aacggtatct
     4921 atattgacgg tactttcggg cgtggcgggc attctcgctt gattttatcc aaactgggcg
     4981 aaaatgggcg attaatcgca actgatcgtg acccacgagc gatagccgaa gctcaaacga
     5041 ttagtgaccc tcgttttcaa attgaacata cggcattttc tgccattccc gaactgtgtg
     5101 aacgaatggg gttggtgggc aaaattgacg gtattttatt ggatttaggt gtgtcttccc
     5161 cgcagcttga cgaagccgaa cgaggcttta gttttatgcg tgacgggccg cttgatatgc
     5221 gaatggatac caccaaaggc ttatcggcag ccgagtggct tgcccaagtt tcggcagaag
     5281 atttagcttg ggtattgaaa gaatttggcg aagagcgttt tgccaaacgg attgcacaag
     5341 cggtcgtttc ttacaataaa tctgcaaatg aaaaaatcag ccgcactttg cagcttgcgc
     5401 aaattattgc ggatgcggtg ccgtttaaag ataagcacaa acaccctgca acccgttcgt
     5461 tccaagcaat tcgcattttt atcaatggtg aattagacga gctggaaaaa gctttaaatt
     5521 ccgcattaac ggtgttagcc cctgaagggc gtttgtcgat tatcagcttt cattctctag
     5581 aagaccgaat ggtgaaacag tttgtgcgta agcatagtaa aggaatggac gtaccccgtg
     5641 gtttaccgat tttagaagca gagctaacca aaaatattcc gctgaaaacc attggcaaag
     5701 cgattatgcc gagcgaagcc gaaattgaag caaatgcccg ctcacgcagt gcggtattgc
     5761 ggattgccga aaaacgctaa cagtaaatag ggaagaatat gtcgaacgaa cgttatccgt
     5821 tacatcaaat catcattgat gatatcttta gccataacaa agtggctctt ttattgttga
     5881 ttggtgtagt gatttcagca gtggctacga tttggataac ccaccagact cgtttatcgg
     5941 tgagcgaaca agggcaactg attcaagcaa accaaaagtt ggaaagccaa tatactaatc
     6001 tgcaattaga agaaaacagc agaagcagtc gggcaagagt tgatgccgcc gctaaatcgt
     6061 tcggattaca gccgattaaa aaagagcaag aaattatttt agttgaataa aaaatggtta
     6121 aatcagttaa attaaaacgt aagcaagaag cccctgtcgc aaaagcagag cctcaacaag
     6181 cagaaggtaa aaaaacaccg agttttttac ctactcgctt tagagtggtt gtgtttttta
     6241 tgctattaat ggttggtggc ttactagcac aaaccgccta tattcagctt tttgactctg
     6301 aaagattaat taaagaggct aataaccgct cacttcgtac taaagaactc caatttaccc
     6361 gtggacgtat tttagatcgt aacgggcgtt ttctctctat tagtgtgcca atgtactcat
     6421 taacacttga cccgagagaa tactttgata ctaagctgcg tcgttctaac gaaagctggc
     6481 gtgccttagc gattgaaatg gaaacatcaa aaggtaaaat tgaggcaaac gttaataaat
     6541 ttattgaaaa gaaaaatttt tcaaaagaaa aagtagattt tgatccacgt tctattttaa
     6601 atactaaaag tgaaggttat tggactcagc ttgcccaagt tacaggtttg gattacaaca
     6661 gtttaataga aaaagtacgg aataacccta gctcggtttt tttacgttta gataacgaaa
     6721 atttagtaag agaacgccag aaattccaag cgttaagcaa agaaatcggc cgttcctata
     6781 atgacataat ggacgagctt tatagcaaaa accgccagcg attcctttac ttatcccgtc
     6841 atcaatctga agcgatcagc gaatatgcaa aagaactaga tattgatgca ttagtgatta
     6901 aagtcgaatc acgccgtttt tacccgctgg cagaagaatc ttcacagttg atcggtttta
     6961 ctgataaaga tgatatgcac ggttcggaag ggctggagcg tagttttgat tccttactta
     7021 tcggcaaaaa cggtaaacaa attatccgaa aagatgccaa aggcaatatt gtggaaaata
     7081 tccgttcgga gaaacagtac gatccgcaag atgttatgtt aagcattgat gaagaattgc
     7141 agtctatggt gtacggtgaa atcaaaaaag cggtacagga aaataatgcg gaatcaggca
     7201 ctgcggtttt ggttgatgtg caaaccggcg aaattttggc aatggcaaat gccccttcat
     7261 tcaacccaaa caaacgggat agttttaaac ctgaattaat gcgtaaccgt gctattaccg
     7321 acacttttga accgggttca acggtgaaac cttttaccgt attaaccgca ttacaaaatg
     7381 gggtaactta tcgtgatgaa gttattggca ctcgcccatt tgtcgtcaat ggacatacaa
     7441 ttaaagacgt tgctccaagg gacagcctta cgcttaccgg catcttgcaa aaatccagta
     7501 acatcggggt cagtaaatta gccctcagaa tgccggcaaa tgcgttagtg gatacttaca
     7561 cgaaagtcgg tttcggtaaa gacaccggtt taggcttggg tgaacaacga ggtacgaacg
     7621 gtgatcgcaa acgttgggcg gatattgaac gtgctaccct ttcctatgga tacggtttaa
     7681 atgtgacccc attacaactt gcccgtgctt atgcgacttt aggaagtttc gggctgtatc
     7741 gcccactttc gattaccaag gtcgatccgc cggtgattgg cgaacgggtg ctaccggaaa
     7801 aaaccacccg agatgtcgtg catatgatgg aaagcgttgc ggctaaaggc gaaggcggac
     7861 accgtgcagc ggtggacggc taccgtgtag ccatcaaaac cggtacaata cacaaattag
     7921 aaaaaggtaa atatgttgaa aaatatattg cttataccgc agggcttgcc ccggcaagcg
     7981 atcctcgctt tgcattagtg gtattaatca acgaacctaa agcaggcaac tattatggtg
     8041 gctttgtttc tgcaccactg ttctcacgca ttatgggtta caccttaaaa caacgtaata
     8101 ttaagccgga taacttaacg gaaaataccc aaagtgccgt gcgtgaaatt aaattagaac
     8161 gccctatcaa ttaaggttag ctatgaaacg tttactccct tttctgacag aacttgatgc
     8221 ttgggtggaa gaactcaccg agctaaaaca aatgacactt gatagtcgcc aagttcaagc
     8281 cggtgatctc tttgttgctt taaaaggaca tcaagtcgat ggcagaaaat ttattccgaa
     8341 tgcgatagaa caaggagcag caattatttt ggctgaggct gatgaagatc aaccggaaat
     8401 tgagcttgat ccgaaatttg caaaatttaa cctagatcgc accgcttgtt gcaaggttgt
     8461 ctctgttcca cgtctgccaa aattgctctc tgaaattgca ggagctcttt accataaccc
     8521 gtcacacaag ctgactttag ccggcattac cggcacgaac ggtaaaacga ctactgctca
     8581 attattagca caatggcata atttattggg tgggaaatcg gcagtaatgg gtactatcgg
     8641 caacggtttg tacggacaag tgcaagaagc ggtaaatacc accggctctg ccattgaaat
     8701 tcagcataat cttgcgaact ttgttgaact tggagcggat ttttgtgcga tggaagtcag
     8761 ctctcacggt ttggctcaat ttagagccga agccttggat tttgatttag cgatctttac
     8821 gaatttaagc cgagatcacc ttgattacca caacacgatg gaagagtatg cccaagccaa
     8881 attccgtctc ttcaacgaat taagcaccaa agctcaagta attaacgccg atgatgaaat
     8941 cgggcgtgaa tggctgacac aattaccaaa tgcagttgcg gtaagcaccg atccgaagtt
     9001 tgcaggcaac caccaatttg tcaaagccac tgcggtaaaa tttaccctac aaggggcaag
     9061 tattgcgttt gaatcaagtt ggggcaatgg cgagttacat agccgtttaa tcggggcttt
     9121 caatgtaaat aatctgctta ccgcctttgc cggactttta gctcttggct ttgatattca
     9181 agaactggtt aaaaccgccc cgaatttagt tggcgtagca ggtcgaatgg aatgcattac
     9241 cgctccaaat aagccaatgg taattgtgga ctatgctcac acccctgatg ctcttgaaaa
     9301 agccctacaa gcggctcgtt tacattgcga aggcgaactt tggtgcattt tcggctgtgg
     9361 tggagaccgt gacgccggca aacgcccatt aatggcgacg attgccgaga aattaaccga
     9421 taaagtgatt gctacagacg acaaccctcg caccgaagat aatcaaaaga ttatggcaga
     9481 tattgtgaaa ggttttagta aaccccaaca aattatccac aatcgtgaag aggcaattaa
     9541 aaccgccatt gagcaagcta aagccacaga tgtgattcta atcgcaggca aaggtcacga
     9601 agattatcag attattggca cagagaagtt acatttttcg gatcaggaaa cggcaaggaa
     9661 atatctgttt taatgtcact ttctttgcac aagcaaagaa aagtgacagt gacaaataca
     9721 aaatgctcgt aattaatttt ataaagaaaa agataaatca atgataaaac taaccaccca
     9781 acaaatcgct gaaatcctaa atgccaaatt gatcggcaat ggcgaaactg tggtagaaac
     9841 caccagcacc gacacccgac aagcggttga aaacggctta ttttttgcat taaagggcga
     9901 gaactttgat gctcatcact atcttcaaaa tgcagtcgca caaggttgtg tagcggttgt
     9961 ggttgagcgt gagtgtgaaa ttgatgtacc acaacttgtg gtggcagata cccgtttggc
    10021 attgggggaa ttggcgaaat ggttaaaagc taagctgaac ccgaaaacgg tagcgatgac
    10081 aggttcttcc ggcaaaacaa ccgtcaaaga gatgacggca aaaattctgc aaaaaatgac
    10141 cgcttgcgaa caggaagtgc tttatacttt tggtaactta aacaatgact taggtgtacc
    10201 gatgaccttg cttcgcttaa ccgaacagca taaatatgcg gtcattgagc tgggggcaaa
    10261 tcatatcggt gaaatcgctt ataccaccgc tatcgctcag cctgatgcct gcttggtgaa
    10321 taatgtggct gcggcgcatt tagaggggtt tggttcatta gcaggcgtgg cacaagccaa
    10381 gggcgaaatt tatcgtggct tgaaagaaaa tggcaaagcg attgtcaatt tagcttttta
    10441 ttatccgcaa tggcaaaaag agattggctc acgggaaatg caatctttct cttatatcgg
    10501 gtcagattcg gattcttacg cagattactg ggcggaagat gtagaactgc atttaagcgg
    10561 ttcacgcttt acgctacata ccccacaggg cgaaattgct attgatctgc cttatttggg
    10621 caaacataac gtgagcaatg ccttagcggc aacttcgctg gcgatgtctg ttggggcaac
    10681 gctagaagca gttaaggcag ggcttgagca gcgttcaatg gtaaaagggc gtttgtatcc
    10741 ggtgcaaatt aacgaccact gtttgtttat tgatgacact tacaacgcca atgtggattc
    10801 aatgaaatcg gcgatttctg ttttggaaaa ttatccggct tatcgtattt ttgcagtggg
    10861 tgatatggcg gaattgggtg aagaaagtag caaatgccac caagaagtag cggattttgc
    10921 caaaaatgca gcattagatt taatcgtgag ctttggaaaa gaaagtgcgg taatcagcca
    10981 acaggcagaa catcatttta ccgataaaca gcaaatggtt gatttcctac tgccgattat
    11041 tttgcaaaaa atcgaacaaa aacaaccgct tgtattattg gcaaaaggct ctcgtagcca
    11101 gaaaatggaa accgtgattt ccgaattgtg taaaccgttt aacctaaatt tctaattatt
    11161 tttaaggact aagcaatgtt agtttggctt gcagaactat tggttcaata taacacagcg
    11221 tttaacgttg tctcttatat tactttccgt gccattatgg cgttattaac tgcaatggga
    11281 atcgggcttt ggatcgggcc gaaagtaatt cgccgtttgc agatcttaaa atttggtcag
    11341 gaagtgcgta atgacggacc ggaaagccat ttcaaaaaac gtggtacgcc aacaatgggc
    11401 ggagtgatga ttctcgcagc gattggggtg agtacattgc tttgggcaga tctgcgtaat
    11461 tcctatgttt ggttcacgct gtttgtgcta tttggctacg gtgcagtggg ctttgttgat
    11521 gattatcgta agatctctcg taaaaatacc gatggtttga ttgcccgttg gaaatatttt
    11581 tggttatctg ctatcgcatt agtggcggtg tttggtatgt atgcggtggg taaagatacc
    11641 gctgcaacac aattagtcgt gccgtttttt aaagatgtga tgccgcaact cggtttattt
    11701 tatattgtgt tatcctattt cgtgattgtc ggcaccagta atgcggtaaa cctaacggac
    11761 ggcttagacg ggcttgcgat tgtgccaact attatggtcg catctgcctt tgcgttaatt
    11821 gcgtgggcaa ccggtaactt taattttgca caatatttac atattccttt tattccaaat
    11881 gccggtgaat tggttatttt atgtactgcg attgtgggag caggtttagg gttcttatgg
    11941 tataacacct accctgcaca ggtgtttatg ggcgatgttg gctcactttc gctcggcggt
    12001 gcattaggcg tgattgcagt attagttcgc caagaattat tattggtggt aatgggcggt
    12061 gtatttgtgg ttgaagccct ctcggtgatt ttacaagtag gttcatacaa attacgccaa
    12121 aaacgtattt tccgaatggc tccaattcac caccattttg aactcaaagg ctggtctgaa
    12181 ccacgggtta tcgtgcgttt ctggattatc acgctaatgc tggttttagt cggcttagta
    12241 acattgaaat tacgttaatt gattgaataa ggtgcattga gataatgtac cttatgttat
    12301 ttttaaatta cagaataaat tatgcaaaaa caatacgaaa acaaaaccgt tactattatc
    12361 ggcttaggta caacagggtt atcttgtgtg gatttcttta agaaaaaaaa tgccaatatc
    12421 caagtgattg acacccgagc aaacccagcc ggagcggata aattggacgt aactattccg
    12481 ttgcataccg gtgggttaaa cagtgaatgg ttgctgaaat ctgatcttat tgtaattagc
    12541 ccaggtttag ccgttgccac acctgaaatt cagcaagcga ttcaagctgg tgttgaaata
    12601 gtcggtgatg ttgaactgtt ttgtcgggaa gcgaaagctc cgattattgc gatcaccggt
    12661 tcaaacggca aaagtaccgt aacgacttta actaccgaaa tggcgaagca agcggggatt
    12721 aaagtgggaa tgggtggcaa catcggtgtg ccggtattaa gtttgctgga gggcgattat
    12781 gacctttttg tgctggagct atccagcttc cagcttgaga caacttattc actgaaagca
    12841 aaagctgcca ctgttttaaa tattagcgaa gatcatatgg atcgctacga ttcgattgcc
    12901 gactatcgcc aagcgaaatt gcggatttac gataatgcgg aaaatatcat cgtaaacggt
    12961 gaagatgccc aaacttaccc gatacaagcg gtcaaaaaag tgattcgttt tgcagaacaa
    13021 aatgcggaat atagcctacg caatcagatg ctttattcag gcgatgatgc aattatccaa
    13081 actaaacaga tgctgattag cggccgtcat aatgagatga atgcgttagc ggcgatggca
    13141 ttagcagaag cggcgaatgt tcctcgtgaa ggcattgtga aagctttgca aatttatggt
    13201 ggtttgccac accgtttcca agcggtggca accaatgatg gcgtgcgttg ggtcaatgat
    13261 tccaaagcga ccaatgtggg tagcacggta gcggcattaa acggcttgaa tattgccggt
    13321 acactttatt tacttttagg tggtgacggc aaggctgcgg atttttccga actcaaacca
    13381 ttaattaata agccaaatat tgtctgctat tgtttcgggc aagatggagc ggcattggca
    13441 gaattaagcg aacaaagtgt gttggttgaa acgatgcagc aagcaattga agcaatccgc
    13501 ccacaactga aaaaaggcga tatggtactg ctttctccgg cttgtgccag tttagatcag
    13561 tttaaaaact ttgagcaacg tggcgaggta tttatggcat tagcacaagg acagcacgca
    13621 taatgttaga aagaattaag tcggaatggg ataaatgggt cagattaacc ccttctaatt
    13681 cactttatga cagaactcta atttggctat ttttaggcct gctaattatc ggttttgtga
    13741 tggtaacatc tgcttcactc ccagttagca caaggttgaa taacgatcca ttttattttg
    13801 cgattcgaga cgggctgtat attattgctt caattatctt ttgttatgtt tttgtacaaa
    13861 tcccaattga aaaatgggaa aagcataact tggcactgtt ttttatttca atcggttttt
    13921 taatcgccgt actcattttc ggccgctcaa ttaatggtgc agtgcgttgg attccgttgg
    13981 gtattctcaa ctttcagccg gcagaattag ccaaattagc ggtcatttgc tattttgcca
    14041 gtttctatgt acgtaaatat gatgaaattc gtaaagaaaa agccagtttc tggcgaccgg
    14101 cagtgatttt attcttattc ggcttcttgc tgattttgca gccggattta ggtagtacct
    14161 ttgtattatt tgttctcact ttctcaatgc ttttcattgt gggggcaaaa attatgcaat
    14221 ttatgttttt aggcgtggtg ggaacggtgt tatttgcggt gttgatttta acctcggaat
    14281 accgtttaaa acgtgttacc tcctttatgg atccgtttgc tgatgcttat ggcgacggtt
    14341 tccagctctc gaactcacaa atggcatttg gacaaggtga attttgggga caaggtttag
    14401 gtaattcggt acaaaagcta gaatatttgc cagaggcaca tactgacttt gtaatggcgg
    14461 ttattggtga agaatttggt ttctttggta ttgcttgcat cgtactttta cttattctat
    14521 taacggtgag agccttaaaa atcagtaaag aatctttggt gctggaagag cgttttaaag
    14581 gctatatggc atttgggatt gcgatttggg ttttcctaca aggcttcgta aacttagggg
    14641 tggcatcagg tttgctgcca acgaagggct taaccttccc attagtgagt tatggtggtt
    14701 cgagtttggt aattatgtcg attgcgattg cagttttgct acgaattgac cacgaaaacc
    14761 gtgccgagcg aatcgggcac gcaaaaatta aaaccatcta gccgagcggt ctgcaacgtt
    14821 acaatttgca aaattttaca aaaatgagac cgcttgtatt agctggaaag cataaaaatt
    14881 cagtaaaata gcacgtttta gaataaaaaa gagttgaaaa tggcgaagaa attattagta
    14941 atggcaggtg gaacaggggg gcacgttttc cctgcaattg ccgtagctcg agagctacaa
    15001 aaacaaggct ggcaaatccg ctggctgggg acgaaagacc gaatggaagc cactcttgtg
    15061 ccgaaacacg gtattgagat tgattttatt gaaatctcag gcttgcgggg taaaggcgtt
    15121 gcagcattgt tgaaagcacc gtttgctatt tttaaagcgg tgatgcaagc ccgcaaaatc
    15181 attaaaaatt atcagcctga tgcggtgttg ggaatgggcg gttatgtgtc cggtccgggc
    15241 ggtattgcgg ctaaattatg tggcgtgccg gtgattttac acgagcaaaa cgcagttgcc
    15301 ggcctaacca atgtgtggct atccaaaatt gcccgcaagg tgttacaggc tttccctact
    15361 gcttttaaag aggccgaagt agtaggtaat cctgtacgtg aagatttatc tgctcttccg
    15421 ctgccgaatg aacgtttcgc caaacgaggc tacccgatta acattttagt aatgggtggc
    15481 agccaagggg caagagtgat caaccaaacc gtgccggaag tggcaaaagt gttgggtaat
    15541 aatgtgttta tcagccacca agtcggtaaa ggtaatttag ccggtatcga agaagtctat
    15601 caacaaaccg gcaatgggat tgcttccgag tttattgatg atatgaaagc cgcgtatgag
    15661 tgggcagatt tggtgatttg tcgttccggt gcgttaacgg tgtgtgaaat tgccgccgta
    15721 ggtctgcccg ctgtgtttgt gccgttccaa cataaagaca ggcaacaatt tttaaacgct
    15781 aactatttgg cggcagatgg agcagcagtg attatcgaac agagcgattt cactccaaag
    15841 gcattacagc aagccttaga acccttaatt gccaatcgcc aactcttatt ggatatgtca
    15901 atgcgagcca gagcaaaatc caccccaatg gcagcaaaac gtgtagcgga agtgattatt
    15961 caagaagcga attaacttac tacaagcggt cgtttttggt aaaaattttg caaaatgtaa
    16021 ccgcttgcga atagattaaa aggacaataa atgaaaaatt tccaagataa agtcaaaaaa
    16081 ttagtgcctg aaatgcgtcg tgtcagccaa attcatttta tcgggattgg cggagccggt
    16141 atgagcggca ttgcggaagt gttattaaac gagggatacc aaatttcagg ttcggatatt
    16201 gcagacagag cggttacaca acgtctatcc gcagccggag cgaaagtctt tatcggacac
    16261 caagcagaaa atattgctgg ggcgagtgtg gtggtggttt ctactgcgat tgatgaaacc
    16321 aaccctgaaa ttattgccgc tcgagaagct cgaattccgg tgattcgccg agcagaaatg
    16381 cttgccgaaa ttatgcgttt tcgccacggc attgcgattg caggcacaca cggtaaaacc
    16441 actacaaccg caatgatttc aatgatctac accgaagcaa aattagatcc gactttcgtg
    16501 aatggcggtt tggtaaaatc taccggcaaa aatgctcatt tgggggcgag ccgttattta
    16561 attgccgaag ccgatgagag tgatgcttct ttcttgcact tgcagccaat ggtgtcggta
    16621 gtaaccaaca ttgagcctga ccatatggac acctatggtg gcgattttga gaaaatgaaa
    16681 gagacttacg tacgattttt gcgtaactta cctttctacg gcttagcggt aatgtgtgcc
    16741 gatgatgaaa cggtaatgga aattgctcct cgtgtcggca gacaagtgat tacctatggc
    16801 tttagcgaaa atgcggatta ccgaattgaa gattaccagc aaaccggctt ccaagagcat
    16861 tacaccgtag tttgcccaag cggtgaacga attgatgtgc tgttgaatgt gccgggtatg
    16921 cacaatgcct taaatgcaac cgctgcgtta gcggtggcaa aagaagaagg gattggcaat
    16981 gaggcgattt tagctgcttt agctgatttc caaggggcag gacgccgctt tgaccaatta
    17041 ggttcattta ttcgcccgaa cggcaaaatt atgttagttg atgactacgg ccaccaccca
    17101 actgaagttg atgtaaccat tcaagccgct cgttcaggct gggaaggtaa acgtgttgtg
    17161 atgattttcc aaccgcaccg ctattctcgt acccgtgatt tattcgatga atttgtacaa
    17221 gtactgtcta aagtcgatgc cttgatttta ttggatgtgt atgctgccgg cgaagctccg
    17281 attgtggggg cagacagtaa agccttagcc cgttcaatcc gtaatttagg taaggtcgat
    17341 ccgattttgg tttcagatac cgatcaactt ggcgaggtat tagatcaaat tattcaagac
    17401 ggtgatttaa ttttggcaca aggtgcgggc agtgtaagcc gcatttctcg tggcttggct
    17461 gagagctgga aaaattaaat actctctccc cttgcgggag agagaccgaa aaatacttcg
    17521 ttcagaagta tttttcagag agagggtata tatttaaagg atttcaaatg agtattaaaa
    17581 acgaaaaaat tgccgtctta ttcggtggcg tttcgcaaga gcgtgaagtt tcactgaatt
    17641 ctggtgcagc ggtaacagct gcattaaaaa gtttaggcta taacgtagaa ggcattgata
    17701 ctaaagaatt cccgattgaa aaattaaaag aaaaagggat tcagcgtgta tttaatattt
    17761 tacacggcgg tattggcgaa aatggcgtac tgcaaggtgc gttagagcaa atgggtattc
    17821 cttacaccgg ttgtggcgta atggcatcag cagtgacgct ggataaattc cggactaaat
    17881 tattatggaa tgcagtaggt ttaccaacag cggatatggt ggtggttcag cgtggacaag
    17941 cggtcgattt tgaccaaatt attgcaaaat taggcttacc ggtatttgtg aagtcttcgt
    18001 gtgaaggctc aagtgtgggt gtattcaaag taaaaaccaa agaagaattg gcaccggcaa
    18061 ttcacgaggc attaaagttt gattccattg tgttggtgga agaattttta gccggtaatg
    18121 aatattctgt accggtatta gacggacaag ttctaccggc ggtacaagta attccggacg
    18181 gcgaatttta tgactaccac gcaaaatatg tgtcggataa cacccaatat gtagtaccgg
    18241 cattaaatga cgaacgccaa gcagaggtcg ccaaattagt gaaagcggct tacgatgtgg
    18301 ttggctgtcg aggttggagc cgtattgatg tgatggaaga tggcgaagga aaattccgct
    18361 tggttgaagt aaataccaac ccgggaatga ccagtcacag tattttccca aaatcagcag
    18421 caacagttgg tatttccttt gaaaaattag ttgaacgcgt attggagtta agtgcttaat
    18481 gggcatcttc cgcaagaaga acagcacaaa tgtgcgtttg aatgttctta atccaaaaac
    18541 acctgtttct ccacgtaaat ggctagtatt cattaagcca ttaattgtgt tattatgtac
    18601 actatttgta ttcggtgtgt atagtaattg gcaaaatata ctcgaaagtt gggataaaac
    18661 accgattcgc gcttatgcat taacacataa aacacaattt acaacaaatg cagatattcg
    18721 tgaaactttg tctaaagaac ctgctctaaa aggatatttc ggacaaaata ttcaagaggt
    18781 caaagataag cttttatcta taccttgggt taaagatgtg gctgttcgta aactttatcc
    18841 cgaccgatta agtattactt tgctagaaca taaacccgtt gcaatttgga atgatactaa
    18901 ctttgtctca gaacaaggaa ccgtctttag tttaccgaaa gacagaatcg ataaaaatgg
    18961 acttccatta ctctatggtc cagatacgga aggaaaaagt gtattagagg catggaataa
    19021 aatccgagct gatctaaatg cccgcaactt ggatttaacc tccgtttctg tagataatcg
    19081 aggttcttgg acaattaggc tttctaatca agtagaatta agattaggaa gaggggaatg
    19141 gacttctaaa attgatcgtt ttgtaataat ctttcctgaa attcatatac ctgaaggtaa
    19201 tagattagct tatgttgatt tacgttatcc acatggcgcg gcggttggat ttagtcctat
    19261 aaaaaattaa actcagtgaa cacaaaaaca ttatgacaaa agcagtagaa tcaaaaatta
    19321 tcgttggtct tgacattggg acatcaaagg ttgttgccgt tgttggtgaa gtattacctg
    19381 atggtgtaat taatgttatg ggagctggcg tttgcccttc taaaggagtc gatcgaggtg
    19441 gcgttattga tcttgacgct gccgttaatt ctattcaaag agccattgag caggcggaat
    19501 ctattgccga ttgtcaaatt atgggtgtaa cactggcaat ctcaggtcag catattgttg
    19561 gtttaaatga agcaggatca tcaacattat ctggaggtat tgttacccaa gataatattg
    19621 aaagtgctgt ggatatggca aaggcaatta aattacaaga tggcttagaa acattacaca
    19681 tgattccaca agaatatcac gtggatagat tgcctgcgac taaaaatcca attggcttat
    19741 cgggtatgcg tttacaagtt caagcacact taattgcttg ccatcaagct tggttaaaaa
    19801 acttaaaaaa tgcggttgaa catgctaaat taaaagtcga tcaagttgta ttttcaggtc
    19861 ttgcctctag ctattctgtt ttaactgaag atgaaaaaga gctgggtgtt tgcttaattg
    19921 atattggtgg cggtacgatg gatgtactag tttataccga tggagcatta agatacagta
    19981 aagtcattcc atttgcaggt aataacatta ctgattattt ggctcgtgta tttacgacat
    20041 ctcgccctga agctgaaagc cttaaagtag gttatggtag cgcaatttcg cctccaaccc
    20101 ataattcaga taaaaaaatt gaggtggcag gactaggtgg acggatggca agaaccttta
    20161 cccgagctca ggtggcaacg gttacctcac aatgttataa cgatttgcta aaagttgttg
    20221 aagaggaatt aacccaatta cgccatgaac ttttccaaaa aggaataaaa caagagttaa
    20281 ttgccggtgt agtcattact ggtggtggct cacaaattga agatattgta gaatgtgcta
    20341 aatcaatttt tggttcacaa gttcgtgttg gatatccact caatattact ggcttaaccg
    20401 attatgtgaa taaaccacaa tatgcaacag cattagggct tttacaatat agccactata
    20461 attatactga acaaggtaca agtacagatt ctaatgaaga tatctttgct cctattggtc
    20521 gtggaaccaa gaaaatcatt gacttcttca aatcaaaatt ttaatagtgt taaaataaac
    20581 acgcagggag ataaaaatgt ttgaacctat tattgaacaa acgcaagatg ctatcattaa
    20641 ggtagtgggt gttggtggcg gtggcggtaa tgccgtagac agaatgtctc gctctgccga
    20701 tgatattaaa ggtgttgaat tctttgatgt aaatactgat gctcaagttt taagaaaaag
    20761 aaccacacgc caaactatcc aaattggagc aagtacaact aaaggtttag gtgcaggtgc
    20821 tgatccaatg gtaggaaaac aagctgccga agaagatcgt gaggctatcg ctaatgcatt
    20881 aaaaggtgca aatatgacct ttattgctgt tggtatgggt ggaggtaccg gtaccggagc
    20941 agcccctgtt gttgctcaaa ttgccaaaga gcaaggctca ctaactgtag gcgttgtaac
    21001 aaaacctttc cgcttcgaag ggcctcgccg tatgcgcttt gcagaccaag gcattaaaga
    21061 gctttctcaa tatgttgatt ctcttattat aattcctaat gataaattac gtggtttagg
    21121 taagcaaaca acagcagtag atgcctttgc cgcagcaaat gatgttttaa gtaactgtgt
    21181 gcttggtatt accaatatga ttaccagttc gggcggttct accggtgcag atattaacgt
    21241 ggacttcgcg gatgttcgta cagtaatgtc tggtaaggga cacgctatga ttggtacagg
    21301 ttttgctgaa ggtgaagttg gtgaaggacg agcggaaaaa gcaatgaatg atgccatttc
    21361 tagtccatta cttgaaaatg tggatatttc aggtgcaagt ggtatgctca tcaacattag
    21421 tgctgggaca gatttcttat tagaagaagt ttatgctatg atggacttaa tttatggctt
    21481 tgcaacagaa gatgccgcaa ttgtatttgg ttgtaactat taccctgaaa tggatggaaa
    21541 agtgagtgtc actcttgtcg caacaggaat aggccaacct gaggaagctc tacatatgcc
    21601 acataaggct cagccagtat atgcacagca aggaaatcat gttcaagcta cacagcctac
    21661 tcagccaaac catgcggtac aacaaccaaa ctttactcaa ccgtcaagtt atggtcagcc
    21721 aaatcaattt agtggtcaac cacaaccact acaacaacct gtggctccaa aaccacaatc
    21781 ggttgataca gcacaaattt ttaacccaaa ttcaattccg ggttttatga gaaatagtca
    21841 ataacttcca ctcatttcgg aagtaaggac aaaatatgac taaacaaaga acattaaaac
    21901 aagcagcaaa agtaacaggt attggcttac atagcggcaa aaaagtaacc ttaacgttac
    21961 gccctgcccc agctaataca ggagttattt atgcccgtac cgatttagag cctgtcgttt
    22021 atttccctgc aagcgcagac tctattcgtg atacacaatt atgcacatgt atggtaaatg
    22081 atgatggtgt tcgtatttca acagtagaac atttaaacgc agcaatgtca gcattaggct
    22141 tagataatgt tattgttgaa gtagatgcac ctgaaattcc gattatggac ggtagttcaa
    22201 gcccatttat ctatctttta ttagatgccg gcatcgaaga acaagatgca ccgaagaaat
    22261 ttattcgcat taaagaaaca gtacgcgttg aagaaggcga taaatgggcc gaattaaaac
    22321 catacaataa aggttttaaa ttgaacttca ctattgactt tgagcaccca ttaatcccca
    22381 aaaatgtagg gagctattcc attgattttt ctgctgagaa ctttattcag caactaagtc
    22441 gagcgagaac ctttacattt atgaaggatg tagaatactt gcaatctatt ggtcttgcat
    22501 taggtggaag ccttgataat gctattgtat tagatgatta ccgaattcta aatgaagacg
    22561 gtttacgatt taaagatgaa ctggttcgcc ataaaatgct ggattcgata ggtgacttat
    22621 atatgtgtgg ttataatatc ctaggtgaat ttagtgcata taaatcaggt catggcgtta
    22681 ataataaatt attaagagct gtattggaaa ataaaaacgc ttgggagttt gttacctttg
    22741 aagataaagc agaagtccca caaggttacc aagtacaaca agaaattttt atttagtcta
    22801 aatggcttaa aagacgaaca gaaatgttcg tcttttattt tgcaaatttt ttctaatatt
    22861 taaccgcttg caacccgcct cattagcgat tttttcgccc ttatctattt actgtttaaa
    22921 aaaacagttg tactatatac ccatacagtt aatcttgtgt ggtgtattat gcttactcaa
    22981 cttactatta ataattttgc gattgttcgc catcttattc ttgaattaaa tgaaggtatg
    23041 tctgttatta ccggtgaaac cggtgccggg aaatcaatcg ctattgatgc cttagggcta
    23101 tgcttgggtt atcgttctga aagtgggatg attagaaacg gttcggacaa agcagatata
    23161 agtgcaacct ttactatgca gccacatagc cctgcttatt tatggctaca agagcatgaa
    23221 ctgcttgatg aagataatcc tcaagagtgt atcttacgcc gtatgattaa tattgatgga
    23281 cgctcaaaag cctttgtaaa taaccgatca ttacctgttt cacaacttcg agagttgggg
    23341 caatatctta ttcatttaaa tgggcaacac gcaccacaat tactcctaaa aaacgaatat
    23401 caacttgaaa tagtcgatca ttatgccacg ttacagcctc tattgatgaa gatggcaaag
    23461 caatatggga attggaaaga actgaataag caggtaaaaa atttccaaaa acagtgccaa
    23521 gaaaatgaag ctcgcaaaca gctattagaa tatcaagttg aggaattaga tgaatttgca
    23581 attaaagcaa acgaatttga agaaatggaa gagaaataca atatcttgtc ttcttccgag
    23641 gagcttactg agctttcaga tgaaatcaca cgcttattaa gtgatgatga aatcaatgca
    23701 aacagtctga tttataaagc gttacgcaat gcagaaaact tggctgaggt tgatagccaa
    23761 tatcagggca ttttagatat gcttaatgag gccctgattc agattcaaga agcaagctcc
    23821 gaagtggctc aattagccag taaaattgaa caagatccgg aattactaca ggagctggat
    23881 aatagaattg cacaaactat atcgttagca cgcaaacacc atattcaacc gaatgaatta
    23941 tggcaatatc acgcgacatt aaaagcagaa ctacaggaat tactggattt tgaagataat
    24001 gaagaacaac tcattcaaaa tgaaaaacaa gcttaccaag agtgtataac attagcagaa
    24061 gaaatttacc aaaaacgtct agatgccaca cataagctct ctgaacaagt aacaaaacaa
    24121 attaaacact tagcaatgga aaacggtgag ttctttattc atatagaaca taatcttgaa
    24181 aaaatgtctg caaatggtgc tgactatgtc gagtttaatt tacgcagcaa tttaggacaa
    24241 caaccccaac cactggcaaa aattgcttct ggtggtgagc tatctcgcat ttctctggca
    24301 gtacaagtgc ttaccgcaaa taaactttct acgccaacta ttatttttga tgaagttgat
    24361 gttgggatta gtggctctgc tgccactaca gtcggaaagc tactgcgtaa gctaggcgaa
    24421 aaatgccaag tattatgtgt aacccattta cctcaagtgg caagttacgg caaccagcag
    24481 tttaatgtac aaaaatatgt agaaaataat caaactgaaa cgcaaatgac attactcaca
    24541 gaagcagagc gtattcacgc cttggctcga ctattaggcg gaagcgaaat caccgaaacg
    24601 gttttagcta atgcaaaaga aatgctaaat ttagcgaaaa ataacgaata gccaaaaaga
    24661 aaaatcgcat caatgaatat tgatgcgatt atgaatttgg tggaggtaaa cgggatcgaa
    24721 ccgatgacct cttgcatgcc atgcaagcgc tctcccaact gagctatacc cccttaatac
    24781 aagcggaata atatgctttc cgcttgaaga tgtcaatggc tttaatcaag caaggtgttg
    24841 attgattaaa aactagctat tcgcctgaat aaactcaatc gctttttgta agcgagctaa
    24901 ggttctttct ttaccgacta atttggcggt gatatccatt gacggcgatt gcccacttcc
    24961 tgttaccgct aaacggaacg gcataccgac tttgcccata ccaatttcca gctcggcagc
    25021 ggtttggttc attgcctcgt ggatattttc caccgtccag tcattgcaag cggtcaattt
    25081 ttccaataat tttgcaagtg gagcaacggc ttcggctttg aagtttttag cagcagcttt
    25141 ttcgtcgtaa gccttaaatt cttgatagaa atagcggctg ctttcggcaa gctcttttaa
    25201 ggttttgcaa cgttcgccca gtacagggat aatttcttcc aattttggac cgcttgtgaa
    25261 atcaatgcct tgagtttgca tatgccattc aagatgtttt gccacataac ttggggcaag
    25321 actgcggata tagtgttggt tcaaccattg taatttttca gtgttaaatg cacttgccga
    25381 tttactgact gaatgaatgt cgaacagctc gatcatttcg tcacgagaga aaatctcttg
    25441 gtcgccgtgt ccccagccta aacgcacaag gtagttaatt aaggcttccg gtagatagcc
    25501 gtcatcacga tattgcatta cgctcaccgc accgtggcgt ttggagagtt tttgcccgtc
    25561 atcgccgttg atcattgaaa cgtgggcgta ggttgggatt ggtgctccta atgctttcaa
    25621 aatgttgatt tggcgtggtg tattgttgat atggtcttca ccccgcacaa cgtgagtaat
    25681 gcccatatcc cagtcgtcca ccaccacaca gaaattgtag gtcggcgagc cgtcagtacg
    25741 gcgaataatc aagtcatcta attcgctatt ggcgatttca attcgcccac gcaccgcatc
    25801 gtcaaatact accgagcctt cggtcgggtt tttgaaacgc acaacatgtg gttggctcgg
    25861 gtcgttttgc ggattgtgta agcagtgctg atcgtatttc gctttctctt tatttgcttc
    25921 ttgctctgca cgtacttcgt ctaaacgttc tttcgagcag tagcaacggt atgccgtgcc
    25981 ggcttctaac atttggtcga tcacttggtt atagcgatcg aaacgcttgg tttgaaagta
    26041 tgggccgtgt tcccacgcca aatccagcca ttccataccc tctaaaatag cggcggttgc
    26101 ctcaggggtt gagcgttcaa gatcggtatc ttcaatacgt aacacaaatt caccatcatt
    26161 atgcttagca tataaccaag agtagagcgc ggtcctcgcc ccacctacgt gcaagtagcc
    26221 agttgggcta ggtgcaaaac gggtacggac ttttacattc ggatctaagg ggaaaagggt
    26281 ttcaattttc attttatatt gcctaattga tgattcttaa gaaaaacttg tctattttac
    26341 tacgcttgag ggtgaatcat agtaaaaaat taaatttgcg gggtaaaaaa tcagcaaacg
    26401 gggtaaaaag aacaaaaagg gtttgacgaa acagcaaaaa tctctataat tcacttccac
    26461 aacagcgggc gattagctca gttgggagag cacctccctt acaaggaggg ggtcactggt
    26521 tcgagaccgg tatcgcccac cacttcaact taagtgtttc gttgttgtat cacaatctaa
    26581 ttttgaaaac gggcgattag ctcagttggg agagcacctc ccttacaagg agggggtcac
    26641 tggttcgaga ccggtatcgc ccaccacttc aacttaagtg tttcgttttc aaattccctt
    26701 tgattttaag tctcaagtgg gcgattagct cagttgggag agcacctccc ttacaaggag
    26761 ggggtcactg gttcgagacc ggtatcgccc accacttaag actaaaatca taaacttcaa
    26821 atggcgattt ttcgtatctg aagggcgatt agctcagttg ggagagcacc tcccttacaa
    26881 ggagggggtc actggttcga gaccggtatc gcccaccatc tatttagtat caaagcctaa
    26941 aacttatatc ataatctaat aaccccttta aagtcataac taaaaaggga aaataaattc
    27001 agatggcctg ttgcatctgc ataaatgcct ttctacgtgc agaaattaat ctttttctat
    27061 ctttaatttg cttaatttga cgcaggcgta atttccttgt cagtagggat ttctttttct
    27121 tcaattttct cataagaaga attctcctgt tttatttgac catctggtaa attttcttgt
    27181 tcttgcatct tttgctcttc aactcgttga taattaataa tactaccata ataacgccgt
    27241 atattttcaa catactgtaa tgcttcatat cctcgagcat agccgtattt tagattagca
    27301 taataacgtt tttccgcaag caatggcaga ttctttttaa catcaagcca attatccgga
    27361 tcaccaccaa gttgttttgt taatcgacga agatccaaca agtgccccaa tcccatatta
    27421 tacgctgcta ggctatacca aattctatct gcttgaggaa tagtttccgg catttgtctc
    27481 attagcatat gtaaatattc agatcccgct ttgatacttt gtgcagggtt agtcctatca
    27541 gaaattttca ttctatctgc cgtatctttt gtcagcatca taattcccct cactccggtt
    27601 ggagaagttg cattaggatc ccaatgagac tcctgatagg cgatagccgc cagcatttgc
    27661 cattctaatt cacctttatg tgcccgaaaa aggggttcat atttaggtaa aatagtttta
    27721 attgcattca aatagctctg aatatcaaca taatcaaact tagctaaatg attaaaatat
    27781 ttctcttcta ttcttgatat cagcccagtt tccatagaat aagacataaa atccagcagc
    27841 acagattgca gttcactata agaactattt gctaaatacc ataacactgg gttttcatca
    27901 gttaaatcaa accctacggc taaattagga ttaatatgct gtgcagcaga aatatcaacc
    27961 gacattgcaa ttgtatgtga aatttcacct tgtgcgactt ttaacaatag ctcttcttgt
    28021 gtaaatttat ccgagatctg ccattttagt tccggattat ccaccttaag ctgatttaat
    28081 attggaatta ccgcagaacc ggctggaata atcacatttt cccgcaaatc ggaaagtcga
    28141 tatggtctag cacttccctt tttataaaca agctgccaag aagccgaata ataggaagga
    28201 cctatctgaa attcatcgtt taattcagaa tggtaaagca gcccagcggc agcaatatca
    28261 acatcattat ttcttaaggc ctcaaacaat ttctcgctac tatcataagt tttaatatca
    28321 agattgacct ttaaatattg agcaaaggct gttgctaaat catattcaat gccacttttt
    28381 ccttcaggac caacaaagta agaaagtgga tggttgatca ttccaacctt taacgttttg
    28441 ctttgctgaa tttgtgtata atgatttgct tctgaatgaa tgattttttg ccaaggaaaa
    28501 atcatatccc aagcccacag taacaatgct acggcaataa aaaggcgagc aattaatcct
    28561 ttcaatttaa aatcctcatt atttcaagct aagcattaag cctatctgaa at
//
LOCUS       DANLUA010000008        27125 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLUA010000008 DANLUA010000000
VERSION     DANLUA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27125
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            275..736
                     /gene="rimP"
                     /locus_tag="PXB44_000325"
     CDS             275..736
                     /gene="rimP"
                     /locus_tag="PXB44_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL5280843.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            757..2253
                     /gene="nusA"
                     /locus_tag="PXB44_000326"
     CDS             757..2253
                     /gene="nusA"
                     /locus_tag="PXB44_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL5280844.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            2238..4805
                     /gene="infB"
                     /locus_tag="PXB44_000327"
     CDS             2238..4805
                     /gene="infB"
                     /locus_tag="PXB44_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL5280845.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            4890..5270
                     /gene="rbfA"
                     /locus_tag="PXB44_000328"
     CDS             4890..5270
                     /gene="rbfA"
                     /locus_tag="PXB44_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL5280846.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            5273..6196
                     /gene="truB"
                     /locus_tag="PXB44_000329"
     CDS             5273..6196
                     /gene="truB"
                     /locus_tag="PXB44_000329"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL5280847.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            6199..7279
                     /locus_tag="PXB44_000330"
                     /pseudo
     CDS             6199..7279
                     /locus_tag="PXB44_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(7317..7841)
                     /gene="tadA"
                     /locus_tag="PXB44_000331"
     CDS             complement(7317..7841)
                     /gene="tadA"
                     /locus_tag="PXB44_000331"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL5280848.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(7923..9731)
                     /locus_tag="PXB44_000332"
     CDS             complement(7923..9731)
                     /locus_tag="PXB44_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL5280849.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(9933..10856)
                     /gene="nlpI"
                     /locus_tag="PXB44_000333"
     CDS             complement(9933..10856)
                     /gene="nlpI"
                     /locus_tag="PXB44_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL5280850.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(10937..13087)
                     /gene="pnp"
                     /locus_tag="PXB44_000334"
     CDS             complement(10937..13087)
                     /gene="pnp"
                     /locus_tag="PXB44_000334"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL5280851.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENI
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
     gene            complement(13349..14629)
                     /gene="phoR"
                     /locus_tag="PXB44_000335"
     CDS             complement(13349..14629)
                     /gene="phoR"
                     /locus_tag="PXB44_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL5280852.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            complement(14632..15318)
                     /gene="phoB"
                     /locus_tag="PXB44_000336"
     CDS             complement(14632..15318)
                     /gene="phoB"
                     /locus_tag="PXB44_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL5280853.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            complement(15402..16172)
                     /gene="pstB"
                     /locus_tag="PXB44_000337"
     CDS             complement(15402..16172)
                     /gene="pstB"
                     /locus_tag="PXB44_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL5280854.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            complement(16182..17030)
                     /gene="pstA"
                     /locus_tag="PXB44_000338"
     CDS             complement(16182..17030)
                     /gene="pstA"
                     /locus_tag="PXB44_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL5280855.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            complement(17027..17986)
                     /gene="pstC"
                     /locus_tag="PXB44_000339"
     CDS             complement(17027..17986)
                     /gene="pstC"
                     /locus_tag="PXB44_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL5280856.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            complement(18067..19104)
                     /gene="pstS"
                     /locus_tag="PXB44_000340"
     CDS             complement(18067..19104)
                     /gene="pstS"
                     /locus_tag="PXB44_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL5280857.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            19289..21535
                     /gene="parC"
                     /locus_tag="PXB44_000341"
     CDS             19289..21535
                     /gene="parC"
                     /locus_tag="PXB44_000341"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL5280858.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYKAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            complement(21606..22958)
                     /gene="lysC"
                     /locus_tag="PXB44_000342"
     CDS             complement(21606..22958)
                     /gene="lysC"
                     /locus_tag="PXB44_000342"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL5280859.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
     gene            23521..24951
                     /locus_tag="PXB44_000343"
     CDS             23521..24951
                     /locus_tag="PXB44_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL5280860.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
     gene            complement(25015..25344)
                     /locus_tag="PXB44_000344"
     CDS             complement(25015..25344)
                     /locus_tag="PXB44_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL5280861.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            25540..27042
                     /gene="ppx"
                     /locus_tag="PXB44_000345"
     CDS             25540..27042
                     /gene="ppx"
                     /locus_tag="PXB44_000345"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL5280862.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
BASE COUNT         7863 a         5404 c         6113 g         7745 t
ORIGIN      
        1 gggctcataa cccgaaggtc gttggttcaa atccggcccc cgcaaccagt tttaagctca
       61 cagctttggt taaagttgtg agttttgttt tatatgctgt agcaaatttg caaaaaattt
      121 ggcaaaaaag accgcttgtt gtggttgcta cagcaaaaaa caaaccgctt aaagccccac
      181 ggtttcccgt ctggggcttt tttgcggaaa ttggtacagc attaacgtat caaagctggt
      241 ttatccggct ttttttatat ttggagatta tgacttggca actttagaac aaaaattaga
      301 agatttagtg ctcgattcta ttgaagcatt aggctttgaa ttagtcggca ttgaatgcca
      361 acgtgcaggc cgttttttaa cggtgcgttt atatattgat aaagaaggtg gcgtgacgat
      421 tgatgattgt agcgatgtca gccgccaagt gagtgcgatt tttgatgtgg aagatccgat
      481 tgccgataaa tataacttag aggtttcttc accagggtta gatcgtccat tatttaccgt
      541 tgcccactat gagcgtttta tcgggcaaga tgtagtgatt catttacgca ttccgatgtt
      601 cgatcgccgt aaatggcaag gtaaaatcaa acaagtagaa ggtgatttca tcactttaac
      661 ggtagaaaat gaagatcgcc agttcgcatt tggcaatatt caaaaagcaa atttagttcc
      721 ggtttttaat ttttaagata aaggtaaact gacaaaatga gtaaagagat tttattagct
      781 gcggaagcag tttcaaacga aaagcaatta ccaaaagagg caatttttga agccttagaa
      841 accgctttaa ccattgcaac caaaaagagc cgtgatttag atattgacgt gcgtgtggtg
      901 gtagatcgta aaaccggtga tttccaaact ttccgtcgtt gggctgtggt tgaaaatgtg
      961 aataatatga cccgtgaaat cagcttagaa gcggcacaat ttgaagatcc aaacgtacaa
     1021 ttaggcgatt ttattgaaga tgaagtagag tctatcgagt tcggtcgtgt ggcgattcag
     1081 acttttggtc aggtagtcaa aaccaaaatt cgtgaagcgg agaagacgaa aatcattgat
     1141 gaattccgtt cggatttagg caaaattatt cttgccactg tgaaaaaagt aacccgtgat
     1201 aatattttcc ttgagttaca aggtaaatca gaagatatca aaggcgaagc ggtcattgtg
     1261 cgtgaagata tgatcccacg tgaaaacttc cgcccgggtg accgtgtgcg tggtgtgtta
     1321 tacgaaatca agcctgaatc aaaaggacct cagcttttcg ttacccgtgc taaaccggtg
     1381 atgttagaag agctattcaa attggaagtg cctgaaattg gcgaaggcgt aattgagatc
     1441 aaaggttcag cccgtgatgt cggctctcgt gcaaaaattg ccgtgaaaac ccacgataaa
     1501 cgtattgacc cggtaggggc ttgtgtgggt atgcgtggtg ctcgtgttca agctatcagc
     1561 aatgaattag gcggcgagcg tgtggatatc gtactttggg acgaaaatcc ggctcaattt
     1621 gtgattaatg caatggctcc ggcagatgta agctctattg tgattaacga tgaaactcac
     1681 tcaatggata ttgcggttga agcgaaaaat ctggctcaag ctatcggtcg taacggtcaa
     1741 aacgtgcgtt tggcgacaca attaactggt tgggcattaa atgtaatgac cgttgaagag
     1801 ctaacgaaga agagcgaaga agaagatggt aaagtcatca atttattaac ttctgcttta
     1861 gaaattgatg aagaatttgc tcaagtttta attgaagagg gctttacgtc tcttgaagcg
     1921 attgcttatg tgccggttga tactttaacg gcgattgacg gtttagaaga tgaagattta
     1981 gtcgaagaat tacaatctcg tgccaaaaat gcgatcacga aaaaagcgtt agaagaagag
     2041 aagttattaa aatcagctca aattgaagaa aaattattaa cgcttgaagg tatggatcgc
     2101 catatcgcat tcaaattagc tgaaaaacag attacgactc ttgaagaact tgcagagcaa
     2161 ggtgttgatg atttagccga tattgaagaa ctttcagaag agaaagctgg cgagctaatt
     2221 atggcagcac gccaaatttg ttggttcaac taagcaaaga ggataataaa atgacagaag
     2281 agaaaaaaac actttcgctt ggtggcgctc gcaaagcgag taaagtgaat acaaccacaa
     2341 ccagtggtaa agtaaaagcg gttgaagtaa aagagaaaaa accgaaaatt gatgcaaaag
     2401 cattgcaaga aaaagcagca aaagaagctg ccgcagcgaa agctagagct gaaaaagaag
     2461 cagctgaaaa gttagccaaa gaaaaagccg aagctgaagc taaagagaaa gctgcaaaaa
     2521 aagcagcaga atcgaccgct agtaaaccga tttctgtgcc ggtaatgcca agcagcaaag
     2581 caaaagaaga aaaaccgaaa gcacaacagg tacagccaaa acaagagaaa gcggttgatc
     2641 ctgaaaaaga agctaaacgc aaagaagaag ctgagttacg ccgtaagcaa gaggaattag
     2701 ctcagcaaaa agcggaaatg gaggcaaaac gtgctgcaga aaatgcccgc cgtttagcag
     2761 aaattgctcg tgaagaagcg gaagataacg gcaacgaaga ttttgatgat gagcgtttta
     2821 cttcttctta tgctagagag gctgagcgtg ataatgaccg ccgtagtgag ggaaatcgtg
     2881 gtcgtaacgg caaaggcaat gtggctaaag cgaaaaaagg cggacgtgaa gaggataaaa
     2941 acgaacgcag tgcagatcgt cgcaaccata aagaagtaaa aggcaaaggt aaacaaggta
     3001 agaaaggtgc agctttacaa caagccttta ccaaaccagc tcaagttgtt aaagctgatg
     3061 tggtgattgg tgaaactatc actgtggctg aattggctaa taaaatggca gtgaaagcga
     3121 ctgaaatcat caaaatgatg atgaaaatgg gtgaaatggt gacgatcaac caagttatcg
     3181 accaagaaac cgcacaatta gtggcggaag aacttgggca taaagtgatt ttgcgtaaag
     3241 aaaatgagtt agaagaagcg gttttagaag atcgtgatac tcacgcagaa aaagtaaccc
     3301 gtgcaccggt agtgactatt atgggccacg ttgaccacgg taaaacctcg ttacttgact
     3361 acattcgtaa agccaaagtg gcggcaggtg aagcaggtgg tattacccag cacattggtg
     3421 cataccacgt tgaaaccgat gacggcaaga tgatcacttt cttagatacc ccggggcatg
     3481 cagcctttac ctcaatgcgt gcgcgtggtg cgaaagcaac tgacatcgtt gttctcgtcg
     3541 ttgcggctga tgacggtgta atgccacaaa ctattgaagc tatccagcac gccaaagcag
     3601 cgggtgcgcc attagtggta gcggtgaata aaattgataa accggaagcg aaccctgacc
     3661 gtgtagaaca agagttacta caacacgagg ttatttctga gaaattcggt ggcgatgtgc
     3721 aattcgtgcc ggtatctgcg aaaaaaggta tggggatcga tgatttacta gaagccatta
     3781 ttctgcaatc cgaagtgctt gaattaaccg cagtaaaaga cggtatggca agcggcgtgg
     3841 taatcgaatc ttacttagat aaaggtcgtg gccctgtggc aactatcttg gttcaatccg
     3901 gtacgctaaa caaaggcgac attgttctat gtggttttga atacggacgt gtgcgtgcaa
     3961 tgcgtgatga aaacggtaaa gatgttgaat cagcaggtcc gtctattccg gtagaagtat
     4021 taggcttatc aggcgtgcct gctgccggtg atgaggcaac cgtcgtgcgt gatgagaaga
     4081 aagcccgaga agtggctctt tatcgccaag gtaaattccg tgaagtaaaa ctggctcgtc
     4141 agcaaaaagc taaactggaa aatatgttca gtaatatggc agccggtgat gtggcagaat
     4201 tgaacatcat tgttaaagca gacgtacaag gctcggtaga agcgatcagc caatccttaa
     4261 tggaactttc aaccgatgag gtgaaagtga aaattgttgg ttcaggtgta ggtggtatta
     4321 ccgaaactga tgcaacctta gccgcagctt ctaatgctat tatggtcggc tttaacgtgc
     4381 gtgccgatgc ctctgcccgc cgtgtgattg aaaacgaaaa tatcgattta cgttactact
     4441 ccatcattta tgagctatta aatgacgtga aagcagcaat gagcggtatg ttacagcctg
     4501 aattcaaaca agaaattatc ggcttggcag aagtgcgcga tgtgttccgc cacccgaaat
     4561 ttggggcgat tgccggttgt atggtgacag aaggtatcgt taaacgtaac aacccaatcc
     4621 gtgttctgcg tgataacgta gtaatttttg aaggcgagtt agaatctctc cgccgtttca
     4681 aagatgacgt atctgaggtg cgtaacggta tggaatgtgg tatcggcgtt aaaaactaca
     4741 acgacgtaaa agttggcgac caaatcgaag tgtttgaagt ggttgaagta aaacgtacta
     4801 tttagcctaa ttttgtgtag gggcgtattg catacgccct ttgtattttt tagggcgtga
     4861 agcatcacgc ccctataaaa gagaaaaata tgtccagaga atttaaacgt agtgaccgtg
     4921 tggcacagga gctacaaaaa gaagtggcgg tgattttaca acgtgaggta aaagatcctc
     4981 gcattggtat ggtgactgtt tctgatgtgg aagtatcaag agatttggcg tatgccaaaa
     5041 tttttgtgac tttcttattt gataacgatg ctgaagctat taagcaaggt atgcaaggct
     5101 tggagaaagc aagcccctat attcgaaccc ttttaggtaa agcaatgcgt ttgcgtattg
     5161 tgcctgaact gcgttttatc tacgatgaat cgctagtcga aggtatgcgt atgtctaacc
     5221 ttgtgtctag tgtaatcaaa aatgacgaag aaaaacataa agaggactaa ttgtgtctag
     5281 acctcgcaaa cgtggccgtg atattcacgg cgttttctta ttagataaac cacaaggaat
     5341 gtcttcaaac gatattctgc aaaaggtaaa gcgaattttc caagcaaata aagccggaca
     5401 taccggggcg ttagaccctc tcgcaaccgg aatgctgccg atctgtttag gtgaagcgac
     5461 caaattttcg caatttttgt tggattcgga taaacgttat ctcgtcaccg caaaattagg
     5521 cgagcgaacc gatacttcag atgcggaagg gcaagtggtg gaaaccaaag cagttgcagt
     5581 gacaaaatct caaattttgg ctgctttgga gaaatttaga ggtgatattt tgcaagtgcc
     5641 gacaatgttt tcggccttaa aacacaacgg caaacccttg tatgaatatg cccgagcagg
     5701 gattacggtg gagcgtgagg ctcgcccgat tacgattttt gaactaaatt tcattgaata
     5761 caaggctcca tttttaaccc ttgaggtgca ttgttctaaa ggtacttata tccgaacttt
     5821 ggtcgatgat ttaggcgaag agttgggctg tggtgcacac gttacgatgc tacgccgtac
     5881 agcagtggca gattatccga ttgaaaaaat gatgagctat gcagatttgc aaaatttagc
     5941 cgaaaatcaa ccgcttgcag agttagacca gcacttattg ccgatggata cggcagtttc
     6001 tgcattagca aaaataaatt taagttcaga gcaaactaaa gcggtgggtt ttggtcagag
     6061 aattagattt gataatctga accaacttta tggtttggta cgtttatatt cagaagatga
     6121 acaatttctc ggtgtggcag aaatcaccaa tgatactgtg attcgtccta accgaatgat
     6181 taacttaacg gaataagaat gaaatcgata atttttacct tacctttagc gattatcact
     6241 ttagccttag ccggttgttc aagtgataat caagaaagtt atgccggctt aaacttagat
     6301 gctaaataca gtaaatctcg ctcggtcagt cattttaatg attatgtgca ttttctgaaa
     6361 cagaaggcag caggggcagg agtgtctgca aaaaccttaa atgctcagaa attcattcat
     6421 tacaacgcca gagcggtgca gttagatcaa cagcaagctg cacgaagacg tgacccgaat
     6481 gtaccgcctc cgccaccaaa tccaagtttt aaacaaagta ttaactcaag cgaaggtgga
     6541 tcaggcaagt gaacgttggt atgaatataa tagccagctt acccatgcca gccaaaaata
     6601 tggggtacaa aaagagtata ttttggcact ttggggaatg gagagcagct ttgggcgtaa
     6661 ccaaggggat tttgatgtac tttcggtgtt ggcaaccctc gcctttgatg gtcgccgtga
     6721 gaagttattt acgcaagaat ttgtgaatgc gatgaaaatg ctcgatgacg gcacaattaa
     6781 acgctatgaa ttgcttggat cttgggcagg agcgatggga caaactcaat ttatgccaac
     6841 ttcttactta acctatgcag ctgatggcaa taatgacggt aaaaaagata tttgggagaa
     6901 tgaagccgat gcatttgcct cgattgccaa ttatctttca acagtaggtt gggataatca
     6961 actaccttgg ggtgttgagg ttaaattagc ttcaccgatg gatttaagtt tcgccggtct
     7021 tgaaaccaac aaagcgaaaa cattggcgga atggcaagcc ttaggtattt atttagctta
     7081 tccgaatgga caagaatcgg caaaattagc tcaattacac ggcaaagagg tttggttgat
     7141 tcgtccggat aaagaagcgg gaagagcgtt tttagtttct aataatttcc gtactttaat
     7201 ggattggaat aaatcgaata accttggtat cagtatcgga aaatttgccg atcgaatttt
     7261 agcttcagtt ggtcaataat cgaataaaag catagtagca aagcctatct atgcttttat
     7321 tttatcaaag cgtcgccttt cttctgcaaa cgacgttgct taaaaaaatc actgatcttt
     7381 tggctacatt ctctgcccaa tacattgcca tgcacttcta agaaatggtt cattttataa
     7441 tcttcaaaca gatgatagcg tgaacctacc gcacctgttt tataatcact tgccccaaaa
     7501 accaatcgct taatccgact atgtaaaatt gcaccggcac acattgggca aggctcgagc
     7561 gtaacataaa gggtacaatc taacagacgg taattcccta aagtttgtcc tgcttgacga
     7621 attgcctgca tttcagcgtg tgcagatgga tcacagagtt gaatggtttg gttccagcct
     7681 ctgccgataa tctgttggtt tttatcaacc agtactgccc caaccgggat ttcgcctttt
     7741 gcttcggcta aatcagcgag atcaagggcg tattgcataa aggcgatgtc ttgctcggag
     7801 atctgaagtg gacaagaagt ttgatttggt ttgataaaca tagttgcaaa aaattgagtt
     7861 tttttgaccg cttgtaaaat gccccacgag ggtggggcat cgcttacttc gcttagtgtc
     7921 gtttagtcac gacgaccacg tttttcactg aaacgttttt ctttaaagcc acctttgcgg
     7981 tctttattga aacccccgtc tcggcgacca ccacgatcat tacgatcgtt accacggcga
     8041 ccacgacctt caaatggatt tgcattactt gcactgtttg cagagccaag taatgtcatc
     8101 tgcattggtt tgcttaatac acgtgctttg tgagcgaaat gttgtacgag atgatttggc
     8161 attccttgcg gcaactcaat ggttgagtat ttatcataaa gtttgatatg cccgatgtaa
     8221 cggctgctga tatccccctc gttggcaatc gctccaacaa tatgacgaac ttccacaccg
     8281 tcttcacgcc ccaattcaat gcggtataaa tccattgcca caccattgtt ttcacggtgt
     8341 tcacgaccgt tacgacgatc gtttcgctca ttgcggtcac gtccacgttc agaacgggca
     8401 gcacgcacaa ccggatccgg tggcagaatc agtttttgtt tttcttgcag taacatcatc
     8461 atcgccgctg ctaattcttc gtggtcttga tccgcagtaa ataaatcttc caacaattca
     8521 cgatattttt ctaaatcgtg atgttctaac tgcttagaaa tgcgggcttt gaatttctca
     8581 cggcgttttt ccattaaaat ttcgtggttt ggaatcgcca cttcattaat tggttttttg
     8641 attaaatttt cgatattgcg taataagcgg cgttcacgag gctcaacaaa cagtaaggca
     8701 cgacctgaac gaccggcacg accggtacgt ccgatgcggt gaacataaga ttcttcttct
     8761 aacggaatat cgtagttaat aaccaagctg attcgctcaa tatcaatacc acgggcagca
     8821 acatcggtgg cgactaaaat atctaaacga ccggagcgta atttatctaa ggtttgctca
     8881 cgggcttgct gggtcatatc gccgtttaat gccgcagtac ggtagccgtt gcgttcacac
     8941 aattcggtaa tatcaatagt accggttttg gtgcgggtaa agatgatagc agcatcaaat
     9001 tcttctactt ctaggaaacg caataacgca tcgtttttac ggaagccgtt taccagccag
     9061 tagctttgtt caatatccgg ggctgaacgc tcggttgcct taatttttac ctcttgcgga
     9121 tctttcataa aacgacgggt aatacggcga atcggctccg gcatggtggc agagaagaga
     9181 gcagtttgat gtgtttccgg taattcagcc attaccgttt caacatcgtc aataaagccc
     9241 atacgcagca tttcatcggc ttcatctaac acgatgaatt ttaattcgga taaatctaat
     9301 gtgccacggc ggatatggtc aagaatacgc ccaggtgtac ccactactac ttgtgagcct
     9361 tgttttaaag cacgtaattg aatgtcgtaa cgttgtccac cataaaccgt cactacacga
     9421 atgcctttca tattttttgc aaactgctca caagcatcgg ctacttgaat cgcaagctca
     9481 cgggtgggtg ccatcaccaa catttgcggg tggcgttgtg ttgggtcaat ttgtgccaac
     9541 agcggcagcg agaatgcggc ggttttaccg ctcccggttt gtgccatacc aagcacatca
     9601 cgaccggcta ataaatgagg aatacattct tgttgaatgg gtgatgggtt tacaaagccc
     9661 atttcattta cagcatcaag gattggttga ggtaagccta aatcggcgaa agttaaagat
     9721 tcagttgtca taaaaaacca tttgtttgga acgcacaaag cagaaaatat cgactatttt
     9781 tctgcacagc cattaatgaa aattatattg ttgaaattta ggaagaaatg aaataacaaa
     9841 gcaagtgatg ctttaaaaat cccctgagta taagcggtta aatttacaaa attttttgcg
     9901 aaattaaccg cttgtattgt cctaatcttt ccctattctt tatcaacaac gggagtcgtt
     9961 tgagtcatac cacgaatttg tgataactca aacagggcaa aacgatactc aacgaagtta
    10021 aacacttgat ttgcaagagc aagacgaaag aggctgattg cctcatcttt atgatctaat
    10081 ttgagttgtt gttttgctaa gtagaaatac gtttctgtca gtatttctgc atattgtgag
    10141 ctattttcct tagcgtcacg ttccattata gcacgaactt gagctaaatt gagcttccct
    10201 aaaaaataat ttacgatatt tgttccccag taatccgaag aaagattatt agctcgcttt
    10261 tgtaaattat ttttagcttc tgttggtgta aattccagct cattaaaata gagccataat
    10321 gctcgataag gatctttctt atcttcttca taaaaatgga gaagatcacg ctgggcttca
    10381 gaatatctgc cgctataata gaaagccaaa cctcgattga gataggtgta gctatattcc
    10441 cgatctaatt ccaatacggc attaaacgca tcaatcgaac tgtcgtaatc gccttccagc
    10501 agcagataca gccccatata gttataagct gccgccatac gttgattgag cgaaatggct
    10561 tgagcaaaat cataacgggc taacgaccat aaacctaaac tgtcgtaaat aacgcctctt
    10621 tcaaaataga ggtctgcttg ctcagagggc gttaatgggg cttcttccaa tacttggctg
    10681 agtcttacca ccattgcctc ttgttcaaag cgaagttgag gattaagttc tgccaatgct
    10741 aagtttttaa ttgaaaccaa atcggcatac cgactggtac agccgcttag cattaaaaca
    10801 gaaattaaat tcagtaataa gaaacggatt cgaaacaact taaaaagttg taccattaca
    10861 gcatatccct caattaagga aaattaaaaa ctgaataacg gacactcaaa gtccgttatt
    10921 cgcctttatt attgaactag aaattatttt cttgttctgt ttctaccaca ttttcagcta
    10981 cagtttcttc tgcatttgcc tcgttggtat tattgatgtc tttcatcgtc agacgaatgc
    11041 gtccttgacg gtcaatttct accactttca cttgtacttc ttgaccaacg gttaagtagt
    11101 ccgctacacg ctcaacacgt tcgttggtga tttgtgaaat gtggactaaa ccttctttgc
    11161 cacctaagat ggaaacgaat gcaccgaagt ccaccacacg ggttacttta ccgttgtaga
    11221 tttggtttac ttccacttcg gcaacgatct cttcaatacg agccattact gctttcgctg
    11281 cattgccgtc agttgccgca attttcaccg taccgtcatc atcaatgtcg atagaagtat
    11341 tggtctcttc ggttaaagca cgaattgttg caccgccttt accaatcaca tctttgattt
    11401 tcttcggatc gatcttcatt gtgtgaatgc gtggggcata gtcagaaata tctgcacgag
    11461 gtgccggaat ggcttgttcc attacaccta aaatgtgcat tcttgcacct ttagcttggt
    11521 ttaaggcaat acgcataatt tcaggggtga tcccttcgat tttgatgtcc atttgcagcg
    11581 cagtcacacc ggtacgggtt cccgctactt taaagtccat atcgcctaag tggtcttcat
    11641 cacctaagat gtcagaaagt accacgaatt tctcgtcttc tttcacgagc cccattgcga
    11701 taccggcaac cgctgctttg attggcacac ctgcgtccat caacgcaaga gacgcaccac
    11761 acacagatgc cattgaagaa gaaccgttag attcagtgat ttcagacacc acacgcacta
    11821 catacgggaa ctcttcagcg gttggcatca cggctaatac gccacgtttt gctaaacgac
    11881 cgtgaccgat ttcacggcgt tttggcgagc cgatacgacc ggtttcgccc acagagtatg
    11941 gagggaaatt gtagtggaat aagaaacggt cagatttttc gccggtcaat tcgtcaatga
    12001 tttgggcatc acgctctgtg cccaatgttg ctactgctaa tgcttgggtt tcgccacggg
    12061 tgaaaagagc agaaccgtgg gtgcgtggta acacactggt gcaaatatcc aatgcacgca
    12121 cggtatccac cgtacggccg tcaatgcgtg gttcgcctgc aataatacgg ctacgcacga
    12181 tttggctctc taatgcggtg atgatgtcga tgatagtccc ttcagaaaca gtttcatctt
    12241 ctgcggtaag ttgtgcaatc acctctgctt taatcgcatc gatttgctcg taacgaactt
    12301 gtttttctac gatacgataa gcatcgccaa ggcgtgtttc tgctaatgct tttactttgt
    12361 tgattaaatc tgtatttggc tctggtgcaa cccaatccca acgtggtttg cccgcttctt
    12421 taacaaattc tttgatgttt tcgattacaa cctgttgttg ctcgtggccg aacaccaccg
    12481 ccgctaacat ttgctcttcg gttaagatat ccgcttcaga ttccaccatc aacacggctt
    12541 tgtctgtacc tgaaaccact aaatctaagc ggctgatttt ttgctcgctg gtggttgggt
    12601 ttaatacgaa ttgatcgttg ataaaaccga cacgagccgc accgattgga ccgttaaacg
    12661 gcacgccgga taatgaaagg gcagccgatg caccgatcat cgcaaccaga tcaggactga
    12721 tttgtgggtt taccgatact acggtcgcaa tcacttgaat ttcgttaaag aaaccttctg
    12781 ggaaaagtgg acgcacagga cggtcgatca agcgagcgat taaggtttca ccttcgctag
    12841 gacgtccttc acgtttgaag aaaccgcccg gaatacgacc ggctgcgtaa gtacgctctt
    12901 gataatctac ggttaatggg aagaaatctt gcccttcttt tacgtctttt ttcgctacta
    12961 cggtaacaaa tacggttgtg tcgtccatac ttgccattac tgctgccgtt gcttggcgtg
    13021 cgatagcacc ggtttctaag gtcacggtgt gctgaccgta tttaaactgt tttacaattg
    13081 gagtcattga aattttcctt ttaaagaatt tactattatt gtcgttttat ttctctaaat
    13141 tgcgactagc aaaaaggctt ttaagtaaaa catttttgat agccgcctct caataacaag
    13201 aaataaaact tgtctagtat agcgtgattt ttataaaaaa tcgcctatta ttttgatctt
    13261 gcacctcagt tataaagaaa ttcaaaactt cagtgtaaaa tggggattag tttgtgaaaa
    13321 gccttgtgaa aatagtttga ttgcatcgct atttttttat tagaggtagt ttaaaactaa
    13381 atctgcttcc tttaccggct tcactttcaa tttccagtgt ggaatggtgc tgttcaagag
    13441 cgtgtttgac aatagccaga cctaaaccgc taccgccggt tttgttgcta cgagattcgt
    13501 ccacacgata gaaacgttct gtcagatggg taatatgatg ggcatcgata ccaataccgt
    13561 catcttgcac gctgaaaatc gcagtattcg gctcttgatt atcacgttga caacatattt
    13621 gaatggtaac tccttcaccg gcgtgccgta ttgcattgta aattaaattt gatacagcgc
    13681 tttggagttg ctcttgatct ccccataccg ttaaattagg ctcaatatca aacagtattt
    13741 gctgattttg gcttaaaatc tcggtatttt tttgtatggc ggagattaac gccgacatct
    13801 ggacctcatt gtgttcggaa tcattggaat gctcgatttt aaccaaaagt gagagttggc
    13861 gaagcaggtt agtcatacgt tgactttgct ctttcatcgc attgagcgct ttttgctgta
    13921 atgccatatt atgcggctcg ttttccagca tttccaaata accttgtaat atggtaagag
    13981 gtgttcgcag ttcgtggttc atatttgcca aaaaggtttg tcgtgagtgc ataaggcgga
    14041 caaaagaggt tacatctcgt gcgacaataa gtctggagtt actgtcatat cgattgagat
    14101 tcaattcaat atagcgttta ttatgagtca gcagtaccaa tgggcgatgg tgtcgttgtt
    14161 ggcggaaata gcttttaaat tcaggataaa aaatcacatt aaaaatgctt ttatttgatt
    14221 ttttgtccca ataaaactca aatagctctt gtgcaacatt attgcaccaa gaaatctccc
    14281 cgttttcttg gcaaataatg atcgcatcgg gtaagtattg aatatgctta tttaattttg
    14341 ataacagacg taaggttttg attttatctt ggcgtaaacg ttcgtgatag aatgcggcac
    14401 tttgagataa atgctccatt gtcgttactt ttgacctgcc gctttgctca ttaggtctga
    14461 gtaactgaag gagagaagat tcggtgtggt gatgccaaat aagtagaacc agtaaacaaa
    14521 aactaaacca gaaaaaataa gcagggaaaa acagattgaa tacgccggca acggctgctg
    14581 ccagcaaaag ttccaataaa aagtgtttaa gtgagagctt aactttcatt attagtcctg
    14641 aaaatggttg gaaaaacggt atcccgagcc acgtacggtt tgaatataac gctcaaaacc
    14701 atagggttca agactcttac gcaatcgccg aatataagag tctaccgtgc gatcctcaac
    14761 ataaatatca ttaccccaaa taaaatcaag caactgttca cgacgataca ctttttcagg
    14821 gtgagtcatt aaaaagtgaa gcagtttata ttccgtgccg cttaatttca cttcctgttc
    14881 ggcaaccaaa acccgttgtg cctgttgatc aagaatcaat ccgtctatat tgatgagatt
    14941 gcttggcgga tcatagcttc gccgccatac tgcttcaatc cgggcaagca acacctgtgg
    15001 tgaaaacggt ttggtaatgt agtcgtctgc accggcattc agaccggtaa tacagtcttc
    15061 ttcgctactg cgggctgtta gcataatgat tggaattttt tcggtttcag gctgtttttt
    15121 taggtattta attaaatgga tacccgaacg ccccggcatc atccaatcca gtaaaataag
    15181 ctcgggtttt tcgcccagtt tattgatagc actttggtag tcttccgctt caattacctg
    15241 atattgtttg ctgagataga gacttagcat ttcacgaata gcacgttcgt cttcaacgat
    15301 cagaattttg ttattcattc ttcctctccg atgcggtgtg acaagcggtc gatttatcgc
    15361 agaaatttgc aaatttttta ctaaaaccga ccgcttgcag attaccccat tttaccttta
    15421 atgtaatctt ccgtctgccg gaattttggt ttgtcgaaaa tttgtttggt atcaccgaat
    15481 tcaatcagct cgcctagata catataagcg gtatagtcag aacagcgggc tgcttgctgc
    15541 atattgtggg taactatcgc taccgtgtag tcgtgcttga gttcgttgat aagttcttcg
    15601 attttcatag tcgaaatcgg gtcaagtgcg gagcagggct catccagcag taacacctcg
    15661 ggtttcactg caatacctcg tgcaatacag agccgttgct gttgcccgcc ggagagactg
    15721 tcaccatttt ggtttaattt atgctttacc tcattccata aagccgcttt ggttaatgcc
    15781 cactctacac gatcgtttag ctcggatttc ggcaattttt caaacagacg aataccaaaa
    15841 gcaacattat cgtaaatgga catcgggaat ggggtcggtt tttggaaaac cataccgact
    15901 ttggctcgaa taatcgaaat atccgtatcg gaggtgagta gattctctcc atcaagattg
    15961 atttcccccg ttgcacgttg attcgggtag agctcaaaca ttcggtttag ggttcgcagt
    16021 aatgttgatt ttccacaacc ggaaggaccg ataaatgcgg tcactttatt tttggcaata
    16081 cgcatattga tatttttcag agcgtggaaa tcgccataat aaaaattgag gttgttaata
    16141 tgtaattttg tctctaaatc cgttggtaac atatagagtc cttattttgt tttgctgaaa
    16201 tacagacgag tgatgatatt aagagaaagc acaaacagag taataagcat tgcacctgcc
    16261 caagccaatg tattccagtc agcgaacggg ctggctgcgt attgataaat caccacaggg
    16321 aggtttgcca ttggtgcatt catatctaaa gaggcaaact ggttggataa tgcggtaaac
    16381 aatagcggtg cggtttctcc ggaaatacgg gcaacagcga gcaacacgcc ggtaataatg
    16441 ccggagcggg cggcacgata agagatcatt gtaattaccc tccatcgtgg gcagccgaga
    16501 gccgcagctg cttcacgcag attattaggc acaagattga gcatactgtc ggtagttcga
    16561 acaacaatcg gaatcactaa taccgccaat gctaatgagc ctgcccagcc tgaatagtgt
    16621 ttaacctgtg cgacataaat cgcataaata aataagccga taataataga aggagcagag
    16681 agcaacacat cattcaaaaa acgggtgaat tttgccaatt tggtataacg cccgtattcg
    16741 ctcaagtaag tgccggctaa aataccgatt ggtgttgcaa tcagtgttgc ggtactgaca
    16801 atcagtatag agccgacaat tgcattacgt aatcctcctg tttcatttgg tgcaggagtc
    16861 gggcgggtaa acagttccca agacagtgca gggatacctt tttggagtaa agtaacaata
    16921 atccagacca gccaaaataa gccaaataat acagcggaat aggcaagact taacatcaag
    16981 acatttttta tttttcggca ataaaagcgt cggttttggt aatttttcat tctcgttttc
    17041 cttcctgttt acccattcgt gcgattaata aacgtgaagc ggctaaaaca atcgaagtaa
    17101 taaggaacag aattagccct aggctcatca acgctgattt ttgtaatccg ccggcttcat
    17161 taaattcatt ggcgatagca gaagcgatgg aggttgaagg ggaaaatacc gaatcaggta
    17221 gcataaaggc attaccgatc acaaaggtaa ctgccattgt ttcgcctaat gctcgaccta
    17281 acccgagcat aattgcaccg attactccgg ttttggtgta aggtagcacg actttccaaa
    17341 taacttccca agtggttgag cctaaaccgt aagcagactc tttcagtaaa ggcggtacaa
    17401 cactgaaaac atcacgcata attgaggcaa taaagggaat aatcataatc gccaatacca
    17461 agccggcact gaatagcccg atcccgaaag gagctccgct aaaaagcata ccgataaagg
    17521 gaagctgacc aaatgtatct atcaaatacg gttgcacata ttcttgaaat aagggtacaa
    17581 atacaaacag cccccacata ccgtaaataa tagagggaat agccgctagc atttctaccg
    17641 ctgtgccgac agggcgtttt agccattgcg gtgcaagttc ggttaagaaa gtcgcaatgc
    17701 cgaacgagac cggaacggca atgactaatg caatgccggc agaaatcaat gtgccgataa
    17761 tcggcactaa tgcaccgtat cggtcattca cagggttcca ctcgttttcc cataggaatg
    17821 ataccccgaa tcggcttaat gcttcccaac tgccaagcaa taatgaaacc agcaccacca
    17881 ccaacaaggc aaaaaccagc aaagcaaaga aacgggttgt gtgatgaaat aacccctcta
    17941 cccaaggatg gtttaagcat ttgttatcaa ttttatgttt atgcatagtg acaactcttt
    18001 tgcaaaatgt tgtgaaaaaa cgaccgcttg ttgttagtta agctccatac aagcggtcgt
    18061 tttgcattag tagagcgaat ttccttgtcc atctttaact tccgttttcc attgagtttg
    18121 gattttctct actacattat ccggtagagg cacataatcc agttcggcag ccgcatcttt
    18181 accgtgctta aatgcccagt cgaagaattt aaatacccct ttagtttgct ctggtttgtc
    18241 gctatgttga tgaatcaaga taaaactggc ggcggtaatc ggccaggctt tttctcccgc
    18301 ttcgttggtt aaaataacgc ccataccggc tgtttggctc caatcggcgt tagcagcggc
    18361 agcagcaaac gtttcacggg aaggctgaac aaatttacca tctgcatttt gcatagaaac
    18421 ccaagccaga gcattttgtt tagcataggc atattctacg taaccaatag aatacttgag
    18481 ctgtttcaca taagctgcga caccttcatt tccttttccg ccttgtccga ccggccattt
    18541 caccgatttt ccttcgccga ctttctcctt ccattcggca gagactttag aaagatagtt
    18601 tgtccaaccg aaagttgtgc ctgaaccgtc tgagcggcgt acgactaaaa ttgtttttgc
    18661 cggcagggcg atatcttgat taagtgcgac aattgccgga tcgttccatt gtgtaatttt
    18721 gcctaggaat atatcggcaa gaactttacc ggacagtttc agttcgcccg atttaagttc
    18781 aggaatattg acaaccggta cagttccccc aatgatggcg ggaaattgca aaagtttgtg
    18841 ttctgctaat gtttcgcctt tcatcgggtc atcagaagca ccgaaatcaa ttgttttggc
    18901 aataatttgt tgctgaccgc caccggaacc gatagattgg taattgattt tattaccggt
    18961 ctctttttca tataaacctg cccatttggc gtagatagga taagggaaag aagcaccggc
    19021 tcctgtgatg gtttgagcat ttgctgcgac agaaagtgaa aaagaaatgc ctaaaacggt
    19081 aagtagttta aatcgtttgg caatcatatt gttctcctta aagttagcgt gagttaattt
    19141 cacgtgctta gtgtagaaaa agaatatgac aaaatgatga caatggtaaa aaaatcagaa
    19201 aaaacgaccg cttgtgtttc aggctagact aaacaggcta aatcgtagaa aatagccctc
    19261 gtttttttaa tataaatatc gataaaaaat gaccacagaa atcaattatg aaggcattga
    19321 acagatgccg attaagcgtt ttaccgaaga tgcttacctc aattattcga tgtatgtaat
    19381 tatggaccgg gcattgccgt ttattggcga tggcttgaaa ccggtgcaac gccgtattat
    19441 ttatgcgatg tcggaacttg ggctaaacgc ctcggcaaag tataaaaaat cagcccgtac
    19501 cgtgggggat gtattaggta aattccatcc gcacggtgat agtgcctgct ataaagcaat
    19561 ggtgctaatg gctcaaccgt tctcttaccg ctatccgtta gtggacggac aaggcaactg
    19621 gggggctccg gacgatccga aatccttcgc tgcaatgcgt tataccgaat cgaaactgtc
    19681 taaaattgca gaaattttat tgggcgagtt agggcaagga acggtggatt atcagccaaa
    19741 ttttgatggc tcgttggaag aaccgaaata tctgccggct cgtttaccgc atattttgtt
    19801 gaatggcacg atggggattg cggtgggaat ggcaaccgat attccaccac acaatattaa
    19861 tgaattggct gatgccagtg tgatgttgct ggataacccg aaagcaacgc ttgatgatgt
    19921 actaagcgtg gtacaagggc cggattaccc gacagaggct gaaatcatca caccaaaagc
    19981 ggaaattgcc agaatgtatg agcaggggcg tggttctatt aaaatgcgtg cggtgtggaa
    20041 aaaagaagag ggtgaaattg tgatttcagc cttgccgcac caagcctctc cttcaaaaat
    20101 tattgagcaa gtagcaaccc aaatgcggaa taaaaaacta ccgatggtgg acgatattcg
    20161 tgatgaatct gaccacgaga acccgattcg cattgtgatt gtgccacgct ctaatcgcat
    20221 tgattttgat gcgttaatgg atcacctgtt tgcgacgacc gatttggaga aaagctaccg
    20281 agtcaatatg aatatgattg gcttagacgg caagccggca gtgaagaatt tgctgactat
    20341 tctcaatgag tggctgagtt ttagacgtac cactgtcact cgccgcctga attaccgatt
    20401 agataaaatt ttaaaccgct tgcatatttt agacggtttg atgattgcat ttctcaatat
    20461 tgatgaagtg attgagatta ttcgcaatga agatgagccg aaagccgagt taatggctcg
    20521 ttttaatctg accgatgtgc aagcagaggc gatcctgaac ttacgtttac gccacttagc
    20581 caaattggaa gagcatcaat tacaggcgga aaaatccgaa ctggaaaaag aacgtgatga
    20641 attgcaactg attttaggct cggaacgccg tttgaacagc ctgatcaaga aagagattca
    20701 agccgatgcc aaagcatttg ccagcccacg ccgctcacca ttggttgaac gagcggagtc
    20761 taaagcgatt gcggaaagtg acttgactcc aaccgaagat gtgactgtga ttttgtctga
    20821 aaaaggctgg gtacgttgtg cgaaaggaca cgatattgat gttcaagccc taagccatcg
    20881 agccggagat ggttatcttg cccacgcaag gggtagaagt aatcagcctg tggtattttt
    20941 agacagtaca gggcgagctt atgctctcga tccaacttca ctgccttcgg cacgctcgca
    21001 aggcgaaccg cttacaggca aaatcaccct accggaaggt gcagtagtgc agcaagtgct
    21061 aatggcaagt ccagaaacta aagtgctaat ggcatccgat tcaggttacg gctttatttg
    21121 cacctttgca gatttagttt ctcgtaataa agccggcaaa gcggttattt ctttaaccga
    21181 aaatgcaaaa gtactgccgc cgcaattatt ggaaaatgat gaaaatttat cgcttgtagc
    21241 gatgagtaat gttggcagaa tgttggtttt cccggtgagt gaattacctc aactttccaa
    21301 aggtaaaggc aataaaatca tcaatatttc cgccgcagcg gcaaaatccg gtgatgaata
    21361 tttagcccgt ttattagtca tcaaaccaac caactcgctg gtctttgtgt ccggtaaacg
    21421 caaaatcaca ttaaaaccga gcgatgttga taactaccgt ggcgaacgag caagaaaagg
    21481 ctcacagcta gtgagagggc ttagtaccaa ttcaacggta gagattgttg agtaaagaga
    21541 agaagcggtt agggggtaaa tccaacaatt ttgccccaaa taaccgcttg caatggagtt
    21601 attgcttatt caaataagcc tttgtgaagc gaacgaacca catcgtctgc ttctgcattt
    21661 ttcactaagg tacaaacatt attgctgctt gctccatagc tgattaggcg aatgttgtaa
    21721 ttctcaagag tgtggaataa ttttttcgcc acaccggact gagcgtgtaa gttgttgccg
    21781 ataatagcga ctaaagcgag gtcgctttct acttgcacat tgcaatatgc gttaagctca
    21841 tcgagtaatt ccgctgataa catatctgca cctgaagagg ctgagcccgt tttatctagg
    21901 gtcaatgcaa cgctgacttc ggaagtggta atcacatcaa ccgaaatttt gtgtttcgct
    21961 aaaatcgcaa atacattcgc taagaaacct tgtgcgtgta gcattgaaag gctggaaagc
    22021 gtgagtaaaa tttggttgcg gcgtaatgca atcgcacgga aagtcgggcg tggttgtgga
    22081 tcacgggtta cccaagtgcc gccttgttcc ggtgctttgc ttgagccaac atatacaggg
    22141 atattgctac gcactgccgg taataaggtt gctgggtgta atactttcgc tccaaatgtt
    22201 gccatttctg ccgcttcgtt aaatgccatt gtgtcaatgc gttgagcgtt cggtacaaca
    22261 cgaggatcgg tggtataaat ccccggtacg tctgtccaaa tgagtacatc gttagcattt
    22321 aatacttctg ctaacaatgc agcagagtag tctgaaccgc cacgtcctaa agtagtggtt
    22381 ttgccttcat catcacgacc aataaagccc tgtgtaatca caattttgcc tgatgcgatt
    22441 aatggcttaa tcacattgtc tgattgttgc tgggttttct cgtcatttgg tgctgctttt
    22501 ccataatggc tgtttgttgc cacaacgtca cgcacatcta cccatactgc cgggtgtcca
    22561 cgttcaatta ataattgcgt gaagattttg gtggacatca tttccccatg gctgattaac
    22621 tcatcactta aggcagctga agtagcaagg ctggctgatt ctgctaatgt ttcaatacgt
    22681 tctaataatt cgtcgatttc cgctttaacg gcatcttgat gttgtaattt ttcaataata
    22741 ttgtattgaa tttggcggac tgctgcgatg acttcatcac ggtgttcttt ttcacagccg
    22801 ttagcaagtt ccactaagta attggttacg ccagcagagg cagaaagtac caccacacga
    22861 gtatttgcat cagcaattac aatattggca gaagaggtca ttgcatcaaa atttgcaaca
    22921 cttgtaccac caaatttagc cacagaaaga tgagacataa taaaaatttc cttaaaacaa
    22981 aaatagataa atattgtgtt aggggatatt cggaaaggaa atttgagtag attatacggt
    23041 tttattgttg aaccaattaa tcagagcact ccatttaata tggtgaaata acctattaaa
    23101 tgacagctga taggattcaa cctattcagc cgatgttaat tccagtctag tgaattcaca
    23161 cctcggcaat cactcccatc acttaattct caaacggccc tagttgccta aaatcaagtt
    23221 gcctgagtgt tgcacctctc ccgtaatggc aagcccatta ctctttttca acagtattga
    23281 aaagtgtgca tagaaatacc gttttattaa tgttttgtca atgaatattt aggtaatcca
    23341 atgctattta ggtaattatt taaaatatag cgataaaaag taaacatatt tctaatgttg
    23401 aataaaaact cctaaaaaat attttgaaat ggatcacaaa accaaattta tttgattgtt
    23461 ctttaatcgg tttttttcta tgcttaagtt actcattatg aggtattttt ggaggctttt
    23521 atgagtacat taacctattt acaaaaaatt gggcgtgcat taatggtgcc ggttgctgca
    23581 ttaccagcag cagcattatt gatgggtata ggctattgga ttgatccgga cggctgggga
    23641 acaaatagcc aattagcggc attgctgatt aaatctgggg cggcaattat tgataatatg
    23701 tctattctat ttgccgtggg tgtggcattt ggtatgtcaa aagaaaaaga tggggcagca
    23761 gcattatccg gtttagtcgg gtatttagta attaccactt tgttgtctcc ggggaccgtt
    23821 gctcagctaa aagggatcgc agttgatcaa gtgcctgcag cattcggtaa aatcaacaac
    23881 caatttatcg ggatcttagt aggggttatt tcagccgaaa tttataaccg tttctaccaa
    23941 gtagagttac cgaaagcatt gtctttcttt agcggtaaac gattggtgcc gattgtggtg
    24001 tcatttatct tgctttatgt ctggccggtt atttttggtg ctttagtctc ttttggggaa
    24061 tctatcaaag atatgggggc gttaggggca ggtatttatg ctttctttaa tcgtttatta
    24121 attccggttg gattacacca cgcattaaac tcggttttct ggtttgatgt tgcaggcatt
    24181 aatgatattc caaacttctt aggtggggca aaatcattgg ctgagggcaa agcagtgata
    24241 ggagtaacag gtatgtatca agctggtttc ttcccgatga tgatgtttgg cttacctgcg
    24301 gcagcattag ctatttacca cagtgcgaaa ccggctaaga aaatgcaagt agcctctatt
    24361 atgtttgctg ctgctttagc ttcgttcttc accggcatta cggaaccgct tgaatttgcc
    24421 tttatgtttg ttgcacctgt gctttatgtg gttcacgcat tattaaccgg attatctgtt
    24481 tatattgcag cctcaatgga atggattgcc ggtttcggtt ttagtgcagg gttggtcgat
    24541 ttagtgttat cgacacgaaa tccgctggca gtgcagtggt atatgttatt ggcccaaggt
    24601 atcgtattcg cagtaattta ctatgtattg ttccgagtag tgatccaagc ctttaattta
    24661 aaaacgccgg ggcgtgagga agatagcgaa gtacaaacag tgaatactgt ttctcgtagc
    24721 gaacaagctg ccaaatttgt tgaagcatta ggtggtaaag agaatattac cagcattgat
    24781 gcttgtatta cccgcttacg cctaacctta aatgatacgg cgaaagcaga cgaaaccaaa
    24841 ttgaaagcgt tagggtcaaa aggtaatatc aaattaggcg agaatggcta ccaagtgatt
    24901 ttgggaccgg aagcagaatt tgtggcagat gcaatgaaaa agctgaatta atctctgctt
    24961 aatcacaagc ggtctgattt gcatattttt ttgcaaatca gaccgcttgg ctttttatgc
    25021 ttttttattg gttggtgctt gtttgtgaga ggccattttt tggcggattt cacggcgttg
    25081 tttggaaagt tccgcattac ggatcatata atcatctact cgattttcat agtcatcacg
    25141 catattggaa ataatcgctc gaacttcctc gatagacatt tcgtcggtaa tatattgcgt
    25201 taggttatct aataaaagta cacgcttctg attatctcga attttgcgat cattatccgc
    25261 cgtttcacgg tgcagtttgt tttttagacg ataaagatgc acatattcca gcatatcgtg
    25321 gaatgattta ttcacttcag acatatcaat ctcccttgat gtagtgttga attaagtaag
    25381 ctcgtaaatc ctattctttt tccgtcaatt cgtcaaaaaa atttatcaca ttttatgatt
    25441 taaattaatt ctcgcgaata acgctaaatt tgtagtgata tctagggctc aaaaatggta
    25501 atatcttact tatttcagtt attggtacag ggtttcatta tgtcatcaaa tattgctcct
    25561 agagagtttg cagctattga tttaggctcg aacagctttc atatgattgt agcccgtatc
    25621 gtaaatggtt ccattcaagt gctttcccgt ttgaaacggc gtgtacggct ggcagatggg
    25681 cttgatgaaa acttagtatt aagccaagat gcgattgata gaggcattgc ttgtttagct
    25741 ttatttgcaa aacgtttaga agggttctct gcagagaatg tgaaggtggt tggcacttat
    25801 accttacgta gagcaatcaa taaccaagct tttctggagc aagcgaaagc agtatttcct
    25861 tacccgattt ccattatttc cggacaacaa gaggcgaggc tgatttattc gggcgttagc
    25921 catacccaac ccgaaactgg cagaaaatta gtggtagata ttggcggtgg ttcaacggaa
    25981 atgactattg gtgatgactt taccccgatt cgagcggaaa gtcgtcatat gggttgtgtg
    26041 agttttgcaa aacgcttttt cccgcagggg gaattaacga ttgagcgttt tgatagtgcc
    26101 tatcagcttg caatggaaaa aatcgaagat ttaatgtggg agtacaaaga gttaggttgg
    26161 gactatgctt taggttcttc cggtacgatt aaaaccgtcc atcaagtgct gattgccaat
    26221 ggctatcgtg atggcttaat tacagaaagc cgcctacaga aaattattga tatttgccta
    26281 gagtttaaat cgttaaatga gattaagcta cgaggcttac ttgatgaacg agcagatgtg
    26341 ctagttccgg gcttagctat tcttttagca ctttttcaca cttttagaat tgatgtatta
    26401 cgttattcca acggggcttt aagagaaggg gtaatgtatg gtttggagaa aagtttccaa
    26461 gttggggata ttcgccaacg gactgcagaa gcgttagctg agcaatttgc tttagataaa
    26521 cagcaagcaa aaagggtgga gcatacggcg ttgattttat ttgaccaaat caaaacttgg
    26581 aagaaccgcc gccaagtgca agatctgcgt tcaatgctga aatgggcaag tttattacac
    26641 gagattggta ttgttgtaaa ccacaataat gtgcatcgtc attctgctta tttgattgca
    26701 aaccgagatt taccgggatt tgattttgaa cagcaaaccc ttctatcggt attaatgcga
    26761 taccacctta aaggattcaa acgctcggat attcgtagta tcaaccgtta ccaatgtaga
    26821 gatgtattaa cgttggtacg ggttttccgg ctagcggtgc tattaaaccg ttctcgccaa
    26881 gcgactgtag tgccgaaaca gttgaatttg gtgattgaaa ataatgcttg gcaacttacc
    26941 tttgaacagg gcttcttagc gaataatcca ctgatttcaa tagatttaga agaagagcaa
    27001 aaaatattgg cgagtacaaa tcttcagttt gcggtctctt aacatttaaa tgaaggaagc
    27061 gaaggcttcc tttttcgttt attgcattcc cttttgcgaa caagcggtca aatttagcta
    27121 atttt
//
LOCUS       DANLUA010000009        26909 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLUA010000009 DANLUA010000000
VERSION     DANLUA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26909
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            5..1339
                     /locus_tag="PXB44_000346"
     CDS             5..1339
                     /locus_tag="PXB44_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5280863.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(1419..1829)
                     /locus_tag="PXB44_000347"
     CDS             complement(1419..1829)
                     /locus_tag="PXB44_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5280864.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(1952..2629)
                     /locus_tag="PXB44_000348"
     CDS             complement(1952..2629)
                     /locus_tag="PXB44_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5280865.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            2747..3559
                     /locus_tag="PXB44_000349"
     CDS             2747..3559
                     /locus_tag="PXB44_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5280866.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(3607..3876)
                     /locus_tag="PXB44_000350"
     CDS             complement(3607..3876)
                     /locus_tag="PXB44_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5280867.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(3849..4526)
                     /locus_tag="PXB44_000351"
     CDS             complement(3849..4526)
                     /locus_tag="PXB44_000351"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5280868.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(4628..4885)
                     /gene="rpmA"
                     /locus_tag="PXB44_000352"
     CDS             complement(4628..4885)
                     /gene="rpmA"
                     /locus_tag="PXB44_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL5280869.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(4906..5217)
                     /gene="rplU"
                     /locus_tag="PXB44_000353"
     CDS             complement(4906..5217)
                     /gene="rplU"
                     /locus_tag="PXB44_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL5280870.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            5495..6493
                     /gene="ispB"
                     /locus_tag="PXB44_000354"
     CDS             5495..6493
                     /gene="ispB"
                     /locus_tag="PXB44_000354"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL5280871.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            6596..7342
                     /locus_tag="PXB44_000355"
     CDS             6596..7342
                     /locus_tag="PXB44_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL5280872.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            7759..8694
                     /gene="fabD"
                     /locus_tag="PXB44_000356"
     CDS             7759..8694
                     /gene="fabD"
                     /locus_tag="PXB44_000356"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL5280873.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            8711..9175
                     /locus_tag="PXB44_000357"
     CDS             8711..9175
                     /locus_tag="PXB44_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280874.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            9196..9918
                     /gene="fabG"
                     /locus_tag="PXB44_000358"
     CDS             9196..9918
                     /gene="fabG"
                     /locus_tag="PXB44_000358"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5280875.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            10037..11335
                     /gene="brnQ"
                     /locus_tag="PXB44_000359"
     CDS             10037..11335
                     /gene="brnQ"
                     /locus_tag="PXB44_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL5280876.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            11381..12205
                     /gene="mutM"
                     /locus_tag="PXB44_000360"
     CDS             11381..12205
                     /gene="mutM"
                     /locus_tag="PXB44_000360"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL5280877.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(12288..12722)
                     /gene="dtd"
                     /locus_tag="PXB44_000361"
     CDS             complement(12288..12722)
                     /gene="dtd"
                     /locus_tag="PXB44_000361"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL5280878.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(12697..13542)
                     /locus_tag="PXB44_000362"
     CDS             complement(12697..13542)
                     /locus_tag="PXB44_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL5280879.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(13544..14782)
                     /gene="proA"
                     /locus_tag="PXB44_000363"
     CDS             complement(13544..14782)
                     /gene="proA"
                     /locus_tag="PXB44_000363"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL5280880.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            15066..16949
                     /gene="mutL"
                     /locus_tag="PXB44_000364"
     CDS             15066..16949
                     /gene="mutL"
                     /locus_tag="PXB44_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL5280881.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(16968..17237)
                     /locus_tag="PXB44_000365"
     CDS             complement(16968..17237)
                     /locus_tag="PXB44_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5280882.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(17224..17490)
                     /locus_tag="PXB44_000366"
     CDS             complement(17224..17490)
                     /locus_tag="PXB44_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5280883.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            17930..18883
                     /gene="miaA"
                     /locus_tag="PXB44_000367"
     CDS             17930..18883
                     /gene="miaA"
                     /locus_tag="PXB44_000367"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL5280884.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            18952..19224
                     /gene="hfq"
                     /locus_tag="PXB44_000368"
     CDS             18952..19224
                     /gene="hfq"
                     /locus_tag="PXB44_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL5280885.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            19244..20629
                     /gene="hflX"
                     /locus_tag="PXB44_000369"
     CDS             19244..20629
                     /gene="hflX"
                     /locus_tag="PXB44_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL5280886.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(20851..>26909)
                     /locus_tag="PXB44_000370"
     CDS             complement(20851..>26909)
                     /locus_tag="PXB44_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5280887.1"
                     /translation="KVIFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAASILGG
                     ATLISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDSITNPY
                     NYFNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNNRKSRG
                     KPKASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMRQSAAI
                     ADYSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRRSIYDG
                     QNNTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINPDTAKV
                     ANNAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNASKAWKS
                     NAISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANVSKANA
                     TAVGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSGIRAAA
                     LGYNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSSFAGDE
                     NVVDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFNITADH
                     SGLGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKDGSVGV
                     KGADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDGESKTR
                     IVYEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFDAGKNI
                     MTQVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSNVTLTE
                     DGLSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVTEVTTD
                     AFGLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGKDGEPG
                     KVGIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDGSIGLN
                     GTNGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVATLNDG
                     LHFKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLKDLNSA
                     TFKPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIVNVKSG
                     IDGLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKGFSIAA
                     DNGETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGVKGADG
                     TVGVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTRIVYEK
                     PDGTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVVNNNGA
                     LTVKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQDGEIS
                     ATSKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIITKEGNN
                     VTFGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGSIGATG
                     KDGASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPGENGKT
                     RIVYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDKTASEN
                     FKSASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPSVTKGG
                     IDAKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKGAQRVN
                     NNGKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNELNIAG
                     SKNAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALAAGNLY
                     HVTQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAAASVGY
                     QW"
BASE COUNT         7838 a         6062 c         5293 g         7716 t
ORIGIN      
        1 tcttatgctt tatgcagaat ttttactttt attagccttt ttatatgcag gtagccgtta
       61 cggtggcatc ggtttaggtg tggtttccgg catcggctta tttgttgaag tgttcttctt
      121 aggaatgccg ctttccgctc cgccaattag cgtgatgctc gtgattttgg ctgtagttac
      181 ttgtgcctcc attctagaag ccgctggcgg tttgaaattt atgttgcaag ttgccgaacg
      241 cattttgcgt agcaacccaa aacgtattac ctttcttggc ccacttgtca cttatctgat
      301 gaccttaatg ctcggcacag ggcattcggt ttactctatt atgccgatta tcggtgatat
      361 tgccttaaaa aataaaattc gcccggaacg cccgatggca gcagcctctg ttgcttcaca
      421 attaggtatt accggtagcc cactttctgc tgcagttgcc tattacttaa ccgaaatcgt
      481 taaattaccg ggctttgaag gggtgacttt attgaatgta gtcggcgtaa ccatcaccgc
      541 aaccttctgt ggtgtgattg cgatgtcatt atatagctta cgtcgtggta aagagttgga
      601 agacgatcct gagtatcaac gccgtatgca agaccctgag ctacgcaaac aaatcgagca
      661 aacgagtgca acgtctttag acgaaaaact accggcaagt gcgaaaaact cggtttatct
      721 gttcctagcc gctattgcaa ccattgtgat tattgcaatg attccggcta ttaaaccaac
      781 ggttgctgct acaggaaaag ttgccggtat ggatcaaatc attcaaatcg tgatgctaac
      841 tttcgggggt ttaatcttaa ttttaactaa aaccgacccg aaaaaagtac caaacggcat
      901 cgttttcaaa tccggtatgg tagcaaccat tgcgattttc gggattgctt ggatgagtga
      961 tacttacttc acccatgcaa tgccagcgtt caaagaaggg gtaacagaaa tggttcgagc
     1021 ccaaccttgg acattcgcct tcgccttgtt tgcggtatct gtagtagtaa acagccaagc
     1081 cgtaaccgca aaaatgttat taccggtagg cattgcaatg ggcttgcctg ctccattatt
     1141 agttggttta atgccggcaa cttatgctta cttctttatt ccgaattatc cgtctgatat
     1201 cgcaaccgtt aactttgacg taaccggtac aactaaaatc ggtaaatatt acttcaacca
     1261 cagctttatg gctccgggtt tagttggcgt aattactgcc tgtattgtcg gtatcgccgt
     1321 tgcaaatgtg ctaatttaat tttaacaaaa aaacaagcgg tcagattaag taaatttttt
     1381 accaaatctg accgcttgat tatttactgc aaaaccaatt aggctttgtt aaagagtttt
     1441 gcatctaacg agtattttcc gccaccgata aaaatatagg ttaagaataa taccgaatac
     1501 acgcctgcta attcaccgcc gtttaataaa ggtaatagca cattgccttg agctccgtga
     1561 gcaataaaga acgcgtatgc catcatacct gaaaggataa acgctgtttg gcgggtaaat
     1621 agacctaaaa taaagaaaat tccgcccaca atttccaaaa taccgcctac accataaatg
     1681 gaggcaaggg gtacactgcc gtttccgttg gtcatcgaca ttggaaattc aaaaaatttc
     1741 gctgtgccgt gtaataaaaa catataaccg gcaacaacac gggtgattaa aaacgctaaa
     1801 ggttgtaaat ttgttaaagt agagttcatt tgctttcctt aaaataaatt gtgatataaa
     1861 ggtcaattga ttgaggcagg cattctattt taaaaaccta tacttcacaa tccagccgtc
     1921 atgaaataga ttatttacca ttaataaaca attaggctct cgctaacacc caaacctgaa
     1981 tttctactac tccagctttg agtaactcgg cacaaattac atttaaggtg gaacctgtgg
     2041 tgagcacatc atcaacaatc gccactcgtt tataggcttt taacggttga tagaaaaatg
     2101 ccccacgcag atttttccgc cgttcagcag cggtcaattc tctttgtgag acggttgctc
     2161 tcacacgttg taaactctgg gtatccagcg gaatctgtaa ccacttcgat aaccaatcac
     2221 acaataattc gctttgatta aaccctcttt tccaatgtct gtgccaaaaa agtggtacag
     2281 gcataatcac ttcaggcaag ctcagaacct gttccctttg agcttgtttg attgccagca
     2341 gcaattgtcg ggcaagggct tgatcccacc aatattgctg ctggaactta aattgatgta
     2401 cccaatgtga aagtggcgct cggtattcat ttaccctcac taatttatgc cacttcggtt
     2461 catttttcaa acaatagcca caagcgtttc gaaactccac cgcactcatt ccacaatgac
     2521 gacaataagg ggttggtttt agcaattttt ggcaatggct gcaaatgcct tgcacgccaa
     2581 tagcgagtgg atgattacaa tgaaagcaac ggaagccgaa taaattcaca tttcctcctt
     2641 ttatttgcag attatcaaat ttagccttat tttttccggc tatttttgtg acatcgctcg
     2701 aaaaaagagt aaaatctcat tatcagtaaa taaaaaggaa accgagatga aatccctcca
     2761 aaaaccgatc aaaattgtct ttttcgatat tgatgacacc ctctatgtaa aacataaagc
     2821 ctatattcca aaatcaatta ccgagcaggt attacctcgt ttaaaagcca aaggcattat
     2881 tccggccatt gccaccggca gaaatcacgg ctgttttcct gaagctctca aaccttattt
     2941 aaaccctgaa atcggttttg agctgtttgt taccattaac gggcaatata atttctataa
     3001 agatcaaata attagtgatt attcgttaag ccgtgagcaa attgaacgta ttatcgaaaa
     3061 agcgaatgct ttgggtattc tgtatgcctt tattacgcca atgacgattg cagtttccga
     3121 aaccaatccg attgtgcacg aagcggtttt accgatcaca ccgaattatt tgatcgatcc
     3181 acactactac gaaaaagaga aaattgtgca aatgttactg ttctacaccg aagagcagaa
     3241 acaagcggtc gaaaatagtg gcatttttgc aaatgattta aaagaggctc gctggcaccc
     3301 caatgcggta gatattctgc gaaaagaaaa ttcgaaagct cgtggcattc aagatgtatt
     3361 agcaaccttg aatattgaga ttgaaagtgc gatggcattt ggcgatggta ttaacgactt
     3421 ggaaatgctc ccgaccgtag gtgttggtgt ggcaatgggt aatggcgtgc cggaattaaa
     3481 agcggtggca gatttcatca ccaagccgct tgaggaagac ggcattctat acgctcttga
     3541 aactcttgag gtgatttaaa agcaagcggt tgtaagtttt tctccattta caaccgcttg
     3601 tagtttctaa ttacgcctaa atagcttatc gcctgcaatc gctaaaatcg ccccactgac
     3661 gaccaccaac gccccaagat aactgatcca attcatatcc ggcttggcaa aaatatgcgg
     3721 ataaaaatag tagctaaggt tggaaaagat catcgtaaaa atcggcaaca tcgtggttac
     3781 aatgctaacc tttgaagtat cccaatgatt caacgcctca ccgtatgaac cgtagccaat
     3841 taaggtgttc aacagcaata aataaagcaa ccccacagaa aaccatcctc aagattgcct
     3901 aactgtgccg gtgaggcaaa cggtaacatc agcagagaac agccgatgta aataataaac
     3961 agcacttgct gagagttaaa tttcgctaat aataattttt gagccacgcc gtatagcacc
     4021 caaatcaagc tggctccggc actcaataac actccgagag cgtaggtgtt gagctgtaaa
     4081 atttcagcaa atttatcgtt aaaaaatgcg acaagccctg caatcaataa cacaaatcca
     4141 atcttttgaa acaagccgaa cttttcctta aacaataata cgcctagtag aatcatcgaa
     4201 aatggagcta actgccacaa tacttggtta atggtgggcg aaacatagtg tagagcctct
     4261 gcaaacaaaa agaaatttgc cgacagacca cacaccccta acaaaattaa accgagttct
     4321 tttttgctac aagcggtcaa tttgggtaaa cgttttgcaa atcctagaat cagcaatagc
     4381 cctactgatg ccacaacgaa cctaacccaa actaaagttt gggcatctat cactttcaat
     4441 acttgctggg cggcaatcgg tagcgttccc caggtcattg ctgcggttaa tgcaagtgaa
     4501 aagccaagta aggggcgttg tttcatcgtg gaaattcctt tttctattct atgtttctat
     4561 attttagaaa gattgagata caaaaaaacc ccgccattgg cgaggtctct tattgatttt
     4621 gaaatgatta gtcagcaatg atgcttacat atttacggct tttctcgcct ttcacttcaa
     4681 atttaacttt accgtcagca gttgcaaata aagtgtggtc tttacccata cctacatttg
     4741 aacctgcgtg gaatttagta ccacgttgac gaacgataat gctacctgct aatacagatt
     4801 caccaccgaa acgtttaaca ccaaggcgtt tagcttcaga atcacgaccg ttacgagttg
     4861 aaccaccagc ttttttagtt gccatcttct agatctcctc tgaaattatg cttgaatccc
     4921 agtgattttc acttctgtga accactgacg atgaccttgt tgtttacggc tgtgtttacg
     4981 acgacggaac ttaacgattt taactttctc gccacgacct tgtgctacaa cttcagccac
     5041 tacttttgca cctgctacaa ccggtgcacc gattttaaca tcttcaccgt taacgaccat
     5101 taacactgag tcaaattcaa ctttctcacc agttgcaagt tcaagttttt ctaaacgaac
     5161 tacttgacct tcacttactc ggtgttgttt gccgccactt tggaaaactg cgtacatatt
     5221 tactccgcta tgtgccaact gcttttgctt ttaggcacta aattaattca taaaaatagg
     5281 gttgcaattc tacggaaaaa gattcgaaag agcaagagcg attttgagaa ttttttgaaa
     5341 tttgcaaaaa atggtaaaaa tactaccgct tgtttcaact gcgactaaaa aacaatagac
     5401 tgctccgatt ttacaacaaa atcataattc ttttttgaac acaggctaaa ttaaggtaga
     5461 atctactcaa ttttcgtctt actttatgga ttcgatggct gtgcaacttt tttctttaga
     5521 acaaattcaa gcccttgcga atgaagatat gcaagcggta aatgccgaaa ttttagctca
     5581 acttaattcc gaagtgctac tgattaacca gctcggacat tatatcatcg caggcggtgg
     5641 gaaacgtatt cgcccgttaa ttgcggtgct tgcggctcgt gccttgggtt atcgtggtaa
     5701 aaagcatatc acggcggctg cttttattga gtttgtgcat actgctacac ttttgcacga
     5761 tgatgtagtc gatgaatccg atatgcgtcg tggcagacca accgcaaacg cccattttgg
     5821 caatgcggct agtgtattgg taggggattt catctatacc cgttcgttcc agatgatgac
     5881 ggatttagac tctctgcgtg tgttaaaagt gatgtctgcc gccaccaatg cgttggcaga
     5941 aggggaagtt cagcaattaa tgaacgtgaa cgacccaaac accactgaag cgaactatat
     6001 gcgggtcatt tacaataaaa ccgcccgctt gtttgaagcc gccactcaat gcgtggcgat
     6061 tgcggctgat gcttctgagg aaattgaaac cgcattaaaa aattacggtt gctacttagg
     6121 cacggcattc cagctgattg acgatatttt agactactcc gctcaaagcg aaagtttagg
     6181 caaaaacgta ggcgatgact ttgtggaagg taagccaacc ttgccattgc tccacgcaat
     6241 gcacgctgcc gacaaagcag gcaaaactac tcaagcagag ctgattcgca atgctatcga
     6301 aaacggcggc gaattggaac aaattccaca agtattggca attatgcaag aacatcaatc
     6361 tctggattat gccatgcaaa aagcccgtga agaagcccaa aaagcggtgg attcattagg
     6421 ggttttaccg gaaagcgagt acaaacaagc cttggtttca ttggcgtatt tatcggtgga
     6481 tcgctcttat taatttgcaa aaaatcatat aaatttaacc acttgtaggg gcaaatgata
     6541 tttgccccat aacctataat tcttttgggc aaatgttatt tgccccaaca taacaatgac
     6601 tgaacaaaac caacgacccc aagaacatat tgaaagcgta cgagcgagac gccgtgctca
     6661 aaatcagaaa aaaggcccga atgccccgtt tgtgcgtgag aaattaacgc tgccaaacgg
     6721 acataacaaa ttactgctgc actcttgctg tgccccttgc tcgggtgagg taatggaggc
     6781 tattcacgcc tccggcattg agtttacgat ctatttttat aacccgaaca ttcacccgct
     6841 gaaagagtat ttgatccgca aggaagagaa tattcgcttt gccgaaaagt ggggtattcc
     6901 gtttatcgat ttagacgatg actacgaaaa cgaccgcaaa gagtggttca aaaaagccaa
     6961 aggaatggaa tgggagccgg aacgaggcat tcgttgcaca atgtgctttg atatgcggtt
     7021 tgaaaaagca gcggaatacg ctcacaacaa cggtttccct gtatttacca gttgcttagg
     7081 catttcccga tggaaagata tgaaccaaat caacgactgt ggtcaccgtg cagcagccaa
     7141 atttgatgac gtggtttact gggactacaa ctggcgtaaa ggcggcggct cacaacgaat
     7201 gatcgaaatc agcaaacgtg aacgtttcta ccagcaagaa tactgcggtt gcgtctattc
     7261 gttaagagat agcaacaaat ggcggttgga aacagggcgt cagcggattg aaatcggtaa
     7321 gctctattac tcacccgatt aaatgttact tttctttgct tcgccaaaga aaagtaacca
     7381 aaagaaaggc gacccgaact tcgcttagtt acgatttcct taacggaaaa tttgtaaact
     7441 cgctacgctc aaacagtaca aattttccta gaaatcgcaa gtcgctcaga cgggacaagc
     7501 ggtacattat tcaaaatttt ttacaaaata tagtgatgta ctcttccctt ctgaaccgcc
     7561 cgaacgtgtt tgaattggta ggaaaattta ctctgagcga agcgaatgta aattttccgt
     7621 gaaaccaatt caaactaagt gaggataagc ggtgaagtag gggtgtttct ttggttactt
     7681 tctttgcaca tgcaaagaaa gtaacgaaaa ctcagatata gatttcaact taaaaattta
     7741 cagaaggaat aataacaaat gtcaaaattc gctatggttt tccccggtca aggctcacaa
     7801 gcggttggaa tgcttgccga ccttgcgcca caatatccaa tcgtggaaga aaccttcaaa
     7861 caagcaagtg atgtgcttgg ctatgactta tgggatttag tgcaaaacgg tactgccgaa
     7921 gagttagggc aaacccaccg cactcaacct gcgttgcttg cctcaagcgt ggcacttttc
     7981 cgcatttggc aacaaaaatt ccctgagcaa aaaccagccg tgatggcagg gcatagtttg
     8041 ggtgaatact cagcgttggt gtgtgccggt gtgttggatt tccaagatgc gattaaattg
     8101 gtcgagttgc gtggtcgtgc aatgcaagaa gccgtgccgg caggcagcgg tgcgatgttt
     8161 gcgattatcg gtttagataa cgaaagcatt attaaagcgt gcgagcaagc agcaagcgag
     8221 accggtgaaa ttgtctctgc ggtaaacttt aacagcccgg ggcaagttgt gattgccggc
     8281 acaaaagcag cggcagaaaa agcaggagaa ttgtgtaaag ccgcaggtgc aaaacgtgcg
     8341 ttaccattag cggtgagcgt accttcacac tgtgctttaa tgaaaccggc ggcagaaaaa
     8401 ttagccgaag ccttgcaaaa tattgagcta aatcaaccgc ttgtccctgt tatcaacaac
     8461 gtggatgtgg cagtggaaac agatgccgat accattcgta atgcacttgt tcgccaactt
     8521 tacagccctg ttcgttggac ggaaaccgtt gaaaaaatgg cacaagatgg catcactact
     8581 ttatatgaaa tcgggccaaa caaagtccta accggtttag caggtcgtat tgtaaaagaa
     8641 ttatcagcca aagcggtaaa tgatgttgcc agctttgagg cggttgaggc ttaattttcg
     8701 ggagcgtaaa atgagtaaag aaaccctatt tatgctaata agtgcattga ttgccttagt
     8761 gattttacct ttagcttata ctgttggtat cgctaaagaa acattaggcg gaaatatggg
     8821 aatgttatcc attgctgccg gttgctcatt tttagcctat tttttaagtc taaacccgaa
     8881 aattcgccac gcctttatcg gaggttcagc tattacagct gctgctattc aagcttgggt
     8941 attatatggt gtgctattaa gcgaaaatga ccccaaaagt gcggtcgttt tatttcaaat
     9001 ttatctgttt agtagtttag ggccactgtt tttttcagct atttgggcat ttactcgccc
     9061 acgttggatt taccctaaac aaggtagtgc ggtgaaatat gccttatttg gtggcttaac
     9121 gaccgtgata aactacttga ttaccctttt tattttttat attctgacgt tataacgtca
     9181 tactaaggag aatttatgag caaaatcgca ttagttacag gggcaacccg tggaatcggc
     9241 aaagcgattg ccgaagaatt ggtagcaaaa ggctacatcg tagtcggcac cgcaacctct
     9301 gaaaaagggg cggaatctat ttcagcttat ttaggcgaga acggcaaagg cttagtgcta
     9361 aatgttgccg atgcagagtc gattgagagc gtattaaagc agatcaaaga gacctttggc
     9421 gatgtggacg tgttagtcaa taacgccggc attacccgtg acggcttatt aatgcgtatg
     9481 aaagatgagg attggttcga catcattcaa actaacttaa cttcggttta tcgtttatcc
     9541 aaagcggtat tacgcccgat gatgaaaaaa ggcggacgca ttatcacgat cggttctgtg
     9601 gtcggctcaa tgggcaaccc ggggcaaacc aactactgtg ctgccaaagc aggcttagtt
     9661 ggtttttcta aatctttagc gaaagaagta gcaagccgcg gcattacggt aaacgtggtt
     9721 gctccgggct ttatcgccac tgatatgacc gatgagctaa ccgaagatca gaaaaatgcg
     9781 attttaagcc aaattcctgc cggtgaactc ggttctccac aagatattgc caaagcagtc
     9841 gctttcttag ccagcgatga tgcccgttat atcaacggtg aaaccattca cgttaatggc
     9901 ggtttatata tgagctaatc cgctacaagc ggtctttttt caaccgagct ttgtaaattc
     9961 agagttcggt tttgttattt gttattttgt gttatcacct gctttatagg tataggtgaa
    10021 aaagtgtgaa tatgttatga ctagaaatac attagtagta ggctttttat tgtttgctat
    10081 tttctttggt gccggcaatc tgattttccc accaaaatta ggttatgaaa gtagtacaga
    10141 atttttctca gccatttcag gctttgtttt aactggagta gggcttcctc tactcggtat
    10201 tatcgtcagt gcattttata atggaggcta taaatctgca ttagacaaaa tccacccttg
    10261 gttttcttta gcattcctaa cttctatcta tttagcgatc ggtccattct ttgccggtcc
    10321 gcgcacggca gcaactgcct atgaaatggc gataatgccg tttttagggg attccagcca
    10381 agtgtcaatg ttaattttta ccttaattta ctttgccttt accctatggt tagcgctaaa
    10441 tccaaataaa atggttgatc gcattggtgc aattcttacc ccgatcttgc ttataaccat
    10501 cattggatta gttattcgag ccggctttat tttagatacc gatccacaaa tcactacgcc
    10561 taatacgaat gaacaatctt acttttttaa aggtatggtt gatggctatc taactatgga
    10621 tgcacttgct tcgttagcct attcggtaat tgtattaaat gctattcgtg ggaaaggggt
    10681 aagccaagcc gcattacaac gccaaacagt ttctgccggc ttagttgcag cagttgctct
    10741 agctgtaatt tatatttcat tgggttggat tggtaataat atgccaatta gtgcaaacac
    10801 actttcgatg ttatcagaaa gaaaattgga tctaggtact tacattctta acactgtgac
    10861 tacccaagcc ttcggtgaac tcggacgtag tgtcttagga gtgattgttt cacttgcctg
    10921 tttaaccact gctgtcggtt tagccgtatc agtcagtgaa tactttaatg aaattttccc
    10981 gaaaatttca tataaagttt atgctatctt atttactgtg attagtttta ctgttgctaa
    11041 ccaaggatta aaagaagtta ttagcaaatc cattccggta ttattagttc tctacccaat
    11101 agcaatgact gtcattttaa tgttgctgat taatttagtg attaaacttc cgttgtctgc
    11161 acaacgtcta tcactcctat tagtgacaat tgtagcgata gcttcagttg ccggtgtcgg
    11221 gtttaccgaa aacttgcctc taaaatccta ctcgatggaa tggctacctt tcgctatcat
    11281 tggagctatt attggagcaa ttatccaccc gcttaccggt cgaaagcata aataaagtaa
    11341 aggcagtgag cattcactgc ctctttttta ggagagaata atgcctgaat tacccgaagt
    11401 tgaaaccagc gttcgtggtg tatcgcccta tttggttggg caaactattg aacaaatcat
    11461 cgttcgtcag ccaaagttac gttggacggt gagtcctgaa ttaagccaaa tggcaggggc
    11521 aaaaattacc gcagtagaaa gacgtgccaa atatctgatt atccgcaccg acaaaggcga
    11581 tattttggtg catctgggaa tgtccggctc gctcgggatt ttgcaagaac gtcaacaaaa
    11641 agaaattggc aaacacgacc acgtcgatct tattacccaa aacggcacaa ttttacgcta
    11701 taacgaccct cgcaaattcg gctgttggct atgggcagaa aaagcagaag atcacgaact
    11761 gttgcgtaaa ctcgggcctg aaccgctttc aaacgatttc acagcagatt acttatttgc
    11821 aaaaagccgc aaaaaaacga ccgcttgtaa aacctttatt atgaccaatg aagtggttgt
    11881 tggggtgggg aatatctatg cgtgcgaaag tttatttatg gcaggcattc accctgagct
    11941 tgcagcacaa aacctgacta aaaaacagtg cgaacgttta gtaaaaacca tcaaagaagt
    12001 gctgactaaa gcgattattc aaggcggcac aacactgaaa gattttatcc aacctgacgg
    12061 caaaccaggc tattttgctc aagtgttgca agtgtatgga agaaaagggg aaacctgtaa
    12121 tgactgcgga aagccgattg aaactaaagt aatcgggcaa cgtaatacct atttttgccc
    12181 gaagtgtcag agattgccga agtaatctaa taataaactc tctccccctg agggagagag
    12241 acagcttgtt gaaacgttaa gtgaaacaag cagagagagg gtaaaaccta aacctgcaac
    12301 caaaaggtaa caggcccgtc attttgtagg ctgacttgca tatcggctgc aaattgcccg
    12361 gtttgggtat gaatcttagt ggcggcttgt tgatgaaagt aggcatacaa acgttcggca
    12421 tcaaccgggc ttgctccttt tgagaaacta ggacgcaagc ctttattggt atccgccgca
    12481 agggtaaact gtgaaaccac taacaaactg ccacctgctt gctgtacgtt taagttcatt
    12541 ttcccctcgc tatccgaaaa cacccgataa ttcagcactt tttccagtag cttatctgct
    12601 ttcgcttcat catcgtcttt ttccacaccg agcagcacca acaaaccgct tgcaatttcg
    12661 ccaacgactt gcccttcaac ctccactttt gcgaatttaa cccgctgaat taagcctatc
    12721 attaatgctc cccttgtctg tttccatttc gtctaaaacc gccgcaaatt gcccgccaag
    12781 taataccacc tgccaactta actgaatcca taacagcata atcggtaaca ccgctaatgc
    12841 tccataaatt gcctgataag acgggaaggc agtaatatac caaataaagg ctttcttccc
    12901 taaagtaaag aaaatcgctg ccattaatgc cccgatagca gcgtgttgaa attttacctt
    12961 tctaattgga ataaatttat acactaaggt aaaaagtagc caaattagca aaaacggcat
    13021 caattctaat aaatgctgcc cgagcgataa cgctccattt ccattaaaaa tagaaagcga
    13081 cattacatag gtgctaatcg caatgctagc accggcaagc agcgggccaa acagcaaaat
    13141 gagaaagtaa agcaagaaag agaaccacat cggacgctga cgattatcgt gccaaatggt
    13201 attcaatgcc tcatcaatac tcttaatcag cataatagta accacaaata agccgataaa
    13261 acttaccagc cccatttgtt tggcattact gacaaacaac tcaatgtatt ccgacaccat
    13321 ctcgctcgca ctcggggcaa agttgtcata aatgaacgct tttaactgct cgtttgcttc
    13381 gtggaaaatc ggaaaaaagg taaaaatgga aaacagcacg acaataaacg gcactatcgc
    13441 cagcattgtg ctataagtga gataaccggc tgaaatcgca atgttatttt cattgtaacg
    13501 cttggcaaag agcttaataa atctccacag tcgatttaac atcttaacct ctcaccaaat
    13561 aatcgccttc acacacccat ttgtaggtgg ttaaggcttc taatcccatc gggcctcgtg
    13621 cgtgtaattt ctgcgtgcta actgccactt ctgcccctaa accaaactca ccaccatcgg
    13681 taaaacgggt gctggcatta atataaaccg ccgcggcatc aacctgttgt acaaattgac
    13741 gggcaagttg ataatcggag gttaaaatcc cctctgaatg ctgcgaacca taatggcgga
    13801 tatgctctac cgcttgttgc aggtcattca ccaccaccac gttaagatca agcgataacc
    13861 actcttggct caattttgct tgatcaagcg gttcaattcc ttcaattttt tgcaaatcgt
    13921 tactgtgaat agtaacgcca agctgttgca ttttggcgac caatttcggc aaaaattcca
    13981 ccgcaatcgc tttttccacc aacagcgttt ccagcgtatt gcaagtgctt gggcgttgcg
    14041 ttttggcatt agcgatgacc tctagagatt tctcctgatc cgctgttttt tccacaaaca
    14101 gatgacaaac cccaatcccc cccacaatca ccggaatggt cgaattttct ttacagaatt
    14161 gatgtaaacc tgcaccgcct cgagggatca ccatatcaat gtagcgatct agcttcaata
    14221 gttccaataa taaggctcga tccggatccg tcaccgcttg cactgccaat ttcggcaagc
    14281 cggctttttc taacgcatct tgcactacat caaccaacac agcattggta aatttggtct
    14341 ctttaccgcc acgcaaaatc actgcattgc cggtttttag gcataatgat gccacatcaa
    14401 tggtcacatt cgggcgagct tcgtaaatgg ttaaaatcac cccgagcggc acacgctggc
    14461 gttcgatttt caaaccgctg tctaacacgc caccgtccat cacctgcccc accggatccg
    14521 ctaatttggc aacatttcgc acatcgttag caatgtcttt tagccgatct tcattcaata
    14581 ataagcggtc aataatcgcc aatgaaatcc cttgtgcttg agcaaattca atatctttgg
    14641 cattagcgta taaaatttct tctgcacgtt gctcaaggct atcggcaatc gtgagtaagg
    14701 cttggttttt ctgtaggttg ccaaattgag ccagctcaaa actagcttgt ttggcttgct
    14761 gggcaagttc tatcatctgc ataaattagg tctcatttaa tcaatattct ccataatcta
    14821 acacgttctt ttatgtgaag aaatatgcaa aagccaagaa aattggataa aatgggggaa
    14881 ttttaaaaca ttacgcaatt cccctctccc tgatttttct cctgaacgga gaaaaatcta
    14941 tccctctcct acaaggggag agggtagaat ttaactctct ccccttgtgg gagagagacc
    15001 gcctacagat tcgttcagaa tctgtaggca gagagagggg taaaaacaga taaaacttaa
    15061 acaaaatgat aaacactacc cctaaacagc taatccacat tcttccgcct caactggcaa
    15121 accaaattgc tgccggcgag gtggtggaac gccctgcgtc tgtggtaaag gagctagtgg
    15181 aaaacagttt agatgccgga gccaatacta ttcaaatcga cattgaaaaa ggcggctctc
    15241 agcttatccg cattcgagat aacggttgtg gtatcagcaa gcaagattta ccgctggcac
    15301 ttgctcgcca cgctacgagc aaaatcagca gcttggaaga tttagaagcc attttaagtt
    15361 taggctttcg gggcgaagca ttggcaagta tcagctcggt ttcacgttta acactgacct
    15421 ctcgtaccgc agagcaaacc gaagcatggc aagcctatgc acaagggcgg gaaatggcgg
    15481 tggaaattca gcctgcctca cacccgattg gcacaaccat tgaagtagca aatctgtttt
    15541 ttaacacacc ggcacggcgt aaatttctac gcaccgataa aaccgaattt gcccatattg
    15601 acgaggtggt tcgccgcatt gcccttgcca agccgaatat cagtttcagc ttaagccata
    15661 atggtaaaat cgtccgccaa tatcgcaaag tgcaagacaa ttcggtagag cagcaacaaa
    15721 aacgggttgc agccatttgc ggtgatgagt ttattcaaca ttcaacttat attgacaggc
    15781 aacacgggga tctgcaccta cacggctggg ttggctcacc ggctcttgcc cgtctgcaaa
    15841 acgacctttg ctacagctat gtgaacgggc gaatgatgcg ggataaaacc atcaatcacg
    15901 ccattcgcca agcctacggc gacagcattg caagtggtta ctatccggct tttgtgatct
    15961 ttttggattt agatccgaca atggttgatg taaacgttca cccggctaaa cacgaggtgc
    16021 gctttcatca aggaagactg gtacacgact ttattttgca aggcgtactc aacagcctgc
    16081 aacaaaccca acctacgctt gaactgcgaa atgaagtgaa cgagccgtta ccgagctatg
    16141 ttcaagacac taaccgccaa gctgcggggc aaaatatttt tgtgtcgcaa accgctccac
    16201 aagcggccga atttacgcaa aaaaatgcaa gtcgatcaag ctatacgcct aaatttgaac
    16261 gaagcaacag tgtgagttcg tccgctcaaa aatggtataa ggaattggtt ggtggcgatt
    16321 tgcaccaaac aacgccaact atcaaaacgc aaagcacgct tgttgagcca aaaacagaac
    16381 cccaaactga acataaaaca gaagccgttt cgcaaaaaat tacgcaaatt gcaccgcttg
    16441 caagccacca ttcacaagcc ttagcgattg taaaaaacca agcggtgtta attaaagaag
    16501 aggaaaattt ttacctgctt ccactggcaa cattagccga gcttaaactc aagctacaac
    16561 tggaaaaagg gcaatctcaa gcgttgttga tcccactgaa tctcagttta gatcaacaac
    16621 aaaccgaacg ctggcaacaa accaaagaag aactaactaa gcttggcttt tcgatcagcg
    16681 aaaaacagtg gcaagcccaa acacggctca cagtgctttc cgtaccacag gctgtcagag
    16741 aacaaaacct gcaacagctt ttatttgctc tgttcaacca aacacaagcg gtcaatttag
    16801 ccgaattttt tgcaaaaaaa tgcgaaaaac ccaccgcttg tacgctaagt gacgtgattg
    16861 ctctactctc agaaattgag ctttatgcca atgggaaaaa agagctggag ggaataagga
    16921 aggaagtgga tttttccaag tttttatagt ctaaagggca atacccttta gccaaataaa
    16981 tgagagtggc tgccaattcg cagcagctct agcttatcat cttccacttt tcgatagata
    17041 agaaccaaat caggttggat atggcaatcc ctaaagcctt tccattcgcc ggttaaagcg
    17101 tgatcgcagt atttctcagg taatggcttg tcattaatca gaaaatacaa cgcctcaatt
    17161 aatgaagagg ataaagactc aaccgcacat tttttatagt cacgtttaaa agcattacta
    17221 tactcaatct ctcgcattga gttgctccat taaatccgca atactatttg ctgaatgtag
    17281 cttttcctcg ccattcttaa cagcccttaa tacttctgct gtctcaaaat taggctgata
    17341 atcaaactga aaacgttttt ctgttgcgat tttcgctaat gtcattcgca tcacatcaga
    17401 gacagtcaag ccgataccgg ctaacacctc acttgcttgc tctttaattt cagggctgat
    17461 tcttacgctt actaatgcac tatttgccat caaaaactcc ttttgtatat caaatgatat
    17521 acaaaataac atttaacaaa aaattgtgca ataaaaaaga aattcaaaga aaagaaatga
    17581 atagttaaaa ttagctcgtt acaagaaatc attgagttac tcaagaaatc aaggtaatat
    17641 aaagaagtct gggcggaaca cgaacccgcc cctagcatct agccgcttac aagaacagat
    17701 agattagttg aaagagcgtg aaaatttcaa ctgctgcaca cagaaaccca agtgcaacta
    17761 aaatcaatag tagttctttc attgtaagcc tccttagtgc tactaaggat gatttaggct
    17821 caggctactg cgaatagccc taacgagcct accagtagca caatcaggat tgtaacaaaa
    17881 aagctgtgaa tttcacggct ttttttgtat ctatatacag tatattaata tgaataaaaa
    17941 accccttgcc ctttttttaa tggggccgac tgcctccggg aaaaccgatt tagctattgc
    18001 tttacgccaa gaattgccgg ttgaggtaat cagtgttgat tctgccttaa tttacaaagg
    18061 aatggatatt ggcacggcaa agccaagcaa ggaagagctt gcccttgcac cgcatcgctt
    18121 gattgatatt ttagaccctg ccgagagcta ttcggcgatg aatttccacg ctgatgcgtt
    18181 gcgtgaaatg gcagatatta cctcacaggg taaaattccg ctcttggtgg gtggcacaat
    18241 gctttattac aaggcattgc ttgaagggtt atctcccctg ccctctgccg atccagccat
    18301 tcgggctgaa atcgaaagcc gagcggcaca acacggctgg gcaacattgc atcaagaatt
    18361 agtgaaaatc gacccgattg ccggtgcaag aattaaccca aatgacagcc aacggatcaa
    18421 ccgagcgtta gaagtttttt acattaccgg caaaacgatg accgaactca ctgctcagca
    18481 aggtgaggct ctaccttata atatcttaca atttgcaatc gctcctcaag atcgtgcagt
    18541 tctacaccaa cggattgaac aacgctttca caaaatgatt gagctaggct ttgaagagga
    18601 ggtcaagcgg ttatttttac gcaaagattt gcatattaat ttgccctcga ttcgctgtgt
    18661 gggctaccgc caaatgtggg aatatttgca aggcgatatt tcgctcgatg aggcgatctt
    18721 taaaggcatt tgtgccaccc gccagctggc aaaacggcag attacttggc tgcgtggctg
    18781 gcaaggcgaa ctgacttggt tagatagttt agctccgacc agttctaaag caaaaatggt
    18841 ggaaaagatc gatcattatt taaagaattg tgataatatc taaacgcctt tggcacttat
    18901 cgcattattt ggtgtcttaa aaatcacaca actataaaaa ggaaaataac aatggcaaaa
    18961 ggtcaatctt tacaagatcc atatttgaat gcacttcgtc gtgagcgtat tcctgtttca
    19021 atttatttag ttaacggcat taaattacaa ggtcagatcg agtcgttcga ccaatttgtg
    19081 attttattaa aaaacactgt aagccaaatg gtgtataaac acgctatttc aacggttgtt
    19141 cctgcccgtg cgttatcgca taacaataac aaccaagccc aacacgctca gcctgctgcc
    19201 gaagtaactg aacaagcgga ataataacat tattactcaa ccaatgttaa acgaatcatt
    19261 gccaaactca gaagaaaatt ctgcgtttgg ctttgcttta tctgaagcaa gccaaactac
    19321 agaagccgcc tcgcctaccg atccagaaaa tcaagataaa gccattttgg tgcatctcta
    19381 tctcgggcaa cataaagatg ttgaaaattt gctggaattt aaaacgctgg cagaatctgc
    19441 cggtgtcgaa gtgctggcaa cgcttaccac cagccgccaa agttcgcata tcaaatatta
    19501 tgtggggcaa ggcaaagcgg agggaattgc cgaagcggta gaacgactcg gggcaacagt
    19561 agtgctggtg aaccatcaac ttagcccggc tcagacccgt aatttgcaat cgttatgcgg
    19621 ctgccgtgtg gtggatagaa ccggcttgat tttggatatt ttcgctcaac gggcaagatc
    19681 gcacgaaggc aaactacaag tcgagctggc acaacttcgc catttatcga cccgtttggt
    19741 tcgccgagtt actaatcaag accaacaaaa aggcggagca gttggcttgc gtggtccagg
    19801 tgaaacacag ctcgaaaccg atcgccgttt gattaaagtg cgaatccaac agttactcaa
    19861 ccgcttggaa aaagtcagca agcaacgcaa tcaaaatcga aaaaccagac aaaaagccga
    19921 tattccaaca gtatccttag tgggctatac caatgccggc aaatctaccc tgtttaatgc
    19981 gattacccaa gctggtgttt atgctgccga tcaacttttt gcgacccttg acccgacttt
    20041 acgccggatt caggtgcaag atgtcggcac gacaattctt gccgatacgg taggttttat
    20101 ccgttttcta ccgcacgatt tagtttcagc ctttaaatct accctgcaag aaaccaccga
    20161 agctacttta ctgctccacg tcattgacgg ctcagacgaa cgcaaaaacg aaaatattga
    20221 tgcggtaaat caagtgttgg acgaaatcgg agcgttagat attcctactt tattggtatt
    20281 caataaaatt gataaattag aaggtgtaga accgcatatt gaacgcaatg ccgatggcac
    20341 gccagttgcc gtgtatcttt ccgcccaaga aaatagcggc attgagctgt tattccaagc
    20401 cattcaagaa aggcttcgca gtaacttagt gtgtgaaaac ttgctcctcc cggttactgc
    20461 cggtgcaatt tacgcacaat ttaaagcaga aaactgcatt aaaaccgagc attttaatga
    20521 gttcggtgaa cggattatct ctattgaaat taacgaagtg caatggaaca aatgggtgaa
    20581 acaatttccg gatctagccg aatatattga gctggcaaaa tgggggtaat taatctcact
    20641 tctgagtaag ttatatttaa attatgcaag cggtcaattc tttacgtttt tttgcaaaaa
    20701 agtgtaaaga attgaccgct tgttttatcc attaaatcca atttagccaa taaaaaaatc
    20761 cctcaaataa accagatttc cccgttctat ttgaaggatt ttatttattc ttccccaaaa
    20821 gtggggaaga aaagcttaat caagcagtta ctaccactga taacctacgc ttgcagcagc
    20881 acctgtatca ccacggctgt tagagttaac actgaatttg atacccactt taccattatc
    20941 tgataaacga gaataaccta cagcaatagc tccttgacca cggtaagcac ctacaccggc
    21001 tgaaaccatc gatttaccag gttgtgttac gtgatataaa ttacctgcgg ctaatgcacc
    21061 tgcaatacct gcacgtaagt ctttatctac gttactgata cgattgttca gacgattttc
    21121 tgcacctttc aactgattca cattaacagc atcagtgcct ttcacacccg gagcaacgtt
    21181 cgtaatacgt gtttgcgcat tcttgctacc tgcaatgtta agctcattta cgccatcttt
    21241 tgcaacagtt gaactgatag ttgtaccacc aactttcact gaatcaaatt caagattgct
    21301 tgaggttgcc acttcaacag ttgaaccttt acgtgaaaca acgatgtttt tgccacctga
    21361 aacggccact ttaccattgt tattaacacg ttgagcacct ttctctgaag tttcagctac
    21421 attaccatca acagtgagat accagcctgt attctgcatt gcattataca actggctacc
    21481 gctcacaaca tctttacttg ttgccgttac atcaccatct ttaacgttag tgatcttcgt
    21541 gcctttcgca tcaataccgc ctttagttac acttgggccg tctttaatgg ttaaaccatt
    21601 gttatttacg gttgtgtcgc cagttgttac agaaccatct tccgttaaat caattgcttt
    21661 cgctaattgt acctctaatg tgtctgtacc attcgctttc acattgatgt tgcctgatgc
    21721 tgacttaaag ttttcagaag ctgttttatc cacaccttca cctttcactt taacttcagt
    21781 attcagtttc tgacggttct tcacatcttc attgttacca gagaagatta aaccatcatt
    21841 taaggttgca acttgttctg tgacagtttt accgtcttta tctttggtct cataaacgat
    21901 acgagtctta ccgttctcac ccggtaatcc atctttacca tttgtaccat caacaccttc
    21961 agcaccatct ttcactgcaa tatttgcact tgcaccatct ttaccgtcag cgcctttcgg
    22021 accggttaag ccgatagagc catctttacc gtttaataca acagaagcgc catctttacc
    22081 tgtagctcca attgaaccat caacaccgtc tttaccgtca cggcctgcag taattgtgcc
    22141 gtcaccttta atggttacgc catctttacc gtctttgcca gcaataccga tttgaccgtc
    22201 tttaccatct ttaccaccaa tggttaagtt gtttttcaag ccgaaggtca cgttattacc
    22261 ttctttagta ataataatgt taccgtcttt gttgtttaga tcaactgttg ctcctggttt
    22321 aacgttgcct ttgtctttac catttgctgt taagttccaa cctgcgttag caacttcttg
    22381 cacttgatat aactggctac catttaccgc atctttgctg gttgcagaaa tttcaccgtc
    22441 ttgaacgtta atgattttct tatcgcctgc attgataccg gctttagtta cacttgggcc
    22501 atctttgata gttaaaccat cattatttac cgtagtatca ccaatagtta cagaaccatc
    22561 tttcgttaaa tcaatgtctt tcgctaattt aactgttaac gcaccattgt tgtttacaac
    22621 accgatattg ttatcggaca ggttcgttaa atcagtatca ccgcctgaga tttttaacgt
    22681 ctcattaagt ttcttaacga tttctttacc gtcatcacct acgaatctta agccatcgtt
    22741 cagtgttgca acttcctcag ttgtaccgtc tggtttctca taaacgatac gggttttgct
    22801 ttcgccatct ttaccgtcgt taccgtcaag gcctttagca ccgtctttta cagagatagt
    22861 tgcacttgca ccatctttac cgtcagcgcc tttcggaccg gttaagccga tagagccatc
    22921 tttaccgttt aaagtaacac caactgtacc atctgcacct ttaacaccga ttgaaccatc
    22981 tttacctgga ccgccaatgg taaaatcgtt atttaacgta aagtcaatca cattatcact
    23041 tactgttgtg atgatgtttt tgtctttaga ggtgtactta acagtttcac ctagtttaac
    23101 gtgatcagtt tcaccattat ccgctgcaat gctaaagcct ttctcgataa tatcgttgct
    23161 tgcatttaca acatcattaa tattcgcagc gttagtacca acttctttta attgatcgtc
    23221 tgttaagtcg ttcactgaag tgattggctt accatcttta ccgttaatgc cgtctttacc
    23281 tgctaaaccg tcaataccgc tcttcacgtt aacaatttgg ttgccgccat tgtctagacc
    23341 gtcttcagta agcttaactg ctttaccatc tgtgccatct tttggcgtga tagttacgcc
    23401 attattattt actactgttg ttggaccttt accttcttga ccattctctc catcttgtgg
    23461 tttgaatgta gctgagttca agtctttaag atctttagct aattgaacag ataatttacc
    23521 atctttgctg tttacgccga tgttatttgc agttagctta tctttatcag ctccaccaat
    23581 gatttctaag gtttcattca atttcttaga tactagtgtt gcatcatcac ctttaaagtg
    23641 taaaccatca tttaaagtag ctacttgctc tggttcacct ataggatcac cattgccatc
    23701 tgttggctga tattggatac gagttacacc atctttacct ggtaaaccat ctttaccaac
    23761 aagccctggt ttgccctcag tacctttaat ggttaaacca tctttaccat ttgtaccgtt
    23821 caagccgatt gaaccatctt taccgttaag aacaactgca gaaccgtctt taccgtttac
    23881 accgatagta ccatcaatac catctttgcc agggcgacct acagtgatag ctccatcttt
    23941 accatctaaa gcaatagcat ctttaccgtc tttaccagca ataccaactt tacctggttc
    24001 accatcttta ccaacagtaa tattattcac taggctaact tctaatgcat ggccaccatc
    24061 tttctcaaca acgtttgttt taacattgcc atcaccaatg atcttagctg tcgtacctaa
    24121 gttttgctta aactccttgt tgttctcatc ttttagacca aacgcatctg ttgtcacctc
    24181 agtaacagtc tcagtaacag tatttttcaa gtcaccgacg ttaaccgcat tgttaagaac
    24241 atcacctgct gcctcagtaa gtttaacagg ttgaccatct ttacctttta agccgctatc
    24301 aacattggtg atttggttac caccgttgct taagccatcc tctgttaagg taacattaga
    24361 tttagttgca tcattaggat ctttcttagc aatcgttaca ccatctttat tgataactgt
    24421 agaatcacct tgtgtgccga atgttgcact atctaagcct gctagatctt ttgctaacgc
    24481 cacagtaatg gtattgccaa caacctgtgt catgatattt ttaccagcat caaacttagc
    24541 ccaatcagtg tttgttgtag cacctttaat atccacctgc gtatttaatt tcgcatcgtg
    24601 aactttgcca tcattcgcac cgaattttaa gccatcgtta agtgttgcaa cttcttcttt
    24661 atcaccattt ggtttttcgt aaacaatacg agttttgctt tcgccgtctt taccgtcgtt
    24721 gccgtcaaga cctttagcac ctttctcaac tttaattgat gctttaccat cttttccatc
    24781 ttttccattt acgccatcaa cgccgtggaa tacgatggct tctggtttaa ttgttacacc
    24841 atctttacca tcagcacctt taacaccaac tgaaccatct ttacctggtt taccaccaac
    24901 agagatgtta tcggataagc caaaatcgat ggtattattt gttacggttg taacaacatt
    24961 cttatcggtt gaggtgtact taacagtttc acctaacttc acattgtctt ctttagcacc
    25021 acctcctaag ccagagtgat ccgctgtaat gttaaagcca gttttaacta aagtgtcagc
    25081 tactgcgtaa agttgtgaac cgttaatcgc atctgttgaa ttggaagcaa ttttacccgg
    25141 tgcaacaaac ttaatttggc gagcattggt tgcattaccc acactcacat aatcaccatc
    25201 aacaacattt tcatcgccag caaagctgct atattttaat gtgccaacag ttgcagtatc
    25261 ttctttagtt gcggttgctt cagtagagtc agaacctaga attacagaac cctttgtctt
    25321 aacagtaaca ttagaaccta atacgaaagt atctttacct gaaactgtat tgttataacc
    25381 taaagccgca gcacgaatac ccgaaacagt actatttaca ccggctgcaa ttgagttttt
    25441 accctttgcg gttgagttgt aagcaatagc agttgcataa ttaccttttg atttggcctc
    25501 aaaaccaatt gctgtgccac cttcatgcgt aatattggca cgtgtaccta ctgctgtcgc
    25561 attcgcctta cttacattag cactataacc tatcgcaaca gaattattac cacttgaatt
    25621 agccgtatca ccaattgcca ttgcaccatc tttgctcgcg ttagaatgcg aaccaatact
    25681 gatcactttt tctgcatgag agtttgcatt ccaaccaatg ctaatagcat tacttttcca
    25741 ggctttagaa gcattaccaa aagcaatcgc accgattcct gttgtattcg aatttacacc
    25801 aaaggcgata gaaccatcac ctgttgcact tgcatttgtt gaaaacgcca aagtatcatt
    25861 cgaatttgca cgagtattcg tacccaaagc aaatgcatta tttgctactt tcgctgtatc
    25921 aggattaatt acctgtccta attcagaaat atttgaattc tctaatttaa agccaccgga
    25981 ccaagcgtta gtaccaatgg caaatgaatg gttaccatta gttttcgctt tagtaccgat
    26041 tgagaatgca tctgttccaa atgctaaagt attattttgc ccatcataaa ttgaacgtct
    26101 attttcatga ccgtaacttc catccactgt ctgtggagta ccactaccaa tcgcaaaaga
    26161 acggtcacct agcgctgtag ccgaagcacc aatggcgaag ctagctgtag catttgccca
    26221 agatgttgaa ccaattgcag tagaataatc tgcaattgca gcagattgac gcattgcagc
    26281 aaatgagtta aatccttccg cacgtgcaga ggtacctaat gctacagaac caatacttgc
    26341 agtacgagaa aaagaaccaa ttgcaataga caatccccct ccagaatatg aataatcacc
    26401 aatggctatg cctgacgctt tcggttttcc tctacttttt ctgttattgt ctaaagctac
    26461 agcattgtgt ccaattacaa ttgctgaggt agtattagac ttatacaata atctagaatc
    26521 cttctcctta ttaacagagt tattagattc ttgaagctta gttctgtctg cataggataa
    26581 gctacctggg ttaaagtagt tatatggatt cgtaatagaa tctgtagcca aactagtgtt
    26641 tgtataaata tctataccac cattaacaac tttttttggt gctttttgtt gtacttctat
    26701 tctatcaata atcaccgccg cttctgccgg tgtagcagaa agcataaagg ctgccattcc
    26761 actgataagg gttgctcccc ctagaatgct agccgctgct aatttaatag taccgcgctt
    26821 atcagtagaa gaagcggctt taccttgcga tcgagcaagt tcagacacaa cttccatttt
    26881 ttgcgtcgtt tggttaaaaa taaccttaa
//
LOCUS       DANLUA010000010        26687 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLUA010000010 DANLUA010000000
VERSION     DANLUA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26687
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            149..361
                     /locus_tag="PXB44_000371"
     CDS             149..361
                     /locus_tag="PXB44_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL5280888.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(421..1575)
                     /locus_tag="PXB44_000372"
     CDS             complement(421..1575)
                     /locus_tag="PXB44_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL5280889.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1572..2390)
                     /locus_tag="PXB44_000373"
     CDS             complement(1572..2390)
                     /locus_tag="PXB44_000373"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL5280890.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2467..3375
                     /gene="rdgC"
                     /locus_tag="PXB44_000374"
     CDS             2467..3375
                     /gene="rdgC"
                     /locus_tag="PXB44_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL5280891.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3396..4001
                     /locus_tag="PXB44_000375"
     CDS             3396..4001
                     /locus_tag="PXB44_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL5280892.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            4017..4808
                     /locus_tag="PXB44_000376"
     CDS             4017..4808
                     /locus_tag="PXB44_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL5280893.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4812..5954
                     /gene="argE"
                     /locus_tag="PXB44_000377"
     CDS             4812..5954
                     /gene="argE"
                     /locus_tag="PXB44_000377"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL5280894.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(6101..6388)
                     /locus_tag="PXB44_000378"
     CDS             complement(6101..6388)
                     /locus_tag="PXB44_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5280895.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYEIHLEKVYIITIIHSRRLYPRPS"
     gene            complement(6381..6572)
                     /locus_tag="PXB44_000379"
     CDS             complement(6381..6572)
                     /locus_tag="PXB44_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280896.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(6862..7452)
                     /gene="yraP"
                     /locus_tag="PXB44_000380"
     CDS             complement(6862..7452)
                     /gene="yraP"
                     /locus_tag="PXB44_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL5280897.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(7462..8046)
                     /locus_tag="PXB44_000381"
     CDS             complement(7462..8046)
                     /locus_tag="PXB44_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL5280898.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(8052..8423)
                     /locus_tag="PXB44_000382"
     CDS             complement(8052..8423)
                     /locus_tag="PXB44_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL5280899.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(8398..10119)
                     /locus_tag="PXB44_000383"
     CDS             complement(8398..10119)
                     /locus_tag="PXB44_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL5280900.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            10193..11065
                     /gene="rsmI"
                     /locus_tag="PXB44_000384"
     CDS             10193..11065
                     /gene="rsmI"
                     /locus_tag="PXB44_000384"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL5280901.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(11310..12476)
                     /locus_tag="PXB44_000385"
     CDS             complement(11310..12476)
                     /locus_tag="PXB44_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5280902.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(12628..14142)
                     /gene="putP"
                     /locus_tag="PXB44_000386"
     CDS             complement(12628..14142)
                     /gene="putP"
                     /locus_tag="PXB44_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL5280903.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(14236..15024)
                     /locus_tag="PXB44_000387"
     CDS             complement(14236..15024)
                     /locus_tag="PXB44_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL5280904.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(15021..15599)
                     /locus_tag="PXB44_000388"
     CDS             complement(15021..15599)
                     /locus_tag="PXB44_000388"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL5280905.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQVSHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(15601..15792)
                     /gene="ssrS"
                     /locus_tag="PXB44_000389"
     ncRNA           complement(15601..15792)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXB44_000389"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15897..16187)
                     /locus_tag="PXB44_000390"
     CDS             complement(15897..16187)
                     /locus_tag="PXB44_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL5280906.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            16266..16823
                     /locus_tag="PXB44_000391"
     CDS             16266..16823
                     /locus_tag="PXB44_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL5280907.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            16921..17985
                     /gene="hemE"
                     /locus_tag="PXB44_000392"
     CDS             16921..17985
                     /gene="hemE"
                     /locus_tag="PXB44_000392"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL5280908.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            18132..18722
                     /locus_tag="PXB44_000393"
     CDS             18132..18722
                     /locus_tag="PXB44_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL5280909.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            18858..19130
                     /locus_tag="PXB44_000394"
     CDS             18858..19130
                     /locus_tag="PXB44_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL5280910.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFVSGKALKDLVK"
     gene            complement(19219..19779)
                     /locus_tag="PXB44_000395"
     CDS             complement(19219..19779)
                     /locus_tag="PXB44_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280911.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
     gene            complement(19925..22867)
                     /gene="rne"
                     /locus_tag="PXB44_000396"
     CDS             complement(19925..22867)
                     /gene="rne"
                     /locus_tag="PXB44_000396"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL5280912.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            complement(23324..24169)
                     /locus_tag="PXB44_000397"
     CDS             complement(23324..24169)
                     /locus_tag="PXB44_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL5280913.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            24296..25120
                     /locus_tag="PXB44_000398"
     CDS             24296..25120
                     /locus_tag="PXB44_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5280914.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            complement(25205..26668)
                     /gene="ubiD"
                     /locus_tag="PXB44_000399"
     CDS             complement(25205..26668)
                     /gene="ubiD"
                     /locus_tag="PXB44_000399"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL5280915.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
BASE COUNT         7526 a         5899 c         5527 g         7735 t
ORIGIN      
        1 agtggtgtaa tggctcaagc tgttcgtgcg ttaattaaag ttgaatcttg ttagttattt
       61 tcctcaaggc aagtgattga tagatacgtc tgtaatatcc cgtttagcta tcttttatca
      121 ctttttgtat ttatataagg aaaattttat gtcagcaatt accggcacag taaaatggtt
      181 caacgccacc aaaggttttg gttttatcac ccctgatcaa ggtggcaaag atgtattcgt
      241 acacttctct gctatcgaag caaacggcgg ctaccgcaca ttagaagacg gtgctaaagt
      301 acaatttgaa atccaagatt cagagcgtgg agcttcagcc gtaaacgtga aaacaatcta
      361 atttcagcaa attcaatgaa tttcgataaa aatgccgaca acttgtcggc atttttgcta
      421 tcaattaatc tgtacttcaa accttttcgg cacaataaaa atggcaggta gggtaaaaat
      481 tgccatcaac caaaatgtca ggcttgggga atgttgatag accattccgg caataaaggt
      541 aaatactgcc ataaaagcac agcttgccaa gccaaaatag agaccttgta atttcgtcac
      601 acgctctgcc gattgcatcg aaatatagcg aatcatcgca atatgagccg ccccgaaggt
      661 aatagaatgt agcatttgca ttacaataat cagcacgatc tctgtagtac tacccattat
      721 tgcccaacga atcactgcaa aaacggcagc taaaatcatt aaatggctga ttttccacga
      781 tttaaacaag cgtttagaga ccaaaaagaa caaaatttcc gccaccaccg ccaagcccca
      841 aaacaggcta gtcattgcgg ttgaaatact ggcatttgac caatgaatgg tgctgtaagt
      901 gtaataagcg gcgtgcgagg ctgaaattaa cgacaccgcg atcaacattt ttaaggtttc
      961 cggctctttc aataattggc gataagttac gcttgaagta ttggctttat taccttgttc
     1021 ttcaaacccg actttgggcg tagcaagctg ccctattcct aaaaaaacca agaaaccgat
     1081 gataatccac accaccgaat tttcaccaag caagccgata agatagccgg cagaaagtga
     1141 gcctaccaca aacgccattg agccgaataa ccttgctctg ccgtaatcca gcccgatctg
     1201 atgttgccaa attgaggcaa tgctatcggc tatcggcatt gcccccgaat tgaacatttg
     1261 aaacaacatt aacaccggaa aaagtagcca aatgaaatcc cctgaaaaca gcaacgccgc
     1321 cgcagccgca atattcagcc aagttaaact gcgggcggtg ggaatgagtt ggctcacggt
     1381 ttttacccgt tgtgaggcaa gcatactgcc ggcaaagcgg aacaaatagc caacggcggc
     1441 aaataagccg atcatttcag tcgaatagcc gtgatgttta agccacactg gcaaaaacgg
     1501 caacaacacg ccataggcac aaaaaaagcc aaagtaatta aatgccgccc attggaacgg
     1561 ggaaactttc atcataacga ttgctccatt tctctggctc gatgaattgc cgcccacatt
     1621 gcatcatcaa cggttttagc aagatcgtgc tgctcaaaca cctcaagggc tttcgccgtt
     1681 gtgcctccct ttgaggtcac attttcccgc aaggtcgaaa gtggcgtagt cgggttcgct
     1741 tccaccaatt tcgccgcccc caatgcgaca gattgtacaa gcgttcttgc gtcctgctcg
     1801 gaaaagccca tttgcaccgc actttgctgc attgcctcca taaaacggaa gaaatacgcc
     1861 gggcttgagc cggttatcgc aataatgtgg ttgatttgct cctcggtttc cacccagtaa
     1921 catttaccaa cggctgacat caactgttcg gcaaattggc aagcggtcgg atttaccgat
     1981 ttttttgcaa ataatccggt cattccttcg ctgattaaag acggggtgtt tggcatcgtg
     2041 cgaatgatat ttttcgcact cggcaacagc gtttccaagc gttgcacaga aatacctgct
     2101 gctactgaaa tcacccattt gttggaaaaa tcaacatcgg caaactcggc acacacttgt
     2161 gccatcatct gtggttttac cgccagcacg atcacgtcag cctgctctac cgcctcacga
     2221 tttgcaaaat ttgtcgcaat tccgaccgct tgtaactcct ctcggcgagc caaattactt
     2281 ttattgcacg caatgatttg ttcggcagaa taaccgcttt ttaataagcc ttgaataatc
     2341 gcatacgcca tatttccggt gccgataaag gcaagttttt tctcgttcat tcgcaaattc
     2401 tctactgtgt tgggttgagg tctgttaaaa taacactatt ctacacccga taaaggatat
     2461 gcaaaaatgg tttggttcaa aaatgtgatg atctataaat taaccggcga gctggcgttg
     2521 gaaagcaata gccttaacga gttacagcaa gaaacccgct ttaccccttg cagccagcac
     2581 gatatgagca aattcggctg ggctacgcca cttaccggct cggatttact gcatttctca
     2641 caagcgaacc aatttttgct gatttcacac aaagaagata aattattacc tgccaatgtg
     2701 gtgaaagccg aaaccgagca acgcattgca gaactggaag aaaaagaggc tcgtaagctg
     2761 aaaaaaaccg aaaaacaagc cgtgaaagat caggtcgttt ctgcgttatt gcctcgagca
     2821 ttcagcaaac atcaattcac cgctctttgg ttggatttag ataatcagtt ggtttatgtg
     2881 gatgcagcct cgagcaaacg ggcggaagat acgctggcgt tactgcgaaa aacacttggc
     2941 tcgttgccgg ttgtgccgat ttcctttacc ctaccgccgg cagaggtaat gacgaattgg
     3001 atcgccaaag ggcatacgcc aagttggttg aatttgttgg aagaggcaga actgaaatct
     3061 ttcgataccg acagcattat tcgctgtaaa cgccaagatt tagagacgga agaaatcgcc
     3121 cggcacttgc aagcgggcaa atttatcaca aaacttgcga ttgaatggga agggcatttc
     3181 aattgcgtgt taaatgaaga cgccacgctt tcacgcctga agtttgccga tgaagtgcga
     3241 gagaaaaatg acgatattct caaagaagat gtggctcaac gctttgatgc cgatttctta
     3301 ttaatgaccg aagagctgaa acttttcacc gaaaaaatga ttgccgaatt tggcggaatt
     3361 aaatccagaa tttagaaaaa aatttaagga aagcaatgtt agatcaaacc attatcgacc
     3421 aatatgacgc cttgattttt gatatggacg gcacattaat tgacacgatg ccaagccacg
     3481 ccaaggcgtg ggaaaaggtg ggcgaagtgt taggctaccc aatcaatcca aagccaatgt
     3541 acgaactgag tggctcaacc acttttgtga ttgcccgaga aataatgcaa cgctccaata
     3601 tccccgagca ttattttgag caagtggtac aactaaaacg ggaatttggg attgaaatgg
     3661 tgttagccaa tgccactctg ttgcctgcct ttgagattat taaagctaac gttggcaaaa
     3721 aaccaatggc aatcggcaca ggctcacacc gtgcaatggt ggaattatta gaccaaaaat
     3781 ttaacttacg tcaatatgtt tcggttattg tagattctga tgatgtaagc aaccataagc
     3841 cagcccctga aaccttttta aaatgtgcgg aaaaacttgg cattgcccca caccgctgtt
     3901 tggtgtttga agatgccgat ttaggcgtgc aagccgccct caacggcaat atgaacgtgt
     3961 ttgatgtacg tacgaatacg ataactcact caacccgtta aaaatgatag gtttatatga
     4021 aaacagaatg gattaaacat ttaattcaac aattaaccac gctttccaaa gccatcgaaa
     4081 actactttac cagcggttca tttggggtga aattctaccc ttttctaatg cacccttgtt
     4141 ttcgtaaaag ctgtgccttt ttgattttta ttattgctat ggtttcaagt tttagcgggc
     4201 tattttattt ggtggcggaa cacatcacga ttttatttag cgaaagatcg ctcaccactt
     4261 acttccacca tagcaccctt gaattgatga ttccagtcgg cacaatgata gatgaaaaaa
     4321 tcagcgaatt atcaccactt tcggtggtaa tgcgttcaat gtttgtgcta caagcagtcg
     4381 tttttttctt tatttatgca atcggcatca cccacattgc tcacaacaaa ttgcgggtca
     4441 tcggcttatt gctttcgcta tgctttgcga ttggctgctc actagttgcc ggtattcagc
     4501 caagcagtcg tggcgaattc ggcatttaca atttaggggc aagcattacg tttttaatcg
     4561 gcaatttaac gctgatcatc gcaggcttcg atctctacca cccttcaatg caatttttta
     4621 aacgctttag cattggtgcg ggcgttatcg gggcaatctg tattttaatc acgattttcc
     4681 taccaaccct gtttagcccg ttaattgaac gagcaggtat ttataccatt atgctgtggg
     4741 aaattttagc cggttttgcc atcattaaac gcctgttgaa attgccctca ttcagacaac
     4801 aaggataacc aatgaaacag attcccttta ttcaacgcta tgctcaactg attgagctac
     4861 cgaccatttc cagccttgtt gccgaagaag atttatcgaa ccgccgtttg attgaattgc
     4921 tcgccacttg gctggcagat ttcggcttta aaaccgagat tttagcggta gaaggcagcc
     4981 gtaataaata caatttactt gcgacctatg gcgagggtga gggcggctta ttattggcag
     5041 ggcataccga caccgtgccg tttgatgaag gaaaatggcg cttcaatcca ttccaattaa
     5101 ccgaaaaagc aggcaaactc tacggcttag gcacggcaga tatgaaaggc tttttcgcct
     5161 ttgtggtgga ggttgtgagc caactcgacc taacccaaat caaaaagccg attcgcattc
     5221 ttgccaccgc tgatgaagaa accacaatgc tgggggcaag aacctttgct cagcacagcc
     5281 acattcgccc cgattgtgcg attattggcg agccgacctc gctcaaaccg attcgagccc
     5341 acaaagggca tattggcgaa tcggttcgca ttaccggcag aagcggacac tccagcgacc
     5401 ccgccaaagg cattaatgcg attgaattaa tgcaccaatc tatcggttat ttaatgaaaa
     5461 tgcgtgatga actgcgagag aaataccaca atccgctgtt ccaagtcacc cacccgacaa
     5521 tgaacttcgg caatattcac ggcggcgatg ccattaaccg catttgtgcc tgttgcgaat
     5581 tgcaatttga tatgcgacca ctaccgaata tggcggtgca agatttgtat gcaatggtaa
     5641 acgagcattt aaaaccgatg ctggaacaat atggcgattt aattgaaatc cgccatttac
     5701 acgatggaat tccgggctac gaatgcgaac actcggcaca aatcgtgcaa gtggttgaaa
     5761 agctactggg cgaaaaatgc gatgccgtca attactgcac cgaagcccca ttcattcaac
     5821 aactctgccc aacgctggta ctaggccccg gctccattga acaagcccac cagcccgatg
     5881 agtttttgga aatgaaatat atcgaaccga caaaagagtt attgactaaa ttgatttatc
     5941 atttttgtgg ataaatcaac agaaacaagc gctcattttc gccatacttt ttgcaaaaga
     6001 atcggcaaaa atgaccgctt gatatttagt attgagtgac ctataaactt ccatacttca
     6061 tcattgattt aaggagattc tgttagatag gtattaatct ctagctaggt cttggataca
     6121 aacggcgaga atgaattata gtaataatat atactttttc taggtgtatc tcatagacaa
     6181 tacgataatt gcggcagaat gtttctaatg tgccatcttc tctacgtcgc cctattgtag
     6241 gcataaatgc aatgagatcg agtttatttt gtaattgttc ccaaagtgaa actgcgctca
     6301 accaatgccc tgtataatct tctaccgact gtaaaatctc aatgacatta gaccttgcgg
     6361 taagtgacag ctcccaagat ttaggcataa ttaacctcaa acaaattttc attttctcgt
     6421 aattgctgac gtttctgcat atccgcaaat aaatgattta attcgttatt gatttgttct
     6481 ttggtaaagg tttctcctcg agctattgct tcttgaccag ctttaatttg ctctgcaaga
     6541 aactcatcat atccttgttc tttgaatagc atattctgct cctttgattt gtgtcttgat
     6601 tccactatgt tcaagacata gtttaagttt aaatctacgc tcaaagcaat gaagagtcaa
     6661 gggaatcctt atgttgatag ggtgtgcata tttttcgagt caaatgaatt aaaaatcatc
     6721 attacgatca cccactcata gattttatct tcctatctaa ggtcatcttc ttatatcaaa
     6781 caagcggtct aaatttgcaa aaaaatgaca aatttagacc gcttgtgagt tacaaaaata
     6841 taaactaatt gagcttatca attactgcgc ataactaaat actttcacca ctttggttac
     6901 tccacctaca ttgcgagcgg cttcggcggc ggcatcagct tggttttggc tgactttgcc
     6961 cattaaaaac acttcgccgt tttcggtgac gactttcact tcggtggctt ttacctcgct
     7021 atttactaac agtttggatt tcacttgggt ggtaatccag ctgtcttgtg taatctgtcc
     7081 aaagccgatt ttttcgcctt ggcggatttc gttgtaaatt tccgtcacgc cctccgcccc
     7141 agcagcgagg tttttcgcat tttccactac gtccatactt ggtgcttgcc cgattaataa
     7201 cactttgccg ttgtaagcca ccacgttaat gcgggcttct tgtttgattt gctcgtcttt
     7261 attaatgttg taagcgactt tttcttctaa aatttcatca tcaacttgcg tgccggtggt
     7321 gcgtgggtct gtcgccactt tagttgcaac cgccgcagaa gtcaccaccg ccgtggtgat
     7381 acaaccttgt aaagagataa aacctaatac taataggctg ccctttaata gtttatttgc
     7441 taatttgttc ataatctctc cttacgaaaa tagtaaatgg tcgataagtt cacataataa
     7501 attcacataa aaatgatggc tttcaatcac cctcagttga ttggttgaag gggctgaaat
     7561 ttcaatatca ctttcatcta ataagccgag tgtgtggtcg ttattactgc ctgttaaagc
     7621 aataatatct aaatctaaat gttgattttt cgccgtatga atagcttgca atatacattc
     7681 ttcattaccc acaggcgaaa aaacaatgaa gagatcaccc tcttttgcaa ccgcctgcaa
     7741 ctgtttttta taaacagcct ctaactcgtt atcttgtgcc acaactcctg ccaatacacc
     7801 gcttaattgc agttgtacgg cagcaaaact cggtcttgcc agttcataac ggtgaagtaa
     7861 gtggctgacc aataattcgg cattaccgta agagcgaccg tgtccgcaga cgataacttt
     7921 atcgccacgc aacaaacagg caacaatgcg gtttgccgct tcacttaatt tagccggcaa
     7981 tagctcagag gcggaaattt gggtttgaat actttcggta aatctatctt ggattttatt
     8041 taacattctt tctaacctaa aaagttggga atccacacag gcgtttggtt tccttcaaaa
     8101 gcaaccacat caaaacggca atttgcagtt tctaaacttt ggttttgttt aagcaaccac
     8161 atattagcgg cattttgcca tttttgttgc ttgcggtaat caatactctc aacggctgaa
     8221 ccgaagcggt tgcttttacg ctggcgaact tctacaaaca caacggtttg tccgtcttgc
     8281 ataatcggat ccagttcgcc acctttaaat gcttggttag cggcaacaaa acgaagccct
     8341 tgctgttcta aaaacactcg ggctttttgt tcataaaacg agccttgttc ccgtttatta
     8401 ataagcttgt tgaatagctc cattgcgata ttgcatccaa gataaactac gttcaatatt
     8461 acaatgttgt ccggctttta actcaccggt taaacctgaa attttgtagc ccggaatatg
     8521 gcggaattca ttgaatttgg ttgccaatga ccacgcatca gcccccattg cgtataaacg
     8581 catcattgag aaatcgctat tggctaaatt ctcggctttt ttatattcac tagaactttg
     8641 atcagctaac agtggcactt cactaaattt taccccttcc attgaagtgt aaaaatcaat
     8701 gccgttattt ggcgaattac tgcgagatga ggtatagatc gctaaacgat cttttaaggt
     8761 tgagctatca ataccttgtt taatttctaa tacttgctcg gcattcccca aaatatataa
     8821 agctgctttg ctcacacctg cattttgaat agcggcaacg gcatcaagcg gttggttgta
     8881 gtaacgcaca tcagcatcgc tattggtaag ctgacgccag cgtttggcaa aggcttctgc
     8941 cgagcggttg ccgtagtcat cttgaggggc cgcaacgata gcgacactat taccgtcttg
     9001 ataaattttt tccgcccctg attgtgcctc agattcagga gataaaccgt agtaacacac
     9061 ctgagcaata ttacgcacat tttcagtggc attcagtgcc aaaatattca cgttttggat
     9121 ttctgggcta agtagcattt catcaacacg agatttgagc aatggcccga caatcacatt
     9181 tgccccttgc tgttttgcct gtgaaattaa ggaattaaca tcagatgaat cggtatcaaa
     9241 agtttgcact gcggttgagt catcagcctt agcatcatta aaaccacgct tgataatatc
     9301 acccaaaatt tttgcatcac cacttagagg caatagcaat gcgacactgt ttaactgggt
     9361 ttgttggaaa ttactcaccc cttgtaattc cgttggcatt acggcaaccg cactatggct
     9421 cgggtattgg gctttccaat tattaattgc ttgtggcatt tgtgccggtg tcgtcacatt
     9481 ttgattatag atattaatta atgccaacca accagcaagc tccatctcac ccgcttcagg
     9541 cacagttttt tccagcattc ctcgatttgc atcacgcaag gtttgccaaa taatatcgtt
     9601 attttcttgg cgttcacggt tcattttata gaaattgtta agttgcgatc ttgcacgcac
     9661 aatgccaatc acatctttct gattggcagc aatacgggct tgggtttgaa tataacgcaa
     9721 acgctgtgcc gaacttaata gcggatcttg aatggctttt aattggcttg ttgcttgctc
     9781 gtttttccct tgtaaggcgg caagctgggc ggaaaccaag gcatactcta acttttgcac
     9841 ctcgttcatc tctgcggtca gaatttcact aaaggtattt tgagcctcat attccttatt
     9901 ttcatcaatt aatttgcgta cagctaaaag ttgatacgaa attttatctt ccgcttcttt
     9961 ggtttgatct gctttattaa tatagaattc cgagcttgaa taggcttcat ttttaatgga
    10021 ttcggtgaca ggatttaccg atgtacaggc agaaatgaat aatgcaatcg ccgttggcac
    10081 caatgccttt ttcattgtat ggtttaaaat agttgccatt gtcagatcct caatagaata
    10141 taatgtgcta aatttaatgt gaaatcttac ttatcaaacg agctaaaagc aaatgaaaaa
    10201 cagacaaaat tcatctggaa tcaacccaaa ttacggaatt ttatatattg ttgccacgcc
    10261 tatcggcaat ttaggcgata ttacccaacg ggcgttagac accctttcat ctgtcgattt
    10321 agtcgccgcc gaagacactc gccacagcgg tttattgctg agccactacg gcattaaaaa
    10381 accgtttttc gccttgcacg accataatga acagcagaaa gcggtcgttt tagtcgaaaa
    10441 attagcaaaa ggggaaaaca ttgcgttaat ttccgatgcc ggaacgccac tgattagcga
    10501 cccgggcttt catcttgtcc gtcattgccg ccaagccggg gtaaaagttg tgccgatccc
    10561 cggggcttgt gccgctgtta ctgccttatg tgcttcaggc atagcttcag accgtttttg
    10621 ttttgaaggg ttcttaccgg caaaaacaaa atcccgttgc gataaactta ccgagcttga
    10681 aaacgagcct cgcacactga ttttttatga atccacccac cgtattttag acactcttgc
    10741 cgatatgcag actgtttttg gcgaagatcg ctatgtggta atggcgcgtg aaatcacgaa
    10801 aacgtgggaa accatttatg gagatcaact gggtaactta attgaatggc taaatgagga
    10861 tagcaaccga attaaaggcg aaattgttct ggtggttgaa ggtaaaccgc aaagtagtga
    10921 ggaagccttt tccagccaag cgatcaaatt actgacatta ctctgccaag aacttccgct
    10981 taaaaaagcc gctgccattg tggcagaaac cttcggctat aagaaaaatg ccctgtatca
    11041 atatggattg gagcattttg agtaacagac aagcggtcag attttgtgag ttttttgcag
    11101 attggcactc ccctctctct gaaaaatgtt tctgaacgaa acatttcccg gtctcaatac
    11161 tcgctacgct cgctccttcg gagccgttgg caaagccaac gttcaaatgt aaacatttgt
    11221 cccataaggg gagagagcta aattattcgc ttgtcgaccc ctctccactc gtgggagagg
    11281 gacagattct tcttcgttca gaagaaaaat cagggagagg gggaagtttc tcgcactaaa
    11341 ttccgataag catatgccat aatcgcaaac atcaacagca agtaaagcgg taataattga
    11401 atgctgatag cattagcaat aaagccgaaa atcggtggca ataacaggac gccgatatag
    11461 gcacttgcca tttgcacgcc gatcaccgat tgagacttgt cctcgccgaa aaacgcaggg
    11521 gtcgaatgaa ttaagcacgg gaaaatcggg gcacaaccta gcccgataat aattaaactt
    11581 gccagcgtaa catatttgcc taacggcaag cacagtatcg caatgccgac taagatcaag
    11641 gctgaaccaa gtttaatcat ttgtctgttg ctaaatttaa gcgtcaagaa gccacttacg
    11701 cctctgccaa cggtaatgcc gataaagaaa atactgccga acgcagccgc ttgttctaag
    11761 ccgacatctt taaccattac aaaatagcta ctcgcccaca gtccggcggt ttgttctacg
    11821 ccacaatagc caaaaaaagc taaaatgcca tatttcacgc ccggaatttt tagcgtttct
    11881 tttaagctca tcggttttgc cgaatggttg atttcagcgg taatctgctt gcgtaatttc
    11941 caaaaaggca gtgagcaaaa cagcattagc gttagcccga tttgtaacat tgaaatggtg
    12001 aaatagcctg cctcccagcc tcgcccttgc aataaggcgt agcctaaaat gtgcggcccg
    12061 atagaagctc caacgcccca catacaatgc agccagctca tatggcggct tgcgtagtga
    12121 actgctacat agttattcaa cgccgcatca accgctccag cccctaagcc gtaaggaatt
    12181 gcccataaac ataagatcca gaaagcatca ctgattgaaa agccccacaa tgctaaagcc
    12241 gttaaggcaa cgctgatgac cattactttg cctgtgccga gatggtaggt cagtttgtcc
    12301 gacattaagc tagaggtaat agtgccaatc gacataatca tcgagaccac accggcatag
    12361 gaaagcggaa cagcaagctg ttggtgcatt gacggccacg ccgagcctaa aattgcatca
    12421 ggcaacccaa ggctgataaa agagagataa ataatagcga gtaggaggtt agtcatagct
    12481 tacaatcggt cacaaaaagc atcgcttttt gaacgttggc tctgccaacg ggagcgaagc
    12541 tgtgaacaat ccttacaagg attgtgaata atttttgata aattttttgt aaaaaaatgg
    12601 cttaaggaga ccttaagcca aattttatta tttcacttct gccaaatagg ccttattagc
    12661 acggtcaaag gtgtctaata cggctttgct tggcattggc gtgataagtg ataccacaat
    12721 gatcattagg gtagcagcaa taaagcccgg gatcatcgag taaatttccg gtaaggcgag
    12781 cttaagtgta atatcgcccc aaaaatgcac gattaacgca ccggtcagca ttccggcaat
    12841 cgccccaaaa ccgttcatac gtttccagaa tagtgaaagc aataccacag gcccaaatgc
    12901 cgagccgaaa cctgcccacg cattacgcac taagccaaat actttgctgt ccgggtcttg
    12961 ggcaatataa atagctaatg ctgccaccgc aagcaccatc aagcggctta accaaaccag
    13021 ttctttatcg gaggcttgag ggcgtaaaat gcctttgtat aaatcttcgg taatcgcact
    13081 tgaacaaatc agtaattgag cggaaagggt actcattacc gcagcaagaa tggcagataa
    13141 taaaatgccg gctacccaag ggttaaataa caatttagct aattcaatga aaatttgttc
    13201 cggatttttg ctgatattgc cgacttgctc cgggttgcca aagaaatatg ccataccaaa
    13261 ataaccaatg cctattgccc ccagtaagca gataatcatc caagtcatac tgatacggcg
    13321 tgcattttca agagacttca cactttctgc cgccataaaa cgcaccacaa tatgcggttg
    13381 tccgaaataa cccagccccc acgccattaa gctgataatg cctaatacgc tggtgccggt
    13441 gaaaatgtcg ttgtagtctt tttgtgccat ctcgcctgca cggtcgatta aggcttgcat
    13501 ttcatcaaaa ccacctaagt tatacatcac aaaaaacggg gtgagaatca gggcaaaaac
    13561 cattaatgaa gcttgaatag tatccgtcca acttactgct aagaaaccac caataaaggt
    13621 gtaaagaatg gtagcaacag ccccatacca aattgcttgg tggtaaggta agccaaataa
    13681 gttttcaaat accctcgcac cggccacaac gcctgatgca cagtaaattg caaaaaagag
    13741 taaaaaaatg accgctgata aaatcttaat taaacgggtt ttatcgccaa aacggttatg
    13801 aaaatattcg ggtaaggtta aggcatcgcc actgtgttct gtatgcgagc gtaatctgcc
    13861 tgcgactaat ttccagttta gataagcacc gatagttaaa ccgattgcga tccagccttc
    13921 gatcaaaccg cctgcataaa cggctccggg taatcccatc agcaaccagc cggacatatc
    13981 tgatgcaccg gcagaaagtg cggtcacaaa gctacctaaa cggcgaccgc ctaaaatata
    14041 gtctgaaagg ttattggtaa cacgataggc aataaagccg atggcaatca ttcctagaat
    14101 atagacaaca aacataattg ttgttggatc aagaccaaac attattaatg aatcctcaag
    14161 atttcttatt gtataaagat taaagtgagt aaagggtaaa agattacctg aaataatcaa
    14221 aatggcaaca caaaattacc gtccgcccag ctcagctgaa attttttctg ctgtttcttg
    14281 aatagccggt agtagatttt cccgccccag ctgatttaaa cgagcagttg aaagcgaaac
    14341 agaaatcgca taatggactt tttgttggtg atcaaaaatc gggcaagcaa tacaagaaac
    14401 ccctaattca ttttcttcgt tatccattgc aaaaccttga cggcgaatgg tttccagctc
    14461 ggtttgcatt ccgtcaatcg tggtgatggt attgaccgtc agtttttgaa tgatgtccga
    14521 tttagtctgc caatattgtg gaatgtaatc tgccgaacca taagcaagaa acagttttcc
    14581 cattcccgag caatagagtt gcatttgttg cccgatatag gctcgggtac gcatcattcc
    14641 ggtggtagct tctaatttat tgattaaaat agcgtgatcg ttttcccgca tcgcaaaatt
    14701 taccgtctcg cctaaggtta aatttagggc ttctaaatgt ggggcggcaa tatgcaaaat
    14761 gttaatcgaa cttaaggctc gctgcccgat tgccaaacat ttggtggtta aacgataact
    14821 gcccgcagtc ggtgcagggc ggacgtagcc ctcttgttgt aagccttgta gcagacggtg
    14881 aaccgtgctt ttgttcagcc ccgaaagctc ggctaaatgg gcaagcggac agccgtttgg
    14941 gaatttcgct aacaattcca atagtgttaa gccacgaatt aaactttgat ttccactcga
    15001 ttccttttct ttctttgcat tcatttggct gctcccaaaa taatatgatc taacggcata
    15061 tcccaactct caatcggtaa ttgttcgact tgctgacatt cgtgtgccaa accgacacta
    15121 atcagatgag gtgcttgagc aagggttcta tcataaaagc cacctcccat tcccagccga
    15181 tttccggctt tatcacaagc aacaagtggg gtaaaaatca tctctaattc ttgcaacgga
    15241 aggatatttc taacatctaa tatcggctct tgaatcccca agcggttaaa ttttagaata
    15301 gttttgcaat tgtaatttac aaacaataat tgaccgcttg taaaagggtg caacagcggc
    15361 agatacagct ttttgccctg ttgaagtaat tgttccatta aaggaatagg agaaatttca
    15421 ccgttaaaag gcaaataaaa ggcaatatgt gagacttggt aagattcaat caatgaaaga
    15481 gcttgaggaa taatggattc ggctgcttga gtttgctgac cggcagttaa actttgccgt
    15541 ttaagccgca ttgattgccg taattggcta cgaagatcgg ttaaagatga ttgattcata
    15601 taaagacccg agatgccgct accagtaatg gtccttgaac cagtcggttc aagggagtca
    15661 aacttaactg cttttaggct tcttactcct aattaaatta gggtaagctt gctcaccagc
    15721 gttagatgtc caactcaaag tttcaaatta tcggctcagg gacgtaactc gtaacgcaca
    15781 ccccaggcaa gacttatagt aacggaattt gatgattttt cctagtattt aatcccacaa
    15841 aatgcaaatt ctcaatcttt ttggctctat tcctaatcaa ttcgattaaa aattacctaa
    15901 ttggattgaa atttgccaag cgaattatct aactgctcaa tacaagccac caacacattt
    15961 ttattttctg cattttggcg ttgggcttgc tccagttcat aatttaaatt cagtgccaca
    16021 atcgctaaca ctttttcaag ctgaataatg ccggactgct ctttcaatac caccacacgc
    16081 tcttccaaac gctgtgctga agccagtaaa tgctcttgct gttcaacagg gcattgcaag
    16141 cgtaaaacct gcccaaaaat ctgtagttcg atattatttg ccgacataaa tacgaaatcc
    16201 tcacttaatc gcaaaatttt tgctatatta tgccattctt tagcaaaatc acaaataatc
    16261 ccattatggc tatcacagat tcccaacaat ttaaacaact ggtatcacaa ttacaaattg
    16321 atgatacacc ggcagagttt cacggttttt tatgcggttt aattgccggc ggtattcaag
    16381 atgagtcttg gaaaaccctc acctaccaat ttaccaatga cggacacgca ttttccatcg
    16441 agccgttaaa cactttaacc gatttttatc agcaattaag tgaaagcttt tcagaagcca
    16501 acacgctgtt tagcctttgg ttgccacaaa acgaagaaga tggttttgcc ttagctgatg
    16561 gtatctccga atggacaaac agtttcttac ttggacttgg cgttgctcaa cctaaattac
    16621 aacaagaaac cgatgaggtt ggcgaggcaa tcgatgattt agatgaaatc gccaaactag
    16681 gctacagtga cgatgataat aacgaggaac tactggaggc tggcgaagaa gtgttggaat
    16741 atctgcgagt tttagcctta ttcttgcaca gccattttgc tttaggcgtg ccgaaaaccg
    16801 aagaaaagcc accattacac taatgaataa atacaagcgg ttcaatttca tctctatttt
    16861 gcaaatatat tatgcggggc gaaaaatatt tcgcccctac caaaatcaaa ggaaaaacca
    16921 atgtcagaat taaaaaacga tcgctattta aaagcgttat tgcgtgaacc tgtggatatg
    16981 accccggtct ggatgatgcg tcaagccggg cgttatttgc cggaatataa agccacccga
    17041 gcagaagccg gcgattttat gtcactctgc cgtaacgctg atttagcgtg tgaagtcacc
    17101 ttacaaccgc tacgccgtta tgaactcgat gcagctatcc tattttccga tattttaact
    17161 gtgcctgatg caatgggatt aggtttaagt tttggtgcag gtgaaggccc taaatttgcc
    17221 cgcccggtgg caagcctcaa tgatgtcaat cagctaccga ttcctgatcc ggaagacgag
    17281 ttgcaatatg taatgaatgc ggtgcgtacc attcgccgag aattgaaagg cgaagtgcca
    17341 ctaattggtt tttctggcag cccttggaca ctcgccacct atatggtgga aggtggttca
    17401 agcaaagcat ttaccaaaat caaaaaaatg ctttacgctg agcctcacat tctacacaaa
    17461 ttgctggaca agttagctga cagtgtcatc ttatacttaa atgcccaaat caaagccggt
    17521 gctcaagcgg tgatggtatt cgacacttgg ggcggtgtat tggctcacga ggattacaaa
    17581 gccttctcat tacactatat gcacaaaatt gtggacggct taattcgaga aaatgaagga
    17641 cgtaaagtgc ctgttaccct attcaccaaa ggcggaggct tatggttaga agcgattgca
    17701 gaaacgggct gcgatgccgt tgggctagat tggacagtcg atattgccga tgctcgccgc
    17761 cgtattggcg acaaagttgc gttgcaaggc aatatggatc caagcgtgct gtatgcaagc
    17821 cctgaacgaa tcgaacaaga agtaaaaaac attcttgccg gcttcggcaa cggcagcgga
    17881 cacgtcttta atcttggaca cggcattcat ttagatgtgc cggtggaaag cccgaaagtg
    17941 tttgttgatg cgattcacaa ctactcgaag cagtatcata aataatggat aaaatgccct
    18001 ctctccttgt gggagaggga cagatttttc tttgttcaga agaaaaatca gggagagggg
    18061 caagcggtca tttttataca gaattttgca aaatcgttcc cttctctctg aaaaataaag
    18121 aaggaaatac aatgagaaac ccgattcata aacgcatgga acgtttcgag ccttggcaaa
    18181 acaccacatt tatggcgtgc ctatgcgaaa gaatgtaccc caactatcag cttttttgtg
    18241 aagtaaccga acagcctgaa aaagctaagg tattccaaaa tattttaaat ttagtctggg
    18301 aatatttaac cataaaaggg gcaaaaatta atttcgataa ccaactggaa aaattagaag
    18361 aaatcattcc tgatgtgaac gaatatgcgt tttttggtgt cttacctgca caggaagctt
    18421 gcgaagccct ttctgaactt cttcacagta ttatcgctgg ttcaaccctt gaacaagcaa
    18481 tccgcatcag ccaaatttca ttaggcacag ttgccagcta tttggaaatg caacaagata
    18541 aagaactgaa cgagcaagag ctaaagaatt ctgaagaaat tcaagaagaa ctggacgtac
    18601 agtggcaaat ataccgcttg ctaaacgaat gtgaaaaacg tgatcttgat ctcatttttg
    18661 aacttaaaga tgaaatcaga agcagcggga ttagtaatat cgggttaaat attaaccaat
    18721 agcattgata tttgcaaaat cttgcttaat aatactttga gaaatcttaa ctttgaatta
    18781 agctatcgca aggtttcaat aacttctcag acgttttacg tcatttatta aaaacacata
    18841 tcttagagga taaaactatg aacaaaactg agttaatcga tgctatcgcc gctggtgcaa
    18901 acttaaccaa gaaagatgct aaagcagcat tagaagcaac tcttgatgca atctctgcaa
    18961 gcttaaaagc aggcgacgct gttcaattaa tcggcttcgg tacttttaaa gtaaatgagc
    19021 gtaaagcacg tactggtcgt aacccaaaaa ctggtgaaga gatcaaaatt gcagcagcta
    19081 aagtacctgc attcgtttca ggtaaagcat taaaagattt agtaaaataa ttctttaaag
    19141 atataaaaat aaagcctgga ttataatcca ggctttattt ttagcgtgtt tcgatagcta
    19201 cagagaaact aaaaagcact atttgctttc aatcgctttt ttcatttctt caacttttgt
    19261 ttctgtctca actttcactt ctgctgcctt ctcagctact gcctttttag cttcttctgc
    19321 tttatctgct gttgcttgag tcgcatcact tgctgctgct tttagttctt ccactttagc
    19381 ttcagttgtg gcttttaact cagcagcttt ctctgctact gccttttttg cctcttctgt
    19441 tttatctgtt gttgcttgag ctgcatcatt tgctgctgct tttagttctt ccactttagc
    19501 ttcagttgtg gtttttaact cagtggcttt ctctgccact gccttttttg cctcttctgt
    19561 tttatctgct gttgcttgag ctgcatcatt tgctgccgct ttcaactctt caaccttcgc
    19621 ttcagtagcc gatttaactt ctgcagcttt ttcagctaca gcttctttta cttcaaccgc
    19681 tttgtctgta gttgattgcg cggcttcttt tgcctgatca caagcagcaa gtgccatagt
    19741 tgtacctaac actaataatg ataaaaatga ttttttcata ccgagttcct taaataaact
    19801 aaaaataacg cactttgtaa taaagtgcgt ttacttaggc agaaaaattg atagaaagtt
    19861 catactttga taaaaaaatg cctattagtt ataactaata ggcatttaaa ccaattaatt
    19921 gtacttaacc attggttgaa cgaggcgctg actgcacttg gctcactgcg ctggtatatc
    19981 cgccaaaccc tttgccatag aaatagtatt tagagttttg ccattggcta atttcgacta
    20041 cttcaagctt gtcacttggt gcagaagcaa tggtcatagc ggcttgggta tgctcaacac
    20101 gagagaatgt acctaatctg ccgttaaatc gataatcgct ttcgtaagtt gattttggca
    20161 cttcaatcgg gttagtaacc tcttccacaa tcgctggtgc aaccgttgct tgcgattgga
    20221 atttactgac tcgctcattt ttctctttaa ctaaatcacg ttcagaatgc tgagcaacaa
    20281 cactaccaaa tggagcaata tgctctttta cctctactgg ttttacgtct tcagaagcaa
    20341 ctttttcttc tttcggtttg gaaagctgtt cttgcttctc cttaaccaaa tcacggttag
    20401 ttgcttgtgc tacaaagcca cctagcggac taactgtatg aagcttcttc tcttctgatt
    20461 ttttctctgt ctcaacaagc tgattttctt gctgttcaag catttgatca acagataaga
    20521 atgatgcttt ttcctctact ttgacagtag aggtaacctg aacccaaact ttaccgctcg
    20581 caagctcagg cgaagcaaca gctgaagtta gaggcatagg agaaataacc tgattgcgtt
    20641 gattttcacg acgacgctga ttgcccacac gcaaatggcg aggtaaacgg cgttggcggt
    20701 tattttcacg tccttcgttc gttgtttcta cctcaggagc tgtttcaaca ggtacttctt
    20761 gggttgcaag cggttgaatt tgttgatttt tttgcacatt ttcaacttgt tttgccaccg
    20821 tttcctcaac agcaggtgtt agattttcca cttctacttc ttgtgtttcg atacgcactt
    20881 ttttacgtag atcacggcgt tggcgacgtt cagtttgaac ctcacgggag ttgctacgac
    20941 tctcttgctc ggttacaccg gaaacttctt ccactaccgc tcggcgtgct tttttctcgc
    21001 tacgcatcgg tttttcttcc gctggagtag tttgagcttg cggagtttta cgctcttgat
    21061 tttccgcttt agcattacgg ttcgggcgtt cacggcgttg gcgacgctca cgctgattgc
    21121 gtgctttcgg cttcggtttt tcttccactt tttcttcact aaaccagcct ttaatggtcg
    21181 caattaagcg gcttaacagc gatggttttg gtggttcggt tggtgttggc gaaggttgga
    21241 gattcagctc cgagctttgt aacactgact ctaccgtaat caccggctcg ttacgggtaa
    21301 ccagcacatc ttcttgatgg tgatgatgtg ggtgttcttc ttcatgacga gcttcgtaat
    21361 gcttggttaa atcgtagctt aacgttggag taatttcatt ttcacgcagg cgatatacgc
    21421 tgaaatgcgg cgtttccata ctttcagacg gtactacaac aatttgtaca tcgtggcgtt
    21481 tttcaatgct ggaaatcgct ttacgttttt cgttgagtaa gtagctggcg atttgcaccg
    21541 gcacaatggt gtgaacctgt gcagtgtttt ctttgattgc ctcttcttct aataaacgta
    21601 agattgacag tgcgattgat tcattatcac gtactttacc agttccctga cagcgagggc
    21661 agatatgcga tgaagcctca cttaatgatg ggcttaaacg ctgacggctc atttctaaca
    21721 aaccgaaacg ggaaatacgg gcaaattgga ttctcgcacg gtcttggcgt gtcgcctcac
    21781 gaatacggtt ttctacttca cgttggtggc gaaccggtgt catatcgata aagtcgatca
    21841 caatcaaacc acctaagtca cgcaaacgta actgacgggc aatttcatct gccgcttcta
    21901 agttggtgtt aagagcggtt tcttcaatat cgccgccacg ggttgaacgt gaggagttaa
    21961 tatcaattgc tgttaaggct tctgttacat caataacgat agagccaccg gaaggtaaac
    22021 gcacttcacg ttggaatgcc gattcgattt gcgattcaat ttgatagtgg ctgaatagtg
    22081 gtacttcgcc ttcgtataat ttcacacggt tgatgaaatc cgggcgaaca aggcgaatgt
    22141 ggtttttggc tttctcgaac acttttttgt tgtcgattaa gatttcgcca atatcacggc
    22201 gtagatagtc acgaattgca cgcacaatca cgtcactttc ttggtgaatt aagaacggag
    22261 ctgggcgaga ttccgccgct tttttaatcg cttcccagtg gtgtaacaag acttttaagt
    22321 cccattgtaa ctcttccggc gatttgccga caccggcggt acgaacgatt aagcccacgt
    22381 cttccggtac atctaagcag tctaaagcct ctttgagttc taaacgctca tcaccttcaa
    22441 tacggcggga aataccaccg gcacgagggt tgttcggcat taagactaag taactgcccg
    22501 caagcgaaat aaaggtggtt aaggctgctc ctttgttacc acgctcttct ttgctgactt
    22561 gaacaatcac ttcttgccct tcttgaataa tatctttgat attcgggcga ccgttaaaca
    22621 catagtctga cgggaagtat tctcgggaaa tttcttttaa aggtaggaag ccgtggcgtt
    22681 ctgcaccgta atctacgaat gcagcttcaa ggcttggttc gacacgggta attttaccct
    22741 tgtagatatt ggattttttc tgttcgtgac ccgggctttc aatgtccaag tcaaataagc
    22801 gttgcccatc aacgagggca acacgcaact cttctttttg agttgcattg attaacattc
    22861 ttttcattgt gttttctcat tattttgttt ttgtcttaca cggtcatttt gacccattaa
    22921 taagggaatg gatttaatgt ctatctcacg actgtcttca ttcccgatag tgcattgatg
    22981 gttgtcttac gtttactttg tcttgtctta aagaaactgc aaaatcaatc acttaaggct
    23041 tgtttatctc tattacaagc ggttattttt gctttatttt ttgcaatttt ctattgcttt
    23101 ttcaagccaa aaataacgag tcattatcgc attttaaccc tgaaaaaggc aaggtaattg
    23161 ttgtatggta gctgttattt gttgtggaga tgaatataat gaaaatgaat ggggtgataa
    23221 ggaaaaataa tgagatttag tgtgagtgct ggagataagc gtgaaatcca agcggttaaa
    23281 tttgcaaaaa aagagctaaa tttaaccgct tgtaaagcga atattatgct tttgccgtca
    23341 tttttttcac tgcttcttca gtggtccaga ggtcatttgc gataatacgg tagttggtta
    23401 atgctgcaag gtagccgtca ccttcaggaa tacgaggccc tgcggttgca tctttcacca
    23461 ctgttacttc aaaaccttgt tcgattaatt cacgcaagtg tgaatcaata cataagttgg
    23521 cagccatacc ggctaaaatt acttggtcga ttttacgttt gcgtaattgc aacactaagt
    23581 cattggtttc cggtccaaag attttgtgtg gagaggtaat cactgttttg ccatcaaaaa
    23641 tgtatggttt gtactcaggc ataaagtctg cacctgaatt ttcaaagcct tccatggtgt
    23701 attgaccttt tctcgcaaac attccgctgt cgtgcataaa gtgttcaccc ggtgaaccaa
    23761 attcccattt gtgatcagtc gggtaatagt agtgtggcga aacaaaggtt ggcatatcga
    23821 ctgcttttgc tgctttaaat aagctttcaa tgttagctac agtgttgttt tctttaattg
    23881 agtcacctaa cacgccccac atcacgcctt tcgggcctag gaagtcaatt tgtggatcaa
    23941 ctaccactaa tgccacacgt tttggatcaa tggtcattcc atggcgtttt aaaccgtatt
    24001 gttccggctc ggcataaggt gatttttctg cactggcttg ttttgcattt gctgttgatg
    24061 caatacctaa tgcacctaat acactggctg ccattgcaat acgagagccg tggttaaggg
    24121 cttgacggcg ttcttggttg atttcttcaa attgttcctg tgacatcatt ttatcctcat
    24181 taatagatat acaaattgta atgataactt gcgttaccga acgggttgct attatagtga
    24241 tcgcatttct cctgttttaa ccgttcgtct caattcttta cccaaggagc ttaatgtgga
    24301 tagtttgact catcttttta cccaatttca gtttcgcacg gatttatttc atctaggtca
    24361 gttatgccaa atcgggagtt ttaatgaaga gaataaaggc tatttacatt tcgttcgtca
    24421 gggacgtttt attttaaatc aggctgccga acgcccgatt acgattgaac gcccagcgat
    24481 tattttttca ccaaccaata ttttgcatag tttgcacccg ttagatgata gtgggttaga
    24541 tatattttgt attaattttg attttggcaa agggattcgc aacccattaa ctcatacctt
    24601 gcacaatatt gtgatcttag agttggcaca gtatccggaa ttgggggtat tagccgatca
    24661 aatttttagt gaagtgaatc agaaagggtg tggttatcag gcaatgattc atcatttatg
    24721 tgcctatttt accttgaaag ttgcccgcca atgtttggaa aaagggtata ttcaaacagg
    24781 tttgttaaaa ggcttggcag ataagcaact ggggaaagtg ctgttagcta ttcacaatgc
    24841 accagagcga gattggaatg tagaggagtt ggcaaatgtg gcattaatgt ctcgctcacg
    24901 ctttgctgct tattttaaac aaacgatggg ggtttcaccg atggattatc tcactaactg
    24961 gcgactagcg gttgcccagc atttattaca aaaagggata ccagtagcat tggtggcaga
    25021 gcaagtcggt tatagccata atgctgctct cagccgtgtc tttatgcggg aattagggct
    25081 ttcgcctacc gagtggctgg ctcaaaataa agagtcgtaa cctgatgaga aacaaatagc
    25141 cgaatggtaa aaatgtcaaa aaatttaacc gcttgttatg ttgcacaagc ggttaaatgt
    25201 tgccttaaat gccaagagaa tcccaaatct catcaatgcg tttcaccaca ttgtcatctt
    25261 tttcgatggg tgttccccat tctcgggtgg tttcacccgc ccatttattg gtggcatcaa
    25321 tccccatttt tgagcctaac cctgcaattg gcgaggcaaa atcgagataa tctatcggcg
    25381 tgtgttcaat cagtgtcgta tctcgagccg ggtcacaacg ggtggtgatc gcccaaatta
    25441 catctttcca atctcgagcg ttcacatcgt catcgcacac aatcacaaac ttggtgtaca
    25501 taaattgacg caggaacgac caaactccca tcatcacccg tttagcgtgt ccggcatatt
    25561 gttttttcat cgtcactacc gctaaacggt aagaacagcc ttcaggtggc aagtagaaat
    25621 cgacaatttc cggaaactgt ttttgcaaaa tcggaataaa gacttcattc aatgcttcac
    25681 ctaatactgc cggttcatca ggtggtctgc cagtgtaggt agaatgataa atcgcatctt
    25741 ttcgcatcgt aatatgggta acggtaaata ccggaaaata ctcttgctcg ttgtagtagc
    25801 cggtgtggtc gccataaggg ccttccaatg cggtttcttc cagatcgata tagccctcca
    25861 gcacaatttc gctacttgcc ggcacttcta actcattgga aatagatttg accacttcgg
    25921 ttttatgccc tcgcaacagc cctgcgaacg catattccga aagcgtatcc ggcacagggg
    25981 tgactgccgc taaaatagtg gctggatctg caccaatcgc aaccgacacc agaaacggtt
    26041 tacccgggtt ttcggctttc cactcttgga agtctaacgc accgccacgg tgggaaagcc
    26101 aacgcataat cagtttgttt ttgccgataa gttgctgacg ataaatgcct aaattctggc
    26161 gttttttgta aggcccttgc gtaatcgtca gcccccaagt gaccagtgga gcgacatcgc
    26221 ccggccaaca gtgcataatc ggtaatttgt agagatcaac ctcatcaccc gaaatcacca
    26281 cttgctgaca atcggctttg ctcagtacct taggcggcat atttaaaatt tgcttccatt
    26341 gtggcagttg cccgatcaaa tctttaaacc ctttcggtgg ttcaggctct tttaaaaagg
    26401 caagtaactt gcccagctca cgcagagctt tggtatcttc ttgccccatt cccagagcta
    26461 cccgtttggg cgtgccgaat aaattacaca acaccgacat ttgatagcct ttcgggtttt
    26521 caaacagtaa tgccggcccg cctttgcgta aggtgcggtc ggcgatttct gtcatttcta
    26581 aataaggatc gatttcttgc tgaatacgga ctaattcacc ctgagactca aggaaatcga
    26641 gaaattcacg gagattttta tacttcatag cggtcatatt tttcgat
//
LOCUS       DANLUA010000011        25108 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLUA010000011 DANLUA010000000
VERSION     DANLUA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25108
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            29..2298
                     /locus_tag="PXB44_000400"
                     /pseudo
     CDS             29..2298
                     /locus_tag="PXB44_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2376..3077)
                     /locus_tag="PXB44_000401"
     CDS             complement(2376..3077)
                     /locus_tag="PXB44_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL5280916.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(3082..4491)
                     /locus_tag="PXB44_000402"
     CDS             complement(3082..4491)
                     /locus_tag="PXB44_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL5280917.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(4493..5224)
                     /locus_tag="PXB44_000403"
     CDS             complement(4493..5224)
                     /locus_tag="PXB44_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL5280918.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(5368..6972)
                     /locus_tag="PXB44_000404"
     CDS             complement(5368..6972)
                     /locus_tag="PXB44_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL5280919.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(7262..8353)
                     /gene="ychF"
                     /locus_tag="PXB44_000405"
     CDS             complement(7262..8353)
                     /gene="ychF"
                     /locus_tag="PXB44_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL5280920.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
     gene            complement(8539..9126)
                     /gene="pth"
                     /locus_tag="PXB44_000406"
     CDS             complement(8539..9126)
                     /gene="pth"
                     /locus_tag="PXB44_000406"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL5280921.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            complement(9199..10074)
                     /locus_tag="PXB44_000407"
     CDS             complement(9199..10074)
                     /locus_tag="PXB44_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL5280922.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            10296..11876
                     /gene="prfC"
                     /locus_tag="PXB44_000408"
     CDS             10296..11876
                     /gene="prfC"
                     /locus_tag="PXB44_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL5280923.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            12135..13709
                     /locus_tag="PXB44_000409"
     CDS             12135..13709
                     /locus_tag="PXB44_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5280924.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            13929..14861
                     /locus_tag="PXB44_000410"
     CDS             13929..14861
                     /locus_tag="PXB44_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5280925.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            14871..15653
                     /locus_tag="PXB44_000411"
     CDS             14871..15653
                     /locus_tag="PXB44_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5280926.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            15742..17154
                     /locus_tag="PXB44_000412"
     CDS             15742..17154
                     /locus_tag="PXB44_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5280927.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(17241..17984)
                     /gene="rlmB"
                     /locus_tag="PXB44_000413"
     CDS             complement(17241..17984)
                     /gene="rlmB"
                     /locus_tag="PXB44_000413"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL5280928.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(18039..19406)
                     /gene="pssA"
                     /locus_tag="PXB44_000414"
     CDS             complement(18039..19406)
                     /gene="pssA"
                     /locus_tag="PXB44_000414"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL5280929.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            complement(19476..21023)
                     /locus_tag="PXB44_000415"
     CDS             complement(19476..21023)
                     /locus_tag="PXB44_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL5280930.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            complement(21148..23964)
                     /gene="ileS"
                     /locus_tag="PXB44_000416"
     CDS             complement(21148..23964)
                     /gene="ileS"
                     /locus_tag="PXB44_000416"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL5280931.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            complement(24067..25029)
                     /gene="ribF"
                     /locus_tag="PXB44_000417"
     CDS             complement(24067..25029)
                     /gene="ribF"
                     /locus_tag="PXB44_000417"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL5280932.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
BASE COUNT         7158 a         5751 c         5016 g         7183 t
ORIGIN      
        1 gttaaagaaa aattatctca ctgtatcaat tctccttgcc attagtggag taggctacgc
       61 taatgaaatc tctttagaga ctattactgt tgatggaaat acgccttcaa ccaaagggaa
      121 attactaggt ggtgaactaa atagcaatga atctgttgtt gatgaaaaaa attaaaacaa
      181 ggtagtatta cattaggtaa cgctttatct ggtgaattag gcattcattc aagccagttc
      241 ggaggcggtg caagtacccc aattattcgt gggcaagaga gtaaacgtgc aaaaatctta
      301 caaaataatg gcgaaaactt agatatgtcg ggaatgtctc ctgatcatgc agtaaccgta
      361 gatgcgctac ttgctaaacg tattgaaatt ttacgtggtc caactacttt actttacagt
      421 gcaggtaata cggcaggtgt aattaatgtg gtagacaata aaattccaac cgctattccc
      481 gaaaaaggct atgaggggca atttggtgtg cgttttggct cagccagtaa agagcgttta
      541 acctatgcag gctcaacctt tgcccttgga aatcatttag ccttgcgggt acagggaatg
      601 tataacaaag cttccgaata ctatgctcca cactttacga ttgaaggcaa accttaccac
      661 cgcgtaccag atagcgatgt acaatcacaa accggcacag taagcctttc ttggattgga
      721 gagcgaggac atcttggtat tgcttacact gatcgccgtg ataaatatgg attaattggt
      781 cacacacaca aatacgatca ctatactatt agtattattc gccaagccgt tatgtttgca
      841 aaaggttatt tacgttttta tccgcacctt gcagaagaag gagatattga ctataacaac
      901 cctggtatcc gtttactgca tactcatatt ccgggaggct cacattatgg acaagatact
      961 cacgagcacg gcaaaccttg gattgatatg cactccaagc ggtatgatat tgatggaagt
     1021 ttgcaaaatc cacttcctgg ttttgaagaa gccaaaatat cagctaacta tgtagattac
     1081 tatcatgatg aaaaagatgg aaaaagagtt gaaaattact tcaagaacaa aggaaaaaat
     1141 ctacgtttcg aattggttca caaagagtgg aaagggctaa aaggagctat tggtgtgcaa
     1201 tataccaacc aatctacctc agcacttgca cttgaagctt cacgagctgc aaaagtattt
     1261 aataaacagc ctttactcaa taatccaaaa acaaaattat ggagtctctt tgcgattgaa
     1321 cgccttaatt taggtgactt tacctttgag ttaagtgggc gggcagaacg tcaaaaaata
     1381 gcaatggatt atgatgtgaa attaattgat cgctggctag gctttaatac acctatgcca
     1441 aacttggacc cacataaaga caaaggttat tcttatagct ttgcaactca ttggtatttc
     1501 gcaccaaacc ataagttaac attaaatgct gcacatcaag aacgtttacc taatgctcag
     1561 gaactctatg ctcatggtaa acacattgct ttaaatgcat ttgaggctgg caataaaaat
     1621 ctaaaaaaag agcgttctaa ccaaattgag ctatcattag cttatgttgg cgacaaatgg
     1681 gattataaat taaatctcta ccacacccgc tatggcaact atatttaccc attaactctt
     1741 aatgataatc gtggacctaa atcttttacc gatgaatata acttgaaagt gaatagatat
     1801 tatcaaggcg aagctcgttt tagtggggca gaaggcgaaa ttggttatct atttacacca
     1861 aattatcgct tagccgtatt cggagactat gtccgtggaa agttagtcaa cttacctaat
     1921 attgcaatga gttacaacat ttggacaggt gaagttgaca aatgggcttc tcagccagac
     1981 atttctgcac ctcgtatacc accactacgt ttaggagcaa gatttaatgc tgacttcaac
     2041 ctaaactggt ctggaatgtt agaatattac cgagtatttg ctcagaaaaa agtctcgaaa
     2101 tacgaacaag ttacccctgg ccatcatcaa gtaaaccttg gcgtaaccta tagtaatcat
     2161 tttaatcaaa cagaatacca agtattcctt aaagtggata acttgcttaa tcagaaaatg
     2221 taccaacatg cctcatatct accacatatt ccacaaatgg gtagaaatgc aatgcttgga
     2281 atgaatatca gtttttaatt tgtaaatctc tacaaaaata taaccgcttg taaatttcta
     2341 tctacaagcg gttactttca gtggattttt tgcaattaga gatcgtcaat taccaagtaa
     2401 attttattta ccgggccgtg aacgccaact actttaatca actcaatatc cgcagtcgcc
     2461 gatgggcctg agatgatatt cacacaagat ggcattcttt cacctcgttg ggctttttcg
     2521 tgcagaattt gtgcaagctg tgccacacga ggtaacactg tacttttgcg taacacaacc
     2581 accgattttt cgggcaataa gctcactgaa cgcccaaatt ctttatcgga aaacagtaca
     2641 ataccgccag actctgccaa gccatattca ccgtaaacca caccgatatt agcagttctg
     2701 gctttttcaa agttggtttc agcttggttg aaatcccaca catagctgtc ttcgtatttt
     2761 gcttgaatgg ctgcggtaat cccaagatcc tttaaacgtt gatcgttatt cacgatcaca
     2821 ctcgccccgc catatttttc acaaatatca ataatggctt gtggtaaatc cgcctctttt
     2881 gcctctacta catctgccat catcactcgg gcaaaatcga taaactcttg gcaaagttgt
     2941 ggttgagggc gatcggttaa acgggtaatc gcgtggttgt tttccaacac aggaattggc
     3001 tcaggcacat tttggcgtgg tctgcccata cgctgtgcca atttatttaa gaaattttca
     3061 cgattttgta aatccatttt cttatcctct gttattaaac cattcacgga aactttcgcc
     3121 ttcggcagtc ggtaaatcac gggctttcgt ccattcacct aacgccccaa aattcaccgg
     3181 tgctttgccg tttttaatca atttgcttgc cactttcgcc cccactttta cgccgacttt
     3241 ccaaagggtt gggtgtgagt tagcgaagtt aaagccaaaa atagacaagc gttctgccat
     3301 tggcgttaag ccggcatccg caattttctc acggtgtttt aagataagct gtgccagtgg
     3361 aattttcact ggacaaacac tgttacaagc ggtacaaagt gagcaagcat acggcaattc
     3421 tttaaactct tcgtagccac cgagcaatgg cgaaataact gaaccaatcg ggcccggata
     3481 gattgaacca tagccgtgac cgccaatttg gcgatacgcc gggcaagtgt tcaaacatgc
     3541 accacagcgg atacaacgca acacttcttg gaattcgctt tctaagattt tagaacgacc
     3601 gttatcgaca atgaccaagt ggaactcttc cggaccatcg gtttcgcctt caagtctagg
     3661 acctgtcagc caagtgttgt aagcggtgag ttttgcccct accgcactgc gagccagcat
     3721 ggtaattaat acatcaacct ctttaaaggt tggggcgata cgttccatac ccattactgc
     3781 aatatgcgtt ttcggcacag ttgttgccat acgcagatta ccttcgttgg tcactaaaca
     3841 aaccgagccg gtttccggca ccgcaaagtt acagcctgaa ataccgatgt ctgcctctaa
     3901 gaaatcttca cggatttttt ggcggacgaa tgcagtcatc tcttcaggtg tttccgaacc
     3961 ttgataacct agcacatcgt gcatttcttg gcggattctg tggcgatctt tgtggattgc
     4021 aggtacaaca atatgagaag gtttatcgcc cacaatttgc aataaatatt cgcccaaatc
     4081 ggtttcaacc actttaatgc cttctgcttc taaaacgtgg ttcatcccaa tctcttcggt
     4141 taccatcgac ttcgatttta cgattttctt cgcattttta gctttcgcca cgtttttgat
     4201 gtaatcagtg gcttcttcgg cggtttcggc aaagaaaacg tgtccgccgt tttgggtgac
     4261 tttttcgctt aattggtaga gataagcgtc taaattggct aaaacgtggt tacgaattgc
     4321 tttcgcatca tcacgccact cttcccagtt gcccagctca tccaccatac gctgacggtt
     4381 tgcaccgatt gtttcctgag ccttaactaa ggctttacgc acaatctcgt tattaacttg
     4441 ctgctcaaca cgttgtttaa acggcagggt gttagtttgt aaataagaca tcttattttc
     4501 ctccctgcat taacacttcg gcaatgtgca tcactttgat tggcttacct tcacggctta
     4561 aacgaccgcc aatgttcatc aagcagctta catctgcccc gattaaataa tccggctcat
     4621 cggtatctac atttgcgact ttctctttta ccatctcgcc ggaaatttct gccattttga
     4681 ccgagaatgt gccaccaaaa ccacagcaag tttcttggtt tttgattggt aataactcta
     4741 accctttaac gtgctgtaat aaagcaatag gctcatcttt aatccccagc ttacggaata
     4801 agctgcaaga cgggtgataa actgctttgc ccggtaaata agccccaaca tctttcacgc
     4861 ccagtttgtt cacaataaaa tcggttaaat catagaaacg atcagccact ttcttcgcac
     4921 gttctgccca ttctttttcg ccaaagcgtt caaaatgatc cggataggtt ttgatcgcat
     4981 atacacaaga acctgccggg gcgacaatcg ggtaatcatt agcttcaaag gtttcaacca
     5041 agtttttcat acctgctaaa gcttgcttag tgtaaccgct gttgatcgca ggttgtccac
     5101 agcaaccttg tttttctaag aaagtgattt tacaacctaa tttttccaat aaaagcacag
     5161 tattttttgc aacccctgct ttcattacat ctgcaatgca ggttacgtaa aaattaacgt
     5221 tcataaagaa ctcccacagt aaatataaat aagaattaaa ctcaaaaaat gccgttctaa
     5281 gacggcgaaa ggaagaggtg gatttgcaaa aaatttgcta aatctcaccg cttcctttct
     5341 aaatcaagta agctgattat taactcatta taggttatag aacataggta ctataaacaa
     5401 tgccgaaatc gcagcaataa cgccatacac gaacattggc acggctgttt tcttaataat
     5461 attgccttct tgtttctcaa tgccggtaac agaacacaca gccacgatgt tgttaataca
     5521 caccatattc cccattgcac caccaactga ttgcaatgca agaattaagg taacagataa
     5581 cccgacttgc tgtgccgttg catattgtac tgttccgaag gttaagttag ataccgtatt
     5641 tgaccctgag aagaacgcac caattgcacc taagaacgag gcaaataatg tccaagattg
     5701 accggtaaat tctgcgaagt ttttaccaat gatctgaacc attgagtcta caccaccaat
     5761 taacattaat tttaccatca caatggcacc taaaagtgct aagaatggtt ttttcacttg
     5821 tttaaatgaa gtgccaaaaa tctcttgagt atttttacct gataccgaga aaatcggtaa
     5881 gcaaattaat acggtcacaa caaatggaat caacgcagga acatacagta atttatagct
     5941 agttgctaca ttcgtaccta aaatattttt cagtgcaaaa attaagcctt ggctaatttc
     6001 aaagttagcg aaaccaagcg aactgacaat ccaagctgtt gcatcattta gcattgcttt
     6061 taatggaagt tgctgaatac gggtgattac caaaatgaag atcagtaacc ctgttggtaa
     6121 taatgctttt gccacttcac caaaggtcgc tttatcgccg tcaaggtggt tctcgctttt
     6181 cgctaaacca atacccatat ttgctaccca gactgacacc aataaaccaa tcgccccggc
     6241 aactaatgat gggaattcgt agttaaattg agcaataatg aagtaaggaa tgacacaggc
     6301 aaaaatgctg atataaataa acacgatatt ttgacgaatt tctttccagc tcacaattaa
     6361 acgcaatgcc ataactggaa taataaacgc agcaatagag tggatcagtg cagtaataga
     6421 accaatttca agcacttgtg catcggttag taaaccatct tttaataatg gtcccatacc
     6481 aaaccaagtt ggtgtgccaa ccgcaccaaa tgacaccggc acagagttca ttactaaggc
     6541 taacattgcc acttgtaacg gtttaaagcc taaaccaacc agaattggag ctgcaattgc
     6601 cgccggcgta ccgaaacctg atgcgccttc aatcataaat gcaaatgccc aaccgataat
     6661 catcaactgt gccaccggat tcgggttaat tgtccctaac catttacgca tagtgttggt
     6721 tgcacctgaa acttcagaga aacggttaaa taaaatcgcc ccgaaaataa cggtaattgg
     6781 ggttaataca tcacctaaac cggcaataat attggcaaag attaacgtgg tatcattacc
     6841 aaaataggta agctgaagca caaaaattaa taacgccgta agtggtaaag ccacataaga
     6901 cggtaacgcg ttacgtttta ccattaaata aatgagcaaa acaattggaa atacgctcag
     6961 gaatagagcc atactgcacc tctttatatg attataaaat gacctaactg gtcggatagg
     7021 tgcgaattgt actcttaaaa aaaactaatg taactaggtt gttacaattt tgtgatctag
     7081 ctcgcatttt taatgattgt atggttaaaa aagagccata tttattttca tttaatactt
     7141 aatagttagc cctatttaag acatttttac cgaatcgagc aatatgcaat taattaacaa
     7201 aaaaaggccg cttgtagatc acagatcaac aagcggtctt ttttaggcaa aaatttgcaa
     7261 attaaacatt gaaacggaag tgcattacat cgccgtcttg aacaatataa tctttacctt
     7321 ccaaacgcca tttacccgct tcttttgcac cggcttcacc tttattgtct atgaagtctt
     7381 ggtaagcaat tacttccgca cgaataaagc ctttctcaaa gtcagtgtga atcacggctg
     7441 ctgcttttgg agcggttgca cctaccgaaa ccgtccacgc acgcacttct tttacccctg
     7501 cggtaaagta ggtttgtaag ttaagtaaac ggtagcctgc acgaataaca cggtttaagc
     7561 ccggctcttc aatgcctaaa tcttgtagaa attccacttt ttcttcgtca tcaagctcgg
     7621 caatttccga ttcaatcgct gcacacaccg gtacaaccac cgccccttct ttttcggcaa
     7681 tttcacgcac acggtcaaga taagggttat tttcaaaacc gtcttcgttc acgttggcaa
     7741 tatacattgt cggttttaaa gttaagaagt tgtagccctt aatagcgtgc agttcatcct
     7801 tatctaaatc taccgaacga atcataccgg cattttcaag cacaggtagg attttttcca
     7861 taatcgataa ttcaaatttt gcgtctttat cgccaccttt cgcacgtttt tgtaaacgct
     7921 gaatggcacg ctcgcaactg tctaaatcgg caagtgccaa ttcggtattg atgatttcaa
     7981 tatcatcagc tgggtcaatt ttccccgcaa cgtgaacgat gtcatcattt tcaaaacaac
     8041 gcacaacgtg gccaatcgca tcagtttcac gaatattggc taagaattta ttacctaaac
     8101 cttcgccttt gcttgcacct gccactaagc cggcaatatc cacaaattcc attgtagttg
     8161 gtaacactcg ctcaggtttc acaatttccg ccaacgcatc taagcgaggg tcaggcattg
     8221 gtaccacacc ggtatttggc tcaatcgtac agaacggata gtttgctgcc tcaatacctg
     8281 ctttagttaa tgcattgaaa agggtcgatt taccgacatt tggtaaaccc acaataccac
     8341 atttaaatcc cattttttgt tccttagaat tcccctctcc ccttgcggga gagggataaa
     8401 tttgagcttc gttcagaagc gaaaatgagg gagagggcga aattgcaata atttcctcaa
     8461 atatgaccgc tcccctctct ctgaaaaata cttctgaacg aagtattttt cggtctctct
     8521 cccacaaggg agagagtttt aatttgcttt aaaactattt aaacggttag ttgccttaac
     8581 cattccgtct ttaaataaaa cttctacgca acgtgcggct tcatcaaccg cactattcag
     8641 taatgtctga tcgcttggtg aaggcttgcc cagcacataa ttcgccacta agtctttatg
     8701 ccccgggtgt ccaataccaa ttcgcacacg gtaaaactct ttattattgc caagggcggc
     8761 aataatatct ttcaagccgt tatgaccacc gtgtccgccg ccttttttca atttcaccga
     8821 accgggtggt aaatccaatt catcgtgagc gaccaaaatt tcttccggtt taatccgata
     8881 aaaattggct aacgctccca ctgctttacc acttaaattc ataaaagtag tcggcactaa
     8941 taaacggact tcgccttgag cggtattaat ttttgctact ttgccaaaat atttcggctc
     9001 ttcttttagg ctaacgttat atattcgggc aagttcgtta atcagccact cgcctgcatt
     9061 atggcgggtt tcttcatatt ttacccccgg gttcgccagc ccgacaatta atttaatttc
     9121 tgacacaatc ttgtctcaat tcatttaatt taaaaggcag tattttagag aaaaaccgct
     9181 attttctcaa ggcaaccatt aatcatattg tctatacgga tttggtacgc attgatactt
     9241 cgctaacatc gattcagtga aatcggtgcg tgaagcagcg ttatcattgt ttaaactaac
     9301 cacaccaatt cgcttgcctc tgcaatcccc tttagcaata aaggcaagat tataacccgc
     9361 ctcacgggta taacctgtct tactcactaa tgcctgatag gattgggaac gaaccatact
     9421 gttggtgttt ttcatagaga aataacgacc acctgcataa acactcacta atggcgaatt
     9481 agacaattgc tgaatttcag gcttacttgc cgcatagcct gccaatttag caagatcaac
     9541 caccgttgaa acattatccg aagataagcc tgagctatca ccaaagtagg tttgagtcat
     9601 accgatttca cgggctttgc tgttcattcg agccaaaaat tgggctttat tcaaatgtgt
     9661 tgcgtgggca agggcgtgag cggcatagtt atcggagcga actagcatcg cttttaacaa
     9721 ttcactacag gcaatgttaa gatttttcgg caatgggctg gtcgtgcctt ttaagaaatc
     9781 agcatctgac ggcaaaattt gggtttggca actcgcccca tagcggtttt cttctaagaa
     9841 aacgactgct gtcattaatt ttgtgaccga agcaatcggg cgaactaaat tgaaattctt
     9901 accttccaaa aatttctttt ggtttaagtc aaacaccaca taagcggaat tttcggttgg
     9961 aatataagca gcggttgctt taatccgtgg tgtgcttttc ctttttgccg gctgggagga
    10021 acaggcgctt aacaccagag ccgcaatcat aacaggaata aattttttca tcataataga
    10081 ttcatcgata ataaagtcaa agctggttat tctaccctgt ttccaaaatt gtgccaaagc
    10141 aagcggtcga tttttacaaa atttttgcaa atctagccga ttttgcaaaa tttccgccaa
    10201 atttaaccgc ttgccctctc gcctcaaatc agcgaaaata acgcttaatt ttttggcgga
    10261 ctttccgccc tttttatttt aattggttga gaaaaatgtc atacccacaa gaagttaata
    10321 aacgtcgcac ctttgcgatt atttcccacc ccgatgcggg taaaaccacc attaccgaaa
    10381 aagtattgct ctacggaaat gcaatccaaa ctgccggctc ggtaaaaggc aaaggctcgg
    10441 cacaacacgc taaatccgac tggatggaga tggaaaaaca acgtggaatt tcgattacca
    10501 cctccgtgat gcagttcccg tacaatgatt gcttggtaaa cttactcgat acaccggggc
    10561 acgaagactt ctcggaagat acttatcgta ctttgactgc ggtagatagc tgcttaatgg
    10621 ttatcgactc ggcaaaaggg gttgaggaac gcaccattaa attaatggaa gtgacccgct
    10681 tgcgtgatac gccaatccta acctttatga acaagctaga ccgtgatatt cgtgacccga
    10741 tggagctgtt agatgaagtg gaaagcgtgc tgaaaatcaa atgtgcgccg atcacttggc
    10801 cgattggttg cggaaaattg tttaaagggg tatatcacct ttacaaggac gaaacctact
    10861 tataccaaac cggacaaggt cataccattc aagaagtacg agtggttaaa ggcttaaata
    10921 atccggaact ggatcaagcc gttggcgatg atttagccca gcagttgcgt gatgaattag
    10981 agctggtaca aggcgcttcg cacgaattcg atcacaccga atttatcaaa ggcgagctga
    11041 caccggtttt ctttggcact gcactgggta acttcggggt caatcatttc cttgacggtt
    11101 taaccgaatg ggcaccggca ccacaagccc gtcaatcgga cgaacgtgaa gtttcaccaa
    11161 gcgaggaaaa attcagcggt ttcgtattta aaattcaagc gaatatggat ccgaaacacc
    11221 gagatcgagt tgcttttatg cgtgtcgttt caggtaaata tgaaaaagga atgaagctta
    11281 aacacgtccg tatcggcaaa gatgtggtga tctccgatgc cttaaccttt atggcgggcg
    11341 accgttcaca agccgaagaa gcctacgccg gcgatattat cggcttgcac aatcacggca
    11401 cgatccaaat tggtgatact ttcacccaag gcgaaaattt caaattcacc ggtattccga
    11461 acttcgcccc tgagctgttc cgccgaatcc gcctgcgtga tccgctcaaa cagaaacaac
    11521 tgctcaaagg cttggtacaa ctttccgaag agggtgcggt gcaagtattc cgtccgttaa
    11581 tcaacaacga tttgatcgtg ggagcggttg gggtgctaca gtttgatgtg gtggtttcac
    11641 ggctgaaaac cgaatacggt gtagaggcaa tttatgaaac cgtaaacgtt gccacagccc
    11701 gttgggtcga aggcaaagat gccaaaaaat ttgaagagtt caaacgcaaa aacgaggcaa
    11761 accttgccct agacggcgga gacaacctca cctacattgc tccgacaatg gtgaacttaa
    11821 acctcgccca agaacgttac cctgacgtac agttctttaa aacgagagag cactaaaacc
    11881 aataggggcg aaaaatcttt cgcccctaca aataaagcac aagcggtcaa ttttttttcc
    11941 gaattttaca atactaggca ttctgttcct caaatagcct aaatattccc ctatcaacaa
    12001 tccttatctt gagattattc tgcctattaa ttaaccactc aaaaataccc tcgcagaaaa
    12061 ctctactttt tttatggaaa tatgctaaag ttcgccaata ttcatagccc tgattttttt
    12121 atggagaaaa taaaatgaaa aaatggttga ttccattaac tgtcgcagcg ttctgtgttt
    12181 ctgcttgcga tgataagaac agcactcaag caaccgcaga aaaaccgcaa gtagagaata
    12241 ctcaatcgca aaaaaatgcg gaaatattac cgcttgcaac cgtggttgcc ccatgggaaa
    12301 tcaatagcct tgacccgaat caatcaggcg taattttcca gcgtatgaac ttagcggaaa
    12361 cattggtcga agccgatctt aacgggaatt taattccggc attggccact gactggcgca
    12421 gtaacgaaga gggaacaatc tggcgtttta ccttgcgtga aggggtaaaa ttccataacg
    12481 atcaagtact taccgcacaa gcagtggtaa atagtttgaa tatcgcaaaa agcaaaccgg
    12541 gtgtgttagc aaaagccttt atcaaggaaa ttcgagcggt agataacaaa gtggaaattg
    12601 agctaactaa agcttttgtg ccgtttcctc cctttttaac acactacacc gccttaattt
    12661 tggcacctga aagctataat gaaaaaggcg aagtgaccca aattatcggt acaggtgcgt
    12721 ttaaacccac aaaaattgaa gctccgcaga aattggaagc ggtgcaattt gagggctatt
    12781 ggggcaaaaa accgcaagtt cagcaagcca attatttagc cagcagccgt agcgaaaccc
    12841 gtatgttaat ggcacaaagc gagccgtcat cactggtatt taatttagat accgccagtg
    12901 tagcaaggct gaaagccgat cctaacctgc aattagtcag cggatctatt gcccgtacta
    12961 ttcagttgaa aatggacgtg gcaaagccgt tttttaacga cttagcgatt cgccaagcct
    13021 tatcgcaagc gattaatcgc aaggcgattg ctgagcaggt cttaaaaatt gagaacggag
    13081 ctgccgatca aattttaccg aaagcctttg ccgactggcg tgttgatacc gcagaacaag
    13141 tggtcgatat tgcaaaaatt aaacaaaatc tgaccgcttc cggctatacc tttgatgaac
    13201 aaggtcgctt aaccaaagac ggcaaaccgt ttagctttac catgcgcacc ttctcagatc
    13261 gtcctgaatt accggtgatt gctactatct tacaagctca atggaaacaa attggcgtgg
    13321 aagtgaatgt ttcggtgggt aatttcagcg aaattccggc ggggcatcaa gacggcagtt
    13381 tagaaatggc tctttatgcc ttaaattatg gcaaaacctt agatccatat ggcgtaattg
    13441 cctccgactt cgccaatggt ggcagtgatt ggggggtaat gaattggcaa aatactgagg
    13501 tagacaatgc agtcaaatcg ctcgaaaccg agcgtgaccc gcaaaaagcg aaagcattaa
    13561 agcaaacggt tagcaaaatt atttacaatg atttgccaat cattccggtg gtttattacc
    13621 aacaaaatgt ggtggcacat aaagaagtaa aaaatgtgac gctagatcca tttgaaagac
    13681 gcttcttttt ggagttatta agtaagtagt ttaattcaaa atactcctcg ctttagaaaa
    13741 gggaaggtag gatttttcag tagtaatttg cgctcttagg tgaataaata gtgacagaat
    13801 tatcgttttt tctcattttt cacctatttt ctccgcttat ttcttctgcc aaatcttccc
    13861 ttcccctctt tgctaaagag ggggatcaga acgcataaaa tcatcttttt aataaatgaa
    13921 ataatacaat gcacaaaatt ctcttccgcc gtcttatcca aattctactg gtgatttggt
    13981 cggttggcac actcacattt atcctcaccc gccaactttc gggtgatatg gcataccgca
    14041 ttgctgccag ccgctatggt tatgatcaag cagatagtgc tgctgctgag gctgtgcgtg
    14101 ccgagctggg attagaccaa ccttggtggc aaagctactt cagctggcta agtgacttgg
    14161 ctcaatttaa tttaggcaaa tcattggtga ccggtgatcc ggtctggtct gaaattcagc
    14221 accaattcgg gcatacctta agccttgcga ttgtggcact tatcttagca atccttatcg
    14281 ggcctccgct cggtttgtta gccgcccgta agccaaacgg cttttttgac cgcttgacac
    14341 tggtattcag taccctgttt cgctccgtgc cggcatttat tattgcgatt ggcttgatta
    14401 cgctgttttc cgctcaacta aaatggctac ctgccggcgg atacggctgt tggcaacatt
    14461 ttatcttgcc tgccttaacg cttgcgattg gcttgagtac ggtttcggta cgagtcaccc
    14521 gttctgcaat ggttcaggta aaaggctcag aattttacca attttcacaa ttaaaaggct
    14581 taagccgttg gcataccttt ttacgtcacg gtttgccgaa tattgccgtg ccggtcattg
    14641 cctaccacgc agtacaatta gtttatttaa ttgaaggcgt tgtggtggtc gaaagcctat
    14701 ttgcttggcc cggcagcgga cacgcactgg ttcacgccat cattgcccgt gatgtgccga
    14761 tgattcaagg tacaacgctc gtgatgggcg gattgtttgt tgtattaaac accattgccg
    14821 atctattaag tgcttggatt gacccacgaa ttaagcgata ggacatcaag atgaaaattt
    14881 caatttcaca atatttaggc ggcactattc tccttgcaat gttgggattt gcctttttac
    14941 agccttattt ttacccgatg gatattggct ttcaagattt aaccaatatc ctttccaatc
    15001 caaatgaaat cgcttggttc ggcaccgatc atctcgggcg ggatatgctt gcccgtcttg
    15061 cctctgctat tcgtctttcg tttggcttat cactgtttag cgtgatttgt gccttaattg
    15121 caggcttaat taccggcatt atggcaggct ttttcggtgg ctggattgac cgcattttca
    15181 gctttatttg tgatctagtg atggcactcc ccggcttact tttagtattg ctctttgccg
    15241 ccctttctcc cggctcattt tggacgctct atttagggat ttcattggta atgtgggtgg
    15301 aatttttccg tgtgatccgc tctatcagtt caacaatggc aaacagctcg gaaattcaag
    15361 cctcacgcct aatgggaatg ggctggtttt atagcttcaa acgccactta ttgccccgtt
    15421 tattaccgat gattgccact ttatcggcat tctcgctcgg caatgcgatt ttagctctcg
    15481 ccacacttgg ctttgtcagc gtagggcttc gcccgcctac agcagaatta ggcttgatga
    15541 tgaccgaact attcccttac tactacgaag caccgctgat atttttgcaa ccagtggttg
    15601 ccgttttctt aatggtactc agcttacaat tattgtctag aaaaattaaa taatgcgtag
    15661 gggcaggtcc tgtgcctgcc caaaattctt attatattta tgggcgggca cagggcccgc
    15721 ccctacagta ataaaatgaa gatgaaatta ctcgatatcc aaaacctatc tgttgaaacc
    15781 gaaaacagat tacagctggt cgaaccgatt agcctacaat taaaggcagg aaaaaatatt
    15841 actatcttag gcgaaaccgg ctcaggcaaa agcctgctga ttcaagcgat tatgggggcg
    15901 ttacccgacg gcttaatcgc aagcggtgaa atttcggtaa aaaattgcaa aatttccacc
    15961 gaatccgacc gcttgcaaac attgtggggt aaaaccttgg taatgctgcc acaagagcct
    16021 cgccgctcgc ttaatccgat tatggcaatc ggcaagcagt tgtgggaaag ttttcactat
    16081 gtggcaggca aaccatcatc tcaagccaaa acggaaagca aagcctattt agccaaactt
    16141 gggctgggcg aatcggttga aaactaccca caccagcttt ccggtggaat ggctcaacgt
    16201 gcctcttttg cgattgcaac tgccgccggt ggcaaaattt tacttgccga tgagccgacc
    16261 aaagggcttg acccaatcag taaaggcaaa gtgattgaac tgttaaaatc cgcctaccaa
    16321 aacggtggag gcttgctcac cattactcac gatattgaag ttgccgagca actcggtggc
    16381 actattttag tgatgaaaaa aggcgaactg ttggagcaag gtgaggcaaa agcgttgtta
    16441 aataacccac aacaccctta caccaaagca cttatcagtg ccgatccaaa acattggcaa
    16501 ccattgcaag atctgcaaaa tttaagccaa aaacaaccgc ttgtttcggt taaaaatttg
    16561 agtattgccc gtggtaagaa aacgctgttt agcaaccttt cctttgactt acacaaaggc
    16621 gaagtgcttg ggattgtcgg gcatagtggg attggcaaaa gctcgcttgc cgatgtactt
    16681 tgtggtttgc ttaagccgaa atcgggcgag gttgtgtggc acagccacag ccataaaaaa
    16741 caccaagtgt tgaagcttta ccaagaccca ccggaagcct ttgctccgaa tgtcagtcta
    16801 aaaaccttgc ttgatgatgt gattaacaag caccaattag atcgactgcc gattccaagt
    16861 ttactgcaac aactttcgct caacccagaa attttgcaac gcaatgcgga aaatgtctcc
    16921 ggcggcgagc tacaacgagt agcaattttg cgagccttgc tcttagaacc ggtactactg
    16981 tttgccgatg aagtcacttc tcggcttgac ccaatcacac aacaagaaac gatagaattg
    17041 ctgatcaacc agtgccgagc gagaaattgc agtctgattt tggtcagtca cgatccttat
    17101 ctaattgaga aatcgtgcga taaagtgatt aatttggaag agtatgtggg ctaacaagcg
    17161 gttcaatttc tgagattttt tgcggctcta aaaacaaaaa tgcgaagaca tcagttcttc
    17221 gcatttttct tacttattcc ttattgccca gccagcttct gccgtacaat ttcaaacaga
    17281 cagaccccgg ttgcaactga gacattcaaa gaagatactg agcccgccat tggaatgctg
    17341 ataagctggt cgcaatgctc acgggtaagg cgacgcattc cgtcgccttc tgcccccata
    17401 actaacgcaa tcgagccggt taattttgct tcataaatgc ccgattccgc ctcacctgcc
    17461 gtgccgacaa tccagacatt atgccgttct tgtaaatctc gcattgtgcg agccagattg
    17521 gtaactcgaa tcagtggcat cacttccgct gccccacagg cgactttgcg agcggttggt
    17581 gtaagctgag cagatttatc tttcggcaca ataaccgcat caacccctgc cgcatcagcg
    17641 gtacgcaaac aagcccctag gttgtgaggg tcggtcacgc catctaaaat caacaagagt
    17701 ggatttttct tttggcttaa gatcgcctct aaatcgtgtt cattcagctc tttttgtggc
    17761 acgactctgg caataatccc ttgatgcact tcaccttgcg atttgttatc taaggtttgg
    17821 cggttcactt gttgaattgc cacccctaaa cgttgtaatt catttaatag tggcattaag
    17881 cgtttatctt cacgcccttt tagcaccagc acttcaatta aacgctcagg tgcattatcc
    17941 aaaaaggctt tcactgcgtg aatgccataa atttgttcgc tcatatttta tccttaataa
    18001 ttgggcgaac ccatcagctc gcccatataa atacctaact aaagcaacat cttcacaatt
    18061 ttatccaact taaccctgcc gaattttttc agcaatttac gcacattttc agggtaatcg
    18121 gttaaaactt ccaaatcact gtagtgagtt aaaatctgcg tatgttggca aatttggttt
    18181 aactccgttt ctgctttttc acttaattga tgttgaggat cggaaattaa aattgcattt
    18241 tcggcatcta accgccacgc tcttgggtta aggttattgc cggtcagcaa aatatagcgg
    18301 ttatccaccc aaaccccttt taagtggtag ctgttttcgc catctttcca taagcgaatg
    18361 gttaactgtt tattttgtac ataaccgtcc aaccgttttg caaaggctcg taagttcttt
    18421 tcatacaaat aaggcagagc cgatgccatc gtgaattttt cgtccggctt ggtgtagaaa
    18481 tcattagcgg ttttatcccc cacaataatt tcaaccgatt tgccgtttgc caatagccat
    18541 tcaatacggg ttctcagaga atgcggaaaa ttaaaatacg gagtgcaaat cgttaatttc
    18601 tgctcagttt gataaaacaa cgcttcaatc actttattta actgattctt acgtcgccct
    18661 aagcctacta aaggagaaac cgctaagcga ccattttcag caacaccgtt aaataaatac
    18721 gcttcccgac ttaattgttt gcggaaatgc ttaatttgcg cacgaatttc agccgttttc
    18781 gggcgatttt ccgtatctaa acgttttacc gcttcattgg ttaaaagatg ctgttgaata
    18841 aacgtcaccc agctatcggc aagaacttta ttttcaatga cgtggtaacg gtcataacgg
    18901 taacgttcaa attgatgcaa atacacatta ttaatgcttg ctccgctata caaaatcgta
    18961 tcgtcaaata cgaagccttt caaatgcaat acaccaaaca cttctcgtcc gttaatcgac
    19021 acaccgtaaa aggcaatctc ttgccctgcc ggtaaacctt gttcgtattt aacttttgca
    19081 taccagtcgg cgttagataa attctctgcc tcaccaatac gcccacgttg ggctcggtgc
    19141 caatcaacca aaatgttaat ttccagttca ggattatttc gcttcgcttg atataaagca
    19201 gctaatactt cctgcccggc ttcatctttt tcaaagtaaa gagcggttat ataaatacgc
    19261 ttttttgccg tttgaatcag ctgtaaaatt tgttgtttaa acgctttgct cgaggcgaga
    19321 aactgaacct gctctgccac ttgtggtaag tatggcaggc ttttcaatag gtattcagct
    19381 ttacgttgtt tgttcaaaat taacatcatt gattccaaaa ttttcaatcg ttgcctgcta
    19441 ttttacactg aaatcgccaa taaagcgatt ttcgcttaac ggcattgata gctggtcaaa
    19501 cgattaggat cttctccggt ttgataaatt gcgactttcg gataagcaca aagcgggatc
    19561 tgcttggctt tattagggta aagctcaccg gctttggcgg caatactttc cggctccagt
    19621 cctttctcaa cccaattttc aagggcagtg agcatatcaa aatcattcac cgttgcccct
    19681 cgcccacaat ggttcattgc cgacacgaaa aacaattttg caaaatgttg cggattttcg
    19741 accgcttgtt gtaggctttg ataccaattt ttgagatcaa ctgccgaaaa aatcgggtct
    19801 gaaacgcctt gataaatcag cattttgcca ccgttctgct taaaagcgga taaatcaact
    19861 tgatctgtat cgtgaatgct ccctaccgct ttcacttgtt cgctggcttg atccaaatct
    19921 acgctgagtg gatcacggct cggcactctg ggggctaaat agtaatgggt caaacttttc
    19981 aatcccattt taaaactaat gctgttgggc tgagcggttt ggctatcacc taatttccaa
    20041 tcccgccagc ctttagcgtt aatgccggaa tcatacgccc aagggctata aatcagctcg
    20101 cctcgactgt ttttcgcccc ctcgaaaatc gttttgatta aagcgacttt tttctgcccc
    20161 agttgctttg ccaccatttc cggctggaaa tcacactgtt cccacgcatt aattaaaccg
    20221 tctgctaagc cgtctaactt atcgcaacgt gttaatacgc cattggcaat cacatctaaa
    20281 tcttgttgag tgagggcttg ggataaaatt ttgtcgccat caccattctt cggagcaact
    20341 gccaacaagg ctcgattatc ccatacttcc gccaagactg aacgggaaac ccgaaagccg
    20401 gggctgcctg caatcacacc gtcaaattcc tgtggataac gcattgccgc catcatcgcc
    20461 tcacgaccac cattcgagca gcccataatg taattgtgct tgctcggttt tcggtaaaat
    20521 tcggcaatca actgcttggc aaccgtggtc actttgccga tagaggcata ggcataattt
    20581 gagcgagctt cgggatctgc ggtaaacact aaatctcgag tggcgtgacc actgtcgtta
    20641 ctcactactg catagcctct gtttaagcca tttgcaccac tcgccccgtg cggatagagc
    20701 ttaccaatgg cagggaaaat caccccacct acaccgcccg ccccctgaaa taagaatttt
    20761 tcattccatt gttcgggtaa acgtagctca aattgaatac cgtaaggaac attatccacc
    20821 tccacccgtt ttccaatttc accttgtact aaacagtaag ccggcaactc agcagatttc
    20881 tcaacccatt cagctttata aatagccgtt ttttccggcg agttagattt taacgcgaca
    20941 cattgttcag ccatagaatt agccaaagca gtgtgagaaa aaacggagag aaaaataaaa
    21001 agaaaagcga ttattttcat catttacatc aatacaagcg gttatttttc cgaaaatttt
    21061 tgcaaatttt aaactgagtg tctatctatc gctggaaaag ctaaggcaga gtttcctctg
    21121 ccttagaaag atgaattgaa ttacatttta agcaaacaag cgtttttcgc cttcgccggc
    21181 tacgttttcc acacaacgtc cgcaaacttc ttcgtggcca gagtgcgagc cgatgtcggt
    21241 agcataatgc cagcaacgag ggcatttctc accatctgca ttttccactt ttactgcgag
    21301 tccggctaat tcgccttccg caatatctgc ttccgccagt ggtttaacaa tcgcttgtga
    21361 ggtaatcagc acaaagcgta attcattgcc aagttgttct aataacgggc gaatttcatc
    21421 attggcgaat accgtcacct tggcttctaa gccagcacca attactttgt ctttacgggc
    21481 ttgttccagc acacggttga cttccgcacg cacttttaag atttgttgcc agtaagcatc
    21541 atctaattta tcttgctcgg ttaaaccgaa taatccggtg tagaattctt cggtaaagac
    21601 aaattcactt ctgccttcca cctgcggtaa gtagccccaa atttcatcgg ctgtgaagga
    21661 taaaatcggt gcaatccaac gtactaacgc ttctgcaata tgccataacg ctgtttggca
    21721 gctacggcgg gcaaggctgt cggctttggt ggtgtattgg cggtctttga taatatctaa
    21781 gtagaaagac cccatttcaa tcgagcagaa acgcattaaa cgctgtacta ccgtgtggaa
    21841 ttggtagtta tcataggctt ctttgatgtc gttttgagcc tctaaagcac aagccaccgc
    21901 ccagcggtct aaggcgatca tctcttgcgg ttgaactaaa tcactttttg gatcgaaacc
    21961 gttcaagttc gccaataaga aacgggctgt gttacgaata cggcggtatg attccccggc
    22021 actgtttaaa atattgtgcg aaacggtaat ctcaccggta tagtcggtgg aggcaaccca
    22081 taagcgtaaa atatctgcac cgtttttgtt ccacacttca cttggcacaa ttacgttgcc
    22141 aagcgatttg gacatttttc tgcctttctc atctacggta aaaccgtgag ttagcacttg
    22201 tttgtatggt gctttgccgt tagtagcagt ggaaagcatt aaagatgaca taaaccaacc
    22261 acgatgttgg tctgaacctt ctaaatacat atccgctgaa ttgccgttaa attcagggcg
    22321 ttgttctaca acagaggcat aggtcgatcc tgaatcaaac cacacgtcta aggtatccgg
    22381 cactttgcgg taatctttgg cttcttcacc gagcaattcc gcaggatcta aatcccacca
    22441 cgcttgaatg cctttttctt ctacacgttt cgccacttct tcaattaatt ctaaagtgcg
    22501 aggatgtaat tcttcggttt cattatgcac aaacatcgcc attggcacgc cccaagtacg
    22561 ttggcgtgaa atacaccagt ctgggcggtt tgccaccatc gtatcaatac gagcttcacc
    22621 ccagcttggg atccaacgca ccgatttgat ttctcctagt gcttgctggc gtaagccttg
    22681 ggtttccatt ccgataaacc attgtggggt agcacggaag ataatcgggg ttttgtgtct
    22741 ccaacagtgc ggataactat gcttgatgcg ggataatttt aataatgctc ccacttcttg
    22801 taatttttcg actactttat cgttggattc aaacacacct aagccggcaa agaacggggt
    22861 gtctgctttg aatttaccgt cattggaaat tagccctgcc atttcaatgt tatatttgcg
    22921 agcgaccacg aagtcgtccg caccgtgatc cggtgcagta tgcactaacc ctgtaccacc
    22981 gtctgtggta acgtggtcgc caaaaataaa tggcacgctg taatcataga atgggtggtt
    23041 aaagcgtaat aattctaagt tttcgccttt ggtggttgct aaaatctcag cagattcaac
    23101 gcctgctgct tttgcaacat cttcgactaa atcgaccgct agtaacacac gctcatcacc
    23161 taattggact aaggcatagt ctaattcagg gttgatcgca atcgcacggt tagacggaat
    23221 agtccaaggg gtagttgtcc aaataaccgc agataatgcc cctctacctt taccctgtgc
    23281 gttgaatttt tcttctaccg catttacgtc cacagcatta aaacgcacat aaatagaggg
    23341 agaaacttta tcttcatatt ccacttcagc ttccgctaac gaagatccac aatctaagca
    23401 ccaatgtacc ggttttgaac ctttgtataa atgcccgttc gcaatcactt tgccgagcgt
    23461 gcgaataata ttcgcctcag tgttaaaatt cattgttagg tatgggttat cccaatcacc
    23521 taaaataccc atacggataa aatcggcttt ctgaccttct acttgttctt tcgcatagtc
    23581 acggcacgct tggcgaaact cggcagccga aattttctca tttggtttac caacaatccc
    23641 ttctactttc agctcaatcg gcaaaccgtg acagtcccag cccggcacat aaggggtatc
    23701 gaaacctaat gcggttttcg atttcaaaat catatctttt aaaattttat tcactgcgtg
    23761 acctaagtga atattaccgt tagcatacgg aggaccatca tgcaaaataa aggttttctt
    23821 gcctttcgag gcagtacgaa ttttttggta taaatttttg tcataccaat tttttaacat
    23881 atcgggttca cgtttcgcga gatccccacg cataggaaag cctgtttcag gcaagtttaa
    23941 ggtgtttttg tagtcaatag tcattttttt atttctcgtt aaaattatct gttttatgcc
    24001 tagcacggca tagccgtact aggttaagca tagctcagcc cctctggggc tggaaactag
    24061 aaaggctcac cttgcaaatt ccttaaaaaa ttcaaccgct tgcaagcgat ctttctcaat
    24121 ctgggctttt agtgcttcaa agtcgggaaa ttttatttca ttgcgtaatt tttttaaaaa
    24181 gatcacttca atcgcctcac cgtaaataga ggcattgaaa tcaaaaatat gcacttctaa
    24241 taacggctta gtaccgttaa tggtcgggcg gttgccgaca ttggcgattc cgttaaaact
    24301 gccctgtttg gtcttcactt ttaccgcaaa taccccttga atcggcgtaa ccaaacgatt
    24361 gagcataata tttgcggtag gaaaaccaat ggttctgccg agtttattac cgtgtgcaac
    24421 gcgtcccaga atcgaataag gcttgccgag taatttctct gccaaatcta actcattgtt
    24481 ttgtaaggct tctcgcacta atgagctgct aattctttct tcgtttaagc tatgggtatg
    24541 gctttcttcc accgcaaagc gatattttaa gccggcattg cgtaaagttt caaaactgcc
    24601 cgcccgtttt gccccaaagc gaaaatcatc gcctacactt aaatagcgta ctttcagtct
    24661 tttagcgagc aaatcgctga tgaaatcgtc tgccgaaatt tgagcaaatt tttcactaaa
    24721 acgcacgcat agcacaaaat ccacaccggc ttcttccaaa tattttagct tatctcgcag
    24781 gcgcattaag cgggcgggcg ggttacaagc ggttgaattt ccactttttt ttgcaaaaaa
    24841 ctctcgaggt tgtggctcaa ataacatcac aactgagggc aatcctaaat ctaaagctct
    24901 atcacaaaga cgagccaaaa tattttgatg cccaagatgc acaccatcga aattcccgat
    24961 agagagaaca cagccttgat ttaaagcgat gtgtttgtct aagttgtgga aacctcgaat
    25021 aagttgcata aaagataaac cattttataa gtgaaaattg ctgtgattat aacgagaaat
    25081 ctacaaaaaa ggaaaaaaat tcaatctg
//
LOCUS       DANLUA010000012        22628 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLUA010000012 DANLUA010000000
VERSION     DANLUA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            69..1175
                     /gene="mltA"
                     /locus_tag="PXB44_000418"
     CDS             69..1175
                     /gene="mltA"
                     /locus_tag="PXB44_000418"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL5280933.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            1188..1637
                     /locus_tag="PXB44_000419"
     CDS             1188..1637
                     /locus_tag="PXB44_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280934.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(1671..3188)
                     /gene="purF"
                     /locus_tag="PXB44_000420"
     CDS             complement(1671..3188)
                     /gene="purF"
                     /locus_tag="PXB44_000420"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5280935.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(3203..3706)
                     /locus_tag="PXB44_000421"
     CDS             complement(3203..3706)
                     /locus_tag="PXB44_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL5280936.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(3852..4313)
                     /gene="rnhA"
                     /locus_tag="PXB44_000422"
     CDS             complement(3852..4313)
                     /gene="rnhA"
                     /locus_tag="PXB44_000422"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL5280937.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(4406..5737)
                     /gene="argG"
                     /locus_tag="PXB44_000423"
     CDS             complement(4406..5737)
                     /gene="argG"
                     /locus_tag="PXB44_000423"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL5280938.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            5921..6961
                     /locus_tag="PXB44_000424"
     CDS             5921..6961
                     /locus_tag="PXB44_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5280939.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(7026..7784)
                     /locus_tag="PXB44_000425"
     CDS             complement(7026..7784)
                     /locus_tag="PXB44_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5280940.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            7911..8225
                     /gene="yhbY"
                     /locus_tag="PXB44_000426"
     CDS             7911..8225
                     /gene="yhbY"
                     /locus_tag="PXB44_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL5280941.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            8286..9008
                     /locus_tag="PXB44_000427"
     CDS             8286..9008
                     /locus_tag="PXB44_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL5280942.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            9077..9238
                     /locus_tag="PXB44_000428"
     CDS             9077..9238
                     /locus_tag="PXB44_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280943.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            9246..9608
                     /locus_tag="PXB44_000429"
     CDS             9246..9608
                     /locus_tag="PXB44_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280944.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            9621..10538
                     /gene="nagK"
                     /locus_tag="PXB44_000430"
     CDS             9621..10538
                     /gene="nagK"
                     /locus_tag="PXB44_000430"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL5280945.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNNLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(10609..12558)
                     /locus_tag="PXB44_000431"
     CDS             complement(10609..12558)
                     /locus_tag="PXB44_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL5280946.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(12591..13379)
                     /locus_tag="PXB44_000432"
     CDS             complement(12591..13379)
                     /locus_tag="PXB44_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL5280947.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            13504..13734
                     /gene="xseB"
                     /locus_tag="PXB44_000433"
     CDS             13504..13734
                     /gene="xseB"
                     /locus_tag="PXB44_000433"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL5280948.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            13740..14633
                     /gene="ispA"
                     /locus_tag="PXB44_000434"
     CDS             13740..14633
                     /gene="ispA"
                     /locus_tag="PXB44_000434"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL5280949.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(14668..15468)
                     /locus_tag="PXB44_000435"
     CDS             complement(14668..15468)
                     /locus_tag="PXB44_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL5280950.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
     gene            complement(15593..16378)
                     /gene="znuB"
                     /locus_tag="PXB44_000436"
     CDS             complement(15593..16378)
                     /gene="znuB"
                     /locus_tag="PXB44_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL5280951.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(16465..17802)
                     /locus_tag="PXB44_000437"
     CDS             complement(16465..17802)
                     /locus_tag="PXB44_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL5280952.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(17812..18300)
                     /gene="mreD"
                     /locus_tag="PXB44_000438"
     CDS             complement(17812..18300)
                     /gene="mreD"
                     /locus_tag="PXB44_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL5280953.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(18300..19355)
                     /gene="mreC"
                     /locus_tag="PXB44_000439"
     CDS             complement(18300..19355)
                     /gene="mreC"
                     /locus_tag="PXB44_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL5280954.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(19457..20509)
                     /locus_tag="PXB44_000440"
     CDS             complement(19457..20509)
                     /locus_tag="PXB44_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL5280955.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            complement(20624..21253)
                     /locus_tag="PXB44_000441"
     CDS             complement(20624..21253)
                     /locus_tag="PXB44_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL5280956.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            21357..22004
                     /gene="udk"
                     /locus_tag="PXB44_000442"
     CDS             21357..22004
                     /gene="udk"
                     /locus_tag="PXB44_000442"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL5280957.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            22019..22600
                     /gene="dcd"
                     /locus_tag="PXB44_000443"
     CDS             22019..22600
                     /gene="dcd"
                     /locus_tag="PXB44_000443"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL5280958.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
BASE COUNT         7026 a         4865 c         4226 g         6511 t
ORIGIN      
        1 attttgtcgc tgtttttgct attcttctgt ttatttttca cattttatta acgatataag
       61 gatattcgat gaattggaaa gcatataaaa aagctattgc ggcagctagc gtggtagcga
      121 ttgcaacggt attagtggcg tgttcgtctt caggccaaaa aggcaaaaca acctcttatg
      181 atgcggaaaa agctaaattt ggagcaaaat ataatggtcg tcactttatg ccttatggca
      241 cacattttca agctggacgt ttaggattaa atgctgctcc tgttaatcaa ctagactttt
      301 tacagcagct ttctaatgtc agagcctata gtagcttggc agattcttat gctatcgctt
      361 acggaaaaat cagtcgttgg atctcatcgg gcggtaatat taatgattta gcaaaatatg
      421 gtattagtac tcgtttaatg cgtggcgaag atggctacca gaatgtgtta atgacagggt
      481 attactcgcc ggttatcaaa gcccgtagaa cgcctcaggg gcaataccaa cacccgattt
      541 atgcgatgcc gtctagcaaa cgtttttcac gggcggaaat ttacgcagga gcattgcaag
      601 gtcagggctt agaactggct tatagtaatt caatgctcga taacttctta ttaggtgttc
      661 aaggtagcgg ttacgttgac tttggtgatg gtcgcttaaa ctattttgct tatgcagggc
      721 aaaatggctt taaatatgcc agcgttgggc gtttattagt agaagatggc gaaattccga
      781 aagagaaaat gtcagtgcag gctatccgtg aatgggggca acgtaaccct agccgtgttc
      841 aaggtttgtt ggagcgtaat ccatcgtatg ttttctttaa aaacgatcct accggtcagg
      901 taaaaggctc tgccggtgtg ccgttagtac cacttgcatc tgttgcatca gatcgtagca
      961 ttgtgccatc aggtagcgtg ttgttggtcg aaatgccgtt gattgatagc aaaggaaatt
     1021 ggaccggcaa acacgaaatg cgtttaatgg tggctcttga tgttggcggt gcagtgaaag
     1081 gccatcactt cgatttatat caagggattg gcgataaagc aggccatatc gcaggtttaa
     1141 tgaaacatta cggccgtgtt tgggtattaa attaatacag ggtaactatg ttaaaaaaac
     1201 tatctgccat attcttactt ctaccgagcg ccgtgcttgc aaataatttg caaaatataa
     1261 ccgcttcaac tgagcctaaa tataaaatct cagaaataga tgtcagaatt ttgatacgcc
     1321 agctcaataa tattgagcaa tgtatctatc ctgagttagc taaacccggt taccagcaaa
     1381 tctacgcaaa ttggaatctg gctgaaaatt taacgatgca atattttgaa tatcagcttc
     1441 taaaagagtt gctcggagag gaaaatcaga aactgatgca aaatgatacg ctttctaccg
     1501 agtatttcca tttactgcat tctcgtttaa atcaccaaaa agcaaatgta gatcccgaaa
     1561 aatgcgatac atttaaacct agatataaag agatttataa aagtatggaa aatgcactca
     1621 ctaagaaaaa tcaatagtaa aaaactcgca gttttcactg cgagtttctt ttatctttcg
     1681 ttatgaattt ctaaattggt ggcttgtttt gccgcctctg cttttgcttt atcgtttcga
     1741 gatcttgcta tactgtctaa atagcattta tctacatcac ctgtaatata ttctcctgta
     1801 aatacagagt catcaaagcg atgaatagtc ggattttcca gttgaataga cttataaagt
     1861 gcttcaaggt cttggaaaat caatttatcc acccctatca tctgtgcaac ctcttccact
     1921 gagcgatcat aagccactaa ttcttcacaa gtcggcatat caatgccata cacattcggg
     1981 tagcgaattt ccggtgcggc agaggcaaaa taaacccgtt ttgcaccagc tgctcgtgcc
     2041 atttccacga tttgttcaga cgttgtacct cgtacaatag aatcatcaac cagtaacacg
     2101 cttttgcctt taaattcaga ggcaatcgca tttaatttac ggcgaaccga gcttttacgc
     2161 tgtgcttgcc ccggcataat aaaagttcga gctacatagc ggtttttaac aaacccttga
     2221 cgatagggtt tatacaacat attagcaata cgtaccgcaa tatcatttga ggtttcagga
     2281 atcgggatca ccacatcaat atcatcgata attcgtcccc attcacgttt aattttctca
     2341 cctaataatt cgcccatatg cactcgtgca gaatatacag aaacgccatc aatgacggaa
     2401 tcaggacggg caaaataaac atattcaaaa atacaaggat tcagttttgg attatcggca
     2461 caaatttgcg aatgtaattg cccatcaaag gtaacataaa tcgcttcacc cggcagcaca
     2521 tctcgcacaa attcaaaccc cactacatca agagccacac tttccgaagc aaacatatat
     2581 tcggttttac cctcgatttc acgtttaccc agcactaacg ggcgaatacc gaatgggtca
     2641 cggaaagcaa ccataccgtg tccgataatc atcgcaatgc aagcataagc accacgaatg
     2701 ctatcattgg ttttacgaac cgtttcaaag atattgtctg ggcttaaatg ctgagtggaa
     2761 tagagatcta aaaagtaagc aaaaatatta agaagggatt cagaatcaga attagtgttc
     2821 acatggcggc gggcttcgtt gtataagcga gctttaagtt ccgcattatt agttaaatta
     2881 ccgttgtgaa ctaaggtaat accgaaaggt gaattgacat aaaatggctg ggcttcagac
     2941 acacttgagc tacctgctgt tgggtagcga acgtgaccaa ttccaacatt gccttgtaat
     3001 ctcaccatat gctcttgctg gaaaacatcg ctgactaagc cgttagcttt gcgtaagcgg
     3061 aaacgatttt catcgtctat ggtgacgata cctgcggcat cttgtcctcg gtgttgaagt
     3121 aatgttaaac catcataaat cgcctgattc accggcgaat tcccaataat accgacaatg
     3181 ccgcacatat agaaaaacct ctttatttag tcgtgtttaa aaaactggaa tttgcttgta
     3241 actgttcaaa gaaccacttc acaataaagt caaaatgcgg aattaatagc gatgatttcc
     3301 ataattccgt ttggcttgct tgggtaaagg tatctaagaa aaataaaata gccgacacaa
     3361 ttaaaatacc acgcaatgcc ccaaaagcac cgcctaaaat acggtcggtg atagacattc
     3421 cgctcaaatc aatcactttg cctaataaac cgttgatcac accacaaaca attaaagtca
     3481 aaatgaacag aatcgccgct gcaataccat tgcgtaaaaa ttctgaattt tgcaaataaa
     3541 ccgaattaat ttgtgtgaga taaccgctta aataaggata aaacgagctt gccacaaaaa
     3601 aggcgattac ccaaccaagt agcgataatg cttcattggt aaaacctcgc cataaactga
     3661 taagcactga aaaagcaatg attccgataa caattaaatc aaccataaca caccttaaag
     3721 cgtaaagctc tcctattcta caaaaaaaga aaacgtttgc gtagcactat ttcgctaaaa
     3781 ataagtgctt tatgcaaata caagcggtta aattcggtaa aaaatttaca caatccgacc
     3841 gcttgtaaaa cctagtctgc aatataacca acatcgtcca acgttggatt atttgctcct
     3901 tgtttagcca gctcatcgca aatctcattt tcacgatgtc ctgaatgccc tttcacccaa
     3961 ctccattcaa tttggtggcg ttgaatttct tggtctaaag caacccataa atctttattt
     4021 ttaactggct ttttatcgct ggttttccag tcatttttct tccagttaaa aatccatttt
     4081 tggatcccgt ttttcatata ttggctgtca ctattgagtt gcaccttaca aggctctttt
     4141 agcattgcta aagcctcaat caccgcccgc aattccattc ggttattggt ggtttgaaaa
     4201 tagcctttgc tcacggtttt ttcgtacccg ttataacgca gcaaaatacc aatcccccct
     4261 ttgccggggt tgcctaaaca agaaccgtct gtaaaaattt caactaattt catcatcacg
     4321 ctttgaaaat aaaagcccca aagcattaac tttggggcag cttttaggct aaattctaaa
     4381 agttatcaga atggagatca actgattatt tttttccaag ttgtggaatc acgctattat
     4441 tagccgccaa taaacctgca tcggtataaa ctgctaattt accacgggta tcgacaatat
     4501 ctaagttacg cattgttaat tgaccgatac gatctgccgg cgtaaacggt gcatcttcta
     4561 ctttttccat acttaaacgt tctgcttcgt aggttaagtt tggcgattca gtgttaagaa
     4621 tagtgaaatc gttaccacgg cgtaactcaa gcgtgaccgt accggtaatc gcttttgcca
     4681 cccaacgttg agccgtttca cgcaacatta aagcttgcgg gtcaaaccag cggccttgat
     4741 acaataaacg ccctaaacgt aatccgttaa tgcgatattg ctcaatcgta tcttcgttgt
     4801 gaataccggt aactaaacgc tcgtaagcga tgtgtaataa tgccattccc ggtgcttcat
     4861 aaataccacg agatttcgct tcgataatac ggttttcgat ttggtctgac attcccaaac
     4921 catgtctgcc accaatgcgg ttagcctcca taattaattc aacggcatca tcaatacgtt
     4981 taccgtttaa cgccaccggc acaccttctt caaaggtaac agaaacagtt tccgctttaa
     5041 tttcaactga ttcgtcccag aatgccacgc ccataatcgg ttttacgatc ttcataccgg
     5101 tgcttaattc ttctaaatct ttcgcttcgt gggttgcacc taacatattc gagtctgtag
     5161 agtaggcttt ttccaccgac attttatagt caaaaccgtt ttcgattaag aatttagaca
     5221 tttccaagcg accgcctaat tcatcaatga atagttggtc taaccacggc ttataaattt
     5281 ttaattttgg attggttaat aaaccgtaac gatagaaacg ctcaatatcg ttacctttaa
     5341 aggttgaacc atcgccccag atattcacat catcttcttt cattgccgca accaacattg
     5401 tgccggttac cgcacgacca agtggcgtgg tgttgaaata cggaataccg ccggttgaaa
     5461 tatggaaagc accgcattga attgcggcaa tgccttcgtg agctaactgt tggcgacagt
     5521 cgattaatcg tgcattttca gccccatatt ccatcgcttt tttcggaatt gcgttgtaat
     5581 catcttcatc cggctgacct aaatttgcgg tgtacgcata aggcactgcc ccttttttac
     5641 gcatccataa cagtgctgct gaggtatcta aaccgcctga aaaggcgata cccactttct
     5701 gccctaaagg taaagcttct aaaattgttg ctgacatacg aattcctata aattttgaaa
     5761 gtaaaaatat tcaaacgcaa acgcttacgt tcaagcaaaa tattctagct gaaaagtagt
     5821 aaaaattcat ctaaaattta gcccgatgat ttgcaaattt tttgtgaaat ttgaccgctt
     5881 ataatttact gaaaacctaa aagcaataga gaaaatcaga atgaaaaaac tactcgtaat
     5941 ccgtaatgat aaaattgggg actttatggt ctgctttccg gcatttgcta tgttgaagca
     6001 atcaatgccg aatgtggaaa ttaccgctct tgtgccaagt tatactgctc cacttgccga
     6061 gctttgccct tctattgata agattattat tgatacggcg aataaaaaag ataaaaatga
     6121 atttaaccgt attttacaag cggttaaaaa tgagcaattt gatgcggtta tttgttttgt
     6181 ttcagactgg cataatgcca aactcacctg gcaaagtggg attaaatacc gcctcgctcc
     6241 agcaactaaa ttattccaat ttttatataa ccaccgttta acccaacgcc gttctcaatc
     6301 agcaaaagcg gaatccgaat ataatttgga tttagcccgt gcttttttaa gcaaacacaa
     6361 tataccgatt gttgaacctc acacgccata cttaagtgtt gaacagcaaa ccgtccaggc
     6421 acaaaaagca aaattatgcg aacaactcaa tattaccacc gttaaaaagt gggtatttgt
     6481 gcatagttca accggtggtt cggcaaataa tttatctata gaacagtatg cagagttaat
     6541 tcaaggtatt ttggcagaat ttgattgcta tgtggtactc accgcaggga aaggcgaaag
     6601 cgaaaaagct cagcagttag ctcaacaaat caatcacaac aacgtggtgg tttatgataa
     6661 aaatgagggg ttacaagatt ttacacattc gcttgcgtgt gcagatttgt ttattgccgg
     6721 ctcaaccggt ccgttacata ttagtggggc gttaaatatt ccaactattg gcttctaccc
     6781 gagccgactt tctgccattc cacgccgttg gaaaccgatt aatgatgcga ataaacacat
     6841 tgcattttgc ccaccgtctg ataaagcctc acaaatgaat ttaactttaa tttctatttc
     6901 tgaggcatta aaaacgatag taccgtttat tcggagaatg tggaatactc cctctctctg
     6961 ataaaattgc taaacgcaat tttatctatc tctctcccac tagagggaga gagtactgaa
     7021 aagcatcatt tccgagtacg attccacaaa tccgcatatt ttacgaaagt agaatgtgcc
     7081 gaaagaactg ccaataaaaa tccttgcttg ccatctaaaa aaccggcttt taatagatac
     7141 attttcacaa aacagccgat ggcgtgagtc acgccatcaa ataagctggc tgttttgcct
     7201 gcatttgccc gttggattgc ccacgcttta gcgtaacttg ccgattttac caaatagtgg
     7261 tgaatatctt tataggtgaa atgcagcaaa tcgccttgta gtttctggat ttttgcctct
     7321 tttgggtaat gtactttttc gtgaaccaat tcatcaccat atttggcaaa ttccgtgcga
     7381 tacaatctca ccacacaatc cggataccaa cccgagtggc gaatttgttt gccaaatact
     7441 tcacttaagc ggctgatttt gtaaacggta tttttttcat catttttgac cgcttgtaaa
     7501 atagattgct tcagttcggc tgtcactcgc tcatctgcat caagccaaag tacataatca
     7561 ctggtcacat attgttgagc taactgacgc tgtttaccaa agccttgcca atcggtgtta
     7621 gtgtagaacc ttgcgttgta ttcagcagca atttgtggtg tttcatcagt actgccacta
     7681 tctaaaatca caatttcatc tacccaatct tttactgtgt caaggcaagc ccgtaaatct
     7741 tgagcttcat ttttgacaat catcgcaaca cttaatgtcg gcatagttta tccttctctc
     7801 tttttgtcgt gtgtttttct gctattcgtt gcattttact ttaaaaatag tggtttttgt
     7861 gctattttct acgccaaatt ttatcaacaa aacaaattaa cggaaaaaga atgtcaatta
     7921 cattaacaac caaacaaaaa caattcctaa aaggcttggc tcatcattta agcccagtgg
     7981 tgatgctggg gggtaacggc ttaacagaag gcgtgttagc tgaaatcgat aacgccttag
     8041 accaccacga actgattaaa gttaaagttg ccggggctga tcgtgaaacc aaacaattaa
     8101 ttattgatgc cattctgcgt gaaactaaag cggctgaagt acaaaccatt gggcacattt
     8161 tagtgcttta ccgccaaagc gaagagaaaa aaatcagctt gccaaaagca actaaagcga
     8221 tctaattgca aaaatatgtt taaattcaac cgcttgtatt caagcggttt tttataggac
     8281 tttctatgtt taaaaaattc tctctttata cccttgcaat gttgctcatt cctgtggtga
     8341 cttggctggc aaattggcac tggtctctgg caattcaata cgctgaattt gatttcgact
     8401 actttttata tttaatcacc gaaaccggct ccgtacctta tgccatcatc acctgtgtgg
     8461 ttttcgcctt attactggcg agggtggttc gcaaacgcta ctctttaatt tttgtgatta
     8521 ccgtgtgtgt cgcttcaatg gtagccactc aagcaatcaa aacagcgatg aaatcaacct
     8581 ttcaagagcc tcgtccattt gtcactgctc ttttccaaga taaaacagac gatttttatc
     8641 aattaacacg cgagcaacgt gggcttgccg taatggaaaa aatcggtgca aatcaaaatt
     8701 ttgttaccgc tcaccaagcc aaagaagtgg gctattcttt cccatcaggc cacactattt
     8761 ttgcggtttc ttggctatta ttggtggtag gtttctgccg taatatgcga ggacaagcgg
     8821 tcgttttggc tcattttttt gcaatcagtt gggcggcgtt aatgctgatt agccgcttgc
     8881 gtttaggtat gcactacccg attgatttat tcaccagtag tttaattgcg tttctaatca
     8941 atttagcctt atttgtgtgg tttgtgcctt ggttggaaag taaaaaatgg tttgctaaaa
     9001 agccttaatt ggcaatttag ccaaatcaat atacaataca aattattgaa taagtaccta
     9061 aaataaggag cttagaatgg aaatgtcgat agaaaaaata gctgaactat taagcgataa
     9121 agaaaaagat gactatccgt ttagtattga ggaaagacaa tttgccgccc aggctagtgc
     9181 tgatacttgg gaggcaatct taaataacac tattcaacct tttgacttca aaaaataggc
     9241 ttgctatgta tcaagttttt attgcagaac caaaagaccc aaacttagag aaaggcttat
     9301 tgaaacactt cattaagtct tatcttactc aagaagaact cgatttcctg cataaaattc
     9361 tcactaattt acccaacatc agaccttttt ggggcgatac cggttacaaa tatatcgtca
     9421 ttgataatgt ttacttcttg tatgaagttt ccgaaagtga aacgaaagtc attctacttg
     9481 gcaccaaatt taataataaa cggaaaactt ttaattttgc gaatacgaaa acccagcaaa
     9541 aatcaatgaa gaaatggatt aatgatttag caatttataa accgcataaa atcaatcaat
     9601 cgcaataaga gagcgtaatt atggcaattt attatggctt agatgtgggc ggcacaaaaa
     9661 ttgaattagc cgcttttaat gaaaaactag aaaaactaca cagcgaacgt gtgccgacac
     9721 cgcagacaaa ttatcaagat tggctgacgg caattaaaac gctggttgag actgccgata
     9781 caaaatttga cgaaaaaggc actgtcgggc tgggcattcc gggctttgta aaccgtgaaa
     9841 ccggcattgc ggaaattacc aatattcgag tggctgacgg caaaacgatt ctgaaagatt
     9901 tagccgaatg tttagggcgt gaagtgcgtg ccgaaaacga tgctaactgc tttgccctgt
     9961 ctgaagcgtg ggacgaaagt aataagcctt attccaccgt attagggttg attatcggca
    10021 ccggttttgg cggtggtttt gtgtttgacg gtaaaattca ttcaggcaga atcggaatgg
    10081 caggcgaagt tgggcatatg caacttaact accacgcact taaattgctc gactgggata
    10141 aagccccaat ttatgaatgt ggttgtggca accaagcctg tttagacacc tatatttcag
    10201 ggcggggttt tgagttactg tttaataatt tagtaggaga aaaaatctcg gcgaaagcga
    10261 ttatcgaacg cttctaccaa caagaaacca aagtggtggc atttgtcgaa aaatatattg
    10321 agctaatggc aatcagtatc agcaatctga ttacgatttt agatccggat atgattgtat
    10381 tcggtggcgg tttatccaac tttgattata tctaccaagc tctaccaaaa gcattaccaa
    10441 aacacttaat gcgttcagcg aaagtgccgg tcatcaaaaa agcaattcac ggcgactcag
    10501 gtggcactcg tggtgcagca gcattattct taaagtaatg aacaaatttg agagagggca
    10561 agcggtcatt tttaataaaa atattgcagc cctctctttt aatttgcctt actgcttagt
    10621 ttcttccggt aatttatata actgattttt atataaataa ctgccaagca atgcgtgtgc
    10681 taacgcctct tttttaaacg attccggctg ttctttaggg gtgagtttat agttagcttt
    10741 atcgtagcta aatccttcta tcggtaaatt tggtcgcatt accaccacat catttccccg
    10801 catcatcgcc tgtgtcgcat catattgcat aaaggcacgg tttgggtcaa tgtcttgcgt
    10861 taaatcatac cctatcatcg gatattgacc acttattccc gccacagaaa gtaatgttgt
    10921 cggcatatcg agctgactca ctaaacggtt atccgttctt ggctgaatgt gttcgcctaa
    10981 taaaagtgcc ggaatatgga agtgttgaat tggcactaaa tgctcgccaa cggctcttga
    11041 atcgtgatct gcaatcacta aaaacaccgt atcttgccag tactctgatt ttttcgctaa
    11101 ctcaaagaaa tgcccgatag cataatcggc atattttgcc gcattatggc gagtctgctt
    11161 cggttgttca tagagttcaa ttttgccgtc cggaaattca aacggatcgt ggttactgga
    11221 actgaatacc aaactaaaaa acggtttacc tgatttgtgc agttgattaa aagtttcatt
    11281 tgccttatca aacagatctt catcactcac gccccaagtt gcggtaaatt tagggttttt
    11341 ataatctttt tcatcgataa tttgctggaa accattaccg tagaaaaagc tcgccatatt
    11401 atcaaaatgc ttctccccac cataaataaa ggaagtatgg tatccttgac gttttaataa
    11461 ttcggcaata ctaaaaaagt tagtttggct tttcgtcagt tttaccacag atcttgccgg
    11521 tgttggtgta aaaccagccg ttaccgcttc aattcctctc accgaacgag tacctgttgc
    11581 ataaagattg gtaaaatacc agccttgttc tgaaagttta tctaaatttg gagtgagatt
    11641 tttccctcct agcgaaccga caaattgagc ccctaaactt tcttctaaga taatcacaat
    11701 gtttttaggc tttccttgat aagtcgcttt attttgcgtg agggtgggaa tttcatctga
    11761 aatataatct gaagctggtc gattacgaac ctgcttaaca atgttgaaca tttcctccac
    11821 agacattttt ccatactgtt cggaagattt atcttcatct ttcaattgct gaaccgcata
    11881 aagcacggaa taacctgaat ttagcaccag tgaattgaca agcggatcgc ttgaaaatgc
    11941 caccattgcc ggattaatcc ctcgatgggc aaaactggaa cgcccaccaa caaaaactat
    12001 tgcagcaacc aataaagcca gtagcggacg cattttccag ctcgggaaat gcaaattttt
    12061 ggttgcccaa cccgataatt tccaataaat gatgagagaa atcatcgcaa tgccaatccc
    12121 acaaataaca gcccataaat gtccgttaat cagcattgtg aacacttctc tcggatggac
    12181 caaatactca atgaaaagac gatttgggcg aaggtcaaag gtttcaataa aattcggtgt
    12241 ggcgacttcc ataaaaagaa taaatacact accgacagtt agccaaattc ttaaaacagt
    12301 ctgccaaatc gaacccagta catttttatg aaaacacaac acgctaaata atgccggtaa
    12361 gccaaatagc caacatagcg aagatatatc aatgcgaata ccttgtagta ttaatggtat
    12421 ccatccatca acagcggata ctcgatcaga ttgccaaacg cctaacacta aacgggataa
    12481 ggtgaggata actaaattaa tcccaacaaa tagtaggatt ggaaatagca cagttcttga
    12541 ttgactgcgt tggtacattt tgaatctcct tttaaaaaaa tatcggcata ttataattga
    12601 ataatgacag gatcgcctac ttttatcaca ccactgtttt gtggcacaag gtgaataccg
    12661 aacaccggct tacctttttc atcgcaatgc ttttctttta aggttctaaa cggctccgat
    12721 ttggggtcta actcaagcgt gtttaaatcc cgtgtaatca aaatgcaacg agtacataat
    12781 gccgctttgg taaatagcac ttcgccaatt tgaacttgtt gccactgctc ttcttcaaat
    12841 gccacattgc catcaatcac aatattcggg cgaaaacgag ccatttcgag tggaatcggc
    12901 gaccattgtt gcacttgctc aagcgatttt agagacatca aactcaccgg gttgctatct
    12961 gcaaaactca ttgggtgatc ggcaaaattc gcaacaattc gttggctttg cgagccaagc
    13021 caacgtagtt gcacagccct accaaaaatt tcactcagcc attgattgac cgcttctgcc
    13081 gccactttgg cattaaaatg tgttccccac acttcactac tctgctcttc cacaaaatct
    13141 tggtaaagtg caacgcattt ttgaccgctt gtatgggtaa tcattacccc tgtcgcaatc
    13201 gggaaagccg ctagacggta taattccgga tctttacggg cagtaataaa tttgccgtta
    13261 ggctcggcta gcataaattc acggtcaaaa tttaagcctt tcggttcaac aaatgcctgt
    13321 gaaacttgaa tagcttgagt tgattttatc gggtaaatat tgagctcggt aacttgcata
    13381 gaaaatcctc catttggaaa taggataaca atacctgcaa gcggtcgaaa agtaaaagga
    13441 atttgcaaat cccttgtgag aacggtaaac ttgagcaact tttacagcaa taggtcagca
    13501 acaatggcaa agaaacccac tcaagatttt gaaagcacac ttaaagaatt agaagcaatc
    13561 gttacccatt tggaatccgg tgatttaccg cttgaagaag ccctcaacga atttgaaact
    13621 gcaatcaaat tagtgcaaca agggcaagaa cggttacaaa aagccgagca acgtattcaa
    13681 atcttattaa ataaaaacga tactgcccca cttagcgatt acgaaagaga ataatcacaa
    13741 tgagctatct attagcaacc gatctgactc acctgcaaca acggattaac cattttctcg
    13801 ctgaaaaatt aaaaacaacg gatagcgttg gttcgccttt agtagaagca atggcttatg
    13861 cggtattgct aggcggtaaa cgggtgcgac cgtttttaat ctatgccacc ggcagaatgc
    13921 ttggtgtgcc gttggaaaaa ttagatcata gtgcggcggc aatggaggct attcacgctt
    13981 actcgctggt tcacgatgat ttaccggcaa tggacgatga tagattacgc cgtggcaagc
    14041 caacctgcca tattgccttt gatgaagcta ccgctatttt agccggtgat gcgttacaaa
    14101 gttttgcgtt tgaactgctg gcaaccgatc cgcatttgac cgaccgagaa aaagttgttc
    14161 aagttacaga attggcaacc gcttccggtg caaaaggaat gtgcttaggg caaagtttgg
    14221 atttgattgc ggaaaataaa gcggtgagct tggaagagct tgaattaatt catcgcaaca
    14281 aaaccggggc attgattcta tcatccgtga tgatgggatt aaacctttcc gaatattcgc
    14341 aaaatgtagc cataaaacaa ccgcttgtaa actatgccaa agcgattggc ttagcctttc
    14401 aggtgcaaga tgatatttta gatgtcattg gcgaaacaga taaaatcggc aaaatagtgg
    14461 gttcagatga acacttaaat aaaagcacat acccgaaatt gttgggctta gacggggcaa
    14521 aacaaaaagc cgaaatgctt tatcaaaccg ctctccattc gctggaacag ttgcctttcg
    14581 ataccaccgc attaaaagag ttagctaatt ttattgttaa gcgtgaaaac taagaaaaaa
    14641 cctagccgaa gctaggtttt ttagtgatta aacgtgaggc tgagcttccg gcaatgtgcc
    14701 ttcaatttca tataccacat cgccgtcttt aaagaaaaca tagccaccgt ttttcatttt
    14761 ggtccagttt tcatttttgg taagtggctg agtggcaata atcgtcactt tatcggtaat
    14821 tttggtatat tgactaaaat caatcgacac atcatcatca cgcaaggctt tgccaaacgg
    14881 tgctttacgg caaacgtaat gcagattagt tgtacaacgg gcaatcatcc aatgaccgtt
    14941 ggataaaaca aaattaaata ccccgtgttc cgcaattctt gaggtgattt gcaccacggc
    15001 atcaaaaatc tctttttcgc ttggtttagc cggatacatc tcttttaatt ccgacacaat
    15061 acggcaaaat gccgcttcgg aatcggttgt accaatcggc tggtagtggc actcctcgca
    15121 taccccaaca ccttccactg tgccgttatg ggcaaacacc cagttctcgc cccataattc
    15181 acgaatgaag gggtgggtat tttccaaatt cacatcacca cgcgtagctt tacgaatatg
    15241 tgcaataaca ttgagcgatt taatttgata ttggctgatt gcttctgcca tcggcgatga
    15301 tgctcccggg cggttatcac gaaaaacccg aacccctttg ccttcaaaaa aggcaattcc
    15361 aaacccatca gtatggtgat ccgtcagccc accccgacgg cgaaatccat caaaagaaaa
    15421 agtaatatcg gttggcgtat tacaattcat tccaagtaat tgacacataa tttgcttctt
    15481 aaaaccaaat aatttacaac aaaaaataat tgttacattt aaacagattg attagaaaat
    15541 tcaatgctct ttacataaaa atcttatgct ttattttcat acattatgct gtttaatggt
    15601 attctttttt gaatagcgat aatagaaaaa tcaagccggc acaaatgact accgaaggcc
    15661 ctgctggtgt atctttccaa ctggataaca ataaaccgcc actgactgca ataatactga
    15721 agaaaatcgc aatcaacacc attttttcag gcgttttagc aaagcgtctt gccgtagcag
    15781 acggaataat cagcagtgag gtaataatta aagcaccgac aaatttcata cttaatgcga
    15841 tagttaatgc ggttaaaatc atcagcaata aacgtaagcg agccacattt aatccttcaa
    15901 tttgagcaag ttccgggctg atggtaatcg acaacaaccg cttccaaaac agcattaaaa
    15961 tcaaacaaat tagggttgct ccgattccga taaacaccac atcagaaaaa ttaattgcca
    16021 gtaaatcgcc gaataagtaa gacatcaaat ccactcgtac attatccaac aaactaatgg
    16081 taattacccc gagagaaagg ctagtatggg cgataatccc caacacggta tccaccgaat
    16141 aattggtttt ttgctccaac caaaccagca ataatgcaag aagaatagtc attccaatta
    16201 cagcaagata cggatcgagt tctaaaaaaa tccctaatgc cacgccgagc aatgcagagt
    16261 gcgaaagtgt gtcgccgaaa tacgccattt ttcgccacac cacaaaagcc cctagaggtg
    16321 cggtaataaa ggctaacaaa acaccggcaa gccaagccgg aaataaaata tcaaacatat
    16381 tggttctcaa aggcaagtaa aacaagcggt tattttttcg gaaaaatttg caaatttttt
    16441 gctgatttag accgcttgtc atatttattt ataacgttga atcgggctgg cttgatcctg
    16501 tgctttcagc cacgccattt tttctttaat ctgtttttcc attccccgct cacttgggaa
    16561 gtagaattcg gtatctttca gctcaggcgg gaaataattc tccccggcgg cataagcgtt
    16621 tggctcattg tgggcatagc gatactcttc gccatagcct aaagatttca ttaattgagt
    16681 gggagcatta cgcaaatgcg ccggcacatc gtagtctttt gcctctttag cgaggcgctt
    16741 tgcttcatta aaagcttgat agaccgcatt gctctttggg gcaactgcca aataaatgat
    16801 cgcttgggca atcgctcttt caccttcata agcccccact cgggtaaagc aatcccaagc
    16861 cacaatcgca atttgcattg ctcgagggtc ggcattacca atatcttcag aagcaatcgc
    16921 aagcagcctt cttgccacat aaagcggatc accaccggcg gttaaaattc gggcatacca
    16981 gtataatgct ccatcagccg atgagcctcg aatggattta tgtaacgcag aaatgagatc
    17041 atagtaacga tcaccgcctt tatcaaaacg agcttgtcgt tcgcctaaca cttcggtcag
    17101 caaactttta ttcagcactt tccctttagc tgaatcttgt gccatatcta ccattaattc
    17161 caaacagttc aaggcaaaac gggcatcacc attaacataa tcagcaagca atgccagtac
    17221 atcatcttca agtaccaaag tttcattgcc taaaccacgt tcggtatcgg ctatcgccat
    17281 ttgcaaaatt tttagaattt cgaccgcttg taacggcttt aaaatataaa ttctggctcg
    17341 ggaaagcagt gcgttattta gctcaaaaga gggattttcg gtagtcgcac caataaaaat
    17401 aatagtgccg tcctcaatat gcggtaaaaa cgcatcttgc tggcttttgt taaagcggtg
    17461 aacttcatcc acaaaaagca acgttcgtct gccggcttgg cggtttaatt tggcttgttc
    17521 aatcacctct cggatctctt ttacaccgct ggtcacagcg gaaatacgtt caacttccgc
    17581 atcaagatga tgggcgataa tttcagctag ggtcgttttt ccggtgccgg gcggtcccca
    17641 aaaaatcata gaatgactat gtccggcttc aatcgctctg cgtaacggtt tgccttcgcc
    17701 aattaaatga gattgcccga catattcagc caagtttcga ggtcgcattt tggctgcaag
    17761 cggtcgaaaa tcttcactaa aatcaaaaga caaactactc attttaacct cttaacgtaa
    17821 ccgcagttgg cgacgaatat gacgcattaa taagaaaatc caagaccaaa gcacaccact
    17881 gattacagca cctaacaatt cctgtgaatt aaattcggca ctgtgtaaaa tgtattcaat
    17941 cacgaaaata aagaaacgga tcagcacaat atatgccatt actaataggc tttgtagcca
    18001 tagcgataag tttcgcagaa tcagatggta tttcgcaaca aaataaatcg ccaccgataa
    18061 cactaaggcg tggatgccta aaatcgagcc aagaatcaga tcccagacga tgccggcaat
    18121 aaaagccgtc cccacactca ttttatcagg taatgccaat gcccaataaa ttaacaccaa
    18181 aacgacccaa gtagggcgta gcccttggaa tcctaccggc caaggcataa tctcaagtac
    18241 aaaagcaacc acaaaaatcg caattatcac aaggatctga aaaatcgggt ttgttctcat
    18301 tattccgctc cttcttccgt atgatagttt tgttcatttt ccgcttctgg ttgtgctgcc
    18361 ggattctcat ttatctcatc attctctatt tgttcttctt ttgcctctag ctgcacttca
    18421 gttttcttgt tagacatctg ttttaaacgt tcaaatggat tcacgctatt acgtctttct
    18481 tcaacaactt cacgtacttg cgctggcgac aagctttggg agtagttttt ctcttccgcc
    18541 gtcggccaaa gcagtaataa gtaacgtaag cgatcaaatg aggctaacgg acgagcaaca
    18601 attctggcaa attgactttt ggtatcattt tcaaccgttt ctacaatcgc caccggatat
    18661 ccctcaggga aacgtccacc taagcctgaa gtcaccagca catcaccttt taccacatca
    18721 atagaacgag gcaagttatc aatgaccaat tcattattca cccctgtacc actggcaatg
    18781 ccacgtacat cattgcgtag cacttgaaca ggaatagcat gagtaatatc agagagcaat
    18841 aaaacacggc tagatttttc ccctacagaa ataacttgtc caacaacacc tctttcatca
    18901 ataataggtt gcccgacaaa agcaccgtct aaacgccctt gattaatcac tacttgctga
    18961 cgataagcat ccatctcagc agctaatact tcagcaattt ttttatactc atcttgccga
    19021 agcggagaac tcaataacaa tcttaaacgc tgattttcaa cctttagctg atcaagcaat
    19081 aataaatcgg cacttttttc tcttagctgc tcttttaata aattattctc aagctgtaat
    19141 ttattattat caataaaatt gccacttacc ccatctaaaa ttgaacgggg ggtattagca
    19201 aaataatata atccacttac cgcggtttct aatagattac gcgcttgaat cattaaagag
    19261 ctacgcccat cggaaataat cagcataata gaaaatatca ctgcgataat caggcgcatc
    19321 cctaaagagg gagctttagc aaaaataggt ttcattagag aaatatatgt gaatttattg
    19381 ggaatctata acgaaaattg acaatagaat caatccattg ccaacctcca tttaaacagc
    19441 tgctaagaga aatgtattaa tcatcaatac taaatacatc accaccgtgc atatcgatca
    19501 tttctaatgc tttaccacca ccacgagcta cacaagttaa tggatcttct gctactacga
    19561 cttgcacgcc agttgaatca gaaagtaaac gatctaaatt atgtaataat gcaccacctc
    19621 ctgttaacac cataccgcgc tcaaaaatgt ctgctgccaa ttcaggtggg cattgctcta
    19681 agactgattt aactgcctct acaataccat ttaatggttg ttcaattgct tctaatacat
    19741 gtcttgagtt aagctcgaaa gtacgaggag cgccttcagc taaattgtgt ccgtgtactt
    19801 ccattgtttt aacatcatca tcggattcaa tcttagcact agcaatttct tgtttaattc
    19861 gctctgccgt tgcttcacca atagcagaac caaaatgacg acgtacataa gcaataatag
    19921 cttcatctaa cttatcacca ccaatacgca cagaagttga atacacaatg ccgtttaaag
    19981 aaagcactgc cacttcggtt gtaccgccac caatgtcaat taccattgac cccattgctt
    20041 cgtgaaccgg caaacctgca ccgattgcag ccgccattgg ctcttcaatt aaatagactt
    20101 cacgcgctcc tgctccaatt gcagattctt taattgcacg gcgttccact tgtgttgcac
    20161 ctgctggcac gcacactaat acacgagggc ttgggcgcat aaagttatta ctatgtactt
    20221 gtttaataaa gtgttgcaac attttttctg ttacaaaaaa atccgcaatg acaccatctt
    20281 tcattggacg aatcgccatt atgctttttg gtgtacggcc tagcattaat tttgcatgtg
    20341 agcctaccgc cgcaatactt tttagcgatc ccatacgatc ttgacgtaca gcaaccactg
    20401 agggttcatc tagcacaata ccttgacctt taacataaac cagtgtattt gctgtcccga
    20461 gatcgataga aagatcgttt gaaaataatc ctaaaaattt tttaaacata aaagaatccg
    20521 ttaaaataaa attattcaaa aataagcaca ttttatagtg cgaacattgc tactaatata
    20581 gcaaaaaagc ccgtcattca tcaatgaaaa aatttaaact ccactagaaa atttgcagag
    20641 caagttgata agtataaatc agtaaactaa aagcgataaa gtacataatt agcccggtaa
    20701 cagcatctaa aatttgccaa gttcttcgct ttgcaaaata aggggaaagt aagcttgctc
    20761 cataacctat gccaaaaaac caaataaatg agcaaagtaa tgcacctgct aaaaagtata
    20821 ttttaccgtt aaaatctaat ttcccaccga tactgcctac aatcacaacc gtatcaatat
    20881 aaacgtgagg atttaagaat gtaatcgcaa aggtaatcag taaagccttt tttaagctcg
    20941 ttgcattctc tccacttgcc acaaggtatt caccactttt aaatacgctg ataaatgaac
    21001 ggctaccgta gcttaataga aaaatggccc ctaaaagcga aataatgaaa cttacaacag
    21061 gcaattccgc aattaacgtg cctaagccta gcacacctat cgtcattaaa aatacatcac
    21121 ctaaaaaaca gagcaatgca atccaaaata tgtgttgttt taaaatccct tgctttaata
    21181 aaaaggcatt ttgtgcgcca atagagacaa ttaagccaaa acacactata aatccttgaa
    21241 tgaaaatatc cattttttct ccaaaatcgc ataacaagcg gtcaattata caaaaaattt
    21301 tgcaaaactt cggtataatt tatcgaatac tcttttgact gaataggaaa ttttttatga
    21361 ctgatactag ccagacttca tcttgcgttg tcattgcgat tgccggcgca tctgcctcag
    21421 gtaagagcct gattgcctca accatctata aagaactcaa aaatgaattg ggaacagata
    21481 atatcggtat tatttccgaa gatgcttatt atagagatca atctcatcta acaatggaag
    21541 aacggattaa aaccaattac gatcacccta attcaatgga tcatcattta ttggttgaac
    21601 atttacgcca attaaaacaa ggtaatgcga ttgaaattcc agaatatgac tataccgaac
    21661 ataatcgtaa aacgaccacg aagcactttg aaccaaagaa aatcattatt ttagaaggta
    21721 ttttattgct gacagatgag aatattcgca atgaaattaa tgtttctatt ttcgtagatg
    21781 ctcctctgga catctgcttt atccgccgct tacaacgtga tatggtggaa cgtggacgtt
    21841 cgatggaatc agtgattagt caatatcgca aaaccgttcg cccaatgttc ttacaattta
    21901 tcgaaccctc taaacaatat gctgatatta ttgtgccaaa aggtggcaaa aaccgtattg
    21961 ccattgatat tcttaaagca caaattaagc aattactaaa ataaaatagg agctaaatat
    22021 gcgtttatgt gatacagata ttaaacgtta cttagatgaa ggtattattg ccataacgcc
    22081 tcgaccttca gacgataaaa tttctggcgc cactgcagat gtgcgtttag gtaattcgtt
    22141 tcgtgtattt cgtgaacata aaacgcccta tattgattta agtggcccta gagaagaagt
    22201 tgccgctcag ttaaataaag tgatgagtga tgaaattatc atcggtgatg atgaagcatt
    22261 tttccttcat cccggtgacc ttgcactcgc aacaaccctt gaatcggttt ccttaccagc
    22321 caatatcgtt ggatggttag atggtcgttc gtcacttgct agattaggct taatggtaca
    22381 cgttaccgcc cacagaatcg atccgggttg ggaaggcaaa attgtattgg aattttttaa
    22441 tgctggaaaa ttacccctag ctttacgccc taatatggca attggcgcat taagctttga
    22501 gattttaagt ggtgatgctg caaaacccta taatgcgcga aaagatgcaa aatataagaa
    22561 ccagcaaagt gccatttcaa gtcggattaa tcaagattaa tatagtacat gccttgcaat
    22621 catacatt
//
LOCUS       DANLUA010000013        22438 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLUA010000013 DANLUA010000000
VERSION     DANLUA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22438
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(39..2228)
                     /gene="yccS"
                     /locus_tag="PXB44_000444"
     CDS             complement(39..2228)
                     /gene="yccS"
                     /locus_tag="PXB44_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL5280959.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKSVR"
     gene            complement(2272..2730)
                     /locus_tag="PXB44_000445"
     CDS             complement(2272..2730)
                     /locus_tag="PXB44_000445"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL5280960.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2856..4130
                     /gene="thrC"
                     /locus_tag="PXB44_000446"
     CDS             2856..4130
                     /gene="thrC"
                     /locus_tag="PXB44_000446"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL5280961.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4209..4943)
                     /locus_tag="PXB44_000447"
     CDS             complement(4209..4943)
                     /locus_tag="PXB44_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL5280962.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKYIGGSDELAAYFAK"
     gene            5058..5942
                     /gene="oxyR"
                     /locus_tag="PXB44_000448"
     CDS             5058..5942
                     /gene="oxyR"
                     /locus_tag="PXB44_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL5280963.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5945..6577
                     /gene="fabR"
                     /locus_tag="PXB44_000449"
     CDS             5945..6577
                     /gene="fabR"
                     /locus_tag="PXB44_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL5280964.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6604..6903)
                     /locus_tag="PXB44_000450"
     CDS             complement(6604..6903)
                     /locus_tag="PXB44_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL5280965.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWSALSEHHQLALEWLKLSTAYITKE"
     gene            complement(6992..10807)
                     /locus_tag="PXB44_000451"
     CDS             complement(6992..10807)
                     /locus_tag="PXB44_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5280966.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(10770..11255)
                     /locus_tag="PXB44_000452"
     CDS             complement(10770..11255)
                     /locus_tag="PXB44_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5280967.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(11264..13423)
                     /locus_tag="PXB44_000453"
     CDS             complement(11264..13423)
                     /locus_tag="PXB44_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5280968.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(13652..13852)
                     /locus_tag="PXB44_000454"
     CDS             complement(13652..13852)
                     /locus_tag="PXB44_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL5280969.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
     gene            14216..15370
                     /locus_tag="PXB44_000455"
     CDS             14216..15370
                     /locus_tag="PXB44_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL5280970.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(15418..16407)
                     /locus_tag="PXB44_000456"
     CDS             complement(15418..16407)
                     /locus_tag="PXB44_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5280971.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
     gene            complement(16567..17931)
                     /gene="mpl"
                     /locus_tag="PXB44_000457"
     CDS             complement(16567..17931)
                     /gene="mpl"
                     /locus_tag="PXB44_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL5280972.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(18227..18982)
                     /locus_tag="PXB44_000458"
     CDS             complement(18227..18982)
                     /locus_tag="PXB44_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL5280973.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            19137..19454
                     /gene="metJ"
                     /locus_tag="PXB44_000459"
     CDS             19137..19454
                     /gene="metJ"
                     /locus_tag="PXB44_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL5280974.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            19502..19732
                     /locus_tag="PXB44_000460"
     CDS             19502..19732
                     /locus_tag="PXB44_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL5280975.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            19725..21149
                     /gene="panF"
                     /locus_tag="PXB44_000461"
     CDS             19725..21149
                     /gene="panF"
                     /locus_tag="PXB44_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL5280976.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(21226..22416)
                     /gene="nhaA"
                     /locus_tag="PXB44_000462"
     CDS             complement(21226..22416)
                     /gene="nhaA"
                     /locus_tag="PXB44_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL5280977.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
BASE COUNT         6388 a         5077 c         4244 g         6729 t
ORIGIN      
        1 tgaagcatgt cacattttat gatttaatta taggctcact accttacact cttgagctgt
       61 aatatactat ttaactgcgg taataactgc acgatcagtc tgagttgttg tatcaacact
      121 aaggcgaggt cattttgctt ttgctgattt tcttgctcaa attgggtgag gtcttggtcg
      181 atagcggtca atttttccga taattttgca aatgaacggc tctctgaggc aatttgcccg
      241 atgatgtttg ccacctctct ggcgtgtgag aagaacattg cggaaaaatc cacttgttga
      301 ttaatctctc ggctagcaat acggtaagcc cctaaggtgg aaatataacc cagcaaggtg
      361 taagcaatac ctaacaattt tggggcgaag gttaaatcgt tcgcatattt ttgtggttcg
      421 ctgtacatat tggtaatcgc attactcagt gccgataaat ggttatgcac atcacggcgg
      481 gcaatacggt aaggcagttg ctcgttataa ccaaactgca actgggcgat aatatggcgt
      541 aaatattgcc cactggcttt caagccgtta tgtaagttac gttgtaaatt taaatacttc
      601 caatccggcc ataagaatgc cactgccagc cacgcaatcg ctgtacctag taaggtatca
      661 agcattcgca ttaagatgcc gctttcaagg ctcacgcctg caatatcaag gcttatcatt
      721 accaacacag tgatataaaa ggttgaaaag ccataatgta ccaagcggaa aaaggtataa
      781 agcgaaccgg tcagtacgat tagcccaagc tgggcttcga ggctgagcgc cacgttaaac
      841 agttcaaaag agccaccaac cagcacgccg aaaattgtgc cgatcacacg ctgaatcagt
      901 cgggtcttag tagcagtgta attcggttgg cagacaaaaa tggctgtgag taaaatccag
      961 tagcctttgc tatcaaagcc taaaatcggc acaatcgcac tgcacaacag caccactatc
     1021 gacaaccgaa cggcgtgtcg aaacagttgg gattcaaagg ttaaatgggc tttaattgct
     1081 tggctgatat tgcttaagcc ggtaatattt tcggcgatca agcggtgaga ttttgtgaaa
     1141 tttttactta acggttcaat atgctcacca ctgcttgagc cttgttcaat ctgggctagt
     1201 tgccgctcaa tattgcctaa attttctgca atagcacgcc agcgataagc acttttcaag
     1261 ccccgttctt gatgataatt caatgaatta agcaagccct gcaaggcttt ttcgccacga
     1321 acgccgtgtt catatacttc tctatggcgt aacgacattg caattttttg acacgccgca
     1381 gcctgtaatt ccagcacccg ttgaaagcgg aacattaaat cactgttact taattgttgg
     1441 aacagttcgt gatattgata atggctggaa cttgcccgct ctaaaatatc ttgagcggtg
     1501 aaatagtaac gcaacattcg ctgtgtccgt tgctgcttgt gctggcggct taggcggtag
     1561 aatagggcaa ctctggtttt atcaaaggca gccatcacct cactattggc tttcgccaag
     1621 gctagttgtt tggcagctaa atcgtcatca tcagggtcaa aataggcaga tttcgccagt
     1681 aaataatggc tcaacgcaag gtaggcggac gctaaattct cctgcacggt tcgattcggg
     1741 aaaatcaggt acaccaaaat accgaccaaa ccatagagta aggtgccaag taagatcatc
     1801 accacattgt gataccacaa tacttcgggc gtataagcca ggctgttata aaccgccaca
     1861 atcagcgtgc caaaagcaat ggtactatag cgttgcccga ttgcccctaa cattaccagc
     1921 aaaaaggtgc aaaccaccgc caccggcaca aacagccagc ccaaatggag cgacacactc
     1981 gccccaatcg aggaaagcga gaacgccact aggctgataa tcagattttt cgctcgtccg
     2041 attaggctgt tgtctaaatc caccaaaccg ccggcgataa tgcctaacat caacggcatc
     2101 gcaaaatcag aaataccgag ctgccaaatg ctgaccgccg caatgtttag cgaaataaac
     2161 atcggcaggg ttttaattac attttccgaa agccaactgt gttgtagata tcttaaaaat
     2221 ttattcatat ttgctcattt cgggcaggca caggacctgc ccctacctac actaccccaa
     2281 acgttctgcc aaatagccat cataatccgg cacattcacg ctgattgctt gattgaacaa
     2341 atccgcattt aacagaaaat tagcggtggt ttcgttaatc gccaccggaa tattccaaac
     2401 ggtcgcaatc cgcatcaggg ctttcacatc aggatcgtgc ggagcggcat tcatcggatc
     2461 ccaaaagaac accagcagat caattttctt ttctgcgatc agcccgccta attgctgatc
     2521 gccccccatc gggccgctta acaatgaagt aatttcaagc cctgtttctc tggcgagcaa
     2581 atgcccggta gtgccggtgg catatagctt atgcgaggca agttgggctt gatgagtttg
     2641 tgtccaacga attaaatctt gtttacagct atcgtgagcc actaaggcaa tctgtttttg
     2701 ttttgcgatg gttctgacgg tggttttcat ttttagctcc tttgcaaaat ttttcacaaa
     2761 tcataccgct tatttttcgc ttttgcattg actaatcacg gcttatggct aaaatcaggt
     2821 aaaaattcgc attttaaaac aaaggattaa ccgcaatgaa cttatacaac atcaaacacc
     2881 ctgaagaaca agtcaatttc gcccaagcgg tacgccaagg cttaggcaaa aatcaagggc
     2941 tatttttccc tgaagtgtta cctaaattag atagcattga tgaattattg gatttacctt
     3001 tggttaaacg cagccaaaaa attctttcag ccttaatcgg cgaagagtta ccgcaagaaa
     3061 ccttaaatgc aatggtaaaa aatgcgttca ctttcccggc accgcttgcc aaagtcaatg
     3121 atgatattta tgcgttagaa ttatttcacg gtccgacttt agccttcaaa gattttggtg
     3181 ggcgctttat ggctcaagcc ttagccaatg tacgaggaga cggcaaaatt actattttaa
     3241 ccgctacctc aggtgacacc ggtgcggcgg tggctcatgc tttctatggt ttggaaaata
     3301 tcaacgtggt cattctttac ccgaaaggca aaatcagccc attacaagaa aaattattct
     3361 gtaccttagg cggtaatatt cgcactgttg cgattgaatc cgactttgat gcttgccaag
     3421 ccttagtgaa acaagccttt gatgatgccg agctacgcca agcgattggt ttaaattccg
     3481 caaattccat caacattagc cgtttactgg ctcaaatttg ctactacttt gaagcagttg
     3541 ctcagttacc aaaagaaaaa cgagataacg tagtggtttc agtaccaagc ggtaatttcg
     3601 gtaacttaac cgccggctta attgccaaaa cattaggttt accgattaaa cgttttattg
     3661 cctcaaccaa tgccaatgac accgtgccac gctatttaca atcaggcaaa tgggaaccaa
     3721 acgcaaccgt tgcaaccctt tctaatgcaa tggacgtaag ccgcccgaac aactggccac
     3781 gagttgaaga attatttaaa cgcaatggct ggagtttgtc tgaattgggt tctgccgcat
     3841 taaacgatgc cgaaaccgaa gcggctttga aagctcaata tgcagaaggt tatctgtgtg
     3901 agcctcacgg tgctattgct taccaaagct tgaaagatca gctacaacaa ggcgaaaccg
     3961 gtattttctt atgcaccgct cacccggcaa aattcaaaga gtcggtggaa cacattttag
     4021 cgattgaatt gccgttaccg gaagcattag ataagcacaa caaactgcca ctgttatccg
     4081 atgaaatggc agcagatttt gcaacattgc gtgcatattt gttgaaataa atattcaata
     4141 atagaaaagg gattaagtat taaacttaat ccctcttttt atgcattaca agcggttata
     4201 tttgcctatt attttgcaaa ataagcagct aactcatcac taccgccaat atatttaccg
     4261 ccgataaata cttgtggcac agaggtttta cctgtaattg ctcgtacaga aattgtgctt
     4321 gcatcacgac ctaacacaat ctcctcaaaa gtatagcctt gctcttttaa taatgctttt
     4381 gcttttgcac agaacaggca acccggttta gtgataatcg aaacagactc tttagctgtc
     4441 caatttggac ttaaatattt aatcattgta tcggcatctg atactttaaa cggatcgccc
     4501 ggttcttgtg gctcaataaa cattttttcc acaataccgt ttttcaccag catcgaataa
     4561 cgccaagaac gtttaccaaa cccaaggtca tttttatcaa ctaatagccc cataccttct
     4621 gtgaactcac cattaccatc aggaataaca gtaatatttt cagattcttg atctgctttc
     4681 caagcgttca ttacaaaggt atcattaact gacatacaga tgatatcatc tacccctaat
     4741 gccttaaatt cacaagctaa ttcattatag cgaggcaaat gtgttgatga acaagtcggt
     4801 gtaaatgctc cgggtaatga aaacacaacc accgttttgt tattaaataa ctcatcggtt
     4861 gtgacatcaa cccaagcatc gttttgacgt gtacgaaatg ttacactggg tactttctga
     4921 cccgtcatat ctttaaaaga catattaatg ttctcctata gaattatata ttacaaaaat
     4981 cttaggcatt atactcacaa atatggcata attcagccta tcagaacaat aggaaaaatt
     5041 taaattggag gaatcaagtg aatattcgag atttagaata ccttattgca cttgcagaac
     5101 ataagcattt ccgtcgtgca gcagaatctt gtaatgttag ccaacctact ttaagcgggc
     5161 aaattcgcaa attagaaggc gaactaggca ctattctgct tgaaagaacc agtcgcaaag
     5221 tcctctttac gcaagcaggc ttaacattag ttgaacaagc caaggtagtt ttacgtgaag
     5281 taaaaattct aaaagaaatg gcaagcaacc aaggtaaaga gatgtctggc ccgcttcatg
     5341 ttggtgtaat cccaaccttg gggccttaca tttcgccaat tattgtgcct gcattaaaag
     5401 cagaatttgc agatttagag ctgtatattt acgaattaca aaccaaccaa ttattagatc
     5461 aacttgaatc cggccagctt gattgtggca ttatctcatt tgtaaaagag agtgaatctt
     5521 ttattgaagt gcctatcttt aatgaagaaa tgtacttggt agttccagaa caacacgaat
     5581 gggcagcgct taaaaaattc gatatgaatt tattaaaaaa ccgtgagttg ctctttttag
     5641 acagtggcca ttgtttacgc actaatacgc tagactattg tctatccatt ggagcaaaag
     5701 aaaatccaca ctttaaagct accaaccttg aaaccttacg caatatggta gcagcaaatg
     5761 ttggcctagc attgattcct aaattagccg ctaaaccgat taaaggagta cattatattc
     5821 cacttgaaaa cccgaaaccg tttcgagctg tgggaatggt gtatcgacca ggttcaccat
     5881 tacgcgcccg ctatgagcgt ttagctcaag ccattactaa aattatgcaa tccgaggcgt
     5941 aaaaatggca aacggtatga gtgtgcgcgc aattcagaaa gaaaaaacac gcagagcatt
     6001 agtagaagcc gcctttaatc aattaagctc agaaaaaaat ttctctaatc tcagtttgcg
     6061 tgaagttgca cgtgaagctg gagtcgcacc gacttcgttt taccgccact ttaaagatat
     6121 ggacgaacta ggcttagcaa tggtggacga atccgggcta ctattacgcc aattaatgag
     6181 gcaagctcga aaacgaattg caaatggcgg aagtgttgtc gtggtttcag tggaaacatt
     6241 cttcgaactg attgccgacc gccctaacgt tttccagcta ttactacgcg aaagctccgg
     6301 tacatcgcaa gcgtttagaa ccgcagcatc acgcgaaatt caacattttg tagctgaatt
     6361 ggcggactat attatgagca aagataatca cagtaaccga gaacttgctt atattcaatc
     6421 ggaaggttta gtcacgctag tatttactgc cggttctcat gctttggata tgaacaatca
     6481 agaacgggaa aaactaaagc aacgtgtcat tatgcagcta agaatgctga ttcgtggctc
     6541 cacctattat gccaataaac aagcttcatc aacataaaaa aacagacgcc taagcgtctg
     6601 tttttattct ttcgtaatat atgcagtcga tagctttaac cattctagtg ctaattgatg
     6661 atgttcagat aacgcactcc ataacgcatc ttctgacaag atactataat tgcctttagt
     6721 atctaactgt tcatcaaaac tatcatgtaa ttcacgccat tccgggctag cataaaaccg
     6781 acttaagggt tccaaaagtg cagaagctcg tttccattgc tcaatcccct tttccaattc
     6841 tggtaatagc tctacccatt ggttataaca atcctgcatt ttctgaatat ctgctttttt
     6901 cattctttac cgcttacacc ataaatttta gcctcaatat aacataaaaa tccccttccg
     6961 aagaagggga tttcaactca attaggctaa attaccactg gtatccaacg cctacaccgc
     7021 caccaaagtc gcctcggcta ttcgcattac cttgtaattt caagataagc ttaccgttat
     7081 cacttgaacg tgaataacct actgcaagcg cgttttcacc tttaaatgta ccacctgctg
     7141 cagccaccat agatttacct ggaatgtaaa cctgcggtaa cgctgctgct gcatttgaac
     7201 cggcaatacc agcacgggct tcacggttat tacgatggat cttgttattt aagtgattaa
     7261 cagcaccttt taactgacca acattaacag catcggtatc tgcctcacct gctttaacat
     7321 tagtaatacg gttaccacca ttatttaagc catctttcgt taagcttacc gcttttcctg
     7381 cagagccatt attaatggtt acgccattat tgttcaccaa tgtatcacca gtcttgctag
     7441 taaattcagg ggtatctgaa acggctacag ttacatcaga acctttacgt gtcactttga
     7501 tattattacc tgcattcacg ctcactgtac tacctgcttt tactttcgca ggggctttat
     7561 ccccttcaac cgcacctgta ccaactgctt tagaatctac gttccagctt acgttgttta
     7621 cggcatttgc tactgttgtt gcattcacaa agcgtgaacc ggtaccgtta ggatcttgaa
     7681 cattgatttt gccgttatca gcaacggtta aatctgtgat gtttacgttc actttcacct
     7741 caccagcctt atcactggta atagttgtac cattaccatt tacgatattg atagaaccat
     7801 cttcatcagt accaatgtta ttaccattca ctttcgcata gttgttcagc tgtttaccgt
     7861 tgataacatc tgtgcttact gggctaatat cgccatctgc tacattagtg atcttagttg
     7921 gtttaccgtc tttatcaccg actttgatgt tatcaccatc attagtgatt ttcggaccat
     7981 tctcactgaa ctgtacgcta ttgaagctca catcttttgc tgtcgcaaaa gtaaattgat
     8041 taccatcacg tttaacagtt aagttatcac ctgcttcaaa cacaacttct tcgcccgttt
     8101 tcactagctt agtcgcagct tggtcaccgg cagccttatt acctgcagat ttaatattaa
     8161 agcctgcatt attcactgca tctgcaacag tttgagcaat caataacgca tttggctgag
     8221 ttggtgcttt caccgtacca tcttcgttgt tacctaactc actggtattt gcagtgatgt
     8281 taccttcatt atctacttta atagtagtac cgtcagtttt cgcttcaacg ttataagtag
     8341 ttgaaccatc agcattcgtg ctaggtgtga tagttacacc tcggttaccg cccactttcg
     8401 tagtagctgc attagttgcc gcttgtaaat cacctaagtt cactacgttg tttagcttgt
     8461 cgccttcagc atttttaatc gcttctgcac ggtctttacc ctctggggtt gagccatctt
     8521 cgttacgaac actacttgcc acattgccta attgaacagg ggcttcacct gtgaatttag
     8581 ccgtattgct tggcgttgag ctatcagtag gatcttggtt tacaaactca atcgggctat
     8641 ctactttaat cgtattctta ccgtctttgt tttcaaccgt gaccgttgta cctttaccgt
     8701 taataaaatc aaccgtgtca ttatgtacca cagtatcaac cgctttgccg ttaccttgta
     8761 aattccagcc tgcatttaat acatcaccta atgttgctgc attacttaac ttatcagcta
     8821 agttggttgg attattgtct gctggttgaa ccttatcatc tgcagtcaca ggtatcagat
     8881 tagacgcaac attgctaatc gctttattac ctgcattgat accatcttta ttgatagtaa
     8941 ttggattatt tgcaggatct gcttcatcag ttggtacagt tacagtgtta gaggttacgc
     9001 tgctaaactt aacatcatct ttagtcgcat aaatgacttt accatcagct tcttgcttca
     9061 cagttaagtt tttacctgca accatttcaa ctgctttacc cggattaatc agttcatcac
     9121 cacctgaaag cttctctcca ccttctacag ccgacgtttt caacgtaaag cctgagttat
     9181 tgatggcgtt ggtaatatca cctgctgtca ctaatttctt agcatcatca gcggttggtt
     9241 catcaattct accgtcatta tttgcatcat ttaatgccgt agtgttagcc ttgatcgcac
     9301 cggttgtagg atcttttaca attgttacat cgtccgtatt cacagaaaga tcgaacacat
     9361 tcgcgccatc agcatttttt gtcgtgttaa ccgtaactga tttatcagtc gacttcactt
     9421 cttcacgaga tgccatagcg aaagtataaa gctgactacc atttactgca tctttactat
     9481 ctgcatttac gtcaccggca gctacattag ttactttgac tgcattaccg tctttgtcac
     9541 caacattgat attaccacca ttgttagtaa tcttaggacc ttcatttccg aactgtacac
     9601 tatcaaaatt cacatttttt gcagttgaaa cagtaatgtt attcccatca cgggtaatct
     9661 caatgttttt accatttaag aactgaaccg tatcacctgt tttcaccagg ctcttctcgt
     9721 caccgtttgc tttaatgtta aaacctgcat tgttaattgc atctgcaacg gttttagctg
     9781 ttactaatgc atttggttct gtaggctctt ccactttacc atcagggtta tttgttaact
     9841 cgctggtgtt tgccgtgatt tcgcccttat cgtttacctt aatggttgta ccatccgttt
     9901 ttgcttcaac ggtataagtg gttgagccgt catcgttagt tttagatgtt acagtaacac
     9961 ctttatcacc ttccactttg gtggttgctg ctttcattgc tgcttgtaca tcacccacat
    10021 tcacagcatt attcaacgta tcacctgttg cgttgttaat attatccgca atcgctttac
    10081 gctctgcatc tgttggtgct gctttatctg caaatgtacc acccacgccg ctcttcagat
    10141 tagtgattga gcctgttgta tctggtttac cattgctcat atcaatgctg ttgaatgcaa
    10201 cattatcttt cgttgtgtaa gtgaatttac cgtcagcttg ggtaagtgtc atatttttac
    10261 ccgcatcaaa ggtaactgtt tcacttggct tgattagctg tgttttctta cctttggttt
    10321 caccaccatc aacaccatca cctgctgtta cattaaagcc tgaattattg attgcattag
    10381 caatatcacc tgccgtcact aatttcttag catcgtcacc tgtaggctca gtaactttac
    10441 cttcaggaga attatttaat gttgtggtat tcgctttaac ttcgcctgtt gtcggatctt
    10501 tcacgatagt aacgttgtca gtattcactg tcatattacc gtcattatca cgaccgatag
    10561 atttatccgt tgcaacatct accgtatatt cagtattgcc tttatcatct tcctttccgg
    10621 ctttaacagt tacggcttta gaaccagcta ctacagatga ggttttctct gaattattaa
    10681 ttgcatcagc cacattacca cgagttactg taggagcacc tgctttcgct acatctaccg
    10741 tatagattgt ttgaccatta tcacctgttt caggtgcacc aacagttgct aaacctgaac
    10801 ctttcacttc ggttttgtga cgctgctgct tgttttaact gacttacatt caccgcatca
    10861 gtatcagctt cacctgcttt cacgcctgta attttggttg gattaccatc attacctgca
    10921 atattaacat taccatcttt attcgtgatt ttcgggccat tatcaccaaa tgttacgcta
    10981 tcaaatttca catctttcgc tgtttcgtac gtgaatatag tgccaacacg tttaactttt
    11041 aagttatcgc ctgctgcaaa aatcactttt tcgcccggat taatcaactc tgctgcttct
    11101 tgatcaccat cagctttatt gccaccagat tttgcattcc aacctgcttt ttgaatcgct
    11161 tcagaaactg tttttgtagt tgcaacttta tcttcttctg caggtgtagt tactgcacct
    11221 gttgtgttat caaccgtgat agaaccggta tccacatcaa aggttacttt acgccatcag
    11281 cttcttgtgt aacatttgaa actgtacctt taccgtttac aaattgtaca gattcatttg
    11341 gggtcactgt gcctttggtt tcacccgcat tatctttaat attgaaacct tggtttacat
    11401 agttatttaa gttcgtaatt gcatcgccaa cgtttgttgc agtattacca tactgagttc
    11461 cagctgcatt cgttacatta tatttaggct gagtcacaga gccatccgga ttcacaacag
    11521 aaccgccacc aaaattattt gcagttgaat ttgcaaggtt actggttgca ttcattgcca
    11581 tataaagttg tgaaccatta atcgcatcgg ttgatgtagc agaaatctga cccgctgcca
    11641 cattttggat ttgacgctct ttacctgcag agcctacaga aacaacatag ttagtatctt
    11701 tatttacccc ggcaaagcca ttataagtaa taccccctac cgttgcattt tcagttggtg
    11761 ttgctgccct tacgactgat tcgctaccca aagcaacaga actattcaga gtagcttgag
    11821 ctcccatacc taatgctgta gcaaattgag ctgttgcatt agcacgtgta ccaacagcca
    11881 ctgctgattg tgctgaggca attgaatttg cacctacggc taaagaggca tttgcactcg
    11941 catttgctgc aacaccaaag gcagaagctt tttcagccgt tgcattagca ttagaaccta
    12001 ccgctgtagc atcacctgct gtcgcatttg tttggcgacc aagtgcggtt gtaccattag
    12061 ccgttgcatt tgctgaatga ccaactgccg tagcttgaat accttttgaa accgtagcaa
    12121 cacctaaaga taacgctccg ttaccatttg catttgagtt tgtgcctaat gctactgccg
    12181 cttgctctcc acctgttgcc gttgcattcg ggccgatcgc aattgagttt ctgctcttcg
    12241 cgccatcatt agcttgattc tcaggtacgg ttgaattcac agagaaatat tgaatttggc
    12301 ttgcttgcgc aggaatattg acagaaagat cataaataac cgtgccattt gcatttctct
    12361 ctgtactttt tacagttact gtaccatcgt ttgaaacaac agtagcttgg gtatctttcg
    12421 taaagtcatc aatggctttc ttagttaaac caatttcata gttagttgta ttcgcagcag
    12481 ttgcagcttt acctgttaca gtaatgtaat cagggctaga agaatttgcc gtggtgttat
    12541 acgcatttac tgtataaata gtttgcccat ttgcaccggt tgcatttgta atattaacat
    12601 tgctacctgc aacaacttct gtttttaccg cttttaatgc ttcttcaatc gtatttttat
    12661 ttgttccacc aatattagta taggtaacag aaccatcagg ctttactgta gcattaccac
    12721 ctaaaatatt tttagttgaa ttagcaaggt tacctgttgc gttcattgcc atataaagct
    12781 gtgaactatt aattgcatcg gttgatgtag cagaaatctg acccgctgcc acattttgga
    12841 tttggcgttt gatcgtgttt gagccaacag aaacaacatt ccctgctgcc gtagtattag
    12901 ccgcaaagcc ggaataagta ataccattta ccgtcgcaga aggaactgcc acagcgttgg
    12961 aaacggttga attactccct aaagcaaccg aattatttag cgctacatta gcacctgagc
    13021 ctaaggcaat agcaaaaccg gcacttgctt tagcactatc accaattgca accgtagatt
    13081 ggttggaaga tactgcactt acccctaaag ctgtcgcatt aacacctgaa actgtcgcat
    13141 tataaccaat cgctgtacct ctagttgcag tagaagttgc attagcatta gcacttgaac
    13201 ctaaagctaa agaataagta gcatttgcaa cagacttatc ccccattgca cctgaaccca
    13261 ttgcaaatga acgagtgcca ctagcggcag atctccatcc cattactgtt gaacggttac
    13321 cagtagctag agctcctgca ccaaaagctg ttgcacggtt acctgttgca gcagtatccg
    13381 tacctaatgc aatagcattt gctgaagtcg ctctagcccc cataccaata gcaatagaag
    13441 gaactaatgc ccaattctga ccagtgttat tgagactctc tacaccaata ttatagtaat
    13501 gtaagaagta tcttctggat ccactaaatt tgttctatgc gttgcattcg tatctgtacc
    13561 aattgcaata gtagatctgc cagttgagat tgaatccgca ccaatagcaa ttgcgttctc
    13621 cgatgatgca gttgcttgtg ttgaaatata attacttgtt gttgaaccta taagatacaa
    13681 cagccacagc atcattagct cccaacacgg cactagctcc agccaataaa gccaatgttg
    13741 ccttacttgc agaactaaca gatttcgttt tacctttcgc tcgttgtaat tcagatactg
    13801 caacccaact ttgtgttgcc tgattccaaa taactttaaa aatttgattc ataatcgacc
    13861 ctatataaaa ataaaaaaga ttacgtagaa cctatgttca acataatctt caccaatata
    13921 aaaaaccgcc acctatttaa gtgaaataac ttacttatag actcagaaat ttaagtgcgc
    13981 gtattttacg ctatttgtaa tataattcat cttctggagt gtaaatttat tgtaaataat
    14041 acgaggaatc taaccgcttg ttataacgca aaacaccttg acttaaatca agccactacc
    14101 tcattttgag gggagattta tacgcagtga aattttaaaa taagctaagg tttacggata
    14161 atcgttcaac actattgaat tatcactgta gttatcgtta aaaaggtatg cttttatgaa
    14221 ccaattcttc cctctaatct ttagcttaac tctattttcc ggcgtaactt tggcactaga
    14281 aaaagcacct cgaactatgc cgacagaagg aataaataag cccctacttg gacaaattct
    14341 attctttgac aatatgctgt cacacaacgg ttcacaaaac tgctccagct gccataatcc
    14401 ggattatgcc tttattgata accgcactac tccggcaaaa ggggtggttt cacaaggaga
    14461 tactccaacc aaattcggca aacgcaactc cccgaccatg ctttatgcga aatactcgcc
    14521 tgagttccac tttgatgagc aaagtcaaga atatatcggc gggcagttct gggacggacg
    14581 agccaaaaat ctcgccgagc aagccggtat gccgcctatc gacccaaatg aaatgggaat
    14641 gcctaacaaa ttagaagtag ccaaaagatt atataatgtg ccgatgtatc agcgattttt
    14701 agggcaattt tacggtgaaa aggtatggga aagttatgaa agcgtttatg cggcaatgga
    14761 agatgcgatt gcgacattcc aacaagaaaa caaacttctc gctccatttg attcaaaata
    14821 tgatctattt ttaaaaggcg aggtacaatt aactgcattg gaagaaaaag gcagagcttt
    14881 atttttcaat aaagctcaaa ctaattgtag tagttgccac caattacaga gtgctgcaaa
    14941 ccaccaagaa gaaaccttta gcaactatca ctattacaat gtaggcgtac ccggtaaccc
    15001 agacttgata gcggttaacg gcttcgcacc agacttcgtc gatttaggct tatttgaaaa
    15061 cccaaatgtt aaaggtgatg aaacacaaaa aggcaaattc aaaaccccaa cattgcgtaa
    15121 tgttgccgtg actgcacttt atatgcacaa cggtgtgttt aaagaattaa agactgtttt
    15181 gctgttttta gacagctaca ataacccgga gcgaaaaatc aaccctgaaa ccggcacggc
    15241 gtggcaacaa gcagaatttg aacccactat cgcccaccaa aaactcaaag ccaaaccctt
    15301 aagcgatgaa gaaattgatg ccctcgaagc atttttaaaa accttgacgg atgagcggta
    15361 tagaaaataa ggctacaagc ggtaaaattt tggggaaatt ttaccgcttg cgtttaacta
    15421 ccgtgttagc tcactttcta acttttccaa tgcctgcaaa tcttcccaat ctgcctcttt
    15481 ctgttttaaa catttacgct gaatatcaaa tttttcgtat tgctctctga caaagctttc
    15541 catttgttta tgcgtgattg ttccaccatt tcctaataca gaaaagccgt taaactcaat
    15601 caacatatct acatttcttc gccagaaacc taacgtaaga tcttggttgt tcttcacacg
    15661 taattcggca ctttcaagaa agatcattac caagcgattt aaagaatcca gctcattatt
    15721 atctaaatag tttttcgcaa tcacaatatc gcctttacgc acgacttttc ctttccagct
    15781 agttaatccc atattcatct tagtcgcatc agcacgttga caaatcaact cagcggcagt
    15841 gtgtttggtt attgcataaa tgagcttatt ttgcgtttct gcaaaaaaca tttgcgtatt
    15901 tttatctgtt ttgtcgtaat cgctagagag tttgaaaagc tctcgtactt tttgatagaa
    15961 acgcaattca cttgcccgaa tctcacgaat ctgttcaagt aattcatcga aataatctaa
    16021 acgaccctgc ggatttttca aacgctcgct atcaattaaa aaaccttttt cgagataagt
    16081 gcgtaatgtg ctgtttgccc attttctaaa ctgtactgct cttgggctac gaactcgaaa
    16141 ccctatcgcc agaatcattt caagtgaata atgttttact tgataattct tgccatcttg
    16201 agcagttatc aagtaatcct tgataactga attctcatct aactctttgt cttttaagat
    16261 atttttaatg tgttcggata tattttgcac agaggtgtca aaaagttctg caagttgatt
    16321 ttgtgtcagc caagcttcat tttcaaccac taacaaggct acattgcttt tgccgtcttc
    16381 cgtattataa ataattaaat cgttcatatt ttattctgtt tattggtaga gatattagtc
    16441 gctattttca ataaaacatg tataaatatc cagaattgat tttgaatttt tcgatgcaga
    16501 tcacaaaaat agagagttat acaaacaagc ggtaaaattt tgtaaaaaat ttaccgcttg
    16561 taaaggttac ttatcagcta atttggcaag tagtttttga tgaataccgc caaaggctcc
    16621 gttgctcatc actaagatgt gatcggtggg tttggcttct ttgacgacta gatcgactaa
    16681 atcatcaata tttgccgacc aatacgccgg ttgcgataac acattggtaa tctcgctcac
    16741 actccacgca ataatatcag gctggtaaac aaacactgct tcggcatcgt gtaggcttgg
    16801 ggctatttca tctttatgga cacccatttt cattgtattt gaacgaggct ctaacactgc
    16861 caaaatgcgt tgatttttgc cgactttacc acgcagagca tcaatagtcg cttctatcgc
    16921 agttgggtgg tgagcgaaat catcataaac ggtaatgccg tttacctcgc ctttcacttc
    16981 caaacggcgg ttagcattca caaacttgcc taatgcctcg caagcccctt gcacttcaac
    17041 ccctgcgtga tgagcggcgg caatcgccat cagagcatta tgcatattgt gcttgccgat
    17101 aatcgaccaa tgcacctcgc cttgtttaac gcctaagtga aacacctcaa actgtgagca
    17161 atcggcattt aacggtttag caaaccattc gttctgttcg ccaataaatt gaagctggct
    17221 gtaactgccc atttttagtg tatcttgcac gttttgatca gcgattgccg acaaaataca
    17281 gccactttgt ggcatcgtgc gaacaaggtg gtggaactgg cgttgaatcg cttttaaatc
    17341 atcaaaaata tcggcgtggt caaattccat attattgata atcagcgttt tcggggtgta
    17401 atgcacaaat ttagaacgct tatcaaagaa cgcagaatcg tactcgtccg cctcaatcac
    17461 gaagaatgga ctgttaccgg cacgagccga aatgccgaaa ttaccggcaa caccgccaat
    17521 taaaaagccg gtatcaatcc cattttggtc taaaatccac gctaacattc ctgttgtggt
    17581 agttttgccg tgcgtgccgg aaaccgccag cacccatttg tctttaagca aatgatcgtg
    17641 caaccattgc gggccggagg tgtaattgag ttggttgtcc aacacatatt ccacgcacgg
    17701 attaccacgg ctcatcgcat taccaatcac cactaaatca ggagccggtt gcagttgagc
    17761 aacatcgtaa ttcgggataa tttcaatacc gtgagcagct aaaaaggtac tcatcggtgg
    17821 gtaaacatta gtgtctgagc cggtcacttt atagccaagc tcacgggcaa tcattgccac
    17881 accgcccata aaggtgccgc aaatgcctaa aatatggata tgttttttca tctttttata
    17941 tttaaaatgt tagaatcatc ccctctcccc ttgtgggaga gggatagatt ttttcttcgt
    18001 gcagaagaaa aaatcaggga gaggggtaag cggtcatttt taggcttaat attgtaagct
    18061 ccctctctct gaaaaatatt tctgaacgaa atatttttcg gtctcaatac tcgctacgct
    18121 cgctcctgtg gagccgttgg caaagccaac gttcaaatgt aaacatttgt cccacaaggg
    18181 gagaggtgcg ctttagatga ggcgtaccat ccctcgttca catcaattaa tcaatattct
    18241 tcaactcttc ttccgaaagc tgctcttgtt ggctttgctc aacattgcca tctttcactt
    18301 taaattcaag gttttggaaa taagcttcac ggaaggtaac ataagggtct tgagcttgct
    18361 ctaacaacgc atcttgttct aataatttag aacgggtatc taccccttgt accacaaatt
    18421 tagcgataga ccaaggtgac ccaacataag tcagcaccga ataaccggtt tccaccgcac
    18481 taccaatacc ttgacgaggt gtggttgctc cataagccgg catcataata taggagccgg
    18541 aaggcacgcc gtgagcgcct aaactctcac ctaaaccacg ttgtccgtta tttaaacgta
    18601 acccctcatc taaactcgcc cagtcaatta acccacctaa accaaacaca gtgttgatcc
    18661 agaagcggtt gaagtgaacc atcgcttttt taccttctcc ttcaattaag cggtttacaa
    18721 agctcaccgg ctcatcaagg ttattagcaa cattaattaa gcctgtttta accgggcttg
    18781 gtacatactc tttccaacct ttagctaccg gttttaacac ataagggtct aaatagtcgt
    18841 aattgacttt ccacatcgca cggttaaacc cctcaagtgg gtcgtctcgc acgccggttt
    18901 ctgggttaat gcttgatgaa caagcggtta aaaatgccac actgagtagc ataaacatcg
    18961 gtttgaacga cttgagtttc ataaattgaa ccttttgcag agaaatttgt ccattgtagc
    19021 gaagttgata aaaaatacaa aacaaaaagc cgttttgcct ctagaaatct agacgtctag
    19081 attgagatat agttcgcttt tgattacaat acaggcaaca tttaaggagc aaacaaatgg
    19141 cagactggaa cggcgaatat attagccctt atgccgaaca cggcaaaaaa agtgaacaag
    19201 tcaaaaaagt gacggtatcc attccaatta aagtattgga aattttaact aatgagcgaa
    19261 cccgtcgcca aatcaaaaat cttcgtcacg caaccaacag cgaattattg tgcgaggctt
    19321 ttttgcacgc ctttaccggt cagcctttgc cgaatgatga agacttgatg aaagacaggg
    19381 cagatgaaat ccctgaagcg gcaaaacaaa aaatgtgtga gctgggaata gacccggacg
    19441 aatggcaata ttagacacac cttgcaactg cgaggttctt tttatgtttt aaggagactt
    19501 tatgtatcaa caattaacaa cagaagcacg ttgggctgtg ttgcttacgc tgatttatct
    19561 gctcggctgg gtaggttttg cttacttttc accgcaaggc agaggcattt tcggctttcc
    19621 gatttggttt gaactttctt gcattttctt acccgtacta tttagcttag ttgccaccat
    19681 tgtcgtcaaa aaagtgtata aaaatatcga tcttgaggtg aataatgaat agtgaaatga
    19741 ttttacccct gattgcttat ttaatttttg tgtttggcgt agcgttttat gcttaccgta
    19801 aacgccaagg cggaagcttt atttcggaat actatatcgg tgggcgttca atgtcgggct
    19861 ttgtgttagc aatgacaacc gccgccactt atgtgggggc aagttccttt atcggtgggc
    19921 cgggtgctgc ctataaatac ggtttaggct gggtgttact ggcaatgatt caagtgcccg
    19981 cagtgctact ctctcttgga gcattaggca aaaaatttgc cttacttgcc cgcaagcata
    20041 atagtgtcac cattaacgat atgttattag cccgttacca aaacaaattt gtggtttgga
    20101 ttgcaggact agccttatta ctttcgtttt ttgcgatgat gacggtgcaa tttatcggtg
    20161 ccggtcgctt acttgaaacc acactcggaa tcccttacca aacagcggta atgatttttg
    20221 ccatcacagt cgggctttat acctttatcg gcggttttag agcggtggtg ctaaccgata
    20281 ctattcaagg tttagtaatg atagtcggca caagcatttt actcgctggt gtgatttatg
    20341 ccacaggcgg agtggaaaat gcaatgcaca cccttgaagc cattaaccca caacttacca
    20401 ccccttacgg cattgacgaa cgcccgcttg atttcacctt tatgacttca ttttgggtat
    20461 tggtttgctt tggtttaatc ggcttaccgc atattgcggt cagaagtatg gcatgcaaaa
    20521 acagccaagc cttgcaccga gcaatgatta tcggcacggt agtcattgca attctaatgt
    20581 ttggaatgca cctttcaggc gtattaggca gagcggttgt gcctgatctc aaagtgccgg
    20641 atcaagtcat tccaacacta atggtacaag tgctaccacc ggtggttgcc ggtattttcc
    20701 ttgccgcccc aatggcagcg attatgtctt cgattgactc aatgttgatt cagtcgtctt
    20761 caaccttaat taaagatctc tatctggcaa ttaagccgaa tgccattaat aacgagaaaa
    20821 aaatcaaatt gttctccact attactactc ttacttttac ggtattactc gtgtttgccg
    20881 ccctaacccc gcctgagatg ttaatttggc tgaacctact ctctttaggt ggtttagaag
    20941 cgactttcct atgggtaatt gtactagggt tgtattggga aaaggcaaat gccaccggtg
    21001 ctatttactc aatgctggca ggcttaagtt gttatgtatt aatgactacc tttaagattg
    21061 ctatttttag cttccacgcc attgtgcctt cactgctaat tggtttgatt gctttcttaa
    21121 tcggcaaccg atttgctcct aaggtgtaaa ataaaatagg catagtggta tggaagttca
    21181 ctatgcctat tttgcaaaat ttccgctcaa ttcaaccgct tgtagttaat aagtggcatt
    21241 attttctgga ttattctgct tagtcgccat tctaagtaac gtataaccta aaatcgctga
    21301 aaccgcagag ccgagtaaaa tacctaatcg agctagagcg gtaatacttt ccccagcctc
    21361 acttacatca aaggcaagac ttgctaagaa catcgacatt gtaaaaccga taccgcatag
    21421 caccgcaatg gcaaaaattt gcttaaaatt aaccccttgc ggcaggctgg caaggcggaa
    21481 taatactgct aaataactaa aaccgaatac acctaaggtt ttgccgataa ttaagccgag
    21541 cgcaatacct aacggcaatg gtgatgagag ttgctccatt cccatttcta ataagtaaac
    21601 gccggcatta gcgaaggcga agatcggtaa aatcatataa gcacaccaag gggcaagaat
    21661 atgttctagc tcctctgccg gtcgttcccc atgtttacct ttcagcggta cgcagaagcc
    21721 gatgatcacg ccggcaaggg tagcgtgaac ccctgatttt aaaacggaag tccatagcac
    21781 taagcctact accaaataag cacagagtga ggtcacttta aagcggttaa gtagcactaa
    21841 gccgataatc gcaatcgcgg caatagttaa ggcttgcaca ctgatttctt gcgaatagaa
    21901 aatggcgatt accacaatcg ctcctagatc atcaataatc gctaatgcca acaaaaatgc
    21961 ctttaaagcc aatggcacac gagtgcctaa taacgccaca atgcccaccg caaaggcaat
    22021 atcagttgcc attggaattg cccaaccatt atgatattca gggtggtttt gattaatcgc
    22081 ccaataaata atcgctggaa caatcatacc acctactgct gctatcgcag gaaaaatagc
    22141 tttcttataa cttgaaagcg aaccttcaaa caactctttt tttacttcca gccccaccaa
    22201 cgtaaaaaac actgccatta agccatcgtt aacccagagt aatagcggtt tattcaaggc
    22261 aaattcacca aactgtacac taacaatcgt ttgtaaaaac tcaaaataga aatcacttaa
    22321 ggaggtgttg gcgcacacaa ttgctgcaaa agcaaaaacc agaagcaata cgcctcctga
    22381 ggcttcagat tttaaaaatt cttttaattt ttccacttaa ctttctccta ttaatatt
//
LOCUS       DANLUA010000014        22354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLUA010000014 DANLUA010000000
VERSION     DANLUA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1374
                     /locus_tag="PXB44_000463"
     CDS             <1..1374
                     /locus_tag="PXB44_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL5280978.1"
                     /translation="LSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEGA
                     GFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHSP
                     SGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVACA
                     LTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMIL
                     GLLFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLATN
                     STIIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGGV
                     PALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSPL
                     ATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVFG
                     WIAAMTL"
     gene            1472..2275
                     /gene="xthA"
                     /locus_tag="PXB44_000464"
     CDS             1472..2275
                     /gene="xthA"
                     /locus_tag="PXB44_000464"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL5280979.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            2429..3013
                     /gene="lptC"
                     /locus_tag="PXB44_000465"
     CDS             2429..3013
                     /gene="lptC"
                     /locus_tag="PXB44_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL5280980.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            3037..3543
                     /gene="lptA"
                     /locus_tag="PXB44_000466"
     CDS             3037..3543
                     /gene="lptA"
                     /locus_tag="PXB44_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL5280981.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            3555..4280
                     /gene="lptB"
                     /locus_tag="PXB44_000467"
     CDS             3555..4280
                     /gene="lptB"
                     /locus_tag="PXB44_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL5280982.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            4322..4834
                     /locus_tag="PXB44_000468"
     CDS             4322..4834
                     /locus_tag="PXB44_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5280983.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            4847..5710
                     /gene="rapZ"
                     /locus_tag="PXB44_000469"
     CDS             4847..5710
                     /gene="rapZ"
                     /locus_tag="PXB44_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL5280984.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            5793..6977
                     /locus_tag="PXB44_000470"
     CDS             5793..6977
                     /locus_tag="PXB44_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5280985.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            7109..7693
                     /locus_tag="PXB44_000471"
     CDS             7109..7693
                     /locus_tag="PXB44_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL5280986.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            7760..8428
                     /locus_tag="PXB44_000472"
     CDS             7760..8428
                     /locus_tag="PXB44_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL5280987.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKYDPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAP
                     APQQ"
     gene            complement(8509..9444)
                     /gene="ftsX"
                     /locus_tag="PXB44_000473"
     CDS             complement(8509..9444)
                     /gene="ftsX"
                     /locus_tag="PXB44_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL5280988.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(9454..10107)
                     /gene="ftsE"
                     /locus_tag="PXB44_000474"
     CDS             complement(9454..10107)
                     /gene="ftsE"
                     /locus_tag="PXB44_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL5280989.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(10100..11596)
                     /gene="ftsY"
                     /locus_tag="PXB44_000475"
     CDS             complement(10100..11596)
                     /gene="ftsY"
                     /locus_tag="PXB44_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL5280990.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            11675..12265
                     /gene="rsmD"
                     /locus_tag="PXB44_000476"
     CDS             11675..12265
                     /gene="rsmD"
                     /locus_tag="PXB44_000476"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL5280991.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            12349..12663
                     /locus_tag="PXB44_000477"
     CDS             12349..12663
                     /locus_tag="PXB44_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL5280992.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            12676..13188
                     /gene="hpaC"
                     /locus_tag="PXB44_000478"
     CDS             12676..13188
                     /gene="hpaC"
                     /locus_tag="PXB44_000478"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL5280993.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            13306..14178
                     /gene="tehB"
                     /locus_tag="PXB44_000479"
     CDS             13306..14178
                     /gene="tehB"
                     /locus_tag="PXB44_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL5280994.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            14316..15353
                     /gene="purM"
                     /locus_tag="PXB44_000480"
     CDS             14316..15353
                     /gene="purM"
                     /locus_tag="PXB44_000480"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL5280995.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            15431..15709
                     /locus_tag="PXB44_000481"
     CDS             15431..15709
                     /locus_tag="PXB44_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL5280996.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            15852..17279
                     /gene="aspA"
                     /locus_tag="PXB44_000482"
     CDS             15852..17279
                     /gene="aspA"
                     /locus_tag="PXB44_000482"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL5280997.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(17354..19372)
                     /gene="rep"
                     /locus_tag="PXB44_000483"
     CDS             complement(17354..19372)
                     /gene="rep"
                     /locus_tag="PXB44_000483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL5280998.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            19620..20735
                     /gene="potA"
                     /locus_tag="PXB44_000484"
     CDS             19620..20735
                     /gene="potA"
                     /locus_tag="PXB44_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL5280999.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            20722..21585
                     /gene="potB"
                     /locus_tag="PXB44_000485"
     CDS             20722..21585
                     /gene="potB"
                     /locus_tag="PXB44_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL5281000.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            21585..22352
                     /gene="potC"
                     /locus_tag="PXB44_000486"
     CDS             21585..22352
                     /gene="potC"
                     /locus_tag="PXB44_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL5281001.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
BASE COUNT         6746 a         4651 c         4406 g         6551 t
ORIGIN      
        1 ttatcaaacg aagtctttat ttcgattatc gtgctgttaa tcctaagctt gcttagaatt
       61 aatgtagtaa tcgcacttat tatttctgcc cttacttgcg gtctaatcgg taatcttggt
      121 gtagaagggg ctggatttac cgaagcattg gaaaaaacaa taaaaagttt tactggtggt
      181 ttaggtgggg gtgcggaaac tgctatgaac tatgccgttc ttggtgcgtt tgccgtagct
      241 ctatcaaaat caggcgtgac cgatttatta gcctataaag tgatcaaatc ctttggtcac
      301 agcccgtcag gtcgctccgt tacttggttt aaatacattg tattatttgt gttagtggca
      361 tttgcaatgt catctcaaaa cctaattcca attcatattg cctttattcc gattttaatt
      421 ccaccgctat taagcgtaat gaataaattg caaattgacc gccgtgcggt agcttgtgct
      481 ttaacctttg gtttaaccgc cacttatatg ttaattccgg caggtttcgg tcagatcttt
      541 attgaaagca tcttagttaa aaatatcaac caagcaggtg aaccgttcca attagtgact
      601 accacagcgg aaatgaccaa agcgatgtta attcctgttt ccggtatgat tttaggctta
      661 ctgtttgcat tctttgtgtc ataccgtaaa ccgcgtgtgt atattgaaaa tatgcctgaa
      721 ccaaccgcag atgatattga agcccgtgtt gcaaacgtta agcccttcca tatcggcatt
      781 agtattgtgg cgattatcgc gacctgttcg gtgcaattag caacaaattc gactattatc
      841 ggcggcttag tcggtctggt tattttcgcc ttaggcggtg tatttagact gaaagagagt
      901 aacgatgtct tccaagctgg cttacgttta atggcgatga ttggctttgt gatgattgcc
      961 gcctccggtt ttgccggcgt ggtcaattca acaggcggtg tgccggcatt ggtagaaagc
     1021 ctgcgtgatg tgatttcatc aaaagaaatg gcagcattgc taatgttagt cgttggctta
     1081 tttattacga tgggtatcgg ctcttcattc tcgactgtgc cgatcattac ctcaatttat
     1141 gtgccgcttt gtatctcatt tggcttctcg ccactggcga caatggcgat tgtcggtgtt
     1201 gctgctgcac tcggtgatgc aggttctccg gcatcagatt ccacacttgg tccaacctcc
     1261 ggcttgaatg ctgacggtaa acacgaccac atttgggatt ctgttgtacc aacttttatc
     1321 cactttaaca ttccattatt agtgttcggc tggattgcag caatgacact ttaagctata
     1381 atatgcaaaa tttttgctaa aaatgaccgc ttgtagccat tacaagcggt ctattttctt
     1441 ctttattttg caaaccacat taggatcacc aatgaaattt atctctttta atatcaacgg
     1501 attacgagcc agaccacatc agctcgaagc gattatcgaa aaacaccagc ccgatgtact
     1561 cggcttacaa gaaatcaaag ttgccgatga agattttcct tacgatttag tcaatcactt
     1621 aggctaccac gttttccacc acgggcagaa aggtcattac ggcgtggcat tactgactaa
     1681 aaaagagcct atagcggtgc gtaaaggctt cccgacagat gtacttgatg ctcaaaaacg
     1741 aatgattatg gcggatatcg agaccgattt cggcgtgcta accgtattaa acggctattt
     1801 cccacagggg gaaaatcgca gccacgaaac caaattcccg gcaaaacaaa aattctatgc
     1861 tgatttgcag aactacttgg aaagcgaatt aaagccggaa aatccgatta ttattatggg
     1921 cgatatgaac atcagcccga ctgatttgga tatcggcatc ggcgagccaa accgcaaacg
     1981 ctggctgcgt gacggtaaat gttcattctt acccgaagag cgtgaatggc tagatcgctt
     2041 atacagctac ggcttggttg atactttccg tgcaggcaac ccagacgtga acgataagtt
     2101 ctcttggttt gactaccgtt caaaaggttt tgatgataat cgtggcttgc gtattgattt
     2161 aattcttgct tcaaaatcac ttgctgagcg ttgcgtggaa acaggcattg atttagacat
     2221 tcgagcaatg gaaaaaccgt ctgaccactc ccctgtttgg gcaaccttta aataaagttg
     2281 caaaaaaatc tgagttttca accgcttgtc agtttttagc cctacaagcg gttatttttt
     2341 ctcacttttt tgcacttttt acgctgtact atatccctat tcaaatctgt gctacaatca
     2401 aacactattt ttagattatt tttggattat gaacaaacgt ttaaccgtta ttttactgat
     2461 tattgttgcc gtacttggtg gctggtatta cagcttacaa gagaaagaga atacggggct
     2521 tgatcagctt attaaaaagg aaggacagcc tgaatatgtg gggaataaaa tgtccaccac
     2581 ggtctatgat ctaaaaggaa acccacaata tttcgctcaa gctactgaaa ttaaacgcta
     2641 tgagtctact gagcgtactg agttttttaa accttttatt gagctttttg ccaaagagtc
     2701 gacgcaaaaa cagtggaaag tcagtgccga ttatgcagaa attaccaaag aaaaaatgtt
     2761 aaatttaaaa ggtaatatca aacttgttgc attagatcct gcatcacgtt tgcaacaaat
     2821 cactaccgat acgttaactg ttgatttaaa tacacaggat atttttaccg aaagtacggt
     2881 caaatcgata ggttccggtt ttactacaac gggggttgga ttaaaaggta acttaaaaca
     2941 acaagttgcg actttgcaaa aagatgtaaa atcttacatt gaaccgacca ttattcaaga
     3001 aactaaagaa taatttgctt ggaacaagga ataacaatga aattaatgtt taaatctatt
     3061 ttagtgagcg tgttactggg tagcagcttt tccgcttatg ctctaaaagg cgataccgat
     3121 cagccgatta atattgattc aggtagccaa tcgttagata tgactaacaa tatcgtgacc
     3181 tttagcgata atgtcgttat cactcaaggg tcgattaaag tgaccgcagc aaacgtcaaa
     3241 attacccgtc aagaaggtaa aaaagaaact attgacgcta caggctcacc tgtaacattc
     3301 caacaaacct tagataacgg taaaccggta aacggtaaag gcaacagcgt tcattatgat
     3361 ttaaactctg aatttttaac gctgattggc aatgcggaac tcaaacaaca aggtagcttt
     3421 attaaagcca ataaaattac ctatgatgtg aaaaaacaac aacttaaagc taccagtggc
     3481 ggtaaaaccc gagtgaaaac cgtgctaatt ccaaatgaac ttcaagataa caagaaaaaa
     3541 taaggttaaa tgatatgtct gtgctttatg ccgaacattt agcaaaaagc tataaacaac
     3601 gccaagtggt aaaagacgtc agcctgaatg tcaaatcagg tgaaatcgtg ggcttgctcg
     3661 gtccgaacgg cgcaggtaaa accaccactt tctatatggt tgtgggtttg gttcgccacg
     3721 accacggcac aattcgtatt gacgaccaag atatcagcac actaccaatg cacgacagag
     3781 caaaacaagg gattggctat ctaccgcaag aagcatctat tttccgccgt ttaagtgtgt
     3841 acgataactt aatgtcggta ttacaagtgc gtaaaaatat caatgataaa cagcgtaaag
     3901 agcgtgccga agagctgatt acagaattcc atattgagca tattcgcaat agtttaggtc
     3961 aatcactttc aggtggtgaa cgccgccgtg tggaaattgc ccgagcatta gcagcgaacc
     4021 cgaaatttat tctgttagat gaaccttttg ctggggttga cccaatctcg gtaattgata
     4081 ttaaaaaaat tatcgtcaat ttaaaagagc gtggtttagg ggtattgatt accgaccata
     4141 acgtacgtga aactctagat gtatgtgaaa gagcctacat tgtcggtagc ggcgaaatga
     4201 ttgccagcgg cacaccacag caagtgcttg aaaaccctga tgtaaaacgt gtttatttag
     4261 gcgatcaatt caaactctaa aaaatcccct ctttggggat ttcatcttta gatgaaaaat
     4321 tatgacaaaa ctaaccgaat ttctaagccc tgaaaatgtt cgccaaggtg tactgatttc
     4381 aagtaaaaaa cgagcgttag aactcgtggg taaaatcgtg gctgaatcaa tcaaccaaac
     4441 cgaacagcaa tctgaagcac tctgccctat tgaatgcttt ggcaatttat ttaaacgtga
     4501 aaagttaggc acaaccggct taaatcaagg cattgcactt cctcatgcaa aattgcccgc
     4561 ttgcggtgat attgagctga ctaaaccgat tgcggtcttt ttacaactgg aagaagcgat
     4621 tgactacgaa gcccaagaca acaaagaagt ggatttaatt tatgcaatga tgtttccgga
     4681 aaatagctgc gaacagtata aaagttgcct acctgcaatt gcacagaaac ttaccgataa
     4741 atcgttatta aaacagctcc gagcagctga atccgcagaa gatatttggc aaattttggt
     4801 gtatgctgat aatcacctaa aagatgaaga ataatactga ggaaatatgg aattaatcat
     4861 tataagcgga cgctcaggct ctggtaaatc ggtagcatta agagcattag aagatgcagg
     4921 ttattactgc gtggataata tccctttacc tttaattcca accttaacct actaccttac
     4981 tcaagaaaat cgttcggcag tcatcagctt agatattcgt aatttaccga atacgcctaa
     5041 tgcgattgaa gaattgctta cccagcttga gcctttaaat gctaagctga tttttttaga
     5101 ttgcgaacgc aataccttaa tccgccgtta tagcgattcc cgccgaatcc acccgctttc
     5161 cacccaagac gatctctcgt tagaaggtgc attggatttg gaacaaaaat tgcttgagcc
     5221 tttggtgcaa aatgcaaatt atattattga caccagtcat ctttcttcgc acgaattagc
     5281 ggaaaatctt cgccaaatac tgcaaggctc aaccgaaaaa gagcttaaaa ttattttcca
     5341 atcattcggt tttaaatacg gcttacctgc cgattccgat tacgtttttg atgttcgctt
     5401 tttaccgaac ccacattgga atccggagct tcgcccaatg acaggcttag agcaaccggt
     5461 gattgatttc ttagaacgcc aaaccgaagt gcataatttt atttaccaaa cccgcaatta
     5521 tttagaaatg tggctgccga tgttggaaca aaataaccgt agctacctaa ctattgcgat
     5581 tggttgcact ggtggaaaac atcgttccat atttattgcc gagcaactgg caaaatattt
     5641 ccaagcaaaa ggcaaaaatg tgcaagttcg gcataaaagt ttggaaaaac accataaaaa
     5701 aacatcttaa ataagtaggg gctggtcctg tgccagccct catttcaact attttagggc
     5761 gggcacaggg caccgcccct acatttgatt ttatgaaatc cgcagatatt gttattgttg
     5821 gcggcggtat ggttgggctg gctcttgccg gactgctcaa agatgtcgat tgccaaatta
     5881 aaattattga gaaaaacacg cccaaatttg atgccgatac gatttaccac cgtgtaagtg
     5941 cgattaatgc ttcaagccaa aaaatactgg aacagattgg tgcttttcaa cacattccgg
     6001 ctcaacgcct ttctccttac accgaaatga aagtgtggga aaaagacagc tttgctaata
     6061 tccattttga taataatgat aatgaaatca agcaattagg actttcacaa cttggcttta
     6121 ttttggaaaa tgcggtgatt caacacgcat tgtggcaaca ggtttctcaa caagctaatg
     6181 tagaatatat tatcgccgcc ccccaaactc tgggcgtgag cgaaaacggg gcatttttaa
     6241 ccttagataa cggcgaaatg ctgtctgcta aattggttgt cggggcagac ggtgcaaatt
     6301 cgtgggtgcg taaccagatg aaaatgccgc ttaccagtaa agattatcaa cataccgcct
     6361 tagtctgtaa tgtcaaaaca accgaaaagc acggcaaaac cgctcgtcaa atctttgctg
     6421 aagatagcat ttttgctttt ctgccaatgg cagatgagca tctctgctcg attgtttggt
     6481 cgctgccacc aaacaaagcc aatgaattaa aaaatgcaag cgaagccgaa tttaataaag
     6541 ccttaacgat tgccttcgat aatcagcttg gcttatgtga agtgcaaagt ttgcgtgaaa
     6601 tttacccgct tgcagcacgc ttctgccgtg actttgccca acctcgtatt gcgttgattg
     6661 gtgatgccgc tcataccatt cacccattgg cgggacttgg tgtcaattta ggctttgccg
     6721 atgccagaac gctggcaaac gaaatccaaa aacagttggt aaacaaccaa gatattggtg
     6781 aataccgcca tttacgccaa tttgaacgtg agcgtaaagc ccaagcgatg aaattattag
     6841 ccacaatgga aggattaaaa cagctatttg acggcaatca cccgcttaaa aaattattca
     6901 gaggaattgg tttaagccta accaaccagc tgccttttgt gaaaaagcag cttattcagc
     6961 aagctattta cttataaaat gtaaattttt tcgaaataac gtaaagatga acgattttat
     7021 gaactaatac ggagattact tgtctattta ttggtacaat aaccagcctt tattggcttt
     7081 tatttttata ttcttgagga attttttaat gaacaaatta acaaaaatca gtgcaaccgc
     7141 tttagtcgca ttattcttag cagcttgtga taaaccagca gaaaaagcac cggcaacaga
     7201 acctacaaaa acaactgaag ctgcaccaac tacaacagct cctgctgcag aagcagccaa
     7261 acctgctgaa acagctgtag ccgctccaag cgaagctgca aaagcagatt accaaaaatt
     7321 agttgattgg aatacggcac aagaaagcac aatggcaaca gcacaagcag aactacaaaa
     7381 gaaattagct acacaagatc ctaaacaaat tcaagaagga ttaagtgcat ttaataaaaa
     7441 agtagaagaa actgtaaaaa gcttagatgc gattgaagta tctgatgcac aaatcaaaac
     7501 atttaaagaa aaaacaaaat cagttttaac actttcaagt gaagtgattt ctgaacaagt
     7561 aaaaacgatc tctacaccaa atgaccaagc agcattacaa gccgtacaac aaaaagctca
     7621 gtctttaatt gaagctggta atgagttaca aaaattaaac gtagaattac agcaaagatt
     7681 ctcagctaaa taatttacca ttcaatgaaa agctagagta ttttgctcta gctttttttt
     7741 gcaattacta aggattacta tgaacacgct cgctaaaatt agcctatctg tattatttac
     7801 ccttttttta accgcttgcg aacaacctaa ttctaccaaa acacagagct cagcagaatc
     7861 gccagttcaa gtgaaagaag agtcaaaaga agaagttaaa cctgctgata ccggcgctca
     7921 agattataaa atgttacggg aatggcaaga cactcaagaa aaagccttaa atgatgcaat
     7981 taaagcggca acagaaacac ttactgacaa acaaaaagcc gattctacct taatgcaaga
     8041 gacagttaat aacgcattat tagctcaaat tgatcatatt aagataagtg ctgaaaccct
     8101 gaatattcaa aataatgaag tcaaagcact aaaagacaaa accttagaag tcctgacttt
     8161 aggggctcag atgatcgtag aaggtgctaa aatggagaaa aatcctacac cggaagcaca
     8221 taaagcattt ggcgagttac aaacaaaatt gaatcaatta gctgaagaag ggcaacagtt
     8281 agaaaatata ttaagagcta aatatgatcc agctccagct ccagctccag ctccagctcc
     8341 agctccagct ccagctccag ctccagctcc agctccagct ccagctccag ctccagctcc
     8401 agctccagct ccagctccac agcaataata tattgcttat agtaaaaaac caagttccta
     8461 ggaactggtt tttatctgca attatttctc taaaaacaac cgcttgtatt agcctctact
     8521 accaatacgg aaaatatttc gcattgtcgc aatttttgcc gacagccagc caataaacac
     8581 acaaagcacc actaagaaga aggcttcgct gaaatctaat ccgttcagtt caaattgtac
     8641 ggtaaacata tcagtcacat acttcactac accggtgaaa tagctaatcg ttaccgcact
     8701 aaacagcacc gctaacaggc tgccaaacaa gccgtaaatc atacctgtgt ataaaaacgg
     8761 acgggcaata aagtgatcag ttgcccctaa aatttgttgg acttcaatcg ctgctttgct
     8821 attggcaaca tcggttcgca cactgtttcc aatcactaaa aaaactgcca acagcattaa
     8881 taaggtacac accaccgcaa tacgagcaat cagccaagtt agagcagtta atttttccag
     8941 ccagccgtta tctaaacgaa cctcctgcac ccctttaatt tgttgcaaac cgttgcgtag
     9001 ctccaccatc gtatcagcat tattaaaatt tgctttcggt tttaaggtaa cgaccgcagg
     9061 caacgggttt tcatctaaaa tatctaacgc ttcaccaaag cctgaccaag aacgaaattc
     9121 gtctaaactt tgttggcgag aaatatagtt aaatgactca attttatccg tctcaaaagc
     9181 acgaatgcga tcaacaacgg tattcacatc gtgttctgcc aaatttttgt gcagataaac
     9241 ggttaattgt ggctcagggt aaaattgtgt tgctgcctcg tgggtattct tccaaagtaa
     9301 ataactcacg gttggaatcg taagtgagac cgcaatcacc agcaccgtca gaaaagtgcc
     9361 gagcttgcgt tgcattaaat cttgccaaac cgctcttaaa gagtagttgg tttgagcacc
     9421 taatttacta aatgaacgaa tcataatatt tccttaaaat ttaagatgcc cttgctccag
     9481 cactaaacac ggctttggac gctttgaaat aatattggta tcgtgggtgg caatcagcac
     9541 cgttgtgccg gcttgattaa attcctcaaa caaacggaaa atttcaaacg agagcttttc
     9601 atctaagttt ccggttggct catcggctaa taacagcgtt gggcgatgaa caatggccct
     9661 ggcaatatcg acacgttgtt gctcaccgcc ggaaaggtgc aacggcagat agttcgcttt
     9721 atctgccaag ccaacacgct caagggcaat gcgggcttca cgctccacaa tcgcttggtt
     9781 cattcccaaa ataatcaatg gtaatgcgac attatccaaa atggttcggt cggttaataa
     9841 gcgataatct tgatgcacca tcccgatttg gcggcgtaaa aacggcaatt catgatgagc
     9901 aagtcgggta atatcgtgtc cgttaaacaa aatttgcccg ccattggcac gctcaatccc
     9961 cataattaat tttaatagcg tgcttttccc cgcaccggaa tgcccggtaa tatatgccat
    10021 tccaccggca ggtaggtgga aatcaatgcc ctgtaaggct ggctttccgc ccttataaac
    10081 cttgttcaca ttagtgaatt taatcattct gttcgtcctc gtggctaaat aaggcttcaa
    10141 taaattcttc tgcattaaac gggcgtagat cttcaatttt ctcgcccacc ccaataaagc
    10201 ggatcggaat attgaattga tcggcaatcg caaaaatcac gccacctttt gccgtaccgt
    10261 ctaatttagt taaggtgata ccggttaaac ccactgcctc attaaacaat ttcgcttggc
    10321 tgatcgcatt ttgcccagtg ccggcatcaa gcgtaagcat aatttcgtgc ggtgcggttt
    10381 catcatattt tttcatcacc cgcacaattt tcttcaactc gtccatcagg ttatttttgt
    10441 tttgcaaacg ccctgcagta tcagcgatta gcacgtcaat cccttttgcc gcagcggatt
    10501 gcatcgcatc aaaaatcacc gaggcagaat ccgaaccggt gctttgtgcc accaccggaa
    10561 tgttatttcg ctctccccaa acctgtagct gttcaactgc cgccgctcgg aacgtgtctc
    10621 ctgctgctaa cattaccgat ttgccttctt gttggaattt acgggcaagt ttgccaatgg
    10681 tagtggtttt ccccacgcca ttcacaccaa ccattaaaat gacatacggt tttttcgctt
    10741 ccaacactaa cggttgagcc accggtttta gcacttcacc cagttccact ttcaactgtt
    10801 gatacaacaa atctgcatct tttaattgtt gcttggtagc atgttgcgtt aaattgttga
    10861 tgattttttg tgtggtcggc atacctaaat ccgccacaag caactgctct tccagctctt
    10921 caaataactc atcatcaatt ttcttgccac ggaagaaatt acggaagccc gaaccaatat
    10981 tctgcttggt tttaatcaag ccttgaatta aacgactgaa gaaaccgcct tgactcggtt
    11041 tttcttgata ttcatcttgg atagtagatt cttcaactgt gtcttgaaca ttatttacta
    11101 attcagtttc agcaacttct tcagccacag cctcttcgac ttgataagta acttgctctt
    11161 gttcgttgag atctaactgc tcagttgcaa tattttgaag attttcgacc gcttgttcgc
    11221 ctttttgttc ttcaaatgtt tgttcgattt tatcttcaac atcatcaaag aacgctttcg
    11281 cctcttcaac tttttcttca acaaactctt tgaaatcttc gactttttgt tcaatggtat
    11341 cttctacttg ctcagcaaat tgctcaattt tttcttcttt ttcctcaaaa tagtcttcta
    11401 actgatctac cttttcttca attttatctt ccagctcatc taatttttca gaaaacgact
    11461 tctcagtttc cggttcaaca ataacaggct gttccggttg aatactctct tgttcttgtt
    11521 gaatctcttt ctgttcagct tcttctttct taccaaatcc aagccaagac caaaaccctt
    11581 ttttcttctc tgacatattt ttctctcatt tctcgtaaaa atagactaaa cttacatagt
    11641 ctatcaaaaa atcccttatt ttcctttagt gagtatgaaa aaaaatcgaa attcccccca
    11701 atctgttcag caaatgggcg aagtacgggt tatcgccgga ctgtggcgag gcagaaaatt
    11761 acctgtacta aatgccgagg ggcttcgccc gacaaccgat agagtaaaag aaacgctgtt
    11821 taactggtta atgcacgaca ttgctcactc acgctgtctt gattgctttg caggcagtgg
    11881 ctctctgggc attgaagccc tttcccgcca agcacaagcg gtcgtttttt tggaaaaatt
    11941 tgcaaatgct gccaaccagt taaagaaaaa tctccaagca ttaaaatccg aacaaggtag
    12001 cgttattcac actgacaccc tcgcctattt agcacaaaaa aataacggtg agccatttaa
    12061 catcgtattt atcgatccgc cttttcatca agggtttgta cctcaagtgc taaacttgct
    12121 ggcagaaaat aactggctcg ccccgaatgc gattttgtat gtggaaaccg agaaaaatca
    12181 cccgccatta gagctaccgc aaggctggca aattttaaag gaaaaagcag ccggaatggt
    12241 ggtaagccgt ctgattcagc tataatcttt ttgtttttta taactaaaaa atataaaata
    12301 aaacttatct atctaacatt ttattaacac tcaaattaga ataacgaaat gaaaaaatta
    12361 acccctgatg aagccattga tattgcctac gatattttct tagaaatggc aggcgaaaat
    12421 ttagatcctg ccgatatttt actgttcaac ctccaatttg aagagcgtgg tgcagtggaa
    12481 atggtcgaaa cttctgaaga ctgggatcaa gaaatcggga ctttaattga ccctgatgcc
    12541 tttgccgaag tttgggttgg cttagtcaat gaacaagatg aaatggacga tgtattcgcc
    12601 cgtttcttaa tttcccacga tgaagaaaac cgtgaatatc acgttgtttg gaaagaaaaa
    12661 taaaaaggag aaaaaatggc taatatcgac actaatcaac caatagaagc ctcgcttaaa
    12721 tttcgtgaag caatggctca cttatcttct gcggtgagca ttgtgacaac caatggcgta
    12781 gccggcaaag cagggctgac ggtttcgtct gtttgttccg ttaccgattc cccaccaacg
    12841 ctcttatttt gcattaataa aaacagccac gttcacgata ttatccaaca aaacggcaaa
    12901 gtatgcgtta atgtgttaag ccacgaacaa gaagacttag caaaacattt tgccgccatt
    12961 ttagaaagta caatggaaga acgctttggt tgggatattt ggaacaacgg ctttgacgat
    13021 cagcctgtgt tacgcagtgc tatttctgct ttacaaggca atattgttga tacgcacgca
    13081 gtgggtacgc atactatttt tgtggtagaa gtgagccata ttgacgtaga gccgaaccac
    13141 agtttagttt attttgcccg ccaatttaga agcgtgaaca ttgactaaaa tatatttaac
    13201 agaaatttga tcaaatccga cttttttagt cggatttttt atttcctgtt tttaaaacag
    13261 tgctaaaatt ttgccgttta tgtttgactg acaaaaggaa acaaaatgga aaatttaatt
    13321 tgctacaaac aaatgccggt gtggacaaaa gcaaccttac cacaaatgtt ccaagaaaag
    13381 cacaacacca aagtcggaac ttgggcaaaa ctcaccattc tcaaaggttc tctcaaatat
    13441 tatgaattaa ccgaagaggg agaaattatc aaggaagata tttttgatgc aaaccaccaa
    13501 cgcccaatgg ttgagcctca actttggcat aaggttgaag ctctttctga cgatttagaa
    13561 tgccagttgg ctttctattg caagccggaa gattattttg ctaaaaaata taatctgacc
    13621 gctacccatt cagaagtgct aaatgccgtc aattatacca aaggtggcaa agcgttagat
    13681 ttaggttgcg ggcgaggacg caatgcactt tatctgaatt tactgggttt tgatgtcact
    13741 gctgttgatc ataatcaaga aagcattgat tttctcaact atatgatcga aaaagaacaa
    13801 gctcaaaaca ttactaccgc tgtttatgat attaacgagg caactatcgg ctcgcaaaac
    13861 ggtgaatatg acctgattat ttccactgtt gtaatgatgt tcttaaaccg tgaacgcatt
    13921 cctgccatta ttgagaatat gcagaaaaac actaaagtcg gcggttataa cttaatcgta
    13981 tgtgcaatga gtaccgatga ctacccttgc ccgatgccgt tctcctttac cttcaaagaa
    14041 ggcgagctcg ccgattatta tcaaggttgg gaattagtca aatataacga agatttagga
    14101 catttacaca aaactgatga aaacggcaac cgcattcaac tacgttttgc gacaatgttg
    14161 gcgaaaaaaa tcagctaaac ttaaccgctt gcagggggaa ttagcttccc cctaattcat
    14221 caaatccatt ctatttattt tcactttttc attccgcaaa cgtttgcttg tgctataatg
    14281 gcttcgtttt ttagtcattt actgttgagg ttaaagtgag caacacacaa ctaagttaca
    14341 aagatgccgg cgttgatatt cacgcaggaa acgagttagt cgaacgaatc aagcctgatg
    14401 ttaaacgcac ccgtcgccct gaagtaatgg gcggattagg tggttttggt gctttatgtg
    14461 ctttaccgac aaaatataaa gagccgattt tagtttccgg taccgatggc gtcggcacaa
    14521 aattacgctt agccattgac ttaaacaaac acgatactat cgggcaagat ttggtagcga
    14581 tgtgtgtgaa tgaccttgtc gtgcaaggtg ctgaaccgct tttcttctta gattattacg
    14641 ccaccggtaa attagaggtt gatgtagcca ccaatgtcgt caaaggcatt gcagacggtt
    14701 gcgtgatttc aggttgtgct ttagtgggag gtgaaacggc tgaaatgccg ggaatgtacc
    14761 acgaaggcga ttacgattta gccggattct gtgtcggtgt ggtagaaaaa tctaaaatca
    14821 ttgacggttc aacggtgaaa accggtgatg ccttaattgc attagcgtcc agtggcccgc
    14881 attccaacgg ctattcgtta attcgtaaag tattggaagt cagccgatcc aatcctgcaa
    14941 ccgatttatt ggaagacaaa cccttaagcg aacacttatt agccccaact aaaatttatg
    15001 taaaatcaat cctaaaatta gttgaagaag ttgatgttca cgctatcgct cacttaaccg
    15061 gtggcggttt ctgggaaaat attccacgcg tattgccgga atctgccaaa gcggtgattg
    15121 atgaatcgag ctggcaatgg ccggcgatat ttagctggtt acaggaaaaa ggcaatatca
    15181 gccgttatga aatgtaccgt actttcaact gcggtgtcgg aatggtgata gccttacctg
    15241 agagagatgt cgaaaaagcc cttactcttc ttaaagagag tggtgaaaat gcgtggctta
    15301 tcggtaaaat tgaagactta ggcaccggta gcgagcaagt tgaaatccgc taatttgcaa
    15361 aataaatgct aaaattgacc gcttgtagta actgatctgc aaacggttaa atttttattg
    15421 aggaatgcaa atgcaactat tttggcctat ctactttgtc ttaatgaacc tagctgcctt
    15481 atatttaatg tatagtgaca aacaaaaagc gatcaaaaaa gaatggcgag tgccagaaat
    15541 tactctcctc tctttttgcc tcttaggtgg ggctatcggt gtttacctag gtatgaaata
    15601 ctttcgccat aaaacccaac attggtattt ccacgtcgct gttgtgcttt ctacttttgc
    15661 ttggctgatt ggcttgcctt acttttattt agctttatca tcatattaat ttatcattac
    15721 tttttttaat ccgaaagccc aatttatatc ctagtccgct cacaaaaaat ttgatttaga
    15781 tcgcattatt tgtctattaa aatcgttatt atctaccaaa atcgattgtc atttatttta
    15841 aggagatgaa tatgtcagat gtgcgtattg aacacgattt acttggtgaa agagaagtac
    15901 ctaatagtgc ttattggggc attcatacat taagagcgat tgagaatttt aatatctcaa
    15961 atgtcaccat ttctgatgta cctgaatttg tgcgtggcat ggtaatggta aaaaaagcta
    16021 ctgcgttggc aaatggtgaa ttgggtgcaa tcccgaaaaa aattgccaaa gctattgtgg
    16081 cagcctgtga tgaagtatta gtgaaaggac gctgtctcga tcaattccct tcagatgttt
    16141 atcaaggtgg agccggcaca tcggtgaata tgaataccaa tgaagtgatt gccaaccttg
    16201 cattagaaat tttgggcgag gcaaaaggta aatatgatat tgtcaatcca atggatcacg
    16261 ttaatgcaag ccaatccacc aatgatgcct atccaaccgg tttccgcatc gcggtttata
    16321 acagtatcac taaattaatc aataaaatca cttacctaca aactgcattt gaaaataaag
    16381 ccactgagtt ttccgatgta ctaaaaatgg gaagaacgca gttacaagat gccgtgccaa
    16441 tgacgctcgg tcaggaattt aaagcttttg ccgtattatt agatgaagag gttcgtaacc
    16501 ttaaacggac ggcagaattg cttcttgaag tgaatttagg cgcaaccgct atcggtactg
    16561 gtctgaatac gccagagggt tactctactc ttgcagttaa atatttagcc gaagtcacgc
    16621 aactgccttg ctcactgtct gaaaatctca ttgaggcaac atctgactgt ggtgcttatg
    16681 taatggttca cggtgcatta aaacgcaccg ccgtcaaact ttctaaagtg tgtaatgact
    16741 tacgattact ttcatcaggc ccacgtgccg gcttaaatga aattaatctt ccggaattac
    16801 aagccggctc atctattatg ccggcaaaag taaaccctgt tgtacctgaa gtcgtaaacc
    16861 aagtgtgctt taaagtgatt ggtaacgaca ccaccgttac ttttgcagca gaggccggtc
    16921 aattacagct taacgtaatg gagccggtta tcgggcaagc aatgtttgaa tctatcgagc
    16981 tgctttccaa tgcatgtatt aatctacgtg ataaatgtat tgacggtatt accgctaacc
    17041 gtgacatctg ccaaaactat gtgttcagct ctattggtat tgtgacctac ctcaatccat
    17101 ttatcggcca ccataatggt gatattgtcg gcaaaatttg tgcaaccacc ggcaaaagtg
    17161 tacgagaagt tgtgcttgag cgaggcttac tcaccgaaga acaactcgat gatattctct
    17221 cgattgagaa cttaatgaat ccgacttata aagccaaacg ctttaccgaa gaggaataat
    17281 cattgtttac aagcggtctg attctggcaa aaaattgcaa atttttacca aaatcagacc
    17341 gcttgttatc ccattatttc ttatttgctt tcagcatcgc cattaaattt gcaatatttg
    17401 cagaagattt tgcctctttt tcctgttcgg tcattcttgg cttttcactt tcccactcca
    17461 aatcatcttg tggcaattcc agtaaaaacc ggctcggctc aggtttgatg atttcgccat
    17521 attggcgacg ttctttacac agtgaaaacg tgagcgtttg ttgggctcgg gtaatgccga
    17581 cataagccaa acgccgctct tcttctacat tgtcctcatc aaggctggtt tggtgtggca
    17641 gaatcccctc ttccataccg attaagaaaa cgtgcggaaa ttccaaccct ttcgaggcgt
    17701 gaagagtcat tagttgaact tgatcggctt catcgtcctc ttcgccacgt tcgagcatat
    17761 ctcgcaaggt taaacggttt accacttgca ccaaattcat cggaggctct atttcatcgc
    17821 cctctaacat cgcttgcacc cattcaaaca aggtctgcac attacggctt tgcatttccg
    17881 ctacttttgg gttcgccgcc atttcataca aataggcttc atactgaatt ttcgccaaca
    17941 gatcttttac tgcttcaatc ggatcggatc tatcggcttg atcggcaatt tccacaatcc
    18001 agcgggcaaa atcctgtaag gcttggtaag gctttggcgt gaggcgttgt atgagttcaa
    18061 aatcaaaaat cgcctcaaac aaactgacct gcttttcatt agcaagtatg cctaattttt
    18121 ctagcgttgc cgccccaatt tcccgtttcg gcgtattcac aatacgcaag aaagctgcat
    18181 catcgtcttg attcactacc aaccgcagat acgccatcat atctttgatt tccgctcgag
    18241 caaaaaatga cgtaccaccc gaaattttat acggaatgcg gttttgcatt agcaattttt
    18301 ccaacaagcg ggattgatgg ttgcctcgat acaaaattgc ataatctttg tatttggtct
    18361 ttttcgagaa acgatgggca atcagctcgc ccacaatgcg ttccgcttcg tgttcctcat
    18421 tttttgcctc aatgacctgt aatttttccc cttgccccag ttctgaaaac agccgcttgc
    18481 taaacaagtg atcgttatta tcaatcagaa tattggcaca atgcagaatc cgctggctgg
    18541 aacggtaatt ttgctcaagt ttaatcactt gcaaatcaaa atcttcccgt aaccgttgca
    18601 tattttcagg cttcgcccca cgccaagagt aaattgattg gtcatcatcg cccacgaccg
    18661 taaagtttgg ctcgttgccg acaatcagct taattaactc atattggctg gtgttggtgt
    18721 cttgatattc atcaactaac aaatagcgaa ttttctgttg ccaacgagat tttgcttcgg
    18781 gaaattggcg gaacagcaac accggcaaca taatcaaatc atcaaaatcc aacgcattat
    18841 aagccttaag ttgattctgg tagagctgat aaaaatgtga aaacactcgc tcacgttcat
    18901 cacgcacacg agtaagtacc ctttcaggcg aaagcaaatc gtttttccag ttagaaatag
    18961 tggcaattaa ggctttcagc agatccttat cttccgtcac attttcaggc aataaatgtt
    19021 tcagcaaagc aatttgatcc tgctcatcaa acaaggtcat acccgattta aaaccgagca
    19081 atttatattc gcgttttaaa atttcaaacc caagtgtgtg aaaagtcgaa atggttaaac
    19141 ctttgctgtt ttctttgccg atagaatgat ccacccgttc acgcatttcc cgagcagctt
    19201 tgttggtaaa ggttactgct gcaatctgct taggcgaata gccacaatgt gcaattaaat
    19261 gtgcgatttt attaataatc acccgagtct tacccgagcc ggcaccagcc agcacaaggc
    19321 aagccccttt ggtatattct actgcttgtt gttgttgaga attgagtttc attgaatcta
    19381 ttcaaaagta attttgacaa ctattttaac ggaaagaaaa caagcggtcg aattttcagg
    19441 aaaatttgca aaaaactttg aaaatccgac cgcttgttca tcagagaaaa atccccaaaa
    19501 cgcaaatttt tcctttcaat cttgcccctt tagctatatc attataagtt gattttttag
    19561 gggcaaatgc ctgattttat aactcctttt ctttttaatt taagggaaaa aaacagtaga
    19621 tggaaaaagt agaacagaaa gctatcattg agcttaaaaa tgtaacaaaa agctacggta
    19681 gtaaaacgat tcttaaaaat ttaaatttaa ccattaacga tggtgaattt ttaacaattt
    19741 taggcccttc cggttgtggt aaaacaactg cgttacgttt aattgccggt tttgaagatt
    19801 taactgaagg ttcaattatt cttgatgggc aagatgtttc taacgttccg gcggagcaac
    19861 gcccggtaaa caccgtattc caaagctatg cccttttccc acatatgacg atttttgaaa
    19921 acgtggcttt cgggctgcgt atgcaaaaag tgccaaatgc tgaaattgag ccacgtgtga
    19981 tggaagcatt acgaatggtg cgtttagaag accgcgctca acaaaaaccg gctcagcttt
    20041 ccggtggtca gcaacaacgt attgcaattg cccgtgcggt ggtaaataaa ccgaaagtat
    20101 tgttgttaga cgaatcgctt tctgcgttag attataaatt gcgtaaagag atgcaaaatg
    20161 aattaaaagc attgcaacgt caattaggca ttacctttat ttttgtgacc cacgatcagg
    20221 aagaagcctt aaccatgtct gatcgtatta tcgtaatgaa cgctggtaaa atcgctcaag
    20281 acggtacacc acgtgagatt tacgaagagc caagtaactt atttgtagct cgtttcatcg
    20341 gcgaaattaa cgtttttgat gcaactgtaa ttgagcgtgt tgatgcaact aatgtaaaag
    20401 caaacgtaga aggtcgcatt tgcaatatca aagtggaaga tttagaggtt cagccgaacc
    20461 aaaaattaaa agtattgtta cgccctgaag atattgtgat tgaggagtta gacgaaaacg
    20521 aaagctcgaa agcaattatc ggtcatatta tcgaccgtaa ctacaaaggt atgacattag
    20581 agtctagcgt gagattagat cataacggta tgaatgtatt ggttagcgag ttcttcaatg
    20641 aagacgatcc gaacattgac cacgaagtgg gacaaaaagt tgctcttact tggtatgaag
    20701 gctgggaggt tgtgttaaac gatgaaggcg aataacaaat tccaaaatgg tgcggtcgca
    20761 actattttcg gttggttatt acttttcgta ttagtgccga acgtactcgt tttaatcatc
    20821 agcttcttaa cagaaaaccg tcaaagtcag tattttgtgg actttgcatt tagttttgat
    20881 gcttataaat cactgtttaa tgacacctat gcagcagtat tgtggaactc gctatatatg
    20941 gcgggcattg ccaccttttt ctgtttgatc atcggctatc cctttgcatt ttttattgcc
    21001 aaaatgccgg aaagaattcg tccgttttta ctgtttttag tagttctacc gttttggact
    21061 aactcactga ttcgtattta tggcattaag attttcttag gcgtaaaagg ctttttaaat
    21121 gaaagcttga tggctttagg tataatcagc gagccaatgc gtttattaaa ctcagaaatt
    21181 gcgattatta ttggtttagt ttacattcta ttacctttta tgattctacc gctttattca
    21241 tcaatcgaga aaattgataa ccgcttacta gaagctgcaa aagacttagg tgcaaacgcc
    21301 ttccagcgtt ttattaaagt gactattcca ttaacaatgc cgggcattgt cgcaggttgt
    21361 ttattagtat tacttccggc aatgggtatg ttctatgtgg cagatttatt aggcggcggt
    21421 aaaacaccgt tagtaggtaa cattattaag agcttgttct taaataccaa ccaatttgca
    21481 ttaggttcag cagtaagtat tgcattaact atcttaatgg cattaatgct ttatgtttac
    21541 tatcgtgcaa acaaattatt aaataaaaag gtggagcttg aataatgaaa aagatgttta
    21601 gaaacctttt tatgttggtt gtttttgcat atctctatat tccaattatc attttagtga
    21661 tcaactcctt taatgaagac cgctttggat taaattggaa aggctttact tggaattggt
    21721 atgagcgttt attcagtaat gatacgcttg tacaagcaac attgaactca ttaactattg
    21781 cgttctttgc ggcaacattc tcaaccattt taggggcatt aacctctatt gcgctttacc
    21841 gttaccgttt ccgtggtaaa aagctagtgg gcggaatgtt attcgtggta atgatgtcgc
    21901 ctgatatcgt aatggcggta tctatgctcg tattatttat gatcttaggt atcaagctag
    21961 gctttatttc gctattaatt gcacacatta ctttctgttt gccttatgtg gtaattgcaa
    22021 tttcctcacg cctcagcgat tttgacggca aaatgctaga agctgcaaaa gacctaggtg
    22081 caagtgaagt gactattttg cgtaaaatta ttttaccact ggcattacct tcgattattt
    22141 caggctggtt gttaagcttt acgatttcgt tagatgacgt tattgtttca tctttcgtga
    22201 caagcccaag ctacgaagtt ttaccactga aaattttctc attggttaaa acaggtgtaa
    22261 cacctgaagt gaatgcacta gcaactatta tgattatttt ctcattaaca ttagtggtat
    22321 taagccagtt agtagtgaag aaaaaagtct aaaa
//
LOCUS       DANLUA010000015        22136 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLUA010000015 DANLUA010000000
VERSION     DANLUA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            152..1174
                     /locus_tag="PXB44_000487"
     CDS             152..1174
                     /locus_tag="PXB44_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5281002.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPNKLV
                     IRDSAA"
     gene            1287..1700
                     /gene="secE"
                     /locus_tag="PXB44_000488"
     CDS             1287..1700
                     /gene="secE"
                     /locus_tag="PXB44_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL5281003.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            1702..2265
                     /gene="nusG"
                     /locus_tag="PXB44_000489"
     CDS             1702..2265
                     /gene="nusG"
                     /locus_tag="PXB44_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL5281004.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            2498..2926
                     /gene="rplK"
                     /locus_tag="PXB44_000490"
     CDS             2498..2926
                     /gene="rplK"
                     /locus_tag="PXB44_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL5281005.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            2931..3620
                     /gene="rplA"
                     /locus_tag="PXB44_000491"
     CDS             2931..3620
                     /gene="rplA"
                     /locus_tag="PXB44_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL5281006.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            3682..4101
                     /locus_tag="PXB44_000492"
     CDS             3682..4101
                     /locus_tag="PXB44_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281007.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            4188..5410
                     /locus_tag="PXB44_000493"
                     /pseudo
     CDS             4188..5410
                     /locus_tag="PXB44_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            5403..5807
                     /locus_tag="PXB44_000494"
     CDS             5403..5807
                     /locus_tag="PXB44_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL5281008.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            5917..7350
                     /locus_tag="PXB44_000495"
     CDS             5917..7350
                     /locus_tag="PXB44_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5281009.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            7364..8092
                     /gene="fabG"
                     /locus_tag="PXB44_000496"
     CDS             7364..8092
                     /gene="fabG"
                     /locus_tag="PXB44_000496"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5281010.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            8155..9300
                     /locus_tag="PXB44_000497"
     CDS             8155..9300
                     /locus_tag="PXB44_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL5281011.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            9314..10219
                     /locus_tag="PXB44_000498"
                     /pseudo
     CDS             9314..10219
                     /locus_tag="PXB44_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            10242..11384
                     /locus_tag="PXB44_000499"
     CDS             10242..11384
                     /locus_tag="PXB44_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL5281012.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            11433..11648
                     /locus_tag="PXB44_000500"
     CDS             11433..11648
                     /locus_tag="PXB44_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5281013.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            11929..12420
                     /gene="rplJ"
                     /locus_tag="PXB44_000501"
     CDS             11929..12420
                     /gene="rplJ"
                     /locus_tag="PXB44_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL5281014.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            12479..12847
                     /gene="rplL"
                     /locus_tag="PXB44_000502"
     CDS             12479..12847
                     /gene="rplL"
                     /locus_tag="PXB44_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL5281015.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(12954..13784)
                     /locus_tag="PXB44_000503"
     CDS             complement(12954..13784)
                     /locus_tag="PXB44_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5281016.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            14010..15551
                     /locus_tag="PXB44_000504"
     CDS             14010..15551
                     /locus_tag="PXB44_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL5281017.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            15554..16342
                     /locus_tag="PXB44_000505"
     CDS             15554..16342
                     /locus_tag="PXB44_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL5281018.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            16355..17612
                     /locus_tag="PXB44_000506"
                     /pseudo
     CDS             16355..17612
                     /locus_tag="PXB44_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(17691..19700)
                     /locus_tag="PXB44_000507"
     CDS             complement(17691..19700)
                     /locus_tag="PXB44_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5281019.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(19710..21047)
                     /gene="rimO"
                     /locus_tag="PXB44_000508"
     CDS             complement(19710..21047)
                     /gene="rimO"
                     /locus_tag="PXB44_000508"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL5281020.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(21202..21894)
                     /gene="mtnN"
                     /locus_tag="PXB44_000509"
     CDS             complement(21202..21894)
                     /gene="mtnN"
                     /locus_tag="PXB44_000509"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL5281021.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(21952..>22136)
                     /locus_tag="PXB44_000510"
     CDS             complement(21952..>22136)
                     /locus_tag="PXB44_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL5281022.1"
                     /translation="LETKVAFQELIIEELNQALIEQQFAIDKLQTQIRHIAEKLKGAQ
                     LSQVASQAEETPPPHY"
BASE COUNT         6501 a         4476 c         4935 g         6224 t
ORIGIN      
        1 ctatcaaaca gaaaatatta aaaatgtgat agcgttaaca tttttagtat ttaattagat
       61 aaaaaataat cagttggcat tttgctctgt tctttctaaa taggaaaatt aaggatttag
      121 ccctataata tggaacaatt aggaaaatag tatggcaaat ggaacaagta gaaatcgaag
      181 atcttcgggc agagtcactt tagctgatgt agccaaagtt gttggtgttg ggcagatgac
      241 ggtatctcgt gctttgcgta caccagaaat ggtgtcggag ccactacgta aacggattga
      301 agaggcaatc attgaactag gatatattcc gcatttttct gctcggcaat tagcttctgc
      361 agaatcacat aatttggtca ttgtgacgac gtcgattagc tcaacggaaa ataccttaat
      421 tttatcggca ttacgcaaaa gcgtggcgga ttccgcattg caaatggtga ttttgatcgc
      481 tgatcaagaa tcttggtttc aagaactgat taatcatgct cctcaagcga tagtgttttt
      541 gaatttagat tgccctccta atgtccgtga gtggattaaa aacagcaata tccctaccgt
      601 agaaataggt gcgataaaaa aagaaccgat tcatctcagt gttggaattg atagccacga
      661 agcaatggag aaaatgatcc attttttggt gaaaaaaggc tatcgagaga ttggattgtt
      721 atcggcgaag caagatttat ccatcttcaa acaatattta gaaagttggc atacgacttt
      781 atttgcacat catatttctc ctttaaaaat ccaccacgta gtagaggata tttcgttttc
      841 ggcaggagca actttgttga ctgaagccct tctaaattgg gaagcaatag atgcattggt
      901 ttttttatcc gatgaattgg cttgcggagc attatatgaa gcattacgcc gacatatgag
      961 cattccaaaa gatcttgcga ttgccggttt aggtggttta gaagtgggga gtatttccta
     1021 ccctaaattg acaaccattg cgatccctta cgagcaaatg ggaaaaatag caggtaaaca
     1081 actacttact cttttgagtg gtaaaacctt atcagcagaa gagatgattg ttaaattacc
     1141 gaataagcta gtgattcgtg atagcgccgc ataatattga ttttgcataa aaatctataa
     1201 aagatcgtgg caagtcttgt agaaaatgat agaatttaac gctttaaaat aatttctgat
     1261 ttttttattc attttttagg ttatatatgg ctactgagat taaaacaaaa acccccgagc
     1321 aagtcgaaca aaaagggggg aaaagcaaag gtgcaaatac agcattatgg ctggttgcga
     1381 ttgtattttt agcagttgct gcggtaggta atgcctattt ttcatctcat ttttcattaa
     1441 ttgttcgagt gcttttattg gtggtgttgc tagttggtgc tgttgcacta gcggcaatta
     1501 caaatcaggg gcaaaaggca attcatttta ttaaagagtc tcgaaccgaa ctacgcaaaa
     1561 ttatctggcc gacacgccct gaagcaacac aaaccacctt gattgtattg gcaatgtgcg
     1621 tagtagtttc cttggtttta tggggtattg actcaatcat cgtcgcatta attacatttg
     1681 taacaagttt aaggttctaa aatgagtgaa gtagaagtga aagaaaatgg ctcaaaacgc
     1741 tggtatgtat tacaagcgtt ttcaggcttt gaaaaccgtg tggcagtaac attgcgtgaa
     1801 tacattaagc ttcacaaaat ggaagagcag tttggtgaag tattagtgcc aaccgaagaa
     1861 gtcgttgaaa atgtgggcgg taaacgccgt aaaaccgagc gtaaattctt cccaggttat
     1921 gtattggttg aaatggaaat gaatgacgat acttggcatt tagtgaaaag tgtgcctcgt
     1981 gtaatgggct ttattggtgg tacggcagat cgccccgcac caattaccaa acgtgaagca
     2041 gaccgcattc taaaccgtgt acaagaaaca gcggaaaaac cacgccacag aaaagaattc
     2101 caaccgggcg aaagcatttt ggttacagaa ggtccgtttg cagatttctc cggtacggtc
     2161 gaagaagtgg attacgaaaa aggtcgctta aaagtatcgg tgtcgatttt tggtcgtgcg
     2221 acaccggtag aattagaatt cagtcaagtg gaaaagcaga gctaatttca attaatttca
     2281 cacacaagcg gttatttttc tcaatttttt gcaaaattca gcggaaaaaa gaccgcttgt
     2341 aacaagattt attcaacaaa gcgaaagttt tgcttgaaaa atagtcgcaa attctttaaa
     2401 atttgcgacc tattacgagc cagcctgctg actcgtattt tttatgtaac aggggagctg
     2461 attttcagcg ttatcaccca tttagaggaa aattaaaatg gcaaaaaaag tccaagccta
     2521 cgttaaactg caagttgcag cgggtatggc taacccatca ccaccggttg gtcctgcatt
     2581 aggtcaacaa ggtgttaaca ttatggaatt ctgtaaagca ttcaacgctc gtactgagag
     2641 cttagagaaa ggtttaccga ttccggttgt tatcacagtt tacgctgata aatctttcac
     2701 attcgttacc aaaactccac cggcggcagt gctactgaaa aaagcggtag gtatcaaatc
     2761 tggttctggt aaaccgaaca aagataaagt gggtacagta actcaagagc aaatccgtca
     2821 aatcgctgaa actaaagcag cagatatgac tggtgcgact atcgaaacca aaatgaaatc
     2881 aatcgctggt acagctcgct caatgggctt aatcgtagag gaataataca atggctaaat
     2941 tgactaaaaa aatgaaagca attaaagctg gcgtagattc tactaaagct tatgacatca
     3001 acgaagcagt tgaagtgtta aaaaaattcg caaccgctaa attcgttgag agcgtggacg
     3061 ttgctgtaaa cttaggtatc gattctcgta aatcagacca aaacgtgcgt ggtgcaaccg
     3121 tattaccaca cggtacaggc cgtaccgctc gtgttgctgt gtttacacaa ggtgcaaacg
     3181 cagaagcagc aaaagcagcg ggtgctgact tagtgggtat ggaagatctt gcagagcaaa
     3241 tcaagaaagg cgaaatgaac tttgatgttg ttatcgcttc tccagatgca atgcgtgttg
     3301 taggtcaatt aggtcaagta ttaggtccac gcggcttaat gccaaacccg aaagtaggta
     3361 cagtaacacc aaacgttgca gaagcggtta aaaatgcgaa atctggtcag atccgctacc
     3421 gtaacgacaa aaacggtatt atccacacta ctatcggtaa agcagacttc tcagctgaac
     3481 aattaaaaga taacttaaac gcattgttag ctgctttaac aaaagcaaaa ccaacaacag
     3541 caaaaggtat cttcatcaag aaagtaagca tctctactac aatgggtgct ggtgttgcgg
     3601 ttgatcaagc atcactttaa tttttaatag aatcaaaaga ccgtagtgtt aatactctac
     3661 ggtctttttc tttaggtacg aatgttaaga ttattatctt tttgctttat ttttttgctt
     3721 agtgcttgta ctgtattacc tcaaattaag caattaccgc cacaggttga atctgtaaaa
     3781 tcctttaaag ttgaaaataa tggccagcaa agtttgcttc taattcaatt ttatgaaaat
     3841 aaatggcgtt ggattcaaac tgatccttta ggtgctccta ttgccagagt atggcttact
     3901 aaaaaaggtt gggagccaga tggctttgta atgccaaata agcaggcaca gctattattt
     3961 acagctttag tcaatgcatt aaaccctcat tctccacctt tttatttaga taaaggttgg
     4021 aaaattgaga gggaaaaatc ccaatttacg atttggctac cagatagcag taaatggaaa
     4081 gtggaaatat tgggggaata agcgaaaact tgaacctcta tgcttgaatc acttgaatat
     4141 gaattttata attttagtgc cattacacaa taatactgga ataaataata atgacactat
     4201 atttatcaga gcccgccatc accagtccga ttggatatgg catcgtggag catttgaatg
     4261 ttttaattaa tgcgaaagta tctcctttag atacgacttc acagccttat ttacgacata
     4321 atatttcaaa tagttcaccg aaatttctag ggcaagtctc tgctagattg cgtgattttc
     4381 ctgttgaact atctattgaa caccgcagcc gcaataacca actgctatgg cacgcccttg
     4441 cagaaattga gccgaaaatt accgcttgta tcgagcaatt cgggcgtgag cggattgcgg
     4501 tgatcatcgg cacgtccact accggcgccg atgaaaatat tcccgtcttc aaagccggtg
     4561 tgcagggcga ttatgccacc gagcagttta atcaacagca aattctcttc tctgcgccgt
     4621 ctgatttcgt tgccgaagtg tatggcttaa cggggctgag ttacggcatt tccaccgcct
     4681 gcacctcaga ggcaagagcg ttaatttctg ctgcccgttt actgaaagcc ggcttgtgcg
     4741 atgcggtgat ttgcggtggt gtggataccc tttcgccttt aactatcagc ggatttagct
     4801 cgctggaagt gttatcaaac caacaaacca acccattttc cgctaaccgt aacggcatta
     4861 acatcggcga aggcgcagcg gtgtttgtga tgagccgtga accactggac gaacatcgca
     4921 ttcagttact gggttatggt tccagctcgg acgcttacca tatgtcctca cctcaccccg
     4981 aaggggaagg ggcaatttcc gctttccaaa atgccctgca caatgctgat ttagacgcag
     5041 ccgacatcgg ctggatcaac ctgcacggca cagggacgct tcataacgat caaatggaaa
     5101 gcctcgctgt ggcaaaaatc ttcggtaatc agaccgcttg caccaccacc aaaccctaca
     5161 ccgggcatac ccttgggctg ccggtgcggt ggaagcggca attttgtggg gaatgctctg
     5221 tcgccaacac aacccgaccg gcaagctccc tgcgcaatta tgggataaac aagcggacga
     5281 aaacctgccg aacattgcga tcaccgatga acagagccgt tgggcagcag gcagacggat
     5341 tggcgcaagc agctcttttg cctttggcgg caataacagc gtactgattt tgggagaaag
     5401 cgatgcataa attcaccttc cctatcgaac aaattgcacc attggttcct cacagtggca
     5461 atatgatcct actcgaccgc attaccgcat tcggcgagga ttttttgacc gctgaaacca
     5521 atattcgccc tgacaatccg ctgatcaaac acggaaaact tgccacctac gccagcattg
     5581 aaattatggc acaagccggc gaaccagttc gcttaggcta cctgctcggc actcgcaaac
     5641 tgcacctcca ccgccaagaa atcccgattg gctcacagct acaaattcag atcaaaatgt
     5701 ctattcaaga tgccaccggc ttcggcgtat tcgactgcca actgatcgat ttagccgaca
     5761 accaagtgct tttagaaggg gcgctgaatg tgtttagtcc gaaatagagt tggtagtagt
     5821 aagtagtagt aagtagtagt aagtagtagt aagtagtagt aagtagtagt tggtagtagt
     5881 tggtagtagt tggtagtagt tggtaggaaa ttcgttatgc aagaggccta tgacattcaa
     5941 catctttgta ctcgcaaaga agataaatcc tttgatcgca aaagtgcgag aaaagaaccg
     6001 aaagagattt tgcggcattt gattggtttt gcgaatgcag acggcggttt attagttatt
     6061 ggtattgaag ataatggcga aattaccggt tttaactacc aaaaagccca tcagccggaa
     6121 gaatttatcc acgctctgca caatttacaa aaaatgccga taccggtgac ctatcagcaa
     6181 attgaggtgg taaatagtcg aggtttagcg gattttgtct tattatttga agttacagct
     6241 tcgagtggga gggtgattac ctctcacgat gatcgggttt ttctacgctc aaaagatcaa
     6301 actttggaac tcaattttga acaacgtttg caattagagt acgaaaaagg gcagcgtgct
     6361 tttgaagagc aaccgatagc acgcgctgat tttactgatc tggatatgga tttattagaa
     6421 cagtatcgac agaaattaca cacagatcgc tcaatagaag agattctgaa agcccgagat
     6481 ttaattaaca aagaagggca aattacgaat gcctgtattt tgctatttgg gaataacccg
     6541 acacgttatt ttcctaatgc ccgtttgcgt tttttgcggt atgaaggtaa agcacctctg
     6601 accggtagaa cttttaatgt cgtcaaagaa tttatgcttg aagagcctat tcctaagtta
     6661 ttggagagtg ctaaggcagt attaaaaagc caattacgag agttccaacg tttagatgaa
     6721 aacgggcaat tcacaaaaat tgatgagtat ccagaatttg cttggttaga aggtattgtc
     6781 aatgcgattg cacaccgtag ttacagtaat caaggggact gcattcgcat ttcgatgttt
     6841 gatgaccgtt tggaaatttt tagtccgggt ggattacctc gccctgtgac gcttgaaaat
     6901 atgtactata cccgtttttc acgcaatccg aagattgcac gagtgctgta tgaatttggt
     6961 tgggtcaaag agctgaatga aggtgttaat cgtatggcgg aggaaatgaa cgcttaccaa
     7021 cttaatacac cttcttactt tgaacccaat gaaaatgcgg tcagactgat tttagaaaat
     7081 aatattgaac atcggacatt aagggcatta gaacgaatag aagaaatttt tacgcaggat
     7141 attatggcga cattaaataa aaatgaagtt actatcctgc aatatttata tgaaaatgaa
     7201 atgattactg taaagattgc tagtgagata ttagatcgga gtaaagaatt atctcgtaga
     7261 atattaaaaa gtttagaaca aaaaaatatt ctcacttggc acggcttaag tccaaaagat
     7321 ccaacccaat actacacact caagaaatag taggaaatat aagatgaata atacagttct
     7381 catcaccggc tcaagccgtg gtatcggtaa agcgattgct ttaacccttg cacaacaagg
     7441 gtttgatatt gtggtacatt gccgttctcg tgtggaagaa gcggaagcgg tggcacaagc
     7501 ggtcagaaat ctcggaaaaa atgccagagt gttacagttt gatgtggcgg atcgtgaaaa
     7561 agtgcggtcg attttagagg cagatgtgga acaacatggt gcctattacg gcgtggtgct
     7621 aaatgcaggg cttacccgtg ataacgcctt tcctgcttta accgatgagg attgggatag
     7681 cgtgttgcgt accaatttag acggttttta taatgtcctg cacccgatta tgatgccgat
     7741 gatccgtcgc cgtaaagcag gcaggattgt ctgtatcacc tcggtttccg gcttgatcgg
     7801 caaccgtggg caggtcaatt acagcgcttc aaaagccggc attattggtg cggcaaaagc
     7861 actcgccatc gagcttgcca aacgtaaaat cacggtaaac tgcgtggcgc ccggcttgat
     7921 tgataccgat attctggacg aaaacgtccc aatcgaagaa attctcaaaa tgatccccgc
     7981 aggacgaatg ggcgatcctg atgaagtggc acacgccgtt gcgtttttaa tggatgaaaa
     8041 agccacttac atcacacgcc aagtcatcgc tgtgaatggt gggttgtgtt aactttagga
     8101 taataattcg taggggcgga tccagtaccc gcccgaagcc aaaaagagat aaatatgaaa
     8161 cgagtcgtca tcaccggaat tggtgtggtt accgcttttg gtaaagagtg ggcggaaatt
     8221 cacggctatc aaccgccgaa acattggaac cgcaagcagt tgcgcagcct tgggcgagtg
     8281 tcgcaatttg tggtggaggc cagcgagcgg gctttagcac aggccggttt gttggacgaa
     8341 aacggcgtga ttttcgactg gctaaaagac aggcgaatgg gcgtggcgtg tggctcgtcc
     8401 acaggctcta ccaatgacat caaagatgcc gccgaattgc tgatgaccgg caaatcggac
     8461 ggtttcaatg ccaacaccta tgtgcgaatg atgccccaca ccaccgctgc caatattggc
     8521 attttcttcg ggctgacagg gcggattatc ccgacttcca gcgcttgctc ctcgggcagc
     8581 caaggtatcg gttacgctta cgaagccatt aaatacggta tgatcccgat gatgcttgcc
     8641 ggcggtggag aagaattttg tgcctccaaa gtctatgtct tcgacagcct ctatgctgcc
     8701 agccgccgca acgacaaccc aaaaggcacg ccacgcccct atgacaaaga ccgagacggc
     8761 ttagtgatcg gcgagggagc gggaatgttc gtattggaag agctggaaca cgcgttagca
     8821 cgaggggcaa acattatcgc cgaagtggtg ggctacggtg caaacagtga cggttcccac
     8881 gtcacccgcc cacaagccac cacaatgcaa cgctgtatgg aacttgccct taaagatgcc
     8941 ggtattcaac cgaaccaagt cggctatgta aacggacacg gcacggccac cgagcagggc
     9001 gacattgccg aaactcaagc caccacagcg gtgttcggca aaattccgct cagctcgcaa
     9061 aaaagctact tagggcatac gctcggtgcg tgtggtgcat tggaaagctg gttcagcatt
     9121 gaaatgatgc gagacggctg gttcgcccca acactgaatt tagaaaatgt cgatgaacgc
     9181 tgtggcgact tggattacct tcaaggtgac ggacgggaaa tccacaccga ttatgtgatg
     9241 aacaacaatt tcgccttcgg cggtgtgaat acctcattga tttttaaacg ttggaaataa
     9301 ccgttttcaa ataatgaaaa agatattatt agtttattac tcccaaaccg ggcaactcac
     9361 ccatttggca gaaaattttg tacaatcgct tgaacaagcg ggcgtttttg tggaaaaact
     9421 cgcaatcaaa ccgcaacagg aatacccgtt tccgtggcgg tttatgcgtt tttttaacac
     9481 cttccccgaa accgtccacc tcacaccgcc gccgattgag cctctgccct ttcagcacga
     9541 aatatacgat ttggtgatca tcgcctattc cgtgtggttt ctctccccca gccagccgat
     9601 taccgctttt ttacagagtg aacaggcaaa aaagtgttga aggatacgcc cgtcatcacg
     9661 ctgatcggct gtcgcaatat gtggctgatg gcgcaggaaa aagtgaaaaa actgcttgcc
     9721 gagagtgagg cgaagctcat tggcaacgtg gtcaaagtgg atcaatctaa cgactgggcg
     9781 agttttatca ccacaccgct ttggatgctg accggcaaga aaaaaaatcg gcaagctgcc
     9841 gagcgctggc attgccgaaa gcgaaatgga agatgtaaaa cgcttcgggg caaaactttt
     9901 gcacattttt agccaaaacc aaccgcttga cagcaccctt ttccgccaaa tgcaagcggt
     9961 caaaattgac gaaaaattga tgatgagcga aaaagtcggt gcgagaagtt tccacctttg
    10021 gggcaaatta ctgatcaagt gcggtcaaat ttcaccgcac ttacgccaag cggtactcta
    10081 tttttacatc ctctttttag tgacgttaat tttaaccgtc gtcccgattt caacggtgat
    10141 taaacggcta ttacgcccac tgctgaaaga aaaattagaa aaacaaaaac ggtattttgc
    10201 cgagccttcg ggcgagtaaa cgtaccctac acggaaactt tatgctatct aacgtctata
    10261 tcaccaacac ctccgccttt ttacccaacg cccctgtgtc caacgatgca atggaagcgg
    10321 tgttgggaat ggtgggggaa acgccttccc gagtgcggaa gatgattttg cgttctaacg
    10381 gtattcaaca tcgttattat gccattgacc cgataacccg ccaagcgact cacagcagta
    10441 ccgagttggc ggtgcaagcg gtcaaaaaat tgggaaattc tgcaacatca gcgacttgct
    10501 tggcggtggg gacatcttac cccgaccaaa ttatgccggg gcagggggtg atggtacacg
    10561 gcttgcttga tgccgaaccg atggaagtgc tgaccactgc cggtgtgtgc gtggcgggaa
    10621 tggcagcgat gaagcacgcc tataatgccg tacgcatcgg tgagcatcgt gctgcgattg
    10681 caacggcttc cgaaaccgcc tccgccatta tgcgcagtga acatttccgt gccgaaatcg
    10741 atcataaaaa actggacgat gctcgccctg agatcggttt tgagaaagat ttcctgcgtt
    10801 ggatgctctc agacggtgca ggggcggtat tgcttgccaa tcagccgaac gaaaaagggt
    10861 tgagcctcaa aattcactgg atcgatctgg tctcttacgc taacgaaatg ccggtttgta
    10921 tgtacgccgg tgcggaattt attgacggca actacaccgg ctggaaagca atgagcgaag
    10981 cccaacgtgc cgaatgctcg gcaatggcgg tgaaacaaga tgtcaaactg ctcaacgaaa
    11041 acatcgtcaa atacacggtg gaaaaagcat tggagcggat tatcccgaaa tacaagctca
    11101 acgctgaaga gattgactac ttcctgccgc actactcgtc cggttccttc cgagataaat
    11161 tattggaagg tttacagcgg atcaatttcc tgatcccaca ggaaaaatgg ttcaccaacc
    11221 tgactaccaa aggcaacact gggtcagcct cgatctacat tatgctcgat gaatttatcc
    11281 gcacccacga tctcaaacag ggacaaaaaa tcctctgcta tgtgccggaa agcggacgtt
    11341 tttcgtcttg ttttatgctt ttggaagcgg tggtaaattc gtaaaatcgt agggacgggg
    11401 gctattcccg tccaacaatc atcggaaaac gtatggacat caatgacatt ccccaagaca
    11461 acagcaaaat cttcaacggc gaacgcaaag tgatctacgc ccgcaatccg caaccagaaa
    11521 tccgccaaat gatctcctct attctcgctt gctacgcttg ggacgaaaac aacccgtttg
    11581 tagcgaagtc tgatgcacgg ttgaaggcat tggcggaagt tgttggtaaa gcagaaattg
    11641 gcgattaagt ttgattttgt gctttacagg gtcgcagatt atctgtataa tttgcgacct
    11701 taacttgcga gagcaagctg atggtgctta gcctatctaa gccccgtcta agactgtagg
    11761 ggtgaaaact taataatcct acatagatgg tgaacagaca gaattctctg cttctggaca
    11821 ccttaggctc aaagaagatt gcagtttgta gcaatgcaag cggtaatatt tttgggattt
    11881 tttgtaaatt ctgcttgaaa aggcagagtg tatcaggagc taaaaccaat ggcattaaat
    11941 cttcaagaca aacaagcgat tgttgctgaa gtaaacgaag ctgccaaagg tgcactttca
    12001 gctgttgttg cggattctcg cggtgtaaca gttgaaaaaa tgactgagtt acgtaaagca
    12061 gctcgtgaag ctggtgtgac aatgcaagtt gtacgtaata ctttattacg tcgtgcagtg
    12121 gaaggcaccg agttcgagtg cttaacagat acgtttaccg gtccaactct tatcgcattc
    12181 tcaaatgaac acccaggtgc agcagcacgt ttattcactg aatttgcgaa aacaaacaaa
    12241 gagtttgaac ttaaaggtgc agcctttgaa ggtaaaaaac aagatgttgc attcttagca
    12301 accttaccaa cttacgaaga agcaattgca cgtttaatgg gcacaatgaa agaagctgcg
    12361 gcaggcaaac ttgttcgcac tcttgcagca ttacgcgatc aaatggaagc agcagcttaa
    12421 ttttaagcac tcttctttat tcgtttaaca acttatttta ctttaggaat cgattgttat
    12481 gtcattaact aacgaacaaa tcattgaagc gattgcttct aaatcagtat cagaaatcgt
    12541 tgaattaatc actgcgatgg aagaaaaatt cggtgtttca gcagcggcag cagtagcagc
    12601 agctccagca gcaggcgcag cggcagcaga agagaaaact gagtttgatg tagtattagc
    12661 agaagctggt gcaaacaaag ttgctgtaat caaagcagta cgtggtgcaa caggtttagg
    12721 cttaaaagaa gctaaagact tagtagaatc agctccagct gcattaaaag aaggcatctc
    12781 taaagcagaa gctgaagcac ttaagaaaga attagaagaa gctggcgcga aagtagaaat
    12841 caaataattt ttgatttatt ttgtcagaac ttcaagttca acaaaccccg atggaaacat
    12901 cggggttttg ttttttcaga attgtgtaat gtagatatga ttacctttat ccattacttg
    12961 cgcagcagtg aaaaatgtaa aaattccaca aagtgatctg cccagtgcat ttcgtggtgg
    13021 atttcatttg ccataatccg caggcggata ttgtctaacg gcacgccaat ccgaatcagt
    13081 gcttggtagt aattgagcga acaatcaata taggtttggt tcgtattaga gataaagtgc
    13141 gaatcgattt catcgccttc gttagtgcca acctgaataa acactttggt attgggttgt
    13201 aacggatatt ggtgcgtaaa acgcagaaaa tccggctcac taaaccaaga ggcaagcgaa
    13261 aacaccccta aatgcccgaa aatatgtggg tagcggctgc ccatataagc ggtgataatg
    13321 ccacccattg aactgcctgc caataaggta tgttcttgct gaggcttggt gcgatagtga
    13381 atatcaataa agggtttaac cgtttccacc acccatttgg catattcctc ccctaagcct
    13441 ccggcatttc gggtttcagg ggttgagccg gtggttgttt tccacgggcc gtattcattt
    13501 aagcgattag ctcctgcgtt atcaatgccg acaataatca tcttcggtaa ttcttggtgt
    13561 gatttaatgg ttggaataat tttccaagaa tgccccgaat aagactcttt gctgtaaaac
    13621 acattttgcc cgtcgtgcat atataacacc ggataacgtt cccaaccttc gttgtgataa
    13681 tccttcggca atagtacccg aatgcggcgg tgatggtgta aatagggaac atataaaaaa
    13741 tgctcctgca tttctaaata gtggttaagt ggtttttgta ccatcagtct gtccttttgc
    13801 ttaaattgtg gcgtatttta caccttaatc agtctgagtt aaaacaagcg gtcagaaaaa
    13861 gtcaaaaatt tgcaaattgc tgttacctaa cgcaatcggt tgccttatgc ttttgaggaa
    13921 tataaaagag aaactgtgat ctaagtcaca gcaattttta ttcggtttga ctaaaatccg
    13981 aactatcact taatagagag gaaaatgtta tgcaggcttc attacaagat attcttgagg
    14041 tagtaaaagc aaaaaatctc acttatcagc aaaaattgat gagtttaggc aatattgctg
    14101 agcggttatt taacccgatt gaactacttg gctacactga agaagagtgg gaacacattg
    14161 aaaatcagat gatttgcgat ttaaatgaag gttatgcgat ttatcgccca cgttatattt
    14221 tgcctgatta taatgtgtat atgcaaaaag gttgccaatt cttagattta cctccgccaa
    14281 ccaatttaga tgaagcctta gacggcttgt taattctgta ttcacacgtt ccgtccatta
    14341 ctacttttcc ggtgtatatc gggcgtttag accaactgtt agaaccgttt ataaccgatg
    14401 aagagcaaga ttacattaaa atcaagcgtt tcttaaatca tatcgataaa accatttccg
    14461 attctttctg ccacgctaac atcgggccga atgacaccaa agccggtcgt ttaatcttaa
    14521 aagcggtgat tgagcttgaa aacattacac cgaatatgac gattcgttat gataaaacca
    14581 aaacatcgcg tgaatttgcc gaactggcgg cgaaagcgtg cttgttggtg tccaaaccgt
    14641 cttttgctaa tgatgcctac tacattcaag atttaggcga agaatacgga attgcaagct
    14701 gttataacgc cttaccggaa tgtggcggtg cttacactct gacccgcctg cgtttaggta
    14761 ctatcgggcg tgcttgtgcg agtgttgaac aaatggtcaa tgagatgctg ccgaaagtgg
    14821 caaaactcgc cctttccacg atggataaac ggcataaatt cttggtggaa gaaagcaatt
    14881 tctttgaaag tagcttcttg gttcaggagg gctttatcaa acgtactaac ttcacaagta
    14941 tgattgcgat tgtaggtctt gctgatgcgg tgaatcattt actcaagcaa gaaggtttaa
    15001 atgaaacctt tgggcaaagc aaacgtggcg atgaaattgc gacgctgatt atggataagc
    15061 tccaagcagt aaatgacgct cataaagggc tgtatgttga acgaaccaat aaccaatatc
    15121 tgttacacgc tcaagtgggg gcaaataacc acgctgaaga caaaatgaac acgccggcac
    15181 accgcattcg tgtgggcgaa gagccaacct tattggcaca cttaaaacag tctgctcctt
    15241 tccataaata tttcccatcc ggtacggggg atttattcgc ctttgaccaa acctacactg
    15301 atcatttaga tgcggtggtg gatattattg acggctcgtt cgcccaaggt tatcgctaca
    15361 ttaccactta cctgaaaaat actgatttga ttcgagtgac aggctacttg gtgaaaaaaa
    15421 gcgaggttga acgttatcgc aaaggcgaag cggtattgcg tgataccact tggtatggtt
    15481 caggcaccga tgattgtgcg aaagtgttcg accgccagct gcgtgatgaa gaaaatgtga
    15541 atgcagggta acaatggctg cactttctga gatttttgtg ccgttacacc gcattatccc
    15601 cttctcgaat gtagaagggc agggcaatcg taccagcatt tttctacaag gttgcaagct
    15661 gaattgcctg tattgccata acccggaaac cattccacgt tatagcaagg aagcaaaaca
    15721 ggttagcttg caatacttgt acgatcaagt gatggaggca gttcccttta ttcgaggagt
    15781 cactatttcg ggtggagagc caaccattca tcataaaaaa ttagtgccac tgttcgaggc
    15841 gttgcgtgag caagggctaa cctgttattt agacagcagc ggcttttttg attttgaggc
    15901 aacagagccg ttaattaatg ttaccgataa atttctgttc gatctcaaag gagatggggc
    15961 gggcttacaa acgctatgct ttgatcggaa aaaccaagcc ggcaaagtgc ctgaacaggc
    16021 tcttccaaac gtgaatcata ttaaacctga aaacttggct cgcaatttgc aaaatttagc
    16081 caaattatta ccgcttgata aggtggaaga agtacgtttg gtttacctca atgagtttta
    16141 taacgcccac catttgctcg aaaaagtggc tgacctactt aaaccttacc cagatgtact
    16201 acttaaaatt attcgggtac acaccaaagg agcgagagat gaaacaggct tggctcgttt
    16261 tgtacccact atcgagcaaa ccgatgaact ggcagcattt gcaaaacggc acggtattca
    16321 aaaagttatt actatttact aatggagact tacgatggat atactaatta gcataattgg
    16381 tgtttttgtg ttattaggct taggtgtttt attatcaaat aatcgcaaag ccattaaatt
    16441 tcgcacaatt ttgggcgctt tggcgattca aattggtttt gccgctctta ttctttactt
    16501 tccagcggga agaaatgcat tgctagctac tgcaaattgt gtgagcaata ttattaatta
    16561 tggtaacgaa ggtattagct ttgtgtttgg caatttagcg aatccgagta atagcagcat
    16621 tggttttgtg tttgcggtta aagtattgcc tattattatc ttcttctctg cgttaatttc
    16681 aatgctttac taccttggtg tgatgcaatg ggtgattaaa attattggtg gagcattaca
    16741 aaaaacacta ggcacttcca aggcagaatc aatgtcagcg gcagctaatt atttttgttg
    16801 gtcagacaga agctcctcta gttgtaaaac catacattag ccgtatgaca gagtcagaat
    16861 tatttgccat tatggttggt ggaactgctt caattgctgg ttccgttatg gctggttatg
    16921 ccggaatggg cgttccttta acttatctta ttgcggcctc ctttatggct gcaccagctg
    16981 ggttactatt tgctaaatta atgtatcctc aaacggaaac cttggagagt aaagcagatg
    17041 acaatgttga agttgaaaag ccaaataacg tggttgaggc cctagctaat ggtgctagtg
    17101 caggtgttac tttggctatt aatgtcggtg ctatgttaat agcatttatt gcagtgatcg
    17161 ctttattaaa tggctttatt ggtggcattg gtaattggtt tggttatgag gggctaacct
    17221 tacaaatttt attaggttat ttattccaac cacttgcttt cttaatcggt gtgccgtggc
    17281 aagatgccga tattgcaggt caaatgatag gtatgaaact tgctgttaat gaatttgtcg
    17341 gatatcttga gtttgctaaa tatctacagc ctgatgctgt ggtgcaatta agcgataaaa
    17401 ctaaggcaat aattactttt gctttatgtg gttttgcaaa ctttagagca attgcggtat
    17461 tgattagtgg tatcggaagt atggcaccaa gtcggcgttc agatattgct cgtttaggtt
    17521 taaaagcagt gattggcggc acattggcga acttaatgag tgcaactata gcgggtttct
    17581 ttattggttt aagtggtgcc acattaggtt gatacaagcg gttaaattga ctcacttttt
    17641 tgcaaaaagc ccaagtaagt taaatgacta cttgggcttt gtttaatgaa ctagctctta
    17701 gtttcggttt ttgctgtgtt atccttttcc gctttggctg tctgtgtttt cgaagttgtc
    17761 tgtttttttg cggattggct agcagtagct gcttttctta ctttctttgt tttaacttct
    17821 acttccgcat ttttagagtt tttatccgct aaccatgcct gcactttagc taagaatatt
    17881 tgtcccatcg ggcgaggatc gccagaaaat aatgtttgta atttattatt ttcaataatg
    17941 tcgcgtctaa ttgctaagcg ttcttcgtgg ttttccgcta aacgaatagc ttgagtaacg
    18001 tattcctctg ctgtttgggt aattaaccaa ttaggtagcc cgaggcgctt aaataaacct
    18061 tcatcaatat gttcatgaac ttcagggcct gttttacaga caccaactaa accaagcgta
    18121 accatatcga taatgccgtt tgtatttcca aatgggaatg gatttagcat catatcgcaa
    18181 ttatgtagaa tatttagata ttcatcatat ggcgaatgtg ggtgtgcggt tgcatcattt
    18241 cctaaatagc ttttgataaa acgttccaca taaggatgcg taattccact agattgccct
    18301 aatgcaaaat ggaaatgagt ttttaccttc gccctatcac gaatggcttt taatgcctct
    18361 aagaaatatg ggtttaattt catagtggtt gatgcaattc cgatatggat aacctctgga
    18421 ttttcacgta ggttatagac cacattttga ggcgctaatg cggaaggcac atagggtagc
    18481 gcatcttttg gaagacgtaa taattgttca ctaaagcagc tttcggaacc aacatagtca
    18541 tcttctacga tgacatattc aataaaatca gagtgtgtag ttgccggatg acctagcgca
    18601 acagcttgaa ttggtgctag gcgagtgtta ctagcgaaaa tagtggttaa atccattcca
    18661 atgctaggca tatagaatac ggctggatta tttttatcac agatatcttt taaaaagctt
    18721 aagcggtcaa atagtgagcc atcttgtggt aataaatgaa attcgtcgaa tacctgttgc
    18781 cccatttcat ctacagcatc actgcctagc ccaataagat ggaaatgttc gcgtgcagca
    18841 accattgagg ttgaatgagt acgataaata gaatgtgatg aatggaaatg ctccaataat
    18901 actaccatga caggtttatt atttctggta ccaagctgtt caatttttct atcttcccaa
    18961 ccgacagaaa gtaaatgacg acgaattaca gcatttaacg accgttttac attatgttta
    19021 tttgcttcaa tatcataact gcaatgcata taaacatcgt gtgaaatggc gctcggtagc
    19081 ttgttcaagt tttcaatttg cgctaatttt tctgggaacc attgcaaaat agccgcgcgt
    19141 tttgcaaaag cagaggatgt accaataaat ctaggtgatt ggagtgcaaa acaaagtgat
    19201 gcggtgagat ctgatgaagc attccatgca gcatctagat tcaataaaat attagattcc
    19261 ggtaagtata aaatacagaa cttaattagt gcttgtaatg tggcatctaa atcaaccgta
    19321 tttggattag cagattcttt atttctattg taagtacgca agatatggtc tgcgtttacg
    19381 tatggagaac tggcaaaaat taatgctatc cagcgttgta atgtcagaaa acgcatagca
    19441 ccgtgttcgg agatctctaa tgcaggatct tcaaacaagc gagtaatcgc taccgccatt
    19501 cgagtacaga aatagatagc tttatcattt tctaaatttt caatctgctc tggcatattc
    19561 aattcaatat tggaaatgcc accaaaatta ctatctaatt ttgaaaggat agacagtagc
    19621 tcggtacaag ccgattcata atcttttttc gcgaccgcct gctcaaaacg gatgacgcta
    19681 ggcatatttt ctgctgacat aatgtatcct tagttgttta aaattcccca cagatcatac
    19741 tcatctgcat gtgtaatggt tactgaaata atttgcccta ctgtcactaa ttggtttgaa
    19801 ggattatcta catagactac accgtcaatt tccggtgcat ctgccattga gcgaccgata
    19861 atgccttctt cgtcaatgtc atcaataatt acttgtacgg ttttgccgat ttttgcttgt
    19921 aagcgagcag cggaaatttc ttgttgtagt tgcataaaac ggtggaaacg ttcctctttt
    19981 acttcttccg gaacttgatc cggcatatcg gtagcaacag caccttccac tgggctgaat
    20041 ttaaagcaac caacacgatc taattgtgct tcttttaaga aatcgagtaa tagttggaaa
    20101 tcttcttctg tttcccctgg gaagccgaca ataaaggtag aacgcagagt taattcaggg
    20161 caaatttcac gccatttttt aatgcgttct aaggttcttt cgattgaacc cgggcgtttc
    20221 atcgctttta agattttcgg gctggcgtgt tggagtggaa tatccaaata cggcaaaatt
    20281 ttgccggcag ccataagcgg aattaaatca tctacgtgtg ggtatgggta aacatagtgc
    20341 aaacgcaccc acacgcccat tgagcctaat tgctcacata gcgtaattaa attactttta
    20401 atcggcatac cgttccagaa aacggttttg gttgcttctt cttttttgcg atctaacgaa
    20461 taggctgagg tgtcttgtga gacgattaac agctctttta ccccggcatc aactaggcgt
    20521 tttgcttcat ctaatacttg cacgatagaa cggcttgcta aatcgccacg cattgaagga
    20581 ataatgcaga aagtgcagcg atgatcgcag ccttcggaaa ttttcaaata agcgtaatgc
    20641 ttcggtgtta atttaatccc ttgttttggc actaagctgg tgtaagggtt aaattccggt
    20701 tttggcacat atttatggac gtggttcatt accgcctcat agctatgtgg ccctgtaatt
    20761 tccaatactt tagggtgtac ctcacgaatt tggttttctt ttgcccctaa acagccggtg
    20821 acaattactt ttccgttagc ttctaacgct tcaccaatgc tttcaagaga ttcttgtact
    20881 gcggaatcaa taaaaccgca ggtgtttaca atcacaagat ccgcattttc ataactaggg
    20941 atgatgttat aaccatccgc acgcagttcg gtcaaaatac gttcggaatc aactaaattt
    21001 ttagggcagc ctaagctgat aaaaccaata tttggtgaag cactcatata actctcaata
    21061 actaaaaaga aaaatgccta caatttttgt ctgtaggcaa aaaactaaca agattctaca
    21121 aaatcttgcc caaaatagcg ataacaagcg gtcaattttt aataaacatt tacaaacttg
    21181 aaatttcgct aacaattaaa attaatcgat attatttagc attgctaata caatcgtgga
    21241 agatttttct gcggctaatg gcagaaattc ttcaaaagaa aggtgggatt ctttgtccgc
    21301 tacatccgat actgctctta cgattacaaa tggaaggtta aaaccgtgac aaacctgcgc
    21361 aatggctgct gcttccattt cgactgcaac gacattcggg aaattctgac gaatttgatt
    21421 aatcttatca ttaccgttaa taaaggcatc gccgctacag attaagcctg aaaccacatt
    21481 gtaaccggct tttttcgctt ccttttgagc taattcgact aattgctcgt ttggcgaaaa
    21541 tgttggcggg tttgccggta gttgcccgat ttcataacca aaggcggtca catcaacatc
    21601 gtgataacga acttcggttg aaatcaaaat atcacctacg tttaaatctg gatctaatcc
    21661 accggcagag cctgtattaa taatcatatc cggctgggtg agctgtatca gtaaggtagt
    21721 gccgatggcg gcagcaactt tgccgatacc cgattgtaat aatgccacac gggtattgtt
    21781 aattttgcct tcaaaaattt tacaacctgc taattcagtg actttaggtt caaccattac
    21841 ttggcgtaaa atctcaattt cttgtgccat cgcaccaata ataccaattt tcattacttt
    21901 ccttctctaa tgtattgttt attttttgaa aaaaacgcta ttttaacagc attaatagtg
    21961 aggagggggt gtttcttctg cttgtgaagc aacttggctt agttgagccc ctttcaattt
    22021 ctcagcaata tggcgaatct gagtttgtaa cttatcaatt gcaaattgtt gctcaataag
    22081 tgcttgattt aactcttcaa taatcagctc ttggaaagca actttagtct ccaatt
//
LOCUS       DANLUA010000016        21338 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLUA010000016 DANLUA010000000
VERSION     DANLUA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..121
                     /locus_tag="PXB44_000511"
     CDS             <1..121
                     /locus_tag="PXB44_000511"
                     /inference="COORDINATES: protein motif:HMM:NF013463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5281023.1"
                     /translation="SFGSFNHPQFYQLISPNGELCIVPFQSRQHLDYLAQKHH"
     gene            152..2020
                     /locus_tag="PXB44_000512"
     CDS             152..2020
                     /locus_tag="PXB44_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL5281024.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(2180..3076)
                     /gene="malM"
                     /locus_tag="PXB44_000513"
     CDS             complement(2180..3076)
                     /gene="malM"
                     /locus_tag="PXB44_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL5281025.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3157..4446)
                     /locus_tag="PXB44_000514"
     CDS             complement(3157..4446)
                     /locus_tag="PXB44_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL5281026.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            complement(4544..5662)
                     /gene="malK"
                     /locus_tag="PXB44_000515"
     CDS             complement(4544..5662)
                     /gene="malK"
                     /locus_tag="PXB44_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL5281027.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            6083..7273
                     /gene="malE"
                     /locus_tag="PXB44_000516"
     CDS             6083..7273
                     /gene="malE"
                     /locus_tag="PXB44_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL5281028.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            7392..8930
                     /gene="malF"
                     /locus_tag="PXB44_000517"
     CDS             7392..8930
                     /gene="malF"
                     /locus_tag="PXB44_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL5281029.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            8983..9873
                     /gene="malG"
                     /locus_tag="PXB44_000518"
     CDS             8983..9873
                     /gene="malG"
                     /locus_tag="PXB44_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL5281030.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            9948..12005
                     /locus_tag="PXB44_000519"
     CDS             9948..12005
                     /locus_tag="PXB44_000519"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL5281031.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(12119..14845)
                     /gene="malT"
                     /locus_tag="PXB44_000520"
     CDS             complement(12119..14845)
                     /gene="malT"
                     /locus_tag="PXB44_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL5281032.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSMNESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            15009..17378
                     /gene="malP"
                     /locus_tag="PXB44_000521"
     CDS             15009..17378
                     /gene="malP"
                     /locus_tag="PXB44_000521"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL5281033.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            17460..19538
                     /gene="malQ"
                     /locus_tag="PXB44_000522"
     CDS             17460..19538
                     /gene="malQ"
                     /locus_tag="PXB44_000522"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5281034.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(19603..21264)
                     /locus_tag="PXB44_000523"
     CDS             complement(19603..21264)
                     /locus_tag="PXB44_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL5281035.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
BASE COUNT         6392 a         4428 c         4130 g         6388 t
ORIGIN      
        1 atcatttgga agctttaatc atccacaatt ttatcaactt atttcaccaa acggtgagtt
       61 atgtattgtg ccgtttcaaa gccgccagca tttagattat ttagcacaaa aacatcatta
      121 atcttattta tattatacag gtgggaattc tatgtcagga ttagttagtc cggaattaat
      181 taaaacagtc gcattacttg caaccagcgt cactattgta ccgctattta aacgccttgg
      241 tttaggaagc gtattgggtt atttagtcgc aggatgttta atcgggccat cgggcattgg
      301 attgttccaa gaccctgccg ctgttgtgca tatggcagaa cttggcgtgg ttatgttttt
      361 gtttattatt ggtttggaaa tgcacccgga actgctttgg gcgatgcgta aagccatctt
      421 cgggcgaggt ttcttacaag ttgcgatttg cggtacttta ttaaccttag ccggcattta
      481 cttattaggt ttttccaaag aagtggcatt tattgccggt atgggtttta cgctatcttc
      541 tactgccatt gtaatgcaag tgctggaaga taaagggata agttccacgc caaaagggca
      601 acgtattgtt gcaactttac tatttgaaga tttagcgatt gtgccattat tagcctcggt
      661 tgcctttctt gctcctaacc aagtagatgc tgaatccagt actaattggt ctgctattgg
      721 tcttagccta gttgccgttc ttattttggt tgcgagtgga aaatggctga tgaaccctat
      781 tttccgtgtt atttctaaag ccaaagtacg agaaatgatg actgccgctg ccttacttgt
      841 ggtattaggc tccgctcttt taatggaaat cagtgggctt tcaatggcaa tgggggcatt
      901 ccttgccgga gtaatgcttt cggagtcttc tttccgccat caactagaag ctgatattga
      961 acccttccgt ggtttacttt taggcttatt ctttatgggc gtaggaatgt cattagacct
     1021 cagcctcgtc ctcaataatt ggctatggtt attggtgatt gtagcagttt atgttatcgg
     1081 taaaggcctt ggaatcttca ttgctgcctc tatcacccga ttaacactga atgaatctat
     1141 tgtgcgaact accattatgg ctcacggtgg tgaatttgcc tttgtgctat tctcagccgc
     1201 cgcaaccgct gggattttaa ccccagatac tcacgctacc ttaaccgctg cggtaattgt
     1261 ctcaatgttg ctttccccac ttctcatttt aatcaagcaa ggagtgagaa agaaaaaagc
     1321 gaaaaatgaa tccactaata tgacaggggt tgaattaccg gaagatttag aagccaatgt
     1381 attagtcatc ggttttggac gctttaacca aatagtctgc caagccttat tagccaaagg
     1441 actaagtgta tcggttattg attcaaatac tgatcatatt cgtgctgctg ctcgttttgg
     1501 ttttaaactt tactatggtg atggagcgag actagatgta cttagggcat ctggcatcgc
     1561 caatgcaaac tgcgtaattg ttggcgtagg caatccggaa agaacggaga aaatcgtaga
     1621 gctgattaaa tctgaatacc ccttagtacc tgtatttgcg agaacctatg atagacaaag
     1681 tgcggttgat ttagtcaaaa agcaccacgt tgattatcac attcgagaaa cctttgaatc
     1741 agccctcgca ttaagccaag ccgccattga acgcttaggt tctagcgagg aagaggcaaa
     1801 acaaattatc agctatgttc gccgattaga tgcagaacgc tttaatgagg aattattaca
     1861 cggtttctcc gcagaagtta tccgcaaata ttgggtacca acaccttggg taaatccaca
     1921 acacgaaggt agtgcactta atgaagaaac ggctgatatt ttagaagagc ttggtgagac
     1981 agtagaagtt ataaaagtgg atatcactga taaaaattaa agaatttata ctttgaatcc
     2041 ttattcaagt ataagccttc ctatacatat cgcataatca ctcgtaaaaa agcaccctat
     2101 cggcataatc tgatagggtg ttaatggtaa taaaccatcg cacgatgaca aggagaatat
     2161 ctatgaaaaa aacaataaat caatttgaac ttacattttt taagaaaatt tggcgtggag
     2221 aagtcagccc taatttttct gcctcgttaa ccaaattcat cgctttatta atatcttttg
     2281 ctttaatcgc tttcattacc gcctgattaa aataggcttc cgtatcttta tctactgctg
     2341 tttttactgc aactggattt tcttttatcg gcaacggtat agccgtttta gctgccggcg
     2401 ttgctgcttt gtttgagctg aaaatttcag tcggtaatcc aataaattta gtgccgttcg
     2461 agccggtaac gttgatgtga atttgccctt ttaaactgtg tgctacttca atatcgttaa
     2521 ttgctggtgg ttgtttaccg gttgcttttg cataggtttt tgccggatgc ggcatcatcg
     2581 tggttttggc taaatcttgc tgcgtggtat aaatgagcaa gtaaatataa tcttggttca
     2641 ttgccggcgt taaattaagt tctgccccta aacggctacc tttgagtcct cgctcttcca
     2701 ataatttaaa ctcagaagag gggtaagttg atgccacatt aaattgacta tccaacacga
     2761 ctgccgtggg tacaaaaaca tttttgtcca tcaccgggct ttcgatttca atttccaatg
     2821 tgccttgatt tgccggaata cgataagctg caaccgggct ggcaataccg gcaaacgctg
     2881 tggtaaatgc ttgtttgtgt tgctcgctta gatctgtttt tacggtttgt gaaaatggca
     2941 catcttgcca ttgaatttga gctaattcag ccgagttaat atgaatagac ttacctgccg
     3001 agccatttgc tatactcgga gacaccacca aaagatgggc gaagaatatt gctgtcgaaa
     3061 gaagcgtttt tttcatatta acccctaaaa atcgtgaaat aggataaagt gctgaaatag
     3121 tgggtaggta atcctaccca cacaaaggtt tgatgattac caccaagctt caaattgaac
     3181 accggtgata aactcgccgt ctttggcttt atagccggca tcggtacgag ctttcgtgtt
     3241 gaatttatca ttccaacgtg cataagtacc aaacacacgg attgcagggc gagcccaaat
     3301 gctattacca gcctgccatt gttgtgcaac ggtgtatttt accaagtcat ttttcttgcc
     3361 tgttgcttga tctttaatgc gatcataacc aacttctaat aatgtactca ttgtatcatt
     3421 ccatttgtac attgggcgaa tacctgctga gtaccaagtt ttaccttgtt tattatctag
     3481 ttttgttttt tcgtagatta aggcatacat tacttcaact ttatcactcg cttgcactac
     3541 cccttggttg attaaacgaa gcatattacc tttattatct gctttcgaac cctgtgcgtg
     3601 cccattattc caagaagtca tagagtcggt tgcatattgt gcagtaaatt tattgaaacc
     3661 accaaagaag ttaccttgag tgtattccgc tgttaccata taaccgtttt tggttgctcc
     3721 tttttcaagg gctgcacctt tcttagcatg tgcattaccg taatcaaagc ctaactctaa
     3781 cgaaccatct ttccaaagct caataccggc taaccgaaca tcaaaaatat cgttgtaaac
     3841 atcggcttta ttacgctgct tatctaattt aattttacct gtgctaggat ctaatttata
     3901 aactgtagta tatccaaaag tattcgcccc tccacgctct gtatcacgag taaccgctaa
     3961 agagagtttt ccgaagccta aatcaatatt ttcaacccct gcacccggac ctgaaatatc
     4021 ccagtaatag aagtcattca tatgtacatc atggcgttgg taaaaacgtt taccagccca
     4081 taaggtggca cctggtaaac tatctgcaaa gtttttgaac tgtacattta attcacgtag
     4141 tgctggactt gttggtgtcc aatcattatt gtgaagcgta ccgccgtaag caaggtttgt
     4201 atcaaagtag atagatttct caccactttt gaataattcc tgacctaatt tcaattctgc
     4261 ataggtatca gattcgttac ctaaacggta ttttgaacca ccaccattta ccgtgaatgc
     4321 ggattgctcc cccccccccg atgtccaacc gatacctgaa cgtgcatatc cgtggaaatc
     4381 aaccgctgat gcactggctg cgaacattgc accactgatt gcaactgcta ggactgtttt
     4441 tttcataata gaacctctct taagatttga ttttacttag gttaattatt aattgattag
     4501 aagattgaaa ataccctcta aaatcagtaa taaactgaaa ttgttatacc cctttttcaa
     4561 caaacaaacg tttgcaagct gtaccgtctt ccttaaataa atggcagcgt tccggcaata
     4621 taccgatatt cagatcgtcg ccttcattaa ctaataccac atcgttttga cgataaatta
     4681 aggttggttg tttgatttca gggatttcaa ggtggatttg tgtttcattt ccgagcaatt
     4741 ccacaacttg cacaataccg tgtaaggtca cttgggtttg atccgaagga actaaatgtt
     4801 ccgggcggat acctaaagat aagttatctc ccacattcac cccttcgccg gaaaccggaa
     4861 tccaaaaatt atgatgattc gcatcaggta actcaatttt tacccgttcc ggctctaccg
     4921 caatcacttt taccggtaaa aagttcattt taggcgagcc gataaaccct gctacaaagc
     4981 gatttgccgg gtaatgataa agttctaacg gtttacctac ttgagcaata ccgccggcat
     5041 ttaataccac gattttgtcc gctaatgtca ttgcttcaac ttgatcgtgc gtaacataaa
     5101 tcattgtgcg gtttagtttt ttatgtaatt tggaaatttc tacccgcatt tgtacacgca
     5161 atgcggcatc aaggttggaa agtggttcat cgagtaaaaa tacttccggt tgggaaacca
     5221 gtgttctacc aatagccacc cgttggcgtt gtccgccgga aagtgcttta ggttttcgat
     5281 ctaataaatg ggcaagctgt aagatttcgg caacttggtt aactcgttga tcccgctctg
     5341 ctttacttac acctgccagc tttaagccaa aagacatatt ttcggcaacg tctaaatgtg
     5401 gatagagagc ataggattgg aacaccatac cgataccacg ttttgaaggc tctacttcat
     5461 tcatcagttt tccaccgata tagagttctc ctgtcgtaat atcttccaaa cctgctatca
     5521 ttcgtaatag cgtagatttg ccacaacctg acggtccaac gaaaacaacg aattctcctt
     5581 cgttaatttc taaattaata tccttagaga tatgaacatc accatacgat tttcccacgt
     5641 tacgtaatga tacattcgcc ataactaaac ctcttttctg cttttgcttg ttggttttat
     5701 ttaggctgaa tagtcgcctt tttatctaaa gaagaaatca tcctaaaata ctttttttta
     5761 ggctgaatag tcgccttttt atctaaagaa gaaatcatcc taaaatactt ttttttaggg
     5821 ttagagccat agccataatt atttaaaaaa cgccaattca gaaaaattaa ataaattttg
     5881 tgatcaacct ctcattttta atgcctattt ttgtgaagca gatcacaaaa tgataaaaaa
     5941 tgttacttag atcatacttt tagcttgggg ggcgtagagc aaagggtgat ctcaaataga
     6001 ttgtatagcc cataatttgc cataatttaa taaatgggtt gtttcatatc cgtttctttt
     6061 atttaacttc aaggagtttt ttatgaaaaa caaatgtgtc aaattcacct tagctactct
     6121 tgctacctta gttctttcag gaacagtaat ggcaaaaatg acggaaggta aactcgttat
     6181 ctggattaac ggtgataaag ggtacaatgg gcttgccgaa gtcggtaaga aatttgaaaa
     6241 agataccggt gttcaagttt tagttgaaca cccggataga cttgaagaaa aatttgcaca
     6301 ggttgcctca actggtgacg gtccggatat tatgttctgg gctcacgacc gctttggtgg
     6361 ttatgcacaa tccggtttat tggctgaagt gaatgtaagc aaagaattta aagataaatt
     6421 tgtcgatttt gcttgggatg ctgaaactta caacggcaaa attatcggtt atccggttgc
     6481 gattgaagcg atttcattaa tttataataa agatttagtg aaagaagcac caaaatcttg
     6541 ggaagaaatt attgagttag ataaaaaact caaaaaagag ggcaaaaatg ccattatgtg
     6601 gaacttatcc gagccctatt tcacgtggcc gattgcggct tcaaacggag cttatgcatt
     6661 caaatttgct aacggcaaat acgatccaaa agatattggt gtgaacaatg aaggggcagt
     6721 aaaagcacta caatttgtag tcgatatggt gaaaaataaa catattagtg cggatatgga
     6781 ctatgccgtt gccgaagcct cctttaataa aggacaaagt gccttaacca tcaatggtcc
     6841 ttggtcttgg ggcaatattg ataaaagcgg tattaaatat ggtgtaactt tactcccaac
     6901 attaaacggt caagcttcta aaccattcgt aggggtgtta agtgccggtg taaacagctc
     6961 aagcccaaat aaagatttag ccaaagaatt cttagagaat tatttactca ccgacgacgg
     7021 tttagatgca gtcaataaag ataaaccttt aggtgcggtg gcgttaaaat cttaccaaga
     7081 gaaattagcg gctgatcctc gtattgctgc aacgatggca aatgcgaaaa acggtgaaat
     7141 tatgccaaat attccgcaaa tgtcttcttt ctggtatgcc gaaaaatctg ctatcaataa
     7201 tgcagtgacc ggtcgtcaat cggtgaaaga agcattagat gatgcacatt cacgcattca
     7261 aaaacaacaa taattcttca tcattctgag tgggttatta tgacccactc tatttttaga
     7321 ttcaccctaa atacaataaa agtacagtaa aagtaccgta acaaaatgca gtaaaaattc
     7381 gaggtgctgt tatgctaact caaactcaat ccaaatccac tcattggctc aaatatctct
     7441 tagccggatt agtcttgctc cttgacttct atcttgttgt cttaatgtac tcacaaggtg
     7501 aatatctttt tgctatttta acgctgatta ttctaacttc cggtgtctat atttttacca
     7561 ataaaaatac ctatgcttgg cggtatgttt accctggcat taccggaatg gcaattttta
     7621 ttttgtttcc gctggttgcg actattgcaa ttgcgtttac caactatagt ggttctaatc
     7681 aattatcctt tgaacgagcg gtatccgtct taactgaaca acgctatttt gccggagata
     7741 aatatcaatt caccctttat ccgcaagcag ataataaata tcaaattgca ttaaccaatc
     7801 cgacgacaga gcaaactttt gtgtcagagc cgatttcatt ggctaccgga acgaatgtgg
     7861 ttgtaaccga gcaaaacaga ccaactggcg aagtcgctcc ccttaaagtg attacgcaaa
     7921 atcgggccgc acttcaatca atgaaagtgg ttttaccgaa taataatgaa ctgacaatga
     7981 gctcattacg tcaattctcc gagcaaaaac cacgctatca atttgatgaa gaaaataaaa
     8041 tattactcaa taatgagact ggcaaacgtt atcaagcaaa tgacgaaacc ggtttcttcc
     8101 aatcgattga tgaaaacggt cattggcaaa gcgaaacact tgaaccaggt tatactgtca
     8161 catcaggctt taataacttc attaaaattt ttaccgatga aggtattcaa aaaccttttg
     8221 tccaaatatt tatttggacg gtgatcttct ctttattaac cgttgtcttt accgttattc
     8281 tcggtatggt tcttgcctgt ttggtacaat gggaggcatt aaaaggtaaa gcagtctatc
     8341 gtgtcttatt gattttacct tatgccgtac catcatttat ttcgattctg atttttaaag
     8401 gactatttaa ccaaagtttt ggtgagatca atatgatttt aaaccaacta ttcggcatca
     8461 gccctgaatg gtttaacgat ccgctccttg ccaaagtgat gatcttaatt gtcaatacgt
     8521 ggttaggtta tccttatatg atgattttat gtatggggtt actcaaagct attccggctg
     8581 atttatatga agcctccgca atggacggtg cttccacttg gcaaaacttc agtaaaatca
     8641 ctttcccatt actgttaaaa ccacttactc cgttaatgat tgcttcgttt gcatttaact
     8701 ttaataactt tgtattaatt caactattaa caaacggtcg cccggatatg attggaacaa
     8761 ccactccagc cggttatacc gatttattag tcagctacac ctatcgtatt gcctttgaag
     8821 gaagtggtac gcaagatttc ggcttagcag ccgccatcgc aaccattatt ttcttattag
     8881 tcggtggctt ggcactctta aatatcaaag caaccaaaat ggaattataa aagcgggagg
     8941 gatttaacaa tcccgacccc aaactaactt taggagttat atatggcaat tgttcaatct
     9001 aaatctgtgc gttatcgggt atgggcaacc catttcatct taatctgttt ccttgcctta
     9061 attattttcc ctttattaat ggtgattggc atttcgttac gaccgggaaa tctggctatc
     9121 ggtgatatta ttccaagcca aatttcgtgg gaacattggc aagccgctct tggttttgat
     9181 gttacacacg ccgatggtac agtcacaccg ccgccattcc cggtgctacg ctggttatgg
     9241 aactccatca aagtggcaac aattacctct atcggcattg taaccttatc tacgacctgt
     9301 gcttatgcat ttgcccgaat gaaatttaaa ggcaaaaaaa ccattctaca aggtatgctg
     9361 attttccaaa tgttcccggc agtgctttcc ttagttgcct tatatgcgtt gtttgatcgt
     9421 cttgggcagt atgtaccgtt tcttggttta aacacacacg gtggtgttat ttttgcctac
     9481 ttaggcggaa ttgccttaca cgtttggaca atcaaaggct actttgaaac gattgatggt
     9541 tcattggaag aagccgcttc ccttgatggt gcaaccccgt ggcaagcgtt ccgattaatc
     9601 ttattaccgc tttcagtgcc gattttggcg gtagttttca ttctttcatt tattgccgcc
     9661 attaccgaag taccggttgc ctcactccta ttgcgtgatg taaacagcta caccctagcc
     9721 gttggtatgc aacaatacct ctatccgcaa aactatttat ggggtgactt cgcagcagcg
     9781 gcggtcttat ccgcaattcc gattacgctg gtcttcttat tggcacaacg ctggttaatc
     9841 ggtggtttaa ctgccggcgg cgtgaaaggc tagtttgcaa aaaattctct aaaaacgacc
     9901 gcttgtcgag ctgatactgc ggtcgtcaat ctttattagg gctgtctatg aaaaatgcgt
     9961 taccttatct ctgcttattc tatacctcaa gcctttttgc ccaatcttgg caacattccc
    10021 atttttctga ttttaacgat gatctcgaaa aacatttttt tcaatcccaa gttcaattgg
    10081 aaaaagggag ctatccgctt aggttcaaat ttggcgaaca atgttatcaa ccccaacaag
    10141 cgataaaatt aaatcaatcg gttgcgttag tcccttgtat aaatgaagct ccacaactgc
    10201 ggttgtttcg tcaaggcaac tatctggcac aaattgatct gcgaagcggc actccgacac
    10261 ccacaatcag cgtagaacag cagctacagc acaatgaagc gatgtttcaa agttgcccga
    10321 attgggataa aaaaccgatt gaaattgatg tttcttccac ttttgccgaa ggcgaatcgg
    10381 ttagcgattt ttattcaggc aacaccgcta tcgttaaaaa cggcaaagtc accttaatgc
    10441 ccaatgaaaa cgcaagcggt ctgattttgc tggaaaaaag caataccgaa aatacggcaa
    10501 cgtttgattg gaaaaatgcc accgtctatt ttgtgctgac cgaccgcttt tataacggcg
    10561 atccgaccaa tgaccacagc taccaccgcc aaaaagacgg aatgcaagaa atcggcacct
    10621 ttcacggtgg tgatttaaaa ggattaaccg aaaagctcga ttacctacaa caacttgggg
    10681 taaacgtatt gtggattagc tctccgttag aacaaatgca cggctgggtt ggcggcggtg
    10741 ataaggggga ttttccgcat tatggctatc acggttacta tcatttagat tggacgaaat
    10801 tagatgccaa tatgggtact gaagacgatt taagtcgctt tgttcaacaa gcccaccagc
    10861 gtggtattcg ggtgctattt gatgtggtga tgaaccatac cggctacgcg acgcttgccg
    10921 atatgcagga gtttaacttt ggcggatttt acttaacacc ggacgaaatt agccgcacat
    10981 taggcgaaaa atggacacac tggaagccga aatcaggaca aaattggcac agtttcaatg
    11041 atttcattcg ctttaacgat aatcaggctt ggcaaaattg gtggggaaaa gaatgggtta
    11101 gagctgatat tgccgattac gacagcccta aatttgatga tttaaaaatg tcgctcgctg
    11161 cccttcctga tctaaaaacc gagagcgaac aagcggtcaa attacctgaa ttttttgcaa
    11221 ataaaaatac caatgccaaa acattaccaa acgccaaagt gcgtgattat ttaattggtt
    11281 ggttatccga ttgggtcaga aaatatggca ttgacggttt tcgggttgat accgccaaac
    11341 acgttgaaaa atccacgtgg ctggtattaa aacaatcggc acaacaagcc ttaaacgaat
    11401 ggcaaaaagc caatccgaac gccggtttta acgatcgctt ttggatgaca ggagaagcgt
    11461 ggggacacgg cgtgttcaaa agtgactatt atcaaaacgg ttttgatgca atgatcaatt
    11521 tcgattttca agatcaggct aaacaagggt taaattgctt tgcccatatt gagccgattt
    11581 atgccgaaat gaatcgcaaa ttaagcgact tcaatgtgtt gagctattta tcttcacacg
    11641 atactcgatt atttttccat tcggatagtg aacaaaatat cgaaaaacac aaaaccgccg
    11701 caaatttgct gttgctttca cccggtgcag tgcaaatcta ttatggtgat gaaagtgggc
    11761 ggacatttgg tgcgaccggt tcagacctga tacaaggcac ccggtcggat atgaattggc
    11821 aagagttgca gcaagatcct cagaaacagg cattgcatca acattggcaa aaattgacac
    11881 aattccgcca acgccatccg gctattggcg caggtgtcca tcaactcatc aaaaatgata
    11941 gctactttgc atttaagcgt gtgttgaatg aagataaagt gatggtggtt tgggccggaa
    12001 attaaatcta ctttagcttc aagttttctc tcttctgggg aaaacttgaa gccggttcgg
    12061 ggaaacaagt tgcaaaaaat tactaaaaat agaccgcttg ttccccatga gcttgtgatc
    12121 aatgataccc catcaacatc agcagttctt tcgtatagct gattgcttcg ttacgattcg
    12181 tcacaccgat tttctgataa agatttcgga tatgggtttt aatcgtggtt gcagccactt
    12241 gcaattcatc tgaaatttgt tcattgctat atccggagta aatcaaaccc aatacttgcc
    12301 attcccgctg agtgagcgga ctaattttta agagttccgg cactttcgga tttttcaata
    12361 attgattcac aaagttttca tcaaaatggg caaatttttg gcgataaaat tgattgatgt
    12421 tgcgtaaaat aaactgtgct ttgtgcaata ccagttcatc tagcacatta agttgcaata
    12481 aatggcggat ctgctgtgcc attacatccc cttcaatcac aaacgcactg ataaaattcg
    12541 tttggcgggt gagttttagt gcctgaatta aatcttgctg agccaattct ttctcgcctt
    12601 gtaaaaaata aagccggtta cgaacaatta atgcgcgatt taaatcgcta atcagagaaa
    12661 atttttcagc cgtttcaatt aaattatcta aaatggcttt cgcctcgtga tattcctcaa
    12721 gcaagattcg agaccgtgca atatttcgcc attgcacttg ggtaaaatga tttttatctg
    12781 aaaccggtga ggggttttga attaaccagt tcctcaccgc agaaacgtca ttggtcatct
    12841 gccaataaaa tatacgtacc tgatcggcgt tggcaatcca atcgtggtga taagaatgag
    12901 aatgttctaa ttgttcgacc tcgcctaata atcgggcagt gttatccaaa ttgcctcgag
    12961 ccaacgagat cttcgtgagt aaagttaagc attgtaactt atcttcaaat ttagataagg
    13021 cattcattcc ggcaactgcc attgattcag ctttatctaa gttataccat tcccataaaa
    13081 tcttgccttt agaacgtagc aaaaattcat acatcggcac tttttgtaga tggttttctt
    13141 tcacaaactc gcttgcttta tctaacattt cataggctgc ttgtgaaaac ccttgggcaa
    13201 gcaaaatttc agattgttga agctgtgacc acaaaatatt gtggtaagta tgatgctgac
    13261 gtgccatacg ctctgctttt tgcaacattg ctaatgcttc tgccagattg ccgtggcaat
    13321 gatgagcttc gccaataata gacgttgcaa caatatgagc ataataggca ttttctgaaa
    13381 gatcgtttaa cgcttcagac gctaattgaa gtgcggtatt ctcatcaccg gaattgatag
    13441 caacttgtgc ccgtagcaca ttaaattccg cattggcagt tttgctcaat tcaattttat
    13501 tttcagttaa tgcacgagaa aaatccgcta aaataccacc tacttcaata tggcggtgct
    13561 ggctttgtac caaccaggct ttgagtaaca ccaaattagt atgttcggtt aaatgggcgt
    13621 aatctaagca atttaagctc tcttccaata atttgagttc tccttgatga aacaccgtcc
    13681 aagcgtgttt gctcaaaata ttgagtagta atccggtatc cgataactgc attgcgtgat
    13741 gtaaggcttc tgtaacatag cctaattgta accacgcatt cgccgccttt tgatgcagtt
    13801 gcggtaattc agcatataac tcattttggc aacataaatt taaaaaggaa gcaaaaagcg
    13861 gatggaattt ccaccagcta tcatcactac tctgccactt actgtttgcc atttgttgta
    13921 aaaacaaacc ttgcttttcg agagactcta atttggtgcg actgttttgc tcccctgtaa
    13981 cagcctgcac taaagactca ttcattgaat gcaaaaccga acaacgcaaa ataaacaaac
    14041 gagtctcttc atcgacttta ttaagcactt catcggctaa atattcgtta atatgaaaat
    14101 ggtttgattt ggctaaacgt ttggcaatat cttgcagtgg tacttgtagc gtgtgtgatt
    14161 tattttttgc aaaaaggcta attagctgaa gggctgtggg ccacccttca acttcgttac
    14221 acagtgcgat aacatcttgc tgatttaact ccgaacctaa tcttgcttga aaaaaagcgg
    14281 tactttcttg gtgatcaaac attaactggt taatatcaat ttccaataat tgttcttgca
    14341 ctcttaaact ggcaatactt aacgggggaa ttgatcgaga aatcaagata agcgtcatat
    14401 tagacggttg atgtttaatc caatatttta atgcttcgtg aatttcatca ttttcaataa
    14461 tatgataatc atcaatagca agataaaaat gctctgaaaa tgccgcggct ttaatcaata
    14521 attgattaaa taaagctaat aaattagctt tacgattttc ctctaacaat acgtctattt
    14581 cttcattgat tgctgaatgc aatgccgcac taaaataagt ggcaaaacga tctgaattat
    14641 tatcgccttc atctaacgca taccagccga tattcttttt attttcaatc cattgggaaa
    14701 ccagagtggt tttgccataa cctgccggtg catttatcaa tacgaccgga taactgcaag
    14761 atttgtctaa ttcttgaatt aaacgttttc gaggaatgct gttttgtaat cgaaaagaac
    14821 aaacaagttt tgatggtatt aacataataa attcaatttt ctctcataac aaaataggga
    14881 tgatatatcc accatttgtg ttctcactac ttccttataa tcataccata caagatgatt
    14941 attgggatga tttgatttta tttggatttt acataatagc gacattcatc tttaaccggg
    15001 ggtttcctat gacatttaat tctattgtag aaaaatactg tcgctacttt gatgtgacag
    15061 atccaaaaca ctttacctta cagcagtggt atcaagttgt tgctgaaggt tcccttgagc
    15121 taatttatag ccagcctgca accaaaccga cagaatcacg ccacgtgaac tacctctcaa
    15181 tggagttctt aatcggtcgg ttaaccggca ataatttaat gaatttaggt tattacgaac
    15241 aaattcgtga ctatcttaaa caatatcaag ttgaattagt agatgtttta gaacaagagc
    15301 gtgatccggc attgggtaat ggcggattag gtcgtcttgc tgcctgcttt ttagattcga
    15361 tggcaactgt cggacaaaat gcaaccggct atgggcttca ttatcaatat ggtctgttta
    15421 aacaaagctt caaagaggga atgcagaaag aagcagcgga tacttgggct cgtgacagct
    15481 acccgtggca tcgttttaat ccgtctaaaa cccgccatat cgggtttggt ggaaaaatca
    15541 aacatatcca agccgatcaa tatgaatggc agcctaaatt gaccattcaa ggcaaagcct
    15601 ttgacttgcc gattgtaggt tataaaaaca acatcattca accattacgt ttatggcaag
    15661 cagatagtga ccaatctttt gattttgatg cctttaatga gggtaaattt ttaagtgccg
    15721 ataaaacgat tgtcaatgca gccgcactaa ctcaagtgct gtatccaaac gacaaccatc
    15781 aagccggaca aaaattacgc ttaatgcagc aatacttcca ttgtgcctgc tcggttgccg
    15841 atattttaga tcgtcacatt agcgaaggtt attcgttaga agatttagca aaacgccaag
    15901 ttattcaact caatgatacc catccaacgc tcgctatacc tgaattgatg cgtgtactgt
    15961 tagatgaaca tcaattcact tgggagcagg cttgggatat ttgcagcaat acctttgctt
    16021 acactaacca taccttatta cctgaagcac ttgaacaatg ggatcaacgc ctatttaagc
    16081 agctattacc tcgccattat caaattgtgg aaaaaatcaa tgatattttc cacgacaaag
    16141 tacaggcaga atttggtgat gatgcaaaaa tttgggaaaa attagccgtg ctatttgatt
    16201 accgtgtgcg tatggcaaac ctctgcgttg tcacctgctt ccgtgtaaac ggcgttgcgg
    16261 aaattcactc cgatctctta gtgaccgatc tattcccgga ataccacaaa ttattcccaa
    16321 caaaattctg caatgttact aacggcatca cacctcgccg ctggattcga caagctaatc
    16381 cacaattaag tgatttattg gatcgtacct taaaacaaga ttggacacac aatctggaat
    16441 tattaaaagg cgttgaacaa tatgcggatg acaccggttt ccgtgaagaa tatcaacgca
    16501 ttaaacgcaa taacaaagtg gttttagcca atgaaatcca taaaacatta gggttaaatg
    16561 tcaatcctaa tgcgattttt gatgtgcaaa ttaagcgttt ccacgaatat aaacgccaac
    16621 atttgaattt gttaaacatt attgcgacct atcaatcgct aaaagctaac ccgaaccaag
    16681 actacacacc acgtctattc ctattctcgg gcaaagctgc accgggttat tatttagcaa
    16741 aaaatattat tcacgctatt aataatgtgg cagatattat taataacgac aagcaggtaa
    16801 atgatcgact acaagtggca ttcttaccgg attatcgagt cagtcttgcg gaaaaaatta
    16861 ttccagcagc tgatgtatca gaacagattt caatggcagg taaagaagcc tccggtaccg
    16921 gtaatatgaa attggcactc aacggcgcat taaccttagg tacgctagac ggtgccaatg
    16981 ttgaaattgc tgatatggtc ggtgaggaga atgtctttat cttcggtcac accgtagaaa
    17041 gcgtgaggga attattagca aaaggctacc aaccgaaagc ctactataac caagatccga
    17101 ttctgaaaaa tgcggttgat ttcttagcca caggaaaagc ctctaacgga gataaagagg
    17161 cattcaagct gatgttagat agcctgttgg aacgagatcc tttcttagtc tttgccgact
    17221 ttgatagcta tcgccaagca caagagaaaa ttggtgtagc ctatttagat caagaaactt
    17281 ggctgcgtag tgcaatttta aatactgctc gtttgggtgc atttagctca gaccgctcaa
    17341 ttaaagatta tcaacaacgt atttggttga atagataata aattcgggcg acttctacaa
    17401 taaaggaagt cgcctatttt ctgctgtatt tcatatgtat ttatatatgg agagaaatga
    17461 tgaaaataac tgccaaacaa tattatcggg ctggaattat gccctatttt ttcgatgagc
    17521 gtggtgtaaa aaaaacagca tctgaaaaaa taaaacattc gttattcaaa gcctttaacg
    17581 gcaatactca agcaagagaa acacctatcc ctacggtcaa cattttttac cagcaccaac
    17641 cctgtttttt accttttcat ttagcggata aaaaacatcc gttaaccggt acttggcagc
    17701 tacaactaga aaattcaaac caaattattt ccggcaaaat taaagcaagg ggaattacat
    17761 tacctcaaga tttgccctta ggttatcacc aattacagct aaaaacggta gataaaacct
    17821 ttaattgcac cattattatc gctccggaac gctgctacca accacaagcc gtcattgaac
    17881 aaaaaagatt gtggggaacc ttcctacaac tatacacctt aaaatcagcc cacaactggg
    17941 ggattggcga ttttggcgat ttaaaagact tcattcaaca gattgcccct taccaagcag
    18001 attttatcgg cttgaacccg attcactcac tttttccggc aaacccgaat tctgccagcc
    18061 catacagtcc atcctctcgc cgatggctga atattgctta cattgatatc aatcagctag
    18121 cggaatttca acaaagtgat gaagcacaaa cttggtttca ttctgctgaa atccaacaac
    18181 aattatcaga attacgcact caggattggt tgaattacga gcaaatcatt ccgttgaaat
    18241 tgaaagggct acgctttgct ttcgcaaaat ttcagcaaaa tatgaccgct tgcgctgccc
    18301 ctgcttttat cgaatttgta caaaaaggtg gtgaaagctt acaagttcaa gccacatttg
    18361 atgcccttca tcagcatcta tcggcacaat ttgccgacca atggggctgg gatttctggg
    18421 agaaagccta ccaagactac cactccgcag ccgtgcagca atttagagaa acgcataaaa
    18481 ccgacataga attttacctt tggttacaat tcgttgccga tcaacagcta gcagaatgtg
    18541 atgctatctg ccgtcagcaa aatatgcgta ttggtatgta tcgcgatctc gccgttggcg
    18601 tcagcccgaa tggtgcagaa acgtggaatg acaaacaact ttattgctta aatgcctctg
    18661 tcggcgctcc gcctgatgta ttaggtccgc aaggacaaaa ctggggctta accccaatga
    18721 atccgcatgt attaaagcaa caagcctacc gtccgtttat tgagttagtc agagctaata
    18781 tgagtcattg cggtgcatta cgccttgacc atattatgtc tttactccgt ttatggtgga
    18841 tccccaaagg cgatagtgcg gttaatggtg cttatattcg ttatccagtt gatgatttaa
    18901 tcgcaatttt ggcacttgaa agccaacgcc accaatgcct aattatcggt gaagatttag
    18961 gtactgtgcc aaaagaaatt gtgagcaagc tcaaaaatgc ggggatttta tcttacaaaa
    19021 ttttctattt tgaatttgat gaacaaggga agagtcgaga cttacagcat tacccttacc
    19081 aagcaatgac cacattaagc acccacgatt taccgactat caatggttat tggcgtggct
    19141 atgattttga attaggcgaa aaattcggcg tctatcctaa ttcgaatatt ttggcaatcc
    19201 ttaagcaaga tcgagtcaat gccaaagcca aaatcttaca acgcttacaa gaacataacg
    19261 tagagatcgc acctgaaatt agcgagcaac ttaattctcc ggtgaatacc gcatttattc
    19321 actgtttaca aacctatgtt gcaaatgtaa gcagcggatt attcggcttc caacccgaag
    19381 actggttggg tatgactgag ccggttaaca ttccgggaac cagtacacaa tatgcaaact
    19441 ggcgacgtcg gcttagtgtt aacctcgagg agatcttcac agataaagat attcaagcct
    19501 tattaaaaga agttaattta atccgtaaaa atcaataaaa gttagataag aaaaaaggcg
    19561 actttccgtc gccttttggg cttatatgct tgatgtctat ctttatccac ctgaaaccca
    19621 caacataatc gctagcactt gaggtagcat aatacgcaaa aacattacta gtgggtaaac
    19681 agttgcatag gataacgctg cagcgccatt tccctcttta atttcattgg caaaagcaag
    19741 cgctggagga tcagtcattg agccggcaag taatccacaa atcgttaaat agtttacttt
    19801 gttatacatt ctggcaatca atcctgctat catcaatgga acaaaagtaa taatcgcccc
    19861 atagcatatc cactcaacgc cctcactgct taatagcgtt tcgagaaatc taccgccaga
    19921 ttttaaacca actaccgcta aaaacagtac aataccgatt tcacgcaatg caagattggc
    19981 acttggtggc ataaaccagt agagtttact gaaactacca attcttgcaa gaataagagc
    20041 aaccaccagt ggtccaccag ccaagcccag ctttaacgca acagggaaac ccggtaaata
    20101 aatcggtaca gaaccaagta atactcccaa accgatcccg ataaatattg gcaacatttg
    20161 gacttgttgc aactttgagt gcgcattgcc gataatcgtc attaccattt caatatcagc
    20221 ttgccgccct actaaattga gaacatcacc aaattgtaac gtcatatcac ctgttggtac
    20281 taattctaca ccggctcgat ttaaacgaga aatgacaaca tcgtatttgc ctttaagcat
    20341 tagctgacga atttgtttcc cgaagacttt ttcattcgtc actacagcac gttcagaacg
    20401 gtaaatcgtg cctttggttg agacagatac atctactgcc tcacctaaaa taatctgcat
    20461 ttttcgtaat gaggattctt cacctactaa gtgtaaaata tctcctatct gtagaatcgt
    20521 atctacacgt ggaatagaaa gctcattccc ccgttttaag cgtgaataag ctacttcgtg
    20581 caaattaaaa tcaggaattt gtttcaacgt taaaccgtga atatttggat tagtcagacg
    20641 gacatttaag gtactaagtt gatctttact tctattttgc tgtttgttaa aagcctctgc
    20701 ttccgcctca atattgattc gaaataaaat ccgaatcagc cacattgaaa gtagaattcc
    20761 gataatgccg aacgggtaag caatcgcata actcattccc ataatttcag ttacactctc
    20821 agcgcttaac tcagccaata cctgttgacc tgctcctaaa gaaggggtat tggttacagc
    20881 cccggagaaa atgcctaaaa tcacaggcaa aggaacattg aagaatttat gaataagaat
    20941 aactaaaata ccgctaatca gtacaatcag taccgcaaaa ccattgagtt ttaagccaga
    21001 atgttttagt gaggcaaaga atccaggtcc tacttgaatt ccaattgaat aaacaaataa
    21061 aatcagcccg aactcttgaa taaaatgaag cgtatgggca tcgagcttca catcgaattg
    21121 cgccataaaa tgggagacaa acagcccacc aaataacact ccccctatac ctagccccac
    21181 gcctctgatt ttaatatggc caatccataa gcctagaacg gccactaaag ataataaact
    21241 gacagtaagt gcaatttcgc tcattacagc ctctctttaa tatgaaatac ctcaaaaatg
    21301 gtatccaatt ctaccaaatc tcaaaaacga aaaatgag
//
LOCUS       DANLUA010000017        21266 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLUA010000017 DANLUA010000000
VERSION     DANLUA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..167)
                     /locus_tag="PXB44_000524"
     CDS             complement(<1..167)
                     /locus_tag="PXB44_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5281036.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
     gene            complement(373..2034)
                     /locus_tag="PXB44_000525"
     CDS             complement(373..2034)
                     /locus_tag="PXB44_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5281037.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            complement(2047..3516)
                     /locus_tag="PXB44_000526"
     CDS             complement(2047..3516)
                     /locus_tag="PXB44_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5281038.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            complement(3564..5915)
                     /locus_tag="PXB44_000527"
     CDS             complement(3564..5915)
                     /locus_tag="PXB44_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5281039.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXB44_000528"
     CDS             complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXB44_000528"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5281040.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            complement(7045..7383)
                     /locus_tag="PXB44_000529"
     CDS             complement(7045..7383)
                     /locus_tag="PXB44_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL5281041.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            7676..8995
                     /locus_tag="PXB44_000530"
     CDS             7676..8995
                     /locus_tag="PXB44_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL5281042.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            9113..9475
                     /locus_tag="PXB44_000531"
     CDS             9113..9475
                     /locus_tag="PXB44_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL5281043.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            9586..10623
                     /gene="waaF"
                     /locus_tag="PXB44_000532"
     CDS             9586..10623
                     /gene="waaF"
                     /locus_tag="PXB44_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL5281044.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            10690..11649
                     /gene="rfaC"
                     /locus_tag="PXB44_000533"
     CDS             10690..11649
                     /gene="rfaC"
                     /locus_tag="PXB44_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL5281045.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVECQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            11770..12144
                     /gene="rpsL"
                     /locus_tag="PXB44_000534"
     CDS             11770..12144
                     /gene="rpsL"
                     /locus_tag="PXB44_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL5281046.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            12275..12745
                     /gene="rpsG"
                     /locus_tag="PXB44_000535"
     CDS             12275..12745
                     /gene="rpsG"
                     /locus_tag="PXB44_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL5281047.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            12847..14949
                     /gene="fusA"
                     /locus_tag="PXB44_000536"
     CDS             12847..14949
                     /gene="fusA"
                     /locus_tag="PXB44_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL5281048.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            15009..16193
                     /gene="tuf"
                     /locus_tag="PXB44_000537"
     CDS             15009..16193
                     /gene="tuf"
                     /locus_tag="PXB44_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL5281049.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            16490..17788
                     /gene="tig"
                     /locus_tag="PXB44_000538"
     CDS             16490..17788
                     /gene="tig"
                     /locus_tag="PXB44_000538"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL5281050.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            17904..18356
                     /locus_tag="PXB44_000539"
     CDS             17904..18356
                     /locus_tag="PXB44_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5281051.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            18377..18877
                     /gene="secB"
                     /locus_tag="PXB44_000540"
     CDS             18377..18877
                     /gene="secB"
                     /locus_tag="PXB44_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL5281052.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            18984..19994
                     /gene="gpsA"
                     /locus_tag="PXB44_000541"
     CDS             18984..19994
                     /gene="gpsA"
                     /locus_tag="PXB44_000541"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL5281053.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            20010..20825
                     /gene="cysE"
                     /locus_tag="PXB44_000542"
     CDS             20010..20825
                     /gene="cysE"
                     /locus_tag="PXB44_000542"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL5281054.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            20874..>21266
                     /locus_tag="PXB44_000543"
     CDS             20874..>21266
                     /locus_tag="PXB44_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5281055.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVKP"
BASE COUNT         6422 a         4080 c         4572 g         6192 t
ORIGIN      
        1 gcagtttttt tgtttttttt gatctttcct tctgcaatca agcggtcttt ttctgcttgg
       61 attttgcaaa ggagttcgct tgccggttca tcatttggat cttgtggaac gagcttgcct
      121 tggattgcta gttgtaggat tgcgtttttt agctgttggg ctttcatttt ttattctcaa
      181 taatcggttg ttatttctta aagtatcttt gattacggtg ttttgggttt tctgggaatt
      241 gcatttcgat cacaccaagc gttagtgcag gttttaaata ctgcatacgg aaatgcttat
      301 catcttttaa cctgagtttt ttcataattt ctttgctctt catcggctca agcaacactg
      361 aaagtaagcg atttacttcg ggggtaattt gtttgctttg ttctggggta attgtttgac
      421 ttgcttcact ttgcttttct tcgggggggc gcccttactc tcactagctt tatcaagttg
      481 gtcgaaaggt ggattaaaat aatcatcttg tgatttctct gtgattatcc attctaaatt
      541 cactagggta tagagttgtt ttctcggact ccccgaactg gttagtaagc ctttttcttt
      601 cagtcgtgct agtgccaaag taatatttct gcgtggatat tggggcagtt tttgtgctaa
      661 ttctgcaaac attgctttac cttgattgaa ggcgataaag agtaagcaat ctttttgaat
      721 aggctgtaac tcagagaaat tttcgccaaa gcaagcggta aatttttcct caaaatctgc
      781 aatcatacct gattgaaaag ttaaggtgag tttagtgtaa tcaatttcac tttctaaaag
      841 cggtgaattg aaatttcggc tttgccaata tgaataaata tcccgaacgc cttgtccttg
      901 tcgctcacat agccctaaac ggtggaataa gttatgaata atagaattac ggcattccga
      961 tctttgccct gcaatagccg tgggaatatc aaccaacatt actcctgcat tttcaaaaat
     1021 cagtttgtta tctttggttt taatgacttt tagatggcga acattgttaa agtaatctgc
     1081 gtgagtaatt gcattgatta gagcttcccg tagcccgtca gtaatttcat tttcctcagt
     1141 acgggttaaa ttatctaatt tgaaatgttt atctttacca agctcttgaa taagtggcac
     1201 aattttcaag taaaattcaa agagattgcc ttcttcaaga tcaaaatcgg taatccgtaa
     1261 tgtatagcgt tttacatcac cagcttcttc gtaatattca aaaaagaagt ggggaaatat
     1321 gcttgtaatg gcatagtgct taccaaacat caataagcct gcattggtaa tgccctcgct
     1381 attctgtgct aaatcttttt tataagcccc aatttcttgc agcaatttct catcggataa
     1441 cgtaagtaat tttgatgtgc tgttatgggc tttcagcaga tttctgtatt tatccaatgt
     1501 gcttaaactg atatcatcaa tagaagcata aggcacaatt ctcccatcag catctttttg
     1561 gctatagctt gaaatgaaag aacgcagttc agaggaggtg agtttatgat cgcctgaatt
     1621 taaacgaaca taagaaagtc gataatcacc atttaaatgg ataggacggt ctaaaatatc
     1681 agctgctttc acataaattg caatcacgct tttattttca acttgaaaga ttttcacatc
     1741 attatccttt aataaggatt tactgacttt ttcactacga gattgggaaa ataattcatc
     1801 aatgacttta tcaggatcat taacaccaga aattagcagt tcaggaaaat gtccttgttc
     1861 attttccttt attcctaata aaataaagcc accattgctg tttgcaaaag ccgaataagt
     1921 atgccaaaga tcttttggca cactattttt tgcctcttta tattcaattt ctgccttttc
     1981 tcggataaat tgctgatgtg caatcatttt ttcgatatgt tcccaagtca gcattttatg
     2041 ctcctattac agtttaatgc ctaaaatatc ggtaatttct gctagaatct tatcaatatc
     2101 agcattgtaa gcggtgcgtt ttcgctgata atctgcaatt agttcatcag gcggaaggat
     2161 ttcctcttct tcgtgaggaa agccacataa atcgaggttg tattgacggt cggacatttc
     2221 ctttaatgta aaacttttcg ctttgtcaaa cccttcgact tcaatcactt ggcgatttcc
     2281 ccaccattca acaacttcgt tgaaatgttc caatttcatc ggtttagttt tggagaaatt
     2341 tttataaccc tgtggcatat caaggcgata aacccaagtt tcacgggttg gctcggtttt
     2401 atcaaaaaac agaatattag tcgtaatcga ggtataaggg gcaaagacac tatgcggtaa
     2461 gcggataatc gtatgcagat taaactcttc taatagcttt ttcttaatcg caactttggc
     2521 attatctgtg ccgaacaaaa atccgtcagg cagcaccacc gcagcccgtc cattctgttt
     2581 taggcggtac ataattaccg acataaacag atcagcggtt tcactgctct gtaaagccgt
     2641 agggaaattc tttttaattt gctcaatttc tgaacctccg taaggtggat tcatcaaaat
     2701 cacatcaaat ttatcatttt cggtgtaatc tttgaccgat ttttctagag cattatcgtg
     2761 atgtacattc ggattatcaa tatcgtgcaa caataaattg gtaatacaaa gcaggtgagg
     2821 aagggctttt ttctcaatgc catacacaga attattgaat aaagtgcggt cagaaagggt
     2881 ttgaatttga ctttccaata cctttaacgc cgaagttaaa aaaccacccg tcccacaggc
     2941 aaaatcggca atgcgttcgc caagtttcgg tttgattgct tgtaccataa aatcggttac
     3001 agcgcgaggg gtataaaatt cccctgcatt acctgcactt tgtaaacttt tgaggatatt
     3061 ttcataaata tcgccaaagg cgtgtcgttc ttggtaatgg gcaaaatcaa tttcatcaat
     3121 gatattgata acctgacgta ataacacccc atttttcata tagttgttat tatcttcaaa
     3181 agcagcacgg ataatccgtt ggttcatcgg cgtatcggct gaaataggca gattttttaa
     3241 agtcgggaaa agttcgttat tgacgaaaga gagtaattca tcaccagtca tcgctttgcc
     3301 gtctttttta tcttccgccc aattttgcca gcgtaaaaaa ttcggcaaaa ttgagtgata
     3361 ttcatcgttt tccagttccc actcttgttc tttagcatcg taaattttca agaataagat
     3421 ccagacgatc tgttcaatgc gttgggcatc accgttaatc cctgcatcgt tacgcattac
     3481 atcttgcagg cgttttacta tgttgttgag gctcattgtg atccttgttt atctgtaaaa
     3541 ttttgttgta aaataaccgc ttgttattcc gaataaatca tcttttctaa gccttttacc
     3601 gctttctggt agagttcttt accgccgaaa tttttaataa tagacactag acctccaaaa
     3661 cggctgaatt gcggtgtctt taaaatttcg gttttagtca gatcttgatt gccggatttg
     3721 acatattcat taagcagcaa ccctaaaact tgctggtttt cgggcgaata ctgctccaca
     3781 tagccacttt gtttcacttt ttctgctcgt tcggttttag tcagtggttt tttgccaaag
     3841 gagatgtggc ttaatacatc aaagtcttca acatcgtggc tgaattggcg tgtttggcga
     3901 agggctgcca gccattttgg atcggtgtaa aatttcgaga aaatttcacc gcttgttgtc
     3961 cattgctgtt ggaactgttc gtaactgccg aactcttttt gcatttcccg ttttgagaac
     4021 agatcaatat cttcagaaac cagctcgccc tgctcattca gatattggac tttctcgtct
     4081 aaaatgcgta atttgagtgt gccgaaatcg acaactaccg ggattttata tggctttagc
     4141 tcggggtctt taatgatgtc cttatcactg ggtttaggaa gcggttgttc gacaagtggc
     4201 ttattttccg gtacatctaa aatcaccacc ggctcaccat caaatgccgg atcagcaaac
     4261 aggcgagtaa cgcctcgaaa atctaaaatg gtgaaaaatt ctttgccgaa atcagggcga
     4321 agacgagtgc ctcgtccgat aatttgttta aattccgtca ttgattgaat gtttgcatca
     4381 agtacaacca gcttacaggt tttgctatct acacctgttg tcatcaattt agaggttacg
     4441 gcaatcacag ggtaatcttt cccttcattt gctacatcac aaaattcttc taaatcaacg
     4501 cctttgcctg attggtcgcc tgtaatttgg cagacatatt tggaattgtg tttcaacata
     4561 tcggcattga ggcgtgaaag ctctgccacc attcgctctg catggttgat gttggcacag
     4621 aagactattg ttttctgcaa gcggtcggtt ttgcgtaaaa attcgcaaat tctctccgca
     4681 accaagcggg tgcgatcatt tagcacaatg ttgtaatcga aatctttgac tgtatagagg
     4741 cgatcttcaa tctcattacc ttgatgatct tttaaacctt tggttggtcg ccagcctttt
     4801 aaatcacagt ccagatcaac acgaatcact cgataagggg caagaaagcc gtcttcaata
     4861 ccttgtttga ggctataggt ataaatcggc tcgccaaaat aggtaatgtt gctggcatca
     4921 tcggtttctt tgggggttgc agtcataccg atatgggtgg cgtccttgaa atacttcaaa
     4981 atagcacgcc agtcagaatc ttcttttgct gaaccacgat gacattcatc gatcacaata
     5041 agatcaaaga aagagggttg gaactgctta aacagttcgt tatcggtttc accgtcaagc
     5101 tgttgataaa gtgcaagata gacttcataa gcactgtcga gctttttatt gctgactttc
     5161 accattttgt ctttaaacgg tttgaagtct tgcatcattg gctgatcgac tagtacattg
     5221 cgatcggcaa gaaataaaat tttctttttc gcaccactgg tttttaaacg atggataatt
     5281 tggaaagccg tataggtttt acccgtgcct gttgccatta ccaataaaat cctctgctga
     5341 ccttttgcta cagcttcaac agtacggtta atcgcaattt cttggtagta acgaggctct
     5401 ttaaaggtgt cagtgctact ggtgtaataa ggctgttgaa taattttcag ctcttcggtg
     5461 gtatattgtt tcgcccaacg ttggtggagt tcgtcgggag taggaaatgc tgacatcgga
     5521 agggttcgtt gtacgccagt gataaaatcg tgttccacaa actcatcacc atttgagcta
     5581 tatgcaaaag ggacatcaag caatcgagcg tatgtcattg cttgctgtaa gccgtgtcgg
     5641 gaagagtgat ggttatcttt ggcctcgacc acggcaattt tttgatttgc ggaagtatag
     5701 agcagataat caacccgttt tgcttcgatg cgtttagttt gcgtcgtttt tggatcaaca
     5761 actacttgcc catctgtaaa tcgtttattg gttttaaatg tgtactcttc gccaatttgt
     5821 ctatccgacc atcctgcatt tctaattgca ggagagatta atcgagagcg aatttcttgc
     5881 tcgttcatct gctttaattc ttgaagtgtg tacatatttt tccttccgat aagagtttct
     5941 tatcggaaga ttatagcaaa cagtttaggt aaaattaact caggatttaa ttgataattc
     6001 tacccaactc ttcccccatt cgggtttgca ttcctgcaat aagtgcaggg ttgagatgta
     6061 ctttaccttt cgggaagagg ttaattacgg ttgagcctaa acggaaagct cccatttctt
     6121 cgcctttttg gagtttgatg gctttttcgc cttcggtttc gtaattccaa actaccactt
     6181 catcagcgcg aggcggatta attacgccag cccaaaccgt actcatactt gcggtaaccg
     6241 ttgcaccgac caaaatctgt agcattggtc cgaatgcggt gtcaaattca caaatcacac
     6301 gctcattgcg agcgaataag tttggaacgt gttcggctaa aaacggattg acggagaaca
     6361 attcgcccgg cacataaatc attttacgca atgttccatc gcacggcata tgtacacgat
     6421 ggtaatcagt tggcgataag taggtggtaa taaatgtgcc gtctttaaat tttgccgcca
     6481 tttcttcatc atttgccaat aaggtttcta gcgtaaaatg atgccctttt gcttgtaata
     6541 aacggttatc cgaaatttcg cctgattcgc tgactttacc ttctgccggt aagcaaagtg
     6601 tatttggatc ttgattaatc gggcgtgcgt tttcttttaa ttcacggata aaaaattcat
     6661 taaaagtagc ataatctgac ggctcggttt ttttagcttc agataaattg actttatact
     6721 gttttgcaaa cagtttgatg ataaggtgag taactacgcc ccatttttgc tctgctaacc
     6781 aacctgccgc acgggtaatt gccagttgtg gcagtaaata gtgcagagcg attttcacac
     6841 gttggaaata ggttggaact ggatattttt ttaattgcat tgcgttttcc ttttatttaa
     6901 aaacgagaat ttgcgtttaa ttgggggcga agtatagcaa atacaatgca aaatagataa
     6961 aaaatcttta aggtgttgaa gtggctacaa agaaaaaaac agataggggc tttgtaggaa
     7021 ttccacttca acaatttggt tgtcttattc taaaataata ttggctcgat ttttttctac
     7081 cgttgcttgc ccaattccgg agacttcagc aagttgctca atgtttttaa aattgccgtt
     7141 tttagtacgg taatcgacaa tcgcttgagc ttttttctgc ccaattccca ctagcttgtc
     7201 ttgcaattca gcagcactag cagtattgat atttaccgca ttagcgttgg tgatttgtgt
     7261 ttgctgtacg ggttgagtga ctgcattttg ttcaacaggc attgtagttg gcggtgtttg
     7321 ggcaaaagcc attgcattta agccaaacaa gaatgttaga ccaagttgtt tgattttctt
     7381 cataggattt ccttattagt tgttgaaagg tcgaaaggaa gattaagagg gaataaaata
     7441 gaactcaacc ggcaaaaata aatttttcga cacagatcga aaaaatagaa aatggctaaa
     7501 cttctagacg tgcatttggt ctgaaattca atgaaagtaa atagatttgc aaaaaattag
     7561 gcaaaaatga ccgctagttg cgtaaattta gttaaataaa ttcaaatagc ggctgaattg
     7621 ctttagaata tcgccatttt tatactcatt aacaacgcta ttatttggaa ttaatatgac
     7681 acaaaataat tttcgcaaag aaccggtttt tggtgaatct tcggttacct ctcaagacgc
     7741 tgaagcaacc aaaacagata cagctcaagc cccttatgtt tcgttgcgtt caaccaaagc
     7801 accggggcat acgtttaccc caattatgaa aagacctgat gctaaagtag aagatcatac
     7861 ggctgatgtc ttttcggcta agcaggcaaa agaattccaa tttactccaa gttcagaagc
     7921 tacagaagca gtagagccgg ataataccat tcattcggtt gaagaaagtg tatcaaccgc
     7981 atcttttaac gcggttgaag cacaagaaaa tgtggaaaaa gaagtggtta aacctgcacc
     8041 cgagagtttc actactgagc gtattattcc aaatgcggca acagctgccg cagcagccac
     8101 tgcggcagct gcagccacta aaaccaaaga gattaaagct aaaatgccac ctaaaacccg
     8161 ccgtttatta ttagtggcat tgttggcgtt agcattattg gtactgtttt tcttgttaaa
     8221 accaagtacg ccggaaactg tcgaagagct acaatctcag caaggtggta gcttgccgat
     8281 tgaattccga ccggttgatg aagctgaagc gaaacgtgcg gaagaacaag ctcgtgcaga
     8341 acaagaagcc gctgaagctg caaaagctca agaacaagct caagccgctc aacaagagtc
     8401 tgtaacagtt gctccagccc aaaccgagca aacaatggct aaaccgtcac aagatagcca
     8461 gtcgcaaaca caatcacaag cagtagttga gcaacctgtt gcggtgaaac cggtggaaaa
     8521 taaaccggtt gttgcacaac cggcgattaa gcctaaaacg gaaaatagcg tggtgttcca
     8581 acctgaaaca acggtaaaac aacagcctaa agcccctgct aaaactgaag tgaaaaccga
     8641 acgtgctaca ctggcagcaa aagaaacggc aaaaacagca gctaaaccag ttgagaaagc
     8701 tccgacaaaa actgaagcgg taaaagcagc accggctgcc gagtcggcaa atgcggtagc
     8761 aaccaaaaca atgactgtgc caaaaggtgt atcactaatg caagtgttcc gtgataacaa
     8821 tttaaatatc tcagacgtta atgcgatgag caaagtcaat aacgtggtga gtaatttaaa
     8881 agtaggcgag cgagtaaccg ttcgtttaga taaaaataac cgtgtggttg aaatgagcat
     8941 tggctcaggc ggcaaattta cccgtcaagc aaacggtagc tatactttta aataaataaa
     9001 atgaagaaaa agagcaacgc tacaaacgtt gctcttttat ttaaaaagga gataaaggat
     9061 gaatttattt tgcccctctt acaaacttat ctacttaacg ctaagctcag cgatgttagt
     9121 tgcttgtagt acagtggtta cccctgttga gcaaattcat caagtacaaa aggaaaaaga
     9181 gtcagtaaag aaagtgacta aactgatagc acaagcacct aaagtgagtt tatatcgttg
     9241 tgagaatgat aagaaggttg aagttgaacg ccagggacga acaaaaaaag cgttaaaaaa
     9301 ggaaatagtc acagtgagtt atcaagggac aacgcatcag ctttcacctt ctgtaacgag
     9361 agatggtaaa aaatacacta atatccgttg gacttggcat gaaacccgta gtggtaaagc
     9421 atttttgtat aacaacacta aaaaaacgtt agcggcaaat tgcataaagc agtaacaagc
     9481 ggacacaaaa agtatcgctt tttgaacgtt ggctttgcca acggcagcga agctgtgaac
     9541 aatccttaca aggattgtga ataaattttt aagagaattt tgcaaatgaa tattttagtt
     9601 ataggaccgt cttgggtggg tgatatgatg atgtcgcacg cactttatca acagcttaaa
     9661 atacagtatc cgaattgcca aattgatgta atggcacccg attggtgttg tccgctttta
     9721 gcccgtatgc ctgaggtgcg taatgcgatt tcaatgccga ttggacacgg ttcatttcgt
     9781 ttgtgtgaac gctaccgact cggaaaatcc ctgagaaatc aatatgatat ggcgattgta
     9841 ttgcctaatt cattaaagtc tgcctttatt ccactgtttg cgaaaattgc gagacgtcgt
     9901 ggttggaaag gggaaagtag atattttttc ttaaatgatc ttcgcagtaa taaaaacgac
     9961 tacccaatga tggtgcaacg ctatgtagcc ttggcgtttg aaaaggctac tgttccaacc
    10021 gcacaacagt tgccgattgc ttatccatac ctcacaactg atagcgaaca aattgagcag
    10081 accaaagcga aatttgaaaa acagttggct tatgcggaaa accgtcctgc catcggcttt
    10141 tgtccagggg ctgaatttgg accggcaaaa cgttggccgc attatcatta tgcaatattg
    10201 gcaaaaatgt tgattgagca aggctattcg gtacggttat ttggctcgaa aaaagatgaa
    10261 gccgccggtg agcaaattcg cttggcatta cctgaaaatt tacaacgcta ttgcctgaat
    10321 ttagccgggc aaaccgattt aaaccaagcg gtagatttga ttgcagattg taatgcagtc
    10381 gtgagcaatg actccggctt aatgcacatt gccgcagcac taaataaacc gctggtcgcc
    10441 ttatatggac cgaccagccc acaatatacg ccgccgttat cccaaaatgc cgagatcatg
    10501 cgcttaattg aaggcggttt gattaaaatc cgcaaaggcg aaggggcaga gggctatcac
    10561 caaagtttaa ttgatattca gcctgaaatg gtgatggaca aattagctga actgttagtt
    10621 taaatggata acaagcggtt gaatttgcca attttttgca aaaaaagacc gcttgtttgt
    10681 ttataaaaga tgaaaatttt aatcgttaaa acctcatcaa tgggagatgt attacatacg
    10741 cttccagcca taaccgatat gcaaaatgct attcccgatt taaaagtaga ttgggtggtg
    10801 gaggagaatt ttaccgaaat tcctgcgtgg cattcagcgg taaataaagt aattccggtg
    10861 gctattcgcc gctggcggaa aaacctttgc caaagacaga cttgggtaga atgccagcag
    10921 tttttaaaac agctaaaaag tgagcagtat gatgcggtaa ttgatgctca agggctgatt
    10981 aaaagtgcgg tgttagtaac tcgccaagcc aaaggcaaaa aatttggcta tgataaaaac
    11041 tctgcccgag aagggttaag tagtctgttt tacgatgaaa cgtttaatat cccctaccaa
    11101 caacacgctg tagaacgcat cagaaaatta tgtgcaaaat cattaggtta tgctttgcca
    11161 accgaacggg gcgattattg cattgcacaa cattttgcaa aaaatcctca aaaaacgacc
    11221 gcttacatta tgctgattca cgccactacc cgagcggata aacattggca agaagcctat
    11281 tgggcggagt tgattcaagc gttggataag cacaatctgg cagtcagatt gccttgggga
    11341 aaccaagccg aaaaagaacg ggcggagcgt ttagcgagtg tggcgaataa tgctaaggta
    11401 ttgccaaaaa tgagcttaac cgatattgct actcaacttg cgggagcagc ggcagtggtg
    11461 tcggtggata ccggcttgag ccatttggcg gctgcgttag ataagccaaa tgtgatttta
    11521 tatggggcaa ccgatccaaa acttatcggg gcttacgggc aaaatcagtt ttatctaaaa
    11581 gcagataaaa tggaaaatat tacctcttca caagtgctag aaaaattact tccattgatt
    11641 aacaaatgat cattgttgtt ctcttactgt gcttgacttt tatccacgtt caatttagaa
    11701 ttcaccgtcc tttcgtgtga aaggatgttt ttaattttat ttttaataat tttaactgga
    11761 gcttttttaa tggcaactat caaccagcta gtacgcaaac cgcgtgtgaa aaaggttgta
    11821 aaaagtaacg ttcctgcatt agaggcttgc ccgcagaaac gtggcgtgtg tactcgtgta
    11881 tacactacta cacctaaaaa accgaactca gcattacgta aagtatgtcg tattcgttta
    11941 acaaatggct ttgaagtaac ttcatacatc ggtggtgaag gtcacaacct tcaagaacac
    12001 agtgttgtat taatccgtgg tggtcgtgtt aaagacttac cgggtgttcg ttaccatact
    12061 gtacgtggtg cacttgactg tgcaggcgtt aaagatcgta aacaaggtcg ttctaaatac
    12121 ggcgttaaac gtcctaaagc ttaatggttc tccgttgagt aaggccaaac gtctaaattt
    12181 taatatttaa accataaact ccagtcaaga tgccttaagg cagattagat gagttttgga
    12241 tatcctgaag atttaatata cggagaaatt tccaatgcca cgtcgtcgta gtattgaacc
    12301 acgcaaaatt cttccagatc cgaagttcgg ttcagaatta cttgcaaaat ttattaacgt
    12361 gttaatggta gatggtaaaa aatctactgc agaaaaaatc gtttacggtg cattagaaac
    12421 tttaacacaa cgttctggta aagaagcgtt agaagctttc gaaatcgcat tagaaaacgt
    12481 acgtccaact gttgaggtta aatctcgccg tgtgggtggt tctacttacc aagttcctgt
    12541 agaagttcgc ccaacccgtc gtaatgcttt agcaatgcgt tggatcgtag aagccgctcg
    12601 taaacgtggt gacaaatcaa tggcattacg tttagctaac gaattatcag atgcagcaga
    12661 caacaaaggt tcagcagtga agaaacgtga agacgttcac cgtatggctg aagctaacaa
    12721 agcgtttgct cacttccgtt ggtaatcgtt taagcaaact tatcaaaggc ttcatctcag
    12781 tttgcggtga agccttaccc ttattaagaa ttttaattta aaccccaaac aaggtaataa
    12841 ataaaaatgg ctcgtacaac ccctatttca ctttaccgta acatcggtat cagtgcgcac
    12901 atcgatgcgg gtaaaacaac tactacagag cgtatcttgt tctatacagg tgtaagtcac
    12961 aaaatcggtg aagttcacga tggtgctgca acaatggact ggatggaaca agaacaagag
    13021 cgtggtatca cgatcacatc tgctgcaaca acagcattct ggtctggtat gtcacaacaa
    13081 tatccacaac accgtatcaa cgttattgat actccgggac acgttgactt tactatcgaa
    13141 gtagaacgtt caatgcgtgt acttgatggt gcggtgatgg tttattgtgc agttggtggt
    13201 gttcaaccac aatctgaaac tgtatggcgt caagctaaca aatataaagt gccacgtatc
    13261 gcgttcgtaa acaaaatgga ccgtacaggt gcaaacttct tacgtgttgt tgagcaaatc
    13321 aaaacgcgtt taggtggtaa ctcagttgca ttacaacttc caatcggtgc agaagacagc
    13381 ttcaaaggtg ttgttgattt aatcaaaatg aaagcaatca actggaacga agctgatcaa
    13441 ggtatgacct tcacttatga agatattccg gcagatatgc ttgaagcttg tgaagaacac
    13501 cgtgcaatgc ttgttgaagc agcagctgaa gcttctgaag aattaatgga aaaattcttc
    13561 tctggtgaag aattaactga agatgaaatc aaagcagcat tacgtcaacg tgtattagca
    13621 ggtgaagtaa tccctgtttg ctgtggttct gcatttaaaa acaaaggtgt acaagcaatg
    13681 cttgatgcgg taatcgatta cttaccggca ccaactgata ttcctgcaat taaaggtatc
    13741 aatgaagatg aaactgaagg tgagcgtcac gcaagcgacg atgagccatt tgcttcatta
    13801 gcattcaaaa ttgcaacaga cccattcgta ggtaacttaa ccttcttccg tgtttactct
    13861 ggtgtaatta actctggtga tacagtttac aactcagtaa aacaaaaacg tgaacgtttt
    13921 ggtcgtatcg tacagatgca cgcaaacaaa cgtgaagaga tcaaagaagt tcgtgcgggc
    13981 gatatcgctg cagcaatcgg tttaaaagat gttggtacag gtgacacatt atgtgcaatc
    14041 gatgcaccaa tcattcttga gcgtatggaa ttccctgagc cggtaatctc tgttgcggtt
    14101 gaaccaaaaa ccaaagctga ccaagagaaa atgggcttag ctttaggtcg tttagcacaa
    14161 gaagaccctt cattccgtgt tcacacagat gaagaatctg gtgaaacgat catttcaggt
    14221 atgggtgagt tacacttaga catcatcgtt gaccgtatga aacgtgagtt taaagtggaa
    14281 gcaaatatcg gtaaaccaca agtatcttac cgtgaaacta tccgtactcg tgttaatgat
    14341 gttgaaggta aacacgcaaa acaatctggt ggtcgtggtc aatatggtca cgttgtgatc
    14401 gacttatacc cattagatgc tgaaggtccg ggttatgagt ttgtaaacga gatcaaaggt
    14461 ggtgtaatcc ctggtgaata cattcctgcg gttgataaag gtatccaaga acagcttaaa
    14521 tctggtccgt tagcaggtta cccggtagta gatttaggtg tgcgtttaca cttcggttca
    14581 taccacgatg ttgactcatc tgaacttgcg tttaaacttg ctgcatcatt agcatttaaa
    14641 gcggcgttcg caaaagcaag cccggtatta cttgagccaa tcatgaaagt ggaagttgaa
    14701 actccaccgg attacgtggg tgatgtaatc ggtgacttaa gccgtcgtcg tgcaatggtt
    14761 aacggtcaag aagcgaacga gttcgttgtt aagatcaatg ctgaagttcc actttcagaa
    14821 atgtttggtt atgcaacaga cttacgttca caaactcaag gtcgtgcatc atactctatg
    14881 gaacctttaa aatatgctga agcaccgaaa aacgttgctg atgcaattat tgaagctcgt
    14941 agaaaataat ttgtttaacc tgagccatag caatagggct atggctcatt ttttaggaag
    15001 atattaaagt gtctaaagaa aaatttgaac gtacaaaacc gcacgttaat gtgggtacaa
    15061 tcggccacgt tgaccatggt aaaacaactt taacagcagc aatcactacc gtattagcaa
    15121 aacacttcgg tggtgcagct cgtgcatttg accaaatcga taacgcgcca gaagaaaaag
    15181 cgcgtggtat caccatcaac acttcacacg ttgagtacga tacagcaact cgtcactacg
    15241 cacacgttga ctgcccggga cacgcggact atgttaaaaa catgattacc ggtgcggcac
    15301 aaatggacgg cgctatctta gtagtagcag caactgacgg tccaatgcca caaactcgtg
    15361 agcacatctt attaggtcgc caagtaggtg taccatacat catcgtattc ttaaacaaat
    15421 gcgacatggt agatgacgaa gagttattag aattagttga gatggaagtg cgtgaacttc
    15481 tttcacaata cgacttccca ggtgatgaca ctccaatcgt acgtggttca gcgttacaag
    15541 cgttaaacgg cgtagcagag tgggaagaga aaatccttga attagcaaac cacttagaca
    15601 catacattcc agagcctgag cgtgcgattg ataaaccatt cttattacca atcgaagacg
    15661 tattctcaat ttcaggtcgt ggtacagtag taacaggtcg tgttgagcgt ggtatcatcc
    15721 gcactggtga tgaagttgaa atcgtaggta tcaaagatac agcgaaaaca accgtaacag
    15781 gtgttgagat gttccgtaaa ttattagacg aaggtcgtgc gggtgagaac gtaggtgcat
    15841 tattacgtgg tactaaacgt gaagaaatcg aacgtggtca agtattagcg aaaccgggtt
    15901 caatcactcc acacacagac ttcgaatcag aagtttacgt attatcaaaa gaagaaggtg
    15961 gtcgtcacac tccattcttc aaaggttacc gtccacagtt ctacttccgt acaacggacg
    16021 taacaggtac aatcgaatta ccggaaggtg tagagatggt aatgccgggc gacaacatca
    16081 aaatgactgt tagcctaatt cacccaatcg cgatggacga aggtttacgt tttgcgattc
    16141 gtgaaggtgg tcgtacagta ggtgcgggcg ttgtagctaa aatcatcaaa taattaagat
    16201 tgtttgataa attgaaaagc taaataaaaa ctagaaagag gcatatcgaa aggtatgcct
    16261 cttttgcttt tatgccgatt aagctagcca tatcatatca tttcatttgc aaaaatcggt
    16321 gaaaaaagac cgcttgtagg caagcccatt gacaagcagt cagattttag ggatttttta
    16381 caaaactgtt tatttgcgaa tgagtttctt ctcgacagtt tcttgataga acgctacaat
    16441 aaccgtctat ttgtatatct tttttatatc cctaaaccga ggtttaagaa tgtcattttc
    16501 aattgaaaca ttagaaggct taaaacgccg tgttactatt aacgtaccag ctgataaaat
    16561 cgaagcagca tacaaagaac aattaaaagg ctatgcgaaa aatgctcgtg tagatggctt
    16621 ccgtaaaggt aaagtgccac actcaattat cgaacaacgt tttggtattg cagcccgtca
    16681 agatgtgtta tctgatgaaa tgcaacgtgc attctttgaa gccgtcattg ctgaaaaaat
    16741 caacttggca ggtcgcccta ctttcacgcc aaacaactac caaccaggtc aagacttcag
    16801 ctttactgcg gaatttgaag tattcccaga agtggaatta aaaggtttag agaatatcga
    16861 agtagaaaaa ccggttgttg aaatcgcaga cgctgattta gacaaaatgg tagatgtatt
    16921 acgcaaacaa caagcgactt ggtcagaaag ccaagaggct gcaaaagccg aatctcgtgt
    16981 aactattgat ttcgttggtt cagtagatgg tgaagaattc gaaggcggta aagcatcaga
    17041 cttcgtgtta tttatggggc aaggtcgtat gatcccgggg tttgaagacg gaatcgtagg
    17101 tcgtaaagcg ggtgagcaat tcgacattga tgtgactttc ccagaagaat accacgctga
    17161 aaacttaaaa ggcaaagcag cgaaatttgc aattaccttg aaaaaagtag aagagatggt
    17221 attaccggaa ttaaccgaag agtttgtgaa aaaattcggt aatgcaaaat cagtggaaga
    17281 attacgcgca gaaattaaga aaaatatgca acgtgaactt aaaaatgcag tcacttcacg
    17341 tgtgaaaact caagtaatca acggcttaat tgctcaaaat gatgttgaag tgccggctgc
    17401 tgcggttgaa gaagaaatca acgtattacg ccaacaagcg gtgcaacgtt ttggtggtaa
    17461 gcctgaaatg gcagctcaat taccggctga attatttgaa gcagaagcta aacgccgtgt
    17521 tcaagtgggc ttattattat cttcaatcat cggttctaac gaattaaaag tagatgaaaa
    17581 acgtgttgaa gagatgatcg cagatatcgc ttcagcttac gaacaaccgg ctgaagtggt
    17641 tgctcactat gcgaaaaacc gtcaattaac tgaaaatatc cgcaatgtgg tattagaaga
    17701 gcaagcggtt gatgcagtgc tagcgaaagc gaaagtgact gaaaaaacaa tgtcatttga
    17761 cgaagtaatg tcacaacaag ctcaataatt tccttataag gtgggctttt gctcaccttt
    17821 tattactttt acaagcggtt aaatttagtg ataaatttgc atcaaaacga ccgcttattt
    17881 tttgatgtta aggaaattga attatggaac aactaacttt tgctcaacaa ttagagcaat
    17941 tttttgccaa tcataccttg atggtgtttg cttgggttgc attattgatt gcggtaattt
    18001 ttagctttta taaaagtgca acgcaaaaat ataaaattgt cgataacgca caggctacac
    18061 agctgattaa taaagaagag gcagtagtgg ttgatttacg ttctgatgaa gagttccgtg
    18121 ccggtcatat tattgatagc attcacgtgt taccgtcaga cattaaagca gctaaaatca
    18181 atcagattga taaatacaaa gctcgcccgg taattgtagt agacactaat gacttcactt
    18241 caggtggtgt tgcggcagat cttgctaaac aaggctttag cagagtttat gtattaaaag
    18301 aagggattgg cggctggcgt gcagcaagcc taccaactat taaaaaacat aaataatcta
    18361 ttattaagga aattttatgt cagaagaaaa tcaagttgca tcaacagaag aagctacaca
    18421 aacccctttt gagcttcaaa ttcaacgcat ttatgtaaaa gatgtttcat ttgaagcacc
    18481 aaatttaccg actattttcc aacaagagtg gaaaccacaa ttaggctttg agttagatac
    18541 tgaaaccaaa gaaattggtg aagatttata cgaagttacc ttacacatta cggtgcaaac
    18601 tacgttagaa gacagcaatg atgtcgcttt catttgtgaa gtaaaacaag caggtgtgtt
    18661 tactattaaa ggtttagaag gcattcaact tgctcactgc ttagcttcac aatgtccgaa
    18721 tgtgctttat ccttatgctc gtgagctaat ctcaagcctt gtgaatcgtg gtacattccc
    18781 ggcattaaat ctctcaccgg taaactttga tgcgttattt atggattatc tccaacgcca
    18841 agaagaaagt gcagaagagc cggctttagc gaactaagca ttttagatag aaaataggta
    18901 tgcaaggcat acctattttt ttacaagcgg ttattttttc tactattttt tgcaattttc
    18961 taaataagcc gaacagagga aatatgagtg aaatctattc tgccccaatc acggtattag
    19021 gtgcaggttc ttatggaaca gctttagcca ttgccctttc tcgaaatggt cacaaaacct
    19081 atttatgggg gcataatccc gacaaaatgg ccgttttggc atcagagcga atgaaccaag
    19141 attttctgcc tgatattgta ttcccggaag ccttagaaat tgtcgcagat cttgctacag
    19201 cggtacaaaa atcgaaagat ctgctgattg tggtgccaag tcatgtattc tctgaagtat
    19261 tggctcaaat caagcctttt ttgtcccaag atcaccgtat aatgtgggca accaaagggc
    19321 tggagcgaga taccgggcgt ttgttgcaaa cggtagctaa ggaaattttg ggggaaattt
    19381 acccgcttgc ggtgctttcc ggtccgactt ttgccaaaga actggcggca ggcttgccaa
    19441 cagcgatttc gcttgcctca actgatcctg tttttgccga tcaaatgcaa aaacggagtc
    19501 actgttccaa agcgttccga gtttacctca acaatgatat tatcggtgtg caactcggtg
    19561 gggctattaa aaatgtgatt gcaattggtg ccggtatttc agacggaatg ggattcgggg
    19621 cgaatgctcg taccgccttg attactcgag ggctggcgga gattagccgt ttgggtgttt
    19681 cgctcggggc tgatccgaaa acctttaccg gaatggcggg gcttggagat ttagtgctga
    19741 cgtgtaccga taaccaatcc cgaaaccgcc gatttggttt ggcgttaggg caaggaaaat
    19801 cggcagaagt cgcgatagcg gaaatcggtc aagtggtaga aggattttat aataccaaag
    19861 aagcctattt gctcgctcaa acacagggcg ttgaaatgcc gattgtagaa caaatttacc
    19921 aaatgttatt ttgtggcaaa caggcttcgg atgtggtgaa aaccttgttg gggcgtgaac
    19981 gaaaaggcga ataaagaaag gagaataaaa tgcagcagag tgaaattaat cagatttggc
    20041 aagcaattcg agatgaagcc aaagagctgg cgaattgtga acctatgtta gcaagttttt
    20101 tccacgctac cattttaaaa caccataatc taggcgatgc gttaagttat attttggcga
    20161 ataagttaga aaacccgatt atgccggcaa tcgccttaaa agaaattatt gaggaagctt
    20221 atcgagcaga gccacaaatt attgctagtg cagcttgtga tattaatgcg gtcagaacta
    20281 gagatcctgc ggtggacaaa tggagtacac ctctgctgta cttaaaaggc ttccacgctt
    20341 tacaaagcta tcgggtgacg cattatcttt ggaatcaagg caggaaagca ttagcggttt
    20401 atttacaaaa tgaaatttct gttgcctttg atgtggatat tcatcctgcg gcaaaggtcg
    20461 gttgcggaat tatgtttgac cacgccaccg gtattgtggt gggtgaaacc tctgtgatcg
    20521 aaaatgatgt ctcgatttta caaggtgtaa cactaggcgg tacagggaaa gaaaatggcg
    20581 accgccaccc gaaaatccgt gaaggcgtga tgattggagc cggtgcaaaa attctcggta
    20641 atattgaaat cggacgctat tctaaaatcg gggcaaattc agtggtgcta caacctgtgc
    20701 ctgatcacgc cacagcagca ggtgtaccgg ctcgcattat cggcaaatcc tctgaacaaa
    20761 aaccggcttt tgatatgaat caatactttg aagatgagca aggtttgttt ggtgatggga
    20821 tttaggtaag agttattcct ttaatttatg atttagacat tgctaagtac aagttgcaat
    20881 taacctttaa tgattttcag aactactata atggtaaagg ggatttaact atgttttctt
    20941 tttatggtaa gaaaacatgg gaaaatggca tttatgtagg gattgatggt agcattgggc
    21001 gtgcgaataa caaattaagc catcaaagcc agacgataaa actagccaga aatgtatatt
    21061 ctttaggatt aacggtaggt aaaacgttgg aagttgcaag ttttaaccta caaccgtcat
    21121 ttggtatgcg ctatcatcat ttaacatctg caaatgccca attagagaac acaaaatttg
    21181 agactgatag ggtagatctt ttggccattc aagctggttt agcattaaat aaaactttta
    21241 tatttaatga attaaaggta aaacct
//
LOCUS       DANLUA010000018        20707 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLUA010000018 DANLUA010000000
VERSION     DANLUA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            96..842
                     /locus_tag="PXB44_000544"
     CDS             96..842
                     /locus_tag="PXB44_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5281056.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            1038..2801
                     /gene="fucI"
                     /locus_tag="PXB44_000545"
     CDS             1038..2801
                     /gene="fucI"
                     /locus_tag="PXB44_000545"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL5281057.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLHFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRANETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            2995..4428
                     /gene="fucK"
                     /locus_tag="PXB44_000546"
     CDS             2995..4428
                     /gene="fucK"
                     /locus_tag="PXB44_000546"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL5281058.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            4503..4934
                     /gene="fucU"
                     /locus_tag="PXB44_000547"
     CDS             4503..4934
                     /gene="fucU"
                     /locus_tag="PXB44_000547"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL5281059.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            4994..5647
                     /locus_tag="PXB44_000548"
     CDS             4994..5647
                     /locus_tag="PXB44_000548"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL5281060.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            5709..7238
                     /locus_tag="PXB44_000549"
     CDS             5709..7238
                     /locus_tag="PXB44_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5281061.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            7235..8233
                     /locus_tag="PXB44_000550"
     CDS             7235..8233
                     /locus_tag="PXB44_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281062.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            8257..9204
                     /locus_tag="PXB44_000551"
     CDS             8257..9204
                     /locus_tag="PXB44_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5281063.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            9260..9511
                     /locus_tag="PXB44_000552"
     CDS             9260..9511
                     /locus_tag="PXB44_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5281064.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            9640..10791
                     /gene="fucO"
                     /locus_tag="PXB44_000553"
     CDS             9640..10791
                     /gene="fucO"
                     /locus_tag="PXB44_000553"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL5281065.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
     gene            11058..12503
                     /gene="aldA"
                     /locus_tag="PXB44_000554"
     CDS             11058..12503
                     /gene="aldA"
                     /locus_tag="PXB44_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL5281066.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            12759..13406
                     /locus_tag="PXB44_000555"
     CDS             12759..13406
                     /locus_tag="PXB44_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL5281067.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(13539..14915)
                     /gene="trkA"
                     /locus_tag="PXB44_000556"
     CDS             complement(13539..14915)
                     /gene="trkA"
                     /locus_tag="PXB44_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL5281068.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(14915..16246)
                     /gene="rsmB"
                     /locus_tag="PXB44_000557"
     CDS             complement(14915..16246)
                     /gene="rsmB"
                     /locus_tag="PXB44_000557"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL5281069.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(16298..16723)
                     /locus_tag="PXB44_000558"
     CDS             complement(16298..16723)
                     /locus_tag="PXB44_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5281070.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(16816..19464)
                     /locus_tag="PXB44_000559"
     CDS             complement(16816..19464)
                     /locus_tag="PXB44_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL5281071.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(19666..20628)
                     /locus_tag="PXB44_000560"
     CDS             complement(19666..20628)
                     /locus_tag="PXB44_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL5281072.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
BASE COUNT         6144 a         4470 c         4444 g         5649 t
ORIGIN      
        1 aaaatgtgat cgccttctaa aaatcaaatt tgcttgctcg acagtaacgc tacaagcggt
       61 taaatttgga gaaaaattta caaaaaggat agattatgca gctgcgacac aaacagataa
      121 tggaatatat acaacagttt gatgaagcta acgttcaaga attggcggaa cattgtgggg
      181 tttcgattga aactatccgc cgagatttaa ataagctaga taaagatgga ttattacatc
      241 gcactcacgg tggagctgtg agccataaaa agcgagatat tggtcgctca tttaccaccc
      301 gacaacggct gaacagtgag gcgaaacgca gtatcgcaga aaatgtgttg gaatatttac
      361 aaccagaatc ggtgattgct ttagatgcca gctcaacctc ttggaatgtg gcacagcgta
      421 ttccaaatat tccttgcacc gttgtcagca gttcaatgcg gattatctac agcctttcac
      481 acaaaccgca cattaaaacg attgccaccg gtggtattta ttccgaaaaa tacgatgcct
      541 tctatggtcc attatccgag cagttactga cccgtttaaa aattgatctt gccattcttt
      601 cttgtacagg cattgccgat ggggcgattt gggaatcaaa tgaaattaat atttccctta
      661 aacgtaaatt aattgcgaac agtaaacagg tttttttatt agccgatcac agtaaatttg
      721 agcgtaaaga cttgtttcgt atcgaagatt tatcttgcat tgataaatta tttatcaacc
      781 aacccccacc ggactttctc aaaaattatt gcttagcaca tcaaattgaa atcattattt
      841 agtgatttct gcccgatttt attcagaaat aaaacaaatc gggcattctt ttttcactca
      901 tttttaccaa aatcccccat tttttgtgat cgcactcaca aaatttaaac taaattcgca
      961 tttttcttca taactcatat tgcgtatgca taataaattc aatcgcaaat ttttttaacc
     1021 aatcgggaga actatttatg acaacgttga aatctacccc tgtaaaaatt ggtatccgcc
     1081 caaccattga cggtcgccgt ttaggagtgc gtgaatcact tgaagatcaa acaatgaata
     1141 tggcaaaagc cgttgcacaa ttaattcaat ctgaagtccg ccacccaaat ggtgatttcg
     1201 ttgaatgtgt gattgctgat tccaccatcg gtggtgttgc cgaagctgcg gcttgtgcag
     1261 aaaaattcaa acgtgaaaat gtcggagcgg tgattaccgt aaccccttgc tggtgctacg
     1321 gttctgaaac cattgatatg gacccacata tgccgaaagc catttggggc tttaacggaa
     1381 cagaacgccc aggtgcagtt tatttagctg ctgctttagc aggtcatagc caattaggtt
     1441 taccggcatt ctccatttat ggcacagaag tacaagaagc tgacgatcaa tctattccag
     1501 acgatgtgcg tgaaaaatta ctccactttg cccgtgccgg cttagcagtg gcaaccttac
     1561 gaggaaaatc atatttatct atcggctctg tttcaatggg tattgccggt tctatcgtaa
     1621 accaacaatt cttccaagaa tatttgggta tgcgtaacga atatgtggat atgaccgaaa
     1681 ttaaacgccg tttagatcgc catatttacg atgccgaaga atttaaatta gcaatgagct
     1741 gggtgaaaac ctattgcaaa gaaggtattg atgtcaacgc accggaaaat caacgcagcc
     1801 cggaagagcg tgaaaaactc tgggaagacg tggtgaaaat gaccattatt gcccgtgatt
     1861 taatggtggg taatcctcgc ttagcagagc taggctatag tgaagaagcc ttagggcata
     1921 atgcgattgc tgccggtttc caagggcaac gccaatggac agaccatttg ccaaacggcg
     1981 attttatgga agctaccttg aattcgacct atgactggaa cggcgttcgc gcaccataca
     2041 ttatggcaac tgaaaacgac agcttaaatg gcgtgaatat gttgttcggt aacttattaa
     2101 ccggtcaggc acaaatcttt gccgatgtgc ggacatactg gagcgaagac tcggtagaac
     2161 gtgtcaccgg ctggcgtccg gaaagcggtt ttatccactt aatcaactcc ggctctgcgg
     2221 cgttagacgg aacaggtcag cataccgata gagcaggcaa accaactatt aaaccggctt
     2281 gggaagtgac tgaagaagac ggtaaacgtt gtttagaaaa cacctgttgg tgtccggcag
     2341 ttcacgaata tttccgtggt ggtttatctt cccaattctt aaccaaaggt ggaatgccgt
     2401 ttacgatgca ccgtatcaac ctgatcaaag gtatcgggcc ggttttacaa attgccgaag
     2461 gttggtctat cgacttgccg gaaaatgtac acgacatttt aaacaaacgc gccaatgaaa
     2521 cctggccgac cacttggttc gtacctcgct taaccggaaa aggcgcattt accgatgtgt
     2581 atagcgtaat ggcgaactgg ggtgcaaacc actgtgtggc aaccttcgga cacgttgggg
     2641 cagacttaat taccctcgcc tcaatgctcc gcattccggt ttgtgtgcac aatgtggaag
     2701 ataaagacgt attccgccca agtgcgtgga acggtttcgg tcaagacaaa gaaggtcaag
     2761 actaccgtgc ttgtgctaac ttcggaccat tatataaata atacacaatc accctctccc
     2821 cttgtgggag agggacagcc aagcaattcg ttcagaattg aatggcaggg agagggctac
     2881 aagcggtcgc ttttctctca aaatttacaa atccctctct ctgattaaat tgctaaacgc
     2941 aatttaatct atctctctaa aagggagata ttaggataag taaaggagat aacaatgcca
     3001 atttctctta ttttcgactg tggtgccacc aacctccgta cgattgcgat taatcaagcg
     3061 ggtgaaattg tggcagccca tcatctaccg aataacacac aaccagatgt ggaaagccca
     3121 gaatttcata tttgggatat tgaagagatc tggtcgaaat taatgacctg tgccaaacag
     3181 acgctttctc aactttcaga acaacaacgg caagagattg ttgggatttc agtaaccacc
     3241 tttggcgtgg acggtgcatt atttgacaaa aacggcaagc agctttatcc gatcatttct
     3301 tggaaatgcc cgagaaccct accgattatg gaaaaccttg ccaattatct tgatgttgag
     3361 gcgttatatc aacgtaatgg cgttggacat tatagtttca acaccttatt caaattattg
     3421 tggttaaaag agaacaaacc agaaatttat caacaagctg agagtttctt attcatctca
     3481 tctattttga cttaccttct tacaggcgtg caaacaaccg accgcacaat ggcgggaacg
     3541 tcaatgatga caaacatcga aaacgattcg tgggataaaa cagttttaga tttattaggc
     3601 ttaaccgaaa accattttcc tccaatgaaa agtgcaggag aagttgtggg aacattaaaa
     3661 ggagaaattt cccaagagtt aggtttaagc ggtcaaattc cagtaatttc ttgcggacac
     3721 gatactcaat ttgccatttt tggctcagga gctggctata accaacctgt gttaagttca
     3781 ggcacttggg aaattttaat ggttcgtacc ccaaaagcaa aaccacaatg gcaattcgtt
     3841 caaaacggct taaccattga gtttgatagc caatctggtt atttcaaccc aggtgtgcag
     3901 tgggtagctt caggtgtgat ggagtggttt ggcaaacgtt tcttctctga tatagccaat
     3961 actgaacatt actaccgcac aatgattgaa gaagcggcag aagtgcctgc tggctcaaac
     4021 ggtgtgaaat tagtcggcaa ttttgatggc acaacagcta aaacaggttc aattatcggc
     4081 ttatctatgc acatcactcg tggcgaaatt taccgtgcag gcttggaata tatggctcat
     4141 cgtctaaaag aagggttaga tgtattacaa gaagtcggtc attttaatgc tgagagtttg
     4201 atttgtgtgg gaggtggttc taaaaacgct ttatggaatc aaattcgagc cgatgtctta
     4261 aatagaccga ttgatgtagt ggatttccct gaaagtaccg tattaggcgc ggcaatgttt
     4321 acttttacag gtgctggtgt atttgaaaat ccaaatgcgg ctcaacaggc aatgaagcca
     4381 aatgtgaaac gggttgagcc atcgtcaaat agtcaatttt ataaataaag tttctcccct
     4441 ctctctgatt tgaggttcat tcagaacatc aaatcagaga gagggtaaaa aaggagttca
     4501 aaatgttaaa aggtattcac ccggcaattt ctccagattt attaaaagtc ttagccgaaa
     4561 tggggcacgg cgatgaatta gtgctttctg atgcacactt ccctgctcac tctattcatc
     4621 aaaacgtcat tcgtgcagat ggcttaagtg ttgccacctt attagaaggc atttctgctc
     4681 tgtttgaatt tgaccaatac gttgaagccc cacttgcaat gatgcaagca gtaaaaggcg
     4741 acacgctcga tccaagcgtt gaagagcgtt acctcaatgc aattaaaaaa gtaaacggaa
     4801 cagccccgaa aatagagcgt gtagaacgct tcgctttcta tgatagagcc aaaaacgctt
     4861 atgccgttgt cattacaggc gagttagcca aatacggcaa cattattatc aaaaaaggcg
     4921 taacaccggt ttaattacaa gcggttaaaa aagcagaatt ttttacaaat ataaaattaa
     4981 aaggagttca acaatggaca gaaaaatact ggcgagacaa atcattgata cttgtcttga
     5041 aatgacccga attgggttaa atcaaggtac agcaggcaat gtgagcgtac gttataaaga
     5101 cggtatgctc attacgccaa caggtacacc ttatgagcaa atgaccgaag attctatcgt
     5161 ctttgttgat ggcaacggca aacacgaaga gggaaaactc ccttcaagtg agtggcagtt
     5221 ccatttagcg gtttatgagg ctcgccctga attaaatgcg gtagttcaca accacgctaa
     5281 aaactgtgcc gcagtgtcta ttttaggtga acctatccct gccattcact atatgatcgc
     5341 ctgcaccggc actaatcaca ttccttgtgt accttatgcc ccgttcggca ctcaccaatt
     5401 agccgactat gtacgtgaag gcattaaaca cagcaaagca attttactcg ctcaccacgg
     5461 cttaatcacc gcagataaaa ccctcgaaaa agccctcggc atcgcccacg aggtggaagt
     5521 gatcgcagaa tggtatttga aactgctcgc tacaggaaaa cctatcccaa cgctaaacaa
     5581 agaacaaatg gatatcgtac tcggaaaatt taagtcctat ggttcttggg tggaaagcga
     5641 acaatagcct gccataaata acatacaagc ggtgatattt cttaaaattt ttacaaacgg
     5701 agtgcagtat gcaaaatgaa gtaatactca cgatgaaaaa catcagtaaa tcctttgcgg
     5761 gggtcaatgc gttaaaaggt gttgagctta cgctgcgtaa gggcgaagtt cacgcattaa
     5821 tgggcgaaaa cggtgcaggt aaatccacct tgatgaaatg cttgctgggc gtatatcaag
     5881 ccgatgaagg tgaaatcata tataaaggtc agcctgttcg ctttgagtcg gtattagagg
     5941 cacaaaaagc gggcattagt atgattttcc aagaactgaa cctcattccc catttaacgg
     6001 tggcagagaa tattttcttc gcccgtgagc cactcaaaca cggtttagtg gataaagaca
     6061 aaatggtgga agaatcggct aaattgctga atttctttga gattgatgtt gatccaaacg
     6121 atgaagtgag aatgttgtcg gtagccaaac aacagatggt agaaattgcc aaagcccttt
     6181 catttgatgt ggaagtactg attatggacg agccgacctc cgctcttacc gaaaaagaga
     6241 tcgataaatt atttgaattg gtcgatagac tgcgtgccaa aggggtgagc attgtctata
     6301 tttctcaccg aatggaagaa ttaaaacgta tctgtaacca tattacgatt ttccgtgatg
     6361 gcacctatgt atctaaccat aaattcaatg aaatcacgat ggatgagatc attaccaaaa
     6421 tggttgggcg ttcattggat aaccatttcc caccaaaaac cgctgtggtg acaaacgaca
     6481 ttatcctctc gattatgaat gctgaacgta agggagtgtt tgaaccattg aactttgact
     6541 tacgcaaagg agaaattctg ggcattaccg gcttggttgg agcaaaacgt actgaactgg
     6601 ctcgagccat cttcggagca gacccacttg atagcggtga gatttttgtg cacgagaaca
     6661 aagtcagcat taaagatccg agtgatgcaa ttaaagccgg tatagcctat ttgtcggaag
     6721 accgtaagct caatggtgtt gcagtaagaa tgtcgattcg tgagaatatc acgatggcat
     6781 ctatggataa agttgccaat caaataggtg tgatttctta cgatgaagaa gaaaaagcct
     6841 caaaaacttt catcgacaaa atggaaatca aaaccccaac gattgagcag aaagttcaaa
     6901 acttaagtgg tggtaaccaa caaaaagtgg taattggtaa atggttattc cgtgaagcaa
     6961 aagtgatgat ttttgatgag ccgactcggg gaattgatgt cggagcaaaa tatgctatct
     7021 atcaactgct cgatgagctt gctgccaatg gcgtgggtgt tatcgtgatc tcttccgaat
     7081 tgcctgaagt cttgggagtt tccgaccgca tcattgtaat gcgtgaaggt agaatgacag
     7141 gtatgctcga gactaaaaaa accaaccaag aagaaattat gcattatgca actggtgtta
     7201 aaaatatgtt tgctcgtgaa tatggggtgg aaaaatgaat gaaaaacaaa aagaaatgtt
     7261 aagaaaaatg gctgcattgg ctggcttagt actattaatc atctttttca gcgtaaccaa
     7321 tgaatacttc tttacctcca ataacattat gacagtaggg ctacaaacct ctaccattgc
     7381 gttaattggt attggggcaa cctgtgtgat cttaaccggt ggaattgatt taagtaccgg
     7441 ttcagtagtt gcactctccg gtgtggcagc tgcaatgatt gtaaatgccg gtgtacccgt
     7501 accactagga atgattttag ggattttggt cggcggtatt tgtggcttaa tcaacggcat
     7561 tttagtcacc agaatgaaat tgcctccgtt tattgctacc ctcggtatga tgatggttgc
     7621 ccgtggttta gcattgtatg ttaccaatgc cgcaccagtt tccggaatgc ctgaatcctt
     7681 tgccaatctc ggtaacggag cattatttaa aattgtggaa gaaggggcaa acggcttgcc
     7741 gaaagtggta tttgcaggta ttccttaccc tgtgattatt atgatcttta ttaccgtact
     7801 attcactttc gctctctcta aactgaaagt cggacgctat ctttacgcaa ttggttctaa
     7861 cgaagaagcc gcacgcttat ccggcattaa aaccaatatt gtcaaaatct atgcctatgt
     7921 tgccagtggc ttactctctg gtttaactgg cgttatcctt gcctcccgcc tcgtcaccgc
     7981 ccagccaaac ggtggggtct cttacgagct ggatgcgatt gccagtgctg tggtgggcgg
     8041 cacatcgctt atgggcggtg tgggtactat ccccggcaca ctcatcgggt cattcattat
     8101 cggggtactc cgtaatggtt tgaatatgaa tggcgtttct tcatttgttc aaatgattgt
     8161 catcgggctt gtgattatcg tggcggtctc tctcgaccag ctccgccaat ccaaaaaagc
     8221 cggtaaaaaa taatccactc ttaaaggaga attaaaatga aaaaatcagc attatttact
     8281 gcgaccctct ttggtttatc gaccttattt gcaggcagtg caatggcaaa atctgatgaa
     8341 atcgctgtga ttgtgaaatc tgccaactct accttctggc aaaacgtgcg taaaggggct
     8401 gaaactgccg gcaaagatat tggaggacaa tacaaagtca ccttccaagg gccggaagcc
     8461 gaaacagcga ttgacgccca agtgaatatg gtggacaacg ccgtaaaccg tggcgtggca
     8521 ggcatcgtac tggctgcctc agacccggta gcattagtgc cggcggtgaa aaaagcttat
     8581 gagagtggca ttccggtggt gcttatcgat tccggcatca acagtgacgg caaatactac
     8641 caatctttcc ttgccaccga caaccgtgca gcaggtaaat tagcagcgga aaaactgctt
     8701 gccaaagtca aaggcggcaa agtggcagtg atgagcttta cccaaggggc agcatcagcg
     8761 attgagcgta ccggtggttt tattgacgaa atcaaagcaa aagccgatta caaactggtc
     8821 ggcccgtact actccgattc ggatatgacc aaagcgttaa accaaaccga agacgtatta
     8881 ggttctaacc ctgatcttgc agcgatcttc ggggcgaacg aaccgactgc ggtaggaatg
     8941 gcacgagcgg tgaaacaaaa aggctttgcc ggcaaaattg tagcggtggg ctttgatgga
     9001 aactccgacc tacaaaactt cgtgcgtgac ggaacactag acggcatcgt ggtgcaatct
     9061 tcctaccaaa tgggctataa aggcgtggac accatcggca agctgattaa aggcgagaaa
     9121 gtcgaaaaag tgattgatac cggggtggtt tatgtcacga aagaaaacat tgatagcgaa
     9181 gaagcgaaag gtgttcttta ctaacattca agttaaacgg ggtggggttg cccacccttt
     9241 cagccgaaag gaagccaata tgtcaaatac ccttaccgat aaagccttag tggtgattgc
     9301 cgcacttggc ggtcgagaaa atattgtcga tgtttacgcc tgtattaccc gcctacgtgt
     9361 ggaggtgaaa aataccgaca ttgtggaatg ggataaaatt aaatctgtcg gtgcgttagg
     9421 cgttgtgacc tcaggctcag ttattcaatc tatttttggt agcgatgccg aagcctaccg
     9481 tgatgaaatc aaacgtattt tgaatattta gcctaagcac actcacaaca aaagaatcgc
     9541 aacaagcggt gaaattttta tcaaaatttg caaaaaaatg acgtaatttg accgcttgca
     9601 agttaatcga attgtttaac ttttaagaag gaaaatagta tggcaaatcg tcttatcctc
     9661 aatgaaacca gttaccacgg caaaggagca atccaaaata ttgttcacga agtgaccagc
     9721 cggagtttca aaaaagccct tgtggtaaca gacaaagacc tgatcaaatt taacgttgcc
     9781 caaaaagtca ccgccttgct tgatgccgca ggcttgcctt atgagatttt cgatgaggtc
     9841 aaagccaacc cggcagtcga tgtgatcaaa gcgggggtcg caaaattcaa agcctccagt
     9901 gccgattatt taattgcgat tggtggcggc tcaccgattg atagtgccaa agcgattggt
     9961 attatcatca ataaccctga gtttagcgat gtactttcat tggaaggtgt cgccccaacc
    10021 caccaaaaat gtgtgccgat tatcgccatt ccaactaccg caggtacagc cgcagaagtc
    10081 acgattaact atgtcattac cgatgaagcg aaaaaacgca aatttgtgtg cgtggacgtt
    10141 cacgatgtgc cggcagtggc gttagtcgat ccagatatga tggaatctat gccgaaaagc
    10201 ttggctgccg caaccggtat ggacgctctc acccacgcca ttgaaggcta cattaccaaa
    10261 gcggcgtggg aattaaccga tgctctacac cttaaagcga ttgagctgat cggccgttca
    10321 ttgcgtggtg cagtggagaa aaatccgaaa gatgtcgagg caatggcatt gggacaatat
    10381 gtggctggaa tgggcttctc caacgttggg cttggcgcgg ttcacagtat ggcacacccg
    10441 ctttctgcct attacgacac cccacacggc attgccaacg cagtattact gccgtatgtg
    10501 atggaattta ataaaaatta caccggcgaa aaataccgtg aaattgcccg agcaatgggg
    10561 gtaaaaggcg tagatgaaat gagccaagaa gagtaccgca acgccgccat tgctgccgta
    10621 caacagcttt cacaagatgt cggcattccg ccaaaactgc atcaaatcgg cgttaaagaa
    10681 gccgatttag cggcattatc ggtcgatgcc tttaatgatg tctgcaccgg tggcaacccg
    10741 agagattgta cggctgagga gctgttggag gtatatcgga tcgcatacta agttctatct
    10801 ttgttaaata gacaagcggt gattttttaa taaaaatttg caatttctgt taaaaaaata
    10861 ctgatagtca taatgcccga tttctcatat ttatggaaaa atcgggcatt ttcgttgctt
    10921 ttttatcgcc aagaaatgtg attttgttca catttatatc tataaattaa caaaaataaa
    10981 acaatttgtt tgcaaaggcg gttcagttac cgaatgatag tcatcaactt ccactgtgga
    11041 taaaaaagga gcaactcatg gtgaaacaac ataaaatgta tattaatggt gaatttgtgc
    11101 ctgcgttaga tggtcgcact ttagcagtct ataaccctgc caccgaaaaa gctatctccg
    11161 aagtccctcg tggcggagct gatgatgcca aaatcgctat cgatgctgct gaagccgccc
    11221 aagaagcttg ggaacgttta ccagcgattg aacgaggaaa atatttacgt caaattgctg
    11281 tggggattcg tgagcgtgcc gatgaaattg ctcgcactat ttccgaagaa atgggtaaaa
    11341 ctctgtcctt agcaaccgtt gaggtacatt ttaccgctga ctacttagac tatatggcag
    11401 aatgggcgag acgttatgaa ggggaaatta tccaaagtga tcgcccaaat gaacatattt
    11461 tcctatacaa aaaagcgatt ggcgtcacca ccggcatttt accttggaac ttcccgttct
    11521 tcctcattgc ccgtaaagcg gctccggcat tagtaacagg taatactatt gtgattaaac
    11581 cgagtgaaga tgcacctaat aatgcgattc tcttttcgga aatcgtgcat caagtcggtt
    11641 tacctaaggg ggtatttaat gtcgtcaccg gctatggcaa tgaagtcggg ccggaacttt
    11701 caggcaatga aaaaatcggt atggtttcct ttaccggtag ccagtttgcc ggacaaaaag
    11761 tgatggaagc cgccagcaag aatatcatta aagtcaattt ggaattgggc ggaaaagcac
    11821 cggctatcgt attagacgat gcagatcttg agcttgctgc taccgctgta gtaaattctc
    11881 gaatcatcaa ttcaggacag gtttgtaact gtgcagaacg catttatgta cagagttcaa
    11941 ttaaagagca atttgtccaa aaattggtag agaaaatgca agcggtgaaa tatggtaatc
    12001 cattagaaca acaagacatt gatatggggc cgatggtaaa tgaaaaaggc ttgcataaag
    12061 tgcaggaaat ggtgacacac gcactgcatc aaggaggtaa tctggtatta ggtggtaacg
    12121 tggttgaagg aacaggctac tactttgagc caactattat cgacaatgta gataactcta
    12181 tggatattat gcgtcacgaa atttttggtc cggtcattcc ggttgcgaca tttgatacca
    12241 ttgatgaagc gattcgctta gccaatgatt gtgaatatgg cttaacctct tcggtttaca
    12301 cgcaaaatat caataaagcg atgaaagtaa tttcccgctt aaaattcgga gaaacttatg
    12361 taaaccgtga aaactttgaa gcaatgcaag gctaccacgc cggttggcgt aaatcgggta
    12421 ttggcggtgc tgacggtaag cacggcttag aggaatacct acaaactcag gtggtttatc
    12481 tgcaatttga tgataaagta tagattcttc taaaaactcc cacttatcaa aataagcggg
    12541 agttttcttt taggttaacc ttgtatcaaa attgcaaaca aaaatattct aatgttaagt
    12601 aaatgtttaa taattattta tggttaactc tttttggcta attaatttgc aatttaaatt
    12661 aattgagtgc agaatgccat tagccttttt tgaaatcaca ttccataaaa tggcttgatt
    12721 aacacaacac aattatcact caaattagga ttttcattat gagtattttc caacaaacca
    12781 atccaaaccg ccgtcgttat gggctagctg tatttatcgg gattattgcc ggtattattt
    12841 cagcattcgt aaaatggggg gcagaacatc ctttcccacc tcgtagccct atcgatctgt
    12901 ttaccgctgc ttgcccgcaa cctgtgttag atgcaatgaa tgccgcagca gaccaagtgg
    12961 ctgccaaaac catcgcatta caagaatgtt cacgtgcatt tttaaaccca ccacacgttt
    13021 tcttacgtga ttatattggt atcgatccaa ctgaagcagc atttaccttt gctgaccacg
    13081 cgttcaactg gattggtgtc acccatatga ttttctcgtt agtctttgcg attggttact
    13141 gcattgtggc ggaaatcttc ccaaaaatta aattctggca aggtatcggt gccggtatca
    13201 tcgctaatat ttgtgttcac tacatcacct tcccggcatt aggtttaaca ccaccggttg
    13261 ctgaatggcc gttatatgaa cacatttctg aattagttgg tcatattttc tggttctgga
    13321 ccattgaggt gattcgtcgc gatttacgta accgtattac gcgtgaacct gatgccgaag
    13381 tgccgttaga tcaaccgttc cgttaattta aaattagctc gatttataca aaagccgcaa
    13441 tcacgttgcg gctttcttat tttacaagcg gtcaaatttt catgaaaatt taccaaacaa
    13501 aaagcacatt gtttcatgta gcaatgcgga gcttggcttt ataagaaaaa tacaccaagt
    13561 tggaacagtt tttcaatttc atcgatctgt ttcttatcgt tcacaaaaat aatcacacgg
    13621 tcattttctg caatcatggt ggatttgtgg gcgataataa cttcttcgcc tcgcaccacc
    13681 gccccaacaa ttgccccttg cggtagtttt aattcacgga tttgtcgtcc aaccactttt
    13741 gaagtggttg catcgccgtg agcgataatt tctaccgctc ctgcaacccc aagtttgagt
    13801 gaagaaactt gtaaaatatc gccttttcta acgtaactag cgagagcaga aatagtagct
    13861 tgttgtgggg aaatggcaat atcaatagtg ccaccttgaa tcaggtgtaa ataggcaggg
    13921 cgttgcacta aaataatcac ttttttcgcc ccaagccgtt ttgccagcag ggcggacata
    13981 atattcgctt catcatcact ggtgacggca aggaataaat caatattctc aatatgctcc
    14041 tcaaacagca actcttcatc ggaggcatca ccggtaagca ccatcgtttt agatagtttt
    14101 tctgctaatt tttcggctcg aaccggattg cgttcgataa tttttacccg atactgatct
    14161 tctaaatccc gagctaacga agagccgatg ctccctccac cgacaatcat aatccgctta
    14221 tgtgggcgat caagccgttg tagttcactc attactgctc gaatatgttg ggttgcacag
    14281 atgaaaaaga cttcatcacc tgcctcaata atagttgaac cttgtggcac aatcgctttg
    14341 tcttggcgga aaattgccac gacccgagca tcaatatgcg gtaaatgatc acgcagggct
    14401 gaaatcgggt aaccgaccaa aggcccaccg taataggctt ttacattcac gatactgact
    14461 aactcatcag caaaatgagt aatttgcaac gcccccggat agtgaataag gcgtaaaata
    14521 tcttctttga ctaaaatttc aggggcaata atatggtcaa taggcaatac atcatcatta
    14581 aacaaccgct ctcgttctct cacataatca gcactgcgaa tacgggcaat tttggtcggc
    14641 acattaaata aagtgtaagc aatttggcaa gcgatcatat tcacttcatc actattagtt
    14701 acagcaacta atagatcagc atcacctgca ccggcatcac gcaaggtttg aggtgaagca
    14761 tattcgcccc gaatcacctg taaatcgtgt ttatctttga gcttgtctaa acgagttggg
    14821 tcattatcca ccaagacaat atcattgtct tcactcacca aattttctgc cagcgtagaa
    14881 ccaacttgtc ctgcccctaa aataattact ttcattactc tgctactttt tgtagttttg
    14941 cgtagaagaa accatcacca ccgtttggtt gtgggaagaa ctgtttttct aacacttttt
    15001 cgccgttaaa atcgatttca agttgttttg cattcggtgt atcttgtaaa aaccgttgaa
    15061 tttgctcgct attttcttgc ggtaacaccg agcaagtggc atataacaaa atgccgtttg
    15121 gctttaacct ttgccataac gccttgagaa tctcaccctg taatgtggct aattctgcaa
    15181 tatcaccctc tttacgcaac catttaatat cagggtggcg gcgaatgact ccggttgcgg
    15241 aacaaggggc atcaagtaaa atgcggtcaa acagcacacc ctcttcaagc cattcctcag
    15301 gtttggaagc atcgccacaa attacctgtg caatctgccc cagtcggact aagttttcac
    15361 gcactcggct taaacggctc tcttcaatat ccaacgccat aacctttgct ttcggtgctt
    15421 tttctaaaat atgggtagtt ttgccacccg gtgcggcaca ggcatctaaa acggtttcac
    15481 cattttctgc cccgagcaac tccgctgacc attgtgcgtg agcatcttgc acggttgccc
    15541 aaccttgctc gaaatgtggc agttggttca catttaccgc tctctccagc aataaagcac
    15601 actttggcac gcaagcggtc ggattttctg aattttttgc aaattcaggc tcaaccagct
    15661 cgctaatcaa cgccgcataa tcattggtct tgatatgttg ttgattgact cgaatccaca
    15721 ttggtgggcg ttggttgttt gcttcaacaa tttctcgcca gttcggatag gcttttttaa
    15781 gtttattgac caaccattca gggtggagcg tttgccaatg tttatctacc ttcgctaaga
    15841 tttcttcttg ctctcgcaga aaacggcgta gcacaccgtt agtcaaggct cggaagctat
    15901 ccagttttag cgttttagtt gcattcacta cctcatcaac cgcagcgtgt gccggcactc
    15961 gcatataaag cagttggtat aaacccacta acagtaagca atgcaccaaa cgggttttgc
    16021 ctttcagcgg tttttcaact aataatttga tgatcgattc caagcgaggc agcacacggc
    16081 acactccgaa ggtgatttct tgcactaagg gtaaatcttt tggttcaagc tgtttttgag
    16141 cgtcaggaat caaggtggaa agtgatttac cttgatctaa cacctgtaaa ataatggttg
    16201 cacttattgc tctagaagaa agtgctttga acgattgttt tttcataaat aaaaatgcca
    16261 ttccgacaga atggcactat tgtaactgaa tgcgggatta tcttaaaact aaaattggct
    16321 tggtggtgtt gcgagccact ttaaccgtat tagagcttaa gcccttcgca tttggtttgc
    16381 tgcttgccag catcataatt aaatccaccg acttttcatc ggcaatacgg caaatttctt
    16441 cataaatcgt accatacgcc acaatgtgct gcacttttga accttcagga aaatggtttt
    16501 tggtaaattc gtgcaatttt tggtttgctt cagccaccac ttctttatcg aaatttttcg
    16561 gcaagaatgc agaaattaag ctgttatcca ccggctcaat aatagacaca acccgataaa
    16621 ccgcattcgg attttttgcc gtcatttgta atgccgtatc taccacatat ttagcacttt
    16681 ttaggtcggc taaatcaatc gcaataagta atttgttata catatttatt ccgtaggggc
    16741 aggtcctgtg cctgcccgaa tattaactaa aatagcggac gggcacagga cccgtcccta
    16801 catagcatat aaatactatg caataacaca ctgttttttc actctacggc gttggtttgc
    16861 cacaatacca aataccaaca atagagcagg aatgaagatc caatctttag aaggagcatc
    16921 ttgcggtacg gcaatgtagt gaatggtttg atcccagttt aagcctgctt tagctgccgg
    16981 agaatccatt tctaccatat caatcaccac ttttggtgtt tctacgccat caactttgat
    17041 tttttcatcg gtttgaatta gcgttaagcc taagtttttc aaacgctctt cgccggttgc
    17101 tccttccggt acaggtaatt gagcgtagaa ctcaatttct ttaccgtaag ggtttaatcc
    17161 ctctactttt aaagtaacat tttgacctac tttgcttgca agcaactgtt cttcaaactg
    17221 caccggctcg atatgacgtt cagacggata aattctgtcc ataaagaagc ccggacggaa
    17281 caaggcgaaa gcggctacaa ccagcaacgc cgtttcccac catttgtttt tagtcacaat
    17341 atattgcatt gtagcagagg taaaggcgag aaccccaaca gttgcggtaa taaacacaac
    17401 ccagccgtgc cccggataac gcagatcaat taataataaa tcggtattaa aaataaataa
    17461 gaacggtaaa atgacggttc ttaaactata tttaaatgcc accataccgg tattaatcgg
    17521 gctaccgcct gaaattgctg ccgcagcgaa cgaggctaaa ccaacaggtg gagtcacatc
    17581 tgccataatc ccaaaataga atacgaataa atgcaccgca atcaacggca cgattaaccc
    17641 gttttgacga ccgacttcca tgataaccgt tgccattaat gatgacacca caatgtagtt
    17701 tgcagttgtc ggtaagccca ttcccaaaat taagctgaat acggctacca agatcaacat
    17761 tagcacaatg ttgcccattg ataggagttc aatcacactt gaaagctgaa ccccaaagcc
    17821 cgttagtgcc acaacgccga caataatccc tgctgttgcg gtagcgaggg cgataccggt
    17881 catattacga ccgccagcct caaggctatc taccacatct cgaacacctt gtttgataat
    17941 ctctttggtt acgctctctt tacggaagaa attcagtaac gggcgttggg ttaattgaat
    18001 aaaaattaag gtaatggttc cccagaatgc ggataagccc ggcgaaagca tttccaccat
    18061 taagcaccaa agtagcacaa ccacaggaat aaggtaatgt aaacctgcat ttacggttgg
    18121 ttttcttttt ggtagagaaa caatttctga gtttggatcg tccgcttcta aatcagggta
    18181 gcttgccaca cggcgaatag ccagtagata ccataagcct aagaaagcac agacaatcat
    18241 aaacgcataa tcttctgcca catcacgaat ccaacctaag ccgtagtaaa cgccaatcgc
    18301 taatcctacg atcactaaaa ttgaagctac ggttcttagc aagaagacta aaatcgggct
    18361 tggctcttcg gctcttggta agccttgtaa attcatctta caagcttcta aatgcacgat
    18421 atacaccaac gcaatatacg aaattaacgc aggtaagaag gcgtgagtaa tcagctggct
    18481 atacggcata ttcacatatt cgatcatcaa gaacgctgca gcccccatta ccggtggcat
    18541 aatttgaccg tttactgatg aggcaacttc caccgcaccg gctttttctt tggtaaagcc
    18601 gacccgtttc atcattggaa tagtgaatgt acctgtcgtc acgacgtttg caatcgaaga
    18661 gcctgaaatc aagccggtta aagcggaaga aaccaccgcc gcttttgccg gaccaccacg
    18721 caaatgccct aaataggcaa aggcggtttt aataaagtag ttacctgctc ctgctttatc
    18781 gagcaacgcc ccaaataata cgaacaggaa cacatatttg gtcgaaaccc caagggctac
    18841 accaaacacg ccttcagtgg taatccattg ttggttgata atttgagaaa gcgaacctga
    18901 gcggtggctt acaatccaat ctgccgggaa atattgcccg aagaagttat agatcaggaa
    18961 tacaatcgca ataatcacaa gcggtaagcc aagactacgg cgggtcgctt ccagcaccaa
    19021 taaaatccct aaacagccgg cgataatatc ttgcgtattt ggcgctccaa aacgtttcac
    19081 caaaccttca tagaagaaaa tataatacaa cgctaaccca gctcctacga tggcaaacac
    19141 ccaatctatt ttaggaatat gatgtttagg cgagctggca aaagcagggt aagataggaa
    19201 cgccaagaac attgcaaagg ctaagtggat agaacgggct ttggtgtcgt ccaccaccac
    19261 attcaaatta atgcctgcgt gagtaagcca ttcttggaat acaaacggca atggagaagc
    19321 ataataaatt tggaacacag accaaagaat ggcgacatac acaatggctt ttttcgcaag
    19381 accggacggg gttcgccccc cactgtcatt ggaggcaacc atatcttgta ggtcatcgta
    19441 atcaacctta gattcaactg acataaagac tcctcaaaac gcaagcggtc attttcttat
    19501 taaattttac aaatttcctc aaaaaacaac cgcttgctcg tgaaaacact gttgataagt
    19561 tatcaattaa aggtgaaaat cgataactta tcaacagttt caaaaaagca aaaaaccagc
    19621 acgctaaaaa acgtgctgat tgaatcaaat tacagaaagg aaatattact taatccaacc
    19681 tttctcttta tagtaacgaa ttgcaccttc gtgtaacgga gcagaaaggg cgtttttgat
    19741 catctcttct tgttttaagt gttcaaacgc cggatgtaaa cgtttgaaac ggtcgaagtt
    19801 atcaaatacc gctttggtta cattgtacac tttatctgca tccacatcgg cagaagtcac
    19861 taacgttgca tatacaccga aggtttctgt cggctcatcc gaacctttat ataaaccgcc
    19921 cggaatcgta gctttggcat aatatgagtg atctgccact aatttatcaa tttcagggcc
    19981 ggttaccggc actaatttcg catcgcaaga agcggctgct tcttttaacg caccattcgg
    20041 gtgacctacg ttataagtga tggcatctaa gttgttatca cacattacag atgccatttc
    20101 tgccggtttc aattcagaag ccactttgaa atctttatcc gtccagcctt ttgccgctaa
    20161 aatcacgttc attgttgcac gagtaccgga acccgggtcg cccacgttta cacgttttgc
    20221 ttttaaatca tcgaacgatt taatgcctga atcggtacga gccattactg taaacggttc
    20281 cgggtgaagc gagaaaatgg cacgaatttt atcgtttttc ttaccttcaa atgagcttga
    20341 gccgttatag gcgtggtatt gccagtctga ttgtgcaatc cccatatcca tttcttttgc
    20401 tgcaatcgca tttaagtttg caaccgatgc ccctgttgaa ggagcgttac attttaaacc
    20461 tgtttttgct gaatcacggt ttactaattg gcaaatagat tgacccacaa cataataaac
    20521 accggtttga ccgcctgttc cgatagtcac aaatgtttca gccgcttgaa cagatgctgt
    20581 tcccatcaca aggcttgcaa ggaaagaaag tttgactaat tttttcattt ttaactccta
    20641 aaattaagga tcagaaaaca aagatttatt gttttgagcc actgctcaca ctacaagaga
    20701 tacaaaa
//
LOCUS       DANLUA010000019        20180 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLUA010000019 DANLUA010000000
VERSION     DANLUA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20180
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..522
                     /locus_tag="PXB44_000561"
     CDS             <1..522
                     /locus_tag="PXB44_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL5281073.1"
                     /translation="LYPQDKTQICQKFQKEQISQADLSDKQFRFLQYQFACQFHSLFK
                     DKKPTKEKYIHFTHLADYLDLSNVPNQEIYTIHSLAELPSSSEREPKIVLAKNAINEN
                     LPQEFYGIVITDYLFDITGKKMYGTLYSSYDNAREERNLTFKKEVLAELEARYSAWRY
                     LPYSEHLMGAVDD"
     gene            515..808
                     /locus_tag="PXB44_000562"
     CDS             515..808
                     /locus_tag="PXB44_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL5281074.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            857..4147
                     /gene="recC"
                     /locus_tag="PXB44_000563"
     CDS             857..4147
                     /gene="recC"
                     /locus_tag="PXB44_000563"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL5281075.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4283..7228
                     /gene="ptrA"
                     /locus_tag="PXB44_000564"
     CDS             4283..7228
                     /gene="ptrA"
                     /locus_tag="PXB44_000564"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL5281076.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7277..8923)
                     /locus_tag="PXB44_000565"
     CDS             complement(7277..8923)
                     /locus_tag="PXB44_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5281077.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            8999..11089
                     /locus_tag="PXB44_000566"
     CDS             8999..11089
                     /locus_tag="PXB44_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5281078.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            11160..12848
                     /locus_tag="PXB44_000567"
     CDS             11160..12848
                     /locus_tag="PXB44_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5281079.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            12860..15676
                     /locus_tag="PXB44_000568"
     CDS             12860..15676
                     /locus_tag="PXB44_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL5281080.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            15799..15930
                     /locus_tag="PXB44_000569"
                     /pseudo
     CDS             15799..15930
                     /locus_tag="PXB44_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(15985..16746)
                     /locus_tag="PXB44_000570"
     CDS             complement(15985..16746)
                     /locus_tag="PXB44_000570"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL5281081.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(16758..17789)
                     /gene="murB"
                     /locus_tag="PXB44_000571"
     CDS             complement(16758..17789)
                     /gene="murB"
                     /locus_tag="PXB44_000571"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL5281082.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(17893..18567)
                     /gene="crp"
                     /locus_tag="PXB44_000572"
     CDS             complement(17893..18567)
                     /gene="crp"
                     /locus_tag="PXB44_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL5281083.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(18629..18847)
                     /locus_tag="PXB44_000573"
     CDS             complement(18629..18847)
                     /locus_tag="PXB44_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL5281084.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(18850..19455)
                     /gene="slmA"
                     /locus_tag="PXB44_000574"
     CDS             complement(18850..19455)
                     /gene="slmA"
                     /locus_tag="PXB44_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL5281085.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19467..19922)
                     /gene="dut"
                     /locus_tag="PXB44_000575"
     CDS             complement(19467..19922)
                     /gene="dut"
                     /locus_tag="PXB44_000575"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL5281086.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
     gene            complement(19956..>20180)
                     /locus_tag="PXB44_000576"
     CDS             complement(19956..>20180)
                     /locus_tag="PXB44_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5281087.1"
                     /translation="TEHLLDDIAQQKISPNYIPHIIHFTGGIKPLEGKFNSSYVENVA
                     SLFRFYHNTSWEILTELPLGTIQLKPFITE"
BASE COUNT         6046 a         4191 c         4393 g         5550 t
ORIGIN      
        1 ttatatccac aagataaaac gcaaatatgc caaaaatttc aaaaagagca gatcagccaa
       61 gcggatttat ccgataaaca attcaggttt ttacagtatc aatttgcctg ccaattccat
      121 agtttattta aagataaaaa accgactaaa gagaaatata ttcactttac ccatttggcg
      181 gattatttag atttatcaaa tgtgccaaac caagagattt atactattca ttctttagct
      241 gaattaccta gtagcagtga acgtgagcct aaaattgtgc ttgctaaaaa cgcaattaat
      301 gaaaacttac cgcaagagtt ttacggcatt gtgataaccg actatctgtt tgatattacc
      361 ggcaaaaaaa tgtacggcac gctttattcc tcttatgata atgcccgaga ggagcgaaat
      421 ttaacgttta aaaaagaggt actggcagag cttgaggcga ggtattccgc ttggcgatat
      481 ttgccatatt ccgaacattt gatgggagcg gttgatgatt aaatataaag cagaaacggc
      541 tttgtcgctt ttggttgcca tcacgctatt tgcgattgtg gtgttgagtt tcagccagtg
      601 gcaatcggca caaaaccaca ggctaaatca acatttccaa cagcaacaag cggttatgat
      661 tttagaaaat cagcttgccc tcaaattagc cgggttagat tgtgaatcaa gtattactca
      721 aaatcagatt cgctatcaga ttcaatgctc tggcaatcgc ttaaccattc gctttccgtt
      781 aggcaaaatt gagctgaata atgattgaat cgtccctcgc tttggaggac aataattcaa
      841 ttagcgaggc gttattatgt ttacacttta ccaatccaac caaatttcat cactggcgga
      901 gatgctggtg aaaatccagc aggtaaaccc acttgaagat ccgtttgagc cggaaaccat
      961 tttgatccaa agtcagggaa tggcacagtg gctacaaatg cagattgctg aattaaacgg
     1021 tgtgatgggg aattgcgatt ttctctaccc tactacattt ttatggcaac agtatcggct
     1081 gttgttcccg gagctgccga aagagaatat ttttgagcgt agttcgttgg tttggcggat
     1141 tatgcggtta ttgccgagtt gcttgacact atcggagttt gctccgctaa agcgttattt
     1201 aggtgatcaa cctaataaag aacagtatca gcttaagctc tatcaacttt ctgccaaaat
     1261 tgccgaattg ttcgaccaat atttggtgta tcgcccccat tggctggtta gctgggaaga
     1321 gggcaactta caagcgatcg aaaatgagat aaaaattgca actgccgcta agccgattga
     1381 ttttgactta attcgagccg atatgaaatg gcaaagccat ttatggcaac agattgtatc
     1441 tgatctgcaa tcggaatctg atgagcaggt ttttactacc tcccaccgtg cctatttgca
     1501 acaaaattac ttcgctaagt tagatgacct tactgaaacg gaaaaagcca aattgccaaa
     1561 acgcattttc atttttggga tttcgtcctt ctcgccatta cagttagcgg tgtttaagaa
     1621 actcagcgag cattgcgata ttcacctgtt cttttttaac ccaagcgaaa aatattgggg
     1681 cgacagtgtg gaagaaaaag tgtggcaaaa attggcgtta agccacaaga tttcgcaaga
     1741 cgatctggat aacttattgg cggagcaaag aaataaacta ctgaatcttt ggggcaaaca
     1801 agggcgggat ttcttagctc aattaatcga atttgagccg aataccatgg atgtctttgt
     1861 cgaaccagag gaaaacagca acctcaataa actcaaaaaa cagattttca gcttagaaaa
     1921 tgacgccact attaccgatt taaccgccga taattcactc caaattcatt cgtgccacag
     1981 cccaatgcgg gaagtggaag tgttgcataa ccagctcctg aatttgtttg agcaagatag
     2041 cactttgctg ccgaaagata ttattgtgat gtcgccggat attaataaat acgctcctta
     2101 cattgatgcg gtgttttctc gctacaatac gggtaatcgg gatcctcgct atattccttt
     2161 ttcattatcc gaccaagcat ttacgcaggt tgatccgatt atccacagtt tcctgcaact
     2221 gctggcaatg aaagaaagcc cgtttaatgc cgaagaactg tttgatttac tggaagtgaa
     2281 tgcgatccaa acccgttttg gctttagtac ggaagatatt cataccctgc gggtttgggt
     2341 gagaaatgcc ggcgtacgtt cgggtgtaga aatcgagcaa cctcactggc aaaactacaa
     2401 ttcgtgggaa aacgggctaa accgtttatt gcttggctca agcctgaaag aagagcaggg
     2461 gatttgggaa aacacccttg cctttgggga tagctatggc ttaaatgccg agctggtggg
     2521 gcgacttgcc tcttttattg aggctttatt agcgtgggta aactttctgc aaacgccaca
     2581 gccgttggca gcgtggcagc aggcaataaa aaatttgact gctgatctgt atcaagaaaa
     2641 tgccgaaaat gctgcatcaa ttttattgtt gaataatgtg aatgatcaga ttatcgagca
     2701 aatttccgca acccattttt cagacgatct gcaaattgag gttttagcct tattgtttga
     2761 gcagcagctc agccaacatt cacagcagct taatttcttg gtcggacggg tgaattttgc
     2821 caccttgctt ccgatgcggg cgattccgtt taaggtaatt tgcttgctgg gtatgaatga
     2881 ggcagatttc cctcgccaac aacaaatcaa tagctttgat ttaatgcagt acgccccgaa
     2941 aaagggagac cgtgccaaac gtgatgatga ccgctacttg ttcttagaag ccttactttc
     3001 cgctcagcaa atgttttaca tcagctatat cgggcagtcg caaaaagacg gcaaagagat
     3061 gctaccgtcg attttggttt cccaactgtt agatgcaatg aatgaagctt taacgcctga
     3121 atgcaagcaa caattaagcg atgaagtgga agttttggtc agaaaacaac ctttaagtgt
     3181 gtttagcaaa aagaatttta cccacccacg agatcacgct tataacgcag agtggattat
     3241 caagccgact gttgccgtac aagattttct tagtgagcct ttaaccaatg aaagcgtggc
     3301 ttcgctagaa cttgatgaat taatctcttt tgtacaaaac ccgattaggt ttttctttct
     3361 aaaagtgttg gggatttcgt ttaacactta tgatgacacc attgatgaaa gcgaaatttt
     3421 tgagatgtcg gggctggata actaccggat tttaaacgaa ttattaacga taaatttagc
     3481 acaacagcaa cgtttttttg ccgaagagca actcaaaggc aatttacctg cttcttattt
     3541 tgctcaaatt gccacacaag atttgatcgg caaaattacc gaaatgcaag cggcgttaag
     3601 ccactattta gccagcgaaa gccgtatttt gagtgttaat cgggaggttg agctggaacg
     3661 ccaaaccctc accctaaacg gcaatattgc caacctatat cagaatgaag tggtgctatg
     3721 gcgagtaggg catttacgag ataaagacaa gattcagact tggatctacc atttattatt
     3781 gtgtgcctat ttccctgaaa aaacggtgaa attctattac cgagagggtg agcaagttgg
     3841 tgtgttgagc tttgaaaccg tctcgcagat agaggcatta tcgcaattaa gcatttattt
     3901 gaatgcttat ttggacggat tatcgcaagc ccagttagtg atctacgacc gaattgagga
     3961 atatctcaaa ggtgatggca caaacagcga acaagttctt atcgatttag cagagaaaga
     4021 tgagcgagac ggcagctatc tccatcggct tctcgctcaa actaaagagc tggattctca
     4081 agcgattcac caacgtactc aaagctggtt tagcttaatg aatcagaaaa tagcgaaagg
     4141 cgattaacta caagcggtcg tttttggctg aaaatttgca aaatgagaag gcgaataata
     4201 attcgcctac ctataaaaat agaatttttt ccgtatttta ctgtcaattt attactacaa
     4261 tgctaatttc aaaaggagat gtatgaaatt atcttttctt acccaattcg ttgcagcggc
     4321 ttttgccctc aatgtgatga cttaccctgc atttgcggaa gttgcaaaaa attctgaaaa
     4381 aacgaccgct agtcaacccc aatcccaact cggttttgag ctgattaaac agagcattaa
     4441 caaaagcccg aatgataaag cgatttatca aggtattaaa cttgataatg gaatggaagt
     4501 gctactgatt tccgacagtc aagcgaataa atcgttgatg tctgccatta ttccgattgg
     4561 ctcaatggat gacccgattt ctcagcaagg tttagcccac tatcttgagc atatgatctt
     4621 gatgggctcg aaaaactatc cggaaaccaa cagcttagac ggttttttaa ccaaaaatgg
     4681 cggtatgaat aatgcctcga ctgcttctca ccgcacggcg tattatctac aagtcaataa
     4741 cgaagccttt agcgaagcgg taacacgact ggcggatacg ttagctttcc cgttattgct
     4801 ggaaaccaac gccaagaaag aggttaatgc ggtcaatgcg gagatggtgc gagctaaatc
     4861 gcacgatggg catttattgc acagtgttaa tttagccacc gcaaacccgg ctcatcctgc
     4921 taccaaattt gcggtaggca ataaagagac gttatctgac aagccggata gcaaattaca
     4981 aaccgagttg gagcaatttt ataaacagca ttattctgcg aatctgttta aagtggtgct
     5041 ttattccaat caatcgattg agcagatggc aaaattggca gccaacactt taggcaaaat
     5101 ggaaaacaaa catttcagca taccgcaaac cgctgagcca cgttatcgtg atgaggacaa
     5161 atcggtattt attcaatata agccggtgaa atccaacaaa atgttagtca ttggctttga
     5221 ttttccgaat gacgaaatgc agtttaagca caaaaccggt aaatatattg cttatatgtt
     5281 aaacaataat actgacggca cgctttcgga ttacttgatt aaacaggggt tgtctgacgg
     5341 cggtattgag gcggttagca gtgccaatat cgggcgggat cgaggctcgt ttgacatcta
     5401 tattgaactg acagacaaag gtttgagcga gcaagatcaa attatttcat tggttttcca
     5461 acaaattgag aagatcaaac aagaaggcat tcaagagagc tattttagcg aagtgaaaga
     5521 gagcctgaag caggatttcc aacatttgca agtggaaaaa aatatggatt tcgtggaatt
     5581 tattgccgat caaatgctcc atttcccgtt agaacacgtt atcgatcagc cttatctgac
     5641 cgaaacaatg gataacgagg caattaaagc aaaattggcg gcaatgaata ttgataatgc
     5701 ccgcattatg ttagtcagcg atactgccaa aaccgacaag aaaacccctt attttgaagc
     5761 aggttattcg gtggcaaaaa tcacgcaaga acaaaagcaa aaatggctgg atttcagtaa
     5821 aaatccacag ctcaatttgc ctgaattgaa cccgtatttc actaccgatt ttaccttaaa
     5881 caaggtcgaa accggcttaa ctaaaccgaa attgattaac gaccaagcgg ggaatttaat
     5941 ctatacaatg ccgagcgtgt attttaccaa tgaaccgaag gctaaaatcg gcgttggctt
     6001 taatattcgc ccgagaaaag atgacttaaa gagcctgacc gcagcgtcta ttttgggtta
     6061 tatgaacaat ttagcccaaa ccaagttaga ttttcagtct tcggtggcgg gtatgaattt
     6121 aagtttagct ccggcggcaa acggtttagc cttaagtgca gagggttata cccaaaatct
     6181 ggctaaatta atgctagaca gcgtaaaaca gttcagccgc tttgagttta acgaagacgt
     6241 gttggcacag gctaaacaac gtttgcacca agagctggat cgtaacgaaa aagaaaacag
     6301 ccttaatcaa gctaatgcag cattaagccg ttttgcctct tacccttatt ttgatgtgcc
     6361 gaaacagcgt gagatgattg atagcattac ccttgcggat attgagcaac tacgccagcg
     6421 aattttgcag cagagcagcg gctttaagtt gttatcggtg gggaatttgt cggctgagca
     6481 ggtttctgct ctggcagagg atttacaagc ggtcataaaa aaccaaaaat ctgcaaatgc
     6541 taccggacag tatgtggatg tgagtaccag tcagcgtaag attaatgtga ttaaaagcgt
     6601 gccgaatgaa gataatgcgt tgctaatggc atttatgccg aacggctata acgagctgga
     6661 aagccttact cgagccaaat tgctcagaag cattatttcc cgttggtatt ttgatgattt
     6721 gcgtactgat aagcaactag gctatgtggt ttatgcgaca gataatatca tcgggaaaac
     6781 cgccggtatc cagtttatgg tacaaagccc gaataccaca ccggcaggga ttttagagca
     6841 taacgaacgt ttctttacac aaagtttcga acgtttaaaa aatctatctg atgaggaatt
     6901 tggaaaatat cgtgatagcc tagtggaaaa actgcaatat aaaccggagt ctttagcgga
     6961 agagttttct gagttcggtt cagactttag cagaaataac ccgaaatttg accgcttgca
     7021 acaggtgatc gaattaacca aagcactcac caaagcagat attgtggcat tttaccaaaa
     7081 agctgtgatt gaaaagcaag gcttcgtgtt tgtcagccaa gctgtcggca ccaaaaccaa
     7141 agctgaagac gttgcgaaat ttgacggttt tgaacagatc gaaagcattg aaaagctaca
     7201 acgagagttt gagattaagg attattagag agttattaga gagagttagg ggcgaaaaat
     7261 tttcgcccct cattttttaa tccacaattt tcaccggata taagtaagta tatccgaacg
     7321 gactttgttt gtagccttca acacgtttgc tggttggggc aaagttaatt gagtgagcca
     7381 cgttaatcca cggagcttgc tctcttaaga taacctgtgc ctgctcgtat aacttagtac
     7441 gttctgcttt atcagatgag cctaatgctt tttctaataa cgcatctaac tcagggttag
     7501 taaagcgagc atagttactg ttgccgatat ttggcgtgcc gaatagtggc gataagaagt
     7561 tatccggatc gccgttatcg cccgaccaac cgtaagttcc ggcggttaat tcaccggctt
     7621 tggtacgctt gatgtaatca ccccattcat aggtgactaa ttttgctttt acgccgattt
     7681 tttcccagtc ggcttgaata atttcagaca tacggcgtgg gttcgggtta gaagcacgca
     7741 ccacaggctg cacccataat tcggtttcaa aaccgttcgg gaagcctgct tcagctaata
     7801 gttgcttcgc tttttccacg ttatattctg aatccgctaa gctgtcgtta tagccccaaa
     7861 tggttggcgg aagtgggttt ttcgccgcaa aaccgttacc ttggtaaact acatcaataa
     7921 tcgctttttt atccactgct aagtttaacg cttggcgaac tttcacgtta tcaaacggag
     7981 ctttttcggt attgaatgcc acataagcaa tgttcaaacc cggttgagaa agtaaattga
     8041 ttttcggatc atttttcatt ttctcaatgt cggttgcatt tgggaagtcc attaaatcac
     8101 attgaccgga ttgtaatttc gcataacggg ttgttgcatc cggcacgatc tcaaaaatca
     8161 aacggtcaat gtcaggctta cctttccaat actctttgtt tgctacataa cgactggctt
     8221 ggtctagcac ataaccgcta aacacaaacg gacctgtgcc gattggcgtg gtatctaccg
     8281 cttcaggtat gccggctttc atcattttat ccgcatattc cgccgaataa atggaggtga
     8341 aatccatacc aaggctggat aagaatgacg catcacgttt ggttaaggta atacgaacgg
     8401 tgctgtcgtc cactttttcc accgatttca ataatgtcgg gaatttcatt gcgttgaaat
     8461 acggataagt gccgttagag acggtgtgat acggatgatt tttatctaac tgacggttaa
     8521 atgaaaatac tacatcgtcc gcattcaatt cacggcttgg agtgaagtct ttgtttgaat
     8581 ggaatttcac gcctttgcgt aatttaaagg tgtaagttaa gccgtcttcg ctgatttccc
     8641 agctttctgc caatgccggt tcaatatcgg ttgtacccgg cacaaattcc accaagcggt
     8701 tgtaaatttg ttgcgagctg gcgttataag ataaaccgtc catcattaaa atcgggctaa
     8761 aaccggttgg cgaacggcta acgcagttta caaaggtttt ctcggtttgc ggttttgctg
     8821 ctgccgcagt aggtttattt tcgttgcttt tatcatcgca agccgctaga gctaataccg
     8881 ctaaaacgct tgcactaatt ttggttaacg ttggaaattt catattattc tccgcttaat
     8941 cttaggatta acttacgaag attacctttt ccacaatgag gagtaaagaa caaaaagcat
     9001 tatttttgca aatcaatcca tagaattaga taccgtcaac gtcattgcca cccgtgatcc
     9061 aagtaaattt gccgatacgc cacaaaaaca aactaaagat gcacttttgg taaaacaagc
     9121 gacgagtgtg gcggcggcat taaaagcgtt gccgaatgtg gatgtgcaag gaggggcccg
     9181 tgcgattgtg cagaagccgt ctattcgtgg gttgagcgat aatcgtattg tgcaggtgat
     9241 tgacggagtg cgtcagaatt ttgatttggc tcaccgtggt tcttattttg tgccgatgtc
     9301 attgattcaa gaaattgaag taatcaaagg tccaagcagc tcgttgtggg ggagcggtgc
     9361 cttaggtggg gtggtggcaa tgcgcacacc gaatgcgttg gatttattga aaaataatga
     9421 caaattcggg gcaaaaatcc gtcaaggtta tcaaacggcg aataatcttt ccgaaaccga
     9481 agcctcggtg tttgcagcaa atgatcggtt tgatgccctg ctgagtggtt tctacaataa
     9541 tgctgacaat ttgcgtagcg gtgaaggcaa ggaactgaat aacacgggct ataagcaagc
     9601 cggcggttta gtaaaattcg gctggcaaat taacgatact catcgtttgg aattatccca
     9661 tcgtcatagc caatttaaac aaactgcacc gggtaataac gaggtagaaa atcaatttac
     9721 cgataatgat gttagtgcac aaattagtgc ttggcatgcg gctaatcctg gaaatccaag
     9781 taatccagta tatcttgcga aaatgttagc cttttacggt caattacatt caagtcttgg
     9841 tagtgtgagt tatttaagtg atcaaaaaat taccgataaa agcacctcac ttaactacta
     9901 tttcaaccca agcaatcctt atttgagcac acaagtgaca ttatatagca atagtaccac
     9961 tgaaaacgaa catcgaaaaa taagtggtgt ggaagatcaa actaaactaa gcactcgtgg
    10021 tgtgaatatc cgtaatcatt ccgaattgtc ttggttctcc ttggtgtatg gcgtggatta
    10081 tatgttggat aaagtagcca ctcagcgtgg gagaaataat acggacgcac aataccgtgc
    10141 taatccttat gatgctaaat ctaaaactac aggtgcttat ttaattgcac atattccgct
    10201 gtttgatgaa aaagtggtct tttctccgag cgtgcgttac gaccgtttcg atacttcaag
    10261 tgaaacggtg aaatataaag acagccattg gtctcctgct gcaaaactta cttggaaagc
    10321 gacaaattgg ttggatttaa ccgcgaaata taacgaagct ttcagagccc cttctatgca
    10381 agaacgattt gtgagcgggt ctcattttgg taccacagtg agaggattac ctattaataa
    10441 tattttctta actaacccta atttacgccc ggaaacggca aaaaataagg aagtaacctt
    10501 caatgtacat tttgataatg tcatctcgca aggagataaa tttaactttg aagcgactta
    10561 tttccgtaat gatgtgaagg atttgattaa tcttcaagta ttcaaagcaa atcctacaag
    10621 cccaatggaa tttattccca ctcgttccca atatcaaaat attgcgaatg cacgtttgag
    10681 tggtattgaa ttacaaactc gttatcaaac tgaacgtttg gcagtttttg ctaactacgg
    10741 tagtacaaaa ggtaaagata aagacagcgg ggaagccttg tcgaacattg ctgcaagcaa
    10801 aatcggcttc ggggtcaatt atgccgtggt gcaagacaaa ttcacggtgg gggcaaacgt
    10861 aacccgttat caagcacaac atcgtgtgcc gaaaaaccac ggcgtgactt accaaggcta
    10921 tacgctgacc gatctacacg ccacttacgc cccattaaaa ggcgaatgga aaaacctgcg
    10981 tttagacgtg gcggttgaaa acctattcga taaaaaatac caaccggcat tcagcttaat
    11041 ggaaggcagc ggtcgtaatg tgaaactcag tgcggcttac agcttctaat gtctttaacg
    11101 gaagacggca caagcggtcg ttttccggtt attttttgca atattaaaag ggagttacta
    11161 tgaaatttta tccgtcgctg atcgcaagtg cggtcagttt tgccgttgtt ttgagtggac
    11221 aagcggcagt gaatgtgccg gatgccggct cgttacagcg tgaattagac caacgtcaag
    11281 ctcaacaaaa agtcaaaccg agtggcgaat ggtttaatca agcggcaaaa gcaccttata
    11341 ccgcaacgga cggtcaagaa ctgacatttc cattaactca ggtacaagtt tttgatcgaa
    11401 ccaaccaaga aatctctacc ccggaattag ccccgatttt ggcaaactat gtgggtaaag
    11461 cggtttcgct gaccgattta agcaacttgg caaatgagat caccgagttt tatcgtagcc
    11521 acaattatct ggtggcaaaa gccattttac cgccacagga aattgaacaa ggtaaggtga
    11581 aaatcctgtt gtttaaaggc aatgtcggtg aagtgcggtt acaaaatcag agtgctttaa
    11641 gcgataaatt tgttgcacgc cttgccaaca ccacagtgaa tggttcggaa tttatcctaa
    11701 aagacgaatt ggaaaaattt gccctcacga tgaatgatgt tcccggggtg aataccggct
    11761 tggagctttc tgccggtaag caagcaggcg aggcgaattt gctgattaag ctccaaaatg
    11821 ccgagcgttt ctcaggctat gtatcggcag ataaccaagg caataaaaat accggacgtt
    11881 accgtttagc cacagggata aaagccaata atctgatagg ttggggcgat gagttgaaag
    11941 tggatttgat gagttccaac aacgctaatt taaaaaatgc gcgtttggat tattcgagct
    12001 tgattgacgg ctacgctacc cgtttgggct taacggcgaa ttatctcgac tacgaattag
    12061 gcggtaactt taaagccctg caatcccaag ggcatactca caatcttggg gcttatgtgt
    12121 tgcacccgac aatccgcttg ccggattttc ggttaaacac caaagtcagc tttaatcatc
    12181 aacgtttaac cgacaaacaa caagcggtca atgtggcaca aaaacgccaa gtaaacagct
    12241 taacggtggg cattaacggg tcgtggaatt cgcttaaaga cggcacaagc tatttctctc
    12301 tgtctgcctt gttcggcaat ttagctaacc acaccaacga gcaagcgcat tatgtggcaa
    12361 cagattttca gcccaaatcc cgttttacgg tctatcaata cagcctgtct cacgaacaag
    12421 cgctgccaaa atcctttgcc ttcaaccttg gcgtgaacgg gcagtttgcg gataaaaccc
    12481 tcgacagctc acaaaaaatg ttactgggcg gtttatacgg cgtgcgtggt catcaagccg
    12541 gtgtcgcttc ggtggatgag gggcatttag tgcaaaccga actcaagcat tatttaccgc
    12601 tcttttccaa aagcctgctg accagctcgg tcttctatga ttatggttgg ggaaaatact
    12661 ataaacacag cggatttttg gcgcgtggtg taaacaatag cgtgaaatta cagagtatcg
    12721 gagcagggct ttcgctctct gaagcaggca gttatgccat taacgccagt gttgccaaac
    12781 cgctgggtaa aaaaatcgag aatgcagata aacaccaatt ctggttctcc gccatcaaaa
    12841 ccttctaagg agacaaacta tgtttaaacg taatgcaatt tctctctgca ttttgaccgc
    12901 ttgcagcggt ttagcctatg ccaatacagc agatttgccg caaggtgaaa gggtgcgtgt
    12961 cgggacggca acgattgagc gcacaaccga taaaatgacc gtgaatcaat ccagcaataa
    13021 agtacaaatt gattggaaca gctttgatat tggtaagaat aaagaagtgg aattcaaaca
    13081 gccgacagag atggcggtgg catataaccg tgttaccggc ggaaacgcca gtcaaattca
    13141 aggcaagctg accgcaaacg gcaaagtgta tttagccaac ccgaacggtg tactcatcac
    13201 caaagatgcg gaagtgaatg tgggcggttt gttagtgacc acaaaagatt tagaaaaaat
    13261 cagcgaaaac ggcacaaaag ataaattcag tcgcaaatta gcgaaagaag gcaaaattct
    13321 taaccaaggt aaaattacgg ccaaaggcga gcgggctttt gtggtattgg tgggcgacga
    13381 tgtggctaat gaaggggaaa ttaacgccaa aaattattac cacaaagaaa cgaaaaaagt
    13441 caccgaatgt tatgacgatt cttggagctt ggtaaaacaa tgctacgatt ttgataaaga
    13501 aatcgtgaaa agtaccagtg gagaagtgca tttatcctcc ggagaaaatg tcacctttac
    13561 tttaagcgac cgctatattg atgtggcgtt agacgataat acggtcaata gcattatcaa
    13621 aaatgacggg gcaatcatcg ccaatggtga cattaccctc actgccaaag ggcgaaatca
    13681 agccttagac agcttaatta tcaataacgg cgtattgcaa gccaataagg tgcaaaataa
    13741 aaacggtacg atcattttat ctgccggtga aacccaatta cacgaaaaaa gccaaattaa
    13801 aggcgagaaa gtgagctttg ccgaagcttc ttattacggt ccgacagaag acattaaagt
    13861 ggtttccaaa accggctcaa aagtgaccgc acctgtgatc gatttcaaag gtaaaaccgt
    13921 gaatattcaa ggggaatttg ggcgtgaaga cagcaccgat ctctataacg atgaactaca
    13981 aatcctcaaa accacggtga atattgaagt cccgaatacg gaagatattc gcattgcaga
    14041 taccgacaac ggaggtagcg gttcctttat acaagcgggt gctttagcct cattacttgc
    14101 aaataacgga cgggttaatg tacagggcaa aggttttgat gtctcaggca agttgaatgt
    14161 gaatgccttt aaacaaacgg attccgtgct gaaatttatc aatcaggcgg gcatcaatat
    14221 ccacaatgcc gatattcacg caaacgggcg tttattcttt gtgacaaact tgccgaatga
    14281 caccgatttc caatcgaata tccgcattac cgacagcaaa atcaacttag gcaacggctc
    14341 aatgggcttt ggtcgcagta gcaaacgttg ggaaatgcac gatgtggttc gacgtgccga
    14401 tacggaactg cgtaaaaaat tcaatctgga tatgaaaaac gtggaattaa accaagtgga
    14461 tgattttgtg gtagcgggcg gtttccaaca agttaatttg gataacgtga aagcccgtgg
    14521 caaaaccaac ttctatctgg attccggcaa ctcccgcatg tttgtcggtg agcagattta
    14581 tgaatatggc gtaaccgata ttgaaaaacg cacccaattg gcgaatttag accagcgtag
    14641 aaaacgttgg aattcggata actatgatga gaccgatctc aactgggaat acaaccaacg
    14701 ctttgacggc tttgcagcaa aacacaatca ccgcgcgccg attgccgata cggaaatcaa
    14761 tattgccaat agtgatatca gcttagataa cggttttgtg catttaatgg cagaaaaaat
    14821 caatctggac gacagcaaaa tcaatatcac cttcaaccaa gacaacagct tggatgaaag
    14881 tacccaaatc aaccgcttag gactaaacgg caaagtgtcg atgaaaaaca gccatattaa
    14941 cgtagtgggc gatgaaaaag acggtatctc accggctcgc cattatgcca cgatgttctt
    15001 agtgggggag ctgcttggtg aaaagagttc tattttcctc aaatcccacc aaggtgcagt
    15061 attgagaacc gacagcaatg ttacaattgc cggtaaaaac agcaaagacg acctcaaaat
    15121 taccgccatt accaccggta aaaaaatggg caatgaaatc atcggaccgg acggcttgcc
    15181 aacctcggaa gataacgatg ccaacgccag caacaccgcc tttgccatcg gctatgaacc
    15241 taacgctacg gctatcgtcg aaaatgcaag cattaccgca ttagcaccaa acggcggctt
    15301 ggcatacacc ggcacgtcta aaacccaaat tgacgcgaag gataatgcta cctttacctt
    15361 ctttgaaaaa cctcgagccg gctatgacaa attgcttggc gacatcaaag gtaaccccga
    15421 taaattaacc ccacgcgatt tcgcccgttt atacgacaac attaaaggca tcaaagccag
    15481 cagcctatcc gaacaacagc ttggcctagc agacaccgcg aagaaaacca gcgaacacac
    15541 cttagcaaac agcctagacg tagaaaattt ggtttcagtg acagtgtgtg atgagcagaa
    15601 taagtgtgag gagcgtatgt taggtaacaa aaataccaat gctaaagtta ttgtcggaga
    15661 tatcaaattt gagtagcgtt gaagaaagtg gtagaagctc agacttgatc tgataaagca
    15721 ctataaaaag tgagagtttc ccgtttagaa tatgagtgtc aaaattcaat ctaaacaaaa
    15781 aggaaactct catgttttat tacttacaat cccctcatta aacacaagac cggtttatta
    15841 aatttagcag aagaaccggg caatatctct caagcctgca atgtggtggg tatgagccta
    15901 gatacattct attgctatca acaagcggtt atttttggca actaatttgc aaaaaaacaa
    15961 taaaatccga ccgcttgtag agggttaatc cgcccttagt gaaatttcac cgatgccgaa
    16021 atagcggagg ttgtcgccct gttttagaat gagttcgccc tgctcgttaa tgccttgcga
    16081 aatgccgtgg atttcttcgg cttcggtaat gagcttaacc gctttatggc ggaaaaggtc
    16141 gaaactttgc cagcgttcgg cataatgagc gaagccgaca agcggataaa ttttgagatt
    16201 tttttgcaat tcataggcta aacggcaaac caattcgttg cggttgaaat gatattgcga
    16261 taaatctgcc cacgcttgcg tcacaatatt ttcatccact tttgccattc ctaggttcag
    16321 cccaatgccg ataaccacat ctaaaccatt gcgattggct tgggtttcca acaaaatgcc
    16381 ccccattttt ttgccttggt aataaatgtc attcggccat ttaatttgaa tatcttttac
    16441 attttgaaca gtaaggcttt ccgcaatgat caaggctacc actaaactta agggcggtaa
    16501 atctgccgcc tgctccatcg ggtaatgcca taaaatcgag aaatagagat tttcactctc
    16561 gggcgaatac caagttcgac ctcgtctgcc acgtccggca gtttgttttt cagcgaggca
    16621 aaccgtgccg ttttccaaac gttggtgatg agtgagcaaa tattcattgg ttgaatcaat
    16681 ttcatcgaac acaacggctt gcccgcaaat taaagcattt tggatttgag cttggttaag
    16741 tttcattatt tccccactta gcgagtgatt ttttcgctat cgacctcacc gtttttaccg
    16801 ataaaacgca cttcaggctg aatttctacc ccaaattttt ctttcactct ctggcgaacg
    16861 gttttggcta gagccaagac atcttgccct gttgcatttt ctttattaat cagcactaag
    16921 gcttgctggg tatgtaccgc cgctccgcca atttgtaagt ctttcagccc ggtttggtca
    16981 attaaccaac ctgcggctaa ttttaccgtt ccatcagctt gagggtagtg aggaatggct
    17041 gggaatttgg cttgtattgg tgcaaattgt tcgtttgaaa tcaccggatt tttaaaaaag
    17101 ctgccggcat tgccaaattc gtcagggttt ggcaatttgg aggagcgaac cgcacagact
    17161 tcatcaaaaa tctgtattgg cgtaacggtt tccggattga gttgggctaa tgatccgtaa
    17221 cttagcaccg gtttccacgc tttggctaac tttaagccta ccgagataat cgcaaattca
    17281 tcacggtatt ggtgtttaaa cacactttca cgatagccaa attcgcactc ggatttggat
    17341 agcttaaaga gttctccgga acgtaaattt aacacttcca cgaaatcaca tacctgttca
    17401 aattcaaccc cataagctcc tatattttga atgggagcag agctggcgac acccggaatt
    17461 aacgccagat tttcaatgcc ggcaatattg tgtgccaacg tccatttaac caattcgtgc
    17521 cagttttccc caccttgaac gtgcaaatag tggaaatgtt cgtcttcgtg gtgcgtaatc
    17581 cctttgagtt tatttactaa caccacaccg tcaaaatcct ctaaaaacaa aacgttagag
    17641 ccttgcccga gaatcaagat tggcagttga gcgttaaagg ctttttgcca ttcgctcaat
    17701 agctgttcga ctgtggtaaa ttcaataatt tgggtcgctt ttgcaggcaa atggaaagtg
    17761 tgaaaagggg ttaagctatg tttcatcatc aaattagtta gttaagaagt ggaaataaca
    17821 agcggtgata tttttacaaa attttgcaat taagattttg ggcaggcaca ggatctgccc
    17881 ctacattcag aattaccttg ccccatatac cacaatggtt ttgccgtgag cagaaattaa
    17941 gccttcgtct tccaacattt ttataatgcg tccaacggtt tcacgggaac aaccgaccat
    18001 ttgcccgatt tcttggcggg taattttgat ctgcatacct tccgggtgag tcattgcatc
    18061 cggcatttta gctaagttga gcaaggtttg agcaatgcga ccggcaacgt ctaaaaaggc
    18121 aagattaccg acttggcgtg aagtttgacg taaacggcga gccatttgtg ccgataaata
    18181 cattaaaata tcggggttta aatggactag ttgtttgaat tttttatagg aaatttccgc
    18241 aatttcacaa ggacctttag tgcggacgta agcggtacgt tgttggactt tttcttcaaa
    18301 taaacttaat tcaccgacaa actcacctgt gcctaagtta gttaagagca tctctttggt
    18361 ttcatcatct ttgatgtaaa ccgacactga gccattcacg atgtagaaaa gtgattctgc
    18421 atcttcaccg gcgtgaatga gtgtatattt cgccggataa cgatggatat gacaatgggt
    18481 taaaaaccac tccaccgcag ggtcgtgaat agagggcgga ggagttacat tttccagagg
    18541 agctgttgaa agtgtcatat cttgcatagt atttcctata actcttatac taggttaaat
    18601 ttttagaatt ttaacacgaa attaaaggtt agcctaaaaa agattttttg tctttaatat
    18661 caaggctttc gtgtaattcc gaccaaacaa tcaccacttt atctgcctta agttgtgtga
    18721 gtaatcgttc tcttttttct gccagagaaa gttccctttc cccgtagtct gtgccttctc
    18781 gtaaaatgaa ggaatctaaa atattatgta aggttgattc ttcgagttct tgccaaggta
    18841 taatcataat taatcaaata aaggcttaat aaatgcccac tgtttttcaa aatgttggtg
    18901 ttggttatag ctgaaattag aacgaaccaa gcgtaaaaat tgcccttcgc agaaattcac
    18961 taaataaccg gctaaggctc tttcgtcttc aaagctcttg cgttcacgca gtttactaag
    19021 ttgcaaaata ttggcaaatt gtaactccaa gttatcaaag aattttgcca ctcgagcttt
    19081 gagcaactcc tcttcaaaca tcaaggcgtg tccggtcagg atgcgggtaa cgcccgggtt
    19141 tttgcgagca aattctaaaa tcacatataa aattgctcgc accgaattgg cactgctgtc
    19201 ttggcgttta ttggcgtgaa tatagctggt gagggtctgc tcgattttct cgattaacgc
    19261 ttcaaacatc ttggttttac ttgggaaata gcgatacaac gccccctccg acacccccac
    19321 cgcagcggct aaacgctcgg tagtgatacg ctgcattccc tcttcagaat ttaacaagct
    19381 gattaacact tccaacactt gttggcggcg ttccaccact gattttttcg gcattttaat
    19441 tttaggttct gtcattgcca atcctattat tgcttacctg aatgcccaaa accgccttcg
    19501 ccacggtcgg tctgctcaaa ctcttgcaca atgttaaaac ttgcctgaac caccggcaca
    19561 aagactaact gagcaatacg gtcgcccacg ttcacggtaa aatcagcttg gctacggttc
    19621 caaagcgaca ctttcagctc gccttggtaa tcgctatcga tcaagccgac taagttgcct
    19681 agcacgatcc cgtttttatg ccctaaacct gatcttggta agatcaccgc tgccaaattc
    19741 ggatcggcga tataaattga aatccccgtt gggatcaaca ccgtttcacc cgcttttacc
    19801 gtcagcggct cgttgagcat cgcccgtaga tccaagcctg ccgagccttc ggtcgcatac
    19861 gcaggtaatg gaaattcatt gccgatacgg ctatctaaaa tttttaaatc aatttgtttc
    19921 attctttttc ctttttgtaa atttttaaaa ctatattatt cagttataaa aggttttaat
    19981 tgtatcgttc caagtggaag ttcagtcaat atttcccagc ttgtgttgtg gtaaaatcga
    20041 aataaacttg caacgttttc aacgtagctt gaattaaatt taccttctaa tggctttatt
    20101 cctccagtaa aatgaattat atgtggaata taatttggcg aaattttttg ctgggcaata
    20161 tcatctaata aatgttctgt
//
LOCUS       DANLUA010000020        19696 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLUA010000020 DANLUA010000000
VERSION     DANLUA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19696
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1081)
                     /locus_tag="PXB44_000577"
     CDS             complement(<1..1081)
                     /locus_tag="PXB44_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5281088.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQL"
     gene            complement(1146..1577)
                     /locus_tag="PXB44_000578"
     CDS             complement(1146..1577)
                     /locus_tag="PXB44_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281089.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1581..3683)
                     /locus_tag="PXB44_000579"
     CDS             complement(1581..3683)
                     /locus_tag="PXB44_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5281090.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
     gene            complement(3710..3988)
                     /locus_tag="PXB44_000580"
     CDS             complement(3710..3988)
                     /locus_tag="PXB44_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL5281091.1"
                     /translation="MQAKTFYYLPLLLLSAMASASPANKPSDVTVKVFTSESYPKVFP
                     HSGDWATHSDSFSNTENYSWALWRPYSCCKKQGQVFLGHVDWKSTHTH"
     gene            4958..5389
                     /locus_tag="PXB44_000581"
     CDS             4958..5389
                     /locus_tag="PXB44_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5281092.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEHKKGNPFYACRVAAL
                     VGESDSPEYRYFDMNVVGKETETLIKKCQKAVSEEKKVLVSFVMADLWFDTFTYSSDS
                     KYHKKGDTGVSLKGRLIRIKMIKIDGEIVYQEKPKADEQSE"
     gene            5495..6175
                     /locus_tag="PXB44_000582"
     CDS             5495..6175
                     /locus_tag="PXB44_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281093.1"
                     /translation="MNSLQQFVKTLSPTVLNGRHFDGIVGDVVFVQNGYGKWLDQGFT
                     IIGFDKSIEEKDWIYLDWDCYWFGKNPAQVIPEKSWAQRSWLARAYSISLERFNSLGL
                     EREYNIVSDWDNPIYGTDIQLIEHSKEFFRWTEADDRGIFLVHNEMAFHFFKKEIKAS
                     SEPEYYQPIETFWKDATVVLNIEDLDMLERVGLEQTNTPALAKLTTFVQAARNEISNG
                     NIVFYRAF"
     gene            6201..6608
                     /locus_tag="PXB44_000583"
     CDS             6201..6608
                     /locus_tag="PXB44_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5281094.1"
                     /translation="MNRQGIYINGKQVFDSKGKEINSVQIINSTVQTVVGINKGYISM
                     AAGNNVLIVDGQTIALEPSPTINIEVFGNVTNLNAGSENVRVTGDAKSVSTVSGDVTV
                     DGNVTSNVKTVSADIHCKSILGKINTVSGDIYR"
     gene            6688..7050
                     /locus_tag="PXB44_000584"
     CDS             6688..7050
                     /locus_tag="PXB44_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281095.1"
                     /translation="MEDSFFDFDDISCYLGQWEAILEEYSDIVSIEDFWLVAKEFETV
                     PHFGNLYQELVISRLIQRFCTELDIEQDSDLVEFDYYINAIDTHFYINRQRICDIDDW
                     NEMLDKIRKEMTPAKLAA"
     gene            7163..7423
                     /locus_tag="PXB44_000585"
     CDS             7163..7423
                     /locus_tag="PXB44_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281096.1"
                     /translation="MTFELGRVCTTRAVAECFNQNALVELLNRYCYGDWGNLCEEDKL
                     ANQEAMKYGGRIVARYDVDDESLYIITEADRSCTTILFSYEY"
     gene            7582..8211
                     /locus_tag="PXB44_000586"
     CDS             7582..8211
                     /locus_tag="PXB44_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281097.1"
                     /translation="MKTKISLMTVFMLGLSGCVHQQDTAQNSDEITPMPDSLLTPQMQ
                     QLPPQDRIVSKDIYQEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVSNPKN
                     RFTANVQQGLRSTLTNTGLDLCYPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDISRTICFKARESSFQNPHKPAVAVTTTTIKTEVIEE"
     gene            8211..8984
                     /locus_tag="PXB44_000587"
     CDS             8211..8984
                     /locus_tag="PXB44_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL5281098.1"
                     /translation="MREISMKFNLCRKAIPLAVLPLLLSFALPTLAQNMQTETVSSSQ
                     STLQKQSQHQQSQLSDSLKQAKEWGLTVEEWSRYTELMKGERGMWSPNLDPLTALGIE
                     ARTAEEREKYARMLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSVGR
                     VVYFTRFDNCDSCKSDLGKILSYANNQTPLDIYIVGQNISDNDIRKWAADNNIDPIKV
                     SKRLITLNHDKGYWLRYSLGKMPAAYQIKQDGEWTSLVY"
     gene            8963..9733
                     /locus_tag="PXB44_000588"
     CDS             8963..9733
                     /locus_tag="PXB44_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL5281099.1"
                     /translation="MDITRVLVIVAVFLSNISISQAKPFNGFGSEIDSYIRMAAQRYQ
                     VSEAMLRGFVKMEDGWYGNISPTGATGVGQFTMGTWNWLSETERGKQLGMKLITAKNR
                     GSYADPRRDKYTNTLATALLARWHIEQFQLRNIPITDENLYMAHNIGLDGLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMTVSKQGHIHTGVAAFVY
                     PTSQMGKSVKWVEPDNKMIWVEPKNAMVWVEPQNTLQR"
     gene            9736..10242
                     /locus_tag="PXB44_000589"
     CDS             9736..10242
                     /locus_tag="PXB44_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5281100.1"
                     /translation="MQNKVKVLLFSLYVSHFSWADLTVIADFGGESAVRFYESLQPEH
                     DENAPNYPNSISASVSEADILPIVSHKLSPGTIKEKPFDLTGMQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVQTLSELNQLRDIVPELRILPTPGDDLAERIGIAHYPVL
                     ITESGVSQ"
     gene            10242..12443
                     /gene="traD"
                     /locus_tag="PXB44_000590"
     CDS             10242..12443
                     /gene="traD"
                     /locus_tag="PXB44_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL5281101.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAAVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHRNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTHLTSKDSRWNLVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTADERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNDGLPGAFKEKSKPVKINLHCD
                     EFNELMGDEFIPLINKGGGAGIQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALMDGSKLWKIRMPLPAVDKDDMMHQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYKTQIDHQVDNFGIDEPYDIDEEENDE"
     gene            12436..12789
                     /locus_tag="PXB44_000591"
     CDS             12436..12789
                     /locus_tag="PXB44_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5281102.1"
                     /translation="MSSAKMREEKRTNLLDLPNKYRNFNGEFSASCGLDNAEELLIHS
                     QSYFIEWFEQGYSFHQFAEKFADQGLSLWSADEVSMRHSDKSKDIFAFYLAFDNNPSG
                     YILVQCQLDREDSLQ"
     gene            12786..13481
                     /locus_tag="PXB44_000592"
     CDS             12786..13481
                     /locus_tag="PXB44_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL5281103.1"
                     /translation="MSEETKARRKSGLINLIFGKINMSLAAIAVSLIFSIILEWIGIA
                     FWWPQEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATSIIENVYNWLFVYTGVKE
                     WLTSPGNGSMEQWVHHYGRAYIESVIYVVITFIIRLIIIVFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVLYLSIPFSIHPNIILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(13513..13896)
                     /locus_tag="PXB44_000593"
     CDS             complement(13513..13896)
                     /locus_tag="PXB44_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281104.1"
                     /translation="MRYQMRYLALFLVVFPYVAHSLPSAQKVGNICHAENAQYNYGGL
                     WELSLRGKLICNYWQFLGDDERKKIELQLDEYGEFPIWNTSEQISEQIIEDDEYPQYW
                     EVEDWEDSDWDTESENEDTEATTEE"
     gene            14083..14409
                     /locus_tag="PXB44_000594"
     CDS             14083..14409
                     /locus_tag="PXB44_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL5281105.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPALASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHSDIETVKKGIQQYINPSRALPRDPNVLRTLSDDY
                     SKLRGQ"
     gene            14409..14642
                     /locus_tag="PXB44_000595"
     CDS             14409..14642
                     /locus_tag="PXB44_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL5281106.1"
                     /translation="MSYSPANAFSVASGIDPFRLKIMIAIIFLSLLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLACVTYFFIY"
     gene            14657..15046
                     /locus_tag="PXB44_000596"
     CDS             14657..15046
                     /locus_tag="PXB44_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL5281107.1"
                     /translation="MNRIKSLTKHLHSRYLAVMLFFWGSLQNAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEIIWIVLKGLVTLGAVILAAVAILTVVKALIGIYNEATSENGKKGWG
                     HFILALIIGFIVIFFCLWLVKLAVGLF"
     gene            15069..15434
                     /locus_tag="PXB44_000597"
     CDS             15069..15434
                     /locus_tag="PXB44_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL5281108.1"
                     /translation="MSEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGIVAAVKRGKPEAYLNRLVEKKMDDLFN
                     GHKFIKREGFWSIRRYRRK"
     gene            15438..16082
                     /locus_tag="PXB44_000598"
     CDS             15438..16082
                     /locus_tag="PXB44_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL5281109.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITTLKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTIFSEDSWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDVDSAQNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            16082..16966
                     /locus_tag="PXB44_000599"
     CDS             16082..16966
                     /locus_tag="PXB44_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL5281110.1"
                     /translation="MKRLPILLVSLLIGFSGSSLADVLMKWERKPLPVALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVTTGEIFLFDLTAA
                     SKVANPEEAIRVVFDGTVEKQSFSSNRDDESSDLPIANSRPQLPVPAALTRYAAQSLY
                     APLRTVEPLDGVRRVAHRLPKKITKLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNT
                     QITLDPRELQGQFYAATFQHNWLGGYGENEDTTTLYLVTEGYANNALIPETRPVKLVS
                     KKVKRTTKVETKPAQSAK"
     gene            16978..18432
                     /locus_tag="PXB44_000600"
     CDS             16978..18432
                     /locus_tag="PXB44_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5281111.1"
                     /translation="MKANKALFVILGIVFILVGGIVYALFGRSESVPQPTQQEILDIS
                     IKDLTPEQVRTMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     NDAVNNETRKLQEQIADLKMQLFDMATLVNNKPTTQESTPQVEAQPNTVPNKGFGTAG
                     DKLPIGGATMSNVDTSGMYWVEPSDQISLDASGKPVSGNTVATKFGFPKDFNGGQSAG
                     NNEKKGLPFFPETDTVTSSQGTAGNARGKNQMTSVYTIPSNSTFLDSVAMTGLIGRIP
                     IDNRVSDAYPFKVLIGRENLIANGIELPDVDGAIVSGTATGDWTLSCVRGSVKSMTFV
                     FNDGRIVSTSNKGNGEEIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGSSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVSIHIEKEIEIDYNAQGRKVKYGSKSTNSQLD"
     gene            18445..18843
                     /locus_tag="PXB44_000601"
     CDS             18445..18843
                     /locus_tag="PXB44_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5281112.1"
                     /translation="MKHLFCIMISALLITGCTTSTEELLPTNGSTMRDIWDKGASNND
                     LHLYRSQQGRTLDPVDYIPNSEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            18843..>19696
                     /locus_tag="PXB44_000602"
     CDS             18843..>19696
                     /locus_tag="PXB44_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDL5281113.1"
                     /translation="MMSFLSEVFSSFKPTKAGVISSEPNENTGESDIIQNKMSEVNNE
                     VQQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDINPYVVQFYCQDSDDLQPYIEQLRNYIS
                     PIAQNSAFSEEWLRVQAKHFKDIGKPEGLFIDDRVTNTAWSGRIRSTKMVIYRYVDKS
                     NDSNENLIIEQLNNACSRINGALTTAGLQLQRLDGEQIRQWLLRWFNPNPSINFEGLS
                     NNDCYHLFG"
BASE COUNT         5911 a         3780 c         3995 g         6010 t
ORIGIN      
        1 tcagttgtat aattcgttct gctaatgatt gaaattcttg gtcaaaattt tctttacttt
       61 catccattgt attcaaagca tgtccaaatt gacctgagtt tttaatttgc tcttttgtta
      121 aggaaaatac gggaactcgt agctcttggc taatagcaat caacgaatta aagtcggcta
      181 tgtgagccaa atcaaatggc tctaagtcag aattcgttga tttaagagca gcataaatag
      241 ctgtatcatc aatagcacaa tcaatagttt ttaataccgg cacaaaattc gtaatgactt
      301 catttctaat tcgctcaatc catttttcaa atgatttggc cggttgccct tttcttggtc
      361 gataacgttg ctgaatagta cctaaaaatt gtggcttatt tttaattgca taagatgatt
      421 ggttaaaacc attaagttct ttaaatgctt ttaactcatt atgccatttg cgaatatttt
      481 tagcgagcga accaactgct tgccagcaga aaaaatcagg tgatgtaggt acaataaagt
      541 aatcactaga cattaggata acttcattta atccacccac atttggacta agatcataga
      601 gtatgtaatc aatattgttt tgttcagcta ctttcttaat taaagtcgga agattgccgg
      661 gtatatttct tgttgctgga acaccggcag caatttttaa tgatacacta atttgcgaat
      721 caagctcgga aacattaagg tgaccaggta acaagaacag atttggattc agggtttgag
      781 tgatttggcc aacatttcca ctaaggtagt tatcagggct ttgtccgttt atcagtgctt
      841 caacaatacc ttccatagtc aaatgatgtc tattattgta aaaaagcgct aaatcactat
      901 cttctttgca ttgatcgtag ccaagtacca gaccggttag gttacattgt gaatccaaat
      961 cgactaacaa tacttgatag ccttgatttg ctaaactcca accaacatta aatgcagttg
     1021 tagttttagc aatacctcct ttatgattaa aaatactgat agacttactc ataataccca
     1081 taattctatg aaataattaa cccaccgaac tcatttgaga atggtgggtt aatgctttgc
     1141 tttagctagg ctgcaaatcc agattttctc atcagtttgc gatcaaagtt gtggcgaata
     1201 ggcttatcgg aacgacattc aaatgatgta atatcatttc ctgttttaat atcctgaatc
     1261 tgaacacttg cttcaaaagg tgatttaggg tctgcttcta tatgacctac aatataagat
     1321 tttcctgcat ttcttaattc gagcgagatt gttgtgaatt gtgaaccacc tgcaatctcc
     1381 gatgccggat ttttgagagc ttcttttaca ggatttttga atgtggtatt accgtgagag
     1441 tacacatagt ttcccccctc tttggtaact tttaacgttt ggttatctgt agttgtgcaa
     1501 gaatacacta cattgttatt tgcaaaagca ttcattgaga aaaatgctga taaagctaca
     1561 attgccattt ttttcatagg ttagtttcct tttggtgcgt tgataatgta atcggcttcc
     1621 tggttacgtc ttcgagcatt aacaccatta ttatgccctt gtaaatttcg gattgagtta
     1681 gctaaagccg tcatatcacc tgattgtgcg gagacggcag ccgctttttg aacactttct
     1741 agtttagata atgacccata gttgtatgat accgacgtta atgctgcttg tgcttgagga
     1801 gtcaatttag cccaaacgtc ggcaccaaca ttaccccttg caacattcat aaattgaggc
     1861 attcttcgtg ctaaatcacg ttcagcatct tcccggctta cataagtgcc tttttgaacc
     1921 cgatgaacca taccatctgc agtagtatag gtgtcagagc catatccggc acggttagca
     1981 tttacatccc atttcccgtg gcttaaatat ccttcaaact gtttgataag agccgttgct
     2041 tgttcattcg cattgccgtt catttgggca atttgggtat agctccctac attcacgtta
     2101 ctgacaatgt ttgttgggga ggctttatat gctccataaa aatcactcat gcctgagccg
     2161 tctgatttgg ggatagcaat atatctatcg ccaacggcat tttgtgcaga attagcaatg
     2221 ccactggtgt tgagattatt tgctccggct tgattgctta aattggcaaa gcgacggttt
     2281 ccatcaccca tcgcattatc atcaatagca agagcttgtt cgttcatctg ttgagtaatt
     2341 gcagctaatg ctgtattacc gttgattgaa cgttgcagtt ccatttcgag tttaacttgc
     2401 tcaatttctc tgtcaagtaa tttgaggttt ctttctgctt cttctactaa ggaagatttt
     2461 ataacatttg gctcacgcat accggctaac attgctcggc gaatcagtag caatttttcc
     2521 atttctttag aaatagctaa ttcttgcgat aaccttgcac ccagcaaggc tgcatccggc
     2581 gaagcagcaa tggcttctaa tacgcctttt gttacactga ccgttgatgt actcagtgaa
     2641 ttgagttgtt ccggcgttaa actatctaag tgattcagtg ccgcttctag ctttgtggct
     2701 ttttcaatag ttgtttgttc aataaaatgg gcgattcctt tcccggcttt agctccactt
     2761 gaactttgtt cacaagtttc acaggcatca aggaatgttt caccaatcac ttcacttgca
     2821 taggcagcag cctcgcttgg atttttccat tccgtacaaa gcataccatc acagcttgca
     2881 ataggtatat ctcctttttc taatggtcct cgtcctaaag cgaggttata tccggctcca
     2941 acaatgtcag caacgatagc cattggtttt tgatttttac caccacgttg ttcgccacct
     3001 acccaaggga tgcctttttc agcatctttt ttatccattt tcttcttggc tgccattgca
     3061 tcaggctctg tttcagcaat ttcagcggct cggtgtaatc cggccatctt tgctaattta
     3121 ctgtccattg ctaaatcagc aagttggtta gacatggctt cgcaactggt tttgaagttg
     3181 ttgaagtcaa gttttccttg atatagacca ttagaaagaa tgtcatacag ttgaggattt
     3241 gctcgttgga taatcatcgc cgggaggctg gcaactgcac cggttgctga ctcaataata
     3301 ttgccataaa gatctttaaa accttcagta ataccattaa gctggttttt aatggaaagt
     3361 ttaatatcaa agttgccaca agttaaattc gatttccagc caatgccaat cccatattga
     3421 tttggtctgc cacgggaagc tggtgcggaa atggcactac ctcctccaat ggagtagtgc
     3481 atataatcag aaaggacagg gtagtattcc gtgagagcgt tattaccaga aacgcctaga
     3541 ttaccagtcc tatagcctgt tgtattttca ataaaattac taatgctatt gccaacatta
     3601 acggtattac tatttgcgta tccgctcatg gttgtgagca ttatacaagc ggttagaaat
     3661 ttgagacttt ttgctttttt catttattta tcctaatagt gctgataatt taatgagtat
     3721 gtgtactttt ccagtccaca tgacctaaaa aaacttgccc ttgtttttta cagcaagaat
     3781 agggcctcca taatgcccag ctgtaattct cagtattgga gaaactatct gaatgggtag
     3841 cccaatcacc actgtgcgga aagactttag ggtaggattc ggaagtaaat accttaactg
     3901 ttacatctga gggtttatta gcaggactag cacttgccat agctgaaagc agtagtagtg
     3961 gtaaatagta aaatgtctta gcttgcataa atgctccaca aatagagtaa ttcacgttta
     4021 ccttaaaaga tgatttggtt agaagaaatc ggaaattgtt ttttggtttt ggagagtttt
     4081 tgttagtata taagttaatg taacaaggga gagtggatgt atgagcaata gaatacctaa
     4141 agcctgttat gcttacccaa aatcgccgaa caacccgttt aatagattgt cggttatcaa
     4201 tctataccag ccgaaatgtc tcccagcatt ctgaaaggga actctgtaaa attacaggaa
     4261 tgccacctaa acaataaatg gataatgcca gccactttat ccgttgtatt ttgtcacatg
     4321 cgtgattgca atgtcataat attacccgac ttggggatat tatgacattg caatcattgc
     4381 tcacttttct tagcctgagc ccacaggtga ataaagggtg ccgtttgtgt cctcgatgat
     4441 gccacagata tgccgattaa agaccacaag gtaatcgggt aaattacgaa cgaaaatatc
     4501 aacattgtca cgaaggtgct gctattttca atatagtaac ttatattggt ctaacttaaa
     4561 tttcatttat tcttcaaaag tacaacaccg ttggtaagtg atttatcaac ggtgttgtca
     4621 tttccaattc tctaaaatca aaattcaatt ctcaattaac tttatgattc aatatggcta
     4681 aattatcctc aatttaagac gctgtatata acagcactta ataagtcgca taactcctga
     4741 gaagcgacgg ctatccttag aggtagttga gttattgcca aaatgtctcc cagcattctg
     4801 aaagggaact ctgtaaattt gcaggaatgc caactcaatc cctatcattt cagtgaatat
     4861 tcattgagat tccattttac ttccaccttg cggggaacat ttttcccgtg ttgggtaagg
     4921 atgtttctcc gcttttttac atttggagaa acaaactatg acaactcaaa cttctactca
     4981 aaaaaattac ttcaatctac atactaccgg cattggctat ttaaacgata ttcgtttagt
     5041 agagcataaa aaaggaaatc ctttttatgc ttgtcgtgtt gcagctcttg ttggcgaatc
     5101 tgactcacca gagtacagat attttgatat gaatgtagtc ggtaaagaaa ccgaaacact
     5161 catcaaaaaa tgccaaaaag cggtctctga agagaaaaaa gtgcttgtat catttgtaat
     5221 ggctgatttg tggttcgata ctttcactta ttcaagtgac agtaaatatc acaaaaaagg
     5281 cgatactgga gtgtctctta aaggtcgctt aatccgcatc aaaatgatta aaattgatgg
     5341 ggaaatcgtc tatcaagaaa aaccaaaagc agatgagcaa tctgagtaat tttacccaag
     5401 cccgacaatt tatgttgggc tttactttat gtcccgtagg gaaacactcc ctgcggtgtg
     5461 ttttccttta ttttacaatt ttaggagaaa cacgatgaac tcacttcaac aatttgtcaa
     5521 aacattatca ccaacagtcc ttaatggtcg ccattttgat ggtattgttg gcgatgttgt
     5581 ctttgttcag aacggctatg gtaaatggct tgatcaaggc tttactatta ttggttttga
     5641 caaaagcata gaggaaaagg attggattta cctagattgg gattgctatt ggttcggaaa
     5701 aaatccggca caggtaatcc ctgaaaaatc ttgggctcaa cgttcttggc ttgctagagc
     5761 gtattcaatc tctcttgaac gttttaattc gttaggctta gagcgtgaat ataacattgt
     5821 gtctgattgg gataacccca tttatggtac agatattcag ttaattgaac attccaagga
     5881 gtttttccgt tggactgaag ccgatgatag agggattttc ttagttcaca acgagatggc
     5941 ttttcatttc ttcaaaaaag aaattaaagc ctcatcggaa ccggaatact atcaacctat
     6001 tgagacattc tggaaagatg ctacggtggt attgaacata gaagatctag atatgttaga
     6061 gcgagtagga ttagagcaaa ctaatacacc tgctttagca aaattaacta cgtttgtaca
     6121 ggctgctcgc aacgaaattt ccaatggtaa tatcgtattc tatcgagcat tttaacatct
     6181 aatacaaaga ggattattat atgaatcgac aaggtattta tatcaacggt aaacaagtct
     6241 ttgactctaa aggcaaagag atcaacagcg ttcaaatcat taatagtaca gtgcaaacag
     6301 ttgttgggat aaataaaggt tatatctcaa tggctgccgg caataatgta ttaattgttg
     6361 atggacaaac aattgcatta gaaccctctc cgactatcaa cattgaagta tttggcaatg
     6421 taaccaacct caatgcaggt tcagaaaatg ttcgtgtaac aggcgatgcc aaatctgttt
     6481 caactgtcag tggtgatgtc acagtggatg gtaatgtaac ctcgaatgtg aaaactgtga
     6541 gtgctgacat tcattgtaaa tctattttag gtaaaattaa cacagtgagc ggtgatattt
     6601 atcgctaata ttgactatac accctatcgg gaaacattct cggtaggtat gtttccccac
     6661 tttttattta aaacttggag aaacataatg gaagattcct tttttgactt tgatgatatc
     6721 tcttgctatt taggacaatg ggaagccatc ttagaagaat atagcgatat tgtcagtatt
     6781 gaagactttt ggcttgttgc caaagaattt gaaactgtac cacatttcgg taatctgtat
     6841 caggagcttg tcatctctcg cttaattcaa cgattctgta ctgaattaga tattgagcaa
     6901 gattcagatt tagttgaatt tgactactat atcaatgcaa tagacacgca tttttacatt
     6961 aatcgccaaa gaatctgtga tatagatgat tggaatgaaa tgttagacaa gattcgtaaa
     7021 gaaatgacgc cggctaaatt agctgcctaa ttttttgcaa atttttacta aaaacgactc
     7081 ttgccgcagt cttagtccac ccacttgggg aaatactccc ctaatgggtg acatttcccc
     7141 gtattttatt taggagaaat gtatgacgtt tgaacttggt cgagtatgta caactcgtgc
     7201 tgttgctgaa tgtttcaatc aaaatgcctt ggttgaattg cttaatcgtt actgttatgg
     7261 tgattggggc aatttgtgtg aggaagataa attagcgaat caggaagcaa tgaaatacgg
     7321 agggagaatt gtagctcgct acgatgtaga tgatgaaagt ctttatatca tcacagaagc
     7381 tgatcgtagc tgtacaacaa tcttattttc ttatgaatac taaggagaaa tccgattaac
     7441 acagcacatt attgtgtttt caaacgccgt ctaatgatgg cgttttttta ttctctcaat
     7501 caacaacaga ataaatcatt caaaatcagc aatgacttcg ccatagttgg tctagattcc
     7561 tttcatactt ggagagatta aatgaaaaca aaaatatcct taatgactgt ttttatgctt
     7621 ggcttaagtg gttgtgttca tcaacaggat acagctcaaa atagtgatga aatcacacct
     7681 atgcctgatt cattgctaac acctcaaatg caacaattac ctccacaaga ccgtatcgtg
     7741 tccaaagata tttaccaaga acagactacc tcgaataccg aagtggtacg tgacggacgc
     7801 tacaccttaa tcagtattgc tccggaggaa ggtcaaaaat atctgcttga acaagtcgta
     7861 tcagtcaaag tctccaatcc taaaaatcgt tttactgcga atgtccagca aggtttgcgt
     7921 tctacattaa ctaatacggg tttagatttg tgttatccac ctatcggaga acaaggtaat
     7981 gcggttcaaa cactttttac ccgacctctt ccaaaagttc attaccaatt cggtccgatg
     8041 aaattatatg aagctctaca aatgattgca ggtcccgctt atgaactaac tcttaatgat
     8101 atatcccgta cgatttgctt taaagctcgg gaaagttcat tccaaaaccc tcataaacct
     8161 gctgttgccg ttacaacgac aacaattaaa accgaggtca ttgaggaata atgagggaaa
     8221 tatcaatgaa gtttaattta tgccgtaaag ctataccgtt ggcagtacta cctcttttat
     8281 tgtcctttgc attacctact ctggctcaaa atatgcaaac agagaccgta agtagctcac
     8341 aatcgacttt gcagaaacaa agccaacatc aacaatcaca attaagtgat agcctaaaac
     8401 aggctaaaga atggggattg accgttgaag aatggagtcg ttataccgaa ttaatgaaag
     8461 gtgagcgagg tatgtggtcg ccaaatctag atccgttaac tgctttaggt atcgaagcac
     8521 gaactgctga ggaaagggaa aaatatgctc gtatgttggc gaaaaaatat tatgaacgag
     8581 tgtcaaaaga acttgatttt gacaaagttt atcgtcagga gtttgagaaa ctttatccga
     8641 atgagctacc ttttgaggtt gaaccacata tttctcaatc ggtaggtcgt gtggtttatt
     8701 ttacccgttt tgataactgc gattcttgta aaagtgatct tgggaaaatc ctctcttacg
     8761 caaataatca aacacctctc gatatttata ttgttgggca aaacatctcc gataatgata
     8821 ttcgtaaatg ggctgccgac aataatattg acccaataaa agtcagtaaa agattgatta
     8881 cccttaacca cgataaaggc tattggctga gatattcgtt aggaaaaatg cccgctgctt
     8941 accaaatcaa acaggatggt gaatggacat cactcgtgta ttagtcattg ttgcagtctt
     9001 tctttccaac attagcattt ctcaggcaaa gcccttcaat ggatttggtt ctgaaattga
     9061 cagctatatt cgtatggctg ctcaacgtta tcaggtgagt gaagcaatgc tacgaggttt
     9121 tgtcaaaatg gaagatggct ggtatggcaa catttcacca acaggagcaa ccggtgttgg
     9181 gcagtttact atgggaacct ggaactggct atctgaaaca gagaggggaa agcaacttgg
     9241 tatgaagttg attacagcca aaaatcgggg ttcttacgcc gatccccgtc gagataaata
     9301 tacgaatacc ttagcaacgg cattattggc acgttggcat attgagcaat ttcaactccg
     9361 taatatccct attactgatg agaatttata tatggctcat aatatcggct tagatgggct
     9421 gcatcgagca atgatgggga aagctactaa agaagacatt ttaaatatgc gtcgtaatgg
     9481 aatgaaaagt tggatgacgg taagagactt tctcaattac caaaaaggac gttttttagc
     9541 acataagcaa gaagccaatt ttatgactgt ctccaagcaa gggcatattc ataccggagt
     9601 tgcagctttc gtttatccca caagccaaat gggaaaatcg gtcaaatggg ttgagccgga
     9661 taataaaatg atttgggttg aacctaaaaa tgcaatggtt tgggtagaac cccaaaatac
     9721 tcttcagagg taaaaatgca aaacaaggta aaagtattat tgtttagttt atatgtgtcc
     9781 catttctctt gggctgattt aaccgtgatt gctgatttcg gtggtgaaag tgcggtacgt
     9841 ttttatgaat ctttgcaacc ggaacacgat gaaaatgcac caaactatcc gaattccatt
     9901 tcagcctcag tgagtgaggc tgatatttta cccattgttt cccataaact atctcccggg
     9961 actattaaag aaaaaccatt tgatttaacc ggaatgcaag ccattttctt aattggagca
    10021 gataatacct cagctcagtg gttacaacaa aatcgagaga agttgatcac gttgaatgca
    10081 acagggcttg tagtgaatgt acaaacactc tccgaactta atcaattacg agacattgta
    10141 ccggaactga gaatattacc aactcccgga gatgaccttg cagagcgaat tggtattgca
    10201 cattaccctg tcttaatcac tgaaagcgga gtttcacaat aatgtctgct aaacatacct
    10261 tagaggcgtg gttacgacct cctgttgaat tttttccggc agcggttcac gcttcttgtg
    10321 cctttgtcag tgctgcagct ccttggtctt tggcactcca tccaagtatc ggttacagta
    10381 ttgcagctgg ttttggagca ttgagctttt atcgggcaaa acaaggttgg caaattgtgc
    10441 gttaccatcg caacctgaaa cgtttgccta attattcaat gacaagcaag caaattccgg
    10501 tcagtaataa gtatctgttt gtcggtaagg gatttgaatg gaaacctatt cacactcaac
    10561 ggttacacga ttgtttttcg gataacggtt caaaatatct ggagccttct aagctattta
    10621 aaatggctcg tgagtatgaa aaacgacacg aaaattggct aacacatctt acctcaaagg
    10681 atagccggtg gaatctggtt agacctctgc cacctgttga gggtattcct gctatccatg
    10741 gaattgagcc ggatgaggtt gatgtcgttc aatccgttag ctctcgggtt gggcatacgg
    10801 tagtattcgg tacaacacgt gtcggtaaaa ctcgtttagc tgaggtcttt gttacccagg
    10861 atatccaccg aggtaaaact gctgatgagc gtgaagtggt agtcttcttt gatccgaaag
    10921 gcgatgccga tatgctcaaa cgaatgtatg cggaggcaaa acgagcgggg cgagaagatg
    10981 agttttatgt tttccatcta ggttatcccg aaatttctgc ccgttataac ccagtaggac
    11041 gctttaatcg tatttcggaa gtcgctgggc gtatttcagg acagctaagt ggagcgggta
    11101 actcggcggc atttaaagag ttcgcttggc gatttgtcaa tatcgtcgca cgtgcattag
    11161 ttgaattggg aaatcgccca aactatgttc aaatttccca gtttgtacaa aatattgatt
    11221 cattattcct tgattatgcg gaaaaattca ttcgtaaaac cgatccaaaa atttgggaaa
    11281 atttagcaac catcgccgct ggactggatg aaaaagcact tcctttcgga atgaaagacc
    11341 gtccgctgat ttttgtgatc aacaagtaca ttgctgaaag tgcagtcttt gacccaattc
    11401 tacaaggact tgccagtgct gtacgctatg accgtaccta tttcgataaa attgttgcct
    11461 cattgttgcc gttattggaa aaattaacga cagggaaaat ttctgagctg ttatccccgg
    11521 attatgctga tattttggat gaacgtccga tttttgactg ggaatccatt attcgtaaac
    11581 gaggaatcgt ttatattggt ttagatgctc tctctgatgc gacagttgcg gccgctgtag
    11641 gaaacagtat gtttgctgac ttagtctcaa tggcaggtca tttatataaa tttgggacaa
    11701 atgacggatt accgggagca ttcaaagaaa aatccaaacc agtgaaaatt aatcttcatt
    11761 gtgatgaatt taatgaattg atgggtgatg aatttatccc tctcatcaac aaaggcggtg
    11821 gtgctggaat acaagtaact gcttatacac aaacactctc cgacattgaa gctcgtatcg
    11881 gcagtaaacc gaaagcggga caaaccttag gtaacttcaa tacactctat atgctgaggg
    11941 taaaagaacc ggctacggca gaattactca caaaacagtt acacgaagtg acgattaaac
    12001 gtagcacggt cacgtccagt acaactgact ccagtaatcc tgacgatgat aaggcgttta
    12061 gctccaatac cggacagcgt atcagcgaaa gtaccgttcc tatgttggtt ccggccaaca
    12121 ttaccaattt accaaaagga caagcattcg ctttaatgga cggtagtaaa ttatggaaaa
    12181 ttcgaatgcc gttacctgct gtcgataaag atgatatgat gcatcaatct ttacgagagc
    12241 tgacagatta tatgcgtaaa aactatcacg ttgttgaaaa ttggtggcaa ccggcattta
    12301 cagaatatac gccgtcccga gacattgcag aagcatttga agcgatgtcg gagcctcaat
    12361 ataaaacaca gatagaccat caagtagata atttcggcat agatgaacct tacgatattg
    12421 atgaagagga aaatgatgag tagtgccaaa atgagagagg aaaaaagaac caatctatta
    12481 gatttaccaa ataaataccg taattttaat ggggagtttt cagcttcctg tggcttagat
    12541 aatgcagaag aactcctgat tcatagtcaa tcctatttta ttgaatggtt tgaacaaggt
    12601 tattccttcc atcagtttgc cgaaaaattc gcagatcaag ggctttcgtt atggtctgcc
    12661 gatgaagtat ctatgcgtca ctcggataaa tccaaagata ttttcgcttt ttatcttgct
    12721 tttgataata atccttccgg ctatatcctt gtccagtgcc agctcgatag agaggatagt
    12781 ttgcaatgag tgaagaaact aaagctcgcc gaaaatcagg tttaatcaac ctgatttttg
    12841 gaaaaataaa tatgtcgctt gctgcaattg cagtatctct catattcagc attattttag
    12901 agtggatagg cattgctttc tggtggccgc aagaagggca tttgcatagc caaacgatga
    12961 tgctcaatga gatgcaatgg ctatcggatg acttttctca aagcctgttt gccaagtctt
    13021 ctactgcttt tgcaacatca attattgaaa acgtgtataa ctggttattc gtttatacgg
    13081 gtgttaagga gtggttaact tcaccgggta atggtagtat ggaacagtgg gtacatcact
    13141 acggcagagc ctatattgaa tctgttattt atgtggtgat cacctttatt attcgcctca
    13201 ttattattgt ttttaccagc ccactctttt tattgactgc actttccggt ttaaccgaag
    13261 gactgtcatt acgagatata cgaaaatttg gtgccgggcg ggaatcagcc tacctatacc
    13321 atcacgctag acgttatatt atgccaatga tggtcacagc ttgggtgctt tatttatcta
    13381 tcccgttttc aattcatccg aacatcattt tagtcccagc agcctgtttg tttggtttat
    13441 ccatctgtat aactgcggcg agtttcaaga aattcttgta aatttcataa gcccttagca
    13501 atatgcgggg gcttattctt ctgttgttgc ctctgtatct tcattttctg actcagtatc
    13561 ccaatcagaa tcctcccagt cttcgacttc ccaatattgt ggatattcat catcttcaat
    13621 gatttgttca gaaatttgtt cagaggtatt ccaaatagga aattccccgt actcatccaa
    13681 ttgtaactct atcttttttc gctcatcatc gcctagaaat tgccaataat tacagattaa
    13741 ctttcctctt aaacttaatt cccaaagacc tccgtaatta tattgagcat tttctgcatg
    13801 gcaaatattc cccactttct gtgctgaagg aagagaatgg gcaacatatg ggaaaactac
    13861 aaggaacagt gctagatatc tcatctggta tctcatcgta aagcgtgaag atttcggtgt
    13921 tgatactact taattgacta gcttaaacat agaataatat ctttctcacc atttactatt
    13981 ttgtgcaatt tctcttctga acggtaacag agttttccat taaattttag gatcgctttt
    14041 ggcactctta acctgaagta aaactaaaaa tagaggtgca ctatgtctat ttcaacatct
    14101 ttaaggaaag gtattgttgt ccttgttacc ttatcttgct tgccagcact tgcttcaaca
    14161 gaacaagagc aattagccca ggcaattaag caactggaag cggcaaaatt atcacttcaa
    14221 cgagcagaaa aagtagctaa agcctcgcct aaaacacgag aatactttaa ctattctgct
    14281 attcacagtg atattgaaac cgtcaaaaaa ggaattcaac aatatatcaa tccaagccga
    14341 gcattacctc gtgatccgaa tgtactacgt acattaagtg atgattatag caaattacgg
    14401 gggcagtgat gtcttactcc cctgcgaatg cgttttctgt tgccagcggt atcgaccctt
    14461 ttagattgaa aatcatgatc gccattattt ttctttcatt actgctctta gccgtcgctt
    14521 gggcattgaa tagtggttat aagggatttt catttagaga taaaagtcct ttttcacttt
    14581 tagttttatt tctcaaagga gtggcactgt tagcttgtgt tacttatttc tttatctatt
    14641 agattcgagg tctattatga accgcattaa atctcttacg aaacatctgc atagtcgtta
    14701 tttggctgta atgctcttct tctggggttc cctccaaaat gcatttgcag ccggtcctga
    14761 tgcttccaaa atcccagact tccaaattcc aggcgtggat agtaatacca aagatccaat
    14821 ggaaattatt tggattgttc tgaaaggtct tgtcactctc ggagctgtta ttttagcagc
    14881 cgtagccatt ttgacagttg ttaaagcatt gattggcatc tataacgaag ctacatctga
    14941 aaatggtaaa aaaggttggg gacactttat tctcgcctta attatcggct ttattgtgat
    15001 tttcttctgc ttgtggttag tcaaacttgc agtcggactc ttctaattaa aatgtctagg
    15061 agaccaatat gtcagaacaa gatacaacca ttccttttct tcccacccgc cttaatcgtg
    15121 aagctaccgt ttacggtggg atgacagtta gtgagttcgg catatctgcc ggattaggat
    15181 ttatgttagg tctgattgtt ggtctcttct tgtggatatt aactgatttt tggcttctta
    15241 ttccggcaat ggcaatgttg ctctgcatcg caactgtctt aatcggaaaa ggtattgtcg
    15301 ctgcggttaa acgaggaaaa ccagaggctt atttaaatag attagttgaa aagaaaatgg
    15361 atgacctctt taacggtcat aagtttataa aacgagaagg gttttggtct attcgccgat
    15421 atagaaggaa ataacaaatg agccagttaa aaagccaatt acacgaaaaa gataaacata
    15481 tcaaaacgac tcgaattttt gccttcctgt ttttcattat tattttggta ctggcaggcg
    15541 taatctatac tttcccaagt cgccttacga tatacaaccc accagatctg cgtgctggga
    15601 gccaacgagc ttggtgggaa attcctaaac caacaattta tgcattctcc tatcaaattt
    15661 tccagcaaat gaatcggtgg atgacaaatg gcgaagagga ttatgagaaa aatatcacca
    15721 ccctcaaacc atttttaaca ccacactgtg aagcattttt acgacaagat tataaagatc
    15781 gtctgcgtaa caatgaacta cgcagccgaa cccgtatggt gtatgaaatt accggtagag
    15841 gatatagtga agataaagtt accatattca gtgaagatag ttgggctgtc aatcttgatt
    15901 taagtgtgga tgaacatttc caaggacagc cggtaaaacg agtttatatt cgttttccta
    15961 tcagtattgt gagaatggat gtcgattctg ctcaaaaccc ttggggatta gggtttaatt
    16021 gctatcactc cattccaaaa ctcttagaag gtgttgagcc aaaaacagag gagaaaaaat
    16081 aatgaaacgt ctaccaattt tgctcgtctc tttgcttata ggcttctctg ggagtagctt
    16141 ggctgatgta ttgatgaaat gggaaagaaa accacttcct gttgcgttac aagtggaaaa
    16201 agaacgaatt atttttgtcg ataaaaatgt caaggttggt tatccggcag aactggacgg
    16261 taagctacga attcaatcta ccggtggagc cgtctattta aaagcattag aaacatttcc
    16321 ctccactcgc atagaattcc gtgatgttac aaccggtgaa atttttctat ttgatttaac
    16381 agcagcttcg aaagtcgcta atcctgaaga ggcaattcga gtggtttttg acggtacggt
    16441 agaaaaacaa tctttttcaa gcaatagaga tgatgaaagc tctgatctgc ctattgcgaa
    16501 tagccgtcct cagcttccag tacctgccgc attgacccgt tatgcagcac aatcacttta
    16561 tgctccacta cgtacagttg aacctttaga tggtgtgcgt cgtgtggcac atcgtctacc
    16621 taaaaaaatt accaagctgc taccggctta ttcaatagaa gcaacacctt taattagctg
    16681 gcagttagac gaatatgtag ttaccgctat tcgtttgcaa aataagggta acactcaaat
    16741 tacgcttgac cctcgagagc ttcaaggaca attttatgca gcgactttcc agcataattg
    16801 gcttgggggg tacggagaaa atgaagatac cacaaccctg tatttagtga ccgaaggcta
    16861 tgctaataac gcattaattc cggaaacccg tccagtcaaa ctagtcagta aaaaagtgaa
    16921 aagaacaaca aaagttgaaa ctaaaccggc tcaatcggcg aagtaggagg aaaggcaatg
    16981 aaagcgaata aagccttatt tgtcatactg ggcattgtat ttatcttagt cggtggaatt
    17041 gtctatgcct tatttggacg tagtgagtca gttccacaac cgacacaaca ggaaatttta
    17101 gatatttcca ttaaagattt aacacctgaa caagtcagaa caatgggtat tcaaggtgat
    17161 acgccgattg atacgctgcg tacccttgtg acaaccgtta aatccaatga gcgtcgtatg
    17221 gatgagttaa ttgatgaaaa tcgccaactc aaacagaaag aagatttagt gaatggtcgt
    17281 atcaatgatg cggtaaataa tgaaacccga aaactgcaag agcagattgc agatttgaaa
    17341 atgcaattat ttgatatggc gactctcgtt aataacaaac caacaacaca agagtctacc
    17401 cctcaggtag aggctcagcc taatacggta ccgaataaag gatttggaac agcaggagat
    17461 aaattaccta tcggtggtgc aactatgtcc aacgtggata ccagtggaat gtattgggta
    17521 gaaccaagcg atcagattag tttggatgca agtggaaaac ctgtgtcagg caatacagta
    17581 gcaactaaat ttggctttcc gaaagatttt aatggaggac aatctgcagg aaataacgaa
    17641 aagaaaggtc tgccgttttt cccggaaaca gatactgtga caagctcaca aggaacggct
    17701 ggaaacgcac gtggtaaaaa ccagatgaca tcagtatata ccataccaag taattcaaca
    17761 ttccttgatt cagttgcaat gacgggactc atcggacgta tcccgattga taatcgagta
    17821 tcggatgctt atccgttcaa agtacttatt ggtcgagaaa atctcattgc taatggtatt
    17881 gaattacctg atgtagacgg agcaattgta tcaggtactg ctactggcga ctggacacta
    17941 tcctgtgtta gaggcagtgt aaaaagtatg acttttgtgt tcaatgatgg acgtattgtg
    18001 agtacctcta ataaagggaa tggcgaagag attggctggt tatcagatag ttcgggtatc
    18061 ccttgtgttc caggagagcg gaaaaccaat gccccagaat atttgggaac gaatttcttt
    18121 ttggctgctg caagtgctgc tgcccaagga ttctcacagt cacaaacaac cactgttgtt
    18181 gatggaagta gtgttgttgg agctgttacc ggcgatcagg gtaagttcat cttaggacaa
    18241 gctatcggag gcggcttaaa agaatctgct gactggttta gaaaacgttt tgctgaaaac
    18301 tttgatgctg tatatgtgcc accgggtcat cgtgtctcta ttcatatcga gaaagagatt
    18361 gaaatcgatt acaacgccca aggtcgtaaa gtgaaatatg gctcaaaatc aacgaattca
    18421 caactggatt agaaggaaca aacgatgaaa catttatttt gcataatgat aagtgcctta
    18481 ttgataacag gttgcacaac atcaacagaa gaattattac ctactaacgg cagtacgatg
    18541 cgggatattt gggataaagg agcgagtaat aacgatctac acttataccg ttcacaacaa
    18601 gggcgaacac ttgaccctgt cgattatatt cctaatagtg agcaacgagc ctatacccgt
    18661 acggcagaaa atgaaattaa taacttgttc cctcgtttac ctaatccgga tctgattatg
    18721 tatgtgtacc cacatcaaac tcgttccgga gagcctctca gtattccagg atattctaca
    18781 gtactacctt tctatggacg agttcaatat gctcagccgg gtgatagaac aagagggctt
    18841 taatcatgtc ttttttaagt gaggtatttt cctctttcaa accgactaaa gcaggggtta
    18901 tatcaagtga accaaatgaa aataccggtg agtctgacat cattcaaaat aaaatgagtg
    18961 aggtaaataa cgaggttcaa caattaaaac gcagtggaaa actcacccag tcacagcatc
    19021 accgtcggtt atactcacaa gagccctcct ttatcgatca tctaccttgg ggagaatatt
    19081 tgaatgagca cggtgtgata ctccttgatg atgcccgtag tgttggggca gtatttgata
    19141 ttaagccgat tggtactgct ggccgtagta ttgaattttt aagtcggata cgtaatcttg
    19201 tcaaagatgc cttacaagat agctttgatg agctggatat aaatccttac gtcgtgcaat
    19261 tttattgcca agatagcgat gatttacagc cttatattga acagctacgg aattatatct
    19321 cgcctattgc ccaaaatagt gcatttagcg aagaatggtt acgagtacaa gccaagcatt
    19381 tcaaggatat tggtaaacca gaagggcttt ttattgatga cagagtgaca aatacagctt
    19441 ggtctggacg tattcggagt accaaaatgg tgatttatcg atatgtagat aagtccaatg
    19501 attccaatga aaatcttatt attgagcagc ttaataatgc ttgttcccga atcaatgggg
    19561 ctctaacaac ggctggatta cagttacagc ggctagatgg tgaacaaatt cgccaatggt
    19621 tattgcgttg gtttaatcct aatccatcta ttaattttga gggattaagc aacaatgatt
    19681 gttaccatct ttttgg
//
LOCUS       DANLUA010000021        19661 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLUA010000021 DANLUA010000000
VERSION     DANLUA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19661
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            141..389
                     /gene="rpsP"
                     /locus_tag="PXB44_000603"
     CDS             141..389
                     /gene="rpsP"
                     /locus_tag="PXB44_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL5281114.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            422..949
                     /gene="rimM"
                     /locus_tag="PXB44_000604"
     CDS             422..949
                     /gene="rimM"
                     /locus_tag="PXB44_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL5281115.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            994..1749
                     /gene="trmD"
                     /locus_tag="PXB44_000605"
     CDS             994..1749
                     /gene="trmD"
                     /locus_tag="PXB44_000605"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL5281116.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            1776..2126
                     /gene="rplS"
                     /locus_tag="PXB44_000606"
     CDS             1776..2126
                     /gene="rplS"
                     /locus_tag="PXB44_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL5281117.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            2282..2638
                     /locus_tag="PXB44_000607"
     CDS             2282..2638
                     /locus_tag="PXB44_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281118.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            2640..2963
                     /locus_tag="PXB44_000608"
     CDS             2640..2963
                     /locus_tag="PXB44_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281119.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(2973..3305)
                     /locus_tag="PXB44_000609"
     CDS             complement(2973..3305)
                     /locus_tag="PXB44_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5281120.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(3398..4594)
                     /locus_tag="PXB44_000610"
     CDS             complement(3398..4594)
                     /locus_tag="PXB44_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL5281121.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(4743..6086)
                     /gene="accC"
                     /locus_tag="PXB44_000611"
     CDS             complement(4743..6086)
                     /gene="accC"
                     /locus_tag="PXB44_000611"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL5281122.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(6158..6625)
                     /locus_tag="PXB44_000612"
     CDS             complement(6158..6625)
                     /locus_tag="PXB44_000612"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL5281123.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(6878..7660)
                     /locus_tag="PXB44_000613"
     CDS             complement(6878..7660)
                     /locus_tag="PXB44_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5281124.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(7670..8107)
                     /gene="aroQ"
                     /locus_tag="PXB44_000614"
     CDS             complement(7670..8107)
                     /gene="aroQ"
                     /locus_tag="PXB44_000614"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL5281125.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(8193..8561)
                     /locus_tag="PXB44_000615"
     CDS             complement(8193..8561)
                     /locus_tag="PXB44_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5281126.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(8621..9445)
                     /gene="dapD"
                     /locus_tag="PXB44_000616"
     CDS             complement(8621..9445)
                     /gene="dapD"
                     /locus_tag="PXB44_000616"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL5281127.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            9593..10255
                     /locus_tag="PXB44_000617"
     CDS             9593..10255
                     /locus_tag="PXB44_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL5281128.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            10252..11004
                     /locus_tag="PXB44_000618"
     CDS             10252..11004
                     /locus_tag="PXB44_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5281129.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            11095..11931
                     /gene="ftsN"
                     /locus_tag="PXB44_000619"
     CDS             11095..11931
                     /gene="ftsN"
                     /locus_tag="PXB44_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL5281130.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(11973..13154)
                     /locus_tag="PXB44_000620"
     CDS             complement(11973..13154)
                     /locus_tag="PXB44_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5281131.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            13261..13599
                     /locus_tag="PXB44_000621"
     CDS             13261..13599
                     /locus_tag="PXB44_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281132.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            13731..14600
                     /locus_tag="PXB44_000622"
     CDS             13731..14600
                     /locus_tag="PXB44_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL5281133.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            14692..16503
                     /locus_tag="PXB44_000623"
     CDS             14692..16503
                     /locus_tag="PXB44_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281134.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVAEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
     gene            16512..19292
                     /locus_tag="PXB44_000624"
     CDS             16512..19292
                     /locus_tag="PXB44_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL5281135.1"
                     /translation="MNAEQRVIVTPTFWGKFFGKIKSVELQQNKVIVTDKKNNITEHD
                     LGKTFDFPAIQKSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKVKVK
                     EGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQYISS
                     LFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLAAAG
                     TGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQERAREANLDLENSPS
                     ISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQLAY
                     QPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPRYVS
                     KRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAEHNT
                     TLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRYLKC
                     LQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDMILL
                     ATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSIYRF
                     SGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTHTKV
                     ADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDAKSV
                     IKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFPHSE
                     ERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFKCNE
                     CSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGYGIK
                     GDQCTNTRKYFM"
     gene            19314..>19661
                     /locus_tag="PXB44_000625"
     CDS             19314..>19661
                     /locus_tag="PXB44_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5281136.1"
                     /translation="MDLFESKKQLIELIKKYDSDKEIYSSSSYNESQLRTDFLDPFFG
                     LLGWDITNSKNKLTSEREVIVEEPLKEKVSENVKKPDYTFRFNSQRKFFVEAKKPSVK
                     IKTDPLPAHQVRRY"
BASE COUNT         5936 a         3966 c         4175 g         5584 t
ORIGIN      
        1 agtcatatca atgaatctcc cttgtctttc cgcaaaaaat ctgtataatt tgaccgcttg
       61 ttttctcttt tgaaacaagt tcgttaccct cacctcgaac gagggtttat tttttactat
      121 caacatttag aggaaaggtt atggtaacca ttcgtttaac tcgtggcgga gctaaaaaac
      181 gcccatttta ccaaatcgtg gtagtggaca gccgttcacc tcgtgatggt cgctttatcg
      241 agcgtatcgg tttcttcaac ccattagcag caggtcaagc tgagcgttta cgcttagatg
      301 tggctaaagt agatgcttgg gttgctaaag gtgctgattt atcagaccgt gttgcttctt
      361 tagtaaaaga agctcgcaaa gcagcttaat ttttaagcca aaattaaata agtaggtgag
      421 tatgagcgaa caaaagatcg aagttgtcgg aaaattaggt tcaacctatg gtatccgtgg
      481 ttggttgcgt ctctattcat cgacagaaca aactgagagc attttcgact accagccgtg
      541 gtttttaaaa attaaaggtc agtggcagca ggtcgaatta gaaagctggc gttatcatag
      601 caatgatttg attgttaaat tgaaaggcac tgaagaccgt gaagccgcac agttattaac
      661 caacgctgaa attggcgtgg atttgtctgt gtttcctgaa ttggaagagg gagattacta
      721 ttggcacgat ttgatcggct gttcggtagt aaacttagag ggctacacaa tgggaacagt
      781 tgccgaaatg atggaaacag gttcaaatga tgtgcttgtg gtgcgtaata acggcaaaga
      841 tgctttcggt aaacaagaac gattaattcc gtttttgtat gaacaagtag ttaaaagagt
      901 agatctcgcc accaaaacca ttacggtgga gtgggacgct ggtttctaac ctcaggtatg
      961 cggtgagctt tggaaaacat ttaaggcgtt aaaatgtgga ttggtattat ttcactgttt
     1021 cctgaaatgt ttaaagcaat taccgatttc ggggtgacag gacgtgcagt taaacaaaat
     1081 ctactgcaag ttgaatgttg gaatcctcgt gattttacat tcgataaaca taaaactgta
     1141 gatgatcgcc cttatggcgg tggtccggga atgttgatga tggtgcaacc tttgcgtgat
     1201 gcgattcacg cagcgaaaaa ggcggcatta gcagtagaag gcggtgaagc taaagtgatt
     1261 tatctttcgc cgcaaggacg taaactcgat caaaacggtg tgcaaacact ggcttcaaat
     1321 cagaaactga ttttaatttg tggacgatat gaaggcgtgg acgagcgatt gattcaaaca
     1381 gaagttgatg aagaatggtc aatcggtgat tacgttctca caggggggga attacctgca
     1441 atgacattaa ttgatgcagt tgcaaggttt gttcccgggg tattaggcaa acaggcttcc
     1501 gcgttagagg attctttcgc agagggatta ttagattgcc cacattatac ccgccctgaa
     1561 gtattagacg gtatgcctgt tccggaagtg ctgatgtctg gacaccatga acaaattcgt
     1621 aaatggcggt tggaacaatc gctggaacga acgtggttaa gacgccctga gctattggat
     1681 agcctagctc tgactgacga acagcgtgtg ttgttagcta aaattaaaaa acagcacaaa
     1741 atcagttaat tctaggatta tagaaggttt ttaaaatgag taacatcatc aaacaacttg
     1801 aacaagaaca gttaaaacaa gacttaccta gcttccgtcc gggtgacaca ttagaagtta
     1861 aggtatgggt agtagaaggt agtaaaaaac gtttgcaagc gttcgaaggc gtggttattg
     1921 caattcgtaa ccgtggcttg cactctgcat tcactctacg taaagtttca aatggcgtag
     1981 gcgttgagcg tgtattccaa actcactcac ctgtaattga cagcatttct gttaaacgta
     2041 aaggtgcggt acgtaaagct aaactttact acttacgtga gcgttctggt aaatctgcac
     2101 gtatcaaaga gcgtcttggc gactaattgc tacaaaacag tgaaatgcac aaaagccttg
     2161 aggaaactca aggcttttgc tttttgtact tacaagcagt tatttttcct caatgattta
     2221 caaatcttgc tttctttttt gttataatgt taacaagata acattaatga taggtgtcga
     2281 tatgaaacaa ttaagtatta gattgccaga cgatgaattt acttttatta aaacatttgc
     2341 ttctgctcat aaactgactt tacaaaattt tgtgatgtcc gcaatccaag ctaaagttgc
     2401 agaaatgtct gctaaaaata aaatatctga agaaagtaac attcatcaac ctctttctaa
     2461 agatgaaaaa ataatgctaa atgaggcaaa agtattttat tctgctcgtt cgcatttaaa
     2521 ggcagaaaat gtgattcttc cgagtgattt tatggagcaa attaaatcag gctgtggagc
     2581 agaaaaaatg gcagaaatga tgggatatac caaagaagat tgggagtcaa tgaagtaata
     2641 tggggcagat tacatttagt aatattttca caaaagtgat ttttgagaat gtgctttctg
     2701 cttcaactaa agagaaaata aaacaaatgc tttttcagtc tgtggttgca ttacctcctc
     2761 tccattcaga aagaaaaatg attttacagt tgaaaaagca aaaaattacc attttttatc
     2821 aagttattga ggaacaatct gtgcaaatcc tcgcttatca atttggtgga actgataaat
     2881 tgacaggtgt gtctaaagct cactttaaaa cactagatgc catttttcaa atgacggatg
     2941 aagagaaaga aggaaaattc taatgtaagc ggttattttt ccatattttt ttgcaaataa
     3001 cgtttctcaa attttgcttg atggcgttca agcacttccg tcatcgaaat gccatattta
     3061 tcggctaata caaaaagatt atccatcaca tcgcctaact cttcgattaa gttatctcgt
     3121 ttttgctgtt gtgaagcttc ggtttcgtcc ggtcgttcac gcccgatttc aatcgctctt
     3181 actgcacaag ccacttcgcc gatttcttct gctagataag tcacccgcat aaaagggctt
     3241 cgttcatacc aaccttgttg ttggtaaaac tggcgaaccc attgttgata ttgttcaatt
     3301 tgcattttat ttctgattca aaaagtaggg acgaaaaatt tttcgccctt ttataagaaa
     3361 atgtatcagg gcaaatgatg atttgcccct aggagtgtta ctctgcctca gagagaataa
     3421 tccagccact atccagccag tcgcaaatgg tgtctagcat tagctccagc tccgcttggt
     3481 cttgcacttt ggaggcaagt ttgttccacg aaatcgaatc tccattggcg atacgaatca
     3541 gtaatgccat ttctgcttcg tttaactcat caatccactc gccatttaca taaacccgtt
     3601 gagggttctc ggtgtagagg atttttacat ttgcatcttg ctgtaaccag ccgccggctt
     3661 cgagaatttc ttgcacttcg tcagggtaat attcattgtc agtttgcaat aattcataac
     3721 ggcgggagct tgccgcagtg gcaacactgt gagtgagtaa ctcatcgaac tgttcggaat
     3781 tttggagcaa gtcgattaac tgatgtttaa acattttcac cattttcgga tcaagcaacg
     3841 cattcggctg ttcattcggc gttaagcgga acggaagatg aaattccgat gaattaatgt
     3901 tgtcggcttg ttgttcgatg gttttgcaga gattttccag taaatcggtc gcatttggat
     3961 aacgtaaacc aaaagagaaa gttaaacctt gctcgctttc ggaaacacca tagtgagcca
     4021 ttcgagacgg cacataaagc acatcacccg ggttcattac ctcgtccatt accagttcgc
     4081 ccatatcatc aaaaatgcgg atcggttggt ttggtttgaa ttcggtgctt ggatcgcacc
     4141 atttgcctaa ttgccaacga cgttgcccat agccttgcac caagaacaca tcgtactcat
     4201 cataatgttt gccgacagtg ccgttgagcg gtgagcaaga aaccataatg tcatcacgtt
     4261 gccattgcgg gataaaacca aaagcctgcc aaagctcgcc tagctcagga gaccattgct
     4321 cgaggttttg caccattaca gaccattgta caggtaactc ttccaaatct tcctcaacaa
     4381 gcgggctgga tttaacagac catttctcgt tttcgcattg aatcaggcgt gcagtgactt
     4441 cttcctctaa tgccagttct aaaatatctt ccggctcaaa caatccgaca atttggggta
     4501 agccgttacg aattaatagc ggtcgttttt gccaatattc tcgcaaaaag gtttcagggc
     4561 tgatattttc aggtaagcag aatggaatgt tcataatggc tccttgctac gtgcttcttt
     4621 gagattactg atattctacc cgagaaatca ggaaaaacgg gaaatttttt atgatttagc
     4681 gacatcattc acattttgaa gaggaaataa tgaaaaaggc gaacattgtt cgcctttgaa
     4741 atttatttta aaccgagttt tttctcgaga tagtggatat tggtgccacc tttgcggaat
     4801 tctttatccg caagaatttg agtatgtaaa ggaatattgg ttttaatgcc ttccacaatg
     4861 gtttcggaaa gggcattttc catccggcga atcgcaatat cacgattttc agcaaaggta
     4921 atcaatttac caatcattga atcgtaatgt ggcggcacgc tatagcctga gtaaatatgt
     4981 gaatcccaac gcacacctaa accacccgga acgtgtaaac gggtaatttt acccggcgat
     5041 ggcaagaagg tgtttggatc ttctgcgttg atacggcact caatggcgtg acctttcact
     5101 ttaatatcat cttgtgtgat cgaaagtggt aagccggctg ccacacgcag ttgctctttt
     5161 accaagtcca caccggtaat catttcggtt acagggtgtt ctacctgtaa gcgggtgttc
     5221 atttcaatga agtaaaactc gccgttttca tataagaact caaaagtacc tgcaccacgg
     5281 tagccaattt cacggcaggc attggcacaa cgctccccaa taaattgacg caattcaggt
     5341 gtaataccct gagcaggtgc ttcttccacc actttttggt ggcgacgttg cattgaacag
     5401 tcacgctctg ctaaataaac cgcattaccg tgagtatcgg caagtacttg gatttcaata
     5461 tggcgtggat tttctaaata tttctccata tacaccatat cgttattaaa tgccgctttc
     5521 gcttcggctt tcgtcattgc gatagattct tccaaatctt tctcgtgatg aaccacacgc
     5581 ataccacgac cgccaccacc acctgatgct ttgatgatca ccgggtagcc gatacgttta
     5641 gcgatttctt tatttttctt cggatcgtta ccaaccggac cgtcagaacc cggtacgcaa
     5701 ggcacgccgg cttttttcat tgcattgata gcagagactt tatcgcccat taagcgaatc
     5761 acatcagccg ttggaccaat aaagataaag cctgatttct caacttgttc ggcaaagttt
     5821 gcgttttcag ataagaaacc gtagcccgga tgaatagcat cggcattcgt tacttcggca
     5881 gcggcaataa tcatcgggat atttaaatag ctttttaccg atggagcagg accgatacaa
     5941 atggtttcat cggctaattt aacgtgtttt aattcacggt cggcagttga atgcactgca
     6001 accgttttaa tgcccaattc tttacaagca cgcaaaatac gcagggcaat ttcaccacgg
     6061 tttgcgataa caactttttc taacataggg aaattccttg tgttctagaa aatgtagggc
     6121 gtgttgcaca cgcccgttgg ataatttaag gaagagctta ttcaatgatg aataattttt
     6181 ggtcgaattc taccggttcg ccatcattaa ctaagatttc ttttaccaca ccggctttgt
     6241 ctgactcgat acggttcatc attttcattg cttctacaat acataacgta tcaccgattt
     6301 tcacggtttg acccacttca acgaacggtt ttgcatctgg gcttgggcta cggtagaaag
     6361 tccccaccat tggtgaaagc actgcgtggc cactcgtttc tgcggctttt ggtgcttcag
     6421 ccggagctgc aacaggtact gtcgaagcta cttgaggtgc cgctggtgca acaacaggag
     6481 ctgctgcata ttgaactgca gctggtgcaa cagctggagc agcacggcta atacgcaccg
     6541 taccttcttc ttcagacact tctaactcgg taatgccaga ttcttcaact aattcaataa
     6601 gttttttgat tttgcgaata tccataataa gattccgtat gtgattaaat aaaataaaaa
     6661 agagaactcc acatttgggg tataaattca aatgtagagt taaaaaatgc tgggcgtaga
     6721 atacctgaat ttcgctaatt ttgcgaataa aatctgataa ttttggcgaa aatggacgat
     6781 tttagcattc atcgtaccag ttaggatttt tggaaaatgg tgactcacaa gcgattgaaa
     6841 attgccaatt ttttgtaaaa aacaaccgct tgtcagatta tttcaataaa tcttcatttt
     6901 tcttgtaatt ccacttgcta ccgggcttgt cgatatactg aaaatattcg ttggtaaaag
     6961 taccttgagc acaagttact ttgtctcggc ggaaatttac ttctttatca ccaatggcat
     7021 tatttagggt gtcaggaccg gtcataacaa acacgccacc ttcaatccga cgattctcaa
     7081 tattgctgat aatcagctca agggtgtctt ttagaatcca atttccagct tccgaagcca
     7141 agaaaaagtt ggtgtattta tcacggcgtt taattaacac ttcgctatca ttttcgccaa
     7201 taattttaga gagtgggtaa accaaatgcc cgtcaatatc aatataaatg ccaccttctc
     7261 ggagcaaagt aaaaattcgc caaaaatcag cttgtgccgc accatcggtt aatttgctat
     7321 aggcgttaaa ggtgcgttca tcggcgttgg ccttgatgta tgcctctcgt tcttctgtgc
     7381 taacgtagcg ataatcgtag ctcaatgaca ttaggcggtt aaacagataa ttcgcataca
     7441 ttggcaaggt gactttgtta gaataattcg tttgccaaat ggttttggta attttgctcg
     7501 gaatgcttga ttggattttt gccgggctat attccggaat ggtgaaacgt tttttcggaa
     7561 aaagtgcatg gaacggataa ctcaatattt tgacgatatt accgaataaa cggaagactc
     7621 cgttacagat cacaataaat tgttcatttt taacagccat tttattacct tatgctttta
     7681 agtgtgaaat tgcaaaatcc aacgcatatt catagccttt tgcccctaaa ccgcaaataa
     7741 cgccttttgc aatatcactt agataggagt gatggcgaaa aggttcacgg ctatgaacgt
     7801 tagacaaatg cacctcaaca aacggaatcg acacactcaa taacgcatca cgaattgcca
     7861 cgctggtgtg agtaaatgcc ccggggttaa taataataaa atcggtatgc tgaaaggctt
     7921 ggtgaatacg gttgataatc ggctcttcac cgttggcttg aaagctctcc agctcaacat
     7981 cttgggccgc agccaatttt tttaagtggt tttcaatatc cgccaaagtt tgcgaaccgt
     8041 aaatttgtgg ctcacgtttg cccaacatat ttaaattcgg gccgttaagg agaaggattt
     8101 ttttcatatt tacttattca acttgtaatt cataattaag ccattcacgg ttttctttcg
     8161 cccccaagcg ttgataaaaa cgaataccac cgtcattcca aggattttat tgtcttacaa
     8221 tgatgtgcct tcgcctcatt tttcaaggct tgcattaacg cctctcccac tttctgattc
     8281 cgagcagttt catcaacata aagctctttc atataaatag cggggcggtt ttgggcagtg
     8341 taaggaagaa aataataaac caatatgccg ataatgttag acgcattttc agccactaaa
     8401 cagtaaaaat cgggtgggct tttgcgaaag ccggattcca ttacaacttc aggagtaatc
     8461 gcaaaagaat cgatatagtg ctcaaacaca gccagttttt gcatcagagg gaacacttgg
     8521 tgggtatctt gttcgaccat tttgcggatt gtaaatgcca taaaattcct tacaagcggt
     8581 aaaaaaatcc ctcacttttg caaagcgagg gaggttttaa ttattcgtca atagaacgta
     8641 ataagtcgtt taagcccact ttgcttaagg ttttctcatc cacttttttc acaatcaccg
     8701 cacagtagag gttgtgtgaa ccatcttttg atggaagcga gcctgaaacc acaacagagc
     8761 ctgccggcac acggccgtaa tgtacttcgc cggtttcacg gtcgtaaatt ttggtagatt
     8821 gaccgataaa cacgcccatt gagatcacgc agttatcttc gacaatcacg ccttctacga
     8881 tttcagaacg tgcaccgata aagcagttgt cgccgatgat ggttgggttc gcttgtaatg
     8941 gctctaacac accaccgatg cccacgccac cagaaaggtg aacattttta ccgatttgcg
     9001 cacaagaacc cactgttacc caagtgtcca ccattgtgcc ttcgccaacg tatgcaccga
     9061 tgttcacgaa agatggcatt aacaccacgt ttttctcgat atgtgaacct ttacgcacca
     9121 ccgcacccgg cacggcacga atgccatctg ctttgaattg ctcttcggtg tagttgccgt
     9181 atttggttgg cactttgtcg tagaatttgg tttctgcacc gtcgatgatt tcattgtcgg
     9241 caatacggaa tgaaagtaat accgcttttt tcacccattg gtttactacc cactcgccat
     9301 cttttttctc ggcaacacgc agtgaaccgt tgtctaatcc tgcgatcacc tcttctactg
     9361 ctgctttggt ttcagcatca acggttttcg gagtaatttc cgcacggcgt tcaaatgccg
     9421 cttcaataat agcttgtaaa gacattgttg tgttccttca atatataaat taaaacagaa
     9481 aattcggctc actttttaac atagaaaggc gtttatttcc acaagaaaaa acatatcgca
     9541 gaaatttagt ttataattcc aagccactta tttgatacaa gcggtgattt ttgtggaaaa
     9601 ttttgcaaat ctgcttaccg gtaaaacgcg tgagcattta gtgccgttgc cgaacgctct
     9661 ttcggataaa cattaccttc agcctcaggt ggttgaggct tttttgacgt tgcagcatgc
     9721 tgcaaaacaa gccggcttta atttacagcc tgccagtact tttcgtgatt ttgaacgcca
     9781 aaaagggatt tggaatgcga agtttagggg tgagcgtaag gttcacgatg ataacggggt
     9841 tgcgattgat atggcggcgt tatcggatct tgaaaagtgc aaagcaatgt tgagatggtc
     9901 tgcggtgccg ggggcaagtc ggcatcattg gggaacggaa attgatattt ttgaccctga
     9961 tttattgccg gaaaatacgc ctttattgct tgagccgtgg gaatatttgg aaggtggcta
    10021 ttttgccgat ttaaccaagt ggttacacgt taatgcggaa acatttggct tttatttccc
    10081 gtttgacggt aaacattctc gagtgggctt tgagccgtgg cacattagct atcgcccgat
    10141 ttcaaatgaa tatgagcaac gctttaacga tgaaatctta aaagctgcgt ggcaagatga
    10201 agaagtggcg ggtaaggaat gcttacttga acattttgaa gaattactaa aatgatccaa
    10261 ttaattaacg aaacccaaca acacgacaaa ttccgccaaa tttgcgacaa atggcagctg
    10321 gttgaagata aatccagccc gttagcctta gtgctaacgg aggaacgatt ggaactacgc
    10381 aaattagacg aacccaagct cggggcgatt gcagtgaatt ttgtggacgg tacgatggca
    10441 catcggcgta aattcggtgg tgggcgaggt gaagccgttg cgaaagcggt cggcattaaa
    10501 ggggattatt tgccaactgt gattgatgcg acagccggtt tagggcgaga tgcttttgta
    10561 ttagcagcgg ttggttgtaa agtgctgtta gtggaacgca atccaattgt cgctgccttg
    10621 ttggaagatg gtttaaaccg agcttatcaa gatgtggaaa tcggcagttt tatgcaagaa
    10681 agaatgatct tagccgatgt tcgagacatt tctcagctag atccacaatc ccaaatggct
    10741 gaggtagtgt atttagatcc gatgtatccg cacaaacaaa aaagtgcgtt ggtgaaaaaa
    10801 gaaatgcggg tgtttcaaca tttagttggg gcagatttag attcggacag ctttttctta
    10861 ccggcaaaag gattggcaaa aaaacgtgtg gtagtgaagc gtccggacta tgccccgttt
    10921 ttagcggagc aaaagcctga tttttcacac accaccaaaa atcaccgttt tgatgtttat
    10981 ctttctcacc tgaaaaatga ttaaaggtgg gtcatgcgat aggcaagcgg ttatttttac
    11041 agtaaaattt acaaattatg ttatcgttta tttatattta aaaaggcggt tatcgtgcca
    11101 caacgtgatt atgtcgctcg ttccaacgag aaaaaaagta aaaaaaatca aaaagaaagt
    11161 aatgttaaaa agattttaat gcggattatc ggggtaattt tattcttcgg tattggttta
    11221 tggtttttga aatccagtgc acctgtacca acagttccgt cagcaccgca gcctgctaat
    11281 tcacagacta aaagcgaatt accaagtcga ccggaagaga cgtggagcta tattcgtgat
    11341 ttagaaaccc gtacggtaat taccgataat agccaaactt cacaagaacg tttggcacaa
    11401 ttaacggcgg aacaaaagca gcaaattgaa gaaagacgca aacaagaaga agcacgctta
    11461 gccgctgagc aagctcaaca aaagcctgcg accgatgtat ctgctacaga attggctccg
    11521 aaaacagagg aagttaagtc gttaacggaa gaagaattag cagcaaaaaa agcacaggag
    11581 caagctttag cacaaaagca agcggaagag aaacgcagac aagcagagct taaaaaacag
    11641 caggaagcga aaaaagagca gccggctaaa attgtagcaa gtgctgcaac actggcagat
    11701 aaagctgcaa atcaggcggg taaatttggg ttacagtgcg gtgcatttaa aaataaagcc
    11761 caagccgaaa atatgcaagc aagattggca atggcaggtt ttaacgcccg tatcgcaagc
    11821 agtgccgatt ggaaccgtgt ttttgtcggc ccgataggtg acagagcggc aacttccaga
    11881 gctcaatcca atgcacgcag tgtagcggaa tgtgtgattg tggcgatgta aaattgattg
    11941 aaatattgaa gagagctata ggctctcttt ttttatatcc caatcgcata tttcaaggct
    12001 tgctttttga tgggtgacac tttatcagcc aatactaaac cgacattgcg ggcaattttg
    12061 agcggtaata aatcggtctt aaacgcttta taaaacacgt ccatgcctgt ttgcatcagt
    12121 aaattatccg gtttacgttt ttgctcatag cgttgaagca cctgctcact tgcaaaattt
    12181 ttgccttttt tgaccgcttg ttcgatcacc tcaagcaagg ttttaacatc tttaaagcct
    12241 aaatttacac cttgtccggc aagcggatta atggtgtgag ccgcatcgcc gactaatacc
    12301 accccgtttt gcacatagtg ctgagcgtgc tggcgggtta gcgggaaagc ccctgaagcc
    12361 tgcacctgaa tgttgcctaa ttctgcgttg agccggcttg ggaaatgttg ctcaatcgca
    12421 actcccagct tttctgctgg aagcattttc agttcggcaa ttttttgtgg tgaatcgtac
    12481 caaacaacgc agccgtgatt gccggaaagc ggtaaaaagg ctcgagggcc ggttggatag
    12541 aattgttgcc aagtgccgct tggagcaggt ttttcggtgg taacggtggc gagtaaacag
    12601 tgttggcgat attgccaagc ggttaaacca atgcccgccc attggcgaac ctgtgaattt
    12661 gcaccgtctg cggcaatcag cagtggggca gcaaaggtgt tgccatcaac cgtcacttcc
    12721 caatactcgc ccgctttatt tgcaaaaatt tgctcaaatc cgaccgcttg taggcagttt
    12781 ggatattgct ttaattgctc ccataacgca atttgaataa cgttgttttc gaccatatcg
    12841 ccgagctttt ccaaacccaa atcagctgcc gaaaaaagag tgttgaatcc atcaatttcc
    12901 caggtttcga gctgggtata aggggtgctt ctcattgcaa caatttgctc ccacacgccc
    12961 aatttttcta acagttgaat cgagcccata ctaatggcgg aaattcgcaa atcgtagtct
    13021 gaatcaggat taaaggtcgg cagagggttt ttctccaaca aggctaaacg cagcccctgt
    13081 tttgctaagc ctagagctgt tgctgcccca atcataccgc caccgattac aatcatatct
    13141 aattcagttt tcattttttc ttccttttta gttaggcaaa atactgctaa aacgccttga
    13201 attgtagtgc atttcagatt aggatcaaac cgatttatct atttatctaa ggagcgtttt
    13261 atgtcttgca aaactcatgc aaaccacgag cacgaacacg gcgaaggctg tggtcatact
    13321 gctattaaac ataacgacca tatcgactat ttacacgatg ggcatttaca ccacaacaaa
    13381 cacggcgatc actatgacga acacgtttta gaagtgaatg aaaccaaccc ggcagactgt
    13441 aatgccgccc attgctgcgg cgaccacgtt catggtgagg tttgcggaca cgaagctgtg
    13501 ccgcacggcg atcatatcga ctacatcgtg aatgggcgtt tgcaccacca acacggcgac
    13561 cactgcgatg accacgggcc ggttgaaatt gtgaaataag atttttgatg ggaggtttta
    13621 gcctcccatt attatttttg cgatctcgct cgaaaaattg aacattcctg ctacccctct
    13681 tccgattttc cttttatgct ctcggtattg atttctaata ccgaaatgtg atgaccttag
    13741 cgattgttta cagccgagcc tccattggcg ttgaagcccc gttggtgacg attgaagtgc
    13801 atattagcaa cggacaacca aaattaacca ttgtcggctt gcccgaagcc acagtgaaag
    13861 aggctggtga tcgtgtgcgg agtgcgttgc tgaacgctaa ttttatctat ccgccacaac
    13921 ggatcacgat taatcttgct ccggcagatt tacccaaaga aggcggacgt tttgacttgc
    13981 cgattgcgat tggcattctg gcagcctcag ggcaaattga ttctgattta ttaacgcagt
    14041 tcgagttttt gggcgagttg gcactgacag ggcatttgcg aggagtgcac ggcacgattc
    14101 cggcagtgat ttctgccgat aaagccaaac ggcagatgat cttagccaag caaaatgcga
    14161 atgaggcatc gcttgtttcc aatgccacca cctactttgc cggctcgttg ttggaggtgg
    14221 taaatatgct caacaaacgg gataaactgc cgatttgtca gcacatttcg caacattctg
    14281 ccgaaattcg accgcttgtc agccgagatc ttactgatat tatcgggcaa caacacgcca
    14341 agcgagcctt aatgattgcc gctgccgggc aacataattt gctgtttctc ggaccaccgg
    14401 gaacgggtaa aacaatgttg gcaagccggc tggcggattt actgccggaa atgaccgatg
    14461 aggaggcgat tgaaaccgcc tcggtgacca gccttgtgca aaatgagctg aattttcaga
    14521 actggaaaca acgcccgttt cgttcgcccc atcacagtgc atcaatggtg gcattagtgg
    14581 gaggtggctg tgaaaactag aaataaatgt caaaaacaag aaataaatgt cacaaatctt
    14641 tctctaattt cgctataatt cgatcatcaa aactttaagg ggaaatccgt tatgttctac
    14701 ctcaataacg acaatttcaa acgagaacct tatcgtggat attgtccgag tcaccttgaa
    14761 aagcttttta ctaaaccaca ccttgatatt agaggtaacg gttattatta ccctgacaat
    14821 gaatatgttg tagcttggta tgaatcggac ttcattttca aatcaagaaa agctcaagca
    14881 catgcaaaag taaagcttgt taatttaact tgccccaaat acactggcaa aaacagtgct
    14941 gaatatttaa gcgttttgta tatctccgtt ccccttaatt atctacatta tttcactatc
    15001 ggcagtatct ggaaaggcgg tgtcgccaaa gagcagtttg ctctcgaaga attttacatt
    15061 acggttgaag ctgaaaatca agatggtaaa cgagaaaacc tctcaatcgt ttcattcggt
    15121 gagagtgagg aaaacggttt agagaaacca tttgattaca caatctacac tatccccaca
    15181 gaatatcaca actacggtga tgacctaaac cgactacttt ccatctctta ccaaaatcaa
    15241 aaattcatta tccatccgct acatattttt atgatgcact acgggtactc tacggacatc
    15301 aagcgtatct tggcaacata cccacttgac gaggtaagag aacgcctatt tatagataag
    15361 gtcgttgaga attttgatgt agagcgttat gtcgttttac cgaaatactt tgtaaaaaaa
    15421 gatgcgattt tcctctatca cttcaaatat gacgaagaga ctacgggaac aagggtaaaa
    15481 aagcttgtaa gccagtttag gttaaatatt cacaatcaaa cacatccaat agaaatagat
    15541 ttttggcaca cgcaaaaggt tgaattgaaa atccgtggtg ttcgacttgg aaatgctgtg
    15601 ttatgcgcag aaattatcgg tcttaatcag cccgaaggcg aagatattac gctagtgcta
    15661 tctcaatcga aaagagaaaa atcgggtaat aaaaccgagg aacaaaataa cgaggtaaat
    15721 aacgttccta ttaccagagt ttatacacga gaacctgaat tggatgagtt accactcacc
    15781 gataactccc cagataatcg aacagtcgaa tacaacaagc gacaatttga actcttgggc
    15841 cgtcaacgcc aaatcagagc gctcaagaaa gcacgcgaag agagtaatca gaaaatgaaa
    15901 gccttgactc cagatgagat agactcactg ggtgtaggtg aatcagatgg ccgaaacggt
    15961 aaggttggac tcgctttctg tttccttgat gatacacctg ttggcaaaag ctccaaacta
    16021 tacaaattat ggcagcacgc gaaggctgtt gcggaatgga atcacggtga ggcgcactgg
    16081 tacacgccta atctcggttt tagaaatgac gatgaactcc ttcccgtttc cttagaaacc
    16141 aacaaatgtt tcgactaccc tgaaatcgcg ataattatcc gcctccaaat atacggagaa
    16201 acatttttcc tgattgattt ctcaatgaag caaagagata taagcattcg tggcttaggg
    16261 tataaacccg atccaaacga agattttctc tatgaaagtg atatgagcaa aagcgaatta
    16321 tccgaattat tgtccgcagt tcatcatcat gaacatctgc caaaagagta catagagaaa
    16381 aagaacaaag aaaaaaccaa actcactaca tttaaccata gcgaagccga aagtagtaac
    16441 tgggcttata acgcggtgca aaaattaact tcaagggcaa ttactaagcc gacatatttt
    16501 taattatttt tatgaatgca gaacaacgtg taattgttac tccgacattt tggggtaaat
    16561 tttttggaaa aattaaaagt gttgaacttc aacaaaacaa agttatcgta actgacaaaa
    16621 agaataatat aactgaacat gatttaggaa aaacttttga ctttccagct atccagaagt
    16681 ctttctttgg tactaaatta ttttttaaag acgatagcac agaagttgtt ttatcaaaac
    16741 ttgcgaaaaa acaaactgat agtctattac ttgaaatcga aaaagtagtt gcatctaata
    16801 ttaaagtgaa agtaaaagag ggttttcaac actttgcaaa tttagccgaa aatcaatatc
    16861 tcagagattc cgacattcct acattaaatg atcgtgtaag attgtcagtg ctttcctatg
    16921 gggataacaa agaacatttt caaaaatact ttgatgaaag tttggttaag aaaattcaat
    16981 atatctcttc actttttcct gtaaatgcca taaatgctca aacaattcga agagaatacg
    17041 agcaacatgt tttaaatcat cgcaagcatt tctttgatgt cattgaatct aatccgctta
    17101 ctgagcaaca aagattgtca gtcattcgga acaacgacat caatatggtt cttgctgccg
    17161 cggggacagg aaaaacgtct gtaatggtcg caaaagcatt ggacttaatt gattctggtg
    17221 cttgtaaaag cgaagatatt ttgattcttg catacaaccg cgatgcagca accgaactat
    17281 ccgaacgctt acaagaacgt gcacgagaag ccaatttaga tctggaaaat agccctagca
    17341 tctcgacttt ccatgcgctt ggccgaagaa ttattaaaga atgtggaaaa tcagtccact
    17401 tgtcaaaatt tgccgaagat ccgatgaaat tagatagatg gttcaacaaa tggctagagg
    17461 actacatcaa agaagatcct gataatcttg caaaattcat tcaacttgca taccaaccta
    17521 tcgatccatt ccaattcaaa acgaaagacg aatacgatag atatgtaaat gacaatgact
    17581 atcgaacctt acaaggcgaa cgcgttcgtg gttatcaaga gttattgatc gcgaactggc
    17641 tttttagcca cagcgtagaa tatgagtatg aaccaaggta tgtatcaaaa cgccgtatcg
    17701 agattggttt tgactacaaa cccgatttct ctttgggtga tggtgtttac cttgaacatt
    17761 tcggcattga ccgacaaggt agaactcgcg cagatattaa cgcgcaggaa tataatgcca
    17821 atatccaaag aaaacgtgag ttacacgccg aacataatac aacattactg gagacatacc
    17881 actacaactg ggtggaaaat acgttatata aacgactaga gcaattgatg aatgaacagt
    17941 tcattcctct gaagccaaaa tcacaacaag agattttaga tgcactcaat gaatcaggaa
    18001 tcttcaaaga aaacaaaaat cgctatttga agtgccttca agctattcgt acagagcgat
    18061 tagactatca acaaatctta aaacgtctaa ccgatgcgaa aattgtctat gctaaagaat
    18121 atgcgacatt gctcatgcgt attcatgatg cctacgttaa agaactacgt agcgcaaatg
    18181 aaatcgactt tgacgatatg attttgttgg ctactcaact ggtgaaaaca ggtgagttta
    18241 aacctaaatg gaaacatatt ttagtcgatg aatttcaaga tatttctatg gctcgattgg
    18301 agttattgaa agaaatttat actaaagggc ctcgaccgat ttggactgtt gttggagatg
    18361 actggcaatc aatctatcgt ttctctggcg gtaaactaga agttactacg cgatttaatg
    18421 atatggtcgg ctcacacacg ctaagtaaac tagaaaaaac ctaccgctat aacaatagta
    18481 ttgctgacac agcaggtcaa ttcattatgc aaaatcctga gcagtatcag aaaaatgttg
    18541 taacgcatac aaaggtcgct gattcttgtg ttcatttgta tgactctcat gtcgtaaagg
    18601 atgaaaaatc agaagccaat atcagtttaa aagcctcagc aatattaaaa ctgatacgtc
    18661 aaaaagcacc tgaagccact gtcgcaattt tggctagata ccgttacctt ttagaagatg
    18721 caaaaagtgt aatcaaagat aagaatgtta aattttggac gttccacggc tcaaaagggt
    18781 tggaagcaga ttactgcatt ttacttggtt tcttccaagg aaaagtcgga tttcccaacc
    18841 aaaataaaga agaagccgtt gtagaagcat tgcttcctat gctagacagt tttccacact
    18901 cagaagaacg taggttattt tatgtggcat taacgcgtgc gaaaaaagaa agttatttga
    18961 tcgcagatgc gaacgcgcca tcagaattta tcaatgaatt attatcaccg aaatacaaga
    19021 tagatattgt gtcaaaacga ttccaagaac gttatcggac aatctttaaa tgtaatgaat
    19081 gtagcactgg ctatttcgtg atgaaacaag gtaaatttgg tcagttctat tcttgtacat
    19141 caggggaagt atgccgctct tcgccacgga tttgtgaaaa atgtggccgt cctatgaagc
    19201 tgcgagaagg taaatttggt cagttttggg gatgctcagg atatggaatc aaaggcgatc
    19261 aatgtacgaa tactcgtaag tattttatgt aatatattat tttggaggat tttgtggatt
    19321 tatttgaatc aaaaaaacaa ttaatagagt taattaaaaa gtacgatagt gataaagaaa
    19381 tttattcttc atcctcatac aatgagtctc agttaagaac tgacttttta gaccctttct
    19441 ttggtttact tggatgggat ataactaact caaagaataa actcacatct gaaagagaag
    19501 tgattgttga agaaccacta aaagaaaaag tttcagaaaa tgtaaaaaaa ccagattata
    19561 catttagatt caattctcaa agaaagtttt ttgttgaagc taaaaagcca agtgtcaaaa
    19621 tcaagactga ccctttacct gctcatcagg taagacgata t
//
LOCUS       DANLUA010000022        19380 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLUA010000022 DANLUA010000000
VERSION     DANLUA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19380
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2593
                     /gene="adhE"
                     /locus_tag="PXB44_000626"
                     /gene_synonym="adhC"
     CDS             <1..2593
                     /gene="adhE"
                     /locus_tag="PXB44_000626"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL5281137.1"
                     /translation="PFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKASVAALDK
                     HGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGITEIADP
                     VGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRDAAVAAG
                     APENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVGAGNVPA
                     YVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGVYLVNKK
                     EKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNILLAECAF
                     VGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKALAKTFG
                     ERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLINIKRVG
                     RRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRITEQLRQR
                     RNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMWLFYEQP
                     EVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDGDIKYPL
                     ADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGLALQAIK
                     LVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFHTIHGRT
                     NAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAVEAYAQA
                     VHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIEDMEAIMT
                     KAYYGKE"
     gene            2740..3636
                     /locus_tag="PXB44_000627"
     CDS             2740..3636
                     /locus_tag="PXB44_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL5281138.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3646..5055
                     /locus_tag="PXB44_000628"
     CDS             3646..5055
                     /locus_tag="PXB44_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL5281139.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5162..6385
                     /locus_tag="PXB44_000629"
     CDS             5162..6385
                     /locus_tag="PXB44_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL5281140.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6423..7619)
                     /locus_tag="PXB44_000630"
     CDS             complement(6423..7619)
                     /locus_tag="PXB44_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL5281141.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7639..8130)
                     /gene="tsaE"
                     /locus_tag="PXB44_000631"
     CDS             complement(7639..8130)
                     /gene="tsaE"
                     /locus_tag="PXB44_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL5281142.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8130..8684)
                     /gene="orn"
                     /locus_tag="PXB44_000632"
     CDS             complement(8130..8684)
                     /gene="orn"
                     /locus_tag="PXB44_000632"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL5281143.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8694..9173)
                     /gene="coaD"
                     /locus_tag="PXB44_000633"
     CDS             complement(8694..9173)
                     /gene="coaD"
                     /locus_tag="PXB44_000633"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL5281144.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9175..10455)
                     /gene="waaA"
                     /locus_tag="PXB44_000634"
     CDS             complement(9175..10455)
                     /gene="waaA"
                     /locus_tag="PXB44_000634"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL5281145.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10635..12014)
                     /gene="yegQ"
                     /locus_tag="PXB44_000635"
     CDS             complement(10635..12014)
                     /gene="yegQ"
                     /locus_tag="PXB44_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL5281146.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12142..12513
                     /locus_tag="PXB44_000636"
     CDS             12142..12513
                     /locus_tag="PXB44_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281147.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12552..16028)
                     /gene="dnaE"
                     /locus_tag="PXB44_000637"
     CDS             complement(12552..16028)
                     /gene="dnaE"
                     /locus_tag="PXB44_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL5281148.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16253..17350)
                     /gene="lptG"
                     /locus_tag="PXB44_000638"
     CDS             complement(16253..17350)
                     /gene="lptG"
                     /locus_tag="PXB44_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL5281149.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17350..18459)
                     /gene="lptF"
                     /locus_tag="PXB44_000639"
     CDS             complement(17350..18459)
                     /gene="lptF"
                     /locus_tag="PXB44_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL5281150.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18633..>19380)
                     /locus_tag="PXB44_000640"
     CDS             complement(18633..>19380)
                     /locus_tag="PXB44_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281151.1"
                     /translation="QNTIFANDDAQISRYEQAKLRLSYDGNVGNLLQQLSQRLKVGFI
                     AYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKIGERLFLVVSAKDVAPLL
                     QNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIATNQEKIMAAKGKKAPQY
                     KTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFEDIAQGENIIAILHQNTEA
                     PPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         5520 a         4412 c         3783 g         5665 t
ORIGIN      
        1 accatttgat gcccaagcag aagtgaatga gcttgttgaa aaaggcttaa aagcccttga
       61 tgagtttaga aaactcaacc aagaacaagt ggattttatt gtcgctaaag cctctgttgc
      121 tgcccttgat aaacacggta ttttagcaat gcacgcttac gaagaaaccg gacggggtgt
      181 gtttgaagac aaagccacta aaaacctgtt cgcctgtgag tatgttgtca ataatatgcg
      241 acatttgaaa accgcagggg tcattagtga agatgatgtc accggtatta ccgaaattgc
      301 cgaccctgtc ggtgttgtct gcggtatcac cccaaccacc aacccaacct caaccaccat
      361 tttcaaagcg ttaatcgccc ttaaaacccg taaccctatc gtttttgctt tccacccgtc
      421 tgctcaacaa tcttctgccc acgctgctca aattgtgcgt gatgctgccg tggctgccgg
      481 tgcaccggaa aactgtattc aatggattac ccagccttca atggaaggta catctgcttt
      541 aatgaaacac ccgggcattg caactatttt agccaccggc ggtaatgcaa tggttgaagc
      601 cgcttactct tgcggcaaac cggcattagg agttggtgca ggcaacgtac cggcttatgt
      661 agaaaaaacc gctaaattaa aacaagcggt gtatgacatt gtaatgtcaa aatctttcga
      721 caacggtatg atttgtgcct ctgaacaagc ggcgattgta gataaagaaa tttacgctga
      781 gtttattaaa gagatgcaat cttacggcgt ctatttagtg aacaaaaaag agaaagcctt
      841 attggaaaaa tacattttcg gtattgataa agccaaagat gaaaactgct caggtgcaaa
      901 actcaattca gcggtggttg gcaaaccggc tgcgtggatt gcagaacaag ccggctttaa
      961 agtaccggaa aaaaccaata ttctgttagc cgaatgtgcc tttgtgggcg aagctgagcc
     1021 acttacccgt gaaaaacttt cgcctgtact ggctctgtta aaatccgact ccaccgaaca
     1081 cggtttggaa ttgtctgaag cgatggtgaa tttccacggt ttagggcata gtgccgccat
     1141 tcataccgaa aacaaagcct tagccaaaac ctttggcgaa cgggtaaaag ccattcgggt
     1201 gatttggaac tcaccatcaa cctttggcgg tatcggtgat gtatataact ccttcctgcc
     1261 atcgcttacg ctgggctgtg gttcctacgg taaaaactct gtaggtaaca acgttagtgc
     1321 ggtgaattta atcaatatca aacgtgtggg cagacggaga aataatatgc aatggtttaa
     1381 agtgccatca aaaatctact ttgagcgtga ctcaattcaa tatttgaaat caatgcacga
     1441 tgtgcataaa gtaatgatcg taaccgaccg ctcaatggtt gatctcggct ttgtggatag
     1501 aattaccgaa caacttcgcc aacgtcgcaa ccaagtgatg attcagttct ttaccgatgt
     1561 tgaaccaaac ccaagcctgc aaaccgtaca acgtggtaca gaattaatgc gtagcttcca
     1621 gcctgatact atcatcgcac ttggtggtgg ttcaccaatg gatgcagcca aaattatgtg
     1681 gttattctac gaacaaccgg aagtcgattt ccgtgattta gtgcaaaaat ttatggatat
     1741 ccgcaaacgt gcattcaaat tcccacaatt aggcagaaaa gcgaaatttg tcggcattcc
     1801 aaccacctcc ggaacaggct ctgaagtcac accgtttgca gtaattaccg atggcgatat
     1861 taaatatccg ctcgctgact actcgcttac cccaaccatt gcgattgtcg atcctgcgtt
     1921 agtaatgtct gtgccgtctc acgttgctgc cgacaccggt ttagacgtat taactcacgc
     1981 taccgaggct tacacatcga ttttagccaa cgactatacc gacggcttag ccttgcaagc
     2041 cattaagtta gtgtttgaaa acttggaaaa atcggtcaaa gagtttgatg aaaccgcccg
     2101 tgaaaaaatg cacaatgctt ccacaatggc agggatggca tttgccaatg ccttcttagg
     2161 tatttgccac tcaatggcac acaaaatcgg gggtaaattc cacaccatcc acggtcgaac
     2221 caatgcgatt ttactgccac acgttattcg ctataacggc acacgcccaa ccaaagtggc
     2281 aacttggcca aaatacacca attatgtcgc agatgtgcgt ttccaagata ttgccagaat
     2341 gcttggctta ccggcagaaa ccccggaaca agcggtggaa gcttacgccc aagcggtaca
     2401 cgatttagcc gttcgttgcg gcgtgaaaat gtcgcttcgt gagcaaggcg ttgatgagca
     2461 agccttcttg aatgcccgcc gtgaacttgc cctacacgca tttgaagacc aatgcacacc
     2521 agcaaaccca cgtttggcaa tgattgaaga tatggaagcg attatgacta aagcctacta
     2581 tggcaaggaa taaagcacaa tttaatcgtt aaacttacta aatggccgct tgcaagcggt
     2641 catttttctt gaaattttta cttttccctt cacaaaatac tttcgctctt ttacaaagca
     2701 tagtattttt atcgctcctt ttatcttaca ggaaatacta tgcaaattac gattttagac
     2761 ggcggaatta gccgtgaatt aatgcgtcgc aatgctccat ttcatcagcc tgaatggtct
     2821 gctgccgctt tgtatgaagg cccacatttt gtgcaagcgg tacacgaaga ttttatcgct
     2881 cacggggcag aagtgattac taccgacagc tatgcggttg tgccgtttca cattggcgaa
     2941 aaacgctttg cggcagacgg caaaatgctt gccgatttag ccggtcgcct tgccaaacaa
     3001 gcggtcaaaa attcaggaaa tattgcaact caaattgcag gctcacttcc accaatgttt
     3061 ggctcttacc gtgccgattt aattcaaatg gaacgctttg ctgaagtcgc tcagccttta
     3121 attgacgggc tttcgcctta tgtggatatt tggctttgcg aaacccaaag tgcgattatt
     3181 gagccgattt ccatcaaagc attattgccg aaagacaacc gcccgctttg ggtctctttt
     3241 accctcaccg atgatgaacc aaccaccgag ccacaacttc gctcgggcga aagcgttaaa
     3301 caagcagtcg aagaaatggt gaagttaggc gtgcaagcga ttttatttaa ctgctgtcag
     3361 cctgaagtga tcggcgaggc attaagtgta acccaaaaaa ctttagccga attaaaagct
     3421 acccatattc gcacaggagc ttatgccaac gcctttgctc cacaaccaaa agatgccacc
     3481 gccaatgacg gtttagatga agttcgccaa gatttaaccc tggaggctta tttaggctgg
     3541 gcaaaaaaat ggacggaaca aggggcaact attatcggtg gctgctgcgg cattggggtt
     3601 gagtatattg aaacgctggc aaaaaattta aaataaggaa atcgaatgtc aaataaaaaa
     3661 ataggattaa tttcactcac cgccttagtg ctaagctcaa tgattggctc ggggattttc
     3721 agcttaccgc aaaatatggc agccgttgcc ggagcagaag ccattaccat tggctggctg
     3781 attaccggtg tcggcattat ctttttaggt ttatcgtttt tctttatttc ccgcttgcgt
     3841 ccggatttag acggtggcat ttacacctac gcacgggagg gttttggcga gctgatgggc
     3901 tttatgtcag cgtggggcta ttggctctgt gccaccattg ggattgtagg atacctcacc
     3961 gttgcttttg aaggtttggg tgtatttacc gacaacccga acaccgtgat tttcgggcaa
     4021 ggtaacaccc tcgcctcttt tattggatct tcaatcattg tgtggctggt tcacgccctc
     4081 atcgcaggcg gtatcaaaga agctgccttt gtaaacttaa ttgccacttt tgtgaaagtc
     4141 gctccgctca ttctgtttat cgggttaggc atttggtatt tcgatgtgga tctgtttaat
     4201 agcgatgtta aagccaccgc acttaataat agtgtaagcg accaagtgaa aagcacaatg
     4261 ttaatcacct tatgggtatt taccggtgtg gaaggggctt ctgtgctgtc ggctcacgcg
     4321 aaaaagcgtt ctgacgtggg actggcaacc gtattaggta ttattattgc acttgctctt
     4381 tatgtggcaa ttaccattct ttcactcgga attctgccac gggaaactat tgccaatatg
     4441 gcaaatccgt caatggggcc gttgctcgat gcaatgatgg gcccaaccgg caaagtgatt
     4501 attactgctt gtttaattgt atcggtgctt gcctcttaca ttagctggac gatgtattcg
     4561 gcagaagtgc cttatcgtgg ctcaaaaaac ggggctttcc cgaaaatttt agacaaagtg
     4621 aataaaaacg gcacgccgat taactcactc tggtttaccg gctttatcgt gcaattttgc
     4681 ttggtgttgg tgtttgtttt tgagcaaagc tataacacct tattgctgat ttccacctcg
     4741 atgattttaa ttccctattt cttaatcggg gcttatttat tcaaattagc gattcaaacc
     4801 aacgccactt ggtacattaa actcaccggc tttatggcag ccctttacgg attatggatt
     4861 gtctatgccg caggactaga atatttgctg ctttcggtgg tgctttatgt acccggaatt
     4921 ttactcttcc tctactccaa ctataaattc aacggcagat taaccctttc aaccgctgaa
     4981 aaggtcatct taatcgtgct actggtaatt tttggctatg ccgtgattga gctgccagca
     5041 ctattagcag cataattccc atcaaaaatg accgcttgca agcggttatt tttctaggat
     5101 cttctgcaaa gattcattag aatagaaaca acgtcaataa taaataaaaa gaggagaaac
     5161 aatgcaaact tatcaacata aagccctttg tcgggtaaga accattaccc tttttctgag
     5221 tttaaccaaa gataaattgc agtgggaaac ggcattaaat gcggcaaaag cagaatttga
     5281 tctattagtt cccgctattc aaacacaagg ctatcacata caatcgattc gaatcgttac
     5341 caacagtttc ggggaatatc ttgatctcac taatattgaa acagcgaaac aggatttagc
     5401 ctatcttaaa cagctattaa cccaacttaa cgatagcggt ttacgcattc gctttgccat
     5461 tggtgaggca aaaacacccc acgaattagc attactacct gagctgattt taaattatgg
     5521 tgatttatct aacgcttgtg tcaatattga acccgatgaa tttgggattt tagacaatga
     5581 aaccatttta caatctgctc aaattatcaa acaaatcgct gaaattaccc caagaggtga
     5641 aggcaatttt aattttacgg tgaattttaa ctgtaaacct tttattcctt attttccggc
     5701 aagctaccat tcaagcaagc tccctaacag ttttgtgata ggcctagaaa caccagatct
     5761 attagtagcg gtgttaaaag agctgaaagc cagcccacat aacgactttt atcgccaatg
     5821 ctatgaaacg atgtctcaag ccttgcaata tcacgttgat gaagttctcg aagcagtaaa
     5881 atcggtacaa cacaaattac aatttgcctt tgctggcatt gatagttctg ctgctccgtc
     5941 aaaaaactgt gcctcaatga cagaagttta tgaattaatg ggcgtaccat tttttggggc
     6001 agccggaaca gtagaagcct cttcacttct gacaaaagtc tttaaatcta tcaaacatgt
     6061 accgcttgtc ggtttttcag gtttaatgtt ggcagtaacc gaagatttag gcttagccgc
     6121 aggcacacac aaagcccaat tcgacattcg tgctttacta acttatagtg ctgtttgtgg
     6181 cattgggtta gataccgtac cgatttccaa tgaagccact gttgaacaaa ttgccgcatt
     6241 aatgcgagac accggcacaa tggcatttag actaaataaa ccgcttacag tacgtttatt
     6301 tccaattccg aataagagtg ctggggaagt gacagaattt gaaagtgatg atttatgtaa
     6361 ctgccgaatt ttagctgtac cttaaaataa aggctgatgg atatttccat cagcctttca
     6421 atttacttca ccttcaattt tttacctacc gtgatctgat aatttttcaa attattcagc
     6481 tcactgattt tttgcggcgt agtaccgtaa gctctagcaa tcgagtaaac cgtttcatct
     6541 tgttggacaa tatggtagcc actgttagac accttaattt ctctttgaga gtctgatttt
     6601 ttagactgat ttttttgatc tttctcaaga gctgtttttt cttttagttg ctgctctttt
     6661 ttcttattgg ttttagccga atcttctttt ttatcctctt ttttgctctt ttctttcaat
     6721 tttgtcttat ctgattgcgt attcttctct tgtttctcag aagcaggctt tttagttaaa
     6781 tcagctttag cagttttttc ctctttctta ttttgcttat cttctttgtc cgatttttta
     6841 ctgaccgaag aactagcaac tgcatttttt gcccgataag cgactaatcc attataaatg
     6901 gcccgagcaa cttgtttacg ataatttgca ctggctagct ttgcttcctc ggttgaatta
     6961 gataaaaatc ccgtttccac caatacagat ggaatatcag gtgaacgcag aacacttaag
     7021 cttgcgtgct gaggagacga ttttgctaat gcagccacat ttgacatttt agctaaaatg
     7081 ctcttgccta attcatagcc ggcacgttga gagtgagaga actgtaaatc aagcactgtt
     7141 tgatttaaat aacgttcatt gttgcttgaa agcaccgaac cagccccgcc aagtaattcc
     7201 gactgcttct cgtggtcttc caaccattta cccatttcat cgctagcacg gctattagaa
     7261 agtacccaaa ccgaagcccc tttcaaggaa tcgccacgag gcgaagagtc tgcgtgaata
     7321 gaaaccagca gatcggcacg atgcttacgg gcaatctcgg tacgttgcgg taattggata
     7381 aaataatctc ccgagcgtgt catcactgct ttaaaattag gatcggcatc taacaaggct
     7441 tttagctctt tagaaattgc gagggtaacg cttttttcca aaatccccag atttttacca
     7501 atagcccccg gatcttttcc cccgtgtccg gcatcaatcg ccacaatggt tttggcaaat
     7561 gtggaaaaac tgaataaact caatgtgcta aaaacaataa atctgataac ttgcttcatt
     7621 acctccccta caagcggtct atttttgatg aaaatttact aatatatcta ctgcagtttg
     7681 attttgcggc aacaggctaa tctgccgtcc gtcattctta taatcaattt ggatcacaaa
     7741 atcagcctct ggaatcattc ctttaccttt aactgcccat tcaagcaaac aaagcgtttg
     7801 tggtttgaaa taatcacgaa ttcccataaa ctccagctct tcaggatcgg ctaagcgata
     7861 caaatcaaaa tggtaaaggc tgaaaggcgg aagttggtat tcttccacca gagtataagt
     7921 tgggcttttt acattgcctt gatgcccgaa agcccgtacg atcgagcggg ttaaggtggt
     7981 tttacctgcc ccaagttcgc cgtttaggta aacaaccagc gaatgagctt ggtcttgcaa
     8041 taaatatttc ttaagttcta acgcgaatga ttgaccaaat tccagcaatt tagtttcatc
     8101 ttcgcagtaa aaagagagag aatctgtcat tatttttggt ctagctgaat aaaatgttcc
     8161 cgataaaatt tcaattcgtt aatggattca cgaatatcgt ccaacgctaa atgcgtattg
     8221 cctttactga atttatctaa aatctccggt ttccaacggc gtgccagctc ttttaaagta
     8281 ctcacatcta agtggcgata atggaaataa tcggctaaat caggcatata tttatacaaa
     8341 aaacgtttat cttgagcaat gctgttgccg caaatcggag aagcgccttt tggcacccag
     8401 cgttttaaga agtctaaagt ttgcaactct gctgcccgtt cagtcaattt gctcgctttc
     8461 actcgttcaa tcagcccgtt agcggtatgg gttttgacgc accaatcact cattttctct
     8521 aataaatcat cggtttggtg taccgcaagt accggtcctt ccgctaaaat atttaaatcc
     8581 ttatcggtca caatggtggc gatttcaata atgcgttctt tttccggatc taaccctgtc
     8641 atttcaagat cgatccaaat taaattttgt tcgttttgag ccattttatt tccttacact
     8701 tttgcaattt ttagggcatt tttgaccgct tgcggcacaa attggctgat gtcaccattg
     8761 tggcgataga tttcccttac catcgttgaa gaaaggtaac gccattctat tgaaggggga
     8821 aaaaaaatag tttctaaatc gcccgataat ttgtaattta actgagcgag ttggatttca
     8881 tagtcaatat catctgcccc acgaatacca cggattaacg ctttggcatt ctgctgtttg
     8941 gcaaagtctg ctaacaatcc actaaagcca atcacctcaa tattttgcca ctgggtacag
     9001 ctttgtttaa caagctccac tcgttcttca agactaaaaa gcggctgctt gcttgggttt
     9061 tgagcaacgg caacaatcac ttttccaaat aagtgagagg ctttgcaaat tatgtccaaa
     9121 tgcccattgg taatgggatc aaaggtgcct gcataaatta cgttataact cattttattt
     9181 ctctaaatag gaggctaata aatccaaatg gcgttttaat gccccttgat tttcctgtaa
     9241 cacgcttaag ccggattttg caatttcttg tccgatttgg ggctgcttaa gcaataattg
     9301 aatgctctcg gtcaaagcct cgactgaact ttcaatttca atcacgcctt tcacctcacg
     9361 caatttctca aagatttcag ggaaattata ggtgtaaagc ccagaaacca ccggaatatt
     9421 aaaagcgatt ggttcaagtg gattatgccc accgtgtttg accaaactgc ctcctacaaa
     9481 agcaatgtca gacaacccat acagtaacat catttccccc attgtatcac ctaacattac
     9541 ttgagtattt tggcttaacg ttttattctc agtacgtttt acataattta aatctgattt
     9601 ttgaatcagt agctcaacta aaccaaaacg ctccggatgg cgaggcacta aaatcaacac
     9661 taaattcggg taatgctcta ataagagttt atgagcatct aaaatcagct tctcttcccc
     9721 ttcgtgagta ctgccggcaa tccaaatcgg tctctctgtt aaattcagac tagctttagt
     9781 tttaatcacc gattgatgaa gctgtggcgt aatttctaaa tcaaatttca ggttaccggt
     9841 attaaccatt tttgccggtg cataaccgag tgctaaatag cgatctcggc ttacctcatc
     9901 ttgagccata atcagactaa tttggttgag catatcctta atgctacctt ttacccagcc
     9961 ataacgcttc gcagaacgtg gcgataagcg ggcattggca atcacaaatg gaattttacg
    10021 aatatttact ttacgaatta aattcggcca aagttcggtt tcaattacaa tcatcaattt
    10081 cggatcgata aaattcaaaa accgctcgat agcatcaggt aagtcataag gcaaataaaa
    10141 atgtgaaaca ctattaccaa aagcagcttg cacacgccca gagcctgttg gcgttaccgt
    10201 tgtgactatc aacggaagtt caggatattg cttgctgact gccttaatca aaagcgttgc
    10261 agcaatcacc tcaccaaccg atgccgcatg gattaccacc ccttttgctt tcggtttttc
    10321 cgattcgtca taaatcccat aacgctccca taaacgtttc cgataagccg gttgtttacg
    10381 ccctttgtac cacattaata gcagaaccac aggctgtaat agataactta aacaaatata
    10441 gagtaaacgt agcatttatt ttccttatac cataaccctc acctcttgtg ggagagagtc
    10501 aaatacgcca ataaatcgtt cctctcttgt tcgagagggg ggaacaagcg gttattttta
    10561 ccaaaaattt tgcaatctat atcctcaaag acaaaaccac ggaataacct taatccgtgg
    10621 tttatctcgc atcattacgc ttgatgtgga ttgcgggtat tcgtattcac taagttacgc
    10681 attaaaagtg caaaatccaa ctcaatatct gccggcacat ctaaaaagac aaaatgcccg
    10741 tcaccgagtg ctgcgtccac attttcattt ttgcggttaa gcatacgctc gattttaaat
    10801 acaatgttgc ctttaggggt catcatttcc actgaatcgc ccactaagaa tttgtttttc
    10861 actgccactt ccgccaagcc ttctgcattg cgtttgccgg taaattcgcc cacaaattgt
    10921 tggcgttctg aaatcgaata gccatattca tagttttggt attcatcgtg agtatgacga
    10981 cgtaagaagc cctctgtata accacggtgt gcaaggcttt caagagtatc cattaaactt
    11041 tcgtcaaacg gtttaccggc agcggcatca tcaatcgcct tacgatacac ctgagccgtt
    11101 ctcgcacagt aatagaacga tttcgtacgc ccctcaattt tcagtgaatg cacccccatt
    11161 tgagttaaac gctcaacgtg ttgtactgca cgcagatctt tagagttcat aaaataggtg
    11221 ccgtgttcat cttcaaaggc ggtcatttgc tcatcagggc gttgtgattc tgtataaagg
    11281 aacactttat ctgttgcttc gccattacct agagtaggag ccacattttt gacttcaatt
    11341 tccggctcat acttaggcac aatttgccct acctcatcaa tcttaccttc ttccatttta
    11401 tattcccaac ggcaagcatt ggtgcaagtg ccttggtttg ggtcacgctt attgatatag
    11461 cctgaaagca agcagcgacc tgaatatgcc atacacaaag caccgtgaac gaagatctca
    11521 agctccatat ccggcacttg ttcacgaatt tcttcgatct cttcgagtga taattcacgg
    11581 gataaaatca cacgggttag ccccatttga cgccagaatt tcaccgttgc ccagtttacc
    11641 gcattggctt gcaccgaaag atgaatatcc atttcaggga aattttcacg caccaacata
    11701 attaagcccg gatcagacat aatcaaagcg tccggcttca tatccactac cacttttaga
    11761 tctttgatga aggtttttaa ttttgagttg tgtggcgcaa tgtttaccac cacatagaat
    11821 ttttttccta aggcgtgggc ttcatcaatc gcaactttta aattggcgtg gttaaattca
    11881 ttattacgca cacgcaagct ataacggggc tgtcctgcat aaatcgcatc ggcaccataa
    11941 gcaaaagcgt aacgcatatt ttttaaagaa cctgcaggcg aaagtagctc aggtttggca
    12001 tattttaaag tcatattatt ctcttttctg ataacaagtc aggacttctc gacgtgagaa
    12061 gcgtaagcgg tatattttct aagaaatatt gcgtgatggc aagctaatcc attacaatcc
    12121 gatttttatg tggggaacaa tatgaaactt aaataccttt tagcaacaac attactggct
    12181 ttttcaacat ttaccgccgc tcaaagcatc aagacggaga tgatgcaaat gaatatgcaa
    12241 gcgaataaat tgatgcgtgc cgaatcggca gaagagttta gcgaaattgc gaatgatttc
    12301 attataatta ccgagaaaac caaagggatt tttcccaata gcgtagaagg cgataaagca
    12361 gctcaggaag attaccaagc cggtatgcaa aaattaattg atatggttaa tcaagccagt
    12421 gagaaagctc aagcaggtga attgcaagaa gcaaaaatgc tgatttccga gcttgaaatg
    12481 atcaagcgtg aatatcacaa aaaatacaaa taattcaaca cattaaaagg gtgagctaaa
    12541 gctcaccctt tctattcaaa ttccagttca accgaattct caccaagaag tgcctttaat
    12601 tcgctaaaca ttgcctcatt cgggctgatt cgccactcta ctccgccttt gagcaatacc
    12661 ctaccttccg ggctttggta ataaaaatgc agcggtaaag tgccttcttt atgtggggtg
    12721 atgatagcgg tcattttttt aataaaatct gcagaaagtt gctctttgct aatcgctaac
    12781 gctaaacttt tggcaaatcg accccttgct tcatcaaggg ataacacttc tcgagccgac
    12841 attttcagtc caccgctgaa atcatcaaac tgtaccgaac ctgttgccac aataatttgg
    12901 tcttgttgca tcaaatgccc gaaattttct aaggcttccg agaaaagggt aatgtccaat
    12961 ttccccgagc gatcttcaat agtcgcaatc ccaatacggc ttcctcgctt ggtaactgcc
    13021 actcgagatg ccataacaat tcctgaaacc gtggtcattt gccctcggaa agtcggttgc
    13081 aattcattca gccgattagg ggcatagtgg gataattctt tgaggaatct accaatcggg
    13141 tgtccgctta aatacagccc gagcgtggca cgctctccat ctaaaatcac ttgctccgac
    13201 cattttggcg tattggcgta agcattttgc acttcttccg gtgtctcggt aagcacgcca
    13261 aacatatcgc tctgcccgag ttcctccatt ttagcgtgct gatctgaggc tttaagtgca
    13321 tcttccaaat tcttcatcaa ggcagctcgg tgagggccta atttgtcaaa agcaccggac
    13381 ataatcaggc tttcaaaggt gcggcggttg atttttttca gatcgacccg agcggttaaa
    13441 tcaaatagat ctttaaattt cccctctttt tggcgagctg ccagaattgc ctcaatcggt
    13501 ccttccccaa cgcctttaat tgctcctagc ccgtaaacaa tttcgccttt ttcatttacg
    13561 ctaaaacgat gtttaccgct gtttacatca ggcggcacaa cggttaagcc catattcata
    13621 cactcatcgt agaacccgac aattttatcg gtattatcca tctctgaggt cattaccgca
    13681 gccataaatt ctgccggata atgggctttt agccaaagtg tttggtaaga aaccaacgca
    13741 taagctgctg agtgagattt attaaagccg tagccggcaa acttttctac caagtcaaaa
    13801 atttgaccgg caagtttacc gtcaatccct ttggcttccg cccctttttc aaacacttca
    13861 cgctgtgcag ccatttcctc cggtttcttc ttacccattg cacggcggag taagtccgct
    13921 ccaccgagtg tataaccggc aagctcttgt gcaatttgca taacctgttc ttggtaaaca
    13981 atgaccccat aagtcggctc gagaataggt tttaagcatt cgtgttggta ttgcggatca
    14041 ggataagaaa cctcttcttt gccgtgttta cggtcaataa agttctgcac catacctgat
    14101 tccagcggcc ctgggcggaa tagtgccacc aatgcgataa tatcttcaaa acagtccggc
    14161 ttcaggcgag aaattaagtc tttcattccc cgtgattcaa gctggaatac tgctgtagtt
    14221 ttagctgcca gcaacagagc aaaggatttt ttatcatcaa gcggaatggt ttcaatccga
    14281 atcggctctt tgccttcacg agccaaacgt tcgttaatca tctccaacgc ccatttgatg
    14341 atggtgagcg tacgcaagcc taagaagtca aacttcacca atcctgcata ttccacgtca
    14401 tttttatcaa aatgggtaac cgggtgtaag ccctcgctat cgcaataaag cggcgaaaaa
    14461 tcggtaatcg cagttggggc gatcaccaca ccaccagcgt gtttacctgc attacgagtt
    14521 acaccttcga gcttacgagc catatcaata aggtctttca cctcttcatc ggcttcatat
    14581 agctcaggca attttggctc agcggcaaag gctttttcaa gcgtcattcc tggatcgggc
    14641 ggaatgagtt tggagatgcg atccacaaag ctataggggt gtccaagcac tcgccctaca
    14701 tctcgaatga ccgctttcgc cgccatagta ccgaatgtga taatttgcga taccgcttgt
    14761 cgcccgtagg tttccgcaac gtgttcaatc acacggtctc gcccgtccat acagaaatca
    14821 acgtcaaaat cgggcattga aacacgctca gggttgagaa aacgctcaaa aagcaaatca
    14881 aattccaatg ggtcaagatc ggtaatcttc aacgcataag ccaccaatga gcctgcccct
    14941 gagccacgac ccggtcctac cggaatatca ttatctttgg accattggat aaactccatc
    15001 acgatcaaaa agtaacccgg gaaccccatt tggttgatca catctaattc aacttgcaat
    15061 ctttcgtcat aaaccgaccg cttgttcgcc ctttcctcag gatcagggaa tagaaactct
    15121 aaacgctctt ctaagccttc tttcgatttt tttaccaaga aatcttcggt ggaaagctca
    15181 ccggtcggga aatttggcag gaaatactcg cccaaacgca cggtaaccga acagcgtttc
    15241 gcaatttcaa ccgtgtttgc caatgcactc ggtaaatcgg caaacagctt gcacatttcc
    15301 tcttcagtac ggaaatattg tttcggtgag tattttttcg ggcgtttcgg gtcgtctagg
    15361 gtgtagctgt cgtgaatggc aacccgaatt tcgtgtgcgt caaagtcatc ttctttcaag
    15421 aagacaacat catttaccgc cactaacgga attgcatttt tttgtgaaaa ttcgaccgct
    15481 tgtttgatat agcgttcttc gtctgcccgt ccggtacggc aaacggaaag gtaaaaatga
    15541 ttcgggtaat attgctgata aaaagctaat ttttcttcag cttcttgtcg gttttctttg
    15601 agtaaggctt tacccacatc gcccattcgc ccgccggaaa gaatgatgat gccctcattc
    15661 agctctgcta accattcacg ttccaccatc ggaaattcca catagccttg ctgataggct
    15721 ttggacaaaa tttgggtaat attatggtag cctttgttgt tcttcgccag taatgtcagc
    15781 tcaaaataat cttcattgcc gctctcggtt ctaacataaa catctgcccc gataatcggc
    15841 ttaatccctg ctcccaatgc ttcgccatag aatttcacca acccgcaaaa gtttataaaa
    15901 tccgttaagc ccattgccac catattatta gcggcagcgg ttttaactaa ggattttacc
    15961 ttagctaaac cattaatcat tgagaaatca ctgtgaactt ttaaatgaac gaaacgacaa
    16021 tctgacatag gaatataatc taagtaaaga agtttcccct ctttagtaaa gactgtcagg
    16081 ttctaaaaag tgaagccttc tgtgaataat cccgttgggc gaatgttatt cgcccctaca
    16141 aacgagcatt attcttgctc agagttacat ttaaaatccg aatgtagggg cgaattacat
    16201 tcgccctttc agaacctaac aaccctgctt tatctaaaga agggagttaa aattaatcac
    16261 gttttttgct taacagccac caaacgatga tcaaacagag taggcttgga attaatgctc
    16321 caaccggtat aggtaaccaa gtggcaatta agcttaaatt gccaaacaca atatttgcca
    16381 catagaacat aaagccggca acaataccaa tcacaatttt cgcccccatc gtactactac
    16441 gaagcggccc gaaaataaag gagatcgcaa gtagcatcat caccgccatt gagattggtt
    16501 ggaatacttt tcgccagaag gtgatctcaa aacgtttcgg atcttgtccg gtatctttta
    16561 agaagccgac ataatctgcc aagcctgaaa tcgagagcga ctccggtttg agtgaaacaa
    16621 tgcctaattt gctaggggtt aaactggttt gccaaacttg atctggctgt ttagcttgct
    16681 ggatctgatt ttctcccaca gtcgattttt ccactttaga tagcacccag cctttttcat
    16741 cactgtaatt cgcagttcgg gcttttaaaa cagattgcaa ttcacgtttg ttttgtcctt
    16801 cgccgaattg ataaatcagt acgttatcta agcttttttc atttttaata tgattaatat
    16861 agataaatga attgccatct ttcgcccaaa acccgtcttt tgtggcaagc attgagccgc
    16921 cactttgggc aatcgaacgc atattacggg caaactgctc ggtttgtggc acgccccatt
    16981 cgccgataat catcgtaaaa atgactaacg gtaaggcagt tttcatcacg gctaaaccaa
    17041 tgcggaaacg ggaaaaaccg gaggcttgca tcaccaccaa ttcactaagg ctcgctaggt
    17101 tgcctaaacc gagtaatgag ccaagtaatg ccgcaatcgg gaaaaaggtt tcgatatctc
    17161 gtggaatagt taaaaaagta tagtatgccg cttttaagcc atcgtaagat ccacgtccta
    17221 catcacgaaa ctcttccaca aatttaataa tcgccccaag cccaatcaac ataaacaggg
    17281 ttaaaaaaat cgcggataaa atcgttttgc cgatgtagcg ttctaaaata ttcattgaga
    17341 aaaattgcat tatgccaccg ccttattggt taattttttt gcaccaaatt gatagcgtaa
    17401 cttagacata aatttactat cccacgcatt taaaataaca cctaaaacta aaaaggcaac
    17461 tgataccaac ggcattaaca cttccgcatt caacttgcct gatgcccctg acgattttag
    17521 cgagctttgt agtaagaaat agattaaata aagcaataac gccggcagta gtttagcaaa
    17581 acgcccttga cgagggttta cactactcat tggcaccgct aataatgcca ttaacggtac
    17641 agctaaaatc aaagcaagcc gccaatgaag ctcggctttt gcttccggcg ttttggtttc
    17701 cattaatttc ttgaaatcgg ctcgttgcac taatttctca tcagattcaa cgtcttgata
    17761 gcctaaataa gcagaataat tctcaaaatg tgaaatacgg aaatcggcac tttgtggtgt
    17821 accttcaaaa cgcttgctct ctgccaaggt taagacttga tccccatttg gcaaaccttg
    17881 caacgtgccg gattcagcaa caaccactga aggtttgttt ttcttctgct gttccggctg
    17941 gaaaacgtag atatcattta gactgttatt ttcaatttta tcaataaata acacatagcc
    18001 accggcagac ataaattgtc cggccgaaag agccgcaaaa cgtgggttgg ctttcgcttc
    18061 cgctaacatt tcactctgtt tattaattgc ccaaggcgta agccaaaaca cgttataaac
    18121 cgctaatgca gtggtaaaaa tcgagagaaa catcgccact ttaaccaata gcccttgccc
    18181 gataccacaa gcccgcatta cggtaatttc actttcggca taaaaccgcc cgagggttaa
    18241 taacaaggcg ataaacagtg agagtggcag cattaattgt gccattgtcg gcatacctaa
    18301 gcctaataag ctcaccacta aatctgccgg tactttacca ctcacagcag aacttagaac
    18361 acgcactaat tgctgacaaa aaaagatcag taataagata aacaatatcg ccaattggct
    18421 tttaaatatc tctttggtta aatatcggct taagatcacg ttttttctct ataacattta
    18481 caaatctgaa taccgctaaa cacaagcggt ctatttttgt taattttttg caaagagcgc
    18541 aaaataactt attcgaatcg ctaataatac ctttttcata caaaaaaatc acctatagaa
    18601 aatatcacgc acggcaaaac cgtgcgtaag tattattgtt ttttcgattt tttcgatgat
    18661 gttgctttat gagcttcaag aattaatttc ttaccttggc tatcttcaat ttcaatctta
    18721 ggtggtgctt ccgtattttg atgaaggata gctataatat tttcaccttg tgcaatatct
    18781 tcaaagtttc ctttcacacg taacttagcc gcatcaacac tagtgtcaaa tactaagaat
    18841 gtacctaatg cggtaactct aatattttta agccctatat tgtccttcgt taccgtttta
    18901 tattgaggtg cttttttccc ttttgcagcc ataatcttct cttgattagt cgcaatatta
    18961 agaatatttt gaactttagt cgcttctgct gtagattgtg cctgttctgt taaatttggt
    19021 tctccatcaa aaataacatc cccaacaaat tgcacctctt taggctcatt ttgcaataat
    19081 ggtgcaacgt ctttagctga aacaaccaaa aataaccgtt cacctatttt ttcaaaacgg
    19141 acatcaatat tggttaattg tgattcaatt tgttcattaa ttgtcttgat cgcagtttct
    19201 gtctcatttt gaagcgatat tttacgtgta atatcaacat cataagcaat aaaaccaact
    19261 tttaaacgtt gtgataactg ctgaagtaaa ttgcccacat ttccatcata agaaagacgt
    19321 aactttgcct gctcatagcg actaatttga gcatcatcat tagcgaaaat agtgttttga
//
LOCUS       DANLUA010000023        19045 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLUA010000023 DANLUA010000000
VERSION     DANLUA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19045
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            110..793
                     /locus_tag="PXB44_000641"
     CDS             110..793
                     /locus_tag="PXB44_000641"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL5281152.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(930..1487)
                     /gene="frr"
                     /locus_tag="PXB44_000642"
     CDS             complement(930..1487)
                     /gene="frr"
                     /locus_tag="PXB44_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL5281153.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(1539..2255)
                     /gene="pyrH"
                     /locus_tag="PXB44_000643"
     CDS             complement(1539..2255)
                     /gene="pyrH"
                     /locus_tag="PXB44_000643"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL5281154.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            2347..3909
                     /locus_tag="PXB44_000644"
     CDS             2347..3909
                     /locus_tag="PXB44_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5281155.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(3980..4825)
                     /gene="tsf"
                     /locus_tag="PXB44_000645"
     CDS             complement(3980..4825)
                     /gene="tsf"
                     /locus_tag="PXB44_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL5281156.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGG"
     gene            complement(4921..5640)
                     /gene="rpsB"
                     /locus_tag="PXB44_000646"
     CDS             complement(4921..5640)
                     /gene="rpsB"
                     /locus_tag="PXB44_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL5281157.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            5985..7013
                     /locus_tag="PXB44_000647"
     CDS             5985..7013
                     /locus_tag="PXB44_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5281158.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            7136..8782
                     /locus_tag="PXB44_000648"
     CDS             7136..8782
                     /locus_tag="PXB44_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5281159.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            8792..9802
                     /locus_tag="PXB44_000649"
     CDS             8792..9802
                     /locus_tag="PXB44_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5281160.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            9812..10438
                     /locus_tag="PXB44_000650"
     CDS             9812..10438
                     /locus_tag="PXB44_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL5281161.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(10500..11990)
                     /locus_tag="PXB44_000651"
     CDS             complement(10500..11990)
                     /locus_tag="PXB44_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL5281162.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(12049..13284)
                     /gene="rhlB"
                     /locus_tag="PXB44_000652"
     CDS             complement(12049..13284)
                     /gene="rhlB"
                     /locus_tag="PXB44_000652"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL5281163.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(13372..14094)
                     /gene="recO"
                     /locus_tag="PXB44_000653"
     CDS             complement(13372..14094)
                     /gene="recO"
                     /locus_tag="PXB44_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL5281164.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
     gene            complement(14324..15241)
                     /gene="era"
                     /locus_tag="PXB44_000654"
     CDS             complement(14324..15241)
                     /gene="era"
                     /locus_tag="PXB44_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL5281165.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(15344..16018)
                     /gene="rnc"
                     /locus_tag="PXB44_000655"
     CDS             complement(15344..16018)
                     /gene="rnc"
                     /locus_tag="PXB44_000655"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL5281166.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(16064..17023)
                     /gene="lepB"
                     /locus_tag="PXB44_000656"
     CDS             complement(16064..17023)
                     /gene="lepB"
                     /locus_tag="PXB44_000656"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL5281167.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(17110..18903)
                     /gene="lepA"
                     /locus_tag="PXB44_000657"
     CDS             complement(17110..18903)
                     /gene="lepA"
                     /locus_tag="PXB44_000657"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL5281168.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
BASE COUNT         5554 a         4065 c         3505 g         5921 t
ORIGIN      
        1 tcagcttttt agtgaaagaa tttaactatc tatttcaaaa tataaagaat tttgctataa
       61 atagcacaat ttcaaatcca ttaattttac tcaaagctaa ggagtcatta tgcaattagt
      121 atttatccgt cacggtttca gtgaatggaa tgctaaaaac ttatttaccg gttggcgtga
      181 tgttaattta acagaacgtg ggattgaaga agcaaaatct gccggtcaaa aattaaaagc
      241 agccggtttt gaatttgata ttgcgtttac ttcggtttta acccgtgcga tcaaaacttg
      301 taacatcgta ttagaagaat cagaccaatt atggatccct caagtgaaaa actggcgttt
      361 aaacgagcgt cactacggtg cattacaagg tttagacaaa aaagaaactg ctgaaaaata
      421 tggtgatgaa caggttcaca tctggcgtcg ttcttatgac atctctccac cggatttaga
      481 cccacaggat ccaaactcag cacataacga ccgccgttat gctaacttac caaaagattt
      541 aattccaaat gcagaaaacc tcaaaatcac ccttgagcgt gtgttaccat tctgggaaga
      601 tcatattgca cctgcattaa tttcaggtaa acgcgtatta gttactgctc acggtaactc
      661 attacgtgca ttagcaaaac acatcgaagg catttctgat gcagacatta tggacttaga
      721 aatcccaacc ggtcagccat tagtgtatga actagatgac aacttaaaag taatcagcaa
      781 acgttactta taattggtaa atttccgata aaaaataacc gcttgtagtg caacacatta
      841 caagcggtta ttttttgtta attttttgca aatggcaaag aacaaaaata aaggcgaatc
      901 atacattcgc cttattgttt taaagtagat tagaaatcta acaactcttt ctcttttgct
      961 gctaatactt catcaacttt tttaatgaag gtatcggtga ttttttgaat atcttcttca
     1021 gctttgtgtt gttcgttttc gctgatttct ttatctttta ataacgcttt gatttggtca
     1081 ttagcatcac gacgaacatt acgaattgcc actttgcctt gctcaccttc tgctttaacg
     1141 attttgatta aatcacgacg acgttcttcc gttaatggtg gaagcggaac acgaatggtt
     1201 gtgccggcag aagatgggtt taaacctaaa tctgaggtta aaattgcttt ttctaccgca
     1261 gagattaaag atttatcaaa tacgtttacc gctaaagtac gggcatcttc agctaccacg
     1321 ttcgctactt gacgtaatgg agtcgcagag ccgtaatatt ccacttgaat accgtctaat
     1381 aaagaaggtt gggcacgacc tgtacgaatt ttcgcaatat gcccttttaa agcctcaagg
     1441 cttttttcca tacgatcttg ggtatctttc ttgatttcgt taatcattga ttttttatcc
     1501 ttttatttag taactgagag ttaaacagag aaaattaatt attcagaaat ggttgtacct
     1561 tcttcagtac cgaataccac attgcgtaac gcccccggtt tgcccatatt aaagacacga
     1621 attggcattc cgtggtcacg agcaagcgtg aatgctgcta agtccattac ttgtaattct
     1681 tgctcaataa cttgcgcata cgttaattta ttatatagtt ttgcatctgc gtattttgct
     1741 gggtctttat cataaacgcc atcaacttta gttgctttta ataccacatc agcttcaatt
     1801 tcaataccac gcaagcaagc agctgaatcg gtggtaaaga acgggctacc tgtaccggca
     1861 gagaaaatca ccacacgttt ttcacgcaac attttaattg cttcagacca gttgtaagtg
     1921 tcgcaaatcc catttaattg gaacgcagac attaatttag catttacctc tgcacggtgt
     1981 aaggcatcac gcatagccaa accgttcatt acggtggcaa gcatacccat atggtcgccc
     2041 actacacggt tcattcctgc tttggctaat tttgcaccac ggaataagtt accgccgcca
     2101 agtaccacac caacctcaac gccttcagca agtaattctt taatctctaa cgccatacga
     2161 tccaaaatag acgggtcgat accaaaacct tcactgcctt gtagggcttc accacttaat
     2221 ttgagaagaa ttcgcttata aataggtttg ctcattttaa ttgcctctta actgtatgga
     2281 ctaaacacaa aattacgtta ttatagaaca atccaatata acgataaact aaaaatattc
     2341 actacaatga aatccaaaca acttttcggc attcttgtcg ctatttcgct ggcaactgct
     2401 gctagctgtt taatgctaat cggatcaggc ttttttccaa atccgagtat ctttgaagtc
     2461 attttgggcg tattcctcat catcgctctt gccagctcta aatggactta ttatttactc
     2521 ttactgccaa tcagcatcgc ttatgcaatt tataccccgg ttgggataaa attcggtaaa
     2581 ccgacttacg agtatatcgc ctctattttt gctaccgata tgatggaaag caaagagttt
     2641 attgctcaac tgccgatttt aaatattgcg gcgggcattg cgattgtact agcggtcatt
     2701 ttttatcgaa aaattaccaa tcgctatcaa atccgttttt tacgcaataa aacattcgtg
     2761 atttcggctt ttgtattaat gctgttttcc cttgcaccgt ttaaattttt tcaggagttc
     2821 tacaattcga gcctaaaggt aaagaaagaa ttggaagtgc tgaacaattt aacgctagat
     2881 tcacaatggg gacaatcaac cctcagctca acttcaaact acgatgatta tgtactgatt
     2941 atgggcgaaa gtgcgagaaa ggattatcac cacgcctatg gctatccggc tgccaatacg
     3001 ccttttatga gttcggcaaa aggaaccttg attgacggtt taacctccgg tggcacaaac
     3061 actatcgcct cattacgctt aatgctcact aagcccgata cggaaaaatg ggaagcaaat
     3121 tatgggttaa ccttaattga tttaatcaaa tctaccggta tcaagaccta ttggatctca
     3181 aatcaaggct atttgggcaa ttacgatacg ccgatttcct ctatcggtaa taaaagtgat
     3241 gtcaaaattt tcactaaagc cggcgattca ttaaatacca atgtgagcga ttttgaattg
     3301 ttaccgcatt ttgctaaagt gattgaagaa cctgctcaag ggaagcgttt catctttctg
     3361 catttatatg gctcgcatcc tattacctgc gatcgtctca ccgattaccc gaaaatgttt
     3421 gacgatgaca aactgccaaa acgctacttt aatgtaaatt gctacgtgtc atctatcaag
     3481 aaaaccgatg aattgctgaa acgggtatat gatttgctag tgaaaaacca agaaaatcac
     3541 cagcgttcat tctcaatggt ttatttgtct gatcacggtt tatcgcacga ggtttcaaaa
     3601 gagaacattg ttatccacaa tggaaaggat agaagtaaat tacactacga tattccgctg
     3661 tttaaaattt caagcgatga tacccaacgt aatgagtata aagcctttaa atccggctta
     3721 aacttcacta atggtattgc gaattggatt ggtatcgaaa atccgttatt agataaagaa
     3781 attaatcttt ttaacgaaca aaacgataaa agtgattatg ggctaaagaa aatcattgaa
     3841 acgcagtcta ctgtagaaga tccagctgtt cctattcctg aaagctactt atataaagcg
     3901 actaattagc aaaagtaagt aacaaaaaag caggctttaa gcctgctttt ttagttttta
     3961 gtatcagaaa aattaagcgt tacccgccag tgattttcgc tacttctgct gcgaaatctt
     4021 cttctacttt ctcaatacct tcacctactt ctaaacgaat aaagttagaa actgaagtat
     4081 ttactgattt taaatattga cctacagatt gagatggatc cattacaaac ggctgaccgg
     4141 ttaatgaaac ttcaccggtg aatttagcca tacggccttc aaccattttt tctgcgattt
     4201 cttttggttt accagaattc atcgcaattt caatttgaat ttcacgttct tttgctacta
     4261 catcagcaga aacatcttct gggtttacaa attcaggttt gcttgcagca acgtgcattg
     4321 ctactttctt aagctcatct tcagagcctg cacctgctac taatacacca attttcgcac
     4381 cgtgtaagta ttgagcaata acttcgcctt ctaagtattg aacacggcga atgttcatat
     4441 tctcaccgat tttcgcaact aatgccgcac gtttttcttc aaattgtgct tgtaatgctt
     4501 cgatagttgt acctttattt gcaactgcaa aatcagcaac ttcgttcgct aattctaaga
     4561 aaccggcatc ttttgcaacg aagtcagttt cacagttcat ttcaactaat acgccaaaac
     4621 ctgaagcgac acgagcaagg attacacctt cagctgcaac acgacctgct tttttagccg
     4681 ctttcgcttg accggattta cgcatattat cgattgctaa ttcaatatca ccgttcgctt
     4741 caactaatgc ttttttacat tccatcatac ctgcgccggt acgttcacga agttctttaa
     4801 ctaatgatgc tgtgatttca gccattttaa attcccctta tttgatatgg taaaacaggc
     4861 tctcacctgc ctagaaattt cattgtttag ggcaggttaa aacctgcccc ctcaactgaa
     4921 ttattccgca gaagcttctt ctgctacttc aacagtttca acaccgcgac cttctttgat
     4981 tgcagcaact gctgaatcta agtaaagttg gatagcacgt gttgcatcat cgttaccggg
     5041 aatgatgtag ttaacgccat ctggagttga gttagtatca acaatcgcaa ataccggaat
     5101 acctaagttg tttgcttctt taattgcgat atgttcgtga tcagcaccga taacaaaaat
     5161 tgcatccggt aaaccgttca tatctttgat accgcctaag cttaactcta atttttctaa
     5221 ctcacgagta cgcattaacg cttctttttt agtgattttg tcaaaagtac cgtcttgagt
     5281 ttgagtttct aaatctttta agcgtttgat tgattgacga actgttttcc agttagtcaa
     5341 cataccacct aaccaacggt ggtttacgta gaattgttgg ctctctactg ccgctgcttt
     5401 aacagcatca cttgctgcac gttttgtacc aacaaataat actttaccat tgttactagc
     5461 aatgcgagtt aattccgcta atgcttcgtt gaataaaggt aaagtttttt ctaagttgat
     5521 tacgtgaaca ccattacgag caccaaagat gaatggtttc atttttgggt tccagtaacg
     5581 tgtttggtga ccatagtgaa cgcctgcgtt aagcatatcg cgcatagaaa cttgtgccat
     5641 aagaattttc cttcaaagtt gtttgttttg ccacttatga acggcaaaac ggggtttagc
     5701 ctccacatat cctagaaaat caatctgtta aagacacccc aattcctagc ttgtgatatg
     5761 tgtgtgtttt agtgttaaaa aataaatgtg tacgttttgc ctgaatagac aaaaacgggg
     5821 cgtattatgc caaaaaacaa acaatttgcc aattatttta ttgctctctt cgcattacta
     5881 aatcaagctc aaaaataaaa accgttctca ttattgacta aaaaaatggg gaggtttaga
     5941 atgtctttca ttctgttatg aaaacctgtc tgaaggagac tatcatgaaa aaaacacttt
     6001 ctgcacttgc tattgctgca gccactttta catcaacttc aacgcttgct gcaaatgaag
     6061 tgaatgttta ttcttaccgc cagccttact taattgagcc aatgctaaaa aactttgaaa
     6121 aagatactgg cattaaagta aacatcatct tcgctgataa agggttggtt gatcgtgtaa
     6181 aacaagaagg tgaattaagt cctgctgatg tgcttttaac agtagatatt tcacgcgtaa
     6241 tggaaattgt taacgctgat cttgcacaaa aaattgactc taaagtgtta gaaaaaaata
     6301 ttcctgcaca attccgtgac tctaacgacc aatggtttgg tttaaccaca cgtgctcgtg
     6361 ttatctacac gtcaaaagat cgtgtaggta aattaccagc aggctttgac tacctagatt
     6421 tagctaaacc agagtataaa ggtaaagtct gcgtgcgttc aggcaaaaac tcttacaatg
     6481 tctcactatt cgcggcgatg atcgaacatt acggcattga aaaaaccaaa gcattcttag
     6541 aaggcttaaa agcgaactta gcacgaaaac cacaaggtgg tgaccgtgat caagtaaaag
     6601 caattaaaga aggcatctgt gattactcta tcggtaacag ctactactac ggcaaaatgt
     6661 tagatgatga gaaacaaaaa tcgtgggctg aagcagcgat tattaatttc ccgagtggtg
     6721 aacacggcac gcacaaaaac atcagcggcg tagttattgc aaaacactca ccaaataaag
     6781 caaacgcggt gaaattaatt gaatacctaa gtggcgaaaa agcacaaggt ttatatgctg
     6841 aacttaacca cgaatatcca gtaaaagaag gcatcgagcc atcagcaatc gtaaaaggtt
     6901 ggggtacgtt caaatcagat accatcaaac ttgaagatat cgcaaaaaat tacgaagccg
     6961 cattaaaatt agttgatgaa gtgaaatttg acgatttctc tgagaaaaaa taatcaaccg
     7021 ataagcggtc atttttccac aaaaatttgc aaaattttgt taaaatacaa ccgcttgcaa
     7081 aaggacgcat tttgcgtcct tttatttttt tatgaaataa caggcttaaa gaaaaatgcg
     7141 atcacaattt tgctggaaaa gtattgctgc cctaacaact tttatcgttt tgcttcctct
     7201 tattgcaatt tgctatcacg cattgcaagg ggataatgaa aaccttgctc acctttggca
     7261 aacaatgttg ccaatctatc ttaaaaactc aactttatta gtattaggaa ccgttttcct
     7321 tgcactgatt tttgctctgc ctaccgcttg gattgtggca aattaccgtt tttgggggca
     7381 aagaacctta caatggttac tttgtttgcc tctcgctatg cctgcttatt taattgccta
     7441 tttatatacc gatttattag attactcagg tgcttttcaa ggctggctgc gtagtatttt
     7501 tggctggact catccgcaag attactggtt tcctcaaatt cgtacattgt atggtgcttg
     7561 ctttgtgctt gcgttagtac tatatccata cattttttta ttagtgcgag ttgccttatt
     7621 agaacaatct gaaaacttaa cacacagtgc aaaaatgctc ggggcttcgc cctctcagtt
     7681 gttccgtcga attacgctgc cattaattcg accttctgtt gccgtaggca ttgcattagt
     7741 ggcaatggaa acgcttggcg atttcggtac tgtggcctat tttgccgtgc caacacttac
     7801 tacggcaatt tatgattcgt ggctaggcta tcacgatctt ggtactgcca gccagattgc
     7861 gatctttatg ttgctaatga ttttcctgtt tttaagttta gagcaatata gtcgccgtaa
     7921 acaaaaaagc tatcaacgtg gctatgaaaa gaaatcagcc tttaaatttt taactggttg
     7981 gaaattagca ttagcccttg cttggtgctg gggtttattg gcgttagcct ttttcattcc
     8041 gtttggcagg ttgctctatt gggcatacga ctatttcgag caagcgtgga atttagactt
     8101 tgtccaattt tcgcttaata gtttgaaagt atcgatcatc gcctccgtga taacggtact
     8161 gattgctcta cttctgcatt tttctaaacg tttgaatacc cgcaaaattt tagtcaaaaa
     8221 tgaccgcttg tcattacaac ttgcctcact gggctatgcg gttcctggca cagtattagc
     8281 gatcggttta ctttcgcctt taacatttgc cgatcatcaa ctccatgctt ggctaaaaag
     8341 ccttgaatta tcacccgttg gattgatttt ttcaggctca ctctttgcat tagtattagc
     8401 ttataccatc cgatttttag caatggcaat tggcagcctt gaaacttcgc taaataaaat
     8461 ctctccatcg ctggatatgg cgagccaaac gcttggtaaa aatggctcag caatgttagt
     8521 aaaaattcat atccctttaa tttataaagg tattttaact gcaggattaa tggtctttat
     8581 tgagagtatg aaagagctaa atgcttctct gctacttcgc ccatttaatt ttgatacact
     8641 cgcaacctat gtgtttacct ttacatcaga cgagcagctt gagcgtgctg ctctgcctgc
     8701 catcgtattg gtgttagtcg gtttactgcc tgttatttgg cttaccagat cccttatttc
     8761 gcaatctgaa aaagaaagat aaaggaattt tatgtctgtt ttaactatta atcatctcaa
     8821 cctcaatttc ggcagtacaa aggttttaca aaatctccat cttgctgtga atcaaaatga
     8881 aattatctgt ttgctaggtg caagtggctg tgggaaaact acactgctca aagccattgc
     8941 aggtttattg ccaattgagc agggagatat tcatttagcg ggtgaatgcc taaaaagtaa
     9001 agccgtggaa gagcgacaaa tcgggctgat tttccaagat tacgctcttt ttccacactt
     9061 gacggtagcc gataatattc aatttggctt atccaaattg ccaaaagtac aacaaaaagc
     9121 aattaccgaa aaaatgcttt cagttgtgca tttacaaggc tttgaaagcc gttttccaca
     9181 cgaactttcg ggcgggcaac aacagcgtgt cgctattgcc cgagcattag cctgccagcc
     9241 agaattgcta ctgttagatg agccgttttc caatattgac agccaaactc gctatgcaat
     9301 gattcaagag atcaagcaaa tcttaaaaag ccaaaatgtg cccgctattt ttgtaaccca
     9361 tagcaaagaa gaagcctttg catttgccga taaaattgcc gtgatggatc aagggaaaat
     9421 tattcaattt ggtaagccaa acgagctata ccattctcca gttaatccat ttgtggcaga
     9481 ttttttaggt ggcacaaact atcttaactg taatgtaacg gaagacaacg tgttatgcag
     9541 ccctattggc gagttccgac tcttccctga aatgaacgtt cagaaagggc gttaccgttg
     9601 gcttcttcgc ccagaacaga ttatcttaaa acccaatgag ctaggtttag gtaaagtggt
     9661 aggcaaactg tttttaggcc tatactaccg ctatcaggtt gaaattaaag gcattgagat
     9721 agtaacttac caatctcaag agctgaaaat tggcgaacaa gtggaagtgg gcttccaagt
     9781 aagagagttt atcctatttt aaggagaata tatgaaaatt gaagtttggt cagattatgc
     9841 ctgcccgttt tgttacatcg gcaaacgcca cttagagcaa gccctagccc aatttgaagg
     9901 cgaggttgaa gtggttttcc gagcctttga gcttgatccg cacgctaacg gcgaaccgga
     9961 gggcgatatt cagcaacgcc taatgcggaa atatcaaaaa accgcagaac aagctgatga
    10021 aatgattcgt tatgtggaac aagccggcaa acaagctggg ctagatttgc gttaccgcac
    10081 cacccaatac actcgcacct ttgaagctca tcgcttggcg aaatttgcag aaagtaaaga
    10141 cttaggcgaa gcaatggttg aacgtttatt caaagcctat tttaccgata acactattct
    10201 agctaaacgc acagagctta tcagtttagc cttagacatt ggcttagagc gtgatgaaat
    10261 cgctcaactg ctcacaggtg atgatttcgg gcacgaagtc cgtgaagatg agcgagtcgc
    10321 tcacaaatat ggcattcaca gcgtaccgtt ttttgtgatc aatgaaaaac tcggggtatc
    10381 cggggctcaa ccgccagaaa tattacttga cgctatcaaa caggcattgc aaaaataatc
    10441 cgaaaaaaga ccgcttgtta agctaaacaa gcagtcttat tcttcttata atttacaatt
    10501 tattttttgg tgatcgcacc taaaatacca cgcataattt gtttggtgac ttggttgcgt
    10561 aaatttctgc caactgaatg cgccacttgg ctaaccactt gttcggtaat cgattgatct
    10621 tttttcttct tcgagccaaa aatggaagag agaataccgc caaagaagtc attgtttgct
    10681 tcttctgccg cagcctgctc tgctttaatt gcttctgctt tcgcattaag taattcaaac
    10741 gcagattcat tatcgacata ttggctgtag tgatgataga gatcatcttg cttcactaaa
    10801 ctatcacgtt cgcctgtggc taatggagct aattggcttt ttggtggata gatataggca
    10861 atttctacag gcgttggcat acctttttca tctagcaccg aaaccaacgc ttgccccaca
    10921 ccaagttgcg tgatggcttc taccacattt acctctttat tcgctcggaa agtttccgct
    10981 gctgatttta ccgctttttg atcacgtggc gtgaaagcac gcaaagcgtg ttggatacga
    11041 ttgccaagct gccctaatac tgaatcaggt atgtccaacg gattttgcgt aacaaaataa
    11101 acacccaccc cttttgagcg aattaaacga accacctgtt caattttatc caccaatgcc
    11161 ttaggggcat cgtcaaacaa taaatgagct tcatcaaaga ataaaacaaa cgtcggtttt
    11221 tctgggtcgc caacttctgg caaattctca aagagctctg ccataaacca aagcaggaat
    11281 gcactgtaca ctcttggtga gttaattaat ttttctgcat tcaacatatt gattacacca
    11341 cgcccattac gggtttggat ccaatcgtgc aaatctaatg caggctcacc aaataaatta
    11401 tctgcccctt cattttcaag ccttaataac gcacgctgaa tagctccaac gcttgctgca
    11461 gaaatatgcc catattccag ttggaattct ttcgcattat ctgcgacaaa tttaagcaac
    11521 gcacgcaaat cttttaaatc aattagcaat aaacctctgt catctgccac acgaaaaacg
    11581 agatttaaca agcctccttg ggtatcgttt aaattgagta aacgagaaag taacatcggt
    11641 cccatttcgg aaatagtgat tcgaagtggg atacctgatg taccaaatac gtcccaaaac
    11701 gaaactggat agccctttaa gtaatcttct ccccctaaat taaactgtaa aatgcgttct
    11761 gcaatttttc ctgaaaaatt acccgcttgt accgagcccg aaagatcacc ttttacatct
    11821 actaaaaata ctggcacgcc atcatcactc aaagattctg ccaatttgcg taaagtaacc
    11881 gttttacctg tacccgtagc cccagcaatt aaaccgtgcc gatttgccat tttggcatta
    11941 agcgaaataa ctttgcctgt ttctgttcga gcaatttgat acaaactcat ctttcattct
    12001 cttaaaggta aattaatttt ttgttctcat catataaaaa tttatcactt aattacggct
    12061 attctttttc gtttttgcca agaacgtttt tttacttctt gagcggttca ctttcggtaa
    12121 agtcagtaag gattccttgt cgtattgact cacaggaatc tgatgcccaa tatactcttc
    12181 aattgcaggc aaattaaccg cataacgctc gcaagcaaaa ctaattgaat ggccgctctc
    12241 ccctgctcgc cccgttctac caatacgatg aacgtaatct tctttatcat caggcagatc
    12301 ataattgaaa acgtgcgtga catctggaat atgcaatcca cgagccgcaa catctgttac
    12361 cactaaaata tccaaattgc ctttggtgaa attatccagc aaagaaaggc gttttttctg
    12421 cggaatatcg ccggttagca ttccaacccg atgtccgtct gccgataaat actgccagat
    12481 ctcttcgcat ttatgcttgg tgttggcaaa aatcatacaa cgctccggcc actcttcctc
    12541 aagcaatgtc agcaacaatg ccattttgtc ttcattcgag gggtaaaata gctcttcttt
    12601 aatacgatgt ccggtttttt gtagtggctc aatttcaata tattccgcat tatccatatc
    12661 ttcataggca agttcccgca cttttgccga aagcgttgcc gaaaagagca aagttaaacg
    12721 ttctgaaggc ttaggtgctt ttcgcattaa ataacggata tctttaataa aaccaagatc
    12781 aaacattcga tctgcttcat ctagcacaat cacttgaatt ttgtctaaat tgataatgcc
    12841 ttgttttaca tagtcaatca ctcgacctgt tgtaccgaca agaatatcta cccctttttc
    12901 aatttcttga acttgtttgt cgtaaccatc accaccgtat gccaaagcta gttttaaatc
    12961 agaatgtttt aaaaacagct cggcatctgc cccaatttga accgccaatt cacgggttgg
    13021 agcaagaatt aatgctctcg gctgattttt tgacgtttcg atttcgttat tcaataaatg
    13081 gttaaaagtt gcaatcaaaa aggcaagcgt tttccctgta cctgtttgag cttgccctgc
    13141 aatatctttg cctttcagcg taatcggaaa ggtttttgcc tgaatcggcg tacaaaattc
    13201 aaagcctttg ctatcaaggg catttagtac attagcgtga atcgggaagt caataaagcg
    13261 gtgttcggta agatgattag acatagtatt tccttgataa ttgtgatgtg acaagcggtc
    13321 ttttttacac atttttttgc aaaatagcat attgggcgtt ttttctcaaa tttaaccgct
    13381 tttgagttta ttcggcaaaa tagattggaa aagctctcgg cttttcagcg gttgtgaccc
    13441 taaatagggc tttagtgcca ttcgggtaaa acgtttcgct gcttgttgag tacttttttc
    13501 tgaaaaatcc aactgagcaa acgctaataa atcactgcct aaaaagctct ggttattctg
    13561 taataatgag gcgataaagc cctgattttc ctgaaattga taggtcattt tcggatcaac
    13621 tggcaaaccg gttgccgcac agtgcgtaaa atccacccca taacccaatg cttttaacgt
    13681 atgaaattca aaggtgcgta aaatcggctc aatctgtttt tgtgtggcaa gctcggtaat
    13741 gcaacgtaaa taatgctgaa ataattcagg gtaagcggtc tgattttcca atactcttgc
    13801 aagtacctcg ttgatataaa acccactata aagtgaaagc gtttccatcg gcaacgttaa
    13861 tgcagccggt tctgctttgg ttaaagtttt cagatcccct ttacccgacc aacgcaacaa
    13921 caacggcgtg aacggctgta aaaccgcttt caacggcgag cgagcccgcc ttgcaccttt
    13981 agcaagtaaa gtaatccgcc cgtgatgttc ggtaaaaaac tccactaata aactgttttc
    14041 gctgtattca cgccgatgaa ggacaaaggc tcgttgccat tggtctattg tcataatttc
    14101 ttgttaatta tgaggttgta atttacttct gccaaatctc cccctcccct ctttgctaaa
    14161 gaggggagat cttgcagagt atattttaaa gtcattttca ctatttaacc ttttatatag
    14221 ataggaagat gaatttaaaa tttctggaga actagtcccc ctctttagaa aagaggggtt
    14281 aggggagatt tgattacgat attaatgcta taacgggagc caactactcg tccatatacc
    14341 ctaaacttct caatgctctt tcatcatccg cccaaccagc ttttactttc acccaaagct
    14401 caaggtgcac ttggttgtca aataggcgtt ccatatcttt tcgagcttca atgccgatgg
    14461 ttttgatttt ttgccccccc gcaccaataa ccattttttt ctgaccatct cgttctacta
    14521 aaatcaagcc attgatttca tacaccccac gctcattcat tttaaattgt tcgatttcta
    14581 ccgttaccga ataaggtaac tcttcacctg taaaacgcat tagtttttca cgaataattt
    14641 ctgatgccat aaagcgttga gagcggtctg tcacatactc ttcaggaaag tggtgctcac
    14701 cttgacgcag tgattgacgg acaaatttct gtaaaatatg cacattttta ccccgttgtg
    14761 ccgaaattgg cagaatttca gcaaaatcaa atttttgtga aagctcggta atatgcggca
    14821 gaagttcttc tttttccttg atgttatcaa ttttattgat tgccaataaa acaggggctt
    14881 ttgccgaacg caatttgttt aacaccattt catcatcttc tgtccatttt gtgccttcca
    14941 caacaaagat gatgagatct acatcgccaa tagcagaact tgctgcacgg ttcattaaac
    15001 gattaatcgc acgtttttct tctatgtgta agcctggggt atctacataa atcgcttggt
    15061 attgatcttc agtgtgaata ccaacaatac gatggcgagt cgtttgagct ttgcgtgagg
    15121 taatggaaat tttttgtcct aaaatcttat ttaataatgt tgatttccct acatttgggc
    15181 gaccaacaat cgcaataaaa ccacaatagg tttttgcagg agtttgaatt tgttctgtca
    15241 ttttgttttc ctttcattct gtggaatgaa tttgatggtg caagcgtcca cgcttgtacc
    15301 atttaattaa atctttagca caagcgagga cgcttgcgct atcttacttt ttcgttttaa
    15361 ccaccgctaa cgctttttct gcggcatttt gttcggcttt tcggcggctg gtgccaatgc
    15421 cgataaacac ttcctcgacg ttaatcactt tacaggttac acggaaagtt tggttatgag
    15481 cttcgccttt aatatctaat acatcatatt caggcaatgg caattttcta ccttgtaaaa
    15541 actcttgcag gcgggttttt ggatcttttt gtgaatcgcc tggactgata tctttaagca
    15601 aatctgaata ccaatcttcc accttttcca ttgcttttgc catatcttga tcgagataaa
    15661 tggccgcaat aatcgcttca acacagtccg ataaaatcga ttctcggcga aaaccaccac
    15721 tttttagctc tcctggacct aattttaggt agtcgcctaa atcaaatttg cgagcaataa
    15781 tcgctagagt ttgctcacgc acaagagttg ctctcatacg gctaagctca ccttcattcg
    15841 cttttggaaa tttgtcgtat aatactttac caatagtaaa atttaaaatt gaatcgccta
    15901 gaaattcaag acgctcattg tttttggttg ccgcactgcg gtgtgttaag gcctgaatga
    15961 ggtaatcaag gttatgaaat tgataaccta attttttctg taatcgttct agttgcatag
    16021 tttatgtgaa taaaggggcg tatctcatac gcccctgtta agattaattg attgaggtaa
    16081 acattctaga aaaacgaagc cccgttggga attcatttgc ttttttatct aaacttaacc
    16141 aaataaaagt ggctttacca acgatgtttc tctcaggcac aaagccccag aaacgactgt
    16201 cttcactgtt atcacggtta tcacccatca caaaatagta tccttgtggc acaacccatt
    16261 caccttctgc ttgcccttct tgttggtaga aataaggtgc atagttaaat gccattgggt
    16321 gatttaaaat ttgatgagtt acattacctt tttcagtgcg ttctacctgc atttcaccgt
    16381 gatagaaaaa ctcaggattt ggcttgcctt cgctgtattc aaataccttt gtttcgccat
    16441 tggctttagt aaccgttaat tgctgagttg caaaatcata tttaatttta tcaccaccta
    16501 ctgccaccac acgcttaata taatcaacgt gtggttgtgc cggtgctttg aatacaatca
    16561 catcgcctcg ttctggtttg cccgtttcaa tcaaggtgtt ttgccagata ggatctttaa
    16621 tgccgtagct gaatttattt acgactaaaa aatcgcctac acgcaaggtt ggctccattg
    16681 aaccacttgg aatttgaaac ggttcaaata agaacgagcg aagtactgta acaaagaata
    16741 aaatgccgaa aagtgaggca aaaaattcgc ctactgcgga tttaggttga atttccgcta
    16801 tttcttcagc ggttaattct ctgcctaaac gtttttgttg tagaacaatc gcctgtttac
    16861 gtcttgggtc agcactgaat ttgtaaaacg cccaaaatcc accacaaata ataaccaagg
    16921 caactaaaat aatggaaata gtatttggca gttgcattga atcaagccat tgccaaatac
    16981 cataaataat acctagaaaa ataattggca taaattgtgc cactgaatgc tcctttttat
    17041 tcaaaattgc aaagttttct caaaatatga ccgcttgcaa gcggtcgtat tttacacgaa
    17101 tattgacaat taatccttac ccacatgaag aattgccaaa aatgcttctt gtggcacttc
    17161 tacgttaccg agagatttca ttcgtttttt accctctttt tgtttttgta gcagtttctt
    17221 cttacgactt acatcaccac cgtaacattt tgctaatacg tttttacgca actgttttac
    17281 cgttgagcga gcaatgacgt ggttaccaat tgccgcttga atagcgatgt caaattgttg
    17341 gcgtggaata agttcacgca ttttttccac taattcacgt ccacgatacg ccgcatttgc
    17401 acggtgaacg ataatcgcca acgcatcaac acgctcgccg ttaatcataa tatccacacg
    17461 caccatatca gccgtttgga aacgtttaaa accgtaatcg agtgaagcat aaccacgaga
    17521 ggttgatttt aagcggtcga agaagtctaa cactacttcg cccattggga tttcgtaagt
    17581 taaggcgatt tggttaccgt gatagaccat attggtctgt acgccacgtt tttccacaca
    17641 aagggtaatc acgttgccta ggaattcttg tggcacaagc atattacatt ctgcaatcgg
    17701 ctcacgaatt tctgcaatat tgttgattgc tgggagtttc gatgggctat ccacatagat
    17761 cgtttcgcca ctggtttgca ccacttcgta aactacggtc ggagcggtag taatcaaatc
    17821 aagatcgtat tcacgctcta aacgctcttg aatgatctcc atatgcaaca agcctaagaa
    17881 gccacaccgg aagccaaagc ccaacgcact tgaagtttct ggctcataga acaatgacgc
    17941 atcatttagg cttaatttgc ctaaagcatc acggaaggct tcgtagtcgt ctgaactaat
    18001 cggaaataac cctgcataaa cctgtggttt tactttctta aagcccggta atacttcagt
    18061 tgctgggttg tggtggtggg ttaatgtatc gcccactggt gcccctaaaa tatctttaat
    18121 cgcacaaact acccaaccta cttcacctgt ttttagctcg gtagtatcca cttgttttgg
    18181 cgtgaaaata ccaaggcgat ctacattgta agactgccct gtactcatca ctttaatttt
    18241 atcgcctttt ttcagcacgc catttttcac acgcactaaa gacacaacgc ctaaatagtt
    18301 atcgaaccaa gagtcgataa ttaacgcttg taatggtgct tccgcatcgc cttctggtgc
    18361 aggcattttc ttcacgattt cttctaaaac atcttcaatg cctaaacctg ttttagctga
    18421 acagcgtact gcatcaatgg catcaatgcc gacaatatct tcaatctctt ctgccacacg
    18481 ctcaggctcg gctgctggta aatcgatttt atttaaaatt ggcacaacct ctaaatctat
    18541 ctcaattgcg gtataacaat tcgccaacgt ctgtgcttcc accccctgcc ctgcatcaac
    18601 cactaataaa gcaccttcac aagcagcaag cgagcgagaa acttcatatg agaaatccac
    18661 gtgccctggc gtatcaataa agttgagctg ataaatttcg ccatctttcg ctttatagtt
    18721 taaggttaca ctttgtgctt taatagtaat tccacgctca cgctcaagat ccattgaatc
    18781 taatacttga gcctccattt cacgatctga taagccacca caagtttgaa ttaagcggtc
    18841 tgataaagtc gatttgccgt gatcgatatg agcgataatc gaaaagttac gaatattttg
    18901 cattttcatc gggcagttaa tgtcctgttt tgttatttgt ttatcaaaaa ttaatcgggg
    18961 aattgtactt gaaatacaag gttttgcaaa gaaaacaaat agcaataagc ggttattttt
    19021 tcctaaaaat gtgcaaattt cttga
//
LOCUS       DANLUA010000024        18880 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLUA010000024 DANLUA010000000
VERSION     DANLUA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18880
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            51..2486
                     /gene="plsB"
                     /locus_tag="PXB44_000658"
     CDS             51..2486
                     /gene="plsB"
                     /locus_tag="PXB44_000658"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL5281169.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(2591..3154)
                     /locus_tag="PXB44_000659"
     CDS             complement(2591..3154)
                     /locus_tag="PXB44_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL5281170.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(3352..4248)
                     /locus_tag="PXB44_000660"
     CDS             complement(3352..4248)
                     /locus_tag="PXB44_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5281171.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            4384..4725
                     /locus_tag="PXB44_000661"
     CDS             4384..4725
                     /locus_tag="PXB44_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5281172.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            4736..5089
                     /locus_tag="PXB44_000662"
     CDS             4736..5089
                     /locus_tag="PXB44_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5281173.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            5149..5787
                     /locus_tag="PXB44_000663"
     CDS             5149..5787
                     /locus_tag="PXB44_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL5281174.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            5922..7103
                     /gene="purT"
                     /locus_tag="PXB44_000664"
     CDS             5922..7103
                     /gene="purT"
                     /locus_tag="PXB44_000664"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL5281175.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(7195..8688)
                     /locus_tag="PXB44_000665"
     CDS             complement(7195..8688)
                     /locus_tag="PXB44_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5281176.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            8889..10736
                     /gene="ilvD"
                     /locus_tag="PXB44_000666"
     CDS             8889..10736
                     /gene="ilvD"
                     /locus_tag="PXB44_000666"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL5281177.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(10870..11220)
                     /locus_tag="PXB44_000667"
     CDS             complement(10870..11220)
                     /locus_tag="PXB44_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL5281178.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(11249..11914)
                     /locus_tag="PXB44_000668"
     CDS             complement(11249..11914)
                     /locus_tag="PXB44_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL5281179.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            12120..12668
                     /gene="pgsA"
                     /locus_tag="PXB44_000669"
     CDS             12120..12668
                     /gene="pgsA"
                     /locus_tag="PXB44_000669"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL5281180.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            12746..14584
                     /gene="uvrC"
                     /locus_tag="PXB44_000670"
     CDS             12746..14584
                     /gene="uvrC"
                     /locus_tag="PXB44_000670"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL5281181.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(14589..14864)
                     /locus_tag="PXB44_000671"
     CDS             complement(14589..14864)
                     /locus_tag="PXB44_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281182.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(14989..15342)
                     /gene="rplT"
                     /locus_tag="PXB44_000672"
     CDS             complement(14989..15342)
                     /gene="rplT"
                     /locus_tag="PXB44_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL5281183.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(15451..15648)
                     /gene="rpmI"
                     /locus_tag="PXB44_000673"
     CDS             complement(15451..15648)
                     /gene="rpmI"
                     /locus_tag="PXB44_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL5281184.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(15912..16454)
                     /gene="infC"
                     /locus_tag="PXB44_000674"
     CDS             complement(15912..16454)
                     /gene="infC"
                     /locus_tag="PXB44_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL5281185.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(16613..18412)
                     /gene="recQ"
                     /locus_tag="PXB44_000675"
     CDS             complement(16613..18412)
                     /gene="recQ"
                     /locus_tag="PXB44_000675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL5281186.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRNKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(18521..18826)
                     /gene="cyaY"
                     /locus_tag="PXB44_000676"
     CDS             complement(18521..18826)
                     /gene="cyaY"
                     /locus_tag="PXB44_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL5281187.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         5484 a         3603 c         4138 g         5655 t
ORIGIN      
        1 gaaattagag gttagacctc caacaataac gattctaaaa ggatatttat atgtctggct
       61 tattaaattt ttatcgagct ttcctaaact taccactttc acttttagta aaatctcgct
      121 ctattccgac tcatccggtg gctgaactgg aactaaatct tgaacagccg attgtctatg
      181 ttctacctta tacctcgcaa accgatttat taattttaca aaaaaactgc ttggctttga
      241 atttgcccga tccgttgcaa gaaaatgtga ttgaagggca aacattgctt cgttttgtat
      301 ttttagatga aggtcgccgt ttttttaaat ctaaaggggc gaaaagtgag acagagtcga
      361 ttttttatcg ttatttagat ttacatcgtg ctaatgctga attagacgtc caattagtgc
      421 cggtttcggt attatggggg cgggctccgg gcaaagaaga tgcccgccat ttacaggtgc
      481 ttacctcatt ccaacgtttt ttatccatgg tttggtttgg gcgtgataat tttgtacgtt
      541 tctcccctgc agtatcgtta cgctatatgg taactgaata tggtgcagat gaaaaaatcg
      601 ctcaaaaaat cgcgcgtgtg gcgaaaatgc actttgcaaa attgcgttat tcagcaatgg
      661 ggcctcgttt accaaatcgt gatgcgatgt ttaataaaat cctgaattca gaggtgattc
      721 aagctgcgat tgcggaagaa gcgaagaaat catcgcctga aaaggctcgc aaagaagccg
      781 agaaaatcat taatgaaatt gccgctgatg taaaacacga gagtttgcgt gtagcagatc
      841 gagttctgag ctggctttgg aataagcttt atcaagggat caacgtgcaa aatgcagacc
      901 gtgtgcgtaa attggcatta gaaggtcacg agatcgttta tgtgccttgc caccgtagcc
      961 atatggacta tttgctccta tcttatctac tttaccatca aggattagta ccgccacata
     1021 ttgccgctgg gatcaattta aatttctggc cggcaggccc gattttccgt agttggggag
     1081 catttttcat tcgccgtacc tttaaaggaa atcgcctcta ttccaccatt ttccgtgaat
     1141 atttagcgga attattctat cgtggttatt cggtcgaata ttttattgaa ggcgggcgtt
     1201 cacgcacagg tcgtttatta gagcctaaaa ccggtatgat gtcgatgagc ctacaagcct
     1261 tacagcgagg gctgaataga tctttaagta ttgtgcctgt ttatatcggt tatgaacacg
     1321 tcttagaagt cgatacttac gctaaagagt tgagaggtgc ggcaaaagag aaagaaaatg
     1381 cgggcttggt gctacgagtg ctgaaaaaac tgaaaaattt aggtcaagga tatgtgaatt
     1441 tcggtgagcc aattttggtg aataattact taaatcaata tttccctgag tggaaagagc
     1501 cttccgaaga tggacgacct aagtggttaa atgaagccgt ggataatatt tcacatcaag
     1561 taatggtgaa tatcaataaa gcggcagcag ttaatgcgaa aaacttagtg gggtcagcat
     1621 tattggcatc tcgccagcgt gcattaaccc gtgaacaact cattgaacaa cttggaagtt
     1681 atattcagtt atttcgtaat gtaccttatt ctaaagatat gacattacca actgaaagtg
     1741 cagaagtgat gttgaatcat gttattcatt tacctcgttc gggcgtttta attgaaaaag
     1801 ataattttgg tgaactggtt cgtttagaac gagaatctgc tgtgttaatg acatattaca
     1861 gaaataatgt gcagcattta tttgtgttgc cttcacttgt tgcaagtatg gtacttcatc
     1921 atgaggctgt ttcgaaagat gttgtgatta agtcggttaa tcgtatttat ccattcttgc
     1981 aagcagagtt attcttacac tttaagcaag aagaattaaa agcacatatt gaagcaatta
     2041 ttgcggaact aagtcggcag ggggtgatta aaaacgaaag tgatatgtta atgatcaatc
     2101 gctcacatat tcgtaccttg caattacatt cagctggtgt gcgtgaaatc cttcaacgtt
     2161 attatattag cctaagtgtc ttgttggagc agccaaatat tagtcgtggc tatttagaaa
     2221 aagagagtag ttcaattgct cagcgtttat cagtcttgca tggtattaat gcgccggagt
     2281 tttttgataa ggcgattttc tctaccttta catcaagtct aaaagcacaa gggtattttg
     2341 atggtgatga taatttagcg attgcaaaag ttaaagaaac ggaatcgttg ttacgtagtt
     2401 tgatttcgat tgaagttcaa cagaccattc aaggtgcgat ggctaaagtc gcagaatctg
     2461 tagagaatcc taatatcgca ggataataaa tcaaagaagg gcgctaacaa gcgctcgttt
     2521 cttgcaattt tgttgccaaa aataaaagct gaatgcgcga ccacattcag cttttttact
     2581 tcctatataa ttattttggt tcaccgattg gtaaaacagt cttaccaaaa ctttcattaa
     2641 taatatccgc agcggctaag tagaacgcta agaatgcggt taataaaccg aagttaccac
     2701 caatgttagt tagggtggtg ttatgggtaa aatcacctgc ggctaaaagg tagaaagtaa
     2761 tggttaaact taagaaaata gcttgtaatg cacgggcttt tgctagtgtg ccgatgaaca
     2821 tcataagtgt gaatgttccc caagctacta agtaccaacc aacgaattct gccgatacat
     2881 tcgcagcaaa gaacacttta aataatgcaa aagaccacca gaatgcacca tagctggtaa
     2941 acgcagtaaa gccgaacgtg ttacctttag caaattcaaa catacctgca atcatttgtg
     3001 atgtgccacc gaaagcaaat cccattgcta aaactaaacc tacgttttca ccgctgaatg
     3061 taccgttatt gattaggctt agtaaaaatg tagtaagtgc aaaaccgcat aaaccgagcg
     3121 gagccgggtt agttcctgaa tttggagctg acattgaaaa cctctgttat attgttacac
     3181 cataagtgtg gtaattgttt atttacgccg ataaatcaga cgttatgtgc ctaaaattta
     3241 gggcgataaa gtatagcgat tttgcagtaa attgactaga aatttccgag acaagcggtg
     3301 agatttttgc aaaattttgc caaaaaatga ccgcttgttg ttgtcattta gctatggatt
     3361 taactgccga tattggctgg gtgttacgca atattgcttt ttaaaggcgg taataaaatg
     3421 cgactcggat tggaagccgc aagataaggc gatacttaat accgacccag cttggctttt
     3481 gagcagcatt gctgcttttt gtagcctaat tttgtgtaca aaagcatgtg ggctaataca
     3541 taaatgtttg ttaaataagc ggactaactg ggcacgagaa aggtggataa atgccgccat
     3601 atcatcgact tgccattcgt gttcgggatt aagcaaaata gctttgatca aaggtcttaa
     3661 gtgtaagtct tgccaacctt ttaaaatacc gaataactga ttgttatggt taactagatg
     3721 ttctcgtaag atcatagtta aaataacaac agcaagcgag ttaaccacag attttacggc
     3781 taaattaggt gtttctgctt cttgttttag aatatctaac attgctttta atggtgtctc
     3841 ggcaacttga aatacgatca tttcaggtaa gctttccatt aattcggctt gatggtcata
     3901 ataaaaatgg aggcagaaca tatcgcattc tgattgagaa agtcctgctt gggtgagtgt
     3961 aaatgcaccc aattgttcag ggtttaccgc atttttgctg gtcaaaggct gtatttgact
     4021 acgtaatgta tgatcggcaa atctggggaa aaatacaata tctccctgtt taaggtgatg
     4081 ggttttccca ttatgttgta gataagcttg cccatagctg atgatatgag cgattgcttg
     4141 tccggtttga tgcggattct caagttgcca atctccctgt aactgacatt tcacactgat
     4201 tccggctgag atttgagcga aagctagaag cctatctaat atatccattg ttcattcctt
     4261 atatttccac cttaaattgc gattatttca ttataatatg agctgatttg ataataaagt
     4321 ttatcagata aacgcaataa taaatgcgtt ttttcatatt attcacttta ggaggaaatc
     4381 aatatgtctt cattccaaaa ttggacagaa cacacagctc acgttaagca atcttttggt
     4441 aaattagggc aaaaacaccc taaaatgctt gaagcttata aagcattagg tacagcggcc
     4501 gcagcggaag cattagatca caaaactcgt gaattaattg cattagcagt agcgattaca
     4561 acacgttgtg aaagttgcat cagtgtacac gcagaagaag cggcaaaagc aggtgcaacc
     4621 gaaagcgaaa ttgccggtgc attagcgact gcgattgcat taaatgccgg tgcggcatat
     4681 acctatgctt tacgtgcatt agaagcggtg gaagagcaaa aataaggagc aataaatgaa
     4741 gaaattggct tggattttag gctttgcagc tatgatgcca atgatgtcta gtgctaaaca
     4801 agcatcagtt cagcaagtgc aggctgaaaa agcagcgggc atttggattg atgtacgttc
     4861 tgctgaagaa tttcagcaag ggcatttaaa tggtgcaatt aatgtaacgc atactgaaat
     4921 tgcacaacgt atcgcagaag ttgctccaga taaaaatcag ccgattcatc tttattgccg
     4981 ctcgggtcat cgagctgaag cggcgcttaa tgagctaaaa aaattaggtt acagcaatgt
     5041 aactaatcat ggtggttatg acgatctgat taaagccggg ttgaaataac tgttttgagt
     5101 gccaagcatt tgcttggcac ttttcgttta tatctgaggt taaaaattat gagtgctttt
     5161 aacaattttg ttaattttgt tgcaaatatt gttgctccaa tgcaacgcca attcattaac
     5221 tttgtacgta ttgccatttg tatcgtgatg gtttggattg gtggtttaaa agtctgccaa
     5281 tatgaagcag acggtattgc ccattttgtg tctaatagcc cgttctttag ttatatgtac
     5341 aaaaaaggcc cgaatttagt tgatgacggc acaggtaaaa tggtgatgga atataccctt
     5401 cacaaaaacc ctgaaggtaa aatggtggca aaaaacatcg aatggcataa agaaaacggc
     5461 acttatactg catcttatat catcggtgca accattgtga cggttggttt attgacgtta
     5521 tccggtattt ggttcccggt ttccggtatg gcggggggct tactcacctt tggtatgtcg
     5581 attgttacac tctcttttat gatcaccacc cctgaaattt gggtgccgaa tttaggtggg
     5641 gatatgccaa ccccagcgca tggtttccca tatctgtcag cagtcggtcg tttaattgtg
     5701 aaagatgtga ttatgatggc aggcggcttg gttgccgcag cggaatgtgc aaatcgcatt
     5761 ttagccagac gtaaagcgat tgcttaattg atagtggtaa tgcaagcggt cattttcttg
     5821 taaaattttg caataaaatg accgcttgtt ttttgtcaat ttgccataat ttcaagtata
     5881 attcaattac gtaatcgttt gcttttatgg agaggaataa aatgacaaca atcggtacac
     5941 cgttaagaac gaacgctaca aaagtgatga tgttaggttc gggcgagttg gggaaagaag
     6001 tggtaataga gctgcaacgc ttaggcgtag aagtgattgc ggtggatcgt tatgacaatg
     6061 cacctgcaca gcaagtggct caccgtgctt atacgatttc gatgttagac gggaatgcat
     6121 tgcgagattt ggtggagaaa gaaaaaccgg attttattgt gcccgaagta gaagccatcg
     6181 caactgcaac cttggttgag ttagagcaag agggatataa cgtaatccca acggcaaaag
     6241 caacgcaatt aacaatgaat cgagaaggca ttcgccgatt ggcggcagaa gagttgggct
     6301 taaaaacgtc gccttatcgt tttgtggata attttgaaca gttccagcaa gcgattcaag
     6361 aaatcggcat tccgtgtgtg gtcaaaccga ttatgtcttc ttccggacac gggcaatcag
     6421 taattaaatc tgaagcggat attcaacagg cttgggatta ttcgcaacag ggcggacgtg
     6481 ccggcggtgg gcgtgtgatt gtagagggtt ttatcaaatt cgattatgaa attacccagc
     6541 ttaccgttcg ccatattcac ggcacgtcgt ttttagctcc catcgggcat attcaggttg
     6601 atggcgatta tcgtgaatct tggcaaccgc agcaaatgtc ggacattgca ttgaaaaaag
     6661 ctcaagaaac cgcagaaaaa attacctctg cactgggcgg aagagggatt ttcggcgtgg
     6721 aactttttgt ctgtggcgat gagattattt ttaacgaagt ctccccacgt ccacacgata
     6781 ccggaatagt aacaatggca tcacaagagc tttctcaatt tgctctgcac gcccgtgcga
     6841 ttttgggctt accgattccg gaaatttacc gcatcagccc ggcagcttct aaagcgattg
     6901 tggttgaagg gaaatcggat aatgtgcgtt ttggcggtgt agataaggta ttagccgaaa
     6961 tcgggacgaa tatccgctta ttcggtaaag gcgaagtaaa cggacaccgc cgattagggg
     7021 taattcttgc tcgtgatgaa aatacagtga gagcattaga aaaagctcgg cgagcttatg
     7081 ataagttaga tattcaatta taaagtcagg attttgatat tcgttgatag taggcatttt
     7141 atgcctacta tcctataggc ctctaatcaa attggatgaa aaagttgaag attgttaatc
     7201 ctctttgccg agaatttggt tcacttcgct cttatacaat actgcttttg ctccaaatac
     7261 ggcttgtaca ccgccaccga cttccagcac gtcaatcgca ccgagttttt tcagtgttgc
     7321 tttatccacg gctttcacct ctttcactgc cacacgcaga cgggtaatac aagcatccac
     7381 attttcgata ttatttgcag agccaagagc ggcgataatt ttatgggcat tttcagtcag
     7441 tgagctggtt tgttgttcga ctacctcttc actctcttcc gcacgtcccg gtgtcattac
     7501 gttgaatttt ttgatcagga ataggaacga gaagtagtaa agtgccgccc acggcaagcc
     7561 gactatgatc actcgaagcc agttagtgtt ctcgttgcct tgcaaaatgc caaacagggt
     7621 gaagtcgatc acgccaccgg agaaggtgtt accaatcgag atgttgagta agtctgcaat
     7681 gtagaacgac acgccgtcta aaaaagcgtg gaacacatag agccacgggg caacaaacag
     7741 gaacataaat tcaatcggct cggtaatgcc ggtgataaaa gaggtgagcg ctgcacctaa
     7801 gaacagaccg ctaatcgcac cacggcgggc tttcggcaca gcgtgataca tcgctaaaca
     7861 ggcggcaggt aagccaaaca tcatcgtatc gaaacgtcca gcgaagaaac gtgtaccttc
     7921 ggtaaataag ccttgatggg ttggatcggc gagttgggcg aagaagattt tttgcgcccc
     7981 gataatggtt tcaccattta ccacttctgt tccgcccaat tcggtgtacc aaaacagcgg
     8041 gtagatcata tggtgtaagc cgaccgcacc gcttaaccgc attaagaaac cgtagaaaaa
     8101 ggtgccgatt tcgcccattt ccgcaatccc ttgccctgct gaaaccaacc actgttggaa
     8161 agccggccag atcaaaaaga acactgcccc gacaaaaatc gcagcaaagg cggtcacaat
     8221 cggcacaaac cgagagccgc caaagaagcc gagaatttgc ggcagctgaa tgttgtggta
     8281 tttgttatgc agtttcaccg taatcaagcc cattaccagc gagccgatca caccggtatc
     8341 aatccctttg gtttcaggag gaaacagcgg aatgagggca gaaatcgtgc ctgtcataat
     8401 caaataaccg accactgctg ccagtgccgc tacgccttta tctcgttttg ctaatccaat
     8461 gcctaaaccg atacacagta ataatgcaag gttgccaaac accacactgc ctgccgatgc
     8521 cataatctgg aaaatgccct gtaaagtcgg gttatccagt atcgggtagg cttgcaccgt
     8581 tgccttattc gacaaagctc cgccaatccc taacagcaaa ccggctgccg gtaaaatcgc
     8641 aatcggcagc ataaaagctt ttccgacttg ttgcagttgt ttgaacataa aatttctcct
     8701 tttttatggt tgtatttgat ggtttatagc atactgaaat ttagccgtat gaggtgtgat
     8761 ataactcaca gatttgcaaa atttttagca aattcgaccg cttgtaaaaa aattaccgct
     8821 tgcggaaaaa ttgaagtaga attaagcatt tttttagcaa acacaacata aaaaatttgg
     8881 agaagattat gcctatttta cgttcagcaa cttccactca gggtcgtaat atggcggggg
     8941 ctcgtgcatt atggcgtgca accggtatga aagaaaatga ttttggtaag ccgattattg
     9001 cagtggtgaa ctcattcacc caatttgtgc cggggcacgt ccacttaaaa gatatggggc
     9061 agttggttgc ggcagaaatt gaaaaagccg gtggggtggc aaaagaattc aacaccattg
     9121 cggttgatga cggcattgca atgggacacg gcggaatgct ttattcttta ccaagccgtg
     9181 atttaattgc agacagtgtg gaatatatgg tgaatgctca ctgtgctgat gcgatggtgt
     9241 gtatctctaa ctgcgataaa atcaccccgg gaatgttaat ggcagcaatg cggttaaata
     9301 ttccgactat ttttgtctct ggtggcccga tggaagccgg aaaaactaaa ctttccgatc
     9361 aattaattcg cttggattta gtcgatgcaa tgatcgaaag tgccgacccg aatgtgtctg
     9421 atgaacgtat tgatgcgatt gaacgtagtg cttgcccgac ttgtggttct tgttcaggta
     9481 tgttcaccgc aaactcaatg aactgtttaa ctgaagcttt aggcttaagt ttaccgggca
     9541 atggttcaat gttggcaact catgctgacc gtaaagagct tttcctaaga gcaggtcgtg
     9601 aaattgtgga actttgccgt aaatattacc aacaagacga tgagagcgtg ttacctcgct
     9661 caatcgctac cttcgatgca tttgaaaatg caatgagcct tgatattgca atgggtggtt
     9721 caagtaatac cgtgcttcat ttactagcga ctgctcaaga agccggcgtt gattttaaaa
     9781 tggaagatat tgaccgcctc tctcgtgtcg tgccgtgctt aagtaaaatt gccccgaata
     9841 ccaataaata ccatatggaa gacgtacacc gtgccggtgg gattatgggc ttgctgggcg
     9901 aattagatcg tgcaggttta atccatcgcc acgttaaaac agtgctaggt ttaacgatag
     9961 aagagcaact gaatcaatac gatattattc gtaataaaga tgaagattta cacaaattct
    10021 tccgtgcagg tccggcagga attcgtacta ctaaggcttt ctcgcaagat tgccgttggg
    10081 acactgttga tgatgaccgt gtaaacggct gtattcgcaa tgtggaaaat gctgtgacca
    10141 aagagggcgg gttagcggta ttatttggga atattgcaga agatggatgt attgttaaaa
    10201 ccgcaggggt agatgagtct atttggaaat tcactggcac cgcaatcgtg tttgaaagcc
    10261 aagaagatgc ggtagcaggt attttaggcg gaaaagtgaa agaagggcat attgtgatta
    10321 tccgctatga aggtccgaaa ggcggcccgg gtatgcaaga aatgctttat ccaactagtt
    10381 atttgaaatc aatgggctta ggcaaaaaat gtgctttatt aactgacggt cgtttctctg
    10441 gcggtacctc agggttatct atcggacacg cttcaccgga agccgcttca ggaggagcga
    10501 ttgccttaat taatgatggc gatacggtgg cgattgatat tccgaatcgt tcgattaact
    10561 taatggtttc agatgaagaa cttgctgctc gccgtgcaga aatggaagcc cgtggcgata
    10621 aagcgtggca gccgctaaaa cgtgagcgag aagtgtcgtt tgcactaaaa gtgtttggac
    10681 attttgccac ctctgctgat aaaggtgcgg tgagggataa atcgctgtta aagtaaataa
    10741 attagaaaaa agaagcattt caataatata ttgatctgct ctttattcag ctgaatattt
    10801 aatctagctg aatggagagc agatcgatag cctaatttac atctttttgt tttgatacag
    10861 ggaaatgtat tacaagcatt ttgccggttt tggcagacct gcaattttag tggcttgttt
    10921 tgcaggacca tcaggaaaca agcgttgtaa ataacggctg ttgccttttt cttcaccgaa
    10981 tttttgtgcc attgtcttta ccagcattcg aattgctggt gaagttttgt attcttggta
    11041 gaaatcccgc accagataaa taatttccca atgttctgcg gttaagctaa tttgctcttt
    11101 ttcggcgatt ttttctgcta attctacaga ccaatcattt aaattagtta aatagcctga
    11161 agcatcggtt gggtattgga tattatttaa ttcaatcgtc atcaaataaa aatgccacat
    11221 aatgtggcat tcctcttaaa ctaaaacgct agtcgtcact acctagcaca ccgaagattt
    11281 gtaagaagct tacaaatagg ttgtagatag acacatataa atcaacggtt gcacgaatgt
    11341 agttggtttc gccaccgtga ataatgttgc tggttgccag taaaatcgca ccgcttgaga
    11401 aaatgacaaa caagctgctt agggctaaac ttaatgccgg aattgctaag aagatattgg
    11461 caataatacc gactaataat accacaaaca gggcaaccat cattccgctg ataaagctca
    11521 tatctttttt ggtggttaat acataagctg aacaagcgaa gaaaaccaat gcggttgcac
    11581 cgaatgcaag ggcgacaata tcccctaaac ccgcaccgat gtaacggttt aaaataggac
    11641 ctaaggtata gcctaagaag ccggttaaag caaatacgct taagataccc gcactgctgt
    11701 ttgaggtggc attcgtcaag aaaagtaagc cgtaaaagcc gaccaataat ccccaccacg
    11761 gcatagccgg tgcgttcatt tgaattgcaa caaaagcaac aactgcggag aaagtcaggg
    11821 tcattgccaa taacatatag gtattgcgaa gcactttgtg ggtgctaatt agagattcac
    11881 cagttgaata gctgtgagct aaacgatttt ccataaaaat tccttctgtt tttgtgttta
    11941 aatcggaaca tccgatggat taatatctat ggataaattt ccataaatca agaagtaagg
    12001 tagccgtaat ttattgtaaa gtcaatgcga ttttgtaaca gaagagtaaa aaaaaacatt
    12061 tagttgtgtt acacttaata ccactctact ttatattcct tttcatcgat gaattcgcta
    12121 tgaagctcaa tttcccaact tatttaacct tatttcgggt aggattaacc ccacttttta
    12181 ttgtggcttt ttatctgccg acaaaatatg cgcctgaaat ttcaacactt atttttttta
    12241 ttgcatcgat taccgatgcc tttgacggct atttagcacg caaatggaat caaaccaccc
    12301 gacttggtgc atttttagat cctgttgcgg ataaagtctt ggtagctgtt gcgtttgtct
    12361 gcgtggcgga gtattaccat acttggtgga ttactattcc aatctgcatt atgattgccc
    12421 gtgaaattat tatttcggct ttgcgtgaat ggatggcgga gcttggggaa agggccagtg
    12481 ttgccgtttc gatttggggg aaagtaaaaa cgactgctca aatgctcgca ttaggtggaa
    12541 tgttatggag acagaaccaa ctaatggaga ttttagcctt tattctgctt tatattgcag
    12601 tgggtttaac gatctggtct atgatgcagt atttaaatgc ttcaaagaat agtctattga
    12661 agtcataagc ggtcatattt ttattctttt ttgcaatttg tagagtggaa atatcgctct
    12721 acaattttca attttatcaa ctctaatgtc tgaatttaac gctaaatcct tcctgttaaa
    12781 tgtgcctcac caaccgggtg tttatcgtat gtatgatagt aaaggcacga taatctatgt
    12841 gggtaaagcc aaagatttaa aaaagcgttt agccagctat tttcgtagcc aactttccag
    12901 taaaaaaacg gaagccttgg tggcaaatct tgctaatatt gaaacgacga tcactcattc
    12961 tgaaacagag gcgttactcc ttgaacataa ttatattaag gaaaatcagc cgaaatataa
    13021 tgtgttgctc agagatgaca aatcttatcc ttatattctg ttaactaaac ataagcatcc
    13081 tcgtttaaca gcatttcgtg gtagtaaaaa gatcgccggt gaatattttg ggccttatcc
    13141 gaatgttacg gcagttcggg aaacactgaa tttattgcaa aaaatttttc ctattcgtca
    13201 gtgtgaagat acttattata aaaaccgttc acgaccttgc ttacagtatc agatcggacg
    13261 ctgtttagcc ccttgtgtag agggctgtta ttcacccgtt gaatacgatc agcaggttaa
    13321 tttggttcgt ttgtttttac agggaaaaga tcaccaagta gtcgagcatc tggtacagaa
    13381 aatggaagat gcggcggagg ctttggattt tgaaggtgcg gcacgttttc gggatcaaat
    13441 tcaatcagta agagcggtgc aagaaaaaca attcgtctct aatgataggc tagatgatct
    13501 tgatattatc tcgatcgttt atcaaaatgg gattgcctgt gtgcatatct tatttgttag
    13561 gcaaggtaag gtactaggca atcgttctta ctttccaaaa gtgccaagta atacagattt
    13621 aaccgagctc gcagatacct ttgtcggtca gttttattta caaatgaacc agcaccgcac
    13681 catacctaac caaattgtaa ttgatcatca attaagtgaa tctaaggcac tcagtgaaat
    13741 tttaagtgag caagcagggc ataaagtcag cattttagat aatgccatca gaggtgataa
    13801 acagcgttat ttagctttag cgagaactaa tgcaatggca gcattagttt tacaacttaa
    13861 gcaagaatcc catattcaaa gccgttatca ggcattgaaa gaaatgttga aaatgtcaga
    13921 gattaaaaga atggagtgct ttgacatcag tcatacaatg ggcaaccaaa cagtggcttc
    13981 ttgcgtggtg tttgatgaaa atgggccgct aaaatcggat taccgccgtt ttaacattga
    14041 ggggattacc ggaggtgatg attatgcagc aatggagcag gctctcttga aacgctataa
    14101 tcgagaatta ccgccagaaa agatccctga tattattttt attgatggtg gcaaagggca
    14161 gttgaaccga gcgttagatg tcttcgctaa acttgatgtg gaatgggata aatcgaaacc
    14221 gcttttaatt ggtgtggcaa aaggcgtaga acgtaaagct gggctggaaa ctttaattat
    14281 cagcaaatgg gataaagaaa ttcacttacc gccagatagt cttgccttgc atttaattca
    14341 gcatattcga gatgaatccc accagcatgc cattagtggg catcgtaaaa aacgccaaaa
    14401 ggcctttacc gaaagtggat tagaatctat tgccggtgtc ggtgcaaaac gccgacaagc
    14461 gttgctaaaa tatttaggcg gaatgcaggg ggtgaagtct gcaaccttgg aagagattca
    14521 atcagttccg ggaatttcta aagcgttggc agaggttatt tttgatacat taaaaaatag
    14581 ttaaattatc aatagcgata tcgcttccag ccgtaatggt agcccactcc tatgcttggg
    14641 taaattatag ggtgaattgg cagatttatg ctttctgagc gtttatcgct ttggtttaat
    14701 ggctcatcat gttgctttgt atttttatca acagtttgct tattgattac tcgttgatgg
    14761 taatcctgaa ctgcttgcat atccattggt actgtgcttt gttgttgggg tgtcgaacag
    14821 gcggttagtg caacgctcaa tccgagtaaa attaattttt tcatatcatt tccttatatt
    14881 gcaaaatatt ttgctttttt gaccgcttat tttgctatta attcttaaaa agattatttt
    14941 atccataaaa aaggacgcac taggcgtcct catttctatt ctttaaaatt aaagagcaga
    15001 ttttgctttt tcaactaaag ctgcgaaagc aactttgtca aatactgcga tgtcagctaa
    15061 gatcttacgg tcgatttcaa cagatgcttt tttcaagccg ttgatgaatt tgctgtaaga
    15121 taaaccgttt tgacgagctg ctgcgttaat acgagcaatc cataattgac ggaattgacg
    15181 tttacgttga cggcggtcac ggtaagcata ttgagcagct ttaactactg cttggaaagc
    15241 aacgcgatac acgcgtgaac gtgcaccata ataaccttta gcagccttaa gaactttctt
    15301 atggcgtgct cttgcaataa caccacgttt tacacgagcc attatttaat ctcctaattg
    15361 tctattttga ttggtggtcg tcatcacgac ctttactaag aattctatcc gtacgtccgt
    15421 actttactac actacgctaa cctaaaaggc ttatgcgtat ggtaagcacg ctactactaa
    15481 aacttggtca gctttcgcta ccattgattt gtgacgtaat tgacgtttac gtttagtggt
    15541 tttcttagtc aaaatgtgac gtaagtgaga ttgtttacgt ttaaaaccgc cggaagctgt
    15601 ttttttgaaa cgcttagcag caccacgtac tgttttaatt ttaggcattg ttaaaaaact
    15661 ccgcattgtt tgttagatat tatgaaaata aggcgaattt tgtgaacaaa attacttgga
    15721 agcacttatt ctccgctata cgattttcat cgttaaaaag tcaagcaggc tgctgagttt
    15781 gcctgttgag cattttctct ttcttggcta ggcaaaaaag cttgcgaatt ttagcgaaaa
    15841 tataaccgct atgcaagcca atttaagctt tatcaagaaa aaagcacatt aaaacaatgt
    15901 gcttatcgaa attatttctt cttaggtgcg atcaccatca caacttggcg accttccaat
    15961 ttgcctgggg ctgactctac cattgcaatt tctgcagtat ctgctttgac acgttctaaa
    16021 acgtccatac cgatttcttg gtgagccatt tcacgaccac ggaaacgcac tgtgattttt
    16081 actttatccc catcttctaa gaaacggata atgctacgaa gtttaacttg ataatcgcca
    16141 tcatcagttc ccggacggaa tttgatctct ttaacttgaa caactttttg ttttttcttt
    16201 tgttcttttg ctgctttctc tttttcgtag atgaacttac catagttcat aatacgacaa
    16261 actggtggtt cagcatttgg gctgatttcc actaaatcca attcagcttc ttctgctcta
    16321 tttagagcat cttgaagtga tacaatacca acttgttcgc cgttttcatc ggttaagcga
    16381 acttctttta cgccacgaat ttcgtcatta agacgatgtg cacgatttga ttgaacacgt
    16441 tttacaggtt taatagcctt attccttcta gtaaattttt gcttaaattt taccgcttga
    16501 gagcgtataa atattttaga ttgcttgttt taagtgatta aaaaacagct aaacgtgcgg
    16561 attctataag attttcagtg cttatgcaat aataaagccc ttatttaact gcctattttc
    16621 ggttattttg atattctttg attaaactca taaatgcttg cccaaatcgt tcaaattttc
    16681 gttcaccaac accattaatt tctaacattt ccacttttga agtcgggaga aattctgcca
    16741 tttcttgcaa ggtggcatcg ttaaagacga tataaggtgg aatattttcg aggtcagcta
    16801 tctgcttgcg taaaaaacgt aagcgtgcga ataggtcttt gtcatagcga tgacttgctt
    16861 gtttttgcac aaatgcagtc gcagatatac ctaaacgtgg caatgcaagc tgaagttgct
    16921 tttctccacg caaaaatggt ctggcacttt cggttaattg cagagcggaa tggttagcaa
    16981 tattttgaat aatcaaccct aaatgaatca gttgccgcac cacattttgc caataatgtt
    17041 ggctttgttc ttttccgata ccataaacgg aaagttgatc gtgttgatgt tcacgaattt
    17101 tgagattgtt cattcctcgc aataccgcaa taatatggtg ctgaccgaaa atttgaccgg
    17161 tgcggtaaat caccgacatc actttttggg catcaagtaa gccatcgtat tgttgcggtg
    17221 gatctaaaca aatatcgcag tttttgcatt gctcttggcg atgttcccca aagtagttga
    17281 gtaagactaa acggcggcaa gtctgtgatt cggcgaaatc gccaattgcc tgtaatttat
    17341 gctgtttaat atttctctgt tcgctttccg gctcttccaa taatattttt tgtaaccaag
    17401 cataatcgct aggatcataa aaaagcacgg cttcagaagg gagatcatct cgcccggctc
    17461 gtccggtttc ttgatagtaa gattcgatag agcgaggtaa atcgaaatgt accacaaaac
    17521 gtacgttgga tttgttgatc cccataccaa acgcaatagt ggcaaccaca atttgaatat
    17581 catcacgctg aaaggcattc tgcactcgtt ctctctgttc aaaactcata ccggcgtgat
    17641 agcccatcac gctgatttta cgggctgaga gtttctcggt gatttcttcc actttgttgc
    17701 gactgttgca gtaaacaata ccgcttttgc cttgctgttt gctgatgaat ttaatgagct
    17761 gttcaagcgg tttaaatttt tcctgcacgg tgtagcgaat attggggcga tcaaagctgc
    17821 ctaaatagat atgtgggcta tttaagcgta aatgatgcag aatatcggag cgggttgtta
    17881 gatctgccgt ggcggtgagt gccatcaacg gcacattcgg gaaagtgttt cgtaagccac
    17941 ctagcagggt atattcaggg cggaaatcgt gcccccattg ggaaacacaa tgggcttcat
    18001 caaccgcaat aaagctgatt ttacatagcg aaataaagtg gaaaaagcct tgagtcatta
    18061 ccttttcagg ggaaagatag agtaatttta attgaccgga taaggctttt tgttccacaa
    18121 gttgctgctc ttcgagcgtt tgagtggagt taagataagc tgcttcaatg ccgttggtga
    18181 taagttgatc cacctggtct ttcatcaacg aaataagcgg cgaaatcacc aaagtgatgc
    18241 cgtctaggca caaagccggc acttggtagc aaagggattt gccaccgcct gttgtcataa
    18301 tcacgaggca atcacgattt tccaacacgg cattgataac ttcttcttgc ccgtctcgga
    18361 atttttgata gccaaaaata ttatttaaaa cagaaattgc ggtggtgttc attggtagtt
    18421 ggttgctact tatgatagta ctcgtcttct gagagtatct tttaaaatga aaataaagca
    18481 ctcatcaaag acgagtgctg tcttagttaa caattaggca ttagaaagaa acgctttctc
    18541 cgtgtcgagc aaaagcttct tctaaatgtt gccagaacga gcgaccttca tctgttaccc
    18601 attcgccttc tacattttta aagtggaaac cacccaattt gctggcaagc cataattcca
    18661 gcattgcctc ttgtttatta atgataactt gcgattcatc atcaaaggtt aaggtgcaga
    18721 ccgctccttg gatttcagta tcgactgata atccttcgtc ataaattttt tcttcaattt
    18781 gttgccatac ttgctctacg gcttggtgga attgtgctgt gttcattggt gtatccttat
    18841 ttgaatgtta tataagtagt aaatactttt tgttttagct
//
LOCUS       DANLUA010000025        18838 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLUA010000025 DANLUA010000000
VERSION     DANLUA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            29..979
                     /gene="tal"
                     /locus_tag="PXB44_000677"
     CDS             29..979
                     /gene="tal"
                     /locus_tag="PXB44_000677"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL5281188.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(1041..1314)
                     /locus_tag="PXB44_000678"
                     /pseudo
     CDS             complement(1041..1314)
                     /locus_tag="PXB44_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(1345..1809)
                     /locus_tag="PXB44_000679"
     CDS             complement(1345..1809)
                     /locus_tag="PXB44_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5281189.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(1830..2429)
                     /locus_tag="PXB44_000680"
     CDS             complement(1830..2429)
                     /locus_tag="PXB44_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5281190.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            2463..4190
                     /gene="argS"
                     /locus_tag="PXB44_000681"
     CDS             2463..4190
                     /gene="argS"
                     /locus_tag="PXB44_000681"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL5281191.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SGDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            4380..5546
                     /gene="cgtA"
                     /locus_tag="PXB44_000682"
     CDS             4380..5546
                     /gene="cgtA"
                     /locus_tag="PXB44_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL5281192.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            5631..6248
                     /locus_tag="PXB44_000683"
     CDS             5631..6248
                     /locus_tag="PXB44_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL5281193.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(6295..8253)
                     /locus_tag="PXB44_000684"
     CDS             complement(6295..8253)
                     /locus_tag="PXB44_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL5281194.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(8250..8807)
                     /gene="ccmE"
                     /locus_tag="PXB44_000685"
     CDS             complement(8250..8807)
                     /gene="ccmE"
                     /locus_tag="PXB44_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL5281195.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(8993..9190)
                     /gene="ccmD"
                     /locus_tag="PXB44_000686"
     CDS             complement(8993..9190)
                     /gene="ccmD"
                     /locus_tag="PXB44_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL5281196.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(9205..9942)
                     /locus_tag="PXB44_000687"
     CDS             complement(9205..9942)
                     /locus_tag="PXB44_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL5281197.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(10142..10804)
                     /gene="ccmB"
                     /locus_tag="PXB44_000688"
     CDS             complement(10142..10804)
                     /gene="ccmB"
                     /locus_tag="PXB44_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL5281198.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(10801..11442)
                     /gene="ccmA"
                     /locus_tag="PXB44_000689"
     CDS             complement(10801..11442)
                     /gene="ccmA"
                     /locus_tag="PXB44_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL5281199.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(11586..12296)
                     /gene="hda"
                     /locus_tag="PXB44_000690"
     CDS             complement(11586..12296)
                     /gene="hda"
                     /locus_tag="PXB44_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL5281200.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            12566..14167
                     /locus_tag="PXB44_000691"
     CDS             12566..14167
                     /locus_tag="PXB44_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5281201.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            14439..15443
                     /locus_tag="PXB44_000692"
     CDS             14439..15443
                     /locus_tag="PXB44_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5281202.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            15545..16438
                     /locus_tag="PXB44_000693"
     CDS             15545..16438
                     /locus_tag="PXB44_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5281203.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            16449..17438
                     /gene="dppD"
                     /locus_tag="PXB44_000694"
     CDS             16449..17438
                     /gene="dppD"
                     /locus_tag="PXB44_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL5281204.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            17566..18555
                     /locus_tag="PXB44_000695"
     CDS             17566..18555
                     /locus_tag="PXB44_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5281205.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
BASE COUNT         5675 a         4141 c         3940 g         5082 t
ORIGIN      
        1 atttcacaat caaaaaagga atcagactat gagccaactt gacaccttaa gcaaaatgac
       61 cgttatcgtt gctgacactg gtgatattga agcaatcaaa ctttataagc caaaagatgc
      121 aacaacaaac ccgtctttaa ttttaagtgc atcggcatta ccgcaatatg ctccattaat
      181 tgaagaagcg attaattacg caaaagcaca aagtagcgat aaagatcaac agttagttga
      241 tgctgaagat aaattagcgg taaatattgg tttagaaatc ttaaaacttg tacctggacg
      301 tatttctaca gaagttgatg ctcgtttttc ttatgatact gaaaaaacta tcgctaaagc
      361 acgtaaatta attggattat ataacgatgc aggcatcagt aatgatcgca ttttaattaa
      421 aattgcctct acttggcaag gtataaaggc agcagaacag ttagaaaaag aaggcattaa
      481 ctgtaactta acgttattat tctctcaagc tcaagctcgt gcgtgtgcag aagcaggggt
      541 ttacttaatc tctccattcg ttggtcgtat tttagactgg tacaaagctc acgaaggtaa
      601 agaatatgct ccagctgaag atccaggtgt aatttcagta accaatatat acaattacta
      661 caaacaacac ggttatgaca ctattgtaat gggtgcaagt ttccgtaatg taggagagat
      721 cattgagtta gctggttgcg accgtttaac catctcacca ccgttattaa aagagctaca
      781 agagagcgat gaaaaattag tttgcaagct agattacaaa ggtgaaatca agcctcgtcc
      841 agaaaaaatg acagaagcag aattctactg ggagcataac caagagccaa tggcagtaga
      901 aaaattagct gaaggcattc gtaaatttgc gattgaccaa gagaagttag aagcaatgct
      961 tgctgaaaaa cttcaataga caacgcataa aaaaaccggc attaacgata attaatgccg
     1021 gttttaattt ctataaatga ttatttaaac tcataacgtc taatcgtttt tagatcgcta
     1081 tcttcatttt taatttcatc ggcaagatga acacgagcac caaccgctgc atgtgcatca
     1141 tactcacgag ttagagtcac agtttcacca ttatctcgtt gaactgtaag ttcaacaaca
     1201 tcgtttgata ctgctttgct agataaatgt tgcattacct ttatcagaat aaacatctgc
     1261 agtacaagct gctaacataa gtgaagctaa tacagctaaa gaaagttttt tcattaataa
     1321 tatatcctct aggaaaaact aaaattaaag cagagaaaca ttcaggtctg aattagaccc
     1381 tacgattctc acacgtttgc caggcacaaa accgtcttct ttcttttgaa cgaccacgat
     1441 ttctttacca tcatctttgc gaataaccat ttccaatgaa gaaacttgac tcgctttttg
     1501 ttctacttga ttaccaatta ccgcaccggc aactgccccc actgcggttg caatcgcttg
     1561 ccctgtacca ccgccaatcg cattaccggc aacgccacct aatacaccac cgccgacagt
     1621 accgatcaca ccttcactgc gtgcttgaat tttcacatca cgcactgaaa caatagtgcc
     1681 ataagcgata gaacgagctt cttttgcctg ctcaccggaa taaacgcttc cgctaaacac
     1741 gtctgtattt gcacagccaa ctaaagcaaa acttgacatt aatgcgacag ctaaagctaa
     1801 ttttttcatt ttgtttgttc tccgaaacgt taattttctg acataattac actttttata
     1861 tcataagggt ggagctttag acaataagga ttacaaaaag ttacatttct caagctgatc
     1921 gcaatgcttg gatttggcaa tctctcccct tctacctgag gttggctgat ttttaattta
     1981 atctcagaat tatcagcaag tctaaagtgc gacaatgccc ttgacaccca ttgttccgaa
     2041 ctttcgttgt ctaacatcgg aaaaaccagc tcaatatgtt cccagccttc ttctaaataa
     2101 accttatttt tcggaaaagg caactcgaca atctttacaa attgtccgca aaattcaacc
     2161 gcttgtatta atgcaaacag ccctatcggt ctgccgttta cctcgctctc tttaagtaag
     2221 gttgccccat caagcaaaag gatcttccac tgttctgcgg tttcggtttg gttcattctt
     2281 accgcaagat gatcgatttc atacgctgct aaatcaatgc cggcgatttc agcaatttgt
     2341 cggattttct gttcaaattg cgataaatcg ccaaaacaag cggtcatttt ctcgaaaaat
     2401 tttgcatttt ctgctataat tttgtccatt tttttgtaaa tagaatttaa ataggttaaa
     2461 cagtgaatat tcaacaaatt ttatcagata aaattaaaca ggcaatggta gcaagcggtg
     2521 cagacagcaa tgtcgagcct ttagtccgcc aatcgggcaa gccggaattt ggcgattacc
     2581 aagccaatgg tgcgatgggt gcagcgaaaa aattggggat gaatccgcgt gaatttgccc
     2641 aaaaaatctt agagaatgcc gatttgaacg gtattgccga taaattagaa attgccggtc
     2701 cgggctttat caacattttc ttaaacccag aatggctggc ggagaacact aacagttcat
     2761 tgcaagcggt caattttggc ataaaaactg caaatccgca gacgattgtg atcgactact
     2821 cctcgccaaa cgtagcaaaa gaaatgcacg tcgggcattt gcgttccacc attattggcg
     2881 atgcggtagt tcgtaccctt gcgtttttag gcaataatgt gattcgtgcc aaccacgttg
     2941 gcgactgggg tactcaattc ggtatgctga tcgcttacct tgaaaaaatg gaaaatgagc
     3001 acgccagcga aatggagcta agcgatttag aagccttcta tcgtgcggca aaagagcatt
     3061 atgattcaga cgaagcattc gccgaaaaag cccgtaacta tgtggtgaaa ttacaaagcg
     3121 gcgataaata ttgcttaaca atgtggaaaa aattggtcga tattacaatg caccacaatc
     3181 aagagaacta caatcgctta aatgttacgc tcaccgaaaa agatgtaatg ggcgaaagcc
     3241 tctacaatcc aatgttgcct gaaattgtgg cggatttaaa agccaaaggc ttagcggtgg
     3301 aagatgacgg tgctttagtg gttttcctcg atgaatttaa aaataaagat ggcgacccaa
     3361 tgggcgtaat cgtgcagaaa aaagatggcg gtttcttata taccactacc gatattgcgg
     3421 cagcaaaata tcgctacgag accttaaaag ctgaccgagc cttagtcttt tctgacagcc
     3481 gccaagctca acatatgcaa caggcatggc tcattacccg caaagcgggc tatgtgccgg
     3541 acagtttcag ccttgaacac ccgttcttcg gaatgatgct aggcaaagac ggcaaaccgt
     3601 tcaaaacccg tagcggtggc acagtcaaac tcaaagattt attagatgaa gcggtagagc
     3661 gtgccgataa actgattgcc gaacgttcaa ccgagctaac tgcggaagaa aaagccgccg
     3721 tggttgaagc ggttgcgatt ggttcggtga aatattctga cttatcgaaa aaccgcacca
     3781 ccgattatgt gttcgattgg gacaatatgc tgacctttga aggcaatacc gccccgtata
     3841 tgcagtatgc ctacacccgc attcgctcca tctttgcccg tgcgaacatt gacgaaaatg
     3901 cattgagcgg ttcaatccaa ctcaatgagc caaaagagcg tgcattagca gtgaaactat
     3961 tgcaatttga agaggcatta aacggcgtgg caaaagaagg tatgccacat attttatgcc
     4021 aatatttata cgagcttgca ggtagtttct ccagtttcta cgaggcttgc ccgattctca
     4081 atgccgaaga aacaaccaaa aacagccgtt tgaaactcgc tgccttaacg gcgaaaacct
     4141 tgaaacaagg gttggattta ctcggtatta aaactgtaga gaagatgtaa tacaaactct
     4201 ctccccttgt gggagagaga ccgcttatga gcgttcagcg aaataagcag agagagggga
     4261 caagcggtaa tttttattaa aatttttaca cattttcccc tctccctgat ttttcttctg
     4321 aacgaagaaa aatctgtccc tctcccacaa ggggagaggg catataaaag gaaacaaata
     4381 tgaaatttat tgacgaagcc ctgattcgtg tagaagcagg cgatggcggt aatggctgtg
     4441 tgagcttccg ccgtgaaaaa tttatcccga aaggcggacc tgacggtggt gatggtggcg
     4501 atggtggtga tgtgtattta attgccgatg aaaacctaaa cacccttatc gactaccgct
     4561 ttgaaaaacg ttatgccgct gggcgtggcg agaatggtcg cagtgccggc tgtaccggac
     4621 atcgtgggca agatattacc ctacgagtgc cggttggaac ccgtgcgatt gataacgaca
     4681 cccaagaaat tattggcgat ttaacccgcc acggaatgaa aatgctggtg gcaaaaggtg
     4741 gttatcacgg tttaggtaat acccgcttta aatcctcagt aaaccgagct ccacgccaaa
     4801 aaaccaacgg tacgcctggc gaaaaacgtg atttgaaatt agagctgtta ctgcttgccg
     4861 atgtgggaat gcttggctta ccaaacgccg gtaaatccac ctttatccgc agtgtctctg
     4921 ctgccaaacc aaaagtggcg gattacccgt tcacgacgct ggtgccaagt ttaggggtag
     4981 cccgtgtggg ggccgaccgc agttttgtgg ttgccgatat tccggggtta attgaaggtg
     5041 cagcagatgg tgcagggctg gggattcgtt tcctaaaaca cttggaacgt tgccgtattt
     5101 tgattcactt agtcgatatt atgccgattg atgaaagcga tccggctcat aatatttcgg
     5161 tgattgaatc cgagctttac caatacagcg aaaaactcgc cgaaaaaccg atctggttgg
     5221 tgtttaataa aattgacacc atcggcgaag aagaagctgc tgagcgagca aaagacattg
     5281 ccgagcaaat cggctgggaa ggcgattact acttgatttc tgccgctacc ggtcaaaacg
     5341 tgcaagctct cactcgtgat attatggact ttattgaagc caacccacgc gaagtggagg
     5401 aagaaaataa agcacctgaa gaagtcaaat tcaaatggga agactaccac aacgaagcaa
     5461 tgcaaaatgc ggtagaagaa gattgggacg acgattggag cgaagaagat gacgaaggca
     5521 tagagtttat ttacgaacgc cattagtgca ataatacaag cggttgtttt taaacggaga
     5581 tttgcaaatt tccggagaaa aacaaccgct tgtaggttta aggagagttt atgcttagct
     5641 caacatcgtg cgatttattc aatattccgt tcttccaatt tgctcagatg aagaaatatt
     5701 gccctgagga gatccctcga attaaggcgg aatataaaac tcaatgggaa atttggaaaa
     5761 agctacatca ggaagtttct cgtcagcttg ggcagccgtt tgctccacca catattgaga
     5821 agtggtgcaa cggttggcaa gttcgagccc atttttttgc ctattacaag tatgaattta
     5881 atcgaaattc tgcggctatt ctttccgtga ttttaaatcg tcgccgtttg caagtgagcc
     5941 ttgattggca ttgttatcga gccgatcgct cgcaaattaa cgtgcatcaa tacaatcaat
     6001 ggtgggaaaa tttagatcgg gaaaagtttg ctgattttga gatttggcac ggaagtgaga
     6061 gtgaatatga tgattttaga accgttagtc agatgtcaga gcaagcgttc accctaacta
     6121 acgaggaaga tttttggtgt atcggcaaaa atgtggaaaa ggacgatttg gataacattg
     6181 atgtagtaga atttatctac caaaccattc gggcattatt gccactttat gagaaatgtc
     6241 atcaataaac actaaaaaga gaggcgaaca tcatttgttc gcccctttca aaatttaagc
     6301 tatcacttct tttttatttt caatgcgtaa acggtagcgt ttatccaaca tacagagtaa
     6361 accaccgaga gccataaaca gcccgccaat ccaaatccag cgaatgaacg gtttataata
     6421 caaacgcact gcccaagagt tatcttctaa tttttcgcca agtgcagcgt ataaatctcg
     6481 ggtaaagccc cagtcaatcg ccgcttcggt cattcccatt cggctcaccg tatagaaacg
     6541 tttttcagca ttaagtgtgg cttcatattt accgtttcga gtaatttcaa gctcggcagt
     6601 gccgccttga tagtttgccc catcggtgat tttaatacct ttaaattcaa attggtaatc
     6661 taaaatctca gctttatcac ccacattcat tctgacatct cgttcaatgc tgaaattttg
     6721 gctaaatgca atgccgaaca ctgtcatcgc aacccctaag tgagccagca ccattcccca
     6781 atgtgaacgg gacagtttga aaatgccact taagaacgag tggcgatggg ttgcacgttg
     6841 gtgtagctca tacaggctaa gtaacacaat aaagacagac atcatcgtgc ctaacaccac
     6901 actcacggta atggtgttac ggaataagta tggcaatccg aatccaagca atgccattaa
     6961 aatagttgca ataattaccg gtttacgaat agcagaaact tggtctctac gccatttcac
     7021 taatggtcca atccctaata caaatgagaa aggaaccatt aaaattaaga acatttggtc
     7081 aaaaaatggt gccccgattg aaatcgtccc taagccgatt tgtttgtgaa ttaatggcag
     7141 taaggtccct aaaaacacca ccgataaaaa tgccattaat aaaatattat taatgagtag
     7201 catcgtttct cttgaatagc gttcatagtt atctaagctt ttaatttccg agcctttgaa
     7261 tgcatacagc agtaaagaac cacctactac taacaccaag taagccaaga tgtataaacc
     7321 acgagttgga tctgaagcaa aggcgtgaac tgacaccaaa atgcctgaac gcactaagaa
     7381 agtccccact aaacagagcg agaaagcaag aatcgctaat aagaccgtcc acgctttaaa
     7441 tgtgcctcgt ttttcagtca ctgctaatga atgaatcagt gctgttcctg cgagccacgg
     7501 cattaatgag gcattctcga caggatccca gaaccaccag ccaccccaac caagttcgta
     7561 gtatgcccac caagaaccaa gcacgatacc gagcgttaaa aatacccacg ctgccattgt
     7621 ccacggacga gaccatcttg cccacgcagt atctaaacgc cctgtcatca gtgaggcaat
     7681 cgcaaaagca aaggcaacgg agaaacccac atagcccata taaagcaacg gtgggtggaa
     7741 gatcaagccc acatcttgca gcattgggtt taattctttg ccatcaatcg gaaaatttgg
     7801 gaaagttcta tcaaacgggt tggaggtaaa aatcacaaac atcaaaaagc cgatgccaat
     7861 gacccccatt agtcctaaca ctcgagcaac ggcatcttcc ggcattttac gggtaaaggt
     7921 tgctaccccg acagaccaaa gcgacaacaa ccaaacccaa agcaataatg agccttcgtg
     7981 agcgccccac accgcagata aacgatattg caacggtaaa cggctattgg agttgttcac
     8041 cacatattgc acggtaaaat cattcaccgc aaacagataa aacagcgaac caaaagagac
     8101 agtcaatgcc aaaaacattg cccaagtcat cgggcgggca agagcaatta aggtgctatt
     8161 ttgcttttct gctccccaaa gcggtaaaac tgccaacaag atagaaatcg ctagggctaa
     8221 tgccaacgta taattgccta attctgcaat catttgcttt ccccttgcac attgttggct
     8281 ttttctgctt ccgcctcggc tttcattttg cggtcggttt ccgattcacc tttcagatct
     8341 tgttcagaca cacctgattt tttatgctgt tcttttaatt tatcgcctaa ttccggtggc
     8401 atatagtttt catcgtgttt tgccagaact tccgtagctt ttaaacgggt tggttcaatc
     8461 aacacgcctt gtgccacaat gccttgccct tcacggaata aatccggcaa aattccttcg
     8521 tattccacgg taatcgaagg cccgatatca ttgagatcaa atactacttt taaactttta
     8581 tcatcacgct ttaccgtatc ttccactacc attccgccca cacggatacg ctgaccaact
     8641 tcaggctttt gtttcggatc atcattttta ccgtttacaa tttcagacgg cgtgtaaaac
     8701 agatcgatat tttggcttaa cgcataaagt gtcaagcctg ctgcgacaga aagcccaagt
     8761 aatatcgaaa ccaccacttt taatcttgat ttgcgtcttg gattcatcgt aatcaccctt
     8821 caaaataaat gcaaaattgc ataaaaactc aaaaataaat tagtgtaaga atagcaaaaa
     8881 accgcaaaac attcccgata tttcaaggaa gttttgcgat ttctcaaatc aaatacctat
     8941 aaagaagtat gaggctatcg ttgcaaaaaa taaagttatt tcgaccgctt gtttattgtg
     9001 atgtttgctt taatttttct tcacgcacaa tctctttttt tgcttgagcc aaaatcgctt
     9061 tctgttcacg gcgactaagc caaatcagcc caatcattgc aatgaaactg acaccataag
     9121 agagccatac ataaaagccg tagccaccca ttgcaaaaaa atcgctgatt gattcaaatt
     9181 gaaatgtcat tgttctctcc tactctaaac tgattttgcc agttgtttta cccaagggcg
     9241 attacgttcg tcctttaata aggcaatgcg ataacgccaa atgctaaacc aagcggtaaa
     9301 aattaagcta ccgaaaatcg ccaatagcaa cggaattaac atttccaccg ccatagacgg
     9361 tttatcgaat ttagtgatgg tcgccccttg atgcaaggtg ttccaccatt ccaccgaaaa
     9421 gtgaataatc ggcaagttaa tcacgccaac aactgacagc acagccgccg ctttggctcc
     9481 ggtttgtctg tcttggaatg cggaataaag agccatcacg ccgatataaa ggaaaaataa
     9541 cactaaagcg gaggttaaac gagcgtccca cacccaccaa gtcccccaca ttggtttgcc
     9601 ccaaattgca ccggttgcaa gtgaaataaa agcgaatact aagccgatag gtgccattga
     9661 aatcatcgcc aagttagctt ggcgaatttg ccaaaccaat gccaccaagc ctgccaccgc
     9721 cattgagcca tatacgccca tcgaccaaat tgctgccggc acgtgaataa agataatgcg
     9781 atagctatcg ccctgttggt aatctttcgg ggcaaaagcc aagccccaaa caaaagcagc
     9841 cgctaacata atcaaactta gccaacctaa aaacggttgg attttcccta gcaaatggta
     9901 ctgcgtttcc gattttgcat aagggtgtag ccatttccac atatatttct ccaaagtaaa
     9961 taattcccct cacccgttta caggagaggg acgatttgag cttcgttcag aagcgaaaat
    10021 cagggagagg ggaaaaagtt gcaaattttc ataataattc gaccgcttcc cctctctctg
    10081 aaaaacacct ctgaacgagg tgtttttcgg tctctctccc tcaagaggga gagaggaagt
    10141 tttagttcaa actaattctt aatgcaccgg caatcgcaaa cggcgaaaaa gttaaagtaa
    10201 ccgctaaaat tgctccgata atcgccagtt gtccgctata acccatattt aaggtggcgg
    10261 cttctaatac cgcagcggca aaaatcagta ccggtaagaa taacgggagg attaataaac
    10321 tcagcaacgt accgcccttg cgtaagccca cagtgagtgc cacgccaatc gctcctaagc
    10381 agcttaaaat cggggtgccg agtaataatg tcagcactaa cgcccaccaa acgtgggttt
    10441 caagtgaaag caaaatggca gcaatcggtg aaagtaaaat caacggtaag ccggtcagca
    10501 gccagtgagc aatcactttc gctaacgcaa cctgcggcaa ggcaagcggc agtaacatta
    10561 attgctcgag cgaaccgtct aaataatcat cacggaataa ccgctcaaag gaaagcaatg
    10621 cagaaagcaa agcagctacc cacgcaatgc cgccggcaat tttgcctaat aattcggggt
    10681 taggtcccat taaaatcggg aatacggtaa tcaccattaa aaaaaaccac aacggattta
    10741 aaatctccga gggtttacga aaggcaatgg tgagttcccg ctgaataata gtcaataaaa
    10801 tcatacttta aattgatcta acgaaataat acgcactttg tcgctttcag cagcctggtg
    10861 gctggtaaaa atcactaatc tgccttgctc gcaatgctgt tcaatatgag caatcagatc
    10921 cgccacaccc tttttatcaa tagcggtaaa gggttcatct aaaatccaaa gtttggcgtt
    10981 agtgagccac aattttgcca acgccacacg gcgttgctga ccggctgaca aatgcgaaca
    11041 aggcaaatct tcacgcccga tgagcgacac tttcgtcaat gcgtgccaaa gggcatcatc
    11101 atcttgcggt aactgttgga ttttctggta gaaacgcaaa ttttcccacg gtgttaattc
    11161 cggttttacc cccgaataat gccctaaata aaatagttca gaataatagt gatcacgctg
    11221 tttttgaatt gcaatatcgt tccacagcac ctcaccttcc gcaggtaaaa aaagcccggc
    11281 taaaatacgt aacaggctgg tttttccaat gccgttatgc ccttcaatct gcacccattc
    11341 accactttgc acggtaaaac tgcacccttc aaacaaacgg gtttcgcccc gttcgcaggc
    11401 aatctgatta agtttaagct gatattgggc cgtttggttc attatttttc atctggttga
    11461 acattttagc cgaagtgtat cataagcctt tgatttaacg tagcaaataa attacttctt
    11521 tagaggtagc aagcggtcaa attttcaaaa agttttgcaa attttgataa gaatcaaacc
    11581 gcttgttaca gagagaggat ttccttcaca aaaggaatgg ttaatttacg ctgggcttgg
    11641 agagacgctt tatccaactt atccaatatt ttagagagtt gctctaaatc cgttcccgct
    11701 ttttttaaca gaaaatgagc gacctcatcg gacaattcca cccctttttg gtgagcattt
    11761 aactgtaaaa tttggcattt ttgttcatcg gttaaatcgt ttagctggta cacttcgccc
    11821 caagttaagc gggaacgtaa atccggcaat tggatattga gttggtgtgg tgggcaatcg
    11881 gcactgatta acagcaaggt tttttgccct tcgttaaaca agccttgttg ttctcgaatt
    11941 tggttaaaca ggttaaaaat agcaagttcc cattcagcat ctcctgcaat cgcttgaata
    12001 tcatctaggc aaatcacatt cagcagttcg gcattatcta acaccaacgg cgagaaataa
    12061 ttggctttag tgagcggaat ataactagaa gtttggttat ttaaaagaaa gtggttactt
    12121 actgctttca acaaatgact tttaccgcag ctcttaccgc cccaaatgta aaaaaacggc
    12181 tgttgaacat ccgtaaaatt tcgtttgagg gatccaagca acacttcgct gttttggcta
    12241 taaaaattgt cgaaagtgtc ttcatcaatt tgatgaatgg gtagaggtaa ctgcaaggct
    12301 ttccttaaat ctctaagttt gatatttttc taagaatagc ggaaaagcag gggtttgaga
    12361 agtaaaaata gtgagaaaat atccactcat ttttctaatg aagagatgac aagcggttga
    12421 atttccacat aattttgtca atctgaccga aaacccaccg ttttgtgtta aaaaaagatt
    12481 gcagttagct caaaaaattg tatattcaaa gctttctact taaaaagtag agtaaaacaa
    12541 ctattacaaa cacaacggag tgaatatgaa aaacatgact aaattatcgc taataacatt
    12601 agcgctagcc gcttcaagta gcgtctttgc tgcaccgaaa acctttgttt actgcttaga
    12661 ggcttcgcct tcagcattaa gcccagtgct atcaacagac ggtgcaagct ttgatgtgaa
    12721 cgccctcccg atttataaca aattaaccga ctttgaagac ggtacaacta atgttattcc
    12781 ctctcttgcc gaaaaatggg acatttcgga agacggcaaa acttacacct tccacctgcg
    12841 taaaggcgta aaattccacg acaataaaga atttaaacca agccgtgagt taaatgcaga
    12901 cgatgtagtg ttctccatta accgtcagtt tgatccaaac catccattcc acaaagtttc
    12961 cggcggtaac tacgaatact ttatcggtat ggatatgcaa aatattatcg ataaagtgga
    13021 aaaagtggac gatcacaccg ttaaaatcag cttaaaagtg ccaaatgcgc cgttcttagc
    13081 taacttagcg atggatttca acgccgttta ctcagcggaa tatgcagagc aatccttaaa
    13141 ggccggcaag cctgagcgta ttgataccaa cccaatcggc agcggcccgt tccagtttgt
    13201 ggattaccaa aaagatgcca ccgttcgtta caaagccttt gaaaactact ggcaaggcac
    13261 agcgaaaatt gaccgcttag tgtttgcaat tactccggat gcatcggtac gtttagccaa
    13321 gttacaaaaa ggcgagtgcc acgcaatgcc ttaccctaac ccggctgata tcgaagatct
    13381 gaaaaaagat ccaaatattg aattaatgag ccaatctgga atgaatatcg gctatttggc
    13441 attaaattca tcgattaaac cattagataa ccaaaaggta cgtcaagcat taaatcacgc
    13501 tgtaaacaaa caagcgattg tagatgcggt atatcaggga gccggtcaag tagcgaaaaa
    13561 cccaattcca ccaacaatgt ggagctataa tgaagcggtt caagactatg actacaaccc
    13621 tgaaaaagcc aaagcattgt taaaagaagc cggttttgaa aatggttttg atactgaact
    13681 ttgggcaatg ccggtatcac gtccatataa cccgaatgct cgccgtatgg ctgaacttat
    13741 ccaagaagac tggaaaaaag tgggcgtgaa tgcgaaaatc gtcagctatg aatggggcga
    13801 atatctcaaa cgcttacgcc aaggtgaagc ggcaacctca atgattggct ggacaggcga
    13861 taacggtgac ccagataact tcctcaacac cttattaagc tgctcagcgg tggaagccgg
    13921 ttcaaactac gccaaattct gccacaaacc gtttgaagaa gtcgtgcttg gtgctgccca
    13981 agaaagcgac aaagcaaaac gtaccgagct ttataaacaa gctcaagtga tcttcaaaga
    14041 gcaagctcct tggattacga ttgctcactc caccgtttac ttcccggtgc gtaaagaagt
    14101 aaaaggttat gtgatgagtc cgttcagctt acataacttc tacaaagtag atttagcgga
    14161 taaataatcc gccaacaagc ggtagaattt gcagattttt ttacaaattt gaccgcttgt
    14221 cctcccctct ttgaactacg aggggcaggg gggaaatttg gcagaagcga ttgccgaagt
    14281 gagaggattg taaaagagga aataaatttt ctgtcttatt gattattttc ttcgttaatt
    14341 actactgtca aatctcccct accccctctt tgctaaagag gggaatcaga tcgagagatc
    14401 agacaacttt gatgaaaatc caacctaaga gattttaaat gttttcattt attttaaaac
    14461 gtgtgctaat ggtgatccct acctttatag cgattacgtt aattactttt gcccttgtgc
    14521 attttatccc cggtgatcca attgagatcc gaatggggga acgtggatta tcgcctgagg
    14581 ttcatcaaca aatgatggaa caactcgggc tgaaccgacc gttgtttgaa caatacatca
    14641 gctacatcaa aggtgtattg cacggtgatc ttggtaactc atttcgtaac aacgaaccgg
    14701 tgctgaagga attttttacc ctattcccag ccacggttga gttggcattt tttgccctgc
    14761 tctggtcgtt gattttcggc attattctcg gtgtgattgc tgcggtgaaa aaagaaagtt
    14821 ggatttcaca cactgtaacc acgctttcac tgaccggcta ctcaatgcca attttctggt
    14881 ggggcttgat tttaattctt tatttctcca ccccgctcgg cttaccggct tcaggtcgtt
    14941 taccatctga atactggatt gaagccgaaa ccggttttat gctgattgat acttggaatt
    15001 cggacgaacc aggggcattt ttagcggcaa ttaaatctct gattttgccg tctatcgtac
    15061 tggggacaat ccctcttgca gttgtgaccc gaatgactcg ctcttcaatg ttggaagtgt
    15121 tgggtgaaga ctatatccgc accgccaaag ccaaaggctt aaacactacc cgaattgtga
    15181 ttgttcacgc attaagaaat gccttgattc cggtggtaac ggtcgttggc ttaattgtcg
    15241 ggcaactgct ttcaggagcc gttttaacgg aaaacatctt ctcgtggccg ggtatcggca
    15301 aatggattat tgatgcaatc aacagccgtg attaccctgt actacaaggc tcagtgctga
    15361 ttattgctac tattattatt ttagtgaacc ttacggtaga tctactctat ggcattgtca
    15421 atccgaggat tcgacatagt tagaaaaatt ccttcgattt ttcccctctt tagcaacgag
    15481 ggagttacac aagcggttca aattccgcaa ttttttacaa gattattttt agttggagtt
    15541 aatgatgtca ggaataacgg cggtaaatcc acccgcaccc aaaacaccgt tacaggagtt
    15601 ttggtactat ttttgtcaaa atcggggggc attaatagga cttaccttta ttgcaatcgt
    15661 ctttgttgcg tgcgtattcg caccttgggt tgcccctttt gatccaatcg aacaaaatcg
    15721 cagtgcgtta ttgctccctc ctgcttggct tgaagggggg aattccgctc atctgctggg
    15781 tactgacgat attgggcgtg atattctttc ccgtattatt tatggttcaa gattgtccgt
    15841 ctttatcggc ttagtcattg tgctactttc ttgcgtattc ggcgtgattt taggtctact
    15901 tgctggctac tatggtggca cactcgacat catcattatg cgttttgttg atattatgct
    15961 ggcgattcca agcctgcttt taactatcgg cgtggtcact attttaggcc cttcattgat
    16021 aaatgccgct attgcgattg cgattgtctc cattccaagt tatgttcgct tgacccgtgc
    16081 ttctgtatta agcgagaaaa accgtgatta tgttactgct tctcgtgttg ctggtgcgag
    16141 cgtgcttcgc ttaatgtttg tggtgatttt acctaactgt ttagctccgc ttatcgttca
    16201 gatgacgatg gggatctcaa acgctatttt agaacttgcc gccctcggct tcttaggcat
    16261 tggtgcacag ccgccaacac cggagcttgg cacaatgctt gccgaatctc gtggctttat
    16321 gcaatcggca aactggctgg tgaccattcc gggtctagcg attttatcgt tagtattagc
    16381 tttcaactta atgggtgacg gcttgcgtga cgccctcgat ccaaaactga aacaatagga
    16441 gatctaaaat ggcattatta acggttgatc aactttccgt ccattttggt gaaactaaaa
    16501 caccatttaa agcggtggat cgtatcagtt acgaagtcaa tgaaggcgaa gtattaggca
    16561 ttgtgggcga atccggttca ggaaaatcgg taagttctct cgctattatg ggcttaatcg
    16621 atttccccgg cagggttatg gctgaaaaat tgtattttaa cggcaatgat ttattggcat
    16681 taaaaccaaa agaaaaacaa cagattgtag gggctgatgt ctcaatgatc ttccaagatg
    16741 caatgaccag cttaaatccg agctatactg tgggctatca gattatggaa gccctaaaag
    16801 tgcatcaagg tggctcaaaa gccagccgtc gtgagcgagc gattgagttg ctgacgatgg
    16861 tcggtattcc cgatcctgaa tcccgtttag aagtttatcc gcaccagctt tcgggtggta
    16921 tgagccagag agtgatgatt gcgatggcga ttgcctgtcg cccgaaatta ttgattgccg
    16981 atgagccgac tactgcgtta gatgtgacga ttcaagctca aatcattgat ttattacttg
    17041 aattacaacg caaagaaaat atggcgttga ttttaatcac gcacgattta gcgttagttg
    17101 ccgagtctgc ccatcgcatt atcgtgatgt atgcagggca agtcgtggaa gagggcaaag
    17161 ccgagcagat attcaaagca ccactgcacc cttacaccca agcattgctc aaagccttac
    17221 cggaatttgc cgaagggaaa tcccgcctgc aatcactacc gggcgtagtg ccgggcaaat
    17281 atgaccgccc acaaggttgc ttactcaacc ctcgctgccc gtatgcaacc gaaaaatgcc
    17341 gaaccattga accggcaatg cagataatca acggcagaca ggtgaaatgc cacacgccac
    17401 tgaatgcaca gggtgagccg ataaggatag ctgtataacg tcataaagag tagggacgca
    17461 atgcttcgcg tccgttacaa gcggtgattt ttaaccgatt tttgcaaaat aacgatagtg
    17521 gacgcaaagc attgcgtccc tacattttcc gagaaggaaa acagaatgaa agaaaataat
    17581 tctctcccac cattcctcaa agcagtggac ttaaaaaaat actacccagt taaaaaggga
    17641 atgttcgcta aaccgaaaac ggtaaaagcg ctggatggtg tctcttttac ccttgaacgt
    17701 ggcaaaaccc ttgcgattgt tggcgaatcc ggctgtggaa aatcaacttt ggggcgaatg
    17761 ctcactatga ttgaaagccc aaccgaaggt gaactgttct acaacgggca gaatttctta
    17821 gtcaatgaca aagaaacggc tcaactacgc cgtaaaaaaa tccaaattgt gtttcagaac
    17881 ccgtatagct cgctcaatcc acgcaaaaaa gtaggtagca ttttagaaga accattattg
    17941 attaacacca acttatccgc aaaagaacgc aaagaaaaag tgttggaaat gatggcgaaa
    18001 gtcggcttaa aaccggaatt ttacgaccgt tatccgcata tgttttcggg cggacaacgc
    18061 cagcgaattg cgattgctcg gggcttaatg ttgcagcctg atattgtggt tgccgatgaa
    18121 cccgtttccg cattagatgt ttcggttcgg gcgcaagtgt tgaatttgat gatggatttg
    18181 caagaagaaa tgggcttgtc ttacgttttc atctcgcacg atttatccgt agtggaacat
    18241 attgccgatg aagtgatggt gatgtattta gggcgttgcg tagaacaagg ggataccaaa
    18301 actatcttca gcaatccacg tcacccttat acacaagcgt tactctccgc aacccctcgt
    18361 ttgaacccgg agcaacgccg tgagcggatt aaactgactg gcgaactacc aagcccgctt
    18421 aatccaccaa aaggctgtgc ctttaacgcc cgttgcagat ttgcaaccga tcgttgctat
    18481 gtggagcgac cagagctgaa aacctatcaa gacggtactc gcattgcttg ttttgtggtg
    18541 gaggaaaata attaggcaac gctcgctatt taaaaggtta aagtttttca actttaacct
    18601 ttatttttca tacttaatac tcctcatctt ttgtagcaaa atctttcatt aatttgctga
    18661 tatgctcaca atggtgttta aatgacaata cgggcaaata atcaaatgcg ttttaacgcc
    18721 aatcttctct tttaaagtga gttttcgttc ctttgagtca gaaattaacc ttgttacttg
    18781 tcggcaattc atctatttct ctccgttaat ttccttgaaa gacattgttg taactgca
//
LOCUS       DANLUA010000026        18126 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLUA010000026 DANLUA010000000
VERSION     DANLUA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18126
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(100..1431)
                     /gene="pcnB"
                     /locus_tag="PXB44_000696"
     CDS             complement(100..1431)
                     /gene="pcnB"
                     /locus_tag="PXB44_000696"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL5281206.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(1676..2122)
                     /gene="dksA"
                     /locus_tag="PXB44_000697"
     CDS             complement(1676..2122)
                     /gene="dksA"
                     /locus_tag="PXB44_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL5281207.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(2372..3325)
                     /gene="thiB"
                     /locus_tag="PXB44_000698"
     CDS             complement(2372..3325)
                     /gene="thiB"
                     /locus_tag="PXB44_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL5281208.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(3369..3512)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3796..4782
                     /locus_tag="PXB44_000699"
     CDS             3796..4782
                     /locus_tag="PXB44_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL5281209.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(4836..5255)
                     /locus_tag="PXB44_000700"
     CDS             complement(4836..5255)
                     /locus_tag="PXB44_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL5281210.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(5333..6088)
                     /gene="kdsB"
                     /locus_tag="PXB44_000701"
     CDS             complement(5333..6088)
                     /gene="kdsB"
                     /locus_tag="PXB44_000701"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL5281211.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(6098..7051)
                     /gene="trxB"
                     /locus_tag="PXB44_000702"
     CDS             complement(6098..7051)
                     /gene="trxB"
                     /locus_tag="PXB44_000702"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL5281212.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            7218..8288
                     /gene="dhaK"
                     /locus_tag="PXB44_000703"
     CDS             7218..8288
                     /gene="dhaK"
                     /locus_tag="PXB44_000703"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL5281213.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            8309..8935
                     /gene="dhaL"
                     /locus_tag="PXB44_000704"
     CDS             8309..8935
                     /gene="dhaL"
                     /locus_tag="PXB44_000704"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL5281214.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            8945..9352
                     /gene="dhaM"
                     /locus_tag="PXB44_000705"
     CDS             8945..9352
                     /gene="dhaM"
                     /locus_tag="PXB44_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL5281215.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(9392..10237)
                     /locus_tag="PXB44_000706"
     CDS             complement(9392..10237)
                     /locus_tag="PXB44_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL5281216.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(10281..10607)
                     /gene="glpE"
                     /locus_tag="PXB44_000707"
     CDS             complement(10281..10607)
                     /gene="glpE"
                     /locus_tag="PXB44_000707"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL5281217.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            10758..11216
                     /gene="exbB"
                     /locus_tag="PXB44_000708"
     CDS             10758..11216
                     /gene="exbB"
                     /locus_tag="PXB44_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL5281218.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            11253..11690
                     /gene="exbD"
                     /locus_tag="PXB44_000709"
     CDS             11253..11690
                     /gene="exbD"
                     /locus_tag="PXB44_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL5281219.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            11706..12617
                     /locus_tag="PXB44_000710"
     CDS             11706..12617
                     /locus_tag="PXB44_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL5281220.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(12686..13567)
                     /gene="cysZ"
                     /locus_tag="PXB44_000711"
     CDS             complement(12686..13567)
                     /gene="cysZ"
                     /locus_tag="PXB44_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL5281221.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            13679..14581
                     /gene="zipA"
                     /locus_tag="PXB44_000712"
     CDS             13679..14581
                     /gene="zipA"
                     /locus_tag="PXB44_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL5281222.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            14646..16703
                     /gene="ligA"
                     /locus_tag="PXB44_000713"
     CDS             14646..16703
                     /gene="ligA"
                     /locus_tag="PXB44_000713"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL5281223.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(16766..18034)
                     /locus_tag="PXB44_000714"
     CDS             complement(16766..18034)
                     /locus_tag="PXB44_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5281224.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         5530 a         3939 c         3613 g         5044 t
ORIGIN      
        1 ttttattaaa ttttttacac atttcccctc tccctgattt ttcttctgaa cgaagaaaaa
       61 tcgctccctc tcccacaagg ggagagggaa atatattaat tatccattta ctttcggttt
      121 tttcttaaag gttctacggc ggcgtgtttt ctttttctcg ctaggcacat tgcctaaatg
      181 acgaaccgat ttcagcaatt cagtacgctg accttcattg ctaaattggt attcgtgcca
      241 ccaagcggaa agttcgacca aatcgccgcc ttcaatttct gcacgcatca ctaataaatc
      301 aaaccctgca cggaatttga cgtgttcaag ggtttgttgc ggtcggttgc cacttcgttt
      361 catcatctga aattgcaatg cccaaatatc tcgaatgact gaagtgtgtc tgcggtgcaa
      421 cgcaatcgcg ttgcaacttt ccgccagcac atcatttgct gccagcatca ttgcatcgta
      481 gctgttgaag ccaccttcgt ttttaagctg ctccattttc tcccggagcg gataccaaag
      541 tagtgcagca aataaaaacg caggatttac cggtaattta tcggcaattc gctcatcagt
      601 tgaatttaac gctttggtaa tcattctttc ggcataggaa tcacctttgg cagtaaaaaa
      661 tgccggcaat accggaaaca agaattgcca tagttcatat tcacgcaaaa gctgataagt
      721 tttcagccct gcaccggttt gtaataattt gagtgattcg tcaaacaaac gggctgccgg
      781 aatgttggtt aacaacaccg ccatatcccg aattggggag gccgtggctt tatccaagaa
      841 catatccaac ttcgccataa aacgaacggc tcgcaacatt ctcaccggat cttcttggta
      901 acgaatagta ggctctccga taagcgaaag tttacctgct cgtaagtctt caataccgtt
      961 gaagaaatca aaaataagat tgcgttttat atcgtaatat aaggcgttta cggtgaaatc
     1021 acggcgctct gcatcttcac ggagcgtgcc gtaaacatta tcacgcagta gcattccttc
     1081 atcgctgact ttagaaattt tgtcattgcg atgagattcg tgtcctgcac ggaaagtcgc
     1141 cacttcaaaa atttcacgcc cgtaaacaat gtgtgccaaa cggaaacgac gtccgattaa
     1201 tcggcattgc ctaccaaaaa tacgctgaat ttcttcaggt ctcgcattag tcgcaatatc
     1261 gaaatcttta ggctttttct ccaagagtag atctcgaata cagccaccca caatataagc
     1321 ttcataaccg gcaatctgca atttatcgac aatcgaaatc gcatttttag gaaaatcctt
     1381 cgggctaatt ctatattggc tggcattcac cgcaaatttc ttgtgtaaca aatgggaggg
     1441 aggattatct tgttgtttct tcccacctct ataagtcttt tggtccgtta agttcggggc
     1501 tgggcgttta gaacgggaag ttttgggttc tctttccgct cttttttcac tctctacctt
     1561 ttcatttttc tgagccgact ttttaggctt ggaaaataat gatttaatat aattaaaaat
     1621 aatacacctc taaatagtaa atgcctctta aaaaagaggc accctgtaaa gttgattata
     1681 agcctagctg cttttctcga atttcagcta aagttttaca gtcaatacat aaatcagctg
     1741 ttgggcgtgc ttctaaacgt ttaatcccaa tttctacgcc acaggtttcg caataaccat
     1801 attcatcttc ttggatttta tacaaggttt gctcaatttt tttcaataat ttgcgttcac
     1861 gatcacggtt acgaagctct aaactgaact cttcttcttg actggctcgg tcgttcggat
     1921 cggcaaagtt agtcatatct tcttgaagct ggcttttggt acgctccgct tcttccataa
     1981 tttgactgtg ccacgctttc agaattttgg tgaaatgctc acgttgagct tcattcatat
     2041 attcttcgtc ttttttaagc tcataaggct caacgcctgc taatgctaat aaaccgagtg
     2101 aggttttagc cgcttgagtc atagatattt actccttgtt ccctgtttat tggaagtttt
     2161 attgttaaaa ttattgcccg aatacagact aacttccttg taaccgcact atacgaaaaa
     2221 atcaacatct tcgggtttta aataatggcg gctataaata acacaaaaac gactcattaa
     2281 tcaaatgaat ttgcaaaaat tttgcattca aattgatttg attgcacaaa aaaatagccg
     2341 aacatccatt cggctatttt tcaaacactt tttaccagct ctttgaccaa tcggctaacc
     2401 atttttggat agtttcagta gtcggtggcg taaattcaat ggttttatag gttggcattg
     2461 ccgcaaaagc cggatcaacg ccgtcggtta aaaccggatt cattacgttg tggtaagaaa
     2521 taatatgttg cgattccggt ttgtgcaaaa attcaagaaa ttgatttgcc aatgcttttt
     2581 gttggctgct tttgacaata gccgcatttt ctacttgcac caaatgccct tcctcaaacg
     2641 gagcagccac atatttatcg gtgttttcgt gccacttatg atataaaggc gatgtagtat
     2701 agcttagtac caaatccgac tcgcctttta agaacgctcc ataagtttca gaccaacctt
     2761 tgcctaccgt taccgtatgt tttgccaatg ttttccaagc agttggagct tcatcgccat
     2821 acacttgatt tacccacatt aagaagcccc gcccaacgct gctggtacgt gggtcttggt
     2881 aaatcacttt taaatcttgg cgttccacca actcttttaa actttttggt ggatttttca
     2941 gcttctcttt atcgtaaata aaagcataag aacccgtgtc ataagggaag aaaattttct
     3001 gttgggtttc taatttggtc tttgcttcaa actcggcaaa caagccactt ttttcggctt
     3061 caggttgcag gaaattatca ataccgacca ccacatcagc tttggttttt gctccctcca
     3121 aacgcaagcg gttgaacatc gttacgctat cgtcaagcgg aacaaatttg acatcacagc
     3181 cacactcttt ctcaaaattc tgctctaatt taggagcagg tccatattcc gaagcaaacg
     3241 agctgtaagt gtaaacggtt aaggtcggtt gattggcata aacacttgca cttaataacg
     3301 caagtgaaga gaggataaat ttcataacaa ttccttctaa attaaatgaa tagaaggatt
     3361 taagaaaggc aagttgctat ctcaaatccc tacgctgata ttaatcagat caggttctat
     3421 gggtatttct cagccacaag cggttaaatt tcggcaaaaa tttgcaaatt tttacgcaaa
     3481 aacaaccgct tgttgcaccc caatgagaac ggcttcattg taaaaagttt gcaggcagat
     3541 ttcaaccaaa gcaaacgttt gctatttgta ttaaaaatcc gtatactacc gccctagtcc
     3601 aaacaaggaa ctagaaatga attgcacaac atcggttaaa tcaaattccc aacagctacg
     3661 cttcaacttt agaggcgtag ctgtttttct attcatttca aaccattcaa aatcgctgtt
     3721 atagcgattt tttttcgcac gcttgcaatt accgcaagcg gttgagccac attaacagag
     3781 agaaaaggaa taaaaatgag ccaattgatt cgtaccctaa aaggacatat tcgtgatgaa
     3841 atcatcaaaa aaggcggttg ggtaaatgca cacgcccacg cagatcgtgc atttacgatg
     3901 acgcctgaaa aaatcaccgt ttaccaaaat gcgaatctac agcaaaaatg ggatttggtc
     3961 gatgaggtaa aacgtaattc aagcgtagaa gattactacc gccgcttttc ccaagcgatt
     4021 gaattgatga tttcacaagg ggtaacggca tttggtactt ttgtggatat cgaccaagta
     4081 tgtgaagaca gagccattat tgccgctcac aaagcccgtg aagtatataa aaacgacatc
     4141 atcttgaaat ttgctaacca aacgttgaaa ggcgtgattg aacccaatgc gaaaaagtgg
     4201 ttcgatatcg gctcggaaat ggtggatatg attggtggct tgccttaccg tgacgaactg
     4261 gattacggca aaggcttgga agcaatggat attttaatgg ataaagccaa atcgctcggc
     4321 aaaatgctcc acgtccacgt tgatcaattt aactcaccta aagaaaaaga gaccgaacaa
     4381 ctgtgtgata aatcgattga gcatggtatg caagggcgag tggttgccat tcacgggatt
     4441 tccatcggct cgcacccgaa agaatatcga aaaatgcttt accaaaaaat gcgtgatagc
     4501 caaatgatgg tcattgcctg tccgatggcg tggattgata gcggacgtaa ggaagattta
     4561 caaccgttcc ataacgcttt aacccctgct gatgaactca ttcctgaagg cattactgtt
     4621 gctatcggca cagataatat ctgtgattat atggtaccgc tttgcgaggg cgatatgtgg
     4681 caagaactca gccttttatc agcaggctgt cgttttacta acctagaaga aatggcaaat
     4741 attgccagta tcaatggtcg aaaagtactg ggtttagtct aactgaataa acgaaaggcg
     4801 aaccctaggt tcgcctttaa taacagcata accgattatt taaacaagtt acccaaaatc
     4861 ccttttgccg cttgagctaa taagtcattt gcatcaaagt tttttaagtc ttcaacatta
     4921 ccattcgggg ttaaagtatc cacgattttt ggcaagaact gggctaataa atcgctcgca
     4981 tcagcagcat caacacccgc ttcttgagcc acattttgaa tgttttcttg cccgaacaca
     5041 ttactgattt gttcgcccga aacaggtgca ttgtcttcat cgctgctgat ccaagtgcgt
     5101 aacacatcat ctaatccgcc ttgttggaat ttggcaatca gcccttcaat cccaccttgt
     5161 gaggcgagta aattttgaat taattgagtt gcagttgatt ggttattgcc actcaatact
     5221 gaagaagcca atgaacctaa aatatttcct aacataattt ctcctatgca agcggtacaa
     5281 ttttgcgaaa attttgcaaa aaaacagcaa acttcgaccg cttgtaatca ctttaacgag
     5341 ctaaaatggc tcgcactctt tccaaatctt ccggcgtatc cacccccact tgcggggctt
     5401 ctttcgccgg ttcgatatga attttttcac cgtgccataa ggctcttagt tgctcaaggg
     5461 actcaagctg ttctaattgt gtcggctgcc acgccacata ttgtttcaca aaaccggctc
     5521 ggtaagcgta aatgccgata tggcgtaaat aaccttgagc ggcaacaaaa ctgtcatcac
     5581 aatccggaaa actgtctctg gcaaacggaa tgctggctcg ggaaaaataa agtgccattc
     5641 cgtttttgtc tgccaccact ttcaccgcat ttgggttaaa tagctcttct cgggtcgcta
     5701 atttcacacc gagtgttgcc atattgactt gataagcatc taaattttga gctacctgag
     5761 taacaattac cggtggaatc agcggttcat caccctgaat attcacaata atttcgctgt
     5821 cggcaatctg cattttttcg atcacttccg ccaaacgctc agtgccggaa ttgtgttttt
     5881 ccgaagtcat acagacttcc gccccaaaag attgagcgag ggcttcaatt tcagggtgat
     5941 cggtggcaat aatcacacgg cttgcaccgg caagctgagc tttttcccaa acgtgttgaa
     6001 tcatcggttt gcctgcaata tccaacaacg gcttacgagg caaacggctg gaagcgtaac
     6061 gagcaggaat aattacagtg aatttcatag gctatcctta tgcttcttga gcatctaaga
     6121 aacgttctgc atccagggct gccatacaac ctgtgccggc agaggtaatc gcttggcgat
     6181 agttatgatc catcacatca ccggcagcaa atacgccttc cacagaagtg gcagtagcat
     6241 tgccttccaa acctgatttc actttaatgt agccgttttc aagttctaat tgaccttcaa
     6301 acattgccgt attcggtgcg tgcccaatcg ccacaaagaa accatcaacc ttaatttctt
     6361 cgctcaattc ttctttggtt gattttaaac gtaagccggt aacgcccata ttatcgccta
     6421 acacctcatc aaccgtacgg tctgtgtgca gaataatttt gccttcttca atacgttttt
     6481 gcagacgacc aagcaagatt ttttcagcac ggaagctatc acggcggtga attaaatgca
     6541 cttcacttgc caaatttgcc aaataaagtg cttcttccac agcagtattc ccaccaccga
     6601 ctaccgcaac cggtttattg cgatagaaga aaccatcaca agttgcacaa gccgacacgc
     6661 ctttgccttt gaaatgctct tcagaaggca aacctaaata tttcgccgac gcaccggtgg
     6721 caataattaa cgcatcacag gtgaaagtgt ataaatctcc gtttagcgtg aatgggcgtt
     6781 tagataaatc cactgaatga atatgatcaa acacgatttc agtttcaaat ttttcagcgt
     6841 gcttgagcat tttgttcatt aactctgtgc cggtaatttg ttcaaattcc cccggccagt
     6901 tctcgatttc atcggttgta gtgagctgtc cgccttgttg cataccggtg actaaaaccg
     6961 ggtttagatt ggcacgggcg gcataaacag cagcggtata accggcaggc cctgagccta
     7021 aaattaaaag tttggtgtgt ttagtggtca taatgaatcc tctgaataaa tattaatgtt
     7081 tattttagcc taaatttcag ttcaactcta ttttcgtttt cgctcaactt ttttcaattt
     7141 tgtgacaaag ttaacagaaa tccgactgtg tttcttgtaa attgtgacag tctaattcat
     7201 ttaaaagggg caaaaagatg aaaaaattaa ttaattcaat agaaaccgtc ttagatgagc
     7261 aactttctgg ttttgtgaaa gcacattctg atttaacact taacacagaa cctgtctata
     7321 tttaccgcac aacgccaagt aatcaaccta aagtagccct tatttccggt ggtgggagtg
     7381 ggcacgagcc aatgcacgcc ggttttatcg gtaaaggaat gttagacgga gcttgccccg
     7441 gagcaatctt tacatctcca acgccggatc aaatgtttga atgcggttta gcggttgatc
     7501 gtggcgaggg tgtattgcta attattaaaa actataccgg tgatgtgctg aattttgaaa
     7561 cagccaccga gctacttgca gatagcggtg taaaagtcgc aaccgtgttg gttgatgatg
     7621 atgtggcagt caaagacagt ttatatactg ccggccgccg cggtgttgca aatacagtat
     7681 taattgaaaa gctgttagga gctgcagcgg ataaaggcta taacttatcc gaattagcca
     7741 aattagggca tcagttaaac aataacgggc attctatcgg aattgcctta tctgcttgta
     7801 ctgtgccggc tgccggtaag ccatcattta ctttagctga aaatgagatg gaatttgggg
     7861 taggtattca cggagaaccg ggcattgaac gtcgcccctt tgaaaatctg gatagtaccg
     7921 tgaaacaaat gtttgagacc ttaattgctc acggcgatta tcaacgtaaa attcgccatt
     7981 ggaatacaca aacccaacaa tgggacgaaa tcgaagaaag caaagtggca ttgaaaaaag
     8041 gtgatcgtgt tattgcttta gttaataact taggagcagt gccactatcc gaactttatg
     8101 ctgtttatga tgcgttagaa aaagagtgcc aaactttcgg cttaagcatt gaacgcagtc
     8161 ttgttggatc atattgcacc tcgctggaca tgcaaggtat ctctatcaca ttactgaaag
     8221 ttgatgagga tattctcaaa ctatgggatg ctccggtaaa tacaccggca atgcgttggg
     8281 gtgaataatt agattgaaag gataagttat gacaatatca aaacaacaga tcttaaaatg
     8341 gcttgaaaat tgccaacagg taatgactga aaaacgggat tatttaaccg aattggatac
     8401 cgttattggc gatggcgatc acggtttaaa tatgcagcgt ggatttagta aagcacttga
     8461 aaaagcccat accgtgcaag ataaagatat cggcaccctg cttaaaaccg ttggaatgac
     8521 attattatcg caagtagggg gagcgagcgg ccctctctat ggttcatttt ttattaaagc
     8581 ggctcaaggt gctacaggca aagagcaact gagctttaac gagttactgg cacttctaca
     8641 gcaaggagca gaagctatcg tacaacgtgg acacgcacaa gttggcgata aaacgatgtg
     8701 tgatttatgg ctgccattac tcgaagactt agctcaagcc gatagcactc aaccaattgc
     8761 tcaaatctta ttacaagcgg tcgaaaaagc agaaaatttt gcaaatcaaa ccgctccatt
     8821 aatggcgaaa aaagggcgtg ccagttactt gggcgaacgc agtatcgggc ataaagatcc
     8881 gggagcaacg tccagctact atatttttaa agccttagct gaagcattac agtaggaggg
     8941 gtaaatggtt aatttagtga ttgtttcaca tagtgctaaa ctggcggaag gcgtggggga
     9001 aattttggct caaatggcac aggggggctg taaaatcgcc cttgccggcg gcattgagga
     9061 tacctcaaat cctatcggca ctgatgcggt aaaaataatg caagcgattg aatcggtatt
     9121 tagcccagac ggtgtgattg tgtttgtcga tttaggcagt gcaattttaa gtacccaaac
     9181 cgccatagat ttactggagc cggaacttgc cgaaaaagtg atcattagct acgctccttt
     9241 agttgaaggt gcttttgctg ctgtagttgc agcctctgcc ggcgatagtt tagaagcggt
     9301 attaaatgaa gcgaacgaag ccgctgctct taagcaggaa caagccaatt agtaaaaatg
     9361 ccgtatcgaa aaatacggca ttttagtttt attagtacaa tagtgaataa agctgtcttc
     9421 taaatttcgg cacaatcggg tctgtaccca gtgcagataa gattgccaga aattggcttt
     9481 ttacctcgcc gttcgctaca tttaaatccg attttaacca atcaaaaagt aacgtgagtg
     9541 cttcttcatt tttatgagct tggtgtagct gatttgccaa tttaatcgca atttcagggg
     9601 ttttgctgtt cgcataatcc gcttgaagct gttgtaattc gggcgattcg ctggcttgtt
     9661 ccaataattc gatttgggct tgcaaaccgt gccaacggct gtctctgtct tgaatcggga
     9721 tcgctttaag gatttcacga gcctcttcca cgcttttcat tgcaatgtaa gtttcggcat
     9781 agagcaaagc gaaatcgctg tttttcttat cggtcatttc ccacgctgat ttcagcagcg
     9841 gcaaggcttc ggtataacgc tgatcttgca ataattccaa ggcttgttga aatttcagct
     9901 cttcctcttt cggtaaaata tttgccaacc gaatccgtaa ttcctgctca cttactgcac
     9961 cttgaattgc atcaatcggc tgaccgccgg caaacaaata gagcgttgga atttgttgaa
    10021 tgcgaaattg agcagccaat gcttgttgct catcacaatt taccgtcgcc aataaaaatt
    10081 ggttagggtt ttcatccgaa atacgacgta gtaaggcatc catttccaat gaagccactt
    10141 cccttggcga aataaaatga aacaccgtcg gcacttgtgc cgctgctgcc catacttcac
    10201 taaaattctg ctcgtttaca tttacaaagt agtccataca aaaccttctt aagctaaaaa
    10261 ttacttttta tattgtagca ttaataagaa gtttcaatag gtagcccgtt tctctcccac
    10321 gcttcaaaac caccttttac actataaaca ttctcaaacc cttgttcgat taaaaactgt
    10381 gctgtacttt ggctactcac tccgtgatag caaatcacaa ccaccggttc gtcataatcg
    10441 acctcgtcta aaaacttacc ataactctgg ttggttaaat gaaacgcatc ctgtgggtgg
    10501 ctgtaaacat aacggcgggc atcacgcaca tcggcaagca ccgcaccttc gttttcgatt
    10561 aattcccaag cctgttgcgg gctaatttct ttaaatgtag tttccataat cactttccct
    10621 caaaaaacgt gcaactatac cataaaattc taaaaagaat ggctattatt ccaagttgaa
    10681 aatttgatgt ttgtcagata taattgcaaa ctattttcaa atgcactgat ttaaaactac
    10741 cacagtttga ggattttatg gagcaattat ttcaattttt agaacatcat actgactttg
    10801 ttatattagg attactaggc ttattaagcg taatctgcct ttggaaagct attgaacgta
    10861 ttatgtttta ttccaaagta gatatttcaa aatacgatac cctcgaagaa ttagacatcg
    10921 atttaaccca caatttaact accatttcga ctatcggctc taatgcccct tatatcggct
    10981 tattaggaac cgttgtcggt attcttttaa ctttctatca tttaggtcaa tcaggtggtg
    11041 atattgatgc ggcctctatt atggtaaacc tttctttagc tctaaaagcc actgctgtcg
    11101 gtatcttagt ggcaattatt gcaatggttt tctacaatgg ttttggtcgt aaagtggaag
    11161 aaaacaaact gaaatggttg gcattacaat caaaaaagcc tcatcaagcg gtgtgattta
    11221 gtccattttt tgcaaattaa taaggcagaa caatgaaaaa attcgatgaa atcaacatca
    11281 ttccatttat tgatattatg ttggtattac tggctatcgt gttagttacc gcctctttca
    11341 tttctcaggg taaaatccaa gtaaatgtac cgaaagcaag tacaacacag ccaatgaaag
    11401 cagacgattt agcgaagctt ttaaccatta ccgaaaataa tgagtttttc tttaatgatc
    11461 aagcaataac taaagagcaa ttaattgctg aagttgccac ttgggataaa agccaaaaag
    11521 tgtcactcaa agttgatggt gcagtcgcct tcgaaaaatt tgtggaatta accgatatcc
    11581 tttctgcaaa tgaaattaaa aatgtagcaa tcattactaa aaaagaaact gctccagcac
    11641 cttcaagcac acctggtagc cctgcacaag tgccagcagt cgcaccttaa atagggtaaa
    11701 caggtatgaa aaaaaaacat tctcgtatcg gcttattggc atctttggct attcacactg
    11761 ccttaattgg tggcactttt gcttggttac ataaccacaa agtggaaaaa attgccgagc
    11821 aaaactctct ttctatggaa atggtggcag cattattaga gcaacctcag gttgccgttg
    11881 ctccggatcc tattaccgaa gaggtcaaag aaatcgaacc ggagaaggca gaagtaccac
    11941 ctgaacctaa gcttactccg gagccaactc cagagccgga ggcaattcca gatcctacgc
    12001 taaatccgaa aaaggaaaaa ccgaaagaag aaaagcgtaa agagcgacac caccataaac
    12061 ataagcatcg tcacaaagag aaaagggaag agctacaaga gcaagaaaaa ccgaaagata
    12121 agcctaaaga gcgcccgaaa catcatcaca agcatattaa agcattagag cgcgggccag
    12181 agataaaaca aggtattgtc gctaaagcga ttccaaatgc ggctgaaggt gaaaaagtgg
    12241 ttgcgggggt tgttggaggg caaaaaaacg gttcaccaaa ttcaacctca actacaggtt
    12301 cagcaaatac tggggctagc agtggtaatg ccagtgaact tgctgcttat aaagtcgcat
    12361 tacagcgagc attacagcgt aaggcgaata actcttaccc tcagcgtgaa aaaatgatgc
    12421 ggaaaaccgg tacagttacg attaaattta ccattacatc gtccggttca attacaaatg
    12481 tatccgtact caattcatcg ggaaatagta atttagataa cgctgcggta cgcagtgcag
    12541 aaggtttgag tatgcctccg ccaccggcag gcttccctcc tacggtaaca gtaccggtaa
    12601 gatttgccct tgaataaaaa cagccctcaa aagcaatgta cttttgaggg ctttttattg
    12661 caaaaaattg actaaaaatg accgcttact gcttgcgaat atcgccattt ctcacattcg
    12721 gactcacttc acccgtacga gtttctgtgc ttaacgaggt agaatttact ttcgtaaaag
    12781 tataatccgc tttgctgaat tcacctgttt gattatttaa gaagaaattg cggtactctt
    12841 ttacccataa tgccgttgcg ccacaaactg ccacaggcat aactactaaa ttcacaaatg
    12901 gaatcatcgt gaataggctc actaaagcac caaaggtaaa atttaaagtg cgagactgcg
    12961 acaacgcatt tcgcatacgc tgaaaactga ttttatggtt atcaaacgga tagtcacaat
    13021 attgaatggc gatcaaccaa gcaccgaaga taaaagtcag cacaggtgct acggtttgcc
    13081 caaccacagg caaaaagcct aatacaaaaa gagcaatcag ccttggaatg ctatacatca
    13141 ttttttgcca ttctcgtttc agcattcgag gcacatcttt taacattgca ccgaatgaca
    13201 tatcgcccaa aggctcgccg gtaagttgca gttcgacttt ttctgctaat aacgcattaa
    13261 agggagcagc aacaaagtta gccaaggtag taaaagcaaa ataaaatccc actaatatca
    13321 tcaaaaaaat cagtggaatc agtacaaagc taagccattc cagccagctt ggcagtaaca
    13381 tatccaacaa gccaccgata ttggcaaaaa atccccaaat cagccctacc atcagcacgg
    13441 tattaataag aattgggaaa atcacaaaag gcaaaaattg acgttgtaac aataaccgcc
    13501 agccatatat aaaataatga aagcctaatc cgacttgatt ttgcggctca gaaaaaactg
    13561 aagacataat gattccttat tttctatcaa aattaattag aaatatggta agctacgcac
    13621 attattttcg caaatatgaa aatacatttt ttattgatta tttgataaag aggacacaat
    13681 ggaactgcat attatttttt ttattcttgc cgggctactg attgctgtac taattggatt
    13741 tagcctttgg tccgctcgtc gtgaaaaatc aaatattttt tcaaatacat tctcgaccag
    13801 acctattgct gtgccaccac agaataataa aatgagcaat agcatcccaa cctcattaca
    13861 gccacaaaat gaggattatc aagcaatgtc tgtgccggaa gaaaatccgc aacagataca
    13921 acaggaagtt gaaagctcgc tgcaaaatat caaaatcagt ttgccgggcg gaaatcctca
    13981 gcaacagcct gagcaaattc agccgataca atcggtagca gaaccaacgg ttagcactta
    14041 cggtcagaaa atttctaatc agcaacatat ccaatacaat acgccggatt ttgcaaaccc
    14101 aatggtgaac acagtggtgg aagaaacacc tcaagcagta gctatcgaac ctcaagaaac
    14161 ccaatcggca gaggaagaaa atccatttgt aaccctctat ctttacgcac cacaaaatga
    14221 agctttccgc ggtgattttg tagtacatta tttagaagaa gttggcttac gttttggcga
    14281 acaccaaatt ttccaccgcc atcaacatat tgatgattca ttaagtccga ttattttcag
    14341 tgcagcaaat ttaatgaacc caggaacctt tgatttaaat aatatctcaa actttgcgac
    14401 agctggttta gtgctattta tgcaacttcc aaccgaaggc aatgatgtgg ctaatatgaa
    14461 gttgttaatc agtaacgctg aacacttagc agcatcgtta gggggattct tatatgacga
    14521 taatcaacag ccttttggcg aagaatcccg ccaacgttat atgcaacgtg tgatgcacta
    14581 attgcgaaaa aattaataaa tccgaccgct tgtacaagcg gtcttttttg tagagaattt
    14641 taccaatgtc tttatttaac caaacctctt tatttgaaga gaatgccacg cccgagttag
    14701 tcgagaaaat cgaaactatt cgccaaacat tgcgtcaata cgagtatgaa tatcacgttt
    14761 tagataaccc aagcgtaccc gatgccgaat acgaccgttt aatgaacgag ctgaaaaatc
    14821 tggaatggca gcacccggaa ctgattagtg ccgactcgcc gactcaacgg gttggtgcca
    14881 aaccgctggc aggctttgct caagtcaccc acgaaatccc tatgctttcg ttggataatg
    14941 ccttctccga tgaagactta gatggctttt tacgccgtat ggaaagctat atcaccgcct
    15001 ctccttcgca actggaattt tgttgcgaac ctaaattaga cggcttggca gtgagtattt
    15061 tatacgaaaa cggtatactt acccgagctg caacccgtgg cgatggcaca accggcgaag
    15121 aaattaccgc taatatccgc accattcgca atattccgtt aaagctcaac acgactaatc
    15181 cacctgctcg attagaggtg cgaggcgaag tctttatgcc acaatcaggc tttaatgagc
    15241 tgaatgagaa agccttagct aaaggcgaaa aaacctttgc caaccctcgc aatgccgcag
    15301 caggttcgct tcgccagtta gatccgaaaa tcacccgcca gcgtccgtta gtgctgaatg
    15361 cctacggaat tggggtgtat gaaagcgatg acgaactgcc agccacccat ttcgaacgtt
    15421 tgcaatggct gaaatcgctt ggtattcctg taaataatga aatccgcttg gttcaaggca
    15481 aagctgctct cttggcttat tatgcggaga ttcaggctaa acgcccaaca ttaggctatg
    15541 atattgatgg cacagtattg aaagtaaatg acattgccct acaagagcaa ctcgggttta
    15601 tctctcgctc ctctcgttgg gcgattgcgt ataaatttcc ggctcaagaa gaaatgacaa
    15661 tcctcaaaga agtggacttc caagtgggaa gaacaggggc aatcacccct gtggcgaaat
    15721 tagagcctgt ttttgtcgct ggcgtaacgg taagtaacgc aacgctacac aacggtgatg
    15781 aaatcgagcg acttggtatt gtgattggcg atacggtaat tattcgccga gcgggcgatg
    15841 taattccgca aattacaggc gtgatgttag aacgccgccc ggaaaatgca aaaaaaattg
    15901 aatttccgac cgcttgtcct atctgtcact ccgctgttac tcgggtagaa ggcgaggcag
    15961 tagcacgctg taccggtggt ttattctgcg aagctcaacg taaagaagcc ttgaaacatt
    16021 tcgtttcacg caaggcaatg gatattgacg gcgtaggcga aaaactaatc gagcagttaa
    16081 tggctcgaga gctgattgaa acgcctgccg atctgtttaa gctcgatcac actaccctaa
    16141 tgcgattaga gcgaatgggg gaaaaatcgg ctcaaaatgc tctcaacagc atcgaaaaag
    16201 caaaaaacac tactcttcct cgctttttat tctcgttagg tattcgtgaa gtgggcgagg
    16261 caaccgctct caacttagcc aaccattttg gtagcttaga ggcgatccgc caagccactt
    16321 ttgagcaatt aaaagaagtg caagatgtgg gcgaggttgt tgccaaccgt attgtcagct
    16381 tctggcaaga accgcataat gtggaagtgg tggaagattt gatcgctcaa ggtgtacgtt
    16441 gggaaaacgt tgtgaaagcc gaaattgtag ataacccgtt aaaagataaa aacgtggtct
    16501 taaccggcac gctaacccag ctcacccgtg atgaagccaa agccctatta caaagtttgg
    16561 gctgtaaagt ctccggctcg gtttccagca aaaccgatta cttaatcgcc ggcgaaaaag
    16621 ccggttccaa gctggcaaaa gctcaagagt tgggaattca gattttaagc gagcaacaat
    16681 ttattgagct tactcaacat taacaaacaa aggggcagat gattgaaatc tgccccttat
    16741 tctatctatt gaatgcttaa aagcattaac caatcaattt aaactgctga gctaaaaaca
    16801 gtaacgtaaa gctagttaag taaattaagc cgataactgc caaccaaaat aaccagcctt
    16861 ttgcacgatg ctcacctttt agcactaaag agaaatttaa ccacgcaaaa acaggggtag
    16921 aaacaaatga tgaaatcatt gcaaattgta acattttcgc cacatcactc ataaattggc
    16981 taataattaa aatgccgatg agtgcagtta ccgtcatcca aatacttaat tctgtttgtg
    17041 aatgttgctt acgcccgcta attaaacgca acgcttctgc attcgctcgt gaatatccat
    17101 caataacagt gatcgttgtg ccgaacatac acataaatgc aataaatgcg attaaaagct
    17161 tagaccagtc accaatcgca aaagcataca ttttgattaa ttgagcaata taggcaacac
    17221 cggcttgttc tactttctca cctgaaccgt actgaactaa cgcacctaat gctaaaaaaa
    17281 caatcgccaa aattgccgtg ccgatataac ccacattaaa atcaaaaata ccgtcctcat
    17341 aattcacttt acttaaacgt tttttcgcaa taacccacat tgagttaatg gcagaaattt
    17401 caattggtgc aggcatccag cccattaatg caacgatgaa cacaagtgct gctaaattcc
    17461 acggcgaagg ctctacgaaa tctggagcaa tttcaacgcc accttttaaa gcagcaatga
    17521 tcacggcaga taccgtagag atggttaaag caatcataat ccatttagat aatgtatcta
    17581 agaatttata ttgccctaga agtaaaatac cccaagtgac cacaatcaca atagttcctg
    17641 ccattggaat gcttaaatct aacggaatac ctaaagcaga aaaaataaat tttagaatcg
    17701 ctgctgaaac aatcgccacc gcagctgtgt taatcattgc agcaaaaacg ttaaggataa
    17761 agaaaataat aagataaaac tttccttttt ctttgtagcc ttccaataaa gttttaccgc
    17821 tagctaaggt atattgcaca ccaaagcgga aaaacggata tttaaataaa ttggctaaca
    17881 gaataataat cgccagctgc cagccataaa tcgcaccaga ttgtgtagag gcaataatat
    17941 gcgaaccacc cactgccgca gatgccatca aaatccccgg ccccattgca gcaaatttac
    18001 tttgccaagt tgaatgttct atttgttgtg tcatatagct ttccttatca cattactatc
    18061 aaaacaagat ggtttcattc tacttataaa aaccaaataa aaacactcct ctagaaaaga
    18121 tgttca
//
LOCUS       DANLUA010000027        18124 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLUA010000027 DANLUA010000000
VERSION     DANLUA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18124
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(76..4062)
                     /gene="hrpA"
                     /locus_tag="PXB44_000715"
     CDS             complement(76..4062)
                     /gene="hrpA"
                     /locus_tag="PXB44_000715"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL5281225.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4166..4618)
                     /locus_tag="PXB44_000716"
     CDS             complement(4166..4618)
                     /locus_tag="PXB44_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL5281226.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4681..4950)
                     /locus_tag="PXB44_000717"
     CDS             complement(4681..4950)
                     /locus_tag="PXB44_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281227.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5048..5644)
                     /gene="grpE"
                     /locus_tag="PXB44_000718"
     CDS             complement(5048..5644)
                     /gene="grpE"
                     /locus_tag="PXB44_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL5281228.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5866..6960
                     /locus_tag="PXB44_000719"
     CDS             5866..6960
                     /locus_tag="PXB44_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL5281229.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7096..8487
                     /locus_tag="PXB44_000720"
     CDS             7096..8487
                     /locus_tag="PXB44_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL5281230.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8546..12070
                     /gene="recB"
                     /locus_tag="PXB44_000721"
     CDS             8546..12070
                     /gene="recB"
                     /locus_tag="PXB44_000721"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL5281231.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12112..12594)
                     /gene="greB"
                     /locus_tag="PXB44_000722"
     CDS             complement(12112..12594)
                     /gene="greB"
                     /locus_tag="PXB44_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL5281232.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12645..13925)
                     /gene="pepB"
                     /locus_tag="PXB44_000723"
     CDS             complement(12645..13925)
                     /gene="pepB"
                     /locus_tag="PXB44_000723"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL5281233.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14064..14885)
                     /locus_tag="PXB44_000724"
     CDS             complement(14064..14885)
                     /locus_tag="PXB44_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL5281234.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15033..15976
                     /locus_tag="PXB44_000725"
                     /pseudo
     CDS             15033..15976
                     /locus_tag="PXB44_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16089..17252)
                     /locus_tag="PXB44_000726"
     CDS             complement(16089..17252)
                     /locus_tag="PXB44_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5281235.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17318..>18124)
                     /locus_tag="PXB44_000727"
     CDS             complement(17318..>18124)
                     /locus_tag="PXB44_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5281236.1"
                     /translation="PFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFATGR
                     NHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDDQNV
                     CLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEALVP
                     IEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGDGMN
                     DVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4898 a         3796 c         3939 g         5491 t
ORIGIN      
        1 ataggtattc gagaaaacaa gcggtcagag ttgcaaaatt ttttacttat ctgaccgctt
       61 gttgtatagg gaatattact ttatttcatt gatcacattc aaaatccgct tatccgaaat
      121 cgggtatttt gtgccgagct gttgagcgaa cagactgacc cgcagttctt cgatcatata
      181 tcgaatttcc agtacctcgt ccggcaccgc tttggatttt ggcagtttgg caaggagctg
      241 tttatatgcc tcttgcactg cttccacccg tagcattttg gctcggtcgg tgttgctgtc
      301 caccaacagt ttatccaaac ggcgttcgat agcggtcaaa taacggtata aatctgcaag
      361 gcgggcgtag ccggttttgg tgacaaagtc ggggtaaatc agcccttcga tttgggtttt
      421 gatgtcgctt aacgcaaacg ccatcgtaaa atcgagcttg ccttttaacc gcttgtttag
      481 ctcgtaggcc agtgtgaggc acttttcaac ctgtttggca atttctacgg ttttatcgtt
      541 taaatttgcc cgcacaaatt cgtgcagttg ggtgaatttt tcctccgtcc agacaaaccc
      601 gccgaactct tctaccagct gatctaccgc acaggcaatg cagtcgtcaa tcagctccaa
      661 tactttgccg aacggggcaa aatataagcc cagcttggct ttgttcggca gtttttcgtg
      721 tagatattta atcggcgatg gcacatttag caacagcaga cggcgaagtc ctgcctgcat
      781 tgcacgagcc tgctcaaatt ccgtttcaaa cagtttcacc cccacagccg tttgttcatc
      841 gacaatcgca gggtaggctt tcacgctgaa atgggctttt ttctgctcat agaactgtgg
      901 caattcggca aaattccaaa tatgcacacc actttgctca atgccgtcat cggcaatgtt
      961 ggaaagggtg ttctgcacct catctttcaa ggcaaatttc agctcatcaa ggttttcgct
     1021 ctcttgcagt ttcttgcctt tatcatcaat cacacggaaa gtcatccgca aatgtggcgg
     1081 cagttggctt aaatcccact gttcaggctc gaccaataca cccgtcattc ggcgtagttc
     1141 gtggcttaga ttgtccagca acggtttttt aaacggctcg gctcgcccta aaaaggcgtc
     1201 cgcataattc ggcgccggca caaaatttcg ccgtagcgat ttcggtaaag atttaatcag
     1261 cgagacgatc agctcgtggc gtagccccgg aatttgccaa tcgaaacctt cgggttccac
     1321 ttggttaagc agcggtagcg gaatatgcac cgtcacgcca tcgtgatctt ttccgacctc
     1381 aaactgatag ctcagtttga gttttaaatc gccttgatgc cagaaattcg ggaaatccag
     1441 ctcgctggca aggttggcgt tgtcgttgat caggaacgat ttttcaaagt tcagcagttc
     1501 aggatctttt ttgcttgcct gtttccacca agtatcaaaa tgcttgctcg acacaacttc
     1561 ggtgccaatc cgctgatcgt aaaactcaaa cagcacctgc tcgtccacca aaatatcacg
     1621 gcggcgggat ttgtgttcca atgcctcaac ttcacggatc aagcggtgat tttctttgaa
     1681 aaatttgtaa ttgttgtgac attcgccttc caccaacgcc gagcggataa aaatctcacg
     1741 gctgacggtt gggtctatcg agccataatt caccgagcgt tgtgccacaa tcggaatgcc
     1801 gtagagcgac actttttccg atgccattac cgctcctttt gacttcgccc agtgtggttc
     1861 gctgtaactg gatttagtga ggtgtactgc caacggttcc acccattccg gctcaatgcg
     1921 agcgaccatt cgtccccaaa gtttggtagt ttccaccagc tctgcggcca ttacccattt
     1981 cggctgtttt ttgaaaagtg ccgagttcgg gaaaatgaaa aagtgggcat ttcttgctcc
     2041 taaatagtgc attttttcat tatctttcaa gccgatatgc gacaacaagc cggcgagcag
     2101 ggcggtgtga atcggctgat aacttgccgg ctcactgtta atttttaagc ccatttctct
     2161 aactgcaagg cggagttggt ggtagatatc ctgccactcc cgcacacggt tgtagttcaa
     2221 ataatcctta cggcagagat tacggaactg gtttttcgac agttcttttt gttgggcttg
     2281 gataaagtgc cacaaattca caaaggctaa gaaatcgctc tctttatcgg caaaacggcg
     2341 gtgtttatcg tctgccgact gctgtttttc ctgcggacgc tcacgagggt cttggattga
     2401 gagagctgac acaatcatca tcacttcgtg caacgaaccg tttttcgccg cctcgatcac
     2461 cattctgcct aagcgaggat cgactggcag ttgggcgagt tggcgaccga ttggggttaa
     2521 tctaaaccca tccccccgtt tacgggggga ggttgggagg gggggagtgc catcattaga
     2581 acggatttcc cccctcccta gccctccccc gcaagcggtg gaggggattt tttcatttgg
     2641 aataatcgcc tgtaattctt ccaacaaccg aatcccgtcc tgaatttgtc ggctgtcagg
     2701 ggcatcaaca aacgggaaat tggcaatatc actcaatcca agcgaagtca tttgtaaaat
     2761 aaccgctgca aggttagttc gtagaatttc aggatcggta aattgtggac ggctgttgaa
     2821 atcctcttct gaatagaggc ggatacagat cccttccgag gttcgcccac aacgcccttt
     2881 ccgctggttg gcggaggctt gcgaaatcgg ctcaatcggc aagcgttgca ctttggtgcg
     2941 gtagctataa cgagaaatac gagccgtgcc ggtgtcgatc acatatttaa tattcggaat
     3001 agtgagggag gtttctgcca cgttggtcgc cagaataatt cggtttagcc caccgcttgg
     3061 ctggaaaatc cgttgttgtt cggcagccga taagcgggcg taaaggggta aaatttcgct
     3121 atgtcgcagt tcctgctttt gcaaggcttc ggcagtgtca cgaatttccc gttcaccgtt
     3181 cataaaaatc agaatatcgc cacgcccttc ggcttgcagt tcgtccaccg catttagaat
     3241 accttgcagt tgatcttgat cttccactat cgggcgataa cgcacttcca ccgggaatgt
     3301 ccgaccagag acttcaataa tcggggcgtt gttgaaatgt ttggaaaaac gttctacatc
     3361 aatagttgcc gaggtgatga tcactttcag atcggggcgt ttgtgcagaa tttgtttcag
     3421 ataacccaaa ataaaatcgt tgttcagcga acgctcgtga gcttcgtcaa taatcagcgt
     3481 atcgtactga tttaaatagc tgtcgtgctg gatttccgcc agcagaatac cgtccgtcat
     3541 tagcttgatt aagctgtttt cactgacttg atcgttaaag cggactttgt aaccgaccgt
     3601 ctcgcccagt tccgatttca gctcttcggc aatgcgagtc gccaccgaac gtgcggcaat
     3661 tcggcggggc tgggtatgcc caatcaagcc cttcacccca cgcccgagtt ctaagcacat
     3721 tttcggtaac tgggtggttt tgcccgaacc ggtttcccct gcgatcacca ccacctgatt
     3781 ttcgctaatc agttttaaaa tttcctcacg gcgagcggaa acaggcagat cagggtagtc
     3841 aattttcagg ttttgggcgt gctgagttcg tgcgaaaaaa cgtgcctgac aagcggtgag
     3901 atcttgcgaa atttctgcca aaactgaccg cttgtgatcg atgtttttaa tattttccgc
     3961 cccttttaag cgaccgagca ggcggcggaa atcggcggtg gtgatatttt ccagcttcac
     4021 aagcggtggt ttcttatgat ttttttgcaa aggtgtttcc atttaatttc tcttttgcat
     4081 taagaccttg tgatttattg tagggtgggc gtaagcccac gtaacagaat agggatttta
     4141 tgcgtggact tacgtccacc ctacattagg ttttaccaac aatcagtgac aaaatccctg
     4201 ccgcaatcaa cggcccgacc ggtacgcctt ttaagaacgc cacgccgatc accgtgccga
     4261 ttaaaagccc agttaccaac accggctggc tgcccattaa attcacgccc ctgccaccta
     4321 gccacgctac cagcacgcca gcaattattg agagtgccat tttccagttc aatagttggg
     4381 caagttcggg aagcgtaatt cgccctgaaa ccaaggggct aagtacgccg atggttaaaa
     4441 tgataatccc gatttttaag ccgtattgat cgacaaaggg gataaattta gaaagtaagg
     4501 tttgttgcat aatcagcaat acggcggctg aaatagtaac cgcactgttt tggctgatca
     4561 gccctaaaat aatcagcacc acaagtagaa gtgccactgc gttaaattgt aaagtcatag
     4621 gattaaaacg taaaaaccct tacttcatag aagtaaggga gtggttaatt aaacgttgag
     4681 ctagttaata aatttcatta ttgccattaa aaagccaaga attgtgatat ttaaggtaat
     4741 tgctttaaat atcgtggtat tgatttcatt ttttagcttt tcaagttcaa gttgcaaatc
     4801 agcttttgtt gctagtgggc tttgactttg ctctaaggct tcatcgagag cattagcaaa
     4861 tgcctcagcc atttcaggtg tttgattggc ttcttgcaag cgttttacaa aacgtaattt
     4921 gtcaaatcgt aggctcatag ctgcatccat ttcaattatc ctcttgtggt taagttataa
     4981 actaatttag cataatcaca agcaggaatt ttaggagatt ttgtgatctt cgtcgaaaat
     5041 cgacaattta gtttgccacc atcaccattg ccgggcgaat cactcgtccg tgaagcgtgt
     5101 agcctttttg tagcactgtg ctgacatggt tggcttcaat tccttcggca ggttgcattg
     5161 aaatagcttc gtgcaattca gggttaaatg cctcgcctac ttcgccaatt gccaccacac
     5221 caaatttagc cagtgtattg atgaattctt tatgagtcat ttcaacgccg tcaactaacg
     5281 cttgagtggt ttcatcggtc acggctttat caagggcatc taatccacgt tcaaggttat
     5341 caaccactgt taacaattct ttggagaatt tttctaaagc gaatttgtga gctttttcaa
     5401 catcttgctc tgcacggcgg cggatatttt gaatttctgc ttgagcccta agctgaatat
     5461 cttgctcacg tttgtcggct tctgcaatat aggcttctaa ctcggcaatt cgagcttgag
     5521 cgagagctaa cggttcttct tgaggtgtat tttgttcttc cgcttgagtt tcttcagtta
     5581 caacttcagg ttgaatttct tcttgtttta cttcttcttg taagtgttca ttttgagtgg
     5641 tcatttttta tcctttaaaa gactaatctg gaaatgtaaa tcttccttaa tatagcgata
     5701 aattaaggga tttcaagcat atctctttct ttttgcgaaa ataattcact ttttaatcag
     5761 aaaaggcttg caaaatacgg gggggggctt aaaatgccct agtcttttcc gcccttaggc
     5821 aaggctatat tttttaatca cttttcttta acggagaaaa cttaaatgaa aaaattagcg
     5881 ggtttatttg cagcaggttt agcaacagtt gcattaacag cgtgtaatga agaaaaagcg
     5941 gcagagccaa aagcaactgc ggctgaagca aaacctcaag caacaaatca aacagttcac
     6001 ctttatactt ggactgaata tgtgcctgaa ggtttgttag atgaatttac gaaagaaacc
     6061 ggcattaaag ttgaagtttc aagtcttgaa tctaacgaaa caatgtacgc taaattaaaa
     6121 ttacaaggca aagatggcgg ttatgatgta attgctccat caaactactt tgtatctaaa
     6181 atggcgaaag aaggaatgtt agcggaatta gatcacgcac aacttccggt tattaaagaa
     6241 ttaaaccaag attggttaaa taaaccttac gatcaaggaa acaaatattc attacctcaa
     6301 ttattaggtg cgccgggtat tgcatttaat acggcagatt acaaaggtga aacattcact
     6361 tcttggggtg atttgtggaa acctgaattt gcaggtaaag tacaattatt agatgatgct
     6421 cgtgaagtat tcaacattgc gttattaaaa ttaggtaaaa acccaaatac aactaatccg
     6481 gatgaaatca aagaagcctt tgaagagttg aaaaaattac gtccaaatgt gctttcattt
     6541 acctctgata acccggcaaa ctcatttatt gctggtgagg tttctgtagg tcagttatgg
     6601 aacggttctg tacgtattgc gaagaaagaa aacgcaccgg ttgatatggt attcccgaaa
     6661 gaaggtcctg tgctttgggt cgatacttta gccattccgg caaatgcgaa aaacaaagaa
     6721 aatgcacata aattaatcaa ctacttatta agtgcaaaag tagctgaaaa attaacgtta
     6781 gcgattggtt acccaacttc aaacgtagaa gcattaaaag tattaccgaa agaaattact
     6841 gaagatacag caatttaccc atctgcagag gttttacaaa aatctcaatg gcaagatgat
     6901 gttggtgatg caattgagct ttacgaaaaa tactaccaag agttaaaagc agcaaaataa
     6961 tagctgatta attctgaagc ctctagggag caatctttag aggctttctc ttttgagttg
     7021 caaaaaattt ggcaaaaacg accgctttct aaggtacaat cataaaaaca tacttagatt
     7081 ttgagagatt aaaccatgaa cttaagatgg caagaatatc ctgaaaatgc ccgcaaatta
     7141 tttaaactat ccctacccat ttttatttct cagctttcag cttccggaat ggggctggcg
     7201 gatattgtaa tggctggctt ggtgagtgat gatgatgtct cggcgattgc ggtaagtaat
     7261 tcgatttact ttccgctctt tctgtttgtg tcagggttgc tgaatgcaat taccccaacc
     7321 gtttcttact taaacggctc gagcaatcgc catttaattg ctaaccaagt aaggcagggc
     7381 tattggattg tgtttgcaat gtctattcca ttaatggcaa ttttcctaaa cagccatctg
     7441 attttagact taatgcaaac accggctgag ttttcaatca aatcgcaaca atatttagca
     7501 gtaatggcag ttgggcttat tcctgctttg ctggcggtga gtttacgttg tatgaacgat
     7561 ggattatcta accctaaacc tgcgatgcgg attacctttt tcggtttatt gctgaacatt
     7621 ccgctaaact atattttcat tttcggcaaa tttggcttgc ctgaaatggg agcggtaggc
     7681 tgtggtgtag caaccgcaat tgtgaacttg attatgtttt ttatgttgtt gcattattgc
     7741 tacaccaatt ctgcccaaaa agatattcgt ttgtttgaga aatggattga tgctccaagc
     7801 ggtcaaattt tgctaaaact ttgcaaatta gggctgccga ttgcctttgc gatttttacc
     7861 gaagtgatgc tgttttcgac ctcatcgctg tttctgtcac cacttggttc acaggtggtg
     7921 gctagccacc aagctgcatt gcaaaccagc tcgctatttt ttatgattcc gatgtcgttt
     7981 ggcattgcaa ccaccattat gatcggacaa acacttgggc agaaaaatgt ggagggggcg
     8041 aaaatcttaa gttaccacgc actgattacc gcaaccgctt tagctattat tgccgccatc
     8101 attattattt tgttgaaaaa ttttatccct tttgcgttta ccaacaaccc gatttcgatt
     8161 gcgattaccg ctcatttatt gatttttgcg gcggtttatc aaataccgga tgccattcaa
     8221 gcggtagcaa acggcattct gcgtggttat aaacataccc aaccgatttt atacgttact
     8281 ctgttttgtt attggggggt gagtatgccg ctgggctaca ttttggcaag aaccgattgg
     8341 attgtacaac caatggctgc tcaaggtttt tggttagtct tttgtatcgg cttaagtttg
     8401 gctggtggct tgttattatg gcaaatgcga aaaatacaag cggtgccaaa agcacaatta
     8461 attgcaaaat tagaacggat taaataacag attctctgct atggaacata gcagtgcttt
     8521 ttacttttag aagataactt tgctgatgaa aaacctaaat cctatccaac tacctttgaa
     8581 taaatccgtt ttaatcgaag cgtcggcagg tacagggaaa acctttacca ttgccaacct
     8641 gtatttgcgt ttattgctcg gcattggttg caacccgtta acggtggaac aaattttggt
     8701 ggtgacattt accaaagcag caaccgaaga gttgaaaaac cgtatccgta aaaatattca
     8761 gcaatgtgcc gactttttaa aagatcaagc tgatggtttg gaagtggaaa gtacaaaatc
     8821 ctatcgaaac aaccttgatt ttttggcaca gatttatccg ctcattccga atattcacga
     8881 agcattgtta cgtttaagta ttgccgaacg ggaaattgat accgccagcg tgtttaccat
     8941 tcacggcttt tgtcaaaaaa tgctggttca gtttgcgttt gagtcgggcg tgcggtttga
     9001 cttggattta cagccaaacc aaagtgatct acttaaaaaa ttaagtgaag aagtctggcg
     9061 agagcaattc tatccgcaag atttagccat cacttatgcg gttgccgaac aacttggtac
     9121 gccggaatat gccctcaatg cggtaaggcg ttatttatct actgaattac ctgaaccaaa
     9181 tgcctcttta aatcaagata ttgccgagca tttggctcaa tatcagcagt ttattaatga
     9241 ggtgaaatcc tattggaaaa cgcattcaga tgaactggtt gaaactatcc gccacgattt
     9301 aaagcataaa attacgctgc acggaggttg gtatcgagcg accacttttg aagccggtgt
     9361 ggaagtgatg aaccagtggg ctaattcgca acataaccat ttcccggcag ggctttattt
     9421 attcactaca gagaaaattg cggctggcac gaaaaagagc tgtgagccga tagattcccc
     9481 tcatcttgca aaaaatcagg cttttttgac cgcttacaag gaacgttttg aaaacagctt
     9541 gtcagcaatg ttgcagtatc aatttttaat tgctctcaaa gcaaaattgg cagagtacaa
     9601 acaatcgcac ccacaacgca gttttgatga tttactcaca atgctaaacc aagcgttgaa
     9661 aaatctacac acgggcgaag ccttgtctca gaaaattcgg gcgatgtatc cttttgcgat
     9721 gatcgatgag ttccaagata ccgatttaac ccaatatgaa attttcagcc aaatttttat
     9781 ggcagagaat gcagagaacg gctttattat gattggcgac ccgaaacagt cgatctataa
     9841 attccgtggg gcggatattt tcacgtattt gcgagcagca aagcaggctg atgaacaatt
     9901 cacattgggc aaaaactggc gttcgttgcc tgaagtggtg caagcggtca ataatttgtt
     9961 ttcttttgca aatgataact ctccgtttct ttatgaggat attggctttc acccagtgga
    10021 ggctgatagc caaaaaggtc agttagttag tgagcaaggg cattttgtag gctacatcac
    10081 ggaaaacagt aaattagatg atttggctga aatgtgtgct tatcaaattc atcaacagct
    10141 tgaggcgatg gctcaaggca aattcggagt tgaaaaaaac ggcgaatttc aatcctttgc
    10201 tgcaaaagat attgcgattt tagtccgaaa aggttcacaa gctaagcgaa tcaaaaaggc
    10261 gttggcaaaa cgtaatatca aatccgtcta tttagctgaa tcagcttctg tatttggttc
    10321 ggaaattgca acagaattgt gttggttatt gcaagccgca ctgaatccgt atcattacaa
    10381 ggcattatta tcggcgttag gctcaacctt gtggggctta acggcggcag agatccaccg
    10441 ctataaggaa aatgagcaac tttgggatct gaaagtcaat cagtttgtgg aatacagcaa
    10501 catttggaaa gagcagggcg ttctgccgat gttgcataaa atctacttgg agcagggcat
    10561 tatcgaacgt ttgaaaggtt tggaaaatgc ggatcgcttg attacggatt tattgcattt
    10621 aaccgaaatt ttgcaggaaa cctcgcaagc gttagagtct gaagctgaac tgttgcggtg
    10681 gtatgaaaag caagttaatt cgcccgattt taatgacgag caacaacttc ggttggaaag
    10741 tgaagaggag ctaatcaaaa ttgtgacgat tcacggctcg aaaggcttgc aatatccaat
    10801 tgtttggtta ccttttgttt cacaaaaaag tgagggagac acgcagacag cattaagttt
    10861 atatcgcaat tctcaaggtg cgctggattg gtgtttaggc tcagaagagg caagtttttc
    10921 gctacagtat gaggcttatg cggaagattt acgtttgctg tatgtggcat taacccgcgc
    10981 ggaatctcag cttaacttag tgttgccgaa agagatttcc ggctggtctc cactccatta
    11041 cttgttagca gacggggaaa tacctacagg aaaagtgcct gtttcagatt attttagcca
    11101 aaaacggatt gctgctaatt tggttgaagt agccggggaa ttacctttct cactgtggga
    11161 aggagaacaa tcttcagacg attaccaagc aaaaaccttt agtggcaata tccgtgtggt
    11221 tggtcaaatt accagcttta cttcacttta tgcccaacac gagcgtttta gtgaagggaa
    11281 atcagaaagg gtaatggatt tagcccaaga tcgagatgta catatagaaa ccattgaggc
    11341 aggttattca agtgaaactg ccgctttctc gccattccaa ttcccacacg gtattaaagt
    11401 gggcacggtg ttacatcact tttttgagca ttgccaattt aacgaacaaa ttgatagaga
    11461 ggctgttgct aaagtatgcg agcaattagg gctaagcgag gaatggattg aaccaacagc
    11521 attatggttt gagcgtattc taactacccc tttggcagag gcaaatttct gcttaaaagc
    11581 gattgatgaa accaaacgat taaacgagtg gcaattctat ttacgcttaa aaaatgataa
    11641 agccttacat caattaaatg cgttattaaa gcaacatagc cctttagcaa aaactttacc
    11701 tgaattgcaa ttaccgcaat tagaaggttt tgtgcgaggc tttgtagatt gtatcgtgca
    11761 agtggaggag aaattctact taattgatta caaatcgaat ttcttaggct atctgccaca
    11821 aaattatgcg aaagaacaca ttcaacggga aatgggcaga caacgctatg atttgcaata
    11881 tctgctttac actctcgcct tacaccgtta tctaactgcc cgtttaggtg aaaaatacga
    11941 ttatgaacga gacttcggcg gagtggctta cttgttctta agggcaatga acggcgaaaa
    12001 tgataacggc gtttattttg acaagccgac taagcagttg attgaagaaa tggatcggtt
    12061 gtttgggtaa atatgtgtag gggcgaaaat ttttcgcccc tttatgggta attaactcgc
    12121 ttgctcatac caaatcttat tgatataaag caacacttta ccaagcggag tttctaccct
    12181 aatttcatca tcaacctgtt tgccgattaa ggctcgggcg acaggggagt caatcgaaat
    12241 ccagtttttt gctgggtcga actcatcgca accaacaata cggtactgtt ttgcctcgcc
    12301 ctcttcattt tctaattcca cccaagcgcc gaaaaagact ttgccttctt gtttagggtg
    12361 ataatcaaca atttgtagca cctctagccg cttagataaa aaacgcacac ggcggtcgat
    12421 ttctctcaaa cggcgtttgc cgtaaatata ttcggcattt tcgcttcgat cacccaatgc
    12481 ggcagcatca gacaccgctt gagtcacttt cgggcgttct tcccgccata gaaattttaa
    12541 ttcctgatct aaggcttgcc agcctgaacg ggtaatgtaa tttgatttag ccatagttat
    12601 ttaattgttt atggagcaaa tagtgatttg cccctacgaa gtttctattt tgaaagcaat
    12661 aaattcgcta ctgttcttat tcccagtccg gttgctcctg ccgcccataa atcgctcgcc
    12721 gttttgcgga atgtggcaga ggcgtcaata tgtagccagt tttgctggta gttttctaca
    12781 aagtaagata aaaatgcggt ggcggtactt gccccagccc ccaccggcac agagccgata
    12841 ttggcgatgt ctgcaaagga agaagcaatt tgcccacggt gaaattcttc aaacggtaaa
    12901 cgccagaacg gttcattttc cgttttggca gacttaaata gtgcttcggt taggttgtcg
    12961 tccattgaaa gcaccgagtg gtagtcattg ccgactgcga cttttgctgc tcctgtgagg
    13021 gttgcagcat caataataaa ttgtggattt tgcttgcacg catcaattaa accatcggca
    13081 agcactaaac gcccttcagc atcggtgttt aaaacttcaa cagttacgcc attgcggtaa
    13141 gtgataatat cgcctaattt gaacgcattg tggctgacca tattttccgc acagcagagg
    13201 tagagtttca ctcgtttatt caagcctcga gcaatggcaa aagcaagtga gccggttaaa
    13261 agtgccgctc cgcccatatc ggttcgcatt gtgctcatac tatcgcttgg ttttagacta
    13321 taaccgccgg tgtcaaaggt aatgcctttg ccgaccaagc aagccagtac ctgtgcgttt
    13381 tcatcgttgg ttgggttgaa gtctaatact aagaatgcag gcggattggc agagcctttt
    13441 ccaactgtcc aaatgccgtg atagccttgt tcttgtaatg cttcgccacg aatgatctgg
    13501 taagaaaccg tattcggttt tgccattttt tggataaact cgaccgcttg ttcggctaat
    13561 ttttcagggg ttaatttatc tgatggctta ttaattaatt cacgcacgaa agtggcacat
    13621 tttacacggt tatcaaattc ggcttgctgt tcgtctaagt ctgggtattg taccgagcct
    13681 tgatttttta ccgaatgaaa tccttgatga aacgcccagc aggattctaa atcccaacta
    13741 tcgccggtca gctccacggt tgaaatacct tgatttttaa ttttgcgggc ggctttttga
    13801 atagtggcta aatcgttgtt cggattatgt acggtaaacc ccgcatcatt tgcggaaaga
    13861 atagcatttt taccccaaat ttcaggggcg ttttgagtag aaagtgaaat tttcattgct
    13921 gtcattatcg gtttccttag aaaatggatt ttgctttatc ctaacaaact tttgcaaagt
    13981 tttatacaaa aatgaccgct tgcagagcat agccttgaca agcggtcatt gtttcgtgtt
    14041 tttacaacct gattaaggac ggattatttg ccgtcccaag ctttaattgc attgtggcgg
    14101 attttaacac ggctttcagc attgcctttg tagtaatctt tatctaaacc gccttcccag
    14161 tcgccatagt tcgggttcgg taacataatg aattttttac cgaattgttg gctgttttcg
    14221 gctacgaatg cacggcgttc cgcgtttgat ttacggtaag tcgcatcgcc gaaatcgttt
    14281 aagttgtcgc caatatagag cacgatttca tagccttgtt tttcgatttc ttcaaagcga
    14341 ggggttttgt tcgatttgtc ttttttcaac aagagtgttt gttcgctcac accactaaag
    14401 cctaatttgt tcatatcatc aatagtaccg gctttttcac tgctgtcttt acggtttgaa
    14461 acatagaaca tagttccttt gtggctatta acgtagttgt taaattccac tgcaccggca
    14521 atcgctccgg tttggcgggc atttacccaa cgagtccagc tttcaccatc aaacggcttg
    14581 tggtttttaa cctgccagcc ggcataaagg ctgttgtcca ccatagtttc atctaaatct
    14641 actaccacgg cttttttctt gccttttttc actttggctt ggtcgaatgc cactttcgct
    14701 gcattgaatg cttgatgggc aagagcttgg tattcgccgg attgttgcat ccagtttatc
    14761 cctaatgtag cttgctcttg caacaccatt tgatgatgct catcgctgtt tttatgcgtg
    14821 tgcgaacaag cagttaatac aaataaagct gtgaggctca tagcagaaag tttgatgttt
    14881 ttcatacgta tatccttagt tttattgttg ataaacagag cgattatacc taaaaagagt
    14941 tgcttatggc taatagttta ttgctcctat tcacttttaa agcaaactaa ggtagcctag
    15001 taaccttttt ataacaatta cacagaggct atatgcaaac attattaaaa tttacacaat
    15061 ttatcagcaa aaccttcgcc atttgggtgg tgttttttgc ttttatcgca gcacaatttc
    15121 cggaaacctt taagcaattc ctgccttgga ttccttatct gttagggatt gtgatgttgg
    15181 gaatggggtt aaccttaact ttcaaagatt ttgccgaggt aacgaaaaat ccgaaatcag
    15241 tgattatcgg cgtgatcgct caattcgtgg tatgccgagt gtagcttatg gattggcaaa
    15301 agcatttaac ttacccgcag atttagccat tggtgtgatt ttagttggtt gttgcccggg
    15361 cggtacttct tcaaacgtga tgacctattt ggctcgtggc aacacagcgt tatcggtagc
    15421 ttgtaccact atttcaactt tacttgcccc agtgctcacg ccggcaattt tctatttatt
    15481 tgccagccaa tggttagaaa ttaatgcaac ggcaatgttt atttcggtgt tgcaaatggt
    15541 gctattgcca atttttgtgg gcgtggtgat tcgtactatt ttcaaacaaa aagtagagca
    15601 atttagccaa acaatgccat taatttcagt gattgcaatc gtattaattg tcacggcggt
    15661 ggtgtcagtt agccgagatc gtattattga atccggttta ttaatttttg cggtagtagc
    15721 attacacaac ggtttaggtt atttaatcgg ttatttggcg agccgtttat taaaattacc
    15781 gattgcagac agcaaagcgg tggcgattga agtggggatg caaaactcag gattgggggc
    15841 agcacttgcc gcattacact ttaaagccaa ccctatcatt gcggtgccaa gtgcggtatt
    15901 tagcttctgg cacaatattt caggcccgat tttggcgatg attttcgcta aaatgaaaaa
    15961 cgataacgaa aaataaaatt cgttttaata cagacgtttt aatgttttaa taaagaatag
    16021 acaaacggtt aaatttttct aattttttgc aaaatgttca gaaaatttaa ccgcttgttg
    16081 tttagaattt agaacattct tggtccacgt gcagtgctga ttttttcatc atccgctctt
    16141 tgggtaacaa tcacttgatc accctcattt aagcctgcta atacttcggt attttgcgta
    16201 tccgaaatgc caatttggat ttttctttca accgactgct cattttccag caccaacaca
    16261 taattctctt taccacgttt tttgattacg ctggtgggta caactaaaac ctgttcccgt
    16321 ttatcaatag taatttgggc ttgagtagtc attccgatgc gtaaactgtt atcggcatta
    16381 tcaacggaaa cattagcgta gtaataaacc gcttcggtat tacccgattg ttcggtgtag
    16441 ttattatcgg tcagcgtggt gagtgccgga tcaactgaat caatggtagc actatattct
    16501 cgatttggct cagcaagggt ggaaaactta actttttgcc cgactttaac ctgtgcaata
    16561 tcgccctccg aaatttcaag tttaatcagc atcttagata aatctgctac ctgtacaata
    16621 gttggcgaag tttgattgga attgaccgtt tgcccgacag aaaccggtac agacacaatc
    16681 acgccgtcca ttggcgagat aatttgcgta taagttaagt tggttttagc atcgtttacc
    16741 gaaatttcag ccgattttaa ttgtgcttta atgttatcca ccgtggcttt ggctgaagct
    16801 aaggtatttt tagccgtttc aagatcgttt aaagaggcac tatttgcccg atagagttta
    16861 tttaagcggt tgtagttcga ttgagctact tcaagtgcaa cctgtgcaga ggtgagctgt
    16921 gctttataag aagaaagccg ggcttgagcg gtgcttaagg tgttgctctg gttgctggaa
    16981 tcaatatctg caattaaatc tccttgcttc accgattgcc cgagctcgac atagagtttt
    17041 tggattttgc ccgaaacttg agcccccact tcggtgcgtt tattcgctcg aaccgaaccg
    17101 gttgccagca catttttgtt aatttctgcc cgtgtgacag cttgggtaat gtaatggatt
    17161 tgcggtttat tggtttgttg ctgataataa aaatagccgc ctattccgag agcggcaatt
    17221 agcacaagag tggctaatgt tttacgtttc atactgttcc ttgatgatat acaagcggtc
    17281 aaatttagac aaaaatttgc aaaaaagttg ctctcaacta aacaatgcca aatgtggctc
    17341 gaagatagct tgccaccgct tcatctttgt taaagccaat cacttcatta ttcggtaata
    17401 cggcttttaa acgtggatcg gcattaccca ttaaacaacc tttgccggct aaacttagca
    17461 tttccacatc attcataccg tcaccaaaag cgatacaatc ggctaaggtg tagccccgta
    17521 atttgaccaa ttcggcgaga gcattggctt tacacactcc ttttgccatc acttcaaggc
    17581 aaagtagggc agagtaggtc atttgcacac ggtcgccata ggtatcccga atataatgtt
    17641 caatcggtac taaagcttca gcagttttgc cgataaaaaa cactttctcg gtatttcgac
    17701 cgtgatgatg agcaaaattt actacttgat agctaaatcc tgaatcttga tggaattttt
    17761 ttaactctgc aatctcttta ttgataaacc attcatcgcc ttgatagctg tttaagcaca
    17821 cattttggtc gtcaaattca atgttcatta attcaaaggc aagatcttcc ggtaaataat
    17881 ggttgagaat ttttttaccc tctaaattgt tggctcttgc cccgtttgaa gtgactaaca
    17941 ttgcattttt aacgtttact ttcccaataa tctgagacac atctggatga ttgcgaccgg
    18001 tggcgaaaaa aatatcaaca cctttttttg atagttgttc taatgtatca atagtgaaat
    18061 caccaatttt atgattggca tttaatagtg tgccatcaag atcggaaata attgcacgaa
    18121 atgg
//
LOCUS       DANLUA010000028        18013 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLUA010000028 DANLUA010000000
VERSION     DANLUA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18013
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            59..1150
                     /gene="ulaG"
                     /locus_tag="PXB44_000728"
     CDS             59..1150
                     /gene="ulaG"
                     /locus_tag="PXB44_000728"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL5281237.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1234..1980
                     /gene="ulaR"
                     /locus_tag="PXB44_000729"
     CDS             1234..1980
                     /gene="ulaR"
                     /locus_tag="PXB44_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL5281238.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2035..2895
                     /locus_tag="PXB44_000730"
     CDS             2035..2895
                     /locus_tag="PXB44_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL5281239.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2892..3611
                     /locus_tag="PXB44_000731"
     CDS             2892..3611
                     /locus_tag="PXB44_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL5281240.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3832..6309
                     /locus_tag="PXB44_000732"
     CDS             3832..6309
                     /locus_tag="PXB44_000732"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5281241.1"
                     /translation="MYLLEGNQKVKNRNYFVAIATILALSPEVNATELSDQLASRTRY
                     YPSATDSYYSYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTK
                     VYTTHNGRTTVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIV
                     DNKPITDTQPNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWND
                     RSRGYNIDDPNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTA
                     PASATHGIEVAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRG
                     ARIFNQSYGIPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRG
                     DLDPSLQAGLPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVT
                     AMGDYIFNLGGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGA
                     PGVDDVYGWGLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKN
                     GQGELILSGASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEG
                     TVVNQGYSTIKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQ
                     KGLSMSAITSDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSA
                     LDNQIEGNNGSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFEN
                     SETVNKDLSNRLVMLGTLDNVGETAGV"
     gene            6319..7134
                     /locus_tag="PXB44_000733"
     CDS             6319..7134
                     /locus_tag="PXB44_000733"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5281242.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSGHNNVVTAGIRINF"
     gene            complement(7221..8081)
                     /gene="pdxY"
                     /locus_tag="PXB44_000734"
     CDS             complement(7221..8081)
                     /gene="pdxY"
                     /locus_tag="PXB44_000734"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL5281243.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            8200..8275
                     /locus_tag="PXB44_000735"
     tRNA            8200..8275
                     /locus_tag="PXB44_000735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8233..8235,aa:Thr,seq:tgt)
     gene            complement(8370..9323)
                     /gene="accA"
                     /locus_tag="PXB44_000736"
     CDS             complement(8370..9323)
                     /gene="accA"
                     /locus_tag="PXB44_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL5281244.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(9377..10801)
                     /locus_tag="PXB44_000737"
     CDS             complement(9377..10801)
                     /locus_tag="PXB44_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL5281245.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(10803..11537)
                     /locus_tag="PXB44_000738"
     CDS             complement(10803..11537)
                     /locus_tag="PXB44_000738"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL5281246.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            11684..12181
                     /gene="tpx"
                     /locus_tag="PXB44_000739"
     CDS             11684..12181
                     /gene="tpx"
                     /locus_tag="PXB44_000739"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL5281247.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(12266..13891)
                     /gene="yidC"
                     /locus_tag="PXB44_000740"
     CDS             complement(12266..13891)
                     /gene="yidC"
                     /locus_tag="PXB44_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL5281248.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(14044..14469)
                     /locus_tag="PXB44_000741"
     CDS             complement(14044..14469)
                     /locus_tag="PXB44_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL5281249.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(14798..17890)
                     /locus_tag="PXB44_000742"
     CDS             complement(14798..17890)
                     /locus_tag="PXB44_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL5281250.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         5132 a         3639 c         3940 g         5302 t
ORIGIN      
        1 tgtttatgat tggttttgat tgcattgttc ttattttagc gtaaatcagg agactattat
       61 gagcaaaatt caagaaatca ctcgtgaaag ctggatcctt tcaactttcc ctgaatgggg
      121 tacttggttg aatgaagaaa ttgaagaaga agttgtaccg gaaggcaatt ttgcaatgtg
      181 gtggctaggc tgtgtcggtg tgtggattaa aaccccagct ggtgcgaata tctgtatgga
      241 tttatggtgc ggtcgtggta aatccaccaa gaaagtaaaa gatatggtgc gtggtcatca
      301 aatggcaaat atggcagggg tgcgtaagct ccagcctaat ttgcgggctc aaccgatggt
      361 gttagatccg tttgcgatta acgaagtgga cttcattctc gcttctcact atcactcaga
      421 ccatattgat gtaaacgtgg cagcggctat ttgcaataac ccgaaattag accacgttaa
      481 atttgtcggc ccttggcact gtgcagaatt atggaaaaaa tggggcgtgc cggaagaacg
      541 tattattgtg gtaaaaccgg gcgatgtcgt gaaattaaaa gatgtagaaa ttcacgccct
      601 agattcattt gaccgtacct gtttagtgac cttaccggta gaaggtgcag aagaacgtgg
      661 cggagaactt gcaggcttat gcccatcaga tgaagaaatg ggacgcaaag cggtgaacta
      721 tgtgttcaaa accccgggcg gtacgattta tcacggtgca gattctcact actcaatcca
      781 attcgctaaa cacggtaagc aatttgatat tgacgtagcg ctgaataact atggcgaaaa
      841 cccggttggt attgcggata aaatgacttc agttgattta ttacgtatgg cggagtgttt
      901 acgctgtaaa gtgattattc cggttcacca tgatatttgg acaaacttta tggcaagtac
      961 gcaagaaatt attgatttat ggcgtatgcg taaagatcgc ttacaatacc aattccaccc
     1021 atttatttgg gaagtgggcg gcaaatatgt ttacccacgt gataaagact taattgaata
     1081 tcatcaccct cgcggctttg atgactgctt cgagcaagag ccaaatattc aatttaaatc
     1141 catgctatag gtgagcagtc gccactctca ctggagagtg gcgttttagc aagttcatta
     1201 tggagcagta gtgattagta atgggataaa gtagtgaacg aaagttatag acacaaagag
     1261 ttattagccc tgttggaaga gcggaaagca ctgtcaacag cagatattat gcagctgtta
     1321 aatatttctc cggcaacagc aagacgtgat atcagtaaat tagatggtca aggtaaatta
     1381 aaaaaggtta gaaacggagc agaagcaatt ctggctccta catttttaca gcacacaaaa
     1441 ataactaaca atgcgcagga gaaacgtaga atagcggaag ctgccggtcg tttatgtaaa
     1501 gatggggaaa gtcttattct gacgtgtggt tccacaatgg aaatgctcgg tgaagtatta
     1561 tgtggtagaa aattgcaaat tattaccaat tatctaccgc ttgcaaacca gttgattgca
     1621 ggttgccacg atgatgtagt gattatgggt gggcaatata ataaacataa aaaagtgatg
     1681 ctctcaatta acacccaaaa tgagatgact tatgccgcta ccacaatgtt taccagtggc
     1741 aaaggcttaa ccagcgaagg attatttaaa accgatatga ttattgccaa tgccgagcaa
     1801 caaatggcaa agcacgcaga acgttatgtg gtgttattgg atagcaccaa gctcggcaaa
     1861 aaagtcggta tgctgtttaa agagttcgat aaaattgatt tattgattac cggcaaagag
     1921 gcggatccga ctattatcca agcattaaga aacaaaggcc tggatattat cttggcttag
     1981 cctgtagcgg tcaaatttaa tcaaaatttt acttatcatc ttcaagggaa aactatgaga
     2041 aaacataaac tgggtattta cgaaaaggcc ttgccgaaaa acatttcgtg gcaagaccgc
     2101 ctttctatcg ccaaagtctg cggttttgac tttgtggaaa tgtcgattga cgaaacggac
     2161 gaacgcttag cccgccttga ttggagcaaa aaagaacgtg ccaagctggt gaaaaccatc
     2221 atcaacaccg gcgtgaccat tccgtcaatg tgcttgtccg gtcatcgccg tttcccgttt
     2281 ggttcttgtg atgaagcgac ccgccaaaaa gcctatgaaa ttatggaaaa agccattcaa
     2341 ctggcggtcg atctcggtat tcgcaccatt cagcttgccg gctatgacgt ctattacgaa
     2401 gagcaggacg aaggcacaat ccaacgcttc caacaaggct tagaatgggc ggtggagctg
     2461 gcggcaagca accaagttac cattgcggtg gaaattatgg acacccaatt tatgagttcg
     2521 atttcccgct ggaaaaaatg ggacgagatc atcaaatcac cgtggttcac cgtgtatcca
     2581 gatttaggca acctctcagc gtggaacgac aacgtagcgg aagagctgaa actcggcatc
     2641 gacaaaatct ctgccattca cttaaaagac acttacaaag tgactgaaac ctgcaaaggg
     2701 cagttccgtg atgtgccatt tggtgacggt tgcgtagatt tcgccgcttg ttttaaaacc
     2761 cttgccgagc tgaattaccg tggcgcattc ttaattgaaa tgtggacgga aaaagccgaa
     2821 gagccgatta ctgaaatcat caacgcccgc cgttggatcg aacaaaaaat gcaagaagga
     2881 ggatttaccc tatgacgcaa ttaactgaat taacccctgc attacaagcc ttaagagagc
     2941 gtgtgttaaa agccaattta gaattaccga agtatggctt ggtaactttc acttggggta
     3001 acgtaagtga aatcgaccgt gagaccggtt tagtcgccat caaaccttcc ggcgtagaat
     3061 acgatgcgat gacggtggaa gacattgtat tggttgattt acaaggtaat cgagtttacg
     3121 gagataaaaa accgtcttct gatacagaaa cgcacttaga actttatcgc caattcactg
     3181 atattggcgg tgtggtacac actcactctc gcaatgctgt aggttgggca caagcagggc
     3241 gtgatttatt ggcacttggc accactcaag gcgattattt ctacggtgcg attccttgta
     3301 ctcgcaaaat gaccgcagag gaaattgccg gcagttatga gctggaaacc ggaaaggtga
     3361 ttgtcgaaac tttccgaact cgaggtattg aaccgaaagc agttccaggt gtgttggtac
     3421 attcacacgg tccatttacc tggggaaaag atggacacga tgctgtgcat aattctgtgg
     3481 tattagaaga ggtggcttac atgaatttta ttagccatca aattcgccca caaattggcc
     3541 caatgcaaca ggaattatta gataagcatt atctgcgtaa gcatggtgca aatgcttatt
     3601 atgggcaata gctaataacc taaaaattat tttccaaatt atcaacagct ttcgaggggc
     3661 tttctataat cggaatagct cctcagtctg tttgagttta tggatagatc cacaaataat
     3721 taccattttc cttttcttga ttttaatttt gatttttgtt ctaatctctg cctcttgttt
     3781 ttacaagtct attaaggatc cgtagtagat ttgaaataaa caagagtatc tttgtattta
     3841 ttagaaggaa accaaaaggt gaaaaataga aattattttg ttgcaatagc aacaatatta
     3901 gccttatcgc cagaggttaa cgcaactgaa ctttcagatc agcttgcaag tagaacaaga
     3961 tattatcctt ccgccacgga tagttattat agttactata aatatgcacc gatttattcg
     4021 ggctactata catcgaatag ccgtaatggt tattcgtcgt ggtattatcc aggtagcaat
     4081 gcaggtgtaa caaccagaac tgtttcgaca acagttaatg gcattacagt tacaaaagtt
     4141 tatacaacgc ataatgggag aacaaccgtt cgtaccactt atgctcctgc tgtgaacaaa
     4201 ccaattgtaa ataaaccgac accgattgcc aataaacccg ttgtagatac gccaactcaa
     4261 ccggttattg atgccaaccc aattatggat ccgccagtcc aaccaatagt tgataataaa
     4321 cccatcactg atacacagcc taatccaatt acagataaca aacctgtcat tgaatcgcca
     4381 attgatgaca cccgtccagt tatcattagg cctacaacac cggtaaatct tcatcctgaa
     4441 cctgtagctg ttggtgtacc caatgtaggt attcgatgga atgaccgctc tcgtggttac
     4501 aacattgatg atcctaataa caaacaaaat gttaagttaa ctggtgaagg tgttaaagtc
     4561 ggtatcttag atagtggttt taaaaactat ttaatgcagg acgatatact caaaaaattt
     4621 ggtaatcgcg ctgttattat tgaaacaggg cgtactgccc cggcttctgc aacacatggt
     4681 attgaggtcg cagaaatggt tgggggaggg aactctcgca atggagtagc gtctaagtca
     4741 acgttattac ttgcggatat taccaaaaca acaaatgcgg gtacaggatt atccgcaaca
     4801 gctccaattt attatgactt atggaatagg ggagcccgta tttttaacca atcatacggt
     4861 attccaaagc ctcttactta ttttaataac aatagaaatt ctagctatta ctatttacac
     4921 caatttgatg gtaatgtgtt gcaattctat aaggataaag taaaagaggg gggcttattt
     4981 gtctgggctg cggggaataa ccgtggtgat cttgatccat cactccaagc tgggttacct
     5041 cattatgaag ctgacttgga aaaaggttgg atttctgttg ttgcggtagc aaccagaact
     5101 aatcagggat tgggtaactt tgaatggagc aatttattgc catattctca agccggtgtg
     5161 gcaaaaaatt ggaccgttac cgcaatgggt gactacatct ttaatttagg aggtagaaac
     5221 attattgcta gtggttcatc ctttgcagct ccagccgtta cgggtacagc agctctcgtg
     5281 aaacaaaaat atccttggat ggatggtaac cttatcaaac aatctattct ctcgacggca
     5341 accgatatcg gcgctcctgg agttgatgat gtatatggat ggggtttact taatattgaa
     5401 aaagccacta atggccctgc gcgttttggt aaacgactcg ctttagccga taacgtgact
     5461 gtaaatattc caaatggaag ctatgagttt tccaataata ttaacggtga tgcaggcttg
     5521 gtgaaaaatg gccaaggcga attaatttta tcgggtgcta gtacttttga aggggataca
     5581 acactcaatg aaggtgctgt cactattaac ggtaaaaaat atgtgtctaa agtaaacgta
     5641 tctccaagtg gtacattggt tgtcaataat accacgttag aaaatggcgt aaataacgag
     5701 ggtacagttg tcaaccaggg ttattctacg attaagagag cttacgttgc ttcagcacaa
     5761 agtacgctag tgtcagattt aggttcaaac ttaaatgtaa aaggagaagt tgacttaaac
     5821 ggctctactt tatcactagc tgcggtaaaa gagggtaaag ctcagtatgt gacccaaaaa
     5881 ggtttatcaa tgagtgctat tacttctgat tccgcaatta agggtaactt tgctgcaata
     5941 gaaacctcag ggttattaac tgcgacggcg gaaaaaacca atgagaatga agtaaaagca
     6001 acagttagcc ggaaaaatgt cgcgactgct ttagagagct cattttcggc attggataac
     6061 caaattgaag gaaataatgg ttctacaaat gctacaaatg atgaattcgc acaacaagcg
     6121 gctactttac aaaacagtat tgcaaacatc aatacgcaaa gtgtggtttt agatagctta
     6181 tctggtcaaa tttatgcgtc tgcgcaagcg cttacttttg aaaactcaga aacagtgaat
     6241 aaagatctct ctaaccgtct tgtaatgtta ggaacgctgg ataatgtagg tgagactgct
     6301 ggtgtgtgag taacgggtat ttatgggaat ggtactctaa aagaaatagg gtttggtgaa
     6361 ggtaaaacca atacctatgc tggccaaata ggttttgata agccagttac agaccatgtg
     6421 attttaggtg cggcacttaa ttattctaaa gcaaaagtga acttcgatcg ttatggcggt
     6481 agttcaaaag cagatggaat aggtgcatca ttatatgccc gtataggcaa taagcataaa
     6541 acaccttggt atttacaagg tcgagccggt tatggcagtg ttgattcaga tgtagagcgt
     6601 catattattt tggcggataa taacgcaagt acagcaaaaa tcaaccaccg agacagagta
     6661 ttttctgctt atgttgaatc aggctatgac ttcaagcaag gtcgctttgc gttaactcca
     6721 tttgtcggtt ttagtcatga tgttgtcaga cgtggcgcat tcagtgagca aaacagtcaa
     6781 tttggtctca ccgcggacaa agcaacctat aaacaaacaa gcggattgat tggtttacga
     6841 acatctttag aggtgaattt agctgggatg aaaacgactt tccaaggcta tgtaaatcat
     6901 caaaaagcgt ttaatcgaga agatcttagc tttaaagcaa gttatacagg tttacaagat
     6961 gcaaaattcg acgtaaaagg tattgggctt gcaaaacata aaacgtgggt agggattggt
     7021 gcattggcgg aagtaaataa aaatacttcc ttatatgtga attacgacct taaactcgca
     7081 aaaaatagtg gtcacaacaa cgttgtgact gctgggatta gaattaattt ctaactgaat
     7141 ctttttaaac aaaacgggcg tttatacgcc cgttttttta tagttataga gataataatg
     7201 ttattttata cttcatttaa ttaggcaatc tttacagctt ggtaactaca ctgtggatta
     7261 acaatccgat cacgagaagc aataatttgt aactcataaa gccctgatct agcggtttgt
     7321 tccattacct cattgaccgc atttgcagtg tgttcaaagg cttctagatc ggattttcca
     7381 tttaataagt tcgccacaaa tagccctgcg gttaaatcgc ctacaccgac aggttctttg
     7441 gcaaattggt agagtgggcg gctgatgtgc caaatgccat cttggttggc gaacagcatt
     7501 tcaaatttgt tgctctcttt gccgactcgg cttaaatgtt taaccaatac ttttttcggg
     7561 cctttggtta aaatcacttt cactgcctcg accgcttgct caaaattttc caccgttaaa
     7621 ccggttaatt ctcttaattc cactaaattt ggtgtgataa tgtcggcttg tgccattgcc
     7681 agattgatca atccctcttt tacgccgtct gccacaatgc agcctttgtc ggggtgtccc
     7741 attaccgggt cgcatacata aattgcgttt ggattttccg ctttcacttt atgaaaagcc
     7801 tgaataattt cggctacttg ttctgccgag ccgatatagc cagagagtac ggcatcgcaa
     7861 cgatgcagtt caccgatatt gtgaatgcca tcaacaatgc taccgatttg ttcttttggc
     7921 atcaccatac cgccccattt tccatattgg gtatggttgg aaaactgtac ggtatttaac
     7981 gcccatacat caacacctaa cagctgcata ggaaaagttg aagccttatt gccggcatag
     8041 ccgtagacaa cgtgcgattg tatggatagt atgtttttca ttcattcact cctttaaaat
     8101 ttcaatatgt gttgtttttt agtcatttga agttgcaagg acttgaagct catcaagaaa
     8161 tctctataat cccgcctcgt tctcttatcc attcaatatg ccgacttagc tcagtaggta
     8221 gagcaactga cttgtaatca gtaggtcacc agttcgattc cggtagtcgg caccatcacc
     8281 tttattttct gatgagtccc ctttaatgta aaatggcaag attttgcaaa ttttcacaaa
     8341 aatcttaccg cttgtagggt gaaattgaat tagacgtaac cgtaattcat taagcgttga
     8401 taacggcgtt ccagtaaatc ctctttatta agttgatcta accctgccaa atcttcaaca
     8461 atacgctgtt taaggttttg ggcgatttgt tcaacattgc gatgagcacc gcctaagggt
     8521 tccggcacga tgttatcaat cagctcaagc tcttttaaac gaggggcagt taaacccatt
     8581 acttctgctg ccgttgaggc tttatcggca cttttccaca gaatcgaggc acagccttca
     8641 ggtgaaatta ccgagtaagt tgaatattgc aacatattga ccttatcgcc cacaccgatc
     8701 gctaatgcac cgcccgagcc accttcgcca attaccgtac agattactgg cacggaaagt
     8761 tgagacattt cacgcaggtt acgggcaatc gcttctgact gaccacgctc ttccgcacca
     8821 atgcccggat aagctcccgg tgtgtcgata aaggtgatga ttggcatatt aaagcgttct
     8881 gccatttcca ttaaacgtaa cgctttgcga tagccttcag gagcaggcat accaaagtta
     8941 cgtttcactt tctcttttac ggtacgacct ttctgatgac cgataaccat caccggacgt
     9001 ccgtccagac gggcaatacc accgacaatc gctttatcat cggcaaaggc acggtcgcct
     9061 gccagttcgt caaattcggt gaaaatatgc tcaatataat ctaaagtgta cgggcgattg
     9121 gggtggcgtg ccatacggga aatttgccac gcatctaaat tagcaaaggt tttcttggtt
     9181 aattcctcgc ttttcttttg taaacgcttg atttcatcat caagattaat tttgtcatct
     9241 tgctcgctga ctgctcttaa agactcgatc ttggcttcca attcagcaat cggcaattca
     9301 aaatctaaaa attcttggct cattctattt tcctttttat gggcaaaatt atggggcatt
     9361 atcgcttatc agtcggttat tgtccatttt caattacgct aaattgaccc atcgtacctc
     9421 tatcacgcag cataaaatcg ctcacgccaa aggtaaatgg cagttgctca ggggcagtat
     9481 tatcaaagcg aaccaacagc gtgatttctt gctccgcctc gagccaaacg gtgtcccgcc
     9541 acgcaagttg tttgataggg tattgcttgc ggtttagggt ttcaataatg aattttgcac
     9601 cttgtagcgt aaatccgacc gcttgtgtgc tatgtaaata ccaacgctca acgctgcctt
     9661 tttgcgcggt aaaatcaatg cgttttgggt caaaccgctt ttggttgatt aaataatcag
     9721 ccggacgaat ggcaaaacgt ctctcttggc tgattttcag gttaaattcc tctaagttga
     9781 aagccggtaa actcggttta ttattcaata ccgctgccat tccttcaggg cgaagctcta
     9841 aaatcacgtt atcattcaat tcgtcgttgt cagcaaagac ctgcccgatt ttgtaaaaaa
     9901 tatctctttt ttgaccgctt atcagcgata ccgctttccc ttcgcttaaa tcgaccaaca
     9961 cttccaccct ttcactcggt gcaagttgaa ccgattcggt ttcaagtggc tctgccagca
    10021 ttcctaagcc ggtggcgatt aggtacaacg gctgctcgtt atctaagcgt aattcataag
    10081 cacgggaaag tgaggcattg acgatccgca atctgaccca gccacgaggc acgttaaaat
    10141 aaggcgattc ctgcccgttg acaaataatc gcttaccgaa aaattgtgag gtgtttttat
    10201 ctaacacctg tacgccgtct ttgttgagca gctggtcttg cagaattaac ggaatgtcgt
    10261 tcacgccata tttattcggt aaagtggctt ttttgctctg ttcatcttca atcatccaca
    10321 agccggctaa gccacgatac acttggaatg ccgagttgag cattgtatcg gcgtgatacc
    10381 aacaagtaca agccggttga ttgatagaaa caatcggcga ccagctactt ttggggtcaa
    10441 gggaacgatg caccgtgcca atcatctcac ttgaggcttg caatccttga atattcattg
    10501 agattttttg cggtaaatta ttgagataag ttaatttgac aaaatccccc gattttaccc
    10561 gtacggttgg ggcaagatat tgcccgttca caccccatac atcaaccaat ttgcctttat
    10621 caaattgggt ttgtgcgggg cgtaaatcca accgaaccgg ctttcctcgc ccaatatcca
    10681 ttaaaggcgg aatgttcagt tgcggtcgct ctgctgctaa tagcggtgtt ggcactgttg
    10741 caaatccagc cgcaagggca gagccttgta aaaattggcg tcgggtgatc tgttttttca
    10801 tattaggcgt tttttgcagt gttttctaac tgttcgactt cttgattaag ttgttcaaat
    10861 tttgccaaca taatttgatg gcacttttcc atcagttctt tgacgttttc acgggaatag
    10921 cctgaagtat caatcggatc aagcatttca cagattactg tgccgttatt tgaacggttc
    10981 aaatccactt tattgtgtaa attagagcaa actaccggca caatcggcac gccggcagaa
    11041 atggctaaat ggaatgcacc ggttttaaat ggcagtaaac cacgcccacg gctacgagta
    11101 ccttccggga acatccacac cgacacattg cgttctttca cgatttgccc tgctttattc
    11161 attgtttcaa tcgcactgga acgcttttca cggtgaataa aaatattgcc ggttgcccag
    11221 taaaccaaac cgaaaaacgg aatccaaatc aggctttttt tgccgatact caccgtcttt
    11281 tctgccacca ttgaggaaat cgttaccata tcgtaattgt tttgatgatt gccgatatag
    11341 acaaccggat agttaatttc cggctttttg cgggtaatta attccaaccc gactaattta
    11401 tgcaaagcac caaaccaacg agctaccaca cccacgctac ttgggtgacg taaacgcagt
    11461 aacgcataaa tagtgccaaa aatagaaatg aaaatagcgg caagggcaac aattaaaata
    11521 cgaaaaattt ttaacataat ggaataacct taaaaataaa ttgtctagga gtatagcaaa
    11581 ataaggttat tttctaaagg ctatccacat taaattgata gtttcctatt ttgcaaaaaa
    11641 tcggtatatt taaaccgctt gtttatttga taaggagaaa aaaatgagta aagtgacttt
    11701 agcaggaagc ccgattgatg ttgcaggcac atttccacaa aaaggcgatg tggttgccga
    11761 ttttacctta gtcaacagtg atttaagtga tgtttcgctt tccgattttg atggcaaacg
    11821 caaagtgtta aatattttcc caagtattga caccggcatt tgtgccactt ctgtgcgtgt
    11881 gtttaatcaa aaagcggctg atgtagcgaa tacggttgtc ctttgtattt cagcggattt
    11941 accttttgcc caagcacgtt tctgtggagc agaaggaatt gaaaatgcca aagtattatc
    12001 cagtttccgt catcgtgatt tacacaacaa actgggggta gatattattt caggtccgtt
    12061 ggcaggctta acttcacgtt cagtaattgt gttagatgaa aacaacaagg tattacacag
    12121 cgaattagtg ccggaaatta aacaagagcc ggattatgag gcggcgttag cggtattata
    12181 atttgcagaa ttatgctgga aaataaccgc ttgtaaagca acaaaaagcc cgttgattca
    12241 attcaacggg ctttgttttt agtgattatt tcttacgaga gtgtaagcct tttttctcaa
    12301 ggttacggta aatcaaccat tgctgtgcaa tcgtgattaa gtttgaggtt aaccagtaaa
    12361 gcactaagcc tgatgggaac cataagaaga acacggtaaa gataaccggc ataaaggtca
    12421 ttactttttg ttgtaccggg tcagctaccg gtgttggcga cattttttgt aacaagaaca
    12481 tcgaaccacc cattaataaa ggcaaaatgt agtatgggtc ttgtgccgat aagtcttgaa
    12541 tccagccgaa gaaaggtgcg tggcgtaatt ctaccgcttc cataaatgtc caatataaag
    12601 caatgaaaat cggcatttgg attaaaattg gtaagcagcc acccattggg ttcacttttt
    12661 cttctctgta gagtttcatc atctcttggc tcatacgttg gcggtcgtcg ccgaaacgtt
    12721 cacgcatttc agtaatgcga ggttgtaaca tacgcatttt tgccatcgag gtgtattgtg
    12781 ctttggttaa tgggtaaaga atggttttca ctacaatagt gacaccgata attgccaagc
    12841 cccagttgct tacgattttt tgaattgcgg ttagtaacca gaaaagcggt ttagcgataa
    12901 accacgccca gccgtaatct acggttaagt ctaggtgatt tgctgattcg cccatttctt
    12961 tctgatcttt agaacctgtc caaagtttgc tatttaaatt agcctctgaa tttggcgcaa
    13021 tcgtagttaa agggccacgg taaccgatag ttgctacccc attattggtg cgtgaataaa
    13081 ggctgttatc cgcatcttgg tttggcaccc aagcagacac gaaatagtgc tgtaataccg
    13141 caatccaacc cgctttggtg tccacgctta agttggcttt ttccatatct tggaaactgt
    13201 attttttgta gttggtttct gaagaagaat aagccccgcc ggtgtaagtc ggcatggtta
    13261 aattgccgga actttcaact atagagtgtt ttaattgccc gtaaggctga acttcaatcg
    13321 cttgtgcagt attatttttg atattgaagt ttaccccaac atcgtagcta ccacgtttta
    13381 gcgtgaaagt tttggtgtaa accacaccct ctttttcaaa ggttaatggt acagaaattt
    13441 cattttgccc ttcggctaat ttgaagctat ctgccgttac cgcatattgt gggcgaccgg
    13501 cattggtatc aatcccgttt ttacccacta aaccgctttg agcaacgtag attttgtcgc
    13561 cgtcttgctg taatagttta aatggcgtgt tagagtgtaa ttcagcattg tgttttaaca
    13621 aatctgagcc gatgacatca ccgcctaagg tatcaatagt cagacgtaat acatcgcttt
    13681 ccacggtaat ggttttacct tgcgtgatgc tatctgcgat tggattattg gcattggaag
    13741 cagacggaag atcacccgct tgttgggttt gtgctgccac ttgagcttgt ttttgtgctt
    13801 gaatttcagg atcaaaatct tgttgccatt gtgtaaaaat caaaatagac acaagaaata
    13861 aacccattag cagtaagcca cgattattca tgttggaatt ctcttgttag taatggataa
    13921 aaagaaaggt attttataat aggttagctt gttttccaaa cgccttttat gaacaagcgg
    13981 tcggattttc caagtttttt gcaaaatttt cggagaattt gaccgcttgt ggagaataag
    14041 aaactagcgt ttatctaagg ttttggtgga aaggcgagca actgcacaca ggttaccttg
    14101 ttcgtcttta atgtcgatat tccaaacctg tacttcacgc cctaatttca gcggtgtagc
    14161 tttggcaatc gcttttgtgc ctgccggaat cgattttaaa tggctgatgt ttagctccat
    14221 tcccaccgca atttgatggg gttcacacac cattaacgcc cctgcattgg cagtagtttc
    14281 cgcgagtgct gccgatacac cgccgtgcaa tacgccaaac ggctgttgag tgcgttcgtc
    14341 cacctgaagc tgtgcttcaa tccaatcttc accgatagcg gtaaattcaa tccctaagtg
    14401 tgacaccgcc gatttttggc tgagtgtgtt taattgttca caagttgctg tttgcttcca
    14461 aatggtcatt attatttcct gtctaataaa aaagctgtct atttagacag ctttcattat
    14521 ctctttgagc gaaacatact acgccaatag tacattgttt ggtcgcccat attaccttaa
    14581 gccatatagc actaaggagg tgatttatgt gagaaaatca gaagttttct ttctggttgc
    14641 gttgctaata ttattagcaa cttccgctag cattaaagta ctcaccatac ttgatgtgct
    14701 agtttggtta ttcgctagat aacctaaaat cagacagagg caggctggtt acctgcctcg
    14761 ctttttattc taagtacttt taggttacat ttcaacctta aatcacgtcc agcaacgctt
    14821 gaattgggtg tttaatgcct tgttgttcaa aacgtttgac ttgcgagcgg caggagtagc
    14881 cggttgctag gcaacgctca agcggtttat tttttaattt aatttgccat gattgctgat
    14941 aaatcgcttt cgacatttca acgtgtttgg tttcgtgtcc gaacgtgcct gccattccac
    15001 agcaaccgac attttcattg agtaaggttt caccaaaatg ggtgaaaata gcttgccact
    15061 ctttcattga gcttggcaag gcggtggatt cggtgcagtg agcaaataaa taccacggca
    15121 agcggtcgga atttgccgat tttttgcaat tttccagctc gccgttttta atcacttccc
    15181 ttaaccattc gtgtgccaat aagactttga aatccccccg agcttctttg aggatctctt
    15241 tgtattcttc acgataaagc aaagttaagg caggatcgac agccaccatc ggtagcccca
    15301 atttcgccac acggtttaaa aaatcggctt ggtttttcgc tgttttcgca aatttatgca
    15361 aaaagccttt aacgtgttgt gctttaccgc ttggcttgaa tggcaacaca atcggcttaa
    15421 agcctaattt tttaatgaga gcgataaagt ctgccaccac tttggcatcg taataagagg
    15481 taaacggatc ttgcacaata aacaaggttt tgcaattttt tgcagaaaaa tcaccgcttg
    15541 tagcttgttg ttcaaggctt tctaagctct cgccttggta gccgacttcc accaactgat
    15601 gttgcagatt tggcactgac aatgccggca aataggtcat tccaatggtt ttgtttgcag
    15661 ctttttcggc aaaagttgag gtgctgaaaa agttaaaaaa acgtggttgt ttcgccatta
    15721 aaggggcgat aaattctaag tttgagacca cataatcttt cagtggtcgc aaatagcggc
    15781 tgtggtaaag ctcgttaaat tgcgatctga acgtcggtac gtcaattttg attgggcatt
    15841 ggctggcaca ggctttgcag gcaaggcagg tgtccatcgc cgctttgact tcgtgtgaaa
    15901 aatcatactc tttgttttta ttgagcgtgt tgcgaatttt tgcaacaaaa tcagctaatt
    15961 tgaccgcttg tttgcgaggt ttgaagatca aatcgtccgg cgaaacgttt tgttcggtaa
    16021 gtaatctgag ccattcacgc acaagagtag cccgcccttt cggtgagtaa agacggttgc
    16081 ccgagacttt cattgaaggg cacatcgtgc tgtgtacatc aaagttaaaa catagcccgt
    16141 tgccgttaca attcattgct cctttaaatt cttctcgcac ttggattgga atttggcggt
    16201 caaaatcggc tcgcatttgt gagtcaatcg ggtagagcga ggtttcgctt tctaatggtg
    16261 tacaaatttt acctgggttc aagcgattgt acgggtcaaa taaggttttg atgtaacgga
    16321 gttcttgcca caaagtttcg ccaaagaaac gttcgccgta gtaagaacgc atacctttgc
    16381 cgtgttcgcc ccaaatcaag ccaccgtatt tggcggtgag ttcgactact tggtcggaaa
    16441 tttgcttaaa ggtttccact tgggctttgt cgcataagtc aagagcaggg cgaacgtgta
    16501 gcacgccggc atcaacgtgg ccgaacattc cgtaaggcag attatagcga tctaataaag
    16561 ctcgaaattc cacgatgtaa tcggcaaggt tttccggtgg cacagcggtg tcttctacaa
    16621 aggcgatcgg ttttgcccag cctttggcat tgcctagcag gcctacggct tttttccgca
    16681 ttgcgtagat tttctcgata gaatttacat cactcgtgag ttggtagccg ataatcccgg
    16741 cttcattatt tgccattttt tgatcaaggc tttggcagag tgctttcact tgggtttcaa
    16801 tttgagtctg atcgcttgag gcatattcaa caatgttaat ccctaaaatc gggttggctg
    16861 ggtcttcggt taataaatct gacaccgaat gccagacgat atcttgcttg gcaagattga
    16921 gcactttgga atcaaccgtt tccaccgata aggcttttgc ctctaacata aaaggagcgg
    16981 agcgtaaggc ggcatcaaag gaattatatt taatattaat taaggtgcga tatttgggaa
    17041 tcggcagtaa attcagtttg gcttcgcaaa taaaggcaag cgaaccttca gaaccggtta
    17101 aaatacgggt aaggttaaat tcgctttcac catggttaaa cacatttttt aaatcatagc
    17161 cggtaataaa acggttaagc tgaggcagtt cgttcagcac tttggggcgt aatgttttgc
    17221 aacggttgaa aatttcacgg tgcaaggttt tgccgagagc ggagagattt aacccttcaa
    17281 tatgtgcaaa aaaatcggca gatttgaccg cttgtgtctc taaaatatcg ccgttaataa
    17341 gcacagattt gatggcaaga atatgatcag atgttttgcc atattgcaaa gaaccttgcc
    17401 cagaggcatc ggtgttgatc attccgccta aagtcgctcg gttactggta gaaagttcgg
    17461 gggaaaagaa aagtccatgt ggttttaaaa attgattgag ctggtctttc accacgccag
    17521 cctgcactcg aacccaacgc tcttccacat tcagttccaa aatctgattc atatggcggg
    17581 agagatcaac aataatatta ttgttaagag attgcccgtt agtgcctgtg ccaccgcctc
    17641 gtggagtaaa agttaaatgt tgatattctg gcttttgggc aagtttggtt agaattacga
    17701 catcagaaac ggatttcgga aacagaatag cttgcggaat ttgctgatac acgctgttgt
    17761 ctgttgccag tgaaagccta tctgcataat ttgatgcaat ttccccacta aaatgctgat
    17821 tgacgagttc agttaaatag gattgggtgg ctttatccag ttgcggtact tcagagagtc
    17881 taggtatcat aagctgtgtt tggtttttta taataaaagc actaaatttt gcttatctta
    17941 gcaatttttc taaaaattgg gaatggaata tcaatttgat taaatagtgt tcgaaaaatg
    18001 gttttttggt taa
//
LOCUS       DANLUA010000029        17564 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLUA010000029 DANLUA010000000
VERSION     DANLUA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            74..1207
                     /gene="carA"
                     /locus_tag="PXB44_000743"
     CDS             74..1207
                     /gene="carA"
                     /locus_tag="PXB44_000743"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL5281251.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1310..4516
                     /gene="carB"
                     /locus_tag="PXB44_000744"
     CDS             1310..4516
                     /gene="carB"
                     /locus_tag="PXB44_000744"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL5281252.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4591..5382)
                     /locus_tag="PXB44_000745"
                     /pseudo
     CDS             complement(4591..5382)
                     /locus_tag="PXB44_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            5585..6583
                     /gene="yiaK"
                     /locus_tag="PXB44_000746"
     CDS             5585..6583
                     /gene="yiaK"
                     /locus_tag="PXB44_000746"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL5281253.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            6708..7073
                     /locus_tag="PXB44_000747"
     CDS             6708..7073
                     /locus_tag="PXB44_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5281254.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            7137..7616
                     /locus_tag="PXB44_000748"
     CDS             7137..7616
                     /locus_tag="PXB44_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5281255.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            7617..8894
                     /locus_tag="PXB44_000749"
     CDS             7617..8894
                     /locus_tag="PXB44_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL5281256.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            8997..9983
                     /locus_tag="PXB44_000750"
     CDS             8997..9983
                     /locus_tag="PXB44_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5281257.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            10057..11040
                     /locus_tag="PXB44_000751"
     CDS             10057..11040
                     /locus_tag="PXB44_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5281258.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            11171..12655
                     /locus_tag="PXB44_000752"
     CDS             11171..12655
                     /locus_tag="PXB44_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5281259.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            12850..13896
                     /locus_tag="PXB44_000753"
     CDS             12850..13896
                     /locus_tag="PXB44_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281260.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            13909..15018
                     /locus_tag="PXB44_000754"
     CDS             13909..15018
                     /locus_tag="PXB44_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5281261.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            15123..16013
                     /locus_tag="PXB44_000755"
     CDS             15123..16013
                     /locus_tag="PXB44_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL5281262.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIAILPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            16085..17554
                     /locus_tag="PXB44_000756"
     CDS             16085..17554
                     /locus_tag="PXB44_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL5281263.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
BASE COUNT         4916 a         3855 c         4136 g         4657 t
ORIGIN      
        1 cgtttgcttg attaaactta gtcgcctact tctaaggcta cacagataaa atttgatgat
       61 taggagtaaa acaatgttca aaccagcaat tctggttctc actgacggtt ctgtcttttc
      121 cggcaaatcc attggtgctg acggtcacac tatcggtgaa gtggtattta acactgcgat
      181 gaccggttat caagaaatcc ttaccgatcc ttcttatact aaacaaatag tcaccctgac
      241 ttacccccac attggcaata ccggcattaa tttagaagat aaagaatccc ccaatgtttg
      301 tgcctccggc ttaattattc gtgatcttcc tcttctccat agcaacttcc gtgcgtcctc
      361 ctcactttca gattatctca ttcaacaaaa cattgtcgct attgccgata ttgatacccg
      421 ccgtttaacc cgaattttac gcgaaaaagg ggcacaagcc ggttgtattt tagtaggcga
      481 tgccacagaa gagaatattg ctaacgcttt ggcatttgcc aacagtttcg gttctatggc
      541 tgggaaagat ttggctcaaa gtgtcacctg caatgaatta tataaatgga cagatggtga
      601 atggcaatta ggtaaaggtt atgtaccgca acaacaaacc gagttccacg tagttgccta
      661 tgattacggt gtaaaacaca acattttgcg aatgcttgcc gaacgaggct gtcgcttaac
      721 cgttgtgccg gcaaaaacct ctgctgaagc tgtgcttgcg ttaaatccgg acggtatttt
      781 cctttctaac ggcccggggg acccggagcc gtgcgattat gccattaccg caatccgaaa
      841 attattggaa acgcaaaaac ccattttcgg catttgctta gggcatcaac ttttaggttt
      901 agcggcaggc ggtaaaacca aaaaaatggt gtttggtcat cacggagcta atcacccggt
      961 acaagattta gacagccaaa aagtgctcat caccagccaa aaccacggtt ttgaagtaga
     1021 tgaatccagc ttaccatcta atgtgcgagt gactcaccgc tcactgtttg acggcacggt
     1081 gcaggggatt gaactcactg atagaccggc gttttcgttc caaggtcacc cggaagccag
     1141 cccggggccg aatgatgttg cctatttatt tgaccgattt gtcagtgaga tgaaaaaagt
     1201 gaagtaagga cgtacctatg tgtgtccatc aagcggtcga aatttgcaaa aaacttacca
     1261 aaaatgaccg cttgtagcct gaaataaacg agataaaaga gagcccaaaa tgccaaaaag
     1321 aacagacata aacactatcc taattatcgg tgccggtccg attgtgatcg gtcaagcgtg
     1381 tgaatttgac tactccggag cccaagcgtg caaagcgttg cgtgaagaag ggtataaagt
     1441 ggtgttggtg aactccaacc ctgcaaccat tatgaccgac ccgaatatgg cagatgtgac
     1501 ttacattgag ccgattgaat ggaaaacggt tgaaaaaatc attgaaaaag aacgccctga
     1561 tgccatttta ccgactatgg gcgggcagac agcgttgaac tgtgccttag atctttcgaa
     1621 aaacggtgtg ctgaaaaaat ataatgtaga gctgatcgga gctaccgaag acgcgattga
     1681 taaagccgaa gatcgtggtc gttttaaaga agcaatgact aaaatcggct tgaatacgcc
     1741 aaaatcattc gtttgccaca gttttgaaga ggccttagca gcacaagagc aggttggctt
     1801 tccaaccctg attcgcccct ctttcacgat gggaggctcc ggcggtggta ttgcttataa
     1861 tcgagatgaa tttatcgcca tctgcgaacg tggttttgaa gcctcgccta ctcacgaatt
     1921 actgattgaa caatccgtac taggctggaa agagtatgaa atggaagttg tgcgtgaccg
     1981 tgccgataac tgcattatcg tctgctcgat tgaaaacttc gacccgatgg gtgtacacac
     2041 cggcgactcc attaccgttg ctccggcaca aacgctaacc gataaagagt atcaaattat
     2101 gcgtaacgct tcgctggcgg tattgcgtga gatcggcgtg gacaccggcg gttcaaatgt
     2161 gcaatttgcg attaaccctg caaacggcga gatgatcgtc attgaaatga accctcgtgt
     2221 gagccgttct tctgcactcg catctaaagc caccggcttc ccgattgcca aagttgcggc
     2281 gaaattggct gtaggctaca cactcaacga attgcgtaac gacatcaccg gagggctgat
     2341 tccggcttca ttcgagccga ctttagatta tgttgtgacc aaaattccac gcttcgcctt
     2401 tgaaaaattt gccaatgcag acgaccgatt aaccactcaa atgaaatcgg taggcgaagt
     2461 aatggcaatg ggaagaacct tccaagagag tttacaaaaa gccttgcgag gcttggaaac
     2521 cggcatttgc ggctttaact tgctttccga agagcctgaa aaaatccgcc gtgaacttgc
     2581 caaccccgga ccaaaccgta ttttgtatgt tgccgatgcc tttggagcag gcttttcatt
     2641 ggaagaagtt caccactatt ctaaaatcga cccgtggttc ttggtgcaaa ttcaagactt
     2701 agtacttgaa gaattagcgt tagagaaaaa acagtttagc gatctagatg ctaacgaaat
     2761 ccgccgctta aaacgcaaag gcttctcaga caaacgtatc gcacagctta ccaaagtcag
     2821 cgaaaaaatg gtgcgggatt accgccatac ttacgctatt ctaccggtgt ataaacgggt
     2881 agatacctgt gcagccgagt ttgcctcaga tacggcttac ctctattcta cttacgagga
     2941 agagtgtgaa gccaaaccgt ctgatcgcaa aaaagtgatg attttaggcg gcggaccaaa
     3001 ccgtatcggg caggggattg aatttgacta ctgctgcgtt cacgcctcgc ttgctctgcg
     3061 tgaggcaggt tatgaaacca ttatggtgaa ctgcaaccct gaaaccgttt ccaccgattt
     3121 cgacacctcc gaccgcctct atttcgagcc gctaacatta gaagatgtac tggaaattgt
     3181 gcgtgtagaa aacccgtggg gcgtgattgt gcattacggc ggtcaaacac cactgaaatt
     3241 agcgaatgcc ttatctgatg ccggtgtaaa cattatcggg acttccgccg acagcattga
     3301 tgccgccgaa gaccgtgaac gtttccaaca aattctaaac actctcggtt taaaacagcc
     3361 ggcaaatcgc accgcccgta atgcgttgga agcggtggca ttggcaaatg aagtaggcta
     3421 tccgctggtg gttcgcccat cttatgtgct aggtggtcgg gcaatgcaaa ttgtgtataa
     3481 cgatgaagag ctaaaccgct atatgcgtga agcggtatcg gtttccaacg acagcccgat
     3541 tttactcgac cacttcctca ataatgcgat tgaagttgat gtggattgca tttgtgacgg
     3601 cgaaaatgta ctcattggcg gcattatgca acacgttgag caagccggta ttcacagtgg
     3661 cgatagtgcc tgctcactac ctgcctattc gttaagcggt catgttttgg acgaaattcg
     3721 caaacaaacc aacgcaatgg cgtttgctct cggtgtgaaa ggcttgatga acgtacaatt
     3781 tgcagtacaa aatgaggtcg tttatgtgtt ggaagttaac cctcgagcca gccgtactgt
     3841 accgtttgtc agcaaagcta ccggtcgccc actggcaaaa atcgctgctc gcgtgatggc
     3901 tggtgaaacc cttgccgact taaatgcatt agaggaaatt atcccaagca aattcttcgt
     3961 taaagaagcg gtattcccat tcatcaaatt cccgggtgtg gataccattc tcgggcctga
     4021 aatgcgttct accggcgaag tgatgggcat aggtaatacc tttgctgaag cgttcttcaa
     4081 agcacaactg ggtgccggtg aacgaattcc aaaagtcggc aaagtgttta tgtcggttat
     4141 tgaagagcat aaacctgctt taatttacat tgccaaacgt ttccaagaac aaggctacgg
     4201 tttatgtgcc accttcggca ctgcaaactt cctacggcaa cacggaattg cagtccaaac
     4261 ggtaaacaaa gttcgtgaag ggcgaccgca tattgtcgat gcgatcaaaa acggcgaaat
     4321 tgcgttggta atcaacaccg tcagcggcga gcctgaagtg attgccgaca gccactctat
     4381 ccgccgtacc gccttgcaac aacgtgtgcc tatttacacc acccttgcag gtgctgatgc
     4441 tatctcctac ggcgtaaaac atatcaatga gtttgatgtg tattcagtgc aggaattaga
     4501 acaaaaggct aactaaaata aagctaaaat gacaagcggt cagattttgc aaatttttta
     4561 caaattctga ccgcttgtgt ttgtagagga ttatttagta aatttatatg ctaaatcatt
     4621 ccagcgtaat tcatttttga gttgtggtag ctcggtattt tggttgatat ggataaactc
     4681 aataccaaaa tattcagcga acattcgtag catatcggca tcaatatcta aactaaatac
     4741 actatgatga gcaccgccgg ctagaatcca tgcttgagta cctacataaa aatttggttg
     4801 aagcttccaa aaagcatgcc caactggtaa atttggcatt ggatttggtg aggcgatagc
     4861 gtctaaatcc gcaacaacca ttctaaaacg gtctcccata tctacaacgg ttgcattcac
     4921 tgcttttccc gccttactac tgaaaattaa gcgaacagga tctgccttac cgccaatacc
     4981 tagcggtcga atggctaatg ttgatttctc tttggcaata gacggacaaa cttcaagcat
     5041 atgagccccc aataccactt ggttttgttc atctaaatca taggtgtagt cttccataaa
     5101 ggaagaaccg tttggtaagc cataacccat cactttcact tcacgcacaa gagctgcggt
     5161 tttccaacct ccttcaccac caaatccgta gccttgttcc attaaacgct gaacagccaa
     5221 gcccggtaat tgtttaatcc catttagatt ttggaaatta tcagtaaaag ctttaaatcc
     5281 accattatct aagaatgttt ttaagccgat ttcaatacga gcagaatatg cgagagattc
     5341 tcttgctgaa ccattttctt gcaagctatc ctcaacatta tacaagcggt cagattttgc
     5401 aaaaaaatga ccgcttgttg ccatttctaa agtgtgatct acctctcaaa tttacaaaat
     5461 tttttataac atattgattt aaaatgtttt ttatattttt cctttaattg actttaattt
     5521 gtcttaagag taacttataa aacgtgattt tactatttga aatattgagg tattcaagat
     5581 gactattcga gtttcctatg aagaattaaa agctcaattt aaacgtgttc tgttggctcg
     5641 caatgtgcgt gaagagattg ccgaagagtg tgccactgtg tttgccgaca ccactcaagc
     5701 gggtgcttat tcacacgggg taaaccgttt cccacgcttt attcagcagt tagagaacgg
     5761 tgatattgtg ccggaagccg tgccgactaa agtgctttca ctgggggcga ttgagcagtg
     5821 ggacgctcac caagccatcg gtaacttaac cgcaaaaaaa atgatggaca gagcaatgga
     5881 gcttgcatct gaaaacggca tcggcgtagt ggcgttacgt aatgcaaacc actggatgcg
     5941 tggcggttct tacggatggc aggcggcaga gaaaggctat atcgggatct gctggacgaa
     6001 cgcccttgcg gtaatgccgc cttggggggc gaaagagtgc cgtatcggga ctaacccgtt
     6061 aatcattgcg gtgccgacta acccaatcac gatggtggat atgtcttgct caatgtactc
     6121 atacggaatg ttggaagtgc atcgccttgc tggtcgtcaa acctttgtcg atgctggttt
     6181 tgatgatgac ggcaacccaa cccgtgatcc ggcaacggtc gagaaaaacc tccgtctaat
     6241 gccgatgggc ttctggaaag gttcgggctt atcgattgtg ttagatatga tcgccacctt
     6301 gctctctaac ggtgaatcca ctgcggcagt caccgaagat aaagacgatg aatattgcgt
     6361 gtcgcaagtt tatatcgcta ttgaagtgga tcgcttaatt gacggcaaaa ccaaagacga
     6421 aaaactcaac cgtattatgg aatatgtgaa aaccgcagaa cgtgccgacc ctgatgtggc
     6481 agtacgttta ccgggccacg agttcaccca aattctagcc gataacaaag ccaacggcat
     6541 tccggtggat gacaccgttt gggcgaaatt acaggcgttg taattgcaaa ttgaccggta
     6601 gggtgggcgt aagcccacgc attagacaca atcataagat gacgtgggct tacgcccacc
     6661 ctacaagcgg tcatttttcc gcagaatttt acaaaacgga ggtatgaatg ttttttggtc
     6721 atatttcaca agttaaagcg aatcgatacc cgccggcgat ccaaattgcg ttgaactatc
     6781 tgaaaaatac cgattttgat gcgatggaag caggggttta cgaactcaaa ggcagacaaa
     6841 tttatgtgca ggtgttggat ttgaacacca aatcgaaaca cgaatttcag ccggaagtgc
     6901 atcgcaatta cctcgatgtg cagtatctac accgaggcaa agagataatg gctgcggcgg
     6961 tggataccgg caccaatccg attgcgatgg aatacaaccc tgagcgggat attcaatatt
     7021 atcaatccgt tgccaacgaa aatgaatttc gctgcgttga aggcaatttt tgacggcacg
     7081 gaaaccattc gcaaagtggt ggtaaaaatt gcgatgagcg aaattgagga gcaacgatga
     7141 aagccttagc tcaattagtc ggaaaactgt tagaaatctt ggtggtggtg attttatccg
     7201 caatgtcgat tttggttttc gtcaatgtgg tactacgtta cggttttaac agcagcatta
     7261 gcgtgaccga agaggtgtct cgctatatgt ttgtttggtt ggcgttttta ggggcggtgt
     7321 tggcatttag cgaaaatcaa cacgttaaag tcacaatgct gaccgataaa ttatcgccaa
     7381 cggctcgtaa tatcttaagc gtagtcacag atttagcgat gttgttctgt tgctacttaa
     7441 ttgtggacgg tagctggatt caatttaacc tcaacctgaa taactttgcc ccgatttcag
     7501 gcttaccgca aggcatcact ttccttgcca gcacagtggc aggggtatta atcggtttat
     7561 taattattgc ccgcattgtg gcaacggttg gcgtgattgc aaaaggagaa aaatagatga
     7621 ctgtggtaat ttttctttcc gtcttgcttg gctcgatttt attgggcatt ccggtagctt
     7681 tcgccctgtt ggtctgcggt gtggcgttaa tgttgcattt agatctgttc gatgcccaaa
     7741 ttttggctca acaaattgtg agcggtgcag acagtttctc gctaatggca attccgttct
     7801 tcattcttgc cggtgaaatt atgaacgagg gcggtctctc caaacggatt atcgacctgc
     7861 cgatgaaatt agtcggtcat aaacggggcg gtttaggttt tgtagcaatt ttagcggcga
     7921 tgattatggc aagcctttcc ggttcggcgg tggcggatac cgcagcggta gtcgcaatgc
     7981 tactgccgat gatgaaaacc accggttatc cgattgaccg ctctgccggt ttaatcggta
     8041 cggcagggat tatcgcccca atcattccgc caagcattcc gtttatcgta tttggtgtgg
     8101 ctagtggcgt gtccattacc aaactgttct tagccggtat tttcccgggc attatgatgg
     8161 ggatctgctt ggctgccctt tggtggtggc aggcaaaacg tttggattta atgaccttct
     8221 ccaaagcgac caaagaagaa ctttgcattt catttaaaaa cagtatttgg gcgttattgt
     8281 tgcctgtcat tattatcggt ggtttccgct cgggaatgtt caccccaacc gaagcgggtg
     8341 cggtcgcaac tttctatgcc ttgattgttt cactgtttgt ttaccgtgaa atgaagttca
     8401 aagatttata caaggtagtg ttggcggcag cgaaaaccac ggcagtagtg atgttcttag
     8461 tggcagcggc aaatgtgacc ggctggctga ttaccgtagc ggaattaccg atgatgctca
     8521 ccgagctgtt agagccgtta attgaaagcc cgaccacact cttattggtg attatgttag
     8581 cggtgtttat tatcggaatg gtgatggact taaccccaac cgtgctgatt ttaacgccgg
     8641 tattaatgcc gttggtggaa gaggcaggca ttgacccggt ttacttcggt gtgctgttta
     8701 ttcttaacac ctccatcggc ttgattacac caccggtcgg caacgtgcta aacgtgatta
     8761 ccggcgtgtc gaaactgccg ttcgaccaag cggctaaggg cgtaatgcct tacctcgtaa
     8821 tgatgatttg tttactactg ttgttcgtgt tcttcccgtc actggtgtta gtgccgttaa
     8881 gctggatgca gtaatggttt gaaccgaaac aagcggtcga attgacgcaa aaatttgcaa
     8941 atttttttgg caaaacagac cgcttgcaac tcaattttta atcacaggag aaccctatga
     9001 aattatttaa tttaaaaacc ttatttgcct tagtggcagg cgttgcggta atgagcggct
     9061 ctgcaatggc agaaacctca ctgcgttttg gttatgaagc cccacgttcc gatacccaac
     9121 acatcgccgc caaaaaattc gatgaattat tggcagcaaa aaccaaaggt gaaattaaat
     9181 taaaactgtt tccggacagc accttaggta atgctcaaac gatgatcagc ggcgtgcgtg
     9241 gcggtactat cgacattgaa atgtccggct cgccaaactt taccggctta gagccaaaac
     9301 taaatgtgat tgatattccg tttattttca aagatcgtga acacgtttac aaagtattag
     9361 acggcgaagt tggtcaaggc ttattaaaag atttagaagc ccaaggctta aaaggcttag
     9421 ccttctggga cgttggcttc cgtgctttct ccaactcaaa acaccctgtg accaagccgg
     9481 aagacatcaa aggcttaaaa gtccgcacca accaaaaccc gatgtacatt caagccttca
     9541 ccttactagg cggcaacccg gtgccaatgc cactttctga gctttacacc gcactcgaaa
     9601 cccgtgccgt tgatgcccaa gaacacccaa tcggcatttt ctggtcggca aaactgtttg
     9661 aagtacaaaa acatttaagt ttaaccaacc acggctacac cccgttaatc gtggtgatga
     9721 acaaagcgaa gtttgacggc ttagctcctg aattacaaac cgcattagtt gaagccgcaa
     9781 aagaagccgg tcaattccaa cgtgatctca acgtgaaaaa cgaaaaagag atcatcgaaa
     9841 aattacgcaa acaaggcatc gaagtgatcg aacaagtgga taacgccccg ttcaaagcag
     9901 cgattgaagc ggaagtccgc aaagctttcg tggaaaaaca cggcgatgaa ttattgaaaa
     9961 aagtggatgc gttagcacag taattgactt tattttagtc taaagctccc tccgtttggg
    10021 gggagttgtt gtatagactc tactatagga gagactatga aactgtttaa tttaaaaact
    10081 ttagccgctt tcgttgccgg ttttgcactg ttttcaaccg cacaagcaga aacctcattc
    10141 cgctttgcgt atgaagctcc tcgttctgat actcaacata ttgcagcgaa aaaatttaat
    10201 gatttgctgc aagaaaaatc ccaaaaaggg ctgaaattaa gcctcttccc tgatagcacg
    10261 ctcggcaacg cacaaacggc gattagcggt gtgcgtggcg gtactattga tattgcaatg
    10321 tccagctcct ctaactttac cgggttagaa ccaaaactga atgtgattga tattccgttt
    10381 attttcaaag acagagacca cgcctataaa gccttagatg gagagattgg tcaaggttta
    10441 ttaaccgcat tagaggctca cggtttgaaa gggttggcgt tttgggaagt tggtttccga
    10501 gcgttttcca actccaagca cccgatcacc aaaccggagg atattaaagg cttaaaaatc
    10561 cgtactaacc aaaacccgat gtatattcaa gccttctcta ttttaggtgc aaatccggtg
    10621 ccaatgccgc tttccaagct ttacactgca ctggaaacca aagcggttga tgcccaagaa
    10681 cacccaatcg gcattttctg gtcggcaaaa ctctatgaag tacaaaaata tttcagcttt
    10741 actaaccacg gttacacccc gttaattgtg gcgattaaca aagcgaaatt tgatgcactg
    10801 ccagccgagc aacaaaccgc attagttgaa gcggcaaaag aagccggaaa ataccaacgc
    10861 caacttaacc tagaaaatga gcagaaaatt atcgactcgc tcaaaaaagc aggtattgaa
    10921 tttgtggata acttagatac cacaccgttc aaagatgccg tatcggctga aacccgcaag
    10981 gcatttatcg agaaaaacgg tgatggctta gttaaagcca ttgatgcatt ggcgaagtaa
    11041 aaatagagct acaaagtgct cggttaaact aacctcacac gaatcgtgtg aggtacaagc
    11101 ggtcaaaatc cgcaaaattt ttacacatct ttcctcaaag gaggaacaaa tggcacagcc
    11161 cattttaagt atgcagcaca tcatcaaacg ctttggtagc catacggcgg tgaatgatgt
    11221 cagccttgat atccactcag gcgagatcgt tgccttactc ggtgaaaacg gtgcaggtaa
    11281 atcgacccta atcaaaatcc ttgccggtat ttatgaccgt gacgagggtg atattctttt
    11341 ccataaccaa aaaatccaat ctgcccacgc tattgagaaa ggcaacaaaa agcccatcgc
    11401 ttttatccac caagatttag gcctaataga atggatgacg attgccgaaa atatggcatt
    11461 tgtaatgggg ttccctcgca aatttggctt gattgactgg gcgaaagtgc gagagcagtc
    11521 gcaaaaagcc cttgattttg taggcattaa actctctgcc gacactcgag tgtttgattt
    11581 aagccgtacc gaaaaagcct tactcgccat tgcccgtgcg attgccgtca atgcagaaat
    11641 cttggtttta gacgaaccta ctgcttccct acctgcctct gatgtagagc atcttttcac
    11701 tgtgttaaac cgcttacgtt cccaaggtgt gggaatgatt tatgtgaccc accgtttaga
    11761 tgaagtgatc gccatttccg accgcatttt ggtgatgcgg gacggtttcc cggttgcaga
    11821 agggcaaacc aagctctatg acgtcaaagg cttggtcaaa gcgattgtag gcgaagagag
    11881 ccgtggcaga cagcgggtgg atttaccaac cgatactcct gaaattttaa gcctgcaaaa
    11941 tgtcgtggtt ggcgacaccg gcccggtgaa tttccgtctg cacaaaggtg agatgattgc
    12001 ccttgccggc ttgcgtggag cagggcagga agaaatcggc cgcttattat ttggggagcg
    12061 aaccttagac aaaggcgaaa ttcagttaga caagcggtca tattctgcta aagatccgca
    12121 agatgcgata gccaaaggtg tggcattggt tgcgggagac cgcacccgtg aaagcgtgtt
    12181 tatgtcaatg actaccagtg agaacctgtt tatcaacccg gtactaagcg gtcattcacc
    12241 attaaaacgc tactcccgcc gtgaagagtg gggtgagagc tggtacaaat tccaactatt
    12301 cgatattcgt ccgaaaaatt tgtttattga tgccagtgcg ttatccggtg gtaatcagca
    12361 gaaaatcgtg ttggcacgtt ggatgcacct caatacgcct gttctgattt tagaagaccc
    12421 gactgccggc gtggatgtag gagcaagggc agagatttac gacttgctca acaaagccct
    12481 tgcagaaggg gtcgcaatta ttgtgatttc caccgacttt gaagagatcg ctcatttgtg
    12541 taaccgagca ttagtcttca accgagggga aattgccggt gaactactga acgaagaggt
    12601 gactttcgcc aatttgctgg cgttggcatc ggatagtcag tcgaaaaaac aataacttac
    12661 tctcgcccgc ttgcgggaga gagacagcta aaaatagcgt caggctattt ttagcagaga
    12721 gagggtaaac aagcggtcat ttttcttaaa aaatattgcg aaccctctcc ctccgaaagc
    12781 ctaacggctt tctcctccct ctcccgcaag cgggcgaggg gaaaatgcag aattttaacg
    12841 gagtaattta tggctcaaac aaacatcaaa tcaaccgcac tggaacaacc ggtatctatc
    12901 gcccgtgacg gttttatggc atggttttca caaatgtcca ctcgttatgg cttactgtgg
    12961 ttatgtgtgt tattggtgat tattttctca cttaccaccg aatctttcgc ctcaatgctg
    13021 acattaaatg cgattttaga aagtaaatcg aaaattgccc tacttgcttt ggcagccaca
    13081 actacaatga ttgtcggcaa aatcgacctg aacgtgggtt tcggtattgt gttgtggcac
    13141 attcttgtga ttactctgca agtccaatac ggcttctcgt ggtatatggc gatcttaatc
    13201 gtgttagtga ttgccgctct ttacggcttg ctgaatggga ttttagttgc tcttgctgat
    13261 atcgatagct ttgtggcaac acttggctca ggcacagtgc tttatgccgt agcactctgg
    13321 cactcgggcg gtcgccaaat tgtcggggat ttacccgatg aatttatcgc actcaacagc
    13381 acagaaattt tcggtatccc gatttctgct ttctatgtgc tggcagtggc tatcgtaatg
    13441 tggttggtga cagaacatac cccgaccggt cgttgtatgt atgcggttgg tggcaaccca
    13501 acggcagctc gcctaaatgg tatcaatatt aaaaaataca ccattgtgcc gtttattgtc
    13561 tctagtgtaa tcactgcttt cacaggcgta ttgatctctg ctcaacaagg cgttggtcag
    13621 gcaagtgtcg gtatggacta cttactgccc gcattggttg gggcattctt agggagtacc
    13681 actattcgcc cagggcgtat taacgtatgg ggaacagtgg tggggattgc cattcttgcc
    13741 atcggtattt caggtattca acaattcggt ggagcgttct gggttgagcc actctttaac
    13801 ggtgcaacac tgttactttc catcactatt gcaggctatg cccaacgcaa acgcttgtta
    13861 aatcaaaaag ctgtacagaa aaaatcgacc aactaagagg atttgattat gaaaaaagca
    13921 tttcgtacaa ccttaactgc aatcgcaacc ttaactgcta ttgctttcgg aaataatgcg
    13981 tttgccaatg acgacccgtt cgtacaagaa gcaaaacagc ttgtcgcagc agctacggct
    14041 aagcaagaaa aatgggacgg accgacaacc ggccctcaat tacaaaaaga gaaaactgtg
    14101 atttttattg cctctgatat gaaaaacggt ggtgtacttg gtgtgattga cggaatgaaa
    14161 gaagctgttg gcgtaacggg ctggaagtta gatgtattag acggtgcagg tacggtaaac
    14221 aaccaactcg ccgcacttaa ccaagcgatt gcccgcaaac cggatgcgat tgtgattggt
    14281 ggctggaacc cgaatgtggc gaaaatccca ctacaaaaag cgactaaaaa cggcattaaa
    14341 cttgcagctt ggcacgcaac accggagccg ggtgcgattg ataaatacgg cattttctac
    14401 aacgtgacct ctgactcaga cgaaattgca cgcttatcgg caatgcttgc ggtggcagaa
    14461 tctaacggta aggcaaaagc gattatttta accgactcgc tatatgaaat tgcgttacgc
    14521 aaagcgaacg tcatgaaaga agttatcgaa aaatgtaaag agtgtaaggt gttagagttt
    14581 atcgacacgc cattggcgga tacctcaagc cgtatgccta acctcacttt cagcttactg
    14641 caaaaatatg gcgacgattt agagtatgcg ttggcaatta acgatctcta ctttgacttt
    14701 atggctccgt ctttacgcac cgctggtaaa gataaaccgt ttagcatttc cgcaggtgat
    14761 ggctcagtaa cggcttatca acgtattcgc aatggtgata aacagtttgc aaccgtccct
    14821 gagccactta acttacacgg ctggcagtta gtcgatgagc taaaccgtgc tttcgctaac
    14881 gaaaaaccgt ctggttatat cacaccggct cacctagtga ctaaagaaaa tgtggcgttt
    14941 gacggtggtg aaaacaacct gtatgcccca caaaacggtt acaaaggcgc ttacaaagcg
    15001 atttggggcg tgaagtaatg acgtaggggc aaataaatat ttgcctcgaa caagcggttg
    15061 ttttttgcaa agattttgcg aaatttaacc gcttgtcata aaaaatagag agggtaatac
    15121 aaatgagcaa taaatttatc cccaaaatct ccaaccctcg ttttaatgag ctggttggga
    15181 atacgtttga acttgaagag ctttttgata aggcaatctg ggcagaaggg ccggcgtgga
    15241 gcaagccaca acaagccctc tattttagtg atgtaaaagg ttgcacaatg taccgctgga
    15301 cagagcaaga tggtacacag gttttccgcc agccgtctta tttttctaac ggcaatgcgt
    15361 ttgatgcaaa cgacaacttg attacctgtg aacacggcaa tcgaggtatc agtttaacca
    15421 cgccaagcgg tgaatatcgt tttttggtcg gtcaattaga cggaaaacga ctgaactcac
    15481 ccaatgatgt ggtggtaaaa tcagacggca tcatttggtt cactgatcca ccttacggta
    15541 ttttgtcgga taacgaaggc aaacagtcgc caagcgaaat tatcggttgt tatatttact
    15601 gctatgatcc gaaaaccgac aagctgaata ttgcaacttt caacacgatg cgaccaaacg
    15661 gcttggcctt ctcgtcagac gaaaaacaac tctttgtggc agatatgtcg agcgtggaat
    15721 ttgacgaaga tggcttacac cacttagtcg tatttgatgt ggacggtaat aaactaaaaa
    15781 atcgccgaga tgtggcggaa attagtcccg gcattccgga cggcttccga attaaacaaa
    15841 acggcgtaat tttctgctct tgtgaagagg gaattattgc tattttacct gacggcacgg
    15901 agctgggacg atttgtgctg ggtaagctaa cctcaaattg tagctttggc gatgatgaac
    15961 aaacactgtt tattacttgt tcgaatagcg tttatcgttt aacaattaaa tagcagaatg
    16021 cattaagtgc gtctgttgta gcattacaag cggtttcttt ttcaaaattt tttacaaaca
    16081 aataatggat aacaaaatga actacacact cggcattgat tgcggtggga cttttatcaa
    16141 agccgctctt tttgatacac aaggtaatat tcaagccctt catcgtgaaa acgtttcggt
    16201 aatcagtgaa caggcaggtt atgcagagcg tgatatgtca caactctggg ctatttgtgc
    16261 ggaagtagtg cgttcaacca ttgaaaaaag tggggtaaac ccagcggata ttaaagcagt
    16321 agggatttcc gctcaaggca aaggggcgtt tttactggat aagcagaatc gaccgttggg
    16381 gcgtgcgatt ttatcttccg accaacgttc ccttgagatt gttaaacaat ggcaggctga
    16441 aggcattccg caacaacttt acccgattac ccgccaaact ctttggacag gacaccctgt
    16501 gtcgattttg aagtgggtgc agcaaaatga accggagcgt tatgcacaaa tcggggcggt
    16561 gttgatgtcg cacgactatt tgcgtttttg cttaaccggt gagttgcatt gcgaagagac
    16621 caacatttcc gaaagcaatc tttacaatat ggaaaaaggc gaatacgacc aacagttagc
    16681 cgatttgttg ggaatgccgg atgtgttgga gaaattacca ccagtgattg aaccgaataa
    16741 aatttcggga tatgtgactg aaaatgccgc caaattaacc ggtttagcgg tgggtacgcc
    16801 gatagtcggt ggcttgtttg atgtggtttc taccgcacgt tgtgccaatt tagatgacga
    16861 aaccaaactg aatgcagtgc tgggaacgtg gtcggtggtg agcggcatta ccgatcatat
    16921 tgatgcaagc caatcattac cttttgttta tgggcgttat gccgaggcag ggaaatttat
    16981 cgtccacgaa gcaagcccga cttctgccgg taatttagag tggtttgtga atcagtggaa
    17041 actcgattat gtgaaaatca accaagctgt tgcccaatta ccgccggcgg caagctcggt
    17101 gctgtttgtg ccgtttctgt atggctcaaa tgcaggctta ggaatgcaag ccggtttcta
    17161 tggtatgcaa gcccaccata cccaagccca tctcttgcag gcaatttacg aaggagtgtt
    17221 gtttagcttg atgagccatt taaaccgtat gtttatccgc tttccaaagg ctgacgtgct
    17281 gcgagttacc ggcggaccgg tgaaatctaa agtttggatg caaatgttgg ccgatttaac
    17341 cggaatgcgg cttgaaatac cacaaatgga agaaaccggc tgcttgggtg cggcattaat
    17401 ggcaatgcaa ggggttgggc tggatataag ccaagttcaa gccctaaata atgaaatgca
    17461 ggtgtttgag ccaaatccgg aaaattatgc ggcttatcag acgaaatatc agcgttacca
    17521 aaaattaact gaagcattaa atacaatgat ttaacctgaa ttta
//
LOCUS       DANLUA010000030        17256 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLUA010000030 DANLUA010000000
VERSION     DANLUA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17256
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(259..1392)
                     /locus_tag="PXB44_000757"
     CDS             complement(259..1392)
                     /locus_tag="PXB44_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL5281264.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1409..2221)
                     /gene="dapB"
                     /locus_tag="PXB44_000758"
     CDS             complement(1409..2221)
                     /gene="dapB"
                     /locus_tag="PXB44_000758"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL5281265.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(2272..2649)
                     /locus_tag="PXB44_000759"
     CDS             complement(2272..2649)
                     /locus_tag="PXB44_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281266.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(2639..3112)
                     /locus_tag="PXB44_000760"
     CDS             complement(2639..3112)
                     /locus_tag="PXB44_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281267.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(3180..4268)
                     /gene="rlmM"
                     /locus_tag="PXB44_000761"
     CDS             complement(3180..4268)
                     /gene="rlmM"
                     /locus_tag="PXB44_000761"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL5281268.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(4268..4660)
                     /locus_tag="PXB44_000762"
     CDS             complement(4268..4660)
                     /locus_tag="PXB44_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL5281269.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(4767..6168)
                     /locus_tag="PXB44_000763"
                     /pseudo
     CDS             complement(4767..6168)
                     /locus_tag="PXB44_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(6323..8680)
                     /locus_tag="PXB44_000764"
     CDS             complement(6323..8680)
                     /locus_tag="PXB44_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL5281270.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(8816..11422)
                     /gene="acnB"
                     /locus_tag="PXB44_000765"
     CDS             complement(8816..11422)
                     /gene="acnB"
                     /locus_tag="PXB44_000765"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL5281271.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(11442..12689)
                     /gene="icd"
                     /locus_tag="PXB44_000766"
     CDS             complement(11442..12689)
                     /gene="icd"
                     /locus_tag="PXB44_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL5281272.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
     gene            12923..14203
                     /locus_tag="PXB44_000767"
     CDS             12923..14203
                     /locus_tag="PXB44_000767"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL5281273.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
     gene            complement(14276..15505)
                     /locus_tag="PXB44_000768"
     CDS             complement(14276..15505)
                     /locus_tag="PXB44_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281274.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVK
                     SDENGQDVRTQLLNKLA"
     gene            complement(15472..16461)
                     /locus_tag="PXB44_000769"
     CDS             complement(15472..16461)
                     /locus_tag="PXB44_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5281275.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDGAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            complement(16477..16995)
                     /locus_tag="PXB44_000770"
     CDS             complement(16477..16995)
                     /locus_tag="PXB44_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5281276.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            complement(17005..17076)
                     /locus_tag="PXB44_000771"
                     /pseudo
     CDS             complement(17005..17076)
                     /locus_tag="PXB44_000771"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17083..>17256)
                     /locus_tag="PXB44_000772"
     CDS             complement(17083..>17256)
                     /locus_tag="PXB44_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5281277.1"
                     /translation="PRRVFERSAEKKKIHNLISRAHYDMATNEDSASEHLELFGEDIA
                     EDSFDYPKPEKLI"
BASE COUNT         4968 a         3903 c         3231 g         5154 t
ORIGIN      
        1 ctaaaaaatt tgaacaaacg aagcgacagc gttagcaaaa acttaaaatg atgtacggat
       61 ttaaaaatct gttcgtttgc ttgttttata agcaaactaa tttcgcataa ttattattat
      121 gttaaataga tagttaaaat aacacattta attaaatctt ttcgtgtttc tctcctcctt
      181 tattaaaaaa gaaaacggtc agattggtaa aatctatacc taatatgacc gcttgtgaat
      241 acaaaaaaat tacttcaatt acttcaactg tttaaaggct tgcagtaaat cagctttaat
      301 atcttcaacg tgttctaaac caacggaaaa acgcagtaaa ttattcgtaa tgcctcgtgt
      361 aatacgttct gcttccggaa tatccatatg ggtttgtgtt gcaggataag taataaaact
      421 ttctgcaccg cctaaacttt ccgcaaaggt aatcaattta attgccttga gaaatttcgc
      481 tacccactct tctttttgta aacggaaaga taacatacca cttttgcctg aatataacac
      541 gctgtcaatt tcaggttgct ctgctaagaa tttagccagt tcggtggcat tagactgatg
      601 gcgttccatt cgtaatgcca aggttttcat accacgcacg gcaaggtaag aatcaaatgg
      661 cgacagcact gcaccggcac agttttggaa atagaataaa cgctcggtca aattctgccc
      721 gtttactttg gtagcatcag aatctttcgc aacaattaag ccggctaaca catcattatg
      781 ccccgaaaga tacttggtcg cactgtgaat cacaatatca gctccgtgct caatcggacg
      841 gaataacgct ggggttaaga acgtattatc aacgatcaac attaaattgt gttttttcgc
      901 aattttagca atggcagcaa cgtcgcactc ctccatcaat ggatttgacg gcgtttccac
      961 gaaaatcgcc ttagtattcg gtgtaatagc tgcttcaatc gctgctaaat ccgctgtatt
     1021 aacataaacc ggctttaatg tgccgtgatt tttgtggcta aaatcaaaca agcggtaaga
     1081 accaccataa acatcgctgg aaataatcca ttcatcaggt gctttaaata atgacattac
     1141 tagctgaatc gctgccattc cagatgccat cgcaaaaccg gcatcaccgc cttctaaatc
     1201 cgcaatcccc tgctctaata cagctcgagt tgggtttttg gtacgggtgt aatcccagcc
     1261 ggtagattcg cctaagccat cacgcccgta agcagttgat aagataatcg gcattgccac
     1321 cgcccctgta cgaggatcag ttctgttacc gatttgcact aatgtggttt caatattatt
     1381 gaattttgtc attgttatta tccttcaatt acagattatt tagatccaat acgtctgtca
     1441 tatcaaacaa gccattttgt ttgccattta accattttgc agcacgcact gcgccattgg
     1501 caaacgtcat tcgactagag gctttatgtg cgatttcgac acgttcgcct tcatcagcga
     1561 accatacact atgctcccct acaacatcac cggcacgaat agtagcaaag ccgatttcat
     1621 cacgtttgcg ttcgcctgta ataccgttac gttcaaatac gccatgagtt ttcaagtcac
     1681 gcccaagagt tttggcaata tgttctccca ttgataaagc cgtacctgat ggtgcatcaa
     1741 ctttatggcg atggtgtgcc tcaatcactt caatatcaca ataatcgccc attacttttg
     1801 ctgctttttc gagcagttta aacaccagat tcaccccaac gctgtagttt gaggcaaaaa
     1861 cgataccgat tttttcactc gctgctttga ttgcagcttt acctgcatca tcaaaaccgg
     1921 ttgtgccaat caccatttgt ttatgatgag ccacacaaat atccaaatgt tctagcgtgc
     1981 cttcagggcg agtaaaatca attagtaaat caaaacctgc tacattttgg gcgacatcat
     2041 cagaaatcac cacacctaat gtaccaaccc cggctacttc accagcatca ctacctacta
     2101 atgatgaacc tttacgctca aaagcagacc ctaattctac gccttgtgca ttatggacgg
     2161 cttgaattaa ttgacgcccc attcgcccac cggcaccgac gattccaatt tttaatgtca
     2221 tatttgttct tcctttttat atttgttttt tacaagcggt tacattttta attaattttg
     2281 caaatagctt tctgatggca atttaaaagg tgggtgagaa gtaaaatcaa caatttgaat
     2341 aaagttttca cctttcacat aatgtaaaat atactcagaa gtataattac caaatccttg
     2401 actggtatca tattctggaa tgccgatatg ataatgccat aaagaatatt tttgtgcttt
     2461 agccaccttc tctgcccaat aaggatcatt cgttggcaca tcatcagaac ttttatttcg
     2521 accagctaga ttttccaatc cataaatttc aacgtgttgt ttaaactgca ggattttgac
     2581 tagatctttt tcaggcaaat attgcaattc ggctgaaatt tttgtaccaa ataaaacttt
     2641 aatgcccatt tgcaacacct ttcaaccagt ttctaaattc attaacattc gttatatctt
     2701 taggcacttc gtgaaaagta tcatttaacc gctctttcat tcgctctaag tcaaaattaa
     2761 atgcggttgc ttgagagagt tgctgttcaa ttttgcgaat attggacttc atattatcca
     2821 atatagccat tgtccttgag ccatcagtaa aatactgttg ggtatcgaaa atcaccacct
     2881 tattttgatt taggtatttt ttgaaatcgt ttaaatcaat ttcagcccaa aagtttggat
     2941 ttttttcgat agtttcaata caggcaatta ataattgatt tgccattgta atacgaaaat
     3001 ttaacaatga gatttgctgc tctgttgcag gtttatcatc agctgcgaaa tcaaatgccg
     3061 gataaatcac tgcaagcgtg tcagccatag ataagggtaa atttttcatc atctctcctt
     3121 acggaagaag ttgtcattat ctactggatt acttaaaaaa acaacttatt cattttatgc
     3181 tatttttgcc ccatcaccgc aatatgtacc gtaatttcct cacggtcgtg gtagaggtgt
     3241 ttagcttgca ttttgaacca aaacccgtgt ttcgttagct cttcttccaa gaaattgagg
     3301 catagttgca cttcttgata acgttttttc atcggcagtt ttaggttgaa aatcgtttct
     3361 cgacaccaac cgttagtcag ccattttgca atgagtttcg agatacgaat cggctgctct
     3421 accatatcac aaaccagcca atcgatttgt ttacgcttag gcggttggaa tttaaaccca
     3481 tcttcagggc aatgctcaat tcttccggtt tcgtgtaaac ttgctgccat tttgccgtga
     3541 tcgactgcat agacaaaaac accacgtttg actaattgat aagtccaacc acccggacaa
     3601 gccccaagat cgacccctac catcgtatca ttaaagcgtt ttttctcctc tgcttgcgga
     3661 ataaaagtta aaatcgcctc ttctaattta agcgttgagc gactaggtgc ctcagccggg
     3721 aattttaaac ggggaatccc cataaaaaac ggcgatttat tatggttgta cgcatagccc
     3781 acatagcaag ctcccggacg agtaaataaa atatgcaagc tcacactacc ctgcatattt
     3841 tccgatttcc ccaaccaacc ttgttttttc aatgccgacc gtaatggcac agtaaattta
     3901 cgacagaaag ttaataattc tttcgcttcg ttggtatccg gtgtttcgac aaatacatca
     3961 gaactttggc gaggattaag ttcggcatat tgggctaaaa tcggcgaaat tcgatcattt
     4021 tcagacagat tttgcaacaa atccccaaca accaacattt ggcgaacaaa aataagctgt
     4081 tcaaacttta acgcttgggc taatcgatct gcttcccctg cttgatagca ctcaaaaatc
     4141 acatagccac tattttcttt aaggttggca aagccaaaaa tgccctgttg tgcagcttta
     4201 tccgtgattt cgcctgctac ctctttttca aaccctgctc ggcaatataa ggctaattta
     4261 ttgaacatta ggcttttctc acactgatat aaattaacgc aatccaacca agcataaagc
     4321 agagaccacc aataggcgta agccaatgaa tagtcggcac atttaacgcc agtaagtaca
     4381 ggttgccact aaaaagcaga atgcctaacg cccaattgct tccgatgatg ttaaaggctt
     4441 tggctctaca aacgggtgga ttttgcccaa aatttgcaat ttggaataaa cctaaagcta
     4501 aaatggcaag ggtgtgaaac atttgatatt taatccccgt ctcaatccag cctaaggctt
     4561 taggcgataa ttgagccgat aatgcgtggc tggctatcgc cccaaatgcc acagtaaata
     4621 agccgctgaa tgcggcaatt gctacaaatt tgtttttcat aagttgaaga taggtatgat
     4681 gttagatggc ttactatatc atattatcga aaccaacaag cggtgaaatt ttgcaaaaaa
     4741 attacaaaaa atcaccgttt gtatgcttaa tggttcaaga taatatccac atcttccgat
     4801 ttatcgtgaa ccccaaatct atctactaag gttttgctgg cctcatttaa tccgataatt
     4861 tctacctcaa catcactttt tcgatatttc aacacaactt tatctaacgc tgaaactgcc
     4921 gttacgtccc aaaagtgagc gtgggttaaa tcaatgtgga cttttttcag ttctgaacgg
     4981 gtaaaatcaa attgtgcgat aaaatcatca gatgaggcaa aaaatacttg cccgataaca
     5041 tcataacgca attcctcttt ctctatagca cttgttttaa ccgccatata acgtccaatt
     5101 ttattcgcaa agaataatgc cgagagcaac acgccggaaa atacaccaag ggctaagtta
     5161 tgtgtataaa ccacaattgc aacagtgact aacatcacta cattaaagcc gattgggtgc
     5221 gtttttagct cttttaagga atgccaatta aaggtgccga tagagaccat aatcatcact
     5281 gcaactaatg cagccatcgg aatcactctc aaaatatcgc tcaaaaagac cacaaaaatc
     5341 agtaataata ttggcaatgc cctgcccttt acattcacgg tttttatcgc tgtctgtctc
     5401 tgtcatttca tcaacaatcg ttgccgtcat taaagattcc aataacccta ctgcggccaa
     5461 gctgaaagaa taaggcaaaa taatccataa ggtgtctaaa ttaagcggaa tatcgggtaa
     5521 taaaaacacc ggtaaggtat cgggtaattg ccccatatcc ccaacggtac gaacatcaat
     5581 gccttgccat aatgtgaaaa gtgttaaagc aataatggtc actaatggtg agggaatgat
     5641 ttttccaatc accggtacat aaggaaaaag ataaatgata gcaagccctg ctaccaccag
     5701 tgcataagta tgccaagttg tattttgtaa ctcaggtagt tgtgccataa aaattaaaat
     5761 ggcaagtgca ttaacaaacc caatcactac ggctcgtgcc acaaaccgca taagcgagcc
     5821 gagttttaac caaccggcaa taatttgcaa cacgcctgtt aataaggtgg ccgctagcat
     5881 atattgtaaa ccgtgatccc tcactaagcc tgctaatacc agagccattg caccggttgc
     5941 tgcagaaatc atcgccggtc tgccgcccac aaaactgata attactgcaa tacaaaatga
     6001 ggcatataaa ccaatttgtg gatcaacccc tgcaataatt gaaaatgcta tggcttctgg
     6061 aattaacgcc aaagccacca ctaaaccaga gagaacatca cctcgtacat ttgaaaacca
     6121 ttgttgttta attaccgtag gcgaaaattg ctgaaataaa gttgtcatta ttacccttaa
     6181 ataaaaagaa aagccgagca aagtcggcta aaaatgaggc gagagagtag caaattagtg
     6241 aaaagtcgtc aaataaacaa gcggtcttct ttcgctcttt ttttgcaaaa aatagtagaa
     6301 aaatgaccgc ttggattaat tgctattcac taaaaaaagg ggctttaaac accgtcactt
     6361 caggtgcatc tcccacatat ttttccactt gaaccgtggt agtgttcggt gcattgccct
     6421 gcccgagttt tgatgaaccc tcattacggg ttagcacatt cgggttgccg tttttgcata
     6481 gtggagtatc tgttttgcct aaatcttgcg ggtcatacca accaccttcg tataacgcta
     6541 ctgtgccttg aatgatgcca tcagtcacca ctgctcctgc caacacctgc cctcgcttgt
     6601 taaaaattcg cacaatatca ccgcttgtaa tgcctctgtc agcggcatct tttgtgtgaa
     6661 ttagcattgg ttctctgccg tttacctcgt aatattgacg taatgaggag taagcaagct
     6721 ggctgtgtaa acggtattta atatgcggtg taactaatgc caacggctcg gcttcgttgg
     6781 ttttgcctga atattcgtga tgttcaaacc aagtcgggtg tcctttacaa tcttcataat
     6841 tcatcttggc aatggtttcg gagaaaatct caattttgcc tgatggcgtt gccaatttgt
     6901 gtttaatcgg atcagtacga taatcttgat agcgaacgaa agtattattt tgatgttctg
     6961 cctcaaagct caacacttta ttttccgccc aaaatgcctc gaaatccagc aatggcgtat
     7021 tagctttttc ccccgcctca aaggcaactt ggtaaaattc cttaagccat tccatttcgc
     7081 ttttgccttc ggtaaatgct tgttctttac cggctcgttt cgctaattcg gcaaaaatat
     7141 cgtaatcatt cttcgcctca aattgcggtt caaccacctg tttcatcggg ataatatgtt
     7201 ttgctacata atcaccggca acggtgagat cgtttcgctc gtagctggtg gttgcgggca
     7261 atacaatatc tgccattcga gcggtcggtg tccagtaaca ttcattgacg atcatcgttt
     7321 ccggctgttg ccacgctttc actaaatagt tggtatccgg gtggtgagca aacggattac
     7381 cccccgccca atagactaat ttgatgttcg ggtaatgtaa tgtttgcccg ttgtaatcat
     7441 aaggctgatt cggtttgagc aacgcatcgg caattcgaga caccgggaaa aacggcatat
     7501 cggggttagg tttacctgca ttgattgccc ctaaacgaac accggaatta acatttagcc
     7561 cattggtaga ataacgataa tttaagccga aaccgccgcc cggcaagcca atttgcccga
     7621 gcatagcggc aagagtcact aacgcccagt ggctttgctc cccgtgttgc aggcgttgca
     7681 agccccagcc ggacatcaac attgtgcggt gagtaacaaa atcatttgct aaggttttaa
     7741 tcacctcctc cggaatgtca caaatttggc tcgcccactc tgctgtttta ggtaaattat
     7801 ctgcttttcc taacacataa tcagcaaatt gctcgaaacc aacagtatat ttagttaaga
     7861 agtcgtgatc gtgcttgttt tcagtaataa gggtgtgggc gatccctaaa atcagtgcaa
     7921 catccgtgcc ggtattaata ggcaaccact ctgcctctaa aaattgacag gtctcgcttt
     7981 tcaccgggtc gatacaaata atccgtttac cgcttgcttt taatttggcg agataatcaa
     8041 tcccctccgg ttcggcagag gtccacgcaa tgcgaagtgt attcagtgga ttagacgacc
     8101 aaattaccaa taattctgtg ctttctacaa ttgtgtgcca actggtttgt tgagcataca
     8161 cctcaatatt gcctaataca taaggcataa tcacttgcac cgcaccggtg gaataatcgc
     8221 ctttagtatt ggtaaagccg cctgtgacgt ttaaataacg ttgcaaaagc gtgcgagagg
     8281 cgtgcagtga accactgcta taccagccgt aagaaccgcc gaaaatggat tcgctgccat
     8341 attgctctct tacccgttgg atttcgccgg aaaccagctc aaaggctttg tcccaagaga
     8401 ttctcaccca cttatctcgc cctcgcaaag tggtgtcttt attcccttcc aagtagcctt
     8461 tccgcaccat cggatatttc acccgaaccg ggctatagag ctgatccgcc accacatctt
     8521 gcaattcgtt ttggtgattt ttcgctaaac tgccctctga tttcaccact tttccgtttt
     8581 ccaccgttac atttaatgca ccccagtggg cggcggtggt aatggtattt ttcggtttat
     8641 ccaaaaagac aaacttccct ttttctaatg ctgtgttcat ttaaattaat gccttttctg
     8701 ttacaaaatt gagaggaatt tactggatta agccaaaagt tgcaaccaca agcggttgtt
     8761 tttagccaaa aatttgcaaa acaaaaccca cctaaaaagg tgggcttcaa ggaagttaaa
     8821 cggcaacttg aacgattaca ttgtccgctt ttttcacata actattgatt tggtcaaagt
     8881 tcatatagcg gtaaatatcg tccttatctt tctgcaagtt attcatatag tctaggtact
     8941 cttgcggtgt cggcaattta cccaataacg cagaaactgc ggctaattct gctgaggcta
     9001 aatacacatt tgccccttgc cctaaacggt tcgggaagtt tcgggtagag gtagaaacca
     9061 cattagcatt atttgccact cgggcttggt tacccataca gagagaacag cccggcatct
     9121 ctacccttgc tccgcttttg ccgtaaatgc tgtaatagcc ctcttcggtc agcaagcctg
     9181 cgtccatttt agtcggcgga gcaatccata atttagtcgg caattcgccc tgtgcttggc
     9241 ttaacagttt acctgccgca cggaaatgcc cgatattagt catacaagaa ccaataaaga
     9301 cttcatcaat tttatcgccc tgtacttgag aaagcaggcg agcatcgtca gggtcattcg
     9361 gtacgcacac aatcggctct ttgatttcat ctaagttgat ctcaatcact gcggcatact
     9421 ccgcattttc gtctgcctct aaaagctccg gattatccaa ccacgcttgc atattggcga
     9481 tacggcgttc tagcgtgcgt ttatcgtcat agccttccgc aatcatccat tttaaaagcg
     9541 taatattaga attcagatac tcgataatcg gctctttgtt gagcttgatg gtacaggctg
     9601 cagcagagcg ttcagcagaa gcatcagaaa gctcgaaggc ttgttcgatt ttcagatctt
     9661 ccaacccttc aatttctaaa attcgaccgg agaaaatatt tttcttacct tttttctcca
     9721 ccgtcagcaa gccttgctga atggcataat acggaatagc gtgaaccaga tcccgtaagg
     9781 taatgcccgg ttgcattgtg ccggaaaaac gcaccaagac cgattccggc atatctaacg
     9841 gcatcacccc tgttgccgca gcaaaggcaa ctaagcccga accggcaggg aatgaaatcc
     9901 cgattgggaa acgggtgtgg gaatcgccgc ctgtaccgac agtatccggc aataacatac
     9961 ggtttagcca agagtgaatc acgccatcac ccgggcgaag tgacacaccg ccacggttca
    10021 taataaaatc aggcaaagtg tggtgggtaa ttacgtccac cggtttcggg taagcggcag
    10081 tatggcagaa cgattgcatc acaagatctg ccgagaagcc taaacaggct aaatctttca
    10141 gctcatctct tgtcattggt cctgtggtat cttgtgaacc gacagacgtc attcgaggct
    10201 cgcaatattg ccccggtcga acgccttcta cgccacaagc tcgccctacc attttttgag
    10261 caagggtgta gcctttgtta ctctctgaaa ccgactgcgg tttaataaat acatcacttt
    10321 caggcaagcc aagtgctact ctggctttat gggtcaaacc acgtccgata atcagcggaa
    10381 ttcgcccgcc tgcacgcact tcatcaagca atacttcggt ttttagctca aattcggcta
    10441 agacttcatc ggagttgtgt ttgcaaattt tgcctgcata agggtaaatg tcgattacat
    10501 cgcccatatt caggttggtt acatccactt cgatcggtaa agaaccggca tcttctaatg
    10561 tgttaaagaa aatcggggca attttaccgc ctaacaccac cccaccggca cgtttgtttg
    10621 gaatgtaagg aatatcttcc cccataaacc aaagcacgga gttggttgcg gatttacgag
    10681 aagagccggt gccgactaca tcaccgacat aggctaacgg gaaacctttt tcttttaatg
    10741 cttccagttg cttaatcggg ccggtatgac cgttttcatc ggggaaaata ccgtcacgtt
    10801 catttttcag cattgctaat gcgtgaagtg gaatatcagg gcgagaccaa gcgtcttgag
    10861 ccggggaaag atcatcggta ttggtttcgc ctgtgacttt aaataccgtt accgttaatt
    10921 tttcggcaag tttcgggcga gaggtaaacc aatcggcatt cgcccaagat tccaatactt
    10981 gttgtgcaaa cggattaccc tgatctgccc gttcttttac atcgtggaaa ttatcaaaca
    11041 tcaataatgt tttagataag gcttctaccg caatcggtgc aagctcaggg ttatccaacg
    11101 cagacaataa ggtttcaatg ttataaccac cttgcattgt gccgagtaat ttcactgcgt
    11161 gagcaggcga aatgagcggc gatgtggcaa cccctttggt aatatcggct aaaaaggcgg
    11221 ctttaacata agcggcttca tcaacacctg ccggaatacg attggtaaaa agatccaata
    11281 aaaagaccgc ttgttcagcc ggtgggtttt tcaataattc aaccagttga gccgtttgtt
    11341 ccgcagaaag tggctgaggc acaacaccca gtgccgcacg ttcatcaacc tgttgttgat
    11401 aattttctat aaaattttcc atcatcattc cctctccaac tttacatatt ggcaataatg
    11461 tcttgtgcaa attctgaggt tgagcgtaga gttaccccct ccagcatttc cgcaaaatca
    11521 aaggtgacag ttttattggc aatggtttta gacaccgctt ttaccactaa atcagccgct
    11581 tcaacccaac ctaaatggcg taacatcatt tccccactta aaattaatga cccaggattg
    11641 cctttatttt gccctgcaat tttaggggct gtgccgtgtg tggcttcaaa aatagccgct
    11701 tcaaacccaa tattcgctcc cggtgaaata ccgattccgc ctacttgtgc ggctaaagca
    11761 tcagaaatat agtcgccgtt taggttcagt gtagcaatca catcatattc ggttgggtgt
    11821 aacaagattt cttgtaaaaa ggcatcggca atgctgtctt ttataatgat ttcctcaccg
    11881 gttttcggat tagtcaagct catccaaggg cctttatcaa ttaattttgc cccaaattct
    11941 tccgcaactt ggtagcccca ctctttaaat gctccttcgg taaatttcat aatgttacct
    12001 ttatgcacaa gcgtgagcga tttacggttg ttatcaatta catattgcaa tgcagcacgc
    12061 accaagcgtt gtgtgccttg cttagaaacc ggcttaattc cgataccgca atcttcggta
    12121 aagcggattt ttttcacgcc catctcttgt tgtaaaaatt cgatcacttt attggcttca
    12181 ggcgaaccgg caacccattc tacaccggca taaatatctt ccgagttttc acggaaaatc
    12241 accatatcca ccagctcagg gtgtttaacc gggcttggcg taccgtcata ataacgaatt
    12301 gggcgtaagc agttgtataa atctaatcct tgacgcatcg ccacatttaa agaacgaata
    12361 ccgccaccca ccggtgtcat taaaggacct ttaatcgcca cgtgatattg gcggataagc
    12421 tctaaggttt catccggcaa ccaagtgttc tcaccatata atttattcgc cttttcgcct
    12481 gcgtaaattt ccatccaagc aatttttcgc ttgccttgat aggctttttc taccgccgca
    12541 tctagcactg ctttcattgc aggagtaaca tcaacaccga tgccgtcacc ttcaataaat
    12601 ggaataatcg gattatccgg aacttgtaaa gaaccgtctt ggttcagttg aatagtttcg
    12661 ccttgtggaa tttggacttg tgtttgcatt gattttaccc ccgtaatgac gaaaaagtag
    12721 tgacgaaaaa tgaatgttga caatcagcca acaatggatt aactataaac agtaaatttc
    12781 taaatgtaaa taaagcaaaa tgggctgaga gagaatttgt gatctaacgc acatttatgt
    12841 aaataaaatg taaaaaatcg ttgtgattct tttggaaatc atctagttta tcttataaat
    12901 ctcaaataaa taggagtaag ctatgtcaaa agcaacatta acactcgaaa caggtgaaaa
    12961 acttgaactc aatatgcaaa aaggaacctt agggtatcaa acgattgata ttcagccttt
    13021 cttaaagcat aagttatttg cttatgatcc cggattggtt tcgaccgctg tatgccgatc
    13081 caatatcact tatgttgatg gcgatgaagg ggttttactc tatcgaggct acccgattga
    13141 ccaattagcc aaacattctg attacttaga agtcggttat atgctgttat tcggcgaacg
    13201 ccctaccaaa gaagaataca aagacttcgt tcaactcatc aaaaaacaca ctcttgtaca
    13261 cgaacaacta accaaattct tctccggttt ccgccgagat tcgcacccaa tggcggtgat
    13321 gtgtggggta agtggagctt tggctgcctt ctaccacgac gccattgatg taaacaacca
    13381 agcccaccgt gaattaaccg ccattcgttt gttagccaaa atcccaaccc ttgcggcgat
    13441 gtgttataaa tactctatcg gtcagccgtt tatgttccca caaaaccact tatcttacgc
    13501 cggcaacttc ttatatatga tgtttgccac tccttgtgaa ccgtacgaag tgaaccctgt
    13561 gttagagcgt gcgttagacc gaattttcat tttacatgca gaccacgaac aaaacgcttc
    13621 gacctcaacc gtgcgaaccg ccgcttcatc aggtgcaaac ccatttgctt gcattgctgc
    13681 cggtattgcc tctctctggg gacctgcaca cggaggagca aatgaagcct gtatcaatat
    13741 gttgaaagaa atcggcactg tggatcgcat tcctgaattt attgctcgag ccaaagataa
    13801 aaatgacccg ttccgcttaa tgggctttgg gcatcgtgtc tataaaaact acgaccctcg
    13861 tgcgaaagta atgcgtgaaa cctgccacga agtgttaaaa gaactgaaca ttgataaccc
    13921 actatttgaa gtggcattgg aattagaacg tattgcgtta agcgaccctt atttcattga
    13981 gcataaactt tatccaaacg tagatttcta ctccggaatc gtgctaaaag ccatcggtat
    14041 tccaacctca atgtttaccg taatgtttgc cctagccaga accgttggct ggattgctca
    14101 ctggaaagag atgtatctcc aaggcaatat caaaatcgtt cgtccacgtc aaatctacac
    14161 cggacatact aaacgtgatt ttgagccaat ggataaatca taagattggc aaaaaatcga
    14221 aaaattttaa ccgcttgttt ccacaagcgg ttatattttg cataaaattt gcaaatcagg
    14281 ctaatttatt tagcagttgt gttctaacat cttgaccatt ttcatcgctt tttacagcca
    14341 taataaataa gccttttcct tgacttaatt tctcccaaag attgccaatt aaggcttttt
    14401 ctttagaatc ttcattactt tttaattgtt cccctttata ttccacaatt aaaattcgac
    14461 catcaataag ctctacaata aaatcagggt aaaaattctg atgagctaat ggtaaagaaa
    14521 accctctttt tacaggattt cttaaccaat catcagaaaa actcagttgc tgctgattaa
    14581 aatggtaaga aacccgttta tcttccacat caatttcaaa actttggcta ttttcttgta
    14641 atctaaatcc atcaagtttg gctggataat ccaataaatt ccaactttgg taatctaata
    14701 gcacttgtga atcagctaag tcgaattcgc cttgaatatt catacacagt tgaggtatta
    14761 ctttaaatat ttcccctttt tcggcaggag aagataatat ttcaactcta ttctggtgaa
    14821 tttgtaactg ttgctcaatt tgctcttttt tcttacctgt tgcagttcga atcaacgctt
    14881 tttctaactt ttcacttaaa ttacctttta cttgcactaa aatgtgacct tcttgctcgg
    14941 taattttaag ttgagaacgt tttgcttcgc ttaaatcaga catttcaggg agttgcgtta
    15001 atgctaaaac tgttgtcggt gtatagaata aatctccttc gtttccaatc caattttctt
    15061 gttgtgggat acgaagcatt tcagctactt ctaatgcttc aaaccccatt ccaatcaatt
    15121 tatcttgtaa attacgagcc gtttcgccga acgacttaga ggaaagcgga gcataagcac
    15181 gatttaaatc ttcaatttga cggtgtttag cataaggcat tcgcaatact cgccctaata
    15241 gctgctccgc ttccttgcta gaggaaacat tttgtactga gcaaaaaata taagcaaaag
    15301 ggcaatccca cccttctttt aatgcctcaa ccgtgatgat gtaattgatc ttgcattgtg
    15361 gattaaataa atcgatatta tctaattcgt gttgattacc tatcacaatg gcaatctctt
    15421 cttcatcaat ttttaactca tcaattaaat actgttttaa gacttctact gttactttgc
    15481 cgtttttcgg ctcagcttga aacaataaat cggacgaacg tactcgctct catactgtgc
    15541 tttttgtgct aatgctcctc tattgattac agccccatct attgcttgtt gccagttgtg
    15601 atgttcggtc agcacaatag gcaatttgat catctcttct gcttttagat ggcttgccga
    15661 aacgtgatac aacacattac ttcctgcttt tctatctgtg tttggcgtag cagtaaattc
    15721 taaaatagct gatggggaaa gttgagatag cacatcaaaa gagagtgaag tccgagcatt
    15781 atgtgcttca tcaacaatga ccaaaggctg ataagctcgc aacaaatttg caaaagaaca
    15841 cttaattttg cccgtttgtt tggaggttaa accgttctct tgtagatcag tagtggaaat
    15901 cttttctaaa tttgcctgaa tatggggtag taaacgctca aaatgagctg ttaatttttc
    15961 attaaaagca tagatttttc gcccatcttg attttctacc cgaaaatttt gaatagtaga
    16021 aacgattaca atcgccttat taccaaaatc ttgtgggcga agtaaatcaa aatcttcaga
    16081 ctcaataacc attacttctc ggttaaaagt tttatcaagt actgcacggt aagggtgttg
    16141 cggatttttt aatgcttctg tagtttgggt tttaattgta cgagaaggca ctggccacaa
    16201 ggtaaccggg aaatcgcaat ccaaatactg ctttgcaata cgagcaatac tatgcgaggc
    16261 taacaacgtt ttaccgccac cagttggaat tcgtacacaa acataaggca catcacgcaa
    16321 attactgtgt tcacttggga tcttgtaact atattcaggt tgaatttggc taaatgcggt
    16381 ttttgcatca gattgcaaac aagccgaaaa atagtggctg acacggttta atgcctcatt
    16441 ttggtaattt tttaatttca tttttttctc ctactattaa cgagacatta catcatatgg
    16501 aatctgttta aatgtaattt tcttttcagc taactgcact tctcctaaac ggcacgcttc
    16561 gccatatacc acaatatcca ttccctgttg aataaattca gcaaagtatg gtaactccgc
    16621 cattaatttt cgtgtcagaa cattaccact ctgcggacgt ttatcgccta aaattccgtt
    16681 atataacaaa tagtaggctt tgccgttgaa agtgccgaca agcggtgatt tttcgctgtt
    16741 tttttgcaaa gggaaatgat tttctaaata ccaaatatgg gcggctaaat cgtcaaaacg
    16801 aatgttcgga ttaagtgaac cgaatgcgtc aaatacttcc tcgcctaact cacaaaagcg
    16861 aaacgatcca ccaccttgcc aatttactgc ttttgaaata ccgccttgct caccttcaat
    16921 tacttttttc aaccgtggga caacatgggt tttagcatgt tcgccaatct caataccgat
    16981 ataacgacgg ttcattttat gagcaactgc tgcagttgta cctgagccaa gaaagctatc
    17041 gaggacgaga tcatatggtt cagtcgatgc ttctaataca aattagatta atttttcagg
    17101 tttaggataa tcaaatgaat cttctgcaat atcctcccca aataattcta gatgttcact
    17161 agcagagtct tcatttgttg ccatatcata atgagctctg ctaattaagt tatgaatttt
    17221 tttcttttcc gcacttcgct caaatactct ccttgg
//
LOCUS       DANLUA010000031        17192 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLUA010000031 DANLUA010000000
VERSION     DANLUA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..594)
                     /locus_tag="PXB44_000773"
     CDS             complement(<1..594)
                     /locus_tag="PXB44_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5281278.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQY"
     gene            complement(596..1615)
                     /gene="purR"
                     /locus_tag="PXB44_000774"
     CDS             complement(596..1615)
                     /gene="purR"
                     /locus_tag="PXB44_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL5281279.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(1751..2479)
                     /gene="cmoA"
                     /locus_tag="PXB44_000775"
     CDS             complement(1751..2479)
                     /gene="cmoA"
                     /locus_tag="PXB44_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL5281280.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(2489..3271)
                     /locus_tag="PXB44_000776"
     CDS             complement(2489..3271)
                     /locus_tag="PXB44_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5281281.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            3390..3956
                     /gene="seqA"
                     /locus_tag="PXB44_000777"
     CDS             3390..3956
                     /gene="seqA"
                     /locus_tag="PXB44_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL5281282.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            3966..5384
                     /gene="menE"
                     /locus_tag="PXB44_000778"
     CDS             3966..5384
                     /gene="menE"
                     /locus_tag="PXB44_000778"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL5281283.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            5377..8754
                     /gene="mscK"
                     /locus_tag="PXB44_000779"
     CDS             5377..8754
                     /gene="mscK"
                     /locus_tag="PXB44_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL5281284.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQVVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQVQTISDALQQKLQQQSFWVKSNNPID
                     LEWFSKFVDITHYQFIDIGKKLNFSNWRDNLLPASILVGILLLLAIFINRQKDKIKQK
                     LHQINNRLKIVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFVAITYISFENPQEVWTW
                     GLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSVWIIALLLNTSIF
                     TNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEEKKESKSILLTIV
                     KLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWIIIRNILYRAFSISSRRL
                     AYRRLQEKREAAEAKTRAKAEQNDENPDGVELPFELKEDSIAVSEVKNQMLKLTDFVL
                     WIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMESITLLNLLTAILIIVVM
                     VVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAFGTLGMSWSKLQW
                     LLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGTYSGTVSKIRIRATTLV
                     DFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSDLELVKRLLLQVAEENP
                     KVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIHQLFNEHNIDIAF
                     NQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
     gene            8757..9839
                     /gene="aroC"
                     /locus_tag="PXB44_000780"
     CDS             8757..9839
                     /gene="aroC"
                     /locus_tag="PXB44_000780"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL5281285.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            9858..10685
                     /gene="mepA"
                     /locus_tag="PXB44_000781"
     CDS             9858..10685
                     /gene="mepA"
                     /locus_tag="PXB44_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL5281286.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            10758..12677
                     /locus_tag="PXB44_000782"
     CDS             10758..12677
                     /locus_tag="PXB44_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5281287.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            12783..13109
                     /gene="secG"
                     /locus_tag="PXB44_000783"
     CDS             12783..13109
                     /gene="secG"
                     /locus_tag="PXB44_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL5281288.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            13134..13219
                     /locus_tag="PXB44_000784"
     tRNA            13134..13219
                     /locus_tag="PXB44_000784"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13168..13170,aa:Leu,seq:gag)
     gene            13422..14663
                     /locus_tag="PXB44_000785"
     CDS             13422..14663
                     /locus_tag="PXB44_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5281289.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(14822..16690)
                     /locus_tag="PXB44_000786"
     CDS             complement(14822..16690)
                     /locus_tag="PXB44_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL5281290.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(16701..17096)
                     /locus_tag="PXB44_000787"
     CDS             complement(16701..17096)
                     /locus_tag="PXB44_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5281291.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         5318 a         3587 c         3347 g         4940 t
ORIGIN      
        1 atactgcttt ttatttttat accatttcca gcctaaaaat agaataacaa tgcctaaaat
       61 ggcaaaaatg aggtgctgcc cattagctac ttgttctttt agccaatcca aattagatgc
      121 cccatagtcg cctaaataaa cccaaattgg cacagagata atagcggcac agaaatcaac
      181 taataaaaat tttacgaagc tgacttttct ggtaatacct gaaactaagt aaaccaccgc
      241 gcgcaagcca ggtaaaaaac gagccataaa aagtagtttc catccttgtt tttcaaaacg
      301 ttcttgcacg gttgcaaaac gttcaggtgt cgcaacattc ctgattaatg gaaatttgag
      361 aattttttcg ccatagattc tacctagcca gtacattgtg ctatcgccaa ctaatactcc
      421 taacatacta acaaccagca tccaatgcac attggtgtag cctaatcctg aaataacacc
      481 accggatact aacgtaatat cttcaggaat cggcacaccg aaaccacagg caagtagcac
      541 aagaaaaaca gcccaataac catagctact aaaaaattca atcaatgttt ccatattact
      601 ccttaatgtt taaattccga accgatttac gtttgaccaa ttcaggaaag aactctaata
      661 ctttaggctc ttttgcgttt tcgttatctt caatacgttc taataataat tctaatgctg
      721 ccgagcctaa gcgagatttt gactgatgga ttgtcgtaag aggcggtgca taaaagcgtg
      781 agctatgcac gttatcatac ccgatcactg aaatatcttg cggcacattc agcccttttt
      841 cggtaatagc tgaaattgcc cctagtgcca ttgagtcatt aaaacaaaaa accgcggttg
      901 gtaatttttc actgctctta agcaaattat tcatacactc ataccccccc tcaggttcaa
      961 aatcgccttc ttgaatccat tcttcacgaa tagacaaacc cgcctcagat agcgcttttt
     1021 taaaaccttc aaaacgggtt cttgcaattt ctttatctct attgccggta attacagcaa
     1081 tatcttgatg cccgttttca attaaatgct gagtcgcaat atagccacct tcaacaccgt
     1141 gatctaaaat aatatcactg cgttcatcac ttcgtcccca atccataatc accattggga
     1201 tattgctgtt ttcaaagata tcaaatgaat tagctgtata ttcggagcac atcaccaaaa
     1261 tgccatcaac ccgttttttg atgagcatat ctaaatgatt ttgaattttc tcaagtttgt
     1321 tttgagtatt gcacaaaaat agtgaatacc ctttgctata gcaaaattcc tctaccgcta
     1381 acacaatttc agcgaaaaac ggtgattcgc tggttgtaat gatcataccg atagatttag
     1441 tcgtattaat tttcaaactc cgcgctacag cactaggaga atagttcagc gctttaatag
     1501 cctcccaaac tgcttttgta gtgtcctctg ctacaaagcg ggttttgttt ataacgtgag
     1561 aaaccgttgt agtcgataca ccagcatatt ttgcgacatc ttttatcgtt gccatacctt
     1621 tttcctccta aataagtagt ggtgcattat aactgattct aactgacttt tattataaag
     1681 aaatagatag aatgcaagcg gttgcttttt atcaagaatt tgcaaaaaat tttacaaata
     1741 tcaccgcttg ttaggcttta attgcaatca ttgagccaaa attaaagcat tgaaaccaaa
     1801 gttcaacttg tgtaaaacca gcttcttgca gacgtttttt atgggtttca atactatctg
     1861 ttcgcatcac attttccaaa gcattacgct tttggctgac ttccaattca ctgtagccat
     1921 ttgctcgttt gaatgtgtga tgaaggtcga tcaatagctc gttcatttcc tcactctcaa
     1981 aggtaaattt ttccgataac actaaaatgc cattggaatt taatccttga taaatcttct
     2041 gtaataatgc taggcgatct tctcttggta aaaattgcaa ggtgaaatta agtaccacca
     2101 ttgaagcatt tttaatttca atgtggcgaa tatcatcaag taaaatcgtc accgggatct
     2161 cagagtgata agcacttaaa tgggttttac aacgctcaac catcggctga gaattatcta
     2221 ccccgataat ctgcacattc ttcgcattca cattacggcg aatagataaa atccctgcgc
     2281 ctcgtgaaca acctaaatca taaacatttg aattatctgt gacaaaacgc tccgccaaca
     2341 ttccaatagc agtaataata ttggaatagc ccggcacgga gcgttggatc atatccggaa
     2401 acacttcagc aaccgattca tcaaaagtaa aatcgcccaa tttctcaatc ggagcggaaa
     2461 ataatgtatc tctttgcatt tttctccctt atttttctaa aaatttattg attgctctca
     2521 caacagttcc tgttttttct gcgtgaaccc aatgatcagc gtttgccaca atgaaagatt
     2581 tggcctgcgg aaattgtgcc aaaatcgttt gtgtatgctc tgcttgaata taatccgata
     2641 atgcaccttt aataaacaag gtcggtttat ccacaaatac ctgctgccac cccatcacat
     2701 tctgataatt atcttttagc gcctgtaaat taaaacggaa agattgctcg ctttgggcat
     2761 caaaagattt caacataaac tgttgcacgc cttcatctcg aatatattca gccattatcg
     2821 ctttagcttg ctgacgattc tggggtttag cttctttcac cgcaaataag gctgccataa
     2881 cctgctgatg ccgattttgg gtataagtga ccggggcaat atcaatcact accaatttct
     2941 ccaccagttc aggagcaaat gcagccaatg ccatcgcact ttttccgccc attgaatgcc
     3001 caaccacaat agcattttgg atatttaatt ctgtgagtaa ttgtgctaaa tcttgtgcaa
     3061 tcaaagcata attcatttcg tctgaatgaa aagatcgccc gtgatttctg agatcaacca
     3121 ttaaaatgga gtaacgttct gcgaatggtt ttgctattgc gcctaggtta tttaaatctc
     3181 caaacaatcc atgtaagaag atcatcgtct gagcattagc atgattattc gaaggttggt
     3241 aattaaagtt taactgcgat ttttctgtca tataaatgtc attgttcata taaattttca
     3301 gcgatgttcc tctataaaaa acaatagaaa tgcgtataat agcaagcact attaacgcat
     3361 ttaatacccg cagatatttg aggatcccga tgaaacgaat cgaaattgat gatgaattat
     3421 atcagtatat tgcaagccgt acacaatcta ttggtgaaac tgcgtcagat atattacgcc
     3481 gtctattacg cctgccacaa tcgcctcagc cttttgtttt agtacaagaa cacatgatca
     3541 acgagcttaa agagcttgtc aaaacgcctt ctcgtgctac gaaaaaaaat gaatccaatg
     3601 cagaaaaaac cgttgaaaaa ttggaaaaca ttttaaattc cgagcatttt atgaacgaaa
     3661 ataaaaatgt agtgcgtttt actatgctat tagccgcatt atatcgagca aatccggagg
     3721 cttttgctaa agcaaccgaa aaagtgcgtg gtaatgaacg tatctacttt tcgcaaagtg
     3781 aagccgaaat tttagctact gggagtggag taaaagccaa gcagatccca gattcaccgt
     3841 tttgggtaat taccaataat aataccgcac gcaagggact tattctcaaa ggcgtaatgg
     3901 aatccatgaa agtaccaagc cagttaatcg aacgcattca agcgcttttt atctaattaa
     3961 ttgaaatgct catcaattct actcgctttc ctacccaatt ttgggctgct cattcagccc
     4021 aaaatgtggc gattatttgg gaaaaaggaa catcagacta tcttaaccac cttccatcaa
     4081 acatcacttg gtatagtttg aatcagttga ttgcgcaagc tattttcgtg ttgcaacaac
     4141 aaggtgctac tccgcacagt ttgattgcct attgtggttc tcatcggctc attggcttgc
     4201 tcacttattg tgctagcctt gctttggggg ctaagattct aatgcttaac cctgctcaaa
     4261 cagcaagtca acgacaagca gtcttagatg acaatggtgt tgagctcatt attaatgatg
     4321 ccgactttgc aaaattttca gcaaaaacga ccgcttgtaa tgccttccct acgattgatt
     4381 ttgaaaaacc agctacccta accctaacct caggctcaag cggtaaacct aaagcagtgg
     4441 tgcattctat agcggcccat ctctatagtg ccgaaggagt atgtgaatta atggcatttg
     4501 agcagccaaa ctcttggcta ctgagcttgc ctctgtttca cgtttccgga caaggcatta
     4561 tttggcgttg gttatttaaa ggtgcaacac tgtatgttca cgaagacaaa agtgattttt
     4621 ttctttcagc acaaaaagtg agccatctct ctttagtccc cacccaatta caacgctatt
     4681 taaaccaatt agattctatg gtcagccaaa aatgtttact gggtggatct atgcttccag
     4741 ccgaattgat tgctcaagca caacaatctg ggatgactac cttttcaggc tacggaatga
     4801 ctgagatggc atcaaccatt tgtgcagtag aaaatgattt aaacagtgtt ggttttccgt
     4861 taaaacatcg ggaactaaaa ttagaaaatg gtgaaatttg ggtaaaaggc aaatcactcg
     4921 gcttaggcta ttggcaaaaa aatgggcaaa ttcgaccgct tgtaaacaca caaggctggt
     4981 ttgccactaa agatcgtggc gaatggaatc acaaaaaaca gcttgtaata aaaggacgat
     5041 tggataatat gtttatttca ggaggggaaa atatccaacc tgaggaaatt gaacaactga
     5101 tttttcgttc tcagcttgtc gaacagatta tcgtgttgcc gattgaagat aagcaatttg
     5161 gacatcgacc agtggcgttc ttacaattca agcaatctga ttcaaaaaat gaactccata
     5221 aattaaaaat ttggctgtct gataaattag aaagattcaa acagcctgtt gcctattatt
     5281 tactggatat agaaaaatac caaaaacaag gcttaattaa actatcaaga acacaattac
     5341 aacaagattt aaaactcaaa gtattaaagg aaatctatgt ttagaccgat tttaaaaaca
     5401 tttgtgctta gtttaggctt aatgctaaat agttatgcat tagctgaatt gccaacccaa
     5461 caggtagtgc aaacgcagtt agatagcgcc aaaaaattag atcaagccga tgcggataat
     5521 aaggcattgg ttcaaacact tgaagatacg cttgctttct tagcccaaat tgataaacaa
     5581 aaagcagata atcaggcatt gaataaatct attcaagatt cccaaaaagc attaaaaaca
     5641 agccaacata atcttgaaaa attaaaagag gtgactattg caagtgtcga taatttaaca
     5701 caaaaagcga caacagattt acaaaaagag ttaacctcta cacaatctca gctggaaaca
     5761 gtacaacacg atttaagtat tattaataat aatttagttg cacaaaatac cgcaccggat
     5821 aaggctcaaa cggccttaac cgagatggtt aaacgtaaac aggaaattag tagccaactt
     5881 tcagcgacta acattaaaaa tgaactacag acaaagttag aagtagaatt agaattaata
     5941 gaattaaaaa acacttacaa tcaaatatta cttggtggca gcgaggattt atctgcgctt
     6001 tactctgctc aattagatga aaaaaagcaa gagcagcaaa atctacaagc agaaattgca
     6061 aacctgcaga ctgctatcaa caataaaatt gtagaagagt ctcagaacaa gctagatcaa
     6121 gcacaagcgg ttcaaaatca gcaaaataat gcaacgacga acccagttat cttgcgtgaa
     6181 ttggatataa ataccaaagt cacacaagag ttgctaaaac agaccaaaga tatgacgcag
     6241 ttatcacaag ataatttgcg aatcaaaagt gtgctagata acttacagca aacccaacgt
     6301 aatattgaag aacaaatcag ttctctgcaa ggtacgttgg tgctttctcg tattattaat
     6361 aagcaaaaac aatcattacc acaagatgaa atgatttccg gactatcaaa acaaattgca
     6421 gatttacgtg tacgtgtttt tgatatcact gaatttaaag actcttttgc ggacataaat
     6481 gcttatatca gccgcataga gcaagatgaa aaaacaacct ttacgagtaa agagaaagaa
     6541 caactgagta aaattttaca ggaacgttct gacactttaa ctgagatgat taaatcatta
     6601 aataatcagc tcaatttatt aatcaatatt gaactaaacc agcaacaagt gcagaccatt
     6661 agtgatgcgt tacagcaaaa actgcaacag caaagcttct gggttaaaag taacaacccc
     6721 attgatcttg aatggtttag taaatttgtc gatattaccc actatcaatt tattgatatt
     6781 ggaaaaaaac tcaatttttc aaattggcgg gataatttat tacctgcaag tattttagtc
     6841 ggaattttat tgctattagc catctttatt aatcgccaaa aagataaaat taaacaaaaa
     6901 ttacaccaaa ttaataatcg actaaaaatt gtcgccacag atagccaatg gaacacccct
     6961 cttgctattt tttggacatt agtgctttgc ctgccaagta cactaatgtt cttagccgga
     7021 tttgttgcta tcacttatat ttcatttgaa aatcctcagg aagtctggac ttggggatta
     7081 aaaatggcaa tttattggct gtattttgcc tttttagtgg cgatgctacg cccgcacggc
     7141 ataggacata ttcactttaa tatgccacaa aaaagtaatg aaatcttccg taatatttta
     7201 aaacgttcgg tatggatcat tgctttatta ctgaacacct ctatttttac aaacttagag
     7261 atgggaatat cttacgacat gcttggacaa acaatgacta tttttgtcct tatattaatg
     7321 atttggatga ttgtaccggc atttaggcaa gctatttcta cctatgagaa tgtcacaaca
     7381 gaagagaaaa aagaatctaa aagtatttta ctcactattg tcaaattagt gctactgctt
     7441 gcccctatca ttcttatcat tctgattgca ttgggctatt actatactgc actggtgatc
     7501 attgagcatt tagtagattc ttattttgcg atcattactt ggattattat tcgtaatatt
     7561 ctctatcgag cattctctat ttcatcacgc cgtctggctt acagacgttt acaagaaaaa
     7621 agagaagcag ctgaagccaa aacacgagct aaagcagaac aaaatgatga aaacccggac
     7681 ggtgtcgagc ttccttttga gctaaaagaa gactctattg ccgtaagcga agtcaagaac
     7741 caaatgttga aattaactga ttttgtgctt tggattggct tatttgtgct gctttactgg
     7801 gtttggtcgg acttaattac tgttgcactc tacctagacg gcgttacctt atggcaacaa
     7861 agtgttacaa cagaagccgg gattgtaatg gaatccatta ccctgctgaa tttactcact
     7921 gctattctga ttattgtggt aatggtggta ctcattcgga acttatccgg tttacttgaa
     7981 gccctgattt tctcgcactt caagttatcg caaggtacac catacacaat taccacactc
     8041 ttaacctatt tgattattgc gttaggggca atctttgcgt tcggtacatt agggatgtct
     8101 tggagcaagc tccaatggct actaactgca ctttctgtcg gtctgggttt tggtatgcaa
     8161 gagatctttg caaacttcgt ttcagggatt atcatcttgt ttgaacgtcc aatgcggatt
     8221 ggcgacacca ttaccatcgg cacttacagc ggtacggttt ccaaaattcg tattcgtgcc
     8281 acaaccttag tcgattttga cggcaaagaa gtgattgtac caaacaaagc attcgtaacc
     8341 gaacgcttgg tgaactgggc attaagcagc accgcaaccc gcattattgt tcaagtcggc
     8401 gtggcttacg ggtctgattt agaattagtc aaacggctgt tattacaagt ggcggaagaa
     8461 aatccgaaag tcttgaaaga tccacaacca agggcttatt tccttacctt tggtgcaagc
     8521 actttagatc acgaattaag agcctatgtg aacgatttat ctgatcgtaa cccaactatt
     8581 gatgcactaa atacacgcat ccaccagctc tttaatgaac ataatattga tattgcattt
     8641 aaccaattag atgtttatat caaaaacaac gatacacaag atgaaattaa agtagattca
     8701 aaacgctttg agtttgaacg accaaataac ataacagcaa aagaaaagga ataacaatgg
     8761 caggcaacag catcggacaa ttatttaaag tgaccacttt cggcgaatct cacggtattg
     8821 cattaggctg tatcgtagat ggcgtaccgc cgaatatggc tttaagcgaa gcagatttac
     8881 agccggattt agaccgccgt aaacccggta cttcacgcta caccacgcct cgccgtgaag
     8941 atgatgaagt gcaaatttta tcgggagtct ttgaaggcaa aacgacggga acaagcattg
     9001 gtttaatcat taaaaacggt gaccaacgct cacaagacta tggcgacatt gccgaaaaat
     9061 tccgcccggg gcacgcagat tatacctatc agcaaaaata cggcattcga gattaccgtg
     9121 gcggtgggcg ttcttctgct cgggaaaccg caatgcgggt tgccgccggg gcgattgcca
     9181 aaaaatattt gcgtgagcaa tttggcattg aagtgcgagg ctacctctca caaatcggca
     9241 atatcaaaat tgacccgaaa accgttgaaa atattgaaca aattgattgg cacgcagtca
     9301 atagtaatcc tttcttctgc cccgatcccg ttgcgattga aggctttgat gccttaattc
     9361 gtgagctgaa aaaagagggc gattcggtgg gggcaaaatt aaccgtgatt gcagagcgtg
     9421 tacctgtagg gttaggcgaa cctgtgtttg accgcctaga tgccgacctt gcccacgcct
     9481 taatgtcgat caacgccgtg aaagcggtgg aaattggcga tggttttgca gttgtggaac
     9541 aacgtggtag ccagcaccga gatgagatga cgccgcaagg ctttctctct aatcacgccg
     9601 gcggaatttt aggtggtatt agctccggtc agccgattat cgctcatatt gcgttaaagc
     9661 cgacctcaag cattactatt cccggcagaa gcgtgaacaa aaacaacgaa gccgttgaag
     9721 tgatcactaa aggcagacac gacccctgtg taggtattcg tgctgtgccg attgcagaag
     9781 cgatgatggc gattgtacta ctcgaccatc tgttaagatt taaggctcaa tgccgttaaa
     9841 cacataaaat tggaaaaatg aaattattaa aaacactttt agtctcaagc cttgctttta
     9901 cagctaccgc actgaatgca acgcaatggg agaaaatcaa aactccggtt caaggaaaag
     9961 cacaatctat cggcggttat agtaacggct gtattattgg ggcacagccc cttgcattaa
    10021 aaggcgaagg ctaccaagtg atccgctcgg ctcgtaaccg ctattacgga cacccacaac
    10081 tgctcgatta tctcactcac ttaggcaaaa aaaccaaagc tgccggtgtg ccgaccattt
    10141 taatcggcga tatgggaatg cctgccggtg ggcgttttgc ctccggtcac gcctcccatc
    10201 aaaccgggct ggatgctgat atttggttta agttcggacc gatggacgat gaaaccgccc
    10261 gcaatccagc agggcttgcc accattatgg taaaccgtag taccgagcgg gttgatgatt
    10321 cactctggaa tcaacaccat actaatttag taaaacttgc tgcaaccgat cctcgagtgg
    10381 atcgcatttt tgtcaatcct gccattaaag tgaaactctg ccaaaccgca ggcaatgatc
    10441 gtgcttggtt acgcaaaatt cgcccgtggt acggacacga ctcccatttc cacgtccgtt
    10501 taacctgccc ggctgatgcc gcaagttgtg aaaatcaagc ccttgtgcct gccggtgacg
    10561 gctgtggtga agagctttac tcgtggtttg agccgaaaga agcctccggc acaccaaaag
    10621 ccaaagtgct acccacagcg cctgttcaat gtcagatgat tttatctgaa caaggcttga
    10681 attaattgca aaataacaac cttttttgac cgcttgtcag cccacaagcg gttcattttc
    10741 ttaaataatt tacgattatg cgtttatttg ttgccgaaaa acccagcctt gcccgagcga
    10801 ttgccgatgt gttaccaaaa ccccatacca aaggagatgg ctttatccaa tgcggcgaac
    10861 acgacactgt tacttggtgt atcggtcatt tattggaaca ggctgaaccc gatgtctatg
    10921 atgagcgttt caaaatgtgg cgaatggaac atttgccgat tgtgcctgaa caatggaaat
    10981 tgattcccaa aaaagagacg ctcaagcagt ttaaagtggt ggaaaagctg attaaaaaag
    11041 ccgatgtttt agtcaatgcc ggagacccgg atcgagaagg gcagctgttg gtggacgaag
    11101 tttttggcta tttgaattta tctgctgaac gcaaaagcca aattcagcgt tgtttggtaa
    11161 gcgatctcaa cccgaatgcg gtgagccgag ccattgaaaa attgcaaaaa aacagcgatt
    11221 ttataccgct tgcaacctct gctctcgccc gagccagagc ggattggctg tatggtatca
    11281 atatgacccg agcctatacc ctacaggggc gttgggcagg ctataaaggc gtgttatcag
    11341 tcggacgtgt gcaaacgccg gtattagggc tgattgttcg ccgagatctg gagatcgagc
    11401 atttcgtgcc aaaggattat tttgaggtgt tggcacagat tgtcgtgccg gaaacccaag
    11461 agcgatttaa ggcgcagtgg caaccgagca aagcctgtga agattatcaa gatgaagatg
    11521 ggcgggtgct gtctcgtcca ttggcagaaa atgtggtaaa acggattacc gaccagccgg
    11581 caaccgtcac cgaatatcaa aataaaattg aaaaagaaac tgcaccgctg ccttattctc
    11641 tttcggtatt acaaatggaa gccgccggca gatatgggct atccgcccaa gcggtgttgg
    11701 atatttgcca aaagctgtat gaaacccata aactaattac ctaccctcgt tctgataatc
    11761 ggtatttgcc gaccgaacat ttcaacgaca gatttaaagt gatggcggcg attgctcatc
    11821 atttacctga atatcaacaa aaaccggaag tgattgatgc cactattcgc aatcgttgct
    11881 ggaatgataa agaggtggaa gcccaccacg ccattattcc cactgcacgg caagggacgg
    11941 tacatttaac cgaaaatgaa aaaagagttt atcaactcat tgcccgccaa tatctaatgc
    12001 aattctgccc tgatgcggaa tatcgcaaaa gtaaaatcgg gcttgaaatt gcaggtggta
    12061 gttttgtcgc tcaagcacgc aaccttgtaa ttgccggctg gcgagcgtta ctgggtaaag
    12121 aagatagcct tgacgatgca gagccactac taccgattgt caaaaaaggg caaatcctcc
    12181 attgtgaaaa aggggaaatt gtcagcaaaa aaacacaacc gccccgccac tttactgatc
    12241 gcagtttgct ttcagcaatg accgggatcg cccgttttgt acaagataaa gaattgaaaa
    12301 aaatcttgcg tgaaaccgat ggtttaggca cggaggcaac ccgagccggt attatcgaat
    12361 tgctgtttaa acgtggtttt ttaatccgta aagggaaaaa tatccacagt accgaagccg
    12421 gcagaatatt gattcaagca cttcccgact ctgctaccca gccggatatg accgctcatt
    12481 gggaaatgca gctgaatcaa atcagcaaaa aagagaccag ctatcagcaa tttatgtttg
    12541 atctaaacca acagctccca catttattac aaagcccaaa ccgccaagtg ttgcaaaatt
    12601 tagcgaaaat cacaccgctt gcaagcggca ataaacctcg ttattttaaa aagcgaacac
    12661 aaactgctga aaattaaatc aagcaggtaa aaattactgt ttagcacttg tcaaacgggc
    12721 gtaaattcta tattatgcac gccattgcaa atttcgcata aagaatacaa gagaataaat
    12781 taatgttaat taatgtactc actattgttt atcttgtagt tgccgtatta ctgatcgcat
    12841 ttgtattaat gcaacaaggt aaaggcgctg atgcaggtgc ttctttcggg gcgggtgctt
    12901 cagcaacagt tttcggctca gcaggatcag caaatttttt atcaagaaca actgctctac
    12961 ttgcaacgat tttcttcgca attagtttag ttatcggtaa tttaagctca cacaatgtag
    13021 caccgaaaag ccaatttgaa gacttaagcc aagttgaaca aaagcaagta gaacctgtaa
    13081 aaatagaaaa tagcgatatc cctcaataat cgcttaacat taaaaagaat aaagctctgg
    13141 tggtggaatt ggtagacacg ccatcttgag ggggtggtgg acacagtccg tgcgagttca
    13201 agtctcgccc agagcaccat tcaactaaat ttggctcaac ggagctaaac taaaaaagcc
    13261 ttagaaagcc tgtaaaatca acatttacag gcttttttac tatcttaact caacggctct
    13321 aaacgtttcc tatcacaaat tttagttaca cgtttagtta cacgttttaa atcgtgttac
    13381 tatactttca ataatcgtgt aacacttcgt aggtttaatt tatggctaga acaactaaag
    13441 cattaaataa aactcaaata gaaaatgcca aatcaaagga taaagaatat attttatccg
    13501 atggcgatgg attaaatctt agagttaaac ctctccctaa aggctctaaa atttggattt
    13561 ttaactacac taacccacaa actaaaaaca gaacaaattt aactattggg cgttacccct
    13621 taattacact tgcggaagct cgcactatat gtgatggcta ccgctcgtta atagagaaag
    13681 gcatagatcc acaggaagaa aaaaagcggc tgcaacaaga agcagaaaat cgagtaaatg
    13741 atactttccg gaatgtggcc gaaagctatt tcaatggtat ttataaagag aaagcaaaaa
    13801 atccgattac tcgtgaaaag aattggataa ggttagaaaa tcatattttc ccctatatcg
    13861 gggataaaca cgttgaagaa atcaaagtaa aagcccttgt tgatatttat tctaaagtgg
    13921 cagataaaag caacacatta aaaaaactgc atcagcttgt atctgctgta atggatcacg
    13981 ccattacgca aggcattata gaaagccata actgccgatt agcagttaaa aacttttata
    14041 tcaagccatc tacgccaaat cccactattg agcttgaaga attaccgcag ttatttactg
    14101 atttacataa tgcgaagtta tcaaagcaaa cttacgccct tgtttgttgg tcgttcttga
    14161 ctgctctacg cccaaatgaa gccgttaatg ctgaatggaa agaaatagac tttgagaaaa
    14221 agctatggaa tatccctaaa gagaagatga aaggaaaagc agataaaaaa cgccctcact
    14281 ctgtgccgtt atcctcccaa gcgttaaaga tattagagat gatgaaaatg cactctaatt
    14341 ctagcccttt tgtttttcca ggtcgttcat caaatagtaa accaatgagc aacgcaacgg
    14401 taaattcaac attaaaaagg aatggatata aagatagact aaccgcacac ggtattcggg
    14461 catttgtgaa aacatttcta tcttctcgca aagtagatcg ccacgtttcc gaaagtatat
    14521 tatcccattt attagaaggt ggagatgatt tagaaaacac atacaaccga tatgattttt
    14581 tagaagagcg tgtgccagta atgcagcttt taggtgatta ttgcgaacag tgcggaatga
    14641 atttagagtt agttcggggt tagggaggta aaatatatga ttttagtcgt cacgtcatca
    14701 cttttcccac tttccccttt aaaatcaaat agttaagtgg tgatgactgg ttttaaagtc
    14761 atcattaagt catcacaaaa aggtgatgac ctagaaaaaa agaggggttt ccccctcttt
    14821 gctaattcaa aatatcactt tgaaagtcat caaaattttt gaaatgaacg tttgttcgtt
    14881 gccctttatt cgtctttcgt tttgtgaaat cgtgcttatt gccgtgttgc tttactgctt
    14941 gctcaatacc tactacaaaa gtatttaagc ctagttctag cacattctta gccttaacaa
    15001 aggctaaata tgccggataa aggtgcgttc ttattttgtc gtgtccaagc gtgttgccag
    15061 ttccgatata aagcccgtct gtattttctg ttgtatatag atactcgaaa aaatccgtta
    15121 atgggtcaga ctgcttttta atctccaacg cctctaggct ctctttttgc tcctctaagg
    15181 ctcttttagc ttcgagtggg tcagggaagg tatcaagcac tttacggata atgcccccta
    15241 tctctaatgt aattttatcc ataaaatacg gatcacgttc attttcaggc ggtcttttat
    15301 taaaacggaa attgacaatc cgcctatcta taccgccatt ccgttctgtc catttacaag
    15361 ggtcgttatt gacgatcata atcattgccg caattcttgc gttaaatggg tgcttgttct
    15421 tcggatcgac tcgcaccata tcaccgccag taatcgcttt caatccgcca ccatctccag
    15481 cgtatttcgt ttgctcggaa cagataatca gctttttatc ttccagccct gcgattttgc
    15541 gttcatcatc aaagttttct agccttgctg aagctgtgtt cttttctcca gctaatagtg
    15601 tcgcaatgcc agcaaatacc gatttaccgc ttccgccttt ccctgttact tggaagaata
    15661 cttgccagtt gtagcggttt gttaaaatcg catagagagc ggctagaatg ttcttcgcct
    15721 tgtcttgctt gccgtctgat acaaagttaa gccattgatc aaagtgtggt gtttcggcta
    15781 cgttctcgtc atagctatgt ggaatataag ccgttagcca gttttggcgg ttaacggttc
    15841 tttgaacatt taccgaataa ggtgggtaag gggttaaagt ttcgggttgc cccttgctat
    15901 tccaaccaat caccgtagcc ggttgtactt ccattttttg aagcgtgccg aatggtcggg
    15961 ttttatcgtt ggctgtcgtg cgtggataag ctaagaagcc tttagcttgt tttgtgcctg
    16021 taccgttggt taaatcggtt tcttcagttt cgacaaagga ttctgaaatt tcttcagtca
    16081 gccagcctag aatatctaca ccaccaaagt ctaaaatctc ttgcgtggtt ttcgggtaag
    16141 cgtaaatatt atgaacggca atcgttactt cattcatttt cggggtggcg gtttcggtgc
    16201 gtgcttcgcc ttcaccgata tgatttaccg ctgcaccgcc agccgatact aattttttat
    16261 attctttgcc ttgggtcagt ttcaccacgt tagcaatttg tcgcataaca gaatcatctg
    16321 ttaggcgttt cattacttgc ttgtcaagtt ccggaatcac tgaatagcca ccgtctgccc
    16381 ctccatttgt attcgtagca agcgaacgca attcgcctgt tttaatccag tggcgaagct
    16441 cgtcatcaga tactttcaca tttgcggttt caacctgttg ccccggttgg ttacgctgtt
    16501 catcagtaat cgcttcataa cgtgcgattt cttttactaa attttcagct tgtgagcgga
    16561 gttcatcaaa cttagcccct tctgcttcag ttgttgagcg tttttcttcg tctgctttgg
    16621 ttaaaattga acgcatttct gcttcaatag ccgctttttg ttggcggagt tctaaaagtt
    16681 ttttaaacat tgccttatcc ttaattcaaa taattgatat tgtagtgaag cgttgctgtt
    16741 ttccacgctg attcgttagc atcataatca tagctatact tagccagcac acattcgtcc
    16801 atactgtgga atgtggcttg attcatcaca tcactgattt tttgagccac taaatctagg
    16861 tcgttttcgg ttgaaaacat tggtaagtaa atgccgattt tcaaataggc gttgcattcg
    16921 ccatcgcaaa gcgtgagttg gctatattcc gcttcatcaa tgaatacggc gatagccggt
    16981 aattcatttt ctacatcggt gaatacggct cggccgttat ggaaatgttg aatgccttga
    17041 atgttacctt gtaataagtc gataacttct tggcggattg cttcgttaga aatcataaaa
    17101 aagtcctctt aattaagaag gctacaatta gccacagaac aatataggca taatttcaac
    17161 ggagtaaata gggtaaaatt taatcgttta gc
//
LOCUS       DANLUA010000032        17125 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLUA010000032 DANLUA010000000
VERSION     DANLUA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17125
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            268..3132
                     /locus_tag="PXB44_000788"
     CDS             268..3132
                     /locus_tag="PXB44_000788"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL5281292.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(3203..3796)
                     /locus_tag="PXB44_000789"
     CDS             complement(3203..3796)
                     /locus_tag="PXB44_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5281293.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(3931..5385)
                     /gene="gnd"
                     /locus_tag="PXB44_000790"
     CDS             complement(3931..5385)
                     /gene="gnd"
                     /locus_tag="PXB44_000790"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL5281294.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            complement(5468..6157)
                     /locus_tag="PXB44_000791"
     CDS             complement(5468..6157)
                     /locus_tag="PXB44_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL5281295.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            complement(6167..7054)
                     /locus_tag="PXB44_000792"
     CDS             complement(6167..7054)
                     /locus_tag="PXB44_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL5281296.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            complement(7230..7928)
                     /gene="pgl"
                     /locus_tag="PXB44_000793"
     CDS             complement(7230..7928)
                     /gene="pgl"
                     /locus_tag="PXB44_000793"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL5281297.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            complement(8068..9555)
                     /locus_tag="PXB44_000794"
     CDS             complement(8068..9555)
                     /locus_tag="PXB44_000794"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL5281298.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            complement(9630..10448)
                     /gene="cysQ"
                     /locus_tag="PXB44_000795"
     CDS             complement(9630..10448)
                     /gene="cysQ"
                     /locus_tag="PXB44_000795"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL5281299.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(10462..11004)
                     /gene="nudE"
                     /locus_tag="PXB44_000796"
     CDS             complement(10462..11004)
                     /gene="nudE"
                     /locus_tag="PXB44_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL5281300.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            11092..11508
                     /gene="hslR"
                     /locus_tag="PXB44_000797"
     CDS             11092..11508
                     /gene="hslR"
                     /locus_tag="PXB44_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL5281301.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            11752..12645
                     /gene="hslO"
                     /locus_tag="PXB44_000798"
     CDS             11752..12645
                     /gene="hslO"
                     /locus_tag="PXB44_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL5281302.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(12719..13357)
                     /locus_tag="PXB44_000799"
     CDS             complement(12719..13357)
                     /locus_tag="PXB44_000799"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL5281303.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(13385..13771)
                     /locus_tag="PXB44_000800"
     CDS             complement(13385..13771)
                     /locus_tag="PXB44_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5281304.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(13768..14541)
                     /gene="hisF"
                     /locus_tag="PXB44_000801"
     CDS             complement(13768..14541)
                     /gene="hisF"
                     /locus_tag="PXB44_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL5281305.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(14523..15272)
                     /gene="hisA"
                     /locus_tag="PXB44_000802"
     CDS             complement(14523..15272)
                     /gene="hisA"
                     /locus_tag="PXB44_000802"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL5281306.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(15442..16287)
                     /locus_tag="PXB44_000803"
     CDS             complement(15442..16287)
                     /locus_tag="PXB44_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL5281307.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(16386..16979)
                     /gene="hisH"
                     /locus_tag="PXB44_000804"
     CDS             complement(16386..16979)
                     /gene="hisH"
                     /locus_tag="PXB44_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL5281308.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         4742 a         3968 c         3557 g         4858 t
ORIGIN      
        1 cggtatattt cctccgacaa tcaaattaat caatttttca tccaatatag aagatgatat
       61 taaagagatt gagcaaagct gtttaaatag ccgaaagcaa ttatgggaaa aacgaagaaa
      121 aatgcaagag gaacaaaaaa ttcccaatta atcttttagt tgaccttaat ttaaataact
      181 aattacctat aaaagcattt acggcaaaga tcttttttat aagattaagc tagtgaaaaa
      241 attattaaac aagagaaaac aaaaccaatg acaaaacaat tcacaatgga agaccgtttt
      301 aacccttccg ctgtagagca agcgctctac caacactggg aatcgcaagg ctattttaag
      361 ccgagtgaag atattaatgc accgagctac tgtatcgcta ttccgccgcc gaatgtgacg
      421 gggagtttgc atatggggca cgctttccag caaaccttga tggatacgct gattcgtttt
      481 aaccgtatgg agggcaacaa taccctttgg caaacaggaa ctgaccacgc agggattgcc
      541 actcagatgg tggtggagcg taaaatcgca gcggaagaag gtaaaacccg ccacgattat
      601 gggcgtgaag cctttatcaa caaaatctgg gattggaaag cctattcggg cggcacgatc
      661 agccagcaaa tgcgtcgctt aggcaactcg attgactggg atcgtgagcg ttttacgatg
      721 gacgaaggct tatccaatgc ggtaaaagag gtgttcgttc gcttgcacga agaaggcttg
      781 atttaccgtg gcaaacgcct cgtaaactgg gatccgaaac tgcatactgc gatttctgac
      841 ttggaagtgg aaaacaaaga aagcaaaggc tcgctgtggc atttccgcta tccgctggct
      901 aatggtgcaa aaaccgcaga cggcttggat tacttagtgg tggcaacgac ccgtcctgaa
      961 acgatgttgg gcgatactgc ggtggcggtt caccctgaag atgagcgtta tcaagctctg
     1021 attggcaaaa gcgtaatcct accgcttgcc aaccgtgaaa tcccgattat tgccgatgag
     1081 tatgtggatc gtgagttcgg tacgggcgtg gtaaaaatca cccctgccca cgatttcaac
     1141 gactacgaag tgggcaaacg ccacggcttg ccgatggtga atgtgatgac cttaaatgcc
     1201 gatattcgtg cagaggccga aattatcggc acagacggta aaccgctttc cgactacacc
     1261 gccccaattc ctgccgacta tcaaggctta gagcgttttg ccgcgcgtaa gaaaattgtg
     1321 gcagattttg aagctcttgg gctgttagat caaatcaaac cacacgactt aaaagtgccg
     1381 tatggcgacc gtggtggcgt gccgattgag ccgatgctga ccgaccaatg gtatgtgtct
     1441 gtcaaacctt tggcggaagt cgcgaccaaa gcggtggaag acggcgaaat ccaattcgtg
     1501 ccgaaacagt acgaaaacct gtatttctct tggatgcgtg atattcaaga ctggtgtatc
     1561 tctcgccaat tatggtgggg acaccgcatt ccagcgtggt atgacgagca gggcaatgtt
     1621 tatgtagcac gagatgaagc ggaagtgcgt gcgaaacaca acttacctgc ggacttacca
     1681 ttaaagcaag atgaagacgt actggatact tggttctcgt ctggtctatg gacgttctca
     1741 acgttaggct ggccagaaca aaccaaagaa ctgaaaatgt tccacccgac cgatgtgctg
     1801 attaccggtt ttgacatcat cttcttctgg gtggctcgta tgattatgtt cacgatgcac
     1861 ttcatcaaag atgaaaacgg caaaccgcaa gtgccgttca aaaccgtgta tgtaacaggc
     1921 ttgatccgtg acgagcaagg gcagaaaatg tcgaaatcga aagggaacgt gttagacccg
     1981 attgatatga ttgacggcat cagtctcgac gatttgctcg aaaaacgtac aggtaatatg
     2041 atgcagccac aactggcaga aaaaatcgct aaggcaaccc gcaaagaatt tgccgaaggg
     2101 attgtggcac acggcacaga cgctctgcgt ttcaccctca ccgcccttgc cagcaacggt
     2161 cgagacatca actgggatat gaaacgttta gaaggctacc gcaacttctg caacaaactg
     2221 tggaatgcga gccgttatgt gctgaccaat gacaagttgg atttaagcga aggcgacgtg
     2281 gaatttagcc ttgccgatcg ttggattgaa agccaattca accgcacggt ggaaactttc
     2341 cgcaccgcac tcagccaata ccgtttcgac ttagtggcaa atgcgatcta cgaatttact
     2401 tgggatcaat tctgcgactg gtatttagag ctgaccaaac ctattttctt caaaggcaca
     2461 gatgtacaac gccgtggcgc aagtcgcacg ctggtgaatg tattggaaaa actcctgcgt
     2521 ttaatccacc cagtaatgcc gttcatcacc gaagaaatct ggcagaaagt gaaaggcttt
     2581 gtaggtattg aagccgatac cattatgttg caaaaattcc cacaatttga cccgcttgcc
     2641 attgatgaaa ccgccgaaag tcaaatcaac tttatcaaag aagtgattgt ggcggtgcgt
     2701 aacattcgtg cggaaagcaa catcgcacca agcaaaggtt tagatttaat cgctcgtaat
     2761 ttctctgcgg acgaagtctc tattctcaac gctaacgaag tgttgctcaa atcgatggca
     2821 aaacttgata gcgtgaaagt gctggaaaat ggtgaaaatg caccgctatc ggtagcgaaa
     2881 ctggtcgcaa atggtgagat cttgatccca atggcaggct ttatcaacaa agaagctgag
     2941 ctagctcgcc tcaccaaaga gatggataaa cttaaaggtg aagtggctcg cattgaaggc
     3001 aaactttcaa acgaagcctt tgtcgccaaa gccccagagc aagtcatcgc caaagagcgt
     3061 gaaaaaatgc aagagtacct gagtggatta gagaaattgc aggtgcagta tcaagagatt
     3121 gaagcactgt aattaactaa caagcggtca ggatttggaa attttttgct aattcagacc
     3181 gcttgcttat tgaagctttt cattataaat gtttggttaa aatcgcctct aggcggtcat
     3241 agcctgcttg ggtgaagtgt aatccatcgt ggcataaagt tagatctaaa ttcccttctt
     3301 tgtcagcaaa atattcccac gtttttacat aagtcaaatc agatggaata tttgcctcaa
     3361 aatacgcatt gagctctttt attgcagcat tattggtggt ggtaatatta ttaaccggtg
     3421 tggcttccaa tagaaaaaag ttggcatttg gattaatttt gcgaagagaa tccacaattt
     3481 cgccaatcca ctctaacact tgttttggcg agtaatccgg ctcttttaca atgtcattaa
     3541 cgcctagaaa aataaataca ttatcgccaa cgtgttgaat tcgctcttgt cgggtaatca
     3601 catccaaata ttggcgggtg ctggtgcctg ataagcccaa attggcaact gtttttcctg
     3661 ctaaattcgg cgtgccgttt tccatatcgc cccacatatc aaacagggaa tgcccaataa
     3721 gggaaatatc tgcctgtttt tcaaattctg atttttttgt ttggtagcga ttaaaaatat
     3781 cttgatcggt aagcataggt tctccttaaa tataaatgaa aaatcgctcc attattatac
     3841 aaacttgtaa acattcactc caagatattt tgaagaaaaa tccaataaaa aagccactta
     3901 atatcgaatt aagtggctca aaaagattaa ttacacatca taagtggtcg atgcggtatt
     3961 accgccacgt cctgtccagt tggtgtggaa gaactcaccg cgagctttgt ctgtacgctc
     4021 ataagtatga gcaccaaagt agtcacgttg tgcttggagt aagtttgccg gtaaacgtgc
     4081 cgaggtgtag ccatctaaga aggtaatggc tgacgccata caaggtacag gtaaaccgat
     4141 ttccaccgat tttgccacta ctttacgcca atctgccaag cagttttcca aaatatcttt
     4201 gaagtatggg tctgagccta agaacactaa atatgggttg gcttcatacg catcacgaat
     4261 gttgcctaag aaacggctgc ggataataca accttcacgc cataataggg cggtattgcc
     4321 gtagttgatg tcccaaccga attgctcaga agcctcacga atcaacataa agccttgtgc
     4381 ataagagatg attttggaag caagtaacgc acggcgtacc gcttctaccc atactttttt
     4441 gtcgccttca actttaccaa tttctttgtt aaacaatctt gaagccgcaa cacgttgatc
     4501 tttaaacgca gatacgcaac gggcgaatac cgactcggta atcagtgtta atggaatgcc
     4561 gaaatcaagg gcgttaatac cagtccattt acccgttcct ttttgacctg cggtgtcgag
     4621 aattttctct accaatggct cgccgtcttc gtctttgtag gcaagaatat cagtggtaat
     4681 atccaccaaa tagctgtcta attcggtgtt tttccactct ttaaagattt cgtgcatttc
     4741 atcatagctt aaacctaaac catcttttaa gaattggtag gcttcacaaa ttaactgcat
     4801 atcgccatat tcgataccgt tgtgaaccat tttcacaaaa tgccctgctc cgtcacgacc
     4861 aacccagtca caacaaggct cacctttgtc ggttttggct gaaattgctt ggaaaatcgg
     4921 cttcacatat tgccacgcag attcatcacc gcccggcata attgacggcc cgtgacgagc
     4981 accctcttcg ccgcccgata cacctgagcc gataaagcga atgccttttt ccgctaacgc
     5041 tttcacacgg cggttggtgt ccggatagtt tgagttaccg ccatcaatga tgatgtcgcc
     5101 ttcttctaaa tgtggcagaa gagcctcgat aaactgatcc accacctcac cggcacgcac
     5161 cattagcatc actttacgcg gtttttccaa cttcgccgct aaatcttcta acgagtaagc
     5221 accgataatg ttcgtgcctt tcgccgcacc ttctaagaac tcatccactt ttgaagtagt
     5281 gcggttgtac gccaccactt taaagccgtt gtcattcata ttcaaaatta aattttgacc
     5341 cattacggcg agtccaatca cgccgatatc gcctttttgt gacattgttt gctcctattg
     5401 tttttgattc tatgcaagcg gtcgaattgg taaaattttt tgcaaatttg accgcttgta
     5461 acatttatta acctaaaata aactccaccg ccatttggct gtgtaatgct gctgtatcga
     5521 aaaacggtaa cggattgtcg ttttggttaa tcagcaaccc gatttcggta caacctaaaa
     5581 tcacaccttc tgcaccttgt tcactaagtt gttgaatagt ctgaagataa aacgctttgg
     5641 cactttcagt aatcttgcct aaacagagtt cctcgaaaat aatgcgatga atttcttgtt
     5701 gcgtttcctc atctggtact attgatgtga taccacgctc caataaaccg tctttgtaaa
     5761 aattatcact catagtaaat tttgtgccaa ggagtgctac tttctgcaga ccttgagctg
     5821 aaatcgcctc agccacacag tccaaaatat gcaaaaacgg cacagagata tggctactaa
     5881 tttgatcggc aattttgtgc attgtgttgg ttgccaataa aatgccttct gctccggctt
     5941 gttctagcaa tctagcttgt tcagataaaa tttcagcggc tgcttgccaa tttccctgct
     6001 tttggcattg tacaatctct tcaaaattga cactcgaaat taaaatcttg gcactgctat
     6061 ttccacctaa tactttattc accgagcgat taatatctaa ataataagaa accgtgcttt
     6121 ccgcactcat tccacctaaa atgccgagtg ttttcattaa tataccttat tgattttccg
     6181 ctaacttttc taaaatgcgg ctcattgata aatccatttt agaaaaagcg aaccatttct
     6241 tacctaaatc tttaagtgat gcaccgatat gataaagctc tttttcatca ataatcaaaa
     6301 atcgctcgtg agatttatca aatattttca cattaatgtg agcatattgg ctgttatatt
     6361 tttccaaatc taatgcaagt tgcttagata tttgtttcgt ataaatcgta actgatacgt
     6421 tatgcgatcg tttcgtaaat aaagttaaaa cggtatcatc tacatagtta tcaattaaaa
     6481 tcagcgatga ttttgcacct ctcactaatt ccgtaacaaa ctgataagcg ttaaaggttt
     6541 caccttcaaa gaaaacccct tgcattttag gcttattgcc ttcaagagat tgaaacacct
     6601 gctctaactg cccagacatc tccaactgtt tacgctctac actttctaag cgttgtacca
     6661 aaccactatt tgtttgcaaa agatgacgca tttgcacaaa ggcttgcata atcttaatgc
     6721 taacttgcac cgcaacttca ctacgcaata ctgccgaaag cattgcaatg ccttgctcgg
     6781 taaagacata aggaagatat ttcctatgtt ggccacgccc actattttcg tttaagatca
     6841 caaattgtga tcttaaagat agccattcgt tttctgttaa ttgaaaacga aatatgtcag
     6901 gaaatcgctc ttcattccgt tttactgctt ggttaaaggt tcgagtatct acctgataaa
     6961 gctctgccaa atgaacatct agcattactt gctcgccacg aatagtaaaa attttagagt
     7021 taatgtttgg ttcaattaat tgaattggat tcatctttct tccttattct tcagtgaaga
     7081 tcacttttgt gatctcaaaa taagaaagac tatgccaatt tttggcctat accacgagca
     7141 gatttttctg ctttttcgat gaatatcaca aaaatacttt gcaacatttt tctaaaattt
     7201 aaccgcttgt aaaggtcact gattgtgacc tagagtaagt tagccgcctc actatccaaa
     7261 taccattccg ttaccccatt ttccgcctga attttggcgg caggataagg taaattttct
     7321 gccggagtgg tttggatttc ttttaaaatt tccgctttgc ttgctcctgt gaccaagtaa
     7381 gtgatccgtt tggctttttc aattaaagat gccgttttgg aaatgcggat ctgccctgtt
     7441 tctgggtatt tggcgatcac ggcaacgttc tgatcattaa aatctgtttg atgtgggaac
     7501 agtgaggcgg tatgcccgtc tgttcccatt cctaaaataa tccaatcaaa cgccaagttc
     7561 ggcacacaag cggtcaattc tgccgaaaat cttgcaagtt cttgttctac attctcttca
     7621 ccacgaatgc ggtgaatatt ttcgcttgga atgtcgatgt gatcgaacag taatttctgc
     7681 acttcgccgt agttactttc gggatcggtt ggggcaacca ttcgatcatc cccccaccag
     7741 aaatgcaggt tttgccattg aatttcggta ttaaacggtg cttgagctaa ggttttgaat
     7801 aataatttgg gggttgaacc accggaaagg gaaatatgca ccgggcggtt ttgatggctg
     7861 tattcaataa actctttagc aatttgttca accgcagctt gggcggtggg gaaaataact
     7921 ttattcataa tttgcctcgt tagatcaccc ctctttagaa aagaggggag ggggagattt
     7981 gattacgatt tcaatatttc gtatgagcgt atataacttc tgccaaatct cccctagccc
     8041 ctctttgcta aagaggggaa ctgaaattta cactttcttc ttcattgtac cggacggttt
     8101 tcgccatact ttgccgtgtc tggcgattaa tttatctgcc tcggttggcc cccaagtgcc
     8161 ggattcatat tcatacacac gcccacgatt agctttgtag gctaaaatcg gttgcacaaa
     8221 tttccaacaa gcgtgtacag catcagtacg ggcaaataag gttgcatcac ctttaagtgc
     8281 gtctaatagt aagcgttcat aagcggtgag taagcttgat gatgaactta aatcagaata
     8341 gcggaaatcc attgagactt ctttcgcttc aaagcctgct cccggttttt tcaagccgaa
     8401 acgcattgaa atgccttcgt ccggttgcac acgaataatc aatttatttt ccggagcatt
     8461 ttggctaaat accgggtgtg gtgtagtttt aaaatgaatt acgatttccg tcacacgggt
     8521 tggtaagcgt ttaccggttc gcacatagaa cggcacacct gcccaacgcc agttatcgat
     8581 tttacatttt accgccataa aggtttcagt gttggaatca gccggcacgc ctttttcttc
     8641 caagtaagcc ggcacttctt tgccgtctac cgtgccacga gcatactgac ctaacaccac
     8701 attgtgttta acgtcttctt cagttaacgg atgcaagcaa tatagcactt ttgcgacttc
     8761 atcacgcatt gaattagcat taataattgc cggtggctcc attgcaacca tggctaatac
     8821 ttgcaataag tggttttgga acatatcacg cattgccccc gagccatcat agtaaccgcc
     8881 acgatcttca acgccgatgg attctgcccc ggtaatttcc acataatcaa taaaattacg
     8941 gttccaaagc ggttcaaata agccgttaga aaaacgtaaa acgagtaagt tttgtaccgt
     9001 ttctttacct aaatagtggt cgatacggta gatttgatgc tcttcaaagc atttgtggat
     9061 ttgtacgtct aattttttgg cggtttcgat atcgtagccg aatggttttt caacaataat
     9121 ccgtttccag ccaaactctt cggtagttaa gccgtgagcg gctaaacatt ccggaatcac
     9181 gccatataaa cttggtggcg tggagaggta ataaagcgtg ttaccggctg tttgatattt
     9241 atcgtgtagc tcatcaagac gaggcagcaa cttggcataa tccactgcat cagaggtatt
     9301 tacagcttgg taataaagat gttcgcaaaa tttatctaat tcttcaccgc ttgctttttc
     9361 aaatttgact aacgcatcac gcattttttg gcggaaagat tcgtcggtta attctgaacg
     9421 agaaacacct aggacggaaa aatgctcgcc caatctgcca atcttatata agttataaag
     9481 agcaggaatt aatttacgaa aagttaagtc accggaagca ccaaaaatga cgatacagct
     9541 attttcagca ttcatttatt tatcctattg ctaattgtta aaattttgtt gggtgcaaga
     9601 ttatcataaa aattgctaat cggaactacc tatttgaata aaaatatttt atcccaatct
     9661 atcgagccgt cagccaccat cacaaaataa ggattaatca aactttctcg ctgattataa
     9721 gtcagcggcg aaaaattaaa gtcgaaaatt ttgccgccca tttcacttaa cagcacttct
     9781 gccacagcag tatcccattc gccggtgtca ccaaaacgga tataacaatc ggctttccct
     9841 tctgccacta aaccggcttt tagactgccc gaaccatatt ttacacactc aatttgtggg
     9901 gctgaaatgg catttttagc ttgttctgca tgtaaatcgc tgcctacggt aacaatcaaa
     9961 agggcattct ccgcacgttt cggtttgaca agcggtcgaa tttctccatt ttcttgcaaa
    10021 aagctaccgc taccttccat actaaaatag gtttttccta atatcggagc atgaataacg
    10081 cccagcaccg gctgatttct accctcacgt ttttgcacta acgcaatcac cactgaaaat
    10141 tgaccggtgc ggttaataaa ctgttgtgtg ccgtcaatcg ggtcgataat ccaatattct
    10201 tgccattgtt ggcgttcggc aaacggaata tcgcaaaact cttccgataa taccggaata
    10261 tctggtgtga gttgcttcaa gcgttcagtt aaaaattggc tgataatcag atccgcttcc
    10321 gtcaccggtg tgcaatcggc tttgatttta attgccaccg agcgggcata aagctgcttt
    10381 aactgctcac cggctgcttt ggcaatattg ataatattct cgagtagggg ttgagttagc
    10441 gtttgcataa tagctccttg cctatttttt taagtattct cgtagcataa atagagcgga
    10501 caaattccgt gcttcggcaa aatcaggatc tgccaataat tgatcaattt tatccaacgg
    10561 ataacgcaca atttccagcg gttcaggctc atcgccttcc aattttgatg ggtacagatc
    10621 ttgtgccaaa aacaggtgca ttaagccgta catatggctt ggcgaactgt aaagcgaccg
    10681 cagaaaatct aattttttcg ccccaaaacc aatttcttct tgtaattcac ggttcgcact
    10741 ctcaatcggc tcttcacctg catccacaat gcctttcgga aaacccagct cgtaacgctc
    10801 cgagccgacc gcatactctt tcacaaaaat cagctcgttg ttttgaatcg gaatcaccat
    10861 taccgaagaa cggcgattcg gcgtaagtcg ttcgtaagtt cgctcttcgc cattagaaaa
    10921 gcgtaaatcg accgcttgca cctcaaaaat gcgagatttc gctactgttt taatgttcaa
    10981 aatttcagga agttgtttta tcattttatc tcaccttttt gagttatttt agttgaaaaa
    11041 tctcactttc gctctaatat ttcgactatt gttttgattt taaggcagaa aatgaaaaat
    11101 aaacacgaaa aagaacaaaa cgacgccgaa gttcggctag ataaatggct ttgggcggct
    11161 cgtttttata aaacccgttc gattgccaaa gcaatgattg aagggggtaa agtccattat
    11221 aatgggcaaa gagctaaagt tagcaaagcg gtggagatcg gtgccatcat caaacttcgc
    11281 caaggcaatg aagaaaaaga ggtggaagtg ttagccctca gtgaccaacg ccgaggggct
    11341 ccggaagccc aactgctcta cagtgaaacc gaaaaaagca ttaaacaccg tgaggcaatg
    11401 gcatttgccc gcaaagcgaa tgctctttca atgccacacc ctgatcgccg tccaaacaaa
    11461 aaagaacggc gggatttaat caaattcaaa gagcaagaat ttagctaagc ccttgcaatc
    11521 agaaaaataa tccctatatt gaatatccta agtaggggcg aaaaattttt cgcccctata
    11581 taattatttc gggcgaacat tgattcgccc ctacagcgtt cgatttgcac atttttagcc
    11641 acaaaatgca atgcattttg aacgttggct ttgccaacgg ctccgtagga gcgaacaaaa
    11701 ttagaaataa ttttgtgagt aataatcgac cgcttgcaac gaggaatcaa aatgaattac
    11761 accaaagaca atgacaaact ctaccgctat ttattcaaag accgagcggt acggggcgaa
    11821 tgggtgcgtt taaacaacac ctttaccgag accttaaaca cccatcacta ccctaaagca
    11881 gtacaaaatt tactcggaga aatgttagtc gcaaccagtt tgctgactgc aacaatgaaa
    11941 tttaacggca acattactgt gcagattcaa ggtgacagcc cgttaaaatt ggcggttgtg
    12001 aacggtaacg accaacagca actccgtgct ttagcccgtg ttcaggcaga tattgcagac
    12061 acagcctcgt tagcggaaat gatcggcaat ggtgtattag tgatttctat tatcccaacc
    12121 gatggtgaac gttatcaagg ggtgattgct ctcgataaac caaccattcg tgaatgttta
    12181 gaagattact tcgcccgctc cgagcaatta caaacccacc tcgttatccg cacaggcgaa
    12241 tttgaaggca aagcagtggc aggcggtatg ttgctacaaa ttatgccgga cggcacaggc
    12301 tcaagagatg attttgagca tttagccacg ctcactgaaa ccgtaaaaga tgaagagtta
    12361 tttggtttag aggcagaaga attgctctat cgcctctacc acgaagagca agtagaagtt
    12421 tacccaccac aagatactga atttaaatgt ggctgctcaa gagagcgttc aggctccgca
    12481 ttattactgg tgccggcaga agaaatcgaa gaaatgttgg aggaaaaaca aggcgtgatc
    12541 gatatgcaat gtgaatgctg cgggacacaa tatttctttg acaaagcggc gattgaaggc
    12601 tttaaggaag aaactgaaaa attaagtaag ttagggttaa attaagctaa acaagcggtc
    12661 aagtttcttg tcttttttgc aatttgtaaa aaaacggtga aatttgaccg cttgcatttt
    12721 acttatttga gccttctggg tgtaacccaa tccctttgtg gcgttctttt agtttagcat
    12781 tgatctctgc ccacgagaga tcggcattgt gcaataacac cgttaagtga tacatcagat
    12841 cagccacttc accgaccaac tcttcccgat catttttcat tgccgcaatc acgctttcta
    12901 ctgcctcttc ccctactttt tggacgattt ttggcgtgcc tttggcgtaa agtttagcgg
    12961 tataagaact ttccggatca gcatttttac gttcggctat cattcgttct aatttgctga
    13021 aaaagatcca atctggttgt tcttgttcaa actgatgaaa acagctctcc gcccccgtat
    13081 ggcaggtttc gccgataggg ttagcgagga ttaacagggt gtcgttatca caatccaaac
    13141 tcatatccac cacgtttagg aaattgcccg aggtttcgcc tttcgtccac agccgttgtt
    13201 tagtacggga gtaaaaagtc actcgctttt cagccagcgt tttttcgagg gcttgttggt
    13261 tcatatatcc taacatcagt acttcgcagg tttgggcgtt ttggacgata acgggcagta
    13321 agccgtccac tttttgccag tctattttat tttgcatttt ttcttccttt ttttgaccgc
    13381 ttgtttagac atataggctt atttcaatca aattaccatc agggtcacgg caataaaccg
    13441 actcaatttt tcccactgcc cccgtacgag gcacaatccc accttccaaa atctccacct
    13501 gatgtcgctg taacacatag ataacctcct gaagtggagt taccgttaag aggcataaat
    13561 ctgccgtgcc acaatcggca ttttgggcat tgggtaggat ttctgcacct ttttggtgga
    13621 ggttgatttt ctgtgtgcca aacaaaagcg ctttgcgatt atcgccaaag gtgatttctt
    13681 gcatacccaa aatggtcgta taaaaatcaa tggttttttg catatcggca acggttaaaa
    13741 ccagatgatc taaggcagta atcttaatca tctacgcacc tcaacccctt ccttcgccaa
    13801 atattccttc aactccccaa tattgataat ttgtttatga aacacactcg ctgccaacgc
    13861 accatccaca tttgcctcaa taaaagcatc acggaaatgc accatttcgc ctgcaccgcc
    13921 tgaggcgatg agtgggactt ggcaaacttc acgcactttt ttcagttgca ccaaatcgta
    13981 gccgttgcgt acgccgtctt ggttcatcat attcagcacg atttcgcctg ctcctcgctt
    14041 ttgcacttcc tccacccaat ccaacaattg ccattcggtt tggcgggtgc gtttttcatc
    14101 gccggtatat tgattgaccc aatatttgcc ggtttctgcc tcgaaccagc tatcaatacc
    14161 aaccaccacc gcctgcacgc caaagcgctt tgccagttcg gtaatcagct cggggttggc
    14221 aagggctggg gaattgatgg agattttgtc cgctccgaaa gcgaaaatct gctccgcatc
    14281 ggcaatggtt ttgattccgc ctgccacaca gaacggaata tcaatcacct gtgccacacg
    14341 ttccacccag cttttatcca ccgttctgct gtcacttgag gcggtaatat cgtaaaacac
    14401 caactcatcc gccccctctt gggcataacg ctgggcgagt ggcacaatgt cgccgatgat
    14461 ttcgtggttg cggaactgca cgcctttcac aacttgcccg tcacgcacgt ctaaacaagg
    14521 aattatccgt tttgccaaca ttcgattgcc tccgctacat taaatttacc ttccaacaac
    14581 gcacgaccga caatcacgcc agccacgccc gtaccacaaa gcgctttgat gtcggcaagc
    14641 gagccgatac cgcctgagga ctgaaattga atctctgggt atttggcaca gatttctcga
    14701 tagagatcta catttgaacc tgccagcgtg ccgtctcggg aaatgtcggt gcataaaacg
    14761 tgttgcaagc ccacggtttg ataatcttca atcaactcct cgagcgacac accacttgcc
    14821 tcttgccagc cgctcatcgc aatgattttt tgaccgattg catcaatgtt cacatccagt
    14881 gccagcacaa atttctccgc accgtacttt ttgaaccagc ctttaaccat ttcacgctct
    14941 tttaccgcag tcgaaccaat caccacacgg tttgctccaa cagcaagtaa atccgccaca
    15001 tcctgctcgg tgcggatacc gccgcccact tgaattttgc agtgggtttc acgaataatt
    15061 ttgccgataa gtgcggtctg tctttgagcc ggatttttcg cccctgtaag atcgaccaaa
    15121 tgtaactgct ccgccccttg tgccaagtag tcggcaaatt gggcaatcgg gtcatcggca
    15181 taagtcgtct gtttcgcata atcgccctga tgtaggcgaa ctacttgccc atcgataagg
    15241 tcgagggcgg ggataatgat ggatttttgc atattattgt tcctgtgtgc ctagcacgag
    15301 ccgtgctagg ctgaataatc ccagtcgcgc tgcgactgcc tctctaagct acggagtagc
    15361 ttggtttatc tagccccata cggctcgtat ggggtacaag cggtcaaatt cttccaaaaa
    15421 cttgcaaaaa accgaccgca cttatttctt cacctgtttc gccacttgtt caatcaccgc
    15481 agcgaaatca atcgcatttt gttgcgtaat caccggtttg ccggtctctg cctccaaact
    15541 ttcccgagcc acttttgcaa catttccgcc ccgtttggca acggttttat tttcttccaa
    15601 gccctgtggg tggctgattt gagcgatgtc tttggtggca gcctcagcaa gcatattcag
    15661 caccaactcg gtggtcgtca tattgtctcg cagactttcc tttttcagcc ctttgaaatc
    15721 cttatattgg cgagtagtca taccgctcca cgcctgtgtg atttcattgg tgagaatcgc
    15781 aaattctctg cccacttgca caccgtgatc ttgccaagta tccgtcagtt cttttcgcac
    15841 ctgaatggct tgcaagcgtt ggttaatcca ctcacgggaa tatccctttt tcaagtaagt
    15901 agccaaagct cggtcaatgg tcaattcagg atcgataatc tcatcaatcc gctctttgcc
    15961 tacctctgcc agccacattt tcaacggctc ggctttcggg cttggcacag actgaataat
    16021 acggaaaatg ccttgtaaat ccgtggcatc ggtttcccgc atttttccgt ctggggcttt
    16081 taatttcaac tgtacgattt tttcgtacag ttcaattccg ccctcttcgt ccttgatttt
    16141 ccgctttaaa tcactccaat aacggcgtgg attgctactt tgcgtcagca cctcgataat
    16201 gtccaccacc gagaaaaacc actcttcttt ttcgctatcc cacaccgagc ggatttggct
    16261 gttttcaaat agtttaattt cgttcatttc tcaccttctt tcactggtta aattaacagt
    16321 aattttgtcg aaataaagcg agaaatcaag ccctattttc aaacttttcg atcgagatca
    16381 caaaatcaga cattctccac aaaattcctg agcaacttcg ccccagcctc gcccgaacgc
    16441 tcggggtgga actgtacgcc gtagaagttt tggctggcaa tggcagcgga aaacggcacg
    16501 ccgtagtcgc aggtggcgat ggtgttctca ttcggtagca caccgtagct atgcacaaaa
    16561 tagaaatgat tgccttgctc aatgtcgtga aatagcggat ggtcggcttg gtattgcacc
    16621 ttgttccagc ccatatgcgg caacggcaaa ccgcattcag gcaagcgttc ggttttgcca
    16681 ctcattaagt tcagtgttgc cacatcgcct tcgtgggaaa agtccgtcat taactgcatt
    16741 cctaagcaga tgccaagcat cggctgggtg gcgttgcgaa tggtgtcgat aagtcggcga
    16801 tcgtgcaaga tcgccatcgc cgccgctgct gttcccacac cggggagtag caatttatcg
    16861 gcagattgga ttttgttgag atctcgcgag atctcagccc gaatgccaag acggtcaaaa
    16921 gcaaatttga ccgaagacag gttggcacag cctgtgtcga tgatggtgag gttggtcatt
    16981 tagtttcctg agttaagtat ttcttatagt gaagtaaaat tgactcaata tatctgtttc
    17041 cttcaaaagc tttcttatcg aatcttttga taattgccaa aaataaacta ttaaagaaaa
    17101 aatcagattt aaaattatta tttag
//
LOCUS       DANLUA010000033        16520 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLUA010000033 DANLUA010000000
VERSION     DANLUA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..895)
                     /locus_tag="PXB44_000805"
     CDS             complement(<1..895)
                     /locus_tag="PXB44_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5281309.1"
                     /translation="MSKIEFEKIPNSIRKPSVLTEYNNKDAVTTLPTNEQEVLIVAPM
                     LNGEAAFTAPQKIFSDVEAENLFGKGSVAHLMVRQAIQNNPLIRLTVVGLKDHEAGIA
                     ATGQVSFTGTVTYAGVVRITIAGTAYEVAAAKGEEAQAIVARLVNVINAASYSPVVAS
                     VESETTLKLTSKAKGEISNEITLATRNTATGLTLEARAFDNGQRNALIAPALASVAGT
                     HYNVIISPFSDDENALALRSHLESVSAPIEDKPAIGVMGWRGTYATGTTLTASLNSER
                     IIVGWYKGATESNAMIAAGLGA"
     gene            complement(895..1074)
                     /locus_tag="PXB44_000806"
     CDS             complement(895..1074)
                     /locus_tag="PXB44_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL5281310.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            complement(1075..1716)
                     /locus_tag="PXB44_000807"
     CDS             complement(1075..1716)
                     /locus_tag="PXB44_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL5281311.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            complement(1713..2138)
                     /locus_tag="PXB44_000808"
     CDS             complement(1713..2138)
                     /locus_tag="PXB44_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5281312.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            complement(2138..2479)
                     /locus_tag="PXB44_000809"
     CDS             complement(2138..2479)
                     /locus_tag="PXB44_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281313.1"
                     /translation="MAKPKKNDNEALNTNENTPLEDKVNEQPEDKTDEQPKPPESETK
                     PDDGALINPIAFEVKLKSIHPQATYGRCGYRFNKETAVEIARKALSDEQIITLANDPY
                     LEFVPVVEGDA"
     gene            complement(2527..3444)
                     /locus_tag="PXB44_000810"
     CDS             complement(2527..3444)
                     /locus_tag="PXB44_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL5281314.1"
                     /translation="MFKKSELLKALDTAFRKEFSAGLKVLEPQWGTVAMKVSSSTATN
                     TYAWLGQFPKMQEWVGDRQIKNMQAQGMTIENKLFESTVAVPRTAIEDDQVGLFTPMV
                     KQAAQSAAELPDDLVFSLLKKGKTTLCYDGQNFFDTDHPVYQNVDGTGTSKTQSNITT
                     GSASGKPAFYVLDDSQAIKPLIWQERTTPEIETKFDPSESDHVFMKDQYLWGVRARGN
                     AGFGFWQLIHRVEDSELNSETLEKVLTAMRTLKGDGDKQLNIRPTTLLVPPSLEFAAR
                     KLLEAELIDGTTNTLKGVLKVVVNPFIAE"
     gene            complement(3444..4514)
                     /locus_tag="PXB44_000811"
     CDS             complement(3444..4514)
                     /locus_tag="PXB44_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL5281315.1"
                     /translation="MFKHKFKLNPIALSFKLNQEANGRIQLFPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSQF
                     AKFLTPEEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYTELA
                     KEKSQVVALSAKVNHPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKAWAEKLGKQDLVALSDFLKTVTPNPALASTQSGGEDPNKGTEKVALSAAEIA
                     AAKSLGLSEAAYIEKYKTEESK"
     gene            complement(4773..5189)
                     /locus_tag="PXB44_000812"
     CDS             complement(4773..5189)
                     /locus_tag="PXB44_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5281316.1"
                     /translation="MIEIQIHGIDEIIATLNRIAAKTANKTDLMRNIAGKMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(5340..6639)
                     /locus_tag="PXB44_000813"
                     /pseudo
     CDS             complement(5340..6639)
                     /locus_tag="PXB44_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(6626..8251)
                     /locus_tag="PXB44_000814"
     CDS             complement(6626..8251)
                     /locus_tag="PXB44_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL5281317.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDTENGDITAQHELFMDIEERDSSIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDLVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKHDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGMPIPVQWAMDKLGIPEAQENEAVLGRSAPPQAVGLSANFPQKMTACECGCGGKV
                     HALSAQTKTGIKEQDALDDLADEAFSVPDFNAQFDPITKKAVAVVMACSSYDEAAEKL
                     AAAYPDLVSEEHDRYLANALFLADLLGAANAES"
     gene            complement(8320..9945)
                     /gene="terL"
                     /locus_tag="PXB44_000815"
     CDS             complement(8320..9945)
                     /gene="terL"
                     /locus_tag="PXB44_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL5281318.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVETPAQRNKLHN
                     WVLKAVLKLGAAGEKFDVIYVGTILHYDSVLSRILNTKGWRRVRFKAILRFPGNMALW
                     DEWENIYLSEEGDDDTMSDLFYQQHKAEMDKGALVSWLARPILYLMKIRASDGHSAFD
                     SEYQNDPVSGDDAIFANSIQYWTELPDDLIYFGALDPSMGKAGASRDPSAILVGGYHR
                     ESGKLYVVEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQR
                     GKPVPATATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDA
                     LEMLWRNALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            complement(9949..10095)
                     /locus_tag="PXB44_000816"
     CDS             complement(9949..10095)
                     /locus_tag="PXB44_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281319.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACIFGAIKLMLNGTDGWGWLLLI
                     AACM"
     gene            complement(10095..10595)
                     /locus_tag="PXB44_000817"
     CDS             complement(10095..10595)
                     /locus_tag="PXB44_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL5281320.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKAAAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTEMPTAAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDELEVQ
                     FKKEFK"
     gene            complement(10603..10857)
                     /locus_tag="PXB44_000818"
     CDS             complement(10603..10857)
                     /locus_tag="PXB44_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281321.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            complement(10857..11114)
                     /locus_tag="PXB44_000819"
     CDS             complement(10857..11114)
                     /locus_tag="PXB44_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281322.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            complement(11107..11262)
                     /locus_tag="PXB44_000820"
     CDS             complement(11107..11262)
                     /locus_tag="PXB44_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281323.1"
                     /translation="MKLIKKLRQRWQQWRFYRRRPDLAELHNVVRAAVAQGNQHPVGR
                     AKRGRYD"
     gene            complement(11277..11636)
                     /locus_tag="PXB44_000821"
     CDS             complement(11277..11636)
                     /locus_tag="PXB44_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5281324.1"
                     /translation="MITQQIAGVVGVVSTILAYAFYKSWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAKIQQKHVSETRRIQPSAIDEQLHQHGYFRDDNGLHGVRSDLPEP
                     CGHNGNETPSACTQSDL"
     gene            complement(11633..11878)
                     /locus_tag="PXB44_000822"
     CDS             complement(11633..11878)
                     /locus_tag="PXB44_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5281325.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFMSWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKQKERSREAEE"
     gene            complement(11881..12414)
                     /locus_tag="PXB44_000823"
     CDS             complement(11881..12414)
                     /locus_tag="PXB44_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5281326.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            complement(12500..12925)
                     /locus_tag="PXB44_000824"
     CDS             complement(12500..12925)
                     /locus_tag="PXB44_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL5281327.1"
                     /translation="MSEFESVEHYLPETVKEIVGVIGLPATEKLIKAFGGFSFQFSNG
                     KLYFNKLKEVLGQDDAVKLQAYMGACEVYLPRCETALRMLRNQQIYADYCQLTEQGGL
                     SGRLAIMQICPKYSVCDRVAWEAVRYYQRKHTVSQATLF"
     gene            complement(12996..13211)
                     /locus_tag="PXB44_000825"
     CDS             complement(12996..13211)
                     /locus_tag="PXB44_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5281328.1"
                     /translation="MIENPEFGYTPSNLKMLRKHHNLTQTEVANILGVGQRIVMRWET
                     ELSSTAARSDMPYTKWAELLKNLQKNF"
     gene            complement(13230..13622)
                     /locus_tag="PXB44_000826"
     CDS             complement(13230..13622)
                     /locus_tag="PXB44_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281329.1"
                     /translation="MTDYQKLLNALLNDADEDCFAVYGLRATTDVHQVGDELGNSFVW
                     VDGEKTDEELDGICTMGIQNADEAGLLNAIKNLGRDACKKFDVEFKGFQSYCGQNFIL
                     VKGDSARGGEDKGESIIRNPIVVAVFNS"
     gene            complement(13748..14095)
                     /locus_tag="PXB44_000827"
     CDS             complement(13748..14095)
                     /locus_tag="PXB44_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281330.1"
                     /translation="MKELLLTTLCLLSLPAIAMTEKAEQETANALVSGDYQQLRNVAY
                     GMETGSFGHDHNPIAACALRRVILLVNSDKVDMTDFNNEAIACRKIEVTDNQQAWETA
                     FTIAKSISATKKK"
     gene            complement(14184..14609)
                     /locus_tag="PXB44_000828"
     CDS             complement(14184..14609)
                     /locus_tag="PXB44_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL5281331.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDDNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDDQLHKLVQILASYNHRHGMSVK"
     gene            complement(14593..15147)
                     /locus_tag="PXB44_000829"
     CDS             complement(14593..15147)
                     /locus_tag="PXB44_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281332.1"
                     /translation="MKLCKCPICHSDISFEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYPPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            complement(15147..15317)
                     /locus_tag="PXB44_000830"
     CDS             complement(15147..15317)
                     /locus_tag="PXB44_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281333.1"
                     /translation="MEYQKIPEYRLCKKKSGEIVLQQLVICELTPATWHNPYPDSRTY
                     WVDLETIEEGTE"
     gene            complement(15388..15963)
                     /locus_tag="PXB44_000831"
     CDS             complement(15388..15963)
                     /locus_tag="PXB44_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL5281334.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLDVKGFKVGKFNDEFYLVKPDKRYEKGKELAE
                     DFKAINEIYEKHRDFSPYILKRLNMLYFVSDFSYSPTSGRSYLAVAGICENTLLVEMP
                     MPIDKNDEPFPEIAEGLVEIKESEFLAIQGK"
     gene            complement(15986..16168)
                     /locus_tag="PXB44_000832"
     CDS             complement(15986..16168)
                     /locus_tag="PXB44_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281335.1"
                     /translation="MTEQEKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            complement(16235..16381)
                     /locus_tag="PXB44_000833"
     CDS             complement(16235..16381)
                     /locus_tag="PXB44_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5281336.1"
                     /translation="MKTEQKATEFDRYKHYAEKAAEAERKGNYAEAQDHWKVAKLSAK
                     KTAN"
BASE COUNT         4346 a         4073 c         3086 g         5015 t
ORIGIN      
        1 ccgcaccaag ccctgccgca atcattgcat ttgattcggt tgctccctta taccagccta
       61 caataattcg ctcactgtta aggcttgcgg ttaaggttgt gccggtggca taagtgccac
      121 gccagcccat tacaccaata gccggtttat cttcaatcgg ggcagaaacc gattctaaat
      181 ggctacgcaa ggcaagggca ttttcatcat ctgaaaacgg cgaaataatc acgttataat
      241 gcgtaccggc aacactcgcc aacgccggag caattagggc attacgctga ccgttatcaa
      301 acgcacgagc ctcaagggtt aaacctgttg ctgtattgcg tgttgcaagc gtgatttcat
      361 tgctgatttc gccttttgct tttgaggtca gtttaagtgt cgtttcactt tccacacttg
      421 ccaccacagg gctataactc gccgcattaa tcacattaac taaacgagcc acaatcgcct
      481 gtgcttcttc gccttttgcg gctgcaactt cgtaagctgt gccggcaatc gtaatacgca
      541 ccacaccggc ataagttact gtgccggtaa aactaacttg ccctgttgct gcaatacctg
      601 cctcgtggtc ttttaagccc accaccgtta aacggattag cggattattt tggattgctt
      661 ggcgaaccat taaatgagct accgagcctt taccaaataa attttccgcc tccacatcgc
      721 taaaaatctt ttgcggagca gtaaaagcag cctcaccgtt tagcattggt gcaacaatta
      781 gcacctcttg ctcattggtc ggcaaggtgg ttactgcatc tttgttgttg tattcggtca
      841 gcacacttgg tttgcgaata ctgttcggga ttttctcaaa ttcaattttt gacattattt
      901 gccccctgtt tctttagcgg atttggtttc tttgacgaca atcaaatcgc catccgcaat
      961 gcggcgttga tagtaaaggg tgttttcaac ctctactgcc tcttgttcaa tatagtgatg
     1021 cggtcggttt tctaccggca cacgcacccc tttagcggct ttcactttta acatctaatc
     1081 accatcctta aattctgttc taaatgccgt ttcggcattg ttattcgggt caaaaatccg
     1141 attattaaag cccgataaat cgccttgcgg ttcatctagc ttgccttgat actgcttaaa
     1201 gacttgctca ttcggctcaa ccccttcggg gaatctacca tcatcaagca cattgcactc
     1261 gttgtagctg atttcaaact caatagcata agcagagagt ttctctttct tcacttccgc
     1321 attattccaa atcgtgcgga ttcgcttagg ctggataggg tggactaaac cgtctaaagt
     1381 ctggttaatc aataaggttt tcaccgctcc cagcaactga ttaaccccca cctcacgact
     1441 tgttacccca cctaaacgcc cagcagtctg atttcgcatt gagcgagtaa taaccaacac
     1501 cacaaacaca tcagtggctt gatagcgttt acctcttact cccattgatt tcgcctcgaa
     1561 cgccgagcca ccataagaga caagacaagc aggcaaacgg agaacatcta acttctcatc
     1621 gttaatttcc ccggcatacc cagcaacggc atacaccatt ttgcctaagc ccagcgttaa
     1681 acgctcaatc aaagcacgct caattttgct aatcatcgct tatcacgccc ccaaatacgg
     1741 ttgcccccgt taaaaaactg cactgtattt tcgccagtag ccgattcttc ctcattggtt
     1801 tcatcaaccg ctaacgacac tacgccctta gagatgtttt ccagctcttt taagcaaagt
     1861 ttgtagcggt tttccacctc ttcggtcatc gttactaatg atttgctggt taaatgataa
     1921 cgagccaaat cacagcaaat acgctttaaa ttttgcggta cagtctttaa tggcagacgg
     1981 taacgcccac ttaaatagcc atcaatttgg ctggaagagt ccgctaaagc tacttcaaga
     2041 gtcgcttcat caaccacgcc taccatttca cggtcggtta gctcaatgga ttcctgttcg
     2101 ccaattcgca aaacgaaatc atcaaccaac gcatacatta cgcatcgccc tcaacaacag
     2161 gtacaaactc caaataaggg tcgttggcta aggtaataat ttgttcatca ctcaaggctt
     2221 tacgagcaat ttctaccgcc gtttctttat taaaacggta gccacaacgc ccataggtgg
     2281 cttgtgggtg gatagatttg agcttaactt caaaagcaat aggatttatc aaagcaccgt
     2341 catcaggctt agtttcactt tcaggcggtt taggttgttc atccgtttta tcttcaggct
     2401 gttcattcac cttatcttca agcggtgtgt tttcgtttgt gttcaaagct tcattgtcat
     2461 ttttctttgg ttttgccatt ttgctaccct ctcaaaatgg gcgtattgct acgcccaaca
     2521 ttaaacttat tcagcaataa atggattcac taccactttt aacacacctt ttaaggtgtt
     2581 ggttgtgccg tcaattaatt ctgcttcgag taatttgcgt gcagcaaact caagcgaagg
     2641 tggcacaagc aaagtggtcg ggcgaatatt taactgctta tcgccatcgc cttttaaggt
     2701 gcgcattgca gttaatactt tttcaagggt ttcactattt aactcactat cttcaacacg
     2761 gtgaattaac tgccagaaac caaaaccggc attaccacga gcacgcacac cccataaata
     2821 ttggtctttc ataaagacgt ggtctgactc acttggatca aacttcgttt caatttcagg
     2881 tgtagtacgt tcttgccaaa ttaacggctt aatggcttgg ctatcatcta gcacatagaa
     2941 cgcaggctta ccgctggcag agcctgtggt gatattactt tgtgttttac ttgtgccagt
     3001 tccatcaaca ttctgataaa cagggtgatc ggtatcaaag aagttctgcc cgtcatagca
     3061 aagggttgtc ttaccttttt tcaacaaact aaataccaaa tcatcaggta attcagccgc
     3121 actttgtgcc gcttgtttca ccattggggt aaataagccc acttggtcat cttcaatggc
     3181 agtacgaggc acagcaacgg ttgattcaaa taatttattc tcaatcgtca ttccttgtgc
     3241 ttgcatattt ttaatttgac ggtcgcccac ccactcttgc attttcggga attgtcccag
     3301 ccaagcatag gtattagtag cggtgctgga gcttaccttc attgcaaccg ttccccattg
     3361 tggttcaagc acttttaaac cagcactaaa ttctttacga aatgcagtat caagtgcttt
     3421 taataattca gattttttaa acattacttg gattcctctg ttttatattt ttcaatgtaa
     3481 gctgcttcgc ttaaaccaag cgattttgct gctgcaattt ccgcagcaga taacgccact
     3541 ttctccgtac ctttgttcgg gtcttcaccc cccgactgcg tacttgctaa agcggggtta
     3601 ggtgttaccg ttttcaaaaa gtcggataaa gccactaaat cttgcttacc gagcttttcc
     3661 gcccacgctt tttgagccgg caataaccga ccgtcagaaa gagcggtttg aattaacgca
     3721 tcacgctctt tatcgttgat ttgctgtttt aagccgttca gctcggtttg cactgcttgc
     3781 aagtcgctta acgccacaaa cttcgccggg tcagggtgat tcactttggc agagagtgca
     3841 accacttggc ttttctcttt tgccagctcg gtataaacat cacttaacgc caccgggctg
     3901 tcgcctttgg ctttctcaag tgcggtcagt ttttcggtaa tctgctcatc ggtcgcattt
     3961 gccgacaatg caaataattt aattaagagt tctttcattt ttggattttc ctcttcgggg
     4021 gttaaaaatt tggcaaattg gctagacatc gcaaccacct cttgcagatt atgcaaggca
     4081 gggcggttag ttaaggcggc attaagtacc ttaattacca ttccgctgcc gtctgtcaaa
     4141 aacagtggcg agagatagcg ataaacgccg tctttaatct cttgggtcgc ttgtggtgtc
     4201 cacttaacat ccgcaaacaa gccttcgccc gagatatatt ccgccttggt aatccaaccc
     4261 gcagcagggt tgcctttgcc gttctcggcg atgtacaggg tctgatgctc ataatcaatc
     4321 atcagatcaa tcgcagtgtt gttaatatca tcggctaacg catagccatt ctcgtcacct
     4381 acataccaac gtcctccacg cccgtcttga gcctcaaacc aaccaaaagg gaaaagttga
     4441 atacgtccat ttgcctcttg gttgagttta aaactcaatg caatggggtt tagtttgaat
     4501 ttatgcttaa acacaaaaac acctcaaaac aaattaatga ggctaaagaa tagaagattg
     4561 agaaaaaaga gtaacgggga gcaacttcca caagttgcaa attttcagaa aaaaacaacc
     4621 gcttgtattc agcgaggaat gtaacccatt ttaaaacgct ttaaatccgt tttaaaaaat
     4681 tttaaaaaag ttggaatgat aaatcaccat aaaaaaaaca aaaacgccac agagagcgat
     4741 ttaggggcta tctgtggcga ttatcttatc gcttaatcca gcagtttttg ccagtaggct
     4801 tgcacatcgt ccaaaatatc ttgcctgtct tgcgaggtta attgtaaaaa aggtcgggct
     4861 tcaattttta ctttacgccc acgcccagcc ataccaccga attgatgaat agcagcataa
     4921 ggctcatttg tgccaacaat cgccgaatca ttattataat cagcagtaat gctgcccatt
     4981 aaattttccg tatcaactaa cggcgtgcct tgccgatgtt ttatacccag ccatttcggg
     5041 cgaccgccct catcaaaatt ggtcagtacc gccgattcca ttttgccggc aatattccgc
     5101 atcaagtctg ttttatttgc cgttttagca gcaatacggt taagcgtggc aataatttca
     5161 tcaatgccgt ggatttggat ttcaatcatt tttaaccttg caagttgatt tttaaaataa
     5221 acaggggtat attcctttta cggttattag aaaagcggtg ataatctccc tgccgcagcc
     5281 tatttgagat ggcaagtcct gctcaaatga ccgagagcgt gtgtaagtga ccgggagcct
     5341 tacctaataa ccgtattttt caacagcagc ttatccaatt ctttttgtcc aatctccctt
     5401 gttgatacca gaaataattc accaaactct tctaagtatt tcaccacaac aagaatacga
     5461 taatgctcaa agtttcgtat aaagctgaaa tttttgcctt cttgcaaaat atgattaggc
     5521 tgaaaaatta catcaggaat atttgcatac acatcaaacc caaaatcctg cccctcacgg
     5581 ctataaaact gcttaatcag tgtgtcatcc gaaagccata ccgtgccgac tttactgtcg
     5641 attaagcgtt gtgtttcctc acttaaccga ccagcagcaa atttaaagtt ttgattaagg
     5701 gcatttcgca ctttaaccat ctcatcaggc gagagttttt tcgccccatc tttggcaagt
     5761 gccacagcct cttctaactt gttaaacgcc tgcttaaatt caccaccgct catttctgct
     5821 ttggtaaatt ggtaagccag cttttcaggg tataaatcca aattcggctt ataggttaaa
     5881 cgccccacat tataatcaaa acctttatca gccaatcgca ccgtaccatc agccaattta
     5941 aagccaatgg tttcctcttg gttgcctaac ttatctttcg gtcgttttgc cttcactaaa
     6001 aacggctcgc tactgccgac ttcgtcaatc cctttacgct ttaaatcccg ctcacttaac
     6061 gcaatcacgg tacaacggca attaaagccg ttcggcggat aaaaagtcgc ccaaaacgga
     6121 tcgtcataac gataaatctt accctgtaat gcttggtgag cagggcgggt gcgttggtcg
     6181 cctaccgccg aatattgcca ataagggcga ttatccacat tatccatata ccgctgataa
     6241 cgagccgcag aataagcctg ttgcatattg gtacgataaa tcgtattcaa gcggcgaggc
     6301 gtgccgaaat gctcgccggt tttcgggtcg gcaagcagtt tgccatcaat gccacgacta
     6361 atcgtcgtgt ccatacaccc agcctttccg ttcaaattct gccgtaagcg ttttcttcca
     6421 ttcattaaag gatttcccct cacgccgtgc tttttccatc gactcataaa tatcacgggt
     6481 catttcaagg ctagtgagct tgctaatggt ggcggctcgc cccaatgcag aatcaaataa
     6541 ttcctgctta aacactttat tcgcaagcaa cttcttctgc cgtaaaaact caatcgcctg
     6601 cttaggctct agtccaatgg caaaattaac tttctgcatt cgcagcccct aataaatccg
     6661 ccaaaaataa cgcattcgct aaatagcggt cgtgttcttc actcactaaa tcagggtagg
     6721 ctgccgccaa tttttctgct gcctcatcat aagagctaca cgccattacc accgccaccg
     6781 cctttttcgt gatggggtca aattgggcgt taaaatccgg cacggaaaaa gcctcgtctg
     6841 ccaaatcatc taaggcatct tgctctttaa tgcccgtttt ggtttgggcc gacaaagcgt
     6901 gaaccttgcc cccacaaccg cattcgcaag cggtcatttt ttgcggaaaa tttgcggata
     6961 accccaccgc ttgtggcggg gcagaacgcc ctaacaccgc ttcattttct tgagcttcgg
     7021 gaataccgag tttatccatc gcccactgca ccggaatcgg cattccaata tccaccaatt
     7081 tcggcaagct ctcggcaaat aacgccaagt cggcaggctc tttggtatca aactcaaaac
     7141 tcggaatgcg ggttgggtcg atattgccaa aattaatttg cagaaacggc agaataattt
     7201 gttgggtaaa cgtctgcccc agctgtttca cgtccgacac caacaaatca cgccgcactt
     7261 cgttatgcac attgccgagt gcattggtag agcttttgcc gtctgcaccg ctggttaagg
     7321 tttgccccaa aatcaagcgg gcgatagact tctcacacca atccaccatt tgcaaaaacg
     7381 gattattgcc ggctgcaccg cctgcattag cggcgttatg caactcaata ctcatcgact
     7441 ccggcataat tccggcagcg ttgtgtccga tttccgctaa tgcacgttta agcgtttgtt
     7501 tttcgtttgg tgttgcccct gcaccgtatt taccaatgcg gataggcata ccatacagct
     7561 ccaaaaactc ggcaaagtcg tggatagagt agtgtttaaa catatacagc caagccagcg
     7621 agcggaataa attattgcgt gccgcctgtg tggagcggga tttatgcgta tgcaccaccc
     7681 agccaaattc tcgtaatggc tcaccttcgg tattttgtcg ggttttcagc aacagattgt
     7741 cgtgcttatc ccacttaaac cacgattgag gacggtggat aaaggcgttc ggataccaca
     7801 atttaccgtt aaacgcccat tcaatttcga gggcagcaaa accgtgtccc acagcgtcca
     7861 tacagtccat caccaaatct tcaaggttgc cgagctgata aaataactca tcaacctcat
     7921 cacgcagttt ttcttcctgc ggtgtggcat tgcgaggggc aacaatcgcc caatctacgc
     7981 ctaatgcggc acgcttacgg gtttggatat tggcaaaaat agagctgtcc cgctcctcaa
     8041 tatccataaa taattcgtgt tgagcggtaa tatcgccatt ctccgtatct tcaaaaatcg
     8101 ctttcatttt agcaggcgta ataaaattgc tcggatggtc ggagagtacc cgacctgtag
     8161 cggtaatttc ggctaaatcc gtctgcaatt cttgattttt aatcgggttt aaattcggtt
     8221 taatctgctt gttatgcttc tttttgctca taaattttcc ttttcagagc ttagttacgc
     8281 tcaatatagt ccccctcttt agcaaagagg ggagttacat taatgccgcc acttactgcg
     8341 atactcaccg tcaaaatcat ctacactctc ccactcaatc ggggcagagc tgctgagtgc
     8401 atttcgccat aacatttcca acgcatccgg accatcatcg tgatcggctt tcggaaaatg
     8461 gcgaagctgg ctttcaagcg tagattgcga gcggtgcaac aaaatcaaac cgttggcaat
     8521 atgcggttgc aagctctcaa tccgtaacat cttgtcactg ttcggcttgg ttgccgttgc
     8581 cggcaccggc ttaccacgct gggcagaacg cttgaccagt tccgtctgca aaaactcctg
     8641 aaattgcacc gtctccacaa accagcggtg acagttatat tgcgaatgca gtctaatcac
     8701 gtcctcaata attaaatccg gcaatcgttt tttgatttgt gcctcaacaa catagagctt
     8761 gccactctca cgatgatagc caccgaccaa aattgcagat gggtcacggc ttgccccagc
     8821 tttccccatt gaggggtcta acgccccgaa ataaatcagg tcatcaggca gttccgtcca
     8881 atactggata ctgttggcaa aaatcgcatc atcacccgag acagggtcat tttgatactc
     8941 ggagtcaaat gctgagtgcc catcactagc acggattttc atcaagtaga ggatagggcg
     9001 agctaaccaa gagactaacg ctcctttatc catttctgct ttatgctgtt gataaaagag
     9061 atcagacata gtgtcatcgt ccccttcttc cgagaggtat atattttccc attcgtccca
     9121 taacgccata ttgccgggaa agcgtaaaat ggctttaaaa cgaactcgcc gccaaccttt
     9181 tgtatttaaa atacggctaa gcacactgtc ataatgtaga attgtgccga catagataac
     9241 atcaaacttt tcacctgcag caccaagttt taaaactgct tttaaaaccc agttatgcaa
     9301 tttattacgc tgtgcaggtg tttctacgct ttcatcattc tcaatatcat caagtactac
     9361 taaatccgga cgataagctc cgtggcgacg accacgcaat ttttgcccag cccccacagc
     9421 ttcaactttt tgttttttag ccgtcataat gacacctgca cgccaaacct tgccttgtgc
     9481 aacctcgggg aaatcaattc ttaaacgagg gttagattca atttccactt taatagcttc
     9541 aagcatactg tatgcctgtt ctttggtgtc catcgcaata atgatgtatc tttttaaatc
     9601 acggactaaa caccacagcg ggaataattg cgtacaaatc gttgattttg cctcgccacg
     9661 aggggctgca atcgcttggc ggactgactt agaaagatca cttaccgaaa gcggtaaatt
     9721 ctcaaagaga taatgatgca actgtgattt atgttcagac cgcacatagt gtggaaaata
     9781 agcctgcaca aaatactcaa agccgtgaac agggtctaaa accttttgac ggcgttcgct
     9841 aatggcttgt ggtttatcgt cccaaccctc aaaactcgct tcgatatttt gttgtaactg
     9901 tcgccgtaat tcttccagtt ctttttcaaa ttctttgatt ttcataaatt acatacaagc
     9961 tgcgataagc aacaaccagc cccaaccgtc tgtaccgttg agcattagtt ttattgctcc
    10021 gaaaatacaa gcaagttgga caatccaacg gaaagagtaa tgcttatgca ctacagtttg
    10081 ttttttctta gccattactt aaactccttt ttgaattgca cttctagctc atctaacatt
    10141 tctaaaaagt caggcaataa atgcggtttc ttggttttga cgatattcgc catcatctca
    10201 atcgcacgca tagcggttgc cattgcactt gtttcaggca atagtttctt gctcgatgcc
    10261 gtcatcttcg caaacgaatc agccagagca gctaatgcct caaccttagc tgcggttggc
    10321 atttcggtgt tttcctcaag ctccgtcatc aacgtgcggt acttaattaa aaaaccggct
    10381 aacaagcctt gagcgatatt ctcaatacca ccgcttgcca tcgcttgcac atcacgagct
    10441 ttttcccagt tatcaccgtt cttttcagca gccgctttcc aacgccgtgc cgtgccgaat
    10501 gatacccctg cacgttctgc tgccatttcg aggcttaaac ggtcaaacac ataggcttgc
    10561 cgaaccgctt tttgtacttc aacatcgtgt gccatacgcc ctctacattc caaatttcag
    10621 gcgtaacaac tcaaaaccca cagcaacaat gccaccaccc aagccaccgg caactacagc
    10681 atcacggcgg tttttacgtg ccatttcatt cactaaacga ttagtcgctt ggatttcacg
    10741 gtgtaagcgt tcgatttctt cgttttgttt atccacctta tcgtttagct catttaatcc
    10801 acctaaaatc aggtctaatt tttccgaatc ggtttgcttt tgttttttac ggctcattac
    10861 ttatccgcct ttttatcaag tttttggttt acttctttga gcgagtctaa aatatcgtcc
    10921 agcttttcct taatccctct attcacttcg tgggcaagct ctttggattg atatttgctc
    10981 tccatttctg cttttagctc ttttaccgtt tgctcattgc ggttgatacg atcaaatatg
    11041 accttgccta caaacgccac taacggagca actacaaaag agatcagcat ctgaaacagt
    11101 ttttcatcaa tcataacgcc cccttttggc tcgcccgacc gggtgttgat taccttgtgc
    11161 caccgccgca cgcaccacgt tatgcaattc tgcaaggtca ggacggcggc gataaaaacg
    11221 ccattgttgc caacgctgtc tgagtttttt aattaacttc attctggcaa acttcctcat
    11281 aggtcagatt gtgtgcaagc acttggcgtt tcgtttccat tgtgtccgca cggctcgggt
    11341 aaatcagacc gaacgccgtg caacccgttg tcgtcacgga agtaaccgtg ttggtgcaac
    11401 tgctcatcaa tggcactagg ctgaatacgg cgagtttcac taacgtgttt ttgctgaatt
    11461 ttggcatttt taacctctgc atttttttgt tgaatttcga ccgcttgttg ctgcttttcc
    11521 gcctttaatg tagcggtaag tttccgctcc tgctttaatt gccagctttt ataaaaggca
    11581 taggctaaaa ttgtgcttac tacaccaacc acacctgcaa tttgttgagt aatcattctt
    11641 cagcctccct tgagcgttct ttttgtttta acgcacttac cacccctttt gtggctgggc
    11701 tacccaatac acaaatacca gcataagcaa accaccagtc ggaaataaac ggcttatcgt
    11761 aaaggtaggc gtgaattaaa atgcccgata acactaacca gctcataaac tgaataaagc
    11821 cagtggtgct ggctgtgccg tctttattgg taattaaatc aagtaaaaat tgcttcacga
    11881 ttattcctcc accgtgtctt tggcaaaata acgcaaatta cccgccattc ggttaatcca
    11941 acctctacca aaacggtcaa agtgtttgag tttggtgtaa aactcaaggc gttctgcctg
    12001 aaataacaag cccacatccg caaccgaata acgctcaatc gcagccaaag tcactttacc
    12061 gataatgccg tcatcagcca cacctaccgc tcgctgtaac attcggctag cattgcccga
    12121 accgtggttt acacacgcat ctaaaaactg aaaagccaac tcaggcggga actgctcaca
    12181 gttataacgt tgccaaaagg ctttgaggta aatagttttc gcatccgcac ggctcattgc
    12241 acgcatagag ccggtatagc cattcgcacg ggcagtatgg atagtgatac cccaattcgt
    12301 ttcgccaccg gggtctttcg ggtcgttcac ataaccgccc tcgtgtccga taagacggtc
    12361 aaatgcaaga ttaaagtcag caatagttgc cttgtttttg ataggttcag tcataaaaaa
    12421 agcctcttta aattcaatga tgatttaaag agactttaat gatttagagc aacggggagc
    12481 aggcgaaagg attccacact taaaacaagg tagcttgcga aacggtatgc ttacgctgat
    12541 aatatcgtac tgcctcccac gcaacacgat cacacacgct gtatttaggg caaatttgca
    12601 taatcgcaag cctgccactt agccctcctt gctcggttaa ttgacaataa tcggcataaa
    12661 tctgctgatt acgcaacatt cttaatgcgg tttcacaacg tggcaaatag acctcgcaag
    12721 ctcccatata agcctgtaat ttcactgcat catcttgccc taatacctct ttgagcttat
    12781 taaaatagag ttttccgtta gaaaattgga atgaaaagcc accaaaggct ttgattagct
    12841 tttcagttgc cggcaaacca atcacgccca caatttcttt caccgtttcc ggcaaataat
    12901 gttcgacact ttcaaattca gacataaaaa atccccttga ataatttcca cgaaagccat
    12961 tatccaaggg ggttattcaa atctgtaata cttacttaaa aatttttttg caaatttttt
    13021 aaaagctcag cccattttgt gtacggcata tctgatctag ctgcggttga tgacagttct
    13081 gtctcccacc gcattactat tctttgcccg acccctaata tgttagctac ctctgtttgt
    13141 gtgaggttat ggtgctttct tagcattttt aagttgcttg gtgtgtatcc aaactctggg
    13201 ttttcaatca ttcacctccc tccattaaac tagctattaa atacagcaac aacgataggg
    13261 tttctaataa tgctctcgcc tttatcttct ccgcctcttg cactatcgcc tttaactagg
    13321 ataaagtttt gcccacagta tgactggaaa cctttaaatt caacatcaaa ttttttgcaa
    13381 gcatcacgac ctagattttt aatagcattt aacaaacctg cttcatctgc attttggatt
    13441 cccattgtac aaatgccgtc taattcttca tctgtttttt caccatcgac ccaaacaaaa
    13501 gaatttccta attcatcgcc aacttgatgc acatctgtag ttgctcttaa accataaaca
    13561 gcgaagcagt cttcatcggc atcgtttagt aaagcattta aaagtttttg gtaatctgtc
    13621 attttaattt ctcctgtttg gctatgggtg cattatagta atataattac ctattgcaag
    13681 tgatttttcg tttttttctt aaattttttt atgtaaataa aaaagaccgc ttgaaagcgg
    13741 tctaaattta ttttttcttg gttgcagaaa tggattttgc aatggtaaag gcagtttccc
    13801 aagcctgttg attatctgta acctcaattt tacggcaagc aatcgcctcg ttgttaaagt
    13861 cagtcatatc aaccttgtcc gaattaacaa gcaaaataac acggcgtaaa gcacaagcag
    13921 caataggatt gtgatcgtgc ccaaacgaac cagtttccat tccgtaagct acatttctta
    13981 actgttgata atcaccactt acaagagcat ttgctgtttc ttgttcagct ttttctgtca
    14041 ttgctattgc tggtaacgac aataagcata aggttgttaa tagcagttct ttcattttaa
    14101 atacctttaa aagttgatta aaaaacacct aaaatctacc acaaaaaagc ccactattta
    14161 aagtgggcta tgcaataaat ttattattta acactcattc cgtgcctatg gttataactc
    14221 gccaatatct gcactaattt atgcaactga tcatctgtca gccattgcac ccgctcaata
    14281 ccaaacgagc gtttggcaat ggcgtgggcg taatcccacg gcttttggct ttgatacagc
    14341 aacgccccga ttttatttat caacccttgc cggtgagggg cggttttact tgctgttggg
    14401 cgtttaccgt attttttcga cttcaccaca aaccctttag ctttcattgt ttgcagtacg
    14461 gtcattaatt cgctatcggt catcattgag cagcttggtt tatccaccgc ctctaacaac
    14521 agcattttat aggcgttatc atccagtttt agctcatttt tgccgatatg gattttctgt
    14581 atcatctgct ttctagtttg cggttgcatt ttgcttttcc tcctgccatt tcagccaaat
    14641 ttgataactt tcggtgttct taacggcttc cagttgcccc atttgcttca ttcgttccac
    14701 atacatcacg gcatcgtgtt tcttttggtc tgcttgccgt tgctgttgct caaggctact
    14761 cactgctttc gcttcacttc ttactactgc aaattgtggc ttcacgcttt catatacttt
    14821 cttcaagtaa ttatgatttg ccagcggctc acatctgcca gtttcacgcc gattccgtct
    14881 tacttgttcc accgtatcca ttaaggcttt ttctaacaca ttagagggag ggtaaagctc
    14941 caataaactc tgcacaattt tcaaggctcg gctattggca aggctgtttt tctgtggctt
    15001 aaataaccct aaatacgcca ccaccgcacg cccacaattc ccaccgatat tgactatcgt
    15061 atttaacaac tcacgccctg catcatcttc aatcaacgcc tcaaacgaga tgtcggaatg
    15121 gcaaatcggg catttgcata gtttcatcat tctgttcctt cttctattgt ttcgagatca
    15181 acccaataag ttcgactatc agggtatggg ttatgccaag ttgctggagt tagctcgcaa
    15241 ataactaatt gttgtaacac aatctctccc gatttctttt tacataaccg atattcagga
    15301 attttttgat attccatttt atgctcctat aaaacacatt attcagccca cttaaatagg
    15361 ggttaaatgg gctgtaaatg ggttttatta cttcccctgt attgctaaaa actcactttc
    15421 tttaatctca accaatccct ctgcaatctc aggaaatggt tcatcatttt tatcaatagg
    15481 cattggcatt tcaaccagta aggtgttttc acaaatgcct gctacggcta aataacttct
    15541 acctgatgtt ggggaataac taaaatcact tacaaaataa agcatattca aacgttttaa
    15601 gatatagggc gaaaaatcac gatgtttctc ataaatttca ttaattgctt taaaatcttc
    15661 tgcaagttct ttcccttttt cgtaacgttt atcaggtttg acgaggtaaa actcatcatt
    15721 aaatttaccg accttgaacc ctttaacatc taaaacttgg ctaatattgg ttgctttaca
    15781 agcaatccct ataactattc tatgaaacca gcctgaccta ctaattaatc catcattgaa
    15841 aggatatttt tctaaaatag cattgagctt atccttgcgt tcttctttag atacttgata
    15901 atctgcataa atgcttttta gtggctcttg ggttatatcc cctttaaaaa atctaaattt
    15961 cattcttaac tccttactgg ggtggttaat gtttccgatt tgccgttaat aacttcaaat
    16021 gcacctttgg caacaaatcc tgcaccgtgc cgagataaac accactgcga ataaagtcac
    16081 tctgttttaa atatttttgt gcctgaataa tctgcattaa cgcctcgttt aattggctgt
    16141 taagttcgtt tttctcttgt tcggtcatca tctcctccta aaacggtttc ttatgcatac
    16201 gtttacaaaa taccgctcgt tgccccgccc agtctcagtt agcggtcttt tttgctgata
    16261 attttgcaac tttccaatgg tcttgggctt ctgcataatt gcctttacgt tcggcttccg
    16321 ctgctttctc ggcataatgt ttgtagcggt caaactcggt cgccttttgt tctgttttca
    16381 ttttttcctc ctgtggttaa aacactttat aaatgccctt ctatcttgtc cccctctttg
    16441 aaaaagaggg gctaggggag atttttaaag ggcattgaaa aggtttttta actaagactt
    16501 tctttttaac tgatttaaaa
//
LOCUS       DANLUA010000034        16315 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLUA010000034 DANLUA010000000
VERSION     DANLUA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16315
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            8..2083
                     /locus_tag="PXB44_000834"
                     /pseudo
     CDS             8..2083
                     /locus_tag="PXB44_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2127..3563
                     /locus_tag="PXB44_000835"
     CDS             2127..3563
                     /locus_tag="PXB44_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL5281337.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
     gene            complement(3705..3884)
                     /locus_tag="PXB44_000836"
                     /pseudo
     CDS             complement(3705..3884)
                     /locus_tag="PXB44_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011625997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3968..4507)
                     /locus_tag="PXB44_000837"
     CDS             complement(3968..4507)
                     /locus_tag="PXB44_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5281338.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEYKSVEDQT"
     gene            4606..4857
                     /locus_tag="PXB44_000838"
     CDS             4606..4857
                     /locus_tag="PXB44_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5281339.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(5053..5253)
                     /locus_tag="PXB44_000839"
     CDS             complement(5053..5253)
                     /locus_tag="PXB44_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5281340.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(5312..5824)
                     /locus_tag="PXB44_000840"
     CDS             complement(5312..5824)
                     /locus_tag="PXB44_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL5281341.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(5837..6991)
                     /gene="tgt"
                     /locus_tag="PXB44_000841"
     CDS             complement(5837..6991)
                     /gene="tgt"
                     /locus_tag="PXB44_000841"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL5281342.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(7050..7190)
                     /locus_tag="PXB44_000842"
     CDS             complement(7050..7190)
                     /locus_tag="PXB44_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281343.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            7284..7856
                     /locus_tag="PXB44_000843"
     CDS             7284..7856
                     /locus_tag="PXB44_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL5281344.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            7958..8176
                     /gene="cspD"
                     /locus_tag="PXB44_000844"
     CDS             7958..8176
                     /gene="cspD"
                     /locus_tag="PXB44_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL5281345.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            8266..9180
                     /locus_tag="PXB44_000845"
     CDS             8266..9180
                     /locus_tag="PXB44_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5281346.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            9299..10159
                     /gene="araC"
                     /locus_tag="PXB44_000846"
     CDS             9299..10159
                     /gene="araC"
                     /locus_tag="PXB44_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL5281347.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            10346..11320
                     /locus_tag="PXB44_000847"
     CDS             10346..11320
                     /locus_tag="PXB44_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5281348.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            11375..12865
                     /locus_tag="PXB44_000848"
     CDS             11375..12865
                     /locus_tag="PXB44_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5281349.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            12849..13808
                     /locus_tag="PXB44_000849"
     CDS             12849..13808
                     /locus_tag="PXB44_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281350.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            13811..14761
                     /locus_tag="PXB44_000850"
     CDS             13811..14761
                     /locus_tag="PXB44_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281351.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            14774..>16315
                     /locus_tag="PXB44_000851"
     CDS             14774..>16315
                     /locus_tag="PXB44_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL5281352.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNPLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQR"
BASE COUNT         4921 a         3270 c         3124 g         5000 t
ORIGIN      
        1 taaactaatg aaaataaaaa aatattcact ttctctaatc actttagcga ttgtagctcc
       61 tcaagtatta gcagaaacaa cctctcaagc agaactaaat gctattgagg tcaaagagaa
      121 attggcagct accaccggct atattcctgt ttatacgact gtaggaacga aaacattaac
      181 ctctttggct cgtacacctt ttagtatcaa tgttgtctct gatgatttaa tgcatgacca
      241 agctgttcgt tctgttaccg attcagtggc ttatacctcc ggtatgatgg gtggctatcg
      301 tggtaacaat tcgcttatcg aaatctccat tcgaggcatt ggcaataaaa gtgatggcag
      361 tacaattccg acctatttaa acggttctcg ctaccaaact gcatttgaaa tcgatccgtt
      421 cttcttagaa agtatcaatg tgattaaagg tccaaactcc attttatacg ggcaagctaa
      481 tcccggtggc gtaatggata ttatcaccaa aaaagccggc agtaacccac atcgccattt
      541 gcaatttgtg gtgggaaacc atcaacgata ccaaatcggg ctggatattg aagaaaatct
      601 ctccaatacc ttatccgctc gtttaatcgg tcaattagaa cgaacaaact ggaataccta
      661 atttgtaaaa gaaaaaggcg gtgcttttgc tccaagcatt ttatggaaac cgactacaaa
      721 tacagaactt aatgtttacg cttactatgc aaaaaaaccg aaagccggtg atcgtaactt
      781 tttaccgaaa gaaggaacga ttaatagcgt aaacgggaag gaactaccct atgatatgtt
      841 cctcagcgat ctgaactatc acggtttaaa agccgaacaa atgcaagtag gttacagttt
      901 ctcacataaa tttacgccaa acctaacgtt ccgccaaaat gcaagttacc ataatggcga
      961 agagaatttc aaaaatctag tttattgggg cgatgtcggt tcggttttag aacgtagagc
     1021 tcgccattgg gatgtcaaaa cccaagaaat caacttagat aaccaattag aatgggtatt
     1081 tagcacagca aatataaatc ataccttgtt agccggtatt gattataaac gagtgcgtaa
     1141 agatcttgat aactatatgg ggattgcccc tgattttgat tggcagaacc ctgtttatgg
     1201 cgtgaccgtt actgagccta gcttattttc aagtgaagtg aagcatttaa aacaacttgg
     1261 ggtttatcta caagatcaaa tagaaatcgg taattttgac ttcctgttag gcggtcgtta
     1321 cgatcgagca aaaaacagca accaagatcg tttgaacagt aaaacagaga ataaccaaga
     1381 aggaaaattt acttggcgta caggcgtagt ttacaacttt gaaaacggta tcgctccgta
     1441 tatcagctac agcacttctt ttgtgccatc ggctgaaaaa gatgctcaag gaaatttctt
     1501 aaaaccaaca actgctgggc aatgggaagt gggaatgaaa taccaaccta cccaagacac
     1561 cttactgacc ttagcaggat ttaaaattac acaaaaagat ttagccaatt atcaatggca
     1621 aacccgctct tatgagcaaa ttggtgaagt gcaaaccaaa ggaattgaag tgagtttaaa
     1681 tcagcaatta tccgataaat tttcaatagc ggcttcttat gcttacttaa aaaaagagat
     1741 tactgcagat aaagatggca cgacgattgg aaaaacgcag tggggagtgc ctcgccatca
     1801 agcatctctt tgggcaaaat ataaattctt tgataagctg accgccggtg taggtgtacg
     1861 ctatatggga acgacttttg gcgataacca aaatactttt accgtaccgg catacacact
     1921 ttacgatatg atgctagctt atgattttat gcctaactta aacttacagc tcaacgcaca
     1981 aaacctgacg aataaaaaat atgtcgcaag ctgtgccaat aatttttcct gtttctatgg
     2041 aaaagatcgc aatttaagtg caacgttaaa ctatcatttt taatctcaac agccctcaaa
     2101 tgagggctta aacttttgca aaaaatatga aaaaaacgac cgcttgtatt cttacttttt
     2161 tggtttcatc tgtgctttat gctaacaata taaccattga gcttaaacag aatgattttg
     2221 tgcaaggcaa attgattcag aaggctcaac agccactttc cttgtcgatt atttttcctg
     2281 accaaagctc tcgcctgctg gctcaaaatg tctttggtga acaagatttt atgtttaaat
     2341 cagaactaat cggcacagct actttttcta ttctagcgaa tggtgcaacc gtttcagacc
     2401 aagattttac cttaacgatt gagcggcata ttccaatctc ggcacaaatt gctccaccta
     2461 aagtaattga aaatacacag cttgaagaac tcagccaaaa aattgcaaaa aatccagaaa
     2521 aacagaccgc tttaattaac gatttttggg ataaagcaaa acaagccggc acaccattga
     2581 ttgaaccatt ggacgaacag caaagccgtg taactttttt atggcgtgga gcaacgaaaa
     2641 atgcccgtat ttggggcggt gtgagtactg aacacgattt tatgcaacgc tttggtgaaa
     2701 cggatatatg gtataaaagt tatatagtgc ccaatgacac tttagtggaa tacaaattag
     2761 ctccagatgt gccgacattg cccgttgatg agaataccca acgcagagcg ttactggcta
     2821 cggctcaagc cgatccactc aataaaacag catacttcga gaaaatcgga caaaatattc
     2881 ataataccga taaattcaac tacgcttcgc tacttaaatt gccaaacgca cctactcaat
     2941 cttttttgac tgaagctcca aatattgcaa aaggtaaact gcagcagttg atttttgaga
     3001 gtgcaacact cggcaataaa cacagattat tcgtctatct gcctgacggt tttagtgcag
     3061 aaaaaaacta tgcggttctc tatttatttg atggtgttga atataccgaa cgagtgccaa
     3121 ctaaaaccat tttagataat atgatcgctc agaaagtgat tctgcctact gtagcggtgt
     3181 ttatcgaaaa tccatcgagt gaatcaagac gtgtcgagct gcctgccaat cccatattta
     3241 cgcaagtatt ggcaaaagaa cttgtacctt ttgttgaaaa acagctcaat ataaaaacaa
     3301 aggagcgaat tattggcggc tctagctatg gcgggctatc ctctgcttac accgtactca
     3361 actatcctaa tgtatttagt aaagcattga ttctatccgg ttcattttgg tggcaaccca
     3421 aaggcacacc tagtgaacaa agccactata ttgctcaaca atttatccaa aaagaaaaat
     3481 taccactctg ttttttcatc agtgcaggtt tttatgaaag cgggaaatcg gctatttgta
     3541 agcggtcgaa ttattttgca tagaattaca ctcaattttg catagataaa aattaaaaac
     3601 tatgcaaaat aattgtagaa gatcaaacct aatctgacag tcccccgttt taaattaccg
     3661 tgtctgtcag attaatttga gcttaaattc ttttccaccc aaacgcgtct attttgattc
     3721 agcaatgctt caacaccacc ttgctcaacc gcttgttgat aacgatagaa tgtatctcgg
     3781 ctcattccca ttactttgca ggcttgagaa atattaccca gttcttctgc taaatttaat
     3841 aaaccgctct tgtgtttaat gagagggttg ttagaataaa acatgagagt ttcctttttg
     3901 tttagattga tttttgacac tcatattcta aacgggaaac tctcactttt tagagtgaat
     3961 tgtcagatca agtctgatct tctacacttt tatattcaat atttttaaaa cgctcatttg
     4021 tttctttagc tgtaatctta ccaagcagat ttaccagatt attacctgcc tcatcgcttg
     4081 taccggcagt gacacgagcc tgttggtttg caattaaaag tcgctcaaag ccctcaatac
     4141 tactcaaacc ggcttgtttt gcagctgcca tttgttgagg caaccaactt gccatatatg
     4201 caagctcaaa actacctgct tgcccggctg caacagccat atccaatgcc cgcccaatat
     4261 cctcttcttt gatgccaaat tgttgcatag aggagatcgc aatttttgcc atatcctccg
     4321 agcttgcccc tgttgccacc gcccctttct gcaaagtagg taatagactc attgcggttt
     4381 cggctttgac tgtaccggag gcaatgagcg tatcaagcgt aactaacgca tcctcttttg
     4441 tgccaccccc aaccttatcc gcctttttat caagtttttg gtttacttct ttgagcgagt
     4501 ctaaaatatc gtccagcttt tccttaagag ccttattcag acagcctatt aagcctaggc
     4561 gtaaaagccc ataattgtgg cgtattttgg gctaatggca catcaatggc cccgactatc
     4621 agcgatggtg atcaaatgtt agtggatttc agcaaaaaag aagcccgtgg agacgataag
     4681 atttatcttg tgcaaaatgg tgaaagcgtt tgggtaaaac gtgtaagaaa agtgtgggac
     4741 tacgttgaat tgattagcga taatgagtcc taccgcccaa tccgaattac agcagacgaa
     4801 gctcaaaact tacaaatcat cgggcaggta gtccacatcg gacactcttt agtttaaaac
     4861 agatttaaaa tagttttaaa accgatcaaa cctatgcaaa aaatatcgca aaaactgccc
     4921 atttttttct aatcctgctc attttgtttt ttgcatagcc ccaaccaaac aataacggcg
     4981 aacccttgta ttcacggggc tcgccgttat ttttccgccc aaattttttt ctttctctct
     5041 atgcaaaaaa gatcagtacc ccacattttc aaccgcttgt agctcacaaa gccaaccttg
     5101 ctctttggca agcagttcaa aatcggtaag gctactctgc tgattgagta aaatttccaa
     5161 gctatcgccc tttttaagat cgttcatcgc tttttttgcc attaataacg gcaaggggca
     5221 ggcatagccg gttaaatcta aaacatattg catagcgaaa tagggaataa agaaaaaggg
     5281 cgaatattga aattcgccca atcacaaaag attagagttt acggcggtct gccatcactt
     5341 tgcccatagc cgataatttg tcttgagcta aatgctctcg cccaagctca aagagtggct
     5401 cttcaatcgc aatatgcaca tcataacctg ccacaaaacg ctcaattaag tctctatcca
     5461 cattttcacg tttacctgcg agcagatctt caagctgtgc cgacaaatag tcccaatttt
     5521 tatgtaaatg aacgtgctgg cgttccagtt cgtctacctc tgcttgagct tgtggttgca
     5581 ccttaataag ctcagggaag aaatcatctt cttcatcttc gtgatgtaaa ggggcagaaa
     5641 tgttgaagta gttcaaaatc tgctgcacgt cattcttcac tgcttggttt accccgtttt
     5701 tctcaaggta atccggcaaa atttgcaact gtttacagaa actttttact ttgctgtggc
     5761 aagcataaag catatcaatc ggctctgccc agcttgcaaa ggtttgtggt tcaagttgtt
     5821 gcatatacac tccttgttat ttctgttctt ttacttcaga ttgtaaaggt ggcacttctt
     5881 tgccaattcg ggcataaaat tccactacaa aatcatcaaa acgatcttct tcgatggcct
     5941 ggcgaatttc tgccattagg cgttgatagt agcgtaaatt atgaatggtg tttagtcttg
     6001 cccctaaaat ctcgccacat ttgtctaagt gatataaata ggctttggtg tagtttttgc
     6061 aagtgtaaca atcacattca ggatcaagcg gtgtggtgtc tgcacgatat ttcgcattac
     6121 gaatttttac aatgccgttg gtcacaaata aatgtccgtt acgggcattg cgggttggca
     6181 ttacgcagtc aaacatatca ataccacggc gaaccccttc gactaaatct tctggcttgc
     6241 ctacacccat taaatagcgt ggtttatctg ccggaatttg cgggcaaacg tattctaaaa
     6301 tgcggtgcat atcttcttta ggttcaccta ccgccaagcc accgactgca tagccatcaa
     6361 agccgatttc aactaatttt tccaccgaga ctttgcgaag ttcttcataa ataccacctt
     6421 gaataatgcc aaataacgca ttcgggtttt caagttcatc aaagcgatca cgagaacgtt
     6481 tcgcccaacg tagtgacatt tccatcgatt tttttgcata atcaaaggtt gccggataag
     6541 gagtacattc atcaaaaatc attacaatgt ctgatccaag atcgtattgg atctccattg
     6601 atttttccgg cgaaaggaaa attttctcac cgctaatcgg gttttggaaa gttacgcctt
     6661 cttctttaat tttacgcagt ttacctaagc taaaaacttg gaaaccgccg gagtcggtta
     6721 aaatagggcc gtgccattgc ataaaatcgt gcaagtcacc gtgcatcttc atcacttctt
     6781 gccccggtct taaccataaa tggaaagtgt tgcctaataa aatttgcgcc cctgtcgctt
     6841 taacttcttc aggagtcatt ccttttaccg tgccataagt gccaacaggc ataaatgccg
     6901 gtgtttcaac gtgaaattcg cctttcgggc ggttaaaagt taaacgccca cgacgagcgt
     6961 taccacttgt tgtttttaat tcgtacttca ttttaatcca caaataaaca gttcgtgaaa
     7021 gcggtcaaaa accaccaaaa atttaccaat tagaatcgaa agtcggtgcc gacccctaag
     7081 ccgattccaa cgccgttaga gccaccaccg gctccaatac tgccacccac accggttgat
     7141 gagcaagcag ctaacactaa aatgccaacg cataataaga gttttttcat tttttgctgt
     7201 cctaaacgaa aaagagagtc tatgatagac ccaatgtatt ctaacctgtt taatggatta
     7261 aacctaattt taaagggtaa attatgaaaa ttttacgtaa attcgctgct gtatgcgttg
     7321 tctcaatggc tatgctctct gctaacgcta atttgctcac tattagcgag caggaaatca
     7381 acgactattt agccaccaaa ttagctgaaa aaattccttt aaaagataaa gtcggtattc
     7441 ctaaattatt tgaattggat tatcgcctgc attcacttac cagccaaatt ggcagaaccg
     7501 aggaaaaaaa agtagctatt tctggcgttg ttgatggact tttaaacgca aaaggcaaga
     7561 aatatgctgc ctctatccat ttaaatatgg ataccacgcc ttattatgat ccacaaaaag
     7621 gggcattatt tcttaaagat gtcagattgt taagttggtc tgcaacaccg gctaaatatc
     7681 aagcagagct gcaaatgttt ttgccggtgt tggctgatgg attaagtagt tttttaaaca
     7741 gtacgcctgt ttatacttta gatgaaaata aaacaaaaga agcaatggtg aaaaagttcg
     7801 gcaaagccat tgtagtagaa aatggtaata tcaagctaga aaccactatt ttttaaataa
     7861 ttttgtgatc ttcgtctaat ttttacactt tgatagttga gcttttacgc gttttattta
     7921 caatccaaat atcgccattc attatttgga gagttctatg gatatcggta ttgttaaatg
     7981 gtttaacaat gtaaaaggat ttggttttat tacatcggat gtatgtgaag gagacatttt
     8041 tgctcatttt tctgaaattc aagcagaagg ttatcgctct ttaaaagtcg gccaaaaagt
     8101 acagtttgaa ttagttcaag gagagcgagg tgcctcagcc tcaaatatca cgcctataag
     8161 cgaaaaaacg ttataatccg tcgttaataa aattattgtt ataaccatac tttattagat
     8221 tttaaagctc agcttttaca gctgagccat ttttgctttt tgaatatgcc ttcctcttat
     8281 caattagtct taattaacct actttatatc ttcggtattg gtctaaatat tgtactgctt
     8341 aggtttctca gtttgcattt tgacccatta aataataacg gtatacgttt tcttgttggt
     8401 gggttcacct tattattttt cattttatgg agattccgca catcgctgtc tcagctgatt
     8461 aaaactccaa aattgattgc gatttctcta tttgtcgggt taatgatgtc cgcaaatatg
     8521 tatttttggc taaaaggggt caaaattaca aatgctgtta ccgtctctat ttttggggta
     8581 ttagctatgc catttggtgt gattattgcc gccatattct ttcaagatga aagagaacgc
     8641 attaaaagca aagctttctg gttcggctca attttaacga tcatcggttc actcggattt
     8701 atttggcacg ggaaaacact ctcgattggc gaaggattta ttcttggttc tgtgttctta
     8761 tttttgtcga tcattatccg taatatacaa aacctagttg taaaatttgc tcgagaactg
     8821 aacgtattaa ccctaagttg tgtaacatct ttaactactt cactctcgag tctattactt
     8881 agccaacaag caggtaaatt taatgagctt tttactgcac caaactggct actattctct
     8941 ctgatggttg ctggtattta tgcgattatg atcggaatgg tattaacctt tcacattatc
     9001 caaacccaag gactgataac ctaccaagta ttggaactat taatgccggt ttcaacagcc
     9061 atcattgctt atattttact ggacgaacgt atttccattg ttcaaatggg ctttgcttgt
     9121 attgtgattt taggctcaag tatcgcctta aagttagtaa aatatcctaa gcactattag
     9181 gatctttgct aattaatatt ggtaaaatta catttggttt actaatctga gcgttcattc
     9241 attagtaatg ataaacaagc ggttattttt tgttattttt tggtaaatac tgatagtcat
     9301 gtataaaaac ctttctttgc tttcaccgac ttatccattt gaccttgaat tcatcgccgg
     9361 tcttactcaa tgtgagaaag gtaactatct tgattctatt gtcgataggc aacaaggaat
     9421 gcacggttat atgcttcaat taaccacttt tggtcgtggt tctgtttttg atggtaagca
     9481 cgtttttacc gctcatcgag ggcagttact cctctttaca cccaatgccg ttcaacacta
     9541 ccaccgtcac ccggaaagcc aatattggca ctacaaatgg gtctattttc agccgaatcc
     9601 taaatggaat aaatggctga attggtcaga tatggaagaa aaaataggac gtatcaccat
     9661 tagcgatgat cgttactttc aggaaattag ccaacttttc agtaaaattg aattagaact
     9721 taaatccagt aaatttttta aagaagatat tgcaacaagt ttgttggaat acttacttat
     9781 gaaatgtatt tccgccgaga aaattgaggt tattcctgtc attgatcaac gcattttgac
     9841 agtttgtgat ttaatcttaa cgaatttggc taaaaatgag agcttagaag caatggcaga
     9901 aaaagtattt ttatcgccat ctcgcctctc acatcttttc aagcaatcgc tggggatcag
     9961 cttagtgcaa tggcgggaat tacaacgcat ttccgaatcc aaaaaattgc tttatttctc
    10021 taacatctct atctctaata ttgcgaaatc cttaggctat gaggattctc tctacttttc
    10081 caaaattttc aaaaagcata ccgggctgtc accctctgag tttagaacct tagaacggcg
    10141 taaagacagt gaaatgtgat cttgatctca ctttactttg catagcagaa ttttccatat
    10201 cgataacaaa aagttacctg tttttctctc attcaaaagc ataagataca aacagcttta
    10261 atagtgctgt ttctattaga gaagggatat caagctctgt gagtgatatt cagtgaaata
    10321 acttcttatg tatgagggaa gaacaatgaa aaaaccaacg ttcaagcacg cattattttc
    10381 aagtatttta gctttagcct gttacggctc ggcatttgcc aatgatgttg ttaatatctc
    10441 caaaattgac ggtatgccgt ggtttaatcg tatggcagag ggtgttgtgc aagcaggcaa
    10501 agataacaat atcaatgctt atcaagttgg tccatcaaat accgatgcac cacaacaagt
    10561 aaagttaatt gaagatttaa ttgcgaaaaa agttagtgct atttccatcg tccctaacga
    10621 tgcaaatgcc ttagaaccgg tactgaaaaa agcgaaacag aacggtattg tggtattaac
    10681 caatgagtca gtcggacaac ctagtgccga ttgggatatt gaaattatcg ataatgcaca
    10741 atttgcagca gactatgtag aagaagtagc aaaagctatg ggcggaaaag gcggctatgt
    10801 gatttatgtt ggtggtttaa ccgtgcctca acataatctg tgggctgatt tattcgtgaa
    10861 ataccagaaa gagcattatc cggatatgtt tgaagtgacc agcagaatgc ctgttgccga
    10921 aaatattaat gatgcacgcc gtaccaccat tgatttagtg aaagcccatt ccgatcttaa
    10981 agcggtcgtt tcttttggtt cacaaggtcc tattggtgca gggttggcag tgaaagaaaa
    11041 acgcttaaaa aataaattaa atgtcttcgg aatgatgatt ccttcccaag cagccaattt
    11101 aattaaaagc ggtgatatta caatgggaat tacctatgac ccggccaatg caggttatgc
    11161 tttagctgct gtcgctgcca aagtattaaa aggcgaaaaa attgaggatg gattagagat
    11221 cccaacggtc ggtaaagcca aagtagatag tgagaaaaaa ctacttcaat tccacaatgt
    11281 gctgaaagtc accaaggaaa atatcgataa attatattaa tttgcttgaa atatgggctg
    11341 atagccagcc catattttcc ctcaggaggc acttatggca tttatttcga tgcggcatat
    11401 cagtaaaaca ttccacggca taaaagcatt aaatcaagtc aatctctcat tagattatgg
    11461 cgaagcactt tgtcttgccg gtcaaaatgg ctgtggaaaa tcaaccttga tcaaaattct
    11521 ttccggcgtt tatcaaccgg ataaaggggc tgaaattcaa attggtgcaa cccaatatac
    11581 caagctgaca ccacaggaat cgattgataa aggtattcaa gtgatttatc aagatttagc
    11641 cctcttcccc aatttaaccg tggcagaaaa tattgccatt aacctgcacc gaaaatttgg
    11701 ctgggtgagt caagatcaca ttcatcaggt tgccctcact gctattcaaa gcattaatgc
    11761 aaatctagac ctgaatgcaa tattagaaga tttaccgatc gctcaacaac agttagtcgc
    11821 tatttgtcga gcgttagcac aaaatgccag attattgatt atggacgagc cgactgcctc
    11881 acttactgca aaagaggtca aagatctgtt agatgtcgtc atcaaactta aaagcaaagg
    11941 cattagcatc atctttgtca gtcataaatt gcaagaagta atgagtgttt ccgatagcgt
    12001 attagtgctg aaagatggca atatggttgg gcaatatcct atccgtgaaa tgaatgaaaa
    12061 acgccttggt ttcctaatga ctgggctgga aatcgattac aagcggttag atttgccgga
    12121 ttttttgcaa agaaaaaccg tattggaagt ccgcaattta acgctgcctc atcagtatca
    12181 agagattaac ttttcgctac gagaagggga aattgttgcc cttacaggat tgctcggttc
    12241 agggcgtact gagctttgcc taagtttatt cggcattact caaccacaat ccggcgatat
    12301 tttattaaac ggtgaaaaag tgatatttaa cactaaccgt gatgccattg ccaaagggat
    12361 tgcttatgta tcggaagata gaatgcacac cgggctgatt atggcagagt cgattcatca
    12421 caatattatt tcgactattt ttcacaaaat caccgataaa ttacagatta ttcaatcctc
    12481 gaaggcttat gcaagtagcc aagccttgat tgattcatta aagattaaag tatccgattc
    12541 agatttgcct gttaataccc tttccggagg gaacgcacag cgagtttcga ttgcaaaatg
    12601 gctggcaatc gaccccaaaa ttattatttt agatgccccg actattgggg tcgatatcgc
    12661 caataaagaa gggatcttcc aaattattaa atcattggct cagcaaggca ttgcagttat
    12721 ttttgtgact gatgaagtgg aagagaccta ctacaacagc catcgggtaa ttgtaatgaa
    12781 aaaaggtcgc attgttgatg aaatcttgcc ggcatattcc agtgaaaaat cgattgcgga
    12841 ggttatttat gaaaatcagc aataatcata ttctttccag tacgttactg attttaatcc
    12901 tattattaac cctatttact cgtgattttg caactattga gaacctttat gatgttgtaa
    12961 ataattacgc aatgttaatg attttagctt gtggtttatt tgtggtattg ctgtctggcg
    13021 gcattgatat ttctttccct gctatcacta ttatttccca atatgttatg gtcactctga
    13081 tcggtaaata tgatgtagga tttgccggcg tttttgttat ttcttccacc atcggcatat
    13141 tattaggttt tattaatgct ctatttgtta atcggcttag tgtcccttcc attattatta
    13201 cgatctcaac actcaatatt ttttatggcg gactactcta ttttaccaaa ggtgtatggc
    13261 tttatgagta tcccgaatgg tttacccaag aaatcgtgtt ctttaaacag ataactgctg
    13321 agggagtgga atttggatta tcgtttcaag cattaatggc tattttggcg gtgatattaa
    13381 ccggcttaat taccaataaa ctcactattg gtcgaaaaat ttacgctctt ggtggtaatc
    13441 cggatgcaac ctcccgtatt gggtttaatc tcttatttct ccatctattt gcatacggtt
    13501 atatgggctt tatggcaggc ttggcaggca tagtgcaatc ttataccgta caatctgtcg
    13561 caccggattc actactgggt tatgaattaa ccgtcctagc tgccgttgta ttaggtggtg
    13621 ctagtttatc aggtggtaga ggcaccctgc ttggtacagt aatgggcgta atgttactgg
    13681 cgattattca aaacgggcta aatttaatga atgtatcgtc ttactggcaa accattatta
    13741 ccggtatcat cattatctta agtattagtg caacagcctt aagtcagcgg aaaaaacaag
    13801 gagcataacg atgaaaagta aactggatcc aacgattttc tatctcttag cactcttcgc
    13861 agttgcagtt atcgccttta gtattttcat tccggatatt ttttggtctg tcagtaactt
    13921 tcaatctatg gcatcacaaa ttccagtatt aggtatttta accctagcaa tggcattacc
    13981 gatgttaaca ggtggaataa atctttccat tattgccgga atgaatgctt gctcattaat
    14041 tattgcctac tttattacgc aatataccgg tatttcttgg ttatttattg ctattttatt
    14101 aagcctttgc tgtgccttag ccattggatt actcaacggc acattaattg ccattattcg
    14161 tgtttcacca attttggcaa ccttaggcac aatgacttta attaacggca ttaatattct
    14221 agtcactaac ggttcaacca ttgcgaactt tccggaaaca atgttgagta ttaatgcttc
    14281 agccttttta tggattccgc tacccattct aatctttctc tttcttgctg ggctgatttg
    14341 gtttttctta gaaaaaactg caatgggtaa agcggtttat ttcatcggca ataatgaaaa
    14401 agccacctat tattcgggta ttaataccaa aaaagtgctg atttgggttt atgttatctc
    14461 ctcaattctt tgtgtatttg cagctatttt gatgatgtct aaactcaatt ctgcaaaagc
    14521 ctcttatggt gattcctatt tgttgatctc cattttagcc gcagtattgg gcggtattaa
    14581 ccctgatgga ggtaaaggga aactattagg gattatgctc gccttagttt tattacaagt
    14641 gattgaaagt ggcttaaata tgctcggcat tagcagttac attacaatga ttttatgggg
    14701 aagcttattg ttattcttta tcttcctcca aaaaaatcgc cttttccgac taacacagta
    14761 atggtggtag attatgcaaa atgaaaaaca actcattgaa caaggtaata ctgttattgg
    14821 tattgaattg ggctcaaccc gaattaaagc ggtgttaatt agctcagatg ggacgatttt
    14881 agcaaccggt ggggcggatt gggaaaatcg actgattgac ggtatttgga cctatcatca
    14941 aaatgaaata tgggaaaaac tacaagctgc ttatatggat ttaagcaaga cagtcaaaga
    15001 aaaatatcac acaaccattc gcacagccaa agcattggga attagtgcga tgatgcacgg
    15061 ctacttacct ttcgataaac acggaaatcc gctggttgcc tttagaactt ggcgaaacaa
    15121 cattacccta acctcatcgg aaaaattgac cgcactcttt caatacccaa ttccgcaacg
    15181 ttggagtatt gctcatttac accaagcatt gcttaaccaa gagcctcact tgccggaaat
    15241 tgattacctg acaaccctct caggttatgt gcattggcaa ctgaccgatc acaaagtgtt
    15301 agggattggc gatgcttccg gcatgttccc gatagatact gagttgaaat cctacaatcc
    15361 aacaatgctg gctcaatttg atcgagccat tgccgactac cgtatgcctt ggaaattgga
    15421 agcgattttg ccacaagttc gtgttgctgg tgaacaagcg ggcaaattaa ccgaaaaagg
    15481 tgcaaaattg ttagatccaa ccgataattt gcaagctggc attccacttt gtccacctga
    15541 gggtgatgcc ggaacaggta tgattgcaac caactgtatc aaagaaaaaa ccggtaatat
    15601 ttctgccggc acctccgcat ttgcaatgat tgtgttagaa aaagccctat ccaaagccta
    15661 ttcagagctg gatattgtga cgaccccggc aggtaaatta gtggcaatgg ctcacgctaa
    15721 taattgcacc tctgatatta atgcgtgggt gaatttattc ggcgaatgct tatcggtttt
    15781 tggcgtaaat atctgcaccg aagaacttta tgaaatgtta tttctccatg ccttgcaagg
    15841 cgatgcagat tgtggagggc tattatccta cggcttccac tccggtgaac ataatgtcgg
    15901 tttatccgaa ggctgtccgg ttttcttgca tccaacgaat gcccatttta acttagcaaa
    15961 ctttatgcga acccatctct ataccgcttt tggtgcaatg aaattaggaa tggacattct
    16021 cattaaccaa gaaaaagtgg aaatttcccg tattctcggt cacggtggta tatttaaaac
    16081 agaaggtgtt gcacaaaaaa ttctgtcttc cgcactgaat attccattgg ctaccgcaag
    16141 tactgcttca aatggcggag cttggggaat tgcattactg gcagactttc tcagcgtatc
    16201 agaccgttac agtttagaag aatatttaga taattgcatt ttcaacacca ccaaactcaa
    16261 tctggttcaa ccggataaaa caatgagtga aggttatgaa cgctttatgc aacgt
//
LOCUS       DANLUA010000035        16290 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLUA010000035 DANLUA010000000
VERSION     DANLUA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16290
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            296..877
                     /gene="rsxA"
                     /locus_tag="PXB44_000852"
     CDS             296..877
                     /gene="rsxA"
                     /locus_tag="PXB44_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL5281353.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
     gene            877..1494
                     /gene="rsxB"
                     /locus_tag="PXB44_000853"
     CDS             877..1494
                     /gene="rsxB"
                     /locus_tag="PXB44_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL5281354.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            1481..3775
                     /gene="rsxC"
                     /locus_tag="PXB44_000854"
     CDS             1481..3775
                     /gene="rsxC"
                     /locus_tag="PXB44_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL5281355.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            3768..4403
                     /locus_tag="PXB44_000855"
     CDS             3768..4403
                     /locus_tag="PXB44_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL5281356.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            4449..5504
                     /gene="rsxD"
                     /locus_tag="PXB44_000856"
     CDS             4449..5504
                     /gene="rsxD"
                     /locus_tag="PXB44_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL5281357.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            5504..6136
                     /gene="rsxG"
                     /locus_tag="PXB44_000857"
     CDS             5504..6136
                     /gene="rsxG"
                     /locus_tag="PXB44_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL5281358.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            6145..6828
                     /locus_tag="PXB44_000858"
     CDS             6145..6828
                     /locus_tag="PXB44_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL5281359.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            7269..8219
                     /gene="lpxM"
                     /locus_tag="PXB44_000859"
                     /gene_synonym="msbB"
     CDS             7269..8219
                     /gene="lpxM"
                     /locus_tag="PXB44_000859"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL5281360.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            8347..8886
                     /gene="apt"
                     /locus_tag="PXB44_000860"
     CDS             8347..8886
                     /gene="apt"
                     /locus_tag="PXB44_000860"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL5281361.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            8977..11034
                     /gene="dnaX"
                     /locus_tag="PXB44_000861"
     CDS             8977..11034
                     /gene="dnaX"
                     /locus_tag="PXB44_000861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL5281362.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            11040..11948
                     /locus_tag="PXB44_000862"
     CDS             11040..11948
                     /locus_tag="PXB44_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5281363.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            11950..12711
                     /locus_tag="PXB44_000863"
     CDS             11950..12711
                     /locus_tag="PXB44_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281364.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            12732..13487
                     /gene="modA"
                     /locus_tag="PXB44_000864"
     CDS             12732..13487
                     /gene="modA"
                     /locus_tag="PXB44_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5281365.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            13671..14363
                     /gene="modB"
                     /locus_tag="PXB44_000865"
     CDS             13671..14363
                     /gene="modB"
                     /locus_tag="PXB44_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL5281366.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            14350..15405
                     /gene="modC"
                     /locus_tag="PXB44_000866"
     CDS             14350..15405
                     /gene="modC"
                     /locus_tag="PXB44_000866"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL5281367.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            15460..15894
                     /locus_tag="PXB44_000867"
     CDS             15460..15894
                     /locus_tag="PXB44_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL5281368.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            15884..16219
                     /locus_tag="PXB44_000868"
     CDS             15884..16219
                     /locus_tag="PXB44_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL5281369.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
BASE COUNT         4590 a         3419 c         3728 g         4553 t
ORIGIN      
        1 gtgagacttt ttaggattaa cttagaaaat caagctcctt tcacctttca tgctgtatag
       61 cttagtcccc acattctcca ttctaccaga gatagaaaca aaggtaacaa gatcatattt
      121 gatatggtat gtttactaga agtacctccg atttcttatt gtgaatgagc cgaaaacaag
      181 agataatata acgtttattt ttaatgagat gctttaacaa acggtcagat tttctctgtt
      241 ttttgcaaaa taaaatgcgt ttttgaccgc ttgtatattt aattttgtgg aatttatggt
      301 cgattatatt ctgttggtca tcagcaccgc ccttattaat aactttgtct tagttaaatt
      361 tctagggctt tgtccgttta tgggagtgtc gaaaaaggtg gaaactgcta ttggtatggg
      421 gttggcgaca acctttgtgc tgacggttgc ctcgctctct gcttatttga tggaaaccta
      481 tttgcttgag ccgttaaacg ctcaattttt gcgtaccttg gtgtttattt tagtgattgc
      541 ggtgatcgtg caattaacgg aaatgattgt gcataaaacc agcccgacac tttatcgctt
      601 gcttgggatc tatttaccgc tgattaccac caactgtgcc gtattagggg tggcattatt
      661 aaacgtgaat ttagccaata acttggtgga atctgttctt tatggttttg gagccgctgc
      721 cggtttctcg ctggtgttag ttttatttgc ggctctgcgt gagcgtttgg ctgccgctga
      781 tgtgcctcgt ccgttccaag gagcatcaat tgctctgatt accgcaggct tgatgtcgct
      841 tgcctttatg ggctttacag gattggtgaa actctaatgc ttgatttgcc aattatcgct
      901 tatattctta tcgctattgc tgtgatcgca ctgattttcg gggcggtttt aggctattcc
      961 tcgattaaac tgaaagttga atccgacccg attgtcgatc aaattgatgc ccttttacca
     1021 caaagccaat gcggtcaatg tggctacccg ggctgtaagc cttatgctga ggcgattgct
     1081 aatggtgatg tgatcactaa atgtgtgccg ggtggacaac ctttagtggt gaatcttgcc
     1141 gaattgcttg gggtagaagt gccgccaatg gaaggtatgg cagagcctga gatgaaagtg
     1201 gcttttgtga ttgaagatct ctgcattggc tgtactaagt gcattcaggc ttgcccggtt
     1261 gatgcgatta tcggcacgaa taaagcaatg cacaccatta ttccggatct ttgcaccggc
     1321 tgtgaacttt gtgtcgcccc ttgcccgacc aactgtattg aaatgcgacc aattaaaccg
     1381 acaacccaat cttggaactg gaaatttgac caagatttaa ttattccgat tgtgaatacc
     1441 actgaattgc agagaaaatt agttaccggc ggaggcaaaa atgagcaatc ctaatttagt
     1501 gattgagcga atccgccaaa ataaagaaac gggtaaactg tgggatttcc cgggcggtat
     1561 tcacccgatg gaaaataaat cccaatcaaa cagcaagcca attcgcacct taaatttacc
     1621 gaagtttttc tatgtgccac tggttcaaca ttctggctgg gcaggggaat tattagtcaa
     1681 agtcggcgat gtggtattga aagggcagcc gctcacgaaa ggcgatcact tccgtcaact
     1741 gcccgtacac agcccaacct ccgggcgggt gattgggatt gagccttatg tttccgctca
     1801 cgcctcaggt ttgcccgaaa taacggtgat tattgaaacc gatggtaaag ataaatgggt
     1861 ggaacgccag ccgattgagg attttttaag cctaacaagc gatcaaatta tccaaaaagt
     1921 ttaccaatat ggcattgccg gtttaggcgg tgcggtattc ccaaccgcaa caaaattaag
     1981 tttggcggat aaacgctgta aattactggt gattaacggg gcagaatgtg agccttacat
     2041 cacctgtgac gatcgcttaa tgcaagattt cactgcggat cttatcgaag gtattcgcat
     2101 tctgcgttat gtgcttcgcc cggaagaagt ggtgattgcg attgaagaca acaaaccgca
     2161 cgctattcaa gctttagaaa ccgctttaaa aggctcgaac gatatgtttg tgcgggcgat
     2221 cccgactaaa tatccctccg gagcttctga tcagctgatt caagtgttga ccggacttga
     2281 gattccggct ggcaaacgca ctattgaaat gggcattgtg atgcacaatg tcggcacggc
     2341 gtttgcggtt aaacgggcaa ttattgatga tgaacctttg attgaacggg tagtgacgct
     2401 gacaggcgat aaaattcgca acaaggggaa cgtgtgggca agattaggca cgccaattca
     2461 gcacttgttg gagcaggtag attatcaagc cgataaacgc tttccggtgt ttttaggcgg
     2521 accgatgatg ggctttattt tgcctgcatt gcaagccccg attaccaaaa ctgcgaactg
     2581 cttgattgcc cctgatcatt ttgaatatgc accacctgaa ccggagcgta gttgtatccg
     2641 ctgttcaagc tgttccgatg cttgcccggt ggggttattg ccacagcaac tttattggtt
     2701 tgcccgtgca gaagatcacg ataaatcgaa agaataccat ttagatgcct gcattgagtg
     2761 tggcgtttgt gcttacgttt gcccaagcta cattccgtta atccaatatt tccgccaaga
     2821 aaaagcaaaa atcgaggaaa ttgaggaaaa agcccgcaaa gcggaagagg ctaaaatccg
     2881 ctttgaggcg agagaagctc gcttacaaaa agaacgagat gccagaactg ctcgcattag
     2941 ccaagcggcg gaaaaacgcc gtgaagaagt ggcaaatgca ggtggtgttg atcctgtaaa
     3001 agcggctctt gaacgcctga aagcgaaaaa agcggttgaa acagtccaag aaataccggc
     3061 agaagtacag gtaaaaccta acggcgagcc tgataacagc gatttaatgg cacaacgcag
     3121 agccagacgt ttagccaaac aggcagaaca agcggtcgaa aaatcggaaa atgttacaaa
     3181 tgccttgatt ggggcaaata atgatttgcc tctacaatca atgaacgaaa ctgcatcgga
     3241 taaatctgta ggggcgaata actattcgcc cgaagcagaa agcgaagacc cacgcaaagc
     3301 cgccgttgcg gctgcccttg cccgagccaa agccaaaaaa caggcacagc aagcggtcga
     3361 aaaatcagaa aatattacca atgccgtgat tggggtaaat aatgatttgc ccctacaatc
     3421 aatgaacgaa acggtatcgg ataaatctgt aggggcgaat aaccattcgc ccgaaacaga
     3481 aagcgaagac ccacgcaaag ccgccgttgc agcagctatc gctagagcta aagcgaaaaa
     3541 acaagctcaa caagcggtcg aaaaaccgga agatattgcc aatgctgtga ttggggtaaa
     3601 taatgatttg cccctacaat caatgaacga aactacatcg gataaatctg taggggcgaa
     3661 taaccattcg cccaaaacgg aaagcgaaga cccacgcaaa gccgccgttg cggccgccat
     3721 cgccagagct aaagcgaaaa aagccgctca gcaggagaaa agcaacaatg tttagcgagc
     3781 taattccggc attgccgtgg tcgttcctgt ttattcagga cttaatcggc actatcgtgt
     3841 ttgcgatttc gggggcaatt gctgcccgcc agcacaaaat ggacattttc ggtatgttta
     3901 ttttagcgtt tgtgaccggg gtcgggggcg gtacgctaag ggatatgatg attggctcca
     3961 cgccggtgtt ttggatgaaa caaccgatct atgtgctgat gattaccctt gcagtggtga
     4021 ttaccgctat tttccgcaac caaatttccc gcaaaagttg gcaaaccggc ttgctggtgt
     4081 ttgatgccat cgggcttggc gtatttaccg tgattggcgt gcaaaaaggc ttggatttcg
     4141 gcttgcaccc actgatctgt gtggctctgg gcggtgtgac aggctgtttt ggtggcttaa
     4201 tccgtgatat tttacgcaac gaagtgccga tcattttgca aaaagaagtg tatgtgaccg
     4261 ccagccttat cggtggtgtg atttttgtgg ctgcaaactc agttgggctc gaaagcgatt
     4321 gggtttacct tgccaccgtg ttcaccgtaa ttattatccg cctgctggca atacgcttta
     4381 atttgaattt gccgagactt taataagcgg tcattttttt gcaatttttt actaaaaagg
     4441 ttttatagat gtttaaaatg gtcagttccc cccacactca ttcgagcaat ctcactgcga
     4501 attttatgct gtgggtgatt gcgatgatgt tacctgcttt tggggttcag ctttattatt
     4561 tcggctatgg tgtgctgatt caaacgttgc ttgccgttag ccttgcgatt gtgattgaaa
     4621 tcgggattgc aaaattacgc aaaaaaacaa ccgctttcta tttggctgac ttatcaggta
     4681 ttttaaccgc cttgattttg gcgatgtcga ttccgcctta tgctccttat tggcttgtgt
     4741 tgattggcgt gattgtggcg ttattattgg caaaacacag ttatggtggc ttggggcaga
     4801 atctatttaa cccggcaatg gttggctatg cgttgttgtt ggtttctttc ccggtgcaaa
     4861 tgacggcgtg gatagtgccg attgagttat taagtgaacc accgacattt cgtgatgcga
     4921 tttcgttgat tttttacggg gtaactagcg atggttttag tattcatcag ctagttaata
     4981 gtgttgatgg cattgcacaa gctaccccgc ttgatagtat gaaaagcagc cttgcaaaat
     5041 caggttttga cgcggtaatg aatacgccga ttttcaacgg gctgtttgcg accggttggc
     5101 tacaactgaa ccttgcgttc ttattcggcg gtttggtgtt gatttataaa cggattatcc
     5161 attggcaaat tccggtagca atgttggcaa cttttgcctt ttttagtgga ttcaccgagc
     5221 tgtttagcca acaatcgcac ttgcattttg tgagccattt ctttagcggt gcgatgatgt
     5281 tcggggcatt ttttattgcg accgacccgg taacagcctc cattacccca agaggaaaac
     5341 tgattttcgg tggcttggtg ggattattgg tttatctaat ccgctactac ggaaattacc
     5401 ctgatgcgat tgcattttca gtgttattgg caaacatttg cgtaccgtta atcgaccatt
     5461 atacgcagcc gagattatac ggcacaaaat tggataacag ataatgaaca cctcaaaaat
     5521 taccttaaat tacgccttaa ttttaggctt tattgccttg ctttgcaccg ctgtttcaac
     5581 cggtgtgtac ctgcttacca aaggcaagat tgatgatgta accgcaatgc aacaacgcaa
     5641 attattggaa gaggttgtgc cgaaaagcca ttttgataat gatgtgttgg cgacttgcaa
     5701 actggttcag ctcgaaaatg ccccttattt gaaccgtatt tatgttgcaa aaaaatcgga
     5761 aaatctgacc gcttacctag ttcaaggaac ggctccggac ggctattccg gcgatattgt
     5821 gctattgatg gggattgagc cgaacggcaa cattttaggc gttcgcaccc ttgagcataa
     5881 agaaactccg gggctgggag ataaaattga gacccgagtg tcggattgga tcttatcttt
     5941 taccaatcaa ctttttagtt tagaaaacga agcggattgg aatgtaaaaa aagatggcgg
     6001 caagttcgac caatttaccg gtgcaactat tacacctcgt gcggtggtca atcatatccg
     6061 ccaaagtgcg aaatgggtgg tgacagagct ggctcaaaac ccgacaatgt ttaatcaatt
     6121 taaagcgtgc aaataggggg agaaatggaa agagaacaac aaatccctgt gacgaatatt
     6181 gacgttgccc cgaaacaatc cagcgtttgg aaaacgattt tcgctgatgg tgtgtggaaa
     6241 aataatggtg ctttggtgca attattaggg ctttgcccac tactggcggt ttcaaacagc
     6301 gtaaccaatg cacttggact aggcttggca acaatgtttg tgctaatttg caccaatatc
     6361 acggtttccc tgtttcggaa aatcactcca cacgatattc ggattccgat ttatgtgatg
     6421 gtaattgcaa cggtggtaac ggcagtacag cttttaatga atgcttttgc tttccctgtt
     6481 taccaatcgc tcgggatttt tattccgctg attgtaacca actgtatcgt gattggacga
     6541 gcagaagcct atgcctcaaa aaatgaggtc aaattctcgg cttttgacgg cttttcaatg
     6601 gggctgggaa tgacttttag ccttgtggtg ctaggcgcgt tgcgtgaaat tattggcaac
     6661 ggcacgctgt tttcaggctt agatctactc cttggcgatt gggcaaaagc cctacgcatt
     6721 gatgtattgc atattgattc aggtttacta ctggcaattt tacctccggg ggcttttatt
     6781 ggacttgggg tgatattggc ggtgaagaat attattgata agagatgatg tgttatgtca
     6841 cttttctttg cttgtgcaaa gaaaagtaac caaaagaaag cacaccctgc tttgttgcta
     6901 tcctcacttg gttgaaattt tcttaacgaa aaataaccct gatgttcgct tcgctctcgc
     6961 tcgagttatt ttcctaaaaa tttcaagtcg ttcgggcaac tcagaaggga acccgatccg
     7021 accatacaag cggtgatttt tttggaaaat tttgcaaaat gatgctctgc cgggttccct
     7081 tctgaaccgc ccgaacgact ttgaatttgt aggaaaaatt ccttgtttga gcagcgaagc
     7141 ggcgagtttg gaattttttc gttaagcaaa ttcaaaccaa gcgaagatag cggtgaagta
     7201 gggttgcctt tctttggtta ctttctttgg cgaagcaaag aaagtgacaa ataatgagga
     7261 attatagaat gacacgaatt accgcccaag acggctatac gcacaaattc ctatggtctt
     7321 ttctacaccc gaaatattgg ggcgtatggt taggtgtatt aggattagtg attttagcct
     7381 atgtgccggt gcgaattagg gataaattat ccgcttttgt gggtaagatg gcgtatcgct
     7441 atctcaagca gaaaaataaa aaaggctacc accgtgctaa agtgaatttg cgttactgtt
     7501 ttcccgattg gagtgagaaa aagcgtgaag aagtggtcga gaaaatgttt atcacggtag
     7561 cacaaaccat gttggcgatt ggcgaaactg caatccgccc ggcctcttat ttacaaaaac
     7621 ggtgtgaatt taccggcttt gaaaatgtgg tgaaagccaa agagagcggt aaaaacgtga
     7681 ttatgttagt gccacatact tggtcgattg atatggcggg ggtggcgatg ttctcgctcg
     7741 gttatccgat gacatcaatg tacaatccac accgtaatgc gttggtggac tggctgtgga
     7801 atattacccg tgagcgttta ggtgggcgaa tgcacactcg tcaaaatggg attaaagcgt
     7861 ttttagccga tgtgcgtaaa ggtgatgttg gttattttct gcccgatgaa gatttcggcg
     7921 aagagctaag tgtgtatgcc gacttttttg ccaccgaaaa agccactttg ccgggcttaa
     7981 ataaaatggc aagagtggca aatgcggaag tgatcccaat gttctcgatt tataatgcgg
     8041 aaaaagcaaa atatgaaatg gaaattttgc caccgcttga attttcagga gacccaattc
     8101 aatccgcccg agaaatgaac cgagtaattg aatattttgt ggagaaaaac cccgagcagt
     8161 atgtttggat tttacgtttg cttaaaacca gacgaaacag ggaagatatt tatctataat
     8221 atagggaaaa ggaaactgaa aagtaagctg tactatcaaa cgtaaggtta tcaatttctc
     8281 cttgaaattt ctgctgtttt tctatagagt tttccagtta tttttttatt acacgtaggt
     8341 caaacaatga accaattaga tttaattaaa tcttctatca aatccattcc agattatccg
     8401 aaagcaggga ttatcttccg tgatatcact tcacttttag aagtgccaac tgcattccaa
     8461 gccaccattg atgccatcgt tgcagaattt aaagacaaag gtattactaa aattgtcggc
     8521 acagaatctc gcgggtttat ttttggtgcc cctgttgcat tagcgttagg cgtgcctttt
     8581 gtgttggttc gtaaaccgaa aaaattacca cgtgaagtga tttcgcaatc ttacacgctt
     8641 gaatatggtg aagacacgct agaaattcat aaagacgcag tgagtgctaa tgataatgta
     8701 ttggtcatcg acgatttact cgcaaccggt ggtactattg atgcaactgc aaaactaatt
     8761 cgtcgcttag gtggtaacgt agaacacgct gcctttgtga tttggctgcc tgagttaggt
     8821 ggtaaagagc gtttggaaaa agaaggggtt aaatccttta gcctagtcag ctttgaaggt
     8881 cattaatggg gaacaagcgg tggctttttg ctaaaatttt gcaaaatttt tcggaaagat
     8941 gaccgcttgt gatttccaaa ttgaatagaa aaatcaatga gttatcaagt tttagcccgt
     9001 aaatggagac cgcaacgctt ctcggaagta gttggtcagc aacacgtttt atctgctctt
     9061 gaaaacagct taagagaaga tagactgcac cacgcttacc tgttttcagg cactcgtggt
     9121 gtgggtaaaa cctctattgc ccgtttattc gccaaaggct taaactgtgt aaacgggatc
     9181 accgccgagc cttgcggtga atgtgcgaat tgtaaagcga ttgaagaagg gcattttatc
     9241 gatctcattg aaattgatgc tgcttctcgt actaaagtgg aagacacccg tgagctactt
     9301 gataacgttc aatacaaacc caccgttggg cgttttaagg tttatttaat tgacgaagtg
     9361 catatgctct ctcgtcatag tttcaacgcc ttactaaaaa cccttgaaga gccgcctgaa
     9421 tatgtcaaat tcttgctggc taccactgat ccgcaaaaac tgccgattac gatcctctct
     9481 cgctgtatgc aatttcatct gcgggcatta gagcaatcgc aaattgcccg tcatcttgaa
     9541 tttattctaa atgcagagaa cattccgttt gagcctttgg cactagaaaa gttggcaaaa
     9601 gcagcacaag gtagtatccg agactctctc agcctcaccg atcaagccat tgcaatgagt
     9661 aatgccaata ttacgcttgc gatagtcagc caaatgctag ggttgattga cgataatcaa
     9721 ccactagaat tagtgcaagc aaccgcccaa gccgacggtg aaaaagcaat ggcgatcatc
     9781 gagcaaattg cagcgaaagg cgttgattgg caacaattat taagcgatgt ggcagaagtg
     9841 ttacacaaaa ttgcaatgtt gcaattagtg aaaacaccta caaccgaaga aacattagtg
     9901 cattttctgg caaaacaaat tccgcccgaa gacgtgcaat ttttctatca attaatcctc
     9961 aacagtaaaa aagagttgcc ttttgcaccg gagcaacgtg ccggtgttga aatggcgatt
    10021 ttgcgtgcgt tggctttcca ccctaaacag gcagaaaaaa tagcaacagt tgcagtggaa
    10081 aaggttacac aaccaaacca ttcggtttca gcggttacga cagcaaaacc acaaaatcaa
    10141 attagccagc taagagaaaa ttttgagcaa aatcagcaaa aaacggttac tgttgccaat
    10201 actaccacag cggttgaaag cattgctcaa ccgccacagg cagtagatac cgctatttcg
    10261 ccgtctgctc ctgcgactct ttcaccggca atggcagccc tcgaagcccg caaaaagcta
    10321 caagcacagc aggcaaattt agctcaaacc gaaaaaaaaa agcctgagct aaaagcccct
    10381 caagttattg caaaaaaatc ggcaaatcag accgcttcat tgcaagagcg gtttatcagc
    10441 ttggcggaaa atcagcacaa agccgtactg caagtttcaa agaaaaccga atccgcccct
    10501 aaaagtgtgg aagatgaaga ttatcgctgg acgtggctca atcctgaatt agaaaatcaa
    10561 gcagacagtg ccaaaccttc tgatattaaa caagcgattt tgcaagaacg cacccctgaa
    10621 ttagtggcaa aaaccattca tttatcttgt cagcaggata aatggtgtga catcgccaac
    10681 tcactcaatt taggcggatt ttcccgtcag atcgccttaa atagctattt agcggaacaa
    10741 aatgaacagc acctcgcatt ggtgttaaag ccgaatatgg ctcacttaga taatccggaa
    10801 tcacgtaaaa atttagaagc tgcccttgcc gaaaaaggct attcatacga attgaccatt
    10861 ggtgacaata gcgaacagca aactccgctt gaaattcggc gagccatttt caaacaatta
    10921 actgaggcag caaaatcagc tctgcttaac gatgaaaaat taatgttact tcgcaacgct
    10981 tttgatgcac aaattgatga atcgaccatt cgagcggtgg cggaaactga gtaaaacaaa
    11041 tgaaagcatt agaattaatt gatttggtaa aaacctaccc aacaggtgta aaagccgtta
    11101 aaggcattaa tcttgtggtc gagcaaggtg atttctatgc cctacttggg cataacgggg
    11161 caggtaaatc tactactatt ggcattatca gctcactggt aaataaaacc agcggcagcg
    11221 tgaaagtgtt tggctatgat ttagacaccc aaaaagtcga attaaagcat caaatcggct
    11281 tagtgccaca agagtttaat ttcaatcagt ttgaaaaagt gattgatgtg ctagtgcaac
    11341 aagccgggtt ttacggcatt cctcgcagtg aagccttagt acgtgccgaa aagtggctga
    11401 aattgctcga cctatgggaa aaacgcaccc acctcacccg tgagctttct ggtggaatga
    11461 aacgtcgggt gatgattgcc cgagccttaa tgcacaatcc aaaactactg attttagatg
    11521 aacctaccgc aggggtggat attgaactcc gccgctcatt gtgggatttc ttgcgggatc
    11581 taaacaatca aggcacaact attattctca ctacccacta tttagaagag gcggaaaccc
    11641 tatgccgtca catcggcatt attcaacacg gcgaattaat tgaaaacacc tcaatgaaag
    11701 ccttgcttgc caaattggaa agtgaggtat ttgtgttaga tttgcaaaat tctgagcaaa
    11761 atcaaccgct tgtgattcaa aattacccgc ataaatggct tgatgaaaac acccttgaag
    11821 tgaatgtaaa acgccagcaa gggctaacca atctgttttc gcaaatttcc gcccaagggg
    11881 ctgaggtgct aagtatgcga aacaaatcaa accgcttgga agaactattt atcaatatgg
    11941 ctcagtaaaa tgaatttaat cggattttat accttagctt acaaagagtg ccgccgcatt
    12001 ttgcggattt ggcgacaaac tttagtgcca ccggtgatta ctaccacgct ctatttttta
    12061 atcttcggca cgctgattgg tgggcgaatc ggggaaatga atggcgtggg ttacacccaa
    12121 tttattgcac ccggcttaat tatgatgacc gccatcaccg cctcttatgt gaataccgcc
    12181 tcttcatttt tcttgagtaa atttacccga gattttgaag agatgctgat ttcgccactc
    12241 tctacccata atattatttg gggttatgtc accggcagta ttttgcgtgc aggtttagcc
    12301 gcaaccttag taatgtgcgt ggcaatggtg tttgtctcgt ttgaaatcta ctcggttgcg
    12361 attatcgtct taaccctgtt gctgacaatc attgcctttg cgttaggtgg cgtaattaat
    12421 gcggtgttcg cccgctcctt tgatgatgtg gggctgattc caacctttgt gcttactccc
    12481 ttaacttact taggcggcgt attttactct atttcattac tgcctgaatt ttggcaaacg
    12541 gtgtctaaat tcaacccgat cgtttatatg attaacggct taagatatgg ctttctcggt
    12601 atcagcgatg tgccgttagg ttatactttt gcagtgctta cttcactttg catcggcttg
    12661 tatattattg cctattcctt aattcaacga ggggttgggc tgcgttctta actaattgtg
    12721 aggttatgaa aatgaaagca ttaaaaaaac tagcaattac aatggcatta acgttatcag
    12781 gcacagcctt ttcacaagag attaccgtgt ttgctgccgc ttcaatgaca aatgtgttac
    12841 aagatattca gcaggatttt gaaaaagcct atccacagga taaaatcacg ttctccttcg
    12901 cttcatcatc ggtattagca aagcagattg agcaagatgc accggcagat atttttattt
    12961 ccgccgacca aaagtggatg gattatgttg ccgaaaaaca acctgagaaa gttaaacatc
    13021 gccgcaattt agtcagaaac gagttagtat taattgcacc taccgaaagt aacattgagc
    13081 caaaagcgat acaagcggtc gattttgcca aagaattagc aaacagctat ttatcggtgg
    13141 gagacccaaa ccacgttcct gccggcaaat atgccaaaaa agcccttagt cattaccaat
    13201 tatgggaaag tgttgaacct cgtttagcga gggcaaaaaa tgtacgagat gccctctctt
    13261 ttgtcgagcg aggcgaatcc ccacttggga ttgtctattc caccgatgct aaagtgtcga
    13321 ataaagtcaa agtggttgcc gttttcccac cggaaagcta cggcgaaatt gtttaccctg
    13381 ctgcggcagt cagtgaaaaa gcctctgccg ccacattttt agattttctc aaaaccccga
    13441 aagcaaaagc gaaatttgag gcagctgggt tttatcctgt aaattaaatt tactctctcc
    13501 ccttgtggga gagagacaga aaaatatgtc gttcagacat atttttcaga gagagggcaa
    13561 atagcgttta aaatattccc cctctccctg atttttcttc tgaacgaaga aaaatctatc
    13621 cctctcccac aaggggcgag ggtaagctta taaccaaata gttttagatt atgcttaatt
    13681 tcaccccaca agaactcaat gcgattgttc taagcctaaa agttgcctcc attgctgtag
    13741 tgcttgcatt accgcttgcc gtgtgggtgg cgtggctgct tgcccgcaaa cagttttggg
    13801 gaaaaaatct gctcaatggt attatgcatc tccctctggt gttaccgccg gttgtcattg
    13861 gctatttatt gcttatttcg atggcgaaac gaggggtaat cgggcaatat ttgtgggaat
    13921 ggtttcattt cagcttgagt ttttcgtgga aaggagcagt gcttgcctca atggtgatgg
    13981 cgtttccgct aatggtgcgt tctatccgtt tagcctttga ggcagttgat cctaaattgg
    14041 agcaagccgc cagaacgctt ggagcaatac cgttaaaagt gtttttcacg ctcactttac
    14101 cgctctcttt ttcaggcatt attgccggtg gcgtgctggg ttttgcccgc tctttggggg
    14161 agtttggggc aaccatcacc tttgtctcca acattcccaa ccaaacccaa accattcctg
    14221 ccgccctttt cacctttatt gaaacccccg atggcgaaat ggctgccgcc cgtttgtgct
    14281 tggtggcgat tctgatttca ctcattgccc tgttctgttc ggaatggctg gcagaaaggc
    14341 aaaaacgcta tgctcaaact taaacttctt cagcaactcg gcaatctgac gctcaatgtc
    14401 aatctcgatt tgccgaataa aggaatcacc gctattttcg ggcgttccgg tgccggtaaa
    14461 tccagcctga ttaatttaat tgcggggctt tcaaccgctc aagccggctt tatcgaatta
    14521 aacggtcagg tgctgtttga cagccaaaac ggcatcaatt taccacctga aaaacgcaac
    14581 attggctatg tgtttcaaga gcatcgcctt ttccctcatt attcggtgga aaaaaactta
    14641 aaatatggct acaagcggtc ggattctgcg gattttttgc aaattgtgga actgttaggc
    14701 attgcccctt tgcttaatcg ctatccggca agcctttccg gtggcgaaaa acaacgtgtg
    14761 gcgattgggc gggcgttatt aagctcaccg aatattttgc tgatggacga accattatct
    14821 gccctcgatc tccctcgtaa gcaagagcta ctggactatt tagataaatt atccgctcgg
    14881 cttgaaatac cgattctgta tgtcagccac agtttagagg aagtagttcg attggcagat
    14941 catcttgtgc tgttggaaaa cggcaaagta accgcatttg ataccctttc agcggtttgg
    15001 cacagctcgg cattttccgc ttggcagcca aatcatcagc gaattagcct gcttgaatta
    15061 ccggtggaag aaacacttcc ccactataat atgcttggct tgagcttgaa cgcacagcga
    15121 ttatgggtga accaactccc ccgccatcaa ataggcgaga agctaagggt aactattgcc
    15181 agcaaagagg tttcaatcag cttacaaaaa ccgactcaaa gctcaattcg taatgtgtta
    15241 aaaggcaatg ttcggaaaat agaaacgcat ttagatcggg ttgatctggc aattcaagtc
    15301 gaaaatcagc aaatttggag tacgatcagc ctttggtctt ttaatgaatt agcgattcaa
    15361 ccggagcaag aggtctatct tcaaatcaaa agcgtttctt tataggcttg caaatttacg
    15421 ctaaaatatg accgcttgtt aattcccaaa agagacatta tgccaatcgt taatcaatct
    15481 gccctcgtgc cttatagtgc cgagcaaatg tatcaacttg ttaatcagta tgaaaaatac
    15541 cccgaatttt tatccggctg cgtgagtacc aaaaccctta gcaccggcga aaatgaatta
    15601 aaggcagagt tacacattca gaaattaggc attagccaaa ctttcagtac ccacaataaa
    15661 atgacgccgc cctataaaat cgagatggca ttaattgaag ggcctttccg ccatttacac
    15721 ggagcttgga cattcacccc ctttgatgag caaagttgca aaattgccct gcaattaaat
    15781 tttgaatttt ccagcccaat tgtagcaatg gtgttcggca aggtgttcaa cgaattaact
    15841 ttaaaaatgg taaatgcttt caaacaacgt gcaaaagagg tgtatggtgt ctgaacaacg
    15901 tcaaaatcca gaagaacaaa aaagtgaaga acagaaaatt aatgttgagg tgctttatgc
    15961 ttaccctgat aaatattttc tgaaaaaact gcaagtaccg caaaacgcga ccattcaaac
    16021 tattattttg ctctccggcg tgttggaaaa atataccgaa attgacttgc gtgaaaacaa
    16081 agtcggtatt tttagccgcc cggcaaaact gaatgaaacg gtggaagaag gcgacagaat
    16141 tgagatctat cgcccgttaa ttgctgatcc gaaagaaatc agacgcaaaa gagcggctga
    16201 gcaagcggag aagaaatagc tttctcctat tcaatctagg ggtgaataat tattcgccct
    16261 atctattttc tccctcgcca ttcctgctat
//
LOCUS       DANLUA010000036        16223 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLUA010000036 DANLUA010000000
VERSION     DANLUA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16223
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..285
                     /locus_tag="PXB44_000869"
     CDS             <1..285
                     /locus_tag="PXB44_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281370.1"
                     /translation="AYPNLDEWYSASDKERREFIAKHFAEETVRSDRNTPYDYAMCTG
                     RMFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQNCAKYIQ"
     gene            complement(321..1073)
                     /locus_tag="PXB44_000870"
     CDS             complement(321..1073)
                     /locus_tag="PXB44_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL5281371.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1112..2359)
                     /gene="clpX"
                     /locus_tag="PXB44_000871"
     CDS             complement(1112..2359)
                     /gene="clpX"
                     /locus_tag="PXB44_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL5281372.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2359..2952)
                     /gene="clpP"
                     /locus_tag="PXB44_000872"
     CDS             complement(2359..2952)
                     /gene="clpP"
                     /locus_tag="PXB44_000872"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL5281373.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3064..3447
                     /locus_tag="PXB44_000873"
     CDS             3064..3447
                     /locus_tag="PXB44_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL5281374.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3551..3627)
                     /locus_tag="PXB44_000874"
     tRNA            complement(3551..3627)
                     /locus_tag="PXB44_000874"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3591..3593),aa:Asp,seq:gtc)
     gene            complement(3716..4471)
                     /locus_tag="PXB44_000875"
     CDS             complement(3716..4471)
                     /locus_tag="PXB44_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5281375.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4531..5526)
                     /gene="trpD"
                     /locus_tag="PXB44_000876"
     CDS             complement(4531..5526)
                     /gene="trpD"
                     /locus_tag="PXB44_000876"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL5281376.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5544..5930)
                     /locus_tag="PXB44_000877"
     CDS             complement(5544..5930)
                     /locus_tag="PXB44_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL5281377.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(5923..6513)
                     /locus_tag="PXB44_000878"
     CDS             complement(5923..6513)
                     /locus_tag="PXB44_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL5281378.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6523..8079)
                     /gene="trpE"
                     /locus_tag="PXB44_000879"
     CDS             complement(6523..8079)
                     /gene="trpE"
                     /locus_tag="PXB44_000879"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL5281379.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8221..8838
                     /locus_tag="PXB44_000880"
     CDS             8221..8838
                     /locus_tag="PXB44_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL5281380.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            8984..10051
                     /gene="rluB"
                     /locus_tag="PXB44_000881"
     CDS             8984..10051
                     /gene="rluB"
                     /locus_tag="PXB44_000881"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL5281381.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10117..11889
                     /locus_tag="PXB44_000882"
     CDS             10117..11889
                     /locus_tag="PXB44_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL5281382.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(11979..16130)
                     /locus_tag="PXB44_000883"
     CDS             complement(11979..16130)
                     /locus_tag="PXB44_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5281383.1"
                     /translation="MNKIYRIVWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLS
                     TLSLALSAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKV
                     STALGYNTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFI
                     GDHAGLNHSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKL
                     AGPTTNVTSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAY
                     GAEANATAIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGT
                     NAGATSDYASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESS
                     NASAFNATAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATV
                     SIGAEGKERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTE
                     NGNITFPTYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERH
                     LGSTVKIKGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGI
                     TIANGVADKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSIS
                     DDGIQRANYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADG
                     MNATSIGYNANANATNALAVGSAANANADTSTAIGTASTATATRATALGSKSEATGEN
                     STAVGYEASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAI
                     ALGNSANAAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKS
                     ATLNGNTYTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEI
                     DGLNTKVNELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANT
                     LTVKLAKDIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDIS
                     PISADAVNGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNV
                     GSAITALNDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNG
                     SLTVKLAKDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAA
                     GTKVTNVKAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATA
                     GLPQAYLPGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIG
                     MGYQW"
BASE COUNT         4468 a         3301 c         3471 g         4983 t
ORIGIN      
        1 gcttatccta atttagatga gtggtactca gcttcagata aagagcgaag agaatttatc
       61 gcaaaacatt ttgcagaaga aacagtaaga agtgatagaa atacaccgta tgattatgct
      121 atgtgcactg gtagaatgtt tgctaaacaa ctgactcgag atgaagtcaa agactaccct
      181 ttggcaaaca ctaagccgca atatttaacc cgtgatagac ggattgagat tatgtctaat
      241 ttacctcgtg aaatattaca aaattgtgct aaatacatac agtaatttta tgagtaacac
      301 cactttggaa ttaagtggtg ttattttaat tttaataatg tctctaaaat catctccaac
      361 ataaactcaa taaagccggt agaatcattt tggctgtctg cgagagaaag catttgataa
      421 taacgttgtt gattttcgta aaccattgtt tcaatcggca accaagcgaa aagtgggttc
      481 cattgtgcta aaaggacatt ttgccataat cgacccattc tgccgtttcc atcactaaag
      541 gggtgaatca tttctatttc atagtgcata accgagcttt taatgagtgc aggtgtatca
      601 tcttgtttag cccaagagaa caactcatta atcaaattct ctacgaattg aggacgagcc
      661 cccatatgca caagttgccc actgaaatta taaatgccga catcttttga acgaaaagcc
      721 cctgcgtcat ttactaagtc agcagtaaga aacttatgtg ctttgagaaa atctttaatc
      781 gagtaaggat tatagtgcat catttcatcg taagcgtcat aggcattttt gacttcacga
      841 atatctttgg ggtgtccgag aacctttttg cccttaatga ttgcggtaac ttgctcaatc
      901 gttaatgaat ttgcctcaat agctaaagag gcttgaattg atctcaagcg gttatttttt
      961 ctaagatgta aattacgctc aaaatccaat tgtagcgttc ccaatttatg agaaatttct
     1021 acaaccagat tcagcatttt agtggtaact gtaaaaggtg gtttataagc cattttgcaa
     1081 taatcctaaa aaattaaacc gcttgtcatt gttactccac cttcggctta tcgccgtttt
     1141 caacacagcc tttgccgatg gtgacttttt tcgcattgag ggttgggaga tcgtacattg
     1201 tgtcaagtaa taagttctcg acaatagaac gtaacccacg agccccggtt ttgcggctaa
     1261 tcgctttttg ggcaattgaa attaatgcat ctttggtgaa gtgcaattca acgccttcca
     1321 tttcgaataa ggcttgatat tgcttgatga tcgcattttt cggctcggtt aagatttgaa
     1381 tcagagcttc ttcgtccaat tcttgcagcg gagtcaccac cggtaaacgc ccgattaatt
     1441 caggaattaa gccgaatttg actaaatctt ccggttcaac ttgtttgaat agctcggtta
     1501 aatcttggcg atctttatct tttttcaatg ccgctgcaaa accgatacca ccttgagtgt
     1561 tggtacgggc ttccacgatt ttatctaaac cggcaaaagc accgccgcaa ataaagagaa
     1621 ttttggaggt gtccaccgga atggtttcgc ctttcgggtg tttgcgaccg ccttgtgggt
     1681 taatgttcgc caccgtgcct tcaagtagtt ttaataaggc ttgttgaaca ccttcgcccg
     1741 atacatcacg ggtaatggat gcactttccg atttacgggt gattttgtcg atttcatcaa
     1801 taaaaacaat gcctcgttcc gcttgttcgg cttcaaaatc gcatttcatt aataattttt
     1861 gaatcacgtt ttcgacatct tctcccacat agcctgcttg ggtaagggta gtagcatcag
     1921 ctaccgcaaa aggtacgttt aaacggcggg caagggtttc tgccaacaag gttttaccgc
     1981 taccggtcgg cccgattaat aaaatattac ttttacctaa ctctacgcca ttagtggctt
     2041 gatggtttga caatgcactt cttaagcgtt tgtagtggtt ataaacggcc accgaaagca
     2101 cttttttcgc gtgttcttga ccgatgacat aatcatctaa atgagcgtgt aattcgtgag
     2161 gtgtcggcac attggtgaaa aaactgcctt catcgtcttg gttttcatca attaacggct
     2221 cttcttcacc gtgtaataag gcgtaggatt cctcgataca ttcattacaa atatagcctt
     2281 ccgtgccttc gactaattga tcgacttcgg tacgtttttt accgcaaaaa ctacagtggg
     2341 gttctttttc aaatgccatt acgccacctc actgcgagaa gttaatactt tgtcgattaa
     2401 gccatactca gcggcttctt ccgcagacat aaaattatca cgctcggtat cggctaacac
     2461 tttttcatag ctctgaccgc tgtgttctgc catacggcgg gtaagggtag ctttcaagcg
     2521 taaaatttct tccgcttgaa tttgaatatc ggttgcttga cctctcgctc cacctaaagg
     2581 ttggtgaatc ataatacgag cgtgcggcag agcaaagcgt ttaccttttg ccccggctga
     2641 aagcaaaaat gcccccattg aagccgcttg ccctgtgcaa agggttgcta catcgggttt
     2701 gatgaagttc attgtatcgt aaattgccaa gcctgctgtt accacgccac ccggtgagtt
     2761 aatgtataaa tagatgtctt tttccggatc ttcggcttct aaaaacagaa gttgtgccac
     2821 aatcaggttt gccatattgt cttccacctg accgtttaaa aaaataatac gttcttttaa
     2881 taaacgtgaa taaatgtcgt aagaacgttc gccttttgag gtctgttcca ccaccattgg
     2941 gactaatgcc attttgtttt cctctgtcgt aatacaaaat ctcgctattc tagcaaattt
     3001 tcagaaaatc attaacaaca agcggtcaga ttgttaaaat cttttgcaaa tgatgaggaa
     3061 attatgacat taacagaaat ttaccaaaaa tttgaacgtt gtaaatcgtg ggaagaaaga
     3121 taccgcttgt taattcagct aagtcgcttg ttaccaaaac caagtgagca agagttggca
     3181 caaatagcgg aaattgaggg ctgtgaaagc cgtttatggt ttgaatttca agctgagcca
     3241 cgaaaagtag tggcatatag cgatgcccgt ttaatgcagg ggattttatt tattgtgagt
     3301 gtagctttaa ctgaaaaaac ggcagaagag ctgaaaggct ttgacttaaa aacgatgttt
     3361 gatgagctaa aaatttcaca aaatttaacc agcacaaggt taaatgggtt gggtcagtta
     3421 cagaaacata taatgatgaa tttgtaattc accctcgccc cttgtgggag agggtgaata
     3481 aaatccgcag gattttattc gggagagggg aatataaaaa acctctgatt tctcagaggc
     3541 ttaaatttgt tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
     3601 tattctaacc agctgaacta ccgctccata ctggtgctaa aataatagtg gtttcctcaa
     3661 cattgagcaa atagtttttg caaaatagtg tttaattggt tgtaaaataa ccactttatt
     3721 tctgcttgat cgccttgata aacagcaacg gcggacggtg ctgtaaatct ttaaattcat
     3781 tgtgccaccg aggttggcct gccagcattg gttcttccat ttgcacaatc tcaaacccgc
     3841 cttgaataag gttattcaca atggttgcca ttgtgcggtg gtaggtttta aagggttgtt
     3901 gaaaccaact tctgtcccgt tcgccctctt cccgataata gttcaagcgg taagcaactt
     3961 gttgtttttc ttggtttttc tcccaacgat agccttctcg gtaacaggtg acaatcgggt
     4021 gttcttgcga gaaaatcagc gtgccacaag cagtcatttt ttgcgaaatt tttgcaagta
     4081 gttcggaaaa atcagcaata taatgaaacg caaaagagct ggttactaca tcgaaatcgc
     4141 cctcgctgat ctcatttaat cgctccatcg gcaggcagta aagggaaaaa tcgttgcctt
     4201 tttgccaatg agcagaaaga tcgctttctg cctgttgtag cattaattca gataaatcca
     4261 accccaccac tttttctgca ccgagcgtta aataatgggc taaatgaact cctgtgccac
     4321 aacctaaatc cagcactttt ttgccggtta agtctggcag tagagagaac attgttggct
     4381 tttccaccac ttcattcata cttattggat tttgtcgaag ttgttggtaa cgctcaaaga
     4441 aaatcggagt gtcgtaaatg ctttttttca cttaatagcc tttaaacaaa tataaaataa
     4501 ggcgggttaa aacccgccac tacgatatga ttagtaagtc gataattttc gcaaagtttc
     4561 taacgctttt ccactctcaa gcgtttcttt tactttttgc acattggctt ttaaatcatc
     4621 ttctccaaag gttttcatta ataatgcgac atttgctgcc accgcatcaa tatgagccag
     4681 tttacctttt ccttgtaaaa tagcggttac tttttcggca ttttctgcag gttcaccgcc
     4741 tttgagatct tctaacgaat ggcgttcaat gccgaaatct tccggcgtta aggtgtaata
     4801 gtcgatctgt ccgttttgaa tttccgcaac ctgcgtttcg ccgtgaacgg caacttcatc
     4861 taaacctgca ccgtatacca caatagtgtg ttcgtaacct aaggctttta cggtttcagc
     4921 atagactttc actaactcag gggagtacac accgagtaat tggcgtttag cgtgagccgg
     4981 gtttaccaac ggccctaaaa tattgaaaat cgtgcgggtt tttaaggctt ggcgaacagg
     5041 ggcggcatat ttgaatccgc tgtggtaggt tggggcaaac aaaaagcaga tgttggtttc
     5101 atctaaagca aggcgtgccg tttcaggtga aacgtcaaca ttaataccta aatggcttaa
     5161 tacatcgctt gagccggttt tacttgatac gctacggttg ccgtgtttgg caattttata
     5221 gcccattgtg gcggcaacaa tcgcacttgt ggtagaaatg ttgatggtat tatgcccatc
     5281 gccacctgtg ccgacaatat ccgcaaaagg gtaatcaggc gttgagaacg gattggcatt
     5341 ttcaagtgcg gcttttgcag cacctgcaat ttcatcaacg gtttcgccac gcagttttag
     5401 agcgattaag gcagcagcaa gttgttcatt tgatacattg cctttaataa cttcactaaa
     5461 aaaacggtga gattccgctt gggctaaggc ttggttggcg aataatttat ctaaaagtgc
     5521 ttgcatagtc tcttcctttt taattagcga tgtgcctgat attccaaatc ggttgcctcg
     5581 ttgccggtca ttccacgaaa gccccagcaa tgctctggct gttctttaat ggtgatttca
     5641 acgtccattg gcacaatgcc gactttggtg tgtaaggcac taaatagtcc tttaattaag
     5701 cgttttttga gctgttcgct ccgcccttgc atcaggttta tttcaatcac aataaaatct
     5761 tggctacggt taattggggg atagaaattt tccgcatcca ataagtcaaa acgcaaagcg
     5821 tggcgttgtt ttggtacgcc aaggctcatt tgcatacagt caaaaatcac gtctgcaatc
     5881 agggctctgc ggtctgctaa ggtttgtttt aatccatata cactaatcat ttttttctcc
     5941 taataaccat tccaccgact gcttaagtaa ttttgtgcct tgcacggtta aaatggattc
     6001 cgggtggaac tggaaaccgc aaatcggtaa ggttttgtgg cgaattgcca tcacaatatg
     6061 ttcaaatttc gcattaacga taaattctgc cggtaattca tcgcccatta atgagtgata
     6121 acgagcgaca agcattggat tgttaatgcc tgaaaacatc gcttggttat cgtgttcaat
     6181 acgagaaact ttgccgtgca atactgtgcc ggtatgcact actttgccgc caaaggcctc
     6241 aattagagct tgatgtccta aacaaatgcc aatcatcggt actttgtctt tcagacggcg
     6301 aataatttcc aataaattgc ctgcctcagc cggattaccc gggccggggg aaagggcaac
     6361 caaacaattt tcggtggttt cagctttggc taaaaaatcc tcaagcggat aatcattgcg
     6421 gaaaatcgtt acctgatgcc ctaattcacg aaattgatcg accaagttat aggtaaaaga
     6481 gtcgaaatta tcaataaaaa gaatatttgc catagtttgt ccttatgctt ttgcattcac
     6541 ctgcccaatc gcttttaaca ccgcgcgtgc tttatggcgt gtttcatctg cctctaattg
     6601 tggatcggaa tccagcacct caccacaacc agcttgcaca taggcaatgc cattttgaac
     6661 aaaggcggaa cggatcacga tacaggtatc taaattgccg tctgaggtta aatagcccac
     6721 cgcaccgccg tagctatggc gtttttgttg ctcgacttgg tagatcagtt gcattgcctt
     6781 aattttcgga gcaccagtta gcgttcccat attcatacaa gcttgataag cgtgcagagc
     6841 gtcaagatct tttctaagtg tgccgatcac acgagaaact aagtgcataa tttgcgaata
     6901 gcggtcaatt tgcattaaat caaccacacg gcgagtgccg gattcgctca cacgagccac
     6961 atcattgcgg gcaagatcga ctaacattaa atgttcggca agctcttttt ggtctaaacg
     7021 cagttctaat tccaaacggg aatctaattc aggatcgata ttaccttgtt catcaaaacc
     7081 gcgtgggcga gagccggcaa tcgggtaaat ttccaactgg cggttggctt gcgaatattt
     7141 caacgcactt tcaggcgacg ccccaaacag agtgaaattt tcgccctgca tatagaacat
     7201 atacgggctt ggattgttga ttttgagctg tttatagctg gcaagagtat tcgggcaagg
     7261 caagctgaaa cggcgagagg gcacaatttg gaacacatca ccgatattaa tatggtgttt
     7321 gagtttgcgg ataatcgctt tgaaatcttc atcttcaata ttggcggttg cctcactcgt
     7381 tgcggtttgg ttcgctagag gcagctcaat atgtgacaga tttgcaagtt tttgcgtaaa
     7441 ttcgaccgct tgttggttca aattggtttg ttcattcggt tcaaaacaga aggtgtttag
     7501 caccgcagtt tcggtttggt ggtcgattgt aaccagttgc tctgctaaat agaaagtgta
     7561 atctgggcaa cttaagccat catcttgcag gctgatattt tccatcggaa taaaatttgc
     7621 caccaaatcg tagctaaata atccgcccaa atagataggc gatgtggaat cagcaaacag
     7681 ttggctcacc gttcttagcc cgtcaaacac ggtggtggtt ttgagcttgc tgtcttcatc
     7741 taactggttg ctgatggttg ggaaaatcgc ttgcaagcgg tcgtttttgt ctaaaaattt
     7801 gcaagtcgga gcagggttta acaggctcaa tgcgtttgca atcaaaggta aaaccgcttc
     7861 tccgtttggg gttaaagcgt ggaaagtcac ggtttgatct tcacagaaaa tatgcactgc
     7921 cgatttcgca aacagcaaac tttttacact gtttttactt tgaatttccg ctgaatctaa
     7981 taatagcgta tgtgggcgtt cgccgcataa tgcgtaataa acacgggtcg tatcggcaaa
     8041 atagggtaat tctgtttgag taatcgttaa ttgaggcata gcaaattcct attcaatttt
     8101 tcgtactatt aaactagtac attaaaaaga agtcaagcaa ttattccaga taattatgcg
     8161 agcttgggcg aacttggtgt ataattagcc caatttttag aaagtcgaag gcttgagact
     8221 atgacacaat ttttttatat ccaccccgac aacccacagc aacgcttaat taaccaagcg
     8281 gtagagatta ttcgaaacgg tggcgtaatt gtttacccta ccgattcagg ctatgctctc
     8341 ggttgtgcaa tgggcgataa gcacgcaatg gatagaattg tagcaattcg taaattgccg
     8401 gaaaatcaca attttacctt agtttgtagt gatttatccg agctttctat ctacgcgttg
     8461 gtcaataatc aagcctatcg cttaattaag aataataccc cgaatcctta tacttttatt
     8521 ctgccggcaa ctaaggaact tcctcgcagg ctgatgacaa agcgtaaaac agtggggatt
     8581 cgtgtgccgg ataatgcgat cgcattggcg ttaatttccg ccttaggtga gccgattctt
     8641 tcttgctcgt taatgttgcc ggagacggaa atgaccgagt cagaccctga tgaaattcgg
     8701 gatcatttgc aaaatcgggt ggatttaatt atccacggtg gttatttagg gcagcagcct
     8761 acaacggtag ttgacttaac cgatgacgtg ccggttatta ttcgagaagg atcgggcgat
     8821 ccaacatcgt ttaattaaat tgatgttgta tgattaatat atagaattat gcgtaggggg
     8881 gctgtgccag tccgtattaa tatcgttatt tggacaggca caggacctgt ccctacaatg
     8941 aaaatcaaat tcagacgctt gtgaaagcga caaaaggaac cttatgacaa cttttcaaaa
     9001 aaaatcaact gcgactttcc gtgcagacaa acgtggtgaa agccgtccct catttaaaaa
     9061 agaagagcaa aaatctgaac gcaaatttgg taaaacgtca gaaaaaccac cacgtccaac
     9121 ttctaagcca ttaacgcaaa gaattgcaaa aaaagtggaa aaaacgaccg ctagtgaagt
     9181 ggcatctcaa gagaaaaaag ccaaagtagt aggtgaaaag ctacaaaaaa tcttagcgcg
     9241 agcagggcaa ggttctcgcc gtgaattaga agagattatc gctgctggac gagtgagcgt
     9301 ggacggtaaa atggctactc ttggtgatcg tgtgcaggtt agctcatcga ctaaaatccg
     9361 cattgacgga catttagtta atttaatgcc ggcacaaaaa gagatctgcc gtgtattaat
     9421 gtattacaaa ccggaagggg aactttgcac ccgttccgat ccggaaggac gatccaccgt
     9481 atttgaccgc ttgcctcgtt taagtggcgc tcgttggatt gcggtcgggc ggttagatat
     9541 taacacctca ggcttattat tgttcactac cgatggcgaa ttagccaacc gtttaatgca
     9601 cccaagccgt gaagtcgaac gtgaatattc ggtgcgtgtg ttcggcaaca ttgacgatgc
     9661 gatgttacaa cgcttacgca aaggcgttca gttggaagac ggaccggcaa actttaaaca
     9721 aattaaggtt gtgggtggca caggcttgaa ccaatggttt gatgtgactt taaccgaagg
     9781 gcgtaaccgt gaggttcgcc gcctgtggga atcgcaaggg gtagaagtca gccgtttgat
     9841 tcgtatccgt tacggcaata tcaaattaga taaaggctta cctcgtggcg gctgggaaga
     9901 aatgggctta gaacaagtca attacttgcg tgagctggta gggttaccgc cggagactga
     9961 aactaaagtg gaagtggggg taaaccgccg ccgaaccaat atccgtcaaa ttcgtaaagc
    10021 ggtaaaacaa catcaaaaat atcgtggcta aacaccatct ttggggctgc attagttctg
    10081 ctaatgcagc tattttcttt caaaaaagga gcatttatgt cttgggatat tgttgcaaca
    10141 ttggcggtca ctgggattgc cgtattttta tttgcaactg aaaaaatgcg aatggacgta
    10201 gttgcgattt tagtgctgtg ttcattagta ttattagggc aggttgatac caaatctgct
    10261 ctgagtggat tttcaaactc agctactgta accgtaactg caatgtttgt attagctgct
    10321 ggtttacaaa atagtggtgc tttagatagt gtaggcagtt tacttgctaa ggtaaaatct
    10381 ccttggttat tcttgcttgt actctgtggc gtaaatgctg ctgtttctcc ttttgtgaat
    10441 aatacggcag tggtcgcagt attaattccg attgtgattg ccgcagcaca gaatataaaa
    10501 atggctcctt ccaaagccct tattccgtta tcattttctt ctcaaatggc aggtgtttgt
    10561 acactgatcg gcacttccac taatctatta gttaatgcaa tcgctcaaaa gcaagggcat
    10621 tccggtttcg gtatgtttga atttgcccct ttggggatta tttttttagt ggttggggtt
    10681 gcctatttat tagtgaccag ccgtttctta ttacccgaat cacatttgca acttgaagat
    10741 ggtgaggggt ttggaaaata tgtttctgaa cttaaagtaa ataaagactc tgtattaatt
    10801 ggcaaaagta catttgaatc aggattaaat gaagaattta atctatttac tattggtgtt
    10861 ttgcgtgatg gggaaagatt atcgacaccg agtcatcaag tattgcaaaa acatgatatt
    10921 ttattgttgc ggggtgaatc ggaaaattta gcaaaagtac gagaaaaata tggattgcac
    10981 cacgttgtgt atggacgccg tgagaatgat gaagataatc tggatgaaga cttgatggtg
    11041 gctgaagtga tgatttctcc tatttctcgt tggataggag ggactattcc tattttacaa
    11101 cagcgttgga ataaaaatgc aacggtgtta ggcattcaac gccaaagtaa agtaattcgt
    11161 gagcgtttac gcaccactgc ttttaaaatg ggggatattt tattactcac attaccaaaa
    11221 gaggatatgg agacgttacg ccaagataaa gactttattg tgttatcttc tgatttagtc
    11281 aaaacggagg aatcgtggaa aagtaaattt gctttagggg taatggtttt ggtggtggca
    11341 acagcagcct taggctgggt tcctatttca gtgagtgcct taattggtgc agttgcgatg
    11401 tgtgttgctg gttgtttaac ggctgaagaa gcctatcaat ctattgactg gaaaatcatt
    11461 ttagtcttag ctggattact gccattaggt gaagcgatgg caaatagtgg ggcggcgcaa
    11521 tttattgttg ataacacgct tggtaaagta ggggagttcg ggcctttagt ggtgttagct
    11581 gtattatatt tactgacaat ggtgttaacc gaatttatga gtaatgcagg gactgccgtg
    11641 ttgcttacgc ctattgcggt ttctaccgct aaaatgttag gggtagatgc ttccccattc
    11701 attattgccg taatgtttgc ggctgcgacc agctttatga cccctgttgg ctatcaaacc
    11761 aacacgatgg tatacggtgc aggtggctat aaattcacag attttattaa aattggttta
    11821 ccgctaaatg taatctattg gattttaggg atagtattaa ttccgatttt cttccctttt
    11881 aatccataat tgatagataa ttatagaaaa aaggtttaca gatcagaaac tctgtaaacc
    11941 ttttttattc agcaataaat caattttcta ttggaaaatt accattgata acccatgcca
    12001 atactaccac caaagtcgcc tctggtattg ctgttaccag ttaatttata gataaccttc
    12061 ccgttatctg aaatccgaga ataacctact gcgattgcag cttcattacg gtatgtatct
    12121 ccagccgttg ctaacatact tttacctggt agatatgctt gcggtaaacc tgcggttgca
    12181 gttgcaccag caataccagc gcgtaaacgt ttatcaactt tgtttacttt tgaatcaata
    12241 ttattgatct tattattgat atttttttct gttgctttta attggctaaa gttaactgca
    12301 tcagtatctt cagttcctgc ttttacgtta gtcacttttg ttcctgctgc attaatacct
    12361 gttttagtta tactcggtcc ccctacaata gttaatccat tagtatcaat ggttgtatca
    12421 ccagtcttaa cagtatcggc tacagcaatg ttagctgtta ctgtttgagc tgtgacagaa
    12481 ttaactttaa tatctttagc taatttaacc gttaatgaac cattaccatt tgaaataacc
    12541 ccgatattat tatcagttag ttgtgattcg ttagataccc caccttttac tgtaacagta
    12601 gaacctaatt tacgttgaga tggcgtgccg gtatcacctg caaatgttag tgggctggtt
    12661 actgcatcat ttaacgcagt aattgcagag cctacattat tgaccgtttt actaacttca
    12721 tttgtaggag cgccatcaac cacagtatag cttggggctg aaacagcacc atttgcattt
    12781 acacttgaat taccacctaa agcggtagca atagtatttg ccgtatcgta aagttggcta
    12841 ccattaactg catctgctga gataggtgaa atatcaccat cagctacatt agttattttt
    12901 aatttgccag catcgacacc tgtcattgtg acacttgggc cattattaat ggttaagccg
    12961 ttgttattta gttttgaatt accaacagct acggagccgg caggagttaa atcaatgtct
    13021 ttcgctaatt taactgttaa tgtgttagca tctgttgcca ctacaccgat attattatca
    13081 gtcaatatgg ttgcatcggc cccaccttta acattcagag aagtacctaa tttacgaaca
    13141 atttcagtac cagcatcatc tacaaaagta agtgctccat tacttaactc attcactttc
    13201 gtgtttaaac catcaatttc ggtataagca gcataaagtt gtgaaccgtt gatagcatcc
    13261 gttgatgagt ccaaaatacg acctgctgca acattcgtta aagtacgttc attgcctaca
    13321 gaaccaatac ttacagttga gcttggcgta gtacctgcaa atgtgtatgt attgccattt
    13381 agcgttgcac tttttgttgc aactgtggct actgtttcag agttagtacc taatgcaaca
    13441 gaaccttggt gtgttgcttt tgcattttgg ccaagagcca ctgcagcagt tgttgttgct
    13501 tgtgatgaaa cacctaatgc aatagaagct acaccggctg catttgcact attacctaaa
    13561 gcaatcgcag agctacctgc agcattggca tttctaccca cagaagttga ccatattcca
    13621 cctgcattag cagagttacc taaagcggtt gactgtgtac ccgaagcatt tgcgttatag
    13681 cctgcagcaa gagaatctgc accgattgaa cttgcttcat aacctacagc ggttgaattt
    13741 tcaccggtag cttcagattt agagcctaat gcagttgcac gggtagctgt cgctgttgaa
    13801 gctgtaccaa tagctgttga ggtatcagca tttgcattgg ctgcagaacc aaccgcgagg
    13861 gcatttgttg catttgcatt cgcattgtag cctatagaag tggcattcat accatcagct
    13921 gttgcgcgag gaccaactgc agttgtacgt tcaccgtttg cttctgcttc agagcctaat
    13981 gcggttgcca gctcaccatt agcacttgtt gcaacgccga tagccattgg attaacaccg
    14041 ctagagcctg aattatcata gttagcacgt tgaatgccat catctgaaat actaacataa
    14101 tgagttgcaa atttgctgat agcttgtttg agttgactga cattgaccgc atcagtatct
    14161 acatcgcctg cggcaacatt ggcgatttta ttaccgccat tatctaagcc agatttagta
    14221 agacttaccg gtttgtctgc aacgccgtta gcgatggtga tacctgtatt gttaacaacg
    14281 gtatcacctg ttgttaatga accattagcg cctaagttaa ctttttctgc taattttatg
    14341 gttaaagtac tattgccatc agctacaaca ccaatattat tctcggttaa tatgcctgta
    14401 gatccgcctt tgatcttgac tgttgaacct agatggcgct caaaattagt tcctgtatca
    14461 ccggcaaagg ttaacggact gatcaccgct gtgtttaatg cgcttagtgc tgcagaaaca
    14521 gtagtgtatc tcccttgttt gccttcttta tctgcatctg gtgtgccatt cactaaagcg
    14581 taggttggga aagtgatatt tccattttca gtaacagctg ccccaccacc taagatattt
    14641 gcggttgttg cagcaacatt acctatagct tgttgtgtta aatataattg tgaaccgttt
    14701 atggtatctg tagagcttaa agatatacgt cctgcagcaa cattggtgat agttctctct
    14761 ttgccttccg ccccaatact tactgtagca attggatttg ttccagcaaa gtcactatat
    14821 gtaataccat ttacagttgc atttgtagtt ggtatggcta tagctgtaac tgagttgcta
    14881 cctagcgcta tcgaatcggt atgttcagca gtggcatttc gacctatagc ggttgcatta
    14941 aaggcggaag catttgatga ttctccgatt gcaattgtgc gttgatttgt tgctttagcg
    15001 cttaccccta gtgcaacaga agccgcgcct gatgcagtag cacgattgcc taaagcggtt
    15061 gcagaacctc cacttgcatt ggcttcacga ccaaatgcag aagcataatc gctggttgca
    15121 cccgcattag taccaacagc agtagttaaa tttgctgttg catttgcata ataacctaat
    15181 gaggttgcac cgtggcctga agtcttcgct tcataaccaa ttgctgttga gttatcgcca
    15241 tttgcttgag attttgcacc aattgctgtg gagtgaatag ctgttgcgtt agcttcagca
    15301 ccataggcgg agctaacaat accagttgca ttggcatgac tacctacagc agttgctttt
    15361 cctattaccg tttctcctgt tgctgctgta gttttgtaat tattaacacc atttgcaaga
    15421 gcggaattac cgatggcgat attatcatca ctggttacat ttgtagtagg tcctgctagt
    15481 tttgcattag cataagtacc aattgcaata ttgtggctac cactagtacc gtctccagca
    15541 gtattaccaa tagaaatcgt attgtttcct gttacattag agctagccga ataaccaata
    15601 ccaatattgt attctgtgct gttagagtga tttaatccgg catgatcgcc aataaaaact
    15661 tcacgtaagc ccattgcggt tgcattgtac cctactgcta cggaacgttt atcggcagtg
    15721 gcttgcccac caacagctgt tgaagcaaaa ctagcgttag caaagcctcc taatgcagtt
    15781 gaaaaatcgg ctgtcgcggt tgtgttatac cctaatgctg tagaaacttt accgctattt
    15841 ccttctaagc ctgttattgt gcttactagt gttgagttaa ctttagatcc atcaccaatt
    15901 gcgatagatg tttgtgggtt attatctggt atggtcccat aggttggtga ataggttgca
    15961 gcacttgcaa aacctgcaga aagcgcaaga cttagtgtgc tgagtttaat aatattagtg
    16021 gttagtgatg tatttgttgc attagattta ttagcacttc ttgataattc agaagcaaca
    16081 atccaagttc gttttgcatg gttccaaaca atgcgataaa ttttattcat agaactactc
    16141 ctgttcttat tttttaataa ttttaatgat ttgagtattg cctagccaaa tatatacaat
    16201 aggctgaaaa attatatagg tga
//
LOCUS       DANLUA010000037        16210 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLUA010000037 DANLUA010000000
VERSION     DANLUA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16210
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            183..2405
                     /gene="glgB"
                     /locus_tag="PXB44_000884"
     CDS             183..2405
                     /gene="glgB"
                     /locus_tag="PXB44_000884"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL5281384.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2429..4441
                     /gene="glgX"
                     /locus_tag="PXB44_000885"
     CDS             2429..4441
                     /gene="glgX"
                     /locus_tag="PXB44_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL5281385.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4434..5747
                     /gene="glgC"
                     /locus_tag="PXB44_000886"
     CDS             4434..5747
                     /gene="glgC"
                     /locus_tag="PXB44_000886"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL5281386.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5870..7306
                     /gene="glgA"
                     /locus_tag="PXB44_000887"
     CDS             5870..7306
                     /gene="glgA"
                     /locus_tag="PXB44_000887"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL5281387.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7401..9901
                     /locus_tag="PXB44_000888"
                     /pseudo
     CDS             7401..9901
                     /locus_tag="PXB44_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(9983..10699)
                     /gene="trmJ"
                     /locus_tag="PXB44_000889"
     CDS             complement(9983..10699)
                     /gene="trmJ"
                     /locus_tag="PXB44_000889"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL5281388.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            10879..12417
                     /gene="pntA"
                     /locus_tag="PXB44_000890"
     CDS             10879..12417
                     /gene="pntA"
                     /locus_tag="PXB44_000890"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL5281389.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            12432..13877
                     /gene="pntB"
                     /locus_tag="PXB44_000891"
     CDS             12432..13877
                     /gene="pntB"
                     /locus_tag="PXB44_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL5281390.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(13944..14870)
                     /locus_tag="PXB44_000892"
     CDS             complement(13944..14870)
                     /locus_tag="PXB44_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5281391.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(14980..16095)
                     /gene="asd"
                     /locus_tag="PXB44_000893"
     CDS             complement(14980..16095)
                     /gene="asd"
                     /locus_tag="PXB44_000893"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL5281392.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         4657 a         3123 c         3695 g         4735 t
ORIGIN      
        1 gtttcaaaat gtgatctatg tcacaaaatt taaggtttga tgatttaaac taaagattaa
       61 aacgatttta ttttgtgatt tagctcaaaa aatggcgtaa atccacttct tttatttcta
      121 tttagtgata gagtgagaac tattgaaatt taagtattaa acgctttaag caaggagtgc
      181 cgatgcaaga ctatacttat tctgaaaaag atgctttttt tgttgagcga ctttcttcgg
      241 gttcttgtaa tgatcctttc tcctacctag gtattcaccc tagcaataac ggcgttatta
      301 ttagggtatt tcttccttct gccatagctt gccaagtgat cgatttaaac ggaaagcctg
      361 tgtcatcaat gctaaaaatt gatgatagag gcttttttat tgcagagctg attggtaaaa
      421 aaacagattt ttcttaccgc ttgttggtgg aataccctga ttcaacggtg gatatagaag
      481 acccttaccg ttttaaaacc catattcaag agcttgataa ttggttgttt gcggaaggaa
      541 aacaacttcg cccgtacgaa aaattaggcg cacatttagt cacacaaaat gaagttgggg
      601 gtgtgcattt tagcgtctgg gcgccgaacg ctcagcgggt ttccgtggtt ggggatttca
      661 accactggaa cggcttggta cattcaatgc gattccaccc cgattccggt atttgggata
      721 ttttcctgcc gaatgtgaaa aaaggggagt gctataaatt tgaagtgttg gatcgaaacg
      781 gcgtgctacg gttgaaatcc gacccttacg cctttgcctc ccaattccgc ccagaaaccg
      841 catctattgt ttccggcttg ccaagcattg tgccagccga gaaaacctgc cgtaatgcga
      901 atgctcccga taaaccgatt tccatttatg aagtgcattt aggctcttgg cgacgcaaca
      961 ttgaaaacaa ttattggctt agctatgaag agcttgccga tgaactcatt ccttatgtga
     1021 aagatatggg ctttacccat attgaattgc tgcctatttc cgaatttccg tttgatggct
     1081 cttggggtta tcaaaccacc ggtatttatt cgccaaccag ccgttttggt acgcctgagg
     1141 gcttgagtta ctttatccaa aaagctcacg ataaaggcat tggtgtgatt cttgactggg
     1201 tagtggggca ttttccggta gatgaacacg gcttggttta ttttgatgga acgcatttat
     1261 acgaacattc cgatccgaaa gagggctacc acaaagattg gaatacctta atttttagct
     1321 acggcagaaa tgaggtgagc aattatttgt ctggtaatgc actttattgg attgagcgtt
     1381 ttgggattga tgcgttgcgg gtggatgcgg tggcgtcaat gatttatcgt gattactcac
     1441 gttcagacgg cgagtggatt ccaaataaat atggcggacg tgagaactta gaggcaatcg
     1501 acttcttacg ccacaccaat aatattttga gagacgaaag ccaaggcggt gcgagcattg
     1561 cagaggagtc cacttctttt gccggagtgt ctcactcaac tgaaagaggc ggtttgggct
     1621 ttaattataa atggaatatg ggctggatga acgacacttt acgctatatg caaaaagatc
     1681 caatttaccg ccgttatcac cacaatttaa tgacttttgg tatgacctac caatacagcg
     1741 aacagtttgt gttaccgctt tcacacgatg aagttgttca cggcaaaggt tcattgctga
     1801 ataaaatgcc gggggattgc tggcaaaaat ttgcgaactt acgagcctat tatggctata
     1861 tgtggggcta tccgggcaaa aaattgctct ttatgggcaa cgagtttgct caaggtaaag
     1921 agtggaattt tgccgagagc ttagattggt atttactcgg ggaagaaggg ggcgaatggc
     1981 ataagggcgt attgggttgg gtaaaagccc ttaaccattt atacacccaa acccctgcac
     2041 tgtttgagca agattacaat cctgaaggtt ttgagtggtt agtggtggac gatcacgata
     2101 attcggtgtt tgcttttgag cgtaaagcca aaaatggcga ttcggtgatt gtggtgagta
     2161 atttcacccc gattgtgcgt gaaaattacc gttttggggt gaatgaattg gcagaatatc
     2221 aggaaattct caattctgat ttagcttgtt attgcggtag taatgtcagc aatgcaaacc
     2281 gatttgccag cgagttaatt gcatcgcacg gtaaagccta ctccttgagc attactttgc
     2341 caccgctatc gactatcttc ctcaaaagag ccgcagacaa aaaaacaaaa caaaacaaaa
     2401 cataacttaa agaaacataa aaaataatat gcactatttt agaggtaagc cttttccttt
     2461 aggcaataaa cttgatagac aagcgggtga atttggcaca aattttgcca ttgtttcgca
     2521 aaatgcaacc gctattgagt tatgtctttt tgatgaaaat caccacgaaa cccgtattcc
     2581 gatgttttct tccggagata cttggcacat ttttgtagca ggtgtacaag cagggacaaa
     2641 atatggctac agggtgaaag gcatacaaga tgaacaagcc ggtttgttat ttaatccgca
     2701 aaaattattg actgatcctt acgctaaagc gattgaaggg aagccggatt taagtagtgc
     2761 agaggcagcc gcttggtata aatggaacga tgaacgggat aatgcctctc ttgcacctaa
     2821 gtctatcgtg attgatgagc attttgattg ggaaaatgat agccagttat tcactccttg
     2881 ggcagagact attatttatg aaatgcaggt gaaaggtttt agtcagcgga actttcagat
     2941 tccaagcgac attcgaggca cttttgccgg tttggctcac cctgtttcga tagactattt
     3001 aaaaacctta ggaatcaccg cagtagaact tctacccgtt acttaccata ttgatgaaac
     3061 ccatttgcag aaacaagggc ttgtgaatta ttggggctat aatgtattag gtaattttgc
     3121 tattgatccg aatttagcag cagataagca aaatcctctt aaagagttta aacagctcgt
     3181 aaaaacatta catcaagcac atatcgaagt gattatggat attgtgttta accacacagc
     3241 tgagagtgat aaaaacgggc cgatgctaag ttttagaggc attgacaata cagcttattg
     3301 gctagataaa cagggcgaat acgaaaattg gactggctgt ggtaatacct taaatttgag
     3361 caatccttat atactctgct gggcaatcga ttgcttaatc tattgggtgg aggagtgcca
     3421 tattgatgga ttccgctttg atcttgcctc aattctaggt agaacaccga gtttttctga
     3481 aaaagcagat tttttcacag cacttaaaag ccattctaaa ttaacacata ttaagttgat
     3541 tgctgagcct tgggatattg gtgtagaagg ttatcaatta gggcaattcc ctgagccttt
     3601 ttatcagtgg aatgattgtt atcgtgatga tatacgccgt ttttggttac aaaaccaagg
     3661 tgattggggg cttttggcac ggcgattggc aggaagtgat gatgtgttta ctaaatattc
     3721 cgtttcaaaa agtatcaatt ttattacggc acacgatggg tttacgttac aagatctcac
     3781 cagctatcag caaaaacata attgggcaaa tggtgaacaa aaccgagacg ggcataatag
     3841 taactacagc aataaccacg gtgttgaagg gcagacagca gatgaattgg taaaaaaaca
     3901 gagagaaatc accgcttgta gtttgcttgc tacgctcttt ttatcggctg gtgttccaat
     3961 gcttttagcc ggtgatgaaa tcggacatag tcaacaaggt aacaataata gttattgcca
     4021 agataatgag atcacttggc tagattggga aaatatgaat cacacacggc tggattatgt
     4081 tcgtagttta atccggttgc gtaaaaaaat cgctttgctt tccaagcaga ataattggtg
     4141 gcaagaggat tcggtacaat ggttgaatcc ttctgctcag ctaatgaata ttgacgattg
     4201 gcataatcaa cataatgggc ttgtcgtttt attacaggaa gaatggctgc ttttattcaa
     4261 tgctaatcga caacctcaac cgtttttact gccacaaggc aactggaaag ttgtgttgga
     4321 gcaggctcaa ggtattttga tgacagacac gcaagcggtt acgctcaata atatgggtgt
     4381 atgcgtactt caaaaaagtg atgtaatgtt cgctaataaa ttggagacac aatatgctta
     4441 accaagaatc aacggctctt gttgcaagac aagatttaac caacgatacc ttagtattaa
     4501 ttcttgcagg aggacgaggt tctcgcctat acgaattaac cgataaacgt gcaaaaccgg
     4561 cagtctattt cggtggtagc cgccgtatta ttgattttgc gttatcaaat tgtattaact
     4621 cgaatttatt aaaaatcggg gtgattaccc aatatgcagc acactctttg ttgcgccatt
     4681 tacaacgtgg ttggtcattt ttaccttatg aacgtaacca atatattgat atgttaccgg
     4741 cacgccaaca gctagatgaa aacacttggt atcgtggcac agcggatgcg gtgtatcaaa
     4801 atatggcgat gatgaaatcg cactatagac caaaatatgt ggtgatttta gcaggggatc
     4861 atatctataa aatggactac actcaaatgc tgaaagatca cgtcgatcaa ggtgcaaaat
     4921 gtactgtagg ttgtatcgaa gtgcctcgct cgcaagcaac agagtttggg gtaatggcgg
     4981 tgaatgaaaa gctgaaagtg aaagagttcg tcgaaaaacc agctgatcca cctgcaatgc
     5041 cgggaaaacc ggattcctcg ctagcatcaa tggggattta tgtatttaat gcagattatc
     5101 tatatgaaat gctggaaaaa gaagcctcta caccgggaac ctcacatgat ttcggtaaag
     5161 atattattcc aaaagcagta gagcaaggtg tgctatatgc ccacccattt gaacgttctt
     5221 gtaaagggcg taatgccagt ggggcaattt actggcgaga tgtcggtacg attgacagct
     5281 actgggctgc ccatatggat ttagtttctg aagatccgga attagattta tatgatgaat
     5341 cttggccgat tcacggtcgt ccgcaacaaa ctgcaccggc acgttttttc tataatcgtg
     5401 cgcgtgccag aaccctagat aactcactga ttgcgggtgg ttgtgtgatt accgatgcgg
     5461 aaatcagtaa ttcggtctta tttaaccgtg ttcacgttga agcggagtcg gtgattgaac
     5521 acacagtgat tttaccgcag gtgactatcg gtaaaaattg cgtaattaag cgcgctgtga
     5581 ttgacagaca ttgtgtaatt cctgatggct tagaaattgg tgtaaatgcc gaagaagatg
     5641 cgaaacgttt tcgtgtcagc aaaaatggca ttgtattagt gacacagtca atgttagata
     5701 aattagcggg aaaagaagct aattcggata ttaaatatac aatttagtaa gagatgatat
     5761 aatgcttcga gtttgtcatt tgaggtcgca agctcgaagt attgttatga ttaaagcgct
     5821 tcaagcggtc ttatttttca actattttac acatagaaaa cggttaaaaa tgaaagtttt
     5881 acatatttgc tctgaaatgt accctttaat taaaacaggc ggtttagctg atgtaatggg
     5941 ggcattgcca tttgcgcaac agcaagctgg taatgatgtc agagtgttaa ttccttatta
     6001 cccgaaagtc gcgcaaactt taggggaaac ggtagaagtt gctactatcg gcacttttgc
     6061 cggaacagtc tctgttcgat ttgcttattt taatggctta ggcgtttacg ttatcgatgc
     6121 gcctcattta tttatgcgtg atggtaaccc ttaccacgac agcaattatc aagactatcc
     6181 ggataactat aaacgctttg ctttattagg ttacattggg gcgcaattag cccaaggttt
     6241 ggatcattgg tggggaaccg ctcaagtttt acacgcacac gattggcaag cagggcttgc
     6301 ttgtgcatat cttaaaagtt ggggaagcaa tgtaaaaacg ctcttcacta ttcataatat
     6361 tgcctatatc gggcgcttta atgccttcca tttagcagag cttggcttac cttgggaatt
     6421 tttccacgcc gaaggcttag aattttacgg tgaaatttct tacctcaaag caggtttgta
     6481 ttttgctgat cgcattacta ccgttagccc gacctatgct cgtgaaatta ctgaggaaat
     6541 tgccggtgca ggcttgcatg gtttactggc aacccgtcaa tatcaaggta gattgcgtgg
     6601 cattttaaac ggggtcgatg ctgaggtgtg gaatccggaa acggatacac atattgtggt
     6661 gaattatcgt gcgaactata tgcacggtaa agccaaaaat aaagcggaac tccaacgaat
     6721 ttttggtttg ccagaagaca aagaagcatt attgatggta atggtgactc gtttaactga
     6781 gcagaagggg gcagatttct tactcgccaa tatagatacc ttaatgcaac ataaggtaca
     6841 gattattgta ttgggaagtg gatcgccaga tttagaaaat gcgctacgtt atttccaaca
     6901 caaatacccg aaccaaattg gggtgaaaat cggttatgac gaggctttat cgcaccaaat
     6961 tattgcaggt ggtgatctga ttctagtacc aagccgtttt gagccgtgcg gattaactca
     7021 actttatggg ctgaaatatg gcactttacc gctggttcgc cgtaccggtg gcttagctga
     7081 tacggtggta gatagccata aagagagcat tgaaaaccgc agtgccaccg gatttgtatt
     7141 tgattaccca gatgcacctg cgtttttaga ggcagctatg cgggcaatca gcttatggca
     7201 gaaacaaaaa ttgtggtcga gcgttcgcca gaatgccctt gctcaagatt ttggttgggc
     7261 aaaagtggca gaacagtacc aaaatttata ccacgaaatt atttaatttg tattcacaac
     7321 atcatttcaa gcggtcgaat ttgcggattt ttttgtaaaa acgaccgctt gcaatattaa
     7381 aaattaggag aatttactca atgagcaggc agtcattaag cccagaaatg atcaaaaatg
     7441 ttaaaaatgc gattttgcac aaaatggtgt tcgctttagg ggtcgaaccc cgtgatgcca
     7501 gtaagcgtaa ctggctcaat gcaaccttac gtgtagtgcg tgatttatct accgaagatt
     7561 ggttacaaac ccgccgtagc caggttgcaa atggtagccg ccgtgtttat tacctttcaa
     7621 tggaattttt aatggggcgt acgttcagta acgcaatgat ttctgaaggt gtgtatgagc
     7681 ttattggcgc tgcgttaaaa gagttagggc aagatctgga agagattatc aatgaagagg
     7741 gcgtccgggc ttaggtaacg gtggcttagg gcgtttagcc gcctgctata tggacagcct
     7801 tgccacaatg aaaattccgg caatcggcta cggtattcaa tatgaatacg gtatgttccg
     7861 ccaagaaatc cgcaacgggg agcaaattga gcaacctgat gaatggttag agaatgaatt
     7921 tgtgtggcca tatctacgtt caagtaagcg tttcccggta cgctttggcg gtaaaacttg
     7981 gcgtgagggc aaaaaagtag tatggcagcc ggaagaagaa attaccgctc aagctcacga
     8041 ccaactgatc ccgggctttg agaccacctc aactaacagc ttacgtttat ggtctgcaca
     8101 cgcatcgggt agagggtttg gcttatctga ttttaaccgt ggcgattatt ttgcagcaat
     8161 ggttaaacaa aacagctcgg aaaacgtctc tcgtgtgctt tatccggacg attctaccta
     8221 taacggtcgt gaattacgct tacgccaaga gtatttcctt tgctcggcat ctgtacaaga
     8281 tattatccgc cgccacgaag cggagttcgg ctcttgtgtg aatttagccg acaaagtggc
     8341 aactcactta aatgacacac acccaaccct tgcggtgcct gagttaatgc gtattttaat
     8401 tgacgaaaaa ggctatggct gggaacaagc gtgggcgatg actcgtaaaa ttttcttcta
     8461 caccaatcac actttgatga gtgaagcgtt agaaacttgg ccggtagaaa tgttagcccg
     8521 tgtgctacca cgccatttag agattatttt cgacattaac gaatatttct tagaggaagt
     8581 gcgtagccaa ttcccggaag atcacgattt gatttctcgt gtttccttaa ttgatgaaca
     8641 aggcgatcgc cgtgtgcgta tggcgtggct tgcggtagtc gcctcagcga aagtaaatgg
     8701 tgtggcgaaa attcactcgg atttaatggt tgaatctatt tttgccgatt ttgcccgtat
     8761 ttacccaaat cgttttacta atgtgactaa tggggtgaca cctcgccgct ggatcaaaat
     8821 tgccaaccca gggcttgcta atattttaga taaatgtatc ggcaataagt ggctgactga
     8881 tttaagtgaa ttggaaaaat tcaacgtgtt tgttgatgat gctgatgtgc aagcacaggt
     8941 ggcagcggtg aaagtagaaa acaaacgtcg cttagccaaa tatgtcgaag aaactcaggg
     9001 aattaaactt aatcctgaag cgatttttga tgtacaagtt aagcgtattc acaagtataa
     9061 acgccagcaa atgaacgtgt tgcatatcat cacgctttat aaccgcatct tgaaaaaccc
     9121 gactgctgat tggacacctc gtgtgtttat cttcgcaggg aaagcggcaa gtgcttatta
     9181 tgcggcgaaa aaagtgattc gtttgattaa tgatgtggca aatgtggtga ataatgaccc
     9241 tcgcattaat gatttaatta aggtggtgtt tattccgaac tacggtgttt cattagcaca
     9301 aatgattatt ccggcagctg atgtttcaga gcaaatttcc ttagcgggta cagaggcctc
     9361 cggaacctct aatatgaaat ttgccctcaa tggtgcttta accattggta cattagacgg
     9421 tgcgaatgtc gaaattttag atcacgttgg taaagagaac atctttattt tcggtaatga
     9481 agtagagcag gtggaagagc ttcgccacaa cggttactca ccgtatcact actacgaaaa
     9541 agacagcgaa ttgaatgagg caatttcaca aattctaaac ggtaaattct caccggaaga
     9601 tccgtatcgc tatcaagatt tgatcttaaa ttcaggtgac tactaccaag cctgtgccga
     9661 ttatcgtagc tatgtggagg ctcaagaaaa agtggctcaa gcctatcgta acaagaaagc
     9721 ttggacacgt tcagccatca tcaatattgc caatatgggc tatttctcct cagaccgctc
     9781 ggtgatggat tatgcgaaaa atatttggca tatcgaaccg atgagcgaag aacaattaaa
     9841 aggcgattct acaatgggca gtattattga aagttcaaat aaactgttga atttaaaata
     9901 atgatttaga gaaaacgagt aatcttaccg tttttgtttt agtcagggat tttgcaaatt
     9961 tctactaaaa attaaccgct tgtcaatttg ccttctccac cgccgtgagc attcccctca
    10021 gtaaattcag ctcattagtt tccagttcgg cacgttggta taaacggcgg agttttagca
    10081 tcactgaatc attgcggata aagcctaatt cggtatataa gcgttcggtg tgagcaaaga
    10141 aatgctctaa ggcttgagcg gttgggtaat ccttatctgc tgcaagcggt tgtttttcgc
    10201 cagaattttg caaataagcc attcggattt cataacttgc gagctgtact gccattgcca
    10261 aattcagaga accataatca ggattggttg ggaaattcaa atggtactgg catttcaata
    10321 attcttcgtt ggttagcccc actcgctcac gtccaaatac aatcgccact ttgccaagat
    10381 tggttcgatt taccactaat tcaccacagt ctcttggttc aatcagggta ttttgcaaat
    10441 ggcgaagtct ggcactggtg ccgatcacca gttggcaatc ggcaatggct tcatcgaaag
    10501 tggcgtagca ttgagcgtta tccaacacat cttttgcccc agcagcaagg gcttcggctt
    10561 gttcatcaag cggttgtttt ggggcgacta atcgcaaatg ggttaagccc atcgttttca
    10621 ttgctcgggc tgcggaaccg atatttgccg ggagtgaagt ttcaacaaga atgatctgaa
    10681 tttggttgag aatctgcatt ggttttgttt cttttttgtt aaatttagcc tgttaatagt
    10741 gtagcaaaaa tttagcctgc ttaaaatttg aaaaaatggg aaataatcgg tttaattggg
    10801 cataattttt tacctatttg ttttatttag tggtacattt agagcgtttt taatcacaac
    10861 aaccatacag gagtctttat gcttattggt gtaccaagag agctgttaga cggtgaaact
    10921 cgagtggcgg caacgccaaa aaccgttgag cagatcaaaa aacttggctt tgacgtactt
    10981 gtggaacaag acgcaggttt taaagccagt tttgaagata atacattttt aaatgcaggt
    11041 gcaaatatcg gcacacaaca agaagtttgg aatgcagaca ttatttttaa agtgaatgcg
    11101 ccgacagaag ctgaaattga gttaatcaaa gaaggggtaa ccttagtcag ctttatttgg
    11161 ccggcacaaa atccacaatt gatggaaaaa ttgcagtcta agaaaattaa tgtgttggca
    11221 atggacgctg tgcctcgtat ttcccgtgcc caagctctcg atgctttaag ttcgatggca
    11281 aatatttcgg gttaccgtgc ggtgatcgaa gcggcaaacg cttttggtag cttctttact
    11341 gggcaaatta ccgcagcagg taaagtacca ccggcaaaag tgttggtaat tggagccggt
    11401 gttgctggct tggctgcaat cggggcggca aatagcctgg gggcgattgt gcgtgccttt
    11461 gactctcgtc ctgaagtaaa agagcaagtt aaatcaatgg gggctgattt cctcgaaatt
    11521 gattttgaag aagaaggtgg ctcaggtgac ggctatgcga aagtgatgtc ggaagagttt
    11581 aaccgtcgtg caatggcgtt atatgccgag caagccaaag aggtggatat tattattacc
    11641 accgctgcca ttccgggcaa acctgctcct cgtttaatca ctaaagagat ggttgattca
    11701 atgaaaccgg gttcagtgat tgttgattta gccgctgcaa ccggtggtaa ctgtgaatac
    11761 accaaagccg gcgagattgt ggtgaccgat aatcaagtga aagtgattgg ctataccgat
    11821 tttcctgcac gtttaccaac gcaatcttcg caactttatg gtactaactt ggtgaattta
    11881 ctcaaactgt tagcaccaaa taaagatggt caaattgaga ttaattttga tgatgtagtg
    11941 ttgcgtggcg taacggtggt gcgtgacggc gaattaactt ggccggctcc gccgattcaa
    12001 gtatctgccc aactgcaaaa acaagcagcc gcaccacaag cggtcaaaaa agaggaaaaa
    12061 cctgcagacc ctcgagtgaa atatggcgtg atggcagcag caggtgtgct gttcctatgg
    12121 cttgcatctg tggctccggc agcgttttta tcgcatttta ccgtattcgt tctcgcttgt
    12181 gtggtagggt attatgtggt ttggaacgtc agccacgcac tacatacccc attaatggcg
    12241 gtaacaaatg cgatttcagg gattatcatc gtaggtgcgg tattacaaat tgcccaaccg
    12301 accggtaatt tctttattga tattttggca tttattgcga ttttggttgc cagcatcaat
    12361 atttttggtg gctttaaagt aacgcaacgt atgcttgcaa tgtttagaaa aggttaagga
    12421 gaaacgaaac tatgtctttt ggatttgtga ctgctgcgta cattattgca gctattctct
    12481 ttattatgag cttagccggt ctttctaaac acgaaaccgc caaagcaggt tgttggtatg
    12541 ggattgtggg tatggcgatt gctttagtgg caaccatttt cggcccgcaa tcacatggca
    12601 cgatttggat cttaatcgca atggcaatcg gtggattcat cggggtgaaa aaagccctaa
    12661 aagtagaaat gaccgaaatg cctgaattag tggcaattct acacagcttt gtcggtttag
    12721 cagcggtatt ggttggtttt aacagctacg gacttcatac caatgcatta atgcctgcaa
    12781 atttagatga aactgcacaa gcggcatttt tagcggaaca agccactctt gccaatatcc
    12841 acaatgtgga agtgttctta ggtattttca ttggtgcggt aaccttctcc ggttcattgg
    12901 ttgcctttgg taaactaagc ggtaaattat ttggtcgtaa agtctcttct gcagcactga
    12961 atattccaca taaacacaaa tggaatttgg cagccattat cgtttctgtt tttttgatga
    13021 tcgtgttttt aaataatccg cacaatatct tccctgtgct gattatgaca attattgcgt
    13081 tagtcttcgg ctggcactta gtttcttcta ttggtggggc ggatatgccg gtggtagttt
    13141 caatgctgaa ttcttattca ggttgggcag ccgcagcggc aggctttatg ctaagcaacg
    13201 atttattaat cgtaaccggt gcattagtgg gatcatcagg tgctatcctt tcttatatta
    13261 tgtgtaaagc aatgaaccgc tcattcatta gcgtgattgc cggtggtttt ggcaatgaag
    13321 ttcagtctgg caaaggtgat gaagaatacg gtgaacaccg tgaaacgaaa gccgaagaag
    13381 tggcagaaat gctgaaaaat gcaagctctg tgatcatcac gccgggttat ggtatggcag
    13441 tggctcaagc acaatatccg gtagcggaaa tcaccgcaaa attgcgtgaa aaaggcgtga
    13501 atgtgcgttt tggtattcac cctgtggcag ggcgtttacc ggggcatatg aacgtattat
    13561 tggcggaagc gaaagtacct tatgatgtag tgctggagat ggacgaaatc aatgacgatt
    13621 ttgaagaaac cgatgtggta ttggtaatcg gtgcaaacga taccgtaaac cctgctgcat
    13681 tagacgaccc atcaagccct attgctggaa tgccggtgct tgaagtatgg aaagcacaaa
    13741 atgttatcgt gttcaaacgt tcaatggcag taggctacgc cggtgtgcaa aacccactgt
    13801 tctttaaaga aaatacccaa atgctatttg gtgatgcaaa agaacgtgtt gatgatattt
    13861 tacgggcgtt aaattaaatt taaataaaaa agcgaacata agttcgcttt tttgtgctta
    13921 ggctaacata aattgttaat tttttactct aataccatca atccgggaag agtcgcaaaa
    13981 ctctgcgatt ttacggtatc atggaaatca tctaagtagg ctagggtagc atcggcttct
    14041 cgaatagcgc cataaagggt gctatatagt ccttgctgag ttatttttct tgccaccaca
    14101 taccccctat ctaaatatgg tttaaccgcc caataaggta gtgcagcaat gcctcgccga
    14161 cttgctacta gctgaataat agcaatagtt aactcactat tacgccgagt aactttgatg
    14221 ccagcagggc ctaatatttt tcgcagtaaa tctagcatat catcaggtac tggataggta
    14281 attaatgtct catctttaaa atcttcagct tcccatacat cttttgtggc aagagggtga
    14341 tctttagagc aaatagctac catttcatat gaaaagagag gtttgtatat aataccagca
    14401 ctatcttcct cttcagcgac aattgccaga tctgcacgat ggcttaatag caaaccaacc
    14461 gtatcagtat ggaaacccga tacgatatct aattcaacta gaggccaatg ttgacggaaa
    14521 gtatccattg ccggcattag ccaatcaaaa caggtatggc attctactgc aacacgtacc
    14581 tctccgactt ctccttgttt cactcgggct aaatctaatt cagcctcaat gactttgggc
    14641 aaaatatcgt aggccaactt aattaaacgt tcgccagctg gtgtaaagcg aataggggag
    14701 ctttttcgct caaatagcgt aagatcatac tgttcttcca gtaattttat ttggtgtgaa
    14761 agtgcggatt gtgttaaatg gacacgcttt gctgcaagag atacgctacc gctctctttt
    14821 aatgcgatta atgtccgcaa atggcggatt tcaagaaaaa tcggtttcat aatttattca
    14881 tctaaaagtg aaaaaagatg aaaataatta taacaaacaa gcggtcaaat tctgcaaaaa
    14941 atttgcaaaa tccaaccgct tgtatagagc agtggcgaat tattgggtta attgaactaa
    15001 aatgcggcga acaggctctg ctgcacccca taataattgg tcacctacgg taaatgcagc
    15061 taagtattct ccacccatcg ctaatttacg caagcgacca accggcacgc tcaatgtacc
    15121 ggtcactttc gccggggtca attcacgcaa tgtggtttct ttgtcgtttg gaattacttt
    15181 cacccattca ttgtgggcag caataatttg ctcgatttcc gctaacggaa tatctttttt
    15241 cagcttaatt gtgaatgctt ggctgtggca gcgtaatgct ccaatacgca cgcataaacc
    15301 atcaactgga attgggttat cgcttaaacc taaaatttta ttagtttctg cataaccttt
    15361 ccactcttct ttagtttgcc cggtttccgg taataattta tcaatccaag gaattaagct
    15421 accgcccaat gccgcaccga agttctcggt tgggaagtct tccgcacgca ttttagccgt
    15481 aactttacgt tcaatatcta aaatagaaga ggcaggattt gctaattcag cttttacgct
    15541 atcttctaat tcacccattt gagaaagcaa ttcacgcata tttttcgcac cggcaccgga
    15601 agcggcttgg taagttgcta cagaaaccca ctcgaccaaa tctttctcaa ataaaccacc
    15661 gatagccatt agcattaggc ttacggtaca gtttccgcct acaaatgttt taatgccgtt
    15721 ttttaagcct tctgaaatca catgttggtt caccgggtct aatacgataa tagcatcgtc
    15781 tttcatacgt aatgcagaag ccgcgtcaat ccaatagccg ttccaaccgg tttcttttaa
    15841 ttttggatag acttcatttg tataatcacc accttggcaa gttacgataa tgtctaattt
    15901 ttttaattct tcaatgtcaa aggcgttttt taattcgccg gcatcttttc cgccaaaaac
    15961 aggggctttt tgacccgctt gtgaagtagt gaaaaaaaca gggttgatat tggcgaagtt
    16021 attttcttca accatacgat ccattaagac cgaaccgacc ataccacgcc aaccgataaa
    16081 acctacattt tgcattgtgt ttgctcctaa aaatgatgtt ataaagattg tgaataaata
    16141 caggatttta gtgcaaaaat caatgttttt tattgatttg caaagttttg acaaaattta
    16201 accgctattg
//
LOCUS       DANLUA010000038        15917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLUA010000038 DANLUA010000000
VERSION     DANLUA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            131..685
                     /gene="pilW"
                     /locus_tag="PXB44_000894"
     CDS             131..685
                     /gene="pilW"
                     /locus_tag="PXB44_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL5281393.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            685..3009
                     /locus_tag="PXB44_000895"
     CDS             685..3009
                     /locus_tag="PXB44_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL5281394.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(3080..3643)
                     /locus_tag="PXB44_000896"
     CDS             complement(3080..3643)
                     /locus_tag="PXB44_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5281395.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            3746..5602
                     /gene="sppA"
                     /locus_tag="PXB44_000897"
     CDS             3746..5602
                     /gene="sppA"
                     /locus_tag="PXB44_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL5281396.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            complement(5643..5819)
                     /locus_tag="PXB44_000898"
     CDS             complement(5643..5819)
                     /locus_tag="PXB44_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5281397.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(5827..6036)
                     /locus_tag="PXB44_000899"
     CDS             complement(5827..6036)
                     /locus_tag="PXB44_000899"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5281398.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            complement(6075..7247)
                     /gene="cytX"
                     /locus_tag="PXB44_000900"
     CDS             complement(6075..7247)
                     /gene="cytX"
                     /locus_tag="PXB44_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL5281399.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            complement(7240..8769)
                     /gene="thiE"
                     /locus_tag="PXB44_000901"
     CDS             complement(7240..8769)
                     /gene="thiE"
                     /locus_tag="PXB44_000901"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL5281400.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     regulatory      complement(8837..8933)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9069..10382)
                     /locus_tag="PXB44_000902"
     CDS             complement(9069..10382)
                     /locus_tag="PXB44_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5281401.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     gene            complement(10375..11193)
                     /gene="thiM"
                     /locus_tag="PXB44_000903"
     CDS             complement(10375..11193)
                     /gene="thiM"
                     /locus_tag="PXB44_000903"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL5281402.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     regulatory      complement(11250..11346)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11403..13184)
                     /gene="nrdD"
                     /locus_tag="PXB44_000904"
     CDS             complement(11403..13184)
                     /gene="nrdD"
                     /locus_tag="PXB44_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL5281403.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     gene            complement(13181..13654)
                     /gene="nrdG"
                     /locus_tag="PXB44_000905"
     CDS             complement(13181..13654)
                     /gene="nrdG"
                     /locus_tag="PXB44_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL5281404.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            complement(13867..14085)
                     /gene="ilvM"
                     /locus_tag="PXB44_000906"
     CDS             complement(13867..14085)
                     /gene="ilvM"
                     /locus_tag="PXB44_000906"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL5281405.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            complement(14095..15747)
                     /locus_tag="PXB44_000907"
     CDS             complement(14095..15747)
                     /locus_tag="PXB44_000907"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL5281406.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
BASE COUNT         4564 a         3437 c         3577 g         4339 t
ORIGIN      
        1 aacaccccat tattattttt gcgatctgcg tcgaaaaatg aaaacgaaaa atttttcttc
       61 ttcgcaaaaa atcgctagtc ttagcgagga tggaacagta aaattaagcc ctttgttgta
      121 ggagaacgtt atgtttgcaa aattttttcc aaaaatgacc gcttgtattc tgattttttt
      181 attagcggct tgttcttctc ttcccccttc ttcctctgaa gtcgaattta accgctcgga
      241 agcggtaaaa gcccgtatta atttggcttt agcttatctg gagcaaaatg atttcccaaa
      301 agctaagcag aatattgata aagccttggc tcacgatgaa aaagattacc tcccttattc
      361 ggtgcttgcc tattattacc aacaaacagg tgatgctgaa aatgccgaaa aagcctacca
      421 aacggcactg aaattgagtc aaaaccgccc tgatgtgctg aataattacg gcactttttt
      481 atgtaaacag ggtcaatttc aacaggctta tcagcaattt gagcaggctg tcaaaagcaa
      541 agcaccctac tatcaccaag cggatagctt agaaaatatg gtgttatgtg cgaaaaaaga
      601 gccgaatctg caaaaaattg ccgaaacatt gaccgagctt gagaaattgg ataaacctcg
      661 agcaaattta ttgaaggaaa actaatgact gacttaaatc taaaaatcag ccaaattatt
      721 gccggcgaac tcaacgttgg ctcacaccaa attcttgcag ccatcacgct attaggtgag
      781 ggcaatacca ttccatttat tgctcgttac cgtaaagaag tgaccggcgg gctagacgac
      841 acccaacttc gccattttga aacccgctta atttatttgc gtgaaatgga cgaacgccgc
      901 caaaccattt tgaagtcgat tgaagaacaa ggcaaattaa ccgatgaatt aaaagccaaa
      961 attctaagca gtgaaagcaa gaccgagctg gaagatctct atttgccgta taaaccaaaa
     1021 cgtcgcacca gagggcaaat tgcgattgaa gcagggttag agccattggc ggacagttta
     1081 tggaatgatc cgaaacaaga tcctgaaacc ttagcggcaa actttgttga tgcggaaaaa
     1141 ggcgttgcgg ataccaaggc cgcattagac ggggcaagat atattttaat ggagcgtttt
     1201 agcgaagatg ctgagctact tgcaaaactt cgccaatatt tgaccgctta cgccacgttg
     1261 gaatcgaaag tgattgacgg caaagaggaa gagggcgaga aattccgtga ttattttgct
     1321 catagcgagc cgtttaacag cgtgccttcc caccgtgctt tggcgatgtt ccgtgggcga
     1381 aatgaagggg tgttatcact ttcattaaat gccgatccga ctgcagaaga aggcacaaaa
     1441 tccagccatt gtgaagaaat tattcgtgag catttgggcg tgatttttaa taatcaacca
     1501 gcagataaat ggcgtgagca agtgattgct tggacgtgga aaatcaaagc agctctgcat
     1561 ttagaaaccg aattaatggc aaccctgtgt gaaaaagctg aagaagaggc gattgatgtg
     1621 tttgcccgca atctctcggc attattaatg gcagcccctg ccggtgcgag aaatacaatg
     1681 gggctagacc cgggcttgcg taccggcgtg aaagtggcgg tggtggataa caccggtaaa
     1741 ttgctggata ccgccacaat ttaccctcac actgggcgag aggcggaggc acagcttgcg
     1801 attttcagct taatcaaaca acacaatgtg gaactgattg cgattggtaa cggtacggcc
     1861 tctcgtgaaa ccgaacgttt tgccaaagcg gtgattaaag aaatcaaaga gaataaaccg
     1921 caaacggtgg tggtgagtga ggctggggca tcggtctatt ctgcctctga atttgcggct
     1981 aacgagtttc caaatttaga cgtttcatta cggggagcgg tttcgattgc ccgccgtttg
     2041 caagatccac ttgccgagtt ggtgaaaatc gaaccaaaag cgattggtgt tgggcagtat
     2101 caacacgatg taaaccaatc ccaacttgcc cgtaaactcg atgcggtagt agaagattgt
     2161 gtaaacgcag tgggcgtgga tctgaataca gcctccgctc cgttgctggc tcgggtggcg
     2221 ggaatgacca aaacattggc acaaaatatc gtggcattcc gtgacgaaaa cggccgtttt
     2281 accagccgct cagagctgaa aaaagtgcct cgtttagggc cgaaagcctt tgaacagtgt
     2341 gccggtttta tgcgaattat cggtgggaaa aatgcccttg atgcctcaag cgttcacccg
     2401 gaagcttacc cggtggtaga aaaaatctta caggcaacta cctcaacact tgcagaatta
     2461 atgggtaaca acagcaaaat tcactcgctc aacgccaaag attttgtgga tgagcagttt
     2521 ggtttaccaa cggtgaacga tattttcaaa gagttagaaa aaccggggcg tgaccctcgt
     2581 ggagagttta aaaccgccgt gtttatggac ggcgtggaag aaattaccga cctcaaagcc
     2641 ggtatgattt tggagggcac agtgaccaat gtggcaaact ttggggcttt tgtcgatatt
     2701 ggcgtacacc aagatggctt ggtgcatatt tcaatgctct caaactcttt tgttgaagat
     2761 ccgcacacgg tagtgaaagc tggtgatgtg gtgaaagtca aagtgcttga ggtggacgct
     2821 gcccgcaaac gtattgccct tacgatgcgt ttagacgaca aaccaaccga taaaaaagag
     2881 ccaacaagcg gtcgaaattc agcagaaatt tgcaaaaata cgcagaaaaa tgaccgcttg
     2941 caaagcaaca atcgcaacgc ttttggtaac aatgcctttg ccgatgcgtt aaaaggctgg
     3001 aagaagtaag gcgaaaacag ataaaagcaa gggcgaaata tttttcgccc ttattactaa
     3061 cttttatctg ttaacctgct tacttaagat aacgtacaaa cccctctaaa ctttcacttt
     3121 caggggctaa tgcaatttgc tcgccctctg ccggtgagcc aagcataatt aaggcaatga
     3181 ttttatcaga ttcttggcaa ccaaaagcgg agcgaagagc agagccgtta atccatttat
     3241 tggtgatcca aaccgtatca aagccctgtg catttgccgc taactgaatc gcataagttg
     3301 cacagcctgc ggtaatcatt tgttcccaac ttggcacttt ggcgatgctg tcgtctaatg
     3361 ttgctaccac gccgataatc attggagctt gattagctaa tttttccgct ttttttaagc
     3421 gttcctcgcc catatcaaat tctgctaccg cagactttaa gcattcacca aactgtggca
     3481 taccgctttt ttcaatcacc acaaagcgat aaggctttaa ccgcccgtga tccggcactc
     3541 gcaagccggc ttttagaatt tgttccagtt gttctgcatt cggggcttta tcgccaaatt
     3601 ttttatttga acggcgatgg tgtaataaat ccaagatttg cataagcgtt cctttgttaa
     3661 ttttttgcgt tcaagaatag catattttct tcatttagcg attgctttat actgtgccga
     3721 ctgagtttat ttttaggaat ttccgatgtt aacgatttta aaagcatttt accgaatctt
     3781 ccgttgtatt agagagtgtg tcattaatct cttttttatt ctgtttattt tgctattagt
     3841 gccggtggtc gcatttatca gctcttctgg cagcacgcaa aagccggtgt tcaatcaagg
     3901 ggcgttacgt ttaaatttag atggttattt agcagataat cgggaagaat tcactgattt
     3961 ctaccgcttg gtgcaatcgg aattaggtag ttccgagccg tttaaaatct cgacttttga
     4021 tgtggtgcaa gcgattagcc acgctaaaaa tgacccgaaa attaccggct tggtgttaga
     4081 tctgcaaaag ctacaaagtg ctgatttcag ctcattggat tttatcggca atgaaatcaa
     4141 caacttcaaa caatcgggca agccggtgat tgcagtgggc gaagactact cgcaaaaaca
     4201 atattatctt gcttcctttg ctgatgaaat ttatctcaat aaagtcggtg cggtggattt
     4261 aaaaggctta aattattcca acacttattt caaaaccttg ctcgataaaa ttgaggctga
     4321 accgcatatt ttccgtgtcg gtacttataa atcggcggtt gagccgttta tgcgtgatga
     4381 tatgtctgag gaggcgaaac aaaatgcccg aggctggtta aacggcactt ggcaacaaac
     4441 tgtggcaaca ctagctcaaa accgcaacat tgaaccgcaa gcactggatt tatcgcctgc
     4501 caattacatt gaaaaatata aacaagctaa aggagatgat gctcaactgg cattaaatca
     4561 aaaatgggtt agtcagttag tgtccagcca agaaagtcgg gataaactga ttgcacagtt
     4621 tggcaaaaac agcgaaggca gttataacca gattgaattt ttagattata tgactgaact
     4681 aaacgaccgc tttgcagaag tgaatgctcc gaaaattgcg gtagtgaatg tggaaggggc
     4741 gattgctggc ggtgaaagtg acgaaatgaa tgtgggcagc gagacgctgg taaaattact
     4801 gcaacaagct cgagatgaca aaaatgtgga aggcttaatt ctacgcatta acagccccgg
     4861 cggcagtgct gtggcgtctg aactgattcg ccaagaagtg gaagcctttc aacaagccgg
     4921 caaaccggtg gttgcttcaa tgggcggaat ggcagcatcg ggcggttatt ggattgcggc
     4981 taccagcgat aaaattattg ctagcccgaa tacgcttacc ggctctatcg gtattttcgg
     5041 tttagcagta acttttgaaa aaaccgctaa aaatctcggt gtgagcgaag acggcatagc
     5101 gacttctgcc ctagcacaac aagccggttt aaaaagccta ccgcaagagc aggcagaagt
     5161 gctgcaaatt gggattgaaa acggctacga ccgtttttta gagctggttt ctcgtggcag
     5221 gaaaatgcca aaagaggtgg tagataaagt ggctcaaggg caagtgtggc tgggaactga
     5281 tgcgttaaaa cacggattgg ttgatgagtt gggcaatttt aatgtggcat ataatgcgtt
     5341 atctgaattg atcaaccaaa aacgtgaagc ggaaaaccaa cctaaagtcg aacagttcgg
     5401 cttacaatgg tttgcggagc aagatgacag cttatttggc acgcttgccc gtgatttcaa
     5461 agctcaatta caatttagcg tggcaaattg gcttgattta ccgctgttga agcaaccgca
     5521 acaacagcta gagactcttt cacgttttaa cgatccgaaa cagatgtatc tttactgttt
     5581 aaattgtggg aagatagatt aattattgaa tttaagggca ggttttcacc tgccctctta
     5641 aattaccatt gataacgata atttactaat acattaaatg gttgtttcag tttcttacct
     5701 gaagcaactg atgcctctaa acctaaacgg tgattaccgt ttcctgcact caagccaagt
     5761 gaaccttgca ctctgccctt gttttcggca actccaaact gatattggtt tacttgcact
     5821 ttcgtactat tggccttttt cagcaatgcc gtttacttcc gcataaggtt gcagattcca
     5881 accattattc aaggcaaagc cttttgctac tcttacgccg gcacgggcaa ctaaaagatt
     5941 taaccgttcc tcatcggctt taccgttaat ggtgtggtag gcaagttgag cttgtggtgt
     6001 tactacccag tcattgttta aaacaagcga ctcaatttta gcaaattttc actccaattg
     6061 aaccgcttat ctttctattt tgtgaacctc ctcacaaaaa tggtgctgag aaaaatcact
     6121 aacatcactg gaatggttaa gccgagtagt aaatgcactt ggtggtaaga gagaaggtgg
     6181 tatagcacga agccgacaag ccataatccg gcagcaaccc aatcaacatt aggctgtgct
     6241 tggtggcgtt ttaacacaaa gaaatccgca atttgcacgg caatcatcgg ggcgaatacc
     6301 gagcctatca gcagcaggaa attttcatat tgggtaaccg gtaaactgat tgccagtagc
     6361 gtgccgatga ttgtgactag aatggcggtt ggggtttctt tgaattttgc cgtgatattg
     6421 ccggcactga caccggcgga ataggcatca agaaacgtgg tagtcacagt cgaaagaatc
     6481 atcaccataa tgccgataag gctcattcct gtcattagta acataggggc aatttcggtt
     6541 tgcccggtaa atagcaccat acccagacct aacgcataca tccacgcact ggtgatcaaa
     6601 tagcctatgg tcgccgaaag ggtcgcggca aaaggctttt tcgctttagc ggtatagtct
     6661 gataccaaag gtagccacga aagaggcata atagttgcta attccactgc ggtgctaaac
     6721 ggcatttcac ccgaaacagg aaaattttcc tgcccattca ataccttaat gcttagccat
     6781 aaggtgagca aaaacatcgc tatcaatgtc actaacttga attttccgac ctgattagag
     6841 ccggtaaata accatagcac aatcagcaac ccaagcacga ttgcccaaag cgagaaagcc
     6901 gaatattccc ataaggcaag gctaagggca ttactgatct ctgcccctgt atagatcata
     6961 atggctgtcc aaccaacaag ctgtagcaca ttggcaaggg agaaaaacac cgagcctctg
     7021 ccaaaggaaa tttgcaccgt ttgcatcgca tttttgccgg ttttcgcccc aattaagccg
     7081 gcagcaaaaa acaaaatgcc gcctaataaa tgccctagca ctatcgccag caagccttgt
     7141 tgccagccaa gtggggcaat ccaagtgcca accacgattg cactggcact aatgcccgct
     7201 cccgcccaaa tcaaaccact gtttagcgta ctaagagttc tattcattac ggcctccatt
     7261 tgcaaatttt gctaagtttt tgaccgcttg ttgccaatct tttgcttggg taacggcact
     7321 taccaccgcg gcactgccga caccggtatg cattacattt tcaatgcttt gctcattaat
     7381 tccaccaatc gccacagttg gaatgttaag cggtgcaacg agagcaacat atttggctaa
     7441 atttgttaag ccttgtggtt gagacggcat tactttggtt tgagtaggga aaacgtggcc
     7501 aaaagcgata taagacggtt tcaccgccaa tgcccgcatt atttcaaagt agcagtgggt
     7561 tgaaacacca agccgtaacc cagattgctg aattgcattt aaatcggcgg tggctaaatc
     7621 ctcctgccct aaatgcacgc cgtaagcctg atacttcatg gctaactgcc agtaatcatt
     7681 tacaaacaaa cgcacattgt gctgtttcgc cagtgcgacc gaatgggcaa tttcttgctc
     7741 aatctctgca agcggtcgat ttttgattct aatttgcaag gtttttaccc ctacaagaat
     7801 taagcgttca agccatttaa cgctatctac aacagggtaa agcccaagtt gatattccgt
     7861 tggagcaaaa ggctctttcg gcaatttaag aggggcagaa atcagctcgg tttgtgggaa
     7921 gaaagcgata tcatcagggt agccggtgtg gcaaagtgaa cttaaagcag tttgccctct
     7981 gccttgtttt tccgataagc ctttttgcaa ataagcggtg gcaacagtga cggcatcagc
     8041 taaatcaaag ccggttgcca acatcgctcc aaccgctgaa gcataggtgc aacctgtgcc
     8101 atgggtattc acactttgct gtcgggcgtg actaaaggca taaatttcat ttgcagtcag
     8161 cagataatct cttaccgttt cccccagcca ttcaaaatgt ccacctttgg ctaacacggc
     8221 tttgattccc tgtgcaaaca gtttgtggct agcggttttc acatcaccaa aggtctggat
     8281 ttctgtgtcg cttaataaag cgagttcagt gccgtttgga gtgattaaat ccaccagcgg
     8341 atacaatttt tgttgcacta tctgcaataa aatactgtcg gataacgcct gcccactgct
     8401 tgccactgcc acagggtcgt aaaccacata acaataatgc gagctttctg cacggatttt
     8461 ttggatatat tcacacaaga tctctacttg tgcttgcgag cagagtaagc cgattttgat
     8521 tgcgttcggg tagccttgaa ccagcaaggc ttcaaactgt tggcggaagg tgtgatcatc
     8581 aacaggcgtt acacacagca caccgtgcgg atgttgagcc gttacagaag taactactga
     8641 acaacccact aggttaaaat cgtgaaaggt atgtaaatcg gtttgcagac cggcaccggc
     8701 acaggaatct gagccggcaa ccgtccaaat gacgttaggt tggtatggag agtttgaata
     8761 attagacatc gcaaaatcct cataatatgc ttagggtttt gcttgccaga gatgtggtaa
     8821 tgatcccaaa atctgaattt gggtaaaact tgttccctac gcaggtacta acctacaggt
     8881 tccacggatc tcgctgttgc gatctcagcc ttacggcact ccgacaagcg tttttagtat
     8941 agcttacaag cggttatttt ttataaaaaa atgcaaaata tccgttgtga agtctattag
     9001 agggaaattt gcattccctc atcttggggc aaggtggtaa cttgccccgt cgttaagtga
     9061 gtggtttttt atatctcctc taattccaaa tctttggttt cgtggacttg ccacaatgcc
     9121 cagaagctca gtaatgaggc aaccgataaa tagccgccta caccgaataa gccgaaattt
     9181 tcattgattt tgaccgcaat aatagtgaac acgctagctc ctaaaatacc ggcagcggtg
     9241 taggcaagcg aagcccctgt gtaacgtact tctgttggga agagttcggg tagaattact
     9301 gccatcgggc cgaagcttaa gccgattaag atcattccaa tggtgaggaa aataaagatc
     9361 gaagtcggtg tgccgttttc gaggaaaaac ggcattgtta agccgtaaac tacggttgct
     9421 cctgtggcaa gtaacagcag taaacggcgt ccgattttgt ctgaataaat gccggagaag
     9481 aaaatcgcaa tgccgaaaat cacggaggta atcagcaaat attgggtgta gagatttacc
     9541 ggtaaaccta agcctgtagc gtgtcctgct tgagaaactg ccaccgggct tttgacgaaa
     9601 atcggggtga aagcaactaa aatgtagaac aacacatagc cggcggtggc aatcagcacg
     9661 ccgatcataa aaggcttgag gtgttggcta aacaaagttt taagcggggt atggcggttt
     9721 ttaccttgtt gttccgcttt aaggaaaatc gggctttcgc tgatcttaat tcggatataa
     9781 agcccaatgg cgatcatcac aatagaagcg acaaacggaa ttcgccacgc ccaatccaag
     9841 aaggtttgtt caccgtaaat taaattcacc aagtagaacg ctccgtttgc caacagcaag
     9901 ccgattgggg cgcccatttg cggaaaaata ccaaaaaatg cccgcttgtg tttcggggcg
     9961 tgttcagtgg caacgagagc agctccgccc cattcgccgc ctaagcctaa accttgtccg
    10021 attctacaga ggcagagtaa cacggttgcc caaatgccaa tggcggcata attaggcaaa
    10081 agcccgattg ctacggtaga taagcccatt aataagagcg aggcaatcag agtttgtttg
    10141 cgtccgtatt tatcgccgaa atgcccgaat aaaatcgacc ccatcgggcg ggtgaaaaag
    10201 gcaagagcta gtacggaaag cgacattaat tggttggaaa ccggatcatc gctattaaaa
    10261 aactgcacat taaacaccag cactgctgcc atcgtgtaga tgtagttatc aaaatattca
    10321 atcgccgtgc cgaccattga ggcaaccgcc actttagttg ggtttgagtg agttttagac
    10381 atattcaacc ctcacatatt gctcaacggt ttcaggcgtt aatgccccaa gggtatccag
    10441 cagattgacg gcaaattgcc cgtattgagt tggtttgagt gcttgagcag ccacttcgcc
    10501 tgcaacggca taagcggcgc aagcctcaat aagtgcgttg aaatagtgct ctttgtcggc
    10561 aacggctaag aatgccccgc ataccgcact taataaacag cctgaagcgg taattttggg
    10621 gaacatcggg ctgccgttgt gcaataaagc tgtgtgtgag ccagagctta ccacatcggt
    10681 ttcaccactg attgcgacta aggtttggta acgtttggca atctgttggg cgatttcaac
    10741 cacattatgc gagccttcgc cggcatcaac gcctttggct tgccattcta caccggcgag
    10801 ggtagctagt tcccccgcat ttccacgaat ggcggtgaag tgaatttggt cgactaaggt
    10861 ttcaactgtt tgacggcgga aaggtgttgc tccaacgccg acagggtcaa gaatgagcgg
    10921 aatacctaaa cggttggcgg ttttgccagc aagctccatt gcttctactt ctttgccgat
    10981 aagcgtgccg atattaatca ccacggcgtt actgagtgcc ggcacggatt ccatctcctc
    11041 aatagcagct gccataatgg gagaagcacc aagagccaat aagccgttgg cagaaaaatt
    11101 agcggcaaca atgttggtaa tgttgtgaac aagcggtcga ttttgtcgaa ttttttgcaa
    11161 ataatgcgag tgatagggga gtgatgtggt catcgtttat tccttaatat gattgaaaaa
    11221 caacgcacca acaccaacgg ggaatagatg ggtttacgcc tttagtttcc tacgtcagtg
    11281 ctaactgcat caggttcaac gggtatttct cagcttgccc ttaggcaagc accccgacta
    11341 aatgaggctt taatataatc actaaaaaag cggttgtaaa gggctacaac tgcagttttc
    11401 aattaagctg cttctcggtg atagaaacgc aagctgtgct ctttttggcg ggcggaacta
    11461 aaggcggaga ctcgttttaa atagccgatt acccgagtgc cgtaatcaat attatcggag
    11521 ccacatttgc tacaactgtg caacgtgcgt ttgtcgatgt ggctacattc gttgcaaatc
    11581 gtgattttaa cgttaataca aaaataattg cagccggttt tagcggcggt atccagcaag
    11641 gagcgatatc cggtttgggt taaggcttcg tctaaattca agtgtagagc ggagcctccg
    11701 tccagccatt caatcagctc tctgccgtgc agtaagaatt tatccaaagc attaatttct
    11761 tcatcttcta ccacatagaa ataggaattg tagcaatctc gtggtacgaa atagccgtcc
    11821 tctttgtccc atttggcgtt tttgacccct aaattttcag caggcacaaa ttcggtatta
    11881 aattttaccc catacttttc accggctgct tggttagctt ggaaaataat ttttaaccga
    11941 gattgcacaa aatcaatata gtctggattg taacctacgg ttaaaccttg tgattcagca
    12001 gcttctgcca ttccgttaat gccaatagtc aggaattgtt tatgcagtga aataaaaccg
    12061 gcatcgtaaa ccggcaacat accggcagct aaatactctt ccatcagttt gcggtaagcg
    12121 tattgatatt tatggatctt gctgacttct tcgcttaaat cacggttatc ttgcaccaaa
    12181 cggttcatat taatagtaat tacattgatt gaacctgtcg caacacctcc agcccctaat
    12241 gaataggaga agctatgatc gctgatttca tttctcaagc ggcagcaaga agcaagggaa
    12301 tccggattat ctgattgata aataaagaat gaattgcctt gagatagctc ttttgccatt
    12361 tgattggcaa actcgtcatc tttacatttg cccttttcgg taagcattgc tgcggtcaca
    12421 acagggaaag tgagaatcgc tttgcttcgc tctttattga accattgcat aaagaaaagt
    12481 tgtaattttg aagtggtttc ccaatttggt ttctcaaaat caggaaaaac aaaacggcta
    12541 aacatcgctt caaaatagaa tttgtcatac actgaaatat tccaaaagac gctttgataa
    12601 ccacgagcgg ctgccggttg gttgatgcta tacacaacct gctgcaggtg gttttcaata
    12661 tctgaacgat gagtctgtaa gtaattatcg ccgtaatctt tgcgggcaaa atagtcaaaa
    12721 taggtcagaa attcaaccgt ggcgactgca ccggcaaact gagaactgac tgcaaagaca
    12781 aaattgataa aagaaccgca gaatgaggct aaatgtttcg gggctttgga ttcgccgccg
    12841 agtttagtca aaccatcacg caagaaaggg tacatagtga ctgaaacgca gtagggtttt
    12901 aggctggttt catcgtgtac gtaaatttcg tgtgcttcga tttgacgtaa atattcacaa
    12961 gcggtttctt ttccaaaaat ttttgcaatt ttttcactca cttttgctcg gttgatttgc
    13021 acaaaaaaat ctttcattag ctcctgttcc attgtcgcta tgtttttttg cgtgacgtta
    13081 gcgttggcgt ccatttttga accgtctgcg gcatttttgg cttgaatata ttggttaatg
    13141 aaatgtagtt ttccgtctaa ttgtgtcggt tgtaagcgaa tcatattgaa gctcctcgaa
    13201 taaaaagatg gttaagattt tggttattgc gtaaatcagt aaagcgttgg ttggtggtta
    13261 aactttccaa tcctcctcgt tcggcaatcc aacgcccggt ttttaagtag gttagctcgt
    13321 gtaataaagt ctcggcgatt tcgtgctgct ccaaaccact ataaagacag gtgtttagcc
    13381 cctgtttttt tgccagttgc aacagcggca gtaatttttc tgcttcccat tctccgccca
    13441 taaacaatac gcagctaatt aagccttggt agcgttttag gcgttcagag agatattcac
    13501 tgctaagctc tttcccaaga caggctttcc agctatccgc actatggcaa cctttacagc
    13561 caagcgggca gcctgtaatc agaaaagcaa gtgaggtctc attcggcact tcttgccaaa
    13621 caatttgttc cgagctgaat ctgagatttt ccattactaa ttcattaaca caatatattg
    13681 tgttttttaa cattatttaa cactatatat agtatgttag atctgttgaa tgggaaaata
    13741 aagagcaata agtgaaaaaa aagagttgaa atttgaagaa atgaataaaa tcaaaaggat
    13801 agcgaatgat aaattttaag cataaaaaag ccgtgtattt ttttacacgg cgaatgaaaa
    13861 gtctgattaa ttgatttcaa tgatatcaaa cagtttttct aattgatggc ttaaaagaga
    13921 gagtggacgt ttacttgcta atgtaaaatt taggttaaag gcgctgtctg ttgattctac
    13981 atttaagcat ttcacctcaa aaccacggtg acgtataacc cgtaaaatac gctctaaggt
    14041 ttcagggcgt ttgttagctt taagcgttaa ttgatattct tccatcttat tctcctatac
    14101 ctcatcgtct aacatatcgg cattacacgc ccccggcggt accagtggcc aaacgttatc
    14161 ttcctgtgga atacaaacgt gaagtaagta agcccctttg gcattgaaca ttctgtctat
    14221 tgcatctgac acttcgctcg ctttctcaat tcgctcaccg tcaatatcaa aggctttcgc
    14281 caacaacaca aaatcagggt tatcatctaa aatggtttgg ctgtgtcgcc cgtggaagaa
    14341 cagcgattgc cattggcgaa ccatacctaa acgttggtta tccagtaata ggattttgac
    14401 cgctaaatca gctcttttaa gcgtgccgag ttcttgaata ttcatcataa tcgagccatc
    14461 accggtaatt aaaataacct gatcttccgc tcgagctttg attgccccaa tcgccgcagg
    14521 caagccaaaa cccatagagc caaaaccggc agaggtgata tagtttttcg gatcgtgata
    14581 agttaaatgt tgtgccgacc acatttgatg ctgccctaca tcggtagtga cgatgctgtt
    14641 tttggctttc cgctgtgaaa gcgtgtgtaa taacgccacg gcatcaatat tgccatcacc
    14701 actattttct tgatattgaa agtcaaattg ctgctttaaa ttacgcacat cttcacgcca
    14761 tggttcaata tctaacggtt gagccaagta attcacggct tcaatcaaat tcccttttaa
    14821 ggcaacttgt gcttgacgga gcttgttgat ttccgcattg tcgatatcaa tgtgaatcac
    14881 tttagcgtga ggggcaaaag tgtctaattt gccggtaaca cggtcatcaa agcgggcacc
    14941 acaggcaatg agtaaatcac actcttgtac cgcgtaattt gctgcttttg tgccgtgcat
    15001 accgatcatt cccatataga gcggatcggt tggatcgata gtgcctaacc cttttaaggt
    15061 agaaacagac ggaatatttg caatttttac aaaatttctg accgcttgta caccatcagc
    15121 catactaacg ccaccgccga cataaagtac cgggcgtttg gcggtggcta ataattgttt
    15181 ggcaaccact aatttgttgg gatcttgtgc atcggcaggt tctttaggat aaacgatagg
    15241 cagagccgaa gtttgagcta gttgcacatc acgtggaata tcgaccagca ccggaccagg
    15301 tctgccactt tgtgcaattt ggaatgcgag ggctaaaatt tcagggagct cctcaatatt
    15361 ctgaacaata aaactgtgtt tagtgcaagc gagtgacaaa cctagtacat cagcttcttg
    15421 gaaggcatca gtgccgatta aatggctggc aacttgtccg gtaattgcta ccacaggaat
    15481 agaatcaagt gcggcatcgc ctaaaccggt gattaaatta gttgccccag ggccggaggt
    15541 cgcaatacaa acccctactt tacctgttgc acgggcataa ccgatagctg ccattgcagc
    15601 accttgttca ttgcggcaaa gtaaatgatc taagcctgaa tcataaattg catcataggt
    15661 aggcataata gccccgcccg gatagccaaa aagtgtcgtt accccgtgag cttttaaact
    15721 ctcggtgata aatcttgctc cattcatgga tgttgtgtcc ttaatatttt tattatttta
    15781 aaaccaggca atatagataa cataaattcc ctgtttttta aactttattt tcagtaaaaa
    15841 taaataggat tccatcattt atttggacaa ataaaagtcg tttttattta ggtagagaaa
    15901 ggaattacta cttaaga
//
LOCUS       DANLUA010000039        15710 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLUA010000039 DANLUA010000000
VERSION     DANLUA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(52..1092)
                     /locus_tag="PXB44_000908"
     CDS             complement(52..1092)
                     /locus_tag="PXB44_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5281407.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1181..1771)
                     /locus_tag="PXB44_000909"
     CDS             complement(1181..1771)
                     /locus_tag="PXB44_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL5281408.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXB44_000910"
     CDS             complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXB44_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL5281409.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXB44_000911"
     CDS             complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXB44_000911"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL5281410.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4331..4942
                     /locus_tag="PXB44_000912"
     CDS             4331..4942
                     /locus_tag="PXB44_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL5281411.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4946..6430
                     /locus_tag="PXB44_000913"
     CDS             4946..6430
                     /locus_tag="PXB44_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL5281412.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6431..6958
                     /gene="hemG"
                     /locus_tag="PXB44_000914"
     CDS             6431..6958
                     /gene="hemG"
                     /locus_tag="PXB44_000914"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL5281413.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7090..7485
                     /gene="mscL"
                     /locus_tag="PXB44_000915"
     CDS             7090..7485
                     /gene="mscL"
                     /locus_tag="PXB44_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL5281414.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXB44_000916"
     CDS             complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXB44_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL5281415.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
     gene            8125..9699
                     /gene="murJ"
                     /locus_tag="PXB44_000917"
     CDS             8125..9699
                     /gene="murJ"
                     /locus_tag="PXB44_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL5281416.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
     gene            9701..10168
                     /locus_tag="PXB44_000918"
     CDS             9701..10168
                     /locus_tag="PXB44_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL5281417.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(10233..12242)
                     /locus_tag="PXB44_000919"
     CDS             complement(10233..12242)
                     /locus_tag="PXB44_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL5281418.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            12434..12969
                     /locus_tag="PXB44_000920"
                     /pseudo
     CDS             12434..12969
                     /locus_tag="PXB44_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12997..13608
                     /locus_tag="PXB44_000921"
     CDS             12997..13608
                     /locus_tag="PXB44_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5281419.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(13619..14509)
                     /gene="rarD"
                     /locus_tag="PXB44_000922"
     CDS             complement(13619..14509)
                     /gene="rarD"
                     /locus_tag="PXB44_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5281420.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(14591..15574)
                     /gene="epmA"
                     /locus_tag="PXB44_000923"
     CDS             complement(14591..15574)
                     /gene="epmA"
                     /locus_tag="PXB44_000923"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL5281421.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
BASE COUNT         4408 a         3144 c         3478 g         4680 t
ORIGIN      
        1 taagtttcca cgcttaatag ttacatcgtt taagttgagc atagaaaatc cttatcgttt
       61 cgccaataca atccagaaaa agaactgcac accgactaat gccgtaataa taccgaccgg
      121 aaataatgcg cccggcacaa ggctttttga tagcacggat gacatcgata aaaatgccgc
      181 acccactaac attgaaccgg gcaggaaaaa gcgttggtct tcgcctagca acattcgggc
      241 aatatgcggg gcaactaagc caataaagcc gataatgcct acaaagctaa tgcaggtggc
      301 cgtcattaca gacacaatca gcaatgtttt cattcgtaac caagttaagt taatgcctaa
      361 gctcaatgct cgagcttcgc ccaaacgcaa ggcagttaat ttccacgcat ctttgaataa
      421 taataaaata cagactgtag ttacgccaaa ggcaattgag atagtctgcc aagtagcttt
      481 agttaaatta ccgaatagcc aaaacagaat ttgttgggat acctcaggtg cagaaataaa
      541 ttgcaccaat gacagtaaag actgaaataa aaacaataag gcaattccta ccagcactaa
      601 tattgccgag ctaaaacgtc ctaaagagga aaaggcaaat aaaatggcgg aagaaaatag
      661 tgtcatcaca aaagccccga caggtacaga aatttcggtg ggtaaaccaa atccaccaaa
      721 ggcaatcaca atcgaagcac caaagccggc tgcagctgct aaacccaatg tataagggct
      781 tgccattgga ttattaagca aagtttgaat ttcagctccg ccaacaccaa gtgctgctcc
      841 aaccaccaat gccattattg ccattggtaa gcgtagctca tagacaatca ctttggtggt
      901 ttcttcaatt tcgcctaatt ggaataaggc tttccaaact tcattaaacg gaagcaggga
      961 agggcctgtc ataatatcga aaatgaggct gagtaaacaa atgagtaaga aaaagagtaa
     1021 taaaaaactg cgtttctttt caatagttcg gtgcttttta gctatttgtt ggggagagag
     1081 cgttgtttgc ataaaaatgt aatgtttgag aattattatc agacaggata agagaaaaga
     1141 tcgataaatt caagaatatc caagataccg actaaagtat tcaactaagt tctcgagtag
     1201 ccctgtcttt tacagtaaaa aactcctgaa tagcggcttc ttctaaaaga gtataattct
     1261 gccaaatttc ggttacagca tttcttaaaa tctcaccttt cttaactctt gatgacattc
     1321 ccaagaatac ctgttctaag aaatctaaat cggcataatg agttaatgct tttcgtgact
     1381 gtaaccagtt aaacatcggc acaaattgtt caggaaaaat cgtgcgttgt tgttcgattt
     1441 gctgaaagat ctgtgcttgt acctcattta aatctttagc aaataaccgt tggaaatgtt
     1501 tagcgagaaa atggtcgatc ataatatccg acacaatgcc tttataacgc ccgaatgtgc
     1561 cttggagtaa ttgggttgtg aggctgtccg gtcggtccga aatcttatca atcgagcggt
     1621 gtaacaccac accgcttttt aagttatccg ctaaattgag atgcggaatt aaacctttat
     1681 agaaatcacc ggcaaagttg ccatataggg tctgttgctt ggtttgggtg tcaatttcaa
     1741 gtgaaatcag cgaatgggta agaaaattca tttatgctcc aagtgcagtt tggtgatact
     1801 caaacccaaa ctcgttggtt tcgcctttcg gtacaaactc aaataaatgc gtaatgcaat
     1861 ttggagcatc gctgtcttgg tgcgaaacaa aaagtagttg cgtttggctg ttagtcacta
     1921 attgttcaat gaactgtttg actaatttgc ggttaatgcc gtctaaacct tgtaacggtt
     1981 cgtctagaat cagaattgat gggtgtttca ccattgctcg ggtaatcaaa agtaagcgtt
     2041 gttgccccca cgagagagag cgaaacggct ttttggcaac attgcttaaa ttgaggcgtt
     2101 ccaaccattc cattgctttt aattgcaagg cattcggcac ttgttgatac acgccgatgc
     2161 tgtcaaaaaa gccagaaaga atgacctcca ttgcggagca atttacccga taatccatat
     2221 gcaactggct gctgacatag ccgatatttt tcttaatatc ccaaatggtt tcgccacttc
     2281 cccgttgttt gccaaatagt cggactttat tggcataaga ttgtgggtga tcgccggtga
     2341 taatcgaaag caaggtcgat ttcccagctc cattcgggcc tttaatccac cagtgttgtt
     2401 taggctgaac cgtccaactt aggttatcta aaatcttctt ctccccatat tgaatattca
     2461 cattttccaa ctcaaacgga ttgatgtttt ccggcaggct gagtagcgga gctgcactat
     2521 caggcagtgg cacattgaca ttctcttctg caaattttag ctgagaatag accgcttgtt
     2581 gttcaatttc tgctcgctct ccttgcagaa ttaatgataa attatctaat aactcaatat
     2641 gattggcagc atcaggaata tcgttaaaac gatttacaat cagcactaac gccattttgc
     2701 tttgtagttc ggcaatcagt tgttgccaat attgcaccga ctgctgatcc agcccttcaa
     2761 acggttcgtc taaaatgaat aaatccggct cgctcactaa catttggcaa aatagcactt
     2821 tgcggctttc accggtggaa aggtgcataa acgggcgttc aagcagatgt tgaatatgca
     2881 atttgtgggc atattcctca cataacgcct cttttggact gccatttaaa attacttgtc
     2941 gtgcggttaa gccgaaatca tcgggcgaga cactatcgtt gttccgatct ttgaaaatct
     3001 gctccaacat tttttgttgt tgctcgaaag agaggagctg aatatgttgg aacgaattac
     3061 catagtcgcc ggaatagagc gagattttac cctgtaaagc gagtgaaaaa gttgttttgc
     3121 ctgaaccgtt tccgtctacg accacctaaa agtcattggg ttggatctgt aaggcgtcaa
     3181 tcgtgagttt atgttgttta accagtgaaa atcgggcatt ggttattgta agtgaaggct
     3241 gtgtttgcat atatttcccg ttttaactta gttgtgtccc gctgacgaac caatccgctc
     3301 gaccgtttaa gaaatcttgc accgacattg gttttttacc cgccggttga agttgggtaa
     3361 tattcagcac gccctgttgg gtggcaattt gaatcccggt tttatccaca ttaattaccg
     3421 tgccggctgc tttttcttgg tgaggtaaca ccgtggcttg gtgaattttg atactttgct
     3481 caacgccatc aacggttaag gttaaaaaac taaacggcca agggttaaaa gctcggatat
     3541 tgcgttccag ttgaactgct gggagttgcc aatccaattt agcttcttct ttggaaagtt
     3601 tctcggcgta attggacagt gcttcatctt gtttttccgc tttgaatgtg tggttggcta
     3661 aaccgtttaa cacctcaagc aatgccagag gggcaagttc tgccagtttg gcatacaagg
     3721 aggcggaggt ttcattcggc tcaatcgggg ttgtgacttt atgtaacata tcaccggtgt
     3781 ctaagccaac gtccattagc ataatggtta cgccggtttc tgtatcacct gcccaaatcg
     3841 aacgctgaat cggagctgct ccacgccagc gaggtagtag cgaaccgtgt acgtttaggc
     3901 aaccatattt aggagcattc aatactgctt caggtagaat taagccgtaa gctaccacca
     3961 ccatcacatc ggcatttagg gctttcagtt cagcttgagc ctcttcctta cgcagggatt
     4021 tgggttggta aacaggaatg ttgtgagctt cggccagctg ttttaccggg ctggcttgca
     4081 gttttttgcc tcgcccggca ggtttatccg gctgagtata aaccgcaatc acgttatgct
     4141 cggaatctaa taaggcttgt aaatgttgag cggcaaaatc aggcgtgccg gcaaaaataa
     4201 tattgagttt tgacatattt tttattcgtt caagttatca atgaaagact tattaaacca
     4261 ttaaacagca caaataaaaa gtggcttgaa tatggtatca ttgccacatt caagataaga
     4321 gaatcaaaca atggaatttt ttatccccaa agcggataca atttttgaag aagagattaa
     4381 aaaaagccga tttatcacct accttcgcca tactgagggt atggagcagg ccaaggcatt
     4441 ttggcaggag ctgaaaattc agcatccgca cgcacgccat tggtgttggg caacggtggc
     4501 aggcttgccg aatgattcgc aacaatacgg cttttcggat gatggagaac cgtctggtac
     4561 agcaggtaag ccaatgctca attatttgct tgggtcaggc ttgggggaaa ttacagcggt
     4621 ggtggttcgc tactacggtg gtatccagct tggcaccggc ggcttggtga aagcctatgg
     4681 caatggggtg caacaggcgt tattgcagtt ggagcggatt cgcaaagttt tgcgaaaaaa
     4741 ctaccgcttg caatgtgaat atgagcagtt taataccatt tctcatctca ttagcgagaa
     4801 agatattgtg attgtcgagc aactttttag cgataaagtg gatttgttgc tggcatttaa
     4861 cccgagcgag gttgaggcct ttgcagatga attaacccaa cgcttttcgg gcaagctcgc
     4921 attaataaaa gaagaaatct agtctatgca atttttttcc attattcgta ttgttggcat
     4981 tttggtgatg tgtttttcat tcacaatgct tgtgccggca acggtcgccc taatttatgg
     5041 cgatggcggt gggcgggcgt ttttagaagc cttcgctctg aattttatgg tcggtacgct
     5101 gctttggtgg ctgtgccgtg acaacaaaaa cgagctacgt tcccgtgagg gctttttgat
     5161 tgtagtactg ttttgggtgg tgctgggttt gctcggggct gtgcctttta ttattttgga
     5221 aaaccccgat ttaaacgtgg cggaatccat ttttgaatcc ttttccggct taactacaac
     5281 gggtgcaacc gtcatcaccg gcttagatag cttgcccaaa gcgattttat tctaccgcca
     5341 atttttgcaa tggctagggg gaatggggat cattgtgtta gcggttgcga ttattccgct
     5401 gttaggcatt ggtggaatgc aactttatcg tgcggaaatg cccggaccgc tcaaagagca
     5461 gaaaatgcga cctcgcattg cggaaacggc aaaagcgttg tggctgattt atctcagcct
     5521 gacgattctg tgtgcggtcg cattttggct cgccggaatg acggtgtttg atgccatttc
     5581 gcatagcttt tccacggttt cgattggcgg attttctacc cacgatgcca gcatcggcta
     5641 ttttaataat gcggcaatta gctatattac cgtgtttttc ctgctacttt ctgcctgcaa
     5701 ctttgcattg cattttgcgg tgattgatcg catacgagat gcacaatcac gcaaaaaagg
     5761 gggtatttta agccgcttgt attggggcga ttacgagttt cgttttttct tgatggtgca
     5821 gttagggctt ttcttggttt gtaccttggt gttagtgggt tacggttatt tcgataatac
     5881 ctctgttacg attgagcaag cgttgtttca atcggtgtcc atttccacca cggccggctt
     5941 tactaccaat gattttagcc aatggagttc gtttttaccc acgctgttgg tgctgtcttc
     6001 atttatcggt ggttgtgccg gctcaaccgg tggcggcttg aaagtgattc gggtgttgct
     6061 gctttattta caaagtaaac gtgaaatcca ccgctacatt caccccaatg tgattcaacc
     6121 gattaaatta ggcaaccacg ttttatcgga acgggtggtt gatggtattt gggcgttttt
     6181 ctcggcgtac tttttcgtgt tcgtgatctg ctggcttgct accatcgctt gtggaatgga
     6241 aacctttgat gctctaaatg cggtaattgc ctcactaaat aatctaggtc ctgcattagg
     6301 gagcgtgagc agcaatttca cccaagtgcc ggacagtgcc aagttagtgc tgacttttgc
     6361 aatggtatgc ggacgtctgg aagtgtttac cttgctggtg attttaagcc ctgttttttg
     6421 gaaagattaa atgaaaacct taattctcta ttttacgacc gatggtcaaa ccaaaaaaat
     6481 tgccgaacga ttggcggacg tgattactca cgaagtcgag ctgatttcgc taaaagatca
     6541 agcggtcgat tttgccgaaa aaattgcaaa tgccgaccaa attgtcatcg gtgcttcaat
     6601 tcgctatggg cattttaacc cgttggttta tcagtttgtg gaacagcatt atccggcatt
     6661 aaacgccaag aaaactgcct tttacggtgt gaatttgacc gctcgcaaag ataatcgaaa
     6721 cacgcctgaa accaatactt atgtgcgtaa atttttagct aaagtgaaat ggaaaccaac
     6781 ccacattgag gtaattgctg gggcattact ttacctacgc tataagccat tcgaccgatt
     6841 gatgattcgc tttattatga aaattaccaa aggcgaaacc gatacctcca aagaatatga
     6901 gtacaccgat tggcagcagg tggagaattt tggcaaatta ttagccaatc agttataatc
     6961 aaactgatct aaatcaaaaa aatgtaaatt tatttaaatg ttgtagaaat tttaaagatt
     7021 ttgattataa tcgccagccc acgttcttgc gaagaccgtg tttaatcatt gaatgaaagg
     7081 aaagccccct tgagtatttt aaaagagttc cgtgaatttg cggtaaaagg taatgttgtt
     7141 gatatggctg tcggtgtgat tatcggcggt gcattcggta aaattgtttc ttcattagtt
     7201 agcgatgttg taatgccacc tatcggttgg ttaatcggtg gcgtggattt caaagattta
     7261 gccattgaaa tcgccccggc aaaagaaggt gcggaagcag tgatgttaaa atacggtgca
     7321 tttatccaaa acgtatttga tttcttaatt atcgcacttg cggtattcgg tatggtgaaa
     7381 gtgattaact cactgaaaaa agctccagtt gtagaagaag ctcctgcaga gccaacagcg
     7441 gaagagaaat tattaactga aatccgtgat ttattgaaaa aataattaac tgattttgaa
     7501 acaagggcta ttttagaata tctagaatag ccctttgtgt tatggttaaa aagaaaaaaa
     7561 ccaacataaa gtcggttttt ttcatatctg ttcgatatta cgctaatttt ttgatttgcg
     7621 ctgctaattt agatttgtgg ttagccgctt tgttagcgtg gattaagttt ttagatgcca
     7681 tacggtctac aactttttgc atttcaacga aagccgtttg agctgctgct ttatcgcctg
     7741 ctgctactgc tgcatatact tttttgatga atgtacgcat cattgagcgt tggcttgcgt
     7801 tgtgttggcg gcgtttttca gattgaaccg cacgtttttt tgctgacttg atattagcca
     7861 aggtcaaact cctaaaatat ctgtgtaaaa attagatata aacaaaatcg ttgtttatat
     7921 tgcctatcga tagtttttaa ttcattttca acgagacacg tgcttgaaaa taacaccgtt
     7981 tttctgatag aaaacgatag ggcgagattt taacagtttt tttctgattt tcctataaca
     8041 aactgctaaa attatgatcg atttttagga tcagactaca agcggtctga tttttcaata
     8101 attttactaa tattactctt tattatgagc aaaaaacttt taagatcagg aatgatcgta
     8161 agctttatga ccttaatttc tcgcatactc ggtttgattc gagacattgt ggtggcaacc
     8221 attttaggca caggcgtgag tgccgatatt ttcctgtttg ccaaccgtat tcccaatttt
     8281 cttcgccgtt tgtttgccga aggggcattt tctaaagcct ttgtgccggt gttggcggaa
     8341 tataatgcgg ataacgatcc aaacaaaacg agagaattta ttgccaaagt atcgggtaca
     8401 ttaggaggtt tggtaactgt tgttaccctt gtggcaatga ttgcctcgcc tgtggttgcc
     8461 gctctttttg gtacaggttg gtttttagat tggctttacg acgggccaaa tgccgaaaaa
     8521 ttcacccaag cctctttttt gctcaaaatc actttccctt atttgtggtt tattaccttt
     8581 gtggcgttat ccggtgcggt gttaaatacc atcggtaagt ttggggtgat ggcattttcc
     8641 ccggtgttgc tgaatgtggc gattatcgcg gtagcgttat ggggcagaga ttatttcgat
     8701 tccccggata ttgccttagc gtggggggta tttttaggcg gcttattgca atttttattc
     8761 caaattccat ttatgaaaaa agagggctta ctggttaagc caaaatgggc gtggaaagac
     8821 gaaggtgtca ctaaagtccg taaattaatg attccggctt tatttggcgt ttccgttacc
     8881 cagctaaatt tactgctaaa ccaagtgatt gcctcgtttt tgattaccgg ctcgattagt
     8941 tggatgtatt atgccgaccg cttaattgaa tttccgctcg gtttatttgg aattgcgatt
     9001 tccaccgtcg tgctgccaag cctttcccgt attgcgaaaa acaaagaact cactgaaatc
     9061 caacgtggag aacactttca aaacacaatg gattgggggg tgagaatggt gctgttgctc
     9121 ggcattccgg caatgatcgg tatggcagtg ctggctcagc cgattattat gacaatgttt
     9181 atgcggggta aatttggttt tgaagatgtg ctggcaacct cttatccact ttgggtaatg
     9241 tgcttggggt taaacagcta tatgttgatt agcgtgctgg caaacggttt ttatgccaac
     9301 caaaacacca aaacaccggt aaaagttggg attattgccg ccttaagtaa tatttgcttt
     9361 ggcttggcgt ttgctccgtt tttgggatac attggtttag cgttagcttc tgcgtgttcg
     9421 gcattagtga atgtgagctt gctgtatgtg aatctctcaa aaaatggtta ctataaagta
     9481 agccataaaa ccgtgctttt tgtgttgaaa ctattcatcg ccgcttgtgt aatgggggcg
     9541 ttggttgcct atttcacgcc tgagattgaa ctttgggcat taatgacaat gtggcagaaa
     9601 gtgtattggc tgatttggct gatgatctta gccggcggta gttattttgc ctctattttg
     9661 gtattaggca ttcgtaaaaa agatttccgc tcggcataaa atgaaatcat taaaactgaa
     9721 aatcccgccg ccggcttggt tcttgctttg tgccgcttta atgtggctgg tagctcaata
     9781 tgtgccggtg agcttgccga attatcagca tacagccata ttttttagcg gtttagctgt
     9841 ggcgataatt ggcggagcgg ttgccgtctc ggcactttgg gtcatacacc aagcgaaaac
     9901 gacatacagt ccttttcgac cacaacatac cgctaaattg gtcacttggg gcatttaccg
     9961 caaaagccga aacccaatgt atctttcgtt actattaggt ttgattgcgt gggcattatg
    10021 gttgggggca atgttggcgt ggctggtttt accgctgttt gtatggttga taacctattt
    10081 ccaaatcaag ccggaagagc aaattttagc ccaaaaattc ggcacacatt atgccaaata
    10141 ttgcaaccaa gtaaggcgtt ggctttaaat gcaagcggtg agattttgtt ggaaatttgc
    10201 aaatttttcc gaaaatctca ccgcttgtgg ggttagtgat tgaaatcagc cgtcattaag
    10261 tcgtgcgagt tgtccatcgc taaattgata aagcgttcat actgttgcag cacgtcggca
    10321 atcaactctt ctttggtcat atattgcaca tcataacctt tccgcccgtc taagaaataa
    10381 gtaatcggct cgtaaatggt attttgctca atgttcggta ggttgtcgtc acctaccacc
    10441 aaatccgata attcccgtgg cacactttca atgccgtaaa tgaaattgcg taaattttct
    10501 ttcaccactt caaactctaa tttcgggttt tgttcgttgg tgaaattcat tttagcgatt
    10561 aagccatagc tttcaaattc ctcgataagt tctaaaaatg ctggtctggc aactttaata
    10621 aagaagtgac ggacatcttg cttattgctt gggttgatga ttttctccaa gcgttgtttc
    10681 caatctttac ccgcccaatg ttggctacct tgcgagaatt ttttgttgaa gtattggtta
    10741 tctaccatta atcctttcca taagccgata cagagaatca gcataatgaa ggtaaatggt
    10801 aaggcgataa tcagtgtcat tgtttgcaga gaagccaagc cacccgaata gagtagtgat
    10861 aacgctaaga tggctaataa tgctccccaa agcacgcttt gccatttcgg tgcattttct
    10921 tcgccttgtg aagcaatgct gttgagaaca aaaatccccg agtcggcaga ggtgataaag
    10981 aaaatactga taacaatgac ggcaacgaaa gaggcaatag agccaaacgg taattgctca
    11041 aaaaaggtaa ataacagttg ttcggtattg ccgctgactt cagctaaagc accggcagtt
    11101 tgttgatcga accaaatggc agagctgccg aagctggtca tccataaaat gttaaacagc
    11161 gatggaacaa atagcacccc taaaataaat tcacgaatgg ttctgccttt agagatcttg
    11221 gcaataaaca aaccaacaaa tggcgcccaa gatgcccacc atgcccaata aaggaccgtc
    11281 cagccgctta gccagccttg atgttccggt tcataagcga aggtacggaa actcatctca
    11341 agcagcgagc taaaataata gcctaaattt tcggtgaagc ttgagaaaag taatagagtt
    11401 gggccggtga ttatcacaaa aataagtagc aatccggcta atgtgagatt gatttcactt
    11461 aagattttaa cccctttgcc tacgcccgaa acggcagata acacggcaag agccattgct
    11521 actgttacga taatcgcaag aacagtaaaa ttgctctgtt caattaaacc tagattgtta
    11581 aagccagcac tgacctgcat caccccaaag ccaagtgtag tcgttaaacc gaaaatcgtg
    11641 ctacaaaggg caataatatc aattaaatgc ccccagaagc ctgaaatacg atgttttagt
    11701 aagggataaa atccggaacg aatagtgagt gctaacttat atctgaagcc aaaataagca
    11761 agagctaagg caatcacacc ataaattgcc caagcgtgaa taccccaatg atagaacgtt
    11821 gtcatcatcg cttctttcat acgctccggc tcagttaaat tttgttggac gggtttaagg
    11881 taatgcaaaa taggttctgc taccccaaaa tacattaacc cgatccccat tccggcggcg
    11941 aataacatcg caatccaaga gccaaaacca aattcaggct catcggtatc tgcccctaat
    12001 ttaatatcac ccaatcggct gaaagagaga aaaattagaa acaggaaaaa gatagagcct
    12061 gcaaaaatat agaaccagct aaaatgttgg aagagactat ttttgatggt atcaagtgag
    12121 gtttgtgctt gctgtggaaa gatgatacag aaaacggcaa caaagctgac aaagagtaaa
    12181 ctcggtagga aaatcggtac cctaaaggta cttcctgtgt ttaatttttt gattaaatcc
    12241 aatgctattt cctctatttt tgaatggtta gaggcttgaa tatagcaaaa agatataaaa
    12301 ataacaaatt taagacgtat tggtaataat attgcaacct aatgggtttg gtaaaaaaat
    12361 cgggaaattt gaccgcttgc acattcatta tttgcgagta gaatattttt catttataaa
    12421 aaacgaataa agaatgcaca cacaacataa cgctcaaaaa atggttttgc ccattttact
    12481 gctgatttcc ctttctcatt tgttgaatga tttagttcaa gcagtaatgg tttcgatcta
    12541 tccaatgcta aaagaaaatt accaactgag ttttgctcaa attggtatga tttcactcat
    12601 ttatcaaatt accgcatcta ttattcagcc gggtattggg ctttataccg atacccaaaa
    12661 ccgaatttat tgcccgtcag tatgctgatt acgcttcttt ccgtgatttt acttgccttt
    12721 tcgccgaatt ttggcgtgtt actgatagcc gctgcgttaa tggggatagg ctctgccatt
    12781 tttcaccctg aagcctctcg ggtggctcga atggcatcgg gcggtaaatt tggtacagct
    12841 cagtcgcttt tccaagtagg tggaaacagt ggatcggcgt taggtccgct cttagttgca
    12901 ttatttattg tgccgttagg gcaaagtgcg gtgatttggg ttgttgcatt tgtattattg
    12961 gcattatgat gggttttata taaagtaagc cgttgggtga aatatgaagc caaaccgctc
    13021 attatttcac caaaccatat tcaacaacgc ttacacggta aattattagc cagagcatta
    13081 ttaattatcg ctttattgat gttagcgaaa tttatttata ttgcgagcct cagtaactat
    13141 ttcacctttt atttaatgga aaaattccaa ctcaatatag caatggcaca gctctctcta
    13201 tttacctttt taggtgcagt tgctttaggc gcttttgtcg gcgggccgat tggcgataag
    13261 attggacgaa atgcggtgat ttggttctct ttcttaggta tggcaccttt tgctttgttg
    13321 ttgcctcacg cagatctctt ttggacgatt gtgtgtgcga tttttgccgg ttttattatg
    13381 tcatcagcgt tctccgctat ggtggtttat gctcaagaag ccgtaccagg cagagtaggg
    13441 ctgatttccg gcacaatgtt cggtttaatg tttggtattg gcggcttaag tgccgccgta
    13501 ttgggttttt ttgcggatga atatggcttg gagagtattt ttaaagcctg tgcctatttg
    13561 ccactacttg gttttgctgc attagggcta ccaaaacgaa atatttaacg ttttattttt
    13621 agcgtttgac accagctttt ttcataccca ataagaaaac ccccacccca atccaaacca
    13681 gaatgtagcc tacagcacgt tcggcggaaa gtgtctcatt aaaaatcagc acaccgcata
    13741 aaaattgcag ggtaggtgaa atatattgca gcataccgag tagcgacatt gaaatgcgtc
    13801 ttgcacccat tgcaaaccat agcaatggta aggtagtggc aatgccggag cagagtagca
    13861 ctgccatttg taaaggattg agttcactga ataccaaaat gttttggtgg taacaaaagc
    13921 agagataggt gatggcaaaa ggcgacatca gcaaagtttc taacgccaaa ccagacaaag
    13981 gttccattgg ggcaagttta cgaatcagcc cgtaaataca gaaactggcg gctagaatca
    14041 atgcgaccca cggaatttgt ccggcaggaa tagcaagcca taaaatgccg ctaattgcaa
    14101 acagcagagc caataattgg gctttattca gctgctcttt tagaataagc ctaccgacaa
    14161 acacgttgaa aatggggttg ataaaataac ctagactggc atctaaaata tgctcattga
    14221 cgattgccca taagtaaacc agccagttca cgccaatcat aaaagaagaa agcacgaaca
    14281 gcccgagtac ctttggctgt ttaaaggcat taataaccgc tcgcccttgt tggaaaatca
    14341 gcagcagaaa cacagcaaat agggcagacc aaagcacccg ttgagccaaa atttgctcgg
    14401 caggcattcc cgattggttg atcggatacc aataaatcgg aaaggttccc caagttaagt
    14461 agcacagtag ggcatagtgc caaccctgta atttgcctga aatattcatt ttgctttcct
    14521 aaacgagtgt acaagcggtc atatttgtcg atttttttgc aaattgcaaa tcacaaaacg
    14581 gcataaaaaa ttaggctttt tcaatgccga aagaaataac ctcttcaatt ttttccgcat
    14641 ccattgccag cattaacagc ctatcaaccc caagggcgac acctgagcag tttggaaaac
    14701 ccgctttgag tgcggcaaga aaacgcacat ctagttgctg tggctccagc cccatttttt
    14761 cacgttgcag attatcttgc tcgaagcgat gaatttgctc atcagcatcg tctaattcgt
    14821 ggaagccgtt acagagttcc aagcctttgt agtaaaactc aaaacgttcg gcaacacggt
    14881 gatcttccga gctaatttga gctaatgccg cttgggtgga agggaaatga taaacggcag
    14941 ttggtctttc ttgcccgata ttggcttcga caacctcgct gaataaaaat tgtaacaagg
    15001 tgtcacgctg ttcatcatca tcacattgca agccgtgttt tcgggctttt tccacaagtt
    15061 gtgctttggt ggcagaaagc ggatctaatc cgacataggt ttggaacaca aattgatagc
    15121 tgaatgattc tgccggttcg cagtccaaaa tttgttgcag taaatcatcc acttcattga
    15181 ttaagcgata catatcaaaa tgcgggcgat accactccag catcgtaaat tcagggttgt
    15241 gtcgcttacc ggcttcctcg ttgcgaaata ctttacacag ttggaaaatc ggtccactcc
    15301 cagcagcaag tagtcgcttc atatgatatt ccgggctggt catcagatgg agtgttttcg
    15361 cctcagggga aaaaggcgat tgaaactgcg tattgaaagt cgaaagatga acgtctgtca
    15421 ccgaaaattc gcttaaaatc ggggtttcta cctctaaaag ccctcgatcg gtaaagaact
    15481 tgcggatttc cgtaatgatt tttgaacgtt ggatcaggtg aggaatagag gctgttggtt
    15541 gccaatcaat attatcgagt tgtaaattgt tcataaaaaa tgaccgcttg aaaatgtgag
    15601 gctattttag cctaaaaaag tgagttgccc tagttaccga tcaagaaaaa taaagagagg
    15661 gttttaactt ttttgcaaca aattaaattt tctcatttta aacattagaa
//
LOCUS       DANLUA010000040        15641 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLUA010000040 DANLUA010000000
VERSION     DANLUA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXB44_000924"
     CDS             complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXB44_000924"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL5281422.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1067..1828)
                     /locus_tag="PXB44_000925"
     CDS             complement(1067..1828)
                     /locus_tag="PXB44_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL5281423.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGRGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXB44_000926"
     CDS             complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXB44_000926"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL5281424.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXB44_000927"
     CDS             complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXB44_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL5281425.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXB44_000928"
     CDS             complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXB44_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL5281426.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXB44_000929"
     CDS             complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXB44_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL5281427.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(7602..11699)
                     /locus_tag="PXB44_000930"
     CDS             complement(7602..11699)
                     /locus_tag="PXB44_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL5281428.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11780..13615)
                     /locus_tag="PXB44_000931"
     CDS             complement(11780..13615)
                     /locus_tag="PXB44_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL5281429.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     SHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13627..14472)
                     /locus_tag="PXB44_000932"
     CDS             complement(13627..14472)
                     /locus_tag="PXB44_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL5281430.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(14473..15636)
                     /locus_tag="PXB44_000933"
     CDS             complement(14473..15636)
                     /locus_tag="PXB44_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5281431.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
BASE COUNT         4377 a         3517 c         2929 g         4818 t
ORIGIN      
        1 gtaatgtccc ttttttgaat gtctactaca aaatgggata ttttacaaac cgattgacta
       61 atgaagcaag aatataatta actacaactg gatatatttt gcaaatttcc cgataaattt
      121 caccgcttgt agataaaaac cgtagataaa aaccatagat aaaaaatagg agctaaacat
      181 ttttagcccc taccgtcatc agaaataaat cccgactaaa atcgccacga acacacctaa
      241 gccgaaatag tggttattta gaaacgcttt aaagcattcg ctacgttctc tttttttggt
      301 cagccaacat tggtagataa acagcgtggc acttaaggct aatacgacaa aatagccgat
      361 gtgataatct tttaccacgc ccaataaccc caataaaatc aaggtgataa actgtaataa
      421 cgcaataatt ttattatcgt attgagcgaa taaaattgca gtggatttaa ctccaattcg
      481 taaatcatcg tcacgatcaa ccattgcata ttgggtgtcg taggcgactg tccacgctag
      541 attggcaaaa aacagcagcc agcattcaag tggaagagtt tccgttaccg caccaaatgc
      601 catcggaatc gaccaaccga atgccattcc aagcactact tgtggcaagt gggtaaaacg
      661 cttcataaag ggataaatca ccgccagaaa aactgccaca aaggaaagca gaaacgcata
      721 gcggtttagc attaaatcga gcagaaaagc gaacaacagc aagatggcaa aaatgacttt
      781 tgcctcagtg gtggtcactc tgccggtagc taatgggcgt tgagaggtgc gtttaacgtg
      841 tccgtcaatt tctctatcgg cataatcatt aatcacacaa cctgccgcac gcataacgat
      901 taccccaagg gtaaaaatgg ctaaaatcgc aacaggcggc attccctctg cggcaataaa
      961 taacgcccat agtgtggggt gtaataataa taacgtgccg atgggtttat cgaaacgcat
     1021 taactgggcg taggcaatcc atttttgttt agaaaaaaga gtttcgttat tcattacaaa
     1081 ttttgacgat aacgtccgat tggcttaaat gagtctgtaa ttctaacggt aaatcttgat
     1141 ctgtaaacaa ataatttact tctttaacat ttcctaaacg tgcaatagct gtacgggtaa
     1201 actttgaatg atcggtcact aaaaccactt gtcgggaaca ttccattaat gcacgcttta
     1261 cctgcacttc gtggtaatcg taatcgagca tagagccgtc catatccacc gcactaatgc
     1321 ctaaaatccc aaagtccaaa cggaattggt taataaagcg aaccgtttct tctccgatta
     1381 gcccgccatc acttcttagc cctctgcctg ctaccgtaat ttggcaatct tcattttgca
     1441 tcagaatatg agccgcattt aaattattgg taactacttt taaattttta tgcgaaagta
     1501 aggctaatgc cactgcttct gaagtcgtgc caatatctaa aaataatgat gctccatttg
     1561 gaatcagttt tgccacctgt tgggcaatcg catttttctc gtgtgagaaa aattgcttgc
     1621 ggtgggtgta atcactattc tccgtattgg aaggagcggc tgccccaccg tgatgacggc
     1681 gaatcagatt attttctgcc atttcattca gatcacggcg aatggtttgc ggactcacat
     1741 tcaattcagc cactaattgc tcggtactca catagccaag ctgattaacc agctcaatga
     1801 tctttttgtg tctgtttgcc tgtttcatat ttcctctttt gcaaataatt tgcagaaaaa
     1861 gaccgcttgc aagcggtctt ttttcagtta aattttacaa tttatcaaca agttcaaccg
     1921 catagccgat atagcttgcc ggtgtcatct ctttcaaacg atttttttca ttttccggaa
     1981 tttccaactt atcgataaac tcacgcattg cggtttcatc aacccgtttg cctcgagtca
     2041 gctctttaag tttttcatac ggtttttcaa tgccataacg acgcataaca gtttgaattg
     2101 gctctgccaa tacttcccag ttttggttga gttcatcacg caagtgttgt tcattcactt
     2161 ctaatttgct aatacctttt aaggtggacg cataagcaat taacgcataa cctaaaccta
     2221 cacctaaatt acgcaacacg gtggagtcag ttaagtcacg ctgccagcga gaaatcggca
     2281 atttttgccc caaatgagcc attaccgcat ttgccaagcc aagattgcct tcggaatttt
     2341 caaaatcaat cgggttcact ttatgtggca ttgtgctaga gccgatttca ccggcaatag
     2401 tgcgttgttt gaagtgattt aaggcaatgt agccccacat atcacgatca aaatcaatta
     2461 aaatcgtatt aaaacgagcc acgcaatcaa agaactccgc aatataatcg tgcggttcaa
     2521 tttgggtggt gtatgggttc cacgtcacac ctaacgattc cacaaattct tggctgaaag
     2581 tgtgccaatc aatctccgga tatgctgata aatgtgcgtt atagttgccc actgctccat
     2641 tgattttagc gagaatttct aaattttcta actgtttata ttggcgttgt aaacgatatg
     2701 ccacatttgc catctcttta cccattgtgg tcgggcttgc cggctgaccg tgtgtgcgag
     2761 aaagcaacgg aatatgttgg taacatttcg ccagctctgc tacagcatca atcacttttt
     2821 tccattcagg taataatact tcttcacgag cggttttaag cattaaagcg tgcgaagtat
     2881 tattgatatc ttcagaagta caagcaaaat gaataaattc attcaccgct tgtaattcag
     2941 gcaacgcttc acatttctct tttaggaaat actccaccgc ttttacatcg tggttggtgg
     3001 tacgttcaat cgttttaatc cgattggcat cttctaacga aaaatcagcc acaatttggt
     3061 ttaggtaatc gtttgctttt tcagaaaaca ctggaacttc attaatttgt gtatgcgatg
     3121 ccaatttttg taaccagcga acttccaccg tcacacggaa ttttaataag ccaaattcac
     3181 taaaaatcgg acgaaggctc gcaactttgt cttgataacg tccatcaatc ggggataacg
     3241 cagttaaagc ggtaagttcc attgttttat attctccaaa taggtaaatg ttcggttaga
     3301 tttgacgata aaatttctgt gcttggttaa aaattttctt gcgtgaaaat aaaaattgcc
     3361 aacggcttcc gccgacttgc tgccacaaaa taccggctcg aataccggct agtagcgagg
     3421 ctcgaatttt attctgtata tccgggcgaa ccaagtaatc ttgcattccc atcacgtgaa
     3481 ttttcgtacc taacgggctg ataatatcgc tataaatagc ggctaaattg gcaatcattt
     3541 gatctgccat cacattatct tcatacaggg ctaactggcg ttccacttgc tgtaaacgct
     3601 gggctaactg ttgttttgct tcagggtttt tattgagttt ttggctaagg gcgagcaggc
     3661 ttatccaata acgtccgact tcggtatcta atttgccgtt accgccgcca gtttgagcta
     3721 atgcggtttc taagcccatt tttaaatggg aaagatcatc gccaaataca cttaacgttg
     3781 attccggctg agtgattaac aggctttcta aagcacactt aaaagcctct ctgtctgcca
     3841 tgcctttatg tgcaaattgt tgcactaacg tcgccgcttg gcaaacgccg gcataagcaa
     3901 gggtaatatc gtggtaatcc gccatctctg taaaattcaa tatgataaaa cattagtggg
     3961 aatatagcat tttttcagct tcttttgaat ggcaaaaggc atttattcta ttgaaaaata
     4021 aatgcctttt cattaatacg caatttgctg caaaaactat cctaccaatc gcccattatc
     4081 tattcgataa accacatcaa atttatccag tccgctttgg cggtgagtga tcataattaa
     4141 ggtgcgatca cagcagtgag tcaaaattag gtttaaaatc tgctgttcgg tttctctgtc
     4201 cagtccttct gttggctcat caagtaagac taattcagca gaactcagga gcaaacgggc
     4261 aagccctaaa cggcgttgtt cgccaccaga aagcggtctg ccgccctcgc ctaaccataa
     4321 atttaagccg tctgtttcaa gcaaatgccc taagcctact tttcttactg cttcttgcat
     4381 ttgttcgtct gtagcttgag cattccccat taataaatta tcttttaggg tttggctaaa
     4441 aatatgcact cgttggctta aggtaactaa ttttgagcgt aacatcggct ctgaatattc
     4501 gtcaatttta caattattca gccaaatttg accgcttgta gggtcgtagt tgcggtttaa
     4561 cagttggaat atggtcgatt ttccgctacc cgttttacct aagattgcca ctttctgccc
     4621 ttttaatacc ttaaaggaaa gattatttag tacgttttgc tcgccgtaat aagcaaagct
     4681 cacgttttca cagcgtacaa gcggttgatt ttggctcaaa ttttgccatt ctgccttgct
     4741 accaaatgtt acattaggct gttgttcggt aatttcacta atccgctcgg cggcggtaat
     4801 cacttgccct aaatgcaaaa aggcgatacc aatcggggaa agaatctccg ccgatgccat
     4861 tacacagaaa atcactaacg caattaaggc ttcagggtat tttgcagtgg gcacattaat
     4921 cgaagttgaa accaaataga tcaccacaca agtcaaaatg ccgttggata acattatcaa
     4981 gctatttgat aagccggaca gtcggctttc tttgctttga gcgttcagcc attgttgttc
     5041 agtttgctgt agtttttcgc tcatttggtt caagttgcca aagagtaaaa attctgcatt
     5101 taactgtacc cactcaataa attggctgcg gtaatttgct cggctttgta ttgcattacg
     5161 ccctaatttc aaacccaatc gataaaatac ggtaggaata acggcaagca ataccaatag
     5221 tgtgccgcaa atgatcagca ttaatggcac ggaaacaaaa gaaagcccga ttgccataaa
     5281 tgcaataatc atcactgcac tgacaaaagg cgagactaaa ttcagatata gggtgtctaa
     5341 cgtatccaca tctgccacca agcggtttaa caattcgctg tttctaaagc gatttagccc
     5401 gcttggactc agtggaatca attttttaaa gacgctagca cgcaaattcg ccaatacccg
     5461 aaaagtcgca tcgtgggtga ctaaacgttc aaagtaacgt gaaattgtcc gccctactgc
     5521 caaccctctc acccccgatg aagggtagaa aaagttaaaa ataagcgcat tgccggctag
     5581 aaatgaggca gctaaaaacc aaccggaaag gctgagcaaa ccgatacttg ccgctaatcc
     5641 taaaattgcc agcacaacac ctaataatag tcgcccaaaa tgggtttggt agagagcaaa
     5701 aaacgggaac aacgatttca ttagatttct ccctgatgca ctaattcatt atgtattaat
     5761 tccgcaaaaa agccggtgtg ttctaattca gtgaaacgac ctttctgaat gatttgccct
     5821 tgtttcatca cccaaatttc atcgcattgg gttaaatctt ccactcgatg agtaatcatt
     5881 agcgttgttt gctctcggct gagattatgc agagcggcta acacttgctg ttccgaatcc
     5941 atatcaaggc ttgcagtcgg ctcgtctaac agtaataatt cgtacgggcg aagcaacgct
     6001 cgtgcaatcg caatccgctg tgcttgcccg ccagagatac cgatattact gtcttgcact
     6061 tgatgctcca accctaaacg tgccacaaat tcatcggctt ttgaaagctg taatgccttg
     6121 gctaattcag aatctgtcgc attagggttg cctattaaaa tattctcttt caaactgcca
     6181 tgcattaatt gcggattttg ccccacccac gctaatttcg ctcgccattg gcttaaattt
     6241 aaatctcgca gttcggtgcc gttaatcgta accgaccctt cataaggcaa gaaaccaagt
     6301 aacatattca ttaatgaact ttttcctgca ccactttgcc caaccaaagc gatatgttgg
     6361 tgtgcattca actcgaaatt taacgcttgg gttaaggctt tgccttgtgg tgagaagatc
     6421 acgcaatctt tggcttgaat cacaagcggt tgattttcaa cattttcttg caaattgcca
     6481 ccttgcgttt tcaccttttc ttttaagaaa gtttcgatat tatctgcggc actaatcgct
     6541 gctgccttgt cgtgataaaa cacgccaagt tcacgcatcg gttggtaaaa ctcaggtgcc
     6601 accattagac aaaagaagcc gataaataag gtcacgcccg taccataata gccaaaatcc
     6661 agctcaccta aaaaaataaa gccaaaatag accgccgtca ccgcaatcga taccgccgtg
     6721 aaaaactcca ataccgcaga ggataaaaac gccattttta gtacgtccat cgtactgatt
     6781 cggaaatctt cggtgctttg gtagatttgt tcagtctgct tttccgcttg cccaaataag
     6841 cgaatggttt ctaaaccttt taatttatct aagaactgtc cgctaatacg ggaaagaatg
     6901 ccgatattac gctgatttgc ctccactgct ttcattccgg ctaaaatcat aaataaaggt
     6961 aataatggca ttgttgcaaa taaaatagcc cctgctgccc agttaatcgg gaatacaaaa
     7021 cagaggatca gtaatggcac tatcagcgat aaaaattgct gtggcaaata gcgtgcgtag
     7081 aaattatgta gattttccac ctgctcgagc attaaggttg cccagctgcc cgccggtttt
     7141 tgttgaatgc tcatcggccc gactgcttcc attttggcta gaatttggcg gcgaagatgt
     7201 aaacgcaagg tttgccctgc tttgaaaccg acacgctcac gcagaaaaat gaagaatgct
     7261 ctggcagcaa aacaacagaa taagagcata atttcaacca gaaagctatt cggtgactgc
     7321 tttccgataa gcattttgtc taacattgtg gcaatcagcc acatctgtcc aatcatacat
     7381 aaactactga aactccctaa cagtatgttt aagtgcatat attttttgat gactttttgc
     7441 tgacttttaa gccatttttg taattcgcgt tggcgttgtt tttccataat tttgtcaatt
     7501 tttttgtttt ttcaaccgct tgtaaatcgg gtttgccatt ctactataaa gcaaaaaggt
     7561 tgttaatggc gaaacattaa caacctttca ttcagcctta actagaatag ctcgtcaaat
     7621 tcaaagcgat acattaaatc taccgcttgg ttaatacttg agacccattg taagtacaat
     7681 cttggcgtta aattatagcg taaggttaat tcggtaagcg gtgcaaaaat gcccacgcca
     7741 tacttcactc tgaatttagg ggcaaggctg gcacttactt ctactttggt gttatcgcca
     7801 atacctgcgg tggtcacgct taaatctttg agtccgaaag tgctaccaac atcacccacc
     7861 aatttactgc tttttgataa gctcatactg agtaatgccg ctgcgactgc attactggag
     7921 ctggtatcgc cactgctttc caatgaacga ccagttaaaa tataggaaag tgcttcattt
     7981 tgcgacattg ccggatcaga gaatactttt accgttggat tgtctgccaa accggtcact
     8041 cttaccccag ctgtaatggt cggatcttcc attgcctccg gattgcgtat agcttcaata
     8101 tctaaggttg gctgagacgg tgagccggca aaactaatat cacctttacg aatcagcaag
     8161 tcttgaccat aagcggcaaa tcgtccttca cgcaaatgaa cttgcccgta aagccctaaa
     8221 ccttgtttgc cttgcctgac agacaataag ccattcacgt tactgtttaa accataggct
     8281 ttgagtttga catcattacc gatgttaatc ttaatatctg cattaattgc cattccacct
     8341 gaggtttgag ccggaatatt acgttggcta aacggcactt tatttttcac agagccgtcc
     8401 ataatcacct catctccact tacactgacc gcactttcag gtaactgctc gacttcaatt
     8461 cttgcccaag ggatatctac atttccactt aaggttaaaa gagttggcgt tgccgtcact
     8521 tgaatatccg ggctaactgc cacttttgcg atattaggaa tatccacttg gaaacggtta
     8581 gcttgagcgt gaacccgtgt tttccacgca tttagatctc gccagtctgc atcaccctcc
     8641 aaacgcagtt cgctttgttt ggtttgtaat ttaccgctta aagtcgatgt tgccccgtgg
     8701 aaattcattg ctaaattccc acctgtaata tcaaacggca ttgtaacaga gcgagctttt
     8761 aactctgtaa cgtttagatt tccgtgcaat aacggagagg ttgcggtgcc gctcacggta
     8821 agacgagcat tcacattgcc atcaaccgaa tcgccccgtt caagtagcgg tcgaatcagc
     8881 tttaagctta attggtcaag atgaatattg ccggataacg cacgagcctt acttaaatct
     8941 ttcatcacta aatcagtcga aagtctgccg ttattttcga cccttaaatc agatttaagg
     9001 gttaaattat tgtcagacat tttcagatca attttaagcg gtgaaacggt gagtgggaaa
     9061 ctttttcccc cctccatttt ttgaataaat ttaatcgcat tagaggttaa atccagattc
     9121 acttgaggct gtttgttttt aaaccaagct gcatcgcctt tggcattcac aatccctgaa
     9181 agctggctgt ttttctccaa aaattgttgc aataccgcta aatcaaagtt acggatttca
     9241 aaaggcactt taccctcaac accggcatta aatgcagtcg ggaaacaaag attaattttc
     9301 cggttatgcc aacaatgtgc ggaaatattg gcgttaatgg ctttattgtc atatttcaca
     9361 ttcactgctt tatctgcttg gaattgacca aactcggtgg attgaatcga aacttggctt
     9421 aattgccctt cccaaatttc tttcaaacgg tcaaattttc cagaaatttg caaatcagca
     9481 ccaaccggat tgccttttgc acttaatttc aagctatgat tttcttcatt tccttttagc
     9541 aataaagtgg catttcccac tttgatttca ttataggcaa attgacgtaa ccccaaggtt
     9601 aaatcccctt gaatggtttt ctcagtggta attttgcctt ttgcggttaa attttgcagt
     9661 ttaaattgct catagcctac atttgaggcc actaaatcta aatcaatgtt aggttctgaa
     9721 accttacccg ataatttggc attaccattc acgctggctt ttaaattcgg cactaaccct
     9781 tgcaaattag gggctttaat atccgcatag aaatcagatt tttcgcctaa cacgcctttt
     9841 aaatcgatct tattttcacc atacaccagc gataagcccg gaacatctaa aagttgttga
     9901 tgatcggctt ttaaattacc gcttaagtgt agatttttct gtaataacga acctttaata
     9961 tcaatattcg agacgtccgc tttccatccg ctagctgaat tgccccgtcc tgcataacct
    10021 tcggaagata aagaaccgga caataccgcc ggccagtctg ccagtaggga tttagtgtta
    10081 atatgctgaa gtttcaaatt tgaacgccac gcaacgccct cgctccaatc cactaaaccg
    10141 tcgatcttcg ccgtaccttc cagcgtattg agacttaaat cttgtatatt aaaatgggtt
    10201 aattcccctt tgccagttaa actagccgag cttgcaggca cgcccattcc tttggcactg
    10261 actttagcct ccaaagcgta atccaggaga ttgccattaa gcctcatggc gatattctct
    10321 aaactcagca catcaccatt ttgctgagga gcaaacggat aacgcattga ctcactcttc
    10381 agcgataaat caaagggagt tttcgcctga gccagtgcaa cattgccctc caacgaggcg
    10441 ctaattgcac cagccgtctt cacttttagg ctggttttgt catatagctc accggaaaga
    10501 gaggcttcaa tttgattgaa cggcaatttt actttcgccg tttttgcctc gtgaccactt
    10561 agcgtccaac ttagcggata attatcttgt aacgataaag aaccgcttgc ggttagatcc
    10621 ccttggtcgc ttttgaaatc taaacttttt aggttgatat gctgatcttt tgcttgagcc
    10681 tgaagctgta aagaggcaac atttaacaat gagatcggct caatcaaatt ccctttttca
    10741 tctttagctt tttgagcaat cgctacattc gcaatatcaa tctgttcaat attgagatca
    10801 agaggcaaac taaagcgaat atttttatcc aataacggct gtgctaattg tgctttaatc
    10861 gctgcccaat ccactttatt atccacctgt tcggcctgtg caacagtttt tctctgtaat
    10921 tggtgaaagc cctctacgac tttttcactg gttttttcca ccgcctcttg aatcacttta
    10981 tcagcaatct cttgtggatt tgtcgctgat aacgcctctg tttttgcatt tttttgtgca
    11041 aaatcgaccg cttgttcagt atcagtttcg tctaacttta aggctaaaag cagatttagc
    11101 ccctctaact gtgtcgggtg aacggtaata gcccgccctt taccagaaag tgcagtgtgg
    11161 aaatgattaa gatgaatatc catatcatca actttgacct tgatattctc taaattgata
    11221 ttttgagctg aaatccccaa tggcaaattt aattcggtga aaggttcggt ctcttctttt
    11281 ggctgagaag ggggtaattt agtcgtatca atcaacacct ctgtatcttt aaggctgaga
    11341 ttattcaaac acatttcata gtggagcaag cagctaaaac caatatgcag atctgcttgc
    11401 ccaaccgtta cattcacacc gtccatcaca aatcgggcat cagaaagctg taacccatct
    11461 tgcaggctac cttgcacttg cccaagttgt aatggctcta cgaatttatc tacccagctt
    11521 aacactttac gttgcccgta acctgtgcat agaaagagaa tggcgagtaa aagtagaata
    11581 aacgtcagac aaaagccgtg cttcaaaaac cgccactttg attttttagg ctgtgttgct
    11641 tgtggctctt gtgctgagtc cgtttgattt gtggctgttt gtttattttc gtcagtcata
    11701 atcgtgcata tttatgaggt gataaaaagt aaacaagcgg tatgcgaaat tttactgaaa
    11761 ttcgaccgct tgttttcatt tacagatcgg aacctaaacc aatataaatt tgtattcctt
    11821 tggaattatc tggtgatttt accggcgtgg cgacatcaaa tttaatcgcc ccgataggtg
    11881 atgcccaacg cacccctaag cccgtaccgt agtagagatc ctgagttttg aatttattcg
    11941 atgccaagcc tgcatcataa aaaattgcac cccaccaatt agggtaaacc tgatactgat
    12001 attctactgt gcctgtggct aaatgagagc ctccgacacg tttgccatta ctcggatcac
    12061 ggggggaaat atccttatag ccaaagcccc gcacactcat atcgccaccg gcaaaatagc
    12121 gtaacgccgg tggaatacgt tcaaattgat tggctttcaa ataccccaac tccgccctta
    12181 aatagaaccg atggttatca aagtaagttc tcgcccaaga tgtcgaggct ttataactat
    12241 aaaagtttac atctgaacct actgcacgac tcccccagtt taccgttaat ttttgtgtat
    12301 ccccccataa tgggtaaggc ttgccatctg ttctggttcg attcaatgat gccgttgggt
    12361 aaactaataa ggttttaaat cgatcattag cttgagtaaa agaatcgtaa cgggctttta
    12421 aaccaacaga aaatgaccaa ccggtttcgt gagtccagaa acgttgtaag ccaagggtgg
    12481 cagcggttga ttttgtatcg ttttgatctt cgttttctaa agtgccggat aattggtaat
    12541 aataatgtag cggatcttct ctaagtggaa tattataacc aaattcaaat gtctgctccg
    12601 gcttagaaac gtacgttcgg ctttcaatac tatgacctcg gctatttaac caaggtttct
    12661 tccagttaag ctgtaacctt gggccaactt cagtttcgta gcctatccca atttccactt
    12721 cattgcgttt tttaggctga aataacacat ttaaatccac taatttatcc tgttcattta
    12781 aatgtgactc aaccatcact gatgaaaacc aattactgga aaggtaatct tgggttaaaa
    12841 tcgaaagatc gttagctaaa tatggctcac cggaatgaat tttaagaata ttttgcaaat
    12901 attcttcttt gatttggcta tcaacaaacc ggatttgtcc ataatggtat cgttttccgc
    12961 tatcatagcc taaacgccaa tccgctttat gatcggtcgg ataaacttcc aaacggtgat
    13021 aaagccaatg accgtcaaaa taccctttac taaaagcggt actttcaata tcacctttaa
    13081 aattatcata ggttttatgg tttaaacgac taccttgttt tggggcttta ctgactagat
    13141 gagcgaaatc ttcgtccgtt tttgcatcgc cttgcaaaac aatatcggtt tcatcaattt
    13201 ttaccgtctc tttatctagc tcaacattta atactaagag cggtttcttt ccctcttgag
    13261 gtagctgcac aaattggtat cggctattat aatagcccaa ggctctcaga gctttatcta
    13321 ctgtggtttg caccaaatat tgataacgct ctgaaccatc tgcatattca ttggcaattt
    13381 gatctaaata tagctccaca tttttcttgg catctttacc ctgaatgcct ttgacttcta
    13441 aagtgactaa tggctctatt tctggctcat catcactatc ttccgctaat ttttcttgca
    13501 gattttcaac tcgctcacga tctacgtcag acagcctttg ctcttgattt gcttgtgcca
    13561 ttgaagcaaa agccactaaa cagcagaatg ttaaaaaatg tagttttgta ttcattagta
    13621 acctttttat tcttttggaa tgccacgcaa aagcggaata ctgtcataag gcggaacaga
    13681 acttaacgct ggctcttgct caaagagcat aatgaatggt ttgtcaggca ctgttttttc
    13741 attacgccac aagtttccaa cactcaccaa ttcaatatct tgaggtagtt gtgctaaatt
    13801 ttgcttcagt acactaatcg tattcttgcg agggtgtcct atcaccaccg ctaccccgtt
    13861 ttttcgtgcg tgattaattg catgtgcaaa ctgtttttgt acatcagcaa gcagatcgct
    13921 atcatctaaa aaaatgtctc tctccagcgt attaatacca aattgacgag ccgttttagc
    13981 cgccacacta ctgccaatag ttttactgtc taaaaagaaa agcgtatttt cttttaacac
    14041 tttcaacaaa tgttgcattg tagcagagtc tgaagttgct ccactgccca tatggttatt
    14101 taaaccaata gcatatggta cttgaccacg agaagtatga ataagtttcc gaattttctc
    14161 ttcgttatca ccaatatgaa tacccccctc ttcaattggc tgtttcgatt taggttccat
    14221 tggcaaatga atcaaaatat cccgtttttg gttataagcg tcctttgccc gttctgttgc
    14281 atagggtgca acaggaataa tcgccacgct taccgccttg ggtaaggcta agatttctcg
    14341 atcttctttt attcgatagc ccacatcatc aattacaatc gccatttgag ctgcttgtac
    14401 aagcggtgta aaaagcacaa acatttgcaa aaaaatgtta aaattcaacc gcttgttaat
    14461 ttgtttaaac atctatttca ccaaatttag aggattaatc ggcgtgcctt tacgggtaac
    14521 accaaaatag agtgcagaac ggctttgtcc cccactgtta ccaaccttcg caataacctg
    14581 tccgccttgc actcggctac ccttgctgac tgaaatagat tggttatagc cataaaggga
    14641 aatatcacca ttgccgtggt caatcgcaac catattgcca taccctgcaa gccattgagc
    14701 cattatgact cgcccgctcg ccattgctct aaccgaagta ccggcagaag caccaatcac
    14761 tacaccgtgc caattattac caaaaggagt gataatattg ccagacaccg gcatcgtata
    14821 tcgaccagcc ttgatcggtg ttctctcaac attctcagtt gttttggact gaccggattt
    14881 tgcctgtttt tcccgctgtt gttcacgttt ttctgcttgc tgagcctctc tttgcgcttg
    14941 ttctaattgt tgttttagtg cctgttcatt gctgcgtaaa ttctctaatc ggctttgatc
    15001 ctgctcaagc gttttatcga ttaaacgtaa ggtactttca cgctggcttt gtactttctt
    15061 taaatctttc tcttgtttct tttgttcatt aagttgcgtt tgatgatctt tttgctggcc
    15121 tttcaattca tcacgtctgg ctttaagatc agcttgggtt ttacgaattt caacgatcgc
    15181 atcaatgcga atattattca tatgctgata ataagcggtc attctttccg cattttttgc
    15241 ctcttcagac attaaacgct ctaagaccga agggttcacc ccagaacggt aggcagagtc
    15301 caactgctct tttaattttt ctttctgctg tttttccaac ttttctaaac gggcaatttc
    15361 agtctcagtc gctttaatgg tctggcgaac ttccgttaaa gacatttcgg ttttttgtaa
    15421 tttatccaac accttcccca tttctacttc ttggtttttt agggtagatt gcaacgcatt
    15481 acgtttttgc ttttgttcat taatttttga ggtttgctgt tggatttttt gctgaatctg
    15541 attaagatta tttgcaaaag aaaaaggggt agaaaagctt attaccccac tgaaaatagc
    15601 agtccaacag taagaattta ttcgtttcac cagcatacct t
//
LOCUS       DANLUA010000041        15591 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLUA010000041 DANLUA010000000
VERSION     DANLUA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            32..769
                     /locus_tag="PXB44_000934"
     CDS             32..769
                     /locus_tag="PXB44_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5281432.1"
                     /translation="MRGGKQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQ
                     KYRLFDLNVEDRKQHFSEIYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIR
                     NYIALNLFGAGSARRFSEQKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNI
                     FVYEKTKSVFDSIELIRFADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNN
                     QAETHILRFRKSVNDLDFAAIKPEWLK"
     gene            771..1661
                     /gene="ilvY"
                     /locus_tag="PXB44_000935"
     CDS             771..1661
                     /gene="ilvY"
                     /locus_tag="PXB44_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL5281433.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(1795..4197)
                     /gene="lon"
                     /locus_tag="PXB44_000936"
     CDS             complement(1795..4197)
                     /gene="lon"
                     /locus_tag="PXB44_000936"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL5281434.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     regulatory      4401..4519
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4695..5792
                     /gene="ribD"
                     /locus_tag="PXB44_000937"
     CDS             4695..5792
                     /gene="ribD"
                     /locus_tag="PXB44_000937"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL5281435.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     gene            6009..6656
                     /gene="ribE"
                     /locus_tag="PXB44_000938"
     CDS             6009..6656
                     /gene="ribE"
                     /locus_tag="PXB44_000938"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL5281436.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETDLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            6675..7874
                     /locus_tag="PXB44_000939"
     CDS             6675..7874
                     /locus_tag="PXB44_000939"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL5281437.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            8080..8544
                     /locus_tag="PXB44_000940"
     CDS             8080..8544
                     /locus_tag="PXB44_000940"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL5281438.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(8590..10098)
                     /gene="lnt"
                     /locus_tag="PXB44_000941"
     CDS             complement(8590..10098)
                     /gene="lnt"
                     /locus_tag="PXB44_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL5281439.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(10157..11056)
                     /gene="corC"
                     /locus_tag="PXB44_000942"
     CDS             complement(10157..11056)
                     /gene="corC"
                     /locus_tag="PXB44_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL5281440.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            11194..11802
                     /locus_tag="PXB44_000943"
     CDS             11194..11802
                     /locus_tag="PXB44_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL5281441.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            complement(11883..12266)
                     /locus_tag="PXB44_000944"
     CDS             complement(11883..12266)
                     /locus_tag="PXB44_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281442.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(12296..12865)
                     /locus_tag="PXB44_000945"
     CDS             complement(12296..12865)
                     /locus_tag="PXB44_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL5281443.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            12924..13769
                     /gene="nfo"
                     /locus_tag="PXB44_000946"
     CDS             12924..13769
                     /gene="nfo"
                     /locus_tag="PXB44_000946"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL5281444.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            13897..14469
                     /locus_tag="PXB44_000947"
     CDS             13897..14469
                     /locus_tag="PXB44_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5281445.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(14536..14994)
                     /locus_tag="PXB44_000948"
     CDS             complement(14536..14994)
                     /locus_tag="PXB44_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5281446.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(15168..15551)
                     /gene="grcA"
                     /locus_tag="PXB44_000949"
     CDS             complement(15168..15551)
                     /gene="grcA"
                     /locus_tag="PXB44_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL5281447.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
BASE COUNT         4622 a         3052 c         3297 g         4620 t
ORIGIN      
        1 tttggttaag acaaaaagta ttcctgatta tattcgtggt ggcaaacaac ttgcaaaaga
       61 gcagtatgat gttgtaatcg atccgactgt tactttacgg aatcgtgatc tcttattttt
      121 acgcactata tcagccaaac attatgtagg ttatcagaaa caaaaatatc gattatttga
      181 tctaaatgta gaggatagaa aacagcattt ttcagagatt tatcaagatg cgttagcttt
      241 aatcgggttt gaaaatatag aaactcagta tgatgtgcca ctaaatgcca caagcaacca
      301 atccataaag cattatttat atgaaaacca aattagaaat tatattgcgt taaatctttt
      361 tggtgcaggt tcggcaagac gttttagtga gcagaaaatt aatgaactgc tgagttgctt
      421 aaaacagcat agtgataaac cgatagtctt acttactttt cctgaagtaa cggcaaaatt
      481 gtgttccttt actaagaagt ataaaaatat atttgtctat gagaaaacca aaagcgtttt
      541 tgattcaata gaactgattc gttttgcgga cttattaatt tcacctgata ctgcaaccgt
      601 gcatattgcc tcaggcttga gtaaaaagat aattgctttt tacagtagcg atgaacagaa
      661 ttttatacat tggcatccga ataatcaggc tgaaacccat attctccgtt ttagaaaaag
      721 cgtgaatgat ttagattttg ctgcaataaa accggagtgg ctaaaataat atggacttcc
      781 aaagccttaa acttttccta gatatagccc aaagccgtag ttttattcgc agtgcggaga
      841 agaattatat gacggcttcc acacttaccc gccatattca gcgtatagag gaggagctcg
      901 ggcagccgtt gtttctgcgt gataatcgac aagtgcgttt aactgaagca ggggaaaggt
      961 ttttggcgtt tgctcaacaa ggttgggcag agtggcagca gttgcaaaac cagttgtcgc
     1021 ctgctcaagg tgatttagaa ggagagttga aagtgttctg ctcggtaacg gcttcttaca
     1081 gccatttacc gccgattttg ggaaagtttc gccaaaaata tccgaaagtg gatattaaac
     1141 tgagtaccgg tgatccggct ttggcattgg aacaggttca gtcattggct gcagatattt
     1201 cggttgcggg taagccggaa tttctgccga ataatattgt gtttcactat attgatgata
     1261 ttcaactttc gattattgcc cctcgtgtgg cgagccaagc cactcaattt ctgcaacagt
     1321 cgccgataga ttggaaaaat atccccttta ttttgccggt gaacggacct gtacgaaaac
     1381 ggattgaccg ttggtttaaa gagcaaaaaa tcaaagaccc gattatttat gctaccgtct
     1441 ccggacacga agcgattgtg ccgatggttg cactaggctt tggggtcgca ctgctaccgg
     1501 atattgtgat taagcatagc ccaatgaata atcaagtttc ttatgtgaat ttaccctcgc
     1561 cgatcactcc gtttgagctg ggaatttgcg ttcagcaaaa acgcttgcaa gagccgatca
     1621 tcaacgcatt ttggcaatta ttgtctgata attcagcata acaagcggtt agattttgct
     1681 aaatttttgc aaaaaataga gcaaaaccga ccgcttgtag ataaaacttt cagataaaaa
     1741 ataaggtgcg tgattgtgat ctgcgcacct tatgttgttt tagattattg cactttattg
     1801 cactgtggct ttagctcgcg attttttcac cttgatctcg gcttttgaca agatttcaat
     1861 ccccattggc gggttttcta gtgccagagc tagtacctcg tcaatggttt ctactgcgtg
     1921 aatgtccaac gctgcttttg cattttccgg aatttcctct aagtcttttt cattttcttt
     1981 cggaataatc accgtcgtaa taccaccacg atgggctgcc agtaatttct cttttaagcc
     2041 gccgattggc aatactttgc cacgcaagct gatttcaccg gtcatcgcta cttctttacg
     2101 cacagggtta cctgttaggc tggaaattaa tgcagtacac attgcaatcc ctgcactcgg
     2161 accatctttc ggtgttgcac cgtccggcac gtgaacgtga atatcccgtt tttcgtggaa
     2221 gtcttcagca atgcctaatt tttctgcacg agagcgtaca accaccattg ccgcttgaat
     2281 agactctttc attacatcgc ctaatgaacc ggtgtaagag aatttgcctt tacctgcaac
     2341 agaagcggtt tcaatggtta ataaatcacc gccaacttct gtccacgcta aaccggtcac
     2401 ttcgccaatg cggttttggc tatccatttt gccgtagtca aaacgtttta cgcctaagta
     2461 ttcttcgata tttttctcag aaactgtaat ggattttaat ttcttatcca atactaaagc
     2521 tttcacggct ttacggcaga ttttcgcaat ttcacgctca aggctacgca cgcctgcttc
     2581 acgggtgtag tagcggataa tacttaaaat tgcactgtct tcaatggtca gctcaccctc
     2641 ttttagtccg ttattttctt gctgtttagc cagtaaatga ccacgagcaa tgtgcatttt
     2701 ctcatcttcg gtatagcctg aaagacgaat cacttccata cggtcgagca atgctggcgg
     2761 aatgttcatt gagtttgagg ttgccacaaa cattacatcg gataaatcgt aatccacctc
     2821 taaataatgg tcgttaaagg ctttattttg ctcaggatct aacacttcaa gcagagcaga
     2881 agccgggtcg ccacgcatat cttgagccat tttgtcgatt tcatctaaca agaacagcgg
     2941 attacgcacg ccgactttcg ccattttcat cattaggcta cccggcattg agccgatgta
     3001 agtacggcga tgcccacgga tttccgcttc atcacgcacg ccacctaacg ccatacgcac
     3061 atatttacgc cctgtggcat tggcaataga ttgccctaat gaggttttac caacccccgg
     3121 cggaccaact aagcacaaaa tcggaccttt taatttattt agacgacttt gcactgctaa
     3181 gtattctaca atccgctctt tcactcgttc taagccgtag tggtctttat ccaaaatctc
     3241 ttgagcttgg gctaaatctt ttttgaccgc tgatttttta tgccacggca tttgtaaaat
     3301 ccagtcaata taaccacgca caacggtagc ttcggaagag ctttgcggca ttgctttgag
     3361 ctttttgaac tccgtattca gcttgtcttt tacctcggtc ggtaattttg cagcgtcaat
     3421 tttctcttta agcttatcta actcgctttg ttcaacgtca tcgccattac ctaactcttt
     3481 ttgaatcgct ttaatttgct cgttgaggta gtaatcacgt tggttttttt ccatttgctg
     3541 tttaacacga ttacgaatcc gagtttcggt ttcaagcgta tccatctcag ttgccatcgc
     3601 aattaacaag gcttcaaaac gggcaatcag atttggttgc tccaataatt cctgtttttt
     3661 cttcaccgga gcaattaagt tagaggcaat ggtatccgcc aagcggtctt ccaacgtgat
     3721 tttttgcagt ttagcaataa tttccgccgg aattttcttg ttatttttaa cgtaattttc
     3781 aaattcggtt aaagtggttt tagcaatcgc ttttaactct tcgttttcgt catcatattc
     3841 cgaatatatt ggctgaattg ccgcccagaa gccgttttca tcatcgtgaa tttgctcgat
     3901 tttggcacgg gtttgtcctt ctaccagcac tttaactgtg ccgtccggca ggtttaacat
     3961 ttgaataatg ttggcaatta cacctacgcc atacatatct tctgcattcg gctcttcttt
     4021 atttggatct tgctgagtga ctaaaaacag ttgtttattg gaatccatcg ccgagcgtag
     4081 ggcttgaatt gatttttcac gtcctacaaa taacggcata accatataag gaaaaaccac
     4141 cacatcacgc aatgggagta gtggtaattc aatcgctttc ttttttcttg gtgccataaa
     4201 tttgctctct ctatcaaaat ataatccccc attatgaggg cgaatttggg aaaaacaagg
     4261 gaaaatttga tttatcttaa aaaaactcga atagtcaaaa cagtagatgt tggcgattta
     4321 atcaattgtt aaaatcaggg taattagctt aatttcccgc ttgacatcaa atcgtaatgc
     4381 gagcaaaatc ccctgcatat tttaatcttc agggcagggt gaaattcccg atcggcggta
     4441 aagtccgcga gccgagcaat cggcaggatt tggtgaaatt ccaaaaccga cagtatagtc
     4501 tggatgaaag aagagtcaaa aaccgcaaaa aataccgcac aagcggtcat tttttgtgtc
     4561 ttttttgcaa gccttgagtc gtgagatttc aaggcttcat tgttttgcag gcggcgtttg
     4621 caaatttttt actaaaaacg accgcttgta ttgcaataat cctttcatta cgccctcaat
     4681 ttatcaaaat gtatatgaca gatgttcaat atatggctta tgccatcacc cttgccgaaa
     4741 aagcacgggg ttggacaagc cctaatccgc tagttggctg tgtgatcgtg aaaaacggca
     4801 acattatcgc cgaagggtat caccaaaaag ccggtagttg gcacgctgaa cgcaatgcga
     4861 ttttaaactg cgaggaagat ctgacggggg caaccgctta tgtaacctta gagccttgtt
     4921 gccaccacgg gcgaaccccg ccttgttccg aactgctgat tgaacgtggg atcaaaaaag
     4981 tgtttatcgg ctctcgagat ccgaacccac ttgtttctgg caaaggagcg gcacagctta
     5041 gagccgccgg cattgaggtt gtggaagact ttatgcgaac ggagtgcgat gcgctgaacc
     5101 cgattttctt ccattacatt caaaccaaac gcccttatgt gttgctgaaa tatgggatga
     5161 cggcagatgg taaaattgcg acttatacgg ggaattcgaa atggatcacc ggcgaggctg
     5221 cccgagctaa tgtacagaaa agccgccacg aatatagtgc gattatggtc ggggtggaaa
     5281 cggtgcttgc cgatgaccca atgctcaaca gccgaatgga aaatgctaaa cagccggtgc
     5341 ggattgtatg tgacagccaa ctgcgtaccc cattggatag ccaactagtc caaactgcac
     5401 gccaatatcg caccattatt gctacgctca ataatgatgc aaaaaagcag gcagaatata
     5461 ccgcttgcgg tgttgaaatt ttagtgacag gcgaacgtga caagcgggtg gatttagccg
     5521 atcttttgca aaagctgggc gaactcggga tcgacagctt attgattgaa ggtggttcgc
     5581 aactcaattt cagcgtgctg gaacaggggt tagtcaatcg agtacattgc tatattgccc
     5641 cgaaactgat tggcggcaaa gcggcaaaaa ccccgattgg tggcaagggg attggcgagt
     5701 tagcacaagc ggttaaatta cgtctaaaat ccaccgaatt gctcggagaa gatattttgt
     5761 tggattatga agtggacttg cttcgtaatt aaattgaaat ttccttactc tcgcccgttt
     5821 acgggagaga gacagcataa aatttcgttc agaaatttta tgcagagaga ggggaaaatg
     5881 agtgtaatct tccctctccc tccgaaaatc ctacggattt tctcttccct ctcccgtaaa
     5941 cgggagaggg gaatagtaag ataagcagtt ctcagactaa attggaagta aatcggagaa
     6001 aaatttaaat gttcacaggc attatcgaag aagttggcca gatagcccaa attaagaaac
     6061 aaggcgagtt tgtggtggta acggttaaag caaaaaagat tctgaccgat gtacatttag
     6121 gggatagcat tgcggtaaat ggcgtgtgtt taaccgtgac ttcatttacg caggagcaat
     6181 ttactgctga tgttatgtca gaaaccttaa aacgtacatc acttggcgaa ttaagtatca
     6241 acagctcggt taatcttgag cgggcaatgg cggcaaacgg gcgttttggt gggcatattg
     6301 tgtcaggtca tattgatggc acgggtgtgg ttgcagagat tacaccagca gataattcaa
     6361 cttggtatcg cattaaagcc gaacctaagt taatgcgtta catcattgaa aaaggctcaa
     6421 ttactattga tggcattagt ttaacggtgg tggatgttga tgcggaatct ttccgggtat
     6481 cgattattcc gcataccatt aaggaaaccg acctcggtac gaaaaaaatc ggtagtttgg
     6541 tgaatttaga aaatgacatt gtcggcaaat atatcgaaca gtttttattg aaaaaagagc
     6601 ctgaaaatcc gcaaagtaaa attactgcgg actttcttaa aaatgcaggg ttttaaccaa
     6661 ataaaggaca aataatgttt aagttttcaa cggtagaaga agcaattcaa gcaattgcac
     6721 aagggaaaat tattttagtc agcgatgatg ccgaccgtga aaatgaaggg gacttcattt
     6781 gtgcagcgga atttgcgaca ccggaaaata tcaactttat ggcgaaatat ggtaaagggt
     6841 tgatttgtac gccgatttcg acagaaattg ccaaaaaatt aaattttcac ccgatggtgc
     6901 tagagaacga agataatcat caaaccgcat ttaccgtttc tgttgaccat attcaaaccg
     6961 gcaccggtat ttccgctttt gagcgttcat taacttgcct gaaaatgctt gatgaaaatg
     7021 ctaagccgga agattttaga cgtccggggc atgttttccc attagtggca aaagacggag
     7081 gcgtattagt gcgtaacggt cataccgaag caactgtgga tttagctcgt ttggcgggct
     7141 tgaaaccggc agggctttgc tgtgaaatta tggcagaaga cggcacgatg atgcagatgc
     7201 cggaattaca ggcatttgca caagcacaca atatgccgtt tattactatt cagcagttac
     7261 aagagtatcg tagaaaacac gacagcttgg tgaatgtggt ttcggtggta aaaatgccga
     7321 ccaaatacgg tgagttcaaa gcacacagtt tcgttgaaac tatttcaggc aaagaacacg
     7381 tcgctttagt taaaggtgat attggtgatg gcgaaaatgt attatgccgt atccattccg
     7441 aatgtttaac cggcgatgcc tttggctcac aacgttgcga ttgtggtcag cagttcgcag
     7501 cggcaatgaa ccaagtggag caagagggca gaggcgtggt gctttattta cgccaagaag
     7561 gacgtggcat cggtttaatt aataaactgc gtgcttacga attgcaagat agaggaatgg
     7621 acacggttga agccaacctg gcattaggtt tcaaagatga tgagcgtgaa tattatatcg
     7681 ccgctcaaat gtttgaaaaa ttaggcgtaa aatcgattcg tttgttaacc aataatccgg
     7741 caaaaattga aggactcatt gagcaaggtt taaacgtagt ggctcgtgag ccgattatgg
     7801 ttgagccaaa cgaacacgat ttggaatacc ttaaagttaa acagcacaaa atgcgacata
     7861 tgtttaattt ctaatccaat actctctccc cttgtgggag agagacagaa aaatacttcg
     7921 ttcagaagta tttttcagag agagggtata ttttttagaa aaagtttgca atttcccctc
     7981 tccctgattt ttcttctgaa cgaagaaaaa tctatccctc tcccacaagg ggagagggtt
     8041 attgagtatc aaatttattt taaagaagga aaacaaacaa tggcaacatt tcaaggaaac
     8101 tacatcgcaa ctggtttaaa attcggtatt gtggcaacta actggcacaa gatttttatc
     8161 gatcaactgt taaacggtgc aaccgataaa ttattgcgtc acggcgtaga agaaaaaaat
     8221 atcgacaccg tatgggtgcc gggtgcatta gaaatctcaa ttgcagcgaa aaaaatggca
     8281 caaagcggtc gttatgatgc gattatctgc ttaggtgcgg tcgtgcgtgg agcaaccagc
     8341 cactatgatg tggtagtgaa cgagtcagca aaaggcatca gctcttctgc tttagaaacc
     8401 ggcgtgccga ttattaacgg tattttaacg gtggaaaatt tagagcaagc gattgagcgt
     8461 tcaggcacta aagccggtaa taaaggcgaa gagtgtgcaa tggtagcgat tgaaatggcg
     8521 aacctattaa aagccttagc gtaatcagcg ataaaatccc cttagaccca agagcctaag
     8581 gggatttttt tattttctgc gtagtaacag gctggcaatg acacaaaata ggctgaaact
     8641 gtaaattaac cagtgaccta attgtttaaa cggcgtatcg cctttggttg gcacaatggt
     8701 ggcagttagt gcatcagcag taaattgcgg tagttgttca attacttttc cgttggcatc
     8761 aacaatggcg gtaataccgg tattcgcagc tcgaagcaat ggtttgccaa gttctaacgc
     8821 tcgcattctt gccatttgga aatgttgcca aggcccgatg gaagaaccaa accacgcatc
     8881 attggtgata gttaataaat aatctgaatt ttggcttttt tggttttgtt gtaactgatc
     8941 accaaaaata atttcatagc aaatcgccat attaaatcgg ttgtttttcg ccaagaatga
     9001 tggttgaacg aaactgcctt gtgaaagatt aattggtaga ataaacactt cccgcatcca
     9061 atccagcacc gagccaaacg gcacatattc gccaaacggc acaaggtgat gtttgttgta
     9121 gcgagggttg ctgtgcaggg catattccct ctctttttcg cctaatagca cgctactgtt
     9181 aaacagttcc tcttgctcat tttgatagag tgtgccgata ataatttcac ttccgtgttg
     9241 gcgagctaag tgatcgagcg agccaagcag cgggctgatt tgattttcaa gtgccggaat
     9301 ggcggattca ggcaaaataa tcagttcact gttacctaat aggggcagaa tgagctgttc
     9361 gtaggttcgt acgctgtggt tgaaatgttg cggatcccac ttcatttttt gctcaatatt
     9421 cccttgtact aaactcacta caagcggttg tttttctgta tcaatttgca caaattggag
     9481 aaagcgagtg gcaaaactta agccaaaaat gaccgctagt accatcaatg atggcacagt
     9541 tttgttctct tgctgaataa ttttcactag atagctactt gcaactatta ccaagaaagt
     9601 caaaccttca acgcccaaaa tcggcgcaat accagcaaag gggctgtcta ttaagctata
     9661 gccgaattgt agccacggaa aaccggtaaa taccacgcct cgtaaatatt cggtaaaggt
     9721 aaaaatcgca gccaatgcaa acggattatt cagtttgagc ctgtgtgaaa ctacactaaa
     9781 cagcagatta taaatagcga gatagcacgc caacaggaat actgctatgt agcttactat
     9841 ggtaggaaca ccaccaaatt gggtcatact cacagaaacc cagtttacac cggtgcagaa
     9901 gtagccgata gaccaagcaa aggttgccca taatgcggtt tttctgtttg ccagagtggc
     9961 tgaccaaacc aagccggctg cggaagcaaa ggcaagaatc caaatatcaa aaggcgaata
    10021 ggcgaaagtg ccgattgcac ccagtatgat tgcaaggaga caagcggtta aaaaaggcgg
    10081 attttttgca aaattcataa taactcacta aataaatgag gcacgccgaa acgtgccttg
    10141 attgaaactc actatattat tcttcttcgt taggagaggt tttttccatt ttctctaact
    10201 gttcatcgga aagtgtgact cgtagctgaa tcaattggcg gctattagct gaggtgactt
    10261 taaaatcaat tccgtttaac tcaatttgct catctcgttt cgggaaataa ccgaaagcct
    10321 gcatcactaa accaccaatg gtatccacct cttcgtcatt aaagtcggta gcaaaaaatt
    10381 cattgaattt tttgatgtcg gtgagcggta ataccgcata agtatgtctt gaaagctggc
    10441 gaattggttt gacctcttct tcatcaaact catcttcaat atcacccaca atttgttcaa
    10501 gaatgtcttc aatggtgact aagcctgaaa ccgcaccaaa ttcatcaacc actactgcca
    10561 tatggaagcg ttctgaacgg aactctttta gcattcggtc taggcgtttg ctttccggta
    10621 caataaccgc agggcgtaat acctgttgca gattaaaggg ttcagagtca gaacgtaaat
    10681 atttcaataa atctttagag tgaagaatac cttcgatagt atctttctca ccccgaatca
    10741 ccggaaaacg agagtgtgcg gattcaataa ttaaatcaat acagacatct aaaggttcat
    10801 tggcatcaat aaacacaatt tgcgcacggg gaatcataat atcccgcact cgtaattccg
    10861 agatttccat tactccttcg atcatctctt tggtgtcgct gtcgatcaat tcattttcaa
    10921 cagagtctcg aatcacttcg actagatctt cacggttttt aggctcgttt tgaaaaagcc
    10981 cgctaaatag agagtgtaaa atcgattttt tttctgattt ttcggtggtg tttacacttt
    11041 gctgttcatc actcatagtt tgttccactt aaataaagta cgcttataat aagcaaattc
    11101 agtcacaaag caaataaaac attgctattt tcactcaaat tagcatagga taagcaaaaa
    11161 tctttttatt tcttcatatt ttaggagttt gaaatgaaat taaccaaaat agcattaagt
    11221 ttaacagcct tagttgtcag ttcgatgagt gtggccggga cgttaagtac atcatcaatg
    11281 gtggacattt tagcctttga cgggcaacgt gttaaatccg gcacgacttc ggttcaaatg
    11341 agtgagaata aaacgcatca agtggtagtg gaagtcggtg ggacagttga aggggatttt
    11401 ttcacctctg atcaaatcat tttgactttc caaggcagtg cggaaaatgc aaaattagaa
    11461 acgccaagat taaattcaaa gttagatctt cgtaaattcc aagagaatca gactgtggca
    11521 attaaaaccg attcaggtaa ggttatttca cataaacaag atttcttaaa aggggaaggt
    11581 ttccttgcga aaacaagagt tgaagataat ttaacgaaat ataacttagc gaaaaaaaca
    11641 gcttcagttg aacagtttgc gacggcacct cttgaagcaa aaggacaaat tgtggtggat
    11701 acaaataaag tttcacaaga agaattacag cttttattta gaaaggcaga taaagacacc
    11761 caaaaacgtt tcttagaatg ggcgaagaaa aatataaaat aaacctatat caattaataa
    11821 gcggtcattt ctcgccaatt ttttgcaagg tgttaaaaaa atgaccgctc atacaatgag
    11881 atttaccagc aataatagca gtaacgccag cctctataca tatagtctct atcgtaattc
    11941 caacgaattt cttcaagttc tacttgtgcc ttgtaatttt gccaagcaag cttataacag
    12001 gcttggaaca ttggggcatt gactttttcg gcatagcgtt tcttaaatgc tttctcttct
    12061 tgttcggttt ggctaagcgg cggattaaat tgttggtaca ttaactccgc cgtttctaca
    12121 tcacattgtt tggcgagctg aacttgcaaa tcttgttctg cccgaatttg cttcgcttta
    12181 cgttcagctt gttgctctgg agttaaagta cagctactta acataagtgc gagtgaaaaa
    12241 atgcagccta atttgagtgt tttcattgct gaatcctcta gttgaaattg gtgatttatt
    12301 ctaccgcttc taactcactt tcttcaagtt catcagccat agcactaagg gcatcacggc
    12361 taagttgtgc gagggcttta taaaaaccat tgtcgaaagc ttctattttt cctaagaatt
    12421 tgcctgcaca aggtagtaaa aattgttcga ataatatttt ttgagctaag gcagaatcga
    12481 gattatcttc gatccaagat gccgtgagaa atagtaaccc aatgtgatcg gcattttcta
    12541 attcaggcat tgcccgctct tgtctaaaag ctacaaaatt attaactgat aatccatagg
    12601 ctgaaattgt tggagaaata gccccatttt cagcaaaaag cgcttgatag cttgctgcta
    12661 attgcgtagg gttagcattc ttttgtaaga gagtcagcgc attttcactt tggctatccg
    12721 tatttaatgc ccagacttgg cgtaatcctc cttgggcaag ccagttaaat gtgccagcta
    12781 aaatagggtc ggtagggtga cgataaaata agttgccaaa caaacggcaa agcagggaaa
    12841 aatcgttaat ggtagtttcg ttcataattt gaaatagata gaaaaaagaa ttgttagtat
    12901 agcagaacac aagagaggac tgaatgaaat atataggcgc acacgttagt gcttcaggtg
    12961 gggtggaaaa tgcagttttg cgttccgtag aaattggtgc taatgcattt gcgttattta
    13021 ctaagaatca acgtcaatgg caggcaccgg cattaaaagc cgaaactatc gaaaaattta
    13081 agcgtttttg taaagtgcat catttttcag cagagcaaat cttaccacac gatagctatt
    13141 tgattaattt aggaaatccg gaagcggagg cattggagaa atctcgtgta gcatttattg
    13201 atgagatgtc tcgagcaaat caattaggtt taaaattgtt gaattttcac ccgggggcgc
    13261 atttaaagca aatttccgag caagattgtt tggctagaat tgcagaatcg atcaatattg
    13321 cagttgaaaa agtaccgaat gttattgccg tgattgaaaa tacggcagga cagggttcta
    13381 atttaggttg gcatttcgat catttggcaa ctattattga gcaagttgag gataaaagcc
    13441 gagtgggggt gtgtttagat acttgtcact tattttctgc cggctatgat attagttctt
    13501 atgaaaagag cgaggcagta tttaatgatt ttaaccaaac ggttggtttt cattatttaa
    13561 ggggaatgca cttaaatggt tcaaaaacac cattaggcag cagagtggat cgtcatcata
    13621 ccttaagaga aggcacaata ggaaccgaag tgtttgcatt tattatgcaa aatatgcatt
    13681 ttaaccatat tcctatgatt ttagagacaa tttcgcctga aatttgggca gatgaaataa
    13741 aatttctacg cacattggaa ctaaaatagg ttatactggt ctgagtagtg ggtctagcag
    13801 agtctagacc ttattttgtt atctttttta atcttttaga agatttagtt aaggaattta
    13861 ccgctgtaat tgttctggcg agacgagaca taaattatga aaaaaaatct ttttgcaaca
    13921 accttcaagc ccgtatttat tgcttctatt ttgtctgttt ttggagggta ttcttccatt
    13981 gctgtagcaa gtttaagttc taaaccggcc acaccaaatc aaatcattgc aagtaaagca
    14041 gaaagattag ctaagtcttc ttcagtattt accaatcgag caaatcttcc aacttcttcc
    14101 gctcatacag cgaattatag tggtcgtatt aatagtgttt atcataattg ggtagggaca
    14161 cgctatcgtt taggcggcac aacaaaagca ggaattgatt gttccgcctt tgtgcgtgaa
    14221 gtaatgggca aagcatttag taaagatatg cctcgttcaa ctgcagaaca acgccatgtc
    14281 gggcattcca tttcaaaatc tgatttacgc ccaggtgatt tagtcttttt ccgtaagaat
    14341 aaccatgtag gggtttatat tggtaatggt aaatttgtac acgctagttc aagtagaggg
    14401 gtaaccacca gctcattgtc agaaagctac tggtcaagaa actacacaca atctcgccgt
    14461 gtaatttaat aaattacaat tccaagatga aaagaacact cacttttgcg agtgttcttt
    14521 tttaattaaa aaagtttatg ctacgcgttg cttagcttgt gtaaatgctc cacgagtacg
    14581 catttgatga accacattag taattacacc tgctattttt acttcagccc aatcagtaat
    14641 tctaagattt gattcgctca catctaatga aaataaaatt ttttcagcaa taccttcccc
    14701 attctgaatt tcattaaaga attgatagaa tttatattcc ccatcttttt caagcaccaa
    14761 tagatcatta actaaatatt ggttggtctg ttctacaatc aataaatcgt ctagttcaat
    14821 tccccacgcg attaaatttg ggtttttcac gtggataaaa aaagtgtctc ttgggcgttt
    14881 gatacaaagt gagttaagat cgagctttac agagcggctg ctattgaaat cccgataaag
    14941 cggaatcggc tgataagctg ccgtttgatc tgaaatttgt ctaattgaag ccatagtaaa
    15001 atcctctatc tgttttttta tacagggtat tttactggct aaaataccag tgtcaaccat
    15061 tattttggaa ttttttgtaa atttaaccag tgttttgacc gcttgttatc ctttaaaaag
    15121 caataaaaaa gcacccatag ggtgcttctt atgcttaaaa gctgttatta taggctttca
    15181 gtgaatgtac gagtaataac atcgcgttgt tgctcagggg ttaatgagtt gaaacgaact
    15241 gcataacctg aaacacggat cgttaattga ggatatttct cagggttttg aatcgcatct
    15301 tctaatgttt cacggcgtaa aacgtttacg tttaagtgct gaccaccttc aactttaatc
    15361 gttggtgcaa cattaacggg tacttcacgg tatgcgatat cacctaattc gctgatagca
    15421 acaacttgat cttcttcaaa tcctgctttt gcagctaaac aacgagcttc atttttagct
    15481 tcgtctaata accagaaaga gtttaataag ttgctgtttg atgattctgt gatttgtaca
    15541 cctttaatca ttttgatatc tcctctaaga ttgaaattat aaaaaaattt t
//
LOCUS       DANLUA010000042        14876 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLUA010000042 DANLUA010000000
VERSION     DANLUA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..281
                     /locus_tag="PXB44_000950"
     CDS             24..281
                     /locus_tag="PXB44_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5281448.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(328..948)
                     /locus_tag="PXB44_000951"
     CDS             complement(328..948)
                     /locus_tag="PXB44_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL5281449.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(950..1603)
                     /locus_tag="PXB44_000952"
     CDS             complement(950..1603)
                     /locus_tag="PXB44_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL5281450.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1652..2311
                     /locus_tag="PXB44_000953"
     CDS             1652..2311
                     /locus_tag="PXB44_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL5281451.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2348..3394)
                     /locus_tag="PXB44_000954"
     CDS             complement(2348..3394)
                     /locus_tag="PXB44_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL5281452.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3394..4347)
                     /gene="dusC"
                     /locus_tag="PXB44_000955"
     CDS             complement(3394..4347)
                     /gene="dusC"
                     /locus_tag="PXB44_000955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL5281453.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4425..5051)
                     /locus_tag="PXB44_000956"
     CDS             complement(4425..5051)
                     /locus_tag="PXB44_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL5281454.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(5051..5908)
                     /gene="djlA"
                     /locus_tag="PXB44_000957"
     CDS             complement(5051..5908)
                     /gene="djlA"
                     /locus_tag="PXB44_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL5281455.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5956..6666)
                     /locus_tag="PXB44_000958"
     CDS             complement(5956..6666)
                     /locus_tag="PXB44_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5281456.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6902..7387)
                     /locus_tag="PXB44_000959"
     CDS             complement(6902..7387)
                     /locus_tag="PXB44_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5281457.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(7414..8700)
                     /gene="tolB"
                     /locus_tag="PXB44_000960"
     CDS             complement(7414..8700)
                     /gene="tolB"
                     /locus_tag="PXB44_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL5281458.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8716..9939)
                     /gene="tolA"
                     /locus_tag="PXB44_000961"
     CDS             complement(8716..9939)
                     /gene="tolA"
                     /locus_tag="PXB44_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL5281459.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9953..10390)
                     /gene="tolR"
                     /locus_tag="PXB44_000962"
     CDS             complement(9953..10390)
                     /gene="tolR"
                     /locus_tag="PXB44_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL5281460.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10532..11212)
                     /gene="tolQ"
                     /locus_tag="PXB44_000963"
     CDS             complement(10532..11212)
                     /gene="tolQ"
                     /locus_tag="PXB44_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL5281461.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(11234..11635)
                     /gene="ybgC"
                     /locus_tag="PXB44_000964"
     CDS             complement(11234..11635)
                     /gene="ybgC"
                     /locus_tag="PXB44_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL5281462.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
     gene            complement(11798..12085)
                     /gene="ybgE"
                     /locus_tag="PXB44_000965"
     CDS             complement(11798..12085)
                     /gene="ybgE"
                     /locus_tag="PXB44_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL5281463.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(12085..12183)
                     /locus_tag="PXB44_000966"
     CDS             complement(12085..12183)
                     /locus_tag="PXB44_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL5281464.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(12198..13331)
                     /gene="cydB"
                     /locus_tag="PXB44_000967"
     CDS             complement(12198..13331)
                     /gene="cydB"
                     /locus_tag="PXB44_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL5281465.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(13344..>14876)
                     /locus_tag="PXB44_000968"
     CDS             complement(13344..>14876)
                     /locus_tag="PXB44_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL5281466.1"
                     /translation="LSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTGKEVYKD
                     MTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLMAFFLES
                     TFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFNFETMRM
                     ELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKRSFSVGA
                     SFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPNTAEMKN
                     EFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSGNYTEAD
                     KEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRVMMLSGF
                     LMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQPWAIYEM
                     LPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGRYYFEQS
                     TK"
BASE COUNT         4514 a         3279 c         2655 g         4428 t
ORIGIN      
        1 tatttgtacc taatataggt gcaatgaaaa atcttagatt atcaatacat tccagcgagc
       61 atatttggtt aagagaacta ttcttaaaac gccgtttgga actagggcta acacaacgca
      121 cattaggaga aaaaatgggc gttctttact catttatcgg aaaagtagaa acaggagatc
      181 gcagattaga tatttttgaa tttatcgctt attgcaaagg gttagattta gatcctttat
      241 ctgtattgca agaaattgaa caacaatttc ctcataaata aaggcattga gttataactc
      301 aatgccttta ttttctatgt caaatctcta catgctcggc ttagtctctg ccagaatggc
      361 gtatttatta ttcatttcct ctgcccaagc ggttaaattt gggtaatttt ttgcaagatc
      421 gtagcctatc ttatctaagc ggaatgccca ccagtcaatt aatgctactg cggttaatgt
      481 gcctaaattc agctctgtgc caaactcttg caatgcctct tctaattgtc tgaacgaacg
      541 gatattgcgt tccattaatt gctcgtggcg gctgacccac cattcattct cggggcgagc
      601 catacgttct tgtaataaag ttggcatcgt attttccata atgccgtccg ccaaattatg
      661 taatgccaac actgcccagc gaggtttacc ctctttaggt aacagtggag tgttattgcc
      721 tttttggtca agatattcgc taattaacaa actaccgaaa agccaacgtc cgcagttgcg
      781 ttgtagggca ggaatacgtc ctaacgggtt atcttgatta tgtggcgaat ttggatccat
      841 tgcttttgtc acccgcaaca tttcgacttt atcgtctaat tggtggtgtt taatcaccgc
      901 aagggctttg cgggcgaaag ggctggtggt tgagtaccaa agtttcatac tattctccgt
      961 gtttgatgtg attggatttg caaatttttt gccaaaaatg accgcttgtc ggcaaggctt
     1021 gcactgagat tttctcattt tcgtattcaa aatcaagctg ataagcgtgc aaataaacac
     1081 ggtcggcttg gctacctgaa tagagtttat cacctaaaat cgggctgccc aaacttttca
     1141 ttgccactct taattggtga gttttgcctg ttttgggttg caaaataaaa tgcctgagcg
     1201 agtcttcaac agaatgagaa ataaattgcg ttactgccgg gttttcctta ctgtggcata
     1261 atttccacgc tccatttctg cttttttgca tatcgccaac aattctaccc tgctttttct
     1321 tcggcttttt atcgcttaat gcccaatagg ttttgtagat tttatgctgt tcaaataaat
     1381 ggtaaaacct tgctgccgcc tctttattca acgctaaaat cagtaaacct gaggtgattt
     1441 tatccagacg gtgaaccagc caaacctgct ttacattcag ttggcgagcc actaattccg
     1501 ttaagccgat ttcagcctca tctttatgca cactcaagcc ttccggctta ttgataataa
     1561 taaaatcacg atgttgataa caaataggca atgttggggt cattttgcta taatccttcc
     1621 aatttttgac tattttagca aggaaattac aatggctgtt accatttatt tagtacgaca
     1681 cggcagaacc gtgtggaatt tggaaggcag attacaaggc tcgggcgatt ccgctttagt
     1741 agaagaaggg attatcggag cgaagaaaac cggcattgcg ttaaaacata ttccgtttac
     1801 cgccgcctat tccagtatgc agaaacgggc tcaagatacc gcaaactaca ttttagcaga
     1861 aaatgaacgc agtaatattc cgcattttca tcataaaggc ttgaatgaat ttgattttgg
     1921 cagctgggaa ggaatgaaat ctgtcgattt gcaagaaaat gatgaatatt gggtgatgaa
     1981 acgtacaccg gcagaatatc tggcaaaagc aaacggtggc gagcgatttg agcaacttta
     2041 tcaacgggtg actcaggcgt tcaatcaaat cgccgagcta caccaaaatg acggcaaagt
     2101 gctgattgtt tcacatggta tgaccttaac cttactcacg gctgtgttaa aaggcattgc
     2161 gtggcaagat ttccgcaatg aagaaaaaca tagctttgtg ttcaatactg ccattacgaa
     2221 agtagaagta gagaatggca aagtaacggt gctggaattt aataataccg accatttgga
     2281 taatgtgcca acaagccaat acaaacgata atattaaggg cggaacattg tccgcccata
     2341 gcattttcta atcagagaac actttttctt tcgtttctgt tttagcaaat accgtatttt
     2401 tgaccgaagt aagcaactcc tgacgaatag ctgaaattct tggcttctca ttcgcttgaa
     2461 aagcaacagg acgacaaaat tccattgctt taatgccaag gcgagcagtg agtaaaccca
     2521 caccaatacc ttgtgcggca cggagcgata atttcgccgt aaggttttgc gagaagaaat
     2581 ccattccaac atcagtagcg atttcggttg caccggcgaa caccatattt ttcagcacca
     2641 ttttgaacag ttttaagcgg ctgatataac ccaattccat tccataggct ttggtaattt
     2701 tattcactaa agcgatattt cttgccgcca ttaacaacac atcaactatt gccagcgggc
     2761 taaccgccac aatcaccgca ttttctgccg cattgcggga aatcatctgc ttaacctgtt
     2821 tatctatcgg ctttagcaca ttttcgctaa acagatatag tacctctttg gcattataag
     2881 cctcgttcag ttcgcttttc caaagttgct cggcttgagc aatgctcggc aagtgattaa
     2941 gctgatttgc aatattttcg caaaatttga ccgcttgttc gccatcattg gaaggcagct
     3001 gttccagcaa taattggtta ctcatttgtt gatttttctg atgatttcgc aaatacacca
     3061 actttcgcca ttcggaaata atcgccccaa cgcccaacaa gctgacaata aaaaagacta
     3121 tcgagaacgc aaaatagatc cactctcttt gctggaacgt atcaatcaac cactgaatac
     3181 tttgagcaat taccgccacg ccaaacaagg caagtgtggc aagtagtact cgaatccaaa
     3241 agcgggaagg cttaaggctt tcttcaataa tcagctccgc ctcaactgct ttttcctctt
     3301 cttcaataat cgcattttcg agatcaaact cttgtttagg ttcaaaatgc tcggttgttt
     3361 ttagctcttc ttcattaaaa atttgcttac tcattattcc tccactgcct tttcaatatg
     3421 tgcttttaga ccatcatagc cgtgttcggt tttcaagata tcaaataatt ctaccgcttg
     3481 aggatattct ttatccaaat aacgtagcca ttgtttgatg cgagcaacgt ggtaaaaacc
     3541 ggtatcaaac tgattttcca tctgaacgta cttaaacaat agctgtagca cttcattcca
     3601 cgccattttc ggaacattat atttcaccac tttgcttaaa ttcggtgtgt ttaacgcccc
     3661 acgccccatc attaatgcct tgcaagcggt tattttttgg caatttttcg ccgattcaaa
     3721 atcccaaatt tcgccgttgg cgataacagg aatagacagc cgttgttgaa tttcgccaat
     3781 cgcctgccaa ttaattcttt ctgctcgata accatcaact ttggttcgcc cgtgaacggt
     3841 aatttcattc gctccgccct gttctaccgc atcggcaatt tcaaaacatt ggctaacgga
     3901 atcccaacct aatcgcactt ttacggaaac gattttatcg cttggcacag cttcacgcat
     3961 cgctttggtt gcacgataaa tcagttccgg atcttttaat aacgaagccc caccattgct
     4021 gccatttacc gttttagagg ggcagccaca gttgagatct accccgtgtg aaccaagctc
     4081 aatggcaaga gctgcatttt ctgccagcca ctgtgggtgt tgccctaaaa gctgtacccg
     4141 cactggtgtg ccggaatcgg ttaatccgcc ttgatatagc tcaggggcta ggcgataaaa
     4201 ggttttcttc ggtaaacgtt gatcgaccac tcgcacaaat tcggaaatac agagatcgta
     4261 gtcattcact gaagttaaaa ggttgcggac aaaagggtct aaaacccctt gcatcggggc
     4321 aagaataacg ttaggaattg gattcattgg taaaataaaa aatacaagcg gttatttttt
     4381 ctcaacattt tgcaaaaaat tggcaagaaa caaccgcttg aaggttaaaa atgtctatcg
     4441 tgaatgcgca aatcggggcg agcgatcagg taaatcatca gtgcggttaa tgccccaaga
     4501 gtatccgcaa gcatatcttt ttgagcgtcc caaatatccc cttgcgagcc tagaaattcc
     4561 acgccggcat tgccaccttc aatcacggca tattgccatt caataatttc gtaagctgcc
     4621 gccaccgaca taatgaaaaa cagcgagaag aaaaatgcta cgcccattcc gcacttgcgt
     4681 ttacgcagta accattctgc catcgggaag ctgtaaaaac cgataatgta atgtgctact
     4741 cgatcaaaat ggttacgatc ttcacctaaa atcggggtta gaaattgatt tgcccactca
     4801 aacggcacat tggcaaaggt atattttgcc ccgatcaagt gcataatcag ccaaagcgac
     4861 atcaaaaaat aagctaaccc actaaactga aatttagggt aagtgataat taaaagggca
     4921 aaaacgataa aaatcggtat cacttccgca taccaaactg cgcggtctgt cggatttaac
     4981 cctgaccaaa ccgcaatcac ggtaataaca atcgctaaaa atagagggat gatatgcgag
     5041 cgtgaatcca ttatttccac tctttcactt tacaaattaa atcgtacgcc gcttgaattt
     5101 gttgagcttt ctcttttgcc aactccatca tctcatcagg caaaccttta gcggctaatt
     5161 tatccgggtg atgttcgttc attaacttac gataagcccg tttcactgta ttttgatcgt
     5221 cttgtgcggt tacgcctaat acggtatagg ctgcctcaat gcttggttgt gcaggctgct
     5281 gataactgcc ctgatagcca ccttgagatt gctgatgata actcccattt tgctgataat
     5341 agccaccatt gcggaattgt tgagctgcca taatcattgc gatcatctgc tcaaattgga
     5401 agcgagacat tcccattgtt tcggcaatcg taaacagaat ttgttgctcg ttcacatcaa
     5461 gttgcccatc ttgcaaggct gcctgcattt gcacttcgac aaagaaacgt aataagtcag
     5521 ttcttgcccc acaggcttct ctgaactctt gaatgacttg gcgtaacggg aagtcaggct
     5581 ctttacctaa ggtaaaggca ttttgcgcta attgctgggc tgcactatct aattttaagc
     5641 gagccattaa cgctcgggct aattcaatat ccgcttcagt aacacgccct tttgctttcg
     5701 caatatgccc caacaccgca aaagtggttt gggtgaaaag cgattggcga gttaaatttt
     5761 taccgaaaag acttgaacga actgaaccta attcatagag ttttttatcg gctaaatgcc
     5821 cgataaaagc gccaagtatc ccaccaaata aaccgtgaaa aagctgatag cccaagaaaa
     5881 aaccgactat ttttccgata aattgcattt ttttatcctt ttatagaaat gaataaacgt
     5941 tattctaaca aaattttact ttgattgctt tttagttaat acaaacataa gccagcttga
     6001 gacaaaaata attgcataac ctagccaact caacatatcc gggtattgtt caaagaacaa
     6061 caaccctaaa attgcactaa atagcacaga ggcataacta taaattgaga tgtctttcgc
     6121 cgccgcatat ttataggcgg tagttacaca aaactgcccg accgctgaag ttacccctgc
     6181 taacactaag tagattgtct gtaaattggt tagcactaca taatcaatgg cgacaaaagg
     6241 caataatatc aaggtcgaaa aggcagaaaa aaagaaaaca atggtattag gtgattctac
     6301 ttttttggtt tttgccaata accttaagca taaataggca atggctgcaa acacagagcc
     6361 aatcacgcca accagataag gcataaagcg aatatcaact gccggtttga taataaaaag
     6421 tgccccgata aaggcaataa ggcataaaat ccattgcagg agagatgctc tctcttttaa
     6481 aaaaatcgca gaaagaataa ttaaaatgaa cgacgtgagt ttgccaatca tatcgacatc
     6541 acttagcaca aggtgatcga tgatatatac accacataaa atccccaata acccggtgat
     6601 ggaacggctt aataataaca aacgattttc tgcataacca aagaataatt ggcgattttt
     6661 ccacacaata taaccgctta tcaatgctgt gcgttgcgaa aaatcgcttt ttgtgcaatc
     6721 ggtacatccc ccgaaagacg aagaaaagca gccataatcg caaagcttag tgccgacaaa
     6781 agcacacaaa aaatggcttt aaataacggc gagagcgaat tccaaaaatt catagcataa
     6841 aggcttttaa taaaaataaa gccctgatta ttcagggctt caatttagaa tcaactaaga
     6901 attagtattc taatactgca cgacggtttt tcgcgtagtc agcttctgag tgacctaata
     6961 ctgctggttt ctcttcaccg taagaaacag ttgaagtgtt gttagcacct ttagctgcta
     7021 agtaaccttt aactgcatct gcgcgacgtt gacctaatgc gatgttgtac tctggagtac
     7081 cacgctcatc agcgtgacca gcaatagtta cgttaccttg agtttttgct aataattgag
     7141 cgtgagcgtc taataattga cggtattcac cttcaactgc atagctgtca aaaccgaagt
     7201 atacagtgtt gtattgtgct tttaattgtt cagctgttaa accaccgaat tcgcctgctg
     7261 catttgctgc tgcatttgct gctgcgtttg ctgctgcgtt tgctgctgca tcatctgatg
     7321 agctgctaca tgctgctaat gcgaaagctg gtactgctac cattaatact ttagcaaatt
     7381 ttttcatttg ttgctcctaa tagagaaata ttattattta gttaagtatg gtgaccatgc
     7441 agggaattta taatgtccac ctgcgccagg caaattagct ttaaaacgcc catctgcaga
     7501 caccaattgt agcactttac tcgtaccttt ggtagagcta taaataacca taatgccatt
     7561 cggtgaaata cttggacttt cgtctaagaa agttgaactc agtacttctg tgccacctga
     7621 agcgagatcg cgtttcacaa ctctatcacc ggcaatcatg attaaattct taccatcaga
     7681 tgatactttc gcattatagc taccgctacc gcccattggg ctagcaccac cacctgatga
     7741 actcattcta taaacttgag gagaacctgc acggtcagac gtaaagtaga ttgtgctacc
     7801 atctggagac cagcttggtt cagtattatt tccagcatta gcggtcaatt tacttggtgt
     7861 accaccgcca ctacctacaa cataaatatt taactcacca tcttgatttg aggcaaacgc
     7921 aatgcgagaa ccgtctggag agaatgatgg cgcaccatta tgtcctttca atgctgccac
     7981 tacacggcga gcacctgaac gaatatcgtg aacaacaacc tgcgcttttt tattttcaaa
     8041 tgtcacataa gctaatctgc taccatctgg tgaccactct ggcgacatta aaggctcttt
     8101 actggttgta atagtaaagg cattaaaacc atcataatca gatactcgta attcatacga
     8161 tgatacgcca cgttgaacca catacgcaat tttagttcta aatgcaccgc gaatttgggt
     8221 taatttttca aatacctcat cacttactgt atgcgcacct tgacgaattt gggctgccgg
     8281 aacaataaat gaaccttggg caatgacacc acccgctgta cccgatgtac caagagtatc
     8341 aattaattga taagcgacat tataaccgcc acctgtgcta ctcacttggc caactacaat
     8401 attatcaata ccgatagcac tccattgatc tgccaccact tccgctgctg atcccggttg
     8461 agcaggcatt tgagagcgtt caagcggtgt aaatttacca ctatttcgca aatcatcaga
     8521 aataatttgt gcaacatctg ccggcacacc gctggcttta aatggcacaa ccgcaatcgg
     8581 ctgagccata cttacacctt catcaataga aattcgcaca tcagaatcag caaccacctg
     8641 agttgtcaat aatgctgaaa agatgcctat tacacccgat atacgagaga ataatttcat
     8701 ttgtagtcct ctaaattatt taacttttaa actaaaatca agtgttggtg atttaaacat
     8761 attatatacc gcatctgatg gtgcaggtgg tacttttcta gttgccacta ttgcacttac
     8821 cgctgcatca catactgctt tatcgccact gataacttga tagttagtaa ttgtgccatc
     8881 tcgacttaag aatatcttaa catcacattg ttttccggca aaacttggat caacccgata
     8941 tcgagattga attaatttct taattcgttg accatatgca ttaccatcta cattagtacc
     9001 ttgccctaag ccacttgtac caccagaacc ttgtgatcct gctgagtttt tgttaccacc
     9061 tttacttgaa ccaccaccga tgtctccact attgaggaaa tcatcaagtg ctttattgtt
     9121 tttcgcttgg gcggcttttg cctcagcagc tgccttcgct ttagcttctt tctcggcttt
     9181 ctctttggct tctttttctg cttttgcttt ggcttctttc tcagcctttt cttttgcttc
     9241 tttttctgcc ttggctttag cttctttttc tgctttttcc ttcgcttctt tagcttcttt
     9301 tgcctgctgt tccttcagtt ctttttgctc ttttaacttt ttctcagctt ccgctttctt
     9361 ctgctgttca agcttctttt caagtgcttc tttagctttt ttctcttctt cagcttgttt
     9421 agcagcagcc tctaaacgct tagcttctgc atctgctttc agttttgctg cttcagctgc
     9481 ttttttacga gttgcttctt ctgcttgctg ctttttaagc tcttcttgct tagcttgttc
     9541 ttgcttttgc tgttcttgta gtttttgctg acgctcaatt tcttgctgac gctgtttctc
     9601 ttgaatcgcc gctaactcag cttgtttctg tttttggatc tcttccggag tcggtttatc
     9661 ttccacatct tcttcaacag cttcctcttt aggctctacc tttggcttcg atgcttttga
     9721 gccttttttc tctgccaata actgcccata ttcagcagcc acttgcccag tatctaccat
     9781 cacagcttca aagctatcgc tatcgccacc actgccacca gccgcagcga gttccgtctt
     9841 agtgaaaaga gacccgagca ataacaaccc gattaacaat atatgcaata agatcgaaac
     9901 aataaccgct agctctagtc tatcttgttg agatttcaca cgttatcctt ggctatttac
     9961 cttctgtcat taaacctacc gatttcactc cggcgtcttt aagtagtgta atacctttca
    10021 taatttcttc ataaggtact actttatcac cggcaactaa aaataaggta ttgttatctt
    10081 tttgaaattg ttcccctgca taggcaacaa cttctgattc attaatattc tcttgcccat
    10141 tactttgcac atagttaccg tcaattttca atttatattc accaacacct gccacctcca
    10201 aaatcactgg agttttatct tcattagaaa ctgattggct atgcgtatct tccggtaaat
    10261 taacttctac actttgactg ataattgggg ccgttgccat aaaaatcagc aataatacca
    10321 acaatacatc aagaaatgga acaatgttga tttctgattt aatgtcatta cgttttaaac
    10381 gacgagacat atattcctct ttacaggtgc ttagcacctt aattgatctg aaataaccgg
    10441 cacagtgtcc cggtctcgaa taatgcggtt acaagcggtc ttttttacga aaaactttac
    10501 aaattttcat taaaaatcaa ccgcttgtag attatctttt tgaaaaagcc tgacggtgta
    10561 ataaggtcgt gaactcatca atgaaattca catacccttg ctcaacttta cctaaacgta
    10621 aattcaaacg gttatatgcc attactgcag gaatcgccgc aaataaaccg atagccgttg
    10681 caattaatgc ttctgcaata cccggtgcga ctgattgtaa agttgcttgc ttaactgcac
    10741 ttaatcccat aaaggagtgc ataattcccc aaaccgttcc gaataaacca atatatgggc
    10801 tgattgaacc cactgtgcct aaaaacggaa tgtagttttc taaactttcc atttcacggt
    10861 ttaatgctaa gttcatcgca cgggtagaac cttgaataat cgattctggc gcatctgggt
    10921 tcgcttgttg taaacgggta aattctttaa acccaacata aaagatttgc tctgaaccat
    10981 ttaatgcatc acgacgattc tctaaacctt ggtgtaaacg gtttagatct tcccctgacc
    11041 agaatcgatc ttcaaaagca agggaatctt ttttggcttt agttaaaatt ttgctgcgct
    11101 gaataatcac cgcccaagac aaaattgaga atacaataag gatcagcatc acgatcttta
    11161 ccacaatact tgcttctaaa aataacgaaa gaagactaaa ttcagtttgc attaataaaa
    11221 ctccgaatag tttttaaata gaaaaataac ttttttgtaa tgcttgacga atatcatcag
    11281 gaattgcaac cggcttcatt ttgattaaat caacacaagc gaccgtaaca tttgcctcac
    11341 tcagacaatc gccatctttc cacaatcgtt gtttaaagat aagggttgct ttcttcaatt
    11401 ctgagagtgt tgtttccaca tttaataaat catctaaacg agcagggagc ttataatcta
    11461 tttcaatcct cttaacaaca aaagcaaggg attggctaaa aagttcctgc tgtgagaaat
    11521 gctgctgtct caaaaattcc gttcttgccc gctcaaaaaa attcagatag ttggaatgat
    11581 aaactactcc gccggcatca gtatcttcat aataaacacg aatcgggaaa tccataagca
    11641 ttatttctct cattcaaaat gaaaaaacgc ccaaaattta ggcgcaaatt gtgagatatt
    11701 ttaggatcag aaatattgtt atgcaagtat attttttggt aaattgcaaa aatatttttt
    11761 atctatcaaa aataaaaata gagacataaa aatgcctcta tttaacaagt aaaatccata
    11821 atgccggaat aacaataagg taacctagaa gtggtaaaaa aatcactttc caaatagcag
    11881 ctcggatttc aaatccaatt ccatgtaccc ataaaatagc cataccataa aataccaaca
    11941 ttgctaaata cggcgttttt ccaccaaaag aaagagcgaa agcggtcgaa tttacccaaa
    12001 tagttgcaaa taatacagcc gctaaaataa aagaaagggc ttttaacaag ccctttgctg
    12061 ttaagttata gagtttatga atcattactc gtcaaactta cctgtttttt caatgctgat
    12121 cgtgatgact gttgcaaaga agattgcaag caatacacct aagacccaag ttacataaaa
    12181 catattgtgt ctccttatta gtaagctgct ttattttctt ctaaataact agcatctaaa
    12241 cgaccataca ttttgatata tgaccaaata gtatatgcta atactaacgg tacaaagata
    12301 ccagcaacaa ataacattac agttaatgtt gtgtggctag cagtagcgtc ccaaaccgtt
    12361 aagctcattt ctggatgagt aattgacggc ataatgaatg ggaacattga tactgcagca
    12421 gttaaaatca ccccaacaat catcaatgat gatgagaaga atgctaaacc tgaacgattt
    12481 gcttttgagg caataatagt cagtaatgca cctactacaa ctaatgcagg aacaacccat
    12541 aatacaggca tttcagcata atttctaaac catgcaccag cttgaaccgc tacagtttta
    12601 ttcgtcaata gagattgtgc attatgatct aactcgcttg tgatcacaaa accatcttta
    12661 aaatataacc atacacctgc aagaataaat gccactaata caatagaagc tgtcacttgt
    12721 gccacttttc ttgagttgtt atgtagctca tcagtagttt tcatttgtaa ccaagttgca
    12781 ccctgtgtgg ttaacatcat taagctgata ataccgcaca ctaatgcaaa tggatttaat
    12841 aaaccaaaga atgaacccgt atattgtgat tggttaatat tattaaattc aaatggtaca
    12901 ccttgtaata agttaccgaa tgcaacaccg aatactaatg atggaacgaa accaccaata
    12961 aataatcccc aatcccaagc actacgccat ttcggattat caatttttgc acgatactca
    13021 aaacctacag ggcggaaaaa taatgctgca agcacaagca ccattgcaat atagaaaccc
    13081 gagaacgaag ttgcatagac tgttggccat gcagcgaaga tcgcaccacc agcggtcaat
    13141 aaccatactt ggttaccatc ccagtgaggg gcaattgtgt tgatcataat acgacgttca
    13201 acattacttt taccaatgat aggtaaaagg tttaatacgc ccatatcaaa accatcagta
    13261 attgcaaagc caattaacaa taccgcaatt aagatccacc aaataaagcg gagaatttca
    13321 taatcgatca tttctaactc ctattattta gttgattgtt caaaataata acggcctgtt
    13381 tttaatgcac tagggccaag acgaccatat ttgaacatta aatacatttc tacaactaag
    13441 aatagtgtat ataaaccaca caataagccg attgtaaacc ataaatcacc tgttgttaat
    13501 gcagagtttg acacaccgac aggtaacatt tcatagatag cccaaggctg acggccatat
    13561 tcggcaagga accaaccaga ttcaatcgca atccaaggta acggaagacc gaataacaag
    13621 cctcttaata agaaacggct actttctact ttaccacgca tattttgaat aaatgctgtc
    13681 gtaatcaaga taagcatcaa gaagccagat aacatcatta cacggaatgc ccagaatgta
    13741 gggcctacat ttggaattgt attatctgct gctttttgaa tttgttcttc agtcgcatca
    13801 accacattag gtgtatattg ttttaacaat aacccaaagc ctaaatcttt ttgtacagct
    13861 ttaaatgctt ctttatccgc ctctgtataa ttgccagaac gtaatttttc taatagtccg
    13921 taggctacca taccggtacg aacacgttgt tcattaatta cacgaatatc ttttaggcct
    13981 ttaatttctt tatctaaaga acgggttgca ataataccag ctgcataagg aatatgaatt
    14041 gagaattcat ttttcatttc cgcagtattt ggaatcacaa aagcattcca agcagctgga
    14101 gcaggctgag tttcgaattc accttccatt gcagctaatt tagtcggctg agctttaccg
    14161 atttcataac cagattcatc acccataatc agtacagaga tgatcgcaat taaaccaaaa
    14221 cttgcaccta ctgagaatga acgttttgca aagcctaagt cacgaccttt taagatatag
    14281 tatgaactaa tacctaatac aaaaatagca ccacaaacat agcctgcggt tacagtatgt
    14341 aagaatttag attgtgtaac tgggtttaat acaagttcag agaaacttga aagctccatt
    14401 cgcatggttt caaagttgaa ttcagaagca atcgggtttt gcatccaacc attagcaact
    14461 aaaatccata gtgcagagaa gttagaacca aacgcaacac agtaagttgc taataaatgt
    14521 ttcgctttcg ataaacgttc ccagccaaag aagaacaagc ccacaaaggt agattctaag
    14581 aagaacgcca ttaaaccttc aatcgctaat ggagcgccaa aaatatcacc tacataatga
    14641 gaataatatg accagttcat accaaactgg aattccattg taataccagt tgttacccct
    14701 agggcaaagt taataccaaa taatttaccc cagaatttag tcatatcttt atatacttct
    14761 ttacccgtag ttacatacag ggtttccatc accacaagga cgaaagaaag acctaatgta
    14821 agcggtacga ataagaagtg atagagtgca gttagagcaa actgcaaacg tgagag
//
LOCUS       DANLUA010000043        14843 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLUA010000043 DANLUA010000000
VERSION     DANLUA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            58..1476
                     /gene="leuC"
                     /locus_tag="PXB44_000969"
     CDS             58..1476
                     /gene="leuC"
                     /locus_tag="PXB44_000969"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL5281467.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1489..2091
                     /gene="leuD"
                     /locus_tag="PXB44_000970"
     CDS             1489..2091
                     /gene="leuD"
                     /locus_tag="PXB44_000970"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL5281468.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2163..2627)
                     /locus_tag="PXB44_000971"
     CDS             complement(2163..2627)
                     /locus_tag="PXB44_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281469.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2651..3157)
                     /locus_tag="PXB44_000972"
     CDS             complement(2651..3157)
                     /locus_tag="PXB44_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281470.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3180..4157)
                     /gene="dusA"
                     /locus_tag="PXB44_000973"
     CDS             complement(3180..4157)
                     /gene="dusA"
                     /locus_tag="PXB44_000973"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL5281471.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
     gene            4508..5485
                     /locus_tag="PXB44_000974"
     CDS             4508..5485
                     /locus_tag="PXB44_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5281472.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            5472..7139
                     /locus_tag="PXB44_000975"
     CDS             5472..7139
                     /locus_tag="PXB44_000975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5281473.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            7143..7562
                     /locus_tag="PXB44_000976"
     CDS             7143..7562
                     /locus_tag="PXB44_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281474.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(7797..8558)
                     /gene="nudC"
                     /locus_tag="PXB44_000977"
     CDS             complement(7797..8558)
                     /gene="nudC"
                     /locus_tag="PXB44_000977"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL5281475.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(8551..9234)
                     /locus_tag="PXB44_000978"
     CDS             complement(8551..9234)
                     /locus_tag="PXB44_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL5281476.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(9231..10079)
                     /locus_tag="PXB44_000979"
     CDS             complement(9231..10079)
                     /locus_tag="PXB44_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5281477.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(10079..10564)
                     /locus_tag="PXB44_000980"
     CDS             complement(10079..10564)
                     /locus_tag="PXB44_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL5281478.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(10566..11888)
                     /gene="pepQ"
                     /locus_tag="PXB44_000981"
     CDS             complement(10566..11888)
                     /gene="pepQ"
                     /locus_tag="PXB44_000981"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL5281479.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            11899..12141
                     /gene="tusA"
                     /locus_tag="PXB44_000982"
     CDS             11899..12141
                     /gene="tusA"
                     /locus_tag="PXB44_000982"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL5281480.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(12208..12639)
                     /gene="rraB"
                     /locus_tag="PXB44_000983"
     CDS             complement(12208..12639)
                     /gene="rraB"
                     /locus_tag="PXB44_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL5281481.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(12654..13265)
                     /locus_tag="PXB44_000984"
     CDS             complement(12654..13265)
                     /locus_tag="PXB44_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL5281482.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(13267..13683)
                     /locus_tag="PXB44_000985"
     CDS             complement(13267..13683)
                     /locus_tag="PXB44_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL5281483.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(13687..14517)
                     /locus_tag="PXB44_000986"
     CDS             complement(13687..14517)
                     /locus_tag="PXB44_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL5281484.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
BASE COUNT         4612 a         3230 c         2893 g         4108 t
ORIGIN      
        1 ttttttgcaa atttaaccgc ttgtattaaa agttaccctt ttgagttgga acattttatg
       61 caacaagcca aaaccctata tcaaaaacta ttcgactcac acgttgtgta tgaagccgaa
      121 ggcgaaacgc cgattattta tattaaccgc cacttaattc acgaagttac cagcccgcaa
      181 gcgtttgacg gcttgcgtgt tgccggtcgc caagttcgcc aagtcagtaa aaccttcggc
      241 acaatggacc acagtatttc aacccaagtg cgtgatgtac agcaacttga agggcaagcg
      301 aaaattcaag tattagaatt agaaaaaaac tgcgaagaaa ccggtatccc actgtttgac
      361 atcactaaaa aacaacaagg tatcgttcac gtaatggggc cggagcaagg cttaacttta
      421 cccggtatga ccattgtatg tggtgactcg cacaccgcca ctcacggggc ctttggtgcg
      481 ttagccttcg gtatcggtac ttccgaagtg gaacacgttt tagccaccca aaccttaaaa
      541 caggcccgtg caaaaagtat gaaagtggaa gtccgtggca aagtaaaccc gggcattacc
      601 gcaaaagata ttgtgcttgc gatcatcggt aaaacaacta tggctggcgg tacaggctat
      661 gtagtggaat tttgtggcga agcaattcgt gatctttcaa tggaagggcg aatgacagtg
      721 tgtaatatgg cgattgagtt tggtgcgaaa gccggtattg ttgccccgga tgaaaccacg
      781 ttcgcttact tgaaagaccg cccaaatgca ccaaaaggtc aagattggga agatgccatt
      841 gaatattgga aaaccctcaa agccgatgag ggtgcagttt acgatgcgga agtgatctta
      901 gaagcaaaag acattgctcc acaagtgact tggggaacca acccggggca ggtgatcggt
      961 attgatgatg taattccaaa tccggaagat atggccgatc ctgttactcg tgcctctgcg
     1021 gaaaaagcct tagcttacat cggcttagaa gcccatacag atatgaaaga tgtaccggtt
     1081 gatcaagtct ttattggttc ttgtactaat gctcgtattg aagatttgcg tgctgcggca
     1141 gcagtaatga aagggcgtaa aaaagccgac aatgtaaaac gtattctcgt tgtgccaggt
     1201 tcaggtttgg taaaagagca agcagaaaaa gagggcttgg ataaaatctt cattgaagcc
     1261 ggtgcagaat ggcgtaaccc gggttgctca atgtgtttgg gaatgaacga tgaccgcttg
     1321 ggcgaatggg aacgttgtgc ctctacgtct aaccgtaact ttgagggcag acaaggacgt
     1381 aacggtcgta cccacttagt cagcccggca atggcggctg cggcggcggt gttcggtaaa
     1441 tttatggata ttcgcaaagt gtcattaaac gcataatagg aggaaaaaat ggcaggtatt
     1501 aaacaacatt caggcttggt cgttcctctt gatgcagcga acgtagacac cgatgcaatc
     1561 attccaaaac aatttttgca agccattact cgagtcggct ttggtaagca ccttttccac
     1621 gaatggcgtt atttagatgc ggaagagact caaatcaacc ccgatttcgt actgaacttc
     1681 ccacaatatc aaggtgcaac cattttactc gcccgtaaaa atttaggctg tggctcttct
     1741 cgtgagcacg ccccttgggc attggcagat tacggtttta aagtaatgat cgccccaagt
     1801 tttgcggata ttttctacaa taacagctta aataaccata tgttgccgat taaattaacc
     1861 gaagaggaag tcgaagagat cttccaatgg gtgtgggcaa atgaaggaaa acagatccac
     1921 gttgatttag aagcgatgac tgtgactgtt ggcgagaaag tttatacctt tgagttagac
     1981 gaattccgcc gccactgctt attaaacggt ttggataata tcggcttaac cctacaacac
     2041 gaagctgcga ttgctgctta tgagaaaaac attccggcat ttttacgcta attaaaatac
     2101 aaaaaaacgg ctaacatctt taaggcgtta gtcgttttct ttttagccat tttctttggc
     2161 aattagcctt ctcggcactc taaccaaggc acattatttt tgtaataagt tgaagtaaac
     2221 tctcgtgcca ctaactctac aaaactgcct tcaatcggct gataagaacg ataacgcact
     2281 tcaaattttg ttgctcttgc attcaaactg atgttggcaa gatggtcgaa cggataaacc
     2341 tttccgccat caagttgaaa atagatacca aaattctctt gcttacggct ttcagaccaa
     2401 agtggaaaat aggttgccaa atatgaactg ctgcctgttg caaaattctt acaataatag
     2461 ctataacgct taaaatcttt cggattattc aattccgctt ggctgatatt ctgtttcaaa
     2521 tatccaacct gtgcttgttg cttagttgag tttgattgtg gtgagggcgt acaagcaaat
     2581 aagccaattg ccactgttgc cattaaagca attttgaatt tattcataaa aatcccgata
     2641 aaatattaaa ttaaatctga tatttgcttt tatcgccaat atcaaaatgc agcggcattc
     2701 gccatttttg aatactcagc acaagaagca acaagccaca gacaaggctt aaaatttgca
     2761 ccaataaaga ccattctagt gcaaaagcaa tccacaatac cgccactaaa agcggttgga
     2821 aatttaagcc gaatactcta aaacagaaat attctttata gcttaatccg ccgatcacca
     2881 gtagcattgc cccgaacgca agcaaaggga aattaaacac agcaccaagt aagccaatcc
     2941 aaacagaaaa ttggaaaatc aggcgaaacg atttggcata aagatgcaaa gaagatgcca
     3001 acattgtgcc agatactaat aaaccatatt gtgccgtttg cggataataa ggcaataaaa
     3061 gcaacatcaa tgtagctaac acaaaaccgc tgcggtaaat gatgacggtt aaataatccc
     3121 aaaaatccat tggcgattga atatgtggat ctgccataac tgcctcctta aacaataaat
     3181 tagccaataa acgcaagggc ttgttccact acttctacac cggctcccga cttagtggca
     3241 ttttcactta aatgacgacg ccactgcctc gctcctttgc aatgttgaaa cgcaccaagc
     3301 atatggcgaa cgatatgatt taaatagaca cctttggcaa gctcacgttc aatataaggg
     3361 aacatctttt ccaccgcttc ttttgccgtt acaagcggtc gattttcatt aaaaatttgc
     3421 gaatcgatct cgcctagcat tgacgggttt tgataggcct cacgccctac cattacccca
     3481 tcaacaaact gcaaatgctg tttaatttcc tcaatggttt taatgccgcc attgatgcta
     3541 atctttaaat gcggaaaatc tcgcttgagc tgataaaccc gatcataatc taaaggcgga
     3601 atttcacggt tttccttcgg gcttaaccct gtcagccacg ccttgcgggc gtgtacgata
     3661 aaatcattgc tataaggttg cactttttcg ataaaatcac acaaaaattc atagctgtct
     3721 aaatcatcaa tcccaatacg atgtttaacc gtgaccggaa tttccaccgc atcttgcatt
     3781 gctttaatac aatccgccac taaatctgct tttgccatca aacaagcccc aaacataccg
     3841 ttttgcactc ggtctgacgg gcaaccgaca tttagattaa tctcggcata gcctcgctct
     3901 gccaccaatt tggcacaatg agccaactgt gccgggtcgc ttccacccag ttgcaaagcc
     3961 accggattct cgctcggatc atattccaac aaatcatatt tggcgtgaat aatcgccggg
     4021 gcggtgatca tttcggtata aagcaaggct tgacgactaa attggcgatg aaaatagcgg
     4081 cagtgacggg ttgtccaatc taacattgga gcaaccgaaa aacgtccacg gtaaaaaggt
     4141 tgaatttcgt tagtcataaa aatatcaaaa taaagcggta aaaaatcagt aaaaaataac
     4201 cgcttgtaag cgattcaaaa caagcagcca tcatactcga aaaagccaga taaacatagt
     4261 ttcctcaagg cttccccatc tatctgcgca cttttaaaac aaccttaaaa cacaatatat
     4321 agtgtttcaa catcaaatca cacacaacat attgaagcaa aaatttacaa aattattttt
     4381 ctttataaat caataagcta ccaaagacca ataaaaaaga gtgtttgtca agacttgtct
     4441 tgaaactttt tttgcataca atcatctacc agttccaaac ataaacttta agatagagcc
     4501 tacgaatatg tctagaaacc tcttccaaaa acgcttaaat attaagccgt atgagtatcc
     4561 tgagcttctt gaatttaaag atgcaattcg ccattcttac tggttacaca ccgaatttaa
     4621 cttcaccggc gatattcaag actatcgcac caccattaac gatcacgaac gtcacgtgtt
     4681 aacccgagca atgcttgcga tttcacaagt agaagtgaat gtaaaacgtt tctggggcga
     4741 gctatactgc tatttcccaa aaccggagat ggacgatgta ggcggtactt ttgctgaaag
     4801 tgaagttcgc cataaagatg cttactcgtt cctattggaa aaattaggcc taaatgaaat
     4861 gttcagtcaa attcatgaaa ttgaaccatt aatgaaccgc attcgttata tggaaagttt
     4921 tatgaaagat aaagatgctg gcaaaggcga atttgtttta tcgttagtgc tgttctcttt
     4981 atttgttgag catatttcat tgtttggaca gtttattatc atgatgtcgt tcaacaagca
     5041 taaaaacctg tttaaaggga tttccaatgc ggttgaagcc acctcaaaag aggaagaaat
     5101 tcacggtaaa ttcggaattg cgttatacga aattttacgt gacgaacaca gtgaactatt
     5161 cacaccagaa ttttttgaag atttgcaaac cttggcaaat caagcgtttg aagcagaacg
     5221 tggcatctta gactggattt tcgaagaagg tgatttaagc tttatcacca aagcgacggt
     5281 agaaaattac attaaaaacc gttacaacaa ttctttaagc attttagggt tagagccgcc
     5341 acacgacatc gaccctgaat tattaaaaga gactgagtgg tttgatattg aaattctttc
     5401 aaccaaagaa accgatttct ttaacaaacg tagcaccgat tattctaaaa aaatgaaaca
     5461 gattaccgca gatgacttat tttaatacag accgcccgga cttccactgg cttaatgaag
     5521 acagccgttt attcttacaa cgtggctatt tattggaagg aacaaccgca ttagaccgca
     5581 ttcgttttat tgccgaacac gcagagcgaa aactcggcat tgaaggctat gccgataaat
     5641 tctatcacta tatggcaaga ggctacttct cgctttcttc gccaatttgg tcaaactttg
     5701 gcttagatcg tggcttaccg atttcttgtt tcggtagcta catcggcgac tctatccatg
     5761 aaattatggt gactaccgct gaagtgggca tgatgagtaa aattggcggt ggtacttcgg
     5821 cctactttgg cgatattcgt ccgcgtggct cgttaattaa aaacaacggt aaatcagacg
     5881 gctcatttaa cttcagtaaa ttatttgaca ctgtgattga cgtgatttca caaggaacat
     5941 cacgcaaagg tcagtttgcc ggttatattg atattgagca cggcgatatt gacgaatggc
     6001 tggatattca caccgaaggc aacccaattc aattaatgta ttacggtgtg tgtgtcggtc
     6061 acgattggtt agagtcaatg aaagccggcg atccatacaa acgtcagctt tgggcaaaat
     6121 tgttacaacg taaaaccgaa accggtatcc cttatttatt ctttaaagac aatgccaacg
     6181 caggtcgtcc tgatgtatat aaagataaaa atatgacgat tcacgcctct aacttatgta
     6241 ctgaaattat gttgccttcc agcaacgaag aaagtttcgt atgctgttta tcgtctatga
     6301 acctactcta ctttgatgag tggaaagata ccgatgcacc agaaacatta acctatttcc
     6361 tcgatgttgt aatgagtgaa tttattgaga aaagtcaatc aattccattc ttagaccgtg
     6421 caaaccgctt tgcccgtcgc catcgtgcat taggtttagg cgtgttgggc tggcacagct
     6481 atctacaggc aaataatatt gcctttgaca gcttccaagc gatgcaaaaa aacaacgaga
     6541 ttttcaaaac gcttcaagca aaaaccttga aagcctcgca agaattggct caacgcttcg
     6601 gcgagccgga aattttagag ggctatggtc gccgtaatac tacattaatg agtattgctc
     6661 cgactaaatc cagtagcttt attttaggca gtgtgtcgcc atcggtagag ccgtttaaat
     6721 ccaactacta tgtaaaagat ttagcgaaga tcaaaaccgt gtataaaaac ccgttcttag
     6781 agaaattact ccaagaaaaa gggctagaca ccgaagaaat ttggacgtct attttacaaa
     6841 atgatggctc ggttcagcat ttggaacagc tttccgagca cgaaaaagaa gtatttaaaa
     6901 cctttgccga aatcagccaa ttaagcgtga ttcagcaagc tgctcaacgt caaaaatata
     6961 ttgaccaagg gcaaagtatt aatgtgatga tccacccagc cacaccggct cgtgatctta
     7021 accaattata cttaacagca gaagagctgg gcttaaaatc catctactac caaaactcaa
     7081 tgagtgcagc acaggtattt aaccgtaacc tattaagctg ctcaagttgc gaagggtaaa
     7141 taatgggaaa tttatcgcat acttcaccta atactattgt tgaagttgtt ggtgaatttc
     7201 ctgaaataac atctagtggg tatacctttg ataaaggtcc tattcaatta agaatcggca
     7261 aacatactgc tccaaataaa ggctttggtg taaagcatat tgaagccgag catatgcaag
     7321 aaattcttaa tctaggattt acatcagtta atgaatttgt caaagctatc atagtagaaa
     7381 acgctaaaat tttttgcgaa ttctcagatt taaaaggcaa tcatcgccct attattctga
     7441 aagcaagtat tggcattgtg gttctagagc gtaagttttt ggaaagcggt acgatatatt
     7501 cagttgtaac cgcttataga agaacaaatc ctcacggaat acaaataggc actatgaaat
     7561 aaagtacgtt agtgttataa tcagttccac gcaagagcat ttgtagaaaa taacaggcgg
     7621 atttggtttt ctactgtggg cggcaggttt ccgctaacct gtaatagcta tttaattttt
     7681 atattaaatc ctgaacttga aacagactaa gggattgctg cggtagctaa gaatacaaac
     7741 ctctctataa tcatatagag aggttttttc ttattgatga gcacaagtta agataattat
     7801 ttactctccc gacataactt caaggtagct tgaatcagct caaaagctat tgtgccttgt
     7861 tgtgggattt caggtaagct ttcctcacag tcgaaccatt ttgcatcgca gatttcttct
     7921 tcctgtagct gaatttcacc gctttcataa tccgctaaaa aacctagcat taatgaattg
     7981 ggaaacgccc aaggttggct tccaaaataa cgcacatttt tcactttaat gcccgtttct
     8041 tcaaacactt cacgatgcac cgcagtttct accgcctcac cggcttcgac aaaaccggct
     8101 aaagtggtat aaattgtgcc tttatggcgt agatgattgg ctagtaggat ttgtctgcct
     8161 tttcgcaccg ccacaataat tgacgggcta atcgttgggt aagctcgatt gccacacgca
     8221 ttgcagtaaa ttgcaatttc atcgcttgcc agtatggttt tctcgccaca ttttccgcaa
     8281 aattggtgag tctgaaagaa atggttaagc gaaacagctc gatgcaacaa atgagacagt
     8341 tcgggtggtc ggttaatttg gcttcttaaa taagcaaact ctcgctgatt atccgattct
     8401 aacgccttca ccaaataagc cggcgagcct tgatattgcc cgatggttaa tttctcacgt
     8461 ccacttaagc ccagctcttc cgcacttccg aatggaattt ccccagcctc caaggcaata
     8521 tcaaaatcgc tgacgataat ccaatatcct ttattcatct ttcaatccta attgtgtaaa
     8581 caacttacgt ttttcatttt cagatagcac ttgaaactca ccacagccta aattgcctaa
     8641 tctaaactgg cctatcccaa cacgaattaa ccgtaaagtc gggaaaccga tatgtgcagt
     8701 cattcttctg acttggcgat ttttcccttc agaaataatc aattctatcc agctggtcgg
     8761 aatggttttc cgctcacgaa tttgcggggc gatattccaa gcaaaatcgg gcgttgctat
     8821 tgctctgact ttcgcgggct tggtcaaacc atcttttaac tccacccctt ttgctaattt
     8881 ggcaagatct tccggttgtg gaacaccctc cacttgtacc caataggttt tctgcttgtc
     8941 aaatttcggg tgagctaaac ggtgctggat ttcgccatta ttagtcaaaa gcaacaatcc
     9001 ttcactgtct ctatctagcc gcccgaccgg gtaaatattg ggcatcgtaa taaaatcttt
     9061 tagcgttttt cgcccctgtt catcggtaaa ttgggttagt acatcataag gcttattgag
     9121 taacacaagg gtggtttccg caaaggtaag cggtcgtttt tgcggatttt tttgcaaatt
     9181 tttagcgaaa tttgaccgct tgtttgtcat tcttttaaag gttggttgtt tcattttttc
     9241 aggttcgccc acgcaacaga aagtaaaata attaagccac cgataatcat tttaagggtt
     9301 ggcacttcat taaagcaaag ccacgcgatc acaatcgcat acaccggctc gagggaaata
     9361 atcattgagg cagtacgggc gttgatggta tcaaggcttg aaataaacag cgtataggca
     9421 agggttgtac ataaaaaacc gatacagaaa atccaaaacc aatcttgggt ggaaacgctt
     9481 ggaaattcag tgatggagag aggcaacaga aaaagtgtag caaaaatata ctgccaccag
     9541 cttgcttgca caccggaaat tttgctcatg gtacggcgat tcgctaccgc caaaatacca
     9601 tacaccaaag cagataacac gccccacaat aaaccttgag tggcttgatt accaaaagta
     9661 aattcagggg taactaaaat taaaccaacc gtgatggcaa ttaatagcac cacttcacga
     9721 aagcgtaatt tttcacggaa gaacaggctt tcaaataacg ccacaaatgc cggaaagctg
     9781 gcaaagccta aggttgctac cgccacacca cctactttaa ccgccacaaa aaaagtaacc
     9841 cagtgggcag ctaataaagc tgcagaaaat gcaagttgag ccatttgctg taagttgagc
     9901 tttacaagcg gttgtttttt ccataaaaaa tacaaagaga tgatagcaaa agcaatcatc
     9961 actcgcccaa gcacaatggt ttcagccgag ctttgaatta atgctccgaa gatccccgag
    10021 gcaccaaata aaaccgctgt gccgtgtact ttaagtagtg caagtgttct tttctccact
    10081 atttgatcct attatgttga tcttgctcgt ccggcttgcg ttcaaattct gcatcaaacg
    10141 tgttgctatc ttgcgcagat gtaacagaaa aagacgaagt ttgggcggta aaatgcataa
    10201 acaccttatt ctttacgcta ttaattacga aggcttgaat ccattttcgg ctaaagggta
    10261 acacgcataa aatcgctaaa atatcactta aaaagccggg aataatcagc aaaatgccgg
    10321 ctacaataaa catcaccgag ttgccgactg cttcagtcgg tatcttgcct tggcttaaat
    10381 cttgcttgat ggaaaagagg gtataaacgc cccttaactt cacaatccat aaccctaaaa
    10441 atgaaatcac gaccagtaac aaaatcgtgg gtaacacgcc aatgatcgag ccaatattca
    10501 ccagcagcga aatttcgcaa tagatataaa agaaaatacc aaacagaatc gctaaaatag
    10561 gcatattatc ctcccaacgt tgcggcagcg tttgccgtca gattttctgc cccattggct
    10621 cgcatgacaa tattgtcttc ggtacgaatc ccgccaaagg ctttgaaagc ctcaattttc
    10681 tgccagttaa aggcagaaga taaggcactg tttttccaag gcttaagtag catctcaata
    10741 aaataaaaac ccggctcaat ggtaagcacc atattttcct gtagctcacg ggtacaacgc
    10801 aaacttgggt aaacctctgg aggagcttta tggctgcctc gggcattttg ctgaaaaccg
    10861 cccacatcgt gcacttgaat acccaataaa tgccctagtc cgtgtgggaa gaacgtacgg
    10921 ctaatccctt gttcaaaaat ttgctcggct gaaagatgaa taaattgatg ttccgctaat
    10981 aattgcgaaa tcatctgctg cattcgggtg tgataatgca ggtagttcac cccaatactc
    11041 agctcagcaa taatctgcaa cttcaactgt tccattttcg ccaccagctc ggcaaattca
    11101 gaaccgctgt caaaggcata ggtgcgggtg agatcggagg cataaccatt taccgttgtg
    11161 ccggcatcaa ttaaaaaact gtggcgtttt gcgtgggtgt caaaatctaa cgccgtataa
    11221 tgtagcaccg caccattttg attaatcgcc acaatattgc cataaggaac attgagatca
    11281 gattgctgcg tcgctttcaa atattcaagg ttgatttcaa attcgctttt gccggcaaaa
    11341 aatgcctctt ttgctacttg atgccctttt aaggctgaaa attgggcttg gtaaatactt
    11401 tcaatttcaa actccgtttt gatggagcgg gcaaaattca ggctattcag cactttctgc
    11461 ggattaatat ggctaaagcc taactttttg gctagctcaa catcttcacc gataaaggca
    11521 caagcggtcg gattttggat aaaggttgca atttcattct ctgctctcaa caaaacccac
    11581 tcaaattccg cagcgaaaaa ggcctccgtt ggcactttta gagtggaaac ccaataatct
    11641 tcaggggcat aaaaatagat tttcggctta tttttgccat ctaaaaataa ccagcaattt
    11701 tcagcttggg gatatgggaa aaagtaatta aaataagggt taattttaaa cggggcggtt
    11761 tgatcgtcta aaaataaggt ttttgccttg ccggcataaa tccataaacc gtctaaaaag
    11821 gcattttcca gcaagctttg cactgtctgt tgcaaacgtg cgagatgcgt agagaataga
    11881 tttttcataa ggaataaaat gactcaatta actgtaaata aagaattaga taccttgggg
    11941 ctacgttgcc cagagccggt gatgctcacc cgtaaaacaa tccgtaatat ggaagaggga
    12001 gaaattttgc atatcatcgc cgatgacccg gccactaccc gtgatattcc gagtttctgc
    12061 gaatttatgg atcaccaatt aattcaaaaa gaggtggact ccaccccata ccattactgg
    12121 attaaaaaag gcttggaata aaccacaagc ggtcgatttt agccataaaa ttgcaaattt
    12181 atgcacaaaa tcgaccgctt gttttaatta atgacggcga ggttcgtcct caaaatcgtc
    12241 attttcatca tcgaaaaatt caccgtcatc gccgtactca tcttcatcag cattcggatc
    12301 ttcaaagtag gttccccagc cctcatactc cgcaccggct ttttcaatta atggcagtaa
    12361 ctctttttgt tgtgcggtaa tgagatccgc attcaagtga atttcgctca caatgtcgca
    12421 tacaaaaatc actttaccat tttcgtcttc cacttcttcc gcttcggaaa tttcatagcc
    12481 cagcttatag gcatcaacta ctaatttttc caatttatca aaatcgtggt gagcaacgtg
    12541 gtgttcaata atataaaggg cttcagggtc tgaaccatcg gctaataatg atgaaatggt
    12601 ttcatcggtt tctgtgcgta attcatcgag attgtacata ttgaattcct tacttattct
    12661 gtgattaatt ttggtgcaaa ccagctatct aatttgttcg acagcttatc aataccgatt
    12721 ttattcagtg cggaataagc ctctacttgg atatcacctt ggaatggtaa aatcgcttct
    12781 cttaccattt ttacctgctt agagcgtgcc gattggctta acttatccgc tttggtcaac
    12841 agcagtaaca ccggcaagtt ggctgacact gcccattcaa tcatctgttg atctaaatct
    12901 ttcaacgggt ggcgaatatc cattaaaatc accacaccgc ctaaacattc acgcttttgt
    12961 aaatactcgc ctaatgcttt ttgccactgg attttcatct gttcaggtac tgctgcatag
    13021 ccatagccag gtaaatccac taatttacaa ttcgtttcaa ccttaaacaa gttaatcaac
    13081 tgcgttcgcc ccggtgtttt ggaagttcgg gctaaacttt tttggttagt taaagcgttc
    13141 aaagcagtcg atttaccggc atttgaacgc ccggcaaaag caatctccac acctgtatct
    13201 tcaggtaaat gacgaatatc cggtgctgaa gttaaaaaat gggttttatg atagtttaat
    13261 ttcattttat tctctaaatt taaaagataa gccttgctcg ccatattgat tcggttcagg
    13321 attaccggca aatacacccc attctaaaaa gagcaacatt ccaataaatg ccggcatcac
    13381 ctgtccaagc aatatctgca taattccttg tgaataagga gctgcaagat aacaaataat
    13441 cggcactaat gccattaaca gggcttttgc cgaacgattt cggtcgtgta atcgtttaat
    13501 aataatagca gacaggctaa atcctgtaat tataagcggt agcacactca ataaagacaa
    13561 ttcagccaaa tttatccaca tagcaaccat aaacaggaaa agaaagtgga ttccaaatcc
    13621 aatccaaaaa cctcgtctat ttagcctacc gttaaaacta aaaagtgctt tataccaact
    13681 cattttctac cctaaagcta catcaagtac catcattact acaaagccta acatcaggct
    13741 taaagtcgca atatcagtat ttcccgaact ttgcgattcc ggaatcagtt cttctacgac
    13801 cacaaaaatc atcgctccgg cagcaaaggc aagagcataa ggtaaaatag ccgtcatcga
    13861 cagcacaaaa accgccccaa tcaccgccgc aataggctca accactgctg acatagagcc
    13921 ataccaaaat gctttcttcc gactatgccc ttcgcctcga attggcaata caagtgacga
    13981 gccttcggga atgttttgta accctattcc gattgccaaa ccgatagctc ccataatgga
    14041 agcatctacg cctgaaactt gggttgccaa agcaccgaaa gccaccccta tggctaatcc
    14101 ttctggaata ttatgaatgg taatcgcaag aaaaagcaaa gtagattttg ataagccttg
    14161 tttaagttga tcatttcctt cagcttgctc aatcggctta ctcaagtgca aatgcggcac
    14221 aacatagtca attaatcgaa taaaagctcc accggcaaga aagccaatcg ctgcaggcag
    14281 ccaagctaaa gagccataat ccgcctcggc atattctaaa gaaggtgcaa gcaaagacca
    14341 aaacgaggct gcaatcatta cacctgcggc aaagcccatc atactgtcta ataattttcg
    14401 attaaccgat ttaaaaaaat agacaaatga cgaaccgaaa atcgtacaac cccaagtaaa
    14461 taaacccgct aaaaaagctt gccatattgg agaaagtgaa aggaaatatt caaacaagaa
    14521 atagtaccag ctaaaataaa tattggctca ttctatcaaa gattcagggt tattaatata
    14581 gtgtagccaa aggctcaacg aagatttcac ttgtaaaact tagcaagaaa ataaccgctt
    14641 gcatttccct ttcataaata caatgaaaat cctaacaagc ggtcattttc cgtgagtttt
    14701 ttacctttac cacaacaaca aaaaaccgcc tgtatctcta caggcggctc tttcttggat
    14761 tacttgtact ttgtttattt gtacttattt tgcttttgct taagttttcg cttttgtaac
    14821 ttttagcttt cgcttaagct cgc
//
LOCUS       DANLUA010000044        14786 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLUA010000044 DANLUA010000000
VERSION     DANLUA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14786
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(1..489)
                     /gene="sixA"
                     /locus_tag="PXB44_000987"
     CDS             complement(1..489)
                     /gene="sixA"
                     /locus_tag="PXB44_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL5281485.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            616..2055
                     /gene="pyk"
                     /locus_tag="PXB44_000988"
     CDS             616..2055
                     /gene="pyk"
                     /locus_tag="PXB44_000988"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5281486.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            2151..3188
                     /locus_tag="PXB44_000989"
     CDS             2151..3188
                     /locus_tag="PXB44_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5281487.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            3228..4286
                     /locus_tag="PXB44_000990"
     CDS             3228..4286
                     /locus_tag="PXB44_000990"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL5281488.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            4341..5462
                     /gene="tyrA"
                     /locus_tag="PXB44_000991"
     CDS             4341..5462
                     /gene="tyrA"
                     /locus_tag="PXB44_000991"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL5281489.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(5653..6738)
                     /gene="recF"
                     /locus_tag="PXB44_000992"
     CDS             complement(5653..6738)
                     /gene="recF"
                     /locus_tag="PXB44_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL5281490.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            6878..7207
                     /locus_tag="PXB44_000993"
     CDS             6878..7207
                     /locus_tag="PXB44_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL5281491.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            7215..7820
                     /gene="recR"
                     /locus_tag="PXB44_000994"
     CDS             7215..7820
                     /gene="recR"
                     /locus_tag="PXB44_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL5281492.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            7892..8218
                     /gene="grxD"
                     /locus_tag="PXB44_000995"
     CDS             7892..8218
                     /gene="grxD"
                     /locus_tag="PXB44_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL5281493.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            8360..9397
                     /locus_tag="PXB44_000996"
     CDS             8360..9397
                     /locus_tag="PXB44_000996"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL5281494.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            9424..9972
                     /locus_tag="PXB44_000997"
     CDS             9424..9972
                     /locus_tag="PXB44_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL5281495.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            9981..10325
                     /locus_tag="PXB44_000998"
     CDS             9981..10325
                     /locus_tag="PXB44_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5281496.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(10358..12136)
                     /locus_tag="PXB44_000999"
     CDS             complement(10358..12136)
                     /locus_tag="PXB44_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL5281497.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(12362..12931)
                     /locus_tag="PXB44_001000"
     CDS             complement(12362..12931)
                     /locus_tag="PXB44_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL5281498.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(12941..14704)
                     /locus_tag="PXB44_001001"
     CDS             complement(12941..14704)
                     /locus_tag="PXB44_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL5281499.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIVVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         4447 a         2855 c         3199 g         4285 t
ORIGIN      
        1 ttaagtatgc tttactcgaa ttagctcagc ttttgtcttt acccaattaa tttctgctat
       61 ggttgaagtt ggaaattgta cattgctttg gttattcgtt aaaatttgga cgagatcgaa
      121 cacaagtggg agatgtgaaa tcagaagaat attttttgcg ccttcctctc ttaaaaaatg
      181 tagatagctt tctactgtat gcgggtttcc gtcaggcgta acctcgtccc aactttcaat
      241 gaggttggta aaatgttggg gaaaattgac cgcttgcata ccaataagca cattttcaag
      301 cgtttgttgc gccctaaggt atgggctgac gagaatttta tctaattgaa tttgttggtt
      361 ggctaaatgt tctcctaacc attttccctg tgaaatggtg ttttttatac cattgtcggt
      421 taaacagcga gcagcatcac tcgaagcatt aaagcctgct tccccatggc gcataatcca
      481 aatattcata acattctcct ttagttgtat ttaaactctc ttgatttagg cgtataatga
      541 ccctcgttta acgcataatc aagatgctta gaaagatttt aacttaattt ttaaatttat
      601 tgatggagtc aaataatgtc tagaaaactc agaagaacga agatcgtttg tacaatggga
      661 cctgcaacag atcgtggcaa tacgttggaa aaaatcattg ctgccggtgc aaatatggtt
      721 cgtatgaatt tctctcacgg tattcctgaa gatcacatcg aacgtgcgaa taaagtgcgt
      781 gaaattgctg caagattagg tcgccacgtt gcgattttag gcgatttgca aggtcctaaa
      841 attcgtgttt cgacatttaa agacggtaaa attttcttaa atattggtga taaattcacc
      901 ttagacgcag atttaccgcg tggagaaggc catcaagaag ctgtaggttt agattacaaa
      961 aacttaccaa atgatgtagt acctggtgat attctattgt tagatgacgg taatgttcaa
     1021 ctgaaagtgt tggaagtgga aggcgttaaa gttcatactg aagtgacagt tggtggcccg
     1081 ttatcaaaca ataaagggat taacaaatta ggtggtggtt tatcagctcc tgcattaacc
     1141 gacaaagata aagaagacat taaattagcg gcgaaaatcg gtgtggatta cctagcggtt
     1201 tcattccctc aatcaagtgc agatttacat tatgcacgtg aattagcaaa agaagcaggc
     1261 ttagaagcga aaattgtagc gaaggttgaa cgtgccgaaa ctgttgcgac tgaagaagca
     1321 atggacgaca tcattttagg tgcggacgtt gtgatggttg ctcgtggtga cttaggggtt
     1381 gaaattggtg atgcggcttt agtgggtgtg caaaaacgtt taatccgccg tgctcgtaag
     1441 ctaaatcgtg tggtaattac cgcaacccaa atgatggaat cgatgattaa aaaaccaatg
     1501 ccgacccgtg ctgaagtgat ggatgttgca aatgctgtgt tagatggtac tgatgcggta
     1561 atgctttccg gcgaaactgc aaacggtgac taccctgttg aaacggtaaa atcaatggca
     1621 gaagtgtgct taggtgctga aacaatgcca agcattaaca tttctcgcca ccgtatggaa
     1681 ggtacgttta aatctatcga agaagctgtg gcaatgtctg caatgtacac cgcaaaccat
     1741 ttagaaggtg tatcggcaat tatttcatta actcattcag gcgaaacggc taaattaacc
     1801 agccgtatca gctcaggttt accgatttat gcgatgtctc gtaaccaaaa agcattaaac
     1861 ctttgtgcgt tatatcgtgg tgtaacaccg gtatattaca atgaagacag ccgcacaatg
     1921 gaaggggcga aaaaagccat tgctcaatta aaagaacaag gctatttatt aaccggcgaa
     1981 ttagtgcttt taactcacgg agatgaatta aaagctggcg gtacaaatac ttgtcgtatc
     2041 ttaagagttg aataatctaa acgattaacg caaaagccac ataaaacact ttatgtggct
     2101 ttttttctgt cttaatatag cttaatgtaa tctgaactat tgatattatt atgcacaata
     2161 aacttctttc tatctgtatt ctccgccttt ctgccattgg cgacgtttgc cataccttag
     2221 cggttattca agctattcaa cgaaaatatc ctagtgctga aattacttgg attatcggca
     2281 aaacggaagc gtatttaatg aaagatattc cgaatgttca cttaattcct tatgataaaa
     2341 aaatagggtg gaaaggtatc tttacacttt ggcaaaaact ttctcacaag cggtttgatt
     2401 ttttattaaa tttacaaacg gcattccgag cttcaattct atcgcttggg attaaagcaa
     2461 agaaaaaaat cggctttaat caagacagag caagggaaat gcaatggttg tttaccaacc
     2521 agaaagtaga aatgacagac tctcctcacg tgcttgacgg gcaaatgatg tttgccaaag
     2581 ccattgggat aatggattta tcggcacaat ggaatttacc tgtgagtgat gaagatttag
     2641 cttatggcaa acaatttatt gattttacaa ggaaaaatat actcattgct ccttgttcaa
     2701 gcaagaaaga gaaagattgg ttgccggaac gctatgcaga aattgctaac tggctaagta
     2761 agcaaaatat caatgtatta attgcaggtt ctccatcgca atatgaaatg gaaaccgcag
     2821 ccaaaattca acaacttgcc ccgaattgta cgagtattgc cggtaaaacc acgctaaaac
     2881 agcttgcagg tgttattcgt caggttgatt tggtgatctc ttcagattca ggtcctgccc
     2941 atatcgccac tacgcaaaat acgccggtga ttgggttgta tgcgattcat aatccaagac
     3001 ggacaggccc ttacaatgac ttagacaaag tggtttcggt atatgatgaa gcgattttgc
     3061 aatcttatgg taaaccttgg cagcaattag cttgggcaac taaagctaaa ggcaaaaatt
     3121 ggatggaaaa aatcacggtg gagagtgtga aacaaaaagt agttgaaact ttaaaaatta
     3181 cgttgtaaag tttgtgtatt aaaagattta cacaaaagga aattaaaatg aacacagtgt
     3241 ttaatcaaga tagcgtgcat aacgtaaata tccaagatga aaaggtatta ctgaccccac
     3301 aagagttaaa acaagaattt ccgttgccgg aacatttacg caatcaaatt gaacgttcac
     3361 gcaaaacgat ttccgatatt attcacagac gagataaacg ccaactgatt gtgattggac
     3421 cttgttcgat tcacgaccca gttgctgccc ttgaatatgg taaaaaatta aaagctcttt
     3481 cggagcaagt gagtgataag ttgtatattg tgatgcgggt ttattttgaa aagccccgca
     3541 ctactgtagg ttggaaaggg ctgattaacg atccgaaaat ggacggctct tttgatgtcg
     3601 aaaccggctt acgtatcgca cgaaaactct gtttggattt agccgaactt ggcttgccgt
     3661 tagcgaccga agccttagat ccgatgacac cacaatattt agcggatttg ttcagctggt
     3721 cggcaattgg agcaagaaca actgagtctc aaactcaccg tgaattagcc tcaggtttgt
     3781 caatggcggt aggctttaaa aatggtacag atggtgattt agccgttgca cttaatgcga
     3841 tgcaatcttc cgctcaaagc catagcttta tcggcattaa ccaacagggg caagttactt
     3901 tactcaaaac aaaaggtaat gcagacggac atattatttt acgtggcggt aaaaccccaa
     3961 attatgaaaa gcaatatgtt gatgattgtg aatgggtgct aaaacaagca aacttaccta
     4021 cagcgattat gattgattgc agccacggca attccaataa agattaccgc cgccaaccgc
     4081 tggtggcaga aaatgtatta gagcagctac ttgctggcaa tacttcgatt atcggtttaa
     4141 tgattgaaag caatttacac gcaggtaatc aatcatcaga attgccgttc gagcagatga
     4201 aatacggtgt ttctattacg gatgcttgta ttgactggca aactacagaa gagcttttaa
     4261 ctcactttgc tgaaaagttg cgttaaagat aatttgcaaa aaattgcata aaaacgaccg
     4321 cttgtaaatt ggtaacaaat atgaacccat tagccccatt acgagaacaa attgatcaag
     4381 ttgatcaaca acttatcgcc ttactttctg aacgcttagc tcttgtggca gaagttggga
     4441 aagtcaaatc agaacacggc attcctgttt atgctccgga gcgagaaacc gcaatgattt
     4501 ccgcccgccg ctctgaagcg gaaaaacaag gtattccggc ggatctcatt gaagatgtgc
     4561 tacgccgtat aatgcgtgaa tcttatgcca atgaaaacaa acacggcttc aaaagagtta
     4621 attcggacat tcaaaaaatc gtgattgttg gtggtaaagg gaaattaggt gggctgtttg
     4681 gcaattattt aaccctatcc ggctataatg ttgaggcatt aggacgtgat gattgggaca
     4741 atgccgagca gattttagcc aatgcagatg tggttattgt gtgtgtgccg attgccaaca
     4801 ccctagcaac catcgagcgt ttacaatctt atttaaccga aaatatgatt ttggcggatt
     4861 taacctccgt caaacatcag ccgttgcaaa aaatgctgga agtgcataat ggagcggtag
     4921 tcggtttaca cccgatgttc ggaccggata ttgcaagtca ggcaaagcaa gtggtcgctt
     4981 gctgccacgg gcgtttccct gaacgttatc aatggttgct tgagcaaatc aaaatttggg
     5041 gggcaaaaat tgaagagatt gaggcagcag cacacgataa tgcaatgacc tacattcaag
     5101 ccctacgcca cttttccact tttgcattcg ggctacatct ttctcgccag ccggtgcaac
     5161 tctctcaatt attggcacta tcttcgccga tttaccgctt agagttggca atgattgggc
     5221 gtttatttgc ccaagacggc agcttatatg ccgatattat tgccgataaa ccggaaaatt
     5281 tagccaccat cgaaacctta aaagagagtt ttgaacaagg gctagatttc tttaaacgca
     5341 acgacaaagc cggttttatc aaggcttttg aggaagtgca tcactggttt ggcgattatt
     5401 ccgagcagtt cttgaaagag agtagagtat tgctccaaca ggctcacgat tcaagaaagt
     5461 aaaggtaggt tgaggttttg ctctgatagc actagcgtcc acgctagtgc tatttaatat
     5521 tctacaagcg gttaataaag agctgttttt tgcaaatttt tatgcaaaat tatccgcttg
     5581 ttttggcacg agcgtggacg ctcgcgccat cggaagaaac atttttgtaa aattgccccc
     5641 aaatctgacc gcttactgtg gctgaatttc tccatttttt accgtaaaca aacaatcatc
     5701 gcttctgtct tgccattgca tttgattgag ctgatcttga gtaatggcgg taacgaatac
     5761 ttgtgagcca ctttctctta aacgatgtgc taaaagctca cgtttggttg ggtcgagttc
     5821 ggaggcaaaa tcgtcaatta aaaataggca ttggcgttgt ttttgggcga ccaaatgttc
     5881 gccttgagcc agtcttaacg cacacatcaa tagctttaat tgcccacggg aaagcacatc
     5941 ttccaccggt aagccgttcg ctttaaaacg gaaatcggct ttttgagctc caatcatcgt
     6001 atagccaaga gctttatccc gctcaaaacc ttgtgccaga atctcggcat aatccgcccc
     6061 tttttcccag ccttgatgaa agctcaagcc gatttcgatt tccggcagga aaaattggca
     6121 ggttttctca atttcggggc gtaacgcttc ggcgtattcg gctcgcattt cgctcacggc
     6181 ataagtggtt ttcaccaatt cgatatccca cgctttgagt tcttgataag agcggacttg
     6241 ttgcaaagcc gaattacgtt gctttaataa acggcgaaga ttgttccaat gagcgtagaa
     6301 atcagggtgc tggtgaaata agccccagtc caaaaaagca cggcgaaagc tcggaccacc
     6361 gttaagtaag gttaagcctt cgggggtgat cacttgcatc ggcaataaat gggctaaatc
     6421 ggcaattttg ttgccgtctt caccgttgat tttaagtgtc gtctcgccac tgcgaaattt
     6481 ctgaatgccg accgaccaac tgtgctttgc ctcgtcaatt ttaccgtgta gtacgaaatc
     6541 gtctttatcg taatggataa tgcggttgct gatatggctt ttgaatgaac gcccgtgtcc
     6601 aagatagaaa attgattcca acaaactggt tttgccactt ccgttcgccc cgactaaaaa
     6661 attaaaatgc gggctaaatg ctaaatcggt atgttggata tttctaaaat ggttgattaa
     6721 taaacgggtt aaagccatag ttaaagtgcg aggaataaaa agagtagcca ttttagcaaa
     6781 tttgcaaaat tttgggaaaa attaaccgct tgcggattag aaagtggttc agtagaataa
     6841 gccgtttatt ttttattgaa aattaagagg aaaaattatg tttggaaaag gtggcttagg
     6901 cggcttaatg aaacaggctc agcaaatgca agagcgtatg caaaaaatgc aagaagagat
     6961 cgcccaatta gaagtaacag gcgagtctgg tgcgggtttg gtaaaagtca ctattaacgg
     7021 ggctcacaat tgccgccgaa ttgaaattga tccatcatta atggaagatg ataaagagat
     7081 ggtggaagat ttagttgccg ctgcctttaa cgatgcggtt cgccgtgcag aagaaatgca
     7141 aaaagagaaa atggcaagtg tgactgccgg tatgcaactg ccgccgggta tgaaattccc
     7201 attctaattt aagtatgcaa accagtcctt tacttgaaaa tttgatggaa gccttgcgtg
     7261 cattgcccgg tgtcggaccg aaatcggcac agcgaatggc ttaccatctg ttgcaacgta
     7321 atcgttccgg cggtgtggcg ttgtcaaaag ccttaaatga ggcaatgagc catatcggac
     7381 attgcaaatc gtgccgtact tttaccgaag aggaagagtg tacgatttgt aaaaatcctc
     7441 gccgtcagat gagcgggcaa ctttgtgtgg tagaaatgcc ggaagacatt caagcgattg
     7501 agcaaaccgg gcaattttca gggcgttatt ttgtgctgat ggggcatttg tcgccgattg
     7561 atggtatagg ccctcgtgaa atcggcttag atttattgca acaacgcttg gaacaggaat
     7621 ctttccacga agtgattttg gcgactaatc cgaccattga aggtgatgct accgcaaact
     7681 acattgctga aatgtgcaga atgtataacg tgaaagctac tcgtattgct cacggtgtgc
     7741 cggtcggtgg ttcattagaa atggttgatg gcacaacgct ttctcactct tttgcaggac
     7801 ggcgtgatat tgatatttaa agctatttct gtgctaactt aattctgata taattactca
     7861 actattttaa attattattt tgaggctaaa aatggaaact attgacaaaa tcaaacaaca
     7921 aatcagcgaa aatccgattc tactttatat gaaaggttca ccgaaattcc catcttgtgg
     7981 tttctccgct cgtgcggtgg aagcggtaat taactgccaa gtgcctttcg gttatgtgga
     8041 tattttaacc aatccggata ttcgtgcgga attgccaaaa tttgcaaact ggccgacttt
     8101 cccacaatta tgggtagaag gtgaattagt tggcggttgt gacatcgtat tagaaatgct
     8161 ccaaaaaggc gaacttcaaa ccttattaaa agaaaccgca gcaaaacacg cagcataatt
     8221 tcaaaccagt atgttaaggc tcttatgttt aagagcctta attttttctt gcgatttagg
     8281 cttaaacttc ttacctcaag agctattttg ttttagaatg tcaaaattat ttcgtaacat
     8341 aaaaataaaa ggacacaaca tggcattaaa agagttagat tgggcaaatt taggcttttc
     8401 ttatatcaaa accgattacc gtttcattgc acattggaaa gacggaaaat ggaatgaagg
     8461 cgaattaact caagacaata cactgcatat tcacgaaggc tcaactgccc tgcactatgg
     8521 gcaacaatgt tttgaggggt taaaagccta tcgctgtaaa gatggctcaa ttaacttatt
     8581 ccgcccggat caaaatgccg agcgtatgca acgcaccgca gatcgtttat taatgccacg
     8641 tgtgccaacc gagctgttta ttcgtgcctg taaaaaagtg gtaaaagcca atcaagattg
     8701 gttagggcct tacggttcgg gggcaaccct ttatttacgt ccgtttttaa tcggcgtggg
     8761 cgaaaatatt ggggtgaaag ctgcacctga atttattttt tccgtattct gctgtccggt
     8821 gggggcatat ttcaaaggtg gcttagcacc ctcaaacttc attacaaccg actatgaccg
     8881 tgcagcccca atgggaacag gtggtgtgaa agtaggcggt aactacgctg cgagtttatt
     8941 accgcacgaa ttggcggttg agcaaagttc aactacccgt aaatttgccg atgcgattta
     9001 tttagatccg aaaacccata ccaaaattga agaagtgggg gcggcgaact tctttgggat
     9061 taccaaagac aacaaattca tcaccccgat ttcagagtcg attttgccaa gtattaccaa
     9121 atactcattg ctacaccttg ctcaagagcg tttaggtatg gaagcgattg agggagatgt
     9181 gtatatcgac cagttagacc aatttgccga agccggtgct tgtggtacgg ctgcggtgat
     9241 tacgccggtt ggtggtattc aacataaaga caaattccac gtcttctatt cagaaaccga
     9301 agtggggcct gtcacccgcc gtttatacgc agaattaacc ggtattcaat ttggcgatgt
     9361 tgaagcaccg caaggctgga ttgtaaaagt agagtaattt tacacaagta acaagcggtc
     9421 gaattggcaa aactttttgc aaattcaacc gcttgtcttt atttagagaa tatgaaaaag
     9481 ttaatttttt taggtttatc agcacttgca ttagctggtt gttcctcaac aatttctaat
     9541 cctaatcaac tgattgggca atggcaatgc actattgagt acgatgattt taatattcgt
     9601 acggtggata atttacgatt ttccacaaat gggactatga ccaatcaagg tggtatatat
     9661 taccctattc aaaagtcgat ttttgagtat tcattacaac agaatgggcg ctggagtgtg
     9721 aaaaacgggg ctattgttta tcgtatcgtc tcagaatctg tacagcgtag ccatcgttca
     9781 caaatatggt ctgaattaca acgtgatgcg gagctacaac aatttgaaga gaatttattt
     9841 agctcacttt ctgatagtga taataataaa acagtggaac taatcatcac tgattttgat
     9901 gaaaaagaaa tggatatcaa gcaagaaata aaagggcata aagcttataa agggcaatgt
     9961 ataaaacagt agaggtaaaa atgattcaac gttttgaagt aggtagccgt ttttcggaaa
    10021 tggcaataca taacggtgtg gcttacttgg cagggcaagt gccgttagat gacaccgccg
    10081 atgcttacac ccaaacccag caagtgcttg cagaaattga taaatggctg gcaaaagcca
    10141 acagcgataa aagtaaaatt ctaatggcta cggtttattt aaaagagatg gcagattatg
    10201 cggaaatgaa ccgtgcgtgg gacgaatggg ttgccccaaa taacgctcca ccaagagcgg
    10261 cggtggaggc taaattagcc aatccaaatt ggaaagtcga aattgtggtt accgctgcgg
    10321 tataaatgaa aagcagaaag acgatctctg tttttcatta tgattcgtac attttttcga
    10381 tttgttgctt atagcgttct agaatgacct ttcgtcttaa ttttaatgta ggcgtaattt
    10441 cttccatttt ggtgctaaat gcttgcgaaa gtagtgtaaa ctttttgatc tgttcaaaac
    10501 ttgcaaggtc tttttgtaat tcgttaatgc gttgttcaaa cattctgaca acctcataat
    10561 gtttaatgag ttctaatctg tcttgatagc gaatattgag cttttttgca tactcttcga
    10621 ggctatcaaa gcaaggaact atcaacgcag aaacatattt tttggcatca gcaatcaccg
    10681 caatttgctc aataaactta tctttaccta tttttccttc aatgtattgt ggggcaatat
    10741 atttaccgtt tgaagttttc attaattctt taatacgatc ggtaatataa agattgcctt
    10801 ccgaatccag ttcaccggca tctcccgttt ttagaaaacc ttcagcagta aaggcttgag
    10861 cagtggcttc cggttttttg tagtaacctt tcataaccaa gcctccacgc actaaaattt
    10921 cattttcttc actaattttg acctcaacat caggcattaa ggtaccggtt gagttgatat
    10981 taaagccttt ttcctgccag catgaaatag tggcagtagt ttcggtcatt ccgtatccaa
    11041 gttttacatt gatgcctata ctttggaaaa atagcccgat agacggatct aatttagctc
    11101 ctccacacgg catcatacga atacgaccgc ctagtaaggc tcgtaatttt cctagcacca
    11161 atttatctgc cataatgtac tgcaagcggt ggaaaaaggc tgatttttta ccaaacagat
    11221 tgtcttcggc ggtacggatt gcccatttaa ataacgctcg gcgatgtatc ggtgcttgtt
    11281 ggactttatc ccacaccgca gtatagattt tttcatagaa ccttggtacg gcacacatta
    11341 atgtaggtcg taccgttgag agtgcttgac gtacctgttg aggatcttcc agatagcaat
    11401 ttactgcccc tctgtatagc acataagtga cccaagcacg ttcaaagata tgcgataaag
    11461 gtaaaaatga aagggatata tcattttcgt caatatgagg gagtgcttta ttatgtgcct
    11521 gaagttgatg agctaaatta gcataatcta acataacacc tttaggctca ccggttgttc
    11581 ccgaggtata aattaaagtg aataaatcct ctaagcattt atcatttaaa cgtttggtta
    11641 attcagcttc atcttgatta accttaatga aatcttgcca atgttgagcg atagggaaat
    11701 cacgcagatc tattgagtct ttcatcgcga caatttttat taatagcgaa cagttgttcg
    11761 ctatttctat ggcttgatca tattgttcct gatcgccaac aaagaggatt tttatatccc
    11821 cgtcattcag gatatattca agttgtttgg tggtactggt tgcataaatc ggcactgcta
    11881 ccgcacgaat ttgcattgca gcaatatcgg taattgtcca acgaggcata ttttgtgcaa
    11941 aaatagcgat tttctcttga acggcaagac cgtttgccag caaggcataa gaaagattat
    12001 tgacctgttt ttgtaattct aaccaactta tttctgacca tttttcatct tgcataaaac
    12061 gtaaagctgg gtggtggcta agagcctgaa cttgctggtg gaaacggtgg ataaagtgaa
    12121 aatctaaagt gctcataatc ttatttttac tttctatttc agtgaacagt tgtacgctaa
    12181 tataatagaa aatatgaaaa aaagaaacag ttgtactctg aaacaaaggg ttttggatag
    12241 gaagtaatca aggcaataga aatagataag tagggtatcg tttagtacaa atgcaatatt
    12301 tttcaaaaat gagaccgctt gtatatgcaa caagcggtct gttttgttaa aaattttgca
    12361 actaaattcg ttcaatatca aattgagcta aatcaatcac atcaggtgta ccgtaaaaag
    12421 cacagagggc tttacgcaag gtttcagctc gtttgggaag gtagccacgt tctgcataga
    12481 gcttgacttg gtcgtacact gcttgtttaa aggctgaatt atcgccggga ttgcccgaga
    12541 gattgtcggc actagcaaaa taacgaaaac cggcagcagt tgctaacacc cattcaatcg
    12601 cttgcggttt gatttccgca ctttcaaatt cacgttggcg ttcggcagag cgtccgtccg
    12661 gctcgtacca atagccaaaa tcttcaagtt tacgccgagc ttctccagca acgagccaat
    12721 gggcaatttc gtgcaaggca ctgctgtaat agccgtgagc aaataaaatc acattgtaag
    12781 ggcgttcgct cggcacacca tcttcgtcca taaattcggg caagtaaatc ggataattac
    12841 ccccccgctc taggcgagtg ttatattcat cagaaaagca ttgattaaaa atgcgaataa
    12901 tgtcttcaat tttatgattt tcttgattca tttcttttct ttataactta ttcagccaag
    12961 ctaaaaattg ctcggcatcc aaaaagccag taacacgaga ttggctcatt tcattgccgt
    13021 tttcatcaaa aaacagaatc gttggcaaac ctaatacatt aaagtgtttc atcagctcga
    13081 tattttcagc cgagtttttc gtcatatcga tttgcaacac taccatttca ttcagtttgt
    13141 tctgaacatt ttggtcacta aaggtgtatt tttcaaactc tttgcaggcg acacaccagt
    13201 cggcatagag atcgagcatc acttttttac ctttggaagc ggtcaatttt gtctctaaat
    13261 cggcaagtga ttgaattttt tctaacgcta agtgcggtgt agtttgctgt tgagtggttg
    13321 agccgttcca agctaaatct gcccaaggtt tagcggcaat ggctaaagcg agcaataata
    13381 agactttgat gatctttttc gtgattgaat tagatggaat cgcatccacc aaccagatca
    13441 aaaatgcaac cgcaagggcc gaccataaaa acggttcata gtggcttggc agcacacggc
    13501 ttaataagaa caccggtaag gcaagcataa caaaaccgaa tgcagctttc acttttaaca
    13561 gccattcgcc ggattttggc agaattttgt tgccgaataa ggtaatcaaa atcagcggta
    13621 agcccattcc cagtgcgagt agatagaggg ctaagccacc ggtgactaaa tcgccacttt
    13681 gtgccacata aagcaacgca ccggaaagtg gggcagaagt acaaggggaa gcaattaagc
    13741 ctgcgatcat ccccattaaa aacacgctac ccaacgcacc accttgctgt ttttggctca
    13801 ttgcattgag tttttgttgc cagctatttg gtagctggat ttcgtataag ccaaacattg
    13861 agcaggcaag cagaataaag acgatagata aaccaatcaa caccggtggg ctttgtaagg
    13921 cgatttggaa aggcaagccg atagccgcta caattaagcc aagtaaggtg taagtaactg
    13981 ccattccttg cacataggca atacttaaca gcaatgcttt agcggtagaa gggcgttgct
    14041 tatttccaat tacaatcgca gagagaagtg gtagcatcgg cagtacgcaa ggagtgaatg
    14101 ctaatccaac gcctaataca aaaaaccaaa aaatggaaaa acgattgtta gctaaatttt
    14161 cagccagttg atcttgctct gcttttgcca cattttcagg gggcgccata ttgtcggttt
    14221 ttgcaaaatt ttcgctcttt tcgaccgctt gtgcttccag ttgcgccagt tcttcaggtg
    14281 tagcagagag aatactgtct aatttaagct caacccgttc cggtggatag caaaagcctt
    14341 tggtacaccc ttgatattcc actacgattg aggcgttgtc tttaatttct ttaaacggta
    14401 cctttagccc taagtggttg cgatagattt ccacctgccc gaaaaattca tcgttatatt
    14461 gctcggcagc gggaaaagtg atgtcgccta tctcaatatt ctgtggcgtg acacggattt
    14521 cttttttata aagataataa ttgtctgcaa tatcccaatt taactgtaat gtttcatttt
    14581 cattttggat tgaaaaaaca aaggcttctt ccgattttag gaatttgggt tgagagaaaa
    14641 gatttgcgtg agatgagttt atcatcaata aactcaagac gagtgaaaaa aagtatctaa
    14701 tcatattgaa atcctatttt taattgtctc ggcgaaaatt atagcgtaat cagtattcat
    14761 attccaaata aattacgcca tttaac
//
LOCUS       DANLUA010000045        14296 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLUA010000045 DANLUA010000000
VERSION     DANLUA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(107..1066)
                     /locus_tag="PXB44_001002"
     CDS             complement(107..1066)
                     /locus_tag="PXB44_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5281500.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            complement(1263..1670)
                     /locus_tag="PXB44_001003"
     CDS             complement(1263..1670)
                     /locus_tag="PXB44_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL5281501.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            complement(1670..2314)
                     /gene="sspA"
                     /locus_tag="PXB44_001004"
     CDS             complement(1670..2314)
                     /gene="sspA"
                     /locus_tag="PXB44_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL5281502.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            2488..2985
                     /gene="purE"
                     /locus_tag="PXB44_001005"
     CDS             2488..2985
                     /gene="purE"
                     /locus_tag="PXB44_001005"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL5281503.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            3000..3965
                     /locus_tag="PXB44_001006"
     CDS             3000..3965
                     /locus_tag="PXB44_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL5281504.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            3984..5072
                     /gene="purK"
                     /locus_tag="PXB44_001007"
     CDS             3984..5072
                     /gene="purK"
                     /locus_tag="PXB44_001007"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL5281505.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(5293..5703)
                     /locus_tag="PXB44_001008"
     CDS             complement(5293..5703)
                     /locus_tag="PXB44_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281506.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(5706..7724)
                     /gene="uvrB"
                     /locus_tag="PXB44_001009"
     CDS             complement(5706..7724)
                     /gene="uvrB"
                     /locus_tag="PXB44_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL5281507.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(7963..8199)
                     /locus_tag="PXB44_001010"
     CDS             complement(7963..8199)
                     /locus_tag="PXB44_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5281508.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            8362..9207
                     /locus_tag="PXB44_001011"
     CDS             8362..9207
                     /locus_tag="PXB44_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL5281509.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            9218..10414
                     /gene="efeB"
                     /locus_tag="PXB44_001012"
     CDS             9218..10414
                     /gene="efeB"
                     /locus_tag="PXB44_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL5281510.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            10425..11044
                     /locus_tag="PXB44_001013"
                     /pseudo
     CDS             10425..11044
                     /locus_tag="PXB44_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(11080..11898)
                     /locus_tag="PXB44_001014"
     CDS             complement(11080..11898)
                     /locus_tag="PXB44_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL5281511.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(11910..12509)
                     /locus_tag="PXB44_001015"
     CDS             complement(11910..12509)
                     /locus_tag="PXB44_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL5281512.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(12506..14053)
                     /gene="sbcB"
                     /locus_tag="PXB44_001016"
     CDS             complement(12506..14053)
                     /gene="sbcB"
                     /locus_tag="PXB44_001016"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL5281513.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         4215 a         3106 c         2867 g         4108 t
ORIGIN      
        1 aacaataatt gcagcaatcc atcccataat attctcctta gctgagttga aatgaatttt
       61 tactctacaa gattatgaca aataaaacaa atttttattt tttacactaa aaagtttgtt
      121 tagcttcttt gattaatacc tctggtatag taatgcccaa ttcttttgcc gttttagtat
      181 taattgaaag ctctaatttg ctgacttctt ggattggaat tgtacctgct ttttcccctt
      241 ttaatacacg agcagcaact ttaccggttg ccacaccaat atcatattga ttaaccgcat
      301 aagctgccac tgcaccacgt tgtactgtat cacggtcagc agcaatcacc ggaatttttg
      361 cttctaaagc cgctttatgt aaggcttcat aagccgaaac aactgcatta tcttcagaaa
      421 tataaatagc ctgcactttt ccgtttaggc tgcgagcagc agtgccaata tcggcactgc
      481 gttgaacagc aaccggcact atagtaaagc cctgtttttg agcctcttgt tttaactctt
      541 ctaacacaat cgcagaatta acttcacctg cactatacac ataaccaaca gctttcaaat
      601 ccggtactaa tgtttgaatt aatttcactt gtggagccat cgctttgtgg tctgaaatcc
      661 ccgtcacgtt agtgccactt ggctcccacg attttactaa ttttgcagca accgggtctg
      721 tcacgcctga aaacacaatt gggatagaac gtgttgcagc aacaacaggt tgtgccgaag
      781 gtgtggtgat cgggataata atatctgctt tatccccaac aaatttacgc gcaatttgtg
      841 ctgctgttgc agtactaccc tgtgcagact gatagtcgat tttaatgttt ttaccatcaa
      901 caaacccttc tcgggctaat tcttcaatca cacctttacg aattgaatct aatgcagggt
      961 gttccacaat cgctgtaatt gctacatttt gaattttatc ttgagctaat gccgctacag
     1021 aaagtgaact taacaaagtc actaataagg ttttatggaa tttcattttc tctccttaag
     1081 atcattactg tactatcaca acagtacaaa cacaaaacaa agataagcaa tttttagtcg
     1141 gagtgcaagt ggaatttgat tatttccgtc acaaaacaaa caagcggtta aattttgact
     1201 gatatttgca aataatcaga aaaatttaac cgcttgtaat gtgttttgat gaaatagatc
     1261 tcttatttca ccaactttaa ggtagatgtt ttcttcgcct tgattttttt aggctgctct
     1321 actgctctaa ctcgagcttg ataagcctct tcagtgtagt aggcttcttc ttggaacata
     1381 ataccatcac cggtttcttg cgcataaatc gccaccattg ccccaaaagg aatataaata
     1441 tctcttaaca ttccctgaaa acgagcgcta aagctaatgt attcatcagc cacaatatac
     1501 tgcccgatgg aacgaggcga aatatttaaa ataatcttcc cttctttgac aaactctacc
     1561 ggcacatcta cgtctggaaa ttctgcattc actaataagt aaggcgtgtt atcgctatca
     1621 ataatccagt tatagtaggc attataaaga taaggtctta atggtttcat tagtctttat
     1681 cgtccattaa atgttttggc gcttcgccgc caacagactg gataaaacta tcacgttgga
     1741 atacacgggt catatacgca ttaattggtt ttgccgttgc accgctgaat ttaacgccta
     1801 agatttgcat acgccataat aacggtgcaa tatagcaatc aaccaagctg aaatcatcgc
     1861 tcataaaata aggtttagcg gcaaaaatag gcgctaatgc caatacatct tctttaagct
     1921 gtgctaacgc tttcttgcct tccgctgatt caggatcttt attcacaata tcaatttgcg
     1981 aataccaatc ttgttcaata cgatacatat ttaaacggca ctgaccacga gaaacagggt
     2041 aaaccggcat taatggtggg tgagggaaac gctcatctaa atattccata ataatacgag
     2101 ggttaaacag cactaattca cgatcaacca atgtaggaat attaccgtaa ggattcaatt
     2161 ctaataaatc ttcagaaata ctgcctagca taacgttctc tatttcataa ggcacccctt
     2221 tttctgctaa cacaatacgc acttggtggc tgtaaatatc atttttatcc gaaaaaagag
     2281 tcattaccga acgtttgttt atactactcg tcatttatat ctccgatgat taaatttata
     2341 aaaaataaaa tggttgtcga ttatatcata ttttaaccag cagataacag ccctaaaaat
     2401 tgcttttcac ttcttaaata gctacgcaaa cgtttgcttt gtatgctatg attacactgt
     2461 tttacattag tatcataagg atttaaaatg ccaaataaag ctgaaattgc gatcgtaatg
     2521 ggatctaaga gtgattgggc tacaatgtct gaagcaaccc aaatattaga ccagttcggt
     2581 ttgagctatc acgttgaagt cgtctcagca caccgcacac cggacaaact cttttctttt
     2641 gctgaaactg cagagactaa cggctataaa gtgattattg ccggagccgg cggtgcagct
     2701 cacttgcccg gaatgatcgc tgctaaaacc attgtgccgg tgttaggtgt gccggtaaaa
     2761 agctcaatgt taagtggtgt agatagcctc tattcgattg tgcaaatgcc gaaaggtatt
     2821 ccggtcggta cgctcgcaat cgggccggca ggtgcagcaa atgcaggctt attagccgct
     2881 caaattttag cagcctttaa ccctgatatt gcagagaaac tgcgtatttt ccgtcaaacc
     2941 caaactcaga tggtattaga taatcctgat cctcgtgttg aataactatt gtataaaaga
     3001 tgtctaaaaa attattaatc ctccacactg gtggtactat ttcaatgaaa gaaggggaag
     3061 acggtaaggt ctccccttcc aatgaaaatc ctctcttggc tgccttagaa cgtttaaacc
     3121 acccagcaaa attggcgcaa gaatcgttat ttaatattcc ctctccgcat atcaatatcg
     3181 agcattggca acagttaaaa atgcggattg aaaaggcagt gaatgaggag aatgtcgatg
     3241 gcgtagtaat cacccacggc acagatacct tggaagaaac ctcctatttt ctggatttag
     3301 ccctcagcgt aaatataccg gtagtaatta ccggagcaat gcgttccagt aacgaactgg
     3361 gtgctgatgg cttaatcaat ctacaaagtg cgattttagt ggcgttaaat gaagaaagcc
     3421 gagataaagg cgtgttagtg gtgatgaatg atgaaatcca caatgctaaa tttgtgacca
     3481 aaacccacac cactaatgtg gcgactttcc aaaccccaac ctttgggcct tgtggcttag
     3541 taacgaaaga atctgtcatt tttttccaaa aattgaccac ttacgaacgt tatccaattc
     3601 aacaagtaac taaagctaac gtgcaactac tgaaagcata cgccggaatg gatagttttc
     3661 tgctggaaca attagcgaaa agcaaatgcg atggtgttgt gattgaggca ttgggtgcag
     3721 gtaatttacc ccctagttgt ctggctggta ttgaggcttt agtgaatgcc aatattccaa
     3781 ttgtaattgt ttctcgggca ttcaatggta ttacccaaga tgtctatgat tatgtaggcg
     3841 gtggaaaaca gttaaagcaa aaaggcatta tttttaccaa aggcttaagt gggcaaaaag
     3901 ctcgcattaa acttatggtc ttactcagcc aaaccttaga caaaccacta tcagactatt
     3961 tctaacccat tgagaatacc acgatgcaaa aaagtgcaat ctacccacct gtgtatgtat
     4021 taggcaacgg ccagctcggg agaatgctac gctacgccgg agcaccactt gatattgaag
     4081 tcaaaccgct ggctttcgat gcccctgtgt ttgatattga gccaaacagc attattaccg
     4141 cagaaatcga acgttgggca gatacgcctt taacgcaatt actcggtaac cacccgaatt
     4201 ttgtgaacct gaatgttttt gggaagttag ccgatcgttt tacgcaaaaa tcactacttg
     4261 atgaactcca tcttgcgact tcaaaatggc aactgctaga aaatgccgaa cattggcaag
     4321 atatttttca gcatattggc gaaaaagtag tggttaaacg ccgaaccgga ggatatgacg
     4381 gacgtgggca atggattgta acgcaagact cgattcatca aattacacct gaattatttg
     4441 gcgaagtgat tgccgaaaaa tttatcccgt ttgaggggga aatttccatt gttggggcga
     4501 gattccgtga cggctcaacc cgtttctacc ctatttctca caatttacaa caaaacggca
     4561 ttctacgtta ctccgtctcc gatgtgaatt taccgaacca agccctctac cagcagcaag
     4621 ccgaacagat gctcgctacg gtaatgcaaa aattagagta tgtcggcgta atggcaatgg
     4681 aatgctttgt tgtgggcgac aagctactca tcaacgagct tgcccctcga gtgcataaca
     4741 gcggacactg gacacaactc ggctgtgcca ttagccaatt tgagttacat ttacgggcat
     4801 tattagatct acctacgcca gcactcgacc aaattgcccc aagcgtgatg gttaatttaa
     4861 taggcattga acataacaag cagtggctaa gcctcccatt tagccaacta cattggtatg
     4921 gcaaagaagt ccgagctggt agaaaagttg ggcatatcaa cctcactcac accgataaaa
     4981 acggcttaat tgaactactg gaacaactca gaccgagcct aacggaagat ttccattcgg
     5041 gattagattg ggcaatcaat aagctcaagt aagcggtcgg attttgtaac ttttttgcaa
     5101 atctgtttac ccgatggtgc gagcttcctc gctcgtacca aacaagcggt cgacttagtg
     5161 ctaaaatttg caaaataatt caacataatg gctacttccg gtcatcatct tgatctgaaa
     5221 acccctcagg gtggatatgg ctatcaaaaa gcattttctt cctccgagaa aacagatttc
     5281 ttcacaagaa tcttagagct gctctttatt tgtttcctta ggcaataact tcgagcaaat
     5341 gataatagag agcatagccc caaatcctgc acctgaaatc ttcagtgatt tatcataaac
     5401 tgccggctca aaataaataa atcccaaaat ccaaattaaa aagaacccca tataaatcca
     5461 aaacacttta tcttcatttt tctgggtata acctttataa acaataaccg cactacttac
     5521 catcatcgga ataataaaag tcaaaaacac attattgaac agggtaccaa atcgttcaaa
     5581 cggattactg tactcccgca taatatggtg ggcaatccag ataaccacca taaaaacaag
     5641 ttgagagaaa ataacaattt tggcaaataa gattaatttt gaaaataatg aacgagaaat
     5701 catatttaaa cctccaacaa atacgccttt aaatgctgca acttatccct taccgcagcc
     5761 gctttttcaa attccagatc tttcgcaaaa tcacgcattt gttgttccag cgttttaagc
     5821 tctttttcca gttctttacg ggatttcggc ttgtagtcgg ttgcagaatt ttcggctttt
     5881 ttgaccgctt gtttgccccg ttttggcttg tcggattgtc cgatgtcgag caattcaccc
     5941 acttttttgt tcaacgcttg tggcgtgatg ccgtgttctt cgttgtattt catctgcttt
     6001 tcacggcggc gttcggtctc agtaattgct ttttgcattg agttggtaat gcggtcgccg
     6061 tataaaatcg cttttccgtt gaggtttcgg gcggcacgtc cgatggtctg aattaacgag
     6121 cgttcggaac gtaaaaatcc ctctttatcc gcatcaagaa tggcaaccag cgacacttcg
     6181 ggcatatcta agccctcacg caataagttg atccccacca gcacatcaaa cattcccatt
     6241 cgtaaatcgt ggataatttc gacccgttcc accgtgtcaa tgtcgctatg tagataacga
     6301 actcgtacgc catgttcgtc caaataatcg gtgagatctt ccgccatttt tttggtgagt
     6361 gttgtcacca gcacccgttc gtccaccgcc actcgtttgt gaatttcaga cagtaaatcg
     6421 tccacctgcg tagcaacggg gcgaacttcg ataatcggat cgagcaagcc ggttggacga
     6481 actacttgat ccaccacatc gggatttttc tccaattcat acgcccccgg tgtggcagaa
     6541 acgtaaatcg tctgtggtga aagacgttca aactcttcaa atctcaacgg gcggttatcc
     6601 agtgcagacg gcagacggaa accgtattgc accaatgttt cttttctcgc tcggtcgcct
     6661 cgatacattc cgccaatttg tggcacagta acgtggcttt catcaatcac caaaataccg
     6721 tccgccggca tataatcaaa cagggtcgga ggggcttcgc cctcttttct gcccgagaga
     6781 tagcgtgagt agttttcaat gcccgaacag tagcccagct cgttcatcat ctcaatatca
     6841 aactgcgtac gttgggcgat ccgctgctct tccagcaatt tattctcttt gatgaaatag
     6901 gtgcgacggt cggcaagctc gaccttaatt tgctcgattg cctccaaaat ccgttcccga
     6961 ggggtcacat agtgagtttt cgggtaaatg gtatagcgag gcaccttgcc caagctatgc
     7021 cctgtaagcg gatcaaacag cgacagattt tcgatttcat cgtcaaacag ctctaccctt
     7081 aacgctactt catcgctttc ggcagggtaa atatcaatca catcgccccg cactcggaaa
     7141 gtcgaacgtt ggaacgcctg atcgttgcga gtatattgca attcggcaag gcgagagaga
     7201 atttcacgct gcccaatcat cgcccccact tgcaaatgca acatcatctg catataagcg
     7261 tccacatcac ccaagccgta aatggcagaa acggacgcca ctacaatcgt atctcggcgt
     7321 tccaaaaagg atttggttgc ggaaagtcgc atttgctcga tctgctcatt gatagaggca
     7381 tctttctcga taaaggtatc gcttgccggc acataggctt cgggctggta gtaatcatag
     7441 taagagacga aatactccac cgcattttcc ggaaagaacg ctttcatctc ggcataaagc
     7501 tgggcggcaa gggttttatt cggggcaagc accatcgccg ggcgattgag cttggcaatc
     7561 acattagcaa tggtaaaggt tttccccgag ccggttacgc caagtaaggt ttgatgtgcc
     7621 aagccgtcat ttaagccttc ggtcaatttg gcaatcgcac ttggttggtc gccggagggc
     7681 ttgaaaggag aatggaggat aaagggtttg ccttgcttgc tcatagaaaa tcctgtctta
     7741 aaaaatcggt ttattttagc acaagcaagc ggtcgttttt atcgggattt ttgcaaattt
     7801 attgtaactg cttatattga tccattcttg gatctaacac cctaacaata aagatacctt
     7861 ttgggattgg tttataataa atagaatgtg agcggtggta atgacagcgt aacaaagggc
     7921 gaataaagta acgctaaatc tcgctctgcc ctaacagaaa gtttatacat tacgttttgc
     7981 cttgctgata atagattgaa cggtttcctc agaaacaccg ctttctaagc cttcatcaat
     8041 taaggctctt agctgaatta gttgctgttc ttgtgcttcc aatattttta atgcggcact
     8101 gatgacctcg ttggtcgaac cataacgccc actttcaacc atttggttca caaattggct
     8161 taatggctca gctaaagtta cgctggttgt tctactcatt actattccta tcttattgat
     8221 tattgagcag attgtaatgg ctctaattta gataagcaag cggtctattt ttagcaataa
     8281 tttgcaaaac aaaatgtgat ttacatcaca aaatcagcat tattttttgc gaataattcg
     8341 caataaggat attatgtcgc aatattttta ttaaaaggaa atttctatgc gtttaaccaa
     8401 acttgctttt gcacttttgc actttctggc ttttttgtgg ctactcaagc ggcagcttta
     8461 gacttaacta aggaaaccga ggcttataaa caatttgtgg tagaacagat tgaccaactg
     8521 gttaccgata cggaaaaatt tgtcggctac ttaaacaaag gcgatgtgca aaaagcgaag
     8581 aaaatttacc cgcttgcaag aatgtatttt gaacgttctg aaccgattgc agaaagtttt
     8641 ggcgatttag accctcgtat tgatgcccgt ctggcagatt tagccgaaga aggtaaaacc
     8701 gagaaagatt ggtcaggttt ccataaaatc gagaaagtat tgtggcagaa aaacaccact
     8761 aaaggcacaa aagcaacggc agaacaatta ttaaaagatg taaaagagct gcgtgcgaaa
     8821 attcccactg cagaagttac ccctgagccg atgattaccg gtgcggtaga tttattaaac
     8881 gaagtttcta ctactaaagt aaccggtgaa gaagaaattt tctctaaaac cgacctttac
     8941 gattttaaag ccaatattga aggtgcagaa aaaatctatg agatttttaa aacgcaatta
     9001 gagcaaattg atgccaaact ctcaacagaa atcggctctc gttttgcggc tgtcaatgcg
     9061 ctgttagcaa aacacaataa atctaaaacc ggttatgatt atgtgcctta taacaaactg
     9121 tcaaaagcgg atattaaagc gttagcagag gctgtaaacc aattaggcga gccgttagct
     9181 caacttggcg tacttttaaa taaatagtgg agcaaaaatg atggcagaaa ccacttctcg
     9241 ccgtgatttt ttaaaacata ccgcattagt aggagcaggc ttattgtctg ctcctgcttt
     9301 cgctttggaa tcacgacaaa tatctgaaaa atctcaaaat caatacgact tttacggcaa
     9361 acatcaagct ggtattgtta gccctgcaca aaaacatatc tactttttag tgctggattt
     9421 agacaccact gacattgcca aagtaaaaac attgtttcaa aactggacgg aatatagccg
     9481 taacctcacg cttggcaaaa atgtgaaacc ttatagtgct aatgcttatg ttccaccggc
     9541 ggataccggt gaggcagata gcctttcggc acagaatctc actcttactt ttggcgtcag
     9601 tgccaatttc tttacaaagc tcggcattga aaaacataag ccgaaagaac tgaatgattt
     9661 accgcatttc cctcgtgatc aactacaagc agaatacacc ggtggcgata tttgcattca
     9721 agcctgtgca gacgatccgc aagtggcttt ccacgctgtg cgtaatttgg tgcgtgttgc
     9781 ccgtggtgaa atcaaaatga aatggtcgca aatggggttt aactcctttg ccaatcaaga
     9841 cacacctcgt aacttattcg gttttaaaga cggcactgcc aacgcagaaa gtttaaaaga
     9901 tcaggaaaat acggtatggg tgcaacaaga gggctggctc aaaggcggtt cttatttagc
     9961 cgttcgccgt gttaaaatgt tcttggaaac ttgggatcgc acccatctcc aatcccaaga
    10021 agaaaccttc ggtcgtcacc gtgattccgg agccggaatc gggcatcagc acgaatttga
    10081 taaattagat ttagccaaaa aagacgaaaa aggcaatgca gtaattccgg aagtttcaca
    10141 cgctcatctt gccaataaaa cggggctgaa aatcctacgc cgttcattct cctattcaag
    10201 cggtattgat gataaagggc aatttgattc agggttgttg tttgtctctt tccaacaaag
    10261 tccggagcaa tttatcaaaa tccagaacgc tctcggcaat gtggataaaa tgaatgaata
    10321 catcactcat atcggcagtg gtttatttgc ctgttttgcc ggtgtgaaag atgaaaatga
    10381 ctaccttggc aaagcgttgt ttgatttaat ttagggaaaa ggaaatgaaa ttaaaagcat
    10441 tagtgctaat tccgttactc ttttcgagta caggattgct tgccaaagtg aatgtcagcc
    10501 ccttatttgt tcagctttcc gatgcaatgg cagagagtaa aaaaggggaa tttgcaaaat
    10561 ccagccaaaa tttgaccgct ttacagcacg attttgagca gattagccag cacgattccc
    10621 tcgccggagt tgaagtacag accgctctga aaaaagcgat tgaaacaccc aatgctgaaa
    10681 acttagagag cctttctaaa aactctatgc gtttgaaaaa gaacaaaacc ctagcggtta
    10741 tgctgaaaaa catcaggatt ttgtggcaaa aatgacgccg ctttatgcag aacttgctca
    10801 aaccgtgcca actcaagaaa tcgccaaaat caaatgggcg gcttatcagt ttggtaagaa
    10861 ttgggtaaaa caggaaaaag cagtacggga aatcagtctt ccccattatg gcaaatttga
    10921 gcgtattctt ggtttaatgc gaattaatct gaacgctgaa aagccggata tggcaaaaat
    10981 cagtgaactg gtttccgaac taggtgtggt aatggcagac tttaagcaag ttaaagtgaa
    11041 atagtaaaaa atcggggcaa attatttgcc ccgataacac taaaataacg tccaaatcgg
    11101 tttacattct tggggtaatt ccaaactttt accgagctca atccagccag ttgggtagtg
    11161 gaaatcagaa ccgaccgagc catataaatc aaactctttt gcccaacggg caagtaattg
    11221 ccgttgatca ggtgtttgtc cgcagcctga tacttcaatg ccatcgcctc cggcttgttt
    11281 gaaatctgcg attaaacggc gaatccaacg agcagtcatt ttatagcgta gcgggtgagc
    11341 aatcgaaatc acaccgcctg aggtgtgggt aacattcacc gcgtcttcta aactactcca
    11401 tctcggtttc acataggcag gtttccccat tcccagataa cgcttaaagg cgtgttcaat
    11461 attgcggaca tagccttgtt catacaaaaa acgcccataa tgtgcccgag ttacttcccc
    11521 tgttgccaac gcttttgccc cctcataggc atttgcaatc ccgacttttg ctaatttttc
    11581 accaatttcg accgctctct cttcccttaa ttgggcttgg ttatccaaaa acttcaccag
    11641 ctcctcgttg ttttcatcaa tattcaacgc cgcaagatga atactttttt cttgccataa
    11701 aatcgaaatt tccaccccag agataagctc aagcggttga ttttgggcaa aatttttcgc
    11761 ttcttgaatg cccgaaatcg tatcgtgatc agttaaagct agcattgtta ccccttgctc
    11821 taccgcacgt tgtaccaatt cagtgggaga aagtacgcca tcagaggcgg tactgtgggc
    11881 gtgtaaatca tatttcattt tatttatcct taaattaagc cggcagtcct gaaaaattgg
    11941 cgataggctt cggttttgtt ggctaagctg agtggataag ccctcgacgt cgaaggcagg
    12001 cgatataaat aaagttctct tgtggcggaa taaggataga ccacccattc gttggttttc
    12061 ggagctttgt gtttttccgg caataaaccg agcaacgtat cggttgccaa accacctgtg
    12121 gtaaatagcc atttcagttt gggtaatttg agcaacagtt cgtccatatc gataggatca
    12181 acaattttta aatctttatc tgaagcgttt ccttttaaac gaatcgctgt ttttgccgaa
    12241 gaacagagcg caacgccctt ttctcgcaaa aacgcctcaa tccgaatcgg atcaaagcgt
    12301 ttttcttcac caactcgaaa atagttaggg tcagcataaa aaatattgcc cataattcgc
    12361 cacatatcat tttgaaaatt cgggtaatga aattccatac aacgcttttc cgatgtaggt
    12421 ggaaaagtgc cgaccattac caccgttgct tgttcaggta aaatagggtc aaacgggtgc
    12481 tgttctagcg gtaaattatc cattgtcatt taatcaactc ttttaacatc ttgagctttt
    12541 cggttttatc taggggctgt tccgctactc tgttaagctc gctcagcaat ttttcatctt
    12601 ttgcaacatt ttgacgataa atgaccgctt gttgttgcga aatctgctcg gcgtaagcgg
    12661 tcaaattttc taataatttt accttttccg ggttgtcgtg atgttgttgg aacaagtcgt
    12721 caatcgattg ggcgaactgc gctgcttttg cctcaatttg ctggttgcaa tgtttctgcc
    12781 atttgatctg ctcggctcga ctgagtgttt gtggatagtg ccttgctcgg taatggaata
    12841 gtaacgcctc tactcgaggg tcttcaaatt tcaagccgtg gttggctaaa tcttcaggcg
    12901 gtagcgtgcg taaaatcgcc atattatttt tatccgcagg cgagaaaaag ccgtcataca
    12961 aagtggtttc tacattggtc gaaggctcaa acgtgcgttc ttcattaaaa atttcaatca
    13021 ctttttcccg aatcagcgat ttctgtgctt ttaaagtttt caaattttcc aaacataaag
    13081 ctctgtctat gcctaaacgt tctgcatttt caggcagtaa ggtttttgcc ggtgctaaaa
    13141 tcgggcattt attaatatgc actaatttta acggcacagg taactcattt tcggctaatt
    13201 catctcgttt ggtgtataaa cgctgacgta aaacctctgc attttcagtc aataagccat
    13261 tcatatcgcc cgataaatca cacacaatta cggcattttg attagttgag tgccaagcca
    13321 gtggcacaac ccaagcggta tttcctcgat agttgcccag cataccggaa acgtgcacaa
    13381 gcggtgtcat ttcaccagtg tcaattaact tctctacttc ttttttactg cgtaaattaa
    13441 agaaaaaatc gaacaacttt ggctgttttt gcttaattaa ttttgccatt gcgatggtgg
    13501 catacacatc agacatcgca tcgtgtgcat tttcatgggc aatgccattg gctttggtga
    13561 ggttttccaa tttaaaactt ggcataccgg tttcatctgt tggccattca ataccatcag
    13621 gtcttaacgc ataacaggca cggactaaat ctaacaagtc ccaacgagaa tttccgtttt
    13681 tccagctata ttcgtaagga tcgtaaaaat tgcggaagaa ggtataacgg gtcatctcat
    13741 catcataacg aatattgtta taaccgatag tacaagtatt cgctcggcta aattcagcgt
    13801 gaattcgggc tgcaaattcc ggctcagata caccttcagc gttacattgt tgcggtgtaa
    13861 tcccggtaac catcaccgct tcggggctgg gtaaataatc cggcgtttgc ttacaaaaaa
    13921 acattaccgg ttcaccgata atattaaaat ccgcatcggt tcgaataccg gcaaattgtg
    13981 ccggacgatc taacgccgga ctcacgccga aactttcata atcgtaaaag aaaaaagaga
    14041 atttatcgct cattcgttca atttgttaga aaaaattggg ctgattgtag cacagtaagg
    14101 cagaaaggag ggaatacaag cggtcaattt ccgttagaaa tttgcaaatg ctaaaataaa
    14161 aaaagcccct aatcaaaaaa gcgaaaaggg gcggaggtct tacctaatga taatattatt
    14221 tttataaatc aattagttaa gatgtttttg tcgtaaaaat atcgagatta ttaaaaacac
    14281 ctacaataaa gcaata
//
LOCUS       DANLUA010000046        14166 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLUA010000046 DANLUA010000000
VERSION     DANLUA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            3..275
                     /locus_tag="PXB44_001017"
     CDS             3..275
                     /locus_tag="PXB44_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5281514.1"
                     /translation="MPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEP
                     ELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            259..501
                     /locus_tag="PXB44_001018"
     CDS             259..501
                     /locus_tag="PXB44_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL5281515.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            513..1181
                     /gene="rluA"
                     /locus_tag="PXB44_001019"
     CDS             513..1181
                     /gene="rluA"
                     /locus_tag="PXB44_001019"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL5281516.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(1159..1734)
                     /gene="ampD"
                     /locus_tag="PXB44_001020"
     CDS             complement(1159..1734)
                     /gene="ampD"
                     /locus_tag="PXB44_001020"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL5281517.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            1794..2636
                     /locus_tag="PXB44_001021"
     CDS             1794..2636
                     /locus_tag="PXB44_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL5281518.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            2759..3400
                     /gene="pyrE"
                     /locus_tag="PXB44_001022"
     CDS             2759..3400
                     /gene="pyrE"
                     /locus_tag="PXB44_001022"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL5281519.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            3574..4663
                     /locus_tag="PXB44_001023"
                     /pseudo
     CDS             3574..4663
                     /locus_tag="PXB44_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(4728..5918)
                     /gene="metC"
                     /locus_tag="PXB44_001024"
     CDS             complement(4728..5918)
                     /gene="metC"
                     /locus_tag="PXB44_001024"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL5281520.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(5977..6516)
                     /gene="dsbB"
                     /locus_tag="PXB44_001025"
     CDS             complement(5977..6516)
                     /gene="dsbB"
                     /locus_tag="PXB44_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL5281521.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(6579..8120)
                     /gene="nhaB"
                     /locus_tag="PXB44_001026"
     CDS             complement(6579..8120)
                     /gene="nhaB"
                     /locus_tag="PXB44_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL5281522.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WGVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
     gene            8277..9002
                     /gene="fadR"
                     /locus_tag="PXB44_001027"
     CDS             8277..9002
                     /gene="fadR"
                     /locus_tag="PXB44_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL5281523.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
     gene            9017..10087
                     /locus_tag="PXB44_001028"
     CDS             9017..10087
                     /locus_tag="PXB44_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL5281524.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(10213..12219)
                     /gene="tkt"
                     /locus_tag="PXB44_001029"
     CDS             complement(10213..12219)
                     /gene="tkt"
                     /locus_tag="PXB44_001029"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL5281525.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            12577..12789
                     /gene="rpmE"
                     /locus_tag="PXB44_001030"
     CDS             12577..12789
                     /gene="rpmE"
                     /locus_tag="PXB44_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL5281526.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            complement(12844..13899)
                     /locus_tag="PXB44_001031"
     CDS             complement(12844..13899)
                     /locus_tag="PXB44_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5281527.1"
                     /translation="MKSLVIKLFGTKKEKKTFKFSKIESVLLKPIGDAIGDAVVHIAH
                     LEQIKKALPNVKIAVLVTSRNKLIFSQANVVDMILEDKVSTYLSQRGHWDLYLDFKPT
                     FTTKSIILDSILKPKFIINFGKKQKSIYNLDTVKNYDFTTVFPKQVHFADYLNYAIFA
                     DFLEKNQVNYNLNIPNELSSFANTLWQEKKIRLLLNPQGSMREISTQELSELLSMIDK
                     RYVQHISCLLTNTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIAVDGGGV
                     HISCALGKKLLSFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPA
                     KWLNHQFAKLIEKYTAC"
     gene            13947..>14166
                     /locus_tag="PXB44_001032"
     CDS             13947..>14166
                     /locus_tag="PXB44_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5281528.1"
                     /translation="MNIKATLRKARLVIGKAILDSKNTDLILSEIPNKILFLRHDGKI
                     GDYVVSSFVFREIKKQSPQTQIGVVCSQK"
BASE COUNT         4366 a         2744 c         2974 g         4082 t
ORIGIN      
        1 ctgtgccgtt attgtttgaa tacgacccga acaaaagcca aattaattta gccaaatatg
       61 gtattgattt tgaacaggca aagttgcttt gggaagatga acgaaaagtt gtactggagg
      121 cttgcattga acccgaatta cgctatttcc tgattggcaa gatttatgat aaatattgga
      181 cagcgttttt tacaccaaga aatgatgtta ttcgtttaat ttcagttaga cgttcccgta
      241 aaaaagagat ctcatactat gagaaatacc attagtgccg aagaattaga acgtatcttc
      301 gatgaaggca aagaagatat tttacaatac tgcgatcttt ctaccgctca tcgcccgggg
      361 caaatgaaaa gggtgaatgt tgatttccca aattggatgg tagaagcgtt agataagcaa
      421 gccagacatt taggtgtgcc tcgccaagcc ttgattaaaa tgtggattgc ggaaaaatta
      481 ccggttcaat cacctaggta agcgagtggc aaatggcatt aattgaatat aaccctccat
      541 tagaaccatt tttagatgag gtttatcggg ataaccatat tttagtaatt aataagccta
      601 gtggtttatt atcggtgccg ggcaatcgtc ccgaatatta cgacagtgca atgactcgag
      661 tacagcaaaa atatggcttt accgaaccgg ctcatcgttt ggatatggca acaagcggta
      721 ttcttttgtt cgccctaagc aaagcggcgg aaaaagaact aaaacgccaa tttcgggaac
      781 gtgaaccgaa aaaacactat attgcgttac tttggggcaa attaggtgaa aaagttgggg
      841 ataagggcga aatgaatttt ccgctgattt gcgattggga aaatcgcccc cgccagaaaa
      901 tttgctatga acgaggcaaa aaagccatta cccattatga agtgttagct cattatccga
      961 ataatatagc gagagtaaaa ctcacgccta tcacggggcg ttcgcatcag ctacgagtac
     1021 atagccttgc gttaggtcac ccgattattg gcgataagtt ttatgctaat ccgctggcaa
     1081 aaagtttatc gccccgttta tgtttgcacg cggagtcact aacaattact cacccgatta
     1141 caagggagtt gatgacgttt actgctgagc cgacttttta gcctcgttca gtaggtgttt
     1201 gtaatatgcc caatcaaaat attgccccgg gtcgattttc cgttccgggg caatatggca
     1261 atgcccggtg atgcgttcat cggtaatttg tggatagctt tgctgaatgg ctttggtcaa
     1321 atttgccaaa gattggtatt gagcttgagt aaacggctga ttattactgc cttccagttc
     1381 aatgccaatc gaatattcat tgcatttctc ccgcccttca aaacaagaga cgccggcgtg
     1441 ccacgccata tcgttaaaat tcacatattg cgtgatttcc ccctccctat taatcaaaca
     1501 atgggcggaa acccgtagat ctttgatttc ttcaaaataa gggtgagcgg ttggttcgag
     1561 tttaccttgg aaaaacagat cgataaaatt tccaccaaat tcttccggcg gtaagctgat
     1621 gtagtgaata accaacaagg aaatatcctc ttgatcagga cgttggctaa aatgcggaga
     1681 cggcacttgt ttttgaccgg ttaaacagcc ctgttcgatt tttaaatttg gcatagtcta
     1741 aatagggtga atacgttaaa atatggctca ttttactaga aaaagagaag aatatgttaa
     1801 gttatcgcca cagtttccac gcaggcaacc acgctgatgt gctaaaacac attgtccagc
     1861 ttttgatctt gcgagcatta aaagaaaaag aaaaaggttt tttatatctt gatactcact
     1921 ccggtgtggg gcgttatagt ctgttaagtt ttgaatcgga aaaaacaggc gaatatttag
     1981 agggcgttgc ccgtttatgg gagcgtgatg atttaccgga agaagtggca ttctatattg
     2041 atgagctgaa aaaattaaac cgtggcggca agttaaagta ttacgcaggc tccccattat
     2101 tggcggtaca gcaactgcgt gagcaagacc gagccattct taccgaactt caccccaatg
     2161 attttccgct gttacgacaa gaatttgcca aagcgaaaaa tgtgcaaacc aagcgagaaa
     2221 atggctttca acaattaaaa tccgccctgc cgcctaaaga gcgtcgaggt tttgtgttga
     2281 ttgatccacc ttatgaatta aaagaagatt atgaattggt ggttaaagcg atagaagagg
     2341 gctacaaacg gtttgccact ggtgtgtatg ccatttggta tccggtggtg cttcgccaac
     2401 ataccaaacg tatagtgcga ggtttaatgg ataccggcat tcgcaaaatt ctacaaattg
     2461 agcttgccgt tcgtcctgat agcgaccaac gtggaatgac ggcaagtggt atgattgtga
     2521 tcaacccacc gtggcagctt gaaacccaga tgaaaaaaat cctgccatat ttaaccgagg
     2581 tgcttgtgcc ggaaggaaca ggttcttgga aggtggagtg gattacgcct gagtaaatta
     2641 aatacggata gcacaagcgt ggacgcttgc gccatcataa attctgattt gcaatatttt
     2701 ttagaaaaat aaccgcttgt cttatcaaga agtgggggaa atataaaagg aaaaaacgat
     2761 gcaacaatac aaacacgact ttattgaatt tgccttaagc cgcaatgtgc tgaaatttgg
     2821 cgaatttacc ttaaaatcag gcagaatcag cccctatttt ttcaatgctg ggctttttaa
     2881 caccgggcgc gatttagcca aattaggcga atattacgcc aaagccattc aagccagcgg
     2941 cattgagtat gatgtgttat tcgggccggc ttataaaggc attccgattg caaccacggt
     3001 tgccgtggca ctagccaacc tgttcgatgt ggataaacct tgttgcttta accgcaaaga
     3061 ggcaaaagat cacggtgaag gcggtaattt aatcggttct cccttaacgg gcaagatttt
     3121 gctggtggac gatgtgatta ccgcaggcac ggcaattcgt gagtcgatgg aaattatcaa
     3181 tgccaataat gcccagcttg ccggtgtgct gattgcgtta aaccgtaaag agaaaggcaa
     3241 gggcgagttg tctgcgattc aagaagtcga acgtgattac ggttgccaag ttttctcgat
     3301 tgtggatttt gatgatttgg tagcatttat tgaaacgtcg ccacaatacg ctccttattt
     3361 gccgaaaatg cgtgaatatc gtgaaaaata cggtgtttaa cttttgcaaa aaaacagcaa
     3421 aaactcaccg cttgtcactc tctaatctca ccattatgaa tcactttcat cttttggtga
     3481 gattttttca attaaaaagc cgataaattg attgtgagct ttagctaatt ggcttaggat
     3541 aaactcctgt aatacaggag ttggagaact aaaatggcac atcaaaaatt accttttcac
     3601 gcagacaccg ttggctcata tttgcgttcc gatgcttgga aaaaagctca cgcagactat
     3661 aaagcaggca acatttccct cgaacaacgt gatgaaattg tagaagcgga agtgaaaaaa
     3721 ttagtacaag catcaattag atgcgggcat tcaagttgtt acagacggcg aatatcaccg
     3781 ctcttggtgg catattgatt tcttggaaaa tctgaacggc attgaaggct atgtgccgga
     3841 aaaagcctat gcgtttaaag gggttgaggt tcgcccttat aatacacgtt gttgcggtaa
     3901 agtgtcttgg aatccgaatc acccttttat tgagcatttc aaaaaattca atgcgattgt
     3961 aggtggtcgt gcggtggcga aatttaccat tccaagtcca aaccaattaa tgtacccgat
     4021 tcagtgggat cacggcattt acccaaaccg tgaggaattt gccaaagatg tgcaacaggc
     4081 ttataaagag gcgattaaag cgttctatga tgccggctgt cgttatttgc aaattgatga
     4141 tgtctattgg gggtcgttgt gtaataacgt tggtcaaccg acatttgaag ccgataaagc
     4201 tcaagcggtg gcgaatattc aagccattct tgccgataag ccggaagatt taaccattac
     4261 cacccacgtt tgccgaggca actataaatc ttcttattta ttgacagggg cttatgatcc
     4321 gattgccgac ggcttatttg ctcaaaccaa ctatgatggg tatttcttag aatatgatga
     4381 cgagcgttca ggcggttttg agccgttaaa acattttgcc aacaacaaag ggcgtgtggt
     4441 attgggctta attagttcaa aattccctga attggaagat aaggaagcgg ttaaagcccg
     4501 tattcaagaa gcggcaaaat atgtgccgtt agagcagctt tgcctaagcc cgcaatgtgg
     4561 ttttgcctca accgaagagg gcaatattat gtcggaagca cagcaatggg cgaaagttcg
     4621 tcttgtggag gaaatcgccc aagaagtttg gggcgaagat taattagagt gaaaaatgcc
     4681 cgtaacaagc gggcattttt tgtgaatttt ttgcaaattc aagccattta gcgaatgcgg
     4741 ttaaaacctt gttctaaatc ggcaatcaaa tcttccaccg cttccaagcc aacgtgaatg
     4801 cgaattaatg ttcccgtcag tttgcgttca atcgccgggc ggattttggc aatttcttcc
     4861 ggctgattgg caagaatcag cgattcaaac ccaccccaag aatacgccat tgaaaacagt
     4921 tcaaaatgct ctaggaagtt ggctaatttg tcattcgaga gcttttcgca aagttcaaat
     4981 gaaaatagac cgcttgaacc taaaaaatcc cgcttaaaaa attcgtgccc cgggcagctt
     5041 tccaacgccg ggtgataaac tgctttcact tcagggcgtt gagccagcca gttagctacc
     5101 gcaatgctac tttgttcgtg ctgttttaag cgaatgccta aggtgcgtag cccccgagcg
     5161 gtgatgtaag cggaatcagc atccaccatt tgccccatta aataagagcc ttcacgcagt
     5221 ttatcccaac aacgggcgtt agaaaccgca gtaccgatca tcacatcaga atgcccgact
     5281 aaatatttag tgccggcttg aatggaaata tcaatgccat attctaaagc agggaacagc
     5341 acaccggcag accaagtgtt gtcgatcata atcacgattt ctgggttgat ttcacgagca
     5401 acacgcacga gagtcggaat atcaggcact tccattgtta gcgagctcgg cgattctaaa
     5461 aaaagcactt tggtgttcgg ttgcacaagg gctttcaagc cttcaccaat catcgggtca
     5521 taataagtag ttgaaacacc catacgtttt aacagcgcat tacagaattc ttgtgtcggt
     5581 tcgtaagctg cgcctgtcat taaaacgtgg ttgccttgtt ccacaaaagc taaaatcgca
     5641 ttggtgactg ccgccgcacc gcaagggtaa aggtagcaac ctgctccacc ttctaattca
     5701 cacatcgctt cttgtaatga aaaatgagtg agagtgccac gtctgccata aaataatgct
     5761 cctttgtagc ggttttgtgt agcgtgtttt ttctcttcta aggtttcaaa cactaaagat
     5821 gaagcacgct gaatcaccgg gttaactgct ccttgtgtgt tacgtttttt acgccctgta
     5881 tgaacaagtt tggtggaaag atttttatag ttagacataa cttttcctaa agtaaaatgc
     5941 ataggggcaa atcaacattt gcccctttat taataattaa cgaaacataa agttatttct
     6001 ttgacggcta cgtttaaatt ggctgattag cacaatgacc gcaacaatta agtaaaccgc
     6061 aaaagtaaag attagccatt gtgccattga aaagccgaac atttcccatt ggctttggct
     6121 acaattaacc ggaccggggg caaacacttg tggtagccat tggtcaagcg gcaaggtttg
     6181 cgggaaatta actttaaatt cgcattgatt ccatggtgat ggattgacct gataatcgtg
     6241 gtgtttgatt gaaagtacta gccctttaaa tgcgctacca agccaaatta gaattgccaa
     6301 gtagcgaata atcgcatatc tattaccaag aaatcctaaa ataccggcaa ataaaatacc
     6361 aaataaagca actcgctcat aaatacacat tacacaaggt actaatccca tcccatgttg
     6421 gaaataaagc gctgtgcctt caagtataag acagcttaac attagtaaaa accatgcact
     6481 gcgagattgt gatagatttt taaaaaaata gagcatagtt attcctcatt ttgtgaactt
     6541 aaggcattct accttatttg gtatgagtta tctataaact aacgtgtaat aaaccatccc
     6601 caatttatca ttacttcagt catagaaggt agtaagaatt ctaaccctaa gaaaccgaca
     6661 atagatagaa caatggtata aggtaatgcc atatagacca ttctgccgta agacagttta
     6721 attaacggcg caaaggagga cgttaataag aataagaaag cagcttgtcc gtttggggta
     6781 gcaacagaag gaagatttgt gccagtatta attgcaactg caatcaggtc gaactgctca
     6841 cgatctataa tgccggcatc tagtgcggtt ttagcttcat taatgtaaac agtaccgacg
     6901 aagacgttat ctgaaatcat cgagagcaaa ccattgaaaa tatagaataa tgaaagctgt
     6961 gaatgtggtt cggcagataa cacaaaggca atgatcggct caaataattt taaatcaata
     7021 atcacggcaa caaccgagaa aaacacaata agcaatgcag taaaaggcat agattcttgg
     7081 aatgctttcc ctaaagtgtg ttcatcagta atgccacaaa atgcagtgca aataatgatg
     7141 atggttaagc caataatccc tacatcggca agatgtagcg ctaagccaat aattaaccaa
     7201 attccagcaa tggcttgcac cagcatcttt aagcgatctt gtttcgtcat tttttgttct
     7261 tttagttggc tatagcgagc gagtacgccc caaactttgc taggaagccg gtctccataa
     7321 ccaaataatt tatatttttc tacaagaaag caggtcgcaa tcccacaaat taacacgggt
     7381 aagctaactg gtaatgctct aagtaagaat tcgataaatc cccattccgc ttgggaggca
     7441 ataattaagt tttgtggttc tcccaccatg gtcattacgc cacctaaagc tgagccaata
     7501 ccggcgtgca tcaataaact tcttaaaaaa ccacgaaatt gctctaaaat ttctttatta
     7561 gagatgattt tttcatcatt ggtaatatca gtagaatctt caaagttatt acctgatgcg
     7621 actttatgat atacgccgta gaaacctgtt ccaacgctaa tgattacagc aataaccgtt
     7681 aaagcatcaa gaaaggcgga taaaaaagca gcgcttaagc agaaagaaag ggaaagaatt
     7741 tttttcgaat gaatggcgac aagcagtttg gtgaaaatat aaagcaataa ttgcttcata
     7801 aagtaaatac cggcaaccat aaacattaaa agaagaataa catcaaagtt tgccataatt
     7861 tcttctttaa tgtggtgagg atttgtcata ccgattacaa ctgcttcaat cgctaataaa
     7921 ccacctggct gtaatggata acattttaat gccatagaga gagtaaaaat aaattcagcg
     7981 actagaaacc agcctgcaac aaaaggattg ataaagaaat aaatgagagg attgataatt
     8041 aaaaatgcca aaatgcacaa tttataccac tcggggcttt ttcctaagaa acttttgaac
     8101 agagcatcaa tgctactcat ataaaggacc tcaaaatttg taaacagata ttacaattgt
     8161 aatatagttt agattaaagg ataatagtat tactgaactt tttagtcgca ataattgatt
     8221 agaaataaag aatagttaga ttttagataa gcatattttt tgcttggggg gcattaatgg
     8281 gtcacagtga tattttaaaa gcgcaaagcc cggcagcgtt agcggaagaa tatattgtaa
     8341 agagtatttg gaataatcat ttccctgccg gctctgatct tcctgccgag cgagagttag
     8401 cagagcgcat tggggtaaca agaacaactt tacgtgaagt actacaacgt ctagcgcgag
     8461 aaggttggtt aagtattcag cacggcaagc caacaagagt gaatgatatt tgggaaactt
     8521 cggggcctaa tattattggc actattatta agctagataa aagctatctt ccagtggtga
     8581 ttgccaatgt ggtgtcatta cgtactcgta tggcggaggc ttatattcca gaagcgattt
     8641 accttgctcc tgaagaaagt gctaaactct ttgatggact agatgaatta gaaaatacag
     8701 cagctgctta tgctgaattt gattataagt tattcagaca atttactttt ttagccaata
     8761 agcctgtgta tgcgttgatc ctaaatagct ttaaatcaat gtatcatcaa gtcgcaaaaa
     8821 ttttctttgc tgatgaaaat aatcgtcaat taacgttaag cttttaccgt tcattattat
     8881 gtacttgtct tgcaaaagat agtgaaaaag cagccgcttg tatggctaaa aatcgccaat
     8941 ctagtggtga aatttggcaa aaattatccg ctaatattcc tgaaagcttt ggtcagcttt
     9001 aacagaaggt agaattttga acgaaattat ttttagctta gaaccgcaag ataatgctcg
     9061 tctgcaatcg ctttgtggtg catttgatga gcatttagcc ttaattgaaa aaagttttaa
     9121 tctcattatt gctcgaacag gcttcaactt tacgattcaa tcggaagatg acaatcacta
     9181 ttctgctacc ttggttcaaa atagtgtgaa gctgattaag caactgtata atgaaactgc
     9241 cccgattaaa ggcaaaatta aggaattgga cttagaagat atacatttgg cgattcagga
     9301 aagccgaatg ctactacaaa accaatggca agccggctcg caaggcgaaa tagcgattaa
     9361 aaccaagcga ggggtgatta aacctcgtag cgaacatcaa caagcctatt tacgaaatat
     9421 attaagccac gacatcagtt ttggtatcgg gcctgctggc acaggcaaaa cctttttagc
     9481 ggtagcggca gcggtggaat cgcttgaacg ccaagaaatc cgccgtattt tacttacccg
     9541 tccggcggtg gaagccggtg aaaagctcgg ttttttaccc ggcgatttag ggcaaaaaat
     9601 tgagccttac ttacgcccgt tatatgatgc cctgtttgaa atgcttggct ttgaaaaagc
     9661 tcaaaagttg atggaacgtg gtgtgattga aattgcccca cttgcttata tgcgagggcg
     9721 tactttaaac gatgcgttca tcattttaga tgaaagccaa aacacgacta ccgagcaaat
     9781 gaaaatgttc ttaacccgta ttggctttaa ttccaaagcg gtgattaccg gcgatattac
     9841 ccaagtggat ttgccccgta gccaaaaatc aggcttaaaa cacgcaatgg aagtgctaaa
     9901 agaagtgccg gaactcagct ttaatttctt tgacagcaaa gatattgtcc gccaccctgt
     9961 ggttgccaaa attgtgcaag cctatgatgc gtgggaagcg gttgatgaag agcgtaaaga
    10021 acagcgaaaa ctggaaaaat tagctttaga gcaagaaaaa attcttgctc aagctgaaat
    10081 gttatagctt acaagcggtc atatttcgct aattttttgc aaaaaatcgc tgaaatttca
    10141 ccgcttatca taacagttgt tcaacaaaaa agcgggaagt taattcccgc ttttctttat
    10201 cttattgttt cattataaga tttcttttgc tttagcaacc acgttttcaa cggtaaagcc
    10261 gaagagtttg aataattgat ctgctggtgc ggattcacca aagctattca tacctacaat
    10321 tcgaccatca aagccgacat atttatacca gaagtcagct aattgagctt caatcgccac
    10381 acgtttagtg actgaacgtg gtaataccga ttcacgatag gcttcatctt gtttgtcaaa
    10441 cacgttggtg cttggcattg aaactacacg cactttcacg ccttctgcat ctaacacatc
    10501 agcggctttc attgctaaat ctacttcaga accggttgca attaagatta aatccgggca
    10561 actgcctttt tcgcaacatt cacgcaagat atagccacca cgagagatgt tcgctaattg
    10621 ctccgcagta cgttgttgtt gagccagatt ttggcgggta aagattagtg ctgtcgggcc
    10681 gtctttacgt tctaccgccg ctttccaagc aacagcagat tctacttggt ctgccggacg
    10741 ccaggtttct aagtttggaa tttggcgtaa tgaagcagtt tgttctaccg gttggtgggt
    10801 cgggccgtct tcacctaaac cgatggagtc gtgtgtataa acaaataagg cacgttgctt
    10861 cattaaggct gccatacgca ccgcattgtg tgcatattcg tagaacatta agaaggttgc
    10921 accgtaagga atgaaaccac cgtgtagggc aatcccgttc ataatcgcag acataccgaa
    10981 ttcacgcacg ccgtagttga tgtagttacc atctacattg tgttccgcac gaatcggttt
    11041 tgagccagac cataaggtta agttagagct tgctaagtca gctgagccgc cgaggaattc
    11101 aggtaagatt ggcgcgtaag cttcgattgc attttgtgat gctttacggc ttgcaatggt
    11161 tgccggattt gcttgtaatt tttcaataaa agctttgctt tcagcttccc agttagccgg
    11221 taattcgcct gcgtaacggc gtttaaattc agcggctagt tcagggtatg ccgcttcata
    11281 agcagcaaat ttagcgttcc aatctttttc tgctaatgta ccttttgctt ttgcatccca
    11341 ctctgtataa atttcagctg gaatcacaaa tggggcatgt tcccaaccta aaaattcacg
    11401 agcggcttgg atttcagcat cacccaatgg cgcaccgtgg caatcgtgag agtttgattt
    11461 atttggtgaa ccatagccga taatggtttt acaaataatt aaagttggac gctcggtttc
    11521 ggcttgtgca ttttcaatcg caaatttgat ttgttctggg ttatggccat cgacattacg
    11581 gataacttgc cagccataag actcaaagcg ttgtgcggta tcatcagtga accaaccatc
    11641 aacgtggccg tcgatagaga tattgttatc atcgtagaaa gcaatcagtt tgcctaagcc
    11701 taatgtaccg gcgagagagc aagcttcgtg tgagatacct tccattaaac agccatcgcc
    11761 taagaaagcg taagtgtagt gatcgacaat tttatgacct tcacggttaa attgtgctgc
    11821 aagggttttt tccgcaatcg ctataccgac cgcattggta ataccttgac ctaacgggcc
    11881 tgtggtggtt tcaacacccg gtgcataacc atattcaggg tgaccaggtg ttttagaatg
    11941 taactgacgg aactgtttta aatcttcaat agagagatca tagccggtta aatgaagtaa
    12001 gctatagatt aacattgaac cgtgtccgtt agacagaaca aaacggtcac gatctgccca
    12061 tttaggatta gttggattgt gttttaaaaa atcacgccat aaaacttctg caatatcagc
    12121 catacccatt ggtgcgcctg ggtggcctga gttagctttt tgtacagcat ccatacttaa
    12181 aaaacgaatt gcattcgcta gcactttacg ttctgtcatt tgtgttactc ctaatcgaga
    12241 atgttatata aaatggaaag gatatcgaat tggattctac actattttgc tagaaaaact
    12301 caaaaatgga aatgagaaat gtgatctgta tcacaaaaaa tgattaaatt gtgatttgtt
    12361 ttgattggat tttaatagac ggttttttcc taatacaata ttttcttgat aaagcgaatg
    12421 tttagtagtt gatctaggca acaaatttat ctaaaatacg cactcttttt ggcgttgagc
    12481 atttgctcaa catttttatt aattacatat ggtacctatc agcggtagag attctaacaa
    12541 gataggcggc gatatggctt cttatgaggt tccccaatga aacaaggtat tcacccagaa
    12601 tataaagaaa ttacggcaac ttgctcttgc ggtaatgtag ttaaaactcg ctcaacagtg
    12661 ggtaaagatt taaacttaga cgtatgtggc aactgccacc cgttctatac tggtaaacaa
    12721 cgtgttgttg acaccggtgg tcgtgttgaa cgctttaaca aacgtttcag catcccaagc
    12781 tcaaaataat ttgattattg caaaccccgc taatgcgggg ttttgttttt cttatatatc
    12841 gaattaacaa gcggtgtatt tttctatcaa ttttgcaaat tggtgattta accattttgc
    12901 aggagctacc agagaaaaac ctcgagtatc attattatcc tcggtccatt tatctgcgat
    12961 caccataaaa gtttcaacat ctttcttagg ttttggatac cagcgtgcaa tattagctgg
    13021 atgattggca taaaatgaga gcaatttctt gcctaatgca caagaaatat ggacaccacc
    13081 tccatcaact gcaattacaa tatcagcaga gtttactaat gcacaataac ctaaaatatc
    13141 actattaggt gctaggctta tttcaaatgg ggctttaatt ttcgacaaat attcggcact
    13201 attggttgta ttggtcagca aacaactaat atgttgaaca tatcttttat caatcataga
    13261 taatagctct gataattctt gagtagaaat ttcacgcata ctaccttgag gattcaatag
    13321 taatcgaatt ttcttctctt gccatagcgt atttgcaaaa ctgcttagtt cattaggaat
    13381 gtttaaatta taattgacct gatttttttc taaaaaatca gcaaaaatag catagttgag
    13441 atagtcagca aaatgaacct gctttggaaa gacagtggtg aaatcgtaat tttttactgt
    13501 atctaaattg taaatacttt tttgtttttt accgaagtta ataataaatt taggctttaa
    13561 aatactatct aaaataatac tttttgtggt aaatgtcggt ttgaaatcta aataaagatc
    13621 ccaatgtccc cgctgcgaga gataagtgga tactttatct tctaagatca tatctaccac
    13681 gtttgcttga gagaaaatca atttgttacg agatgtaact aaaaccgcta tttttacatt
    13741 tgggagagct tttttaattt gctccaaatg agctatatgc acaacagcat cgccaatagc
    13801 atctccgata ggtttgagta atacggattc tattttgcta aatttgaagg tttttttctc
    13861 tttttttgta ccaaaaagtt ttataactaa ggatttcatc tctataatag gttgattctt
    13921 ttaatgattg gttatagtaa gtaaaaatga atattaaagc aacgttaaga aaagcaagat
    13981 tggtgatagg taaagccatt ttagatagta aaaatactga tctgatatta tcggaaattc
    14041 ccaataaaat tctatttctt cgtcacgatg gtaaaattgg tgattatgtc gttagttcct
    14101 ttgtcttccg tgaaataaaa aaacagtctc cccaaacgca aataggcgtg gtttgctcac
    14161 agaaaa
//
LOCUS       DANLUA010000047        13909 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLUA010000047 DANLUA010000000
VERSION     DANLUA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(3..722)
                     /locus_tag="PXB44_001033"
     CDS             complement(3..722)
                     /locus_tag="PXB44_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL5281529.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            complement(780..1517)
                     /gene="tsaA"
                     /locus_tag="PXB44_001034"
     CDS             complement(780..1517)
                     /gene="tsaA"
                     /locus_tag="PXB44_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL5281530.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            1697..2605
                     /locus_tag="PXB44_001035"
     CDS             1697..2605
                     /locus_tag="PXB44_001035"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL5281531.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            complement(2681..3667)
                     /locus_tag="PXB44_001036"
     CDS             complement(2681..3667)
                     /locus_tag="PXB44_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL5281532.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(3761..5632)
                     /gene="rpoD"
                     /locus_tag="PXB44_001037"
     CDS             complement(3761..5632)
                     /gene="rpoD"
                     /locus_tag="PXB44_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL5281533.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            complement(5891..7639)
                     /gene="dnaG"
                     /locus_tag="PXB44_001038"
     CDS             complement(5891..7639)
                     /gene="dnaG"
                     /locus_tag="PXB44_001038"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5281534.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(7793..8008)
                     /gene="rpsU"
                     /locus_tag="PXB44_001039"
     CDS             complement(7793..8008)
                     /gene="rpsU"
                     /locus_tag="PXB44_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL5281535.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(8156..8752)
                     /locus_tag="PXB44_001040"
     CDS             complement(8156..8752)
                     /locus_tag="PXB44_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL5281536.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            8894..10150
                     /locus_tag="PXB44_001041"
     CDS             8894..10150
                     /locus_tag="PXB44_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5281537.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRIQSGNI
                     AILLSLFSIAIAFYWLSKKRIKTGIAYLVFAMFGLFASILTGARGGWLAFIPICFFIL
                     FLNLRQLNKKAIFLILLALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSL
                     GARFDMWENALIGIREKPLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAF
                     VKRGVVGFMALMAILIIPFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLA
                     HNSGSLFYFFVLILLYHLVKQNEEII"
     gene            complement(10252..11259)
                     /gene="rfbB"
                     /locus_tag="PXB44_001042"
     CDS             complement(10252..11259)
                     /gene="rfbB"
                     /locus_tag="PXB44_001042"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL5281538.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
     gene            complement(11256..11837)
                     /locus_tag="PXB44_001043"
     CDS             complement(11256..11837)
                     /locus_tag="PXB44_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL5281539.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKNWYLRDKDEYVGIHIYFKNSSNFYYPW
                     ELQIWDKKDAEKNIQSHIKYKRNFVNQISYSPL"
     gene            complement(11858..13129)
                     /locus_tag="PXB44_001044"
     CDS             complement(11858..13129)
                     /locus_tag="PXB44_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL5281540.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEH
                     DFYYIKHFSLWLDILIIFKTLKTMLTGFGAR"
     gene            complement(13133..13909)
                     /locus_tag="PXB44_001045"
                     /pseudo
     CDS             complement(13133..13909)
                     /locus_tag="PXB44_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4034 a         2975 c         2482 g         4418 t
ORIGIN      
        1 ttctattcaa attccacact tcctgaagcg gtagctgaaa ttgtggtttt acctgcttct
       61 aacgggattt catccataga tttgctttgt gccattgatg aaactgcata cattgctctg
      121 tgttcataat atggtctacc accatcattt ggagtttgaa tattgatatt aactagctta
      181 tatgttttcg cccctaaggc tttttgaatc acatcggctt tatgctgaaa tttgctgata
      241 atttccagcg tcatttcatc ttcaagggac gccattttct ctggcgatac actaaaatca
      301 atgtaacgaa tcgccacatc tttacctaaa ttttccaaca ccgctgccat tgcatcaaaa
      361 tctttgcttt tcaactgaat gctaccagag gtatcccaac catcaatatt ccctttgttg
      421 ttataattaa cgtgattagt cacaccttca gcttgtactt caatcgatgg atgttttttt
      481 gcctcagcca atacacgatt caggttttca gatacaaatt ttttcagctc cggcaaggag
      541 ttacctgatt tttggctata cacacttgcc tgcatcacgt ccttttccac cgtgcgagag
      601 acttcggttg agaaattaaa agaagccggt ttatctttat gttccacggt atgagtttcc
      661 gctgaagtta ccgctgttgc gactgcaagc ggtagaatgg taagaaaatt tttcaatttc
      721 ataatcagtc cttaaaaggg ttaaaaatca tttcttagat ctcacttgcg taggaaagtt
      781 cattcaatcg aaagcacaat cgcctgtgta tcattttcaa tttgccagcg aatattgtag
      841 ccggcaaggt tcataccata aactcgctca ctaggtttcc cttgttgata cgccgggcga
      901 gggtcttggg cgatgacatc ggcaagaaaa ctaagcggct gttcgatgcc aaattttgca
      961 aaatttcggc aaaattcgac agcttgtttt gcctcctcgc taaaacgcac cgctaatctt
     1021 gcttgtggtt tttgctgagc aaaaccggaa accgcttgcg gctcgctatc tgcaaatgca
     1081 atgtaaggct tgatgtcaaa aattggtgtg ccgttaacta aatccacgct gcctaatttc
     1141 aaataaactt cactcccccc cgccactacc gcttctaact tcactttcga taagccaagc
     1201 ggattcggac gatgggtggc acggctggca aagaccccga cccgttcgtt cccgcccaag
     1261 cgaggcggtc tgacggtggc gtgccactct ctttcgggaa tgtgatggaa ttggaaaatc
     1321 aaccaaatat gactaaattt ctccaagcct ctgactgcat cgggggtatt gtaaggcgga
     1381 attaaacgta gaatgccttt cccctccgct accaaattcg gctggcgagg cacggaaaat
     1441 ttttcatcat aggggctttc cacaaaaccg atagggttta attgaatcgg ggttagttga
     1501 tttgaggtag agttcattac gcttttttct gttaaaataa tataaaatat tggacataat
     1561 gatatgaaat tgaggatggt gcaagcgtcc acgcttgtac caattcgttt taccatttaa
     1621 cacaagagtg gacgcttgca ctatcgctta aatccttaac atcattgtaa atgaaattac
     1681 gctaagatta ttctctatga acaaaaattc tctcttttcg atttggttta ccaccgctcg
     1741 cccgaaaact ttaccgctgg cgttagcttc aattattgtg ggttcagcac ttgctcattg
     1801 ggcagggaaa tttgatgttg ttaccacctt actcgcattt atcaccacca ttttgttgca
     1861 gatcctgtct aacttcgcca atgattacgg cgaccacgtc aaaggctctg acaccaaaga
     1921 acgaatcgga ccgctacggg cgattcaaca cggtgttatt ggcggtgaac aacttaagaa
     1981 agccgtgatt atcttaagcc tactctcgtt tgtttccggt gttgccttaa gtgcctatgc
     2041 ttttgaaaca gtgcaggatc tgtttttctt tatcgggctt ggcatcgtct caattattgc
     2101 cgccattact tacaccgtcg gcaaaaaagc ctacggctat ttggggctgg gcgatctatt
     2161 tgtgctgatt ttctttggct ttgtagcggt aatcggcgtg ttctacttgc aggctcatag
     2221 cctacctata atgattctgc tccctgcttt cggttgcgga ttgctgtctg tagcagtgtt
     2281 aaatatcaat aacttgcgtg atattaacca agaccgacaa gctgggaaaa acactctcat
     2341 tgtacgaatc ggtagccaaa acgggcgaaa ataccacgtt gcattgttag tgctagcggt
     2401 catttcttat ctgatttttg caatttctga atttgaacat tggtatagct tcattttttt
     2461 gctgtctgta ccgttactgg caaaacacgc tcgttttgtc tatcagcata aagacccaaa
     2521 agaactctac ccaattctag ggcaaatggc agggctggcg ttgctcacta atctattatt
     2581 tagtttgggc attgtgttag ggtaagttgc aaaattttaa caaaactaaa ccgcttgtaa
     2641 taaaggatta caagcggttg ttttttatct aaatttacaa ttagttatct agttcttcaa
     2701 agaatccatc tttttcgcca cgttgatact ccgaattgag caacttatta ataatgtacc
     2761 aatccgcttc aacctctata tcgccttctg cataagcacc taattcgtat ggcggataaa
     2821 caaaagcaat gccgtgaggg gtaaagtaga attggtcaga aatacggaac tcttttttat
     2881 cagcaaatcc attatagttg ccattttcat ccacacgcac gctactgtaa ttttcccaaa
     2941 gaatgtcttt cagttgaact tgattttttg ggctgattaa atcatttaag ccaatgattg
     3001 cttttttagc taaatctaca ttgatatatt gcgtatagtg cataccgtga gccccaccca
     3061 tataggtgta atgcatcata gtgaatgtag caatattatt gcgttgcccg ataaaagcac
     3121 tggaaatatg atcatcatag ccaatagctg gctcggtttt cacactttct accaaccctt
     3181 gataaaccgc ttcacgatgc tttttaaact cttcttcggt tggatttttc agctttctgc
     3241 cttgttcttc attgtaatca taggcctgat taatcaaaag ctggttaagc caatcaaaat
     3301 tggtttttgg tagagacaca aacgcactta ctgtactttc ttctattgca aattcttgtt
     3361 tcggatcaac ttggtgctta atcacctctt tcttatcaaa gattttcaca atttcaacat
     3421 ttaacgctgt gttttgctga gcgttctcta actctgattt taatttttca ttttctgctt
     3481 tcacccctga aagttctgca acttcagcct ctttttttgc taaatcagcc tgagcatttt
     3541 ttaattctgt gcctaactga acaatttgtt tttcggcttc taataccgtt tgggtcattt
     3601 tgccgtcttc acaagctgaa atcagaaata ctgagccaat taaaagtgct aatagtgatt
     3661 ttttcatctc tccctctttt gtaaaaaata cggtaaatat caccgcttgt tatacagatt
     3721 aaaacaaaat cccctatacc atcaggcata ggggaaatga ttagtcgtct aagaaactgc
     3781 gtaaggtttc cgaacggctc gggtggcgaa gtttgcgcag tgctttcgcc tcaatttggc
     3841 ggatacgttc acgagttacg tcaaattgtt taccaacttc ttcgagtgtg tggtctgtgt
     3901 tcatatcaat accaaaacgc atacgcaaca ctttcgcttc acgcggggtt aagccctcta
     3961 acacttcgtt cgttgccata cgcaggctct ccgcagtggc ggaatccaat ggtaattcaa
     4021 ggctttcgtc ttgaatgaaa tcacctaaat gtgaatcgtc atcgtcaccg attggtgttt
     4081 ccattgaaat cggctctttg gcaattttca atactttgcg gattttatct tccggcattc
     4141 ccatacgttc tgccagttct tccggcgttg cctcacgccc catttcttgt aaacattggc
     4201 gtgaaatacg gttgagtttg ttgatagttt caatcatatg caccggaata cgaattgtac
     4261 gggcttggtc agcaatcgaa cgggtaatcg cttgacgaat ccaccaagtt gcataagtcg
     4321 agaatttata accacggcgg tattcaaatt tatccaccgc tttcattaaa ccgatgtttc
     4381 cttcttggat taaatctaag aactgcaagc cacggttagt gtattttttc gcaatcgaaa
     4441 tcaccaaacg taagttagct tccaccatct cttttttcgc acggcgggct tttaactcac
     4501 cattggaaat acgcccgcca atttcacgaa tttgagcaat ggttaagcct gatgtttgct
     4561 ctaaattctg taagttagca atattgacac ggatattatc cacataatta gctaatttcg
     4621 gtgctgcatt tttcttggca ttaatcgctt tttcaatcca cgcttcgctt aattcgtgcc
     4681 cttggaatgc tttcaagaaa tccgcttttt tcatgccggc atattccact gcatagcgtt
     4741 gaatttgacg ttcttcagtg cgaacctgct tcatcagttt ttgcatagaa gcaaccaata
     4801 agtcaaattg cttcggcacc aaacggaatt cacggaaaat atgagataaa atttcaattt
     4861 gctctttcga ttttttactg gttcgcccgt gtttttgaat gaccaataac actttttcat
     4921 gttggctgcg tagggcttca aatttttcac gggcaagttc cgggtcaatt gagctgtcat
     4981 cactgctgtc tgaactgcta tcaccgtttt catcttcgtt ttcgtcatct acatcaggca
     5041 catcagaaga ttcttcatct tcacctaaag cagcctcaat atcctctaac ggtgcttctt
     5101 ctaaaatatt aggatcgaca aatgctgtca ctaaatcaga aaggcgcatt tcaccggttt
     5161 cgacttgagc atagcaggct aacaggccac ttaaggcttc cggatattcc gcaatcgcac
     5221 attgaacttc gttaatccct tcttcaatgc gtttagcgat atcaatctca ccttcacggg
     5281 ttaaaagctc aactgtcccc atctcacgca tatacatacg cacagggtca gtagttctac
     5341 ctaactctga ttctacggtt gaaagcactt tggtggcttc ttctaccaca tcttcatcgg
     5401 cgatattttc agcgagtagt aaatcatccg catcaggggc agcctctaaa acttggatac
     5461 ccatatcgtt gatcatctga ataatatctt cgatctgctc cgcatccacc aactcttccg
     5521 gtaagtggtc gtgcacttca gaaagcgtta aatatccctg ttcacgccct tgactgatca
     5581 gaagttctaa ttgagattgc tgttctaatt gttgatctgc ttggttttcc atagtctatc
     5641 cgtttattat gcgagcaaaa atagtagtgg attataccac taaatttggt tcgacttcat
     5701 aaattcacaa aaaaattcta tttttctaat ttacacatta ctttgatgct tatgagaaca
     5761 gagaggtgta gcaagagaga taaataactt tctttttaga tagaattaat tattttcttc
     5821 gttcatcact tctgccaaat ctcccctaac ccctctttgc taaagagggg aattattcga
     5881 gcttaaaatt ctactgaaac atcaaaagag catactcttt tttctcatca tcggttaagc
     5941 ccacggttcg gtctttggca attagctctt ctttgcgtaa ttccactaat ttggcgtaga
     6001 gaaaatcgag agtgtcaata aatgttcgtt cgatattttc gggtaactct aaatgatccc
     6061 aatttgcgag ggcttcaaca atcttatatt ggggatcttc ccgataatgt tcaagtagcc
     6121 cgcccatact gatacctgca ttttgctgac aagttgcgac aatatcggtt aataatgtaa
     6181 agccaggatc tttcaaggag gctaaagcac ttacatcggg tacgaatttc acaaaatacg
     6241 gattttgaat caataacgca atcaacagcc gcattggcgt acgtttcatt ttgaccgctt
     6301 gatgtaaagg cttctctgcc gcttttttcg tcggtaacag agcttctatc tgcgttgggt
     6361 ctaaaatgcc taatttctgt cctaaaatat tgcgtaaata cactcgcagc atctcgcccg
     6421 gaatacgatt aattaacggc acagctaaag cagcgagctt ggattttccc tcttttgaag
     6481 agagatccac atccgccagc aaactgtcaa ataagaaatc gcttaatggc ttggcattct
     6541 gtgccacgta ctgctcaaat ccttctttac cttgagaacg aacaaaggtg tccgggtctt
     6601 cgccatcggg caagaaaata aatttcagtt ggcgaccgtc ttgcaaataa ggcaacgcat
     6661 tttctaacgc ccgccaagct gcatctcgac cggctctatc gccatcataa caacagacaa
     6721 tttgctcggt gcaacgaaac atttgctgaa tttgttcacc ggtagttgcc gtgcctaaag
     6781 aggcgactgc attgtccacg ccgaattggg caagggcgac cacgtccata tagccttcca
     6841 ccacgaacag ataatccggc gtttcattga ctttcagtgc ttgatacaag ccatataact
     6901 cactgccttt atgataaatc accgtttcgg gcgagttcat atatttcgcc ccttgctcat
     6961 cattcggcaa cacccgccca ccaaaggcaa ttacccgtcc acgcttatct cgaatcggta
     7021 acattacccg attgcgaaat ttatcgtaaa ttcgaccgct atcatttttg gttaaaacgc
     7081 ccgtatcgag caatttctga atatcttctt gtgattgccc aaaagtgcgt agggctgcat
     7141 caaaggcatt cggagcaaaa ccaatttcaa agcgttcgat aatctcttca gacaaacctc
     7201 gttgctgtaa atagctttgt ggctcaatct ggtgcgataa attctgctgg tagaacttcg
     7261 ccaccgcttc catcaattca taaagattac gcttagtttt aaaattagct tgaggcttgc
     7321 cgtcacgttg aatcacattc tctctcggta cttccagccc gtgcatagct gccagttctt
     7381 caatcgcttc cggaaattcg agtttgtcat aatccatcaa aaaggtgatg acattgccgt
     7441 gtacgccaca gccgaaacaa tggtaaaact gttttgattg gcttaccgaa aaggaagggg
     7501 ttttctcgtg atgaaaagga cagcaggctt gataatctcg cccagccttt ttgagtttga
     7561 cccgactgtt gatgacctca acaatatcgg tgcgagcgat aagatcatca ataaatgaac
     7621 gtggaattgt gcctttcatt gatttcctcc tgctctttga atgatacaag cggagcaatt
     7681 ttgcaaattt tttgcaaaaa acaaccgctt gaaatagaaa cgttaaaaac accaaaaccg
     7741 tgagcctaac ggaatcacgg tttcgatata gtttgctaac cagcctaact aattagtata
     7801 aacgtgtgtt acgtgcgttc tcacggaaat tacgtttagc gtgacgttta gcaagtgatg
     7861 ctttttcacg tttacgaatt gtagttggtt tttcgtagaa ttcacgagca cgaacttcag
     7921 ctaagatacc cgctttttcg caagagcgtt tgaaacgacg taatgctacg tcaaatgatt
     7981 cattttcacg aactttaatt actggcatat gccatcacct cagaaaaata tgattaaatt
     8041 aaaccgcaca caattttggc ggcatagaac aaaaaaggta gtgcattcta ccttaagcac
     8101 tcacataagt aaagtagtat tgtggcataa aaccgccgta ttatgttgca tttttctaca
     8161 gttcgaataa tttctcggtc gaaaaactta atgccggaaa gaccaccgaa gttgccggtt
     8221 catcttctgc caccggctga ataccgatat atttaccgtt ttccaacaca aaaatctgaa
     8281 tagtgcgggt ttcgggagaa acaatccaat actctttcac cccttgttcc tggtaaagct
     8341 cgtatttatc tttcatctcc cttttggtat tacccggaga aaggatttcc accactaaat
     8401 ccggtgcccc tacacagcct ctttcatcta acttactgac atcacaaatt acacaaagat
     8461 caggctgcac tacagttttg acttttccgt tttcatcggg aaaccgaacg tcaaaaggcg
     8521 cagaataaat ctggcattgg tgattgtcaa aaacctctaa aaattttgct gtcatcttca
     8581 tagaaattcg ctgatgaaaa cgagtaggag ccggagacat cgccacaatt ttccctttga
     8641 taatctcaac ccgctcttta aatttccata gcaaataatc agcgtaactg taactttttt
     8701 gtaagtccag ttgatttaag ctggtaattt cgttcattgg taataagctc ataccgcctc
     8761 ctttatcaga aatataaaga tattatgccg aataatccct caaattcaac aaaactttcg
     8821 ctaaaaagag atagataacc tttataccct tagtataaaa tgacagcaat tttcattgtt
     8881 ttaggatatg tcgatgcacc ttaacttcaa aaaaatagac aactcacata ttgctctttt
     8941 ctataatgta ctagtcgcca cattttttgt cactgtttta acctttaaga aaggttatag
     9001 ttatgtgcca atggcattag gtattatcgc tacacttagt ttcttatttt atcgtttcaa
     9061 attaaaacaa aagtggttac ttgataaaga ggataaattt tttattttct cgcttattgg
     9121 ctattttcta acgtttgcaa tgtctgctat ttttaatggc gatggcattc gtgaaattga
     9181 taatcctatc cagattttat tgcttatccc tttaatcatc ctctttagac tttacccgat
     9241 taaaaaagaa actgtattgc actttatacc gattggagcg ttggtagctg gttgtatagc
     9301 aatatatcag aggtttatat taaatcttaa atttcctttc cctgaaataa tgcgtattca
     9361 atcggggaac atagcaatat tattatcact atttagtata gcaattgcct tttattggct
     9421 ttcaaaaaaa agaattaaaa ctggcattgc ctacctggtt tttgccatgt ttgggttatt
     9481 tgccagtatt ctaacaggag caagaggcgg ctggcttgca ttcattccca tttgcttttt
     9541 tatcctattt ttaaatttaa gacagctaaa taagaaagct attttcctga ttctattagc
     9601 acttatatct tcactctcat tttttatctc caaaccagaa tttggcatac aaaaacgata
     9661 taatgcagct aagaatgata ttatcgtatt tttagaaaaa ggaaatagac attcatctct
     9721 gggagctcgt tttgatatgt gggaaaatgc gcttatcgga atccgtgaaa aaccgctatt
     9781 aggacacggt agcacaggct atgaaacatt caaaaagaaa cagattgaaa caaaacaaat
     9841 ggcaaaaaca acgcttaggt ttaatagctt acacaaccaa tacctagaag cctttgtgaa
     9901 aagaggtgta gttggtttta tggcgctaat ggctatttta attattccat ttgttatttt
     9961 tgccaaacga ttaaaggcaa aaaatagaga ggtaaaatgt atggcgatat taggcattgt
    10021 acatattatt tcgcattcat tctttttcct aagccaatca tttttagccc ataattcagg
    10081 ctcattattc tatttctttg tgttaatttt actctaccat ttagtcaaac agaacgaaga
    10141 gataatatga taaaacaata gaagaaagtt gggaatataa ccgaggtggt aacttcatct
    10201 tctaccacag gataaatgtc gggatatttc ctctttttct gcttcttact actaattccg
    10261 attagccaaa taccattcca ctgttttacg caagccactt tcaaaagttt ctttcggttt
    10321 ccagtcgagt tccttttcga tttttgaagt atcaattgca tagcggaaat cgtggccagg
    10381 gcgatcttgc acataagtaa ttaaatcagc atattgtcct acaacaagcg ggttatttgg
    10441 caccatttct tgcaaaatag aacagataga acgaaccact tcgatattgg ttttttcatt
    10501 atgcccgcca atattgtagg tttctccgat ttgccctttt tgcaacacca aatagagggc
    10561 acgaatatga tcttccacaa acagccaatc tctgacctgc aagccgttgc cgtaaacagg
    10621 tagggattta ccttctaagg catttaaaat cattaacgga ataagttttt cgggatattg
    10681 gtatgcccca taattattgg aacagttggt gatgacagtc ggtaaaccgt aggtgcgatg
    10741 ccaagcccga gccagatgat cgctggcagc tttagatgcc gaataggggc tacttggatt
    10801 ataaggggtt ttctcggtaa ataattcctc gcttttggct aaatcaccat agacctcgtc
    10861 ggtcgagata tgtagaaagc gaaacgccga tttccttggc tcaggtaacg tgtcccaata
    10921 ggctctcgct gcctctaata aagtataagt gccgacaata ttggtttgaa taaaatctgc
    10981 tgccccagaa atagagcgat caacatggct ttccgctgct aagtgcatta ccacatcagg
    11041 ctgatatttg ccaaaaatct cagaaatttg accgcttgca caaatatctg ctttttcaaa
    11101 atggtagcgt tcgtgatgtg cgacactctc caatgaggct aaatttcccg cataggtgag
    11161 cttatccaca ttgataactg tgtgtggagt atgttcaatc agatagcgaa ctaatgcaga
    11221 tccgataaaa ccggctccgc cagtgatgag gatgatcata aaggtgaata tgaaatttga
    11281 ttcacgaaat ttcgtttgta tttaatatgg ctttgaatat tcttttcagc gtctttttta
    11341 tcccaaattt gcaattccca aggataataa aagttacttg aattcttaaa ataaatatga
    11401 atacccacat attcatcttt atctctcaaa taccaatttt ttagtccata tttttcttgc
    11461 caataatcta atgactccat tacttgctga atcacttcac tagaaacaat aatacgagct
    11521 ccaaaaatat cgttgagaat gctatttact ggataacctt cactacgctg tttaaaacgc
    11581 tcgattttat ctaaaatcga ttctgaggtt tttactcggt aaaaataaga aattccctct
    11641 aaatctgcac gatataaata atcattaata gattcgtgta aattaagacg ataagctaaa
    11701 atatgtggcg taggcacttt ggcaaaagta tgcttaagat tcactttttc aatcttacct
    11761 gtttcaaagt agtctttcga aaaagctaag tgcaacttgt tgatttcatc aatgagtttt
    11821 tctattttct caagcatgct attcactctc taatttatta tcttgcccca aatccagtta
    11881 gcattgtttt caatgtttta aaaataatta aaatatcaag ccataaagaa aaatgcttaa
    11941 tatagtagaa gtcatgttct acttttactt ttgtatcatc aacatctgcc gcataacctt
    12001 ggacgacctg agcccaaccg gaaatacccg gtttaacgat atggcgataa ttatagaatg
    12061 gaatttcctc ttcaaattta tctacaaagg ctgtctgttc tgggcgagga ccaattaagc
    12121 tcatatcacc ttttaataca ttaaaaaact gaggtagctc atcaatacga gttttacgga
    12181 taaatttccc aatacgagtt accctcatat ccgcactcga agcaaactgt gcatcatctt
    12241 tttcggagtc tttacacata ctacgaaatt tataaatatt aaactccttt ccaccttgcc
    12301 caacgcgttt ttgaataaac attgctccac cttcactttc taagcgaata gcaatagcgg
    12361 tgattgtcat aataggtaaa gtaatgggaa acgtagcaac aatcaatgca atatcaatga
    12421 tacgcttcac taatgagtaa attggagaag gaagtaatga acctaagtca ttttcataca
    12481 tatggcgaat tttgacacgc ccggttaaag attcctcaag ctggcgaacg ttatatacag
    12541 gaataccatt caatgtacat ttcgctaaaa atttatgcca ttctgcggac aaatcaggat
    12601 agtgtaaatc taccgcaata gagttaattc tcttttcccc caatattggt tcacttaatt
    12661 gaatccactc tacattctga attcggttca aattgatagc tcttccgaat ggaaccaaag
    12721 cgattttagg gataatccaa cttcttgaaa gaaaataacc aataaaacaa aatagaattg
    12781 tgagtataaa gctcgtacct agcaatgtat tagaataatc aaatctaaat attgttaaca
    12841 cgattaataa aaccgcatac caagaaataa cggtggaaaa aacaaaactt gctgacttat
    12901 tacctggata tttcattaac actcttaagc tgatagctat cattacaaag gcaagagaag
    12961 tcgctataat cgtattagaa cgagtttcaa tagaattaaa tttctctccc caaaaataga
    13021 tagcgggtaa gacacttgag aacataacgc ccaaaatact ttgaacagct gtactaaaaa
    13081 agatacgttc ataccaacga gagtgtcttt tcttttgctg aatgctcata atttatcctt
    13141 ttcttaattt cttataaagt cttcctaaaa gagatctagg caataaccgt ggtaaggctc
    13201 taagtataaa aaacatgctt gatgagatta ttgattgtaa tcccaactct tttttcaatt
    13261 ttgctaattg gtactcactc ttaatgtaat tccacccttt acgccgagcg tacattgcag
    13321 aacctgaacg aacatttacc agtacatctg gtaaattata cacctcatat ttctgtgtaa
    13381 taactcgtaa ccataaattg taatcttcca tataaagatg atgctgatag cctccaacat
    13441 gctcgatcac agatttttta taggctactg ccatatggtt aaaaggattt cttagtaatg
    13501 ctgaaatacg aatttcatca tcattaatgg gcacttgttt tatacccaga gaattagaca
    13561 ttgtttcgtc aaattcttca acttgtcccc ccaataaaac cacatcagga tttttactaa
    13621 taaaatcaat ttgcttttca aatcgagatg gtaagcaaat atcatcagta tccattctaa
    13681 acacccactc attagagcag tgttttaagc cttcatttaa ggccggccta aacccacatt
    13741 ttgtggtaaa ctaacaacaa caagcggtaa tttttcagta aaatgttgca caattcgctc
    13801 taaatctcca ggaattatac catcaaaaac cacaacaatt tcacttgctg gtaaagtttg
    13861 ggatactaaa gaatctaaac acatctctaa ataagctggt ttttcttta
//
LOCUS       DANLUA010000048        13788 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLUA010000048 DANLUA010000000
VERSION     DANLUA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(25..1564)
                     /locus_tag="PXB44_001046"
     rRNA            complement(25..1564)
                     /locus_tag="PXB44_001046"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1767..1843)
                     /locus_tag="PXB44_001047"
     tRNA            complement(1767..1843)
                     /locus_tag="PXB44_001047"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1807..1809),aa:Pro,seq:tgg)
     gene            complement(1868..1943)
                     /locus_tag="PXB44_001048"
     tRNA            complement(1868..1943)
                     /locus_tag="PXB44_001048"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:His,seq:gtg)
     gene            complement(1973..2049)
                     /locus_tag="PXB44_001049"
     tRNA            complement(1973..2049)
                     /locus_tag="PXB44_001049"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2013..2015),aa:Arg,seq:ccg)
     gene            complement(2208..2930)
                     /gene="rffM"
                     /locus_tag="PXB44_001050"
     CDS             complement(2208..2930)
                     /gene="rffM"
                     /locus_tag="PXB44_001050"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL5281541.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(2920..4191)
                     /locus_tag="PXB44_001051"
     CDS             complement(2920..4191)
                     /locus_tag="PXB44_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL5281542.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(4184..5254)
                     /locus_tag="PXB44_001052"
     CDS             complement(4184..5254)
                     /locus_tag="PXB44_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL5281543.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            5441..6862
                     /gene="glnA"
                     /locus_tag="PXB44_001053"
     CDS             5441..6862
                     /gene="glnA"
                     /locus_tag="PXB44_001053"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL5281544.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(6906..8049)
                     /gene="rffA"
                     /locus_tag="PXB44_001054"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(6906..8049)
                     /gene="rffA"
                     /locus_tag="PXB44_001054"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(8036..8680)
                     /gene="rffC"
                     /locus_tag="PXB44_001055"
                     /gene_synonym="wecD"
     CDS             complement(8036..8680)
                     /gene="rffC"
                     /locus_tag="PXB44_001055"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL5281545.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(8677..9924)
                     /gene="wecC"
                     /locus_tag="PXB44_001056"
     CDS             complement(8677..9924)
                     /gene="wecC"
                     /locus_tag="PXB44_001056"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5281546.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(9965..10777)
                     /locus_tag="PXB44_001057"
                     /pseudo
     CDS             complement(9965..10777)
                     /locus_tag="PXB44_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
     gene            complement(10788..11852)
                     /gene="wecA"
                     /locus_tag="PXB44_001058"
     CDS             complement(10788..11852)
                     /gene="wecA"
                     /locus_tag="PXB44_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL5281547.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            11994..13274
                     /gene="hemL"
                     /locus_tag="PXB44_001059"
     CDS             11994..13274
                     /gene="hemL"
                     /locus_tag="PXB44_001059"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL5281548.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3975 a         3318 c         2778 g         3717 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat tcccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgtcc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcca aatttaaacc
     1621 taattaaaat taagttaaaa gaaagtaatg aatttctagt tagcacctat taagacttca
     1681 aaattaaaaa aatgtttttc aatcaagtca atcaacaagc gcccacacag attgtctgat
     1741 aaattgttaa agaacaaaaa ataaattggt cggcgagata ggatttgaac ctacgaccca
     1801 ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgtctgata ctcacaagag
     1861 tattgaatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc
     1921 tctaccaact gagctatagc caccattgtg ttgatagtgc cgcattgaca tctggcgcgc
     1981 tcgacaagat tcgaacttgc gacctttggc tccggaggcc aacgctctat ccaactgagc
     2041 tacgaacgct tcgatttaca tcaattcaac ttaatgcgtc gcaacggagg cgtatattat
     2101 agatttcact ttcccttgtc tagcactttt taaaaaaaat gtaaaaaaat gtattgtttg
     2161 gttttatttt attcaaaacg tttaataaat atcaaaaaat gcgtttttta gagctgattt
     2221 gtcagatagt aataggcata ttttagtaaa tttaattgtc tttgccagcg ggttggctgt
     2281 ttcaatagtc gataaagcca ttccagcccc aaattttgcc aaattttagg tgctcgtttc
     2341 actttaccgg caaacacatc gtaagtcccg ccaacaccca tatataaggc ttgtggatat
     2401 tgttgttgta actgttggat aaaaagttcc tgttttggag ttcccattgc cacactaata
     2461 aacttcgcgc cacttgcttt aatttgctct ataacgtcct gttcttgctc cgccttaaaa
     2521 tagccattct gcctaccgac aatattcacc ttccattgtg ccaattttgc ttccaccttc
     2581 gttagggtat ccgccgagct gccgaccaaa aacaccggca catttaacgc tccggctctt
     2641 tgcattaagg tttcccataa atcggctccg gcaattcgct ctattttata gtttggatat
     2701 ttttttcgaa tcgattgaac aacactgatg ccgtctgcat agttgaactc ggcttcatct
     2761 atcactcggc gtaacaacgg atttttttcc gacagaatca ctttctcagc attaattgcc
     2821 actaatcttc cgaactgaat ccgcttttca ttcatcagcc aatttgcaaa aatttcccga
     2881 tttttgaccg cttgtaacgc aatgccccga atgatcactt tatcgaacat tattattttt
     2941 ccccattgaa aggattttcg ccagcaaata gcaagccata aatatcagcg tgaaaaagca
     3001 aaaacgggaa acaaatgcat cgattccctc tcgcactaat acgatcaaat tgaataaatt
     3061 gccgaagcag taggcttgca acaaggcgga attgcacgct ttggcgtaag cgaataggtt
     3121 atctaaggct ttaatcatgg cagctactat ccccattccc aacgccataa cgctaaatcc
     3181 gcccataata tagaaagagc cgagtaaggt aggcgaaata gctaaccctg attggttgcc
     3241 taaaatcact ttggtaaaat aattcgccgt attccagacg atctcagggc gttcttgcca
     3301 taaagattgt gggatatata catagaaatc ccgcacaatc ggcattaaac cttgatattc
     3361 cacctcatta gaaaatacac gagccaaatt ttcccaaggc gaaaaagtat ctcggcttaa
     3421 atataagaag gtgtaaatca tctcgctacc ggaaagggta agtttgtaac gggctagagc
     3481 gaggaagaac atcgccacca cgccgattag gcttacggca atcacctttg ataacgacag
     3541 atagccttgc tgcaatccaa tcaataagaa aaagccaacc gctagtgcta aatttgccca
     3601 tgtgccgccc actgccacat agcttaatat gccgaatgcc ccaccaacaa tcagtaacgc
     3661 ccaccacgct gttttattct tatataggaa gcaaaaaatt aacaacgccg gcaataaaaa
     3721 gtagaaaaat cgttttaatg ccgaaagtat gaccctatct ttggcaaaca gttggctata
     3781 tttttcgagt ttgaaaagca gaaaaccgtt taaataaagg aaatatagca gggcaacaac
     3841 tgccacagcg gcaaggatac aagcggtcgt ttttgcctga aaatttgcaa caagagcagt
     3901 tggttgcgac acagcactcc acaagcgtga atagacaacg taataaatca aatagccaac
     3961 acctgctgcc gtaaaaactt gccattgtgt ttttagctcc ggcagcgggt aatcaaagcc
     4021 aaatgccatt attaacgaaa acggaaaacc ggcgtaaaaa gtaacaaaat agagcaaact
     4081 aaacagtaaa tggaaagaaa tccccgattt ctgataaagt tgataaagta agctgaaaaa
     4141 tagcccgaca gaaacaagat aaaatccgcc aagtggtatt aaattattca ttttgcatta
     4201 attcgattaa tcgctgttgc catagtttag tgtagcccgg agcaaaaaac gcaatgcggt
     4261 ttttatctat gctctttagt tgttgtttgg ttgtttgaat ctgtgaagca gtcagatttt
     4321 ccgccaacaa ataaggcact ttttctgcct gtagatccaa cccaaacgga ttggctgaat
     4381 gtaacacaca aggtacatta tggctaatca aaaggcttat cgtgccaatg ccttgctggc
     4441 gtttaaaatt gaaatagccg agatcacaat ggctcaataa cgtaacatac tcttcaaagc
     4501 tcagcttctt ccgcaaaatt tgcaaatttt ccgccaaaaa taaccgcttg ctataggctt
     4561 ccacctgccg aatatagctt tcattatttt ccgggtagcc cattggtatg ataatccgca
     4621 cattgttccc tagtcgttga tgaatctcgc ttaaggcttg aataagctca ttcgctggat
     4681 cgcctgaatt gccgagcaat atcgtcaagc gatcattttg tgtggggcta atttttattt
     4741 gatcacctaa tttcgtgggg aaatagatta aatgatcctg ttctgatctt gtacgcatat
     4801 ttcgccacaa atgatccaga tcgcctcttg ttgcccaaac ctgtaagatt ttttgttggg
     4861 caacacgccg aaacgggtaa aacagcttaa atttccacga agttgccatt tcatataaat
     4921 ccgcccccca aatatgccaa atcaggttgg tggtcggcaa tctgcctaag gctatcgcca
     4981 gccaaatcca agggttaaac tgcccgtgta acagaaaaca agcggtcgga ttttgccgtt
     5041 tttttgcaat taaggcgttc gcaatcgctt tttgtgaaga gaactgctta atccgcaaat
     5101 gaggaaaagc agtttgcagt gaaggcttgc ctaccacata aaaaaaatgt tcggagtctg
     5161 gcaataacgg caaaagctct tgctgaaaaa aggttaaaac ctgttggtta tgatgctcaa
     5221 tctcggagcc tagaatatga taaatcggca acattacacg cttttctctc cccaccatac
     5281 aaacaatatg caagcggtaa gattttagca aaaatttgca acattactgc aacttctttt
     5341 agctttgaaa cactaaaaaa catcacacac ttggttaaaa aatgctatga taaccgagct
     5401 tttttattat tcatttatcc caacgaggac ttattttact atgtcaaacg caatcgctaa
     5461 tgtatctaaa ctgattcaag acaatgatat taaatttgtc ttattaaaat ttactgatat
     5521 taaaggtaaa gaacacgggg tttcgctccc tgtcagctta gttgccaacg atttggaaga
     5581 cttattcgag gaaggcaaaa tgttcgacgg ttcgtccgta gaaggttgga aggcaattaa
     5641 caaagcagat atgttgttaa tgccgattgc tgaaaccgct gtagttgacc ctttcgcccg
     5701 tattcctacc ctttctattc gttgttccat ttacgaccca aacacaatgc agtcttatga
     5761 ccgtgaccct cgctctattg caacccgtgc cgaaaactac ttgaaatcca ccggtatcgc
     5821 cgatagcgta atgtttggac ctgagcctga atttttctta ttcgatgatg tgcgttttag
     5881 caccgaaatg aacaatgtgt cataccgaat tgacgatgta gaagcggctt ggaataccaa
     5941 ccgtaaattt gaagaaggca acaaagccta tcgtccgttg aaaaaaggcg gctactgtgc
     6001 ggttgcaccg attgataccg cccacgatat tcgctccgaa atgtgtttat tgatggaaga
     6061 gatgggttta gtgattgaag ctcaccacca cgaagttgcg acagccggac aaaatgagat
     6121 cgcaacccga ttcaacagcc tgaccttaaa agccgatgaa acccaaatct ataaatacat
     6181 cgtgcaaaat gttgccgtag aacacggaaa aaccgcttgc tttatgccaa aaccgttcgc
     6241 cggcgacaac ggctcgggaa tgcactgtaa tatgtcatta agcaaagacg gcaaaaatgt
     6301 gtttatgggc gataaatacg ccggcttatc tgaaactgcc ctctactata ttggcggtat
     6361 cattaaacac gcaaaagcat taaatgcctt tactaaccca accacgaact cttataaacg
     6421 tttagtgccg ggttttgaag caccggtgtt attggcatat tctgcaagta accgctctgc
     6481 ctctatccgt attccggctg tgaccagtcc gaaagcgacc cgtgtagaag cacgcttccc
     6541 ggatccaatg gcaaacccat acctttgctt tgccgccctg ttaatggctg gcttagacgg
     6601 tgtgatcaac aaaatccacc cgggcgatgc aatggataaa aacttatacg atttaccgcc
     6661 ggaagagtta aaagatattc caacggttgc ctcgtcttta gaatccgctc ttgacgcatt
     6721 aagcaatgat tttgaattct taacacaagg tggcgtgttc acgaaagact ttattgatgc
     6781 ttatatcggt attcgtcgca aagaggtgga acgtgtaaat atgactccgc acccggtaga
     6841 atttgaactt tactattcat agtaacaaca tatagcaaaa aacggctgaa acactcagcc
     6901 gttttttatt gattgaagaa agcaagcact gcttgaataa cacgctcttg ctcctgctta
     6961 ctcaaattat aaaacagtgg caatcttacc aaccgttcgc tttctaaggt agtaaagcga
     7021 tcatcacctg ctaatcgacc taatttctgc cctgccggac tgctgtgcaa cggcacataa
     7081 tgaaacacgg ttaagatatt ctgtgccttc ataaaatcaa taaaagcagt tctctgcttc
     7141 aagtcactta gctttaagta gaacaaatgc ccgttatggc gacaatattc gccaatgcga
     7201 ggaagctcgc acttagctgt ttgggcaagt ggcaaaaagg cttgatagta attgtgccaa
     7261 agggttaaac gctgttggtt gattttatct gcctctataa gctgagcata caaataagcc
     7321 gcttgaattt ccgacattaa aaaacttgaa ccaatatcct gccagcgata tttatctacc
     7381 tcaccacgaa aaaattggct acggttagtg cctttctctc tgatgatttc cgctttttcc
     7441 agcaaatgcc tcattaatca cgatcgcccc accttcaccg ccagcggtat agtttttggt
     7501 ttcgtgaaaa ctatagcaac caatatgccc gatggaacct aacgccctac ctttataaaa
     7561 cgaattgact gcctgtgccg catcttctac cacataaagc ccgtattttt cggctgttgc
     7621 cattattgaa tccatatcac aagcaatgcc ggcataatgc accggcacaa tcactttggt
     7681 ttttggggta atggcttgct caattttgcg ttcatcaata ttcattgtat ccggacgaat
     7741 atccacaaac acaatctttg ccccacgcaa cgcaaacgca tttgcagtag agacaaaagt
     7801 atagctcggc ataatcactt catcgtccgg ctgaatatcc agcagtattg ccgccatctc
     7861 taacgcagcc gtgcaagaag gggtaagcaa cactttgcta cgggccaggt tttcaagcca
     7921 ctgctcacat aagcgggtga atttaccacc gcccgaaagc tgccctgttg ccatcgcctg
     7981 ttctatatag gcttgctccg cccccacaac cgggggttga ttaaacggaa taaaatcagg
     8041 attgttcatc atcgtatttt tccaaagcag ggtaaaacca ataatacacc gccttaatgc
     8101 ttgcaccaaa cgcttgataa aaacgaatcg ctttctggtt actcagctga gtggtaatca
     8161 tcagtttttt cgcattttgc tgtttcgccc acgctatcgc agcatttaac aattccgagc
     8221 caataccttg ttgtcggcaa tcaggcttta ccgccaataa tccgattttc gcttgctgtt
     8281 gggcaagtaa gcgaaccgtt accgcccctt gaatttgccc gtcagcattg cattgaacca
     8341 agccaatatc atcaaacttg ccttttaccg cattttcaat ccaagtccgg taaaagcgtt
     8401 gattctcttc ccttgaaaaa tagggcgaac gaaaccggct atcgggaaat gccgaaccaa
     8461 acaataagtt caagtcgggc aaatctgcca tatccgccgg gcgacaagcg gtcatatttt
     8521 cagaaaatgt tgcaagtgcc agctcaaatt ccaattctcc ctccacaagt acaaaacctt
     8581 gtttttgcaa cctagcgatt tgcctaaaat catcagccgc tacttttact tgcacaattt
     8641 ctctaccgaa aaaatcggac agccattgat ccggcttcat cttacccaca ctcctcgaca
     8701 atcaataata tgccgttgcc tgatctgttc ccttgaaatt tgcttaaatg aagtgtgatc
     8761 cactaacaag actaaaatgt ctgcctgcga aagtgcggtg ttgagatcga ctaaggtgat
     8821 tttttcgcca gccagctgtt gaatatttgg ctcaaccgcc aacaggctac cctgatgcca
     8881 agcggcaagt tgccgggtaa tcgctaatgc cgggctttcc cgcaaatcat caatattcgg
     8941 cttgaaagct agccctaaac aagcgatggt tagctcactt ggcttacaat cttgctccat
     9001 cgcacagtct gcgaccgatg ttttcacttg ctcgattacc cattgcggtt tgctgtcatt
     9061 cacttctcgt gccgtgcgaa taaggcgagc ctgttggggc gatgtggaaa cgataaacca
     9121 cgggtcaacc gccaaacaat gtccgcccac ccctgctccg ggctggagaa tatttactct
     9181 cgggtgaaga tttgccaatt caattagctc ccacacattg atcttcaacg catcacaaat
     9241 cattgaaatt tcattggcaa aggcaatgtt cacatcacga aagctgtttt ccaccaactt
     9301 gcacatctcc gctgtgcgag ctgtggtggt gacacaacgt ccttttgcaa aaatttggta
     9361 taaatcgacc gcttgttgac tgcattgttc gcttaatccg ccgataatgc ggtcattttc
     9421 aaccaattcc gtcattatat tacccggcaa aacacgttca gggcagtagg caatagacac
     9481 atcggctgga aaggctaaat cagggcgagc ttggcttagc cattcggcta aattttctgt
     9541 tgtaccgaca ggcgaagtgg actccagcac caccaaattc cctttacgca aacaaggggc
     9601 aatcatagcg gcggcatttt gaatataagt gagatcgggc tgcttcccgc aaagtggggt
     9661 tggcacggcg atgataaaag catcggccgg ttgcggtttg gcagttgcaa aaaaattacc
     9721 ttttttgacc gcttgtgtga aggcagtttg taaatccggc tcgataatat gaatgttgcc
     9781 ttgattaagg cttgccacca cttgcggatt aatatccacc ccaattactt gcttgccggc
     9841 attggcaaaa acgactgagg tcggcaagcc gatatagcct aaccccagaa tacaaatagt
     9901 gttaaattca gttttactca tcatcagtta caaagagaaa tggcacacta aattgtgtgc
     9961 cattttagat taaagttccg caggttgagc tttcgctttc accgcaaaac tgagcataaa
    10021 gcccagcagt aagccactta atgccccaat catcagccaa aataggcgga tcaggcgatt
    10081 ctgatttaag tggaacgcta ggcgatttaa ccaagcgata agctgtaagt tggttgtcta
    10141 acggctgaac agaacgcatc atttgcagtt tagctgccca atcttgtgag ccttgcttac
    10201 cgttaatatt caactccgaa gcagttttaa tttgctggaa caagactttc catttcgcaa
    10261 ttaaatcggc gtttaattta tctcgagctt aggtgtttac caacggaata aaatcggcta
    10321 ataatttttg agcctcttcc gcattttctg caattaagct gacattatct gccgaagttg
    10381 ccatatcttt ttcaaaacgg aattgttcaa ctaattcagc cgccaccacc gccggtgatt
    10441 tctttgcaac ctgagctttt aatttcaccg cttctgtttg tagcaaatag gcttgaagca
    10501 agtcggcgga cgtgaggttg cgtttaaatt ccgcataaac ctgctcactt gcctgttgct
    10561 gtgccgtttg gctattcgct tcttggctat tactattcaa aaaatggtag gtggaaagta
    10621 acgagtaata attacctaaa tcgggaatag tcggttgctc aaattgagcg gtcactttcc
    10681 atttttcagg ttgagtataa ctcagcccat aaccggcacc tgccccgata gcagcaaata
    10741 aaattagcac taaaagtcga taaaataagg ttttcacaac atatccttta aagttgtttg
    10801 ctacgttttg cacggcgttt tagacggcga acaaaacggg ttacacgcca agcgtgggta
    10861 atggaatagg cgtataggaa aaacagcccg ataaacatta gcatcatcac ccactgattc
    10921 caataataca cctcgcctaa taccccaatg gtggaacaaa atcccgccca gagggtaatc
    10981 accaataatg cttggcgaga ggtaagccct gctcgcatca ttaaatggtg taggtgcaag
    11041 cggtctggtt tgaacggact tttacctttt ttcaaacggc ggataatcac cgcaaccata
    11101 tcaattaacg gcacggcaat cagccacaaa ccggtaatcg ggctaattgg cgagccttgc
    11161 ccttgggtgc ttaacagcag aatccaaata atggtaaagc cgatcagcat actgcctgag
    11221 tcgcccataa acactttcca tttcgcccca aacaggctca aattgaacat tgcatacggc
    11281 aacaacacaa agataagggc aaagcaccaa tagcccaaac ttggttcgcc attaatgaac
    11341 attaaagtgc caagaccggc aaaactcacg ctggaaagcc ctgctaataa gccatcaatc
    11401 ccatctacca tattaaacgc attaataacg ccaatggtga taaacacggt aaagacaatg
    11461 ccgagccaac ctaattctat actaaagggg gcaataattt gccctaaact gctgagcgac
    11521 aagccactgt aaatcatcgc cccagctagt gctgcttgaa tcccggcacg cagtaatggg
    11581 ctgatgtcaa atcggtcatc taacacgccg ataatcagca aaatagttac cgctgtcaga
    11641 tacagttccg gcagccgcat atcttgccac tgcataaaat agaaagtgag attgccgata
    11701 aataatgcaa taccaccgat taacggaatc aacccctgat ggcgtttacg gaagttgggc
    11761 ttatctacca aaccaacaaa ttccgccacc ggacgcatca caatcagggc taaaaaagaa
    11821 acaataaata cggttaaaaa agtaagccac atacagggat tgcctcaaaa aatgagtcat
    11881 aaaataggaa tgcagaatag cataaggcaa caaaatcgta aattttttag cgaaaacaag
    11941 ggcttttcag tacaataaca ggcaaatttt tacatcaaaa gaaaggaatt gatatgtcta
    12001 tttcagaaca actgtttgaa caagcccaaa aagtgattcc gggcggtgtg aactcccctg
    12061 tgcgtgcatt taaaggcgtg ggcggcacac cggtttttat tgaaaaagcc gaaggtgctt
    12121 atattaccga cagcgacggc aaacaatata tcgactatgt cggctcttgg gggccaatgg
    12181 tactcgggca taaccacccg gcgattattg atgcggtgct aaaagccgtg ccaaaaggct
    12241 taagtttcgg tgccccaacg gcagcagaaa tcacacttgc cgaattagtg tgcaaattag
    12301 taccttctat tgaaatggtg agaatggtga gcagcggaac agaggcaaca atgtctgcca
    12361 ttcgtttggc tcgtggctat accaaccgag ataaaatcat caaatttgaa ggctgctacc
    12421 acgggcattc cgattctctg ttagtaaaag ccggttcagg tgctttaacc ctcggtcagc
    12481 caagcggccc gggcgtaccc gctgatttcg ccaagcatac gcttacttgc acctataatg
    12541 atttagattc agtaaaagca gcatttgagc aatacccaaa tgagatcgct tgtttaattg
    12601 ttgagcctgt tgccggcaat atgaactgca ttccgccaca agaaggcttt ttacaaggct
    12661 tacgtgatct ttgcacacaa tatggtgcgg tatttattat tgatgaagta atgaccggct
    12721 tccgtgtcgc actcggtggg gctcaatctt actatggggt aacaccggat ttaaccacac
    12781 tcggcaaaat tatcggcggc ggaatgccgg tgggggcatt tggtggtaaa aaagagatta
    12841 tggaatacat cgctccaact ggcccggttt accaagccgg cacgctctca ggcaacccga
    12901 ttgcaatggc tgcaggtttg gcatgcttaa ctgaattatc aaaagcagga aatgaaacca
    12961 ttttagcgga aaaaaccaaa accctcgctg aaggctttaa agctcttgca gataagcaca
    13021 atgtgccatt aacggtacaa tatgtcggcg gaatgttcgg cttattcttc accgaacaaa
    13081 aagcagtaaa caacttccaa gaagtaatga aatgtgatgc cgccaaattc aaccgtttct
    13141 tccacttaat gctggaacaa ggcgtgtatt tagccccatc ggcattcgag gcagggttta
    13201 tgtctttagc tcacagtgat gaagatattg ctcgtacgct agaggctgcg gataaggcat
    13261 ttgctcagct ttagaagaat gacaagcggt tgggtttact caataatttg caagtttttg
    13321 agggaatttg accgcttgta gtgcaaattt ctcttacgat agcaattatg atggtgttcg
    13381 tctctgacga gtgcttatac aaactttcat tatgcttaaa caagcacccg tctggagacg
    13441 ggcgctatcg agggaatagg attgataaag caatatatgg gcttccaaca cttgcaggca
    13501 aggacaagtc ttacactttc taatattaca aatatatttc tttctttaat ctataccata
    13561 aaggtttaag ttggttcttt ataactttgg aatccataga tgaataagct ctctttccat
    13621 aagagataaa attagatttt ccgaaatgag aatacttaga aaatagcttt cttttataaa
    13681 tgcctttgat agctacccct cgtcttattc ttatcctatt atatttaccc tgagtttgct
    13741 tagctagact aacccctcgt ttcattttat ttgaatatcg tgtaaatg
//
LOCUS       DANLUA010000049        13741 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLUA010000049 DANLUA010000000
VERSION     DANLUA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(51..767)
                     /gene="rph"
                     /locus_tag="PXB44_001060"
     CDS             complement(51..767)
                     /gene="rph"
                     /locus_tag="PXB44_001060"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL5281549.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1048..1911
                     /locus_tag="PXB44_001061"
     CDS             1048..1911
                     /locus_tag="PXB44_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL5281550.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            1920..2810
                     /locus_tag="PXB44_001062"
     CDS             1920..2810
                     /locus_tag="PXB44_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL5281551.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(2842..3102)
                     /locus_tag="PXB44_001063"
     CDS             complement(2842..3102)
                     /locus_tag="PXB44_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL5281552.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            3332..3751
                     /gene="rbsD"
                     /locus_tag="PXB44_001064"
     CDS             3332..3751
                     /gene="rbsD"
                     /locus_tag="PXB44_001064"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL5281553.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3761..5257
                     /gene="rbsA"
                     /locus_tag="PXB44_001065"
     CDS             3761..5257
                     /gene="rbsA"
                     /locus_tag="PXB44_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL5281554.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            5267..6235
                     /gene="rbsC"
                     /locus_tag="PXB44_001066"
     CDS             5267..6235
                     /gene="rbsC"
                     /locus_tag="PXB44_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL5281555.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            6258..7136
                     /gene="rbsB"
                     /locus_tag="PXB44_001067"
     CDS             6258..7136
                     /gene="rbsB"
                     /locus_tag="PXB44_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL5281556.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            7257..8186
                     /gene="rbsK"
                     /locus_tag="PXB44_001068"
     CDS             7257..8186
                     /gene="rbsK"
                     /locus_tag="PXB44_001068"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL5281557.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            8200..9210
                     /locus_tag="PXB44_001069"
     CDS             8200..9210
                     /locus_tag="PXB44_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5281558.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(9246..9875)
                     /locus_tag="PXB44_001070"
     CDS             complement(9246..9875)
                     /locus_tag="PXB44_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5281559.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            10073..12232
                     /gene="rlmKL"
                     /locus_tag="PXB44_001071"
     CDS             10073..12232
                     /gene="rlmKL"
                     /locus_tag="PXB44_001071"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL5281560.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            12409..12888
                     /gene="trmL"
                     /locus_tag="PXB44_001072"
     CDS             12409..12888
                     /gene="trmL"
                     /locus_tag="PXB44_001072"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL5281561.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(12982..>13741)
                     /locus_tag="PXB44_001073"
     CDS             complement(12982..>13741)
                     /locus_tag="PXB44_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL5281562.1"
                     /translation="DKWYFSIIDVIAILTESLDPSAYWRKLKQRLKAEGNETVTNCHG
                     LKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKVGYERLQEINDPSQSL
                     DRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQDFAFLTNVIHQEWSG
                     LSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESMQAVGVDENAQAGKQG
                     GNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         3964 a         2698 c         3197 g         3882 t
ORIGIN      
        1 tttgtcagat gtagatttta tttaaaaaga aaatcaaaaa gagaagtgaa ttattcagct
       61 aacgctttac gttgagcttc aactagctgg ctaatgcctt gcttcgctaa atctagcaat
      121 gtcagtaatt cttggtgcga gaacggttcg ccttctgccg tgccttgtac ttccactaaa
      181 cgaccatctt cgaccatcac cacgttcata tcagtttcag cgttggaatc ttccagatat
      241 tctaaatcgc acaccgcttc gcctgctaca ataccgacag aaattgctgc gactaaacct
      301 ttcattgggt tagtttttag tgttccatta gctactaact tattcattgc atcgtacaat
      361 gctacgcacg caccggtaat tgaggcggtg cgagtgcctc catcggcttg aataacatca
      421 caatctacgg taacggtacg ttcgcctaag gcttttaaat ctaccaccgc acgcagagaa
      481 cgggcaatca agcgttggat ttccattgtg cgtccgcctt gtttgccttt tgccgcttca
      541 cgctgagtgc gagagtgggt ggctcgtggc aacataccat attctgcggt aacccaacct
      601 tgttgctgac cttttaaaaa gcgtggcacg ctttcttcta ccgtagcgtt acacaatact
      661 ttggtttcgc caaactcaat taataccgag ccttcggcat agcgagtgta gttacgggta
      721 attttaattt ctctaacttg gttgtttgct cgattatttg gacgcatttt cttttccttt
      781 tgctaaaatt tttaccattt tagcaaagaa cacagtaaaa tgtgattttt ctttagcaaa
      841 gaatttacat agccctcgcc ccttgtggga gagggacgga tttttcttcg ttcagaagaa
      901 aaatcaggga gaggggtaag cggttatttt tatctcaaat tttacaaatt cctccctctc
      961 tctgaaaaat atttctgaac gaaatatttt tcggtctctc tccctcaagg ggagagagct
     1021 ttacttataa aaacacaagg aataccaatg atttatagta tgacagcatt tgcccattta
     1081 gaaatgaaaa aagagtgggg caatgcggtg tgggaaattc gctcggtaaa tcaacgcttt
     1141 ttagaaacct attttcgttt gccggagcag tttcgcaacc tagaaatgac cttgcgtgag
     1201 cgtttacgtt ctgcactgac acgaggcaaa gtagagtgta gcttacgcat tgatttaagc
     1261 aaaacccaaa acagtgaaat tgcgttaaac aaagaatatg ccgcacaggt ggttcaatca
     1321 ttaaaatggt taaaagaaac cgcaaatgaa ggcgaaatca accttgttga tgtgctacgt
     1381 ttcccgggcg tggtggataa tgaaagccaa gatttagacc aaattgctca agatttatta
     1441 gccggctttg aacaaattct ggcagatttt atcgcaatgc gagccagaga gggcgagaac
     1501 gtgcaagccc tgatcttaca acgtttagaa gcaatttcag ttgaggctgc aaaggtacaa
     1561 gcccaaatgc cggaaattct gcaatggcag aaagatcgtt tacaacaacg gttcgatgaa
     1621 ctgaatttac agcttgatcc acaacgctta gaacaagaaa tggtgttact tgcccaacgt
     1681 gttgatgtgg cggaggaatt agaccgcttg caattacacg ttaaagaaac caccaatatc
     1741 ctgaaaaaag gcggtgcggt tgggcgtaaa ctggatttta tgatgcaaga actaaaccgt
     1801 gaatccaaca cgctcgcctc aaaatcgatt aatgcggacg tgaccaattc ggcggtggag
     1861 ctgaaagtgt tgatcgaaca gatgcgtgaa caaattcaga atttagaata ggacagatta
     1921 tggcaatgaa tttcaagcaa gtgagccaga gctatccaaa tctctgcgtg ccgctttctc
     1981 agtattgttt aattgaaatc gcaggggtag atgctgagaa atatcttcaa gggcaattaa
     2041 cctgcgatgt ggcaaaaatt tcggtgggcg aacatacgct aaccagccat tgtgatccga
     2101 aaggcaaaat gagtgcgtta ctgcgtttgt atcgtgcaga aaatgagaaa tttatggcga
     2161 tcattcaccg cagcttattg cctgaagccc tgacgcaact taaaaaatat gcggtttttt
     2221 caaaagtgac cttcacggaa ttagacaccc cactttatgg tatggcaggt gaagcagcat
     2281 ttgcaaaatt aagcgaaaat atgaccgctt tgcgtttaac cacaggacag cctcgggcca
     2341 ttgtttgggg cgaagagctt gaaacgactg ccgatgaaca gctctggaca ttaatggata
     2401 ttcaagacgg cattcctgtt ctcttgcaac aaaaccagtt tgagctgatt ccacaggcaa
     2461 ccaatttaca ggcacttgaa tctgcgattt cattcaccaa aggttgctac atcgggcaag
     2521 aaacggtagc gagagcgaaa tatcgtgggg cgaataaacg tgctttattt acccttgccg
     2581 gcaactttga aagcgaaatc agcttaccgg aagttgcttc tgcgattgaa atgcaactgg
     2641 gcgaaaattg gcgagcaacc ggcactgtgt tgaccagtgc gactgatgaa aataccctgt
     2701 gggtgcaagt ggtgctgaat aaagatgttg aacctaacac caagtttaga gccaatggtg
     2761 tagcgttgga aatggtcgag ttaccttata gtttggcaga aaatgagtaa aacgaacggt
     2821 catcagggta tgaccgtttt tttatatctg atcagggtga tgaatcatca caaaacgttc
     2881 ccacaactgc tcttcggttt cgatattatc aggatctttg ataatgcagt tggtaatcgg
     2941 gcaaactttc tggcaagtcg gcttttcata atgcccgaca cattcggtac ataacaccgg
     3001 atcgatgata taaatatcat cgcccaccga aatcgcctcg ttcgggcatt ccggcaagca
     3061 catatcacag ttggtacatt tgtgggtaat taataaagcc atagtttata ttgagaaaat
     3121 aaaaattaaa caagcggtcg attttgcaaa aaaatttgca aatcttaccg cttgtcacta
     3181 aatgctacct attataaagc aaaataattt gaactacttc acatttttgc aatttggttg
     3241 tttatccgat taagaaaatt ttttatgatg ccatcgaaac gtttcgatgg agtattttcc
     3301 aacgtgatgg agcgaaaata aaggggttag tatgaaaaaa acgctaaatc taaatgcaca
     3361 actttcgcac agtattgcga cactcgggca taccgatagt ttaacggttt gcgatgccgg
     3421 tttgccgatt ccaaacacaa tagagcggat cgatttggct ttaaccgccg gtgtgccaag
     3481 ttttttacaa acatttgatg tagtagttag tgaattattt gttgaacgag tgctacttgc
     3541 tgaagaaatt aaacataaaa atccgcaagt gttagcagct cttttggagc gtttaaccca
     3601 attagaacaa actcaacaga ataaaattca aattgattat gtgaaccacg aaatctttaa
     3661 agagcagaca cattacagta aagctgttgt gcgttcgggt gagtgttcac cgtatgccaa
     3721 tattatttta tattcgggcg tgccgtttta gggtggaatg atggaaacct tattaaaaat
     3781 gagcgggata gataaagaat ttcccggcgt gaaagccttg agcaatgcgt gcttatctgt
     3841 ttacggtggg cgagtaatgg cattaatggg cgaaaatggg gcaggtaaat ccaccttgat
     3901 gaaagtattg acggggattt atagcaaaga tgccggctct attagctatt taggcaaaga
     3961 agttaccttt aaaggcccga aagattcaca agaagccggt attagcatta ttcaccaaga
     4021 gctgaattta gtcggtaatt taaccattgc tgaaaatatt tttttgggcc gtgagtccaa
     4081 aaccgcttgg ggagctattg attggaaaaa aatgtatgcc gaggcggata aactgcttgc
     4141 ccgacttggg gtaaaacaca gtagccatca gctttgtgcg gagttatcca ttggcgagca
     4201 gcaaatggtg gaaattgcga aagccctgag ttttgagtct caagtaatta ttatggacga
     4261 accgaccgat gctttaaccg atacagaaac agcatcgttg tttaaagtta ttcgagagtt
     4321 aaaagcggaa aatcgaggca ttgtttatat ttcccatcgt ttaaaagaga tttttgaaat
     4381 ttgtgatgat gtgacggtgc taagagacgg gcaatttatc ggcgagcgag taattgccga
     4441 tattgatgaa gatcagttaa ttgaaatgat ggttggtcgc cgtttggaag agcaatatcc
     4501 gcatttacac caagagcgtg gcgagttatt acttgaagtg aagaatttaa gtggaagtgg
     4561 ggtgaataat gtgtcattcc aattacacaa aggtgaaatt gtgggtattt ccggcttaat
     4621 gggggcaggg cgtacagaat taatgaaggt gctttatggt gcattgccga aaacagccgg
     4681 ctcgatagag ttaaatggaa aagcgatttc caaccattgt ccgcaagatg ggttgaataa
     4741 cggcattgtg tatatttcag aagatcgtaa aggcgatggt ctgattttgg gaatgtcggt
     4801 aaaagaaaat atgtctttga cctctctcga tcatttctcc aaagccggca gtattcgcca
     4861 cgatgcagaa aaattggcgg tcgatgattt tattgagatg tttaacatta aaaccccaag
     4921 tcgggagcaa caaatcgggc tgctttccgg tggtaatcag caaaaagtgg cgatcgctaa
     4981 agggttgatg acccgcccga atgtgctgat tttagacgag ccgacacggg gtgttgatgt
     5041 gggggcgaaa aaagagatct atcaattaat taatgaattt aaaaaagatg ggcttagtat
     5101 tttgatggtt tcgtccgata tgccggaagt gttgggaatg agcgaccgca ttttagtgat
     5161 gcacgaaggg caaatcagtg cagaattttc acgagcagaa gcgacacaag aaaagctact
     5221 ggcagcagct attggcaaaa gtacaggatt aaattaaggt gtgtttatgg caactcaaac
     5281 aaagcagttt caacttggca gatttttaat cgagcaacgt tcttttgtag cgttatttat
     5341 tttaattgtc agtgtttcaa tgattaaccc tgattttttc agcgtggata atattttaaa
     5401 tattctacgc caaacctcgg tgaatgcgat tattgcggtc ggaatgacct ttgtgatttt
     5461 aattgcaggc attgatttat cggtcggttc ggttttagcc ttaaccggtg cggttgcagc
     5521 gaccttagtc ggttctgaat tgccgatttt gttggtgatt ccgctggttt tattattagg
     5581 gacggcattt ggtggtatta gtggggcgat tgttgctaaa ggtaaagtgc aggcatttat
     5641 tgcgactttg gtcacaatga ccttattgcg tgggattacg atggtttata ccgatggcag
     5701 accgattagc accggctttt ctgaccaagc agacgccttt gcgtttattg ggacaggcta
     5761 tttatttggt attcctgtgc cgatttggat tatggcggta gtgtttgggt tagcttggta
     5821 tattttgaaa cacaccccga ttgggcgtta catttacgct ttaggtggca acgaatcggc
     5881 aacccagctt tcaggcatca atgtgaataa aattaagatc ttcgtatttg ccgtgagtgg
     5941 ttttttatct gctcttgccg gcttaattgt gacttcacgt ctctcttctg ctcagccaac
     6001 ggcaggtgtg tcctatgagt tagacgcaat tgcagcggta gttgttggtg gaaccagtct
     6061 aatgggcggt aaagggcgtg taatgggaac gcttgtcggg gcattaatta tcggtttctt
     6121 aaacaatgcc cttaatttat tggatatttc ttcatattat cagatgattg ctaaagcgtt
     6181 ggttatttta gcagctgtat tagctgataa ttatctaggc actaaaaaag tttaatctat
     6241 ctttaaagga gatttctatg aaaaaattaa ctacgattgc atcagcggtt atgttaagtt
     6301 tatcggttgc agccactgct tcggcaaaag agactatcgc tcttgcagta tcgactttag
     6361 ataacccatt ctttgtgagc cttaaagacg gtgcacagaa aaaagcagat gaattaggct
     6421 acaaattagt ggtgcttgat tcccaaaatg acccggctaa agagctttcc aatgtggaag
     6481 acttaacggt gcgtggggca aaagtgttat tgattaaccc aaccgattcg gaagcggtcg
     6541 gaaatgcggt ggctattgca aatcgcaaca aaattccggt tattacactt gaccgtggtg
     6601 cagcaaaagg cgaagtggtt agccatatcg cttccgataa cgttgccggc ggcaaaatgg
     6661 cgggagattt cattgctcag aaattaggct caggggcgaa agtgattcaa ttagaaggca
     6721 ttgccggcac ttccgccgct cgtgaacgtg gtgaaggctt taaacaagcg attgaagcac
     6781 ataaattcaa tgtattagcc agtcaaccgg ctgattttga ccgcactaaa ggcttaaatg
     6841 tgactgaaaa cttgctctca agcaaagggg acgtacaagc tatttttgct caaaatgatg
     6901 aaatggcact aggagctttg cgtgcagtga gttcagcgaa gaaagaggtt gtaattgtgg
     6961 gctttgacgg cacagatgat ggcgtaaaag ccgttgaaag cggtaaactt gccgcaacga
     7021 ttgctcaaca acctgaactg attggctcat taggcgtaga aactgccgat aaagtgctaa
     7081 aaggcgaaaa agtggaagca aaaattccgg ttgatttaaa ggttatttca aaataattat
     7141 acttgaagat agcgttcgtc tctgacgaac gctttgaatt aataaaatag cactcgtcag
     7201 agactagtgc tatcagaatc ctagttttgt tgtactaggt aatttgaggg cttaatatga
     7261 aaaaactcac cgttctcggt agcattaacg ctgatcacgt gatttccgtt ccctattttg
     7321 ccaaaccggg ggaaacgctc accggacata gctgccacat tgcttatggc ggtaaagggg
     7381 caaatcaggc ggtggcggca gcacgtttgg gagctaaggt ttctttcatc ggttgtattg
     7441 gcagtgatga catcggcaaa gcaatgaaat cagcctttga gcaagatggc attgacactc
     7501 gctcgattaa aacgattccg aatgaaatga ccggcattgc aatgattcaa gtggcggaat
     7561 cgggcgagaa cagtattgtg atttcagccg gagcgaacgg tcatttggat gaaacggttg
     7621 tggcaaagtg tgcagccgaa atcacacagg cggattattt gctaatgcag ctagaaacac
     7681 cgttgccggc gattgtgcaa gcagcaaaat tggctcagca aaacggcacg aaagtaatat
     7741 taaaccctgc gcctgctcga gcgttacccg atgaactgtt aaacttactg gatatgatta
     7801 ccccaaatga aacggaagcg gaaatattaa ccggtgtgaa agttgttgat gaagccagtg
     7861 cgattcaagc cgcacaagtg tttcaccaaa agggcatcga aaaagtgttg attacacttg
     7921 gctcaaaagg ggtatttgta agcgaaaacg gtgtaggcaa aatcgttgca ggcttccgtg
     7981 ttcaagcggt ggataccact gccgcagggg atacttttaa cggagcatta gtgactgcaa
     8041 tgttagaaga taaaccgctg gatcaagcca ttcgttttgc tcacgcagcg gcagcgatta
     8101 gtgtgactcg caaaggggct caaccttcta ttccgacccg tcaggaaaca ctggattttc
     8161 taagccaaca agcggtgagt ttttaggaaa attttgcaaa tggcaacaat gaaggatatt
     8221 gcacggcttg cacaggtttc cacctcgacc gtttctcacg ttatcaacaa tagccgattt
     8281 gtcagcgagg aaattcggga aagagtgcag agggtggtga aagaactgaa ttacacgcct
     8341 tctgcattgg ctcgtagcct caaagtgaat gaaactaaaa ccatcggaat gctggtaacg
     8401 gcaaccggca atccgttttt tgcggaggtg atggcggggg tggagcaata ttgccagcaa
     8461 catcaatata acctgattat tgccactacc aacggtgaca ccgagcgttt gcaacaccat
     8521 ttgcaaatgc tgatgcaacg aaaagtagat ggcttgttac tgatgtgtgg cgataccaga
     8581 ttgaatgccg atgagagctt gaatattccg ctgccattgg tggtgttaga ctggtggttt
     8641 accgagctca atgcagataa aatctttgaa aattcaatat ccggcggcta tctcgcaaca
     8701 aaaaccttaa ttgaagcagg gcattctaag attgcgatta ttactggaaa cctaaaaaaa
     8761 tcattggcaa aaaatcgttt agaaggctac aaaaaagcac ttgccgaggc gaatattcca
     8821 ctcaatccaa actgggtgtt cgaaagccat tttgattttg ccggcggggt taatggaatg
     8881 aatgctttgc ttgcccaaag cgaaaagccg acagcggtat ttgcctgtag cgacaccatt
     8941 gctctagggg cttaccaagc aatctggcaa caaggtttac gaataccgca agatatttcg
     9001 attatcggct atgacgatat tgaactggca aaataccttt ccccaccgct gagtaccatt
     9061 agccagccca aagcggaact cgggcggctt gcggtggaaa ctttgctaaa acgaattcgt
     9121 tttaaatcca ctgattttca aaccataatg cttgaacctg aattagtttt aaggcattca
     9181 atccaaacaa taaacaaaaa cgctgaataa tgtgaagtta ttcagcgttt tttatccgat
     9241 ttcaattagc ctttttgctg ttccaaataa agcacggtgg cggcaactcg ggaatgcacg
     9301 tttaatttac gcagtaaatt gcggatatgc acttttacgg tttcttcgga aataaagagc
     9361 tgagccgcaa tttgtttgtt cgacataccg gttgcgagcc attgtaaaac atccagttca
     9421 cgatctgtta agcaacttaa aggatcattt tctggctggc ggttgagtaa atgttggcga
     9481 acggtttcgc ttaataccac ttcaccggca gccgctttac ggatattatc tagtagttcc
     9541 ggggtgtcgg tatcttttaa taaatagccg tccacaccag catccattaa tgcaaataca
     9601 tcggattgct cgtctgacac tgttaaaaca atgattctgg catctatacc ttgagcgcgc
     9661 aatgagcgta aagtatctag ccctgaaagc cctttcatat ttaaatccag aataatcaca
     9721 tccggctcaa gtcgtaatgc aagtttgata ccgtcggtgc cgttgcttgc ttcacccact
     9781 acactaaatt ggtgatctaa ttccaatagt tgacgcattc cctgacgcat taatgggtgg
     9841 tcgtcaatga gtaaaatttg agttggagct gtcatttatt tctcctttcc tttctcttaa
     9901 tcggttaatt ttgcaattat taattcaaaa tagcaagttt agggatttac taaatgccgc
     9961 ttttttgatc ttagctaatt tttaactaaa aaagaaattg caaaactttg caagaaaaag
    10021 accgcttgtt cggctataat tactgccaat ttttatttca agtgtgaaaa gaatgacaaa
    10081 tcaaacaacc tattttgcca cggcagcccg tggttttgag gaaatactaa aaaccgaact
    10141 ggagcagatt tgtgcggcag aatgcaaagt cgctcaaggc ggtgtgcatt ttaccaccac
    10201 gcaaaaaggg gcttatcagg cattattaca cagccgttta gcctctcgga tcttactgcc
    10261 gctcatcagc accaaaattt ttagtgatag cgatttatat gcttccatcg tgtcatttaa
    10321 ctgggcggat ttattcgatc cccgtgatac cttttacatt gatttcagcg gcactaaccg
    10381 agaaatccgc aacacccaat ttggggcaat gcgagtgaaa gacggcattg tggattactt
    10441 tgaacgcaaa ggttttgccc gcccaacagt agataaagat aatccggatg ttcgcattca
    10501 tgtttatttg gatcgtgaaa atgttgtggt ttcgcttgat ttaagtggag atgccttaca
    10561 tattcggggc tatcgggaag aaaccggtaa agctccgttg cgtgaaacat tggcggcagc
    10621 cattattttg cgttccggct ggaaagtcgg cacgccattg gttgatccga tgtgtggttc
    10681 aggcacgtta ctgattgaag cagcacaaat ggcagcgaat attgcccctc aacttcaccg
    10741 taaacattgg ggctttaatg cgtggaaagg gcatcaacaa gcgatgtgga aagccgtgtt
    10801 agatgaggca ttccgaaatg ttgaattggg tgcggtaggg gagaattgca attcgtccct
    10861 acaaaaaatg ttcttcggtt ttgatctcga tcatcgtgtg ctgactaaag caaagcaaaa
    10921 tgcgaaaaat gcaggggtag atcatctcat tcaatggcaa caaggtgatg tggctgcgtt
    10981 aaaaaatccg attccggaaa aaatcggcac ggtggtgtgt aacccccctt atggtgaacg
    11041 tttaggcaca acgccggcgt taattgctct ttattcggtg ttcgggcaac gcctaaaaca
    11101 gcaatttgcc ggttggaatg cctcaatttt tagcggcgag cctgaattgt taaattgcct
    11161 gcgtttacgt tcagcccgtc aattcaaagc gaaaaacggt ccgttggatt gtgtgcagaa
    11221 aaattactcc atcagcgagc ataacaagcg gtcagattta gatgaaaatt tacaatttga
    11281 acagaatgct caagtcgctc cggattttgc aaatcgctta accaaaaata tcaaaaagat
    11341 cgaaaaatgg gcgaaacaac aaggcattga tgcctaccgt ttatacgatg ccgatctgcc
    11401 tgaatataat ttagcggtgg acagatatgg cgaccacatt gttgtgcagg aataccaagc
    11461 cccgaaaaat attgatgagc aaaaagcccg ccaacgcttg ttagatgcag tctctgctac
    11521 gctttatgtg acaggtgttg aaactaataa attagtgttg aaagttcgcc aaaaacaaaa
    11581 aggcacgaat cagtacgaaa aacttgccaa taaaggcgag tatttctatg tgaacgagta
    11641 tggggcgaaa ctgtgggtga atttaaccga ttacttggat acgggtttgt tcctagacca
    11701 tcgcttaacc cgtaaaatgg tgggcgaaat ggcaaaaggc aaaacattcc tgaatctgtt
    11761 tgcctacacg ggttcagcta cggttcacgc tgctcttaat ggggcaaaat cgaccactac
    11821 agtggatatg tcgaacacct atttgaactg ggcggagcaa aatttagagc tgaataattt
    11881 atcacttcgc catcatcgtt tgttccaagc cgattgcttg caatggctgg cagaatgccg
    11941 tgagcgattt gagctgattt ttgtcgatcc gccgactttc tccaactcaa aacgaatgga
    12001 aaacagttgg gacgtgcaac gagaccatat tgagttaatg aaacagctta aacgtatttt
    12061 aaccgctgat ggtaccattg tgttctcgaa caataagcgt ggctttaaaa tggattttga
    12121 cggtttggca gcattaggct taacggcgga aaatatttcg cataaaacct taccgttgga
    12181 ttttgaacgc aatccgcaaa tccataattg ttggattgtg aagaatgtat agataatacc
    12241 ctcgaccctt gtgggagagg gatagatttt tcttcgttca gaagaaaaat cagggagagg
    12301 gtaagaagtt actgatgaaa gagttatttc cccctctctc tgaaaaatat ttctgaacga
    12361 aatatttttc ggtctctctc ccacaagggg agagagtaag gctaaaaaat gttagatatt
    12421 gttttatacg aacctgaaat tccgcaaaac agcggaaata ttatccgcct ttgtgccaac
    12481 tgtggctttc gtttgcatat gattgaaccg ttcggctttg gttgggacga taaaaagctc
    12541 cgccgttcgg ggctggatta tcacgaattt gtggatattc aaaaatataa aaattttgaa
    12601 gatttccttg agcgtgctaa gcctaaacgc ttgttcgcct taaccaccag aggggagcag
    12661 aaccacagtg atgtgaaata tgaacttggc gattttttaa tgttcgggcc ggaaagcaga
    12721 ggcattccaa agccattatt agcggaaatg ccgatgtcgc aaaaaatccg cattccgatg
    12781 tgcaaagaca gccgcagtat gaatttatct aactcggtgg cggtggttgt gtatgaggcg
    12841 tggcgacaat tcggctacca aaatgcggta gcggcacaat ctatttaact tgcaagcggt
    12901 gtgattttgg ctattttttg caagtttgtg gtcaaggttg ggggaggctc gaccttgact
    12961 aattaagaac atctattgtg tctattcttc ttcattttta gctggaggta aaaaattctc
    13021 tccggtaaca actgatttac ctgttttatt ctccaattcc tgacgggcat ttttggcaat
    13081 attgccacct tgttttcctg cttgggcatt ttcatcaacc ccaactgcct gcatactttc
    13141 tgcaatttgc cgactggaaa gttcagcaag tgctgtaaat aacaattcag cttcgctcat
    13201 atgatctcgc agattttggc tttttaaacc tttaagcgat ttatgctttt gtacagatag
    13261 tccgctccat tcttgatgaa tcacattggt taaaaatgca aaatcttgtg gctgttcaac
    13321 ccctgacgat ttccaataat cggtcagttt atttcgggtt tcttgtccca tcattctttg
    13381 ctgaatccat ttttcacttc gtcccatctt ctgccaattc tcccttgctc gatctagact
    13441 ttgacttgga tcgtttattt cctgtaagcg ttcataacct acttttgcta accattgttt
    13501 gaagggctct gctttcggac tagggataga ttgaataagt cggaaaagtt gttcggtatc
    13561 tgctacatca gtcaaacgca tttttccgtc agcagagggc attttcaacc cgtgacaatt
    13621 tgtcacggtt tcattgcctt ctgcctttag cctctgtttt agcttacgcc aataagcaga
    13681 gggatctagg ctttcggtta aaatagcaat cacatcaata atagaaaaat accatttatc
    13741 t
//
LOCUS       DANLUA010000050        13699 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLUA010000050 DANLUA010000000
VERSION     DANLUA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..436)
                     /locus_tag="PXB44_001074"
     CDS             complement(<1..436)
                     /locus_tag="PXB44_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5281563.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDN"
     gene            complement(544..1770)
                     /locus_tag="PXB44_001075"
     CDS             complement(544..1770)
                     /locus_tag="PXB44_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5281564.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            complement(1767..2765)
                     /locus_tag="PXB44_001076"
     CDS             complement(1767..2765)
                     /locus_tag="PXB44_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL5281565.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            complement(2776..3777)
                     /gene="pdxA"
                     /locus_tag="PXB44_001077"
     CDS             complement(2776..3777)
                     /gene="pdxA"
                     /locus_tag="PXB44_001077"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL5281566.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            4211..5575
                     /locus_tag="PXB44_001078"
     CDS             4211..5575
                     /locus_tag="PXB44_001078"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL5281567.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            5665..7200
                     /gene="ddc"
                     /locus_tag="PXB44_001079"
     CDS             5665..7200
                     /gene="ddc"
                     /locus_tag="PXB44_001079"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL5281568.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            7303..7665
                     /locus_tag="PXB44_001080"
     CDS             7303..7665
                     /locus_tag="PXB44_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL5281569.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQVL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(7755..8342)
                     /locus_tag="PXB44_001081"
     CDS             complement(7755..8342)
                     /locus_tag="PXB44_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5281570.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            8518..9066
                     /gene="napF"
                     /locus_tag="PXB44_001082"
     CDS             8518..9066
                     /gene="napF"
                     /locus_tag="PXB44_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL5281571.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            9059..9325
                     /locus_tag="PXB44_001083"
     CDS             9059..9325
                     /locus_tag="PXB44_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL5281572.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            9364..11862
                     /gene="napA"
                     /locus_tag="PXB44_001084"
     CDS             9364..11862
                     /gene="napA"
                     /locus_tag="PXB44_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL5281573.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            11951..12856
                     /gene="napG"
                     /locus_tag="PXB44_001085"
     CDS             11951..12856
                     /gene="napG"
                     /locus_tag="PXB44_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL5281574.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            12856..>13699
                     /gene="napH"
                     /locus_tag="PXB44_001086"
     CDS             12856..>13699
                     /gene="napH"
                     /locus_tag="PXB44_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL5281575.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGM"
BASE COUNT         4042 a         2684 c         3029 g         3944 t
ORIGIN      
        1 tattatctac tcgtccccct gtaataatta attctatttg cttatattca gagagtaata
       61 aagcaatatg tagatcagtt gtaatcactc gaagtggcat atgagcgatt tttttcgcaa
      121 tgcatagcgt tgtagtgcca gcatctaata aaatagaatt tcccggtgaa ataagcgaaa
      181 cggcatattc agctatgcgt tctttttcga caatttgctt aaattgcttc tcatttgttg
      241 ttggttgaaa cggtataaat tggtttaagc tgacccctcc gtgtgtgcga atcacgacgc
      301 cttcttcatc taattttatt aaatcacggc ggatagttgc aggagaagcc tcagtcactt
      361 ttaccagttg ctctacggta gcaatattgt ggttttttag gaatagtaaa atttgttcta
      421 gacggttttt agacatataa actctctaat ttttaactgc ttttactata agtaattttt
      481 taaaaataag atagttaaaa ttaaagaacc taaaacattg ttgctttagg ttctttgttg
      541 ttactagttc attatattgc ttaagagtgt gttgtttcca aatcctccag atttagtcat
      601 tacagtgatg cctgagtagt tgcccttttc aatggagcat aaaggaacgc ccggtactac
      661 cgcatattgt acattcatct ctgttacatt catttgatct aataatgctg ctgcaccatc
      721 tccaccaaat aatataaggc tatcaaattt ttcattatca aggcagaatt tacttaaatc
      781 agctaaatgt tgagaaattt ctttagaagt ttccgtgata ttggcagatt ttgcatttac
      841 aacttttgca gggtttgccc gaataataac atgatctcta ctaaaacgta ttagagcgtt
      901 taattgtagt ttcagtgaat gttctgattg cgagtagtta atcaactgtg aaggcgaagg
      961 attaaataca atagattttc ctcctaaagt tgaaatatat tcatcaactt gcagttggct
     1021 tgtttcgtga attgaggtta cgacaattaa tgctctacct aatttaagat ttcgctctgg
     1081 ttgttgttga ttttggatat ttttcaactt ctgtgccaat ccggctgagc caacaggaat
     1141 aattttatta cctatgtgat ttatgacttg ggcaatcatt gagagatctt cgtgcgtttt
     1201 agcatcaata acgatctgtt tatattcact attttcaata gatgcctgca aattatcgac
     1261 cgtatcacac gggattactt gagcatcagg caatagtttt tgcatagaag atgaatgcac
     1321 agggcagata ggatcttttc ctgaagcagt ttcatttaca ggaacaccat taacatataa
     1381 atgaccagct tctatagttc ttcccatttc tggatatgca ggacaaataa ttgctttaac
     1441 atcgggatat aaaccagccc aagctttaat agcaccttca atctgatatt taacagagcc
     1501 tctcattgtg gaatcaattt taaggtaaat ttgcccgata ctattttgaa tagcattgct
     1561 aattgcaatt tgagtcagct cttttgcttt ctctccaatt gctcttgaat ctgtagaaac
     1621 agaaattact tgggctttat caggatgaat ttgggctaat gccgaaactt tagtttttaa
     1681 gacagtgtca aatcctgatt cagcaaacat aaccgcggta tcatttgcac cagtaaggtc
     1741 atcagcaata actaagagtg tctctttcat agattttctc cttttatagt gtcttgttct
     1801 aagccacctt gacgttttaa aacccacgct gcaaataatg gagctaagat tgctgaaact
     1861 aatacagctg cggcaatctg tgcagtcgca gtttgaacaa aaggtgctaa tgtagggtct
     1921 gcttgagcaa caatttcagg tgtaataatg gcatttccag cagtagttcc tgctgcaaac
     1981 ccgattcctg attgattacc acgtcttaaa attgttttgt aaccgataaa tacaagaaaa
     2041 cctgtaaaga gagaagctac gatacctaaa ataattcctg ttgctcctcc agtaataata
     2101 gattgcagat tgattcctgt tcctaatgca aaagcaaaga aaggaatgac tattgcacct
     2161 gttggtttca ttaactctgt ccatttagga tctaagttac caataaccat accaataata
     2221 agaggaataa cagctgcaag cattaatgaa agaggaatat ctgctaatcc tgatacacct
     2281 aaaaatagta aaacaaagaa aggtccatca ttaacagccg aagcgacata agctccttta
     2341 tctctataat caccatattt ccctgtaaac gctaaccaaa taccaccatt tgaatttgca
     2401 acaacagtta gcatagctaa taaagaaacg cccataacac catcattacc tacccataat
     2461 cctaaaatca caactaaaag agcgggaata atagttttaa atagtaatac aacaccggta
     2521 tgggcaagta caggtccaga acttctcacg gtaatttgca tacctgtagc gaaaattaat
     2581 agtgcaataa gtggtcctgc tgatgatttg aataaagccg tcgtaaatga acctaaattt
     2641 aaaaattcag gaaagaatgt accaataata gaaccaagaa ttagcggtat taccattgca
     2701 ccgccaggaa ttttattcat accatcatat atcgggacga tggatttaga tgtttgagta
     2761 gacatattaa tctccttagt gtgtcgcata tttgatattt gccatttttg cacccaaata
     2821 gagtgcttca gtcatactac gactatctgc tgttcctttg cctgctattg ggaaagcagt
     2881 tccgtgatcg acagatgttc gaataaatgg aagaccgaca gtaacgttta cacctgtatc
     2941 aaagcctaat aatttgatag ggatatgccc ttgatcgtga tacatcacaa caacaatatc
     3001 gaattcattt aggttagctg cacggtgaaa taaagtatcc ggagggacag gccctgtaac
     3061 attaaaccct ttggcttgag cctcttgaat tgcgggaaca atttcttttt gatcttcatc
     3121 gccaaacatt ccattttcac ctgaatgagg atttaaacca gcgactgcaa tacgaggttc
     3181 tttaaaccca agaatgcgag cattttcttc tgctaaatca ataactgtag caactcgttc
     3241 tttggtaatt aaatcacagg ctttacgaag ttggacgtga gtagtaacgt gaataacatt
     3301 gagtttttca ctggttagca tcattgagta atctttagta ttactgaggg ttgcaaggat
     3361 ctctgtatgg ccaggaaatg gtttaccacc tgcgtgtagg gcttctttat tcagtggtgc
     3421 tgtaacaata gcgtggattt ttcctcccat tgctaaatta actgcctctt caatatattc
     3481 ataagctgct tttccagcac gcggatcaac ttgagcaaaa ggcaaatctt caggcaggtt
     3541 attcaaatct aaaatatcaa taacgccata ttcggctaca aagttatcta gattattgag
     3601 aattcttaat ttcaattctg aatgaacaat aggtaaggct cgttgtataa tttttcgatc
     3661 accgatcaca acagggtgtg ctaactcata aatccgttga ttagctaatg ctttaataat
     3721 aacttctggt ccgataccgg cggcatctcc catcgtaatt cccaatatag gtttcatatt
     3781 tactccttga ttgaaattaa tcattttgaa cgaagtatag cttatatgaa atcataaaag
     3841 tgtgataaac atcacaaaag tgaacttttt taatcttatt gatttaattt aatcaatttt
     3901 aaggtggtaa attggatagg gtatgaaagt gaaaggcatt tattgctaga taaaagcttc
     3961 ccttttcaat aaaaaagagt atcatacgct ctcttaaacg taaccctaca tttaagtctg
     4021 tttccaacct atcccaattt ctttattcct taggggcata tagggtaaaa acaaaaaagc
     4081 tgaaacgctt tttttaataa gtagcgagtc aaatgcccca tggcatctat tcttttagga
     4141 atagaaagct taggctttcc gcaaggcacc cgacttattt ttttcacctc ttgtagaagg
     4201 tttattttca atgacaatta aaactcctat tcaagccgtt cttgcttcaa accaatattt
     4261 tttagatcgc caagatgcaa tggaatcaaa cgtgcgtagc tacccacgta aattaccttt
     4321 tgcttaccaa aaagcacaag gcgtttgggt aactgatgtt gaaggcaatg aatacctcga
     4381 tttcctagca ggtgcaggaa cgcttgcatt aggtcataac caccctaaat taatccaagc
     4441 agttaaagat gtattagaca gtggtttacc tttacactcg ttagatatta cgactccgtt
     4501 aaaagatgct tttacggaag aattactctc tttctttcca aaagataaat atatcctcca
     4561 atttactggc ccgtctggtg cagatgctaa cgaagcggca attaaattag ccaaaaccta
     4621 cactggtcgt ggcaacgtaa ttgctttctc cggtggtttc cacggaatga cccatggtgc
     4681 gttatcgctc accggtaatt taagtgcgaa aaatgcagta caaggcttaa tgccgggcgt
     4741 acagtttatg ccttatccgc acgaataccg ctgtccgttt ggcattggtg gcgaagccgg
     4801 tgcaaaagca gttgaacatt attttgagaa ctttattgaa gatgttgaaa gtggtgtggt
     4861 aaaaccggca gcggtgatcc ttgaagccat tcaaggtgaa ggcggtgtgg ttccggctcc
     4921 aatcagcttc ttacaaaaag tgcgtgaagt cactcaaaaa cacggcatct taatgattgt
     4981 ggacgaagtt caagctggtt tctgccgttc agggaaaatg tttgcctttg aacacgcagg
     5041 cattgagccg gatatcgtag taatgtcaaa agcgattggt ggtagcttac cgcttgcggt
     5101 attagcgatc aaaaaagagt ttgacgcttg gcaaccggca ggtcataccg gtacattccg
     5161 tggtaaccaa ttagcaatgg caaccggcta tgtatcgctt aaaattatgc gtgaagagaa
     5221 tttagcgaaa aacgcacaag agcgtggcga atacttaact aacgcattac gtgaattaag
     5281 caaagaatta ccttgtattg gcaatgtgcg tggtaaaggc ttaatgatgg gaattgatat
     5341 tgttgatgaa cgtcagccaa aagatgcaac aggtgcatac ccgcgtgatt gcgatttagc
     5401 cgttgcaatt caaaaatact gctttaaaaa caaattgtta ttagagcgtg gcggacgtgg
     5461 cggtaatgtg gtgcgtgttc tttgtgccgt gaatattacc caagcagaat gtgaagagtt
     5521 tattaaacgc tttaaacaat ctgttgctga agcagtgaaa gcggtgcgtg gttaatagtt
     5581 agaatggtag gagcgaaaca tttttcgccc taatgtaaaa aggggcgaat atttattcgc
     5641 ccctacacca ataggataga aataatgtca aacctagaaa aacacagaca atccctgttt
     5701 tgtagcgatc caaaatcgat tgcggattat gaatcggcaa tgaataacgc tgtgaaagcg
     5761 gtttcagact ggctgaaaga agaaaaaatg tacaccggtg gctcaattaa agagttgcgt
     5821 gccaaaatca acggttttaa cccaacccaa gctggtgtgg gcgtgcaaaa atcactggag
     5881 catttggtgg aaattttctt aaagccaagc ctcaaagttc accaccctca ttctttggcc
     5941 cacttacatt gcccgacaat ggtaaccagc caaattgcag aagtgttaat taatgccacc
     6001 aaccaatcaa tggactcttg ggatcaaagc ccggcaggtt cgattatgga agagcattta
     6061 attgactggc tacgtcaaaa agtcggctat ggcaaaggaa cttcaggagt ctttacctca
     6121 ggtggtacgc aatcaaacct gatgggcgta ttattggctc gtgactgggc gatagcgaac
     6181 cattggaaaa aggcagacgg ttcagagtgg tcggtgcaag ccgacggtat tcctgccgat
     6241 gcaatgcaaa aagtgaaagt ggtttgttcc gaaaacgctc acttctcggt gcagaaaaat
     6301 atggcaatga tgggaatggg tttccaatcg gtagtgactg taccgtcaaa tgccaatgca
     6361 caaatggacg ttgaagctct tgcgaaaacg ttggcggatt taaaagccga aggcaaaatt
     6421 gtggcttgta tcgtcgcaac cgcagggacg accgatgccg gtgcgattga tgatttaaaa
     6481 gcgatccgta aattagccga tgaacatcag gcttgggttc acgttgatgc tgcttggggt
     6541 ggtgcattat tactgtcgaa agatttccgc cacttcttag acggcattga attaaccgat
     6601 tccattaccc ttgatttcca caagcatttc ttccaatcaa tttcgtgtgg ggcattcttg
     6661 ctaaaagatg aagcgaatta ccgctttatt gattacaagg cagactacct gaattcagaa
     6721 tatgacgaag aacacggcgt gccgaattta gtgtcaaaat cattgcaaac tacccgccgt
     6781 tttgatgcgt taaaattatg gtttaccgtt gaagccttgg gtgaggagct atacgcttca
     6841 atgatcgacc acggggttta tctttctaaa gaagtggaag cctacattaa tgccaccgat
     6901 ggcttggaaa tgttagtgcc ggcacaattt gcctcagtct tattccgtgt cgtaccacaa
     6961 ggttacccgg cagaatttgt ggatacctta aaccaaaatg tggcagatga gttatttgct
     7021 cgtggcgaag ctaacattgg tgtgaccaaa gtcggtgagc atcaatcact gaaaatgact
     7081 actttaagcc cgattgcaac gcttgaaaac gtaagagcat tattagctca ggtgttggca
     7141 gaagcagatc ggattaaaga ttcgattgta aatggaactt atgttccgcc aattgattaa
     7201 actgcgttaa aaataaaaag gtgttttcga catttatttg tgttttaatg tttgcattcg
     7261 gtagtcttta tcgaatgcaa atttttttaa caaggagttt ttatgaaaaa agtcctgggt
     7321 ttaacattga tggcaatggc gttatcggcg tgtaattcaa ctgcttccaa cggttcttca
     7381 caagcaaata caggtgttgc accggcgaat gtgattagcg tgaaacaggt gttagcggca
     7441 gcagatgaca gctacgtgac ggtagaaggc acactcttaa acaaagtcgg tgacgaaact
     7501 tacttattta aagatgcgac agggcaaatc cgtgttgaga ttgacgatga agtatgggca
     7561 ggtcagcacg ttggtagcga aggcaataaa gtccgtttat acggagagat tgacaaaggt
     7621 ttcaataaaa ccgagttaga tgttaaaaca ctcactgttc tgtagaagat caaaccttaa
     7681 tttggttccc cgccttgttt ggcggggttt ttacgttaat taatcaaacc atccttttgc
     7741 aaaaaaacgc aaaattatac cgcttgttcc ttcaatattg tgctacaaga tgctgtgatt
     7801 tgctgcaaaa tagcggtgta atcagtctct tttaaaccaa tattaccgaa atagtgcatt
     7861 tttacatcac gaatcccaca gaactcaaac agcccttttc ccagcgtatg ttcaaatgct
     7921 gctaaaaagc cttttcgttc atatttttca ttggaattac cgagcgttac aaattgttgc
     7981 atttttttgc cggtcagcaa gccgatactt tcaatttccc cgttacgata ggcaaaatca
     8041 taacttaaca ccctgtctaa atagcctttt aaaattgccg gaaagcccat ccaccaaagc
     8101 gggtaaatta acgtaatcac atcagcttca agccaatatt gatgctcaat tgccacatct
     8161 gcttcatagt gtttactgag tgtgccttga aattccgccc agtctaatat cggcttgaag
     8221 ttgagttggt agagatctcg tacaaccacc ttatttggaa cggaagctgc tatcgttttt
     8281 tccaaaatcg catggttaaa gctggaggga ttcgggtgtg cgtaaataat caggtgattc
     8341 atagcgtttt cctttactgt gtcgaggcag ctactagaag attttagcga aactttcttt
     8401 gatgaaaatc aatattttgg cgaatatacc ggcaaatacc ccgtttgagg aatgaacggt
     8461 tttaagtgaa cattagataa tttagcctat tatagtgatg agtaaaaagg tgttttcttg
     8521 actgattatc ctaatcaatc acttcctcgc cgtagctttt tgcgagggca atttttagac
     8581 tcgttaaaaa gccaaaaagt cagtcagcaa gggtatgagc cgattcgtcc gccttgggct
     8641 aatttagcaa attttttaga aaaatgcacc gcttgcggta actgcattca ggcttgcgaa
     8701 acccagatta ttgtaaaagg tgctgcgggc taccctgaaa ttgattttag ccgtggtgaa
     8761 tgcacttttt gtgaaaaatg tgtgcattct tgcgaggcag aggtattccg agcagtttca
     8821 gaacagcctt ggagccataa aattgagatt caagccggct gtttattaaa gctcggaacg
     8881 gaatgccgaa gctgtggcga tagctgtgag atgagagtga ttcgtttccg cccgagttta
     8941 ggtggcattg ccgaaatggt attaaatttg gaaagctgta atggctgtgg ggcttgtttg
     9001 agcgtttgtc cgacaagtgc gataaaaatt cagccattac aaacacaggc aaacagagat
     9061 gagtgaaaaa ttatccgaac aggaaaactg gtatgtgtgc agtctcgtgg tgcaagcaaa
     9121 accggaaaaa ttggaagaag taaaagccga gattctgaag attcctcata ccgaaattca
     9181 cggtgagaaa acagatgaag gcaaattggt cgtgacgatt gaaagcaatg tgcatttagc
     9241 cctttccgat cgaatcgatc aagtaagaga tattaaaggc gtgattgtgg tgtctttaat
     9301 ttctaattac attgatgaac agtgattaat gattattttt gaagccaaac gtgggacata
     9361 gttatggaac tcaatcgtag agattttatg aaagcgaacg ccgctgtggc agctgctgca
     9421 gccgcaggaa ttatgattcc tgtgaaaaac gttcaggcag atgacaccgg tattcgttgg
     9481 gataaagccc cttgtcgtta ctgtggtaca ggttgtagcg tgctagtcgg tactaaagac
     9541 ggtaaagtag tggcaaccca aggtgacccg gatgcagaag taaaccgtgg tttaaactgt
     9601 attaaaggtt atttcttatc caaaatgatg tacggtgcag accgtatgca agagccaatg
     9661 ttgcgtatga aagacggtaa gttcgacaag aacggcgatt tcacgccggt ttcttgggat
     9721 caagccttta gcattatggc ggagaagatt aaaaccacta ttaaaaatga cggcccgaat
     9781 gctgtaggta tgttctcatc aggtcaaaca accatttttg aaggttatgc gaaagtgaaa
     9841 ctttggaaag gtggtttccg ttcaaatacg attgacccga atgcccgcca ctgtatggcg
     9901 tccgctgcgg ttgcctttat gcgtaccttt ggtatggacg agccgatggg ctgctataac
     9961 gacatcgaaa aaaccgatgc gttcgtgctt tggggttcaa atatggcaga aatgcacccg
    10021 attttatggt ctcgtatttc tgatcgccgt ttatcagaca ataaagtgaa agtggtggta
    10081 atgtccactt acgaacaccg ttcgtttgaa ttagccgata caccgattat cttcaaacca
    10141 cactcagatt tagcaatttt aaactacatt gcgaactaca ttatccaaaa cgataaagta
    10201 aattgggatt ttgttaacaa acacaccaaa tttaaacggg gtgaaaccaa tatcggttac
    10261 ggtttacgtc ctgaacacaa attacaacaa aataccaatg cgaaaactgc cggtaaaatg
    10321 tatgacagcg attttgaaga gtttaagaaa atcgttgccc cttatacatt agaagaagct
    10381 caccgtattt caggcgtgcc gaaagatcag cttgaaacat tagcgaaatt atatgcagat
    10441 cctaaccaaa aattagtgtc gttctggacg atgggcttta accaacatac ccgtggtgtg
    10501 tgggtaaacc atatgatgta caacgtccat ttattaaccg gtaagatcgc attagaaggt
    10561 tgtgggccgt tctcactcac gggtcagccg tccgcttgtg gtactgcccg tgaagtgggt
    10621 acatttattc accgcttacc ggcagatatg gtggtcacaa atccgaaaca ccgtgagatt
    10681 gtagagaatt cttggaaatt accggctgga gcaattcctg atgtaccggg ctttcacgct
    10741 acagctcaaa gccgtgcctt aaaagacggc aaaatgaaat tcttatggca gatgtgtacc
    10801 aacaatatgc agggcggccc gaatatcaat gaagagatct tcccgggctg gcgtaatcct
    10861 gaaaccttta ttgtggtttc agatccgtac ccatctgttt cagcagtggc tgctgactta
    10921 attcttccaa cctgtatgtg ggtggaaaaa gaaggggctt acggtaacgc agaacgccgt
    10981 acccaattct ggcgtcagca agttaaaggg ccggcaaatg cccgttcaga tacttggcag
    11041 ttagtagagt tctctaaata cttcaaagta gaagaagtat ggccggaaga gttattagct
    11101 aaagcaccgg aattgcgtgg taaaactcta tacgaaatcc tttaccgcaa cggcaaagta
    11161 gatagctacc aagtgccaac taacattccg ggctatatga atgacgaagc agatcacttc
    11221 ggttactaca ttcaaaaagg cttatttgaa gagtatgcgg aatttggccg tggtcacggt
    11281 cacgatttag ccgactttga cacctaccac aaagtacgtg gcttacgttg gccggtggta
    11341 aaagacgaga aaaccggcga atacaaagaa actttatggc gttaccgtga aggctatgac
    11401 ccatacgtta aagccggtga agaagtggca ttctatggca atgcggacaa aaaagcagtg
    11461 attttaggtg tgccatacga accacctgcg gaagcaccgg acgaagaata tgacttatgg
    11521 ttctgtaccg gtcgtgtact agaacattgg cacaccggta cgatgacccg ccgtgtgccg
    11581 gagttacata aagcattccc aaccaacttg gtttggatgc acccgaatga tgcgaaaaaa
    11641 cgaggcttac gtcacggtga taaagtgaaa gtgatttcac gccgtggtga atatgtatcg
    11701 aacttagata cccgtggacg taataaatgt ccggaaggtt tgatttactc gactttcttc
    11761 gatgccggtc agttaatcaa caaagtgaca cttgatgcga ctgatccaat ttcatttgaa
    11821 accgacttca aaaaatgtgc ggttaaagtg gtgaaagctt aattgtaggg gcaaattatt
    11881 tgcctcagac aagcggtcaa atttgcaaat ttttttacaa aaacgaccgc ttacctaaga
    11941 ggaacccaca atgaaaatgg atccaaatcg tcgccagttt ttaaaaaatg caacccgtac
    12001 cgcagccggt gtttgcggtg taggaatgat tttagggcta cagcaacaac aaagttttgc
    12061 ccgtcaaggg gtagcactac gcccaccggg tgcattgccg gagcaagatt tcctgagtgc
    12121 ttgcacccgt tgcggacaat gtgtgcaggc ttgtccttac gatatgttgc atttggcatc
    12181 gcttatttca ccagtggaag ccggcacgcc atactttatt gcacgggata aaccttgcga
    12241 aatgtgcttg gatattccct gtatgaatgc ttgcccaacc ggggcattaa gcgaagagct
    12301 aaaagacatt gacgatgcac gaatggggct tgcggtgttg ctcgaccacg aaacttgtct
    12361 taactggcaa ggcttgcgtt gtgacgtgtg ttaccgtgtt tgtccgttaa tcgacaaagc
    12421 gattactctt gagaaagtgc ataacgaccg tacctcgatt cacgccaaac tgattccgac
    12481 cgtgcattct gatgcttgca ccggttgcgg taaatgtgaa caggcttgcg tattggaaga
    12541 ggcagcgatc aaagtgttac cgatggattt agccaaaggc ttgctcggtc gtcactatcg
    12601 tttaggctgg caagagaaac aaaatgccgg taaatcgctg attgaagatg ttcacccaga
    12661 gggtttacgc ccagcttatg atgccagaat gccggaaggc ttacacgagc cggtgtatca
    12721 gcatatggaa gtaaaacctg atgtgaaagt ggctacgcca agtcgggcga cacaaaacta
    12781 tgtgccgaac tctacgacgg tggaagctcc ggagcatttc cctgatttag acttgaatct
    12841 caagggggtg aaataatggc agccaattct ccaaaaaatg cgggcttaga ggctcgtcag
    12901 aaactaggtt ggtggcacgc ttaccgcttt ttgattttaa gacgcttaag ccaactcagc
    12961 attattttga tgtttttaag cggcccactt tggaatgtct ggattttaaa aggaaactac
    13021 agcggtagct tattactaga taccgtgccg atgacagacc cattaatgac cgctgaagtg
    13081 ctggcaaccg gctatcgccc tgaatggacg gtattggttg gggcgttaat tatggtggcg
    13141 ttttacggca ttgtcggcag caagctgttt tgtagttggg tctgcccgat gaatatggta
    13201 acagatactg ctgcttggtt aagacgcaaa ctcggtattc gccaaactgc taaaattcca
    13261 cgcaatttac gctatgtgat tttggcggtg attttagtcg gtagtgcgat ttccggaaca
    13321 ctattgtggg agtggatcaa cccggttgcg gctctcggtc gggtgtttgt attcggttta
    13381 ggggcaacgc tttggctggt tttagtggtg ttcttgtttg acttattggt ggcagaacat
    13441 ggctggtgtg gtcatctttg cccgattggt gcaacgtatg ccttaattgg ggcgaaaagc
    13501 ctgattaaag tcaaagtggt agatcgtaac cgttgcgata attgtatgga ttgttacaac
    13561 gtttgtccgg aacctcaagt gttgcgtgtg ccgctacacg gtaaaccgga ggaaagcacg
    13621 gttatcttat ctaaagattg cattacctgc ggacgttgta ttgacgtttg tgccgaaaat
    13681 gtatttgcct tcggaatgc
//
LOCUS       DANLUA010000051        13689 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLUA010000051 DANLUA010000000
VERSION     DANLUA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            45..998
                     /locus_tag="PXB44_001087"
     CDS             45..998
                     /locus_tag="PXB44_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281576.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            1000..2970
                     /locus_tag="PXB44_001088"
     CDS             1000..2970
                     /locus_tag="PXB44_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL5281577.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            2985..3791
                     /locus_tag="PXB44_001089"
     CDS             2985..3791
                     /locus_tag="PXB44_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5281578.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            3854..5440
                     /locus_tag="PXB44_001090"
     CDS             3854..5440
                     /locus_tag="PXB44_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL5281579.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            5486..6868
                     /gene="nhaC"
                     /locus_tag="PXB44_001091"
     CDS             5486..6868
                     /gene="nhaC"
                     /locus_tag="PXB44_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5281580.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(6911..8107)
                     /gene="ispC"
                     /locus_tag="PXB44_001092"
     CDS             complement(6911..8107)
                     /gene="ispC"
                     /locus_tag="PXB44_001092"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL5281581.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(8226..10424)
                     /gene="relA"
                     /locus_tag="PXB44_001093"
     CDS             complement(8226..10424)
                     /gene="relA"
                     /locus_tag="PXB44_001093"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL5281582.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            10678..11985
                     /locus_tag="PXB44_001094"
     CDS             10678..11985
                     /locus_tag="PXB44_001094"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL5281583.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            12170..>13689
                     /gene="der"
                     /locus_tag="PXB44_001095"
     CDS             12170..>13689
                     /gene="der"
                     /locus_tag="PXB44_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL5281584.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFI"
BASE COUNT         3903 a         2952 c         2819 g         4015 t
ORIGIN      
        1 cttagattta attttggagt gtaactacac tcaaggagaa gaaaatggaa attatactcc
       61 gattattatg gcgtcgccta atggctctgc cggtaatgat cttaggcgta actgccttgg
      121 tatttattgt tttacaattc acccccggag atccggcaac cgttgctctc ggtgaaagcg
      181 ccagcgaagc cgctaaagaa atctatcgtg aacaacatgg gttaaatgat ccattgcttg
      241 tacaatattt tcgcttttta gggaatttat tcgtattaga tttcggtatg acaacccctc
      301 cagaacagcc tattgttgat atgattgcta aagcattccc tttaacatta cagcttacct
      361 ttattggggt cttcttagct gctatcgtct ctttcacatt aggtgtaacg gcagcacttt
      421 accgtgatac ttggattgat caattaatcc gtttaatctc ggttgccgct gttgccacac
      481 cttctttctg gttgggtatt ttattgattc aatacttctc cttaaaatta gattggttgc
      541 cttcaggcgg ttttattcca tttagcgaag atccaaaagg ctatgtaaac tcaatggttc
      601 tcccttcact cgctttagct gttcctgtat gtgcctcatt gatccgtgtc gtgcgtacaa
      661 caatggtaga agaaatggat aaagattatg ttcgaacagc catcggtaat ggtgtacctt
      721 actcaacggt gattcgccac aacgtgcttc gtaatgcctt aattactccg gtaacagtat
      781 taggtttacg ggttggttat ttattaggtg gtgcagttgt gattgaacag atcttcgact
      841 taccgggaat gggtaaatta attttcaacg gtatcgtcaa tcacgacttg aacttagtgc
      901 aaggtgtagt attaactatc gcatttactt tcgtgttggt gaatatcatt gttgatattc
      961 tttacttgct cattaatcca aaaattcgga gtttataata tgtttcgcca aggattagct
     1021 gctcgacttg cttcaggcgg tgcaagattt agagcattat caaccagctc aaaaatcgcc
     1081 ttactcttta ttgtatttat cgcattagtc gctatttttg caccattagt agcgccttat
     1141 gacccactgc aaacgctacg tccagtgcaa gctccaagcg gtgattattt attcggtaca
     1201 gaccgtttag gacgtgatat tttctcgcgt atggtatggg gagcaagaac atctctcttt
     1261 atcggtttag gtgcggtagg cgttgcgatt ctattcggta gtattttagg ggcaactgcg
     1321 gcaaccgccg ataaattcgg taacgaagtg attatgcgtt ttatggatat cttaatggca
     1381 ttcccgggta tcgcattagc tgctgtatta cttgccactt tcggtaactc tgtacctgtg
     1441 attatcgtga ctattgcggt tgtttacact ccccaacttg cccgagtggt gcgtgctaac
     1501 gtagtatctc aatgggaaga agattatgtg cgtgccgagc gtgtaattgg tggtagccgt
     1561 acttatattc tattaaaaca cgttgtgcgt aatactgctg caccggtttt agtattcgca
     1621 acagtgatgg ttgctgatgc catcgtattt gaagcatcac tttcattctt aggtgcaggt
     1681 gtccagcctc cattcccatc ttggggaaat atcttatctg aaggtcgtaa ccttgtatta
     1741 agtggcttct ggtgggctac aactttcgca gggattatga ttttattaac cgtacttgcg
     1801 ttaaatatcc ttgctgaagg cttaaccgat gcgttagtaa atccaaaact aaaacgttca
     1861 actcaaagta aagaagatgt cgcaaaacca ttggcaacag acgttcaaga agcaatgtct
     1921 gaaactcttg cgttaaaacg ttacttactc aaattacacg agaaagaaac gacccgtact
     1981 gaccgtatgc aattaaactc aacggccaaa ccgattttac aagtgaaaaa cttatcaatc
     2041 cgtttcccaa atcgttatgg tgaaactccg cttgttgata atatcagctt tactgttcac
     2101 gaaggcgaaa ccatgggctt agtgggtgaa tctggttgtg gtaaatcaat caccgcattc
     2161 tcaattatgg gtttattacc aaaaactgca aaaatcactg gtgaaatcct ctttaccgac
     2221 cgttcaggta aacaacacaa tcttttagaa tcagccgatt taaatgccct tcgtggtcac
     2281 gaaatctcga tgatttacca agatgcatta agtgcgttaa acccatcaat gcgtattaaa
     2341 gaccaaatgg cacaattaat cagccgtggt ggtaaacaat cagcagaaac attattgcaa
     2401 tgggtaaaac ttgacccgga aaaaacgctc aaccgttatc ctcacgagtt atctggagga
     2461 caacgtcagc gtgtattaat tgcaatggca cttgctcgtg aaccgaaact attgattgct
     2521 gatgaaccaa caacagcatt agacgtaacc gttcaagctg aagtgatcaa attattaaac
     2581 gaattacgtg agaaacttgg atttgcaatg gtattcgtga gccacgactt agctttagtg
     2641 gcacaaatcg ctcaccacat cacggtaatg tatgctggtc aagtggttga aactgcacca
     2701 actacgccat tattggcaaa cccaacacac gaatacacac gtggattatt aggctcggta
     2761 ctttctaccg aattgcgtgc agaacgccta taccaaattc cgggcagtgt gccttcacca
     2821 tttgattttg cgaaaggtga ccgttttgcg agtcgttcat taagaccaga tgcaaaccct
     2881 gaacagcact tgaaattagt gccaaccggt gatcacccac aacacttctg ggcttcacac
     2941 ttagaagatc cacaatctaa agaagcataa aggagagcaa tacgatgagt atgaaattta
     3001 tcaaagaaca gccaattatt gaactgtcta atattgttgt gcaatttgct tctcgtgacg
     3061 gcaccttatt taacccgaaa aaattcacgg cggtaaatga tgtcagccta gcaattcacg
     3121 ccggtgaaac agtcggtctg gttggacaat ctggttgtgg caaatctact ttagccagtg
     3181 tgatgatcgg gcttcaaaaa ccaacctccg gcacagtaaa atttaacggc ttagagatga
     3241 aatatggcag tgcggaagct cgcaaatatt tcggtagcca agtgtcagta attttccaag
     3301 acccggcaac cgccctaaat ccgcgtatgc gtgtgttgga tattttaaaa gatccgatgg
     3361 atattcataa tatcttacaa ccgcaagaac gtgagaagcg ggtttatgat ttgctctctc
     3421 gagtcggtct acctcgttca gcggcgttgg tcgagccaac ccgattatct ggtgggcaaa
     3481 agcaacgtgt agcgattgct cgtgcattgg cattaaatcc gaagctgatt gtggctgatg
     3541 aaccaacctc tgcacttgac gtatcggttc gagctcaagt actgaacctg ctggcagact
     3601 tgaaaaaaga actcaattta gcgatggtat ttatctctca cgacttacaa accgttcacc
     3661 aagtttcaga ccgcattgtg gttatgaacg gcggtaaaat tgtggaaatg ggcgatgctt
     3721 cagacgtatt tgaacgccca acgcaagagt acacccgaac cttaattgaa gcagcaccat
     3781 ctttattata attgtgcttt gtgcctacaa gcggtaaaat ttttcgcaaa ttttaccgct
     3841 tgtgttttta tttatgatcc aaattaccca aaccattaac attgatttat taattgtcgg
     3901 ctctggcatc gctgggcttt ctgctgcagt tgaggcaaaa gaaaaaggca ttaatacctt
     3961 actgattagc aaatctacga ttggctcggg agcgagctac tttccgctta aggcaacact
     4021 tggcattcaa gtaaccggcg atgaagccga taaaattcac tttaaagaag atattgaaaa
     4081 catcggcaaa ggcaaaatca accctaatgt ggtacaagcc tatatcgaag attcaccaca
     4141 agcgattgaa ctactccacc gtatcggctt tcagccttgg aagcgtaacg acaaccgtcc
     4201 ggcttgtttt gccaaatacc ctcgcccaat ttatttaatt aaagattggc gggaatctgc
     4261 cctacgagcg aaacagattt tgcaagagca acacattgct ttctatgaaa atgctaccct
     4321 tttacacatc gtcacagaca ataaccaagt gcaaggtgcg gtattttcgc tcactacaag
     4381 cggtcaaatt tcctatattt tttgcaaaac caagcatctt attttagccg caggcgggat
     4441 tgctgggctg tataaagata atctctaccc ggctgatgtt atcggttcac ttcattatgt
     4501 ggcacaacaa gccggggcaa ccttggttaa tcttgccttt attcagttca ttcctgcctt
     4561 tgtcgagcca aaatacaagg tgctatttgg cgaacataca ttgaaatatg tcacccaaat
     4621 caccgattct aagggtaaca atctatttcc acacttaaat gccgagcaat ttgcaaaaat
     4681 ggtgctggaa cgtagccatt atgctccttt cagccttgat tttgccggcg tggagttcga
     4741 tttagtgatg atgaaacatt tgcttgaaaa ccctcacgaa aaaggcattt accttaaata
     4801 cagccccaaa ctgtatgaag acacggaaga gttttatacc gtctatttga attggctcaa
     4861 aaatgaagtc gggattgatt tagtgcaaga taagattgca atcaagccgt ttgctcatag
     4921 ctgtaacggc ggcattcaaa ttaatcaatg gggcgaaagt gcagtaaaag ggctatttgc
     4981 gattggtgag atttctcact gcattgaagg ggcaaaccgt atgggtggta actcagtcgg
     5041 cggtggctta gtgtttgcca aacgtgcggt atcggaaatt gttcgttcgc tagaacagaa
     5101 tgatttgcaa aaaaacagcg aaatctcacc gcttgcaatg gcagaaaaag cattaaatca
     5161 gcttgctaat tcaactgcta atctagaact aactgccagt gaagtgcttt ctactattcg
     5221 agagaaaatg gcgttatacg ccaatgtgta ccgcactcgg agcaacctca aactactact
     5281 tgaggaatta aaccgtttgg aacaagaatt tgacccaatc tatcacgctc aatatcaagg
     5341 cattgaaatt tacaatgcgt taaaaaccgc tcaattagtg gtcaatcaaa tgctaaatga
     5401 gcagagttta ggcgcgcact acttagatga acaaaattag tctataatag ccaacttatt
     5461 taaaaataat aaattaggag tataaatgaa taactaccta cttaaattaa aaccaatgac
     5521 agcggtcata ctttcgcttt tgacggtctt tatcttaggt tttacaatga ttgggcttgg
     5581 ctgggtgcct caaatttcaa ttctagccgt gattgtaatg ctgctacttt tcggcttaat
     5641 caataaaatt ccgtttaaaa atatgcaaga gggtatggca agcgggctga tgtccagtat
     5701 tggtgcggtt tacctattct tttttattgg attattggtt tcagctttga tgatgtctgg
     5761 tgcgattcca accttaatgt attatggctt aagtgtgatt tcacctgaat tattttacat
     5821 ttctgcattc gcattaagtt ctgtagtagg tattgcgtta ggcagtagtt taaccacaac
     5881 cgccaccatc ggtgttgctt ttatcggtat ggcttctgct ttcggtgcga accctgcgat
     5941 tgtagcaggt gcggtggttt caggggcgtt ttttggcgat aaaacctctc cactttcaga
     6001 cactactacc attgctgctt ccgtagtggg cattgatatg ttcgctcata tccgtaatat
     6061 gctttatacc accatccctg cgtggatttt aacagcatta ttcctttggt ttttaaccga
     6121 acctactgat gcacaaaatt tagcaaatat agagaaccta aaaaccaatt tgcaagaaac
     6181 aggattaata cacggctact ctttgctacc ttttgttacg ttaattttaa tggcaattaa
     6241 gcgtgttaat tcaatttata ttattgtaac tacagtaaca ctctcgctaa ttattaccta
     6301 tatccacagt tcgccaaccc ttgaacagct agggaattat ttctttacca gctataaacc
     6361 tgttgaaggc gtagatttag gcgaagtggc atcattagtc tctcgtggtg gcattaatag
     6421 tatgttcttt accatcacta ttgtattgct tgcgttaagt ttaggtggtc ttttacaaat
     6481 cctcggcatt ttaccggcac tacttaacgg cattagcggc tttttaacca gcacaggacg
     6541 tgttactctt gtagttgcca gcactgcggt gggaattaac gtcttaatcg gtgagcaata
     6601 tttgagctta ttgttatcag gtaatacttt ccgctcaact tatgaacgtt taggcttaga
     6661 agtgaaaaac ttatctcgca ccattgaaga tgcaggcacg gtaatcaacc cattagtccc
     6721 ttggggcgtg tgcggtgtgt ttatcagtag cgtattaaat gtaccggtgt tagattatct
     6781 accatacgca ttcttctgtt atatctgttt aatccttacc ttaatttttg gatttaccgg
     6841 ggtaacaatc agtaaaaaag ctaactaata tgtgaaaaat gcctacaaat ttgtaggcat
     6901 tttcttttca ttagagctga agaacagctt gtttggctaa ctctctggcg agtttatcaa
     6961 tatgcaatac atcagcaatt tcactgactt gaattggagc aatattttcc accaccgttc
     7021 tattcacgtt cacaatatcc gtaaagcgga tttgctcgtt taaaaacgcc tcaacggcaa
     7081 tttcattggc ggcattcatc gctgttgtcg catactgtcc ttctgcaaaa gcctcaatcg
     7141 ctaattttaa gttcgggtaa cgggcaaaat ccggctcgat aaaggttagc tcttttaatt
     7201 taaagaaatc taatggtggc acaccagcat taattcggtt tggatatgcc atcgtgtgag
     7261 caatcggcgt acacatatcc gggttgccca tttgagcaat caccgagcca tcaatataac
     7321 gcaccatcga atgaataatt gattgtgggt gaatgatgat atccatttcc tcggctgttg
     7381 cattaaacaa ccagcgggct tcaatatact ccaagccttt gttcatcatt gtggctgaat
     7441 cgactgaaat tttcttaccc atagaccaat tggggtgagc aactgcttga gccggtgtaa
     7501 tcgcatcaaa ttcatttaat ggcttagtac ggaacggtcc acccgatcct gttaaaataa
     7561 ttttactgac acccagttca gcgagtggac aaaacccgac tttttgctgt gcctccggtg
     7621 gaagcgattg gaaaatcgca ttgtgttcac tatctaccgg taagagcttc gcaccggatt
     7681 tacgagcctc atcaataaaa atttgcccac aggttaccag cgattcttta tttgccaaca
     7741 atacggtttt acccgctttc accgcagaaa gtgtcggtaa taaccctgct gctcctacaa
     7801 tagcagccat taccatatcc acttcggaat gtgcagcaag ctcacaaata gctgcttgac
     7861 cggctaacac ttcagtttta gaccccaaag ttgccaatgc cgtttttaat gcttgtgcgg
     7921 atttttcatc atccattgcc gcaaaagtcg gctgatattg acgacattgc tccagcatta
     7981 attcaacatt ctttccacca actaacgcaa acacctcata ttggtttggg tttgggttat
     8041 gctcaactac cgataaagtg cttttaccga tagaacctgt tgaacctaaa atcactaatt
     8101 ttttcatttt gtttctcttt atctaatgac tgataagggc gaaagatttt tcgcccctac
     8161 gccgtaatta tgcggatact cattcgttgt ttgtaaattg tttagcaaaa acaaccgctt
     8221 gtagattaac tcgaaagccg ttttgcttcg ataacatcat ctaattgagc gatacgagcc
     8281 aataatttat tcaaaatttc aacgttgtta agctgaattt ccatatcaat ggtggcaagg
     8341 ctacgtttca catcggttcg gctggaaacg cctaacacgt ttactttttc atttgccata
     8401 attgcactga catctcgcag taagccgttg cggtcattgg caatcactcg aatggttaag
     8461 cttaaaccag aggtatagcc ttcgccccaa tccgcctcca caactcgttc tgggttgaga
     8521 ctttgtaatt caaacagttg ctcacaatct gcacggtgaa tcgaaatccc tctgccttgt
     8581 gtgatgtaac ccacgatttc atcacccgga atcggctggc agcaacgggc aatggaatgc
     8641 attaagttcc ctacgccctc tacaatcaca tagcctttgc ttggcttcgg ctgattggtg
     8701 tttttattca cctgtttaag taccgcttcg tcttcttgtt cggcggtcgg tttaattaat
     8761 tttgattgca agaagtgcat taactgatga agacgaatat cgccaccgcc aataccggca
     8821 tagagatcat caaactgttt taagttatag cgaggcagag catattcttc aatttgcttg
     8881 agcgtaaaac cgaatttttc catttcggct tcaagggcct ctttgccgat tggaatattt
     8941 ttctctcggt ctaatttttt aaaccacgca ataatttttg agcgagcttt gcctgtattc
     9001 acaaaaccgg tattcggatt gagccaatct cggcttgggt tcggctcttt ttgggtgata
     9061 atttcaacct gatcgcccat ttgcagatga taagtaaacg gcacaatccg ccctgccact
     9121 tttgctccaa tgcaacgatg cccgatttca ctatgaattg cataagcaaa atcaagtggt
     9181 gtagcttttt gcggtaaatc gatcacctcg cctcttgggg taaagacata aacacgatca
     9241 tcaaaaattt gcgaacgcat ttctgccacc atatcgcctg aatcggcaag atcgttttgc
     9301 cactcaagca atttgcgtag ccacacgatt ttttcttcgt agcccgaacg ccctgcggtt
     9361 gccccctctt tatatttcca gtgagcggct acgcctagct ccgcatcatc gtgcatttgc
     9421 tgggtacgaa tttgcacttc aatcgttttt tcaccttcgc ctaatacaac tgtatgaata
     9481 gactgataac cgttaggttt tgggttagca atataatcat cgaaatgttc cggcaaatgg
     9541 gtatatttgg tgtgaataat gcctaatgcc gtgtagcaat cttctaacgt tggcactatt
     9601 acccgcacag cccgaatatc gaaaagctga ttaaatctca accgtttttt ctgcattttc
     9661 ttccaaatgc tgtagatatg tttcgggcgt ccataaatct gcacattctt gatttcagga
     9721 cttaaacaag cggtcagatc tgctacaaaa tttgcaatat aacgctcacg atccacacgg
     9781 cgttcgccca attcaaattt agcaatttga gtgtaagaga cagggtgtaa aatgcggaaa
     9841 cagtaatctt ccagctccca tttgagctgc ccgataccca atcgattggc aagcggtgca
     9901 taaacgtgag aacattcttt agcggcgaga acggtatctt cttcggaatg gtggttacta
     9961 tcacgcaggt aggcaattcg ttcggcaagt ttaatcacca cacagcggaa atcgtccacc
    10021 atcgccaaca acatgcgacg gatattgtcg atttgcagat ccgacacatt attggcacta
    10081 agctggcgga tattctccat ttcgagtacg cctttaacta agtttttgat gtcgttactg
    10141 aaatcttctt ttaattgcac caagtcgatc acattgtgtt ttaccagagg aaaaagcatt
    10201 gcggcaacta agctgtcatc gtccatattc aagccgtgca atacttcaac catctcaatg
    10261 ccataccaca tcagactata gtcggtatcc agtttttcct gtgcataact ccacgcattg
    10321 tgaatctgct caaaagtcac aggagacata ttcaggcttg cacaccaact cgacaactca
    10381 aaattattcg gttcaagttg atgggaataa cgaattgcta ccataaggcc tccttgtaaa
    10441 tttttgtgga aattagaccg cttgtacagc ttaaaatgga gttttattat acgtggaaat
    10501 tcagaaaaaa tttaccccta atcgaaaatt taatccaagt tatcttatcc tctttactcc
    10561 ttaagaggta gcaaccttgt gataaatcaa acctatcaac acaatcattt gcacgacttc
    10621 acgctcttga ttaaaatact tgcgttttta ttttgatctt tatttttgtg ggtagcaatg
    10681 acaattttag ctttaggaat caaccataaa accgcctctg tgggtttaag agagaaagtg
    10741 gcgtttgtag atgacaaacg taaactggct cttgaacaaa tccaaacgag cggattggct
    10801 gaaagcgtgg tcattctctc tacctgcaac cgcaccgagc tctacttcca ccagcctaat
    10861 atttctcccc gtgaagagag cgaggaaaat atccaatggc gtgaacaatg tttccgctgg
    10921 tttgcccaaa ttcatcaact tgatgaaagc gagttgcgtg aatgtttata ttttaaacaa
    10981 aatttagaag ccgctaacca cttaatgaaa gtggcaagcg gattagattc actaatttta
    11041 ggcgaaccac aaattttagg gcaggttaaa caagcctatc aatatagcga agatttctac
    11101 caacatcagc aaaaagcgat gtccaccaag ctatcacgct tgttccaacg cacctttgcc
    11161 accgcaaaac gagtacgttc cgaaaccgaa atcggcagcc aagcggtgtc agttgcttat
    11221 gcagcttgcg gtttagcccg tcaaattttc gataattttg gcaagttacg ctttttgctg
    11281 gtaggggcag gcgaaaccat tgaattagtc gcccgctatt taattcaaca cggggcgaaa
    11341 aatataatgt tggcaaaccg cacccatatt cgggcggaaa tgttggcgga aaaactaaac
    11401 gctccaatgc aaattctgtc gttaagtgct ttgcaactcg gtttaaacca agctgatgta
    11461 gttattagct ccaccggtag ccctgatacc ctaatcagta aagaaatggt ggaaattgcg
    11521 caaaaacaac gccaatttga cccacttttg ctgattgata ttgccgttcc tcgtgatatt
    11581 gacgaaaaag ccggcgactt agagggcgtt tacgcttata gtgttgatga tttacaaaat
    11641 attatccaac gcaatatggc tcagcgtgaa gaagccgcca aacaagccgc tgaaatcgtc
    11701 aaagaggaaa gcaaagcatt ttttgaatgg ttaaaacaac agcagtctag tgatttaatc
    11761 aaacgctatc gtcaagatgc ggagcaaacc cgccaagaat tgttggagaa agccctacaa
    11821 gcaatcagtc aagggcaaga cagccaaaaa gtattaagtg agctaagcta caaactcacc
    11881 aaccagttac tacacgcccc aacccaagcg ttacaagcaa tggcaaaaag tggcaactcc
    11941 accggcttac aaagtttttc aagagcatta cggctagaag aatgatgctc agcacattct
    12001 tttaggcata tttgcaaaaa attgaacatt tttaaccgct tgcggacgca cgccgtgcgt
    12061 ccctccccgt gttcccgatc ttcttctaat ttcaacttcc ccaaatttcc gctataataa
    12121 gccaattttt gcaactattt tgcaaataac cggatataga ggatacaaaa tgactcctgt
    12181 tgttgcccta gtgggcagac ctaatgtcgg caaatcgact ttatttaacc gtttaacccg
    12241 tactcgagat gccttagtag cagatttccc cggcttaacc cgagaccgta aatatggtca
    12301 cgctaatatt gccggctacg atttcattgt aatcgacacc ggtggtattg atggtacaga
    12361 ggaaggcgtg gaagaaaaaa tggcggaaca atctttattg gcgattgaag aagccgatgt
    12421 ggtgcttttc ttggttgatg cccgagccgg attattacct gccgatgtcg gcattgctca
    12481 atatttacgc caacgtgaaa aaaccacggt ggtggttgcc aataaaaccg atggtattga
    12541 tgccgactct cactgtgccg aattctatca attaggctta ggcgaagtag aacaaattgc
    12601 tgcagcacaa gggcgtgggg ttacgcagct tatcgagcaa gtgttagctc ctttaggcga
    12661 acagctaaat gccgagcaat ttaaccaaga acaagcgggc gaaaatgcta aaaatgttgc
    12721 aaatgaagaa acagacgaat gggataccga attcgacttt gagaacgaag aagatgcggc
    12781 attattagat gaagccttgg cagaagaagc agaagagtcg attgaagata aaaatatcaa
    12841 aattgcgatt gtcggtcgtc cgaatgttgg taaatcaaca ctgaccaacc gcattttagg
    12901 cgaagagcgt gtggtagttt atgatatgcc aggaacaacc cgtgattcta tttatattcc
    12961 aatggagcgt gacggtcagc aatataccat cattgatacc gccggtgtgc gtaaacgtgg
    13021 caaagtgaat ttagcggttg agaaattctc ggtaattaaa accttgcaag ccattcaaga
    13081 tgcaaacgtg gtgctattaa ctattgaggc ccgagaaggt atttccgatc aggatttatc
    13141 actactcggc tttattctaa atgctggtcg ttcattggtg attgtggtaa acaaatggga
    13201 cggcttgtcg caagatatta aagatcaagt gaaatcagag ttagatcgcc gtttagattt
    13261 tattgatttt gccagagtgc attttatctc ggcattgcac ggctcaggcg tggggaattt
    13321 gtttgattca gtaaaagaag cctacgcttg tgcaacgcaa aaaacctcga cctcaatgct
    13381 gactcgcatt ttgcgtatgg ctgctgatga acaccaacca ccattggtta atggtcgccg
    13441 agtgaaatta aaatatgctc acccgggtgg ctacaaccca ccaattattg tgattcacgg
    13501 caatcaggtt gaaaaattag cggattctta caaacgttat ttaagtaatt atttccgtaa
    13561 aagtttaaaa attatcggct cgccaattcg gattttattc caagaaggca ataatccttt
    13621 tgccggtaag aaaaacaaac tgacaccaag ccaattacgc aaacgtaaac gtttgatgaa
    13681 gtttattaa
//
LOCUS       DANLUA010000052        13661 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLUA010000052 DANLUA010000000
VERSION     DANLUA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            22..1467
                     /locus_tag="PXB44_001096"
     CDS             22..1467
                     /locus_tag="PXB44_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281585.1"
                     /translation="MRSTFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDG
                     NDKEKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKID
                     NSSVQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEK
                     SKKENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSF
                     TDESVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDAN
                     LEGNTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRENGDSYAEYGVFTQSGK
                     EAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNESKLNG
                     KIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQLEEAV
                     GVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            1531..2943
                     /locus_tag="PXB44_001097"
     CDS             1531..2943
                     /locus_tag="PXB44_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5281586.1"
                     /translation="MKKIALLALFPMMLFARDPNTTQAYIEHTAQPTTTPQRVKIEKS
                     VPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYRDFTHIDPVLLRYAQGKIA
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(3193..3516)
                     /locus_tag="PXB44_001098"
     CDS             complement(3193..3516)
                     /locus_tag="PXB44_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL5281587.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(3485..4012)
                     /locus_tag="PXB44_001099"
     CDS             complement(3485..4012)
                     /locus_tag="PXB44_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5281588.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
     gene            complement(4014..13658)
                     /locus_tag="PXB44_001100"
     CDS             complement(4014..13658)
                     /locus_tag="PXB44_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL5281589.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYVLARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLENISENRSHKNEMPGKDNIKSIIDDMKKFQQIGK"
BASE COUNT         3412 a         3274 c         2704 g         4271 t
ORIGIN      
        1 ttaatctgac ggagacaaaa tatgcgttct acattcaaac ttactcttgc agctttagct
       61 gtttctattg ttttaagtgg ctgtaaatct agccaaggtc catctattgt tgcagacagc
      121 cctccaccta aggaaataac agctactgat ggcaacgata aagagaaaac cgataacaaa
      181 gagaaaaagt ctgcgcaacc aaataaatcc gatcctcaac cggataaacc tgctaacgca
      241 aaagacccag taaaacctga tgcggagaca aataaaccaa gtaaaaaaga taaagaatat
      301 acaccacctg ctactgaaaa aatcgataac tcatcagtac agccggataa aatggtgcct
      361 aatcctatcg agaataagga aaacgacagt aataaaccta accccaaaga aagcgatagc
      421 gataataatg acaaagtcac tgaaggcaat aaaccaaatg ccccaacaga aacagtaaat
      481 aaagataaag aagctgagaa aagtaaaaag gagaataaag aggactcaag caataatact
      541 cctaacatta attttgacgg ggcaaagcct atagatacag atagtaaagg tagcccgtac
      601 cgagtaataa acctaaatcc gagaaaaagc gtagtaaatg caagtgctat gcaaaaagta
      661 gatttcgact cttttacgga tgaatctgtt gaaaattcac catttgtcag tagcgatact
      721 gaaaaagaat acaataatac acttgttttt gggcttggtg ccggagataa agattcttat
      781 tttaagaact taaatcaaaa aggggataaa catttaggta ttcatagtgg cgtgtatgaa
      841 gatgcgaatt tagaaggtaa tactaaaaaa attaactatc tctatgtaaa tcaaccttat
      901 tcgagctatg gtgctttatt tacggaccaa aaagacagcc atttattcaa cgttcgtctg
      961 accgctggta gagaaaatgg agatagttat gccgaatatg gtgtattcac acaatcagga
     1021 aaagaagcta aatggaatga taacctgatt ggtgatgcca cttacaaagg tcaagctatt
     1081 gctcgcgtta ttcgtcaggt aaatggggaa caagtcgcag gtattcctca atttgatggt
     1141 gatgttacat taaatttaca cctttccaaa gaatggaatg aaagtaaact aaacggcaaa
     1201 attgatagcc aaagtgttgg ggtaattaca cttaaagaaa gtgaaatagc taaccctacc
     1261 gcactccgct caaatattgg ttttggagaa gaagtcatag ttgagaataa agagaatctt
     1321 gtgggtgatt acgctgtcga ttttaccggg cctcagcttg aagaggcggt aggcgttgtt
     1381 gaattagaaa acgaagccga gacattctct gaaaatgaag tcattaaata caatgcggta
     1441 tttggcgtaa gcaaagcggc gaaatagtat tttttgtatc ttacaaaggg ctgttaagcc
     1501 cttttatttt tattatttta aggataaaaa atgaaaaaaa ttgccttact ggcacttttc
     1561 ccaatgatgt tatttgcccg agatcctaat actacacagg cttatattga gcatactgca
     1621 cagccaacta caacccccca aagggtcaag atcgaaaaat cagtgcctat ttttaacgaa
     1681 actgaattac gtgctaatca attattgacc gaaaagctac ttatccaagc tatttatagt
     1741 cgtaggcttg ctaacatgga aaaattaact gcgatttatc gggattttac ccacattgac
     1801 ccggttttac tgcgttatgc acaaggtaaa attgccctac tgaaaggcga ttacgcacag
     1861 gcaattcagc acttccaaac cattcttgcc aaacaaccca aactaaacac tgtaagaatt
     1921 gagctggcga ttgctctatt gtacgatcag caacttaatc aagcccaagc acaatttgaa
     1981 aaagccaaaa gtgccgaaca tctaccgcct aaagttcacg cattaattga gcaatatcag
     2041 caacatctgg caaaaaaaca ggaatggaaa tttagttttt cagccaatta tgtgcgagac
     2101 ggcaacatta accgaacctc gaaacagccg aacattgagc aaaccggcta tgtaaaacaa
     2161 cctaatttgt tgccccaaac cgcacatggt gttcactatg cgtttggatt acagagagat
     2221 ttcaacctgt tcgactcaca ctatgtgagt tttgacaacc aaacccaagg tgaactttat
     2281 tgggataacc acgattatga tgaaattacc aatcgcacct tgtttggata cgcttacaaa
     2341 aaatccgacc aaaccacccg cttgttaccg ttttatgaaa aacgctggaa acatcaccgt
     2401 tcggaacgct ggacaaacgg aattcgactt gaacacgcac aatggttttc accgcattgg
     2461 caactcgcca ccgcattgga atacggcaaa aaacgttatt ttgatttgcc gatttataac
     2521 ggcagctaca aacttgcctc tgccacattg ctttggttac gcaatccaca acaatttttc
     2581 tttgtcggca ctgatttcaa tcaagacaaa actcagttaa aacaatatag ctctgatact
     2641 aaaggcatac gctttggctg gcaacgagaa tggcaagccc tcgagctttc cagtcgcctc
     2701 acgcttggct atactgacag acgttacaaa gacaccgcta tattgggagg aaaacttaat
     2761 cttggcagaa tccgccatga ccgtgtgcaa agtgctaacc tcacgctttg gaaacgagat
     2821 tggcactggc tcggcctgat gccgaagctg caatttagtt acgccaaaca ccgtagcaac
     2881 ctgccgagcc tctatgccta caccgataaa aacatcaatt taattattga aagtcggttc
     2941 tagctccata aaaacaacaa gcggttaaaa ttcctagaaa ttttgcaaat tgcaaaaaat
     3001 gtagaaaatt taaccgcttg tatctatact gcagcttctt ttccttcagt ataaattaat
     3061 atacaccgtt gcaacaataa tggctgctcc ctgtcatcac cttgaactga aaattgctca
     3121 aggtggatat gactatcgca gccgcttcca ccgcacccag taatgtatgg gcaagcaggt
     3181 tggcttgggt atctactttt taatttgttc gttgagatat ggcgaagcta agcctactgc
     3241 tgcttgtcct gaatctttcg ccgctaaacc ttgtaaggct gccgtcaccg catccaccgc
     3301 acgcttggtt ttgctgcccg tgccgtaggc gttatcaaaa gtctgctgtt tatcgtttaa
     3361 atcccgtttt aattggagat attcaggatg ttgagcgatt tcttcttttg tttttccctc
     3421 atttctcaaa cgggtttcca ctttgcccaa ttccagcttc gcttccgtca ctttttcacg
     3481 ctcactatgc gtgtaaatct gataagcctt ctgcaatctc gcctatgccc ttcaccagct
     3541 cctgtgtttc tttcacctca ctaatatcgt gttctattaa ctctatctct ttaggaactg
     3601 ctccaatcat agacaatgga ataacttgat ctttattttc aatattcgga tctgcccccg
     3661 catttaataa tgctatcgct gcatcaacat tctttgccct cattgcataa tgtaaagggg
     3721 tcattccgta gcaatcttgg gcattaacat ctaccccatg tttaatgtaa aattcgacta
     3781 cagaaagagg agatggtgaa taactattta agttacacat atgtagccag ttccattttt
     3841 cttttggtgt gctatccgta atagtcagtt caccttttac gaaaaatgtt tcttgtaaaa
     3901 gatcgagatc gccttctgag tttgctgcaa taactcttaa ttcaggatct tgatcatata
     3961 attcttgata acgttctaat gtctgctgac gttctttatt cttatctctc attttacttt
     4021 cctatttgtt ggaatttttt catgtcatcg attattgatt taatattatc tttccccggc
     4081 atctcattct tatgactccg attttcagat atattttcta atctgaaatt ctccggtctc
     4141 gcattaacat aatcattaaa ttgtttttga ctccaattta attcttttgc cgctaatgat
     4201 aatcggcgat gttcccagcc atacatatga cctatatcaa taggctgttc aatttcttga
     4261 cgggttaatt tattgcgata attaccattt ggcaattttt cataattagc ataaattttc
     4321 tgtaacgttt ccgagcgtaa ataagctcgg ttatatagcc catctaccgg attatctgtc
     4381 gataaatagc gtcccgtttc ggcatcaatt tgctgagaca aacggttaaa atgtggatct
     4441 accccggctt gatggtttgg cgtttcaaaa cgattgagat ctttttgaac tactgtcacc
     4501 actttcccgt ctttcgggtc tgtgaactga taagctttat agccatcaat attttttacc
     4561 tgtttggcat cattcggtaa agcgtgccaa caatcattat gcacccatac cccttcatta
     4621 tcggcatttg tacctctaat aaagtaagta tgatctttct ctaccgtcat attgtaggct
     4681 tttaatcttt cctgtttgat ttttacctta gtgacagtct gccactcacc actttcagca
     4741 agtaagcgat agcctttttg caaatgttga gcatcaaccc actgagcttt tgctatctct
     4801 ccgttgtaag aatgcccctc cgaagaaatc ggcactctgc ccgataatac ttgcgtaaag
     4861 aacgggtgga ttttgtttga cacaatggtg tggtattttc ctttgctgtc tgcgacttct
     4921 acatacaccg tataatcgta tggattactg taatgagctt ggactttctg ataggtggtt
     4981 ataccggtgc gttcattttt cgccagcacc ttatcgccca ctttaatgct ggaaatctgt
     5041 cgataacctt tatcagtttt gacttccata tcaccgtgga atgagcaagt gcctaaacta
     5101 gcgctcgatc tggtcttagg aatcgcaagt tctttacctg atttggtgac tattttcgcc
     5161 gttgtcccta cacctgtttt aacaagagca ccagcaccgg ttaatgctgt caccacatca
     5221 gtcatcatat tgccaaactc ttttccggca ttaaaagaac ctacagcccc tgcctgttca
     5281 tagtaaacct gcattttatc gagtttagct aaatactcct gtttagtcat attagcaata
     5341 taggagagcg tatcttctct tgcgatcaat tctttcatcg ccacaatagt ttctattggg
     5401 cttgagaaca tggagatcaa tccttcaacg ctctcagcga gattctcagg aacaccagca
     5461 acggcgcctg atacaaaact aacatcttgc ccaatatcaa ctaatcccca atataatcga
     5521 acttggtttt cacagcgggc atcaccatgg caggatttta attcttgtga gcgtttctgc
     5581 tcctgccagt tcgagaggta gttattctcc actgcccttt tcccaatatc cgccccaacc
     5641 gctgccgatt gtgtactatc ccctatcaaa cctgaagcca gtccgcttgc aagctggctt
     5701 agcaacgcca cattctgttt ctcggcttcg gttaagtctt gcgtggattt gcccttgtag
     5761 agggttttaa ctataaagtc cgctgccgct tcgctccctg cgcctgcagc agcccctgcc
     5821 agtacattgt tccctgtcgc agccgcttcc accgcaccca gtaaggcgtg ggcaatcagg
     5881 ttggcttggg tatccccttc ggtgtacttt ttgatttctg catttaagta tggtgaggct
     5941 aaacccactg ccgcttgtcc cacgtctttc gccgctaaac cttgcaaggc tgccgtcacc
     6001 gcttccaccg ctcgtttggt tttactgccc gtgccgtagt ttttatcaaa ctctttttgc
     6061 aagcggtcta aattggcttc taatttacca atctcttcct tgctcgcttt ttgagcttgt
     6121 gctttgccca attccagctt cgcttccgtc actttttcac gctcactatg cgtgtaaatc
     6181 tgataagcct tttctgcaat ctcgcctatg cctttcacca gctcttgtgt ttctttcacc
     6241 tcactgatat cgtatttcgt cactttttga ttggcgtttt gcgtatctcg gttgagatga
     6301 ttaagcgtag tttccgcatt taggtcttcg gtgagggtga tagcgatatt ttctccaatc
     6361 gctgcctttg ttgtgcttcg ctcgctttcg tttttattgc ccagtaagct cagtgccgac
     6421 atcgggttca ccccacttgt ccctccgcta atcgcgacac ttcgggtttt caattcactg
     6481 tggttttcaa tatcgccggt ggtcagcgtg ccggcttgga agcggttgtt ttctttctcc
     6541 gcttggctag tcattaccgc tccctcaaaa tgggcgttct ttttcacttt aacgtccatt
     6601 ccactcttgc ctatgtcgat tgcagtttgg ttttccacct gagcagtgtt caacttcgct
     6661 ccattatatg ccacatgacc atttactcct gttgtcccta ccgccaatgt gccatgtacg
     6721 ctgccttctg tgtattcgct tttatatttg cttatatctt gtcgacttct tgcctcaaaa
     6781 tcttgtacct caccttccca atgatttgct ttgacattgg ttgcatccag catgagttta
     6841 ccatttgcag aatttgtaac aattttaccc gcttgcagtt ggttgttctg ccacgtttca
     6901 gtttcgatat tctccttgcc tttacctcca ctcactcctg cctcaaaacc aataccaaaa
     6961 ctatctctat ctaaaccgat aaatacgccc acactcgcac tgtgattctt attcttagtg
     7021 ttgttttgat aagtatccgc aacacctttg gattcaatac ccagcttacc gtcaagcagc
     7081 agggtgtctt tcactcgggt atccacccct tccagcgcaa ctttgcccgc tcccgaaatc
     7141 aaggcaatat tgccgccgtt cagggtgctt ttgctgtggg tcacgctttg actacggctt
     7201 tctctttgct gtttttgcgt gccgagactg actgaaatct tcaccgccgg attttctgcc
     7261 gcagcatggt tttctgtgag tgcattgcca agattgccca gcgtttctgc cactttctga
     7321 gcattttgtg ctgcaatcac ggcatcctcc gccgctttta ccgcatatac ccctttgagt
     7381 ttctcgtttt gtgttgcctt cgctttttgc accgattgcc gtgccgattg caccgcatcc
     7441 gttaccggtg tactcaacgc aatgcttaag cccgactggc taaattcata attatcggtg
     7501 ctgttaataa catctttccc tgattcaacc cgtaccccgt ttttgcctga aatcacggcg
     7561 gttttttcgt tggaaatcag cacatcgaca ttgctcacgc tcgctttatt gccggcttga
     7621 atccgaatat tgcccccttc actgcccaat gtattgcgtg ccgtgctttg gattgccccc
     7681 tctaagctgt tctcttggcg tgttttttgg ctaccgatag taaagccgat ttcaccaccg
     7741 ttcaagaaac cgctgcgttt tgtctcacga atatgctgtt ctgaaagttg gtttgttgcc
     7801 gaactgagcg ttacattatt accgccgata atatcaatat cgccactgcg tgagacggca
     7861 agaagacttt caccttgaat atcgtgaccg gctttcacta aaatgtcttc accggttagt
     7921 tcagtgcctt tgtgttcggt ttcagaaata ttgtcataag tcgtttttgt tttcttcgct
     7981 agcgcaccac cactttttgt tttgtgatac tcttcataat cgcgagaacg tgttgcgcta
     8041 cccaacacca tatcattttc agccagtaac gctaaacgcg ctttggcatc caaatccgct
     8101 ccttcactgt aaaggttttt ccctgaaacc accacatcgc ctttgccttg cacccgtgaa
     8161 atgaccacat cattcaaggc ttcattacga tagtgattgc caccgcccat tttttcatca
     8221 aaaccaacct gtaaagcggt caaattcaac tgattttttg cagataagta ggtctgcccc
     8281 tgtccgtcat tgatgatgtc tgcgcctgct gaggtgatgt tgcctgcctg tacttgcagc
     8341 agtccatttt catctttaac gtgcagtagg gctttgcggt caaggttggt ttcagcgcgt
     8401 ttgaaaccgt ctaggtcaat ctgtgtggtt cgagtggtgg aggtatggtt aaaattgccc
     8461 gcaatatcca gcaaaatcgc tttatccgct tcaatcttgc cgccgatatt ctctagatca
     8521 ccgctcactt tggcagcaag cacgccacca ctgagtgtgc cactattctc taaccgttcg
     8581 gcattaaact gtgctaaacg gcggcctgct atcgtgcctt ggtttacaat ctctttactg
     8641 ttgatggtaa gcgcatcggc tgaaatcagg ctgcctttgc cgtcaatatc acctttgcgt
     8701 gcaaggacat acactttcgg cactaatacc gataccgttt caccgcttgg caagcgaacc
     8761 gtttgggttt caaaccaaac aatatcgctg gtcaagcttg ccacttgctc cggcgataag
     8821 gtaatgccta accgaagatt gaatttctgc gcaaaggtga tgccggcatc catcagggct
     8881 ttatattggc tatcaaaatc cgtatagtcg cccaaaaatt gtctgccggt taagcggttg
     8941 atttgctctc gaattaagcg ctgttcataa aaaccatcgc ccaaacgttt atgcacattg
     9001 ttatggtcgt ggcgtagggc attgaacata taatcactgc ttaaccagcg ttttttatcg
     9061 gtgaaatccg gatcggtttc gatcagaaca tggctatccg cttgagggtt aatgcgataa
     9121 agcccttggt ttggcaaacg ggtatcgacc gtcattgagc gaacctcaaa tgcggctgca
     9181 ttcgggcttg gtatgcgttc aatcgccatt gttggcggat taggggcatc gggtagatta
     9241 ccccgttcaa cctgattgcc ttccggcttc acggcatttg ctgctttagc gtccacataa
     9301 tctgtataag cggtcggtga agtattttcc agctcgacaa aaatattcat atccttatgg
     9361 acttctttta aacgagcgag aggaccatca ttttcatcgt agcgggagtg gcgacgtctt
     9421 gtgcctccgt ggtactcagt aaaagagaat tggcgccttc ccagttccca atcttggcga
     9481 acagcatctg catcatcaag gttggcaatt ctactgcgac caatcgggtt taacgctttg
     9541 cctgcaataa cccaactttt atcgttgata aattcatcgc tttgataagt gagatcgcca
     9601 cctgccaaga tctgtgccgg taagctggct ttggtgatat tctcttttac catacgctct
     9661 ttaatccaca aattccagaa atgcgggtat tcttggctta aattcgcttt attgtgcttt
     9721 gcaatcgctt catctaatgc ttgaatattg gcaccatatt ttgcttccca atcaattaac
     9781 ggttttaata gcacataacg ctgataagcc gcttctgccg cttcataggc tttcagttcc
     9841 gcttgatatg tttgtagtcg tttttggtat tgggcttccg tttccgtaga gcctctaaca
     9901 ggttcaatcg gtcttgccgg aaccaccggt gcaatgcctt gcccttgttc cttctgtaat
     9961 ttttcctgct ctgcctcact taaatgctga ttcgctaaat cttcttcatt cattgcttga
    10021 ataatttcag gcgtcatttc cggtgcatct tcagctggtg gtgttaaatt aaaccacgtc
    10081 catactgggt tatctttttt ataattacct gcaggtaata gagtacaatc tacactttca
    10141 tcattcgcac agctttgagc atagggtttt gccaatacgg tcgtggcatt cagctggcta
    10201 atatcggtaa ttaccggcaa gtttgcttca tcatactttt gccaatagct ccacgttttg
    10261 cgatagtagc ctttgcggag ttgattcaca ttaattgtcg gcaaatgccc gttatcatat
    10321 acatcacttc cacctcgatt atgcttatct cttggcaaaa taaaatgttc gttcacagtt
    10381 tggcgatcag tggtctccaa ttccgaacga taatgactat ttacattcgc taccttatcc
    10441 gcattcaact gaatacgccc ttcagcttca ataatactgc tgcggttatg tagctgccct
    10501 gcgttgccaa taacggtatc ttgctcattt aattgacgtc caatcgctat ctctcctccg
    10561 ctatatatta atgaaccgct tttcttattc gcttcataaa ctagggtatt attttcaatt
    10621 actttagccg ctaaaccgag agattgacga gcagcaatag tggcagaggt cccgtcttta
    10681 tcttgattta gtatcctctc cgcacctaac gccaccttat cgccataaat attccctgtg
    10741 ccgatattgt ggatttcttg agctttaatc acagtttgag cgttatcgtt gtccgagcgg
    10801 ctattgatta agccttcgtt atagacggtt gaagttgcag tcaaacgggt atctgccgag
    10861 cttattcggg cattttgtgc attggttatt tggtttgccg tgagctgcaa gcggtcattt
    10921 gcggctaatt ttttgcgatt gagaatatcg ccctcagtct gaataatcat cgacctattc
    10981 gcattgatat ctttggtaat atcaaagttc tcttgcaaat tcaggcgaat atcgcctgct
    11041 tgtacatcac catcaccgga taacgctttg gcagtgatct ccaaaccatt atttgcagaa
    11101 acttttccct cggtattcgt cattgtgagt gattttccgc cgaaaatagc cagatcgccc
    11161 caacttaaaa tttccccatt acggttattt aagctgtcgc tgagggtaaa tcgtgctgat
    11221 gagccgatat aaataccgcc tttttcatta ttgagctgtt ttgtgctgtt cagtacaaac
    11281 tgcgatgcaa taatcccctg tattggctga ggttggcgtg ccaaggtacc ttgattttcc
    11341 acctcgaaac tgttcatcgt aagtgagcca cctgcttcaa taagtgagcc attttccgag
    11401 atccgattat ttaagacagt cggtacattg agcgtgatat ctgtggccga tttcagtacc
    11461 ccaccttggt tatccaaaag cgtgctattt agggtgagat ttcccacact ttgaataaca
    11521 ccgtgccgat tatctacttg ttgggaaacc gtgagcgcta actcttttac tgaactaatt
    11581 tctccctgct cacggttatc taaggattga gcgttaatcg caacaccgct ggcggaaatt
    11641 cgcccgcctt gattttgcag ttcgctagtt atatcaaggg tttgctccgt ttgagaggtg
    11701 atcacccccg attgattttt taacgtattg accgtaatcg cctgtttttc gccactcaaa
    11761 atcagacctt gctgattatt cagcaatgga gcacgaatag ataactcgcc ttttgcaacc
    11821 aatttgccat cgactgaatc cacagcatca tttaaattta gccctaaacg cttgccacta
    11881 cttaatagcc cttggttatt tgttaaacga ttaccgtcaa tctcaaaatg tccacccact
    11941 gaaaagcgag ccgagtcagt atggatactc tctgctttta ggcgaaaatc ctgctcacca
    12001 gtctgcttcc aaaccgagcc acgagcattt aaattcgctt gttgggcctc aatcacctct
    12061 gcggttagct ggcttccttc agtggaaagc cattttgggg tggtgagctt gatctgattt
    12121 gcactaaact gcgcactatt cgcctgaata tcgcccgatt ttgcactgta ttcaacggta
    12181 tgggcattat tttggctctg attggcatca atctcagctg ccgccacgtt aattttcccc
    12241 gaagcgatat tttttccctg cacagtggtt ttttctgggg cagtgagttg aatagttttc
    12301 cctgccacag attgagcttc cagcttccgt acttcacctt gtgcagtgtc ggttacgtcc
    12361 accccagcgg caatcagtga gtttttgtcg gcgtgaattt ggcgggcggt gtaggtgata
    12421 ttgccttttg cgaccgtttc gccactttgc tggattttag tttcggcttt tttctgaata
    12481 ttgccacccc tcgccacgat ggaaccgcta ttttgaatat cggctttcga gttaagctga
    12541 atattgccct gtttattttc aattttgcct tggttgtcaa tcccgtgttg ggcattgagt
    12601 tgcacttgtc tttgagcgtt gaacgtgccg ctgtttacaa tccgcccttg actgtcaatg
    12661 gtgagcgtat cggcacttgc accgatatgc ccagcgttac gcacgcccac gccctgttcc
    12721 gtaccaatca ggtggatttt gccggcatac attccgccca gctcacccac atcaatcgca
    12781 actgtgggct tttcttcccc tgcaagcggt tgatttgtat gaataatttg caaattgttg
    12841 tccgaatttg accgcttgac ggtgtttttg ccggtaatga ctttagtctc ttttttcgac
    12901 cagacaccgg cgttgatttt cgcttctcgg gcgaggatat cggtgtaatc cactcggctg
    12961 ttgtccagcc ctttgccagc aaccgttacc ttgccttttt ccactacaaa accttctaaa
    13021 ttgccttgat ttatctgtgg ttttccggtg gtcagcgtgg ctcggtcggc gttgataata
    13081 ccgcagccgt cacagtgcaa gccgctcggg ttggcgataa tcacttccgc ttttttgcca
    13141 gccacttcca cataaccttt cattaccgaa ggcttactgg aattgacttc atttaaaatc
    13201 accttcgctt cgccacgggc aagatacggg ttgccttgca ccataccggc ttgttgggtg
    13261 gcggtgttgg tgcggctgtt gttgagaatt gcccccttct ccgctacgtc aaattggctg
    13321 tatttattgt gggaaagccc tttatcgttc ggggtttgaa tattgatttg tggcagcccg
    13381 ttggcggttt gcaaaataat cggttgctga tttttcgggg cagtgctatc ggcttgaata
    13441 atcaaagttt ccgccatagc gttgtctgat aaggtaacga atcccaacgc acagaacaag
    13501 ctgaacgcaa gcggtcgaat tttgcaaatt ttttgtaaaa ttgcaccgct tgtcatactt
    13561 tcactttctg atttacctgc taattttgct aattcagata ccaccacaat acgttgtaag
    13621 gtcttgctga aaatagtgcg aaaacaatgc ttattcataa t
//
LOCUS       DANLUA010000053        13588 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLUA010000053 DANLUA010000000
VERSION     DANLUA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13588
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            33..587
                     /gene="srlA"
                     /locus_tag="PXB44_001101"
     CDS             33..587
                     /gene="srlA"
                     /locus_tag="PXB44_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL5281590.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            618..1619
                     /locus_tag="PXB44_001102"
     CDS             618..1619
                     /locus_tag="PXB44_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL5281591.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            1664..2062
                     /gene="srlB"
                     /locus_tag="PXB44_001103"
                     /gene_synonym="gutB"
     CDS             1664..2062
                     /gene="srlB"
                     /locus_tag="PXB44_001103"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL5281592.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            2110..2898
                     /gene="srlD"
                     /locus_tag="PXB44_001104"
     CDS             2110..2898
                     /gene="srlD"
                     /locus_tag="PXB44_001104"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL5281593.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            3067..3447
                     /gene="gutM"
                     /locus_tag="PXB44_001105"
     CDS             3067..3447
                     /gene="gutM"
                     /locus_tag="PXB44_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL5281594.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            3510..4157
                     /gene="hxpB"
                     /locus_tag="PXB44_001106"
     CDS             3510..4157
                     /gene="hxpB"
                     /locus_tag="PXB44_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL5281595.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            4200..4997
                     /locus_tag="PXB44_001107"
     CDS             4200..4997
                     /locus_tag="PXB44_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL5281596.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            5008..5943
                     /locus_tag="PXB44_001108"
     CDS             5008..5943
                     /locus_tag="PXB44_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL5281597.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(5987..6537)
                     /locus_tag="PXB44_001109"
                     /pseudo
     CDS             complement(5987..6537)
                     /locus_tag="PXB44_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6839..8698
                     /locus_tag="PXB44_001110"
     CDS             6839..8698
                     /locus_tag="PXB44_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL5281598.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            8802..9941
                     /locus_tag="PXB44_001111"
     CDS             8802..9941
                     /locus_tag="PXB44_001111"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL5281599.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            9953..10468
                     /locus_tag="PXB44_001112"
     CDS             9953..10468
                     /locus_tag="PXB44_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL5281600.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            10608..12473
                     /locus_tag="PXB44_001113"
     CDS             10608..12473
                     /locus_tag="PXB44_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL5281601.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(12763..13014)
                     /locus_tag="PXB44_001114"
     CDS             complement(12763..13014)
                     /locus_tag="PXB44_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281602.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(13026..13508)
                     /locus_tag="PXB44_001115"
     CDS             complement(13026..13508)
                     /locus_tag="PXB44_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5281603.1"
                     /translation="MKIECKKVIGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAW
                     EADTREPPISKLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVV
                     VIKAKLQEAIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA
                     "
BASE COUNT         3854 a         2652 c         3100 g         3982 t
ORIGIN      
        1 aaaatatttt attttgaaaa acggagatcg gtatgattga agcaattaca cacggagcag
       61 aatggtttat cggcctgttc caaaaaggcg gtgaaacctt agtcggtatg ataaacggta
      121 ttttgccact cttaatttca ttattggttg taatgaatgc cctgattaaa tttgtagggc
      181 aagatcggat tgaacgactt gctcaacgta gtgccggaaa ccctatttca cgttatttga
      241 tcttaccggt gatcggcact ttcgttttct gtaacccgat gacattaagc ttagggcgtt
      301 tcttaccgga gcgttacaaa ccaagctact acgccgcagc ctcttatagt tgccactcta
      361 tgaacggctt attccctcac atcaacccgg gtgaattatt cgtttattta ggtattgcca
      421 gcggtttaac caccttaggc ttaccacttg ggcctttagc catcagttat ttattagtcg
      481 gtatgtttac caatttcttc cgtggctgga taacagactt caccaccgca ttctttgaaa
      541 aacgaatggg gatcacctta gatagagaag tacatttatc ccactaacca agtaactgaa
      601 atacagagga atagattatg agcaacaaag cgatttacat tgaaaaaggc aacggtggtt
      661 ggggtggtcc attaacatta cctgttgtgg aaggcaaaaa agtgctttat atgaccggcg
      721 gcactcgccc tgcgattgtt aataaattgg tggaattaac cggctgggaa gcggttgatg
      781 gttttaaaga aggcgaaccg ccggcagaag aaatcgggat tgcgattatt gattgcggtg
      841 gggtattacg ctgtgggctt tacccaaaac gccgtattcc aaccattaac attcactcca
      901 ccggtaaatc cggcccttta gcagaattta ttgtggaaga tatttatgtt tcagcggtga
      961 aagaaaacaa tattcgctta attgatgccg atggaagtgt gcctgtggca acagagcaag
     1021 caaaaccaac ttcatcaacg acagcaacgg caaaagagta tgactccagc aaaaaaatca
     1081 ccgaacaaag tgatggttta ttagcaaaaa tcggtatggg aatgggatcg gtggcggcgt
     1141 tattcttcca agccggacga gacactattg atacggtgct taaaaccatt ttaccgttta
     1201 tggcatttgt ttctgcgtta atcgggatca tcattgcgtc aggtttaggt gatgtgattg
     1261 cccacggctt gacccctctg gcaaacagcc caatcggttt agttacactg gcgttgattt
     1321 gttccttccc tctcctttcc ccattcttag ggccgggagc cgtgattgca caagttatcg
     1381 gcgtgttagt cggtacgcaa atcggtttag gcaatattcc gccacattta gcactgccgg
     1441 cactctttgc tattaatgct caagccgctt gtgattttat tcctgtgggt ctttcaatgg
     1501 cggaagcaaa acaagatacc gtacgggtgg gtgtaccgtc agtattagtc agccgttttt
     1561 taaccggtgc accgaccgtg ctgattgcgt gggcagtttc cgcattcatc taccaataat
     1621 taaccacgat acaagcggtg gaatttgaaa aattttttgc aaaatgcacc gcttgtcaaa
     1681 acgagagaac atttatatga cgattattta tcaaacgacc tttaccaaaa tcgggaattt
     1741 tgctcaggaa tcattaacag acgatatgtt aatcactttt aaacaaggcg caccggcaga
     1801 tttacaagat tactgcttca ttcataatcc atctgaatta agtagccctt tagaagtagg
     1861 cgatatcgct gaatttgatg gcgtagctta tccgattacg gcagttggta gcgttgcatc
     1921 tgaaaactta tccgccctcg gacacattac cttccgtttt gacggggcaa atgatgcgga
     1981 atttccggga agcgttcacg ttatcggaac accgccgcaa ggcttaacag agaactcaac
     2041 cttgattatc aagcgtgatt aatgacggat taaaaataat ttcaaattga aaatttagag
     2101 gaatacatta tgaacaatga aaaaaaagtt gctatcgttg ttggcggcgg tcaaacactt
     2161 ggagcttttt tatctgaagg cttggcagat gccggttatc gtgtggtggt tgctgactta
     2221 aacggcgaaa atgcacaaaa agtggcagcc gacattaatg ccaaacaagg cgaaggaaat
     2281 gcgattgggg tgcaagtcga tgcggctgat gaaccaagcg ttgaagcact agctaaagca
     2341 acagataccg tattcgggcg tgccgatgta ttggtttaca gtgcaggcac agcgaaagca
     2401 tcgcctattt gggatttcaa cttaaacgat tttgatttgt cggtaaaagt gaatttaacc
     2461 ggttatttct taaccgcaaa acacttctcc cgcttaatga ttcgtgacgg tattaaaggg
     2521 cgtatcattc aaattaattc taaatcgggt aaagtaggga gtaagtataa ttccggttat
     2581 agtgcagcga aatttggtgg tgttggttta acacaatcgt tagcattaga cttggcagat
     2641 aaaggcatta ccgtgcattc attaatgtta ggcaatttat tgaaatcgcc gatgttccaa
     2701 tcgttaattc cgcaatatgc ggcaaaactc ggcattccgg aaagcgaagt agagcaagtt
     2761 tatattgata aagtgccttt aaaacgtggc tgtgattatc aagatgtgtt gaatgtctta
     2821 aaattctatg ccggcgaaga ggcagcttat tgcacaggac agtctattaa tattaccggt
     2881 ggtcaggtaa tgttctaagc aaacaagcgg tcaaatttgt cgattttttt acaacaattt
     2941 gcaaaaaatg actaaaataa accgcttgtt aattcgccac accggttaat atgccacagg
     3001 ggaaagcgta ttgttttgaa atcgcaggta cgtttaggct ctgtggcttt ttattaagga
     3061 gcaatgatgg attcaattac acttctgatt atattggcaa tgataatgtg gctgttacaa
     3121 gccgttttgg gttggcttca aattaaagcc tttaaccaag cctttatgga aatcagtaag
     3181 aaaggcaaag taacggttgg gcgtaatagt ggtcgtttta atcctaaatc ggttattgtg
     3241 ttggcgtttg atgatgagaa aaaagtggta gatagccttt gtatgaaagg attttccgtc
     3301 tttgccagac cgcagaaatt agcgtctgtg attggtttgt ctcttgatca aattgtgccg
     3361 gaaaaaatct ttccaagcga taaacgttca caatttgcct taaaagtggc gatatcttcc
     3421 ggatatagtt gctcagatca aaaataatgc gattttttat ttcattttga atattttttt
     3481 agttaaaaat gagataatgg agaataaata tgcagatcaa tagtgtcatc tttgatatgg
     3541 acggggtaat gattgattca gaaccccaat gggcagaggc tcaaattgca gtattagccg
     3601 acttaggtat taatatcacc gagcaagatt gcgaacaact caccagaggt aagcggattg
     3661 atgagttgag cgacacttgg attaaaaaat ttcagttatc tgtttccaac caagcattaa
     3721 gtgaagccat tttggcacac ggttgtgctg cgattgaaca acacggcaaa gcattaacgg
     3781 gtttatatga attattggat ttcttaaaat ctaatcagat caagttagcc gttgcaacct
     3841 catcgccgcc ggtggtgatt aaagcggtgt ttgatcgctt aaaactgtgg gattatttcc
     3901 cgattcgatg cagtgcaatg gacgaagaat atggcaagcc acaccctgcg gtctatttga
     3961 gtgcagtgaa aaaattaggc attgaaaaac aagagtgtat tgttattgag gacagtgtaa
     4021 ccggcttaat tgccgccaaa gccgctaatt taactacatt tttggtgaat accgattatc
     4081 aaaatgaaaa attttcaatt gccgataaaa gaatggcatc gctactagat gtcattgaaa
     4141 aattaaaaca gcattagcac agattagctc gctccaatct ttgctaaatt ggaggaacaa
     4201 tgaaaccttt tgaaagaaga cagcaaattt tagcctattt agctcagcac ggtagaaccg
     4261 aagtggacgt gctggcagac tattttaagc taactggtgc aaccattcgt aaagatttaa
     4321 cttcacttga agaagataag aaagtcttgc gtacttatgg cagtgtagtc ttaattgatg
     4381 aagaaattct tgaacgggaa gagaaaatca ataaagagga caaagacgat aaaccgcttg
     4441 cacagaaaac cggcattaat cttcaacaaa agcaagctat cgggcaaaaa gctgcagagt
     4501 taattaatga gggaaactct attatttttg atgcaggcag cacagtgtta caaatgattc
     4561 cccatttaaa agcatttgat aacctctctt taatgacaaa tagtttaact attttcaatg
     4621 agattttgaa actcaataaa tcctataact tactgatttc cggtggtaca tttcgtgaga
     4681 aatcagcttc atttcacggt tattttgctg aatccgcttt tattggttca aattttgaca
     4741 tactctttat tggcacagac ggactagatt tagacgttgg attaacgact ttcaatgaag
     4801 tatataaagt cagttcgcta atgtgtgatg cggcaaataa aatcgtctta cttgccgact
     4861 ccagtaagtt tggacgtaaa agtccgaata ttgtgtgtgg gttagaaaaa attcatacta
     4921 tcattaccga tgaaggatta tctcaagaaa tgagagaaca gattaaagcg aaagggattg
     4981 agctgttagt tgtataaggg cgtaaaaatg aatcatttag acagtgcaaa agaaacgtta
     5041 aacctctatg ctcagcagat tatcacgcta aaccatcgac tttctgaaga atttaatgag
     5101 gtggtaaata tgatactcgc ttgtcaaggg cgagtggttg ttggcggtat tggaaaatcg
     5161 gggctggttg gcaaaaaaat ggtggcaact tttgcctcca ccggtacacc gagctttttc
     5221 ttgcacccga cagaggcttt tcacggcgat ttgggaatgc tgaaaccgat tgatatggta
     5281 attctgattt ctaacagtgg tgaaactgat gatgtgaata aactgattcc aagcctgaaa
     5341 aatttcggca ataaaattat tgcgatgaca ggtaatcctt actccacact tgggcgaaat
     5401 gccgatgtga ttttaaatat tggtgtagag cgtgaagcct gcctgaataa tctagctcca
     5461 accagctcta ctttggtaac gatggcttta ggcgatgcgt tagccattgc gctaatgaaa
     5521 gcccgtgatt tccgccctga agattttgcc cgctaccacc cgggtggcag tcttggtcgt
     5581 aaattattga acagagtgcg agatgtgatg gtgcgtaaag tgccaaccgc cagcttagac
     5641 accaccttta ccgaatgttt atctgtgatg aacgaagggc gtatgggggt ggcggtaatt
     5701 atgcaagacg ataaattgga aggcattatt accgatggtg atatccgccg cacattagcg
     5761 aaattcgggg cggagagcct aaataaaacc gctgacgaaa ttatgacccg taatccaaaa
     5821 accattaacg ataccgaatt tttagccaaa gccgaagatt taatgaaaga gctgaaaatt
     5881 cactcattaa ttgcagtgaa tgatgagggt aaagtaacgg gattgatgga gttctctagt
     5941 taatttatag atgtaacgat ggcgcaagcg tttacgcttg tgccgttcac tgacatatat
     6001 caatatctaa taaaacaggt aagttgttag cataatttct tgcactgcta tatttccatt
     6061 caaatgcctc gcatacaaaa ccgcttacga tagggttgtt gtgaatatgg tttagctttt
     6121 catcaaaaaa tttctgagaa tagattgtta tcggttggtt atgatgttgc caaagttggt
     6181 attgcttgac attatttcgt tgtttagctg cccgagtaaa catccataat aaccattctt
     6241 ttctactttc ttggggattt gttttaattt gctgaatcaa catttagatg taaatgtttt
     6301 aaaatctctt attaaatctg acgggttttg gtcttttgct tgaaataata aatgaatatg
     6361 gcttggcata atgcagtagc catataaaat catccctttt tgttttcgac aataattcaa
     6421 tgattggata atggtttgaa aataatcttc tcgaataaat agatcaatcc aataaactgt
     6481 tgcaaaacta acaaaatagg ctccgctctt ctcataaaac ttatattttc tactcattat
     6541 gaacctctaa agtgcagttt atgctgatag taatataggt gaacagttag ggttagcaat
     6601 tcattttatt ttttcgatgt agatcgcaaa aatagaatta tgcttggcac aagcgaggac
     6661 gcttacgcca tctttactcc aaaaatgtga gatagatcac attttcaaat ctgcccaaaa
     6721 aaatttaatg tgatctactt cacatttctg agagaaacat ttttgtaact tttgaaagtg
     6781 gtgctaattt aagcacgatt ttttcaaccc taattttctc tccttttttg aggtttctat
     6841 gtctaattta aaagtgaaag tccaaggttt tgggcgtttc ttatcgaata tggtgatgcc
     6901 gaatatcggt gcctttattg cgtggggctt tatcaccgcc ctgtttattc caaccggttg
     6961 gttgccgaac gagacttttg ctaaactggt cggtccgatg attacttact tgctgccact
     7021 gttaatcggt tacagcggtg gtaaattagt cggtggtgag cgtggtgcgg ttgtaggtgc
     7081 gattactaca atgggtatta tcgtgggttc tgaaatcccg atgttcttag gtgcgatgat
     7141 tgtaggtcca ttaggcggct gggcgatcaa aaccttcgat aaagcgattg acggcaaagt
     7201 aaaaagcggt tttgagatgt tggtaaacaa cttctcggtc gggatcatcg gtatgatttt
     7261 ggcaatgctt tcatttgctt tcattggagg tatcgtcacc caaatctctg cggcattagg
     7321 tgcaggtgtg ggggcgttgg ttgaagctgg tcttctgcca gtaacctcta tcatcgttga
     7381 accggcgaaa atcctattct taaacaatgc gatcaatcac ggtgtgttca cgccattggg
     7441 tacagcacaa gcacaagaag tgggcaaatc tattctgttc ttgattgaag cgaacccggg
     7501 tccgggcttt ggtatcttgc ttgcctatat gttctttggt cgtggcacag caaaacaaac
     7561 cgctggtagt gcgtcaatca ttcacttctt aggcggtatt cacgaaattt atttcccgta
     7621 tgtgttaatg agcccacgct taattcttgc agtgattgca ggcggtgcaa ccggcgtttt
     7681 aaccaactca ttattaggtg gcggtttaat cgcaccggct tccccgggtt ctatttttgc
     7741 gattttagca atgaccgcac cgggtgctca ctttgcagtt gttgcctccg tgattctttc
     7801 ttgtgccgtg acttttgcga ttgcgtcaat cttactcaaa acccaaaaag gtgaagcctc
     7861 attggaagat gcacaagcac aaactcaagc gatgaaagca gaaagcaaag gcgtagtaaa
     7921 tggtaacatc aacaaaatct atgtagcgtg tgatgccggt atgggttcaa gtgcgatggg
     7981 tgcgagtatg ttacgcaaaa aagtgcaagc ggcaggttta aacattgaag tacaaaactt
     8041 agcgatcaac gacttaccgc aagacgcaca actggtgatt acccacaaag acttaaccgc
     8101 tcgtgcaaca caacgtgtgg caacggctca acacttatcg ctcactaact tcttagacag
     8161 tgcgttctat gacagcttag tggcaaaact ggctaaccac cagccacaac aagcggtgca
     8221 aaatacacaa aattttgcaa acccggtgga agaacctcaa gccgaagggt taaaattgca
     8281 agcagcccaa gtctttttag gcttacaagc taacagtaaa gaagaagcga tccgctttgc
     8341 cggtgagcag ttagttaaag cgggctttgt tcaaccaagc tatgtagatg caatgttcgc
     8401 ccgtgagcaa atggtttcaa cttacttagg cgaaggctta gctgtgccac acggtacatc
     8461 tgatgccaaa gacagcgtac tgaaaaccgg tatcgtggtc tgccaatacc cgaacggcgt
     8521 tcgcttcacc gatgaagaag acggcgtggc gaaactggtg gtcggtattg cagcaaaagg
     8581 caacgaccat atccaaattt taagtgcaat taccaacgca ctggacgatg aacaagcaat
     8641 gaaaacactg acctcaacaa atgacgtgga aaaagtgtta gcattattat ctaaataatc
     8701 accctgttgt gtaggggcgg gtcctgtgcc cgcccattat cctacaaaat ttagggcggg
     8761 cacaggaccc gcccctacgt tttatagagg aacaatcatc aatgaacgca ctccattttg
     8821 gtgccggtaa tatcgggcgt ggctttatcg gcaaattgct tgctgatgct cgcattttcg
     8881 tgacttttgc cgatattaac caaactcaaa tcgaccaaat caaccaaaac aagcaatacg
     8941 gcgtgaaaat cgtgggcgat gacagccgtg tggaaattgt caaaaacatt gcggcaatta
     9001 actcgaaaga cgaaaatgcc gtgattgagc aagtgaaaaa caccgattta attaccaccg
     9061 cagtcggccc gaatgtgctt ggctttattg ccccactgtt cgccaaagcg ttagtggctc
     9121 gtgtggaaag tggcaacacc caaccgctga acattatcgc ctgtgaaaat atggtgcgtg
     9181 gcacaacttt ctttaaaggt aaaatttttg agcatttaac tgccgagcag caagcagaaa
     9241 ttgaaaaagt ggttggtttt gttgatagtg cggtggacag aattgtacca cctgccgagc
     9301 cgaaccctgc tgatccgttg gaagtcacgg tggaagagtt cagcgagtgg attgtggatc
     9361 aaacccagtt caaaggtgac attccaaaca tcaaaggaat ggaattaacc gataacctga
     9421 tggcgtttgt ggaacgcaaa ctgttcaccc tgaacaccgg gcatttaatc tgtgcctact
     9481 tgggcaaaca agcgggcgtg aaatggatta aagaggcgat tgcgattgag gaaatcaaaa
     9541 ctcaggtgaa agccacaatg gaagagagcg gtgctgtgct gattaaacgt tacggcttcg
     9601 acccacaagc ccattcagcg tatatcgaaa aaatcctcaa acgttttgcc aacccgtact
     9661 taaacgatga cgtaaaccgt gtagggcgtg agccaatccg caaactgagc gaaaacgacc
     9721 gcttgatcaa accgttgcgt ggcacgttgg aatatggctt accgtatcaa aacttggtca
     9781 aaggcgtggt gatggcattg caatatcgca atgaggaaga cccacaagcg gtcgaacttg
     9841 ccgaatttat tgcaaatcac ggtgtggcgg cagcggtgga aaaatacacc ggtttaaccg
     9901 accaagcggt cattgctcaa gtggtggaat tatatcaata attcttgaca ctatgatgga
     9961 ttacatcgac aaactcagtg aggaaaccac cctgcgtggt ttccttagca ttgccaatca
    10021 acgctttgcc aaagcggttg aacagttgat tgaacgagtg tttcgcaaaa cggactttgc
    10081 gttaaaatcc gtggtggatt ccttgtttga acatcaagga ccgctggctg acattgaagt
    10141 acgtttaaaa gtgttgcttg ggttgggggt gatctcacct aaagtgtttg aagatatctc
    10201 gcttttcctt gaagtcaaac agcatttgaa tgaggaagaa gacgagttcg cattttctca
    10261 cccggcggta gtgaaatttg ccaaagattt gcaccatatt gattttttgc ctatcaatga
    10321 attgctgaat atgaaagcga gtgcagcgaa taaagattcg atgctctttc aaatgcagca
    10381 aatgcggttg gaaaaaatgg tacgttcaag cctgattttg gcggtaactg aaattgacga
    10441 acaactgaat gtggaaagcc cattgtaaaa atttcgtcaa aactaaccgc ttgtactgag
    10501 cattcacgcc gtacgtgatt gctttttcct ttctttttga tcttatcggc ttttttcttt
    10561 gcttaaagcg gtgtaatcta tccctaacta ttttggagga tatggttatg caatcgttgc
    10621 tctcttttgt gccggatttt cttaagcaag aactgttttg ggtaatggcg ttattggtat
    10681 ttgcgatttg gcaatttatc caaaacaagt tgcgtatgga cgtggttgcc ttaattgtaa
    10741 tgctgttttt tagcctgact aatattttat cagtcaaaga agtgttggca ggcttaagtg
    10801 acccaaacgt ggtgctgatt gcgttgctct ttattgtcgg agaaggtttg gtgcgaaccg
    10861 gcgtggcgaa ccaagtcagc gattggttga tgcgggtgtc gggagcgagc gaaaccaaag
    10921 tgctggtgct gctaatgctc tgtattgccg ggcttggatc ttttatgagt tccaccggca
    10981 ttgtggcgat tttcattccg gtggtgcttg ccatttgctc gggaatggga atttctcctc
    11041 gccggttaat gatgccgtta agtgttgccg gacttatcag cggtatgatg accttaattg
    11101 ccacaccacc caaccttgtt gctcattccg aattagtaaa atcaggtttt gaaggcttca
    11161 atttcttcag ttttactccg attggattaa ttattttagt gatgggcatc ggctatatgc
    11221 tgattgcccg ccgttggttg gataatggtg agcaaagcaa tactcacagt caggctcaaa
    11281 aattttcaat gtcgcagtta attgaagagt atcaacttaa agagcgagcc aaaatggtgc
    11341 tggtggaagc agattcagac tgtgtcggca aatctatcgg gcaactgaat ttacggattg
    11401 agcaagggct aaacatcatt gcaattcaac gcactaagcg gtttagaaaa attaccgtca
    11461 gtgcctcggt aaacgaaatt ttccagccga aagatatttt actgcttgat actgccctcg
    11521 atgatgaaac ttaccaacaa cgctgtgaac agctcaaact tcgcccgatt gagctaaaag
    11581 gcgaatactt ttcctcacaa tatcgctcgg tgggtatggc ggaaatttcg gttatgcctg
    11641 aagcagaatg tatcggtaaa actattcggg aactcaaatt ccgctcaaga taccatttaa
    11701 gcattgtcgg cttaaaacgt ggtgatgaaa tcatacaaga ggaactgctc gatacgccac
    11761 tcaaattcgg cgatatttta ttggtgatgg gcgtatggca acaaattcac cgtatggacg
    11821 acgatcatcg agatttcttc ttgcttagag atccggcaga aagtaaaaac gcccctccgg
    11881 cgttaagcca agcccctcac gctctgttct cagtgctgac tatggtgttg ctgatgatta
    11941 ccggcattgt gccaaacgtg atggcagcgt tgattgcctg cttaatgtta ggcaagttcc
    12001 gctgtgtgga tatgaaaagt gcttatgatt cgattcatat gccaagcatt gtgttgattg
    12061 tggggatgat gccgttttct attgccttac aaaaaaccgg tggtgtggat ttaaccgtta
    12121 ggcttctact tgatgcaatg caaggcttag atgtacattt tgttctaata gtattatttg
    12181 tagtaacagc gattatcagt gcttttattt ccaacacagc gactgcaatt ttggttatgc
    12241 cgatagccat tgcgattgct aaccaacttg gctactcacc ggcacctttt gtgatgacgg
    12301 tcgctgtttc agcctccgcc gcttttatga cgccggtttc ttcaccggta aatacgatgg
    12361 tattagcacc ggcaggctat aaatttattg atttcgttaa agttggtgtg ccgtttactg
    12421 tactggtgat gctggtgtcg gtgtttgtga ttccgatgtt atttccgctc taaacactcc
    12481 tctaaattac aagcggtcat ttttggtaat ctgacgttga cggtaaaatt gttgagtatg
    12541 tctggcaaca tagtgtgggg tactgatctt ttttgcatag agagaaagaa aaaaatttgg
    12601 gcggaaaaat aacggcgagg ctaggaaata caaggcttcg ccgtttttgt ttggggtgag
    12661 ttatgcaaaa aacgaaaatg agcaaaaata gaaaaaaatg ggctattttt acgttatttt
    12721 ttgcataggt ctgagcaggt ttaaacggat tttaaaacaa ttttaagcgc cctcatctcg
    12781 gctggctcgg ttgcgttgct ctaagctgtg aatgaggtcg tgtacacgtt gtttatcggc
    12841 acaggttagg ttggtgttat tatccaccgt gcagccggca atcaccgtgg cgttatcgcc
    12901 gttttgaatg atgacgttgc tgttattaaa cacctgtata ggctcttggc ttggctggct
    12961 gttgccggca gtgctattgt ctctgaataa tttgagaatg gcttgtttgt ccatattgtc
    13021 ccctgttatg ccaccagttt gagtaatccc accatttttt ctttttcgct tttttcttgc
    13081 gtgagaagct cgtagaaagc ccacaccatt tgggcttttc cggcaggctt catttcccgt
    13141 tgggtaattt ccagcacctc ttccaacgtt tctattgcct cttgtaactt agctttgata
    13201 acaactactt ccccttcttc tgtctgcggt tttgtggcat acacaggctg tggctctctt
    13261 gtgacattaa ggctttgcga gtgtcgctca cctgttaata ggtagcaagg ctcaacgtta
    13321 agtatttgtg acaaagccat aagttttgat ataggaggtt ctcttgtatc agcctcccac
    13381 gcttgtaatg tagatattgc aatacctcct agggcatcac aaacctcaga tctacttaat
    13441 tttgcatttt ttctgctctg ttctattcgg ttaccgaaac caataacctt tttacactct
    13501 attttcataa ctgtaaaaaa ataagttgag atttttagcc ttttacactc taaaaaccta
    13561 taaataaatc aataacttac agcaaact
//
LOCUS       DANLUA010000054        13476 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLUA010000054 DANLUA010000000
VERSION     DANLUA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..513)
                     /locus_tag="PXB44_001116"
     CDS             complement(<1..513)
                     /locus_tag="PXB44_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281604.1"
                     /translation="MEHQNLTHLVYITSHKQIGVYSISNLNIDDLYFKGYCIQKNRVI
                     TLRADRIIKQFDDLALAENYAKNIPQDVFYLFDSLLNQQRKEKITPIYKIGLCFTGFK
                     QARKNELIQLAVDNDLRVVQNVTGAVDFLIFDKESKTVGPAKLAKAEKLGIKIIDDEE
                     FLYMLETGVVP"
     gene            complement(517..1203)
                     /locus_tag="PXB44_001117"
     CDS             complement(517..1203)
                     /locus_tag="PXB44_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5281605.1"
                     /translation="MDTLASRLKIVLDQKGLTQEKFAEQIGVSQPSVFKILNGQTRNP
                     TRIYEIANVLDVDVNWLKTGKGEAPDFAKIGKNPTAYEEESSMLRLEVLDVYASAGNG
                     SFVTGDLTAYTHAVEFENAYFAQVFQRANAKGLSIINVDGDSMEPTIGNGDLLFVDTT
                     KSAYQGDGVYVFSYGENLYVKRLQFAGDELLVISDNPLYKEWRITSENEHKFQIHGKV
                     EFMQGHIRRV"
     gene            1327..1539
                     /locus_tag="PXB44_001118"
     CDS             1327..1539
                     /locus_tag="PXB44_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5281606.1"
                     /translation="MTNKKIQKAIELVGTQTKLAEACGVSQPTVLKWLNGGGISAEYL
                     LKIEAATNGQVTIREICEELGNATAK"
     gene            complement(1638..1883)
                     /locus_tag="PXB44_001119"
     CDS             complement(1638..1883)
                     /locus_tag="PXB44_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281607.1"
                     /translation="MNTEDQIIELTVEVENLRHQLNAKNIILDDLKGRLTDYQRKRLD
                     RLFERSRRQYLNDLDLEEEQAEALEAICNELESFLAN"
     gene            2016..2288
                     /locus_tag="PXB44_001120"
     CDS             2016..2288
                     /locus_tag="PXB44_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281608.1"
                     /translation="MNLDHTCTNCKSKNLRVRTSEKIGLLTIQAVTYCNNCGTEQLVQ
                     SQIIRVRTPIYKERPEAMRINKCLMEADLNTPDLFDGVVADQPKQD"
     gene            2371..2703
                     /locus_tag="PXB44_001121"
     CDS             2371..2703
                     /locus_tag="PXB44_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281609.1"
                     /translation="MSKQLYYRHKSRWNRLNRLKKMNKRRLNLFLQEKRVANLESRLA
                     DEVELAKLNSEQIGDLLQALELKVNRLIRQNEKLKARVIKLEKVKQTQKQGLFAMFKK
                     VLGSKGNP"
     gene            2716..3009
                     /locus_tag="PXB44_001122"
     CDS             2716..3009
                     /locus_tag="PXB44_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281610.1"
                     /translation="MDFVATYQKIRINTNHKQFTCNHLGEIWGKENDDFKRFLLRVAF
                     LQDNPEPKPWGAYSPQEKRLLHSFSLWLEQIADFQRVMKRESKLREKHINAPD"
     gene            3161..3403
                     /locus_tag="PXB44_001123"
     CDS             3161..3403
                     /locus_tag="PXB44_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281611.1"
                     /translation="MSLITVNDYKIKKDILDADGKPCYQLYQGEELLRDTTNLSEIIA
                     YMFIRAEDKPFDELYNHIINQEAGAKTDIKFTVTVR"
     gene            3400..3732
                     /locus_tag="PXB44_001124"
     CDS             3400..3732
                     /locus_tag="PXB44_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281612.1"
                     /translation="MSQITKSAYTHIHPNWQAPRGNTYRIERYELKRRYALFCNGERV
                     VLYKDPTHPNKNVESHIESDVLLLWLLKNELGWSGNIKEPLSPQDVENIRLLEQAFCL
                     TYNGTFIK"
     gene            3729..6089
                     /locus_tag="PXB44_001125"
     CDS             3729..6089
                     /locus_tag="PXB44_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDL5281613.1"
                     /translation="MNYQNEILSATEAFAIGVSLPPVAGASACALELHSDRLYQAFKQ
                     PYTSMVQQELFSKDPIHEPLRQSYFERLPRALAEHFGRQYKQKLHYQSQYDTADWFKA
                     EMARKMPRIEAVISQYCDVFDFLKQSHQDLDFLAELDSNVYFGAGVDTQQAVQKIKQD
                     AEVKGIQLPMHLCRLENSSRYLKAHGIRPLAYQTEEQIKQLALGIAYRVRQIQQDNIE
                     QNMHKATDGDTAYSVLLECYRAMMGEVNKLKIDSPYQKKARKGRLTEEEITTGFLKMT
                     CEKWWTRKLSKIAEQMKEHLAIACGMVNMLSPYCSNARLKAFEAQRKANIDYLKSMII
                     ANIAEPEEQLSLFETWLKSASNPKIKRLELLTRMNGFERYADKQGHEGWFITLTAPSK
                     YHAMLSRTSSVNPKWNGASPAETQVYLVNTWAKIRAKLNREGVMAYGFRVAEPHADAT
                     PHWHLILFTRPEDMEKLRRVFLSYALEVDGTEAGAKKYRCKFKRIEKEKGSATGYLVK
                     YVSKNIDGFGMDGELSDEANIQAKENAARVGAWSSVWCIRQFQQLGNIPISLWRELRR
                     LGSVEQEDETLEKLRVIADSGEWDVFTEELGGALVKRADLVARITYTERKSETGEALY
                     TMYQEPSLKVSGIINIKNGVQINTRPKEWSIQLKPKNWEEQQLQKKLENATETERAEV
                     KRKYYEKLGFNEEAIALLGELAPPWTCVSNCTGSKNNQISEEARNCLRNELITMRGRV
                     TEHQIDDLLNGKRLKIWGNSQKTMFVSYSRGRLIEHIVDNYESFLN"
     gene            6102..6434
                     /locus_tag="PXB44_001126"
     CDS             6102..6434
                     /locus_tag="PXB44_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281614.1"
                     /translation="MVKFIRLNLINYFIEKDEYGEPVRCRAFRYDEIIQTKFIEGFRE
                     GEIPADDYGFEDWNGSVLILNEEGRKYFENYLFDVKKNYCRARDNYVYVLETLDEIEM
                     KLGLLEAK"
     gene            6434..6886
                     /gene="ssb"
                     /locus_tag="PXB44_001127"
     CDS             6434..6886
                     /gene="ssb"
                     /locus_tag="PXB44_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5281615.1"
                     /translation="MNLVTLVGRLGQDPDIRTMQNGEKAAALSVATSEKWTDKQTGVK
                     KESTEWHRVVLYRRLAEIAELYVKKGHLVSIIGKIKTRKWTDSNGVERSITEIIAEQM
                     QMLSSGEKNTPNTPNKAENKPQPKQKNQDVMTADEQKDIPQFDDDIPF"
     gene            6897..7229
                     /locus_tag="PXB44_001128"
     CDS             6897..7229
                     /locus_tag="PXB44_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281616.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            7219..7488
                     /locus_tag="PXB44_001129"
     CDS             7219..7488
                     /locus_tag="PXB44_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281617.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWIDDLGGKNGTITSGQ"
     gene            7463..7753
                     /locus_tag="PXB44_001130"
     CDS             7463..7753
                     /locus_tag="PXB44_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281618.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(8023..8205)
                     /locus_tag="PXB44_001131"
     CDS             complement(8023..8205)
                     /locus_tag="PXB44_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL5281619.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(8483..9481)
                     /locus_tag="PXB44_001132"
     CDS             complement(8483..9481)
                     /locus_tag="PXB44_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5281620.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            complement(9709..9785)
                     /locus_tag="PXB44_001133"
     tRNA            complement(9709..9785)
                     /locus_tag="PXB44_001133"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9749..9751),aa:Val,seq:gac)
     gene            complement(9849..10649)
                     /gene="apaH"
                     /locus_tag="PXB44_001134"
     CDS             complement(9849..10649)
                     /gene="apaH"
                     /locus_tag="PXB44_001134"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL5281621.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(10741..12717)
                     /locus_tag="PXB44_001135"
     CDS             complement(10741..12717)
                     /locus_tag="PXB44_001135"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL5281622.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            12877..13155
                     /locus_tag="PXB44_001136"
     CDS             12877..13155
                     /locus_tag="PXB44_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5281623.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            13180..13476
                     /locus_tag="PXB44_001137"
     CDS             13180..13476
                     /locus_tag="PXB44_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5281624.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
BASE COUNT         4050 a         2398 c         2996 g         4032 t
ORIGIN      
        1 cggcacaacg ccagtttcta acatatataa aaattcttca tcatcgatga ttttaattcc
       61 taacttttca gcttttgcta gttttgctgg tccaacagtt ttcgactctt tatcgaaaat
      121 taaaaaatca actgcaccag ttacgttttg tactactctc aaatcattat ctactgctag
      181 ttgaattaat tcattttttc tagcttgctt aaatccagta aaacacaaac cgattttata
      241 gatcggtgtt attttttctt ttcgttgctg gttcagaaga gaatcaaata gataaaacac
      301 atcttgagga atatttttgg cataattttc ggcaagagct aaatcatcaa attgtttaat
      361 aatgcgatca gctcttaatg tgataactct atttttctga atacagtaac ctttgaagta
      421 aaggtcatca atattcaaat tagaaatcga ataaactcca atttgtttat gtgatgtaat
      481 atagacaaga tgagttagat tttgatgttc catcacctaa accctcctaa tatgcccttg
      541 cataaattcc actttaccgt gaatttggaa tttatgctca ttttctgatg tgattctcca
      601 ctctttataa agcggattgt cggaaatgac taacaattca tcaccggcga actgcaagcg
      661 ttttacatat aagttttcgc catagctgaa aacatagaca ccatcgcctt gataagcgga
      721 tttagtggta tctacgaaca gcaaatcacc gttaccgata gttggctcca ttgagtcgcc
      781 atctacatta ataattgata agcctttggc atttgcacgt tgaaagactt gtgcaaaata
      841 ggcattttca aattctactg cgtgcgtgta agcggttaag tctcccgtga caaaactacc
      901 attgccggca gaggcatata catctaacac ctcaagtcga agcattgagc tttcctcttc
      961 ataagcggtc ggattttttc cgattttcgc aaaatctggt gcttcgcctt tgccggtttt
     1021 gagccaattt acatcgacat ctaggacgtt tgcaatttca taaatccttg tgggatttcg
     1081 agtttgtcca ttcaggattt taaaaacaga tggctgtgaa acacctattt gttctgcaaa
     1141 tttttcctgt gttaaacctt tttggtctaa aacgattttc aaacgacttg ccaaagtatc
     1201 cattaggaac tccttatttt tgatgagtat ataactaaag gtatagaaat aaaaattcat
     1261 ttggttattg attttttatt tctttggtta tattattata accattcaat aaccagagtt
     1321 atatttatga ctaataagaa aattcagaaa gctattgaac ttgtaggaac acaaacaaaa
     1381 ttagctgaag cttgtggagt tagtcagcct acggtcttga aatggctaaa cggaggcgga
     1441 atttccgctg aatatctact aaaaatcgaa gctgccacaa acgggcaggt aacaattcgt
     1501 gaaatttgtg aggagttggg aaatgcaaca gcaaaataaa agccctgaac aggcagggct
     1561 agcgagtggg aaatttactg taagacgtaa tcaagacggt aaatggcgtt tagatttgac
     1621 cttgttggat tttggtatta gttcgccaga aacgactcaa gctcgttgca aattgcttca
     1681 agagcttcag cttgctcttc ttcaaggtca aggtcattta aatactgtcg gcgtgaacgt
     1741 tcgaaaagcc ggtccagtcg tttgcgttgg tagtctgtta agcgaccttt taaatcatca
     1801 agaataatgt ttttggcgtt tagttgatgg cgtaagtttt caacttcaac agtcagttca
     1861 ataatttgat cttcagtatt cataaaattt ccttttggag ggttaaacaa tatctaaaac
     1921 ataaccaaaa ttcgactgct tgtctatggg gatagataag ccgaaatcaa cgtaaagaat
     1981 gttaatagag ccttggggaa ggcgtagggg aaataatgaa tttagaccac acctgtacaa
     2041 attgcaaaag taaaaattta cgggttcgta catctgaaaa aatcggtttg ctgactattc
     2101 aggctgttac ttattgtaat aactgcggca cagagcaact tgtgcaaagt caaattatcc
     2161 gtgttcgcac accgatttat aaagagcgac cggaagcaat gcgtattaat aaatgcttga
     2221 tggaagccga tttgaacacg ccggatttgt tcgatggcgt tgtggcagac caacctaagc
     2281 aagactaatc ccaattttta aacaatcgtc aaaataagcc ttatttgcat agggcgagat
     2341 ttttgcaccc taaattcagg aatttgaaca atgagtaagc aattatatta ccgacacaaa
     2401 tcacgttgga acagacttaa ccgccttaag aagatgaata aacgtcgcct taatctgttt
     2461 ttgcaagaaa agcgggtggc gaatttagaa agccggttag ctgatgaagt agagctggca
     2521 aagctgaata gtgagcagat tggcgattta ctgcaagcgt tggagttaaa agtgaatcgg
     2581 ttaattcgcc aaaatgaaaa gctgaaagcc cgtgtgatta agctggaaaa agtcaaacag
     2641 acccaaaagc aaggtttgtt tgcaatgttt aaaaaggtgt tgggtagcaa gggtaatcct
     2701 tagtaagagg tagatatgga tttcgtggca acgtatcaaa agatacgcat taacactaat
     2761 cacaagcaat ttacttgcaa tcacttaggc gagatttggg ggaaggaaaa cgacgatttt
     2821 aaacgtttct tactacgagt cgcattcttg caggataacc ccgagccgaa accttgggga
     2881 gcgtattccc cacaagaaaa acgcctttta catagtttta gcttgtggtt agagcagata
     2941 gcagattttc agcgagtaat gaaaagagaa tcaaaactgc gtgaaaaaca tattaacgca
     3001 ccggattaga aggagtaaat atgagccgta accgtgaatt gtgtgagttt tttcacaaac
     3061 tgtggtgcaa aaccggtaat cgctatgctt atcgccggta tcaagtgttt tttcagcgtt
     3121 atcaaaacga atgtttagtg aatgagtaga gggtagaaaa atgtcattaa ttactgtgaa
     3181 cgattacaaa atcaagaaag atattttaga tgcagacggc aagccgtgtt atcagttgta
     3241 tcaaggcgaa gagctattgc gtgataccac caatttaagc gaaatcatcg cttatatgtt
     3301 tattcgtgcc gaagataaac cgtttgatga gttgtataac catatcatca accaagaagc
     3361 aggagctaaa acagatatta aatttacggt aacggtgaga tgagccagat tactaaatcc
     3421 gcttacacac atattcaccc aaactggcaa gccccgagag gtaacactta ccggattgag
     3481 cgatatgagc taaaacggcg gtatgcctta ttttgtaatg gtgagagggt agttttatat
     3541 aaagacccaa cacaccccaa taagaacgta gagagccata tagaaagtga tgttttgttg
     3601 ttatggctac tgaaaaatga attagggtgg agcggtaata ttaaagagcc gttaagccct
     3661 caagatgttg agaatatccg cttgttagag caagcatttt gtttaactta taacggaact
     3721 ttcattaaat gaattatcag aatgagattc tatctgctac agaggctttc gcaataggtg
     3781 tttcactccc acctgttgcc ggtgcttctg cttgtgcatt agagcttcat tctgatcgtt
     3841 tatatcaagc gttcaaacag ccttacactt caatggtcca gcaagaatta ttctcgaaag
     3901 accctattca cgaaccgtta cgccaatctt attttgagcg tttgcctcgt gccttagcag
     3961 agcatttcgg cagacagtat aagcaaaagc tacattacca aagccaatac gacacagcag
     4021 actggttcaa agcggaaatg gcacgcaaaa tgccacgcat tgaggcagtt attagccagt
     4081 attgcgatgt attcgatttt ctgaagcaat cccatcaaga tttagatttt ttagctgaat
     4141 tagatagtaa tgtttatttc ggtgccggtg ttgatacgca acaagcggta caaaaaatta
     4201 aacaagatgc cgaagtgaaa ggtattcaac tgcctatgca cctttgccgt ttagagaata
     4261 gctctcgcta tttaaaagct cacggcattc gcccgcttgc ttaccaaacc gaagagcaaa
     4321 tcaaacagct tgccttaggt attgcttatc gtgttcgcca aattcagcaa gataacatcg
     4381 agcagaatat gcacaaagcg acagatggcg acacagccta tagtgtgttg cttgaatgct
     4441 atcgggcaat gatgggagag gtaaataagc tcaagattga ttcgccttat cagaaaaaag
     4501 cgagaaaggg tagattaact gaagaggaaa ttaccacagg ctttttaaaa atgacctgtg
     4561 aaaaatggtg gactagaaag ttatctaaaa ttgccgaaca gatgaaagag catttagcta
     4621 ttgcgtgcgg tatggtgaat atgctttcgc cttactgttc aaatgctcgc ttgaaagcgt
     4681 ttgaggctca acgcaaggcg aatattgatt atttgaaatc aatgatcatt gccaatatcg
     4741 ccgagccgga agagcaactt tcattatttg aaacgtggtt gaagtctgcc tctaacccga
     4801 aaattaaacg ccttgaatta ctcactcgta tgaatggatt tgagcgttat gcagataagc
     4861 aagggcacga ggggtggttt attaccttga ctgctccgtc taagtatcac gcaatgcttt
     4921 caagaaccag tagcgtgaac ccgaaatgga atggagcaag ccctgcggaa acacaggttt
     4981 accttgtgaa tacttgggcg aaaatccgtg ccaaactcaa ccgtgagggc gtaatggctt
     5041 acggttttcg agtggctgaa cctcacgctg acgctacccc acactggcat ttaattctat
     5101 tcactcgtcc ggaagatatg gaaaagcttc gccgcgtgtt cttgagttat gccttagagg
     5161 tcgatggcac agaagccggt gcgaaaaaat accgttgcaa attcaagcgt attgagaaag
     5221 agaaaggctc ggcaacgggc tatttagtga aatatgtttc taaaaacatt gacggttttg
     5281 gaatggacgg cgagctttca gacgaggcaa atattcaggc gaaagagaat gccgctcgtg
     5341 taggagcgtg gtctagcgtt tggtgtattc gacagttcca gcagttaggt aatattccga
     5401 tcagcttatg gcgtgagtta cgccgtttgg ggagtgtaga gcaagaagat gaaactttag
     5461 agaaactccg tgtgattgca gatagcggtg agtgggacgt tttcaccgaa gaattaggcg
     5521 gtgcgttggt taaacgtgcc gatttagttg cacgtattac ttataccgaa cgcaaaagcg
     5581 aaaccggtga ggcgttatat acgatgtatc aagagccatc attaaaagtg agcggcatta
     5641 ttaacattaa aaacggtgtg cagattaata cacgcccgaa agagtggagc attcaactta
     5701 aacctaaaaa ctgggaggag caacaattac agaaaaaatt agaaaatgca accgaaacgg
     5761 aacgagcgga ggttaaacgt aaatactacg agaagttagg gtttaacgaa gaggctatcg
     5821 ctttattagg ggagcttgct cccccttgga cttgtgtcag taactgtacg gggtcaaaaa
     5881 ataatcagat tagtgaagag gctcgaaatt gcctaagaaa tgaactgatt acaatgcgag
     5941 gacgggttac tgaacatcaa atagacgatt tactaaacgg caagcggtta aaaatttggg
     6001 gaaattcgca aaaaacaatg tttgtgagtt atagccgtgg gcgtttaatt gagcatattg
     6061 ttgataatta tgagagtttt ttgaattagg atattaagaa aatggttaaa tttattcgtt
     6121 taaatttgat taattatttc attgagaaag atgaatatgg agaaccggtg cgttgcagag
     6181 cgtttcgtta tgatgagatt attcaaacta agtttattga aggatttcga gagggagaga
     6241 taccagctga tgattatgga tttgaagatt ggaatggttc ggttctgatt ttaaatgaag
     6301 aaggacgtaa atattttgag aactatcttt ttgatgtaaa gaagaattat tgtagagcaa
     6361 gggataacta tgtatatgtc ttggaaactt tagacgaaat tgaaatgaag ttaggattat
     6421 tggaggcgaa ataatgaact tagtcacatt agtcggacgt ttggggcaag accctgatat
     6481 tagaacaatg caaaatggtg agaaagctgc ggctttatcg gtagcgacct ctgaaaagtg
     6541 gacagataag caaacaggtg tgaagaaaga aagcaccgaa tggcataggg tggtgcttta
     6601 tcgccgatta gctgaaatcg ccgaattgta tgtaaaaaaa gggcatttgg tatcaattat
     6661 cgggaaaatt aaaacccgaa aatggacgga tagcaacggt gttgagcgga gtattacaga
     6721 gattattgcc gagcagatgc aaatgctcag tagtggtgag aaaaatacgc caaatacgcc
     6781 aaataaggca gaaaataaac cgcaaccaaa gcagaaaaat caagacgtga tgactgcgga
     6841 cgagcagaaa gatattccgc agtttgatga tgatattccg ttttaggggg tgaataatga
     6901 gagaatggga aaaacaaatg ccatttagtg caaatttaca tataagacct acaggaaaat
     6961 ttgaagtgaa aaatgtaaac atagaacaaa taatcggaga acgtggcaaa aattacggca
     7021 actttgatga tgttgccaca atgagccagc agttaaaagc agtgttgctg gataccaata
     7081 ctcaacttaa accacatcaa aaagaggcag gcgagatgat ctgcttgaaa ctggctcgca
     7141 tttttgcagg ttgtaatccg gactatgaag ataactggcg agatattgcg ggttatgcgg
     7201 tgctgggagg gaaattaaat gaaaaataaa atggggagta aatttatacg agtcagagtt
     7261 gagcatccac agcggaaaaa agaagatatt gagcttgtgc aggatgtggt tgcttatcat
     7321 atttttaatc tttttaaatt tgaggaaagt gggttacggg ggtttagtca gtatgatttg
     7381 gaaattgatg tgaaagttaa gccagtgtta gaacaaaaag aaatgcctgt agatgtttgg
     7441 attgatgatt taggtggtaa aaatggaaca attacaagcg gtcagtaatg agcaagtttt
     7501 tgcaaaactt tgtgaggttg agcggttatt aaaaaccaaa ggggtgaatg agcatagccg
     7561 tgaattgtgg gatttgggcg atgtagcagc atatttcggc tacactaaag aacacaccag
     7621 ccgaagtgtt gtatctagtc cacattttcc gaaaccgatt gcccttgacg gcttacgagg
     7681 caagggcagg ggggcgaaga agtgggtatc cggtgaagtg gtgaaatttt gtttgatgtg
     7741 gaaggtgaag tgatgataaa aaagataatt ttagatgctt gttgcggtag ccgaatgttt
     7801 cactttaata agcacaaccc ctcggtattg tttgctgaca atcgggaatc cctgtatcga
     7861 aaattttaaa tgttatcgaa taccaaccta tctatgggca taaaagcggt aaacataaca
     7921 aaacgcattg gctggcattt gtaaagatgg ataataacga ttatttgtaa agtatttcaa
     7981 aatacaaaag ccgataaatt tcattattta tcggcttttg ttttatgggt tgttttgctt
     8041 gaattgttga atagcttgaa taatcagctg gttttgtgga atgttgagtt tttggctaag
     8101 tacttcgatt tctgcgatca cctcaagcgg tagtttaaag gatttttgct ttattcctcg
     8161 ttttgcctcg cttttagctt ggatttcatt tcttgacatt gccataattt atccctatta
     8221 taaattttgt tgaagagctg ggagctttca cccccagcga gttgcttcag attagtaagc
     8281 tggggagcta aggactaata agaagacaac taggataatg tatttaaaca ttgtgctatc
     8341 cttcttcatt tcgtaaggtt taagccttac gctcactttc aagctgtctc ttgaaagtga
     8401 ggttattata atgtagggta tcctacattg caagtaataa atacaaaaaa gccgataaat
     8461 tttcgtttat cggcttttgt ttttattgca gtttgctcac aagttctgaa atatccgggt
     8521 tatagtaggt attttgcaaa atacttaaat ctcgatgccc cgatactttg gcaagctcca
     8581 ttaccgtcag atatttagac aaacgtgtga gtgcctctcg ccgtgtgtcg tgaaaatgca
     8641 gatcggcatt gtgtagattg gctttttgtt tcagcttgcg aaaattcgaa tcaaggctgc
     8701 gactggtgag ctggaaaaca ctgttttgct tgttcggttc aagttgtgcc aactgattta
     8761 aaattgctat tgctttggtt gagagtggca cggtgcgtga ataaccgttt ttggtttgtg
     8821 gcaaaaatgc ggtgcgtgcc tgcaggttta tatgttccca ttttaaacca gcaatttcac
     8881 ccgctcgcat tgcagtctca atcgcaaata atagtgctgc tccggctctt tgttgcatta
     8941 atagcggtgg ctggttaggg ctgtagccgg agacaaaaat cagtttgtct atctcctcat
     9001 cgctatatcg gcgagttctt gctggtggcg ttttgatttt ttccagcgtt tttagaggat
     9061 tttctttgag ataatcccat tccattgctt ttgtaaagag tgccgataaa gtggctcttt
     9121 ctcttgagac ggaaaccggc tgcacttctt gcaagcgttg gttttgccat tggcgtaaat
     9181 gctctttggt gagtgccggt aatggtatat ttcccaagtt gctgccggca attcttagca
     9241 ggcgatagcg ttcttccctt ttgccacgtt tggtcggagt gacctctttg aggtatttgt
     9301 cgattaattc ggcaaaaaga atgttcgggg tggtgttata ttctccggca tcaatttgag
     9361 cctctaaagc gtaagcccag cggtttgcct ctgttttggt gctgaaagtg gcagttttat
     9421 aaatgccttt tttgcggact tgtgctcggt atttgttgcc gttcttaata atagtagcca
     9481 tttttttatt cctttgtgta tgtgtgcagt ttaagtgtgc aaatgtgtgc aaaggaatgt
     9541 taatagagat cataaacgat caataaatat caagttttca tatcaaaaat gatgttttta
     9601 ttgatgttat gctacacttg gggaagtgtt ttaaatcaat aagctattga tttataaggg
     9661 gtatttttgg ggttaagcct gaatggggat attcaggctt aaaaataatg gtgcgactag
     9721 ctggactcga accagtgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctata
     9781 gtcgcattaa cggtgtgcat tctattggaa aaaggaactt agtgcaatat gcttttggtc
     9841 gaatgctttt attttagacg cttttgggta aaaatttgtt tatcttccca acggagcatg
     9901 gttaaatggc ttccccaagc acagccggta tcaagtgcat agatgtttgg acgagtgcat
     9961 ttgcccatta ggcttgccca gtgaccaaag ataatgtttt tctgtgtaaa taacggatta
    10021 tctaagtcaa accaagccag cagttcttga ggggcttgtt caagcggtaa tttacagtca
    10081 aaatctaacc gcttgtcggt gtagcaaaac cgcatacggg taaacacatt gatgatataa
    10141 cgccaacgtt caacaccttg caaatcttca ctccagcgtt cgggcttatt ctcatacatt
    10201 tgggcgatgt agtccgcata attgtcactt tgtagcacct tttctgcctc tttggcacaa
    10261 gctagggtag ttgctaaatc ccactcaggg ctgattcctg cgtgagtcag caaaaattga
    10321 tgtttggggt gatgaattac aagcggttga tttctgagcc aattttgcaa atcgagccga
    10381 tctttagcct caaaaatagc atcaatctta tcatttggtt ttacccgttt gatgccaagc
    10441 aagtgcaaat cgtggttgcc tagcacggtt ttggcattgt ttgcagagtc tttcacaaaa
    10501 cgtaaacagg ctaaagagtc ctcaccacgg gcgactaaat cccctgttaa aaagagttcg
    10561 tcttgcttgg ggttataatt gactttttct aataaaagct gaaattcgtt aaagcaaccg
    10621 tgtaggtcgc ctacaatata agttgccatt gtttctcctt tgttccccta aagttataaa
    10681 caagcggtta tattttagag aaaatttgca aaatctcatc aaaatataac cgcttgaaat
    10741 ttaaatgata tttcctacga ttgagcgggt ttctaagcgt acttcggtga gtttcacttt
    10801 caccgcttgc ccgatctgat aggctttttc acctttaata taaagtgcaa gttcttcttg
    10861 gcggtattcc atttcctctt tgttgtcgtg caagctggag aatggcacaa aaatttgagc
    10921 accgtttttt atgactttcg cacgcaagcc accacgggaa acatctgcaa tttcgcactc
    10981 aaattcgacc gcttgttcca ccattggggc aaggtaacga gcataaagcc agtcagcaat
    11041 atcacgttct actaaacggt tttgacggcg agcttcttgt aaacggctta aaactgattc
    11101 ttccaccgct tggctttgtt tgccaagtaa cacttgcttg attaaacggt ggtttaccat
    11161 atcgccatat ttacggatgg gtgatgtcca agtggcgtaa tcggtaatgc ctagtccgaa
    11221 atgcggggca acttcggctt taaattcagc gaaggttaaa taacgtcgta aacgcaactc
    11281 taagaatttc tctgggtagt cttgaatatc acgttgcatt tggcaatagc cttctaaggt
    11341 tgctaaacgc tcggcggagt agcgttcggc aagctcatca cgattttcat cgctggctaa
    11401 agtttggagt aaaaatgatt ttaccaattc ataatttttc ggatcaaaac ctgaatgggt
    11461 attaaataca ccggttttgg catgttggct taagaatttt gcacaacaaa tattggcaag
    11521 aatcattgat tcttcgatca tttgattcgc aatacggcga tattcaacgt gaatatcttt
    11581 taccgagcca tcttcattta actcaaaggt ataatcgcct tgttctttga atagcaacgc
    11641 attctcggca cgccattgaa tgcgggcttg agtaaattga tgtagccact caatttgttg
    11701 ttttgtttct tcagactgag gttgccacgc attttccacg ccttcgagat aatcggaaac
    11761 atcgtcgtaa gccaagcgat cttttgattc gacccaagca gaggtaaagg ttgtctcttt
    11821 aatgagattg ccgtttaaat cggtttcaat aaagccaacg agggcagggc gtttttcatt
    11881 cgggacaagc gaacagagat cgtctgagag ttctcgaggt aacattggaa tgttaaagcc
    11941 cggcaaatag ttggtgaaac aacgctggcg agcggctttt tcaatatggg aattttctgg
    12001 gatataagcg gttgggtcag caatcgctac cactaaacgc cagccggttc gcttgccatt
    12061 ttgcttaatt ggttcgatga acagagcatc gtccatatct tgcgtgcttg ggctgtcaat
    12121 agtggtaaag taaagatgag ataaatcttc acgagctaat tcatcgtgta attcatagtt
    12181 tttttcattt gcgaccggtt cacgaggctg ttcgtggcgg gcaagtgtga cccaccaagg
    12241 ggcaaaatta tcagattctt tacagataaa ttgggtaact tgagcaaaga aaaaacggtc
    12301 gtcacgcaat gggtgcgttt ttaattgtgc aaccacccag tcgccatttt ctaatttttc
    12361 agtggctttt ttatgggtat ttgccgggat agtttgctta atgcttgggt gatcaaccgc
    12421 aagttgcaat ttgccctctt tattcaagcg aacttgggca ataaatctat ccagcattgg
    12481 ctcaagtagt gaatcaattt ctacctgttc tttgtcgcca tcacgtttga ctacggcttt
    12541 cactgaatca ccgtgcatta cttttttcat ttccgcaggt ggaataaaat agcttttttt
    12601 atcgcattct aaaaaaccga atgctttgtc tgaggctttg acaacgcctg cgacatattc
    12661 tttgcttgct tcaatttgtt gtttgagttg ggctaaaagg ggattgtttt gaaacataat
    12721 ttctcatttt aaaaataaaa aaaggtgcta tcgttgcacc gaaaaaaaca atggcattat
    12781 aacggatttg aaccaagttc agcaaataaa tgctgttgct ataacttata aaggttggta
    12841 gaatataatc catttaaaaa taatggagac ttttcaatga aaaaattact tgcaattgca
    12901 acagtatttt ctttagcagc ctgttctact caaacacttg aactatctcc tgctaaggaa
    12961 gctcaatatg atcgtgttca acacttcttt gtttcagggc ttgctcaaaa acaagagata
    13021 gatgcagcaa aaatttgtaa tggtgcagat aaggtaggta aggtagaaac tgaaactact
    13081 ttctttaatg gcttgttagg tacaattact tatggtatct attcacctcg ccaaatccgt
    13141 gtatattgta actaatccta gtaatttaag gagttgaaaa tgaaaaaatt gcttgctatg
    13201 tctgtattaa cagccctgct gactacagca tgtacaacac aaactgccca tattggcggc
    13261 aaagttactg ataatattaa accaactaag agtgtaactc agtcgttctt tataggtggg
    13321 attgggcaag aagaaacatt gaatgttgta gaggtttgtg gttccaaaga taaagtgcag
    13381 caggttgaaa cagtactttc aggtggggac attctattag gtcttgtgac tatcggtatt
    13441 tatacacctc gtactgcaaa tgtatattgt aagtaa
//
LOCUS       DANLUA010000055        13327 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLUA010000055 DANLUA010000000
VERSION     DANLUA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13327
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            27..530
                     /locus_tag="PXB44_001138"
     CDS             27..530
                     /locus_tag="PXB44_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL5281625.1"
                     /translation="MNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYM
                     LLIDNGIPIAYCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLL
                     YKKMCQKYPDMIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKN
                     EFNFIKK"
     gene            546..3407
                     /locus_tag="PXB44_001139"
     CDS             546..3407
                     /locus_tag="PXB44_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL5281626.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3482..5608
                     /locus_tag="PXB44_001140"
     CDS             3482..5608
                     /locus_tag="PXB44_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL5281627.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5620..7056
                     /locus_tag="PXB44_001141"
     CDS             5620..7056
                     /locus_tag="PXB44_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL5281628.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7297..8295
                     /locus_tag="PXB44_001142"
                     /pseudo
     CDS             7297..8295
                     /locus_tag="PXB44_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8312..9055
                     /locus_tag="PXB44_001143"
     CDS             8312..9055
                     /locus_tag="PXB44_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281629.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            9210..10268
                     /locus_tag="PXB44_001144"
     CDS             9210..10268
                     /locus_tag="PXB44_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL5281630.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(10389..10931)
                     /locus_tag="PXB44_001145"
     CDS             complement(10389..10931)
                     /locus_tag="PXB44_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5281631.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(11083..12594)
                     /gene="glpK"
                     /locus_tag="PXB44_001146"
     CDS             complement(11083..12594)
                     /gene="glpK"
                     /locus_tag="PXB44_001146"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL5281632.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12598..>13327)
                     /locus_tag="PXB44_001147"
     CDS             complement(12598..>13327)
                     /locus_tag="PXB44_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HDL5281633.1"
                     /translation="LFFGAGCVAAAQVAGANFGLWEISIVWGLGVSMAIYISAGISGA
                     HLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALIYFMYSDLFTAAEAAQGITR
                     GETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTILAIGDDKNGLPNKALAALLI
                     GLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEIALTGGKEIPYFIIPIVAPI
                     CGALFGAWGYKNLIHNNLPANTQE"
BASE COUNT         4041 a         2620 c         2871 g         3795 t
ORIGIN      
        1 tttagaataa ttatcgagtg caaattatga atcaatctta ttttaactta ctaggaaaca
       61 ttacttggct atggatgaac tcctccctcc acaaagaatg gagctgtgaa ctactagcac
      121 gcaatgtgat tcctgcaatt gaaaatgaac aatatatgct acttatagat aacggtattc
      181 cgatcgctta ttgtagttgg gcagatttaa accttgagac tgaggtgaaa tatattaagg
      241 atattaattc gttaacacca gaagaatggc agtctggtga cagacgctgg attattgatt
      301 gggtagcacc attcggacat tctcaattac tttataaaaa aatgtgtcag aaataccctg
      361 atatgatcgt cagatctata cgcttttatc caaagcagaa agaattaggc aaaattgcct
      421 actttaaagg aggtaaatta gataaaaaaa cagcaaaaaa acgttttgat acatatcaag
      481 aagagctggc aacagcactt aaaaatgaat ttaattttat taaaaaatag aaggagacat
      541 cccttatggg aactagactt acaaccctat caaatgggct aaaaaacact ttaacggcaa
      601 ccaaaagtgg cttacataaa gccggtcaat cattaaccca agccggcagt tctttaaaaa
      661 ctggggcaaa aaaaattatc ctctatattc cccaaaatta ccaatatgat actgaacaag
      721 gtaatggttt acaggattta gtcaaagcgg ccgaagagtt ggggattgag gtacaaagag
      781 aagaacgcaa taatattgca acagctcaaa ccagtttagg cacgattcaa accgctattg
      841 gcttaactga gcgtggcatt gtgttatccg ctccacaaat tgataaattg ctacagaaaa
      901 ctaaagcagg ccaagcatta ggttctgccg aaagcattgt acaaaatgca aataaagcca
      961 aaactgtatt atctggcatt caatctattt taggctcagt attggctgga atggatttag
     1021 atgaggcctt acagaataac agcaaccaac atgctcttgc taaagctggc ttggagctaa
     1081 caaattcatt aattgaaaat attgctaatt cagtaaaaac acttgacgaa tttggtgagc
     1141 aaattagtca atttggttca aaactacaaa atatcaaagg cttagggact ttaggagaca
     1201 aactcaaaaa tatcggtgga cttgataaag ctggccttgg tttagatgtt atctcagggc
     1261 tattatcggg cgcaacagct gcacttgtac ttgcagataa aaatgcttca acagctaaaa
     1321 aagtgggtgc gggttttgaa ttggcaaacc aagttgttgg taatattacc aaagccgttt
     1381 cttcttacat tttagcccaa cgtgttgcag caggtttatc ttcaactggg cctgtggctg
     1441 ctttaattgc ttctactgtt tctcttgcga ttagcccatt agcatttgcc ggtattgccg
     1501 ataaatttaa tcatgcaaaa agtttagaga gttatgccga acgctttaaa aaattaggct
     1561 atgacggaga taatttatta gcagaatatc agcggggaac agggactatt gatgcatcgg
     1621 ttactgcaat taataccgca ttggccgcta ttgctggtgg tgtgtctgct gctgcagccg
     1681 gctcggttat tgcttcaccg attgccttat tagtatctgg gattaccggt gtaatttcta
     1741 cgattctgca atattctaaa caagcaatgt ttgagcacgt tgcaaataaa attcataaca
     1801 aaattgtaga atgggaaaaa aataatcacg gtaagaacta ctttgaaaat ggttacgatg
     1861 cccgttatct tgcgaattta caagataata tgaaattctt actgaactta aacaaagagt
     1921 tacaggcaga acgtgtcatc gctattactc agcagcaatg ggataacaac attggtgatt
     1981 tagctggtat tagccgttta ggtgaaaaag tccttagtgg taaagcctat gtggatgcgt
     2041 ttgaagaagg caaacacatt aaagccgata aattagtaca gttggattcg gcaaacggta
     2101 ttattgatgt gagtaattcg ggtaaagcga aaactcagca tatcttattc agaacgccat
     2161 tattgacgcc gggaacagag catcgtgaac gcgtacaaac aggtaaatat gaatatatta
     2221 ccaagctcaa tattaaccgt gtagatagct ggaaaattac agatggtgca gcaagttcta
     2281 cctttgattt aactaacgtt gttcagcgta ttggtattga attagacaat gctggaaatg
     2341 taactaaaac caaagaaaca aaaattattg ccaaacttgg tgaaggtgat gacaacgtat
     2401 ttgttggttc tggtacgacg gaaattgatg gcggtgaagg ttacgaccga gttcactata
     2461 gccgtggaaa ctatggtgct ttaactattg atgcaaccaa agagaccgag caaggtagtt
     2521 ataccgtaaa tcgtttcgta gaaaccggta aagcactaca cgaagtgact tcaacccata
     2581 ccgcattagt gggcaaccgt gaagaaaaaa tagaatatcg tcatagcaat aaccagcacc
     2641 atgccggtta ttacaccaaa gataccttga aagctgttga agaaattatc ggtacatcac
     2701 ataacgatat ctttaaaggt agtaagttca atgatgcctt taacggtggt gatggtgtcg
     2761 atactattga cggtaacgac ggcaatgacc gcttatttgg tggtaaaggc gatgatattc
     2821 tcgatggtgg aaatggtgat gattttatcg atggcggtaa aggcaacgac ctattacacg
     2881 gtggcaaggg cgatgatatt ttcgttcacc gtaaaggcga tggtaatgat attattaccg
     2941 attctgacgg caatgataaa ttatcattct ctgattcgaa cttaaaagat ttaacatttg
     3001 aaaaagttaa acataatctt gtcatcacga atagcaaaaa agagaaagtg accattcaaa
     3061 actggttccg agaggctgat tttgctaaag aagtgcctaa ttataaagca actaaagatg
     3121 agaaaatcga agaaatcatc ggtcaaaatg gcgagcggat cacctcaaag caagttgatg
     3181 atcttatcgc aaaaggtaac ggcaaaatta cccaagatga gctatcaaaa gttgttgata
     3241 actatgaatt gctcaaacat agcaaaaatg tgacaaacag cttagataag ttaatctcat
     3301 ctgtaagtgc atttacctcg tctaatgatt cgagaaatgt attagtggct ccaacttcaa
     3361 tgttggatca aagtttatct tctcttcaat ttgctagagc agcttaattt ttaatgattg
     3421 gcaactctat attgtttcac acattataga gttgccgttt tattttataa aaggagacaa
     3481 tatggaagct aaccatcaaa ggaatgatct tggtttagtt gccctcacta tgttggcaca
     3541 ataccataat atttcgctta atccggaaga aataaaacat aaatttgatc ttgacggaaa
     3601 agggctttct ttaactgctt ggcttttagc tgcaaaatcg ttagcgttga aagcgaaaca
     3661 cattaaaaaa gagatttccc gcttacactt ggtgaattta ccggcattag tttggcaaga
     3721 taacggtaaa cattttttat tggtaaaagt ggataccgat aataaccgct atttaactta
     3781 caatttggaa caagatgctc cacaaattct gtcacaagac gaatttgaag cctgctatca
     3841 agggcagtta attttggtca cgtccagagc ttccgtagta ggtcaattag caaagttcga
     3901 tttcacctgg tttattccgg cggtgatcaa ataccgaaaa atctttctag aaaccttgat
     3961 tgtttcgatc tttttgcaaa tttttgccct aattacaccg ctattcttcc aagttgttat
     4021 ggataaagta ctggtgcatc gaggtttttc aaccttgaat atcattacgg ttgccttagc
     4081 tattgtgatc atctttgaaa ttgtactaag tggtttgaga acctatgttt tttctcatag
     4141 cactagccgt attgatgttg aattaggcgc taaattattt cgacatttat tatcactacc
     4201 catttcttat tttgaaaaca gacgagttgg agatacagtc gctagggtta gagaattaga
     4261 tcaaattcgt aatttcctta ccggacaagc attaacctcg gtgttagatc tcttattctc
     4321 ttttatcttt tttgccgtaa tgtggtatta cagcccaaaa ttaaccttgg taattcttgg
     4381 ttcattgccc tgctatattt tatggtcaat ttttattagt ccgattttaa gacggcgttt
     4441 agatgagaaa tttgcccgaa gtgctgataa ccaagcattc ttagttgagt cggtaacagc
     4501 catcaatatg attaaagcga tggcggttgc tccacaaatg acggatacat gggataaaca
     4561 gctggcaagc tatgtttcat caagtttccg tgtcaccgta ttagcaacca ttgggcaaca
     4621 aggtgtacaa cttattcaaa aaaccgttat ggtgattaac ctttggttag gggcacactt
     4681 agttatttca ggcgatctga gtattgggca attaattgcc tttaatatgc tatcagggca
     4741 agtgattgca ccggtgattc ggctggctca gctctggcaa gatttccaac aagttgggat
     4801 ttccgtcact cgcttaggtg atgttttaaa ctctccaacc gaacaatatc aaggcaaatt
     4861 atcactacca gaaataaaag gcgatatctc atttaaaaat atccgcttta gatataaacc
     4921 agatgcacca actattttaa ataatgtgaa tttagaaatt aggcaaggag aagtgattgg
     4981 gattgttgga cgttccggtt caggcaaaag tactctgact aaattactgc aacgttttta
     5041 tattcctgaa aatgggcagg ttttgattga tggacatgat ctagccttag ctgatccaaa
     5101 ctggctacgc cgtcaaatag gtgtagtgct gcaagataat gtgttattaa accgcagtat
     5161 ccgagaaaat attgcgctat cagatccagg aatgccaatg gagcgagtaa tttatgcagc
     5221 aaaattagca ggggctcacg attttatttc agaattgcgt gaaggttata acaccattgt
     5281 gggtgaacaa ggagcggggc tttcaggcgg gcaacgccaa cggattgcga ttgctcgagc
     5341 tttggtaaac aacccgaaaa tcctgatttt tgatgaggca accagtgccc tcgattacga
     5401 atctgagcat attattatgc aaaatatgca aaaaatatgc caaggcagaa ccgtgatttt
     5461 gattgcacat cgtttatcga ccgtcaaaaa tgcggatcga attattgtga tggaaaaggg
     5521 ggaaattgtt gagcaaggca agcaccacga attactgcaa aacagtaacg gactttattc
     5581 ctacttacac caattacaac ttaattaaga aggaaaacaa tgaaaatatg gcttagtggt
     5641 atttatgaat ttttcctacg ctataaaaac atttgggcag aagtatggaa aattcgtaaa
     5701 gaattagacc acccaaacag aaaaaaagac gaaagtgaat ttttaccggc acatttagaa
     5761 ctgattgaaa ccccggtttc taaaaaacca cgtctaattg cttatttgat tatgctattt
     5821 ttagttgtgg caattgtgct tgccagtgta agcaaagttg aaattgtggc gactgctccc
     5881 ggtaaattaa cttttagtgg cagaagtaaa gaaattaaac cgattgaaaa cgccattgta
     5941 caagaaattt tcgttaaaga tgggcagttt gtggaaaaag ggcaattatt agtcagctta
     6001 actgcattgg gttctgatgc agatatcaaa aagaccatgg cttcactttc tttagctaaa
     6061 ctggagaact atcgctacca aactttgctt actgccattg aaaaagagtc cttgccggtg
     6121 attgatttat ctagaaccga atttaaagat tcatcggaag aagatcgact acgtattaaa
     6181 cacttaattg aggagcaata caccacttgg caaaaacaaa aaacacagaa aactttagcg
     6241 tataagcgta aagaggctga aaaacaaaca atatttgcct atgtccgtaa atatgaaggt
     6301 gcaacacgta ttgaacaaga aaaattaaaa gactttaagg cactttataa acagaagtct
     6361 ttatctaagc acgaacttct tgcgcaagaa aataaattaa ttgaggctca gaatgagcta
     6421 gctgtttatc gctcaaaatt aaatgaatta gaaaatgatc tactcaatgt aaaagaagaa
     6481 cttgaattga tcacgcaatt ctttaaaagc gatgtgttgg aaaaattaaa gcaacatatt
     6541 gaaaatgaac gccaacttcg gctcgagtta gaaaaaaata atcaacgcag acaggcctcg
     6601 atgatcagag caccggtttc cggtacggtt cagcaactga aaattcacac tataggtggt
     6661 gttgttacga ctgctgaaac cttgatgatc attgtgccgg aagacgatgt gttagaggcc
     6721 accgctctgg ttccaaacaa agatatcggc tttgttgcag cagggcagga ggtgattatt
     6781 aaagtggaaa ctttccctta tacacgctat ggttatctaa ctggtcgaat taaacatatt
     6841 agcccggatg cgattgaaca acctaatgta ggcttagttt ttaatgcaac tatagctata
     6901 gataggaaga atctaacatc gcctgatggg cgaaaaattg atttgagttc aggtatgaca
     6961 ataactgctg aaatcaaaac cggtgaacgg agtgtaatga gttatttact cagcccatta
     7021 gaagaatctg tcacagaaag tttaagggaa cgctaatcga accaaaacaa agccataaaa
     7081 gccattttga gcttttatgg ctttattttt tagtccacaa gcggtcaaaa aagcccaatt
     7141 ttttacactt ttataacaaa ttgttctaac taaaaattac taattctttt cttttataag
     7201 acgaatcctc tattttcatt tcattaacat tgaactagaa aaggggattt atgagcctaa
     7261 gcattacgaa tctttctctt ggctaccgca aaaatcagca aaggcttatt tgaaaagcac
     7321 ggtgtgcagg tggaaaaacc ggtgatgttt cgcagctggg ctcagttggt ggaagccttt
     7381 ttaagtggca atgtgaacgt ggtgcatctg ctttcgccta tgagtttgtg ggcgaaatat
     7441 ggagcaaatg ctccggtgaa agcggtaatg tggaatcact tggcaggttc ggctttaacg
     7501 gttcgccctg aaatcaacag tattgccgaa ctctccggca aaacggtagc aattccgttt
     7561 tggtattcaa ttcataatat cgttttgcaa cgattattac gagaaaatgg cttgaaaatc
     7621 accgaaaaag agccgaaaag cggtgaagtg cgattaagtg taatggctcc gtcagatatg
     7681 gtggcagcgt tggcaagtga ggcaatttcc ggctttattg tggcagagcc gttcaatgcg
     7741 ttagcggaag ccaaaggtgt ggggaaggtg ctgcgtttta gtggcgatgt gtggaaagat
     7801 cacgcctgct gtttaaccct aatgcacgaa tacgatattc aacaccgccc ggaatgggtg
     7861 caaaaagtgg tgaatgtctt aaccgaagcc caagtgtcca cgcttgccaa ccgtgcagaa
     7921 accgctcaat tattggctcg gggcagcggc tacacgccgc acgatcaaaa agtgctggaa
     7981 aaggtactgg ctccaaccca agctcagtgg gataaatata tcgaaacagg ggcaattcaa
     8041 aaccctcagt ggcaccaaca gcgaatcggt ttccaaccgt atccgttcga ttcctatatg
     8101 gaagagctgg tcaaaatgct caaacagacc cacattgccg gcaataatgc gtttttacaa
     8161 cagcttgacc cggtagaggc agcgaaagcg attaacgagc cgacctttgt gcgaaaagcg
     8221 attgagcaag gcggttgggt tgaaaccttt aatttacaaa ccggttggac aagaaccgag
     8281 caaattagtg tataagcgat aggaacgaac gatgaaacag aaatatattt acggcttagc
     8341 cggtttatta ctgctacttt taggttggca gcttggcaca gccttgttaa gccaacggtt
     8401 gccgatggcg tcaatgttag ctccggcaaa aacctgggaa agttttactt ggctgatttc
     8461 ctccggcgaa atttttccgc acttatgggc gagtttgcaa cgcattgctg tcgggctact
     8521 cgctgcgtta gtgattggtg taccgctggg cttattgctc ggcttctcca agcaccttga
     8581 aatgctttgc acgccgtcat tccaactact acgtatgatt tcgccacttt cgtggatgcc
     8641 gattatcgtg atgttacttg gtgtaggcga tttgccgatt tattttttac tggcgtttgc
     8701 caccgtttgg tcgttgatta tttccaccac ctcaggggta aaaagcattg atccacaatg
     8761 gttacaactg ggtagagtaa tgtcggctac ccgcaaagaa ctgctgtgca aaattgttgt
     8821 accggctatt accggacacg ttctcaccgg tttacgtgtg gcggttggga ttgcgtgggt
     8881 ggtgctggta ccgtgcgaaa tgctaggcgt gaatcaagga ctcggctatt ttattttaga
     8941 cacccgagac cgattagcct attccgaact gatggcggta attataatta tcggcttaat
     9001 cggctgggcg ttggatagct taatccgctt ggctttagcg aaaggcggca gataaattga
     9061 caagcggtcg aaaaattgca tttttttgca aaaaatcgac cgcttgttta ttcccaaaaa
     9121 gcatattacc ttaatttatt ttatttctag ggcataaaaa agcgatttaa gatagcgaca
     9181 aagtaaacta attatacgag gtaaacacaa tgtcggttct ttcatcttct tttattcaat
     9241 ggcttcagca gaacgccgaa aatttagatc aatctaacga acgagcggat ttactcattc
     9301 cacaaattgc cgcactaggg ctatataaaa tcggcgtgcc ggaaacgctt ggcggcagcg
     9361 gtggcacttt ggttgatgcg atcaaagccg ttaccgaact gggtaatcac tcgctggctg
     9421 cggcatttgt ttcgtgggga catcgtacct ttatcgagca tattatcgcc tcaaaaaatc
     9481 ctattccccg ccaaagctat ttggcggatc tgctaaatgg caatttaacc gcaggaacag
     9541 gtttatccaa tgcgatgaaa tttctctccg gcattgaaga gttgaatgtg aatattcgtg
     9601 aagaaaacgg taaacgctat ttaaatggcc gcctgccttg ggtaaccaat ttacgttcat
     9661 accgctttgt tgctagtttc gttgccggtt ttcaagacga acgcaaaccg ctagtgattg
     9721 caattccctc caccgccaaa ggcttaaccc gcaccaaaga attggaattt atcgcattgc
     9781 aaggcacgaa tactacctct ctcgtatttg agaatgtgga attacaagat gaatggattt
     9841 tggctgatga cgcgcctgag tttttagcag ccactcgccc tgcgttctta ggtttacaat
     9901 ttggtttgcc ctttggcttg gttgaacgta gtttggcgga aatcgaaaaa tcgctccctt
     9961 tccgtgcaat tctcgcctct gagtggcaag ctcaagttga tgccctcaac gatattaaaa
    10021 ctcgtctgtt taatggctta aatgaagaag gttattttgt gcgtaatccg aaagagcttt
    10081 tccaagcgag aattgacatt gtcaatgtcg tcgcccaaac tattctgcta gaactgcaag
    10141 cgagtggcgg acgtggctat ctgaaaaatg atacttcagg cttcagccgc cgttggaaag
    10201 aagcggcatt tctgcccgtt gttacgccaa gtgcggtaca gctgagaatg gtgctggcga
    10261 atagttaagc ggataagaga caaattgttg cctcttttat tttgcaaaaa atagtctaaa
    10321 tataaccgct tgcaataaaa ataaaaaagg atgcattttt tatgcttcct caattttacg
    10381 gctatctatt atgcaaaagg ctgcaattca gggttaattg gaatttcggc aatgttgtta
    10441 acgtagttac ataatgttgc caagctcacg cctaaaatca catcaatcgc attttgttgg
    10501 ttgtaacctg cggcaaaaaa ttcgtttaat tgggcttcgg ttaatttggc tttttgtaac
    10561 attactgcaa gggtgaagcg agccagagtg tctaatttgt tatcgtctat gcgagccgtg
    10621 gcacgtaaag catttaccac ttcttcaggc atttttaatg cttttaaggc gattttagta
    10681 tgacctgcca cgcagaaacc acagccgttg accactgctg cggtgatttg taccacttca
    10741 cgttcttcag cggttaagct gttacgacta ttaataccac ccacagtgcg gtaagtttcc
    10801 agtgctgttg gtgcgttggc taatacgccg atcagatttg ggataaaacc gtttgcttgt
    10861 tgcactgctt ttagggcttc ttgtgccgtt tcaggagcgg attcaatagt gtgaatttga
    10921 aattgagaca taaatgttcc ttatcaggtt gtttattggt gtggctcatc ataaagaaaa
    10981 ggctaattta gaacaaatca aaatcactat ttaatataac taaaagatct taaagggagt
    11041 atagttgcct atactccctt tgtttatgca caggttgcgt ttttactctt ctgcgtcttc
    11101 ttttgcccat tctaatgagc ggcgaacggc ttttttccaa cctttatagc gtttcgcccg
    11161 tttttcttgg tcgccgtccg gtgtgaaggt gcgttcaatt tccgctttgt ctttcagttc
    11221 ttccaaatct ttccagaagc cgacagcaat acccgcaaga taagctgccc ctaaagcagt
    11281 gacttctttc actttcggac gctcaacttt ggtgtctaaa atatcggctt ggaactgcat
    11341 taagaagttg tttgcggttg caccaccgtc cacacgtaac gcttgtagtt tcgctttgga
    11401 gtcagactgc attgcttcta acacatctcg ggtttggtaa gcgatagact ctagggtcgc
    11461 acgcacaatg tggttacggt ttgccccacg ggttaaaccg aaaattgagc ctcgtgcata
    11521 cggatcccaa tatggagcac ctaagccggt aaaggcaggt acaacataaa caccgttaga
    11581 gtcatcttct ttagtggcaa aatattcgct gtcaaagcta tcgtgaacaa ttttcagctc
    11641 atcacgcagc cattgaatag acgcaccggc aataaatact gaaccttcca acgcatattc
    11701 cggctcgcct tttgcgttac acgcaatggt cgttaataaa ccgttttcag attgaatcgc
    11761 agtgttaccg gtgtgcatca acataaaaca gcctgtacca taggtattct tcgcttgtcc
    11821 ttctttcacg cataaatgac cgtagagagc ggcttgttgg tcacctgcaa tacccgcgac
    11881 cggaatacgc acaccgcctt gaccgccgat attagtttgc ccgtaaactt ctgatgaatt
    11941 tttcacttca ggtaacattg agcgtggaat attgagcaat tccagcattt tatcgtccca
    12001 ttgtttggtg tgaatgttaa acaacatagt acgagaagcg ttggtgtaat cggtaacgtg
    12061 tacccgccct tgggttaatt tccacaccag ccaagtatcg accgtaccga acagcaattc
    12121 gccacgttct gctttttctc ttgccccttc tacgttatct aaaatccact tcactttagt
    12181 gccggagaag tacggatcga ctaccaagcc tgtcgtttta cgaatatatt cctcgtgtcc
    12241 gtctgctttt aatttatcgg taatatccga tgtgcgacga cattgccaca caatcgcatt
    12301 ataaacaggt ttaccggtct ctttttccca cacaatcgtg gtttcacgtt ggtttgtgat
    12361 cccgattgcg gcaatgctat ccggcttaat accggctttt gccaccactt catttaaggt
    12421 ggaactttgg cttgaccaaa tttccatcgg gttatgttct acccagcctg cttgcggata
    12481 aatctgggta aattcacgct gagccacttc aaccacattg gcatttttat ctaataaaac
    12541 cgcacgagag ctggttgtac cttggtcgag agcgatgata tattgttttt ccatagatta
    12601 ctcctgagta tttgctggaa gattgttatg aatgaggttt ttataacccc aagcaccgaa
    12661 taatgcaccg cagattgggg cgacaatcgg aatgatgaaa tacggaattt cttttccgcc
    12721 tgttaaagca atttcacccc aaccggcaag ataagcaaag agtttcggac caaaatcacg
    12781 agccgggttc atcgcaaagc cggttaacgg gccggttgct ccaccaatta ccgcaatcaa
    12841 taaaccgatg agtaatgcgg ctaaggcttt attcggcaag ccgtttttat catcgccaat
    12901 cgctaaaata gtggacatca acaccacagt aatcacaaat tcaacgatga aggcggtaac
    12961 caatgtaata ttagggtttg gataggtgga gaacacaccg gcaaagccaa cagtttcgcc
    13021 ccgagtaatg ccttgagcgg cttcggcggc agtaaagaga tcgctataca taaaataaat
    13081 cagtgcgaca gagcaaaacg ctccggcaac ttgggcgatg acatagggca aaattttatg
    13141 cttttcaaag ccgtagaatg cggcaagtgc tatagtgacc gcagggttaa ggtgagcacc
    13201 ggaaataccg gcagaaatgt agattgccat ggaaacacca agcccccaaa cgatggaaat
    13261 ttcccataaa ccgaaattcg caccggctac ctgagcagca gcaacacaac cagcaccaaa
    13321 gaaaaga
//
LOCUS       DANLUA010000056        13262 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLUA010000056 DANLUA010000000
VERSION     DANLUA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..712
                     /locus_tag="PXB44_001148"
     CDS             <1..712
                     /locus_tag="PXB44_001148"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL5281634.1"
                     /translation="PTKEKPFVLGLPTGGTPEKTYKELIKLYQAGEVSFKNVVTFNMD
                     EYVGLPKEHPESYHSFMFKHFFDHVDIPLENINILNGMAEDVNAECERYEEKIRFYGK
                     IHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTEDTRIANSRFFNNDVSQVPKFALTV
                     GVGTLLDAEEVMILVTGHNKALALQACVEGAVNHLWTISCLQLHRRGIVVCDEPATQE
                     LKVKTVKYFKELEKNVAR"
     gene            793..1935
                     /gene="nagA"
                     /locus_tag="PXB44_001149"
     CDS             793..1935
                     /gene="nagA"
                     /locus_tag="PXB44_001149"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL5281635.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2017..2331
                     /locus_tag="PXB44_001150"
     CDS             2017..2331
                     /locus_tag="PXB44_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL5281636.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2410..3600)
                     /locus_tag="PXB44_001151"
     CDS             complement(2410..3600)
                     /locus_tag="PXB44_001151"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL5281637.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(3963..5267)
                     /locus_tag="PXB44_001152"
     CDS             complement(3963..5267)
                     /locus_tag="PXB44_001152"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL5281638.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5277..7103)
                     /gene="oadA"
                     /locus_tag="PXB44_001153"
     CDS             complement(5277..7103)
                     /gene="oadA"
                     /locus_tag="PXB44_001153"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL5281639.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7143..7397)
                     /locus_tag="PXB44_001154"
     CDS             complement(7143..7397)
                     /locus_tag="PXB44_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL5281640.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7587..8774)
                     /locus_tag="PXB44_001155"
     CDS             complement(7587..8774)
                     /locus_tag="PXB44_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5281641.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(9049..9786)
                     /gene="pxpA"
                     /locus_tag="PXB44_001156"
     CDS             complement(9049..9786)
                     /gene="pxpA"
                     /locus_tag="PXB44_001156"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL5281642.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(9773..10702)
                     /locus_tag="PXB44_001157"
     CDS             complement(9773..10702)
                     /locus_tag="PXB44_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL5281643.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(10699..11346)
                     /gene="pxpB"
                     /locus_tag="PXB44_001158"
     CDS             complement(10699..11346)
                     /gene="pxpB"
                     /locus_tag="PXB44_001158"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL5281644.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(11440..12264)
                     /locus_tag="PXB44_001159"
     CDS             complement(11440..12264)
                     /locus_tag="PXB44_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL5281645.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(12398..13222)
                     /locus_tag="PXB44_001160"
     CDS             complement(12398..13222)
                     /locus_tag="PXB44_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281646.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         3609 a         2966 c         2989 g         3698 t
ORIGIN      
        1 accaaccaaa gaaaaacctt ttgtgcttgg cttaccaacc ggcggtacgc ctgaaaaaac
       61 ctataaagaa ttaattaaat tatatcaagc tggtgaagtg agctttaaaa atgtggtgac
      121 tttcaatatg gacgaatacg tcggcttacc taaggaacac cctgaaagct accacagttt
      181 tatgttcaag cacttttttg accacgttga tattccgctt gagaatatca atatcctcaa
      241 cggtatggcg gaagacgtga atgcagaatg cgaacgttat gaagaaaaaa tccgttttta
      301 tggtaaaatc catctgttta tgggtggggt cggtgtggat gggcatattg cgtttaatga
      361 gcctgcatct tcgctttctt cccgcactcg aattaaaacg cttaccgaag atactcgcat
      421 tgcaaactca cgtttcttta acaatgacgt aagccaagtg ccgaaatttg ctttaaccgt
      481 tggggttggt acgttgcttg atgccgaaga agtgatgatc ttagtgaccg gccataacaa
      541 agccttagca ttacaggcgt gtgtggaagg ggcggtaaat catctttgga ccatcagttg
      601 cttacaatta catcgccgtg gtatagtggt gtgtgatgag ccggcaaccc aagagctgaa
      661 agttaaaaca gttaagtatt tcaaagagtt agagaaaaac gtagctcgct aattctgatt
      721 ttacaagcgg ttaattttgt aatatttttt acaaaaacga ccgcctgtta gcatttaaag
      781 agaagagaga aaatgaacca ttatgcctta acaaactgtg tgatttacac cggtgaacaa
      841 gtgctttacc aacacgcatt agtggtgaaa gaagataaaa tcgactccat tgttttggaa
      901 gaaaatttac cgctggatct gcaaaaaatc gacctaaaag gagcgaatct ttcagccggt
      961 tttattgact tgcaactgaa cggctgcggt ggggtgatgt ttaatgaaga aatcactacc
     1021 cgcactttag aaattatgca ggaaaccaat ctgcgttcag gcacaaccag ctatttaccg
     1081 acttttatta cctcgcctga tgagggagtg aaacaagcgg tggcggtaat gcgtgagtat
     1141 ttgtcgaaac acaaaaacca agcactcggc ttgcatttgg aaggacctta tctaagtgtg
     1201 gcaaagaaag gagtacaccg tccggaattt atccgtgaaa tcagccctga aatgttagat
     1261 ttcttatgcg ataacggcga tgtaattacg aaaatcacga ttgcagcaga aaacccgacc
     1321 gcagaccata tcaaacgctt taatgatgca ggaattattg tatctatcgg gcattccaat
     1381 gccacttttg ctcaaaccaa acagcgaatt gccgaagggg cagcgtttgc tacgcattta
     1441 cacaatgcaa tgtcgccggt cagcaacggg cgtgaaggcg gtgtagtagg ggcggtgttg
     1501 gatagcgata tttacaccgg cattatttgc gatggcttgc acgttgaatg gggcaacatt
     1561 cgcattgcca aaaaagccaa aggcgataaa ttagtgattg tcagcgatgc tgttgctgcc
     1621 gcaggaagtg atattgagtc gtttattttt gtcggtaaaa aagtcacggt gaaagatggc
     1681 aaatgctacg atgaattcgg agcattgggc ggtgcggcaa tcacgatgat cgaatccatt
     1741 cgcaacgtag tgcaacattg cgatattagc ctgaatgaag caatccgaat gagtaccctt
     1801 tacccggcaa aagcgattgg ggtagaggaa acactcggct cacttgaagc cggcaaaatt
     1861 gccaaccttg cgattttcga taatcaattt gaagtaaaag cgactgctgt aaacgggcaa
     1921 ttacagtggc attaattttg ctacaaattc taagcataac agcatataat ccaaaggcag
     1981 gcattgtgtc tgccttttat ttatagggag aataacatgc aatttaaaga ttacttagcc
     2041 acttttccaa ctattgatca tctagccggc ttaaacgtga taaatagcgg tgaagtgatt
     2101 caacatattc ctgcgattga aggtaaatta ggttcactaa aactttacaa tgcactgttt
     2161 gcgaaatttg atgggaaatt aaccgctagt gcagcaaaac aagggttgga atggtttgcc
     2221 gaacacgttg aagatgcgaa aaataatgtg ggtaagcacc cgaatattga tctattattt
     2281 agggtaattg agcaagattt atctttaagt ttagagccga ttgttaaata agattgcact
     2341 agtgaaaaat aacaagcggt cattatctgc aaaatttttg caaataatga ctgcttgtgt
     2401 ttaagaggct tatttcacgg taatgcgtgc aaatttgcgt ttgccgactt ggtaaacaaa
     2461 agtgcctttt tgagcagttt gtttcacatc ttccactttt tcaccatcaa ttttaacccc
     2521 gccttgttgg gctgagcgaa ttgcctctga agtggacggt actaagccgg cttctttcag
     2581 tagagccgct aggccgattt caccctcaaa ggtaaattca ggcacttcat caggaattgc
     2641 ccctttttgg aaacggttga taaactcttg ctctgctgca tctgctgccg cttcatcgtg
     2701 gaaacgagcg ataatttctt ttgccagtag aattttcaca tcacgcgggt ttttgccatt
     2761 ttcaacatcc gcttttaatt gtgcaatttc gtctaatgga cggaatgaga gcaggttgta
     2821 ccaatcccac attaaatcgt ctgaaatcga cataattttg ccgaacattt cgcttggagc
     2881 ttcagttaca ccaatgtagt tgccgagaga tttcgacatt tttttctcgc cgtctaagcc
     2941 gacaagtagt gggagtgtca ttgccacttg tggtttttga ccggctgatt tttgtaactc
     3001 acgtccaact aacaagttaa aggtttggtc tgtaccacct aattccacgt cagctttcag
     3061 ttctaccgag tcgtgacctt gtagtagcgg ataaataaat tcgtggattg cgatcggttg
     3121 ttggttcgtg aaacggtttt tgaaatcttc acgttctaac attcgagcaa cggtgtagtt
     3181 ggaggctaag cggatcatac cttccgtacc taacttgcct aaccattcgg agttgaatac
     3241 gatcttggtt ttttgtggat ctaaaatttt gaagatctgc tctttatagg tttcggcatt
     3301 gcgtaacacg tcttcacggc taagtggcgg acgggtagag ttttttcctg atggatcgcc
     3361 taccataccg gtgaaatcac cgattaagaa atagacttcg tgtccgaatt gttggaattg
     3421 acgaagtttg ttcagcacaa cagtatgacc taagtgaata tccggtgcag ttgggtctgc
     3481 ccctaattta atacgcaatg ggcggttttc ttttaatttg gtgattaaat cttcttctga
     3541 ataaatattt tccacgccac gtttaagttc ggcaaggact gattcgatag cttgagtcat
     3601 ttatcttcct tttagtcttg aatataaaaa atggatctat tatagcggta tgaattgaaa
     3661 ttgataggct ataggcgaaa aaaaacgccg tattttgtgg gggaatacgg cgtaaagagt
     3721 tggagtgatt acctattatt tttgaagtga tttaatgata atgattatca tctgttaagt
     3781 caatactttt tttgaaaatt ttcaataaga attattctca tcaattaagt aagatggata
     3841 ctacggcaat tagtgaagaa tgtacagcgg aattttcgta ttgtaagagt gttggtaagc
     3901 ggtattttta gaaaaaacct ccgagagcgg ttatttttcg gaggtttttt gcaaatgagg
     3961 atttacatcg ttgaaatgta tttcaacatc acccccgctg caatcgcaga gccaatcacg
     4021 ccggcaacgt ttgggcccat tgcgtgcatt aacaagaagt tttgtttatc gtactcttgt
     4081 ccgactttgt ttgatacccg tgcagccatc ggcacggcag aaacacccgc tgagccgata
     4141 agtgggttga ttttgtgctg actaaagcgg ttcattattt tcgccataat cacaccgctt
     4201 gcagtgccga tcccaaaggc aatcacgccc aatactaaaa tgcctagtgt ttgcggttgt
     4261 aagaatttgt ctgcgaccaa ttttgccccc accgataacc ctaagaaaat cgtcacgata
     4321 ttgattaacg cattttgggc ggtgtcgttt aagcgttcta ctacgccact ggttcgcagt
     4381 aagttgccaa agcagaacat tcccaataac ggtgcagcat ccggcagtaa gagtgccact
     4441 aataatagca acacgatagg gaaaataatt ttctcacggt tgcttacatt acggagctga
     4501 accattcgga ttttacgttc ttgctcggtg gttaaggctt tcataatcgg cggctgaatc
     4561 agtggcacta acgccatata agaataagcc gctaccgcaa tcgcacctaa taattcaggg
     4621 gcaagtttgc tggcaagata aatcgcggtc gggccatctg ccccgccgat aatcccaatc
     4681 gcagcggctt gtggcaatgt gaagtcgata atgcctaacc agtttagccc taatgcaccc
     4741 agtacggtag caaaaatccc aaattgagcc gcagccccaa gcattaacat tcgagggttg
     4801 gcaagtaaag ggccgaaatc agtcatcgcc ccgacaccca taaagataat aagcggagca
     4861 acgccgtagc cgatagcaac tttatagaat aatgccaaca caccggcggt atagcccata
     4921 tcgccagcca ttacttcaag ctcattttga acagagggag cagccagtgc gatggcatct
     4981 ttaatcgaat gcagatcaag gcttgtacta tttaccttag cagcaataat tgccagttgc
     5041 tcggcagaac cgctgtggag taaattgtct aatgccgtca ttgccatacc tgcttccgga
     5101 atgtttgaca tcaaaccacc aaagccaata ggcagtagta aaagcggttc gaattttcgg
     5161 gcgatggcaa gccaaagcaa cactaaactg atgacaatca taatcgcttg ccccagttct
     5221 aaatgcatta gccccatacc tttaaaaagt gcaataatac tttccatacc ggactcctag
     5281 gcaatactca ttaaagtatc atttaccgcc acaacatcgc cttgtttgac attaatgcct
     5341 tgaatcgtgc cggattttgc cgcacaaatt tgggtttcca ttttcattgc ttcaagaatc
     5401 agtaacacat cgccttcagc aacttgttgc ccctctttca cttccacttt caaaatgttc
     5461 cctgccattg gagcattcac cggttcggca ttggctgata gcgtatttgc agattttggt
     5521 gcagaatcga ccgcttgcgg agccggtgtt gctggtgttg gagcgatatt ggttatatcg
     5581 ccaccttcac tcaccttcac cacaaaggct ttgccttcaa gctcaacggt ataaatggct
     5641 gaaccggatt gtgtttgggc ttttggtgca gaagcagcgg aggcttgtgc cggttttgct
     5701 tcatcagcac ttggcactgg ctcaaaggca gacggattat tgcggttttc caaatatttc
     5761 cagcccactt gttggaagag tgccacaatc aatacatcat caatcatgtt ttcagatagc
     5821 gtaatgcctt tttcttttgc acttgcagtc acttctgcca ctaatttttc cagttccggc
     5881 ggaagatgat cggctgggcg gtcggtaatc ggctctgctc cctcaagcac tctgtcttgc
     5941 aaggctttgt ttaccggtgc cggtgtgcga ccatattcgc cttttaaaat acctgcagtt
     6001 tctttggcga tagttttata acgttcgccc attaatacgt tcatcaccgc ttgtgtgccg
     6061 acaatttgtg aggttggtgt caccaacgga atattgccta aatcttcacg cacacgagga
     6121 atttcttgta aaaccaagtc taatttatcc gacgcatttt gttgcttgag ttggctttcg
     6181 aggttggtca gcatgccacc cggcacttgg gcaaccaaaa tacggctatc tataccacgg
     6241 agctgtcctt caaattttgc atatttttta cgcacgttac ggaagtaggc agagattttc
     6301 tccagagctg gaatgctcaa gccggtttca tacggcgtgc cttgaagtgt ggcgacaata
     6361 gactcggttg ccgggtgacc gtaagtgccg gacattgatg aaatggaggt atcgacacca
     6421 tcgacaccgg cttcaatcgc tttgagcaac gccatttcag ccataccggt ggttgaatgg
     6481 caatggagat gtagctcaac gtcgtagcgt tttttaattt cgctgactaa ttcataggct
     6541 gccattgggg ttaaaatgcc cgacatatct ttgatcacca acgaatccac gccaatttcc
     6601 aataattgct cggttacatc caaccacgtt tgtagggtat gaacagggct ggtggtgtaa
     6661 ctcaaagtac cttgtgcgtg cccgccgtgt tttttgaccg cttgtaatgc ggcttgcata
     6721 ttgcgagggt cgttgagggc atcaaataca cggaacacac tcataccgtt tgccaccgaa
     6781 cgctcgacaa atttatctac cacatcgtcg gcataatgac gataacccaa taagttttgc
     6841 ccacgtagca gcatttgcat tggggttttc ggcattgctt ttttcagctc acgtaaacgc
     6901 acccacggat cttcgcctaa aaaacgaata caactgtcaa aagttgcccc gccccaagtt
     6961 tctaacgacc aatagccgat attatccagc tcggaggcaa tcggtaacat atcgtctaag
     7021 cgtaaacgag tcgcaaaaag ggattggtgt gcatcacgca aaaccacatc ggtaatcgca
     7081 atttttttcg gttgagttgt cataatcaaa ttccttctaa ttattttatt tttgaaatct
     7141 tgttaatgtt gcccgttaat tcgacgatga tgagcaatcg cagcggcaat cacagggcgt
     7201 aaacgctcta aatcatctgg gggagattga ggcacagttt tagaggctgg aatagaaaca
     7261 ggttcgggga aatattgatt gataatcttg gaaataaagc tgatggcata aattaaaacc
     7321 agcaggaaca gcataacaaa tcccattccg gctaccatta aatttatacc ttccgcaaag
     7381 agttctgcag gtgccataat ttatccttaa aatgtagaaa tgatgaagaa aatgtagaaa
     7441 aaattcagat ttatttcaaa ttatttagcc tcaaaatgag tggtagatca caaactttaa
     7501 acgcaagcgg tcagatttta caaatttttt acaagagtag ttaaaatttg caaaacctga
     7561 ccttaggtta gcttgaagct aagtcgttat gccattagcc ccgaaatata ttttgaaagg
     7621 gtcattgcac ttagcaccgc cattagaatc accacaatcg cccctgaaac ggtcatccac
     7681 acagggtgtt tgtagtcgcc cacaatcttg gttttgtgag ctgccagcag aattaacccg
     7741 agggaaatcg gtagaattaa gccgttaagc gtccctacaa acaccaagac ttgtgccggt
     7801 ctgccgatgg ttgccagcac ggcggttgaa accacaataa aggcgacaat gaaacagttt
     7861 ttgtgttttt cgataaatgg cgagaatgaa gtaatgaagg aaaccgaggt ataagccgcc
     7921 ccaatcacag aggtaatgga ggctgcccaa atcaccacgc cgaagatcat caaaccgaca
     7981 tcgccggcaa cgtgggcaaa tggggtagcc gcagggtttt tcggatcaag ttgcacgccc
     8041 attgacacca cgcccagcac cgctaaaaac agtaccacac gcataattga ggcgattaaa
     8101 atggctgaca ccgagctttt gctcacctct tttaaattct ctttgccttt aatgcctgca
     8161 tcaagcaaac ggtgcgcacc agcaaaggtg atgtatccac caacagttcc gcccactagc
     8221 gtgacaatcg caatcacgtc taatttttcc ggcacaaagg tatgcaccac cgcatcgtcg
     8281 attggcagat tggcttttac ggcaacgaag atggttaaag caatcatcac aaagcccata
     8341 atttgtgcaa atctgtccat cactttacca gcttcatgga acaggaacac gcccacggca
     8401 atcgcaccgc tgaccaccgc acccatttca ggggaaagtc cggtgagaat gtttagccct
     8461 aaacctgcac cgcccacgtt gccgatatta aaggctaaac cgcccatcac gataagcaga
     8521 gccagaaagt aacctgcccc cggcaatacg gcattggcga tgtcttgagc tttcttttcc
     8581 gatactgcta caattcgcca aatattcagt tgagcaccga tatcaagcaa gatcgagagt
     8641 aagatcacaa agccgaagct ggcaagtagg gtttgggtga aggtggctgt ttgggttaag
     8701 aaccccggcc cgatggcgga ggtcgccatt aaaaatgctg ccccgagtag ggcgctacgg
     8761 ttgttgtttg acattgtgtt ggttcctttt ttgttgttaa tgagtcatta ttcgtggatt
     8821 tatcatggta atattcccct ctttagcaaa gaggggttag gggagatttg gcagtagaac
     8881 tcatcgaagt gagagagtta ataatgctca atttaacctc ttgaaattac ttattttctc
     8941 cgtaaatcgc ttctgtcaaa tctcccccag cccctctttt ctaaagaggg gagttcgatc
     9001 tccaagagtt ttatttctgg ttttccgtcc attttgacta aaacaggatc aggctttcac
     9061 caaaatcccc tttgcctgta actctttttt aattcgctcg gcaaatacca cggcgtgttc
     9121 gccgtcaccg tgtaagcaaa tgctgtcggc tttaatcgcc acattaccac catcaacggt
     9181 tggtacagtg ccgtctaaca ccatttgcaa gacttggctg atcgcttcat catcgctttc
     9241 taccattgca tcatcacggg tgcgaggcac gagcgagccg tcagctaaat agtggcggtc
     9301 ggcaaagact tcggaaatgg tttccaaacc gtgctgctcg gcaatgctca acatcaggct
     9361 gccggataag cccatcagtt tgaggttggg atcgaactgt ttgaccgtat tggcgataag
     9421 ggtggcaagg tgagggtctt tggcggccat attgtataaa gcgccgtgcg gtttaacgta
     9481 gctcatcggc atattttccg catcacataa ggctttggcg gctcccagtt ggtagagcag
     9541 acaggcttta agttcatcat cgggcagttg catattggta cgcccaaagt tttccctatc
     9601 tggaaaactc gggtgagcgc caactcgcac gctgttttcc ttcgcccagt taatcgcttt
     9661 acgcatttca ttgtaatcac cggcgtgcaa gccacagcag acattcgccg aagtaacgag
     9721 ctgtaataat gaatgatcgt tgccacaacc ttcggcaaga tcgacattta aatcaacgtg
     9781 attcattcgc ttttctccga attgtttcca aataattttg atctttttga taaatttcga
     9841 ccgcttgttc acggcttact tgctcaaatt gcaccgttga gccgaacgga ttttgtgcca
     9901 accgcccgag atcggcctga ataatgcagg caattttcgg ataaccaccg gtggtttggg
     9961 catctgccat cagcacaatc ggctggccgt ccggcggcac ttgcaccgta ccactcgggg
    10021 ctgcgtagga gagcatttca agggaagcgg tcaattccaa cgctttttct gcaaaacggt
    10081 agcccatacg gttgctgttg ttttgcagtt gccacccttg ttgccaaaag cgttcacggc
    10141 tatctgcggt aaaggcttca tattcgctcg aagcggttgc ccgaatgcgt gaggtgaaat
    10201 caatcggctc aatcgacatc aaagaaagtc ggctgtctct gcggcctgtg gcgatgttat
    10261 cgccggcttt taacattctg ccttgaaaac cgccaaattg agctttgaga gcggtacttg
    10321 ccgaacctaa cacctgcggc accagaaaac cgcctgccac gcataaatag gcgtaattgc
    10381 cgagaacaat ccgttttatc gtcagcgttt gacgggcgtt ggcagtgtag cggtaatagg
    10441 aaaaaaccgg cgtgccgtcc agctccgcct ccaccaatgc accggtgagg caaaatggcg
    10501 tgtcgcaatc aaaggtaatc cgcattccgc caagggtaag ttcaatcgca ggtgcattat
    10561 ttgcattttt taacaataaa ttacccgctt gtaaggcaag gctgtccatc gcaccgccgt
    10621 ggctcacgcc taaatggcga taaccataac gccctaaatc ttgaatgtgg gcaaaaccct
    10681 gtattatttc gatataaatc atagcgttat actctccgcc acaaatctaa cattatcgcc
    10741 ggctgtaagt agcgttggcg gattgtgttg aatatcaaac aacggtaact cggttcgtcc
    10801 taacagttgc cagccacctg gtgaggtaaa gggataaatg cctgtttgcc tgccgccgat
    10861 gccgaccgaa ccggcaggga ctttggtgcg aggcacgttt cggcgtgggg tgtgtaaatt
    10921 ttgcggtaag ccgttgaggt aaggaaagcc gggttgaaag ccgatcataa acacggtgta
    10981 agtcggttcg gtatgacggc ggataatctc ttcgggagtg gtattgtgga attgggcgac
    11041 ttcaaacaga tcttcgccaa attcaccgcc gtaatggaca gggatttcaa tcaggcgtgg
    11101 ggattgtgcg gcttgggctt ggtcgttttc ccagagttgt tgaaccgctt ggataaggcg
    11161 tgtgctatcg gtgccaatgg cgtgaaatag ggtaaaattg ttcattccta ccaccacttc
    11221 caacacgcca tctaaggctc ttgcctgttc tgccacccgc caaatctgtt gctgtttggc
    11281 aagctctgca ggcggttcgg ctaagcagag cagggaatgt tcgctggtat gatgaatcgt
    11341 cagcataatt gccctcaaat tgttacaaaa aggtaacaac tttttacgct gatatatcca
    11401 gactgtcaat tttattttgt tgaaaatgtg aaatagctct taattttcag aaatttgatc
    11461 atttcttatt cgccaaaacg ttgcaaatct gggcttgcct gattctgtga cacctcgata
    11521 tttataggta atgattgagc caatcggtgg cggattttct cgttctttat ctttaaaacc
    11581 gctgccaatc cgaaaacgtc cacgttgatt ttcacaagtg attgccccga gtttatcggt
    11641 atatttccct ttgcctttat gatgggcaac cacagtgcat tcctcatcta aaaccggttt
    11701 gattttcaaa atttgggcag aacgaccttg aatatagcct gcattcgggt tgcgaacgat
    11761 aaccccttcg ccaccttgtt ccagcacaga ttggtaaaat tgttgaaggt gggctttatc
    11821 tttaatcgga atttgctcaa taatagcgat atggagcgtt ggatgttgct tcagataatt
    11881 ctctaaggtc gccagccgtt caatcaaatt cccttgtgca tttggcacat cgaacacctg
    11941 taatttcagc ttgtgccaac ctttgggctg agcggaacgc acaatgcttg aaatttcctc
    12001 aaatttgccc cgttcgctaa acagttcgcc gtcaatcgca aaaggaggga aatcttgaat
    12061 aaaatagtcg ggtggattga gcggataacc ttggcggctg atgagctgtt gaccgtccca
    12121 aaagcctcgc acgccatcga gtttttctga cattacccag ccactgatgt cttgatcttg
    12181 atattgctca acgagcatta actcgggcga tttggcaaaa atagggttac caatgaagag
    12241 taggaataag agcagttttt tcatagtctc tccgaaatag tgcctccgaa tatgcaaggc
    12301 actataaagg aaagcgtgca aacagtcaga aatgaatctc aatttttcga cgcagatcgc
    12361 aaaattttcc gaaaaactga tcgcttgagt gagaaattta acgtactgtt tgaattaaag
    12421 cagaaatatc ctcgctaaag gtttccaact tcgccatttc ttcaatttgg ctatgagaat
    12481 atttcttcac attgtaaacg accaccatac ttacatcacc tacttgcaat aaattacttt
    12541 cgccaaaagg cgtgtagcgt gttgccccaa tgctgataat cgctttagtt gggtagtttg
    12601 cctgttttaa catttcggaa atgttgttca tcggaccttc atcgggctga ttattcattc
    12661 tctctacaat ccaatcgagc aatttttgat ggaaatagct gtactccacc gcagggctgt
    12721 cgatgccgta ggtattcagc tcgccattac gtttatggaa acaagcgatg ttgtaattat
    12781 ccagcgtgcc gcctttgttg aagttatcta acggaagatg aattgccgaa agccctttag
    12841 acgaagcccc ccagtttttc ttttcacaga ttttattcgc attcggacgg cggatagagc
    12901 agtcattgta agccgcaaaa tgggttggcg tgagcgaaac cacttgctga tcttggtagc
    12961 caatttcaca aattaacgcc acttcaggct caatttgcag attatcagca tcactcggga
    13021 aattaatcgc agtatttgaa agcggatagg tggataaaaa ctgataatcc cccaaatttt
    13081 ccgccggcac ataaaacggg aaaatcgcct ttggctgcac tgcttccact gttttcactt
    13141 tcgcaaaatc cgcctcctct ccggcttgtt ctaaatgtcc agcaaaattg ccggccacgc
    13201 ctaaaccgat aaagtgttcc ataatctact ccttaattgg taaaagttgg ataaaatttt
    13261 ac
//
LOCUS       DANLUA010000057        13261 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLUA010000057 DANLUA010000000
VERSION     DANLUA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(97..789)
                     /locus_tag="PXB44_001161"
     CDS             complement(97..789)
                     /locus_tag="PXB44_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5281647.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKGVLSSKSVTPRYKRFLKNAI
                     RQSACPDILAEP"
     gene            complement(786..1211)
                     /locus_tag="PXB44_001162"
     CDS             complement(786..1211)
                     /locus_tag="PXB44_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281648.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1218..1370)
                     /locus_tag="PXB44_001163"
     CDS             complement(1218..1370)
                     /locus_tag="PXB44_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281649.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
     gene            complement(1531..2217)
                     /locus_tag="PXB44_001164"
     CDS             complement(1531..2217)
                     /locus_tag="PXB44_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281650.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     SFNFDDDIPF"
     gene            complement(2612..2935)
                     /locus_tag="PXB44_001165"
     CDS             complement(2612..2935)
                     /locus_tag="PXB44_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5281651.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            complement(3048..3266)
                     /locus_tag="PXB44_001166"
     CDS             complement(3048..3266)
                     /locus_tag="PXB44_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL5281652.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            complement(3541..4767)
                     /locus_tag="PXB44_001167"
     CDS             complement(3541..4767)
                     /locus_tag="PXB44_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5281653.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            complement(5042..5408)
                     /gene="ssrA"
                     /locus_tag="PXB44_001168"
     tmRNA           complement(5042..5408)
                     /gene="ssrA"
                     /locus_tag="PXB44_001168"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5574..6533
                     /gene="rpoH"
                     /locus_tag="PXB44_001169"
     CDS             5574..6533
                     /gene="rpoH"
                     /locus_tag="PXB44_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL5281654.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            6699..6992
                     /locus_tag="PXB44_001170"
     CDS             6699..6992
                     /locus_tag="PXB44_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL5281655.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(7010..7312)
                     /locus_tag="PXB44_001171"
     CDS             complement(7010..7312)
                     /locus_tag="PXB44_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5281656.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(7284..7574)
                     /locus_tag="PXB44_001172"
     CDS             complement(7284..7574)
                     /locus_tag="PXB44_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5281657.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            complement(7712..8251)
                     /locus_tag="PXB44_001173"
     CDS             complement(7712..8251)
                     /locus_tag="PXB44_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL5281658.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
     gene            8322..8999
                     /locus_tag="PXB44_001174"
     CDS             8322..8999
                     /locus_tag="PXB44_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL5281659.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            9184..11031
                     /gene="typA"
                     /locus_tag="PXB44_001175"
     CDS             9184..11031
                     /gene="typA"
                     /locus_tag="PXB44_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL5281660.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(11080..11517)
                     /gene="rimI"
                     /locus_tag="PXB44_001176"
     CDS             complement(11080..11517)
                     /gene="rimI"
                     /locus_tag="PXB44_001176"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL5281661.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(11510..11932)
                     /locus_tag="PXB44_001177"
     CDS             complement(11510..11932)
                     /locus_tag="PXB44_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL5281662.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            12062..13051
                     /gene="rsmC"
                     /locus_tag="PXB44_001178"
     CDS             12062..13051
                     /gene="rsmC"
                     /locus_tag="PXB44_001178"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL5281663.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
BASE COUNT         3694 a         2682 c         2817 g         4068 t
ORIGIN      
        1 tgatttttac ccactgttag ccacttttag aggggaaata ttagcggttt tggtagtggt
       61 taaagggcaa aaaaggcctt aattctcatc aacggcttaa ggttcggcta gaatatcagg
      121 gcaagcactt tggcggattg cattttttag gaagcgttta tatctaggcg ttactgattt
      181 tgacgatagt acgcctttta gtatttgttg ataggcttca tcattaatta gggctttttg
      241 aatgaaataa ccggcttcta tatttgcctt aatctcatct aacgaatcga ttcttatatg
      301 gcggtagaaa ataaagctgt cccgtgtgat aaattcgtgt gttcctgcct ttaaaataca
      361 agtttcatca agtgctttcc cgtctttaat gcttgagcag tttacactta aaatcatatc
      421 tcctttaccg gtttcaccat caaagcacgg atcggtaacc tgcttatcca gccctagcaa
      481 cttcgctttg cccattgtgg cttgagtcat tgcgttaggg ttttctttct ctctcgccag
      541 tatacgagcc tcttccagct cctttaaaag gtcatcaacg gttaatttat ggcgttctgc
      601 gtgttcggct ttcagttcct caatccttgc cgtaatcttg ccgttatcca ttagctcttt
      661 agcttttcgg tggacggttt cggacttcat atcttcggtt tcataggctt ggcggtaggc
      721 ttcgctagca ttgccggttt caatataagc aaagcaaaaa gcctcttgtt ttggtgtgag
      781 ttcggtcatt ctttcccctc cgccattcgc ttttcagcaa aggcataatc gattttttct
      841 cggtattctt tgctcatctg ctcccgtgta atttcgttgc cgtgtcggtc aataaatggg
      901 tttgttcgtt gtgggtgggg gagcttgttg atttcactca ataagtcatc attcactaat
      961 agcggcgtgg cggttagctg ttgatgatag gcaataatcg ctagagcctc taaaaagccc
     1021 gtagcccctg ccatttcaat attgatatga tcttcaaggt caagagaaag cacaccgata
     1081 aaaaacggtc tgccgttatc gtggtgcagt cgttcaattc ggatatgcgg aaaatctaaa
     1141 aagctcaagg ttgctttgat aggttcattc attaaagcag taatacccgc taaattaagc
     1201 tcgtgtttca ttttggttta ttcctttatt ctttccgcca cttttaacag ttggctaagt
     1261 tgttttctta aaatgccgag tgtgattgaa tcggtttcgc ttggcattgc gttatgttgt
     1321 tgttccagct tcgctattcg gttgcctagc acaattaaaa gctcgggcaa tgggtaagct
     1381 cggtttcaga atagaggctt aaaaacgtga atagtctgct aaataatcag gtgttatctc
     1441 agaaaaatcg agctttttcc ctagtgctgc ttctaattct tgtttattta aaagtaaatt
     1501 ttggaaatca tttaaattca ttgctgccac ctagaacgga atatcatcat caaaattaaa
     1561 gctgcttgat tgtttttcat atccgttatt aattgctaca aaatcaaagg ctgcttttaa
     1621 attagtatta tctttatcaa tgcttggact tattccgcca tattgtcgat aaagctgtat
     1681 atttttatct ttgagtaagc ggtttaaatc gttagctgtt tcactaagac ttgagtaatg
     1741 tttacttgcc cattctatga agtcataaac agaaatataa tcattttgaa tttggctaat
     1801 ctctgattgt gtttgcttgg tggttaattt atctaacaag gttggcgatt gtgttttaat
     1861 cagctgtgtt atttctcttg ctgttacgta ataagcattt aaatgttcaa catcaataga
     1921 attttgagca ataacatcac ttaaaacgcc tttaggctga tcgaaagcgt ctttgaaatg
     1981 tagtgttttg ggctcaatag catagctttc accatcttca aagagagtgc aatttacaaa
     2041 agcattcgta tatattccat tttgctgaaa ggaaaaaatg aggcttttca atttatccgc
     2101 tatatgtgaa aaaggtttat ttgtatagtt tgataattct tgtatcagtt cttgaaagct
     2161 gattttgggc tcaatacgat tttcaagcct agaaataaat tgactgtgat ctgccataag
     2221 cgccccttta acgtatccct ttactgtgag agcgtggaaa gagaaataaa gggctttctc
     2281 ctttcgtgcc ggttaattac tccgccacta tccacgctaa tttgaataaa tccgtatagt
     2341 tacggattga acttgatagg ttgtattttg ctaattattc agataaatta caagtaataa
     2401 gctgtgtttt attacagtaa atcaaaatag gctaaagatg ggtttccgga cgctataaaa
     2461 aagagcattc aagcggtcat atttcattaa ggttttgcga atagtgcgga attttcggag
     2521 cagtacgcac gcaaggagca aataaaaagc cgtcaatatc catcaacggc tatttaaaag
     2581 tgaggttttg acaggtagca agtgataagt tttagccatt tttgacttgt ggaataagca
     2641 ctaaattgaa tgcccctaga attttttgaa tagtgtcaaa gcgtggtttg ctgcctttgg
     2701 ctagggtttt atataggctc tctctgccta gccctgtttg cttggccagc tccgccattc
     2761 ctcttgctct tgctacatcg ttcaaggctt ctaaaaattc gtcttggtcg ccctctttta
     2821 acacttccgc taaataggct tgaatagttg cctcatcggt taaaaactcc gccacgtcaa
     2881 aagggcgtaa accgtgcttt tctgctgcct gttcattgat ttgtattgtt tccattgctt
     2941 tctcctgtag tttttaattc agcccataat gctttcgctt tctctatatc ggcttgctgt
     3001 gttgatttat cgccgccgtg taacaagata taaattacat ttccttgtta tgcgtaataa
     3061 attctatatc cggctcctgt tgctatccgc atttcataaa gtccgcctgt accggctaaa
     3121 gatttatgat cgccaaagtt gccattaatc gcatttttta tccgtcttgc taccgttgct
     3181 tttgcgatag ggttctttaa attctcaagc cattttgaaa aattatcggt aataataatt
     3241 tgattagtgg tttgctctct catcattgcc ggcttcttat tctatttatt gcttattgta
     3301 tccattaaga tacagtgagc aagggttttt gccatttacc cacttttaac ccactttacc
     3361 cactgtcatt ttttctttat ttttcagtta gttataaaaa aataatagtg aaaagtgggt
     3421 aaagtgggta aaaatcgaaa agtttacaga attttcatca aggggaatta cgggcataaa
     3481 aaagccgtca ggcggtttac ctaacggcat tacaagcggt catttttggc ggaaaattta
     3541 ctaggctatt ttcaaatgat attgcggtaa gctaccttgc gaacattcct ctacaaaatc
     3601 cgcccacact tggagcagtt taaaacgttg ctctaggcgt tcgcctctat cataggcggc
     3661 tttgattcgg tcgctgttta aatgcgataa ggcaacctcg attaaatcgc tattgtagcc
     3721 tttctcatta agataagtgg aggcgatggc ccgcaagccg tgagccacta atttgccctt
     3781 gtagcccatt cgtttgatcg caccgttggc ggtttgagta ttgacgtgcg tttgtgggtt
     3841 cttcacgcta gggaataaat acgttttacc gccacttagc tttttaatct ctcgcaaaag
     3901 tgcgattgct tggcgtgaga gtgtgatttt atgctcaatg cctcgcttca tattttccgc
     3961 cggtatcgtc cacagtttgg ctttttcgtc tatatccgcc cattttgcct tagccgcttc
     4021 attcgggcgg gtcattgtga gtaactgcca cagaattaaa tagcgtgtct gtaaatgaat
     4081 ttgggcgttt tgtaaggtgt ataaaaactc gcctaaatct tccggcttaa tggttttgaa
     4141 atgttcaacc gttgggctat caaactcttt cgcaatattg gcggtagggt taaacggtat
     4201 catttcacgg tgtacggcaa acatcataat ttcattatgt ttttgaattg cccgttttaa
     4261 cttctctagt ttgccctcgt cttggtattg cttgaatgcc ttgattgcca ttggtgcggt
     4321 tatttgtgtg ataggcattc ttccaaagtg cggcaagagg ttatttttaa ttaaggcgat
     4381 tacatctttt tggtagttgt ctgaaaaatt cttcttggtc ttgcggtgtt ccagccacgc
     4441 ccacgctaca ttttcataag tgttgctgat ttcgttggcc tttgcctgtt cctgttgttg
     4501 gcggtgggtt tgagggtcga tattttgagc caataatgcc ctatattctt ctctaatttc
     4561 tctcgcttgt gctaaagaaa tatcagggta agccccaatg cttaaatcat tccgcttttt
     4621 tgtgaacggt ctttcatacc taaaccgcca caatttagaa tttgaggtaa taacaagtag
     4681 taagccgtta ccgtctctta atgggctgtc ttccggtttc gcttttctta tttgggtatc
     4741 gtttaacggt gtgatttttc tagccatagt aaacctttag gagtataaag cctaaatctt
     4801 aggagtatga ttttttgttt gggagtacaa aactaaaagc gataaagcct tatttgaaaa
     4861 ggctctaaag tggttttagg agtacgaatt tttgggggta ttccggcgta ctcctaaata
     4921 tactcctaaa atcttcggat ttgattgtat cgtataggta cgcaacggac aataaaaaag
     4981 ccttgaaaca ttgattttca aggcttttta ggtatcggtt gaaccgtata ggattataat
     5041 ttggtggagc tggggggatt tgaacccccg tccgaaattt ctctaccttc agtactacat
     5101 gtttagtcta gtctttaatt tcgcttattc acagcggaca gacacgcaat gaataaacta
     5161 gctttattta atttagtgtt tcgatcctaa agcggcggcg tccacacgat ctcgttttgg
     5221 gtgacttatc tttgccctcg tcttacgagc ggaagctgag gagataaggc tttaaagcag
     5281 gttattaagc tgctaaagcg tattgttcgt cgtttgcgac tattattttg cggtttgttt
     5341 acgaggccta ccgcacctcg acatgcacct tgggcttcgc caatcccgtc gaatccaaaa
     5401 tcagccccaa aagtagcggt attctaaagg aaagcggtcg aatttgcaaa aagttttgca
     5461 agtggcggaa cttagcggta ttgaatagct cgaaataaaa tcatacaaat cccacaccag
     5521 atagatttaa ctctgttata atgtaaacct tgctgagagt gaagagaaat agaatgaaaa
     5581 aatttaacga gaacgagatt gaagatgctg aaattataga tgccgagccg attgatgttg
     5641 atgagataga cgaagaagaa acatcggtag aagcaatgca tccggctttg gtttccggct
     5701 caatgcctgt gccgcagggc aatttggata gctacatccg aatggcaaat cagtatccga
     5761 ttctcaccgc cgagcaagaa aaagaactgg cggagcgtta ttattatgat gaagacgttg
     5821 aggcggcaaa acagctgatc ctttcccata tgcggtttgt gatccatatt gctcgtggct
     5881 atttgggcta tggtttaccg ctggcagatc tgatccaaga gggtaatatc ggcttgatga
     5941 aagcggtaaa acggtttgac ccgaatgtcg gcgtgcggtt ggtttctttt gcggttcatt
     6001 gggtgaaagc ggaaattcac gaatatgtgc tgaaaaactg gcgaattgtg aaagtggcaa
     6061 ccaccaaagc tcaacgtaaa ttattcttta atttacgcaa aaataaaaac cgcttggcgt
     6121 ggtttaatga agaagagatc aaaaaagttg ccgatgattt aggggtttca gtggaagaag
     6181 tacgagaaat ggaatctcga atgaccggac aagatttagg ttttgacctg cctgccggtg
     6241 atgacgatga agacagctac gctccttcta tgtatattga agatagcagc tccaatttcg
     6301 ccgatgactt ggaagacgaa caacataatg ggcaggcaac cgctcaactt gcctatgcgt
     6361 tagccaccct tgatgaacgt agccaagaca tcatcaaaac ccgctggtta gatgacaata
     6421 aagccacact gcaagattta gccgataaat ataacatctc ggcagagcgg gtaagacagc
     6481 ttgaaaatgc cgcattgaag aaaatcaagg aagcgatttc attagacggc tagaaatgcc
     6541 tacaagcggt gattttttta ctcgctacgc tcgcacttcg tgccgtcggc agagccaacg
     6601 ttcaaacgca agcgtttgtg caaaatttta cgcaaaaagc accgcttgta ttttgactaa
     6661 aaatgaggat actatgatta acattaccga taaacaaatt gacccaactt tctaccagcg
     6721 tctaccagcg agcagacggc tttattaacg ttgccaacgc tcatttaaaa aacattgccc
     6781 caaaccaagt cagcaatgcg atgttgtttg gttgtgccag atttaacgcc tatgttgcgg
     6841 caagcaaggc ggaatataaa cagcagttgg ccgattccag agaagaagtt attcaatatt
     6901 ttgtggagca atacaaagaa atgctcactg caaatttaga tgaatatatc caaaacttcg
     6961 agcgatatat tgaagggaaa aaggcggatt aaccgccttt gttttgtggt caaacttgtt
     7021 ttacaaaatc acttaaaact tgattaattc tactttgcca acctttacct gaagctctaa
     7081 aggcttcaag aacgctttta tcaagacgta acgaaaccaa ttctttcttt gaaccagctg
     7141 gtctgccacg ttttttcatt gcaattgaaa actctgatgg cgtaaccggg cgttcatcgt
     7201 catcttttcc gtcccaaata aaatcttggc ttgccggaat agcttgaata gccataagta
     7261 gctgtttttt ctctaaattt ttattattca aagtattgtt gtaagtattc ataatatgcc
     7321 tctctttctg ctttagatgc tcgccgaaag ctaattactc tgatgtaatc ttccctttct
     7381 gtatatgcaa gtgataatac gagtaaacgt ccaaatgaat atgcaaaaga ttgtattcta
     7441 aattcattgt tacgaataac ttcaacatca aatcgatagg gcgatgagag aacttcatct
     7501 gcatcggcaa aatctaatcc gtgcttggct aaattgattt tgcgtttagt ttcgtcccaa
     7561 gtataaattt tcataattat tgtatataca taaaataaat ctaacaagaa ctaaatttaa
     7621 tttagcaaac agtaatttga aaatcagatt agaaatacat tttttcgatg cagatcgcaa
     7681 aaataaaatg aaatttaact gcttgaagag tctactgagc cattttcccc gctaccgtta
     7741 aaaaaccatc tgcggtttta aaaatttcac cgaatccttg agcctccaaa atttggtcgg
     7801 agacttcacg aaacgcccct tcaccaccgg ctttttgcaa tacccaatct gctttttctt
     7861 tgatgtaatc gagggcgtta cgggttgcaa tcgcaatgcc acacacttca aaagccggta
     7921 aatctaaggt gtcatcacca atgtaagccg tttgctcagg ggtaacaccg gcttgctgca
     7981 ttagctcaaa gcaggcggag cgtttttcca ttttgcctaa caaagcgaga tcaattttca
     8041 gcacctctaa ccgtttgcgg agcaaagccg tatcgccgcc cgagagtact gccactttaa
     8101 tgccacaact ttgtaacatc ttaacgccta aaccatcgtg aacgtggaaa cgtttcatta
     8161 cttcgccttg gtcggtgtag tacattccgc catcggttag cacgccgtcc acatcggtaa
     8221 tcactaattt aatcttggtg agatctaaca tttttttgcc tcaaatatgg tatgatttta
     8281 ccattatagc ttttttacct ccgtttacca aaggaaccga aatgtcgatt gccgagaatt
     8341 tatcgcaaat ccatcaacaa attcagcaaa tttccgaaca atatcaacga gataacgtgc
     8401 gtttgttagc cgtctccaaa actaaaccag ttgaggcgat tcaagcggcg attgaggcag
     8461 ggcagcgtgc atttggcgaa aattatgtgc aggaagcggt cgaaaaaatc gaattttttg
     8521 caaatcgccc cgaattagaa tggcatttta ttgggccact tcaatcaaat aaaaccaaat
     8581 tggtcgctgc ctattttgat tggattcaaa cggtggatag attaaaaatt gccgagcgtt
     8641 taaacgaaca acgcccagcg gataaagccc cgctcaacgt gctgattcaa attaatatca
     8701 gcgatgaagc ctcgaaatcg ggcattgaac cagatgaaat gctaccactt gcccaagcga
     8761 tttctcaatt accgaatttg aaactgcggg gcttaatggc cattcccaaa ccagaaagcg
     8821 agccagagca acaaaaagtt gcctttcgca aaatgcagct actttttaac cgcttgcaaa
     8881 cagaatttga cgacattgat accctttcaa tggggatgtc ggacgatatg caagcggcga
     8941 ttgaatgtgg ctcgacaatg gtgcggattg ggactgcgat ttttggggcg agacgttagc
     9001 cagctgagtt tttacataaa gctgatacaa gcggtcaaat tcgccaaaaa ctttgcaaat
     9061 tccaccgctt gttttgtagc caacagcgga aaaagtctgt ataattacgc ctcctaattt
     9121 cttgactctc gccagttttg atgggcgaga gtttttattt tgacttaact taaagatatt
     9181 ccaatgcaaa atgtagatat ttcaaaattg cgtaacattg caattattgc ccacgttgac
     9241 cacggtaaaa ccaccttggt tgataaactt ttacaacaat caggcacctt tggtgaaact
     9301 cgtggtgacg ttgatgagcg tgtaatggat tccaacgatc ttgaaaaaga gcgtggcatt
     9361 accattcttg caaaaaatac ggcaattaac tggaatggtt atcacatcaa tatcgtagat
     9421 accccagggc acgctgactt cggtggtgag gttgagcgtg tactttcaat ggtagattct
     9481 gttctgttag tggttgatgc ctttgatggc ccgatgccgc aaacccgttt cgtcacgcaa
     9541 aaagcgttcg ctcacggttt aaaaccaatc gtagtgatca ataaagttga ccgcccaggt
     9601 gctcgccctg attgggtggt ggatcaagtg ttcgacttgt ttgtaaattt aggtgcaagt
     9661 gatgagcagt tagatttccc aattatctac gcttcagcct taaatggtgt agcaggctta
     9721 gaacacgaag acctagcggc agatatgacc ccactttatg aagcgattgt caaacacgtt
     9781 caaccaccac aagtggaatt agatgcacca ttccaaatgc agatttcgca attagactac
     9841 aacaactatg tgggcgttat cggtattgga cgtatcaaac gtggttcggt gaaacctaac
     9901 caagcggtaa cgattgtaga tagcgaaggc aaaactcgcc aaggtcgtat cggtcaggtg
     9961 ttggggcatt taggtttaca acgttttgaa gcaacagaag cctttgctgg cgatattgtt
    10021 gcgattactg gtttaggcga attaaatatt tcagatacta tttgtgatat taacgcagta
    10081 gaggctttac cagcattaag cgttgatgaa ccaacggtaa cgatgttctt ctgcgtaaac
    10141 acctcgccat tctgcggtca agaaggtaaa tttgtgactt cacgccaaat tcttgagcgt
    10201 ttaaacaaag aattagtcca caacgtggca ttgcgtgtgg aagaaacttc gaacccagac
    10261 gaattccgtg tgtcaggtcg tggcgaatta cacttatcgg tattaatcga aaatatgcgt
    10321 cgtgaaggtt acgagttagc agtgtctcgc ccgaaagtaa tctaccgcga agaagacggc
    10381 aaaaaacaag agccgtttga gcaagtgacg attgatatcg aagagcaaca ccaaggttca
    10441 gtgatggaag cacttggtat ccgtaaaggt gaagtgcgtg atatgattcc ggacggcaaa
    10501 ggtcgtaccc gtttagagta tgtgatccca agccgtggct taatcggttt ccgtaacgaa
    10561 tttatgacga tgacctcagg tactggttta ctttactcta cattcagcca ctatgatgac
    10621 gtaaaaccgg gcgaaatcgg tcagcgtaaa aacggtgtgt taatttctaa cgcaacgggt
    10681 aaggcattag catacgcatt atttggctta caagagcgtg gaaaattaat gatcgaccac
    10741 ggtgttgatg tatatgaagg gcaaattatc gggattcaca gccgttcaaa cgatttaacc
    10801 gtaaactgct tacagggtaa gaaattaacc aatatgcgtg catcaggtaa agacgatgcc
    10861 atcgttttaa ccacaccggt gcgtttcact ctagaacaag cgttagagtt tattgacgat
    10921 gacgaattag tggaagtaac accacaatcg gtgcgtattc gtaaacgttt attaactgaa
    10981 aacgaccgga aacgtgcaag ccgtactaca accagtacca gcacgcacta actgataaaa
    11041 aagagcgact gaggtcgctc ttttggtttt aaattctccc tacaaatata acgccattac
    11101 caccgcattt tctttgctac cgtttggcgt ggggtaatag tttttgcggg tggtaacttc
    11161 attaaaaccg agtgaatcgt agagttttct ggcagaatgg ttggattctc gcacttccaa
    11221 ccacagggtg gtgagtttcc gttgttgcag ttgcaaaatt aattcggaaa gtaaccgctt
    11281 gccaatgcct ttgccttgaa aatcaggagc aactgcaatg ttaaataagg ttgcttcatc
    11341 tagcacgata tgactaatgg caaaagccac aattttgttt tgctctacca cttttaaatt
    11401 caggtatttt tcaccctgat tatttaataa cgtccccttt gaccacggca ctaaatgggc
    11461 ttgttgctca atttcaaata actgctcgaa atcttccgct tgaatttgct caatcacgct
    11521 gttctccaaa ttgtgccgaa aaagtatcaa tttgttgcca aagttggcgt ttggtcgtag
    11581 atttggctaa atcttgccaa gactcgtttt tccaagcggt ctgattagta aaactttttg
    11641 ccattttgac cgcttgttct tccgcttgaa tcagccaaaa gatcggcgaa tgctcaaaca
    11701 ccaaacgttg tgattgttcg ccagtcagcc attgatattc gctgtttttt aactttaaag
    11761 cacgcagaat atcggcaaag agccggctgt gttggtgatc ttcctcacac actaccacca
    11821 attttaccgc attgtttagg cggatttgag catcgccttt tagcacttgg ggattggtta
    11881 gcacccattg cgaaataccc atttcctgta acagtaaatc acgacgattc atttaaaacc
    11941 tcatcaaatt ttgcaaatag tatcaaaact ccgcttgaac agaaactttt ttcaaaaaat
    12001 tcaagatttc tgtacttaaa ttcggtatat tcacgctaaa ttttttagat aaggtaagaa
    12061 aatgctctct ttagaaagcg aagtacttga acgccatttg gcgttatttg aaaacaagtc
    12121 ggtgttatta tttggcgatg tgcgagatga ttttgcccgc cagctgcaat ctcgtgcgga
    12181 aaaggttgcg gtattcagta gctattttga ttacgcacgc caccatagtg aggttgaatt
    12241 tggcttagaa tgtggtttga gtgccgagtt agcggtgttt tattggacga agaacaagca
    12301 ggaatgccaa ttccaactgc tacaatggct gtcgcaatgc caagcagggc aagaaatgtt
    12361 gattatcggc gaaaaccgag ctggtgttcg ttcggtagaa aaactgctcg aaccgttcgg
    12421 caatattgcc aaaatcgact ctgcacgccg ttgtggttta taccattttg agctacagac
    12481 cgtgccgaca ttcgattgca aaaaattctg gaaatcctac cgcttgcaag acttagatgt
    12541 gttcgatcta ccagcggttt ttagctcggc ggagctggat aacggcacca aattattgct
    12601 ttcaaccttc gacaaagcag acaggctaaa aggcaaagtg ttagatttag gctgtggggc
    12661 tggcgtgatt ggggcaacgc tcaaacaaca atttccgaaa atcaaactga cgatgagcga
    12721 tattcacgca atggcgttgg aatccggcag acgtactttg gcggaaaatc agttagaggg
    12781 ggaagtggta gcaagtgatg tgttctctca cattaatgaa cggtttgatt taattgtctc
    12841 caacccacca ttccacgacg gggtcgatac cgcctaccga gcggtggagg agttgatttt
    12901 ccaagctaaa aatcacctaa ccaaaggcgg tgagctgcga attgtcgcaa acgcattctt
    12961 gccttaccca gatttgctcg ataaagcctt cggcaaacac gacgtactgg caaaatccac
    13021 caagtttaag gtgtattccg caagaacata ataggcagaa caaacggtta taaaaaagcc
    13081 ggtatttgct taaaaaagtg cttgatattt ctgccggctt gacgtaaatt ctgttcatac
    13141 tgttttggag taatgcaatg aatttaaact acactttaca tcaccaccac actttaacct
    13201 gatatctttc gggcatttgg tgtgtgcgga agataactct tccgagcaag tgtagcaaat
    13261 c
//
LOCUS       DANLUA010000058        13180 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLUA010000058 DANLUA010000000
VERSION     DANLUA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..854)
                     /locus_tag="PXB44_001179"
     CDS             complement(<1..854)
                     /locus_tag="PXB44_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDL5281664.1"
                     /translation="MMSFLSEVFSSFKPTKTEVISSEPNENSGESDIIQNKMSEVNNE
                     VQQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDVNPYVVQFYCQDSDDLQPYIEQLRNYIS
                     PIAKNSAFSEEWLRVQAKHFKDIGKPEGLFIDERVTNTAWSGRIRSTKMVIYRYVDKS
                     NDTNENLIVEQLNNACSRINGALTTAGLQLQRLDGEQFRQWLLRWFNPNPSINFEGLS
                     NNDCYHLFG"
     gene            complement(854..1252)
                     /locus_tag="PXB44_001180"
     CDS             complement(854..1252)
                     /locus_tag="PXB44_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5281665.1"
                     /translation="MKALYCIMMSALLITGCTTSTEELLPANGSTMRDIWDKGAGNND
                     LHLYRSQQGRTLDPVDYIPNTEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(1265..1837)
                     /locus_tag="PXB44_001181"
                     /pseudo
     CDS             complement(1265..1837)
                     /locus_tag="PXB44_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
     gene            complement(1815..2321)
                     /locus_tag="PXB44_001182"
     CDS             complement(1815..2321)
                     /locus_tag="PXB44_001182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281666.1"
                     /translation="MAYQTLKSTQSQTQEKSDERAKQLLGSRVVEQVSESERENGNGR
                     EISSGSRQTSNEQRTGYSGNSSQEYRQISKSGERLTNKTSENALIEPTRPIKNKNGSK
                     VAPTPTNTPKTQNNANSNLLSSEQYRQTMEFLREFNDRLEQIAQNILENQLKALRTKA
                     KPSHCQWY"
     gene            2341..2490
                     /locus_tag="PXB44_001183"
                     /pseudo
     CDS             2341..2490
                     /locus_tag="PXB44_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            2491..3084
                     /locus_tag="PXB44_001184"
                     /pseudo
     CDS             2491..3084
                     /locus_tag="PXB44_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
     gene            complement(3238..4053)
                     /locus_tag="PXB44_001185"
     CDS             complement(3238..4053)
                     /locus_tag="PXB44_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDL5281667.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(4389..5225)
                     /locus_tag="PXB44_001186"
     CDS             complement(4389..5225)
                     /locus_tag="PXB44_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDL5281668.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5225..6028)
                     /locus_tag="PXB44_001187"
                     /note="aph(3'')-Ib"
     CDS             complement(5225..6028)
                     /locus_tag="PXB44_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDL5281669.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(6197..7042)
                     /gene="sul2"
                     /locus_tag="PXB44_001188"
     CDS             complement(6197..7042)
                     /gene="sul2"
                     /locus_tag="PXB44_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDL5281670.1"
                     /translation="MEYTSKSQIARNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQA
                     RKLMAEGADVIDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQ
                     AYALSRGVAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHI
                     AAFFDARIAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLL
                     SVSRKSFLRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKE
                     TARIR"
     gene            7205..8497
                     /locus_tag="PXB44_001189"
     CDS             7205..8497
                     /locus_tag="PXB44_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL5281671.1"
                     /translation="MGTYIPHREGIIQTIVRNSLTQYQAYASPSQTILDRLEHFAECA
                     DWTKGVVRIRCHDCGHSYFRPFSCKVFHLCPSCDQKRTLLYAEYLAEDLLLDLPHRQF
                     VFTIPKILRPYFKSDKRLFGEVSRLVFSLLSDFFSLAAGQELLCACVVSYQSFGEFAR
                     FHPHWHVLVLEGGFTKYDRFVYLPIGADEGMLKVWQAAIMALFLRKELIDQARVNMLR
                     DWKHSGFSIESETRLFNKADREALGQYVVRGATCAEKIQYDPASDTIIWTASPKGFYK
                     GKTETFKGFEFVDQLVAHLPPRRVQLLRRYGVYAGKVRKQWQERPNIYSLAPESWQKG
                     HPRESKIVPAIPQEKPETVQVSDAWSKLRKQSWARLLQKVYEVDPFVCPKCQGTMSVV
                     AIIEDPKELAKIINWAKQQEREQPVAVCARSPPELALV"
     gene            8837..9094
                     /locus_tag="PXB44_001190"
                     /pseudo
     CDS             8837..9094
                     /locus_tag="PXB44_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            9563..10009
                     /locus_tag="PXB44_001191"
                     /pseudo
     CDS             9563..10009
                     /locus_tag="PXB44_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            10286..11500
                     /gene="floR"
                     /locus_tag="PXB44_001192"
     CDS             10286..11500
                     /gene="floR"
                     /locus_tag="PXB44_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL5281672.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            11528..11833
                     /locus_tag="PXB44_001193"
     CDS             11528..11833
                     /locus_tag="PXB44_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5281673.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            11945..>13180
                     /locus_tag="PXB44_001194"
     CDS             11945..>13180
                     /locus_tag="PXB44_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL5281674.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRV"
BASE COUNT         3053 a         3480 c         3487 g         3160 t
ORIGIN      
        1 ccaaaaagat ggtagcaatc attgttgctt aacccctcaa aattaataga aggattggga
       61 ttaaaccaac gtaacagcca ttgacgaaat tgttcgccat ccagtcgctg caattgtaat
      121 ccagcagttg ttagagctcc atttatgcga gaacaagcat tgttcagctg ctcgacaatc
      181 agattttcat tagtgtcatt ggatttatca acataccgat agatcaccat tttggtacta
      241 cgtatacgac cagaccaagc tgtatttgtc actcgctcat caataaaaag cccctctggt
      301 ttaccgatat ccttgaaatg cttggcttgt acccgtaacc attcttcgct aaatgcacta
      361 tttttagcga taggcgagat ataatttcgt agctgttcaa tataaggctg taaatcatcg
      421 ctgtcttggc aataaaattg aacaacgtac ggatttacat ccaattcatc aaagctgtct
      481 tgcaaggcat ctttgacaag attacgtatc cgacttaaaa attcaatact acggccagca
      541 gtaccaatcg gcttaatatc aaatactgcc ccaacactac gggcatcatc aaggagtatc
      601 acaccgtgct cattcaaata ttctccccaa ggtagatgat cgataaagga gggctcttgt
      661 gagtataacc gacggtgatg ctgtgactgg gtgagttttc cactgcgttt taattgttga
      721 acctcgttat ttacctcact cattttattt tgaatgatgt cagactcacc tgaattttca
      781 tttggttcac ttgatataac ctctgtctta gtcggtttga aagaggaaaa tacctcactt
      841 aaaaaagaca tcatcaaagc cctcttgttc gatcacccgg ttgagcatat tggactcgcc
      901 cgtagaaagg taataccgta gaatatcctg gaatactaag aggctctccg gaacgagttt
      961 ggtgtggata cacatacata atcagatccg gattaggtaa acgagggaac aagttattaa
     1021 tttcattttc tgccgtacgg gtataggctc gttgctcagt gttaggaata taatcgacag
     1081 gatcgagtgt tcgtccttgt tgtgaacggt ataagtgtag atcgttatta cctgctcctt
     1141 tatcccaaat atctcgcatc gtactgccgt tagcgggtaa taattcttca gttgaagttg
     1201 tgcaacctgt tatcaataag gcactcatca ttatgcaata taaagctttc attgtttgtt
     1261 ccttttaatc aagttgtgta ttagttgatg tgtttccgta tttcacttta cgtcctttgg
     1321 agttgtaatc gatctcaatc tctttctcga tatgaatagc gacacgatgt ccaggtggta
     1381 catagacagc atcaaagttt tcagcaaaac gctttctaaa ccagtcggcg gattctttta
     1441 aaccaccgcc gatggcttgc cctaagatga acttaccttg atcaccggta acagctccaa
     1501 caacactatt gccatcaact actgtcgttg tttgtgattg tgaaaaccct tgggctgcgg
     1561 cactggcagc cgctaagaag aaatttgtgc ctaaatattc tggtgcattt gtttttctct
     1621 ccccgggaac acaaggaatg cctgaactat cagataacca accgatattc tcactattcc
     1681 ctttgttagt cgtgctgaca atacgcccat cattgaacac aaaggtcata ctttttacat
     1741 cgcctcgtac acaagataga gtccagtcgc cagtagctgt acccgaaaca atcgcacctt
     1801 ctacatcaag taattcaata ccattggcaa tgagatggtt tagccttagt tctcaatgct
     1861 tttagctgat tttctaggat attttgggcg atttgctcca atcggtcgtt aaattctcgc
     1921 aaaaactcca tagtctgccg atactgttct gatgacaata gattagaatt tgcgttattt
     1981 tgcgttttag gcgtgtttgt tggtgtgggt gctaccttac taccattttt atttttgata
     2041 ggtcttgtag gctctatgag agcgttttca gacgttttat tagttaatct ttcgcctgat
     2101 tttgatattt ggcgatattc ttggctagaa ttgcctgaat atcctgtccg ctgttcgttg
     2161 ctcgtttggc gacttcctga cgaaatttct cgtccgttgc cattctctcg ctcgctttcg
     2221 ctgacttgtt caaccactct tgaacccaag agttgttttg ctcgttcatc tgatttttcc
     2281 tgtgtctgtg attgtgtgga ttttaaggtt tggtaagcca aaacttgctc gttatacgcc
     2341 aaggaggaca cccggcgcaa ggtgctggta ggcgcgtgga tactcggcaa ggttgaaagc
     2401 ggcgagtggc cagaacagcg cctgctcgat gggttggata gctacctaga gcgcgaccat
     2461 gatcgggcgc tgttcggact gccacccaag gggtcgattt gcggccacac ctttgcggta
     2521 aatgaaaaag gtatcgttaa cacggtgaat aacatccgtg cagtgtatcg tccgcaggga
     2581 ttaccgcgtc agatcctggc tcgcgcctcg ctgaatgcga acaccctcga cgaggccatc
     2641 actatactta ccggccagcc gcgatccggg gcgtttcacc atacgctcgg gcagctgggc
     2701 gatgcgcggc tgttcagcgt tgaggccacc ggtcaaggct gttccgttct acccctgacg
     2761 acggtaaccg ggcatgccaa ccatctggtg catgcggcac tggctggcgt tgagcagatc
     2821 gttaccgaca gttcagcgtc gcggcagtta cgcctcgtgc agtggcggga aacgcaacct
     2881 ccgtttgacg ccgcggcggc aaaaaccatt ctctcagata cccatgacgc agaactgcca
     2941 atttaccggc tggctgccga cgatccggat gaggagaaca ccctcgccac ggcagttttc
     3001 accctcgatg ccaaccacgt caggtggcaa attttcgaca ttaaccgcga cgatgctaaa
     3061 tttcatggag aagtgcgtgg gtgaaatagc cggtcgtcag tcataaaggg caggggagta
     3121 ccgttggccc ggcgttcgat agtaccgtcc ggatactgcc agatatcggc gtccagggag
     3181 atgtcctgaa gcgcggtgtc cggaatttca ggtttgtgtc tctacaaaga ctaactatca
     3241 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     3301 atatttttga aaaagccgtt tctgtaatga aggagaaaat tcaccgaggc agttccatag
     3361 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     3421 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     3481 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc
     3541 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     3601 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttactaacag gaatcgaatg
     3661 caaccggcgc aggaacgctg ccagcgcatc aacaatattt tcacctgaat caggatattc
     3721 ttctaatacc tggaatgctg ttttcccggg gagcgcagtg gtgagtaacc atgcatcatc
     3781 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aatgccgtca gccagtttag
     3841 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     3901 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     3961 atcgcgagcc catttatacc catataaatc agcatccagg ttggaattta atcgcggcct
     4021 cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     4081 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatat aacataagag
     4141 attttgagac acaacgtgga tttgttgaat aaatcgaact tttgctgagt tgaaggatca
     4201 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
     4261 caactggccc acctacaata aaggggtcgt ttgcgggaga gggcgaaatc ctacgctaag
     4321 gctttggcca acgatattct ccggtaagat tgatgtgttc ccaggggata ggataagtcg
     4381 cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg
     4441 cgttgctcct cttctccatc cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc
     4501 tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt
     4561 gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag ccggatcgta gaacatattg
     4621 gcggcgccaa agcccacttc accgaccaga ccgacgggat ctatcaccag ccagccgcga
     4681 ctggagaaca tgatgttttc atgatgcaga tcgccatgta gcccacgcag ttccgaggca
     4741 ttgctcatca tttgatcggc tataatcgcc gcgtggacgt agtcagtttg acaacctgcg
     4801 ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg
     4861 gcagaaggca ggggttcctc agatgcggca tacagcttcg ccatcagttc cgctgcaatt
     4921 tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag agagcattcg ctccccggca
     4981 tattcgagca acatcagatt gttctcacga ccgagcaacc ggactgctcc cctcccattg
     5041 cgccatacca gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat aggtttcaat
     5101 cccttgacga ttgcaggagt cccgtctggc aatgaaactt tccaaacgag gctggaaaag
     5161 gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg
     5221 aacatcaacc ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg
     5281 gggcttcgat ccccaataca ttgaatagga cagcgaaggc gcgctctgct tcatctggcg
     5341 ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa atcggcatag cgatctgctg
     5401 ttccgagccg cccaaggtcg atcagacccg tgcattgaag agttttaggg tccaccatga
     5461 agttcggcat gcagggatca ccatggcaaa caaccatatc ggtgcgctct tggtcgagcc
     5521 gcaccggtag ctctcgttcg acacgagcca aaagatcgag ctgcggcgta ctcttgtcct
     5581 cgtccggtaa gaagtcggga ttgacggcat tgcgggacac cacatcaacg gcgcgtccga
     5641 acattcgcga cagcctgcgc tcaaacggac attgatcaac cgataggctg tgaacagcgc
     5701 caagttgctg ccccattgac ggccacgctt tgagcaaatc cgctccagac agatcagccg
     5761 ccggtactcc cggaattgcc gttatcacca agcatgcacc ctcctgttcc tcctgccagt
     5821 tgatcacctc ggggcaagcc acacctcgac ctttgagcca aatgaggcgg tcacgctctc
     5881 cagcgagctc accgcggcgg gaagcaggtg cgattttcgc gaaggcatgc ccgtcaccac
     5941 gtcgaaaaac aaaatcaccg gattctccgc ctctgacagg caaccagtca gaatgcgatt
     6001 caccaaaaaa aatattagtt cgattcaatg gaggttcctt cagttttctg atgaagcgcg
     6061 gaggtggctt tgccacgaat accatctgtg ccgaaatatt tgcgcaccat aaggtcgatt
     6121 atcctgtcgt cgggtcgccc tcaaagggga catgcctgct gaaccgcgaa tatagagaaa
     6181 tatcccgaat gtgcagttaa cgaattcttg cggtttcttt cagcgccgcc aataccgcca
     6241 gcccgtcgcg caaggggcgc ggctcgtgtg tgcggatgaa gtcagctcca cctgcggcgg
     6301 cggcaagctc tgcagcgagt gtcgcggccc cgacatcccc cggaccacgg cctgtgagcg
     6361 cgcgcagaaa ggatttgcgc gaaacagaca gaagcaccgg caaatcgaag cgcagccgca
     6421 attcatcgaa ccgcgccagc accgagagcg aggtttcggg agcagccccc agaaaaaacc
     6481 ccatgccggg atcaaggaca aggcggttgc gtttgatacc ggcacccgtc agcgccgcga
     6541 tgcgcgcgtc aaagaacgcc gcaatgtgat ccatgatgtc gccagcgggt gcctcgcgcc
     6601 gatctgcctg cccgtcttgc accgaatgca taacgacgag tttggcagat gatttcgcca
     6661 attgcggata gaacgcagcg tctggaaaac cgcggatatc attgagatag gccacaccac
     6721 gcgacaaggc ataggcttgc gtcgcgggtt gataactgtc gagcgagacg ggaatgccat
     6781 ctgccttgag cgcgtccagc accggcgcga tacgcgcgat ttctgtgtcg gacgaaacag
     6841 gcgcggcgtc gggattgctg gatgccggac cgaggtcgat cacatctgcc ccctcggcca
     6901 tcagcttacg cgcctgcgca atggctgcgt ctggcgccag ataccggcct ccatcggaga
     6961 aactgtccga ggttatgttg acgatgccga aaatgatgag cgatttattt cgagctattt
     7021 gtgatttact tgtatattcc attttcctcc gcgccgaagg cgtcaaccta actttcggtt
     7081 gagctgcgaa cgggcttgtc ccgtgagtcc gctcgaaccg gttgttaggt ggcgccagtc
     7141 cacattttcc attattttct tcgcaattgc aagcaaatga tcgttttcct gcgtatgtat
     7201 aggtatggga acttacattc cccatcggga agggataatc cagaccatag tccgcaattc
     7261 tctaacccag taccaagcct acgcaagtcc atcgcaaact atccttgacc gccttgagca
     7321 ttttgctgaa tgtgccgatt ggaccaaggg tgttgttcga atacgttgcc atgattgtgg
     7381 acattcgtac ttcaggccat tttcgtgcaa ggttttccac ctttgcccat cctgtgacca
     7441 aaaacggacc ttactctatg ctgaatacct ggccgaggat ttgctccttg atctaccgca
     7501 tcggcaattt gtttttacga ttcccaagat tctcagacca tactttaaat cggataagcg
     7561 actgtttggc gaggtgtcca ggctggtatt ctccttgctt tctgattttt tctccctggc
     7621 agccggtcaa gaactactat gtgcctgtgt tgtcagctat cagtcgtttg gtgaattcgc
     7681 tcgattccac ccgcactggc atgttctggt tctggaaggt gggttcacca agtacgaccg
     7741 ctttgtatat ttaccaattg gagccgacga agggatgctc aaggtctggc aagcagcgat
     7801 tatggctttg tttctgcgaa aggaactaat agatcaagcc cgagtgaaca tgctcagaga
     7861 ctggaagcat tccggcttta gcatcgagag cgaaaccaga ctattcaaca aggccgatcg
     7921 agaagccctc ggccagtatg tcgttcgcgg cgctacctgt gcagaaaaaa ttcagtatga
     7981 tccagcttcc gacacgataa tctggacagc tagcccaaag ggattctaca aaggaaaaac
     8041 agagaccttc aaaggctttg aattcgtcga ccagcttgtg gcccatttac caccaaggcg
     8101 ggttcaattg cttcgtcgct atggcgtata tgcgggcaaa gtccgcaagc agtggcaaga
     8161 gcgccccaat atttatagcc tggccccaga aagttggcaa aaaggtcatc cacgcgaatc
     8221 caaaattgtt ccagccatac ctcaggagaa accggaaact gtccaagttt ccgatgcctg
     8281 gtccaagttg cgcaaacaaa gttgggcacg gttgttacag aaagtctatg aagttgaccc
     8341 gtttgtttgc ccaaaatgcc agggaacaat gtcggttgtg gcgataatcg aggatcccaa
     8401 ggagcttgcc aagattatta actgggcaaa gcagcaggaa cgggagcagc cagtggccgt
     8461 ctgtgctcgt tcacctcctg agcttgcttt ggtgtaggta taatacccaa aaacgaagtt
     8521 tatgcggaac aggataacac ctcgaaatcg gagtttatat ggcttcgtgg gggagttatg
     8581 tgtttgagct ttgtcgcatt aacgcgaatc aggggttgac ggcagaaata gactggaatt
     8641 acacttcttg gagtcggcgt tgccggaaaa ttctgattgg attagttgtt cggggtgcga
     8701 aaacagtcgt agttcgggga aaaaaccgaa ttttgacccg aaaccgcaaa aaacccgaaa
     8761 tgacagttcc tataatttat ttatttgggg gcgtgcacct gtagccttag cgactttaaa
     8821 tgacagttcc tataattctg atgaaccggc aacaccagca caacggcgag cgtcgatgac
     8881 atgggcgcaa cggctcaagc gtgttttcaa tatcgacatc gagacctgca gcggctgcgg
     8941 cggcgccatg aaagtcatcg cctgcattga agaccctata gtgatcaagc agatccttga
     9001 tcacctgaag cacaaagccg aaaccagcgg gaccagggcg ttacccgaaa gccgggcgcc
     9061 accggctgag ctgctcctgg gtctgtttga ctgacgagcc tgaaggccaa cgataccaat
     9121 caaaatgctg cgttcacagc gccgcggcag ggatccgccg tgctggttgt cggaaaagga
     9181 gccgctagtg ggaaagagga gggtaaattt tcagcgttgc tggctccccg tcagccggat
     9241 tgggttgcat cgcaggggtg tcgaaagagt caactgcggt ccaaagctgt tggacttggg
     9301 tgaaaagggc gtttattctt cctatacgtg ccgcgttcca ggcggcgact atgagggcta
     9361 tgtcgatgcc catgtgcgcc ggctggaggc gctacgccgg gccggtatcg tcgagcggat
     9421 cgacgccgac caatggcgca tccccgatga tctggtcagc cgtgccgccg cccatgacgc
     9481 cggccgagac agtcaggcca gcgttcgcgt cctttccccg gtcgatctga acaaacagat
     9541 cggatcggac ggcgcgacct ggctggaccg gcggctgatc cacggcgaga cggccgacct
     9601 tgcgccaacc ggcttcgggc aacaagtccg cgaagccatg gaccagcgcc gcgagcacca
     9661 tatcgaacag ggcgacgcca cccgcagccg ggacagccgc gtcttctacc ggcgcaacct
     9721 tctcgccatc ctgcgggagc gcgaggtagc cggcgtcgga tcggatatgg ctttgagtaa
     9781 gggcctgccg ttccgcgccg ccacggacgg cgagagcgtc agcggcaagt ttaccggaac
     9841 cgtgcatcta tcgagcggca agttcgccgt ggtcgagaaa tcccatgagt tcacccttgt
     9901 cccgtggcgg ccgatcatcg accgccaact cggccgcgag gttatgggca tcgtgcaggg
     9961 cgggtcggtg tcgtggcagt tagggcggca gagggggctg gaacgctgag tgcgcccatg
    10021 ccgcattgcg aagcaaaaga taatcggata aaatgtagca attcatattc gtaagcgtgg
    10081 agtaatcaga tgggaaattc caagtcagca gacaagtaag ccgcaacaac cagtattgtt
    10141 gttgcggcgc tctgtaaggc tagtctcatc tgattgctga cgagcagacg tcgcccggta
    10201 ttccttaatc gagggttgat tcgtcatgac caccacacgc cccgcgtggg cctaaacaaa
    10261 tatcaatcgc aaggagatca tattcatgac caccacacgc cccgcgtggg cctatacgct
    10321 gccggcagca ctgctgctga tggctccttt cgacatcctc gcttcactgg cgatggatat
    10381 ttatctccct gtcgttccag cgatgcccgg catcctgaac acgacgcccg ctatgatcca
    10441 actcacgttg agcctctata tggtgatgct cggcgtgggc caggtgattt ttggtccgct
    10501 ctcagacaga atcgggcgac ggccaattct acttgcgggc gcaacggctt tcgtcattgc
    10561 gtctctggga gcagcttggt cttcaactgc accggccttt gtcgctttcc gtctacttca
    10621 agcagtgggc gcgtcggcca tgctggtggc gacgttcgcg acggttcgcg acgtttatgc
    10681 caaccgtcct gagggtgtcg tcatctacgg ccttttcagt tcggtgctgg cgttcgtgcc
    10741 tgcgctcggc cctatcgccg gagcattgat cggcgagttc ttgggatggc aggcgatatt
    10801 cattactttg gctatactgg cgatgctcgc actcctaaat gcgggtttca ggtggcacga
    10861 aacccgccct ctggatcaag tcaagacgcg ccgatctgtc ttgccgatct tcgcgagtcc
    10921 ggctttttgg gtttacactg tcggctttag cgccggtatg ggcaccttct tcgtcttctt
    10981 ctcgacggct ccccgtgtgc tcataggcca agcggaatat tccgagatcg gattcagctt
    11041 tgccttcgcc actgtcgcgc ttgtaatgat cgtgacaacc cgtttcgcga agtcctttgt
    11101 cgccagatgg ggcatcgcag gatgcgtggc gcgtgggatg gcgttgcttg tttgcggagc
    11161 ggtcctgttg gggatcggcg aactttacgg ctcgccgtca ttcctcacct tcatcctacc
    11221 gatgtgggtt gtcgcggtcg gtattgtctt cacggtgtcc gttaccgcga acggcgcttt
    11281 ggcagagttc gacgacatcg cgggatcagc ggtcgcgttc tacttctgcg ttcaaagcct
    11341 gatagtcagc attgtcggga cattggcggt ggcactttta aacagtgaca cagcgtggcc
    11401 cgtgatctgt tacgccacgg cgatggcggt actggtttcg ttggggctgg tgctccttcg
    11461 gctccgtggg gctgccaccg agaagtcgcc agtcgtctaa ccgacgactg gtagcaggcc
    11521 cgctccgatg cggcgcacta accatcgaaa cctcgtgaat gtcggtatcc tgtctggcag
    11581 gataccgctc atttcccttg ttcagttcat cgccgtcgcc gagcatctga attttcggca
    11641 tgcggccaag gcacttggta tcagccagtc gagcgtcagc gcgcgtgtga aagcgctgga
    11701 ggataacctt ggtgtcctgc tatttgagcg ccatgcgcgg ggcgttcggc taacagacgc
    11761 aggcaggcac ttcatggagc gtgtcacggc gggtgtcgat caactcgatc acgcagtgaa
    11821 gaccgcggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag
    11881 ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
    11941 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
    12001 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
    12061 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
    12121 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
    12181 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
    12241 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
    12301 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg ccagccggcc
    12361 cgagatcatg gggtgggtgc tgggcatcgt ttaccgcgtc attgccacgc acctggtcaa
    12421 gaaagcgggc catacccacc aagtggccaa gacgggcgcg gtcaccctga tccagcgttt
    12481 tggatcggcg ctcaatctga atgttcactt ccacatgctg tttctcgacg gtgtgtatgt
    12541 cgagcaatcc cacggctcag cgcgtttccg ctgggtcaag gcgccgacca gcccagagct
    12601 cacccagctg acgcacacca tcgcccaccg ggtgggtcgc tatctggaac ggcaaggcct
    12661 gctggaacgg gatgtcgaaa acagctatct ggcctcggat gcggtggatg acgacccgat
    12721 gacacccctg ctggggcact cgatcactta ccgtatcgct gtcggttcac aggcggggcg
    12781 aaaggtgttc actttgcaaa ctctgccgac cagtggtgat ccgttcggtg acgggattgg
    12841 caaggtagcc gggtccagcc tgcacgccgg cgtggcggcc agggccgatg aacgcaagaa
    12901 gctcgaacgg ctgtgccggt acatcagccg cccggcggta tccgagaagc ggctgtcgtt
    12961 aacacgaggc ggcaacgtgc gctaccagct caagacgccg taccgggacg gcaccacgca
    13021 cgtcattttc gaaccattgg atttcattgc aaggctggcc gccctggtac cgaagcccag
    13081 agtcaaccta acccgcttcc acggggtgtt cgcacccaac agtcggcacc gggcgttggt
    13141 cacgccggca aaacggggca ggggcaacaa ggtcagggtg
//
LOCUS       DANLUA010000059        13093 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLUA010000059 DANLUA010000000
VERSION     DANLUA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            64..969
                     /locus_tag="PXB44_001195"
     CDS             64..969
                     /locus_tag="PXB44_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5281675.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            971..2212
                     /locus_tag="PXB44_001196"
     CDS             971..2212
                     /locus_tag="PXB44_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5281676.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2209..2841
                     /locus_tag="PXB44_001197"
     CDS             2209..2841
                     /locus_tag="PXB44_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL5281677.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2843..3619
                     /locus_tag="PXB44_001198"
     CDS             2843..3619
                     /locus_tag="PXB44_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL5281678.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3672..5030
                     /locus_tag="PXB44_001199"
     CDS             3672..5030
                     /locus_tag="PXB44_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL5281679.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5092..6432
                     /locus_tag="PXB44_001200"
     CDS             5092..6432
                     /locus_tag="PXB44_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL5281680.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6444..7133
                     /locus_tag="PXB44_001201"
     CDS             6444..7133
                     /locus_tag="PXB44_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL5281681.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            7290..9107
                     /locus_tag="PXB44_001202"
     CDS             7290..9107
                     /locus_tag="PXB44_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL5281682.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            9110..9727
                     /locus_tag="PXB44_001203"
     CDS             9110..9727
                     /locus_tag="PXB44_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5281683.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(9811..10422)
                     /locus_tag="PXB44_001204"
     CDS             complement(9811..10422)
                     /locus_tag="PXB44_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL5281684.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            10617..11525
                     /gene="glyQ"
                     /locus_tag="PXB44_001205"
     CDS             10617..11525
                     /gene="glyQ"
                     /locus_tag="PXB44_001205"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL5281685.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            11605..11865
                     /locus_tag="PXB44_001206"
     CDS             11605..11865
                     /locus_tag="PXB44_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281686.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            11908..12942
                     /locus_tag="PXB44_001207"
     CDS             11908..12942
                     /locus_tag="PXB44_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5281687.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
BASE COUNT         3882 a         2576 c         2961 g         3674 t
ORIGIN      
        1 ttatcttttc tattggttaa tataccgcca tcaagttcat taatttattt gagggaataa
       61 acaatgagtc aaaaatatag cgtagccgtc atcggcttag gagcaatggg tatgggagca
      121 gcaaaatctt gtatcgctgc cggattagac acatacggtg tggatcttaa cccgaattat
      181 ctggaaacct taaaagcagc aggtgctaag aaagtcggca caagtgcggt cgattttgcc
      241 gctgaattag atgcggtgtt gttgttggtg gttaatgcca accaagtgaa tgcggtgtta
      301 ttcggtgaaa acggcttagc ccaacatttg aaacaaggta cggtggtgat ggtatcttcc
      361 accatttctg cacaagatgc aaaaaatatt tcacagaagt taacagattt aggcttggtg
      421 atgttagatg ctccggtttc gggtggggca gcaaaagcag cacaaggtga aatgactgtg
      481 atggcatcag gttccagcga ggcattcgag aaattaaaac cggtattaga tgcggttgca
      541 ggcaaagtgt ataacattgg cgaagaagtc ggcttgggtg caacggtaaa aattatccat
      601 caattacttg ccggtgtgca tattgccgca ggtgcagaag caatggcatt ggcggctcgt
      661 gccggtattc cgcttgattt aatgtatgac gttgtaacca atgcggcagg caattcttgg
      721 atgtttgaaa accgaatgaa acacgttgtt gagggcgatt acaccccact ctctatggtg
      781 gatatttttg tgaaagattt gggattggtg aacgacaccg caaaatcttt gcgtttcccg
      841 cttcacttag ccagcaccgc ttattctatg tttaccgaag ccagcaacgc aggttacggc
      901 aaggaagatg atagtgcggt cattaaaatt ttcagtggta tcgaattacc taaaaaagag
      961 gggaaataag atgttaggca ttattgcaga cgatttcacc ggtgcgagtg acatcgccag
     1021 tttcttaacc gaaaacgggc taaaaacggt gcaaatgaat ggtgtgccga aaactacact
     1081 taacagtcaa gttgatgcga ttgtgattag cctgaaatct cgctctaacc ctgtggaaga
     1141 agcaatccaa caatcattac aagccttaga ttggctgaaa caaaatggtt gctcgcaatt
     1201 ctactttaaa tattgttcca cttttgacag cacggagaaa ggcaatatcg ggccggtaac
     1261 cgatgcccta atggcggcat tagataccga ttttaccgta gtcacaccgg ctctgcctgt
     1321 caatggcaga accattttca acggctattt gtttgtgggc gatgtgctac tgaatgaatc
     1381 tggtatgcgt aaccacccga ttaccccgat gaaagatgcc aacttggtgc gtttaatgga
     1441 tgcacaatct caaggcaaaa ccggcttggt tgcctatgct gatgtgattc aaggggcaga
     1501 gcgtgtgaaa gcccgttttg ctgaattgaa acagcaaggc tacaaatatg cggtagtaga
     1561 tgctgccgat aattcacaac ttgcggtgct tgccgaagcg gttgccgatt ttcaattagt
     1621 cacaggcggt tcaggcttag gggcttatat ggcggcttac cgttcaggcg gcaaaaaagg
     1681 cacgaatgcg tttgtaccaa gcaaaggcaa aaccgttgtg ctttcgggtt cttgctcggt
     1741 aatgaccaat acccaagtgg cggcttacca gcaaaaagcc ccaagttatt tcttagaagc
     1801 ggaaaaagcc attcaggatc cgaactatgt agaaacgctt tatcaatggg tgattgcaaa
     1861 cctagacggt gactttgccc caatggttta tgccaccgtt ccccctgaaa aattgaaaga
     1921 gattcaaacc caattcggcg gcgataaagc cagccacgcc attgaaaata cctttgccaa
     1981 attagccgaa aaattacaag ccgcaggcgt aactaacttt attaccgcag gcggtgaaac
     2041 ttcgagcatt gtggttcagc aacttggatt cagcggtttc cacatcggca aacaaattgc
     2101 accgggcgtg ccgtggttac aagcggtcga aaaaccgatt tttcttgcat tgaaatcggg
     2161 taacttcggt aaagaagatt tcttcagttt tgctcaggag atgtttgtat gacagaacaa
     2221 gaacaaaaga ttgaaatggt caatcttgcc cgctcgctgt ttgagcgggg ctataccgtt
     2281 ggcggtgcgg gcaacctctc cgtgcgttta gatgaaaatc gcattttagt taccccaacc
     2341 ggctcttcgc tcgggcgttt agaggcagac cgcctttccg tattggatat gcagggtaat
     2401 gttctttcag gagataaacc ttcaaaagaa tcggtattcc acttggcgat gtatcagaaa
     2461 aatccggctt gccaagcaat agtgcatctg cattccactt atttaaccgc actttcttgc
     2521 ttggaaaatt tggataccga caacgctatg cgtgctttca ccccttacta tgtgatgcgg
     2581 gtaggaaaat tgccggtcat tccttactac cgtccgggcg acatcaacat tgcccgagag
     2641 ctgggggaac gagccttaga cggcaaagcc tttttactgg caaatcacgg ggtggtagtc
     2701 acaggttcag acttgcgtga tgcggtggat aacacagaag agttggaaga aacctccaag
     2761 ctctatttta ttctacaagg tcaaaaagtc cgttatctca ccgatgacga agtcaatgat
     2821 ctgaaaaaca gaggaaaata aaatgcctaa atttgcagca aaccttacga tgatgtttaa
     2881 cgaagtgccg tttttagatc gttttgaagc ggctgccaac gctggattta aatatgtcga
     2941 gtatttatgg ccttatgatt atccggcagc agaattgaaa gccaaacttg agcaatatgg
     3001 cttaaaacag gtgttgttca ataccgcagc cggagatgtt gctcaaggtg aatggggtgt
     3061 ttctgccatt ccggggcgtg aagctgatag ccatcgtgat attgatttag cgttggaata
     3121 tgccctcgcc ttaggttgtg agaatgttca cgttatggca gcggtcgtgc cagaaggcga
     3181 agatcgtgaa gcctataaac aaacctttat caaaaacgtg caatatgccg ccgataaatt
     3241 caaaccgcac ggcattaacg tcttgcttga agccctaagc cctgaagtga aaccgaatta
     3301 tctgcttaaa agccagtttg atacgctaga aattgtggaa gcggtaaacc gagacaatgt
     3361 gtttgtacag cttgactact tccacgcaca gaatgtggac ggcaacttat ctcgcctaac
     3421 cgacaaactc aagggtaaat ttgcccacat tcaaattgcc tctgtgccag atcgccacga
     3481 accggacgaa ggggaaatta actaccaata tatcttcaac aaactcgatg aaatcgggta
     3541 tgaaggctat gtaggttgcg agtataaacc acgagcggaa accacctcag gactaggttg
     3601 gtttgagcag tataaataac cacgatgatt agcggataaa tacgtgaata caacatttag
     3661 aaggaaactt tatgtcaaat gaaatgttaa ttttgattgg attagcaagc gtagttgctc
     3721 ttctggtcat tatgattaaa ggtaaagtac acccattctt agcattgggg ttagtgagta
     3781 tcgcagttgc cttaactgca ggtattccaa tgagtaaagt gatcccaacc ttgatttcag
     3841 gtatgggaag tactttaggt agcgttgcac tcatagtagg tttgggagca atgttaggga
     3901 aaatcattga aaaatctcac ggtgcggatg tattagcaaa ttggttgtta cagcgttttg
     3961 gtgagaaaaa cgctcccttt gcattaggta tgacaggttt tatattcggt attcctgttt
     4021 tcgttgatgt tggttttatc gtattgctcc cgattatttt tagtgtagcg agacgtttag
     4081 ggggaaatat ccttgtttat tctttgccaa tcgggcttgc gatgttactt gttcacgttt
     4141 tagttccacc tcatccaggt attgtagcgg gtgctcaaaa cttaaacgca gatattggct
     4201 ttgtattggg aattggctta gtagcagcgc tacctgcata tttaatcggt caaaaattta
     4261 ttccgttctt tacaaaaaat aatttcgtaa aagtagtaac ttcaaccgat cttttacagc
     4321 accaaaaaga tttgattaaa gagaaaggca ttgaactacc ttcttttggt aaagtgttag
     4381 caatgatttc tttcccgcta atcctgatta tgttaggtac agtaacggca acaaccttgc
     4441 cgaaagaaca tatcgtgcgt gaagtattca gtatggtagg aagctctccg tttgccttat
     4501 ttgtggctgt ttgtctttct gcttatgtat taggttttag atacggttgg actaagcata
     4561 aattagaaga agtactggag tctgcgttag caccgattgc ggggattatt ttaatcaccg
     4621 gtgcaggcgg tatgttcggt aaagtgttag atgccagtgg cgtaggtaaa gcattagcag
     4681 atgtattagc caacacgggc ttaccgttat tggttctttc tttcatactt tctgcattat
     4741 tgcgtgcagc acaaggttct gcaacggtgg caactattac tactacaact attctagctc
     4801 ctgcagctgt agcagcaggt tataatgatc tgcaattagc acttcttact gcagctattg
     4861 gtgcaggttc tatgacatta tcccacgtga atgattcact cttctgggta tggacgaaat
     4921 tcttcaatgt tcctgtatcc gtaggcttaa gaacttggac gtttttaacg actattttcg
     4981 gtgtaatagc ctttgccgtt gttggcatta tttggatgtt attcgtttaa tatcactctg
     5041 ttactgctta aaatccacct cttatgaggt ggattttttt aggagaaacc tatgctgaaa
     5101 aaaacacttt tatttacgct attactttct cactccctct atgcagagga tatgattcag
     5161 gaaaataaat cagcgaatat tacgttgctt gccgaaatca ccgctcaagg tcaaaaaatt
     5221 aacggcgtgg cattggaata tcctgataat gtcttatcag gcagcgatct tcgccaactg
     5281 tatcaggtca aaacggcatt agatagtgcg gagaaagaaa accgttcggt gctcaaagcc
     5341 tatgtcaata atgagccaaa agtgaccgca cttccgcaag ccggtaaatt tgtgattatt
     5401 gaattggata cggcagataa aaatgcggca acttatagcg taaaagatgc taataaagcg
     5461 ccgatgaaat ttaaatccaa agatgaaaac ggcaacattg tagaaacaga gaaagtacaa
     5521 gcggtcaaaa ttccagagtt ttttgcaaat cgcctaatct attcggtgga acaaaaaggc
     5581 ttgcttaaat taaccagcgg cacaactcta ggcaagactg aatggacaca aaccgctgaa
     5641 cccgacaaaa tcaaaacccc ttatctcgat gattttgtcg caaaacaagt gagcttagac
     5701 cagtctggaa ataaactgaa ctaccgtctc tattcgcctt tgcaaaaaaa tggtgaaaaa
     5761 tacccgcttg tgattttcct acacggttca gggcaagtcg gttcggataa ccttgctcac
     5821 ctgctttcca gtaaaggggc gattgccacc ctgccttatg ccccaagttt tgtacttgct
     5881 ccgcaatacg accaagtatt cgacccattt gataatgccg ccaaagggca aaaaggcggt
     5941 attcactggc aaacggaaaa tcgtatcaat ctcgtgttaa aaatgattga tgaaacccta
     6001 aaagccaatc ctcaaattga taccacacgc atttatatca atggcttatc tcgaggtgct
     6061 gaagggggct taaatctgtt gttggctcgc ccgaatttct ttgccggagc attattaatg
     6121 agcggtcgtg aagcctacac caacgaatgg gtggacggca atgccaccaa aacgaatttg
     6181 gcggcaatta aacatatacc gatttggttt ttccatagta aagaggacaa agtctcgccg
     6241 gtaaaaggtt ctcgcattaa ctaccgcatt ctcaaagatg agttaaatgc ccccaatgtg
     6301 aaatataccg aattcacttt tgaacaagcc ggcgacaacg gcattgtcaa taataaccct
     6361 cataatactt gggacgctgt gtataacagc cctgaggtga ttgggtggtt acttcagcaa
     6421 aaacgccact aaggaggctc actgtgttta tgtatttgtc tcacccgtta gatcctaaag
     6481 atttagcttg gccgggagaa cctgttgttg aggtgactcg ctgcacagac gtaggtgaag
     6541 aaacgccatt tagcagtttt atcacgaaac taccgaatca ttgcggcaca catatggacg
     6601 ctcctcgcca ctttgtgaaa gacggtttaa gtatcaatga attgccgata gagtatttct
     6661 gccataaaga ggtcgcttta attgaaattc ccaaaggaga ggcagaaggg atcaccaaag
     6721 aagatttatt gccttatgtc gatattctct ccaaggtgtc ttttgccttt ttacgaacgg
     6781 ggtttgagaa atatagaacc gagaaccctg ctatttatca aaatgaatgc ccctatattt
     6841 caaccggtgc cgggcagtat ttatctgata ctttcccgaa tttgaaagga gtaggcattg
     6901 atttcttggc actagggtct ccttcttccc gagtgcctga tgcggaaaac cctaaaaatt
     6961 gccatcgagc gattttaggc tactataccg gcaggtttac cactgtgatt gaagatatgc
     7021 acttagcgga actccctaaa aatgccaaca ttaagcagtt ttttaatgca ccgtggcgaa
     7081 ttaaagggtt agattccagt caagtgactt gtattgtgga attacagcaa taaaccacag
     7141 atatattcat tttaggagag gctatttact atagtaagca taactcaatc attaattgaa
     7201 aataattatt atttgatttt aatcaagtca ttcataactt aattttgtta taaatatgac
     7261 cttgtttata gcactatttt gtttcgataa tgattataaa agatacacac tggacatctc
     7321 aacaagcctc tccaaatgct ttcccagaac gacaggcact tatgcctatt tggggtggga
     7381 aaaatattga acgcaatcaa tggcaatctt tatggctgga acaagcacca aatgcgttat
     7441 tacaggatgg tttagcttat ttccatattc cgttttgcgc tagtcattgt attttttgtg
     7501 gtttttatcg caatgcttgg aaggaaaatt atagccagat ttatactgat cgtgtgatta
     7561 gtgaacttcg atatgaatct tcactatggc gaggacaagg taagattcat gccgtttatt
     7621 ttggtggagg aacgccaaca gcgttacata cagaagattt agtacgattg attcgagctt
     7681 gttatcgtta tttgccactc gctgaagact gtgaatttac tattgagggt agaattagcc
     7741 attttgatat agataaggct aaggcttgta ttgaagcagg cgtgaaccgt atttctattg
     7801 gtattcagac ttttaactcc gaaattcgca aacgtttagg gcgcaaacat agtggcgaag
     7861 aggctcaaac ttatttaaaa gccttatcgg aattaaatgc tgtgattgtt gctgatttaa
     7921 tttttggctt gccaaaccaa acagatgata tctggcaaaa cgatattgaa attgcgacta
     7981 gtttaccgat ctcaggctta gatatttacg cctttaataa ttatcctttc ctgcctatta
     8041 atcgcttaat tgaacatgga aaattacctg cgcccgcagg gtttgacacc caaagcttac
     8101 actatgctta tgcagtagag aagttaaaag cacaaggttg gcagcaagtg agcaataacc
     8161 actttgcttt tccaaatcga ggtgaacgca atcgctataa tactttggtt aaatcgaata
     8221 tgccttgttt ggcatttggc tccggtgcgg ggggaaattt tggtgggctg agttaccaag
     8281 tacaatctga tttaaagacc tacttagatt cgcctcttga tcagaaaagc ctttcttttt
     8341 tgagtaaaca tggtgaacat aaagcactgc ttggtgtgat acaacatagc atggaattgg
     8401 gttacttaga tacaacgcct tttgaacaaa atgcgaaagc tgaaaaatta ttgcaacaat
     8461 gggaagcgct tgatttactg aagcgtgaaa aaaatcaagt atatctcaat accagtggtc
     8521 gttactggtc accaacatta attcgtaaac taatgttaac acttcctcaa tcaagtaagg
     8581 ataacaacat gataacgcca ttaactaaag aattagaaaa taaattgcgg acaaatttaa
     8641 aagaaaaccc agggcaaatt ttagaaatgc tggcaggaat gaatcaatgt agcttggaat
     8701 cggtaattaa ctgtctgccc aaagaaatgg taatgcaaac aggtggtgag cgttttgtgg
     8761 aaattatgca ggcaattgct ggttggaatg aagctgtgac ttttattatt catacgcctg
     8821 atgcgattgc agaggtaact gctaaattgc cacaaggtaa aattgggcga ggtttttata
     8881 attttgaaca tggtgtagat ggaggaattc acggtcattt acgctatgaa aattgtgctg
     8941 ctatttactt acttgatcgc ccatttatgg gaaaaagaac ggtggcgtta acttttatca
     9001 atcaaagcgg tgaggcgatg tttaagattt ttgttgggcg tgatgaacaa ggtcaattac
     9061 gagaaaatca gattcaagcc atgcggaaat taattggagg atgctagtca tgattttggt
     9121 ttgtggggca aatgggttgt taggacgagc aatctgtgac aaattaccta gagagaaaac
     9181 gatagccgtc ttacgaaaag atgctatgga tacgtttttc agcgaacgaa atatagcagt
     9241 agaaatagca gatttattag atttggaatc ctgcaacgcc ttgttacaaa aaacacaacc
     9301 ggaaatcttg attacggtag taggaggaaa aaatgaacaa ggtattcgta gcgacaaaac
     9361 agcaaatatt aatttgataa aagcagttga aaaatattca ccaactacga aagtagtttt
     9421 agttactagt gttgggtgtg cagaacaatg ggatttaatt agcccattag cacagcaagc
     9481 tttaggtgaa gcactaaaag ccaagacgga agctgaagct tatttacagc aaacagcatt
     9541 aaattggctg attattcgac ccggtgggtt aaataatgat gaagtaaatg atgagtatca
     9601 gattgtgcgt gatttaccta atgaacggaa aatgtatgta agccgtaaat ctgtcgcaac
     9661 aggcatagtg cgtttactgg atagtacgga aaatcatcaa atcttctcta ttattaatag
     9721 gggatagtat atttcaacga aggcaagcgg gcatatttta tggttatttt gcaactataa
     9781 aacagaccgc ttgtgagatc tttttctatt ttattgagaa ctggatgccg cattcatctg
     9841 caagtagcga agcacctttt tctgggtagc ttcgtccata tcggtaaaac cgaccattcc
     9901 gttaaatact gcaatccatt cgtttgcatc atgcatttta ggatcacgtt gtttatggca
     9961 cacgctgcat tctgttttat aggtattttt ggtgttcgcc caaaagttag tgagatcttg
    10021 ggtaaattcg gcattttcca cccacacgtt cattttcact ttttgccata ccaagccggt
    10081 gttcggatct tccttgctct ctacaatctc taacagtggg ttgttgctga taaaagattt
    10141 ctcaaatacc gcatcggtaa tatttttact gaaagaatga taccaaatcc gcccgaaacc
    10201 tttgcttttt cgccataaat tcagctcaat ttcagcggct tttgcggttg tttgcaccaa
    10261 tttaatttct gctccggctt ctagctgccc gattttctgt gtcaaatttt catcggcaaa
    10321 taaagggatt gtttccgtgg tgtaactgtt tgaaccggca aaacaaaatg ttggcatgat
    10381 gaatgccaag aaacagaaag cgattttcga taatgatttc atcataaacc ccacaaatta
    10441 gatgagaaat taggttatat gttacacaaa ttgagcaaaa aagcgttgat ttacatcata
    10501 aaactgattt tatgtcgatt tcttaatttt ttgcaaaaaa atcccaaaaa atgactgctt
    10561 acaagctata ataaccagta tttttcgacc atttttcaaa ttcaagagaa aataaaatga
    10621 gtacaaaatt caacgtaaaa acctttcaag gaatgatttt agccctgcaa gactactggg
    10681 caagcaaagg ttgcaccatt gtgcagccgt tcgatttaga agtgggggca ggcacctcgc
    10741 acccaatgac ctgcctgcgt gcgcttggcc ctgagccaat ggcgtttgcc tatgtgcaac
    10801 catctcgccg cccgacagac ggtcgctacg gcgaaaatcc gaaccgttta cagcactact
    10861 accagttcca agtggtgatt aaaccttcgc cggataacat tcaagaactt tacctcgaca
    10921 gcctgaaaat gctcggtttc gacccaaccc aaaacgacat tcgttttgtg gaagacaact
    10981 gggaaaaccc aacgcttggg gcgtggggct taggttggga agtgtggctg aacggaatgg
    11041 aagtgaccca attcacctac ttccagcaag tcggcggctt ggaatgtaaa ccggtgacag
    11101 gcgaaatcac ctacggctta gagcgtttgg cgatgtacat tcaaggcgtg gacagcgtgt
    11161 acgacctcgt gtggtctgac ggcgaactcg gtaaaaccac ctacggcgac gtattccacc
    11221 aaaacgaagt ggagcaatcc acctacaact ttgaatacgc tgatgtggac ttcctgttcc
    11281 aatgcttcga gcagtacgaa aaagaagccc aatacctgtt aggcctagaa aaaccattgc
    11341 cattgccggc atacgaacgc attttaaaag ccgctcacag ctttaactta ttagatgctc
    11401 gtaaagcgat ctcggtaact gaacgccaac gctacattct acgcattcgt gccttaacca
    11461 aaggcgttgc tgaagcgtac tatgccagcc gtgaagcgtt ggggttcccg gggtgtaaaa
    11521 aataatcgta gggacgccac gcttggcgtc cgtcaataaa ttacaaaacg gacacgaagc
    11581 atcgtgtccc tacaaggatt tacgatgaaa ccgattttat acttcgccga ttggtgtccg
    11641 gataccgccc catttgtggc agaactagac cgcttgggca ttgaatatga gccgtgcgac
    11701 atcaccaaag gaggctcaac acttaaacca tttttgaaac tgcgagacac gcattctgcc
    11761 tttgaccacg ccaaagccaa cggctatatt ggcattccgg cattgctgat cgaaggcgaa
    11821 aaagtcgtgc tggatttggc tgaattagag ggtattttcg gctaacaagc ggttgaattt
    11881 ggcggaaatg ttgcaaagga gccaataatg aagacagtaa tagcagataa ttcggctgaa
    11941 tttgtgaatg aaatttcaca acttattcaa tcgagtaagc aacgaatgac cgttgcggtt
    12001 aatgcagaat taaccttgct ttattggcat atcggaaaac ggatcaatga ctatattttg
    12061 cagggtgaac gtgcggaata tgggcaagaa gtggtgaaaa atcttgctca aagtctgacg
    12121 gagcaatttg gtaaaggttg gagtaaacgc catttaaatt atacaatgca atttgctaca
    12181 actttcccta atttggaaat tgtgcacgca ctgcgtgcac aattaagctg gacacatttt
    12241 aagcagatta ttcatattga tgatccaaca aagcgagggt tctatgcaac aatggctgca
    12301 caggagcgtt ggagtacaag gacattagaa gatcgcattg gctcattatt gtttgaacgc
    12361 acggcaattt ccaaaaagcc cgatgaaacc attattgctg aattgagttt gctacgtgaa
    12421 aaaggcgaat ataatcagtc aatggtgttg aaagatccct atattttgga ttttctcgac
    12481 cttaacgacc gttatatgga acgtgatctc gaagatgcga ttctacgaga gatcgaacag
    12541 tttttactgg aacttggggc gggttttacc tttgtcgccc gccaaaaaag gattcagatt
    12601 gataatgatg atttctatat cgatctgctg ttttataacc gtaaactcaa acgcctgatt
    12661 gcgattgaac taaaaaccga aagcttcaaa cattcccaca aaagtcaaat ggaactctat
    12721 ttggcgtggc tggcgaaata cgagcaagaa gcagacgaaa acccaccgct tggaatgatt
    12781 ctctgcaccg gtaaaaagca ggagcaagtg gaattgttgg atttgaaaaa ttccgatatt
    12841 catattgcgg aatatatgac cgtgttgccg agtaaagcgg tattggaaca acggttgcat
    12901 ttggcggtgg aaagggcgaa agagcggttt ttactaaaat agcaagcggt taaaatcagc
    12961 ggaaatgttg caaaatgaaa gtatgatgga aacgatttgg ttgcatattc cgttggtggg
    13021 tggttagaga ttttcaaatc tcccccctcc ccctctttgg ctaaagaggg ggcgatcatt
    13081 tagtgagtaa ttt
//
LOCUS       DANLUA010000060        13021 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLUA010000060 DANLUA010000000
VERSION     DANLUA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13021
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            184..561
                     /locus_tag="PXB44_001208"
     CDS             184..561
                     /locus_tag="PXB44_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL5281688.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            650..1237
                     /gene="yihI"
                     /locus_tag="PXB44_001209"
     CDS             650..1237
                     /gene="yihI"
                     /locus_tag="PXB44_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL5281689.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1251..1718
                     /locus_tag="PXB44_001210"
     CDS             1251..1718
                     /locus_tag="PXB44_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL5281690.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1718..3085
                     /gene="hemN"
                     /locus_tag="PXB44_001211"
     CDS             1718..3085
                     /gene="hemN"
                     /locus_tag="PXB44_001211"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL5281691.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3159..5492
                     /gene="lptD"
                     /locus_tag="PXB44_001212"
     CDS             3159..5492
                     /gene="lptD"
                     /locus_tag="PXB44_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL5281692.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5634..8273)
                     /gene="ppc"
                     /locus_tag="PXB44_001213"
     CDS             complement(5634..8273)
                     /gene="ppc"
                     /locus_tag="PXB44_001213"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL5281693.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMFAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            8398..9366
                     /locus_tag="PXB44_001214"
     CDS             8398..9366
                     /locus_tag="PXB44_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL5281694.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
     gene            complement(9415..9585)
                     /locus_tag="PXB44_001215"
     CDS             complement(9415..9585)
                     /locus_tag="PXB44_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5281695.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
     gene            complement(9586..10167)
                     /locus_tag="PXB44_001216"
                     /pseudo
     CDS             complement(9586..10167)
                     /locus_tag="PXB44_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(10386..11126)
                     /locus_tag="PXB44_001217"
     CDS             complement(10386..11126)
                     /locus_tag="PXB44_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL5281696.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
     gene            complement(11116..12039)
                     /locus_tag="PXB44_001218"
     CDS             complement(11116..12039)
                     /locus_tag="PXB44_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL5281697.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            complement(12140..12427)
                     /locus_tag="PXB44_001219"
     CDS             complement(12140..12427)
                     /locus_tag="PXB44_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281698.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            complement(12453..12893)
                     /locus_tag="PXB44_001220"
     CDS             complement(12453..12893)
                     /locus_tag="PXB44_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5281699.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
BASE COUNT         3878 a         2553 c         2738 g         3852 t
ORIGIN      
        1 tggcatagta gttccttttt gatgtatcaa tactaaaaat aacctgtatt attctagcag
       61 atttctgggt aatcgaggag ttgataacat ttaaaaatta gcgttttgca aaattttcca
      121 aaaaaagacc gcttgttagg ctataatagc caaccaaaca gaccaattca aaggagaatc
      181 ggtatggaat atcaatttac ccatagtatt cacggggtgg tggcaaaatg ttcgatggat
      241 cacgaggcgt ttgctcgttg gttgaatgcc gaaataacgg aaaatcctag ccagttagcc
      301 cctattttcg ctgaaataga gaagtgtcga gccgcatttc cgaatcatta tgaatgtgtg
      361 tttgaagggc gggagtatag tctctatttt gattatgatg aagtgatggc aaaagcaaac
      421 aatttagatg ctgcttttga tgatgaggaa atggaagaag gctttcaatt ttacaatgaa
      481 gagagcatcg cattttgtgg cttagatgac tttgagaaat ttttgaaagc ctaccaacat
      541 tttgtgcaaa cttatcattg attgaaatga ttaattgaaa ccaaagctat taagggaatc
      601 taattccttg tagaatagcc gtttatagca attttaagat attaaattta tgagtcgtac
      661 taaaaaaaca cgtcgtatta ccgacattat gccggcaaga aagaccgata aacctaaaca
      721 gcccatgcct aaattaggtg gggggaaaaa tcgcaaactg acccgttatg agttagatgc
      781 tcaagcccgt gaggaaaaac gtaagcgtaa acataaaggc ttgccgacag gctcacgcaa
      841 tgctgatccg gcagagcaga aaaaagcagt ggtgaaagag gtgaaagatc cgcgtatcgg
      901 tagccgtaaa aaagtgccgt taatggtgga atttgtgaac cagccggaaa aagggcggac
      961 gattaaggct gtgccggttg agccaatcaa accaacgctt tcaccggaac aagagctgga
     1021 gcagttagaa aataacgaat gtttaaatca gttgttagat gatttggagg cgggcaaaac
     1081 cttatccgca gaagatcaga aatttatgaa tgaatgttta gatcgcattg atgaattaat
     1141 gactgagctt ggcatcgaat atgaagatga agagggcgat aatggcgatg cgttattacg
     1201 ccaatttgaa acgatggata ttaataaatt ccgctaggta gccgattatg atgctaagaa
     1261 tattcctgat ggtgcttgcc gctttgattt taatttcgct gggcgggtat gcactgcact
     1321 taatgctgaa agtgcgagct cagaaaaagc acgaacaagc actgattgaa caggcaaagc
     1381 aagcacaaaa agatcgttat attcggattt tagacagcat tgaagttatt gctcgggcaa
     1441 tgatttcaga acagtgcgat ttttcggaag gcgttttacg tttaaaaccg ctattagatg
     1501 ttctcggcag aaaattaagc gactatacgg cgatgtgggc gttgtatggc gtggtagaaa
     1561 atatgccgat tttagatgaa cgcaaaaatt taaaacgtaa cgaaagaatg aagttggatt
     1621 tagagcggga agcaaaagaa gcggagcttg aatcagacat caaaaatgag tgttaccaat
     1681 tattaaaaga tattgaagaa atgagaaagg caatctaatg tcagaaatta tttgggattt
     1741 agcccttatt caaaaatata accattcagg gcctcgttat acctcttacc caacagcgtt
     1801 ggagtttaac gaaaattaca ccaatgaaga ttttattcgt gctgcagctc gttaccctga
     1861 gcgtccgctt tcgctttatg tgcatattcc gttttgccat aaactgtgct atttctgtgg
     1921 ctgtaataaa gtgattactc gtcatcgcca taaagttgaa atttatttag attatttaga
     1981 gcgtgaaatt aaaactcgcg cacctctgtt tcaaaatcgt ttagtgaccc aaatacactg
     2041 gggtggcggt acgccgactt atttagacga agatcaatcg gcacgtttaa tgcaaatgct
     2101 acgagccaat tttgatgtga gcgatgatgc ggaaatcagc attgaaatgg atccgcgtga
     2161 aatcgaatta agtatgttgg atcacctgcg tgctatcggt tttaaccgtc tgagtatggg
     2221 tattcaggat ttcgataaag aagtgcaacg tttggtgaat cgtgagcaag atgaagagtt
     2281 tatttttgcc ttgatgaaac gggcaaaaga acttggtttt gtttcaacta atgtggattt
     2341 gatttacggc ttgccaaaac agaatgtgga aagctttatg tacaccttaa aacgtgtggt
     2401 agagctaaac cctgatcgta tgagcgtatt taactatgcc cacttgccaa gccgttttgc
     2461 tgcacaagtg aaaattaaag aagagttgtt gccggctccg gaaaccaaat tgacgatttt
     2521 gcaaaattcc atcgaatttt taggcaaaaa cggttataag tttatcggta tggatcactt
     2581 tgctaaacct gatgatgagt tagctattgc acaagaaaaa ggcattttac accgtaactt
     2641 ccaaggctat accactcaag aagagtgtga tttattggga atgggcgtat cggcaatcag
     2701 cttactcggc gatacttacg cccaaaaccg caaagaatta caacaatact atgctgatgt
     2761 tgaaaccaac ggtattgcgt tgcataaagg cattgcgtta acccaagatg attgcattcg
     2821 ccgtgatgtg ataaaagcct tgatttgtaa tttcaaatta gattttgcca ccattgaaaa
     2881 gcaatatggt attgatttta aacgttattt tgccgaagat ttagctttct tagcgccgtt
     2941 ggcggaagat gaattattaa cgatgaatga cgacagcatt gtggtttccc cacgagggcg
     3001 tttattgatc cgtaatattt gcttatgctt tgatgtctat tcaagacaat tagcgagaag
     3061 acagcagttt tcccgtatta tttaatgagc aagcggtcga ttttggtaaa aattttgcaa
     3121 aaacagaccg cttgttcccg gcttataaag gaaaaatgat gaaacagcgt tatagcttac
     3181 tttctcttgc tgtgatggca gcttgttata gcaacagtgc cgcagctgat ttaaagcaac
     3241 aatgtttact tggcgtgccg cattttcagg gcgaagtggt agagggcgat caacttagaa
     3301 tgcctgttac cattgaggct gacagtgcgg tcattaacca gcctcgtgat gccacctaca
     3361 ccggtgatgt aacgatcaaa caaggtaacc gttcgatttg ggcggatcag gtgcgggttg
     3421 agcaagatgg cgaaaatgcc cgaatggcgt atttgcaggg taattttaac taccaagata
     3481 atttgattca ggcaaccggt tcagatgcga ccatcaatct gctttccaaa gatgcgaatt
     3541 tagctaatgc taattaccaa ttaattggca gacaagggcg tggcacggca gaatccggtg
     3601 aattcggcaa cagcaaacac acgctgaaaa atgccagttt cacttcgtgc ttaccgaatg
     3661 ataattcgtg gcaaattgaa gcgaatgaaa tgattcagca cgttgatgaa gaatatgcgg
     3721 aaatgtggca cgcccgtttt aaagtgctgg gaatgccggt attttattcg ccttacttac
     3781 aatttccgat tggcgaccgc cgccgtagtg ggttattaat tccaagtttt gagcgttcaa
     3841 gcaaaaacgg ctggacatat tcgcaaccgt tttattggaa tatcgcaccg aatatggact
     3901 cgaccatcac tccaacttac tattcacgcc gtggttggca aatcagcccg gaatttcgtt
     3961 atttaacgaa aatcgggcaa ggcttagtgg caaccgaata tatggcgaaa gatcgtttaa
     4021 gcgagtacca accgggaaat gatgatcgta aacgctattt attccactgg cgtcacgcaa
     4081 tgagtttttt aaccgattgg cgtttctcgg tcgattacac caaagtgagc gacagacgtt
     4141 atttctctga ttttgattcg gattatggta gcagtactga tggctatgcg acgcagaatt
     4201 ttaaattagg ctactatcag ccgaattata atatctcaat ttcaggtaaa aaattccaaa
     4261 ccttcgatga gttggatatc gggccttatc gcgttctgcc acaaattgat tttaactatt
     4321 ataaaaacga tctaattaaa ggcggagatt tcaaattctt tgcccaagca gcccgttttg
     4381 aaaatgatag taaattaatg ccaaaagcgt ggcgtttcca cgctgagcca acactgaatt
     4441 tcccattagc gaaccgctat ggtagcttaa atttagaaac caagctctat gcaacctact
     4501 atcaacaaac gaaaggtagt gagagtacgg aagaaattaa gaaaagtgta agtcgtgtac
     4561 tgccacagat taagctcgat tttaaaaccg tgttggaatc cgataaacag cttttctctg
     4621 gctatactca aacgcttgag cctcgggtgc aatatattta tcgcccatat aaggatcaaa
     4681 gcgatattgg ctcgaaagca catcgaagtg tgagcttagg ctatgactct gcattattgc
     4741 aaaacgacta tttctcactg tttaatgaca gacgttatag cggtttagat cgtatttcgt
     4801 cagcaaattt agtcaccgca ggtggcacaa cccgtttctt taaagaggca accggtgaag
     4861 aggtgtttaa tttcagctta ggacaaactt attatataag cccgtctaaa attgatgata
     4921 cctcagataa cagcactaaa ggtcatacga cttcatgggc agcggaatct aactggaaat
     4981 tccataagaa ttggaattgg catggtagct atcaatatga tactcgttta aaccaaacgg
     5041 cgttagcgaa tatgtcattg caatacaaac cacagtcaga caaattgatt cagcttaatt
     5101 accgctatgc aagtaaagat tacatcaatc aaaacttgaa cacgaataag tatgggcaat
     5161 ccattaaaca agtgggcggt gtgctaggtt gggaattaac cgataaagtg tcggtaatga
     5221 ccagttatta ccacgacctt gcattgaaaa aaccggtgga agggcaaatt tcgcttaact
     5281 acaacacttg ctgttgggcg gcaaatgtgt atgtggctcg tcgcttagtt tcaacgccgg
     5341 taggttcatc tgatacgatc aatgatttct actatgacaa taaattcggc attaactttg
     5401 agctacgctt tggcacaaac tacagcaatg gtgtaacccg tatgctaaaa cgaggtatta
     5461 ttccttacac cgagccgtat agcattaatt aacaaagtaa aaacaaataa gtaaaaatac
     5521 aaatcggggc gaaaaatatt tgccccgatt tttttatatt tggctgataa gcggttaaat
     5581 tctgttggaa ttttgcaaat cttccgctaa attcaaccgc ttgccatcga aacttaacct
     5641 gtatttcgca tacctgcggc aatacctgtg atggtgatca tcaatgcttg ctctagtaca
     5701 ggatcagcat tttcaccttg tttgcgaatc ctatctaaca attctacctg aagtagattg
     5761 agcggatcgg tataaatatt gcgtagtgca atcgattctg cgatccaagg taaatcggcc
     5821 attaattgtc catcgtgagc cagtgcaagt acggttttaa gatcagcctg tagttgcgta
     5881 cgcaacatat tgcctaaacg gtgtagtttt ggctcgacta ggcgttggtc gtaatattcc
     5941 gccagccaaa tatcagcttt actaaatacc atttccagca ttccgatacg ggtcgagaaa
     6001 aacggccatt cgttacacat ttcttttagt aaggtttcat taccttcatc aatcaactgt
     6061 tgcaatgctt gccctgcacc taaccaagca ggtagcatta agcggttttg catccaagcg
     6121 aaaatccaag gaattgcacg caaactctcc actccgcctt ttggattacg ttttgcaggg
     6181 cgtgagccaa gcggcagacg ggaaagttct tgctctggtg tagcagagcg gaaatacggt
     6241 acgaagtcta gctcgccacg caccatatca cggtaaattt ggcaagaaat atcggaggct
     6301 tttgctagca catctcgcca ttgctgcttt ggctccggtg gtgggaaaat attcgcttct
     6361 aaaatcgcac tggcatagag ttctaaactt tcaactgcca cggcaggtaa gcctaattta
     6421 aagcggatca tttctccttg ttcggtcact cgtaagccat ttttgagtga gcgaggtggc
     6481 tgagaaagta aggctgcgtg agccggtgcg ccaccacgcc cgatagtacc accacgaccg
     6541 tggaataagg ttaattcaat attatatttt tcacataaat taaccagagc ttcttgcgcc
     6601 cgatattgcg cccagccggc agcaaacatt ccggcatctt tggcggaatc agagtagccg
     6661 atcataatca tttggtaatt gttgatgacg ccacgatacc agccgatatt aaataaatcg
     6721 gtcatcactt tttcactatg atctaaatcg cctagcgttt caaataatgg ggcaaccgga
     6781 atagtaaatt tgcaccccgc ctctttgagt aaaaggtgaa ccgagagtac atcagaggcc
     6841 tctcttgcca ttgaaatgac ataggctgaa atgacacctt caggttgctc ggcaacaatt
     6901 ttacaagtgt ctaacacttc tttagtttca gggcttggag tccagttggt tggaatcaat
     6961 gggcggcgag aactgagttc acgcactaag aatgcctgtt tatcatcttc cgtccagtga
     7021 gcataatcgc ctaagccgat atagcgggta atttctgcaa tcgccatttc gtggcgggta
     7081 ctttcttggc gaatatctaa gcgtgataaa cctaaaccaa aacagtttat acgacgtaag
     7141 caatctagta atgcaccatt tgcaataatt cgcataccac aggcttgtaa tgactcatag
     7201 caatcaaaaa gcggttccca caattgctga tctgaagtta aaatgtcact ttcatcaata
     7261 gttgaaattt taccttccaa taattccgca tagtaaattt cggtattaat taatttctgg
     7321 cgtaatactt ttagtaatac gcgatatggc tctaaatggt tgccatattt agcacgaaat
     7381 gcaggggtac actgtgtaat cgataattct tcagcaagat ttttaatatc ttgtaagaac
     7441 aattccgtgg ctttccaacg gttcattctt aatacacggc gagttgtttc tgccgtgacg
     7501 aacgggttac cgtcacggtc tccgcccatc caagaagaaa aacggacagg ggctaaattt
     7561 accggatatt gcacaccaaa gtgtttttct aaatagaagt taagctgacg gcaaaattcc
     7621 ggcacggctt tccataaact attttcaatg actgccatac cgcctttggc ttcatcaaca
     7681 ggggttgggc gttgggttct gatttcatta gtatgccaag caagggcaat cagttgcatt
     7741 aaacggcgct tgattttata gctttctgcc tcagttaaat catcatgttc caacaaattc
     7801 aaacatttat tgatttcaac gtgtttatgc cccagcgaac ggcgggttac ttccgttggg
     7861 tgagctgtta gcactaaatc aatcattagg ctttccacgg ttttaatcac tttttcaacg
     7921 gggatatttt gtgattttag acgaaggaac agagagtcta atgagcgact accaagtgat
     7981 tcatcaatat gatggcgaga aacagtttga tgttgttcag caatatttgt tagatttaga
     8041 aaatggctaa atgcacgtgc aacgggtaag acatcatcgt tagaaatatt ggctaattta
     8101 tctaatagct gttcacgggc gttttcatcg ccggcacgag aattgcgaga taacacacga
     8161 atgctttcga tgagttctag aatgtaattt ccttgcgcat cacgaatcgt ttctcccaaa
     8221 aattcaccta acatatggat attatgctga attgttgaat attgctggtt cataaaaaat
     8281 tcctatggta aacataaatt aaattaggtc taaatatgcc gaaaatcgct gaaaatagca
     8341 aatagattta atttaatttt tgaattaaat gataaaacgc aaaaaatagc tgtcaatatg
     8401 tgtttaaaac tgttgttcgg ttttgataac ttgcctgttg tttatgtgct attcactgtt
     8461 attcaagttg gcttatccat taatggtttt aaaggtatta aatggttgga aaatgtttct
     8521 tgtgtgttcc tcatcggtat tcttgcttat atgctttatg ttgtgaatac aaaattctct
     8581 gtagaaatag gcgagcgctt tgccaatatt gaaggcactt ggggaatgcc attctgggct
     8641 gcaactacag cattcttggg tatttattca acaatgattt taaatgcttc cgattattct
     8701 cgccacgtta aaaaaggaac aaccccaaca gcaacaggtg tgatttatgt attatcaatc
     8761 ctccctgtaa ccttatttat gggtttaata gggttgttag ttacggcagc tacaggcaat
     8821 agtgacccaa ttgaagtatt tgcaactgca atggataata aatttttaac agtaataacg
     8881 ttattattca ttgcttttgc acaggttacg acaaacgtgt tgaataatat tgttccgcca
     8941 tcttacattt taatggattc atttaaaatg aaatggtcgc atgccaccat tttagtgggc
     9001 attctttcta tatgtgtaat gccttggaaa ttggtaactg ctgaatcagc agatggatta
     9061 tctttgttta ttaaaattta ctcggcattc ttaggcccta tttttgcggt aatggtcgtc
     9121 gattactaca tcttaagaaa acgtacttta aacgtaaatg acttatataa taaagaaggt
     9181 attttcaaag ggattaactg ggcagcaatt attgcgattg ctatcggttc gttttgctca
     9241 ctgattgtgg tggatctctc ttggtatgtc agtttaattc catcaggctt gagctactat
     9301 attttgatga gggtgttaaa aagttccgaa gctttccgta aagggacagt gctagaacat
     9361 caataagatg acaagcggta aaatttatta aaaattttac cgcttgcttt tacgctaaga
     9421 gttaaattct ttcattagct tatctacggt tttgcagttt tggctaaagg ctctgcaacg
     9481 agaacaaagc attaaatgaa cttgcacatt aacacgttgt tttaatgtca gtttttgttc
     9541 ttgggacaga gaaactaatt cggtaatttg cttacatttt aacatttaac ctccaaacca
     9601 tttttgactt aaacagacct gtaactgcaa tcttgcccga tacagtaagg tatgcacatt
     9661 tgctggtgaa atctcacatt ctgcgcaaat ttcttcggtt tccatttcca aatgggaacg
     9721 catcataaac acccttgctt gatttgccgg cagattattt aggcaaaatt caaatacttg
     9781 ccagaattga gcggaatagg tatggctttc ggtgttcttc cactcattag attgataagc
     9841 atctttatcc caaccgccat tttcgttaaa caatttatcg gcaagatcgt gatcgtcttc
     9901 ttccatcagc tcagatactg ccaccaattt gcctttggct ttcactaaat ctacgatctt
     9961 gttttttaaa attgcaaaaa tctacgtttt taacgcactt tcacctttga agctgtcggc
    10021 atatttataa gcgctgacaa acgcctcttg caccacatct tcggcaagtt cgttattttt
    10081 taactgtaaa aaggcaaact tcaccatttg cagacgaata tcttctagct gcgaggggga
    10141 aatttgccgt aaagccggtt ggctcattgc gtgatccttt ggtaaaaaat tgagagaaac
    10201 taaccgcttg tatcctattt cataaggttt tacccaactt aggggatatt ggtatgaagt
    10261 atttttaaac ctaacatact aatcgtaggg gcgggtcctg tgcccgcccg ttatcttaat
    10321 attagtcggg cgggcacagg acccgcccct actatgtatg tcagtacata tcacatacct
    10381 tatcctcatt caggatagac aggataaatc acctctgctt caacccattt ttcccaaagt
    10441 tggcgtaaca aggcttttag ctccagattt tgctcggtaa actcgctgag ttctgctagt
    10501 aagccattca gggaatttgc cccctcttgc aacgagctaa gcaggaagaa atccagctct
    10561 tccaagcggt tgtaatagat gccaaattcc gtatctcgcc aaatcagtac gttggtcggc
    10621 tcaaagtcaa actgtttgaa ctcgctacgg ataaagttta ccgcatattg acgcagctca
    10681 gctgcaccag aaaactgcat tatagtatcg ttatcccatt caaattctga tggtacggtt
    10741 gccattgata cctcagccag taattgagca ttttcaaagt ccatcaactc aagcaagtgt
    10801 ggtggcatac agccattttc tcggcaaaaa ttgagaaatt cgccagcaat atcttggaaa
    10861 aagggcgaat gagccttacc ttctcgtata aatttttctt taacggcaac ccattcttca
    10921 ggacttagat gttgagggag ctgtacataa caacggtcga taaaaccgag agtattattg
    10981 cgaaccaagc gagaatagac gccaagccgc tgtggagaga tattaccact catcagcttt
    11041 acatcgttat ggcgaattgc attcgcaaac tcggcttgaa tctctttgag taacggtttg
    11101 tgttcttcaa cgagtttaag cggcattttt tagcctcgtt tttgcatatt ttttctgtaa
    11161 gctcataatg tgctgtactt cactaatcag ttcttgaaac ggtgggaagt tgaaatcccg
    11221 ttctaacagt gttggtggaa tatgcggtag gtgttcatag gtgtactcta gtaaatccca
    11281 cacagttttt tgtacagctt ctccgtgtgt atcgaccagt aaagttggca gatgacgtag
    11341 ttcgcctttt accatattaa atgattcacc ctcggtcatt ttaacgtctt ccgccgcacc
    11401 gtgatcttca tcgtggcctg caatatgaat ataattgaca cgagatttat ccacttgatc
    11461 aataaataca aatggatcaa gcaagccgtg attgacacca ttaacataaa tattattcac
    11521 atcaaggtga atgccacaat ccgcttcttg ggcaattgca ttgagaaact ctacctcatt
    11581 catcgtactg gttggtgaat gcagatagta ggacgtattt tcaagggaaa tttgcatacc
    11641 taaataatct tgcacttgtt taatgcgatt tgccacgtga tgtacggctt catcggtaaa
    11701 cggcattggc agcaggtcat ataaatgccc ttcacattcg caatagctta aatgatcaga
    11761 gaagaaaaag gagttatact gctgctgcat attcttgatc ccttttagta gcgtcatatc
    11821 taaaggcgct tgcccgccga gagagaggga taaaccgtga ataataacag ggattttttc
    11881 agcgacttta tcgaagttat accgagcctg accgcccatt ttcagccagt tttctggggc
    11941 tacttccata aaattaatta tattgttatc tgtgagagat aaaaatccgt ccgctaggtc
    12001 acggcgatag cctagaccgg caccagtaaa ttttaacatc gtcttgttcc tttaatttgc
    12061 aaaaaatcag cggaatttaa ccgcttgtag attgtgttta taagaggtta ggtgcgttta
    12121 gaaaacgcac cttcgctact tacttagaag ctccacactt gccttcaccg catttacctt
    12181 ctgccgcttt tggtgccgtt gctccacatt tcccttcgcc gcatttacct tcggttgctt
    12241 ttggctcaga agaggttgct ccacatttgc cttcgccaca tttaccttct gtagctttgg
    12301 tttttacaca tttgccatct tttacttcca tacaagcatc tgctttcact gatttagttt
    12361 cagcgtttac agtcgcggtt gccatagtta atgcaccagc taatgccatt aaagttgctt
    12421 ttttcattaa aaattctcca taaattaagg gattaaagga atttcaacca gcgtttggtt
    12481 ggggattttt tctgtaacag aaaatcgaga gagagcgagc cagcaccttg taataaaagt
    12541 ggtactaaca tcactaaata aatgagtgcc attttatagc cattgttgca gacattgtag
    12601 ccattgccag cgtgaacagc ataccacgct accgctgtta agatcattaa tcctaacgca
    12661 ctgaagcggg tgaacagacc caaagtgagt aaaataggca gtaagatttc cgctccgcta
    12721 gcaatagccc agctaagatc ggctggtagt acgttaaagg ggaatgggaa gctctcttga
    12781 attgaggcaa accaattttc accgtttagt tttgataacc ctgcttcaaa aaattcataa
    12841 gcaagaaata gacgtaatac aagaaagccc aagccgtttt tacaagctgt cattgagcaa
    12901 gctgactgag ttgaaaatga tgacgaaaat gatgacataa aaatgctcct aactaaaaat
    12961 gtgattatta gacagaataa gtatacgttt attacaaaaa atgtgtgaaa aattataacc
    13021 t
//
LOCUS       DANLUA010000061        12953 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLUA010000061 DANLUA010000000
VERSION     DANLUA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            40..1185
                     /gene="lldD"
                     /locus_tag="PXB44_001221"
     CDS             40..1185
                     /gene="lldD"
                     /locus_tag="PXB44_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL5281700.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1239..1508)
                     /locus_tag="PXB44_001222"
     CDS             complement(1239..1508)
                     /locus_tag="PXB44_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281701.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(1583..1969)
                     /locus_tag="PXB44_001223"
     CDS             complement(1583..1969)
                     /locus_tag="PXB44_001223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281702.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            complement(2222..3052)
                     /gene="fghA"
                     /locus_tag="PXB44_001224"
     CDS             complement(2222..3052)
                     /gene="fghA"
                     /locus_tag="PXB44_001224"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL5281703.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(3132..4190)
                     /locus_tag="PXB44_001225"
     CDS             complement(3132..4190)
                     /locus_tag="PXB44_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL5281704.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(4377..5312)
                     /locus_tag="PXB44_001226"
     CDS             complement(4377..5312)
                     /locus_tag="PXB44_001226"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL5281705.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            complement(5592..5903)
                     /locus_tag="PXB44_001227"
     CDS             complement(5592..5903)
                     /locus_tag="PXB44_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5281706.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(5910..6302)
                     /locus_tag="PXB44_001228"
     CDS             complement(5910..6302)
                     /locus_tag="PXB44_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5281707.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            6434..6994
                     /locus_tag="PXB44_001229"
     CDS             6434..6994
                     /locus_tag="PXB44_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL5281708.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(7060..8049)
                     /locus_tag="PXB44_001230"
     CDS             complement(7060..8049)
                     /locus_tag="PXB44_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL5281709.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGDVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(8263..9180)
                     /locus_tag="PXB44_001231"
     CDS             complement(8263..9180)
                     /locus_tag="PXB44_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5281710.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(9316..9975)
                     /locus_tag="PXB44_001232"
     CDS             complement(9316..9975)
                     /locus_tag="PXB44_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5281711.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(10160..11224)
                     /locus_tag="PXB44_001233"
     CDS             complement(10160..11224)
                     /locus_tag="PXB44_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL5281712.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            11315..12223
                     /locus_tag="PXB44_001234"
     CDS             11315..12223
                     /locus_tag="PXB44_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5281713.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(12293..12844)
                     /locus_tag="PXB44_001235"
     CDS             complement(12293..12844)
                     /locus_tag="PXB44_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5281714.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
BASE COUNT         3631 a         2912 c         2822 g         3588 t
ORIGIN      
        1 atagccgcac ttttttctta atgatttatg aggttttaaa tgatcatttc atctgcaaac
       61 gattaccgcc gtgcggcaaa acgtcgtgtt ccccctttta tgttccatta tgcggatggc
      121 ggttcttacg ccgagcagac cttacgtcgt aatgtgagcg atttggaaga catcgccctg
      181 cgtcaacgtg tgttaaaaga tatgtcgcaa ctagacaccg gcatcgagct gttcggcgaa
      241 aaactgtcta tgccggtgac cctcgcccct gtcggtgcat taggaatgta tgcccgccgt
      301 ggcgaagtgc aagcggccaa agccgcagat aacaaaggta ttccgtttac cctttccact
      361 gtctctattt gcccgattga agaggttgca ccggcaatca aacgcccgat gtggtttcag
      421 ctctatgtgt taaaagatcg gggctttatg aaaaatgcat tggaacgagc caaagccgcc
      481 ggttgttcca cgctagtgtt taccgtagat atgccgaccc ccggcgcacg ttatcgtgat
      541 atgcactccg gaatgagcgg cccatacaaa gacattcgcc gtgtgttgca ggcaatggtt
      601 cacccgtttt gggcgtggga cgtgggcatt cacggtaaac cccacacgtt aggcaatgtg
      661 tcaaactata tgggtaaacc gattgatctg aacaactaca tcggctggct gaccgataat
      721 ttcgacccat caatttcgtg gaaagattta gagtggattc gtgaattttg ggacggcccg
      781 atggtgatca aaggcattct cgacccagaa gatgctaaag atgccgtaag atttggtgcg
      841 gacggtatca tcgtctccaa ccacggtggt cgtcagttag atggggtact ttcttctgcc
      901 aaagccctgc caagcattgc cgatgcagta aaaggggaga tcaaaatcct tgccgactct
      961 ggtatccgca acggcttaga tgtggtgaga atgctggctc tcggcgcaga ttgtacgatg
     1021 atcgggcgtt cctttgttta cgctcttagc gccgccggtc aagccggtgt ggaaaacctg
     1081 ctcgacatct tccttaaaga aatgaaagtg gcgatgacac ttaccagcaa tgctaaaatt
     1141 agcgacatcg gcagagatgc tttggtagat ttaagtaatc tttagctcaa atagccacaa
     1201 tccgcacgtt ttaggtgcgg attgttattt tagataggct aatttggcat tttggtaata
     1261 ttaatgcggt aatatttcaa tgtatcttgg ttgaaaaaga gaaattcatc gttatcttta
     1321 gttttttcgc ccagcacata aatgttattg cgatgaatag ataagacata atatcgctta
     1381 ttttcaattt caagataatc atacgttgtt ctcagctcag ggcgaatgta gccaatactc
     1441 aaagaaagta agctaaaata gatgaaaata aaaatataga aaaaaccgaa gcgttcttcg
     1501 cttaacattt tttgaatatc taagtggaat acgcggtggc cccaatgttt gtgaaataat
     1561 aatactgata gggtagttgt ccctagatag aggtagttgt ccctagatag agtaggtgta
     1621 aatacattgg cactttgcca ataaacagat agaaactgat cattactggc aagctaaaaa
     1681 tagtaacaag aacactcacc cgtagccagc caaggtattt aaagtaatgc agcttaaaaa
     1741 ctttattaac taaagcgtat cctatcaaat aaaaaagaaa gatagttaaa aatgtgccaa
     1801 aaacgtaggc tagtgcgcga gctataccgg cattaccaac ctcaacatgc caccacgggt
     1861 agccatgaaa agcagcaata ccccaagcat aacagtaagc ggtagaaaaa gagattaaag
     1921 taaagaaagc gagagtaaga gaagagttga tgaatttctg attagtcata acagtaaata
     1981 gattgcacct acttagttaa agatgaaaca caagaaagca gactgagttt agcttccacc
     2041 caactccttt gtttagagga gaactttatt aaagcacgac ttttgttgta tttcaaacct
     2101 ttgtttttat acaaaacaac cgtgttgtaa aaaatgatcg taaactgacc gcttgtaatg
     2161 cactctgaat atatcgaaac atgataaaaa tgataaagca tgtttcgata atttactgtc
     2221 tttagtcttt taaatgtttt gcatgaaagg ctatatgttc accgataaag ctactaatga
     2281 aatagtagct gtgatcgtaa ccatcacgta aattgaattg gtagggtatt ccaagtctat
     2341 ctgcggtttc acagaaaatt tccggcttta actgatcgct caaaaagctg tcttggctgc
     2401 cttgatcgat tagaattggc aagcttgggc gatatttttc cagcaatgcc acgctatcat
     2461 acgccttcca agcggtctga tcttcgccta aatatgctaa aaatgctttt tgcccccaag
     2521 gaacttgggt tggggcgaca atcggtgcaa atgccgatac actttgatat tgccccggat
     2581 ttttaaatgc aatggttaag gcaccatgtc cgcccattga atgcccaaaa attgattgtt
     2641 taccgttagt cgggaaatga gcattaatca atgttggcag ttcgctgaca atgtaatcat
     2701 acatttggta atgtgttgtc cattcgcctt gcgtagcatt taggtaaaat ccggcaccct
     2761 gacctaaatc ataagcctca tcattagcga cattcacacc cctagggctg gtatccggtg
     2821 cgaccacaat cagatgatgt tcgtcggcat aacgttgaaa gccagattta gtgatgaagt
     2881 tctgctcggt acaggttaaa ccagaaagcc aatatagtac gggtagcttt tgtgttttag
     2941 cagattgcgg cagataaata gcaaatttca tttcacagtt taagctggct gagaaatgtt
     3001 gccaaacctg ttgttcaccg ctaaaacagc ggtagttttc aaattgttgc ataatattcc
     3061 ttacaagcgg tttaattccg ctgatttttt gcaaaaataa aggcggaact caccgccttt
     3121 atattgtaat atcaaccttt caaacttccc atatcaatca caaaccgata tttcacgtct
     3181 gatttcagca ttcgttcata agcatcattg atgttttgca tatcaatcat ttccacatcc
     3241 ggcacaatgt tgtgttcggc gcagaagtcg agcatttctt gagtttcctg aatgccgccg
     3301 atgacagagg cggaaataga gcggcggcct aaaatcatcg gtacggtgtt gatggtttgc
     3361 tccaactcac cgaccaagcc caccagcacc aatgtgccgt ccaacgctaa ggttgggata
     3421 taaggtttca agtcgtgggt ataaggcacg gtatcgatga ttaaatcaaa ggtatttgca
     3481 acgtctgcca tttgcttttc atcggtagaa agcaccacac ggcttgcgcc caaacggtaa
     3541 gcatcatcgg ctttacccag tgagcgagta aaaagcgtaa cttccgctcc cattgctttg
     3601 gcaagtttaa ccgccatatg ccccagtccg ccaagcccca caacagcgac ttttgagcca
     3661 gctttgacat tccaatgacg caggggcgaa taggtggtaa tgcctgcaca caaaagaggg
     3721 gcaaccgctt tggtgtccaa gttttcagag acttttaata caaaatcttc actcaccacc
     3781 acgcatgtgc tgtaaccgcc ttgtgttggt gtgccgtcaa agcggtcaat accgccataa
     3841 gtgccgatgt tgcccacttc gcagtactgc tccaaaccgt gctggcaagg actgcattca
     3901 cggcaggaat ccaccataca accgacaccg accaaatcgc cgatttggaa ttttttggcg
     3961 tttttaccga ctttgataat acgaccgatg atttcgtgcc ctggtacaag cggatagctt
     4021 tgcgtccagc cccaatcatt gcgagccata tgcaagtctg aatggcacac gccacagtac
     4081 aaaatatcaa tcaaaacatc gtcttcaagc aattcacggc gttcaaaact gtgtggggca
     4141 agtggtgtgg ttgccgaaaa agtggcgtag gatttgatag ggtaagccat ttttggggtt
     4201 taaatggctg aaatggcaat tactatagtg ccgatgattt attattgaaa atcaagattt
     4261 ttgataaatc ggtatcagtt aaaatgataa ttgggggttt tcagactgac tgaaaaatta
     4321 ttttgcccat tttaaaaaca aaaacaccgc cctcatcaca aaagcggtgt ttttcatcaa
     4381 aattgtgtca aagcatactc tgccaattct gtaagcactt catcagcagg acgctgagtg
     4441 ggttttaaat tgagtgccaa atgtttcacg cctttttctt gttcttttgc ccaaaagtct
     4501 gccaagcctt ttgcaccacc acgcagataa atgttgttaa ataactgtgc tttggcgttt
     4561 ttgtcgtcca aaacttccac aaattgagca tagccaaatg ctgtgggggt gtctttttgg
     4621 cgtaaatcgg caagggtgtt gatgatgttt gcggtgtcgg caggattcac accgtgccaa
     4681 atccacgcat caggtacatt agcaagccag tccatttctt gccttgctcg tccaatcgcc
     4741 acaatgggca agcggtgttt gggtttgggg agcaaatcca aattgtctgt taaatcggca
     4801 taatgtttgc ctttgaaatg gggaaaatcc tgctcggtta aggtgttaat caaatcaatg
     4861 ctttcacgaa aacgctcgct acgagaggca aaatccgccc caaatgcagg gtattccaca
     4921 ggtctatcgc cagaggataa gcccaataaa aaccgtccat gactcaaact atccacactt
     4981 gtcgccattt tggcaatgtg aatgggacta cgcaaagggg caatcagccc tgccgtgcca
     5041 agggcgatgt tttgggtgtg ggcggacaaa aaaccaagtg taaccatcgg gtcaagccct
     5101 tgccccacat cgccaaaatg agggtcataa aacggcacat ctcgtaccca caatgctgaa
     5161 aagcccaagt tttcagccag ccgtgcattg tcggcaatgg tggtcaaatt gtctggaatg
     5221 tgattttgat agcccataaa gggggcaatt aagcccagtg ttaattgatt ttgcccaaaa
     5281 acttgggaga aaatggggga aagcgtgttc attttggcgt acctgtctta tttaagataa
     5341 ggtattttaa gggcttttga gtttgcttaa aatgggataa attgagaaag tgttttgcta
     5401 aattggcata aatagacgct ttcaagctga ctgaaaattt gcaaaatttt taataaaaat
     5461 gaccgcttgc ctttcaacaa gcggtcattc ccctttaatt ttttaccacc gtaaacacct
     5521 tatttttcag ccgatattca cttttttcat cgtgagccag ttgcacaatg gttggcttgc
     5581 gtttacacaa ttcacgcatt gcacacgccg tcaatgttgc cagcaagcat atcttgttca
     5641 gagtaagagg catcgccgcc aatcaaaata taatgctcgc ccatatccac caccaccgcc
     5701 aaatgcccca gcgtatgtcc tgcggttggc acaaggcgga ttttgccgtc tttggtgagt
     5761 ttatgactgc tctcaaaact ttcaaacgcc ccatcattaa aatagatgcc atcaggctta
     5821 aaccaatcag gatagtgcat tgttaaataa ccgttcatca ccgcattggg ggcgttgatt
     5881 gcctgttttt cagtctcact catcacaaat cagctgtttg ggaaattggg caatccgcct
     5941 gcgtggtcgc cgtgcatatg ggtcattacc accgtgtcaa tgcgtaaagg gtcaaagccg
     6001 tttgccttta aaatttcgcc cacttcttcg tgttttttga ccccacgccg ttcgcaaaac
     6061 tgcataaacg ggtgccacca cggctgatag cccttgtcgt tggcacgact gctttcgccc
     6121 gtatccacca aaatcaaacc gtcatcgtgt tcaatcagcc agcaaccaat cggcagttta
     6181 ggcgaccatt ttttatcggt cagcacatca agcacacggg caggacgggc aaaaaagcgt
     6241 gccacttgat gatgaatttt gatttgtacc caacctgttg tcagacaatg cactttaatc
     6301 atttttgtgt tctcattaaa aaatcgtggg catattgtag aaagtgcggt aacttttcac
     6361 aaacattaaa aagttcgtat ttggctggat atgcagataa aattacaaaa atgtatgctt
     6421 attttgaaaa aagatgaacg atttaagagt aatcaaaacc catctagcaa tccaaaacgc
     6481 tttgattgaa ttattagatg aaaagctgtt tgaaaaagtg caagtccaag aaatcattga
     6541 aaaagccttg attaaccgca ccacttttta ccgttattat tcgggcgaaa gtgatttggt
     6601 cggcaaattg ataaaagagg tgaaggcgga atatcaagac ctgctcaaaa agcgttttga
     6661 aagcggtaat ttgctgcgat ttatgcaaac cattggcaac cgcttattgg aaaaaagacg
     6721 gctgattttg gcactttggc aaatcaaaac ccctcgccat catttgtatg acgatatgca
     6781 catgctgtta aaaaacgcct ttatcgccca cgccaaacgg ataacatcag atgataaaaa
     6841 ctgggctgag aaaaattggg attaccaagc ccatattttt gccaccattg cccttgaaag
     6901 ccataaatat tattttgaac aaggcaaact tttgcccatt ccacaggtgt ttgaggagtg
     6961 gatagaaatg gtgcaggtgt tgaaaagtgt ttagaaataa ctggtaaaat tttccataaa
     7021 aaacaccgct ttcatcacaa aagcggtgct ttcaaatcat caaaccttaa ccacaatctt
     7081 accaatctgc tgattgcttg ccatatattc gtgagcttta accatttcat caaaggcaaa
     7141 gattttgtca atttcagggc gtaactgacc gcttgccaaa ccgtcataga caaaggtttt
     7201 ggcttgtgcc aatttttcag gtacggtggt aatttcaaag agttcatacc cccgcacggt
     7261 caaatgctta cccaaaatcg ggaacactgg cacagcaata tcatcatggc ttaacgctcc
     7321 ataaatgatg taacgaccat cttgtgccat tgcattgatg atttttgccg cctcttttct
     7381 gcccacaggg tcaaatacca cattgacgcc tttgccattg gtgatttcaa aaagtttggc
     7441 ggttacatcg tcttcgcttg tggcaatcac aaaatccgct cctttttcca aaagtacatc
     7501 gcctttggcg tgggtgcgag acaaggcaat cacggttgcc ccttgcattt tggcgatttg
     7561 gatactggct aaaccaaccg aactggttgc accgcccagc accacaaaat cgcctttttg
     7621 tactttgcca aactcaatca gtccgccata agcggtaaca aacgccatcc aactggcgga
     7681 cgcttgttcc attgacaaat tatcagggtg cttgaccaca gcgtgttttg gcatattgac
     7741 gatttcgcca taagtgccgt attcggtaaa cataaaggac ggaataatgc tcaccttatc
     7801 gctaatggca acttcgttca caccctcgcc aatcgccaca atcacaccag ccccttcata
     7861 acccaaagtc gcagggaata cagggtcaat cacatacgcc ccttcacggt acatcatttc
     7921 ggcacggttt aagccaatgg cttggatttg gatttgtact tcgtgtgctt ttggtgcagg
     7981 aattgctaca tcaacgattt gcaagacatc aggcgaacct gtttttgtaa attggatttg
     8041 tttgctcatt gttttgacct tttgtgtaaa taatgggcgt attttaatgc aatgaattta
     8101 ttttaaacac aacatcaatt gcaaaacact tttgctaaat ttacataaat acattttcac
     8161 aatgtctgaa aagttgtttt gcaaatttta aaaagaaaac caccgcttgc cgatcacaag
     8221 cggtggtttt tgtgggttat tttgcaagag gtgtcagctt ggctacagta agcggataaa
     8281 tgcgacgccg gctaagagta aggtaacgcc gatgatctgc agcggtagaa tgggtttttg
     8341 tgtaatgccg aacagaccga atttatctac cagcaggctg ccgcaaatca ggccagagag
     8401 gacgagcatc accaccgagc ctgtgccgat ttgggggacg agccaaacgc cagcgaggat
     8461 aaacaggctg cccagtatgc cgccgctcca tatccaaaac ggtttgcctt cggttggctg
     8521 gcagagtttc ggcaggcttt tttcataaaa cagcactacc agtaataagc cgatggcacc
     8581 gacaccgaag gagactgttg ctgcgccgag tgatgagccg ggctgtctgc cgaattcgcc
     8641 attaacggcg gcttgggttg ctaatgcggt tccgcctaat atgccgactg cgcgccacag
     8701 ccataaattt gcgttttctt tgcgttttag accggacaga tggggcagta ccacggcaat
     8761 ccatacgccg aataagacta aaccaatgcc aaacaggcgg ctggccgtga aggctatgta
     8821 aggcgagtta agccaagcga aagtgtcgat cagcacgccc atcagcactt ggcctaaaat
     8881 cggcatgacc gcagtttgta cgccgccgag cttggggaag atcagtacgt ttgccgttag
     8941 ccccaccatg ccgagtaatc cgcccgtcca tgcccataag gggacgttgc ctaaattgtc
     9001 gaggctaaat gctggtgttt gttgcatgat gagcgagagt ccgagtagaa acaggaggcc
     9061 tacgctgaaa gagacaaacg aggttaaaaa tgccgcaccc acgtgcatac gcaaacgggc
     9121 gttgatggct gattgaaatg caatcagcac gccggctaaa ataccgataa taaatgtcat
     9181 gactaactgg gttgtgctgc cgcctcaaaa tgagtggtgg ttgaaaagaa atgccgtctg
     9241 aatgcggatt cagacggcct taaggcttgg tatcggcgtt tacctgatgt tgaatagccg
     9301 ttttgcctag ccggtttatt cgatgaattt caccacggtt tcgaaatctt tacgtgcttt
     9361 ttggcggatt tctttatcac ggtaaccgaa ggtacacatc accgatacgc cccattcgct
     9421 tgggtcaaat aatccttccg ctgccaaaat atcgttgact tcttggtaag cgaaaccttc
     9481 tatcggacaa gaatctacgc caattaaagc tgcaccggtc atcatattgc ccaatgcgat
     9541 gtaggtttgt ttgcttgccc agtcaaacag gctgcgttcg ctgtctaaaa tgccgatatc
     9601 tttctcttgg aattttttat acacggccat ggctttttcg cgcgcttcgc cttgtaaacc
     9661 tcgtctatcc ataatttcag aaaaatattg gctgtcgtaa cgggcatttt tcttcgctaa
     9721 aatcaccacg atatggcttg aggtttccat agttggaatg ccccagcagt aaggtttgat
     9781 tttttgccgt aattcagggt tttgcaacac taaaaactgc caaggctctg agcctaccga
     9841 acttggactt aaacgaccga gttccagaat atagttgaaa tcttcagccg gaatttttct
     9901 gctgccgtcg taagaacggg tagatgcacg gtaatgaaag gcattgatta cgtcttcttt
     9961 gttgaaaaat tgcatggaac actcctaaaa atgcaaaaca gtcttttcag acggtctgaa
    10021 aagtctagat gaaatacgga taagtataaa agatttaagt tatttttaat aattgataat
    10081 tttgatattt aggtataagt tgacgtgatg agaaaaaaca gaccctctga atgttcaggc
    10141 ggtctttaac gttggttgtt tacataaata atccgcgttc atattgcttc ggtacaaact
    10201 gcatttcact gctgtctgct gcattttggt catattgcgc attcaatacc cagtacggat
    10261 cgcgcaataa ggctcttccc acagccacta aatccgcatc gcccatacct aagatatgat
    10321 cgcctaccgc cggattatcc aacataccta ctgcaattac cggtttgccc gtagcgagct
    10381 tcacttgacg ggcaaagtct acttggtaac ctgcgtaaaa ttcggggtga ttacctgcat
    10441 gcaatacacc atcaccacca ccgctgatgt ctaagacatc tgcgcctgca tcggcaaaac
    10501 gtttcgctac ctgcaaacca taatccaagc caaagccgtt ttcaccgtat tcttgtgcag
    10561 aaatgcggac aattaacggc atttcagacg gcatcacttc acgtgctgcc tgaatcactt
    10621 gctcgccaaa caacattttg tcttggccgt actcatcact gcgctgattg gatttcggcg
    10681 aatagaattg gtgaattaga taaccgtgtg cgccgtggat ttcaattgta tcaaagcctg
    10741 cctcaacggc acgtttgacc gaatccttaa aggcttgaac caatgctttg acttcgtctg
    10801 tcgtcagctc gcgaggtgta atcagatttt gtccggcata atccaactca ccgtagtgaa
    10861 ttgcacttgg agccactgca tccggctcgt cttgggcttt gcgtccggca tgggcgattt
    10921 gcacaccgat ttttgcccct tgagcatgaa cgctgtctac cagttttttg aatgcctgtt
    10981 gttgttcatc gttccataag cctaagcagt ttggcgtaat tctgccgttt ggcgccacat
    11041 tggtcatctc cacaataatc aagccgacac cacctactgc acggctggtg taatgcacga
    11101 aatgccaatc gttcggtacg ccgttggttg ccgaatattg acacattggt ggcataacca
    11161 cacggttttt caatgctaag tttttaatgt taaacggggt gaataatttt ctgaacttcg
    11221 ccatgttgcc ttccttaatg aaatagatta ggtggaatga taattcagtg ctattatcta
    11281 ttcaaattga ttattgtgat agttaggtat aaaaatgagt gatataagaa cactggattt
    11341 caatttgctc aaagcttttg tggtgctgct ggacgaatgc aacgttagcc gggcagcaca
    11401 acgattatcc atcacccaac cggcaatgag cggtatttta aaccgcttgc gcgagagttt
    11461 taacgatccg ttatttgtgc gagtgcaaca cggtatgcag ccaaccgacc gcgcgttgca
    11521 actggggcaa accgcacgca acattctgca agatatcagc tccatgctgc aaccacccat
    11581 attggaaccg gaacatctga atatgacctt gcgtattgcc gcaatggatt atgtacaaca
    11641 aattattgcc ttgccgctga ttctgcgttt aagacggctt gctccgaatg taaaagtcgc
    11701 cttactgccg gtgcaggggc aaaatatcaa aactttgttc gagcaaaaaa aaatcgatct
    11761 tgccttggtc agccgccaac atttaagcac ggatacgctg aaaacggtat tatatgaaga
    11821 acgctacgta tgcgcgatga gcaaaacaca cccgatggcg caaacaccgc tatcgctcga
    11881 tcaattctgc aatcttccct tcgcaatgct ttcttacaac ggcggagaat tcagcggagc
    11941 tacggatcta gcgttacaaa agataggact acagcgcaaa gtaatggtct cggtaaacca
    12001 tatttccctg ctgccccagc tgctacaagg ttccgactta gtcgcagtat tgcccgaacg
    12061 cttagcaaaa accttaccca atgtttattt acaacctccg cctatagagg tagaaggctt
    12121 caccatgatg atggcttggc acgaaagaac cgaacaagat acggcgcatc gatggttaag
    12181 agaggtgctg cagggtgtgg taaaacctaa acaggctctt taattattct ttcttttgct
    12241 atgaaaaaag ccgtttatat ttttagataa acggcttttt tgcattctaa tattagtatt
    12301 tttctacaaa ctgagcatag gcattaatga agttttgcat tgagcttttt aatgtctcag
    12361 aggcaatttc accctgttca tcaaatacgc ctgtaaataa gtttcctacg tttaatgcga
    12421 aaggtaaggt tggcatattt acaaatgagc ctgaagtaat agtacgtact tgatcagctg
    12481 cacgtacacc gcctgccggg cttgcgccaa cggttacgat acctgccggt ttgccaatcc
    12541 atttgctttg ccccattggg cgagaaacca cgtcaatcgc atttttaagc attgcagaaa
    12601 agcttgcatt atgctcaggt gtgactaata acacgccatc tgccacagca accgcttcac
    12661 gcactctggt gtattgtgcc gggctgtttt catctaaatc acggtcgtaa agtggtaagt
    12721 ctgcaatatc cacaatgttt aattgaatgc ttgcaggtgc cattttttgt aagtgacgaa
    12781 cagtcaagtg actaaatgaa gttttgctgc cgctaccaat taatactgca atttgttttg
    12841 ccattttata attccttagt acatagagaa tataggcatt ttatcccttt caatctgttt
    12901 cataaagcgc ttatgatgaa aagcactatt tacttatatt aaataatttt gca
//
LOCUS       DANLUA010000062        12925 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLUA010000062 DANLUA010000000
VERSION     DANLUA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..693
                     /gene="fkpA"
                     /locus_tag="PXB44_001236"
     CDS             1..693
                     /gene="fkpA"
                     /locus_tag="PXB44_001236"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5281715.1"
                     /translation="MVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMGKNINGVIESQK
                     DIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKINEEKTKATVAA
                     GDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGTLTDGTVFDSSY
                     DRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGKIPANSTLKFEI
                     ELLDFKAADAKK"
     gene            790..1446
                     /locus_tag="PXB44_001237"
     CDS             790..1446
                     /locus_tag="PXB44_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5281716.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            1446..1823
                     /gene="tusD"
                     /locus_tag="PXB44_001238"
     CDS             1446..1823
                     /gene="tusD"
                     /locus_tag="PXB44_001238"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL5281717.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            1825..2196
                     /gene="tusC"
                     /locus_tag="PXB44_001239"
     CDS             1825..2196
                     /gene="tusC"
                     /locus_tag="PXB44_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL5281718.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            2206..2481
                     /gene="tusB"
                     /locus_tag="PXB44_001240"
     CDS             2206..2481
                     /gene="tusB"
                     /locus_tag="PXB44_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL5281719.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            2597..3496
                     /locus_tag="PXB44_001241"
     CDS             2597..3496
                     /locus_tag="PXB44_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281720.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            3846..7871
                     /gene="rpoB"
                     /locus_tag="PXB44_001242"
     CDS             3846..7871
                     /gene="rpoB"
                     /locus_tag="PXB44_001242"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL5281721.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            8134..12417
                     /gene="rpoC"
                     /locus_tag="PXB44_001243"
     CDS             8134..12417
                     /gene="rpoC"
                     /locus_tag="PXB44_001243"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL5281722.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         3916 a         2479 c         2949 g         3581 t
ORIGIN      
        1 attgttggca cactttttag tacaaatgtg atggcagaag aaaaagcaga tcctaaattt
       61 gtggatgatt cctcttatgc cgtgggtgta ttaatgggta aaaatattaa tggtgtgatt
      121 gagtcacaaa aggatatttt tacctataac caggataaaa tcttagccgg tgttcaggac
      181 accttaaaga aaaccggtaa attaaccgat gaagatttgc aaaagcagtt aaaatcgtta
      241 gatgcctatt tatctgcgaa agaagcaaag attaacgaag aaaaaaccaa agcaacggta
      301 gctgcaggcg ataaattccg tgctgattat aaaaaacagt ctggcgtaaa agaaacggct
      361 tcaggcctgt tatataaaat tgaaaaagca ggtgaaggca attcaccaaa agcggaagat
      421 actgtgaagg tgcattacaa aggcactttg actgatggca cggtatttga cagctcttac
      481 gaccgtggtg agccgattga atttcagtta aatcaattaa ttccgggttg gattgaagcg
      541 attcctatgt tgaaaaaagg cggtaagatg gaaattgtag tgccacctgc attagcttat
      601 ggtgatcgtg ccgcaggcaa aattccagca aattcaacct tgaaatttga aattgaatta
      661 ttagatttta aagcagcaga tgctaagaaa taattcgtta ttccttaatt attgaagata
      721 cgtaagcggt tagatttatt ataatatttg caaaaaatgt gataaaacta accgcttgta
      781 atttgtatta tgaccaaaaa atttacgcct ttttctgacg aagatcacgc cattcttgct
      841 tcttactttc cgatggtaga tgggattgcg gcactgattg gtgagcattg cgaaattgta
      901 ttgcattcgt tagagtttct ggaacattcg gcaatttata ttgctaacgg acacaatact
      961 aaccgtaaaa tcggctctcc gattactgac cgtgcattga aatcgctgca tcatatggca
     1021 acggatagcg tctccaaacc ttattttacc aaggcaaaag gtggcgtttt aatgaagtct
     1081 atcactattg cgattcgtaa tggtaaacag cacgttatcg ggttactgtg cattaatatt
     1141 aatttggaag tgcctacctc acagtttctc aattctttta ttgcccctgt agataccgct
     1201 tcagaagtta cttttgccag ttcagtagaa gatttggttt cacaaacgat tcaaacgacc
     1261 attgatgatg tgatggcaga tcgtcaaatt tctaacaata ataaaaatcg tcaaattgtg
     1321 atttcgttat ttgagaaagg gatttttgat attaaagatt ccattaacca agtagcggag
     1381 aagctggata tttcacgcca tactgtgtat ctctatattc gtcaaattaa gcaggacaat
     1441 gattaatgga ttatgtatta gcgataaaat caccggttta tggttcacaa ggggcatttt
     1501 tagcctacca agtggcggaa gaattattaa aagccaatca tacaatttgc caaatattct
     1561 tttttcaaga aggcgtgagc aatgctaatg ccttagtgaa tccagcaagc gatgaagtga
     1621 atttagtcga gatgtggaaa aaattggcta aattgcaccg cttgcctctg catttgtgca
     1681 ttgctgctgc ccagcgtaga ggcgtggtgg atgagcaaat ctcagctaat gcaacacata
     1741 atttagccga aggatttacc ctagccgggt taggcgaatt tagccaagca gtattaaaag
     1801 cggatcgatt actgactttt taagatgaag aaatataaac ttgccttttt atttacccag
     1861 ccaccttttg gttcggcagt tagccgtgag gggctagatg ccttgcttgc agctagtgct
     1921 ttttgtgacg agcaagagat agctatcctc tttatcaatg atggcgtatt taatctcatt
     1981 gccgatcaac agccggaaaa actcttacaa aaagatcata tttccacctt caaattattg
     2041 gagttgtatg acttaactga atgttttatt tgccaagaaa gtgtaagttt acgagggctg
     2101 gataatgcag attggttact gcgaatgctt caatttaaaa ctcacgctga gatgatcgca
     2161 ttgctcaatc aagctgaaaa agtaatgacg ttttaaggaa tatttatgct ttataccctt
     2221 tccaaagcga attatgatga agaaacctta aacttctttt tctcacatct tagtgagaac
     2281 gatgcggttg ttctgtggca agatggcgtg ctacaagcgg tcaaaagtcc gcaatttttt
     2341 gcaaaattgc cccgttgcta tttattggca caagatgtgc aagctcgagg attgcaagag
     2401 aagctcactg catttcagtg catttcatta tccgaattag tcaaattaac agcggtttat
     2461 tttccccagg ttgcactgta acctagagag gatatttttt gtaataccaa ataatttaga
     2521 ttatgatgtg attcctaaaa aggagaatat tatgaaaaca aaatgtctta ttccgctact
     2581 agcacttaca cctgttatgg tgatggcaga agaaaataac acttgggtag acaagaaaca
     2641 tgaaacagta agcactacct tagatcgttg ggcaaacaat attaacagtt ggctaggtga
     2701 tgcagaccca gataaaccgg gaacggcttc tttacgggta atgttagata gccgttggga
     2761 taaacacgat aaatttactt ataagccccg tgttcgtggt aaaatcaaac tccctatctt
     2821 gaaaaagcat ctaaatgtcg tatttggtga tgacgaactg gataatgagc ttaccgataa
     2881 aaatcatgct ggaaaattgt ataaaaacgt caatccaaat aaacgctata actctcgtga
     2941 agcgagagat tcgaactctt ctatcggttt acgttggtct gatggcatta aggctttagg
     3001 gattgagacc gatttagatg ctggtattcg ttcgggaagc gatattttcg ggcgtttacg
     3061 catcagtaaa gattggaaaa tcaccgacca gttcagtacc cgcctagagc aaatttatcg
     3121 ttacggttca gagagcaagc actatttacg tactaacttt gaaaataaat ataccgaaac
     3181 ggataccacc tttattatga accacaccaa ttttgaatat cgtcatgatg tagatgaaga
     3241 aagaagttgg gggaatagtt tatatcgtca acataacttc aaaccactca cgcacatcag
     3301 ctatggtatc agtatggggg ggcgtttaga taaagagtta tcaaaattta actattgggg
     3361 tccatttgtc tcctatcgtc aaccaatctg gcgcaactgg ctctttatcc agcctgaaat
     3421 cagtttttat aacgacaaag ataaagatcg taagcacttt attcagacat ttgtacgttt
     3481 agaggctatc ttctagctat taagcctcaa gtagaatctt ggggcttgtt tttgcaagcg
     3541 gtcaatttct tgccaaaatt tacaaaatcc gctataattc ctaaccattt ttagcaaaaa
     3601 ttggctgacg gcttgaccgg tcagcctttt gtgcttgaac gcaagggctg attttgtcat
     3661 tttaatcagc aaaatttgca aatttcctgc ccaatttgac cgcttgcagt agcgatttct
     3721 gactgcttga ttggcgattg gaaaatgaca tctcgtacag tttttataat gccacttcca
     3781 accgagtctt tcgtgtcgga ttgtgtaatc tcaaccaaaa tatatttcag aggctatctt
     3841 aaaaaatggc atattcatat tccgagaaaa agcgtatccg taagagcttt ggcaaacgcc
     3901 cacaagttct gaacgtacct tatctattaa caattcagct tgattctttt gaaaaattta
     3961 tcgaaaaaga tcctgaaggg cagcaaggtt tagaagcggc attccgttct gttttcccga
     4021 ttgtgagcaa caatggtgca acagaattac aatacgtttc ttacgaatta ggcgagccgg
     4081 tgtttgacgt gcgtgagtgc caaattcgtg gtacaaccta cgctgcacca ttacgcgtga
     4141 aattgcgttt agtctcttac gatcgtgaag ccgcagccgg tacggtaaaa gacattaaag
     4201 agcaaaacgt gtatatgggc gaaatcccat taatgaccga taacggtact ttcgtgatta
     4261 acggcaccga gcgtgttatc gtttctcaat tacaccgtag cccaggtgtg ttcttcgatt
     4321 ctgacaaagg taaaacccac tcttcgggta aagtgcttta taacgcacgc attatccctt
     4381 accgtggttc ttggttagat ttcgagtttg atccgaaaga taacttattt gcccgtatcg
     4441 accgccgtcg taaattgccg gcaaccatta ttctacgtgc attaggctac accaccgaag
     4501 aaatcttaaa tttattcttc gagaaaatta aatttgaaat tgccgataat aaattattga
     4561 tgacgttagt gcctgagcgt ttaagaggcg agactgcatc atttgatatt gcagcaaacg
     4621 gcaaagtata tgttgaaatg ggtcgccgta ttaccgctcg tcatatccgt gcgttagaaa
     4681 aagacaatgt gacccaaatt gaagtgccgg tggaatatat tgtcggcaaa gtagcggctc
     4741 aagattacgt tgatttatct tcaggtgaag tgatttgccc ggcgaatatg gaaatctcga
     4801 tggagatgtt ggcgaattta tcacaagccg gttacaacca gatcgaagta ttatttacca
     4861 acgatttaga ccacggtcca tatatttctg aaaccttacg tgttgatcca acttacgacc
     4921 gtttaagtgc gttggttgaa atctatcgta tgatgcgtcc gggcgaacca ccaacgaaag
     4981 aagcggcaga agcattattc gataatatgt tcttctcggc agagcgttat gatttatcgg
     5041 cagtaggtcg tatgaaattc aatcgctcgt tagaaattcc ggaaggcgta ggcagcggta
     5101 ttttaagcaa cgatgacatc atcggtgtga tgaaaaaatt aatcgaaatc cgtaacggtc
     5161 gtggtgaagt ggatgatatc gaccacttag gtaaccgtcg tatccgcagc gttggtgaaa
     5221 tggcagaaaa ccaattccgc attggtttgg tgcgtgtaga acgtgcggtg cgtgagcgtt
     5281 tatctttagg cgatttagat ggtgtaacac cacaagattt aatcaacgct aaaccgattt
     5341 cagcagcagt gaaagagttc tttggttcat cacaactttc gcaatttatg gaccaaaaca
     5401 acccgctttc agaggtaaca cataaacgcc gtatctcggc attaggcccg ggtggtttaa
     5461 cccgtgagcg tgccggcttt gaagtgcgtg acgtacaccc gactcactat ggtcgtttat
     5521 gtccaatcga aactcctgaa ggcccgaata tcggtttgat caactcacta tcggtgtatg
     5581 ctcgtactaa taactacggt ttcttagaga ctccattccg taaagtggtg aatggtcaag
     5641 taaccgaaga gatcgagtat ttatctgcga ttgaagaaag caacaatatc attgcacagg
     5701 cgaactcaaa cttagatgag aacttccgtt ttaccgatac ttatgtaact tgccgtcagc
     5761 aaggcgaatc gggcttattc aaaccggaag aagttcaata tatggacatc tcaacccaac
     5821 aagtggtttc tgtggctgcg gcgttaattc cgttccttga gcacgacgat gcgaaccgtg
     5881 cgttaatggg tgcgaacatg caacgtcagg cagttccaac cttgcgtgcg gacaaaccat
     5941 tagtcggtac cggtatggaa aaaccaattg ctcttgactc aggtgtagcg attgtcgcaa
     6001 aacgtggtgg tacggtgcag tatgttgatg cgtcacgcat tgtagtgaaa gtaaatgaag
     6061 atgaaaccat tccgggcgaa gcgggtatcg atatttataa cttaatcaaa tacacccgtt
     6121 caaaccaaaa cacctgtatc aaccaagtac cttgtgtgaa cttgggtgag ccggttgctc
     6181 gtggtgaaat cttagcggac ggtccttcaa cagacttagg tgaattggca ttaggtcaaa
     6241 atatccgtgt ggcatttatg ccttggaacg gttataactt cgaagactca atgttagttt
     6301 ctgagcgtgt ggtacaagaa gatcgcttta ccactatcca cattcaagaa ttatcttgtg
     6361 tggcgcgtga taccaaatta ggtgcagaag agatcaccgc tgatattcca aacgtgggtg
     6421 aatctgcatt aagcaaatta gatgaatcag gtatcgttta catcggtgca gaagtgaaag
     6481 gtggcgacat cttagtaggt aaagtaactc ctaaaggtga aacccaatta acaccggaag
     6541 aaaaactatt gcgtgcgatc ttcggtgaaa aagcctctga tgtgaaagat tcatcattgc
     6601 gtgttccaaa cggtacttca ggtacggtta tcgatgttca agtatttacc cgtgatggcg
     6661 tagagaaaga taaacgtgcg aaagaaatcg aagagatgca actgtgtgaa gcgaagaaag
     6721 acttagcgga agagttggaa atcttagaag ccggtttatt tactcgtgtt cgcaacctat
     6781 taattgaagg cggcgtaaaa gaatctgcat tagatggcgt tgctcgtgaa aaatggttag
     6841 aacaaaccct tgaagacgaa gcgaagcaaa atcagttaga acagttagcg gaacagcacg
     6901 aagagttacg caaagaattt gaacgtaaac ttgagatcaa acgcaacaaa attatccaag
     6961 gcgacgattt agcaccgggt gtgttaaaag tagtgaaagt ttaccttgcg gtgaaacgcc
     7021 aaatccagcc gggtgataag atggcgggtc gtcacggtaa caaaggggtt atctcgaaaa
     7081 tcaaccctgt ggaagatatg ccatacgatg aaaacggtca gccggttgaa atcgtattga
     7141 acccgctggg cgtaccgtct cgtatgaaca tcggtcagat cttagaaacc cacttaggct
     7201 tagcggctca tggtatcggt gatcagattg ataagatgat caaacagcaa caagaagtgg
     7261 ctaaattacg tgaatatatg caaaaagcct acgatttagg tcacggctca caagtcgttg
     7321 atttaagcac tttctctgat gaagaagtga tgcgtttagc gcaaaactta cgcaaaggct
     7381 taccgcttgc tactccggta ttcgatggtg cacacgaaag cgaaatcaaa ggcttattag
     7441 agttaggcgg cttaccgact tcaggtcaga tcacacttta tgatggtcgt accggtgaga
     7501 aatttgagcg tccggtaacc gttggttata tgtatatgct caaattgaac cacttggttg
     7561 atgataaaat gcacgctcgt tcaaccggtt cttatagcct tgttactcaa caaccattgg
     7621 gtggtaaagc tcaattcggt ggtcagcgtt tcggtgagat ggaggtttgg gcattagaag
     7681 catacggtgc ggcttatact cttcaagaaa tgcttacggt gaaatcggac gatgtgaacg
     7741 gtcgtacgaa gatgtataaa aacattgtag atggtacaca ttatatggag ccgggtatcc
     7801 ctgaatcctt taacgtaatt accaaagaga tccgtgcatt agcaattgat atggagctgg
     7861 acgaagcgta atggtttcac tctccccctt gatgggggag agacaggcaa aggatttgtt
     7921 cagaatcctt tggcagagag agggtgatgc aagcggtgta aatttgctaa atttttgcaa
     7981 aaatcgaccg cttaccctct ccctgatttt gcttctgaac gaggcaaaat ctatccctct
     8041 cccacaaggg gagagggttt aaaatcgcaa ccaaattcgt agagtggata ttctttccac
     8101 caaacaaaac aacctcaaac taggggcaaa aaagtgaaag acttagtgaa gtttttaaaa
     8161 gcacaaacaa aatcgaacga agattttgat gtgattaaaa ttggtttagc atcaccggac
     8221 aagatccgtt cttggtcttt tggtgaagtg aaaaaaccgg aaaccattaa ctatcgtacc
     8281 ttcaaaccgg agcgtgatgg tttattctgt gcacgtattt tcgggccggt aaaagattac
     8341 gagtgcttgt gtgggaaata caaacgttta aaacaccgtg gcgtaatttg tgaaaaatgt
     8401 ggtgttgaag taacccaaac taaagtacgt cgtgagcgta tggggcacat tgaattagct
     8461 tgccctgtcg cacacatctg gttcttaaaa tcactaccgt cccgtatcgg tttaatctta
     8521 gatatgccgt tgcgtgatat tgaacgtgtg ctttattttg aatcttatgt agttactgaa
     8581 ccgggaatga ccgatttaga gaaaaatcag ttattaaccg aagaacaatt tatggacgct
     8641 gaagagcgtt ggggtgatga atttgaagcg aaaatgggtg cagaaggtat ccaagcatta
     8701 cttcgtgatt tagatttaga acaccaatgc gaaaccttgc gtgaagagtt acaagaaacc
     8761 aattcagaaa ccaagcgtaa aaaaatcact aagcgtttaa aaaccttaga agcattctta
     8821 caatccggca acaaaccgga atggatggtg atgacagtat taccggtact tccaccggat
     8881 ttacgtccgt tagtaccgct tgacggtgga cgttttgcga cttctgactt aaacgattta
     8941 taccgccgtg taattaaccg taacaaccgt ttaaaacgct tgttagattt agttgcaccg
     9001 gacatcatcg tgcgtaacga aaaacgtatg ttacaagagt cggttgatgc gttattagac
     9061 aacggtcgcc gtggtcgtgc gattaccggt tcgaacaaac gtccgttaaa atctttggca
     9121 gatatgatca aaggtaaaca aggtcgtttc cgtcaaaact tattaggtaa acgtgtagat
     9181 tactcaggtc gttcggtaat taccgtaggc ccatacttac gcttacacca atgtggttta
     9241 ccgaagaaaa tggcgttgga attattccgt ccgtttatct actcaaaatt agaaaaccgt
     9301 ggcattgctt caactattaa agccgcgaag aaaatggtag agcgtgaaga tcctatcgta
     9361 tgggatattc ttgccgaagt gattcgtgaa cacccgattc tacttaaccg tgcaccaaca
     9421 cttcaccgtt taggtatcca agcgtttgag ccgttattga ttgaaggtaa agcgatccag
     9481 ttacacccac tcgtgtgtgc ggcgttcaat gcggacttcg acggtgacca aatggcggtt
     9541 cacgttccat taacccttga agcacagtta gaagctcgtg cgttaatgat gtcaaccaac
     9601 aacgtacttt caccggcaag tggtgatccg attatcgtac cgtctcagga cgttgtactc
     9661 ggtctttact atatgacccg tgagaaagta aacggtaaag gtgagggaat gtacttcctt
     9721 gaccctcgtg aagcggaaaa agcataccgt accggtcagg ctgaattaca ttctcgtgtt
     9781 aaagtgcgtg ttactgaata tgagaaaaac gaagcaggcg aattagaagc aaaaacttcg
     9841 ttagtagata ccaccattgg tcgttcaatc ttatggatga ttgcaccaaa aggtatgccg
     9901 tttagcttgt tcaaccaaac cttaggcaaa aaagcgatct cgaaattaat taacgagagc
     9961 tatcgccgtt taggtttaaa agaatctgtg gttttagctg accaaattat gtacaccggc
    10021 tttgcttatg ctgctcgttc tggtgcctca gtaggtatcg acgatatggt aattccggag
    10081 caaaaatacg caatcattga agcggctgaa gcggaagtcg ctgagatcca agagcaattc
    10141 aacggcggtt tagtaacagc cggtgagcgt tataacaaag tgatcgatat ttgggctgct
    10201 gcaaacgaac gtgttgcgaa agcaatgatg gaaaaccttt caacggaaga agtaatcaac
    10261 cgtgaaggta atccggaaaa acaagcctca tttaacagta tctttatgat ggcggactcc
    10321 ggtgctcgtg gttctgctgc acagattcgt cagttagcgg gtatgcgtgg cttgatggct
    10381 cgtccggacg gttcgattat cgaaacccca attactgcaa acttccgtga aggtttgaac
    10441 gtattacagt actttatttc aacccacggt gcacgtaaag gtcttgccga tacggcatta
    10501 aaaacagcga actccggtta cttaacccgt cgtttagttg atgtggcaca agatttagtt
    10561 atcattgaag atgactgtgg tacgcacgaa ggtatcgtga tgacaccact tatcgaaggt
    10621 ggtgatgtga aagaagccct gcgtgatcgt gtattaggtc gtgtggtggc agaagatgtg
    10681 ttaaaaccgg gtacagatga agtattaatt ccacgcaaca ccttattaga tgaaaaatgg
    10741 tgtgatgtgg tggatcgtga atctgtggat gtgatcaaag tacgttcggt agtaacttgt
    10801 gatacagact tcggtgtgtg tgctaaatgt tacggacgtg acttggctcg tggtcactta
    10861 atcaaccaag gtgaagcggt cggtgttatt gcggcacaat ctatcggtga gccgggtaca
    10921 cagttaacga tgcgtacgtt ccatatcggt ggtgcggctt ctgcggcagc aaaagaatct
    10981 agcatccaag tgaaaaacgc aggtacgatt aaattaacca acgctaaatt tgtaaccaac
    11041 aaagaaggca aaatcgtatt aacttctcgt aatactgaat taactgtcat cgacaccttc
    11101 ggtcgtacca aagaaaacta taaagtgcct tatggtgcgg tgctttctaa acacgacggt
    11161 gcagaagtgg ctgtgggtga agtggttgcg aactgggatc cgcacacaat gccggtaatc
    11221 tcagaggtat caggtcgtat ccaatttagc gacatcgtag atggcttaac cgttactcgc
    11281 caaaccgatg aattaaccgg tttatcatct atcgtggtgc aagatgtggg tgaacgtgca
    11341 accgcaggta aagatttacg tccggcatta cgcttagttg atagcaacgg caaagacatc
    11401 ttaattccgg gtacagacgt tgcagcacaa tacttcttac cgggtaaagc aatcgtaacc
    11461 ttagacgatg gtgcagaaat tgaagtcggt gaagccttag cccgtattcc acaagaatct
    11521 gtggggacga aagatattac cggtggtctt ccacgcgtag cagacttatt cgaagcccgt
    11581 aaaccgaaag agccggcaat cttggcggaa atttcaggta ttgtgtcgtt cggtaaagaa
    11641 accaaaggta aacgccgctt ggtaattact ccggcggaag gcgaagcgtt tgaagagatg
    11701 attccaaaat ggcgtcagct caacgtattt gaaggcgaga tggtacaacg tggtgatgtg
    11761 atatcagacg gtgcagaaac tccgcacgac atcttacgct tacgtggcgt tcacgccgtt
    11821 accgactaca tcgtaaacga agtgcaagaa gtttaccgct tacaaggggt aaaaattaac
    11881 gacaagcaca tcgaagtgat tgttcgccaa atgttgcgta aagctgtgat caccaacgct
    11941 tacgactctg aattcttaga gggcgaacaa gtggaagtgg ctcgtgtgaa aattgccaac
    12001 cgcaaacgtg ctgaagaagg caaaccgttg gtagaatttg aacgtgaatt gcttggtatt
    12061 accaaagcat ctcttgcaac agaaagcttt atttctgcag cctcgttcca agaaaccact
    12121 cgagtgctta ccgaagccgc agtggcaggt aaacaagatg aattgcgtgg cttaaaagag
    12181 aacgtaatcg ttggtcgctt aatcccggca ggtaccggtt ttgcttacca ccaagcacga
    12241 gctaaaaaac gtgcccaact ggaagcagga attgcagttg tccctgaatt tgaagcaccg
    12301 gcagctaaag ccaattcatt cgttaccgat gcagacatcg aagctgaatt tgaatttgtt
    12361 gcagacgatg caacccaaag tttagcagcg ttgttaaatg cgggtgagga agagtaatag
    12421 ctcgtagggt atgataaatt aaaaaatctc ctagaatata atttctagga ggttttctac
    12481 ttgaatataa gggaaaaata tatagaatgt aagggcttat tcaatttgtg gataaggtaa
    12541 tcaacagtat gtcaatagtt gataacattt cataaagcgt ttcagctact gaacataaac
    12601 taataaagaa tttgataatg attattgtca tattagattc cttgtgagtt ataatagttg
    12661 attgattatt ataatatata tttagcagat agcggtttat tcgacgggta tcctaaggta
    12721 cctaggctat ataaagactg ttaactattc aaatagttag ggacaaccgc aatccttagt
    12781 ctttagtttt gctttcatta aattttctat tatggatata tcttggtata agcctcgtaa
    12841 ttacttacat ttcgattcac ccataactct tcaacaagca attaaattag tcgctaatcc
    12901 aaataatgta gtaaaacata gtttc
//
LOCUS       DANLUA010000063        12888 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLUA010000063 DANLUA010000000
VERSION     DANLUA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12888
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(70..2982)
                     /gene="rapA"
                     /locus_tag="PXB44_001244"
     CDS             complement(70..2982)
                     /gene="rapA"
                     /locus_tag="PXB44_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL5281723.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            complement(3143..3442)
                     /locus_tag="PXB44_001245"
     CDS             complement(3143..3442)
                     /locus_tag="PXB44_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281724.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            complement(3563..4888)
                     /locus_tag="PXB44_001246"
     CDS             complement(3563..4888)
                     /locus_tag="PXB44_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL5281725.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            4973..6292
                     /gene="rlmD"
                     /locus_tag="PXB44_001247"
     CDS             4973..6292
                     /gene="rlmD"
                     /locus_tag="PXB44_001247"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL5281726.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            complement(6432..7613)
                     /locus_tag="PXB44_001248"
     CDS             complement(6432..7613)
                     /locus_tag="PXB44_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL5281727.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(7603..8304)
                     /gene="rsuA"
                     /locus_tag="PXB44_001249"
     CDS             complement(7603..8304)
                     /gene="rsuA"
                     /locus_tag="PXB44_001249"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL5281728.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            complement(8549..9520)
                     /gene="menC"
                     /locus_tag="PXB44_001250"
     CDS             complement(8549..9520)
                     /gene="menC"
                     /locus_tag="PXB44_001250"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL5281729.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            9732..11810
                     /gene="metG"
                     /locus_tag="PXB44_001251"
     CDS             9732..11810
                     /gene="metG"
                     /locus_tag="PXB44_001251"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL5281730.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            11820..12236
                     /gene="ndk"
                     /locus_tag="PXB44_001252"
     CDS             11820..12236
                     /gene="ndk"
                     /locus_tag="PXB44_001252"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL5281731.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(12292..12768)
                     /gene="greA"
                     /locus_tag="PXB44_001253"
     CDS             complement(12292..12768)
                     /gene="greA"
                     /locus_tag="PXB44_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL5281732.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
BASE COUNT         3699 a         3034 c         2556 g         3599 t
ORIGIN      
        1 atgacacaaa atagaaaagt gattttttcg acacagatcg aaaaaatcac ttttcgtact
       61 tgcaaaaaat tacgctttat tacttacaat cactcgcaag ctatctaagc gataatttgc
      121 cgtttcaagt tgggcaaggg aagcgttaag ctgttgttct aaggcttcaa cttcatcggc
      181 tcgaatattt ttattcacca ttttgagtga ttccaagcgg tgtaattctg cttttaattt
      241 gctatttgcc tcaaatttgg cagtttcaat cacctgttgg acctgaggtt ctaccgcttt
      301 ttcactcgct ttaattaatt tttcaatatc cgctcgagcc attttggcaa ttttatttgc
      361 catctgttta ttcaccggtt ttaattggcg ttcaagcccg gcaaaagaga cctgcggagc
      421 aatattgttg cctttgttat ccactaaaac ccgaatcggg gttggcggta ggaaacgggt
      481 taaatttagc cctttcggtg cttgtgcttc caccacataa agcgcctcaa gtaataatgt
      541 accggcaggc aatttattat tcaccagcaa tgagattgct gatttgccaa tatcgccgga
      601 ggtgattaaa tcaataccat tgcggatcat cgggtgatcc caagtgagaa attccacctc
      661 ttcccgcatt aacgccagtt cacgattaaa ggtaatggtc gtgccatctt ccgatagccc
      721 cgggaaatca ggcacaagca tatgtccggt tgggctaatc acaatgctct gctcgcctaa
      781 atcttcctgt tcaacgccaa tcacatcaaa caaactcaag gcaaaattaa ccaactgcgg
      841 attattatct tctttcgcaa tatcattagc gagctgttga gctgcttctc cgccattaga
      901 attcagttct aataatctgt cctgtccttt ctccagttcg gctttcaaat actgttggcg
      961 tttataggtt ttcgcaacaa aatcatcaaa ttgggctgaa agttcaggtt tttgcaggaa
     1021 ttgttgcaat tcttcaccta attcacggaa gatcgctgtt cccatcgggc aggtttcttc
     1081 aaacgcattc aagcccttgt gataccactc tgccaacatc tgttgagccg ataaattaaa
     1141 gcaaggcaca taaatctgaa tgtcattttt ctgtccgata cgatctaatc gcccaatact
     1201 ttgctccaat aaatccgggt tatccggcaa attaaacagc actaaatctt tagcaaattg
     1261 gaaatttcgc ccttctgaac cgatgcttga actaatcagc acctgagcgc cttcttcttg
     1321 ttgagcaaaa taggcggcag ctttgtcacg ttcaacaatc gacattttct cgtggaaaac
     1381 ggcagaacga atcgcctcac gctcacgcaa aacttgctcc aaagcaatcg cagtgtccgc
     1441 ctgtttgcag atcaccagca ctttttcatc acgatgattt tttaagaaag tgatgagcca
     1501 atcaatccga gggtcgaatt cagcccattt cgcattcggg ttcattcgtt ggaacaaata
     1561 ttcaggatag agcaaatcac tttggctcac actccccatc attcccatca cttttaacgc
     1621 attttggtat tgtttcggca tttctaggct gatttggtgg tattcccgat gtgggaagcc
     1681 tttcactccc tgacgggtat ttctaaacaa cacccggctg gttccatggc ggtcaattag
     1741 ctcactaatc agctcttgac gggcttgcaa gcggtcattt tcgtccgatt ttttgctatt
     1801 aattgcacgg aacatcggct cggtatcttg ctcgcttaac agctccgcaa tcgcattctg
     1861 ctcgtcatta cttagcggtt tatcgcttaa taagctactc accgcatctg ccaccggttt
     1921 atactgtgct tgctcctgta caaactcggc ataatcataa aaacgatccg aatctaacaa
     1981 cgccaagcgg gcaaaatggc tctcttgccc taattgctcc ggtgttgccg ttaaaagcaa
     2041 gacggcagga atttgttgag ataaacgctc taccaactga tagccgatag aagattcggt
     2101 ttccgaccat tctaaatggt gagcttcatc aacaattaat agatcccagt cggcagcaag
     2161 cacttgattg gctcgttgcg gtgaggtttc cagccagttt agtgaagtaa tgactaacgc
     2221 ttcgctctca aatggatttt cacttacatc atcgccattt tcatcttgtt tatcaaaatc
     2281 actacaacgt tcttcatcga ataacgagaa tttcagatta aaacggcgaa gcatttccac
     2341 caaccattgg tgttgcaaac tttccggcac taataccaat actcgttctg ctctgcccga
     2401 aaatagctgt tgttgcaaaa tcataccagc ttcaatagtt ttacccagtc ccacttcatc
     2461 ggctaatagc acacgaggag caaggcgttg cccgacttct tttgcaatat gtaattgatg
     2521 tggaatcagg ctggctcgaa tgcctcgcaa accacgcagt ggcgactgat attgcgcttg
     2581 ctggtgctgc aaagcgttaa agcgtaatgc aaagcgatct gagcgatcaa tctgagccga
     2641 aaaaaggcgg tcttgcggtt tgctgaaact gattttatga tcgagcaaca tttcaggcag
     2701 taccgcttct tcaccgttat ccaccctttt gcccaaatag attacggcac cttgattttc
     2761 cacaacctct gaaacctcta actgccagcc atctatactg ttaattctat ctccggcttg
     2821 aaactgcact cgagttaaag gtgcaactgc aattgcatag acccgctctt cttccgctgc
     2881 cgggaaattg atagtgacag tgcgatgatc caccgaagtc acgattccca aacctaaatt
     2941 attttccgat tcacttagcc aacgctgacc gactaaaaac attttttcct ctaagtaata
     3001 agtaaaagca tcattattgt catttaaacg gctattttac caaattccgg gaccggtcgg
     3061 cttgagtatt tttgcaaaaa agcacaataa aaagaccgct tgtgcggtta aatcacacaa
     3121 gcggtcgttt taaggggtaa atttagtttt tatctaaatc tagatctgcc ttatattcaa
     3181 tccgatttct accttttact tcaatttcaa tatccagctc ttggtcacca cgtttgaatt
     3241 ttaaatcagc atcatctcta tgaatatcag attctactaa atggaaacct ttacgtttag
     3301 cgtgggcgat cgcacttttc gctaagccac gaacatcatt accactgaca cgatattcta
     3361 cttcaaactc accattacct tgacgatctg ctttaactaa ttcgccacga ggctgataaa
     3421 tttcacttgg aagaaaccgc aaatgctgtt gcagaagcca aagttgctaa tactaaaaga
     3481 cctttagaaa cagttttcat tgaaatctcc tattaaataa atgaaaccta ttactgagac
     3541 cttgcaaaat taacaaagtt cattataaac tttcaaaaag tttaaacgcc tgcccatcag
     3601 acataccggc taaatagtcg caaattaccc ttgcacgctt agattcttcc gcatttttcc
     3661 aacgttcccg cacattatta gggagtaatc gcatcggatc actgcttaaa atatcgaaca
     3721 attccattag aatgcgttgc cccttgcgtt ccacccgctg ggttttggca tcacaaatca
     3781 catattgata cacaaaatct ttcaagattt tcagcacaca ggcaacactt tccggtaaat
     3841 aggcattata acgtagcaac ggctcgtcaa attcaggcag agctttccaa cgcacattgg
     3901 taataaaatg attcactaat gccccgatca catcttttcg ctcgtagcgg tgctgagaaa
     3961 agagcttttc ccgaatctcc ggcaggcgtt gtttcaccca atcggattgg cactccgcca
     4021 gcaatttttc cgcattttgc cacgattgcg gtgtgaccat tccacctaca atcgcatctt
     4081 ccaaatcgtg tacgccatag gcaatatcgt ccgctagttc cattatgctg caatcgagag
     4141 atttatattg cgttttgttt ggctgtttcg gatcttgatt gggctgaacc gtgcaaaatt
     4201 ggcgtcggtc tttttcactt aacggggcta atacccactc aaaaaagtgg ttgttgtcat
     4261 caaaaatccc tttgctctgt ggaaaatggt gcaaattaat atgccgagcc tcgctcattt
     4321 ctgccggctc aatcggcgag gtttgagaca agagtgccgg atatttcatt acccccagta
     4381 aggtgcgacg ggtcagattc attccggctg tttcggtgta aggctcaagc tgggtcataa
     4441 tccgaaagga ttgagcatta ccctcgaagc cgccaaaggc gtacatttta taatttaacg
     4501 ccatttcacc accgtgcccg aacggagggt gcccgagatc gtgggcaaaa caaagggatt
     4561 ccaccaagct atgatccggc aataaagcgg tcagatttgc catatttttt gcaaattcct
     4621 gctccgaaca gaccgcttga aagccttcca catccgctaa taatttttct cgcaaactgc
     4681 taccgatttg agccacttcc aacgaatggg ttaaacgggt tcgataaaaa tcatcttccc
     4741 ccacggcgtg aatttgcgtt ttcgcctgca aacagcgaaa agcctccgaa tgcaataacc
     4801 ttgcccgatc ccgctgaaaa ggcgagcggt gatcttgttc ccgtttttta tccgccaaat
     4861 aacgttcatt ccaaaccgat gaaatcatat tgcctccatt ttccgccaac tgcttttctt
     4921 atataatctc cccatttcga cactttatat cataacaatt catctgaaaa caatggctat
     4981 tttttattct gaaaaatcgg caaaaaaacc gataaaatca cccgcttgtc aaaaggcaac
     5041 tattcaatcg ttagattatc aaggcttagg gattgccaaa atcaacggca aaacttggtt
     5101 tatcgaaaat gccctgcctg atgagcaggt ggaatttaaa gtgttggaag agaaacgcca
     5161 atacggacgt ggacaagcgg ttaaaattct taaaaaatct gcaaatcggc gaatgccaag
     5221 ctgcgaactt tacgggcaat gcggtggctg ccaaatgcag catatttcgc ttgaactgca
     5281 acgtaaggca aaacaacagg ctttattcca acgcttacag aaattgcaaa cccacgctat
     5341 tgatgttcag ccgatgatta tcggcaacga taaacactac cgccgtcggg caaaattaag
     5401 cattgcgatc cagcaaggca aggcggtaat cggctttcgt ttgcaaaatt ccaaccaaat
     5461 tataccgctt gcacactgtg aagtgctggt tgaaccgctt gccaatttgc tcccaaaatt
     5521 aaaacacctg attgaaaact ggcaacagcc gaaaaaattg gggcatattg agctggtcag
     5581 tgcggacaat accattgccc tatttttccg ccatttaggg gcattatcgg cgcaagataa
     5641 ccacaatttg cgtcaattcg ccacacaaga gcaattatct ctttttgtga tgactagcga
     5701 aaatcacatt gagcatctgt gcggtgaacg cccgtattat caaattcagg gtctaaagtt
     5761 gcatttttca attcgggatt ttattcaggt gaatgccacg ctcaatgaaa aaatggtgga
     5821 aaaagccctt gagtggctgg aattatccaa ccaagatcga gtgttagatc tcttctgtgg
     5881 aatggggaat tttaccttgc ctattgctga gcgagcgaaa tcggtggtcg gtgtggaagg
     5941 ggtagaacca atggtgcaac aagcccgaga gaatgcggtg acaagcggtc taaaaaatgt
     6001 ggaattttac caaaccaatt tagatgagcc gttttctgac aaaccttggg caaaagagcc
     6061 gtttaacaag gtactactcg acccggcacg caacggagca ctattttgcc tagatcactt
     6121 gacatcactc aatcctgagc ggattgttta tgtctcttgc aatccggcaa cactggtgcg
     6181 agatgccgaa aaactgttgc aactaggcta caggctagaa aaagcggcaa tgattgatat
     6241 gttcccccac accgggcatt tggaatctat ttcgctgttt ttaaaatctt agtagatatt
     6301 gccccgatgg tgcgagcgtc cacgctcgta ccacacaagc ggttaattct gcctaaaaat
     6361 ttgcaaaaaa tgtactatta ttaaccgctt gtagaaattt gttgggtttt cgcaagctaa
     6421 ccccaaccta gttatcgctt ataacacaga aaataataca ccacaccggc taacataacg
     6481 cacaatgcca tcgccaaaag cattggggca gcactggtta cagcaatatg tgaaagccct
     6541 gctccaacaa gggaagcaac accaaaacga gccgtacctg ctactccgtt agccgtgcct
     6601 gccatttgtg gaaattgctc taaaatggcg gcactggcat tactaccaat ggtggaaagc
     6661 attccgatat aaagcggaat accgattgcc attgcccaga agcctaaatc aaaaatcgcc
     6721 actaaagcca gccaaatgcc ggctaacagt tgcatagcta aagcaattcg taacatcgtt
     6781 tcagagccga ctttcactac cattttgctg ttaatcgccg tcatcgtcac catcacaatc
     6841 atattcagcc cgaagaaata gccgaaattt tcttgcgaca cgccataaag cccaatatag
     6901 acaatcgagc ctgaggttaa aaaacagaaa atacctgcaa aggttaagcc gccaatcagc
     6961 acataaccaa gagtaggcct atgtttcagt aagatggcaa aattgcttaa agttttgcca
     7021 aaacttaacg gcaggcgttt ttcgggggct aatgtttcag ggattttcca actaattaat
     7081 accacgcaca aaatccccat cgccactaac acatagaaaa ttgagtgcca gtggaaatat
     7141 ttggcaatat aaccgccaac aatcggtgca agcaatgggg caagcataga aataatcata
     7201 ataaccgaca tcaatttggc aaataaatta cgctcaaaca gatctctcac taatgctccc
     7261 agcaccaccg ccggggctgc cgcacataag ccctgtacta accttagcag gtaaaatgtt
     7321 tcaatattct gaacttgggt cagaaaataa gcggcaatcg ccgagccgac taatccgatt
     7381 aaaataatga ttttccgccc aaagctgtct gccatcggtc cccagaatag ttgccctgcc
     7441 ccaaaaccta gggtaaatac cgccaaggtg gtttgcactt tttcttgcga aaccgccaag
     7501 tcacgggcaa tgtctaaaaa tgagggcaaa tacatatcaa tggctaatgg tggcaacatt
     7561 gccatcatac ctaatatgat gaaaaagctc aggcttggtc ttctactttc cattaaaact
     7621 ggctatctcc tctgaagtga gcgagcggaa ttcaccttct tccaaccttt catctaacac
     7681 cacatcgcca attcgccagc ggtgcaatgc ctcgacttta ttcccaaggg cggcaaacat
     7741 tcgcttaact tgatgataac gcccctcact aatagttaaa ttcacattgt aatcgtctaa
     7801 aatttcaagt tgagccggca aggttggctc tttctcgcca cgcaataaaa ttccttctgc
     7861 caatcgctgt gcataaaaat cttccaccgg atcagcaagc gtcactaagt atgtcttttc
     7921 acaatggtgc ttgggcgagg taatacggtg cgaccatttg ccgtcatcgg ttaacagcac
     7981 cagccctgtg gtatccacat ctaaccgccc tgcggtatgc aatttagtca ttaacggata
     8041 atcaaagaat tgaaacaccg ttgggtactc accatcatcg tgtgaacaaa tatagccctg
     8101 tggcttatag agcataaaat actgcccctc ttccacccac tctaatttct gatcttcgta
     8161 ataaatttca tcttcttgac tgatttttgc cgcaccactt ttttcaatat tgccgtttac
     8221 cgtcaccaca ccacttttca gcactttagc cgcttgtgaa cgggtcaaac cggtattttc
     8281 ggcgataaat ttgtctaaac gcatactatt tttataccat aaattacttt accctctccc
     8341 ctcgtgggag agggacagat ttttcttcgt ctagaagaaa aatcagggag aggggggaaa
     8401 tacgtcttcg tccagaagaa aaatcaggga gaggggaaat gtgcaaactt ttttagaaaa
     8461 atgaccgctt cccctctctc tgcttatttc gctgaacgct cataagcggt ctctctccca
     8521 caaggggaga gagtgtttaa ttgggagatt ataccacaac ttccctaaca aattcgcttt
     8581 ctagcccgac caacggcaag ctagagtcgt gccaatctcg caataattgg tgttgcatta
     8641 aatttaacgt atccaacccc ggcacactgt ttggggtgta ttgttgggcg atacgagcca
     8701 gttgggttaa gcccaaactg ctctcaagac ttgaactaat caccgctact aatccttgct
     8761 tctgtgcttg ttgaattaaa ttcacgcatt tttcaagcga accaaccagc gtaggtttaa
     8821 tgataatagc ggtcaaattt ggctcttttt ttaccaaaaa attcgcctct ctcacggttt
     8881 cgtcccacgc aatcgcaatg tcggtttgtg tggcaaattg gcgggaaagt tcgagcgctt
     8941 ggcaaggttc ttcgataaat tgaatacggg gtttatgttc atttgcaatt ttttcagcaa
     9001 atttgaccgc ttgttccaag ctccaaagac ggttggcatc aagccttagg cgtaaattcg
     9061 gaaaagtatg caacagcaaa tttgcctgct cgccttcctg ttcaggattg agaccgattt
     9121 taattttacc gattgggttc gtatgttggc taaatgccgt gtgtaacttt tccaaatcgc
     9181 catagcacag caccgccgat tggttattcg cttgctcgcc taattcatta ttcagttcag
     9241 ccaacgcaca gcttagccca aaggcaacag acgggaaaag ctgctctaac gtttcaggct
     9301 ctcctttctg ccacgccttt aaccaattca cagcttggtt ttcagcttgt tctaggctct
     9361 cttggctaaa acttggaagc ggtgcaattt cgccccagcc gactttctcc tgttcttgca
     9421 aacacaccaa caccccttgc ctttctttca aaggttgctt gcgtaacact acaccggttt
     9481 gaatggggag ttggtagcgg tataacttta cttgtcgcat actattttta tccaaaatag
     9541 gtttagaggc gaaacatctt tcgcccacat catcatttgg gaaattgagg cgaaaaatgt
     9601 ttcgcctcta gcgcatcata ttctaccgct tgttagccca aagctctaat aaattcacgc
     9661 caactggtaa acctgccact tttgggctaa aatagacggc aatttttacc ctaattttaa
     9721 tgagaaaaag aatggctaac caaaaacgaa aaatgcttgt tacttgtgcc ttgccctatg
     9781 ctaacggtgc aattcattta ggtcatatgc ttgaacatat tcaagcagat atttgggtgc
     9841 gttttcaacg tatgcgtggc aatgaagtcc actttgtctg tgctgatgat gcccacggca
     9901 cgcctattat gcttaatgcc gataaattag gcattacccc tgagcagtta atcgaaaaag
     9961 ccaaagccga tcacgaagcg gattttgcca gttttaatat cagctttgat aattaccatt
    10021 ccacccacag cgaagagaac cgccaaatca ctaccgatat ttataaaacc ttaaaagcga
    10081 acggttttat taaaagcaaa gtgatttcac aactgttcga cccagaaaaa aatatgttct
    10141 tgccggatcg ctttgtgaaa gggacctgcc cgaaatgtaa agccgaagat caatatggcg
    10201 ataactgtga agtatgtgcc tctacctata gcccgatgga tttaatcaac cctcgctcgg
    10261 cagtttccgg tgcaactccg gtggtaaaag aatcagaaca tttctttttt gacctgccaa
    10321 gctttgaggg aatgttgaaa gagtggactc gttccggctc actccaacct gaaattgcca
    10381 ataaaatgca agaatggttt gaaagtggtt tacagcaatg ggacatcagc cgtgatgccc
    10441 cttatttcgg ttttgaaatt ccggacgcag aaaacaaatt cttctacgtt tggttagatg
    10501 cccctattgg ttatatagca tcgtttaaaa atttatgcga ccgtgcaggc attgattttg
    10561 atgagttttg gcaaaaagat tccaccaccg agctttatca cttcatcggt aaagacattg
    10621 tttacttcca cagcctgttt tggccggcaa tgttggaagg cagcggctac cgcaaaccga
    10681 ccaatgtgtt tgctcacggt tatgtgacag tggacggggc gaaaatgtcg aaatctcgtg
    10741 gtacattcat tcaagccaac acctatttaa aacatctcga tccggaatgc ttacgctatt
    10801 actatgcggc aaaattaaac gatcgcattg aagatttaga ttttaattta gacgacttcg
    10861 tgcaacgtgt aaacagcgat atcgtaaaca aattagtgaa tcttgcttct cgcaacgcca
    10921 gttttatcgc taaacgcttt gagggcaaat tagctgaaaa attagatgac gaagcgttat
    10981 ttaatgaatt taccgctcaa gcagaaacca tagcgaattt ctatgaaaac cgtgaattta
    11041 ataaagctat tcgtgccatt atggaattaa ccgataaagc caataaatac attgacgaca
    11101 aagccccttg ggtcatcgcc aaagaagaag gcaaagaggc tgaattacaa gcggtttgtt
    11161 caatgggcat tgagttattc cgtgtgctga tgagctacct aaaaccggtg ttaccaaaac
    11221 tggcagaacg tgccgaagca ttcttacaaa gcgaattgcg ttgggataat atcgctaccc
    11281 cattattcgg gcataccgtg gcaccgttta aatcgctatt ctcacgcctt gagaaaaaac
    11341 agattgatgc agtgattgat gaaaccaaag ccttatttgc cgaagcgaat aagaaaaccg
    11401 aaaaccaaac caaaggaaca caaaaagtgg aacaacaagc ggtcgaaaaa caagaaaata
    11461 ttgcagttga gccaatcagc cctgaaatta cgattgatga tttcgcgaaa ttagatttac
    11521 gtgcagccaa agtattgaac tgcgaggctg tgccaaaatc agacaaaatg ttacgttttg
    11581 agctggattt aggcgaccac caacgccaag tattctcagg cattaaggcg gcctacccaa
    11641 atccggaagc acttatcggg cgttttgtga ttgtaattgc caaccttgct ccacgcaaaa
    11701 tgtcgttcgg tatgtcggag ggaatgattt tatctgccgg cacaggcggt gcgaacctgt
    11761 tcttattaga tgcagacagc ggtgttcagc cgggaatgca agttaaatag gagaagatga
    11821 tgattcaaca aacgctttct attattaagc cggatgctac acgccgtaat ttaatcggca
    11881 aaattttggc tcaattggaa gaaaacgggc tgactatcaa agctctaaaa aaagtacatc
    11941 ttacccgtga gcaagcggaa ggattttatg cggaacacaa aggcaaagat ttctttgagc
    12001 cgttagtgga atttatgaca tcaggatcta ttgtcgtttc tgtactggaa ggggaaaatg
    12061 caattagcca ttatcgtgca ttaatggggg caaccaatcc ggataaccgt gaaaaaggca
    12121 ccattcgcga tttatatgcc ttaagtatgc gtgaaaattc ggtacacggt tcagattccg
    12181 aagagtcggc aaaacgtgaa attgcctact tctttgtacc gagtgagatt gtttaataaa
    12241 gtaggggcga aaattttttc gccccactat tgtaattcgc cctagcgtag attaaatata
    12301 ttctacgcca acaatgtcaa actcaacttt gccacccggt gtggtaatgc tgactgaatc
    12361 gtccacctct ttgccgatta aaccacgagc aatcggggaa ttaactgaaa ttaagccctc
    12421 tttaatattg gcttcatcat cacccacaat gcggtaagtg acttcttcat ctgtatcagc
    12481 attcactaac tgcaccgttg ccccaaaaat cactttgccg ttgtttgcca ttttagtgac
    12541 gtcaataatt tgagcattgc ctaatttgcc ttcaatttct tgaatacgcc cctcacagaa
    12601 accttgttgt tcacgggctg cgtgatactc cgcgttctct tttaaatcac cgtgttcacg
    12661 ggcttctgca atcgcatcaa taatttgtgg gcgacgcaca ttttttaaaa aatccaattc
    12721 ttcacgcagt tgctctgcac cacgcaccgt cataggaatt tgtttcatta aatctatttc
    12781 cttactaaaa attatcggtt tacgaattaa aacgaaaggg ctattattca atttctcact
    12841 aaaaatttca actccccttt tattatgatt tttaaaaatt tcactcgt
//
LOCUS       DANLUA010000064        12768 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLUA010000064 DANLUA010000000
VERSION     DANLUA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..214
                     /locus_tag="PXB44_001254"
     CDS             <1..214
                     /locus_tag="PXB44_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5281733.1"
                     /translation="ILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGS
                     RIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            257..586
                     /locus_tag="PXB44_001255"
     CDS             257..586
                     /locus_tag="PXB44_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5281734.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(625..1011)
                     /gene="cueR"
                     /locus_tag="PXB44_001256"
     CDS             complement(625..1011)
                     /gene="cueR"
                     /locus_tag="PXB44_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL5281735.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            1100..1300
                     /locus_tag="PXB44_001257"
     CDS             1100..1300
                     /locus_tag="PXB44_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5281736.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            1353..3545
                     /locus_tag="PXB44_001258"
     CDS             1353..3545
                     /locus_tag="PXB44_001258"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL5281737.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3598..4383)
                     /gene="truA"
                     /locus_tag="PXB44_001259"
     CDS             complement(3598..4383)
                     /gene="truA"
                     /locus_tag="PXB44_001259"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL5281738.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(4448..5137)
                     /gene="folE"
                     /locus_tag="PXB44_001260"
     CDS             complement(4448..5137)
                     /gene="folE"
                     /locus_tag="PXB44_001260"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL5281739.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            5238..5945
                     /locus_tag="PXB44_001261"
     CDS             5238..5945
                     /locus_tag="PXB44_001261"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL5281740.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(6000..7097)
                     /gene="apbC"
                     /locus_tag="PXB44_001262"
     CDS             complement(6000..7097)
                     /gene="apbC"
                     /locus_tag="PXB44_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL5281741.1"
                     /translation="MNQLNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(7094..8641)
                     /locus_tag="PXB44_001263"
     CDS             complement(7094..8641)
                     /locus_tag="PXB44_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5281742.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(8667..9317)
                     /locus_tag="PXB44_001264"
     CDS             complement(8667..9317)
                     /locus_tag="PXB44_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL5281743.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(9439..11310)
                     /locus_tag="PXB44_001265"
     CDS             complement(9439..11310)
                     /locus_tag="PXB44_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL5281744.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            11492..11761
                     /gene="rpsO"
                     /locus_tag="PXB44_001266"
     CDS             11492..11761
                     /gene="rpsO"
                     /locus_tag="PXB44_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL5281745.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            11833..12702
                     /locus_tag="PXB44_001267"
     CDS             11833..12702
                     /locus_tag="PXB44_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL5281746.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
BASE COUNT         3654 a         2691 c         2527 g         3896 t
ORIGIN      
        1 aatcctaaag cggaaatctc aaaagacact acaacaaatc tacacccagc ctatttctgc
       61 caatattaaa tggacaaata tagaagctct ttttctagag ttaggagcag aaatagaaga
      121 acgagaaggg agcagaattg cagttgtttt atttcaacag gtcagagtat ttcatcgccc
      181 acacccgaat cctgatacag ataaggggcg gtagcgagta ttaaaaaatg gttatctgaa
      241 aacggagtca ttccttatgc gtaatattat ggatattaat ggttataaag ctgtcattgc
      301 ttacgatccc gaaacagaat tatttcgagg tgaatttatc ggattaaatg gtggagccga
      361 tttttatgcc gataatgtca ttcaattaaa aaaagaaggt gaactaagtt tgaatatctt
      421 tttagaactt tgtagagaaa aaggtattga gccgttcaaa caatattccg gaaagtttaa
      481 tgttcgcctt tcgcctgaat tacataaagc tgcggtaatt gcagcaactg ctgaaaattt
      541 aagcttaaat gaatggatta accaaacgtt agaaaagagc gtgtagccta attgcaaaac
      601 aatcacctat tttgaccgct tgtattaccc gtttaaccca ttcaatatcg aacactccga
      661 attggcgttg ccacagcaag agctactcca gcttttgagc aaagtcagca tttggtttaa
      721 atcggcaatt ttttgttcaa gctcaccgat atgctgttcg gttaattgtt ttacatctcg
      781 gcttgagcgg tttgggttgt cgtttaattt aagtagttcg ctgatttgag ccagcgaaaa
      841 gcccactttt cgggcattgc tgataaaatg taatcggctc aaatcagtag cattgtagaa
      901 tcgatacccc gcttcggtgc gacttgtcgg cggcagaagt cctgcttttt cataatctcg
      961 gatttgtttg gcggaaagcc cggttttttc tgcggcttgg ctaatattca ttataatcct
     1021 ctcactttaa tcgcttgacc ttaacctaag gttaagcttt attatacgcc tgaataatca
     1081 ctcaaaacag gagaaaatga tgacaattac cctacaactt gatggcttac attgtggcaa
     1141 ctgtgtgaaa agtgtcgaaa aagcgttaaa tggcgtggct ggggtgaacc aagttagcgt
     1201 aacgttagaa ccgcaaattg ctgtggtgga aggctcagca gaagcacaag ccttaattga
     1261 agcggttgaa gacattggct ttgacgcaaa attagcctaa ttaataaggg gcagaaattt
     1321 tgccctaatc tgcccaatat ttcggaggat aaatgacaag cacagctcac tattcgccag
     1381 aacaacaact tttgattgat ggaatgcact gtgccgcctg tgttcggcga gtggaaaaag
     1441 ccttacttaa agtggaaaag gtggaatttg cctcagtcaa tcttgccgat caaaccgctt
     1501 ttgtgcaagg caacgccaac cctgaagcct tggtagcagc ggtagttaaa atcggttttg
     1561 gggcagaagt gctggaaagc gaggaagaac gccgaattaa acaacaagcc caaacgcaac
     1621 gagtgttagg gcataaacgc actcaattta tgcttgccct tgtagttggt tttgccttag
     1681 ttgcttttgg gttctacaaa ggaatgacgg taaacgaaac caatcgttcg ctttggctca
     1741 gctgggcagg tattagcctt gcgacaatgt atttttcagg taaagatttc tttcttgggg
     1801 cattttcagc actgaaaaac cgctcagcca atatggacat gttagtagca atcagcaccg
     1861 gcacggcttg gctctattct tgttggctca cgcttttccc acaaccgaat gctcacgttt
     1921 attttgaagc cagcgtgatg attctcggct ttatcaatct aggaaaatac ttagaactga
     1981 aagccaaaca gcgttcctcg ctagcattgg aaaaattgct ggatctcgcc ccaaaacaag
     2041 cggtcatttt tgaagaaaat attgcaaaaa ccattccggc taaagcaatt aagccacaaa
     2101 tgcgggtgca agccttaacc ggtgaccgct tggcggttga tggtatttta gacagtggct
     2161 ctatttgggt tgatgaatca atgctcaccg gtgaagccct acctgtagag aaaaaaatcg
     2221 gtgataaagt gcgagccggc actctggtgc aagacggttc aggcacttac attgccgaac
     2281 aggtcggctc acaaaccgct ttggctcgtg taatcaatgc cgttcgccac gcccaaagca
     2341 gcaaaccgcc attggcacag tttgtagata aaatcgccgc tgtttttgtg ccggtggtgg
     2401 tttcaatcgc attactttct gcgttaattt ggctggcagt tgggcaggat tttgcctttg
     2461 cactctcgat ttttactacc gtgttgatca tcgcttgccc ttgtgcattg gggctggcaa
     2521 ttccgctttc taccattgcc ggcgtggcac gttctgccga atttggcgtg ctggtgcgaa
     2581 atattgaggc actacaagca agcagtgaaa tcgacaccct agtgttcgat aaaaccggca
     2641 cactcaccac tggcagaatg gaagtgtcgg atgttctcac ctttaacgga tttgagcaaa
     2701 agcgcttgct acaacttgct aaaagtgtag agcaacacgc ctcacacccg attgcgaaag
     2761 cgattgcaaa atttgccgaa aatcagaccg cttgtggtgt aaccgatgtg caagtgttga
     2821 aagggttagg catttcagca aaatatgaag gcagtacaat cagaatggga aattggcaat
     2881 ttgtaggcga aaccattcaa gcaaatcttg cctccctatt gcccgctaaa gagggggatt
     2941 tttcctcaaa aatttttgtt tcggtggata atcaactcgc cggtgtattg tcggtttcag
     3001 accaacttcg ccctgaagcg aaagcaacca ttcatcaatt ccaagcggaa ggctatcaat
     3061 gcgtgatgct gaccggtgat cgccaacaaa ctgccgacta tttcgccaac gcattggggt
     3121 tagacggcgt gattgcggag gtattaccgg agcaaaaagc ggaaaaaatt cgagccttgc
     3181 aagcagaggg taaaaaagta gcaatgattg gcgacggcat aaacgatgct ccagctctgg
     3241 ctcaagccaa tgtgggcatt gcgatgcaca atggttcaga tattgcagtg gaaaccgccg
     3301 atctttcgct gatgcgagaa gggctagcac cactgatgca aattctaccg ttctctaaaa
     3361 aagtgctgaa aaatatgaag caaagtttgc tcggggcttt tatctataat gttattgcga
     3421 ttcctattgc tgccggcgtg ctttaccctt tcaccggctg gctactcaac ccaatgattg
     3481 ccgccattgc gatgacattg tcgtcaatta ccgtggtgtt aaatagtcaa aggttgttga
     3541 aataatttaa gcggtcaaat ttgcatattt ttttgcaaat ttgaccgctt gtaatcacta
     3601 atccgccaaa aacagcggcc caagaggtgt tttcggaatg ccaaaatgct cagggtaatg
     3661 cacctcgacc aaatatagcc cctcggcttt cgctgtaggg gcagcttttt cccggtcacg
     3721 ttgctccaac acccattttg cccattccac aggctgtctg ccttgcccga tttcgagcaa
     3781 tgttcccaca atattgcgaa ccatatgatg tacaaaggcg ttggcttgaa tatcaacaat
     3841 cacataattg ccttgccgag acacatttaa atgatgaata ttccgccacg gcgtattaga
     3901 ttggcatttg gcggcacgga aagaggaaaa gtcattctcg cccagcaaat actgccctgc
     3961 ttggtgcatt ttgcgttcat caaggggaaa gtgaaagtgg gtaacacctt taggtaaaat
     4021 tgccgaccgc agtttttggt taaaaataat atagcggtaa cggcgggcgg tggcactaaa
     4081 acgggcgtga aaatcctcat tcaccgctac cgcccatttc accgcaatat catcgggtaa
     4141 atgagcgtta gtgccgaaac accagctttg tagcgggcgg tttgcttgcg tttcaaaatg
     4201 cacgacctgc cctgtgccgt gcacaccggc atcggttctg ccggcacaaa acacttctac
     4261 gggcgaattg gcaacaaccg acaacgcctg ctctagctgt tgttgtacac tctctaccgc
     4321 ttcttgtcgt tgccagccaa aataacggct gccgtcatat tcaataccta atgcgatttt
     4381 cattttaata ccttgataaa aagaggggcg aaaaattttt cgcccccaca gtaattgata
     4441 tttgcaatta tttattcaat aagcctaaaa attctttgcg ggtttctcgg tcttctaaga
     4501 aaacaccgcc aaaggcagaa gtaactgtat agctattggt gtcttttatg ccacggcatt
     4561 tcacgcaaaa atgggtggct ttcacataca ccgcaacatc gtctgtttct aaaatagttt
     4621 gaaaggcagt taaaatctgt tcggtaaaac gctcttgcac ctgcggacgt tgggcaaaaa
     4681 actgcacgac acggttgatt ttcgagagtc caatcaccca ttttttcggg taataggcta
     4741 ccgctacttt gccgtcaata gtaacaaaat gatgctcaca ggtagaagtt aaagtaatat
     4801 cgtccaccag caccatttca ctgactttca ttcggttttc aatattggta attttcggga
     4861 aggtagcata atcaagcccg ctgaaaattt catcaacata cattttcgcc aaacgatgag
     4921 gcgtttcttc taagctgtca tcacttaaat ctaaaccgag caattctaaa actgcctgca
     4981 tatgctgctg gatttctgct cggcgactgg ctttatcttt ggtatgagta acagtcggag
     5041 tttcaatgcc ttttgccaac aaggcttggc gaactttttg agcctccggc gaaagcgtgt
     5101 tttctgtgat agttaattcg ggcgagatcg ttgtcattgg ttttcctctc aaatgtaagc
     5161 tgaagattct aacaaattga catcaaaaaa aatagctcta aaatgagcta tttcagccat
     5221 aaacaccctc tttatttatg caaaatatac agtatctctt taatgccgac ctgattcccg
     5281 aacatcaagc tgaatttgtg cagtctctct taacttgcaa aaattttgac aattccgacc
     5341 gcttgctagg cttctcaaaa gggcgaggta cgacttggtt tttagatacc tctgctgaac
     5401 taggtatcaa tgtggtgctt cgccattatt atcgtggcgg cttgttcggt aaaatcatca
     5461 aagatagtta cttttttact cgctttgagg ccacccgagc gtataaagaa tttttcttac
     5521 tgcaacaaat gttagcttgg ggtttacctg tgcctcgccc tgtggcgatt aaaatagaaa
     5581 gacatttttg ttgctatcgt gccgacatta tgctcgaaaa gctagaaaac acacaagatt
     5641 taagcaaaat cttgcaatcc cattctttat cgaacgaaca atatgagcag atagggaaac
     5701 tgattcgcag tctgcataac catcaaattc accattccga cttaaatatc cataatattt
     5761 tgcttggtaa tgatggcaaa ttttgtctga ttgattttga taaatgcggc attgagccgg
     5821 gcgaaaattg gaagcaaaat aacctagacc gcctgttacg ctcgtttaag aaagaacaag
     5881 ggcgattaaa tattttcttc agcgaagaaa attggcaggc tgttttgaga ggatatactc
     5941 tctaagaaac aataatggca caagcttaga aacttgtgcc atcaaatcat caatattaac
     6001 tacttcactt cacgaatcat aatttcagac ggaatcacac tgccttgcca gtaaagttca
     6061 gaagctactt tttccgctaa tgcgaggtag gcttggctga tttcgtgttc cggagctgcc
     6121 accaccgttg gcacaccgct atctaaatct tcacgcagac gaatatgtag tggtaattgt
     6181 gctaacactt tagtgccata tttttgggcg attttttccg ctccgccggt gccgaaaatt
     6241 gcctcatggt gtccgcagtt tgagcagata tgcattgaca tattttcaat caccccaagc
     6301 actggtactg agactttctc aaacattgag atccctttaa tcgcatctaa taaggcaata
     6361 tcttgtgggg ttgttactac caccgcaccg gtcaccggaa tttgctgtga aagcgtaagc
     6421 tgaatatcac ccgtacccgg tggcatatca atgaccaagt aatccaattc attccaccaa
     6481 gtttcattta aaagctgact taacgcactg cttgccatcg ggccacgcca aatggtggcg
     6541 ttatcttctt ccattaaata gccgatagaa ttagattgca agccgtgagc ggtaatcggc
     6601 gtgatgtgct tgttatcagg agaagttgga cgttggtctt ttgctcccaa catatgcgga
     6661 atagatgggc cgtaaatatc cgcatctaaa atccccactc ttgtcccttg tgctcgtaag
     6721 gctaaagcaa gattaactga ggtcgttgat ttccctaccc caccttttcc tgaagtgacc
     6781 gcaatgatat tttttacccc attgactgcc gggtgtttat tcgcccgttt taaggtcgca
     6841 atttggtaat ttaataccca ttttacaccg cttgaacctg ttacttgctt taatttagcc
     6901 tctgtttcgg cttttaaggt ttcaaaacca ctgttccacg caaatggcat cgtgatttca
     6961 attctaagaa tatcgccccc taattctgct tttttaaacg cattaagggc aattaaatct
     7021 ttctgtaaag tcgggtggga aaattgttgc aagattgctt taatttcaac aagttgctgt
     7081 tcatttaatt ggttcattgg ttatccttgt ttgctaaatt gcgttttaaa tctttgctat
     7141 aaattacttc gctatcgtta ttcaaatcaa tacccaaaag ggaggaaaca atcccaaccc
     7201 gatgatggta ttgaaataat ggcaatacgg caacatcgct ataaagctgc tcgctaattt
     7261 cttgaataag tgactctctc gcttctgcac taagcgtttc tgtttgtaaa cgatttaatt
     7321 tttgatcgac tatctcactt ttatagccgg aatgattatc cgggctcatt gaatgaaact
     7381 tctgcaaaaa tacaatcgga tcaggataat ctgcacacca gcctgctcgt gctatctggt
     7441 aatctttttg ttcccttact ttatttaaag tctgccaatc aaccatttta ggtgtaatat
     7501 taaataattc tgattgagaa agggctcgag taacttggtt agcaatatta atgctattct
     7561 catcatataa caaagtcagc tttaaaggat tataagggct aattcctgct tttgttaata
     7621 gctgctcaac cacaacagga ttccaacgct tttctctatc ttttactaag ctatgtggaa
     7681 ttacagactg actcggaacc ccatattctt gcccactatt tgctgaagcc accattgtcc
     7741 ttattgcttc acgaatctct cgtttagcta ataatggatc atcaaaattg aactcatagt
     7801 aataagagca caaacgcgga aatttcacga tattcgtttt tttatttaat aaaggattct
     7861 cgacaatatc aaaagcagag cagttttgga tagttgtaat taaatcatac tctatagttt
     7921 gaaagggaat ttcttgggtt cgagccttta acaccatttt ttgagcatct agtttctcga
     7981 gacggtaaga cgcattgctc acgaagttat ctatggttgg agcaatccct tgataagtag
     8041 gtaataatgc ggaatgtgcc agcatttcag gcaattggtg atttgggtga tttagccgaa
     8101 ttctcaatgt atgcgcattt aatgcctcta caccgagttc ttcaactgac tttatttgct
     8161 cttcaatcgc ttttgcattt tctataccca tatagatgag atatttagac aatacagaat
     8221 gattttgagg atctattaaa cgtcgccagc ttgctacaaa atcttgtgca gtcacaggtt
     8281 ggccattaga ccacacagct ttctcatcta aaataaacaa ccactctttt tcatcttcag
     8341 taaaccaatt ctgagcaatg gccggaacga catcaccttt tctgttgtag gccgttaaac
     8401 cgactaatag atctcttaaa ggagcagcat ctgcagaagc tttaatttta tgtgggtcga
     8461 gttgaaatac agcagaataa attgcacgtt tgagtaaagt atcactctgc cggttttcta
     8521 cccagacaga ttgaatggat tgagggcaaa ttaattgatc acaagatgta ataagcagaa
     8581 tgcaagcggt aacttttgca atttttttta caaatgtaac cgcttgtaag gatttcaaca
     8641 taaactcccc aaaggttaat taagatttat tttacaagaa gcacaggaaa taaagttgta
     8701 attcctgcca caataatttc aattcccagc gccattaaaa tcagccccat gatacgagta
     8761 acgacattag atcctgtttt gcctaatttt ttcaccaaag gggctgaata gcggaataaa
     8821 atataacaaa ttaatgcaaa tacaataatc gcaatgctaa agccaacata gtcttcccaa
     8881 gtatggtagc gggttcccca cacaatggtt gagccaattg cccctggtcc tgccataatc
     8941 ggcattgcta atggcacgac accgatattt tcgtattcag taatatcttc atttttctct
     9001 tctttatttt gtttgtgttc acctaattta ccgctaatca tcgtcatcgc aatgcttaca
     9061 atcaaaatgc caccagctac tctaaaagag ctaagtgaaa tgctaaacgc attgagaatc
     9121 agattaccaa aatagaggct gactaataaa attactccaa tagaaacagc ggtgattaag
     9181 ctggtgcgat ttctttcccc ctcatattgg tgagcggtca tactgaaaaa aataggcaag
     9241 gaaccaaaag gattgactat agcaaataga ccaatgaaga attggaggta gatggcaaaa
     9301 ttaataatga tatccacaaa aactttctca aacaacagtg tttcgggcgc tattatatcg
     9361 gttaatcaag gtagatacaa accaaaagca cccagcctaa aggacggttg ggtgcttttt
     9421 gataaaatga aaggcttgtt agtgtgacga acgttgctgc ataaaatctt catttacctc
     9481 aattgaagct tttgcacgta gcgattgtaa gaattcatta cgtaaaatta aacgattagc
     9541 ttgttcaaat tgtggcgcca atgtcttaaa ctgttcagca ttcccttccg taatgttatc
     9601 taaggcaaca ataactacat cgcccttttg attacgtgcc atttgataag tcgttttatc
     9661 ttgcgccttt ttcattgcga aaatcgttcc agccaatacc gggttttcta cttctgcttg
     9721 catataaact aaagtttgtg gcgcattaaa tttcacgtcc actgatttcc cttcattcaa
     9781 ggcttttaca ttttcttcta ctttcgccag caacgctttt tccgcttttt ctgccttcac
     9841 aaaattttca actgctgctt ttgcttcatc aaaagtttgt ggacgttctg catcataatc
     9901 actcactctc acaaacaatg tctgtggatt tgcatcattc ccaacatcta aagcatcaga
     9961 attttgttta ttttggcgta gctctccttc aaacaaaact tttaatactt tctcattttg
    10021 taaagcagta ggtacgtttt cacgggtaaa cagatccgtt ttttgcagtt ccacattacc
    10081 ggctttcgca acttcttcaa gtgaagaatt attctcaaag gctttatttg ccatttcgcg
    10141 cgcaactgta aaatactcag ttaacactaa ttcttggcga atagtttggg caattttatc
    10201 tttcacttgt gctaactcaa tcacttgctc aggtttacgc tccaatactt tgatgatatg
    10261 gtaagcatta tccactttca ccgctgcact catctggccg gcagttaaat ttgctacagc
    10321 atcttcaaaa gctttcggga aagtgccagc ttttgcccaa cctagatcac caccattatt
    10381 tgctgataaa gtatcgctag atttgttttt tgccaaagta gcaaaatctt ccccattttt
    10441 gagcgcttgc tcaatagcct gtgcttccgc ttcactagcg acttgaatat gagctaaatg
    10501 ggcttcacct ttagttacat aatcggcttt attgcgatca tagtaagttt gaatttgctc
    10561 attggtaatc tcaactttgc tttccacatc ttttggagaa agagtaacgt actcaaccgc
    10621 taatttttca ggtgctaata atttagtttt atttgcttcg taataggctc gcaattcttg
    10681 ctcggaagcg gtctgatttt ccatttcttt tgcaactgaa aattcggcta aacgcacttc
    10741 acgttgttgc aacagtaatt ttgctaacaa tgcctgctgt gccggtacat taaaatcact
    10801 attgataatc ccctcttgta gctgggattg caacatacct tcctgcacaa tcgctgcata
    10861 gccatcggca cttaaattat tgtggcgtaa tgtttgttgg tataactcgt tactgaattt
    10921 accatcttgt tgaaacatct ggctgtttac aatttcagat ttgatttgat cagcactaat
    10981 acctagtttt aactcttttg catactggcg cagaagctct tcatcaatta atccatttaa
    11041 taccgattga ttaaattgag cagcatactc cgggctatcc attaaatccc aaaagcgact
    11101 ccccagttca gcatttaatc tattttgctc acgattaagc gtgttattaa ataattgctg
    11161 actaatttct tccccattta ctttcgctac agaattatct tgtgaaatca atcctgtccc
    11221 aattccaccg attacaaatg aaatagagac taatgcaaaa ataattttaa agaccggccc
    11281 atttgtccgc tcgtgcattt tttcaatcat ttaaaaattc ctaaattact caaaaattgt
    11341 cgctgattat actctttctc aaatgagaaa taaacttgca aatttttatt gaaatttaac
    11401 cgctttctct ttaaaattca cagtccctgt cgtagctgag ttagagatcg gctattgatg
    11461 tattatcaac ccttaacaaa aggaaattaa aatgtctcta agcgtagaag caaaagcaaa
    11521 aatcgttgct gaatttggtc gtgatgcaaa agacactggt tcttctgaag ttcaaattgc
    11581 attattaact gcacaaatca accacttaca agcacacttt gctgagcata aaaaagatca
    11641 ccacggtcgt cgtggtttat tacgtatggt ttcacgccgt cgtaaattat tagactactt
    11701 aaaacgtact gatcttgcta aatattcgga aactatcgca cgtttaggtt tacgtcgcta
    11761 atttcaaata ttagatatga gagccgagct gaaaagttcg gcttttttat aaattttatt
    11821 cgaggaaaaa atatggcttt tgcgtcatta tttacattat tagacgatat tgctgccgtg
    11881 cttgatgatg tatcgatcat gactaaagtg gctgctaaga aaacagcagg cgtaatcggt
    11941 gatgatcttg ccttaaatgc taatcaagtc agtggaaaga atgtggtgcc agaacgagaa
    12001 ctaccgattg tttgggctgt agcgaaaggc tcattaatca ataaagtgat cttgattcca
    12061 ctagcgttgc tgctatcggt ctttttaccg caggctatta tcccgctatt aatgataggc
    12121 ggtgcatatt tatgttttga aggcgtagag aaattactcc atcgtctatt acataaaaac
    12181 aatcaagctc attctgatga aaccgaaacc ctttctgaaa aagataaaat taaaggtgca
    12241 attcgcaccg attttattct atcagcagag attatcatta ttgcgttagg tgttttgcaa
    12301 gactcttcaa ccctaaccca aattttatct ctctccgcta tcggggtcgg aattaccctg
    12361 tttgtttatg gcttagttgg attaattgta aaactagatg atatcggttt atggttaatg
    12421 cagaaaaaaa gcacattctc acaagcggtt ggaaaaggct tattatttgc aatgccgtgg
    12481 tttatgaaag gactttctgt gattggtact attgcgatgt tcttagtcgg tggtggtatt
    12541 tttacccata atttaccaat gattcatcac ttcgtagaac aattcggtat ctctccccat
    12601 ttttctactc tggttgattt tgcagtgggt attgtggttg gtattattgc atgcttaatt
    12661 attttacctt tgatgaaatt attttctaaa aaggcgcact aatccccaaa agcgtagctt
    12721 tggttaaagc tagcttttta tctcttgcta taaaaaaata gcaggttt
//
LOCUS       DANLUA010000065        12737 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLUA010000065 DANLUA010000000
VERSION     DANLUA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            288..506
                     /locus_tag="PXB44_001268"
                     /pseudo
     CDS             288..506
                     /locus_tag="PXB44_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(558..773)
                     /locus_tag="PXB44_001269"
     CDS             complement(558..773)
                     /locus_tag="PXB44_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281747.1"
                     /translation="MQTEKRGRGRPKSGLTLQELQAKSDAKRGVRLKSFKFHEDFIAQ
                     LEQLAEQHGISQTQVIVQAVEQFSKGA"
     gene            1318..2019
                     /locus_tag="PXB44_001270"
     CDS             1318..2019
                     /locus_tag="PXB44_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281748.1"
                     /translation="MSTQISTQTISFNNQALITFEQNGTHYTAMKPICENIGLTWHAQ
                     YERIKRDEILSQGILIIRIPTNGGDQQMICLPIEYLNGWLFGIDINRCKPEIRETLIK
                     YKKECYQALHDYWFKGKAERSQIAPIVEPKIPMELTKKEWLRFASMWYALYNSLEVLA
                     TLEKPLRAIGSPFGATAYTHATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTT
                     LRQYQPKGLGGLVRI"
     gene            2273..2449
                     /locus_tag="PXB44_001271"
     CDS             2273..2449
                     /locus_tag="PXB44_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281749.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
     gene            2537..2920
                     /locus_tag="PXB44_001272"
     CDS             2537..2920
                     /locus_tag="PXB44_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281750.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEEDQIRCPKTYAKL
                     REILEANEENVVRIGNVSISIGSTT"
     gene            2970..3191
                     /locus_tag="PXB44_001273"
     CDS             2970..3191
                     /locus_tag="PXB44_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5281751.1"
                     /translation="MNIYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFP
                     VVRRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            3213..4253
                     /locus_tag="PXB44_001274"
     CDS             3213..4253
                     /locus_tag="PXB44_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5281752.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRANGSIVEYYFYVLADGKERSL
                     GTNKHEAILEAAKLNFENSRKSPIVLFIDVAKRYELEVVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTALPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHRSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IRERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(4412..4699)
                     /gene="rplY"
                     /locus_tag="PXB44_001275"
     CDS             complement(4412..4699)
                     /gene="rplY"
                     /locus_tag="PXB44_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL5281753.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(4851..6119)
                     /locus_tag="PXB44_001276"
     CDS             complement(4851..6119)
                     /locus_tag="PXB44_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL5281754.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(6276..7187)
                     /locus_tag="PXB44_001277"
     CDS             complement(6276..7187)
                     /locus_tag="PXB44_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL5281755.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(7184..7903)
                     /gene="nfsA"
                     /locus_tag="PXB44_001278"
     CDS             complement(7184..7903)
                     /gene="nfsA"
                     /locus_tag="PXB44_001278"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL5281756.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(7915..8700)
                     /gene="mtgA"
                     /locus_tag="PXB44_001279"
     CDS             complement(7915..8700)
                     /gene="mtgA"
                     /locus_tag="PXB44_001279"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL5281757.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(8675..8995)
                     /gene="trpR"
                     /locus_tag="PXB44_001280"
     CDS             complement(8675..8995)
                     /gene="trpR"
                     /locus_tag="PXB44_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL5281758.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(9075..9716)
                     /gene="lolA"
                     /locus_tag="PXB44_001281"
     CDS             complement(9075..9716)
                     /gene="lolA"
                     /locus_tag="PXB44_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL5281759.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(9827..12685)
                     /gene="polA"
                     /locus_tag="PXB44_001282"
     CDS             complement(9827..12685)
                     /gene="polA"
                     /locus_tag="PXB44_001282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL5281760.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         3809 a         2831 c         2390 g         3707 t
ORIGIN      
        1 ccggtcatca acttttgaca aaatacactc aacgccacag ctattaatga atggcgagtc
       61 ctgattgatt agccattgtt tgaaaagctc tttcatagtg ttctccttat atttttttat
      121 gagattacta cgaaagcgtt ttactgtcag aacaaaaatt tattttttcg atgcagatcg
      181 caaaaataga tattgaggct tgacaccgcc aaacttcgga ttaagataac cgcactttca
      241 agccgtccac caacggcttt ttttgtacct aaaaacggag aaacgaaatg gcaaatagcg
      301 taatgattgt acaaggcgtg gaggtcaaag taaccgctcg tgatggtgag gattacatca
      361 gcctaactga tatgtgcaaa gcttttggag acagcgacca gctcattaaa aactggcttc
      421 aaaataaaaa taccattgaa tttcttcaag tttgggaaga gctgaataat ccaaatttta
      481 atttggtgga attacaccaa attaaatgca acaactaatt aaaaacatcg aacagtgaag
      541 ggtaggttta taggggctta cgcccctttt gaaaactgct caaccgcttg aacgattacc
      601 tgtgtttgcg aaatgccgtg ttgttctgca agctgttcaa gttgagcaat aaaatcttcg
      661 tggaacttga atgacttcaa tcgcacacca cgctttgcat cgctttttgc ttgtaattct
      721 tgaagcgtta aacctgattt agggcgacca cgacctcgtt tctctgtttg cattgtcaat
      781 tccttgttga tttttaatac ggaacgttat attatttagg aactgcctag cagagatggc
      841 gttctctgct aggactttta gctttctaat ctaataagct ggcgtgctta ttaagattaa
      901 gatcactagg attaacaact taagatacat aatccagttc ctgatgtttg ccgctctctt
      961 caagggcggc ttcttcattt tcagaccctt tccgaaaaca agattatttt aatacatacc
     1021 aaataaaacg caagctattt tttagtattt attaaaataa ttttatttga caaccgccaa
     1081 actttaactt actattccgc ccaaggtgtc gaaacctcat actctaaggc ggatagttca
     1141 ctaatcgcca ctaggcgatt tttttatatc cgtaatcctg actatgtcgg gagggcgact
     1201 aatacaagac cgaaaggaaa tacgtccagc ccttacctta gagtgggttt tcgaacctcc
     1261 cgacgccact gtcgaaagtg gcttgtttta aacaaataga ctctaaggat tacgactatg
     1321 tcaactcaaa tttctactca aaccatttct ttcaacaacc aagccttaat cacattcgag
     1381 caaaacggca cacattacac cgcaatgaaa ccgatctgcg aaaatatcgg cttgacttgg
     1441 cacgctcaat acgaacggat caaacgagat gaaatccttt cgcagggtat tcttatcata
     1501 agaataccta caaacggtgg cgatcaacaa atgatctgcc tcccaattga atacctcaac
     1561 ggctggctct tcggcattga catcaatcgc tgcaaacccg aaatcagaga aacgctcatc
     1621 aaatacaaaa aagagtgcta ccaagccctg cacgattact ggttcaaggg caaagcggaa
     1681 cgttcacaaa tcgccccgat tgtcgaacct aaaatcccaa tggagctaac caaaaaagaa
     1741 tggctacgtt tcgcttcaat gtggtacgcc ctctacaaca gccttgaagt acttgctacg
     1801 ttagaaaaac cgttgcgtgc tatcggttcg ccgtttgggg caaccgccta cactcacgcc
     1861 accgagtatc aaaccacgct tggcgtaatg aaacggatac ttgaaccgat gttagccgac
     1921 ttcgaggtcg atccgtttga agaagctcac taccacaaag ccctaactac cttaaggcaa
     1981 taccagccga aaggcttagg cggcttagtc cgaatttaat ttgcaaaaaa atcacacaat
     2041 ccgaccgctt gtgaaacatc aagcggcggt ttcctgcacc caaaattcaa ggattaggcg
     2101 atgaaatacg caaaaatcat actcttttta accgcctttg cagttgctgc cgactactta
     2161 gagttacaca acgactgcga tggcaaacca tgcactatcg cacagcgata atgcttcccc
     2221 gaccctgccc gtgcagtgtt tatttttatc cactctcaat aggagaaacg aaatgacaga
     2281 agaagacaaa gaatatcttc aaactaaaat tgaaaacgaa ggttttgaat acgctttcgt
     2341 cagttatagt gactttgaag aagtccaaga tgaaaaattc cacgggcttc gcaaggctta
     2401 tttaaaagct cgaagtgagt tagcggaata tattgatatt gaagattaac cactgatgaa
     2461 ctgttcattg aacaaatgaa cacttgacaa ccaaccgctt gtagctaact gcaagcggtt
     2521 aatttttgga ggaaaaatgc acatagaaga caaaatcgct tggtggcttg ccaatggaga
     2581 gacaggggta agcagtaaaa caatggcttt ttaccttggt tacggaataa gaccaaaaat
     2641 agaaggctac ccacacgatg tatcggattt caggcgttgc tttctgttgt tggagacagt
     2701 cccttttttg cgaaatagga ttgaaaaaat ggctgaactt gggaaagttt gggctgcact
     2761 tgcaaaagaa tggcacacat tagaggctct ctacaacgaa gaggaagatc aaataagatg
     2821 ccccaaaacc tatgctaagt tgagagaaat cctagaagcc aacgaagaaa atgttgtccg
     2881 catcggtaat gtgagcattt cgataggttc aacgacttaa cgattattta accaaatccc
     2941 tctaaatgag gggtttattt ggagagaata tgaacatcta cactgacttc ctttctcgtg
     3001 aagagattga atttattact caatgcaagc agaaaaatct aatcaaaaaa caactcaaca
     3061 ttatgggcat tccatttaaa gaaaatgcaa atggattccc tgttgtccgc cgtgattatg
     3121 ctcaggctaa acagcgtaaa cctattgcaa cgaatgattc tgactgggta tcaaatgcat
     3181 ttaaagcgta aattgagaag aaggaggcac ttatggcacg ccctcgaaaa tatgaaaaca
     3241 acggattgcc acaaaaccta ctctgccgcc gcagaaaacg agcaaacgga agtatagttg
     3301 aatactactt ttacgttctt gccgacggca aagaaaggtc tctcggtaca aacaaacacg
     3361 aagcaatttt agaagcagcc aagctaaatt ttgaaaatag tagaaaatcc ccaattgttt
     3421 tatttattga tgtggcaaaa cgctatgaat tggaagttgt tccaaccaaa aaagccaaaa
     3481 acactcgtca atctaattta caggctattc attggctatg taaattcttt ggtaacccac
     3541 ctgcaccgct cgaaaaaatt gagccaaagc acatcagtca atatttacaa tggagaaaag
     3601 acacgccggc tgttgctaat attgaggtag gtttatttaa cacaatttgg aatatggcaa
     3661 gagaatgggg atacactgct cttccaagcc catcacaagg tgtaaaaaaa ttcccgacca
     3721 aatatcgtga agtttatgta gaggattata tccttgataa aatctatgaa tttgcagatg
     3781 aaagaatggc ggatattatc gaaacagcct atttgctggg acaaagaccg attgatattt
     3841 gtaatataca ccgctcacac atctatgacg gtatattgca tattactcaa cagaagacag
     3901 ggaaaaaagt gagatttgaa attagtggtc ggctaaaaga aattttagat aaaaggttgg
     3961 gatacgaatc agactggatt tttaccaata aatgggggaa aaaattaaaa cggcgaagtt
     4021 taggcgatca ttttaaagaa ataagggaga gagcaatgaa agcatatcct gaacttgctg
     4081 atgaaatcgc taaagtacaa atgcgtgata tgcgtgccaa agcagccacc gatatttccc
     4141 taatgacaac cgatgaacaa gctcaaaaac aacttgggca tacatcaaaa agaatgacac
     4201 atcattacat cagaaaagac aaaatcctca aacctaccga tgaaatcacc taaaaataaa
     4261 gcccttttgc caaatacaaa agagccattt actcacctaa aatccgttcc gaaactattt
     4321 tgtaaataat tgattttatt gacctaaaaa actataaaat cgcaaaaagt ttcggaacag
     4381 aaaacagcgt taaacccttg taaatcaagg attaagcacg tttgaagtct aagtgaacta
     4441 attttggttt agttgggtgg cgttgaattg cttgtacttt tacttgctct tctttacctg
     4501 caacaacgat agttaatact tcgttataga atgaatcgtg aacttgtgcg ttgttcactt
     4561 tatcgtgatc taaaatgatt gatacaggct ctgcattacc accgtagatg attgcaggaa
     4621 cttgaccatt gtgacgcagg cggcggctcg cacccttacc ttgcgcagaa cgaacttcag
     4681 cttcaaattt gaatgacata gttttattct cttaaataaa atggggaaat ccccgtgaat
     4741 agaaaaaaca gcaggcgacc cagctgtttc cgaaaaaata tcggcatatc cgtctcgata
     4801 tgccaactac gtattttact gaagttgcag aaaaatgcaa tgtaaaaata ttaagcaacc
     4861 agttttttca actgctctgc ataagcttcc caatctgtaa ttggtcgagt tgctacacct
     4921 gattgaattg ctgcttttgc tacaccaatc gagacattca cgatcaaacg tggatcaaat
     4981 ggcgttggaa taatatattc cgggccaaac gataattcgc cataagttga aataacttca
     5041 aacggcacag gttctttagc caactctgca atcgcatggg ctgctgccat tttcatttct
     5101 tcattaatgg tttttgcccc aacatctaat gctccacgga ataagaacgg gaaacaaagc
     5161 acattattca cttggttttt aaagtctgaa cgaccggtac atacaatcgc atctggacgg
     5221 acagccttcg cttcagcagg tgtaatttcc ggcacagggt tcgctaaagc aagaattaat
     5281 agatcttttg ccattgtttt aaccatttct tgcgttaaag ctccgccttt tgaacaacct
     5341 aagaaaacat ctgcattcgc aacggcatct gccaatgttc tccaaccatt atcttctatt
     5401 gcatagaatt gtttggtttc atccatctta tcaccacgac cttggaaaat aacgccctta
     5461 gaatcgcaca ttgtgatatt ttcttttttc acacctaatg aacggagtaa gttagtacac
     5521 gcaatcgctg atgctccagc accgtttacc actaaacgta cttctgaaat tgctttacct
     5581 accacttgta atccgttaat caccgccgca ccggtaataa ttgccgtacc gtgttgatca
     5641 tcgtggaaca cagggattcc catacgttca cgtaattttt gctctacata aaaacattca
     5701 ggtgctttaa tatcttccag attaatacca ccaaaagtcg gttctaacga tgcaataatc
     5761 tccactaatt tctccggatc agtttcatta atttcaatat caaacacatc aattccggca
     5821 aatttcttaa atagaacacc tttgccttcc atcacaggct tacttgctaa agcaccaata
     5881 ttgcctaagc ctaacactgc tgtaccatta gaaattactg caactaagtt acctcgtgcc
     5941 gtataacgat aagaagctga aggatctttt tcaatcgcta agcaaggctc agcaacccca
     6001 ggagagtacg ctaaagctaa atcgtgctga gtatctaatg ctttagtagg agtaacggaa
     6061 atttttccgg ggattgggaa ctcgtgaaaa tcaagcgcct gttgttttaa atcgctcatt
     6121 ttagaaacct tataatctat aaaaataaaa gtaatcaata atagccaaag agtagccatt
     6181 ttaccctaaa ttttttcgca tttttatggc tttgctctca ttttttatga tatttacaaa
     6241 aaataataca aaaacgtccg cttgttttac tcaaatcaag cattcttcat cttattgata
     6301 aaataagaaa ccatttcaga agctatatca acatcattta ctctctctat gccttcaaat
     6361 cctgggctgg cattgacttc taaaataact acgcctttct ctgtccgcag aaaatcaacg
     6421 cctgcaatat caagccctat cgttttagtt gctttaattg ctaacgcacg ttccatctga
     6481 gttaattcaa tcggttgagc cgttccacct tgatgaatat tggctctaaa atcgcctgtt
     6541 acgctggttc ggctcatggc agcaaccact tcattgccaa tcacaaacgc ccgaatatct
     6601 tgccctttag cgtcgcccac aaattgttgg catagataag gctcatttac ttgcctaaag
     6661 gtcgctaaca cggcctcggc attatttttg ccttcaaata gcattacgcc attcccctgt
     6721 gaaccattta gcacttttga aataagtgga aaagcaaatt gattgagttg tgatttcaca
     6781 gaaaccaaat gccctgcaaa attcgtatta ggaaccggca aattgtgttt ggctaatttt
     6841 tgtaatgttc gccatttgtc tcgggctaac gcaaatgccg ctgaatgatt tagaacagga
     6901 atacctttcg cctcaaaatg gcgtaacacc gaacagccca tttcggtact gcctgtgcca
     6961 aaacggggta aaatgccgtc ataattcggc aacaataccg gctccggacg gttgttatca
     7021 tagctctcgc cagcttgata gtagatctta aattcgccat tttctaaccc gagtagcatt
     7081 ctattctggt ctaaaatatc cagctcaatc ccctttgcct cacaagcggc ttttattcga
     7141 cagcagctat ataaacgagg ctctcgacat agcattagcc acttcatttt ttcagcaatc
     7201 ctttctgatt gaaaaacggc aaaatatgcg gacgaacagg cttatccaat gttttatgaa
     7261 tagctgcttg ccattgttgt ggctcgccga ttctggcttg gtagtaagcg gtcaaatttt
     7321 cattatattt tgcaaattca gtccaatcaa aatcctgata ttgattgtta taacaaacca
     7381 aactattcgg caaacgaggt tttattgccg gctcttgtgc cggatacccc aaacacatac
     7441 ccaccagtgg aacacaatat tccggcaagt taagggcttt agaaacccgc tcaatatcat
     7501 tacgcaatgc tccaatatac accgctccca gccctagtga ttcggcggct aaaaatacat
     7561 tctgggccaa aataccggta tctaccgccc caatcaacga cacctccgcc cattccaact
     7621 gcgattcagg gacaagttgt ttgtgtttat taaaatcaat gcagaacacc aaaaattcag
     7681 cacaatctac tacatattgc atacctgaaa cggcttttaa tgtttctcga atctgccgat
     7741 cggttacacg aatcaccgtc acacattgca gatgatttga acttgaagcc gctttacctg
     7801 cctcaattaa gatttggaaa atctcttcag aaataggctg ttcggtaaat ttacgaatag
     7861 aacggtgaga taaaatcgtc tcaagagtcg gtttactttg catatttttc tcgtctaaag
     7921 tttatctaaa taatttttgc catcaagcaa acttacttgt cgcataatcc acgcttggcg
     7981 ttttcgcata ctcgggccgg gtttatctac cctaaacacc aacgggttag gcaaagaggc
     8041 tgccaatagt gcggattctt gcaaggttag ctgtttggct gattttttga aaaaatgctt
     8101 tgcggctgct tccaccccga aaatgccgtt accaaattcc gcaatattga ggtacacctc
     8161 taaaatccga tccttactcc acacattttc gactaacagg gttaaaggca cttcaatccc
     8221 tttgcgtaac cagctttgcc cgtgccacaa aaacatattt tttaccgttt gttgcgaaat
     8281 ggttgaacct ccacgcactt ttttggattt agcattcagt ttcaaggcag tttctatcgc
     8341 ttgtaagtca atgccaaaat ggctttcaaa cttttgatct tctgaggcaa tcacagccat
     8401 ttgcatttgc cacgaaatct cattcaggct cacccattct tttttgatcg ggtaagattt
     8461 cccctgaacc agattctcca cctttttttg caccatatac gcagaataag gcaccggtaa
     8521 caccgaaaaa atcagtatcg gaatcaggaa aaatgtgcca ccaaaggtta ataagcgact
     8581 agaaccaaac caaagccagc ccagtttact ttttattttg cgaggaataa aaaaaagccc
     8641 tattttcttc aaaagagagc ttaaaaatcg cttgttacgc tttgccattg actgttccat
     8701 taaccttgcc gttcagccat tgcagaaaat cttggtcgag tgttttaagc atatttgaac
     8761 cacgagtgat ggttgccgca ctggtattta aattttgttg gatttcccgt tgcgaatctt
     8821 caccttttag caaagcttgc acaatttgta aacgcaagcc taatgaacct cgctcatcgg
     8881 cggtgagaaa cattgagaaa aacggctcaa gtttatcttc cgcaaccgct tgttcaagca
     8941 gagccaaaaa ctgcgtccac tcgtttggat ctcgctggtt atagagtgtt ttcatactgt
     9001 cccttgaagt aaaaacggtg acaagcggtc agatttttac aaaattttgc agaaaaacga
     9061 ccgcttgctc tccattattt tttcgatttt ttaccgccac gttggtcgtc taactctgcc
     9121 cctttcggta cgctgaattt aaacaaatcg cttgagacac cgccggtagt aatattgcgt
     9181 aacacatata agttactttg cccgtcacgc tcaatggtac tgaaaccttt taataaaccg
     9241 ctttggtcaa tccgcacatc aaactgtttt aaattgcttt ttttcgattt tggcttaagc
     9301 acgaaagtat cagcactctg gctgacatca tattgatccc agtggctttt atcaccgctg
     9361 gttaataaga cgaatggcgt attattaact gcatcttgca cggtatttac cgtaacttgt
     9421 gaaacgaaag gatcgtaaaa ccataaatct ttgccgtcag atacaatcaa attttcttgt
     9481 ggtgatttgg tatccattct aaataagttc gggcgtttta cttggaattt gcctttgcct
     9541 tgctgaattt gtttaccttt gctagaacgc acggtttggt cgaaatccgc actatattgg
     9601 cttacttgtt ccaaacggcg ttgtaattct gcaacagatt gagcatcagc caatgccaca
     9661 tttgccaagc caaataacgc taaaccgaat accgattttt taaataatgt tttcatttgt
     9721 tttccttttt accaacaaga attttcttgt ttgacaataa aatcatcaaa agtttcatat
     9781 taatgaactg ttacaacaat acaataaaag tcgtgaattg tatagtttaa tgagcttcgt
     9841 cccagttttc gcctacgcca acttcggcaa tcagtggcac tttgaggcta atcgcttttt
     9901 ccatttcggt tttgattaat tggctgtaat ggtcgatttt atcggctttg acttcaaaca
     9961 ccaattcatc gtgaacctgc ataatcattt tgatttcatc attatcacga atcgcattat
    10021 caataccaat catcgccacc ttaataatat cggcagccgt gccttgcatt ggggcgttga
    10081 ttgccacccg ttctgctgct ttacgcaaaa tagcattgcc ggatttaata tcaggcaagt
    10141 acaaacggcg accaaatagg gtttcaacat agcctttttc aactgcaact tcacgaatat
    10201 cggtcataaa ttgttgaacc gccgggtaac gttggaagta gagatccata tatttttgag
    10261 cctctgcacg cccaatccct aattggtttg aaagcccaaa tgacgacatt ccgtaaatca
    10321 gcccgaagtt aattgctttc gcactgcggc gttgttcgga ggtgacttca cctaactcca
    10381 ccccgaaaat ttcggcagcg gtagcacggt gaatatcttt gccctccgca aaggcgttaa
    10441 tcattccctc atcattcgat aaatgagcca taatccgtag ctcaatttgt gaatagtcgg
    10501 cagcgacaat tttataacct tctctcgcga caaaagcttg gcgaatccgt ctgccctctt
    10561 cattgcggat cggaatgttc tgcaaattcg gatcgctgga agaaaggcgt ccggttgcgg
    10621 tgactgcttg attgtaagag gtatgcaccc gccctgtttt tttgttaatc agttgtggta
    10681 atttatcggt gtaggtagat ttcaacttac ttaacccacg atgctccatt aataacttcg
    10741 gcactaaatg cccttgttga gccaactcgt ctaacacttc ttcgttggtt gagggtgcac
    10801 cttttggagt tttcttcaac accggcaagc ctaatttttc aaacaaaatc tcttgtaatt
    10861 gtttagttga ggcaagattg aaggtttgcc ctgcttctgc gtgtactaat gtttcaactt
    10921 cctccaaacg tttttcaatt tcagccgatt gggtcaataa ttttttcggg tcaatcaaca
    10981 cgccattgcg ttcaattcga gaaagtacgc ccacaagtgg catttcaatt tcatcgaata
    11041 acttcaccaa ttcaggctct tgctgtagtt tttcccacaa tacttggtgg agtttcatcg
    11101 tcacatcggc atcttctgcc gcatattcag ccgcttttgc tatctcgatt ttatcgaagg
    11161 tcagttgatt tttcccctta ccagcaagct cttcaaacgg aatcgtttta tgccccaaat
    11221 aacgggcggc taaatcgtcc atattatgac gaccggtgct gttttgggtg taagattcaa
    11281 tcatcgtatc aaaagcaaca ccttgtagct caatgccgta gttcgcaaaa atggttaaat
    11341 catatttaat attctgcccg atctttttaa tctccgggtt aagcaaaatc ggcttgagtt
    11401 tacttaaaca agcggtcaaa tttagctgat tttttaccaa ttcatcggaa gacgggcttt
    11461 ctgaacgctc ctcatcaccg aacatatcgg cttgttgcgg ttgggctttt tgcttatgcc
    11521 caagcggaat atagcacgcc tcgccatttt ccagtgcaaa ggaaataccg accaaattag
    11581 ccgacattgc gtcaaggctg tcggtttcgg tatccaccgc aatcaaacga gctgcggtga
    11641 gtttttcgat ccaggctgat aatttctgtt cagtatcaac ggtctcatat ttgctacgat
    11701 cgatctcgac cacatttgca acattttcct catttctgac cgcttgtgtt acttggtaat
    11761 tattcggtac tttttcagcc gaagtttgag tgaccggatt ggcattttga ttcacttcgt
    11821 tcagccagcg tttaaattca taatgctcaa ataattgagc aagctcagca cggtcttgcg
    11881 gctgtgttaa aagttgatcg tgagtaacat caagttccac atcggttttg atggtagcaa
    11941 gcaaatagga taaatcggca ttcgcttttt ccgcttccaa ttttggggcg aaattttttg
    12001 ccccacggaa agaaagggtg gcaatttgat ccaaattagc ataaatctgt tcaagtgaac
    12061 caatgccttg caataaagct aatgcggtct tttcccccac accttttacg cccggaatgt
    12121 tatcggcgga atcgcccatt aatgccagaa agtcgataat caactcaggc ggaatgccat
    12181 atttttcaat cacgccttca cgatctaaca aagtattgtt catcgtgtta atcaacataa
    12241 tgtgatcgtt caccaattgt gccatatctt tatcgccggt gctgatcaac acatctttgc
    12301 cgtctttagc ggcttgcaca gcaagcgtgc cgatcacatc atcagcttcc acaccttcaa
    12361 tcgaaatcag tggaatgcct aaggctttga taatgcgatg caaaggctca atttgagggc
    12421 gtaaatcatc gggcattggt gggcggtgcg atttatattg cttaaacaac tcatcacgga
    12481 aggttttccc tttggcatca aacaccacag caatatggct tggattcact tgtgaaatca
    12541 ggctttttaa catattcagc acgccataca ttgcccctgt cggttcgcct aagcgattag
    12601 tgagcggagg gaaagcgtga aatgctcgat ataaatagga agaaccatcc accaatacca
    12661 gtggattttt tgcgatagtt gccataaaaa tctctttata atcaaataaa tattggcgtt
    12721 attatacaag aatattt
//
LOCUS       DANLUA010000066        12507 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLUA010000066 DANLUA010000000
VERSION     DANLUA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(313..495)
                     /locus_tag="PXB44_001283"
     CDS             complement(313..495)
                     /locus_tag="PXB44_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281761.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            645..1685
                     /gene="selD"
                     /locus_tag="PXB44_001284"
     CDS             645..1685
                     /gene="selD"
                     /locus_tag="PXB44_001284"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:690..692,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL5281762.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1685..2158
                     /locus_tag="PXB44_001285"
     CDS             1685..2158
                     /locus_tag="PXB44_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL5281763.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2148..3356
                     /gene="ubiH"
                     /locus_tag="PXB44_001286"
                     /gene_synonym="visB"
     CDS             2148..3356
                     /gene="ubiH"
                     /locus_tag="PXB44_001286"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL5281764.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3619..4851
                     /locus_tag="PXB44_001287"
     CDS             3619..4851
                     /locus_tag="PXB44_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL5281765.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4918..5904
                     /locus_tag="PXB44_001288"
     CDS             4918..5904
                     /locus_tag="PXB44_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5281766.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5945..6355)
                     /locus_tag="PXB44_001289"
     CDS             complement(5945..6355)
                     /locus_tag="PXB44_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL5281767.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXB44_001290"
     CDS             complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXB44_001290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL5281768.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXB44_001291"
     CDS             complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXB44_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL5281769.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXB44_001292"
     CDS             complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXB44_001292"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL5281770.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10256..10987)
                     /locus_tag="PXB44_001293"
     CDS             complement(10256..10987)
                     /locus_tag="PXB44_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL5281771.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXB44_001294"
     CDS             complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXB44_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL5281772.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXB44_001295"
     CDS             complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXB44_001295"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL5281773.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
BASE COUNT         3498 a         2543 c         2795 g         3671 t
ORIGIN      
        1 aggaaaaatg tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg
       61 ggtggaaaag aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggagac
      121 tgtcagatca agtctgatct tctacaacta agccttctca gaataaataa attttcacaa
      181 gcggtgcaac tttgcaaaaa cttcggtatt atgtagcagt tgcacaaaac cccaaattca
      241 ttaaaaccgc ttaaaaatga aatgccctaa actgattttt cagtctaggg catttattat
      301 cacatattat ggttaatagg gaaaactcac atcattgtta tgctggtgat atttttgcca
      361 aaatgcgtga tattgttttt tcaccgatgt gtttaataaa aacgggatta tcaagacata
      421 ggtcgggcaa ttccatagca ttgcacgttg tgtatccgtg ccgattttgg tgcgtaagta
      481 accaaactga tacattagac ccaatccaac cgtcacctat tctaatagca taataactaa
      541 ggtcgcatca ttaaattaaa agctcatttt ttgcaaaaaa tttcccaaat tccaccgctt
      601 gttggcgtac aataggcata attttgattt acgatggagg cagaatgtct gaaccgataa
      661 aacttacgca gttcagccac ggggcgggtt gaggctgtaa aatttcgcct aaggtgttag
      721 ggacaatttt acaaactcaa ttagaacaat ttgtcgatcc gaatttattg gtaggcaatg
      781 aaacggcgga cgatgctgcc gtctatgatt tagggaacgg gcaggccatt atcagcacca
      841 ccgatttctt tatgccgatt gtggacgccc cgtttgattt cgggcgaatt gctgctgcca
      901 atgcgattag cgatatttat gcgatgggtg gcaagccgat tatggcgatt gcgattctgg
      961 gctttccggt gaataaattg ccggctgaag tggctcaaca aattgtcgaa ggtgggcgtt
     1021 ttgcgtgcca gcaagccggt attgccttgg cgggcgggca ttcgattgat tcgcccgatc
     1081 cgattttcgg cttggctgtt accggcattg tgccgaccga acgggtgaaa cgcaatgcct
     1141 ctgcggtggc tgggtgtgag ctgtttttaa ccaagccttt ggggattggc attctaacca
     1201 cggcagagaa aaaaggtttg ttgaagcctg aacatcaaca tctggcaagc gaggtaatgt
     1261 gccaaatcaa cttgattggt tcagcgtttt ccgaactgcc tgaagtcacg gcaatgactg
     1321 atgtaaccgg ttttggctta ctcgggcatt taagcgaggt gtgtcaaggc tcgggcttac
     1381 gggcggaggt tgattttgag gatattcaag tccttgatgg cactaaggag tatattgcat
     1441 tgggagcagt tccgggtggg acaaatcgca atttcgacag ctatgggcat ttgatttcgg
     1501 caatgacgga cgagcaaaaa gcgattttgt gcgatccgca aacctctggc ggtttattag
     1561 tggcggtgct gccacaagcg gtcgaaaaag tgcaacaaat tgcaaatcgg gcaaatgtgc
     1621 cgttattcca cgttggcaga ttgctggaaa tggaacaggg caaaccacgg attgaggtaa
     1681 tttgatgttt aaaccaaatg taacaatggc ttgcgtggtg cattgtaagg gaaagtttct
     1741 gtttgtggaa gaaattgaat atggcaaacg tacccttaac cagccggcag ggcatttaga
     1801 aaaagatgaa accttgctgg agggggcaag ccgtgagctg tttgaggaaa ccggtattcg
     1861 ggcagaaatg gcaaatttgg tgaaaattta tcaatggcaa gcccctcgtt cacaaaccga
     1921 tttcttgcgt tttctgtttt cggtggaact ggaagagtgg ctggagccga atccgcaaga
     1981 tgcggatatt acccaagccc tttggcttac tttagcagaa tttgaagact acattcagca
     2041 acaagggcaa tgtgccagaa gcccgttggt ggttcaatcg gtcaaagatt atttaagcgg
     2101 tgagcgttat ccattaagtt tgttgaatgc gtttgaatgg gcgagaaatg gaaaataaaa
     2161 caacaaccca atttgatgtg gtgattgtgg gcggggcagt gaccggctcg gtgctggcat
     2221 tggcgttaag tagttttact catcacaaaa tctcgattgc gatagtggaa aaatcgctgc
     2281 cggactatga acagcaaggc ggttttgatg ctcgcagtat tgccttagcc gaaggcagtt
     2341 tgcaaaaaat ggggcaaatc agaccgcttg caggctcaag tttaggcgag atgattagcg
     2401 aaatcggcac gccgattcag caaattcaag tagcggataa agggcatttt ggtaaaacga
     2461 ccttgaaagc aagcgagcag aatgtggctc aactgggtat ggtggtggaa cttgcaaaat
     2521 tcggcagaaa attgaccgct tgtcttgcac agcaagctaa tattacgctt ttttgcccga
     2581 atcaagtggt tggcattgag cgttcccaac aacattgtgg attgagcttg aaaaatggtg
     2641 agaaactaag ctgttccttg ctggtggcgg cagatggtat tcaatctcag attgcaaaac
     2701 agtgcggtgt ggaaaccgta aaagtaaggg attatgcaca atcggcaatt atcgccaatg
     2761 tggaactgag tgaatctcac caaaatcaag cctttgaata ttttacgcct caaggccctt
     2821 ttgctttatt gcctttgagc ggaaatatga tgtcgttggt gtggtgtgtg aaagagccga
     2881 acgagttaat ccaactttca gacagtgaat ttttagcccg tttgcaacaa caatttggtt
     2941 ggaagttagg gcaatttgtt cgagtgagca agcggtttgt ttatccgctc attttgcaaa
     3001 aagcccagtc gcatattcac caccgcttag cagttgtcgg caatgccgca caaatgcttc
     3061 acccggttgc tgggcaaggt ttcaacttag gcttgcgtga tttgactacc ttagcggaat
     3121 tagtggcttt ggcgtttaat caagggaaag atataggcga attttcgctc ttatcaactt
     3181 ttgagaatga gagaacgcaa gatcaagcca gaatgataaa tgctacaagc ggtctgattt
     3241 cgcttttttg ttgcgaaatg ttaccagttc aggttgtgag gaatttaggg ttattaacgg
     3301 tttcccattc aaaaatagcc agagattttg ttgctaatca ggcattaggt tggtaaaaaa
     3361 cacaaaagca gcaattttct atttgacact gtttttaaaa aggcgtatta tccaacccgt
     3421 ttaaaaatct caccaaagcc ttgaatgcaa attcaaggct ttttcctttt tagtgaaagc
     3481 ctatgaattt tgtttgccgt aaaatcggca aaaaaatttt gcctaaaatt cgcaatcgtt
     3541 tgcgaaatag gtttatagcg ttgttttcat ctgggtaact gcaatggtta catcgcaata
     3601 catcaaagag agtaaaaaat gaccacagaa aatatgagta cgccaaagca ggcgtttatc
     3661 gggctgcaaa tgctgtttgt tgcatttggg gcgttagtat tagttccgct gattaccggt
     3721 ttagatccta atgttgccct tcttaccgcc ggtgtcggta cgttaatctt ccaaatttgt
     3781 actaaacgtc aagttcctgt tttcctcgca tcgagtttcg cttttatcgc tccaatccaa
     3841 ttcggtgttc aacaatgggg aattcccgtc acaatgggag cattgctctg ttccggtttt
     3901 atttatatgg cattatctgc tttagtgaaa gcgaaagggg tgaatattct gcataaatac
     3961 ttccctccaa tcgtgattgg accggtaatt atggtgattg gtttaggtct tgctcctgtt
     4021 gcggtggata tggcaacggg taaatcggcg aatattgcct ataacaccgc tattattgtt
     4081 tcaatgataa cgctggtaac tacgctgatt gtggcggtgt ttgcgaaagg gctattaaag
     4141 ctcgtgccaa tattagcagg tttaacggtg ggatatatct tgtctgtttt tatgggattg
     4201 gtggatttta ctaaagtggt tgaagctcct tggtttagtc taccgaaagt ttccgcaccg
     4261 gaattcaaat tagatgcaat cttgtattta ttacctatcg cacttgctcc ggcagtcgaa
     4321 cacttaggcg atgtaatggc aatcagccaa gttgccggta aagattttat gaaaaaaccg
     4381 gggttacacc gcacgctttt aggtgatggt ttagcaactt cagcagctgc ggcattaggt
     4441 ggcccaccaa atacgactta ctctgaagta accggtgcgg taatgctaac caaaaacttc
     4501 aatccgaaaa ttatgacttg ggctgcgtgc tgggctatcg ctttctcatt ctgcggaaaa
     4561 atcggggcat tccttgccac tatccctact tttgtgatgg gcggtattat gatgttgctg
     4621 ttcggtgcag ttgcggttgt ggggatcaat actctaatta aagctaaagt tgatttaagc
     4681 attccacgta atctttgtat tgcttctgta gtaatgacat tcggtattgg cggtatgtta
     4741 attaacatcg gcgaattctc attaaaaggg atcagcttgt gtgcattagt cgctattgta
     4801 cttaacattg tattaccaaa agaaaaagta gaagattctg ctgctcatta atcaaaggac
     4861 gctgtggcgt cctttttgct aataaccctc tatttgtaaa taaaaaggaa taacactatg
     4921 caaaaactca cattttcacc accactttta aatacgcctt gcccttggtg ttcggagtta
     4981 gagagcttgc gtgaacttta tgcgtgtgaa tataccggtg cggtaacaac ccgtacttca
     5041 atgcctcaac catatccaca cgattgggcg aagaaccaat atgtactctt tgattccaac
     5101 gcacagaaaa cagcaagtgt gaatactcaa gatgcaacta gcttacaaac agcaagcctt
     5161 aacaccatcg gtttaagccc aaataatctg gataccacca tcagctttgt acgcaccatt
     5221 tcaaatgagc tgacagcgcc atcaagcaaa ccatttatta tttcggtgtt tggttcaccc
     5281 gaagaggtgg gggaatgtta tgaaaaaatt gttgctttcc aaactgaagt gaaaatgcct
     5341 ttggcaatgg agattaatat ctcttgcccg aatattccgg gtgaaatttc gcctgcttat
     5401 tccgcagaag aattatcaca ttacttgcac gcactacaaa cttcgctaaa aaaactggct
     5461 caaggtgata atactttacc aatcgggatt aaaattccac cttttactta ccaaaatcaa
     5521 tatgatgaat taatcaaagg cttattacaa gcggtcgaaa atggcgaaaa tttaccaatt
     5581 tcctttatta cttcaaccaa tacgcttggc tcatcgcttg tcttaaatgc tggcaaagcg
     5641 gtattaaatt cagaagtggg aacgggcgta ggcggaatgg caggaacggc aatccatgca
     5701 ctttctttgg ggaatgttta cacgattcgc caaatgttag atcaagagcc taagctcaaa
     5761 catcttcaaa ttattggtgt gggcggcgtg aatgataaag ccggctttga gcgaatgaaa
     5821 tcggttgggg cagactttgt ggggttagct accgcattag gggtgaaagg aatcggcgtt
     5881 tttaaagaga ttctgtctga ataatgaaga ggagggcgtg cattgcacgc ccgaataaat
     5941 gaaattagcc taaccatttc tccactaatt cattagtggt ttctttatgt acccacatac
     6001 tgccaagttc cggcattggg aatttggtgt ggttgtagcc gacaaattgg cggaaatcaa
     6061 acacggcgtg cgggtagccg ttgatcagta aaaaggcttg atagccgtct tcactccagc
     6121 aaatttctaa acggcgaggt tcttttaatg aattttgctc aatatcgctg acccgaaaca
     6181 caaataaact atccactacc ggctgttcgg tttggtgtaa gtccattgca taaaagtagc
     6241 cgctttcttc atcaatttca aacatcacta ctaaatgttc gtgctgggtt gaatgagcgc
     6301 cattacggta aggttcgttc agtagaatat gagcagttaa aacagagtag agcattaaat
     6361 tatccttata agtaaaatgc caccgatttc tcagtggcat ttaaatattt aatcaggcaa
     6421 ttacgcttga cctttaatct ctttacggcc gttgaacgga gccggtgtac cagcacgctc
     6481 taacgcttct tcgatacgga ttaactggtt gtatttcgct acacggtctg aacggctcat
     6541 tgaaccggtt ttgatttgac ctgctgctgt acctactgct aaatctgcaa tggttgcatc
     6601 ttcagtttcg cctgaacggt gagagataac tgcggtgtaa cctgcatctt tcgccatttt
     6661 gattgcagct aaagtttcag ttaaagaacc gatttggttg aatttgatta agattgagtt
     6721 tgcgatacct ttctcaatac cgtttttcaa gattttggta ttggttacga ataagtcatc
     6781 accaactaat tgtactttgt cgcctaatac tttagtttgg tatgcgaaac cttcccagtc
     6841 agattcgtct tgaccatctt cgatagacac gattgggtac tctttgcata agccttctaa
     6901 gtagtgagtg aactcttgtg aagtaaaggt tttgccttcg cctttcatat cgtacacatt
     6961 gcgttctttg tcgtagaatt cagatgatgc acagtccatc gctaaagtta cgtctttacc
     7021 taacacatag cctgcttttt ctaccgcttc tttaatgcac gctaatgcgt ctgcgtttga
     7081 agccaagttt ggtgcgaaac caccttcgtc acctacagca gtatttaagc ctttcgcttt
     7141 taatactttt gcaagattgt ggaatacttc agcaccgata cgaagagctt cttttaatgt
     7201 agaagcacct accggttgaa tcataaattc ttgaatgtcc acgttgttgt cagcgtgttc
     7261 accaccattg atgatgttca tcattggtaa tggcattgag taaacacccg gtgtgccgtt
     7321 taattcagca atgtatgcgt aaagcggtaa gccttttgaa gcggctgctg cttttgctgt
     7381 tgctaaagaa accgctaaga tagcgtttgc accgaatttt gatttgtttt ctgtgccatc
     7441 taaatcgatc ataatttggt cgatttcagc ttgtgcagaa ccgtctttgc ctactaatgc
     7501 gtttgcaatt tcgttattta cggcagaaac cgcttttaat acacctttac ctaagaaacg
     7561 acctttgtcg ccatcacgta attctaatgc ttcacgtgaa ccggtagaag cacctgatgg
     7621 agcggctgct aagcctacaa aaccaccttc taagtgaact tctgcttcta cagttgggtt
     7681 accacgagag tcgatgattt cacgaccgat tactttaaca attttagcca ttgttgatgt
     7741 cctctaatat acgagttaat agaaaaaaga ataggtatat agtaaagcga ttttctgctt
     7801 ttgtcttgga aaaactgatt gagtggcaga aaatttgcca ctaatttgat ctaagactat
     7861 attcgcctga gattatagca acctttgcaa aaaacggcaa aaatttgccc gcttgtaatg
     7921 cttatttttt gactaaaaaa gtcaaggttt cataatgtgc tgtgtgagca aacatatcaa
     7981 acagctgcac tttgtgtagt tcgtaatttg tcaaaaatgt aaaatcgttc gccattgaga
     8041 cggcattaca actggagtaa atcaaataag gtgagcctaa ttgatttaaa aattctgcta
     8101 atagtttgcc gataccacgt cgaggtgggt tcacaatcac taaatcgggt ggagaaccag
     8161 cttcatttaa agcaaattgt gcagaatcta atgaggcaaa ttttacctgc tttaggccga
     8221 gtttttcagc cgatttggtc gcacttgcaa tagctgaggc tgaaatctca atgcctgtca
     8281 gctccacgtt cgagttcttc gcttgcaagg cgttggcaca atgtaagccg aaaccaccga
     8341 caccgcagaa caagtcccac aatttagcaa taggtaaatg ctcaatccag ctttgtgctg
     8401 tgtggtaaag ctggcttgct accacagggt tagtttggaa aaagccctga gggcgaataa
     8461 atagcggtat gccgttaaaa tgttcttcca gcactttttg ttcggtgagg aaaatctcag
     8521 tttcgccttc taaaagagca gcgtgttggg gctgaatatt cagactaact actgagttgg
     8581 gtggcagttt ttccaacagt tcgggcaatt cccgttcaac caatgcacgc tttgcttcac
     8641 tacgaaccac aaaacggagc ataaatgatt gattatattg gctttcggta atcagaatat
     8701 attttagctc accttttttc ttactgatgt tatacggcac taaaccagct cgagcgataa
     8761 aatctttcaa aatcggaaaa agtctatgaa atgaggacgg gtagagcaga cagtcggtta
     8821 aatccacccc gctttgagca tcattttgat cttttaaaat gcctaaaatc gggcgttcta
     8881 cactgccgga gaccaccatt ttcgctttgt tacgaaattg cgaaattggc gagccgacaa
     8941 gcggtaagat ttccgtttta ttttgcaaaa taaacggcaa aatcaaccgc ttgagatcgg
     9001 cctctttggc ggctaattgc gtgctgtatg gcttttcaat ccactggcac gaagtacagt
     9061 cgccacgctc aaaatgcgaa caatgcaaca agctagacat tcgcttcctt ccattccagc
     9121 cagttgtttt gtaaggccgt taattcagcc tgagtttgtt ggtagcgaga gctgttttcc
     9181 agctctcgcc aagtcacggt tttgcgtttc attaaagaaa ggcgacgttc tttttgtgct
     9241 tgagtcggct gatatttcag cccgtctaac acttgcacca ggccttcagg ctcgttacaa
     9301 aggagcaata aatcacagcc ggcttggatt gctttttcag aacgctcgac aaaattgccc
     9361 ataaagcccg cacctttcat tcccaaatcg tcagagaata tcacaccgtt aaagttcagc
     9421 tgtttacgca atacctcttt aagccaatag cttgaaccgc ttgccggctg gctatcacat
     9481 tgggtgtaaa cgacgtgggc aggcataatg gcagagagtt ttcctttggc aataagctgt
     9541 ttgaacggca gaatatcgtg attaaaaatc cgctctttcg ggcgatcatc aaacggggtt
     9601 tctaaatgcg aatcggctaa aacgtgtcca tgaccgggga agtgtttgcc ggtggtcgcc
     9661 ataccgattt cacgcattcc gtcaataaag gcttcggcaa caggcaaaat cgctgctggc
     9721 tcttgcccga atgagcgatc gccaatcgct ttgcattcgt gtcctaaatc cagtaccgga
     9781 gcgaaactga ggtctatatc gagggcaaac atttctgctg ccatcaacca gccggtttct
     9841 ttcgctaatt gcattgcttg ctctggcgtt gtggcaagtt ggttaaaaga ttgcattgcc
     9901 ggcagtttag taaagccttc acgaaagcgt tgcacccgac cgccttcttg gtcgacagta
     9961 ataagcaacg gttttttcac ccgctggcga atggatttca ctaatgcctg aatttgtgct
    10021 ttgtcgtaga aattacgggt gaataaaatt agcccggaaa ctaaagggtg ttccagcatt
    10081 tcgacttctt cttgagtcag ttctttatct ttaatatcaa tcagtaacat aattttcgtt
    10141 gagctatttg caaaaaaatg agcagatatg accgcttgtt gtgcctattc tacattaaaa
    10201 agcccttgat gttgatcaag ggcgaatgat taagggcata aatgagacag agcaattatg
    10261 cttgttctaa tttagcttgg ttacgacgca tttttatgtt gagcatttca accacaaccg
    10321 agaagcccat tgccgtataa accgctgctt ttggaatatg aatatcaaaa ccttcgccga
    10381 ctagtacaat accgattaaa atcaagaacg ctaaagcgag atttttaatg gttgggttgt
    10441 tatctacaaa gtccccaatg gttttcgctg ctaacatcat taccgcaact gcaatgataa
    10501 tcgcaataac cattaccgga atatcatctg ccattgccac agcagtaatg actgaatcaa
    10561 gggagaaaat aatatcaaaa attgcaattt gaattaacac cataaaaaag ctggcttttt
    10621 taatggattc gcttttttct tcgtgagatt cggcagaaat ggattctttc agctccattg
    10681 cacttttaac aatcaggaat aatccaccga tgagtagaat gagatctcta cctgaaattg
    10741 gattgttgcc aatagtgaaa agaggctcga ccaaacgcat catccaagca agcgagagaa
    10801 gcaataaaat acgggttccc attgccagcc ccaagccgat aattcgggca gattggcgtt
    10861 gttcaatagg taaacgggaa acgagaatac tgatgacgat gatgttatca atgcctaata
    10921 cgatttcaag cccggtaagt gtaaagagcg caacccaagc ttcaggattt gtgatccagt
    10981 caaacataaa aatgtccttt taaaaataag ggattaagtg atattttcta tcaaatagaa
    11041 ttaaagccga catcttatag agtgtgagga caaatttaaa gggggcaatg caaaaagttt
    11101 tgtaaaattt cggcggtaaa atgtttacgc aaatagaaac caagagggag agattgaaca
    11161 atctcccctt tagcgttgat agcagaggtg atagagcggt tatttctacc tttgggtctg
    11221 agttgtaaca cttcgcctaa ggttgcatta attttatgca gttcacctaa cacaatcaac
    11281 tccattaatt cctcccaatc ttgctgtagg cgggattctt gtgtgatagt cggagaccat
    11341 aaaatcggct gaccgatatg gcgttcggca aggggtatct ttcgttcccc ttcaaccgga
    11401 atccaaagca cacgggaaag cttatgttta acgtgggagg tttcccaaat aataccgttg
    11461 ttttgggtta aaggagcaag gctgacgaat gtggtttcca gcggtaatcc tttctgattg
    11521 accggaatgg ttttcagctc aatgcctaaa tgtgcaaaat cctgttcggc tttactgccg
    11581 gctttcgcac ctaaagcggt ttcaatcaga gtaccgaccc agcctttatc tcgtttcaga
    11641 tctgctggta tcggaatatg aagcatctcg gcaatttcac ctaaagtgaa ccctgccagc
    11701 cattccgctt tgcttaataa tgcctgctcg gttggagaga atgtagataa ttgcattgca
    11761 cttagttaat tagactaaac tggcaagcgg tcaaatttag gcagaaattt gcaaaaaatt
    11821 actaaaaaat aaccgcttgt gaggaaatta catccaagca ttactgcgaa taacgcctac
    11881 tgcaatgcct tcaatttcaa tatattcttg agcagggttt actacaatcg gttttaattc
    11941 atcgttttca gggtgtaagt aaattaattc acctttcttc tctaagcgtt taacagtcac
    12001 ttcatcattt acccgagcaa ccaccacttg cccgttacga gcggtattgg ttttatgaac
    12061 ggctaatagg tcaccgtcta aaataccgat tttttccatt gaattgccat ttactttgag
    12121 taaataatca gcacttggat taaacatcgc accattcacc gggtaatggt tttcaacgtg
    12181 ttcaaccgct aaaataggtg tgccagcagc tactttgcca atcaacggta agccttcatc
    12241 ttcatcgttg gcagcttggt tttcgtcttc gactaaaatg cgaattccac gagatgtacc
    12301 tgataacatt tcaatatagc ctttacgagc aagggctttt aagtgttctt ctgccgcatt
    12361 aggtgattta aagccgattt cacgggctat ttctactcgg gttggtggca taccggtggt
    12421 ttcaatatgg tttttgacga aatcaaaaat ttcttgctga cgggcagtta gatgtttacg
    12481 cattttaagt taatcctcac tgtcttt
//
LOCUS       DANLUA010000067        12395 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLUA010000067 DANLUA010000000
VERSION     DANLUA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12395
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            139..798
                     /locus_tag="PXB44_001296"
     CDS             139..798
                     /locus_tag="PXB44_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5281774.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(858..2183)
                     /locus_tag="PXB44_001297"
     CDS             complement(858..2183)
                     /locus_tag="PXB44_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL5281775.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(2284..3036)
                     /locus_tag="PXB44_001298"
     CDS             complement(2284..3036)
                     /locus_tag="PXB44_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL5281776.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            complement(3036..3941)
                     /locus_tag="PXB44_001299"
     CDS             complement(3036..3941)
                     /locus_tag="PXB44_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL5281777.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            complement(3950..4513)
                     /locus_tag="PXB44_001300"
     CDS             complement(3950..4513)
                     /locus_tag="PXB44_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL5281778.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            complement(4513..5289)
                     /gene="yaaA"
                     /locus_tag="PXB44_001301"
     CDS             complement(4513..5289)
                     /gene="yaaA"
                     /locus_tag="PXB44_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL5281779.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            complement(5478..7198)
                     /gene="ltrA"
                     /locus_tag="PXB44_001302"
                     /pseudo
     CDS             complement(5478..7198)
                     /gene="ltrA"
                     /locus_tag="PXB44_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(8880..9275)
                     /gene="rpsI"
                     /locus_tag="PXB44_001303"
     CDS             complement(8880..9275)
                     /gene="rpsI"
                     /locus_tag="PXB44_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL5281780.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(9292..9720)
                     /gene="rplM"
                     /locus_tag="PXB44_001304"
     CDS             complement(9292..9720)
                     /gene="rplM"
                     /locus_tag="PXB44_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL5281781.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            10019..10414
                     /locus_tag="PXB44_001305"
     CDS             10019..10414
                     /locus_tag="PXB44_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL5281782.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            complement(10575..12146)
                     /gene="guaA"
                     /locus_tag="PXB44_001306"
     CDS             complement(10575..12146)
                     /gene="guaA"
                     /locus_tag="PXB44_001306"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL5281783.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(12163..>12395)
                     /locus_tag="PXB44_001307"
     CDS             complement(12163..>12395)
                     /locus_tag="PXB44_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281784.1"
                     /translation="ASPLLKLDTERNINLYFQAIHKCTPDKIHTKIINIPSEGNAKEL
                     WTATGCNKTQSFIITYRNDGVGGTFINMTTTP"
BASE COUNT         3529 a         2777 c         2346 g         3743 t
ORIGIN      
        1 tgatttttaa ggtaaagcaa ttttaaacaa gcggtcattt tttcctaatt ttttgcaaat
       61 taacaggcgt gaaattcgca ttctgctcta aatcaagtta tcattaatct acttatttca
      121 cttataaaag gaaaaacgat gtttaatatt agcaaacagc aagttttaga cgcttttcac
      181 ttccgttcgg cctgtcgcag ttacgatcca aacaaaaaaa tcaacaaaga agatatggat
      241 tatattttgg aactcgggcg tctctcacca agctctgtcg gttctgaacc gtggaaattt
      301 ttagtgctac aaaacccgga aacccgtcag aaaatcgccc cggtttcgtg gggaattaaa
      361 cacccaatgg aagaaatgag ccatttagtg gtgattttaa ccaaaaagaa tgtccgttat
      421 gattctgtct atatgcgtga tatgttagcc aaacgaggct taacctatga gcagattgaa
      481 gcctcgctgg tacgctataa gtcgttccaa gaagaggata tgaaattgct tagtgaacgt
      541 gaattattcg attggagtac caagcaaacc tatattgcat taggcaatat gatgaccggt
      601 gcggctctga ttggtattga ctcttgcccg attgaaggtt ttcattacga taccgtaaac
      661 caaattttat cggacgaagg cttatttgac ctaaacgaat atggtgtgtc ttgtatgata
      721 acttttggct atcgcaacaa agaaattaag aaaaaatccc gcaaacctac cgaagaagtg
      781 attgcgtgga ttgaataatc accttaactt ataaaaacgg gcgaattaat cgcccatttt
      841 tatatcttta gttttgacta ctcggctttc acccatttat tggttaatgt gcccgccacc
      901 attgcaccat tcacgtttaa ggcggtgcga gccatatcaa taatcggctc aactgaaatg
      961 agcaagccta ctaactcaat cggcagattc aaggttgata gcaccacaat cgccgcaaaa
     1021 gttgcaccac cacctacacc ggcaatgcca aatgaggaaa ttgccaccac caaaatcaag
     1081 gttaaaatat agtttaagct aaacggatcc attcccactg ttggagcgac catcaccgcc
     1141 aacattgccg gataaattcc cgcacagcca ttttgcccga tggttgcccc aaaggttgca
     1201 gaaaagttgg caatcacact gttattacct aatttattgg tttgcgtctc aatattcaac
     1261 ggaatggttg ccgcactgga acgagacgta aaagcaaaac ttaatgtcgg caacactttt
     1321 ttgtagtaat ccaacgggtt gattttagcc acaaagagta aaataccgtg cacaataaac
     1381 atcaaaccaa tcgcaatgta tgatgccaca ataaagctac ctaaatttac aatatcgctc
     1441 cattgagaag ttgccgccat tttgatcatc aacgcaaata cgccataagg ggttaggcga
     1501 atcacaaaac gtactaaacg cataaccagt ttatttaagg tctctacacc ttgtgcgata
     1561 cgttcgccca acgctttatc ttctttgcct aagctcagtg cagcaatgcc tagcagagcc
     1621 gagaaaataa ccgtgttgat gatcgacgtt gggtttgcac cggttaattc taaaaacgga
     1681 tttttcggaa tgaaagaaag caacattgaa ggcacagata agttgctcac ctgctccaca
     1741 cgccctacca ctttctcttg agcggcaagc tcacgggagc cggcgactaa accctcggca
     1801 gataaatcaa acagatacac taaagcaata ccaatcgctg cggcaatcgc ggtggtcact
     1861 aataacactg atagcacact aaaactaatt ttgcctaaag atcctgcctc ttttaagcgg
     1921 gtaattgccg ataaaataga gacaaacacc aatggcatta cgatcatttg tagcaagcga
     1981 acataaccgt taccaattaa gttaatccaa tcaagagttg catcaacagt agctttttcg
     2041 taaaaagttt gcaaaaaagc accgcttgca agccctaaaa ataacccaat aaacactgtt
     2101 tgaccgagtt tttgcgtaga tttataaatt ttagccaata aaaataatat tgccgcaaag
     2161 acaattaagt tgaaaataag cacagaacct ccaaagtaaa accgcttatt ataaaaatgc
     2221 cgcaagggta ttacgcttgc ggcattcatt caagatgatt tattactttg gtataactaa
     2281 aagttataat aattcccagc ttagattaga gatcaaacgg caaggctcac atctaaaatg
     2341 gaaaatatcg tgcaacggct cagttaattt ccattctccg ccacaacaag ggcatttgcg
     2401 ttgatattca ctttcatcac gccctaaacg gtagaggtaa taaaaggtgg gaatgtgagt
     2461 tttctgctca atttgctgac atagatctcg accgatcatt gcaagatcgc ttgtttcatc
     2521 actaatttga acaagtgcgt ggcactccag cagattgccg ttcatttgta gcagatcgca
     2581 cgcttgccaa ttttgctgcc attccaatat cgcttcgcta agagactgat cctgtaaaga
     2641 ataaagcgga attggggcaa actgctctcc atcataaagt ggcgagcagg tatctaaatg
     2701 ggtggtatat aatatttgaa acgctggcga gcggttatga ctgctttctt cgccctgata
     2761 atctcgcccg acaagctcaa aacttgcaaa aattacgccg atttcgaccg cttgttgcaa
     2821 cgcttcattt acttcatcac tattatattg tggcaaaagg ctttcttgtg taggcacaca
     2881 aacccgagct tgaaactcgt tttgatgaaa agtaatcggc atttctctgc caatgacttg
     2941 cccgttatat aaccattgat ttaagatttg gctaattgcc gtcaccggat cttgctcaaa
     3001 attatgatag ctgaaatgca ctatcgcctg atacattaaa tattctccgc catatcttga
     3061 aattcttgtt tgaccaccgt agtcggctct tgatgaataa tcacgtgaga catcggaaat
     3121 gccgccagca atttctgctc aaggctatca gttatttcgt gagctactag cagcgttaag
     3181 tgatcgtcta attccaaatg taattggata aaacgcactg cacctgcacg gcgggttaga
     3241 atatcgtgaa tccctaaaat gttcggatgt tgcgaagcaa tctctttaat ccgctcaatt
     3301 tcttcatcgg gcaaagcttg gtctagcaag gtttgcaccg cacaccaaaa catttttact
     3361 gcattgaaag caatgtagag ggcaatacct attgcaaaaa ctgcatcggc gtacacaaaa
     3421 ccggcactat taagcaacat tgccactaaa attgccgcat tcattaataa atcagtttgg
     3481 taatgtaacg aatccgcttc aatcgccgga cttttcgtaa gcgtgacaac ataacgctga
     3541 taaaagacta aaagtgcagt aatcacaatt gatacaatac tgacaattac cccaatactc
     3601 gattgagcaa ttaattccgg attggtgagt ttatggaaac cttgtagcag taggaaaacc
     3661 gccgagccgg aaataaacgc actttgggca agtgccgcca aagactccgc tttgccgtgc
     3721 ccaaacgcgt gattctcatc tgccggttgt agtgcaaaac gcagcactaa aatattggtt
     3781 agcgaagcaa acagatccac taccgaatcc gtcattgctg ccaagattga aatcgaaccg
     3841 gttttccacc atgcaaaggc tttaatcaca atcagtaaga tcgcaacaat aatcgctaaa
     3901 ttcgctgcat ttttgactaa tttgctgtat ttttcgttca tttactacat tatttactgc
     3961 attggacttg ccaacccagt tggtttaatt caagagaaag tctagcaatt tcttggctgt
     4021 aagcggtcaa attcggcgat ttttttgcaa ctgtcttgcc aacttcccgc ttatctgcaa
     4081 attgaatctg cccgtaacca caagctaaat tccgcccacg ggttacttct aacatcacat
     4141 tgttaaaacg ttctataggt ggataaatca cactgctgtg taattcattt tgctgaattt
     4201 ccactttcgc ctcaagctcc ggctttttcg caaaacttgc ttggcggaat gcagctcgtt
     4261 gctctaaggt ttgattttta ctgtttttat ctaagaaaaa taaaatgcct ttttgccaat
     4321 tttccggatt tttctcactc ttttctggca aataaagtaa atgttgcatt tcatcaattt
     4381 ggctgtgggt gacttttaca aaagtatcct gcccaaagtg aatagcatca ggcactgcaa
     4441 attgtgggct ttggctgaca caacctgcca atgataacgc aaccaaagtt attccctttt
     4501 tgatagccga cattatgcct gctcctcacg tttaaagact agctcgcttt cactagaact
     4561 gtctgcatca aaccaatagc ccccgtagtt aaactgtttg agctgttcga tgctagtcgg
     4621 ttgggtgtct aatataaagc gaaccatcat tccacgggct tttttggcat agaagctgat
     4681 cactttatat ttaccgtttt tctcatctaa aaacaccggc ttgataatag tcgcatttag
     4741 attttgtggc ttaaccgcac cataatattc atcagaggca agattaacta aaatatcatc
     4801 gccttgttca tcaatcgctt gctgcaaatt ttcagtaata atattgcccc agaaagcata
     4861 aagatctttg cctttcggat tggctaactt agtgcccatt tctaaacgat aaggctgcat
     4921 taaatctaac ggctttaata aaccatacaa gcctgaaagc atacgcaaat gttgttgagc
     4981 atattcaact tgtgctttgc ttaacgtttc tgcctctagt ccggtataaa catcaccttt
     5041 aaaggcaaag agggcggctt tggcattttg ttcgttatgc tcaagctgcc actcggcaaa
     5101 gcgagccaca tttaaagcgg ctaatttatc gctaattgac atcaaactac ctaattcagc
     5161 aggtgaaagc tgtttacaaa tatcaattaa ttcttggcta caagcggtca aatttggttg
     5221 agaaaatgca aaaccgtcaa tattggtttt aaaatcaagg gttttggcag gggaaataat
     5281 ggctaacata aatgcctcta tcaaatagtt aatcagggcg gttattctac cacagtcata
     5341 aagacaaaaa aggcaggaaa ccctgccttc ttgaaaacga aaagaaaagt cgggtagcga
     5401 aagtacatta ctgcacctcc gccccctaag aactgtacaa gcaaatttct ctgcatacag
     5461 ctcaagcctt tcatcagcta tatagccggc ttcacgacct ccaagttcaa agtatgaatc
     5521 tttatgtgac aagtcggatg taccaaacaa cgattgctta atgcatcgct tcctcctaac
     5581 attttgtaca cgatatgatg atcgtgccag cctgtttctt tggttattgg caacccacat
     5641 aatgcacatt ttccacgctg gtctttatac agtgcctgcc attgtcgtct gtgtgattgt
     5701 tcttcataca gacgttcttg gctcagtttt tcgccgtaca actcccattc aggcaaaaac
     5761 ggattatatt cagacttaat tttaacgtgt cgtttgattt taacatagcc acaatagaat
     5821 aagtctatcg gcttagtttc tcctttttca ttggtataaa ctgtgccaaa tacccaattt
     5881 cgagtcgctg tggtacaaaa gtatttctct tttatccaca taataccttt attgtgatga
     5941 cggcgttcac accatttcca aagtactttc caaactaatg cgtccattcg tccgaatgcc
     6001 ttacttgcaa cttggcttcg atgataattt gcccaacctc gtaacattgg gtttagcacc
     6061 attataagat cttcctgttt agccattttc atttagaaat tacctctttt accttgttgt
     6121 agaacgcttt cacattcttt tgacttggtc tgttcagtat ttttcctttg aaacgtctca
     6181 cattccaacc tagaaaatca aaaccttctt caatatgtac cactttcgtc ttttctgttg
     6241 ataaggataa acctcgttca ttcagaaagt tctggataag tgggataact tgattttcaa
     6301 gtaactcttt cgttttacct gatatgataa agtcatctgc ataacgtact aagtaagttt
     6361 tattttctcg aattgtccga ctgccttttc ctccaaaatg agtttctaac agtttttcca
     6421 atccatctaa tgccatattt gcaagcgttg gcgagataat cccaccctgt ggtgtacctt
     6481 cctctgttcg tttcaaccgt ccaaattcca ctacacctga ttttagccat ttccgcaata
     6541 tccgtttatt cattggaata tgctttaata gccagtcgtg gcttatgtgg tcaaaacaac
     6601 ctgcaatatc cgcatcaagt acccactcaa caggttgatt agttttcttg cctttatgcg
     6661 aataaattta gtgtatatga gtaatagcat ctaccgttga acggtttaat ctaaatccat
     6721 aagaaccttt atccgcagtt gtttctgcca caggttgtat tgccaataaa taaagggctt
     6781 gcattgctct atctcgcatt gttggaatac ccaacggacg tttctttcca ttcggtttgg
     6841 ggatatacac tcgtcttaat ggactaggtt tatacccttt ttccgtcaat gatagtgcgg
     6901 cttgccattt tgtctctggg gtctcccaaa gctctttatc gacaccactt gttcgcttac
     6961 cttgattttc agtcactctt cttactgcaa ctaatttagc ataatgtgag tgggttagca
     7021 ttcgttgtaa gtttttaacc ttacgccaat tgccttccag tgttgccttc gcaatccgaa
     7081 tttgcattcc tttcacatac gtttcagctt tatgccaatc gacataatgc catttggaga
     7141 tgtgcgaggt cgctggattg caagctaact tttcagtata atgcttacgc tctttcatac
     7201 ctacctctga tattacaaga taagtcgaaa gtctcagcaa gtgggtagat ccttcaagta
     7261 aacaaatccc tattgagata aactatcctt agtttattcc ctttggttac aagaggatct
     7321 taccgtttta ccgataattt caaccgctct tgtaaccgat tgggttaaag agtcgagcgc
     7381 taggttatga tgagcctaac gctcatttct ttaatttgtt tttgtaccta ttcaacattg
     7441 tattccttga taacattcaa tttctcttct gttatctaca atctctttcg gtgttaccct
     7501 taaaggaaac gctattcatc ttacgatgaa agaccagtta gaagtctgca ccctttcggg
     7561 ttaggctatg tttcacaccc tatctatgcc attacagcat agcattcgct ttttctaacc
     7621 tcccttacct gcattcccaa cagtatcgct tacgctttac ttgccttttc ctcttttacc
     7681 ttcgccatct atttcaaacg gtttgagtat tgtgggcaag gcagaaatac aggcttacca
     7741 agttccatta ctatcacacg agtagtgaag atacagtctc tcccccgaca gtgctttgat
     7801 ggcgtatcct cacgttaaag aagaataacc gactgcctac cttttggtca gagcctatca
     7861 atagctttgg ctctctctcg ataacgaggt ttatcaactg ttcacttacg tttatctttc
     7921 tacccagtct agccccttga aggtgtgcta ttcactttct cccaatgtcc tctcacgatt
     7981 tcattgactg attttcatca ggggttcatt gtcaggaagc tccgcaccac accgttaccg
     8041 gtgtcgcact atccctagac tactgttagc catataacag gttaccttat cattccctcc
     8101 gtactactca tatcttcgga ttgtttgacc gtaacaatga cagtaatagc ttacgcatac
     8161 ttactaagtc agtatcctat actgcataaa taagtccgaa cctttacaaa ataaattcac
     8221 ttatgcgact aaagtcgctt tgtctatatc caaattttcc cgcaaatagc ctataacctg
     8281 tccaataggc gaaggtagct aaccctagct aatgtgagta ttagaggtga ttttaacgag
     8341 aatttaaggt ataattatcc ctagctcata aaaacgaata aacaagagtt tatccttagt
     8401 gtttttattc actattgagg agcattctac gattaccgta gattgcgagt gtgaaaagta
     8461 taagtaaaga tggcactcct tccctaagat ttaccaagtc ttagttctga tgtcttttct
     8521 gagtgctgag gcgatctatt ggactttatt tatagtaggt gttgtaaatt ttctctgctc
     8581 tggggtatgg gtaactccgg tctgcggatc ttgctttctt actctatctt ggtattaaga
     8641 aaaaaaccaa gtacatcaaa gtgaagatca taccttacag cctttcgagt tggttcattt
     8701 actttcccaa cttagaacaa gagtgcttgg gcaaagcccc ttttctcttg cctgtctttc
     8761 caagcactcc gaagagtgct tggaaagtcg gaagcaggtg ataagaatac cacccacttc
     8821 cagcaataac tccttgatat ataaggagtt attgccataa gtacgacaac ttgtcgcact
     8881 taacgttttg agtattgtgg acgacgacgt gctttgtgta aacccacttt tttacgttca
     8941 acgcgacgtg cgtcacgagt aacgaagcct gctgcacgaa gtgctgggcg tagcgtttca
     9001 tcgtattcca ttaatgcacg agtgataccg tgacggattg cacccgcttg accagaaata
     9061 ccaccacctt taacagtgat gtaaaggtct aatttatcag ttaattcaac caattctaac
     9121 ggctgacgta cgatcatacg agctgtttca cgaccgaagt aaacttcaag tgaacgttgg
     9181 ttgatagtaa tgttacctga acccggtttg ataaatacac gggctgaaga gcttttgcgg
     9241 cgacctgtgc cgtagttttg atttgctgct gtcattttta tgctccgtga attagatatc
     9301 taaaacttgt ggttgttgtg cttggtgatt gtgttctgaa cctgcgtaaa cttttaattt
     9361 acggaacatt tcacgaccaa gtggaccttt tggtaacata cctttcactg cgatttcaat
     9421 cacagcttca ggacgacgag caatcatttc tttgaaggtt gcatctttga tgccacctac
     9481 atagccagtg tgccagtagt agattttgtc agtttgtttt ttacctgtta ctgctacttt
     9541 gtctgcattg atcacgatga tgtaatcacc ggtatcaacg tgtggagtgt actcaggttt
     9601 atgtttacca cgaagacggc gtgccaattc agtcgctaaa cgacctaatg ttttacccgt
     9661 cgcatctact acataccagt cacgttgtac tgtttctggt tttgctacaa aagttttcat
     9721 taattaaata ccaaatttaa agtttaaaac ccaatacctc attgaggtat ttcctattat
     9781 tttacggtga acccgacccc ttcgagtctg attcagacca aacttgtgtg gcggggaaaa
     9841 ccacacaaaa acagggtggc gaaattatac agagatttca ctcaaaaatc taacattttt
     9901 tgctgataat ctttcaaaat tgaacctata tcacatttta atcatttgga gcttttcaaa
     9961 taaaaactga aatgttaaaa atagcagtaa tcaatcacta aaacataagg agtttatgat
    10021 ggaacaatgg acaacagatg tttgggcagc cattatggca gcatttattg taggtgctgt
    10081 aataggttgc actctagtta gagtatttaa cagtagtgtc agaaaacagc atgaactgga
    10141 aaaagagttg aagcaaacca aaactcaagt ggatgagcaa aaacaaaaac ttgaacaaca
    10201 ctttgagcaa tcagcccaat tactttcaag tctagcttct gattataaaa agctttatac
    10261 ccatttagca caaagctctg aaaagctatt accagaagca tcaaaaaata ttaccttttt
    10321 ccaacaaacc aaattaccta ttccggcaga aaaaacggaa gaagatcaac ctagagatta
    10381 ttccgaaggt tcatctggct tactgaaatc ataaaactaa aaattgaata tttagtttta
    10441 ctaaaaggca cttagtttaa ggattgattc cgttggaatc aatcctttat tctaaaccgc
    10501 tacattaagc agttaacaac atttttcagc tttagatact gaacttaggt tataagaagc
    10561 ctattttgta agagttattc ccactcaatg gttgccggtg gttttccgct cacgtcatac
    10621 accacacggg aaataccgtt cacttcgtta ataatgcggt tggagatttt gcctaataga
    10681 tcatacggca agtgcgccca gtgagcggtc ataaagtcga tggtttccac cgcacgcagg
    10741 ctgacgaccc agtcatattt gcgtccatcg cccattacgc ccaccgattt caccggcaag
    10801 aataccgtga aagcttggct gactttgtag taccagccgg ctttgtgcag ttcttcgatg
    10861 aagatcgcat cggctttacg cagtaaatcg cagtactctt ttttgatttc gcccagcaca
    10921 cgcacgccta agcccgggcc ggggaacggg tggcggttga gcatttcagc cggtaagccg
    10981 agtgccaagc cgattttgcg cacttcgtct ttaaacaatt cacgcaacgg ttcgactaag
    11041 ccgagcttca tataatccgg caagccgcca acgttatggt gtgatttaat cacgtgggct
    11101 ttgccggttt tgctggcggc ggattcaatc acatcagggt agatcgtgcc ttgtgcaagc
    11161 catttgaccg agctaagttt tttgctttcg tcatcaaaca cgtccacaaa cactttacca
    11221 atggttttgc gtttagcttc cggttcgtct aagcctttta acgcttctaa gaaacggtct
    11281 tcggcattca ctcgcacaat gttcaaaccg aatttgttgc cgaacatttc catgacttga
    11341 tcgccttcgt tcaagcggag caagccgtta tccacaaata cgcagtgcag gtttttaccg
    11401 atagcacgat gtaatagcaa ggcggtgacg gaggaatcca ccccgcccga caaacctaaa
    11461 atcacctcgt catcgcccac ttgctcacga atgcgtttca ccgcatcttc aatgatgttt
    11521 tctgccgtcc agttcgtttc acagccgcaa atgccgacca cgaagttttt cagcaacgcc
    11581 aagccttgtt tggtgtgggt cacttcgggg tggaactgca cgccatagaa acggcggctt
    11641 tcgtccgaca tcgcggcaat cgggcaggtt ggggtaacgc ctgtgatggt aaagtgcggt
    11701 ggtaatttgg tgactttgtc gccgtgactc atccacacgt ccaattttgg ctcgcaagcg
    11761 gtcaaatcat cgtttaaatt tgcaaaaagt tgatcggcag atttgagagc caccgaggca
    11821 tagccaaatt cacggtggtc ggacgtttcg gttaaaccgc ccaactgcat cgccattgtc
    11881 tgcattccgt agcaaatgcc cagcactggc agaccagcgt taaacacata ctccggcgct
    11941 cgtgggctgt tttcctcagt ggtgctttca ggacctcctg aaagaataat ccccgttggg
    12001 ttaaattcac ggatctgctc ttccgttacg tcccaagccc aaagctcgca gtaaacccca
    12061 atctcccgca cacggcgtgc gataagctga gtatattgtg aaccgaagtc taaaattaaa
    12121 attttgtggt ggtggatgtt tgtcattgta atttccttta aattatggcg ttgtggtcat
    12181 gttaataaaa gtgccgccta ccccatcgtt tctatatgta ataataaaag attgcgtttt
    12241 attacaacct gttgcagtcc aaagttcttt tgcattacct tcacttggaa tattgatgat
    12301 ttttgtatga attttatcag gagtacattt atgtattgct tgaaaataca gatttatatt
    12361 tctttctgtg tccaacttta ataaaggact ggccg
//
LOCUS       DANLUA010000068        12322 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLUA010000068 DANLUA010000000
VERSION     DANLUA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            107..1297
                     /locus_tag="PXB44_001308"
     CDS             107..1297
                     /locus_tag="PXB44_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL5281785.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1372..2883
                     /gene="nhaC"
                     /locus_tag="PXB44_001309"
     CDS             1372..2883
                     /gene="nhaC"
                     /locus_tag="PXB44_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5281786.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            3000..6599
                     /gene="putA"
                     /locus_tag="PXB44_001310"
     CDS             3000..6599
                     /gene="putA"
                     /locus_tag="PXB44_001310"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL5281787.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            6708..7358
                     /locus_tag="PXB44_001311"
     CDS             6708..7358
                     /locus_tag="PXB44_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL5281788.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(7359..8246)
                     /locus_tag="PXB44_001312"
     CDS             complement(7359..8246)
                     /locus_tag="PXB44_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL5281789.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(8272..8417)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(8594..9604)
                     /gene="mglC"
                     /locus_tag="PXB44_001313"
     CDS             complement(8594..9604)
                     /gene="mglC"
                     /locus_tag="PXB44_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL5281790.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(9618..11117)
                     /gene="mglA"
                     /locus_tag="PXB44_001314"
     CDS             complement(9618..11117)
                     /gene="mglA"
                     /locus_tag="PXB44_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL5281791.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(11189..12178)
                     /gene="mglB"
                     /locus_tag="PXB44_001315"
     CDS             complement(11189..12178)
                     /gene="mglB"
                     /locus_tag="PXB44_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5281792.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         3545 a         2595 c         2638 g         3544 t
ORIGIN      
        1 agaaaaatta gaagtagatc ataaaatcaa caaaaagtcc gtgcttttta gctaaagatt
       61 gttaaactct tttgtgttac aagtttttaa cataaaggag ttttttatgt ctacttctca
      121 acaactcatc caacaaaccg aacatttcgg tgccaataac tacctacctc tacctattgt
      181 tattagtaaa gctaaaggca tttgggttga agatcctgag ggtaatcgct atttggatat
      241 gcttagtgcc tactcagcgg ttaatcaagg gcattgtcat ccaaaaattg tacaggcact
      301 aaaagatcaa gcagacaaag taacgctgac ctctcgagca ttttataacg atcaacttgg
      361 tatttggtat gaaaaaatct gcaaactcgc taataaagag atggcattac cgatgaatac
      421 gggtgcagaa gcggtagaaa cagcgattaa aactgctcgc cgttggggtt atgatgtgaa
      481 aggaattgca gaaaatcaag cagaaatcat cgcttgtgtt ggcaattttc acggacgaac
      541 aatggctgca gtatctctct cgtctgatcc ggattataaa cgtggtttcg ggccgatgtt
      601 aggtggcatc aaacttgttc catacggtga tttagaagca ttaaaagctg cgattacacc
      661 aaacacagca gcgtttttgg ttgagccaat ccaaggtgaa tctggcattc ttattccacc
      721 tgaaggtttc ttggaacagg cttatgaact atgcaaagag aataatgtgc tgtttattgc
      781 tgatgaaatt caggcaggtc ttgggcgtac tggcaagtta tttgcaacag aatgggaagg
      841 ctttgagcca gatatttata ttctaggtaa agcacttggt ggcggggtgt tcccaatctc
      901 ttgtgtgtta gcaaatcgtg atattttagg cgtattcaat cctggctcgc acggttcgac
      961 atttggtggt aatccattag cctgtgcggt atctattgcc tcaattgatg tactgttgga
     1021 agaaaaactt gcagagcgtt cccgtgagtt aggtgaatac ttcttggcag agctgaaaaa
     1081 aatccaaaat ccaattatta aagaagttcg tggacgtgga ctgtttattg gcttggagtt
     1141 acatcaacca gcacgcccat tctgcgaaaa actcaaagaa aaaggcttac tctgcaaaga
     1201 gacccacgat acagttatcc gctttgctcc ccctttggtg attgaaaaat tcaatttgga
     1261 ttgggcattg gagaaaattc gggaggtgtt ggcttaattg tcattttctc tatattttat
     1321 cctgccaaat cgcctttaac cctttttggc aggaagcaaa acagttgtaa aatgctgatt
     1381 ttgcaaactt tttccaaaaa tcaaccgctt gtaaaaggtg gctttatgtc aaacagaaca
     1441 gaaataacag accaaaatat ccgaatcccg tttcgctatg ctatattgcc tgtgattatt
     1501 atggtatcga ttatggcggt ggctatcatc aaatttgaag cctcacctca cgttccgttg
     1561 cttattgggg caattagtgc cgctgtgatt gcaatggcac atggctaccg ttgggagaca
     1621 attgaaaaag gaatttatag aggcattcgt atggctttac cagcgattgt tatcattatt
     1681 atgattgggc taaccatcgg agcttggcta ggtggtggaa ttgtagctac gatgatttac
     1741 tacggcttga aattgatctc tccgtcgctg tttttagtaa ctatctcaat aatttgtgct
     1801 attgtgaccc ttgcgattgg tagctcgtgg tctacaatgg gaacaatcgg tgtagcggcg
     1861 atggggatcg ggtttagtct cggcattccg gcaccgatgg ttgccggtgc ggtgatctca
     1921 ggagcatatt tcggcgataa aatgtcgcca ctgtcagaca ctaccaatct ggcagcaggg
     1981 attaccgaaa ccaatctgtt tgaacatatc cgccatatgt ttatcactac cgtgccggcg
     2041 ttcctgattg cgttatctgt ctatttctat tttggtatgg aatttagagc cgaaaatctc
     2101 gatcttgcta aaattgaaga cattatgcag gcaatgcagg aacatttctt gatttcgcct
     2161 tggttgctat tagtgccttt attagtcgtg gtgttggtgg caaaaaaagt gcctgcctta
     2221 ccggcattag ctgcagggat aattcttggt tggctctgcc acgtttttgt gcaaggcggt
     2281 gatgtcagcg tagcagtgat tactctgcac gacggctata aaattgagag cggccacgag
     2341 atgattgata agctgttcaa ccgtggtggg atcgagtcga tgatgtacac catttccctt
     2401 acgattgttg cgatgacttt tggtggcatt gcagagcaag ctggaatgtt acattcagta
     2461 gtggaaaaaa tccttaccct tgcaaaatca gcaggtagct taattagtgc tacgattgta
     2521 tcggcattta ttaccaacgt aacgacatct gagcaatata tttctatttt attaccaggc
     2581 agaatgtata taaaagccta tagggataag ggactaagtt cgagaaacct ttctcgtgca
     2641 ttggaagacg gaggaacagt tacttcggtt cttgtacctt ggaatacctg tggtgtttat
     2701 gcgttctcaa tgttgcaagt gagtgctttt gaatatgcac cgtatgcaat aatgaattgg
     2761 ctcattccac tgattgcgat tggcttggca accttgaata tcaaaattga gcgattgcca
     2821 ccggaagaat tagccaaaga acctgaggaa attactctgc caaaacgtgg ttgttgtcca
     2881 taatttatct aaatagtatt taataacaat aggttaagtt aaataacaag cggtcggttt
     2941 tatcttaaaa tttgcaagtt tatacataaa tttgatcgct tgctgtatag aaaggaaaaa
     3001 tgatgtcata ccaactttta cctaacacca ctatccgtcc tgaattgagc cgtcattacc
     3061 gctttgatga gcaaaccctg gtgcaaaatc tgatagaaca agcacattgt gatgaacagg
     3121 ctgataacat taaaaagctc actacccaat tagtcggtaa agtccgaact gcacgccaga
     3181 aagcaagcgg ggttgatgcg ttaatgcacg aattttcact ctccagccaa gagggggtgg
     3241 cgttgatgtg ccttgccgaa gcattactac gcattcctga caccgccact gcggataaat
     3301 taattcgaga caaaattgcc aaaggggatt ggcgttcaca caccggcaac agcccctctc
     3361 tgtttgtgaa tgcagcggcg tggggcttgg tgattaccgg caaattggtt tcaacccaca
     3421 gcgaaagctc actctcatca gcactttcac gcctgatcgc caaaggcgga gaaccgctga
     3481 tccgcaaagg tgtggacgta gcaatgcgta tgctcggcaa gaaatttgta acgggcgaaa
     3541 ccattcaaca ggcgattaaa aacggcgaaa aacgcttcgc aatgggttat cgttacagct
     3601 acgatatgct gggcgaagcg gcactcaccg ccgaagatgc caaacgctat tacaacgact
     3661 atgttgcctc tattcacgct gtgggtgcca cctcgaaagg cttgggcgtg tataaatcct
     3721 cgggtgtgtc ggtcaaactc tccgccattc accctcgtta ttccctttcc caacaccaac
     3781 gtgtgatgga cgaactctat ccacgtctga aagaactgtt cttgctggcg aaaaagtatg
     3841 acatcggctt gaacattgat gccgaagaag ccgaccgcct cgaattgtcg cttgatttga
     3901 tggacagact gcttgccgac cctgatttgg cagggtttaa cggcattggt tttgtggtgc
     3961 aggcgtacca aaaacgttgc ccgtatgtga tcgagcattt aattgagacc gcccgtaaac
     4021 ataatcgcca gctaatgatt cgcttggtaa aaggcgcata ttgggacact gaaatcaagc
     4081 gggcgcaaat ggacggtgca gagggctatc cggtcttctc ccgtaaggtg cataccgatt
     4141 tgaactacat cgtttgtgcc aaaaaattgc tggcggcgca agatgtgatc tatccgcaat
     4201 ttgccaccca caacgctcaa actctcgcca ccatttacca cttggcacag ggcaaacgtt
     4261 ttgagtttca atgcttgcac ggtatgggag agagcttgta cgaccaagtg gttggcagcg
     4321 ataatcttaa cgtgcaatgt cgcatttatg cccctgtcgg tacttatcaa accctgcttg
     4381 cctatttagt acgtcgcttg ttggaaaacg gagcgaacag ctcatttgtt agccaaattg
     4441 tggatgaaaa tctcagtgta gatgatctgg ctcaagcccc tgttcaaaaa gcagaactga
     4501 ccaatggcac tatgcacccg aaaattccat tgccagtcga tttatttgac gacaatcgtg
     4561 ttaattccaa aggctatgat ttaactgatg cagtaagcct acaaaattta gaaaatgccc
     4621 tcaatgaact taccaaacag cattaccgtg ctgaaccaca gctggcaaat gataatacaa
     4681 gcggtcaaga aagccgaaaa atttacaatc cagcgaacca agctgaatta gtcggcgaag
     4741 tgattgatgc caccgaaaac gatatggaac aggcgtttga aagtgcggcg gcgttccaaa
     4801 gtgaatgggc gaatacttcc cctgcggtgc gtgccgagtg cttagaaaaa atggcggact
     4861 tgatggaagc caatatgcca caactgttcg atttggcggt gcgtgaggca ggcaaaacgc
     4921 tcaacaatgc aattgctgaa gtgcgtgaag ccgtcgattt ctgtcgttat tatgccaaag
     4981 aggtacgcaa caacattgaa gcatacaaaa atccgcgtgg cattgtagta gcaattagcc
     5041 cgtggaactt tccgttagcg atttttgtgg gtgaagtttc atctgcgttg gtggcaggca
     5101 atgtggtact agcaaaacca gctgaacaaa cttcgttaat ggcacattat gctgtcagcc
     5161 ttttctacca agcaggtgtg ccgaaagcgg cgttgcaatg cttaccgggt agaggaaaaa
     5221 cggttggggt ggcgttggtg gcagatcatc gtgtgaacgg cgtgattttt actggttcaa
     5281 cagagacagc taaaaacatt aaccgcaatt tgcacaaaaa cgagaaaatt ataccgctta
     5341 ttgccgaaac tggcggacaa aatgcaatga ttgttgatag ctcggcactg ggcgagcagg
     5401 ttgtgataga tgttttaaac tccgcatttg actcagcagg gcaacgttgt tcggcattgc
     5461 gtgtgctgta tctgcaacgt gatgttgccg atcacattct cacaatgctc aaaggagcaa
     5521 tggcggagtt gaaagttggt cttccacaac acttaactac cgatgtcgga ccagtaatcg
     5581 ataaagtcgc acaacaacgc ttgctcgatc atattgaaaa tctcaaaaaa tcggcgaaaa
     5641 gttactatca ggtggctatc tcctctgaaa ttgccgaaaa tggtattttc gtaccgccaa
     5701 cgctgttaga aatagatcat atcagccagc ttaaacacga agtattcgga ccggtgctac
     5761 acgttatccg ttttgatgcg gataaactcg accaagtgat tgccgacata aactccacag
     5821 gtttcggtct aaccagcggt ttgcatagcc gaattgatga aacggtggat ttatggttga
     5881 aatctgtcca cgctggaaat ctgtatgtaa accgtaacac ggtaggggct gtggtcggcg
     5941 tacagccatt cggtggaatg gggttatcag gaactgggcc gaaagctggc gggccgctct
     6001 atctacaacg tttagtaaac cgccaagaat ggacattgaa cggtttacag ggcaatgcac
     6061 aagcggtcga ttttgactta aattttgcaa tccgcaaggt tctaagtgga gctgagcagc
     6121 aaacggcgtt agatctggcc aaaagccttg cacgccgctc gccactcgga aaaacctacc
     6181 caatgcaggg tattaccggc gagaaaaacg agatgatttt cgtggctcgc ccacagattg
     6241 ggattagcaa aggtaatttg gcttcacagg tgcaagcctt gattgccatt gtaacggtcg
     6301 gcagccaagc ggtggcagaa aaatcaagcg agctggcaaa acacgcctct gcctttggta
     6361 agaaattaca ggtggtggat gatttcaaca gtctcgatca cctcagcaca ctcattgtgc
     6421 ttgacctgct ttctagtgag gaaaaattgc atcatgccga acgcaaaggg gcgattttga
     6481 cctacatcga agggctggat ttagcgctct cactgctgcc gttggtacac gagaaagcaa
     6541 tcagcatcaa caccgctgcc gcagggggaa atgccagctt aatgagtgaa atggattaag
     6601 caacaaacaa aagagtataa gagggcagat cacaatgatc tgccttttta ttttaagggg
     6661 ctgtcctaga taacgactaa aacttccatt taggttaaga taaccctatg agaaaaagtc
     6721 gtctaagtca gtacaaacaa cataaactca tagagctgtt tgtagcaggc gtaatcgctc
     6781 gaacagccgc agagttagtg aatgtaaata aaaccaccgc agcctattat tttcaccgtt
     6841 tgcgattgct catctatcaa catagccctc atttggaaat gtttgaggga gaaattgaga
     6901 ttgatgagag ctatttcggc ggtcatcgca agggtaaacg aggtcgtggt gcagcaggca
     6961 aaaccgccgt attcgggctt ttaaagcgtg acggcaaggt ttataccgta gtagtgccga
     7021 acacacaatc tgctacactt ttgccgatta ttcgggaaaa ggtgaagccc gatagccttg
     7081 tttacaccga tttttatcga agctatgatg tgcttgatgt gagtgaattt aaccattttc
     7141 gtatcaatca cagcacacac ttcgcagaaa atcaaaatca cattaacgga attgagaatt
     7201 tttggagtca agcaaaacgt catttacgca agtttaatgg tattccgaaa gcccattttg
     7261 agctatattt gaaagaatgc gaatggcgtt ttaatcacag tgacttaaaa tctcaaatat
     7321 tccttttaaa acaattggtt aaaggggggt taggttagtt atctaggaca gcccctaaaa
     7381 gtaatataac cgatatttag gcctgacttt tcaaataatt gaatatcttt attcgctttt
     7441 aaagcagaca aatctgacgg gttcggataa ggcatcgctt ggcattcgtt cttctccaat
     7501 ttagctaaac gtactgaggc atcgggcgta atactaaata caaggcgatc tatcttcgct
     7561 tttccttccc aatagtgttc aaatgctgta aatcttaccg ctgcgtcctt ttggtaacca
     7621 tagaactgga atgcacctgt gcctataggt tctaaatcta atttttccgg cgtacccgct
     7681 atttgcattt tatttgcata ttccgcagaa aagatcgcca taaaatccat agctaaatta
     7741 gcaagaaagg gggcattcgg ttcagataat tcgattttta ccgtgtaatc atctactttt
     7801 gttacatttt tgatgatgtt ttgcatattc attccgtaga aaaactcata gttaccgccg
     7861 gacaatttgt gatatggatt cgtcttatca aactgacggt taatcgaaaa aataacatca
     7921 tcagcattaa aataacgact aggggtaaaa cttttattac tctggaactg cacattctga
     7981 cgcaagaaaa aagtataaat ttttccgcct tcactgattt cccatcgctc tgccagagaa
     8041 ggagtgaggt tagtggtacc tttttcaaaa tcaatcaggc gattatagat tgcttgcccg
     8101 acatcaagag tagctccatc tgttgcaatc tgaggattca atgctgccgg agatgattcc
     8161 aaacagtaag taaaagattt aggtgctgcg aaagcagaac ctgtaaatgc taaagaaata
     8221 agtgcaagtt gtgtcagctt gttcattgtc atatccttaa atgttaagca ataaaaaacc
     8281 ctttcaaaag ttgccttccg aaagggttag aattgggttt gcgattttgg attgcgtttg
     8341 ccatttcagg ttttcggaag atatcttccg aacacgccaa atgcccgaaa gattaatcgg
     8401 tcaaatggta gtgatgatgt ttaacgcaat agctcataat attctccaaa attgaatgtt
     8461 taggaattta cgctttttaa aaatcaattg caaacgtttt ctttaaaatt ttgtaaaaaa
     8521 ttcaacattt ttcaccgctt gtatcgtacc taggagcaaa tggcgatttg ctcctaggtt
     8581 tccaagcaat aggctatttt ttcttcgcat atttaagtga gtcaatcgct accgccaaga
     8641 taatgatgcc acctttaata atatattgcc agtaagggtt tactccaata taggttaagc
     8701 cgtagttgat aacggtgaag attaacaccc ctgttactac accgatgata gtacccacac
     8761 caccggcaaa ggatacccca cctaccacac aagcggcaat ggcatccatt tcatacataa
     8821 agcctaagtt gttggttgca ctaccgatac gacctgcttc taacataccg ccgaaggcat
     8881 agaagatgcc tgaaagcata taaataccca tcaggttaag cggtacgttt acaccggaaa
     8941 ctcgtgctgc ttcagggttg ccgccaatag caaatacgtt tttaccgaag cgggttttat
     9001 tccataacgt ccacataata atggtagcaa tcacggcata aatagtgatg taggatagcc
     9061 taaagtcgcc aaagcggaag aagccttgtg caaaggttga gaaatcatcg ctaaagcctg
     9121 caattggcga tgagcctact gcatcgtagt aaagcgagtt gataccatac acaataatca
     9181 ttgttcccat cgtggcaata aacggtgtta cgttgaggta agccaccaca aagccgttaa
     9241 ataaaccaat gacagcaccg atggcacaca cggttaatac aaccacagga atcgggattt
     9301 ccgagaggtt tgggaatacc ttgttcatat tttccattga ttggagcaag gtggcggaaa
     9361 ttaccgctgc caagcccact tggcgaccgg cagataagtc tgtcccttgt gttacaatca
     9421 agcctgcaac ccctaatgca ataatcaaac gcacagaaga ttgcgataaa atgttactga
     9481 agttacgcaa gctcaagaaa cttgggtctt gcacaataat cacaataagt aagattaata
     9541 atacgaagta aatcgcattt tgtttaaaaa aatcgagtgt tctattttgt tgtaaagcag
     9601 acatacttca ctccttgtta taaatatttt gctgcgagtt gcaagatttc ttcttgcgag
     9661 gtgtgagcgg tttcgacaat acctgcaact ttcccgttac tcatcactaa aattcggtct
     9721 gtgaccccaa gcaattccgg catttccgat gaaatcataa tgatgccttt atctttattc
     9781 gccagctcta aaatcaactg gtaaatttca aattttgccc cgatatcgat accacgggtc
     9841 ggctcgtcaa gcatcaagat ttcaggctgg gttaacaacc aacgcccgat aatcactttt
     9901 tgttggttac cgccggaaag tgagccaata tgcgtttgat gcgatggggt ttttacattc
     9961 atcgaatcaa ttacccattg ggtatcgctt ttcatttttt tattgctcag taagccgaac
    10021 ttgcctagat aagacttcat atttgaaatc agagagttaa actcaatgct taagttggca
    10081 taaatccctg ttgaacggcg ttcttctgta accaatgcaa agccgttatt aatcgcatca
    10141 aaagcgttgc ggttattgat ttctttatcg tttagtcgga tagtgccgcc tgctttctcc
    10201 cgtacaccga aaatggtttc aacaatatca gtccgttttg ctccaactaa gccggcgatg
    10261 cctaaaattt cccctctgcg taagctaaag ctgacatctt gaatagaggg ttgatttttt
    10321 gccgttaaat gctcaacttc aagaatcact tctttcggca gattggtttt ttcagggaaa
    10381 cgctgggtca gctcacgccc taccatcatt gaaaccactt gatccattga ggtatcttta
    10441 attgcaacgg tgtttaccca tttaccgtca cgcaaaatgg taatttcatc acaaatttta
    10501 aaaatttcgt ccattttgtg tgaaatatag ataatgccac agcctctgtc tttcagtttt
    10561 tggataattt taaataaatg ctcgacttct ttttctgaaa gtgacgaagt tggttcgtcc
    10621 ataatcacaa ttttggcatt gtaagagaaa gcttttgcaa tctcgatcat ctgcatttgt
    10681 gaaaccgata atttggctac tttatcacgt ggatcaacat caatatctaa ttcatcaaaa
    10741 atcgccttag tatcacgata cattttatcg tgatctacaa aaggcccttt tagtgggtaa
    10801 cgacctagcc ataagttatc cattaccgtc acttgtttta ctaggttcaa ctcttggtga
    10861 accattgaga tcccattttc taacgcctct tttgaggtct taaaattcac cggcttgcca
    10921 agaaagagaa tatctccctc atctttagca taaatcccaa ataagcattt cagtaacgtt
    10981 gatttacctg ctccattctc tcccattaag gcgtgaacac tatgggattt caccgttaag
    11041 ttagcattat ctaaggcttt taccccggga aatgacttgc taacatttgt cattgtgagt
    11101 aaaacttcac ctgtcatatc tcacccctga ttacatcgaa aaatcgaaaa aaagcacggg
    11161 tatccatcac ccgtgctaaa agcgtgaatt attttaaaaa ttcagataag ttgtctttat
    11221 cgacaccaac gtaaggaata cgcaccacac ggttttccaa tttccattgt gtaccagcaa
    11281 ctgcatcttt accgtctgct aagttagcag aaagctgaac aaccgcttta ccttggttta
    11341 caccatcatt taataccgta ccggctaatt cacctttttt gattaattga agtgcttctg
    11401 gcaatgcatc tacaccgaag attggtaatt ttctaccgtg agctttggtt gcttctaatg
    11461 cacctaacgc cataccatca ttattagaaa taattacttc gatgtctttc gctttaccac
    11521 ttgataacca agcatccact ttatctttag cttgggctgc atcccataat gctgcgtcca
    11581 taaagatttg ctcactgtcg ataccttgtg cttttaagct ttctaccaca tatttagtac
    11641 gtgcttccgc atcagggtga cccggctcac cttttaataa ggcaaattgg attttaccgt
    11701 ctttattcaa gtctaattca ggatgggctt tccactgttt tgcaattaac tcaccttgaa
    11761 taatgcctga ctctttcggc tcagtaccta cataataagc tttcggatag ctatcaatcg
    11821 cagctgcacc cgggtcttta ttgaagaaca ccacaggcac atcttcactt tttgcttttt
    11881 gaataattgt tttataagct gatggatcca ctaagttaat tgctaatgct ttaacccctt
    11941 tagaaagtaa tacatcaact tggtcgtttt gaatagattg cgtattttgc gagtcattca
    12001 ttaataactc gactttttgt gttttaccct ctttttcgat ttctttacgc attaatgcca
    12061 taaagttatc gtcatattta taaattgtta caccaatacg gttttcagct tgagctgtac
    12121 tgatcgttgc acctaatgcg actgccattg ccactgcact taatactgct ttcttcatag
    12181 tagatcacct cttttatgtg tttataggga aatgaattgc ttaccataag caacttggtg
    12241 caaagtataa ctaattttaa aaaaacaatc cgtgatcatt ctcacagaat taagacaaag
    12301 tggagaaatt tttaatttta ag
//
LOCUS       DANLUA010000069        11980 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLUA010000069 DANLUA010000000
VERSION     DANLUA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(95..1228)
                     /locus_tag="PXB44_001316"
     CDS             complement(95..1228)
                     /locus_tag="PXB44_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5281793.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1359..2387)
                     /locus_tag="PXB44_001317"
     CDS             complement(1359..2387)
                     /locus_tag="PXB44_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281794.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2399..3943)
                     /locus_tag="PXB44_001318"
     CDS             complement(2399..3943)
                     /locus_tag="PXB44_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5281795.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(3994..4932)
                     /locus_tag="PXB44_001319"
     CDS             complement(3994..4932)
                     /locus_tag="PXB44_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL5281796.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5264..6775)
                     /locus_tag="PXB44_001320"
     CDS             complement(5264..6775)
                     /locus_tag="PXB44_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5281797.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            6994..7818
                     /gene="iolB"
                     /locus_tag="PXB44_001321"
     CDS             6994..7818
                     /gene="iolB"
                     /locus_tag="PXB44_001321"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL5281798.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(7894..9345)
                     /locus_tag="PXB44_001322"
     CDS             complement(7894..9345)
                     /locus_tag="PXB44_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5281799.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(9523..10833)
                     /locus_tag="PXB44_001323"
     CDS             complement(9523..10833)
                     /locus_tag="PXB44_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5281800.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            complement(10849..11544)
                     /locus_tag="PXB44_001324"
     CDS             complement(10849..11544)
                     /locus_tag="PXB44_001324"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL5281801.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            11760..>11980
                     /locus_tag="PXB44_001325"
     CDS             11760..>11980
                     /locus_tag="PXB44_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5281802.1"
                     /translation="MKNIETKWLEDFLTLEECRHFSQAAEKRNLSQSAFSRRILALEE
                     VVGVKLFDRTSIPLQLTEQGKLFHSQTRN"
BASE COUNT         3449 a         2783 c         2355 g         3393 t
ORIGIN      
        1 taaaaatttc tttaattgaa ctcataattt ttccttaaat gtaaaaaaac gtacctcaac
       61 caatttgaga tacgtttcta ttttaatgta attattaaat ctcactcacc tccacccacc
      121 ttttctcctc cgccgatttt gctatcgctt ccaatactct ggagatttta tagccttctt
      181 caaaatcggg atacatcggg tcatcagcgg cgatgccgtt gatgaggtca cggatttcca
      241 ccgctttttg gtcgttaaaa ccgatgccgt gaccggcact cacacaaaaa gcagcgtagt
      301 cgggatgttc cgggccgact aaaatggttt tgaatccttg gcgggattta tcgtcatcgt
      361 gcaaatagag tttgagttct gccattcgtt cttgggtata gctgagcgtg cctttagtac
      421 ctgtaacgac ataggtcagc cccattttac gcccgcagga aatgcgggag ctttcgagcg
      481 tgcccattac cccattagca aatcgtatga gactacttgc ctgatcttcg ttttctactt
      541 tgattttttc tgctggattg tgcggactag ggcgttcttc aatcactgtt tgcatatcgc
      601 cgataacacg ctcaatgtct gaaccaacca aatagtgtgc gatactgaca atatgtgcgg
      661 ctaaatctcc caacgtacca agcccggctt tggctttgta gcagtgccaa tcaatcgggg
      721 tattagggtt ggcgagataa tcttcgttat gcgtgccgta gaaatgcacc acctcaccaa
      781 tttcgccgtt ttgaataatt tggcgagcca gttgggtggt tggatttttg atatagttga
      841 aaccgaccaa ggttttaact cccgcttgtt gagcggcaat caccatttct ttggcatctt
      901 gggcggtgag tgcaagcggt ttttctgaat agacgtgttt gccgtgttta atcgcttcta
      961 aggcaatttc tttgtgcaga aaatttggtg tacaaatgtc caccacatcg acattcggat
     1021 cggtaaccaa ttttcgccaa tcgtcagtag cacgctgaaa gccaaattct cgggcttttt
     1081 gatcggctaa tgcttggttg atttcggcaa ggtattccag ttggattttg cctttaagtg
     1141 gaaacagggt tggggcttga gcgtaggcaa tggcgtggca gcgaccgata tagccggtgc
     1201 caactaagcc gattttgatg gttttcataa tactatccca gtagggtgcg tttgctaacg
     1261 caccattaga attttcagtg cattggcaaa cgcaccctat aagaaagcgg tctggttttg
     1321 caaaattttt acaaaatcag accgcttgtg ttagctatct ataatgctcc tcgtctgctc
     1381 ttcgcatagg tgtcgaaggc aacggcaagg atgatgatta agcccataat gatttgttgg
     1441 tagtaagcgg aaacgtccat tagtactaaa ccgttaatca atacacccat aataatggag
     1501 ccgatgatgg tgcctgtgac tcgtccgtat ccccccatta gggaagttcc gccgataacc
     1561 actgaggcaa ttacacgtag ctcaaaagaa acgccggcaa cggattcggc actgcctaaa
     1621 cgggcggaga gaatgaaacc ggcaagtcct gccaatgctc cgatcaccac ataaacactg
     1681 actaaggtac ggttaatatt cacaccggct aaacgggctg cttccggatt gccacctacc
     1741 gagtacacat agcgtcccca gcgggtttta tgcaatgcga agtaaccgat aatggcgact
     1801 acggcaaaaa tcactaccgg caccggtacg cctaaaatat cgccacgtcc ccaccagcgg
     1861 taaccttcgt caaatccggc aattggcgag ccgttattta acacaagtgt taaaccacgc
     1921 caaattgtca taccgccaag cgtgataata aacggtggca gtttgagttt agtgacgcct
     1981 aagccgtgta accaaccaat gaaagtgcct aaacctaaac agatactcaa acctatcagc
     2041 cagctcattc cgccccaagc accggcttct agcgttaccg tattatcgcc tttgataata
     2101 taggccgcga acattgcggt aacagcaagg attgagccga cagagagatc gatccctgca
     2161 gttaagatga cgaaagtcat tcctaccgcc ataatgccga aaatcgacac ttcggttaaa
     2221 acgttataga tgttgcgttg cgataaaaat gccccgtttt gtgaagtgaa gaaaataatc
     2281 agtaagatca agaaaatcag tacgccgaaa cgttcaaaga aggcgataaa atcgattttt
     2341 cctttgtcat cgaccatacc catatttttt agaaattgcg ttgtcataca aacctctctt
     2401 atgctgccgc tttacgctga acgccaacag ccatcattga cattaatttt tcttcggtgg
     2461 catcatcgcc gtgaatttcg ccagttaccc gaccttcgga aagagtgata atgcggtcgg
     2521 aaatcgccat aatttccggt aaatcggagg aaatcactaa taccgcaaca ccttgttttg
     2581 ccatatcaaa cagcacttgg tgaacctcag attttgcccc aacatcaata cctcgtgtcg
     2641 gctcatcaac aattagtact ttcgggttga gtgccataca acgggctaaa atcacttttt
     2701 gctgattacc acccgataat ttgcgggctt caagttcgct acttgccatt ttgatgcgta
     2761 acgcttggcg gtaggtttcg attaaatcat cttctttttg attatcgaca aacaagttac
     2821 ccttcaataa tttgcttaaa atcggcaggg tcatattggt tttaatcggt aaaccgagaa
     2881 ctaagccctc ttttttgcga tcttccggca caagggcaac ccctttttcc aaagcatcta
     2941 acggcgaggt ggcagcataa ggcttaccgt ctaggctgat ttcatcagcc gtataatttt
     3001 ctaccccaaa caagcaacgg gcaatttcag ttcgccctgc gccaactaag ccggcgatcc
     3061 ctaatacttc accctcgtga atgttaaaag agacaccgtg gagagcaatg ccgtgtggat
     3121 cgaatgccgg tttttcccga tgcagatttt tcacagctaa acgtattggt cggtgggtat
     3181 ggtgagtctc ttctggtgga cggcgagtaa aggcaacttc acgtcctacc atcattttaa
     3241 taatttgatc gacattcaca tttttgactt caccctcacc agaatagcgc ccgtcttgga
     3301 aaacggtgaa acggtcgcat agggcaaaca cttcgtttaa gcggtgggtc acataaatca
     3361 cgctaatgcc acggcttttt aaatcacgca cgatttggtg tagtgtttcc acttctttat
     3421 cacttaaggc agcggacggt tcgtccataa taatcaactt agcgtttaag gtcattgcac
     3481 gggcaatctc gaccatctgc tgttgtgcca cacttaaacg ggaaacttgg gtgtcaggtt
     3541 tgatatttaa gccgagatta tccagcacta attgtgcttc ctcattcacc gctttttcat
     3601 tgacgaataa acctttcacc ggctctctgc ccaaaaagaa attttcagca accgtcatat
     3661 tcggtaacaa gttaaattct tgataaatcg ttacgatgcc tttgagttgg cgatcaaacg
     3721 gggaatcgct caaacttaag gtttcaccgt taaagataat ttcgccactt gtttgtggct
     3781 gagcgcctga aagtgctttt aataaggtcg attttcctgc accgttttca cccaacaatg
     3841 cgtgaacttc gccttttccc acagtcagtt tcacgccatt gagtgccata acaccagaga
     3901 aactttttgt cagatctttc acttcaagta agggagtaga catacaacct ccgtaaaaaa
     3961 acgccctccg gcctcagccg gaaggcagat aaattattcg ctgatttctg caaaacgttc
     4021 tgcttcttgc agattatctt tcaggataat ttttggcgga tagtccgcac cgctcacttc
     4081 tttgctctca cggatattgc caaccacttg ctctaatgcg gtgcttaccg caaaacttgg
     4141 gcgttggtca gcggtgagat acaaccagcc atctttcaca cgtgcaagag cctcaggggt
     4201 tgagtcaaag ccggttacta aaatatcgcc ggctttcaaa ccttggctac gcaatgcttc
     4261 aatcgcacca agtgccatat cgtcattcgc tgaaataatc acttctggtt tctcttttaa
     4321 ggttggtaac acactttcaa taatgcgtaa tccctcagaa cggagccagt taccggtttg
     4381 atcaaccacg attttatatt tgtcaccacc ggcagctaat tcatcacgaa tacctttggt
     4441 acgttcaatg ttagaggttg aacccggctg gccggttaaa agaataattt ttgccccatt
     4501 cgggtatttg gctttcactg cttttgcgac ttcttgaccg cctgtgtagt tatttgcccc
     4561 aaagtgtgga accggcttac tgctttcaac ttttcggtct aatgtcgcag caggaatttt
     4621 ttctttgata atttcttcca ctgcacctga aatcgcattc acatcattcg gggaaatgat
     4681 aatgccttta gcaccacggg taattgcatt ttctaagtca gaagcctgct tagtagaaga
     4741 actttgcccg tctaatactt gcaatttgat ctccaacttt ttactggttt ctaccgctac
     4801 tttttgtagt tgaacttcaa acgggctgga taggttcggt aagctaaata ccacaagctc
     4861 atctttcgca aagctgttca ttgctaagcc taacgttaaa gcactcaata caaatttaat
     4921 acgctttttc attacaaaat cctcttttta agatgggaat tgacggcgaa tattgccaat
     4981 gttacttcaa actgcgaagg aaacttaagt cttatttcaa taataaacct caatgaaata
     5041 aatttttcaa tttagaatat atcattcaaa attttccaaa ccaaatgctt tgtttcaaat
     5101 ttgttacgta gatcacattt ttaggaagag ataaatgcaa gcggtcaaaa caggtcgctt
     5161 ttttgcaaaa atttgaataa aaatgaccgc ttgtgagatg aaaaccaata aaaaagcggt
     5221 gatttctcac cgctaaacac tcaaaaaaga aatgtactcg ttattataag gttgggaaat
     5281 taaatgccgc ttggctacgc accgagctgt ccggccaacg ggttgtgatg gttttcatac
     5341 gggtgtagaa acgcacgccg tctgtgccgt acacattgag aggcccgaaa atagaggctt
     5401 tccaaccgcc aaagcagtgg aacgccattg gcacaggaat tggaatattc acgcccacca
     5461 taccggcttg tacatcttgg gtgaattgac gtgctgaatc gccatctgcg gtgaaaattg
     5521 cactaccatt gccgtattgg tgactgttaa tgagtgcgat cccttcggca taatctttca
     5581 cacgcacaac agagagaacc ggtccgaaga tttcatcttg ccagatggtc atttcaggtg
     5641 tcacgttatc aaacagcgta cctccgacaa agtaaccgtt ttcaaaccct tcgactttat
     5701 agcctcgacc atcgacacag agtttcgccc cctgtgccac cccttgatcg atatagctct
     5761 caattttggc tttatgctct ttggaaatca ccggccccat atcattttca agcacgcctt
     5821 gtggtagtaa gcctgaaccg attttcaatg ctttgacctt aggaattagc tcttcaatga
     5881 ttttatcggc taattcatca ccaacaacta ccgctaccga tagtgccata caacgttcac
     5941 cggctgcacc aaatgctgcg cccagtaagg cattaattgt gccttgtaca tcagcatccg
     6001 gcataatcaa agcgtggttt ttcgcaccgc ccaaggcttg aacccgtttg ccgtttgctg
     6061 agcctttctc ataaatatat tgtgcaattg cagttgagcc gacaaaactc accgcctgaa
     6121 tgcgagagtc ggttaaaaga atatctaccg cttctttatc accttgcacc acattgaata
     6181 caccgtccgg caagccggct tcttttaata attctgccaa gcgaatagac acacttgggt
     6241 ctttttcaga cggttttaaa acgaaagtgt taccgcaagc aagtgccact gggaacatcc
     6301 acatcggtac cattgccggg aaattgaacg gggtaatgcc tgccactacg cccaacggtt
     6361 gctggattga gtgaatatca atgccacgcc ctgcattggc ggagaactca cctttttgta
     6421 agtgtggaat accagtggca aattccacca cttctaaacc acgggttaat tcgccaatcg
     6481 catcggaata gattttgccg tgttctgagc tgattaaacg agccaactca tcaaagtttg
     6541 cttccaataa ctctttgaat ttaaataaaa ttctggcacg gcgtagtggt gattgttgtg
     6601 accagctttt gaaagcctga tcggccgctg caattgccgc atcgacttcg tttggcaggc
     6661 ttaatttgac ttttttggtc tcttcaccgg ttgcgggatt aaataccgag gtaacacgct
     6721 ggctttggct ttcaacgact gcaccgttaa taaaattttg gacaaattgg ctcattggag
     6781 gctccttatc aactgattga gaggaaaaac ggataaaata cagccgataa actaccgcta
     6841 aaaatggaaa atatcaatac ataatactaa aaaatgaaaa ttttatttca tttttgtgat
     6901 ctaagtagca agtttacaga ttgccggtat taaaatgaga caaaatttcg tatagtttga
     6961 aagcgtattc catttcgtta agcgaggttt cctatgtcta gtccattatt gtctaaaccc
     7021 aatttcaacc aacatgaagg gttaattcag cacattaccc ctgaaaatgc acagtgggaa
     7081 tatgttggat ttgatgttta tcagctcagt aagcaacaaa ttacgctgga aagtggtgaa
     7141 aatgaacttt gtctagtctt tgtgaccggt tttggctctg tctatgttga tgattatgct
     7201 tttgaaaata tcggcaaccg taccacgcca tttgaaaaaa ttccgcctta tgcggtctat
     7261 atcccgccac acaaaagcgt gttggttaaa accgaactca atttagaatt ggcagtgtgc
     7321 aaagcaccaa gtcagggtaa ttatccggtg cgtttgatta gcccgaacca tatcggtgtg
     7381 gaaaaacggg gctttggcaa caatcaacgc ttggtgcata atattctgcc ggaaaccgaa
     7441 gcagccgaca gtttgctggt ggtggaagtc tttaccgatg aaggcaatac cagttcctac
     7501 ccaagccaca agcacgatga caaacactct accgaagaaa cctatttgga agagacctac
     7561 taccaccgct tcgatccacc acaaggtttt gcaatgcaac gggtttatac cgatgatcgc
     7621 acacttgatg aatgtatggc ggtgtatgat aaaaatgtgg ttaaagtgcc tcgtggctac
     7681 caccctgttg ctaccattgc cggctataac aactactacc taaatgttat ggcagggccg
     7741 gtacgcaaat ggaaatttac ttgggaacaa gatcatcttt gggtgaacag tgaaacctat
     7801 aaagccaaat ttgagtaatg caagcggtct ttttccacca attttttaca aaataaggga
     7861 ttaaaaatgg gacgcttttg gcgtcccatt cggttatact ttcatacaaa gatattttat
     7921 ttctaaaaac tcttcaatac cgtacttcga accttcccgc ccaagtccag actctttcca
     7981 gccgccgaac ggagcgattt cgttactgat gatcccctca ttaatcccga caatgccata
     8041 ttccaactgc tccgatactc gccaaattcg cccgatattt tggctgaata agtaagccgc
     8101 caaaccaaac tcagtatcat tcgcccactg gataacctct tcttcggttt caaatttgaa
     8161 aatcggggca aggggagcaa aggtttcctc acgagccact ttcatcgctt gagtgacatt
     8221 tttcagaatg gttggctgga agaatgtgcc acccagtgcg tggcgttcac cgcctaacac
     8281 cagttctgcc ccaagtgaaa gggcatcgtt aatgtggctt tcgacttttt ccaccgctcg
     8341 ttcactaatt agtgcaccta tcgtgacctc ttcctcaaat ggcgagccaa gcttgatttg
     8401 agccactttc tcactcaatt ttgcaagaaa ttgctcataa atgcccgctt gcacataaat
     8461 tcggttagca cagacgcagg tttgcccacc gttacggaat ttagaggcaa acacgccctc
     8521 aaccgctgta tctagatccg catcatcaaa cacaatcgcc ggtgcattac cgcctagctc
     8581 caatgccaat ttttttaccg tccctgccga ttgttgcatt aaaattttgc cgacacgggt
     8641 ggagccggta aaggtgagtt tgcgtacggt cggattagtg ctcaactccc gccctacacc
     8701 ttcgacatcg ctactggtaa taatgttaaa tacccctttg ggaatacctg cttgatccgc
     8761 taattgggct aatgctaagg cagaaagcgg ggtttctgcc gccggtttaa tcacaatcgc
     8821 acaacctgcc gctaacgccg gtgccgcttt acgggtaatc attgcatttg ggaaattcca
     8881 cggtgtaatt gctgccacca cgccaatggg ttgtttagtc acaaacaagc ggcgaccggc
     8941 tttatcttgt ggcaagacat cgccataaat ccgcttgctt tcttcggcaa accactcgat
     9001 aaaactggct ccataggcaa tctcgcctcg actttctgcc aatggtttac cctgttctag
     9061 acttaaaatc tgggcgagtt cttcttggtt ttgcataatc agctcaaacc atttacgcaa
     9121 aatcacggaa cgctctttgg gcaataatgc ctgccatttt ttctgggcaa ctgcggctgc
     9181 ttcaatcgct tgagcggttt cttccgtgcc taaatcagcc acctcaccga ttttctcacc
     9241 attagtcgga ttctgtaccg taaaggtttt cccattttgg cttgaaacaa atttaccgtt
     9301 aatataggct tggaaacgga gtagcaaaaa tttgtgattt ggcataatca ctccttgaat
     9361 ggttaaaaag agaacaatat tgactataat ccaatttcag taaagagaaa agagctgaaa
     9421 aataactttt tactcaataa taaagcacct tagacaagcg ttcttctaag gtgcagccat
     9481 ttattattga gaattaactt aaattctcta catatttaat aattaagata caatcattgg
     9541 agcccatacg aatccaaaag caagcccaac agcaacccca actaaagctg gaagcataaa
     9601 cggatgatct ataatatatt tgccagcacg agttgaaccc gtagtatcta attccatcgc
     9661 tgctaatgaa gttgggtagg ttggcaggat aaaaatgcct gtaattgcaa tgaaagaagc
     9721 caaaattgcc cagttaggga tccccaagct tactgctaat ggaataataa gagcagaagt
     9781 tgacccttgt gaataaagta atgcacaagt aaaaaagagt actacagcta acaacattgg
     9841 atattcagtt aaaatatttc caatagaggc tttaatttct attaaatgtg catcgacaaa
     9901 agtcatacct aatgtaacaa tcccgataat taccgcaata gaagtcattc ctgagcggaa
     9961 aattggacta gccacaatcg tatcaggttt aatttttcct accaacatca ttaagcacga
    10021 agaggctaac atcatcacaa taattatatc tcgggtactc attgacttcc cattttcata
    10081 tactggcttt aaccctggaa cggctgcaag tagaacaacc aatacagttg ctagtaagaa
    10141 aatgccaatc gatagttttg ctccttttga ataggtagat tcactcgcta acttacgaga
    10201 tgggctgacg attcctgctt ttaaacgttc taaataaaca acatcatttt gcaattcaca
    10261 gccctgctta gaagcaacaa atgcagcaac catagaggca ataaaagaaa caggtaagca
    10321 tactgctatt acttgaaaaa atgtaacact gttgttttcc attactgcta tcatagccgc
    10381 cattgcagca ctcaatggag aacctgcaat agctaattga gaagacacaa ccgagccggc
    10441 taatgctctt gaagggcgaa taccactctc ttttgccact tctgcaatca caggtaaggt
    10501 ggaaaagacg ataaaacctg taccagccat aatcgtcatt aaccaagtaa tcatcggtgc
    10561 aataaagtta atatatttag gatttttacg catcaatact tccgcataac gaacaagtaa
    10621 atccatacct cctactgctt gtaacacagc agcacctatt acaacagtca taataatcag
    10681 cattacatca actggaatag aacccatagg taaaccaaaa cctagagtta gaatcgcaag
    10741 ccctaaacca ccatatagtc ctattcctaa gcccccagaa cgcagtccta aatagattgc
    10801 agcaagtacc acaaagattt ctgcataaat catatccatc tccttaaatt aatgagtttg
    10861 ataccaatca attgcttttt taaccaatac caacgttgaa tctacataac gcattggctc
    10921 acgctctatt tcttgagcta aaataatagg tacttctgta caacctaaaa taattacttc
    10981 tgcacctaaa cgaatgagtt catcacgttg ttgtaacatt agctctgtgg ctttctctaa
    11041 ctcacctgat tttaataaat agatactatc cattactgcc ttttgaccat tttctgtcgg
    11101 gcaaataaaa gtcagtcctt gtgcttcaag ctgttttttg tataggtcta ccgccaaagt
    11161 tgcatcagtt gccaacaatc caacccgcca ttttccacac ttcaaaacct catcaaccac
    11221 acaatcaatc atacttagca tctctacctg acttttggcc ttcaagtgtt caaaccaata
    11281 atgtgcagta ttacatgcaa taatgatgca ggttgcccct gcactctcaa gcccttcaat
    11341 atatttttcc attaccggag ccggattctc tccatttgct aaaatatagc gagttctatc
    11401 tggtatgtcg ggaatagagg aaatcaataa tggaatatga gcttgatcac aatcagctgg
    11461 tgttaagcgg ataaattttt ggaacatatc tgctgttgct gcaggtccca ttccacctaa
    11521 aatgccaata atatttcttg ccatacactt ctccttataa gatttaattt actgcgatga
    11581 cttatacaaa caagttatgc aaaaaagaaa tgcaataaat cgaatcagta tgcaaaaaat
    11641 gcaacaaagc tctttataga tagaaaaaaa atctttaaaa tcaataaaat actaatatat
    11701 caaatataaa tttaatgcat ttaatgaaat cataatgcga aatttgcatt ggttaggata
    11761 tgaaaaacat agaaacaaaa tggttagaag atttcttaac gctagaagaa tgtcgccatt
    11821 tttctcaggc agcagaaaaa agaaatttgt cacaatctgc atttagcaga cgtattttag
    11881 ctttagaaga agttgtggga gtgaagttat ttgatcgtac ctctattcca ttacaactca
    11941 cagaacaagg taagctattt cattcacaaa cgcgaaactt
//
LOCUS       DANLUA010000070        11945 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLUA010000070 DANLUA010000000
VERSION     DANLUA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            50..313
                     /locus_tag="PXB44_001326"
     CDS             50..313
                     /locus_tag="PXB44_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5281803.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            329..859
                     /locus_tag="PXB44_001327"
     CDS             329..859
                     /locus_tag="PXB44_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5281804.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(875..2002)
                     /locus_tag="PXB44_001328"
     CDS             complement(875..2002)
                     /locus_tag="PXB44_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL5281805.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(2006..3520)
                     /gene="xylG"
                     /locus_tag="PXB44_001329"
     CDS             complement(2006..3520)
                     /gene="xylG"
                     /locus_tag="PXB44_001329"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL5281806.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            complement(3587..4588)
                     /gene="xylF"
                     /locus_tag="PXB44_001330"
     CDS             complement(3587..4588)
                     /gene="xylF"
                     /locus_tag="PXB44_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5281807.1"
                     /translation="MKLKSKLLAIATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            4830..6149
                     /gene="xylA"
                     /locus_tag="PXB44_001331"
     CDS             4830..6149
                     /gene="xylA"
                     /locus_tag="PXB44_001331"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL5281808.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            6302..7747
                     /gene="xylB"
                     /locus_tag="PXB44_001332"
     CDS             6302..7747
                     /gene="xylB"
                     /locus_tag="PXB44_001332"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL5281809.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPPPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(7810..8133)
                     /locus_tag="PXB44_001333"
     CDS             complement(7810..8133)
                     /locus_tag="PXB44_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL5281810.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            8257..8799
                     /locus_tag="PXB44_001334"
     CDS             8257..8799
                     /locus_tag="PXB44_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL5281811.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            complement(8970..9794)
                     /gene="dapF"
                     /locus_tag="PXB44_001335"
     CDS             complement(8970..9794)
                     /gene="dapF"
                     /locus_tag="PXB44_001335"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL5281812.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            9943..11265
                     /locus_tag="PXB44_001336"
     CDS             9943..11265
                     /locus_tag="PXB44_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5281813.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            complement(11313..11672)
                     /locus_tag="PXB44_001337"
     CDS             complement(11313..11672)
                     /locus_tag="PXB44_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL5281814.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
BASE COUNT         3486 a         2548 c         2456 g         3455 t
ORIGIN      
        1 agtaaaaata ggtatattat ctaaatcgtt aagcgaaagg agttatctaa tgagtacatt
       61 ggaagatatt aaaaaaacag cgaaagactg gcatagggcc gacatcatcg ctgctttaca
      121 taaagcgggt tggtcgctta gacaactctc attgaagcat ggctacagtc aatccagcac
      181 cttgaaaaac gctgtagatc gtccgtggtt aaagggtgag cgtattattg ccgaagcaat
      241 cggtatatca cccgaagaaa tttggcctga acgttatgct caacgaaacc ataaaaaatt
      301 tgccgacaga tagcttaaag aggtttttat gagtgaaaac agtctaaaaa cacactattc
      361 ggcaaaagaa ttggctgagt taagtttagc ctgtttacct aaaacaaagc aaggagtttt
      421 gtatcaggca aagaaacaag gatggactac tcataaaaag gttggagttg ggggagaaat
      481 tttagaattt gcactctcaa acctccctca agaaatccaa accgaaatcc gctcccgctt
      541 tatgtcggcg gtggtggaag ccaagccgaa aaagttaccg gcagtgcgtg cggaggtgga
      601 tgtgggaaac ctgaccacca aacagcgaga aattgctgat gcacgaatgg cactggtggc
      661 gtatgtgttg gaactggaag gctcaatgag ccggattaaa gcgataacct acctttgcaa
      721 ccttgccaaa caaggcgaaa tgccaccgca cttggtggag ctggttgccg tagcaaatgc
      781 taaaaagacg gcaaaacgca caatagttcc catttttagc ccgattatgc cggatttccc
      841 gttggatagt cgagattgac cgcttgttat ccaattaggc ttttttcttg ctgatggtat
      901 ctacccaaac tgcaagcagt agaattccac ctttaacgat atattgccag aaggttggca
      961 catcaagcat actcataccg ttatccagca aagcaataat aaatgctcca ataactacgc
     1021 cataaatgct accgataccg ccggctaaac tggctccacc aatcacgcag gcggcaatcg
     1081 catctaattc ggcattttgc ccggcggaag gtgcgccggc tccaagacgt gagcttaaaa
     1141 tcaagcctgc aatggcaact aataagccgt ttaaggcaaa aatcgcaagt ttgacttttt
     1201 caactgaaat ccccgagagg cgagcggcat caatgttgcc accaatcgca taaatgtgac
     1261 gcccgaaggc ggtttttctg gccaagaatg tgccggcaat ggctagaatc accaacacca
     1321 ataccgggaa tggcacacca cggtaatcat tgagtaggta gatcgcacct aatacgataa
     1381 ttgcaactag tccgtatttc agcgtgtctt tcgacataga aggcacagta agattgagtt
     1441 tttgtctggc tttgcgttgg tagctacccc aaacaataaa gcaaaatatc ccgatagtac
     1501 ctaaaatcat acccaccata tcaggcaaat agccttgacc aatggttgtc atttcattgc
     1561 taatcggtga aacggttgtc ccgttggtta agccgaccaa tacaccacga aacgccagca
     1621 taccggcaag agttacaata aaggacggca ctttttgata agcaacccac caaccgctcc
     1681 aagcacccag catgacgcct aaaattaaag taactataat agttaacgag agcggccagc
     1741 cccaccatac attactgatg gcggcaaatc caccgagtaa gcccattagc gagccaaccg
     1801 ataaatcaat ttctgcggaa ataatcacaa ataccatacc gatggcaaga ataccggtaa
     1861 tggaggtttg gcgtaacagg ttggagatat ttctggcact taaataagca ccgtcagttg
     1921 caatagagaa aaagctcata atcacaacaa ttgcaatcag cataatataa acttgtaaat
     1981 taatcgattt taacttattc ataggttact ccttgagtgc cgcttccatt actttttctt
     2041 gggtaaggtc ttggttaatc aaatctgctt taattttgcc ttggtgcata accaatacac
     2101 gatcactaat gcctaaaact tcaggtaatt cggaggaaat gacgataatt gccatacctt
     2161 ctttagctaa ttggttgatg agcttataaa tttcatattt tgctcccacg tcgataccac
     2221 gggtcggctc atctaaaatc aggattttag ggttaagcaa taggcatttt gccaaaattg
     2281 ctttttgctg attaccgcca cttaaccgtc caattgctaa ttcaggcgag gaagttttca
     2341 ctttcagttt tgcaatagat tggttgataa tggtttcttc cagcggttca tttattactt
     2401 ttttgccaaa acaaaattgc ggtaatgaag agagagttat gtttttaccc acccccataa
     2461 ttggaatgat gccgtgtttt ttgcgatctt ccggcaccat cacaatatgg ttttcaatcg
     2521 cttgtgcaca atttttgatt ttaatcggtt gcttatgcag aaaaatatca ccttgatatt
     2581 tgccttggta agagccaaaa atacattgtg ccatctcggt tcgccctgaa ccgaccaaac
     2641 cagcaatacc taaaatttca ccttggcgta gtacaaaatt agcattatca acccgtttga
     2701 tatgtgtatt ggttgggtgc caagcggtta aattttcaac tcgaaggact tcttcaccaa
     2761 tattgtgtgg ctcgtgtggg tagagcgagg taatctcacg tccaaccatc atcgtaataa
     2821 tatcgtcttc actcattccc tctgcactgc gagtaccgat atgttcgccg tctcgaatga
     2881 cacagatttt gtcggaaatg gctttcactt cattcaattt gtgtgaaata taaacacaag
     2941 caatattgtg agcttgtaaa tctttaatta aactcagcag aatctcggtt tctttttcgg
     3001 ttaacgaggc ggtaggctcg tctaaaatca acaaccgtac ctgtttattc aatgctttgg
     3061 caatttcaac caactgttgt tgccctaaac ctagctctcc aactttggtg tttgggtcaa
     3121 tatccaactg cacttgttca agtaaggttt tacaacgcag atacatttca ttgtcatctg
     3181 taatccctga tttggtaatt tcattcccta aaaacatatt ctccaaaatc gacatatttt
     3241 ttactaaggt caattcttgg tgaataatcg aaatgccttt ttcttcagta tctttgatat
     3301 ttctggcaat caatttttcg ccggaaaaat agatttcccc ttcataatca ccggcaggat
     3361 aaattccaca taatactttc atcagagttg atttgcctga accattttca ccacataaag
     3421 aaagtatttc gccggtttca agggaaattg aaatattatt taatgccaca acctcaccga
     3481 attttttagt gatgttcttc atttctaata atttcgccat acttagttag ccccttattt
     3541 tcaaaaatgg cgaggaagcc tcgccatcta gaaaaattaa agtgatttat ttatatactg
     3601 catctttggt atggaagccg tctttaatta ctgtgtcatc aatattttct ttagttacaa
     3661 caaccggctc taataaatat gccggtactt ctttcatacc gttatttaat tgagcattca
     3721 tttctacttt ctcatcttta cctaatgcaa cagcaatttc ggcagctttg tcggctaaat
     3781 tggtaattgg tttatacact gtcatcgttt gcgtaccggc aataatacgt ttaattgcgg
     3841 ctaaatctgc atcttgtccg gaaattgcaa ccttgctgga taacccttgg gcacttaacg
     3901 cttgaattgc accgcctgcg gttgcatcgt ttgaagctac aactgcatca atattatttt
     3961 tattagcagt tagtgcattt tccataatct gtaatgcttt ttcagcaagc caagaatcta
     4021 cccattgatc gcctaccact ttaatttttc cactgtcaat taacggttgt agcaccttca
     4081 tttgaccttt acggaacagc ttcgcgttat tatctacagg tgaaccaccc attaggaaat
     4141 aattcccctc cggcttttgc ccaataatgc ttttagcctg caattcacct actttttcat
     4201 tatcaaatga aacataaaaa tccacatcgg cattattgat taagcggtcg taagcaagta
     4261 ccttaacacc ttctttcttc gcctcggcaa tcacattgga taacacttcg ccgttaaacg
     4321 gaataatcac caatacatca atatctttat tcaacatatt ctcaatttgt gagatttgtg
     4381 cggttgcatc gccatttgcc gattgaacga agacttttgc tcctaagctt tccgctttat
     4441 tcacaaaaat atctcggtct ttttgccaac gctctaagcg taaatcatca attgacatac
     4501 cgatttttag atctttagca acagaagcat tgctgagtac cattaaagtc gccgtagcaa
     4561 tggctaataa tttagactta agtttcatag ttcacctcat tggttatgaa aatgtgagca
     4621 tctttttcgc ccgtaagcag tctgggctta tttcaaatga agttcaatta tcatatttat
     4681 taatatcatt atgatttttc acttttgtga tctacgccac attaaccaat aatcgaaatc
     4741 caaaaacgaa atatcgtaat tgaaggtttt gcataatttt ccgatgatgt taccgaaaga
     4801 ttatttttcc aattcatagg agcattatta tgtctaacta tttcgacaaa atctcaaaag
     4861 tacaatatga aggggctaat tccaccaacc cgtttgcatt caaacattac aacccggatg
     4921 aagtggtttt aggcaaaacg atggcagaac atctgcggtt agcggtttgc tattggcaca
     4981 ccttctgctg gacgggtaac gatatgtttg gcgtgggttc gcttgacaga agctggcaaa
     5041 aaacggggga tttactgcaa ggtgcgaagc aaaaagcgga aattgccttt gaatttttcc
     5101 aaaaattagg cattccttac tactgcttcc acgatgtgga tgttgcaccg gaaggcaaca
     5161 atttcaaaga atatcagcac aatttcaaca ctattgtgga tattttagag caaaaacaag
     5221 ctgaaacagg cgtgaaatta ttatggggta cggcgaactg ctttaccaat cctcgctata
     5281 tgtcgggtgc ttcaaccaat ccgaatccgg aagtctttgc ttgggcggca gcacaagtct
     5341 ttaccgcaat gaacgcaacc aaacgcttag gcggcgaaaa ttatgtatta tggggcggac
     5401 gtgaaggtta tgaaacccta ctcaacactg acttaaaacg tgagcgtgaa caaatcggtc
     5461 gctttatgca gttagtggtg gaacataaac acaaaatcgg ttttaacggc actttattaa
     5521 ttgagccaaa accacaagaa ccaaccaaac atcaatatga ctacgatgtt gccaccgttt
     5581 acggtttctt aaaacagttt ggtctagaaa aagagatcaa agtcaacatt gaagcaaacc
     5641 acgccactct tgcagggcat actttccaac acgaaattgc gactgccacc gcattagata
     5701 tttttggctc gattgatgcc aaccgaggtg atccgcaatt aggttgggat accgaccaat
     5761 tcccaaacag cgtggaagaa aacacccttg tgatgtatga aatcttaaaa gcaggtggtt
     5821 tcacaacggg cggtttcaac tttgatgcga aaatccgccg ccaaagtacc gatccgtatg
     5881 atttattcca cgcacatatc ggggcaattg atgtgttagc cctttcgcta aaacgtgcgg
     5941 caaaaatgct ggaagatcaa gcattacaga aagtagtaga taaccgctac gctggctgga
     6001 accaagattt gggcttacaa attctaaacg gtaaagcaag ccttgaagat ttggcaaaaa
     6061 tagtggaaac ccaagggtta gatccaaaac ctgtttccgg tcagcaagaa tatttggaaa
     6121 atttggtaaa tagctatatt taccgttaat ctacaagcgg ttgtttttta tcaatttttt
     6181 gcaaagttaa gctcgatctg atccccctct ttgctaaaga ggggtgatag atctccaaaa
     6241 cttaaaattg caaatgttcc gaataaaatg accgcttgta ttcaataaca aaggaatatt
     6301 tatgtacatt ggaatcgatc tcggcacatc gggtgtgaaa gtcgtgttac tcgatgaaaa
     6361 tcaacacatt attgccatca cacaaaaatc acttcctatt tttcgtccac acccattgtg
     6421 gtctgaacaa aatccgcaag attggtggaa tgccaccaat gaagcgatgt tagaactttc
     6481 cgctactcaa gatttacaag cagtaaaagc gattggttta accgggcaaa tgcacggagc
     6541 gactttacta gataacgcag ataatgtact ttcccctgcg attttatgga atgacggacg
     6601 tagttttatg gaatgcgaag agttagaaca gcttgtgcca aatagccgtg aaattacagg
     6661 caacctgatg atgccgggct ttaccgctcc gaaattgcgt tgggttgata aacaccagcc
     6721 cgatattgcc gaaaaagtca gcaaagtctt gctaccgaaa gattatctcc gcttgttgat
     6781 gagcggcgaa tatgcctctg atatgtctga tgcctccggc acaatgtggc ttgatgtggg
     6841 taaacggaat tggaacaaat cgctactaaa tgcttgcgga ttagacattg aaaatatgcc
     6901 gaaactgttt gaaggcaatc aaatcacagg ttatttacgt ccaacccttg ccgagcaatg
     6961 gaaaatgaaa tctgtgccga ttgtggcagg cggtggcgat aatgctgccg gtgcaatcgg
     7021 cattggctta taccaaaccg ggcaagctat gctctcattg gggacttcag gggtttattt
     7081 tgtagtgagc gataaatttc aggctaatcc gcaaaaagca gtgcatagtt tttgccacgc
     7141 tttacctgat cgttggcatc tgatgtcagt aatattaagt gccgcttctg cggtagattg
     7201 ggcagtaaaa tcattgggac taaaagatgt atcgacttta ttccaacagg ttgaagcaag
     7261 ccaaacggca tctgatgcta ttttcttacc ttatttatcg ggtgaacgta caccgcataa
     7321 tgacccttat gcgaaaggcg tattttgggg attaagccat aatgacaacc aaataacgat
     7381 ggcaaaagcc gtgattgaag gggtaagttt cgctttggct gaagggattg aggtactcca
     7441 cgaaactggt gtagttgctg acaatattgc attaattggc ggtggagcaa aaagtgtgta
     7501 ctggcgacaa ctacttgccg atattagcgg taaaaccttt gaataccgca ccagtggtga
     7561 tgtcggtccg gcattagggg cggcaaaact cgctcaaatt gcgctaaacc caaatcagga
     7621 tattgctacg ttctgtcagc cgttacccct ggaacaggtt taccaaccac cccctgcccg
     7681 ctatgcagaa tatcaacaaa aacgccaaaa atttactgag ctttaccggc ggttaaaggg
     7741 gttataattc ttgccaacta ttacaagcgg ttgattttgc agattttttt gtaaaatcaa
     7801 ccgcttgtat cacactttag aaaacaaatt aatcaccaat acaccggcaa taatgaggct
     7861 gatgccgatt aagcctgcca agtcaatttt ctgtccgaaa gcaaaataag caacaagggc
     7921 ggtaaggaca atccctacgc cggaccaaat agcatacacg attccgaccg gtagggtgcg
     7981 gaaaataata gagagaaagt aaaaggataa accgtataag gcgagcgagc caatagtcgg
     8041 tatagctttg gtgaagccgt tgcttaattt gagtaaattg gtggcagcga tttcgaggca
     8101 gatggaaatc gcaagtaaaa tccaaacgtt cattgatgac ctctttgcgt aaatgcgtgc
     8161 tattttacct gagttgcaaa aaaatttaaa aaaatcaccg cttgtttttt cctgattaag
     8221 ctatggtaca atctttggtg aatcaggagg tgtgaaatgg cacaggttta tatcgtacac
     8281 ggttatacgg cgaatgccga taagcattgg tttccttggc tggagcaaga attagcgaag
     8341 tgtggtgtca gttgtgagcg gttgaatatg ccggattcgg caaagccgtc attagatggt
     8401 tggttaaatc atcttgcaca aaatgtggag ctgaccgatc aaacggtttt tgtcgggcat
     8461 agtttaggtt gcattgccat tttgaatttt ttagccaaaa attacgccaa gattaaaggg
     8521 gcggtgtttg tgtcggggtt ctatcaaccg gtagaaaact tacccgaact gtctgccttt
     8581 accaactatt atgcgatttt gccgagcgta tcgagctttc caagctatgt ggtaagtgcg
     8641 ttagatgata aggtcgtgaa tcaccaatac agtgatgcgt tagcccaaca tttgcacgcg
     8701 gattatattc ggttaaatca aggcgggcat tttttggata gagaaggtgt taccgaatta
     8761 cctgtggtgt tggagttggt gaagaagtta ctcgagtgaa acctgttttg gtacaagcga
     8821 ggacgcttgc accatctata agttgaattt aaacaaaagt tatacatcag tctgatagtg
     8881 ccagcgtcca cgctggtact tttgtataaa ccaaccctat tttgcaaaaa aacggtaaaa
     8941 ataaaccgct tgcctcccta tgggaaaact tacagtttaa taaatccatc ataaatgtgg
     9001 gtggcatcgc cggtcatata cagaggatgc cctactcctt gccattcaat aatcagcgtt
     9061 ccaccgggta aatccacctg cactttgtta tctaacaagc cttgcataat gccgaccgcc
     9121 actgtgccgc acgcaccgct accacaggct tgggtttcgc ctgctccacg ctcatacacc
     9181 cgtaatttaa cgtgattgcg attcactact tgcataaagc tgatattcgc ccgttcagga
     9241 aaacgctcgt ggctttctag taacggcccg agttcattta ccggggcggt tttcacatca
     9301 tcaacttgca gtacgcagtg cggattcccc attgaaacca cgccacataa cacggtttgt
     9361 agctcggtgc gtaaaatata gtttttctca aatttatttg ctgtaaaagg cacttgcgaa
     9421 ggttcccaaa tcggttcgcc catattcaca cgcactttgc cgtcttcttg caaacttagc
     9481 accattttgc cttttgcggt gctaacgtga atgtcttttt tgttggttaa gcctttgagc
     9541 gtaacaaaac gggcaaaaca tctcgcccca ttgccacatt gcgacacttc actgccgtct
     9601 gcattaaaaa tgcgatagtg aaaatctaat tcagggtcgt aaggcggttc aaccaataaa
     9661 agttggtcga aaccgatacc acgatggcga tcagaaagcg tacggatcat ctcttcggta
     9721 aggtaaacgt tttgggttac accatcaacc accataaaat cattgcctaa gccgtgcatt
     9781 tttgaaaatt gcatattatt ccttaattaa ttagaaattt tattgattta gattatagag
     9841 attagtttta tttattgata tagtacgtgc cgattaattt gatgaaaaat gcagattttt
     9901 tcaaacacag agtttcattt acttatttag gagcaaaaaa ttatgtctgc gatgtttatt
     9961 atccaatttg ccatagtgtt actatgcatt ttggttggcg cccgtgtagg cggtatcgga
    10021 ttaggggtgt tcggtggctt gggcttagcc attctttcat ttgggtttgg gctaaaacct
    10081 gccggtttac cgattgatgt aatgtttatg attatggcgg ttgtgtcggc tgcagcagcc
    10141 atgcaagcag ccggtggttt ggactatatg atcaaaattg ccactcgcat tttgcgtaaa
    10201 aacccgaaat acattacctt tatcgcaccg cttgtaactt ggacattcac cgtgcttgcc
    10261 ggtacaggtc acgttgctta ttctgtatta cctgtaattg cagaggtaag ccgccacaac
    10321 ggtatccgcc ctgagcgtcc actttcaatg gcagtaatcg cctcacaatt tgcgattgtc
    10381 gccagcccga ttgctgcggc agtagttgct gtggttgcct tccttgaacc tcaaggtgtt
    10441 acattgggta acgtgttaag tgtgactatt cctgcgacct tattgggctt aggtttagct
    10501 tgtgtgtttg taaataaaat gggcaaagaa ttaaaagacg acccaaatta tcaacgctta
    10561 ttgcaagacc ctgaatatgt gaaagaaaat cacattacta ccaatccaac cgaattaccg
    10621 ttaaaaccga ctgcgaaatt atcagtaggc ttattcctat tcggtgcatt attagtggta
    10681 ttaatgggcg caattccagc tcttcgccct gtgtttgatg gtaagccgat ggggatggct
    10741 cacacgattg aaattgtgat gttaagtatt ggtgcgttga ttatcttctt ctgcaaaccg
    10801 gacggtaatg aaatcaccaa aggttctgtc ttccacgccg gtatgcgtgc agtgattgcg
    10861 attttcggta ttgcttggtt aggcgatacc ttaatgcaag gtcatatgga cgaagtgaaa
    10921 tcaatggttt caggcttagt tgaaaccgca ccttgggcat ttgctttcgc attattcgta
    10981 ctttccgtat tggtaaacag ccaaggggca acggttgcaa ccttattccc tgtagcgatt
    11041 gctatcggca ttcctgcacc ggtgctaatc ggggtatttg tggcggtaaa cggctacttc
    11101 ttcattccga actacggccc gattattgcc tcgattgact tcgacaccac cggcacaacc
    11161 cgtatcggta aatatatttt caaccatagc tttatgttac cgggcttact tagtatgatt
    11221 ttcagtatcg gatttggttt attactttcc aatatcctac tttaatcgct cgcaaaacct
    11281 cccgtttggg aggtttttta tttgcaaaat tttcagcaaa aacgaccgct tgtaacggct
    11341 cttgcaagca ttacacctat caaagtaagc aaaagcccac cgattaaatg taaactcagc
    11401 actgtcacaa actgccccca tttttctgct aatagttttt ccgttagttc taaagagaaa
    11461 ctggaaaagg tggtaaaact gcctaaaaaa ccgctgatga acagcaaccg ctggctctcg
    11521 cctaaattaa accccattgc aataccaatt aacaaacaac caagccaatt tgctgctaaa
    11581 gtaccaaagg cgaattggct gagtatcggg tttagccaaa cggctaattg ccagcggcac
    11641 atagctccaa gtaccgcccc gattgaaatc ataagtattg aagacatcat ttttcctcat
    11701 ctaacgtttt ctcacatcat acctaaaaat gagtgattta gaaaaataat gtgatggatt
    11761 tcacaaattt gaaacttcct atttgcttaa aaaatgccgc tttggcaata tcactccgaa
    11821 atatgttcat attcaaacat tttttcatca ctttccaatc attttcatct taaggagaaa
    11881 ctttatgagc aaatccttac gtaatgcctg tattggcgaa tttatcggta caggttttat
    11941 tcttt
//
LOCUS       DANLUA010000071        11791 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLUA010000071 DANLUA010000000
VERSION     DANLUA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(51..437)
                     /gene="rplQ"
                     /locus_tag="PXB44_001338"
     CDS             complement(51..437)
                     /gene="rplQ"
                     /locus_tag="PXB44_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL5281815.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(473..1462)
                     /gene="rpoA"
                     /locus_tag="PXB44_001339"
     CDS             complement(473..1462)
                     /gene="rpoA"
                     /locus_tag="PXB44_001339"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL5281816.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(1493..2119)
                     /gene="rpsD"
                     /locus_tag="PXB44_001340"
     CDS             complement(1493..2119)
                     /gene="rpsD"
                     /locus_tag="PXB44_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL5281817.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(2148..2537)
                     /gene="rpsK"
                     /locus_tag="PXB44_001341"
     CDS             complement(2148..2537)
                     /gene="rpsK"
                     /locus_tag="PXB44_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL5281818.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2554..2910)
                     /gene="rpsM"
                     /locus_tag="PXB44_001342"
     CDS             complement(2554..2910)
                     /gene="rpsM"
                     /locus_tag="PXB44_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL5281819.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3048..3161)
                     /gene="rpmJ"
                     /locus_tag="PXB44_001343"
     CDS             complement(3048..3161)
                     /gene="rpmJ"
                     /locus_tag="PXB44_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL5281820.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(3186..4505)
                     /gene="secY"
                     /locus_tag="PXB44_001344"
     CDS             complement(3186..4505)
                     /gene="secY"
                     /locus_tag="PXB44_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL5281821.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(4508..4942)
                     /gene="rplO"
                     /locus_tag="PXB44_001345"
     CDS             complement(4508..4942)
                     /gene="rplO"
                     /locus_tag="PXB44_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL5281822.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(4947..5123)
                     /gene="rpmD"
                     /locus_tag="PXB44_001346"
     CDS             complement(4947..5123)
                     /gene="rpmD"
                     /locus_tag="PXB44_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL5281823.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(5130..5630)
                     /gene="rpsE"
                     /locus_tag="PXB44_001347"
     CDS             complement(5130..5630)
                     /gene="rpsE"
                     /locus_tag="PXB44_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL5281824.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(5645..5998)
                     /gene="rplR"
                     /locus_tag="PXB44_001348"
     CDS             complement(5645..5998)
                     /gene="rplR"
                     /locus_tag="PXB44_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL5281825.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(6013..6546)
                     /gene="rplF"
                     /locus_tag="PXB44_001349"
     CDS             complement(6013..6546)
                     /gene="rplF"
                     /locus_tag="PXB44_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL5281826.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(6563..6955)
                     /gene="rpsH"
                     /locus_tag="PXB44_001350"
     CDS             complement(6563..6955)
                     /gene="rpsH"
                     /locus_tag="PXB44_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL5281827.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(6992..7297)
                     /gene="rpsN"
                     /locus_tag="PXB44_001351"
     CDS             complement(6992..7297)
                     /gene="rpsN"
                     /locus_tag="PXB44_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL5281828.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7309..7848)
                     /gene="rplE"
                     /locus_tag="PXB44_001352"
     CDS             complement(7309..7848)
                     /gene="rplE"
                     /locus_tag="PXB44_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL5281829.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(7866..8177)
                     /gene="rplX"
                     /locus_tag="PXB44_001353"
     CDS             complement(7866..8177)
                     /gene="rplX"
                     /locus_tag="PXB44_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL5281830.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(8188..8559)
                     /gene="rplN"
                     /locus_tag="PXB44_001354"
     CDS             complement(8188..8559)
                     /gene="rplN"
                     /locus_tag="PXB44_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL5281831.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8782..10617)
                     /gene="glmS"
                     /locus_tag="PXB44_001355"
     CDS             complement(8782..10617)
                     /gene="glmS"
                     /locus_tag="PXB44_001355"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL5281832.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(10665..11426)
                     /locus_tag="PXB44_001356"
     CDS             complement(10665..11426)
                     /locus_tag="PXB44_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL5281833.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(11583..11783)
                     /locus_tag="PXB44_001357"
     CDS             complement(11583..11783)
                     /locus_tag="PXB44_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5281834.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
BASE COUNT         3369 a         2675 c         2110 g         3637 t
ORIGIN      
        1 ataaaacccc ggtatttcta ccagggtttt aagctcaatt ttgattaaga ttattccgca
       61 gctgcttcag ttgcaacttc tgggcgatcc actaactcaa tgtaagccat tggagcatta
      121 tcacctgcac ggaaaccaca ttttaaaatg cgagtgtaac cacccgcacg ttgtgcaaaa
      181 cgtggaccta attcattgaa taatttagca actgtatcaa cgttgcgtgt acgggcaaaa
      241 gctaaacggc gatttgcaac gctgtctgct ttagctaaag taattaacgg ctcaactaca
      301 cggcgtaact ctttcgcttt tggtaaagtt gtcttgatga tttcgtgttc aacaagcgca
      361 cttgccatat tacggaacat cgcttggcga tggctgctgt tacggtttaa ttgacgtcca
      421 ctcttacgat ggcgcataac ctattccttc ttagaaaatc tataccgaaa aactagtctt
      481 ctgcaatact tgcaggtggc cagttttcaa ggcgcatacc gagtgataaa ccacgagaag
      541 caagaacatc cttaatctct gtaagagatt ttttacccaa gttcggggtt ttaagaagtt
      601 caacttctgt acgttgtact aaatcaccga tatagtgaat tgtctctgct ttcaaacagt
      661 tagcagaacg aactgtcagc tctaggtcat ctactggacg aagaagaata ggatcaaatt
      721 ccggtttttc ttctttcact tcaggttgac gaacatcacg caaatcaacg aatgcagcaa
      781 gttgttctgc taaaatcgtt gcagcacgac ggatagcttc ttcaggatct aacgttccgt
      841 tagtttccat ctcaataaca agtttatcta agtctgtacg ttgttcaaca cgagcagctt
      901 cgacattgta agcaatgcga tctacaggtg aaaaacgtgc atctactaat aaacgaccga
      961 tagggcggtc atcgttttga gtatgaagac gtgctgatgc aggaacataa cctcgaccac
     1021 gttgaacacg aatgcgcata ttaattgatg cattcttatc tgttaaatga cagattacgt
     1081 gatcaggatt cacaatttct acatcgccat cgtgtgtgat gtcggctgca gttacagggc
     1141 caattccaga tttatttagt gtcaaaataa tatcatcttt attttgcact tttaccgcta
     1201 gacctttaag gtttaataat acctcaagaa tatcttcctg aacgccttct ttgctgctgt
     1261 actcatgcag tacaccatca atttcaactt cagtaactgc acaacccggc atagatgaaa
     1321 gtaaaatgcg gcgaagtgcg ttacctaatg tatggccaaa accacgctct aacggctcta
     1381 atgtcacttt agcatgagtt gaactaactt gttcaatatt tactaagtgc ggctttaaaa
     1441 attctgtcac agaaccctgc attttttcct ctctttgcgg ttaagctaac tattatttag
     1501 agtaaagctc aacgatcaga tgttcattaa tatctgctga tagatcagaa cgctctggac
     1561 gacgtttaaa tacgccttcc attttagttg catcaacttc taaccaagtt ggtttttcac
     1621 gttgagttgc taattctaat gatgctttaa tacgtgcttg ttttttcgat ttttcacgaa
     1681 cagcaacaac atcatcaaca gaaacttgga atgaaggaat gtttactaca cgaccgttta
     1741 ctacgattga tttatggcta acaagctgac gtgcttctgc acgtgttgct gcaaaaccca
     1801 tacgataaac tacgttatct aaacgacctt ccaataatac tagtaagttt tcaccggtat
     1861 taccttttaa gcggttcgct tctgtataat aattacggaa ttgacgctct aagataccat
     1921 aaatacggcg aactttttgt ttttcacgta attgactacc atagtcagat aaacgtggct
     1981 tgcgagcacc atgttgacca ggcgctacat caagtctatt tctacatttt gattcaatcg
     2041 cacgaacgcc tgattttaag aataaatcag taccttcacg acggcttagc ttgagcttag
     2101 gacccaaata tcttgccatt ttctttctcc aattctttta acgtcaatta aacacgacgt
     2161 tttttcggtg gacgacaacc gttatgtgga atcggagtca catcagtaat attcgtgata
     2221 cggaaacctg ctgcatttaa tgcacggatt gttgactcac gacctggacc cggtccttta
     2281 accataactt ccaaattctt taaaccaaac tctttaacca tttctgcaca acgctctgca
     2341 gcaacttgag cagcgaatgg agtcgattta cgagaaccac ggaaacctga accacctgca
     2401 gttgcccaag ctagagcatt accttgacgg tcagtaatag taacgattgt gttattgaaa
     2461 gatgcgtgga tatgagctac gccatctgca atctgttttt ttacgcgctt acgtgcgcga
     2521 actggtgttt tagccattat ttatctactc cgactatttt ttgatcggtt tgcgtggacc
     2581 tttacgggta cgcgcattag tcttagtacg ttgaccacgt accggtaaac tacgacgatg
     2641 acgtaaacca cggtagcaac ctaagtctaa aagacgtttg atgcttaaag ttacttcacg
     2701 acgtaaatcc ccttcgacag taaatttacc aacttcttca cgcagttttt caatctgctc
     2761 ttcagacaat tctctgatct ttacatcttc agcaataccc gttgcagcac agatggcttt
     2821 tgcacgagtt ttaccgatac cgtaaattgc agttaatgcg attacagtgt gtttttgatc
     2881 aggaatgtta atgcctgcaa tacgggccac tatgcactcc tattatttaa gtttattgat
     2941 atactgatct tgaaaagccc gtttcaggat actcaaacag tatatcagga cgaatgagct
     3001 gagcagtata actgctcagc gggttctttg caagaaatat gcgtaaatta accttgacgt
     3061 tgtttgtgtt taggatcgct acaaattaca cgaaccacac cttcacgttt gattacttta
     3121 caattacggc acattctttt tactgaagca cgaactttca ttgcttatcc tttattaacg
     3181 gcctattaat ttagaccttt taagttagct ttcttcaaca cagaatcata ttgttgagac
     3241 attaaatgac tctgaacctg tgcgatgaaa tccataataa caaccactac aatcaataat
     3301 gatgtaccac ctaattggaa tggcaccttc cataacgacg taatgatgta aggaaccaaa
     3361 caaacaaaag caatatataa cgcaccaatt aaggttaaac gtgtcattac tttatcgata
     3421 taacgagatg tttgttcgcc tggtcggtaa cctggtacaa acgcacctga tttcttaaga
     3481 ttatccgctg tatcacgagg attatactgc attcctgtat aaaagaaagc aaagaaaatc
     3541 actgccattg tgaataatac aacatacagt ggttgacctg gctgtaatag ttgtgaaagg
     3601 ttaaataacc actcaaatcc ttcgctttga ccaaaccatt gagtaatgct tgccgggaat
     3661 aaaataatac ttgaagcaaa aattgccggt attacccctg ccatattcac ttttaatggt
     3721 aagtgagaag ttcttgcagg cgccatcatt cgaccttgtt ggcgacttgc atagtttaca
     3781 actattcttc tttgtccacg ttcaacaaaa acaacaaaat aagttactgc aaatgcaatt
     3841 attgcaacta gtagtaagac gataaatgat aactcgcctt gacgggcttg ttctattgtc
     3901 tgccctatcg ttgctggaag atctgcaaca atacctgtaa aaataatcaa tgagataccg
     3961 tttccgatac cacgctcagt gatttgttcg cctagccaca ttaggaacat tgttcctgtc
     4021 actaagctta ctacagaggt tacatagaat aatatacttg gattaggaac taatccatgt
     4081 accatattcg gtagggctag tgaaatacca atagattgaa taaatgctaa taacagtgtg
     4141 ccatagcgtg tatatttact gattttccga cgacctgctt caccttcttt ctttaattca
     4201 gctagaggtg ggtgaatcgc tgtcaataat tggataataa ttgacgctga aatataaggc
     4261 ataataccta gtgcaaatat agatgcacgg cttaaagcac caccagagaa catattaaac
     4321 atatcaatga tggtgccttg ctgttgtcga attaattcgg ataatacaga agcatcaatg
     4381 ccaggaacag gtataaaaga accgatacgg aaaacgatta acgcacctaa aacaaacaat
     4441 agtcttgatt taagctcgcc agtccctttt tgtgtactac cttggtaccc tggttgcttt
     4501 gccatcttta ttcctcgata gaaccgccag cagcttcaat agcagcttta gcgcctttag
     4561 ttacacgtaa gcctttaaca actactgctc tttcaatttt gcctgctaaa attactttag
     4621 cagataaaat atctttagta atgatgtttg ctgcttttaa tgtctctaaa gttacttcgt
     4681 tgccatctac tttagctaag tcgtttaaac gaatttcagc cacatggaat gattttaatg
     4741 aagtaaaacc aaattttggc aaacgacgat ataaaggcat ttgaccaccc tcgaaaccac
     4801 gacgaacacc gccgcctgta cgagattttt gacctttatg accgcgccca ccagttttac
     4861 ctaaaccaga accaatacca cgacctagac gttttgcact gtgcttagca ccttcagctg
     4921 gagaaagaga atttaaacgc attctattac tcctctactt taaccatata agacacttga
     4981 ttgatcatac cacgcactgc tggtgtatct tcaagttcta cagtatgacg aatatgacga
     5041 agacctaagc cttttaacgt tgctttatgt ttcggtaaac gagcgataga gctacgagtt
     5101 tgagttactt taatttttgc catatccaat taccccaaaa tttcttcaac tgttttgcca
     5161 cgttttgcag caaccatttc aggtgatttc atgttctcaa gtgcatcgat tgttgcacga
     5221 acaacgttaa ttgggttagt tgaaccatac gctttagaaa gaacgttacg aacacccgca
     5281 acttctaata ctgcacgcat tgcaccacca gcgatgatac ctgtaccttc acttgctggt
     5341 tgcataaata cgcgtgaacc tgtatgtgaa cctttaactg gatgttgtaa tgtaccttca
     5401 tttaaagcta cattaatcat attgcgacga gctttttcca tcgctttttg gattgctgct
     5461 ggaacttcac gtgctttacc gtgaccaaaa cctacacgac cgttaccatc gcccactact
     5521 gttaaagcag tgaaactcat aatacgacca ccttttacgg tttttgaaac acggttaacc
     5581 gcgattagct tttcctgcag ttcaccagct tgtttttcga tgtttgacat ctcaaattcc
     5641 tctattagaa ctgtagacca gcttcacgag cagcatctgc taatgattgc acacgaccgt
     5701 ggtatttaaa accagaacgg tcaaaagcaa ccgctttaat acctttggca attgcacgct
     5761 cagcaacgat tttacccact actgctgcgg cttctttatt gccggtatat ttaacttgct
     5821 ctttaattac tttttcaaca gttgaagctg ctgctagtac ttctgagcca ttaggtgcaa
     5881 tcacctgcgc atagatatgg cgaggtgtgc gatgcacaac taaacgtgtt gcaccttgct
     5941 ctctcattaa atgacgtgca cggcttgcac gacggatacg agctactttc ttatccatag
     6001 tgttacctta ctttactttt tcttcgcttc ttttgtacgc actacttcat cagagtaacg
     6061 tacaccttta cctttataag gttcaggttt gcgatatgca cgaatatctg ctgccacttg
     6121 accaattaac tgtttgtcag cacttttcag aataatttct gtttgtgatg gacattcacc
     6181 agttacgcct gctggcaacg tgtgttctac tgggtgagag aaacctaaac ttaaagcaac
     6241 agcattgcct ttcatttgtg ctctataacc aacacccact aattgcaatt tcttagtaaa
     6301 gccttcagta accccgatta ccatattatt tactagtgca cgagcagtac ctgcttgagc
     6361 atctgcatta gcgatacctt cacgtggagc gaaagttaat gcatctgctt cttgttttac
     6421 ttcaactgca ttgtgaattt cacgagataa ctcgccgttt ttacctttaa ctgttaatag
     6481 ctgaccgtta agttttacct caacaccggc aggaatatta acaggtgctt ttgcaacacg
     6541 agacattttt cctacctctc tattatgcta cgtaacagat aatttcaccg ccgaggcccg
     6601 cttgacgcgc agcacggtcg gtcattacac ctttagatgt agaaacaact gcgataccta
     6661 aaccacccat tacttttggt aattcgtctt tgcgtttata aatacgcaga cccggacggc
     6721 tcacacgttg gatactttct acaaccggtt tgttttggaa atattttaaa gtaatttcca
     6781 gttcaggttt aacaccttca acaactttaa cgctttcgat ataaccttct tcagctaaaa
     6841 catttgcaat agcaactttt aacttagatg aaggcatact gattgcaact ttgttcgcag
     6901 cttgaccgtt acgaatacgg gtcagcatat ctgcgattgg atcttgcatg ctcatgtatt
     6961 tactcccatg attccaaatt aaaagaggtt attaccagct tgccttctta agacccggaa
     7021 tttcaccgcg catagcagct tcacgaacct taatacggct taaaccaaac ttacgaagta
     7081 cgccatgtgg acgaccagtt tggcggcaac gattacgctg acggattggg ctagaatcac
     7141 gaggaagtgt ttgtaatttt aacactgctg cccaacggtc ttcatcagat gcattcacat
     7201 cagagatgat tttctttaat tcttcacgtt ttgcgtagaa tttatcagct aatttagcac
     7261 gttttacatc gcgtgcgatc attgattgtt ttgccacgat aacctacctt atttacggaa
     7321 tgggaaattg aaagcagcta ataaagcttg accttcttca tcagttttcg ctgaggtggt
     7381 gatagtaata tctaaaccac gaacacgatc tactttatcg tagtcgattt cagggaaaat
     7441 gatttgttca cgaacaccca tactgtaatt accacgacca tcgaatgact tagcgtttaa
     7501 accgcggaag tcacgaatac gtggaacagc gatagtaatc aatctttcaa agaattccca
     7561 catacgttca ccacgtaggg ttactttgca accgattgga tacccttgac ggattttaaa
     7621 gcctgcaaca gatttgcgag ctttagttac taaaggtttt tgaccgctga ttgctgctaa
     7681 atccgctact gcgttatcta acagtttttt gtcggtcaat gcttcaccca cacccatgtt
     7741 aagggtaatc ttttcgattc gtgggacttg catgacagat gagtagttga attttgcttt
     7801 taattcatta actactgtat ctctgtagta atcatgcagt ttcgccatca cattactcca
     7861 gttaattaaa ttgttttctc gttagatttg aagaaacgca cttttttgcc atcttcgaat
     7921 ctaaatccta cacggtcagc tttgttggtt tcagggttga agatcgcaac gtttgaaaca
     7981 tcgattgcag cttctttctt cactaaacca ccagctttgc ctaatgcagg aactggtttt
     8041 tcgtgtttag tgatgatgtt gataccctca acaaaaactt taccatttgg taacacttta
     8101 gttaccttac cacgtttgcc cttatcttta ccggtaagaa caattacttc atcgttttga
     8161 cggatttttg cagccattac attctcctta tagtacttct ggagctaaag agatgatttt
     8221 cataaacttc tcagaacgaa gttcacgagt caccggtcca aaaatacgag taccgattgg
     8281 ttgctcagtg ttattgttta aaattacaca agcattgcca tcgaaacgaa taactgcgcc
     8341 atctgggcga cgaacaccct tcttggtgcg cacaacaact gcttttaaca catcaccttt
     8401 tttcacttta ccgcgtggaa ttgcttcttt tacagtaact ttaatgatat cgccaatagc
     8461 agcgtaacga cggtgcgatc cacctagaac cttgatacac attacgctac gagcccctga
     8521 gttatcagca acatccagca tagtctgttc ttggatcata ttagtgctcc gttaaaataa
     8581 aaaaacaccc tttcgggacg aacctattta tacaaatata aatagggtgc aaaatatact
     8641 acaaaaacca ctagttaatc aagtgaattt ttaagctaat ttaatagttt aagtaagctt
     8701 atttataaat tgtttcaact atcaataaac tgtcctataa ctcatataaa aaccccaaga
     8761 tatcttgggg tttaactaac attactctac agtcaccgct tttgcaaggt tacgaggttg
     8821 gtcaacgtcc gttcctttga ttaaggcaat gtgatatgaa agtagttgta acggcacggt
     8881 gtagaaaatc ggtgcggtta cttcatcaac tttcggcatt acaatcgttt tgaagccttc
     8941 tgcttcttca aaacctgcat cgtgatcggc aaacacatat aactgaccgc ctcttgcacg
     9001 cacttcttca atattggatt tcactttctc aagcaagtca ttttccggtg ctaccaccac
     9061 taccggcata tcgctatcaa ttaatgctaa cgggccgtgt tttaactcgc ctgcggcata
     9121 agcttctgcg tgaatatatg aaatctcttt taatttcaat gcagattcca tcgcaaccgg
     9181 gtaatattct ccacgaccta agaacaatgt gtggtgttta tccgcaaaat cttccgataa
     9241 tttttcgatt tgtttatcga acactaacgc actttcaatt tgtgctggta agcgttgtaa
     9301 agattgcacg atatggtgtt cttgctcttc actcattctg ccttgtaagc gtccaatcgc
     9361 cacatttaat aacaataagc aagttaattg agtagtgaat gctttggttg atgctacacc
     9421 aatttcaaca ccagctttgg ttaagaatgc aaaatcggat tcacgcacta atgaagaact
     9481 tgccacatta cacaccgtca ttgccgccat atagccagat tctttcgcta aacgcagtgc
     9541 agctaacgta tctgcggttt cacctgattg tgaaagcgtg attaataagc tgttcgggcg
     9601 agtaacgaat ttacggtaac ggaattcgga ggcaatttcc acatcacagc tcacgcctgc
     9661 aatggattca aaccaataac gtgaaaccat accagcattg taagaggttc cacaagccac
     9721 aatttgcacg tgttctactt tcgataaaat ctctgccgca ttcggtgcaa tggctgaaat
     9781 atctacttta ccgtctttga tacgaccatc taaggtattc ataatcgcaa ccggctgctc
     9841 aaaaatctct ttttgcatat agtgacgata ttgtccttta tctgccgcgt ctgcctcaaa
     9901 attgccttcg tgaatttcac gctctgcctt gttgccttca cggtcataaa tatccactgt
     9961 gcgacgggta atttcggcta catcaccttc ttctagataa gcaaaacggc gagttacgct
    10021 taataatgca agtgggtctg aagcaaggaa gttctcgcct acaccgtaac caatcactaa
    10081 cgggctacct gagcgagcca caattaaatg ctccggctct tcttcattta ataccactgt
    10141 gccgtaagca ccacgcagtt gcaccacggt tttttgtacc gcttccaata atgaagaagc
    10201 agaacgcaat tcccattcaa ctaaatgagc aatgacttcg gtatcggttt gagattggaa
    10261 cacataacca cgctcttgta acactatttt taactcttcg tagttttcga taatcccgtt
    10321 atgaactact gcaattttgc cactgcggtg tgggtgagca ttagtttctg acggctcgcc
    10381 gtgcgttgcc cagcgagtgt gtgcgatccc agtgccgcct aatagtggct tagtgtctaa
    10441 cgcctcatct aaggctttga ctttaccgac acggcgtaca atttgcattg ttttatcatc
    10501 tgcatttaat actgctacac cagcagagtc atatccacgg tattctaagc gatgtaaacc
    10561 atcaaccaag atttctgcga tatcacgttg tgctactgca cctacaattc cacacataaa
    10621 tttgctttcc tatgtttaag tttaaaaatt catttgagct actgctattt tgcaataacc
    10681 acttcgacac ccattttttc gatttgttct ttcgtttcaa aacttaattg actgtcggta
    10741 ataattttgg taatattttg ccatttcaat tcaatattcg gcattttccg accgattttt
    10801 tgcgattcaa ttaacacaat cacttcacgg ctcacttctg ccatcacctg acttaagcca
    10861 actaattcat taaaggtagt cgtgcctcga gccaaatcta aaccgtccgc accaataaaa
    10921 agctgatcga aatcatatga acgcaatacc tgttctgcta ctttgccttg aaaggactca
    10981 gaacggctat cccaagtgcc gccggtcatc aacactgttg gttcgcattc gagggaagtc
    11041 aattccgtcg ctaaattcag cgaatttgtc atcaccacca agccggtttg gttcaatgct
    11101 tttaccaaag ctccggtcgt gctgccgctg tcaataatga tgcgattatg atcacgaata
    11161 caatcacgtg cggcttgagc aatcattttc ttttgaatcg aaagttgctc aataaacgct
    11221 tcttccatca tttccgatgg aatttttacc gccccaccat acttacgcaa taaaaaacca
    11281 ctttcttcca aagcggttaa atcttttcta atcgtaactt cagacgtctc aaagaaatga
    11341 gcgagttcat ctacactcac ttcatttttc tcttgtacca tttgaacaat taaatggcgg
    11401 cgttgttgcg tgttacgttt tgacattata atcttcactt aaatttcgtt tcaaaacttt
    11461 tgcattataa agaaagctcg cgaaaagaaa agcagaaaat gcaaaattta caaaatctat
    11521 acaaaataaa accgcttgta atattgattt acaagcggtt gatttcttga aattttttgc
    11581 aattatttga tttggctacc aatcacgcca ccaagtgcgg caccacctaa agttgcaccg
    11641 gtagattcac caatcgcgtg accagccaca ccgccaattg ctgcaccgat tgcagtatta
    11701 cgttgcgttt ttgtcatatt gttacagcca gacacagcaa ccattaatac cattgcacca
    11761 acaactttca aaatagattt cataataatc c
//
LOCUS       DANLUA010000072        11698 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLUA010000072 DANLUA010000000
VERSION     DANLUA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            591..1319
                     /gene="ttrB"
                     /locus_tag="PXB44_001358"
     CDS             591..1319
                     /gene="ttrB"
                     /locus_tag="PXB44_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL5281835.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVMVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            1321..2352
                     /gene="nrfD"
                     /locus_tag="PXB44_001359"
     CDS             1321..2352
                     /gene="nrfD"
                     /locus_tag="PXB44_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL5281836.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            2342..5437
                     /gene="ttrA"
                     /locus_tag="PXB44_001360"
     CDS             2342..5437
                     /gene="ttrA"
                     /locus_tag="PXB44_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL5281837.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5578..6423
                     /locus_tag="PXB44_001361"
     CDS             5578..6423
                     /locus_tag="PXB44_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5281838.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6536..7933)
                     /locus_tag="PXB44_001362"
     CDS             complement(6536..7933)
                     /locus_tag="PXB44_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5281839.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(8023..8646)
                     /gene="gmk"
                     /locus_tag="PXB44_001363"
     CDS             complement(8023..8646)
                     /gene="gmk"
                     /locus_tag="PXB44_001363"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5281840.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(8759..9232)
                     /gene="gpt"
                     /locus_tag="PXB44_001364"
     CDS             complement(8759..9232)
                     /gene="gpt"
                     /locus_tag="PXB44_001364"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5281841.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(9378..10787)
                     /locus_tag="PXB44_001365"
     CDS             complement(9378..10787)
                     /locus_tag="PXB44_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5281842.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            complement(10983..>11698)
                     /locus_tag="PXB44_001366"
     CDS             complement(10983..>11698)
                     /locus_tag="PXB44_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL5281843.1"
                     /translation="AERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEKEVDPIA
                     IIKAKGIDDTNYGLKLGNSKETIEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNY
                     IDKVDGTVTVKGLKTEKLPTEGTATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGR
                     VEGEFGNLIVLEEGTINNTQISSTVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKAN
                     LTGEVSNFGLAGTRGDIQK"
BASE COUNT         3178 a         2708 c         2661 g         3151 t
ORIGIN      
        1 aataatgagg ttttcaaaag atataaaaat aatgatgcac aaccacataa acttcataga
       61 atctgacaga tttgcggaat ggattcttga aaaggcttgt gttcgctcta aaacattaac
      121 cagcttttat ggcgacaagt ctgttataag ggccgttcca ccattggatt ggtcaggaaa
      181 atataaatat accaaaatag gatttagatt atgctacgat ttgtaggggg atatatgaag
      241 aaatacagta atgattctct tataaatagt attgttatat ctaaattaaa agcaggatgg
      301 aaaattagaa aaggaaagaa acataatata ttaattgccc caaattctag gagattagcg
      361 atacctagta ccccgagtga tattaatgcg tataaaaact ttagatctaa actaaaccat
      421 cttattgctt tttaattagt atcaatgccc tattatctac ttgtataggg catttccctt
      481 catccttgac ccaaatcaac tttccccttg atatgtggaa aaccacaata ccttgccgtt
      541 ccctctccgc cttagaatac ctttgttatt tttaaatttg tgggggaaat atggatttaa
      601 gcaaacgttc ctttttgaaa gagctttccg ccttgacggt gggggcgagt tttgtgccgt
      661 tgcaatcggc ccaggcgttt atgccggctt ggcgtgaggg cgatgagaac aaacgctatg
      721 cgatgctgat tgatttgcgt aaatgtatcg gctgtcaagc gtgtaccgtg agctgttcgg
      781 tggagaacgc cacgccgttg cacagtttcc gcaccacggt gcgtcaatat gaaatcacca
      841 acggcacgca ggtggtgaac aatgtggtgt tgcctcgttt gtgtaaccat tgcgatcaac
      901 cgccgtgtgt gccggtctgc ccggtgcagg caacctttca acgtaaagac ggtgtgatgg
      961 tgattaacaa tgagcagtgc atcggctgtg gttactgcgt gcaagcctgc ccgtatgatg
     1021 cccgttttat caatgaagag accaaaaccg ccgataaatg caccttctgc actcaccgtt
     1081 tggaggtagg tctgctgccg gcgtgcgtgg aaagttgcgt gggcggggca agggtgattg
     1141 gcgatttgaa cgacccgaaa agtcaaattt cccaacttta tcaagcccac aaagatgatt
     1201 tgaaagtcct gaaaccggag gccggcaccg tgccacacgt tttctatgtg gggctagacg
     1261 atgatgcctt tgtgagcaaa attgacgggc aaccaatgct ctggacgggg gaggcgtaaa
     1321 atgattcgtg aagtattagt ggaaccgcaa catatcgtct ggttgccgtg gattgtgcat
     1381 tatttcttct tcgtaggcgt ggcggcaacg gcagtgttta ccgccgtgct gtttgcaaaa
     1441 aaatcactga aaccgaccgc ttgcgagctg gcggcggtga cggtagcctt gattggctcg
     1501 attgtcgccc ctgtggcatt gactgccgac ctgcaccaac cgagccgtat tctgcatttc
     1561 tacaccgatt ttgcgtggcg atctccaatg gcgtggggag cgattatcct gcccctgttc
     1621 agcgtggcgg tagcgggtta ttttgtgctg gcattggctc accatacccg gccgaacctg
     1681 ccgaaatggt tagcgtggct acagttgccg atactgcaaa atcaaggctt gctttgggca
     1741 ttccgcctgt ttgccacact caccgctatc ggcattatcg gctacaccgt gcttgaaacc
     1801 taccaaaccg gcacaagaac cttatggcac agtgcgtggc tgctgccgat tatgctcttt
     1861 tctgcgtggg cggtggcatt ggggcttacc caagcggtct gcaaaatgct gttccacacc
     1921 caaaccaaca cggggctatg gacaaaactc tgcctgattt taaccgcact ttcgcttgtc
     1981 ggactagcat tcagtagtcc acaagccaca caagattttg cccagctctt caatggctca
     2041 atcaccgctt accttgtcgg cattttctgg ctaattgccc tcatttgcaa tttttctgcc
     2101 aaaaaccacc gcttgcaatg gctcggcgtg cttgccctca tcgctttcgg ctggctactc
     2161 cgctgggtac tggtgatcca agtccaaacc attgccaaaa ccaatgcctt gcaaaatcca
     2221 tatcatttcg attggacagc cgtcgatggt ggtttgggga ttgtctcgat ttttgggcta
     2281 acggtgctag taagtatcgt ggtatggcaa ctgattttaa taatttgtgg gggaagaaaa
     2341 ggtgaacaat aaacgcagaa atctgttgaa aggcgggctg gcacttggtg gggcggcggc
     2401 gtttggggcc gggtattcgc cgaaggtgtc ggagattgcc aaaggggtga tgcacggcac
     2461 atcgggcgag aaaacccgtg atgcaattca cggtaattcg ctcacgccgg agtatcaggt
     2521 aaaagacggc aaactgctca gcaatcctga gcaaattgtg tgcaatacac agtgtatggg
     2581 ctgttggacg cagtgtggca ttcgggcgag ggtggatttg aacaccaacc aagtgattcg
     2641 catttcgggc aatccgtatc acccgctttc ctccgacaaa accctgccgt tcggtatgcc
     2701 gattgccaag gcggaactgt tgctggcggg cgaaagtggc attgaaaatc gctccaccgc
     2761 ctgtgctaga ggggcggcgt tcttggaggg ggtgaacagc ccgtatcgga ttacccaacc
     2821 gttgaaacgg gtcggaaaac ggggcgaagg caagtggcag accatcagct ttgagcagtt
     2881 ggtggaggaa atcatcaatg gtggcaacct atttggtgaa ggcgaagtgg agggcttgaa
     2941 gacgatccgc aatctggtcg atccgattaa cccgaaacag cctgatttgg gaatgaaagc
     3001 aaaccagctt ttagttactt ttgccggccc cgaaggtcgt cagccgattt tgcaacgctt
     3061 tgcccaaaag agcttcggca cgatcaactt ttccaaccac ggggcttatt gtggcgctgc
     3121 gtaccgtgcc ggttcgggtg cctttatgaa agattttgac aaccaacaac acgccaagcc
     3181 ggactgggat cacgctgaat ttattctgtt tatcggcacg tctccggcac agtcgggcaa
     3241 tccgtttaag cgtcaggctc gccagcttgc caaacgccgc cctgatgaca atttcagcta
     3301 tgtggtggtt tcccctcgtt tggagatgac ctcctccctc gccacccaaa acaaccattg
     3361 ggtgccgatt aagcccgagg gcgatttggc gttggtaatg ggaatgttgc gttggattat
     3421 cgaaaacgag cgtttcaatg ctgactacct ctcccgccca agtctggcag cgatggaaaa
     3481 tgccggccac gtcagctatg ccaatgcgtc acatctgatt atttgcgaac cgaaccaccc
     3541 gaaattcggg caggcgttgc ggattagcga tctgcaagat gtgcaactcg accccgaaga
     3601 gaaagtggaa gaaaacctga ttgtggtaaa agatcaggca agcggcgaac tcatttcagc
     3661 aaaagattgc ttacaagcgg tcatttttgt cgaacaaatt gcaaaactgc ttgatggctc
     3721 ggaagtgttg gtgaaatcct caatgcagtt gctgaaagag gcgacacaag agaaaaccct
     3781 tgccgaatat agcgacattt gtggcgtgcc ggtgcaaacc attgaaaact tagcgaaaaa
     3841 attcacttcg cacggtcata aagccaatgc catcactcac ggcggcacga tgcacagcac
     3901 cggtttttat accagttggg cgattttgtt gctgaatgca atggtcggta acctcaacaa
     3961 aaaaggcgga atggcagtag ccgcaggcaa attcaaagat ttcggggacg ggcctcgcta
     4021 caatttagcg aacttcccga atgaagtgaa gccaaaaggc acgaaccttg cccgcagtaa
     4081 aaaggcgtac caaaattcaa gcgaatttaa gcagaaagtg acgaagggcg aaaatccgta
     4141 tccggcacaa ggggcgtggt atccgttcac cggcgggcaa actaccgaga tgtttacctc
     4201 aatggtgcag ggctatccat actttgcgaa agcgtggatc aaccatatgg cgaaccccgt
     4261 ttacggctta accgctatga acccgattgc gttggagaaa ttaaaagatc cgaaaattct
     4321 accgctcttt gtctctattg atgcttttat gaacgaaacc actgcactga cggactacat
     4381 cgtgccggac acccacaact ttgagagctg gggcttttca acggcgtggg gcggtgtgcc
     4441 tcaacgcacc agcaccgcac gccacccgat tgtgccgtcc aaaaacgcca aaactgccga
     4501 aggcgacacc atctgtatgg agaatttcgt gattgcggtg gcgaaaaaaa tgggcttgcc
     4561 gggctttggc gaaaatgcga ttgccgattt agaaggcaac cgctatccgc tccaccgtac
     4621 cgaagattac ttcctacggg cggcagcgaa cattgccttt gccggtgaga aacctgcacc
     4681 ggacgccagc gagcaagatc tgctgctcac cggcgtgcaa cggattatgc cgaaaattga
     4741 acaaacgcta aaagccgaag agcgattaaa agtggcgaat gtgtattgca aaggcggacg
     4801 ttttgcccca cacgaaagtg cgtggaaaga ggagaatatg gagttccagt ggaaaaactg
     4861 cctgcaaatt tggaacgaaa acgtgtataa agcccagcac cacagcaacg gtgaacacta
     4921 ttggggctgc cctcgttata tgtcgccacg ttttgcggac ggctccacgc tggagcagca
     4981 ctatccgcag tcggaatggg gctttaaact gatttcgttt aaatcgaaca ttatgagcag
     5041 tattaccgcc ccactgttgc gtctgctctc gatcaagcct gagggcttag tggcgatgaa
     5101 ccgtttagat gcggaagaaa aaggcattaa acacggcgat gtggtgaaac tcagcacccc
     5161 aaccgccggc ttgacggtgc agattgtggt gttggacggt atcgcccgag gcacaatcgg
     5221 catcgaacac ggctacggtc ataaacagat tggggcaagt tcctacacca ttgacggcgt
     5281 ggaaattcca gccaacccga tgatcgccaa agggattaac ctcaacgacc tcggcattat
     5341 cgaccacacc aaagcggtca aatccccgtg ggtagattgg gtctgtggct cagcggtgag
     5401 aaatggcatt ccggcgaagg tagagaaatt agcctagccg aacaagcggt tggttttgca
     5461 gaaaaatgtg caaaatcgac cgcttttatt gatgtaaggg acactgttcc ttatgcaaac
     5521 cagcattgca gagcgtaaaa aatcatactc tgcattttaa tttattttgg agaacctatg
     5581 acttttgtca aaaaaacagc actgagcctg attgcaacgg cacttttcgc tacatcaagt
     5641 tttgccgctg agcagagcca ataccaacat attcgtaatg caacggcgaa aattgaatat
     5701 gccggcaaga cgtttttggt tgatccgtat ttagcggaga aagatcgcta cttaccgccg
     5761 aatgcgaaaa aacgtaatcc gaccttggat atgactgtga gcattgatga agtgttaaat
     5821 ggcgtggatg cggtgatcgt aactcacact caccacgctc actgggacga tgtagcacaa
     5881 caacgtgtgc cgaaaaatct gccgattttt gtgcagaatg ccggtgatgc ccgtttaatc
     5941 cgttcacaag gctttaccga tgtgcgtgtg gtcggcaaag ataccccgtt tgaaggcgta
     6001 accttaaccc gaactcgtgg ccagcacggc accgatgcga tgtatgcgaa cccgaaaatt
     6061 gccgaaatgg tcggcgatgc gatggacgta gtgatgaaag cggaaggcaa accgaccgtc
     6121 tatttggtgg gagataccat ttggaaccac gaaatcgaac attcgttcaa cacttacaaa
     6181 ccagactttg tggtgctgaa tgccggctgt gccaaactac caaactttga aggcagcccg
     6241 attatgtgta ccgaagatgt ttccaaagca cacaaaatcg ctccacaagc gaaaatctta
     6301 actgtgcata tggacgccct gccacaaacc tcaatcagca gtgatcagat gcgtgattat
     6361 gtacaaaaag agggattaaa tcgtgtaact gtgccgaaaa acggtgatgt gattaagttc
     6421 tagtagctaa actaacatta ccaaaatggt ttaagtagca ataaatagcg aagcgggtta
     6481 agccatcaag ccttaaccca cttttaccaa ccatagtata tagttttaga tataattatc
     6541 ttctgtattt acctgccatt gcttggtaaa ccgcattctc gttggtcaaa atcgcatatt
     6601 ttgcatttaa aatcgccaac tgagaattaa gctcggtatt cagtgcggta atccactcac
     6661 ggaactccga caccccttgt tcataacggt ttttatagta tgaactgata cgtttatttt
     6721 tcgcattggt atcttgaagg atttttaacg agcttctcga ctgcgtataa ctgtaataat
     6781 aacggtcaat ttcatttaag gctgaggtaa ttttttgctc atagtttaaa cgggcagtca
     6841 cataatccgt ttcagaaatt tttacgttat ttttaacccg attccaatct aagaacggta
     6901 aatccaattt aattaagccg ttacccacag ggttatctgc cacattgccg atattggttg
     6961 cactaccggt aatagaacca ccgatggtta aggttgggaa ccagcttttc tcggttgcgg
     7021 ttaaggtttt aaatgcactt tgtaagcgtt gtaacgagcc tactacatca gggcgattag
     7081 caatggctga cactggcaca tttaaatcta ccccttgcaa tttcacgctc ataatattcg
     7141 ggtaacgaat gttaagcgga gtatttggct gaacatttaa caaattgcgt aatgtttgct
     7201 cggcaatttt ttggttggtt ttcagatcaa ttacactatt tctggcgttt aagaccgctt
     7261 gttgagcctg atcaaccgct aaactatcaa tcgcaccggc attcattttg ttagttaaaa
     7321 tacggctgat ttgctcataa tggcgaatgc tctcttcggt aatacgaatc gcatcacgat
     7381 agtaagctaa ctggtagtaa gtagtcacaa ccgcattaat aatagataaa cgggtagctt
     7441 ttaagtcttc aatggtggct ttatgcgtcc attctgccgc actggcggta tcggctaaac
     7501 gtcgccataa atccacagta taacttaaat taaatgcagc ttgagctgcg gttgttgaaa
     7561 caccggtcga aacattattt gaagaagccg aacctaaacc tttagccgca gaaacagaac
     7621 ctgaaccact aaaggttggc actaaatttg cccctactaa attagcgtta tacaaggctt
     7681 tattgactgc aattccgctt ttggctaaat caaggttatt tttgatggct aaatcaacca
     7741 cacggtttaa ttccgcatcc gtatagcctt gccaccattg ctcgttaatt tgatattgtt
     7801 tggtaatttc atcatagctt tgataggttt gctgtgcttg ttgcaaagtc ccgtcattgc
     7861 tcatattccc tgtggtacaa gccgttacgg ttagagccaa cgccacagaa aatgctaatt
     7921 ttgaaagttt cattacattt ccttttgaaa ataaagaggg gaaaatgggc gtattctacc
     7981 caaaaacaga ttgcctttcc agttaaatga acaactgttc atttaattat ttaacaaatc
     8041 agtaatcaaa tgctgatgac gaatttgttg cgaacctaag ctcaaacgct cggcaattaa
     8101 aatcgctttt agctctgcca atgcctgttg gaaatcatca ttaataatca cataatcgaa
     8161 ctcattgtaa tggttcattt ccgacaccgc ctctgccata cgtttggcaa tggtttcagc
     8221 ggaatcttgc ccacgcccga ttaaacgttt ttccaattct gtttttgacg gtggcaaaat
     8281 aaaaacggtt ttcacattcg gcactttttc ccgaatttgt ctagcccctt gccagtcaat
     8341 atccaaaaag acatcaatgc cctgctctag gctacgttca atcatcggca atgatgtgcc
     8401 gtaataattg ccaaacacct ccgcccattc taaaaaatac ccttgctcaa tcagctgctt
     8461 aaactcatcg tgcgaagtga aataataatg cacgccgtcc tgttctgccg gacgaggctg
     8521 acgggtagtg tgtgaaatcg aaagctgtac ttccgaacgg ggtaaatccg ccaacaaggc
     8581 gttaattaag gaagattttc ctgctccact tggggcagaa ataatataaa gattgcctaa
     8641 actcattatt tgtatatacc tatttgattt tgggaatagg gcgaatgatg attcgcccct
     8701 acatttcata ggaataaata aaaaataatg gacaactttc attgtccatt acacaaaatt
     8761 atctacgtgc caaaggctga acgaaggtaa tgcctaaatc ccaaggctgc tcaatccacg
     8821 tttcttgtgg aatatcaatc acataatcat caaccaacgg tgcaccgtgc ggtttcgcaa
     8881 acacggttac aaaacgagct ttcgggtaca ttctgcgaat ttcttttgcc gtgttgccgg
     8941 tatccactaa atcatctacc acaataaagc cttcgccatc ggtatcggct gcgtgtaaca
     9001 catttaactc accttggcta tcgtgatcgt agctggcaat gcacactgtt tctacgtggc
     9061 gaatacccaa ctcacgtgct aaaacggctg ccggaaacaa gccgccacgg ctcaccgcaa
     9121 taatcccttt ccactgtgtg gcaggtaata aattttctgc caacttgcga gcgtgcatat
     9181 ggaacatatc ccacgtcacg acatattttt catttgtgta tttttcactc atagaaacaa
     9241 cctaatataa aattgggggc taccccgata taaaaaattg cactaggata acccgaaaat
     9301 gcttttttcg ccatcatttc atttattttt ttgtgccggc agctacgcaa tcgtatgctt
     9361 agcacaaaaa accaacacta aaatagcttt tctaaagtga aaaagacttc cttgttttgg
     9421 cgagagtaaa aatcagcaat attgctgtca attttttgat aacggaaatt cagcttcggc
     9481 acaatgccct gccaatgcca agtgcggttc caaaccgcta aattggcttg atattccgtg
     9541 tctaaccgct tatagccaaa caaatagtgc ttgtcggcaa aatatcgctt gccaaagccg
     9601 atacttgcct gcgttgccca gcgttcgcct tgaaaaaccg cccccactcg agccagccac
     9661 ttattcgagc tttgtgcttt ttcccgtgca atttcacgca cgccgtctaa atttgcaaaa
     9721 aaccgcccat ttttgaccgc ttgccactgc acaccgccac tgagttggtg ctgatagctg
     9781 ttataacggc ttgcggttag ctcctcaaaa taccgttttt gcaaatgggt aaaactcccc
     9841 gacaccaacc attgcgaatt aaggtaacgc aaaccgttga gtgttgcccc aaattgaaca
     9901 ttataacggc tactccccag ccaattttgc tcaataaaag gcgaaaaagt cagccgataa
     9961 gcgacggtgc ggtagctata attcggcgaa acggatacgg attgctcacg gtaatctcgg
    10021 ttatcccaat agtaaatccc gctataattg agcgttaaac cgacaaaatg attgcctttc
    10081 acgttgtgca atttactcaa ccctaagcca taacgcacgc cttttgctga ttgtggcaaa
    10141 ctgtctgcat ttttgcggaa aatccgccca tttacattca caatcggcga agatgaggca
    10201 ttattcacgt tatctgtttg ggtatattgc agataaaaat cgggctgcca cgcctcattt
    10261 tcagcaattt ggacttgagt attgcgggca attttagcaa gttccggcgg taaatgggct
    10321 tgaatagcct taaattcctg ctctgccgca cgaaattgct gatcttctgc caaaatcaac
    10381 gccaaatcaa aacgagggta aaccaaactc gggggcttag ctaacagttg gcgatagtga
    10441 aaaactgcgg tttgatgatc ttttttcgca taggcaagtg cggcttgcgc atagtgccac
    10501 aacaacgggt caaaattttc agtttgcgga taacggctta aaagcggttc aagcgttgcc
    10561 caatcttggc tgagaatcgc ttggttaatt tgctgttcaa cattctgatt gactgaatac
    10621 gcaggctgat aagcggtcgg tttttgtgaa ttttttacaa cattttgctc aaatttaacc
    10681 gcttgcggct gacgctcaaa ggaaatcggg tcagtgttgt gttcaatcac cttttctgcc
    10741 catacaacgg ggctgaacag cgaaaataaa aggggatatt ttttcataaa gataaagaaa
    10801 attggtaggg gcggacctat atgttcgcct ctacgggatt tatcactctc tccccttgtg
    10861 ggagagggtt attagaagct gtaaattgaa atctaaacta ttgtgtctta tggtatctac
    10921 aagcaatatc aagacaaatg ccgtttaatt tttcgttaaa cggcattgaa cgttagaaaa
    10981 ggttattttt ggatatcgcc acgagtgccg gcaagtccaa aattactcac ttccccagta
    11041 agatttgctt ttgtctgaat ttcaccggca atttcctctg catttttacc gaaaaattcg
    11101 actgtatatt ttccagaaag gtttaaagga ttattgccat gtactgttga agaaatctga
    11161 gtattattaa tcgtcccctc ttctaatacg attaagtttc caaattctcc ctcaactcta
    11221 cctgaaccgg tacgcttaga gaaattaaca ttataggtta gatcaccacc attaaaacct
    11281 ttagctccat cgtggttaaa tgcttttcct gaatatgttg cagtaccctc tgttggtaat
    11341 ttctctgttt tcaatccttt aactgtaact gttccatcaa ctttatcaat ataattaccg
    11401 ttttcccagc catttctttg aactgcttgt gctgttacta cagaatatgg ctgattatat
    11461 aaataactct gtgttctgac tgtgacttta ccctcaatag tttctttact attaccaagt
    11521 tttaagccgt aatttgtatc atcaatacct tttgctttaa taatagctat aggatctact
    11581 tctttttctg ctgctaaacg ctccgcttcc gctttctctt tcgccgcttt ttccgccgct
    11641 aaacgttctg cttcggcttt ttctttcgct gctttttccg ccgctaaacg ctccgctt
//
LOCUS       DANLUA010000073        11582 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLUA010000073 DANLUA010000000
VERSION     DANLUA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            80..1057
                     /gene="manX"
                     /locus_tag="PXB44_001367"
     CDS             80..1057
                     /gene="manX"
                     /locus_tag="PXB44_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL5281844.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            1069..1869
                     /locus_tag="PXB44_001368"
     CDS             1069..1869
                     /locus_tag="PXB44_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL5281845.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            1883..2719
                     /locus_tag="PXB44_001369"
     CDS             1883..2719
                     /locus_tag="PXB44_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL5281846.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            2812..3297
                     /locus_tag="PXB44_001370"
     CDS             2812..3297
                     /locus_tag="PXB44_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL5281847.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(3383..4174)
                     /gene="tpiA"
                     /locus_tag="PXB44_001371"
     CDS             complement(3383..4174)
                     /gene="tpiA"
                     /locus_tag="PXB44_001371"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL5281848.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            4334..5248
                     /locus_tag="PXB44_001372"
     CDS             4334..5248
                     /locus_tag="PXB44_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL5281849.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHNRKIRVGSLAGNHFELLLRDVAES
                     EELIYRLQQLQAVGFPNYFTEQRFGRDGHNLTQALRWANGEISVKDRKKRSFYLSAAR
                     SEVFNLVVSQRINDCLTQKILENDYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTA
                     PLIGERSLELECNPAEKAIIEQHAQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGL
                     RLKFFLDSGSYATALVRELILLTEESEA"
     gene            5419..6207
                     /gene="surE"
                     /locus_tag="PXB44_001373"
     CDS             5419..6207
                     /gene="surE"
                     /locus_tag="PXB44_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL5281850.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            6194..6778
                     /locus_tag="PXB44_001374"
     CDS             6194..6778
                     /locus_tag="PXB44_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5281851.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            6796..7296
                     /locus_tag="PXB44_001375"
     CDS             6796..7296
                     /locus_tag="PXB44_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL5281852.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            7309..7509
                     /locus_tag="PXB44_001376"
     CDS             7309..7509
                     /locus_tag="PXB44_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281853.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            7528..8808
                     /locus_tag="PXB44_001377"
     CDS             7528..8808
                     /locus_tag="PXB44_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5281854.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            9049..9240
                     /locus_tag="PXB44_001378"
     CDS             9049..9240
                     /locus_tag="PXB44_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281855.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            9233..9799
                     /locus_tag="PXB44_001379"
     CDS             9233..9799
                     /locus_tag="PXB44_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL5281856.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(9846..10076)
                     /gene="acpP"
                     /locus_tag="PXB44_001380"
     CDS             complement(9846..10076)
                     /gene="acpP"
                     /locus_tag="PXB44_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL5281857.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(10263..10940)
                     /gene="rpe"
                     /locus_tag="PXB44_001381"
     CDS             complement(10263..10940)
                     /gene="rpe"
                     /locus_tag="PXB44_001381"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL5281858.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
     gene            11134..11406
                     /locus_tag="PXB44_001382"
     CDS             11134..11406
                     /locus_tag="PXB44_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL5281859.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            11415..11540
                     /gene="ykgO"
                     /locus_tag="PXB44_001383"
     CDS             11415..11540
                     /gene="ykgO"
                     /locus_tag="PXB44_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL5281860.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
BASE COUNT         3436 a         2384 c         2404 g         3358 t
ORIGIN      
        1 atacattgtg tgaaataatt atcatattaa aatctcgccc cagtaaataa acaacttaaa
       61 aaaccgcaag gagtaacaaa tgagcattgc tattatcata gcaacccatg gtgttgctgc
      121 agagcaactt ctcaaaacaa ctgagatgtt gattggtgaa caagaagatg ttgcttacat
      181 tgactttgtt cccggcgaaa atgcagaaac gattatgggc aaatatcaag cattggttga
      241 aggtccacta tcacactgtg atcaagtgct gtttttggtg gatatgtggg gaggaagccc
      301 attcaacgcc gcaaaccgat tccaagatgg aaaatcgggt atggatgttg ttgcgggtgt
      361 gaatattcct atgttagtga acacctgtat ggctcgtgat gatgtggagt cgttggaaga
      421 attggtggaa gtcgcattag aagccggctc aaccgggatt cgttcactta atcaccaacc
      481 tgaagtggct gaggtgcaaa ccgctactcc tgcacaagaa actgcagctg ttcaacagcc
      541 tgaaattgtt gatgacggac gtcgtatgac cattgccctt gcccgtattg atgaccgctt
      601 gattcacggt caagttgcga cggtttggac aaaagaaagt aaagtcaatc gcattattgt
      661 ggtaaacgaa ggtgtagcaa aagatagtgt acgaactact atgctaaaaa gtgttgctcc
      721 tcttggcgta acggctcacg ttgttgatgt acctaaaatg attcgtgtct ataacaaccc
      781 tgaatatgcc gatgatcgtg taatgttact ctttaccaac ccaagtgatg tcttagcctt
      841 aattgaaggc ggtgtaccac tcaatagcat taacgtgggc ggtatttcgc ataaagaagg
      901 gaaacaaatg gttagccatt ctgtggcggt tgatcaaaat gatattgatg ccttcaaagc
      961 aattgatgcg aaaggcgttg aattagaagt acgcaaagtc tcaagcgata gccgtcagta
     1021 tatgatggat ttattgaaaa aacataactt aatctaaagg aaattactat ggaactttca
     1081 gcattacaaa ttgtgctttt attcctcgtt gcctgtatct caggtatggg ctcaattctt
     1141 gatgaatggc aaacccaccg tccgttaatt gcctgtacga tgataggctt agtgcttggt
     1201 gatgtgacta ccggcgttat tgttggtggc tcattagagt tattagcttt aggttggatg
     1261 aacatcggtg ccgctgttgc accagatgcg gcattagcct ctattgtctc aaccatttta
     1321 gtcattgtag gtcgtcaaga tatttcaact ggtatcgcca tcgctattcc gcttgcagct
     1381 gccggtcaag tattaaccta tgttgtgcgt gcaatcaccg taggtttcca acacgcagca
     1441 gataaatcaa ttgaagacgg taacttaagc cgtttagact ggttacatcg tggtgcttta
     1501 ttgcttcaag caatgcgtat tgccattccg gcattaattg tggcattaac agcaggtaca
     1561 gatgcggttc aaacaatgtt aaatgccatt cctgaagtgg ttacaaccgg gctgaaaatt
     1621 gccggtggct tcatcgccgt ggtgggttat gcgatggtga tcaatatgat gcgtgcaggt
     1681 catttaatgc cgttcttctt cgcaggcttt gtaattgcag gctttaccga tttcaatctt
     1741 gttgcccttg gggtattagg tacggttatt gcaatggttt atatccatat gcaccctaaa
     1801 tataacaaaa gccaacaggt agtggtggca gctgcaccaa aaaatgattt agacaaccgt
     1861 ttagactaag agggcattta aaatgacaac tgaaatcaaa aaagtaacaa aaagcgattt
     1921 gaatgccgtt gtacgtcgtt caaacttatt ccaaggttcg tggaactttg agcgtatgca
     1981 ggctcttggt tttgcgtatg cgatggtacc ggttatcaaa cgtttatatc cggatccaaa
     2041 tggcccgggt cgcaaagaag caattaaacg ccattctgag ttctttaaca cccagccatt
     2101 cgtcggtgca ccgattttag gggtaaccat tgctatggaa gaagaacgtg ccaacggtaa
     2161 agaaatcgac aatgccgcaa ttaacggtat taaagttggt ttaatgggac cattagccgg
     2221 tgtgggtgac ccaattttct ggggaactgt tcgccctgta tttgccgcat taggagcagg
     2281 cttagcctta aaaggtgagc ttttaggccc aatcctgttt ttcgccttat ttaacatagt
     2341 gcgtttagcc gcacgctatt atggcgtagt gtatggctat aaaaaagggt tagatgttgt
     2401 acaagatatg agcggcggtt tattgcaaaa attaaccgaa ggtgcttcaa tcttaggtct
     2461 gtttattatg ggggcattgg tacaaaaatg gacgagcatt aacgttccgc ttgtgctttc
     2521 aaccattcaa aaacaagacg gtaccatcga agttaccaca gtgcaaagca ttctcgacag
     2581 cttaatgccg ggcctactcc cattattatt aacctttgct tgtatgtggt tacttcgtaa
     2641 tcgagtaaat gctatctgga ttatcctcgg tttctttgtt atcggtatcc tcggcgctgc
     2701 aaccggtgca ttagcttaat tgcaaaattt tctgtgaaaa cgaccgcttg aacttgaaca
     2761 ttcaagcggt tttttatacg ctttagccca aataaattca cttgcttaat tatgaccaat
     2821 accatcctta ttctctttat tttacttggc ttagtattcg ccatttatga ccaagttttt
     2881 atgcacaaac tcaaaggcac aaccttactg gaaatccgtt taaaaaagca aaaaacaatt
     2941 gatacttggt tattgattgg tttaatcgtc ttgtcaatta gctatggcgt acaaaatcaa
     3001 atccagcctt ttacacttta cctgctggcg acttgcatta ttctgtcggt ctatctcgcc
     3061 tttttccgtt cccctcgctt gttgttaaaa cagcacggtt ttttctttgc gaatgtcttt
     3121 tttaattacg acaagattta tcaagtcaat attgcagaag gaaaagcaga cgaaaaaatt
     3181 ttggtaattg atttacatag cggaagaagg ctgcttgcct atgttgaaaa tgcggaagat
     3241 cttcaaccgg tagtggattt ttttggcggt tataaaaagg aaagtgagca gaaatagttt
     3301 gcgaacaaaa cacaagcggt catttttccc tgaaattttg caaaaaactg aggaaaaatg
     3361 accgcttgta aaatcggtta aattaggctt tagcagccgc tgccgcttta acgatcaccg
     3421 caaaagcagg cgctttaagt gaaacaccgc ctactaatgc accgtcaata tccggttgag
     3481 tgaataactc tgctgcattg gcatcgttta cagagccacc gtattgaatg atcacttggt
     3541 cagccactgc ttgcgattta gctgcaatat ggtcacgaat aaatttatgc actgcttgtg
     3601 cttgtgccgg agtcgctgat ttgccggtac cgattgccca aattggttcg taagcaataa
     3661 ctgcaccgtt gaatgcttcc acaccgtgcg cgttgatgac cgcatcaatt tgtcttgcac
     3721 acacttcttc agttttacct gcttcatttt cggcttcaga ttcaccgata cataatacag
     3781 gcactaagcc ggcatctttt aatactttga atttttgagc aataaattca tcgctttctt
     3841 tgtggtaagt acggcgttct gaatgcccga taataatata ttttgcccca aactctttta
     3901 acatttcagg tgaaatatca ccggtgaatg ccccttttac gttcacatca acgttttgtg
     3961 aaccaagtgc gattaagcct ttatcactgc aaccacagcc acaaccggca agtgatgctt
     4021 cagcgtgtgc caaatacatc accggtggag cgattgctac atcacagcct tcaacgccat
     4081 gtaattctgc ttttaaatct gcaattaagg ttttagtaaa cgctttatca ccgtttaatt
     4141 tccagttacc cattacaagt ggacgacgtg ccattttgtt tctcctatat gaagttgaac
     4201 ttaaatataa acgttgcaaa ctataccaaa ttttaatgtc gttttgttgt ttttattttg
     4261 ctttttctga tctagatcga aaggtttaac gtataatgcc gaaatcttat tttttatgtt
     4321 attcgagaag tgaatgaaac tgaattattt atactctcct cctcaagctg ttgggcgact
     4381 aaaagccgaa tatgcagact ttattgtgcg tgaagaactg ggctacgaac tagccggcga
     4441 aggggaattt gtggcggtta aaatccgcaa aaccaacgcc agcacgctgt ttgtggggga
     4501 aaagttagcc aaatttgtcg gtatttccga gcgtgaaatg agttatgcag gcttaaagga
     4561 ccgccacaac cgcaaaattc gtgttggttc gctcgccggc aatcattttg aattattgct
     4621 acgtgatgta gcagaaagcg aagagttgat ttaccgtttg caacagttgc aagcggtcgg
     4681 atttcccaat tattttaccg aacaacgttt cgggcgagac ggacataacc tgactcaagc
     4741 cttgcgttgg gcaaacgggg aaattagcgt gaaagatcgc aaaaaacgca gtttctacct
     4801 ttccgctgcc cgctcggaag tgtttaattt agtggtttca cagcggatta atgactgctt
     4861 aacccaaaaa atcttggaga atgattacgt tcaacttgcc ggctctaaca gctttttctg
     4921 cgtgaaatcg gacgaaattg ctgaaactca agcccgttta gcacagcaag atctacttct
     4981 caccgctccg ctgataggcg aaagatcgct tgaattagaa tgcaatcctg ctgaaaaagc
     5041 catcatagag caacacgctc aattggttgc cttaatgcaa aaagagcgta tggcagccgc
     5101 tcggcgagca atgctttgta agccacaaaa tctggaatgg caatttgaac aagaagggct
     5161 acggttaaaa ttctttttag attcaggcag ttatgccact gcactggtgc gagagttaat
     5221 tctgcttacc gaagagagcg aagcttagtc cataacacat aacaagcggt aagattttaa
     5281 tcattttttg caatttgcaa aactttaaca aaatcttacc gctttttgca cttttagact
     5341 aaaaaaactg tcgaaaaatt gttagaattg cgatctttgt atttagagtc tatcaagatg
     5401 taagatctaa aggaaattat gcgaatttta attagtaatg acgatggttt tcacgctgtc
     5461 ggcattcaaa ctttagccaa agccttgcga gatgcaggcc atatcgtcac cattattgca
     5521 ccggatcgta accgcagtgc agcttcaagc tgcctaacct tagtagatcc acttcgggtg
     5581 catcgttttg atgaatataa ttatgcggtg atcgccggca cacctgccga ttgcgtgcat
     5641 ttagccttaa acggcttgtt tgacggcaaa tttgcagaaa aatttgattt ggtggtctcc
     5701 ggcattaatc acggggcaaa tttaggcgat gatgtggttt actccggtac ggtagcggca
     5761 gcattggaag gccgccactt gccattacca agtattgcag tatcactggt tgggcgtaaa
     5821 cactcttcgt ttttagacgg tgaaaatcat tttggaacgg cagcacaggt agtgttagat
     5881 ttattggaaa aaatggaaac gcaacgtttt ccatcaaacc acgttctcaa tgttaatgtg
     5941 ccgaatttac cttattcaga gctaaaaggc acaatgatca cccgcttagg tgaacgttca
     6001 cccgctgcgg aaattgtgaa gcaaaaagat cctcgtaatg ctgatgtcta ttggattggt
     6061 gtaacaggtg aaccactgga tgaaagtttt ggtactgatt tttatgcatt aaaccgagat
     6121 tgcgtttcta tcacaccaat tcaagtggat atgaccgctc ataaaacgat agcaacctta
     6181 aaaggcattt ttaatgaaac tgtttgaaac aatttatgac aaaacaatgc agtggtctaa
     6241 gcaccgcttc gctgcctttt ggttgagttt tgtcagtttt atcgaggcga tctttttccc
     6301 gattccgccg gatgtgatgt tgattccgat gtcgatggca aaaccgcaaa atgcgtttaa
     6361 atatgcagtt tacactaccc ttgcttcggt tgccggtggt gttattggtt attttattgg
     6421 cttatatgct accgattggg tgcaaggcgt catcgcaaat tggggaatgc aagcaaactt
     6481 tgataaagca aaattgtggt ttgaaacttg gggtgtcgcg gtagtatttt tagccggttt
     6541 ttcgccgatt ccctataagg tatttaccat ctgtgccggt gtaatgcaga tggcgttctt
     6601 cccgtttgtg attactgctg cgatttctcg ctttgcccgt tttattttgg tagcaaaact
     6661 ctctgcttgg ggtggcaaaa aatatgaaca aaaaattcgc cgctcaattg aattaattgg
     6721 ttggggaaca atcgttttag ccgttgttgc ctatataatt tatacattaa tgaaataaat
     6781 tttaaggata taaaaatgaa aaaatcactt tttgttttat ctctgatttc ttttgcttta
     6841 gtgggttgtt caagcaattc gggtgaatcc acttctgcgg tagaaacaac agaagcaaat
     6901 gcaacttggc aatcagcaga tagcattcaa actgcaccaa tgcctgcctc gatgaatcaa
     6961 cctattgcca ctcagccaac ctatcagcag ccgacttact ctgcaccaca accaatttcg
     7021 gcagcaccag caccaatgcc tgtttcaagt ggtagtgata gctctcaagt tgaaacagta
     7081 gggaactgcc aagtggtgcg tgatgcgaat aatgctccta tctattcaca aattaccaaa
     7141 ggctgctaca cagaaagtca ctatacggta ggtaaaagtg atacgcttta tttaatcggt
     7201 tatctaaccg gcaccaatgc caatcaaatt gcgcagctaa atggtttaaa taccggctct
     7261 ccactgaaag tcgggcaagt cttacgtgtt cgttaatagg cagagaaaat gaacagaacc
     7321 cttttagcat ttgcaatttc ttgtgcattt ttgaccgctt gtagctcgcc tactcagcca
     7381 caagttgaag atccaaatat gttacctgca ggcacattac aaccgattga aggctcaggc
     7441 gcaagccaag acagttacag ttggccgtct gatattcaat caacgtcaat gcctgacagt
     7501 atgaaataat tttaggacaa tcatttaatg aaaaaacaat ttattcttct ccctttagtc
     7561 tctgctgttt tggcagcctg ttcttcaaac aatccagcac cggtggtgaa tgcttccggt
     7621 aataacgaat taagcccggg cgtgatgcag cctgttgatg tgagtgcagg tgcaattaat
     7681 tcaagcggtg gctggcagtc tgatattcaa tctgcaccaa tgccaagctc gatgaatacc
     7741 actccggtgc aaacaatgcc ccagccggtt tcagtgccac aacctgtggc gactgtaccg
     7801 cagccaatta ccaatccggt gattaccgaa caaccaccac agccgacaac cacgaccaaa
     7861 atcgtgaaaa aaacgaaaac ggtcgagaaa aaagtggatc aaaattttga gatcccgcgt
     7921 gatgccaaca atgccccaat gtacaaccaa attcaaaaag ggttctacga tggctctacc
     7981 tataccgtac gcaaaggcga cacaatgttt ttgattgcct atattgtggg taaagatgta
     8041 aaagaaatcg ccgctcttaa taatatgagc gaaccttacc aattaagcgt agggcaaaaa
     8101 ctcaaaaccg gcaaatctgc aacggaaact attaccgtgg aagaaaaagt caccgtaccg
     8161 gttgaaccac aaattaccta ccaacaaggt gcaaacggca caacctatgc ttctggcggt
     8221 aatattactg ggccggtaaa agcaggcaca gccaacgctg atgtcacgcc ggttaataac
     8281 ggtattaaag caacaactgc aactgctccg gcagtaactg cttctgtcgg tacagtaaat
     8341 gcaacagagg accgtaccgc ctccaccatt cgtgctatag atggcacagc aagtggtgcc
     8401 gtacaaagta gtaatactgt ttctgcacca gcttcatcaa ttaaatggca atggccaacc
     8461 agcgggcgtg taatttcagg cttctcctca gcagaaggag gtaataaagg cattgatatt
     8521 gccggcaata aaggacaaga tgtacgtgct gccgccgcag gtaaagtggt atatgccggt
     8581 aatgccttgc agggttacgg taacttaatc atcatcaaac ataccgatga tttcttaagt
     8641 gcttatgcac acaacaatac tattgaagtt gatgagcaag aaacggtgaa agccggtcag
     8701 aaaatcgcga ctttaggtag tacaggaacc aatacgaata aattacattt tgaaattcgt
     8761 tacaaaggaa aatctgtcga tccggctcgt tatttaccaa gaaaataaga gtaacttttt
     8821 agcataatcc gaccgcttgt gtactacaag cggtcaattt ttaggaaaat tatgcaaatg
     8881 aaaaaatata tcgcacacat gataagttcg ttactgctga tagcttgttc ggactaccga
     8941 gcaacaccaa ctagcccaag cgatgcttac acttgctctc cttcttttcg ctactctttc
     9001 ttaaaaaaaa cgtgcatacc tttattcagt atagccgaca ttcaattatt ggatccaacc
     9061 gagccttcac atcgcattta tattttgtta tctgaagatc gatttcaagc cgaagtcttc
     9121 tcaacgaagc tctctgaatc ttctatgtta ttggaggcgg ttaaaggagg ctatatttca
     9181 aaagacggaa aagttcgttt actcaaaaaa gctgaaggtt ggaaaattta ctatgagtga
     9241 gactaaaata cgttgtgatt ggtgtaaaag cagtgaaatt tatatgaaat atcacgacga
     9301 agaatgggga aaaccggagt ttgacagcct caaactgttt gagaaaatct gccttgaagg
     9361 gcaacaagcc ggactttctt ggattacggt gttgaaaaaa cgggaagcct accgccaagc
     9421 attccatcag tttaatcctg aaaaaattgc caaaatgggt gaagaagaaa ttgatttgtt
     9481 aatgcaaaac actagcttaa tccaccaccg agcaaagtta gaagccatta taaaaaatgc
     9541 gaaagcttat atcacaatgc agcaaaatgg cgaagacttc agtcggttta tttggtcttt
     9601 tgtgaattat cagccgcaaa ttaatgatgt accaacactt gaagctgttc ctgctcatac
     9661 agaaacctca aaagcaatgt caaaagcgtt gaaaaaacgt gggtttgtgt ttgtaggcga
     9721 gactacttgc tatgctttta tgcagtcaat ggggctggtt aatgaccatt tgaataattg
     9781 cgtgttcaaa cataaataaa cacaaaaaat taggcgacca tttggtcgcc taacattaat
     9841 tcagattatt gattgttttg aacgtaatca attgctgatt gaactgtagt aattttttca
     9901 gcttcttcat ctggaatttc gatatcgaat tcttcttcta aagccattac taattcaaca
     9961 gtgtctaaag aatctgcacc taaatcttca atgaatgaag cttctggttt tacatcttct
    10021 gctttcgcgc caagttgatc aacaatgatt ttttttacgc gttcttcaat gctcattttg
    10081 tttttcctat agtgaatatc gcataagtgc gagagttaaa tagtgtaatc gaaataagtt
    10141 taaatacaac cttttataaa aaggtcaaac cagactattc ctatacacca gaatatatct
    10201 gtttttccat tctatcatta tgtttgtgtt taatctatag agataaatca aatttcattt
    10261 gattacccta ctttccgtaa ttcatctcgc atttgtgcaa tcactgcttg ataatctggc
    10321 tggtcaaaaa tcgcagaacc tgccacaaac atatctgctc ctgcggctgc aatgtcggca
    10381 atgttattta ttttcacacc accatcaacc tctaagcgaa tgttataacc gctctcatca
    10441 atacgacgac ggacttcttt caatttatct aaggtggacg gaataaatga ttgtccgcca
    10501 aagcccggat tcacagacat caataaaacc acgtcaactt tatccatcac ataatctaaa
    10561 tagctcaatg gcgttgccgg gttaaacacc aagcctgatt tacaacctaa atcacggata
    10621 agctgtaaat ttcgatctat atgttccgtt gcttcagggt ggaatgtaat ataatccgct
    10681 ccggctttgg caaaatccgg aatcaaacgc tcaaccggtt ttaccattaa atgcacatca
    10741 atcgggcatt gaatgccgta atcacgtaag gctttgcaga tcgccggccc gaaggtcaaa
    10801 ttcggcacgt agtggttgtc cattacatca aagtgaatta agtctgcacc ggcttttaac
    10861 acctctgcca catcatcacc caagcgagct aaatcggcag ataaaataga cggtgcaatt
    10921 aaataaggat tcttactcat atttcctcac tatcaaaaaa taaatctacg ttagattacc
    10981 ataattttaa cagcgaaaag agtctctctt cctaaaaatc cgttttttta caaaacacca
    11041 taaaaaagtt atggaattgt atttaaagtt cgtatattat gtatcccata ttttaattat
    11101 ggttaatgac cttatctaaa ttctggagaa aatatgaaaa aagggattca tcctgaaaat
    11161 tatcgtgaag tgttgttcta tgatgcctca gtgcaacaag gctgggttat ccgttcttgt
    11221 gcggcaacca acaaaacaat ggtatgggaa gatggcaaag aatacccact ttacacctta
    11281 gatacctcat cggcgtctca ccctgtttac acaggtaaac gccgtgaagt gaatacagaa
    11341 ggtcgtgcaa gtcaatttaa agatagattt aaaggctttg cttcaacgct ttcaaatcaa
    11401 aaatagagga taaaatgaaa atcttaaact cacttaaaac cgcaaaatca cgccacccgg
    11461 attgccaaat tgtcagacgc aaaggcaaac tttatgtgat ttgcaaaacc aacccgagat
    11521 ttaaagctcg tcagcgttaa aaaccagcac cttaataagc taatttatta aggtgttttt
    11581 ta
//
LOCUS       DANLUA010000074        11404 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLUA010000074 DANLUA010000000
VERSION     DANLUA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(6..491)
                     /locus_tag="PXB44_001384"
     CDS             complement(6..491)
                     /locus_tag="PXB44_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281861.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAGIALWTVYSHLDTKVEARFSKIDDK
                     FVQVETNIKNLDIRLTKVESRLDNVEQRLDNVEQRLGNMEKKIDGVDNKLDLLLQQFK
                     R"
     gene            complement(496..876)
                     /locus_tag="PXB44_001385"
     CDS             complement(496..876)
                     /locus_tag="PXB44_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281862.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            complement(927..1586)
                     /locus_tag="PXB44_001386"
     CDS             complement(927..1586)
                     /locus_tag="PXB44_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL5281863.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
     gene            1716..1922
                     /locus_tag="PXB44_001387"
     CDS             1716..1922
                     /locus_tag="PXB44_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5281864.1"
                     /translation="MNKIAEIRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVA
                     QKIVEGLNELGANVRIDDVFPVGN"
     gene            1943..2203
                     /locus_tag="PXB44_001388"
     CDS             1943..2203
                     /locus_tag="PXB44_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281865.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            2367..2537
                     /locus_tag="PXB44_001389"
     CDS             2367..2537
                     /locus_tag="PXB44_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281866.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            2512..3381
                     /locus_tag="PXB44_001390"
     CDS             2512..3381
                     /locus_tag="PXB44_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5281867.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            3378..4739
                     /locus_tag="PXB44_001391"
     CDS             3378..4739
                     /locus_tag="PXB44_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL5281868.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            4736..5272
                     /locus_tag="PXB44_001392"
     CDS             4736..5272
                     /locus_tag="PXB44_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL5281869.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            5363..5578
                     /locus_tag="PXB44_001393"
     CDS             5363..5578
                     /locus_tag="PXB44_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281870.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            5562..6068
                     /locus_tag="PXB44_001394"
     CDS             5562..6068
                     /locus_tag="PXB44_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL5281871.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
     gene            6118..6330
                     /locus_tag="PXB44_001395"
     CDS             6118..6330
                     /locus_tag="PXB44_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281872.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLFFLVILTVRYVGYLLIYPLAFFAA
                     VGSFIVFCQWTYQEWLYYFKRGFAPI"
     gene            6376..6570
                     /locus_tag="PXB44_001396"
     CDS             6376..6570
                     /locus_tag="PXB44_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281873.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
     gene            6698..7267
                     /locus_tag="PXB44_001397"
     CDS             6698..7267
                     /locus_tag="PXB44_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL5281874.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            7257..7730
                     /locus_tag="PXB44_001398"
     CDS             7257..7730
                     /locus_tag="PXB44_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281875.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTDKLKKYQFSP"
     gene            7958..8033
                     /locus_tag="PXB44_001399"
     tRNA            7958..8033
                     /locus_tag="PXB44_001399"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7991..7993,aa:Trp,seq:cca)
     gene            8050..8235
                     /locus_tag="PXB44_001400"
     CDS             8050..8235
                     /locus_tag="PXB44_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281876.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            8261..8431
                     /locus_tag="PXB44_001401"
     CDS             8261..8431
                     /locus_tag="PXB44_001401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281877.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            8574..8819
                     /locus_tag="PXB44_001402"
     CDS             8574..8819
                     /locus_tag="PXB44_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281878.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            8812..9381
                     /locus_tag="PXB44_001403"
     CDS             8812..9381
                     /locus_tag="PXB44_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5281879.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            9354..9704
                     /locus_tag="PXB44_001404"
     CDS             9354..9704
                     /locus_tag="PXB44_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5281880.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            9709..9882
                     /locus_tag="PXB44_001405"
     CDS             9709..9882
                     /locus_tag="PXB44_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281881.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            10274..10798
                     /locus_tag="PXB44_001406"
     CDS             10274..10798
                     /locus_tag="PXB44_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5281882.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            10782..>11404
                     /locus_tag="PXB44_001407"
     CDS             10782..>11404
                     /locus_tag="PXB44_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL5281883.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMRGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDA"
BASE COUNT         3653 a         1956 c         2581 g         3214 t
ORIGIN      
        1 ttattttatc ttttgaattg ttgtagtaac agatcgagtt tattatcaac cccatctatt
       61 ttcttctcca tattgcctag tcgctgctca acattgtcta gtcgttgttc gacattatcc
      121 aatctagatt caactttagt gaggcgaata tcaagatttt tgatattggt ctctacttgg
      181 acaaacttat catcaatttt cgagaatcga gcttcaactt tggtgtcaag gtgtgaatat
      241 actgtccata atgcaattcc agcagtaact actaatgcaa tgagtgtaga gccaccagct
      301 ttataaaaag cctctttcgt caaataattt gacttgattt ctcttacatc gccttcaact
      361 tgtcttaatc gagcatataa attcacttct tccgtctcct gataactaat ttgtgaatta
      421 ttatgcccgc cgtatactgc atttgcaagt aaggatagta tgattgcatc tctaggatca
      481 ttttgaatca ttggtctatt cattttgtga gcccttaatc cattctaaaa ttctctctct
      541 gtcgaaaaaa agcatattat tacagcaaac acaagtgaca ataacggctg ttctcgcttt
      601 ttggtctaac caatattttg ttccttcccg ttcattgaaa tgcaaagaat gaaattcagg
      661 agctgcaaat ttcataactt catcaggaga tggctctatt cggtatggaa tggttggaac
      721 accgataatt ttctctccgc ataattccgt aaaagtgtca tagccatcgt gtaaataatg
      781 ttcttggcta ttacaaatag ggcaagttaa attccgccca gtacgttgcg tgataaaatc
      841 cgcaaactgt tcaggtttaa tgcgtttatt attcatttta acttcctttc tacccaataa
      901 aattcaactg ttagctttgg tggactttac aaatagcttc tatgctcaac agcgacacca
      961 ataatacgaa tatcttgttt cattgagctg agtgttggaa agtcggggtt aagcggtatt
     1021 agctcaaaat gaggattgcc ggattcagac cattcgccta actcccgata acgttttaaa
     1081 gtagcctcac ctgtgccatt aaccgctgct acataatcgc ccgggtgagg acgtttgcga
     1141 atatcgatta acaccagatc gccttcacta aatttaggct ccatcgacat tccggaaatt
     1201 cttaaaaaga aagcgtccgg tccggcatca atttccgtat cgatatagtc gtagccctcc
     1261 gaatctttga aatcaaaggc ttctgtccat aaacccgctt gtatattgct gatcaacgga
     1321 tatgagttcg attttgtcgg tctggcaagc tgaacgtttg tatcaaaagc aagtgtttct
     1381 ggagaaacac caagcacttt gctgagtatt tcaatatcat caagatcggg agtacgattg
     1441 tttctctcat aattagcaat gcgagattgc ccccatttgt tatttttatc tcgattatct
     1501 attgatgtac atctttcagc aacttcatat tggctgattc ctagctgttc tctgtaagcc
     1561 ttaattctac tacctaaggt tgtcatagca ctctcctatt agattttcct cattttaaca
     1621 cgctccgtta tatccttgtg aatcacaaaa tgaaatttac aattagttca ttttgtgatt
     1681 taattaaaag atataaatca caaagagaaa tttgtatgaa taagatagct gaaatacgaa
     1741 accaaattgg tataagtcaa gctcagttag ctgttcagat tggttggggg caaccaagaa
     1801 tagctaacta tgaaactgga aatcgtaccc catctattta cgttgctcaa aaaattgttg
     1861 agggattgaa cgaattaggt gcaaacgtac gaattgatga cgttttcccc gttggcaact
     1921 aatttacccc aagaggcttg caatgccacg caatgaatta acaaaaaatg caagagcgat
     1981 tgcggatttg attcatcgaa aatccgcaac ggtgacgcat aagaaactag caagagcaat
     2041 cggactgagt gaatcacaat ttagccgtac atttgcagat aatgtggaaa tggtggcggt
     2101 gattattgat tacttaagta ttgagcttgc ggataaagac gagttactag agctgaaaga
     2161 gaaacgatat aagcgaataa atctcgaaga agcgacaaca tagaagttac caacaatgaa
     2221 aaatttttta gaaaaattca aatcattcta ccacagtaaa aattcaattt ttattagagt
     2281 ggcaattttt atgctgcttg cgtggtgggt ttttaacaat taaaaaacca ccgctggaac
     2341 ggtggtaatt tacaaggagt taccttttga aaaatcaacg caatgataca caaaaaagct
     2401 ccttatggca agagtttgaa tatcgaaaac ggttaaaaaa tctcattgaa agcggtttat
     2461 ccgtagccga aattgaaacc cgttcacaac aaatttatga ggagctgaaa agtgaatgca
     2521 caaccgaagc aaattaaccc aattttaaaa ttgcacccta aacaatcaga ggctaagaaa
     2581 gtgagtgttg atgatggtta tacacaaatt ccgaatgagt tattaaaagc aattttgcga
     2641 tcgggtgttt taggttggaa aggctcttac ttactggcta ccgtcttaaa aacattgtct
     2701 tggcataaag aaagtgattg gttcacgcat tcccaagtgt gtgaaatgat gaatattgag
     2761 ccgacaaaat accatattaa tcaactttcc gctgctcgaa aagaacttat ccgtgaaaat
     2821 atcttgtttg aagatggtaa ggaaacaggg gtaaatctca gtgtttttga atggaaaatg
     2881 gtatatcccg aaaaagtagg gagttcccga aataataggg aattaattcc cgaaaaagta
     2941 gggaatgggt atcccgaaaa agtagggaac acaaaagaaa ctattacaaa agaaaaaata
     3001 aataatgaat ttactaacgt aaattctgtg caacagcccg agacctcttt gccgaaaaaa
     3061 acaaaatctg agccggtcga ttatcaagga gtgatggaag aatttaatcg tgctgtggaa
     3121 gacacaccga ttccgcaaat tcgggcgatg agtgatgagc gaaagcgatc ggttcacgca
     3181 ttagcaaaaa tcttgcgaaa agaatttggt ggctacacca gacaacactt tgccgattac
     3241 ttcaatgatt ttgtccgaca ggcaagtagc cggaaagaca gattttattt tggcgggctt
     3301 gatggctcgg cttgggtagc aaatttcggc tacatcattc gttcgaaaac gttttataag
     3361 acttgggaag attcgctatg agccaaaatt tgaaaaatat cacttacgag gttgaatatt
     3421 tgctggttgg agcattgctg aaatccggtc ttaactcgaa agctagggac gtattgagct
     3481 ggttagagcc tgaaatgttc gctacctttc aacttggggt gatttatgag gctatccgca
     3541 agcaggcgtt gaaagataac gtgatcgata tgctgttact caataccgac tacggacaag
     3601 attttgccac gttggcagag ataatgaaaa acacgatcag cggtgcaaac ttagacggct
     3661 atgcagaaaa agtccggcaa tatcatcaac gccgagaagc ccagcgagtg tttttagacg
     3721 ttgcaggtga gttgcaaaat gcccgagacg agcagttaga tgctatcact tcaaccgggt
     3781 tagctcacct aagcaaatta ttacaacgtg gcggaaaagt gaaaccgatt gatatgaatg
     3841 atttgctcga aggttatttg gaattgttcc aagaacgtgc aaaaccaagt tttaaagagc
     3901 gtttgttgtt taccggtata gaagcattag atgggaaact aggcggtatt aacgataccg
     3961 atatttgcat tgtagcagga cgtgccggta acggtaaaac cgaaacagcc atcacgttta
     4021 ctaaaaacat tatcgagaat aaaggttcgg tgctgttctt ctcgctggag atgagcaaag
     4081 agcagattat ggataggctg attgccagtg caagcggtgt aaattcagta aaacttcgca
     4141 atccggaaat gatgaatgat gaagattttg cccgaatggg aacggcgatt cagcctttac
     4201 aaaatcagca gttgtacatt gttgataaaa gcggattaac cgcagaagag attgtggcga
     4261 ttgcagagag ccatattcaa gagtacggca aagtgagtgc ggtagtgatt gattatatcg
     4321 gtttggttcg acacggcaaa ctagacggca aaattaaccg aacctatcaa atcggtgaaa
     4381 gtatggagcg gtttaaaact ttctgcaaaa acaatcacac accgatgatt ttattggcac
     4441 agcttaaccg caatgcagat ggcagtcgcc caaccaacgc agatttgcga gacagtggca
     4501 gtcttgaaca agatgcaagc caaatcatta tggtgcataa ccaacgcaac aaagacggcg
     4561 agccagcccc ctatacagaa tggattgtta ctaaaaatcg ctttggagca aatggcacgg
     4621 tttatatgca attccagcag ggtaggttcg tagaatgcga ccaggcagaa gcgcgggagt
     4681 atttccagcc gaaagaaact aaaacatcat caaacaaacg ctatggagca atgcaatgac
     4741 agattttgat aaaaatataa aatacaaaat tatttacgca gatccgcctt ggaagtataa
     4801 ggatagtggc tgtaatggga gtgcagaaaa ccattacaca acaatgaata tcaaagaaat
     4861 atgtgattta cccgtccaag atatttcaga caaagattct attctgtttt tgtgggttac
     4921 ttaccctatg ttacctgagg gcttaaagtt gattgaggcg tgggggttca actataaaac
     4981 aatagggttt caatgggtga agacaaataa gaagaataag aattcttttt tctttggctt
     5041 gggacgatgg actagaggta ataccgaatg ctgtttaatt gcaacaaaag gaaagccatc
     5101 gagggtaagc aataaagtga gccaattaat tattgagcca atacaacatc atagtaaaaa
     5161 gcctgacatt gtcagagaaa agatagttga gttgatgggt gacttgccaa gaatagagtt
     5221 gttcgccaga aataaaaccc aaggttggga tgtttggggg gatgaggtgt gattgtttca
     5281 agctattgtt acccaaagtg cggtagggtt ttattaaaaa ggttggcggt tgtgaaggat
     5341 aacgaaggtt gggacggttt agatgattac ttataaatgc cccaaatgca acggagagct
     5401 tgaagattta agtattaatg atgaatgggg ttggttttta gatgagccgt atcgttgcaa
     5461 cggacactat acagggcggt ttccaaacat tagccgagat agcacgttaa atcgcacaaa
     5521 atcctgtggg tactttagca aggaagaggt taagaaagta aatggcacac agcgttaatg
     5581 acatcgtaaa agcccacggc aggcgttata aagcaagatt gaaaatacag atgataaaaa
     5641 tgcaaggagg cgttttagtc ccccttaatg agcgtgaagc tgaagcattg gtatcactta
     5701 aaaatggcga gcaatatgaa attgaggtta ttcgcactcg caatccagcc tttcatcgga
     5761 aggtcttcgc ctttttaaat ttttgctttg aatattggtc ggcagacaaa accgaatggg
     5821 agttttttga tgagaggaaa cagtttgata ctttccgaaa aaatctgact gttctggcag
     5881 gatttaaaga aatcacatac accctagatg ggcgtatgcg tgtagaagct caaagtctta
     5941 gttacggaaa tatggaacaa gacgagttcg agcaggttta caaagcattg attaatgcag
     6001 ctatcaagca cgttttcaac aataccaaag atgagaatag gataaatcag ttgtattcgt
     6061 ttttttaatt aaggcgaaat cgccatattt aattaggttt aattgcagga gattaaaatg
     6121 aaaaagaatg ttattagata ttggcttagc gtgccattca gaattattgc agtgcttttt
     6181 ttcttagtca ttcttactgt tagatatgta ggttatttat tgatttatcc attagcattc
     6241 tttgcagcag tcggtagttt tattgttttc tgtcaatgga catatcaaga atggctttat
     6301 tattttaagc gcggttttgc tccgatttag tgtgtgttta ttaattccct cgaattcggg
     6361 ggaaggagaa gtaagatgac aaaatttttt gaaactatcg cattcgtgat gttatttggc
     6421 attattccaa gctgtttgtt ggcgattaat actactttaa tgaatatgaa tattaaaaac
     6481 gcaccgtggt ttatggtgat tattcagtta ggttttaata tcacttgtta ttggttgtgg
     6541 tttatttctg ttatgcggaa tgttatttaa ggagattaat aagtgagcca gtgcaatgaa
     6601 ctgactgtaa ttttaatcgc catcgctatt cttagtgtat gtgccggttc actctttttt
     6661 attgagtggt ttgataagag agatggtgag ggttggaatg gctaacaaac cacctaaaca
     6721 acacaaatgc aaagagtgcg gcagttatta catcaaattt caaagcacgc agcaggtctg
     6781 ctctgttaag tgtgctatgg caatgggtaa gcgtaagaca gaggcgaaac gcaaaaaaag
     6841 tgcaaaaatc gaccgcttgg agcaacggaa gcggctggaa aaattgaaaa gccgtagaga
     6901 ttttgagaaa gatttacaaa agatatttaa taaattcatt cgcttgcgtg ataaagattt
     6961 accctgtatt tcctgtggcc gctatcatca ggggaaatat gatgcagggc attataaaac
     7021 cgttggtggt aatcctgagc tacgttttaa tgaagataat tgccacagtc aatgtgtgcc
     7081 gtgtaaccga catctgcacg gcaatattgt gaattatcga gtaaatctga ttgagaaaat
     7141 cgggcttgag cgtgtggaat ttttagagcg taaagaccac ccaccattaa aacttactat
     7201 cgaacaaatc aaagatttaa tcaaagtcta taaagcgaag tgcaaggagc ttgagcgtgt
     7261 tacctagaaa aattaaaaac aagtggttag agcctgaaaa gcagaaatgg atcgaagagt
     7321 tgctaaggtt atggggggcg tgggaatttg gcggattgga tttagaaagt cgggtaaata
     7381 tgatttaccg gctgatgaaa tctgcggagg gggttaaaag tctttcatct agagaggttt
     7441 gtaacgatag cctcggcacg ctgatcaatg agatttttac ggtagtcacc aaaagagacg
     7501 taatgttagc cgatatactc aaacaaaaat actggttcgg gcgttcggag cggcaaatcg
     7561 ctatttatta tcaactaagg gacagcgagg gtagaaaaga aagacgatgg caagaaatta
     7621 ttcaagataa atgcaaaaaa gcggaaagaa ttattgcaga tattcttgaa agcaagattt
     7681 catccagtaa ttctacagat aaactgaaaa aatatcaatt ttccccttga ttttagtgcg
     7741 taacaagtgt atattttgtg ctaatggtgg acgtagtata aatgatgttt accaatgtta
     7801 gttaaagacg cagcctgaag taatattcag ttcggaggag aataactaga aacgacgtcg
     7861 agtatagtta tttcagcgtt tgcgtggcag tatgccgacc gaattagttt aacagccctg
     7921 agcagaaatg cttggggctt ttttgtttac ggattttaag ggtgtagctc agttggtaga
     7981 gcagcggtct ccaaaatcgt tggttgttgg tttgagtcca accacccttt ccaaatgcga
     8041 gtaaatgtta tgtataacct tgatgatgca attcaagaat gcagagaaaa atttggtgaa
     8101 gataagcctt atttttctga agctggaaca cttacttttg ctggttgcaa agccctctct
     8161 cgattaagag atgaaaagtt aggaattaag gctccgccaa atttcccttt cccaagggtt
     8221 gaagatctat cttagctaca aaaatcaatg agggattgct atgacggaac aactaaatac
     8281 tcagtcaaaa agcggtgaag aagatcaaga aaaatctcaa gatcgctatg ccgtgggctg
     8341 gccatctcga cgtatatcag attgcgttca gcacttccga agtaggaaaa tttatccatt
     8401 tgccagtaat aatgacgtga agcaaggtta aatgcttctg gtggtacaga gtaaattcaa
     8461 tcatttttac cctctgctag tttatttgtt ggggaacagt attctagcag aatttttaac
     8521 caaagctcag tctcacgact gggctttttt attgcctcga aacggggtgg agtatgaagt
     8581 taatgaaaga tatgggaacg cagagctata tttggtcggg atttagcact tggctggcgt
     8641 ggctcggcga acagcagaat ttaatgttac ttagtcttgc tatcggtatt gttactgcca
     8701 ttgcagggct tattcagcga tttgatgaat ggcgtaggcg taagcgtgaa gatgagcgag
     8761 cggaagagct tcatcaagcg aaaatggcaa gattgagaaa ggggttaagt gatgagtaaa
     8821 agccttaaat atggcagtgg tatcgtttgt ggtatcgctg ccattattac attagtccaa
     8881 tatcaacatc ccgaaatccg cactaaccaa gctggattag agattatcgg taatgctgag
     8941 ggttgcagac gtgatccgta taaatgccct gccgatgtaa tcacggttgg cattggctca
     9001 acggagtttg gcggtgaaaa gattgaccca aatcgtattt attctgacaa agaaattgct
     9061 gagcgttggg caaaagattt gaagattgcg gagagttgtg tcaatcgtca ttttaatggt
     9121 aaagacatga acgataatca attttcggga atgacgtccg ctgtatttaa tatgggttgt
     9181 tataacatga gattttatcg gaataaacaa ggtcaatatg tgcagactac catccataaa
     9241 ctcgcagtga ataaacaatt tgaggagatg tgtcatcgac tacctgactt tattcgtgcc
     9301 agtggtaagg tattgaacgg attggttatc cgccgtgaaa aggagaaagc attatgctta
     9361 actggattag tggcacgata gccattgtga ttttgggctt gtgtgtatgg ttatggggtc
     9421 agtcacagat gatagatagc ttaagagctg agaaccgcgc acaagcccaa accattgagc
     9481 agcagcaaaa agccaatcaa cggctaaccg atagcttaga gcaagagcga caagcggtcg
     9541 aaaaaatcca aaaaattgca aatgagctgc ggaataaagt ggagatggcc aagaatgaaa
     9601 ttacatcaat actggcacaa gacagttgtg ctaaagctga tttgcctaat ggcgttgctg
     9661 atagcattaa gcggctgcac cagcagaaca atcacaagca ctgagtattt gtatccgccg
     9721 gcagcttacc ttgtgccgtg tgggcgaaca gaatttagcg gtagaaccta tggcgatacg
     9781 gttgagtatc tcgtcaaagt gatgggagag cgtgatttgt gtgctaatca aattgaccgt
     9841 atcagagaat ggcaagcaca aaccaaacaa gggtttaaat agctaattat ctaattaacc
     9901 gctattgaaa atgggagcgt aacagctccc tttttgtttt gatggaaagt aaatattaca
     9961 taatttagct atggtttcgg ggttggttgg ataaaacacc gaggaaatac ctaagtgatg
    10021 tgatttatat aaaacaaaac cccgaacatt tgcggtgttc gaggtttttt tattacccat
    10081 tgaaacggaa tgagtaacaa gttatggaaa ttattacatt tttaaccgta accatcaagg
    10141 agattcttat ggaatatggt ttatggcaaa taagcctagc agtaacagtc cctattttgg
    10201 cgttcgtgtc gccaaagctg attaatgcta ttgctaatct attaaatgca atgaagtaaa
    10261 ggaatttaac ccaatgacta aaaaaggcga ggttaaagcc acgtctaaag gcgtgggtaa
    10321 attaaccgat aaacagaaac gatttgtaga agagtatctg attgacttga atgcgactca
    10381 agctgcgatt agagctgggt acagcgaaaa aacagcatac tcaatcggag aagagaacct
    10441 gagaaaacct gaaatcagaa gtgcaattca ggaggctcaa aataaacgct cggagagaac
    10501 ccaaattaca caagatgatg tgctaaatgg gttgcttgaa gtgattgcaa tgagtactgg
    10561 taaaaaaatt gtgacagaaa ctgatgttgc aaaaaatgac aatggtgagt tggtcggttt
    10621 tgatattgca aaaacaaaat tcgaacctgc cgccgcaaat aaagctcttg agttactcgg
    10681 taaacattta ggaatgttta aagacaaggt tgatttaatt aattctgatg gttcactgaa
    10741 tcgaccgatg gtgattgaat taattgctcc gagtttagac gatgaaagca cagattgaac
    10801 taccacctaa actgttgcct gtttttgcca aagattatcg ttacaaaggt gcttatggtg
    10861 ggcgaggttc aggcaagact cgggcatttg ccaaaatgtc ggcagtgtct gcttataaaa
    10921 gagcaatgcg aggggaaagt ggcgttattc tttgcggtcg tgagtttatg aactcattgg
    10981 aagaaagctc tcttgaagaa gttaaacagg cgatcaagtc tgagcctttt ttagcaaatt
    11041 tttttgatgt cggcgagaag tatgttcgca ctaaagatgg gcggatttct tatgtgtttt
    11101 ccggacttcg gcataactta gatagcatca aatctaaagc ccgtattttg attgcgtgga
    11161 tagatgaagc agaaacagta agcgaaactg cttggcggaa attaattcca accgtgcgtg
    11221 agaataattc cgaaatatgg gtaacttgga acccggaaaa tagagggagc gcaacagata
    11281 ctcgcttcag gcaaaatcca cctgaaaaca gctgcataat tgaaatgagc taccaagata
    11341 atccttggtt tcctgatgtg ctcgagcaag agcggctaaa tgacaaacag cggcttgatg
    11401 atgc
//
LOCUS       DANLUA010000075        11277 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLUA010000075 DANLUA010000000
VERSION     DANLUA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            178..1974
                     /locus_tag="PXB44_001408"
     CDS             178..1974
                     /locus_tag="PXB44_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL5281884.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WVGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            2054..3115
                     /locus_tag="PXB44_001409"
     CDS             2054..3115
                     /locus_tag="PXB44_001409"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5281885.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            3103..3567
                     /locus_tag="PXB44_001410"
     CDS             3103..3567
                     /locus_tag="PXB44_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5281886.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3598..3714
                     /locus_tag="PXB44_001411"
     CDS             3598..3714
                     /locus_tag="PXB44_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL5281887.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3730..4197
                     /locus_tag="PXB44_001412"
     CDS             3730..4197
                     /locus_tag="PXB44_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5281888.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4252..4932
                     /locus_tag="PXB44_001413"
     CDS             4252..4932
                     /locus_tag="PXB44_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL5281889.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(5000..6142)
                     /locus_tag="PXB44_001414"
     CDS             complement(5000..6142)
                     /locus_tag="PXB44_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL5281890.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(6152..7531)
                     /locus_tag="PXB44_001415"
     CDS             complement(6152..7531)
                     /locus_tag="PXB44_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5281891.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(7653..8747)
                     /locus_tag="PXB44_001416"
     CDS             complement(7653..8747)
                     /locus_tag="PXB44_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5281892.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(8851..9303)
                     /locus_tag="PXB44_001417"
     CDS             complement(8851..9303)
                     /locus_tag="PXB44_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL5281893.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            complement(9324..10076)
                     /locus_tag="PXB44_001418"
     CDS             complement(9324..10076)
                     /locus_tag="PXB44_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL5281894.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(10087..11271)
                     /locus_tag="PXB44_001419"
     CDS             complement(10087..11271)
                     /locus_tag="PXB44_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL5281895.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
BASE COUNT         3248 a         2595 c         2356 g         3078 t
ORIGIN      
        1 aatcacgaat aaatatattt ttcttcctcc atgacgagat tataaaaata aaatgtgatc
       61 tcgctcacaa ataattaaaa ataatctttt ttaatctttt tctagcgttt atcattaatt
      121 ccgacaactt ctctggagtt ttttagttct attaatctct caacaaagag gccccctatg
      181 gactttttaa ccgaaattct ccttttctta aaggatcaag tgctgaataa agcacccttc
      241 ttactaggta tcgtagcttg cttaggttac attcttctta aaaaagacac ttctacgatt
      301 attaaaggca caattaaaac aattgtcggt tttatgattg tacaagccgg ttcaggtttc
      361 ttagtaacca actttaagcc ggttattgat ggcttagcca aatatcacca acttaccggt
      421 gcagtaattg acccttacac cagtatgcaa tcgaccattc aaaccatggg cgagaactac
      481 gcttgggttg gttatgccgt aattggggcg ctgttcctca atattctatt agtggtgttc
      541 cgccgtttaa ccggtatccg taccattatg ctcaccggtc acattatgtt ccaacaagca
      601 ggcttggttg cggtgttcta tatgatcatc ggagcgtcaa tgtgggagac cattatctac
      661 acctcagtgt tgatggctct ttattggggt atttcgtcta atattatgta taaaccaacc
      721 caagcggtaa caggcggtgc aggcttctca attggtcacc aacaacaaat tgcttcttgg
      781 attgcagtca aactcgcacc aaaacttggc gataaagacg atactgtaga taaaatgaaa
      841 ttgcctaact ggctcaatat tttccacgac agtatttctg caaccgcatt agtaatgacc
      901 atcttctttg gaatcatctt actttcattc ggtttagata acctcaaaga acttgctggc
      961 aaaaccaacc agtttatgta cattcttgaa atgggcttga aatttgcggt ggcaattcaa
     1021 atcattgtaa ccggcgtacg tatgtttgta gctgagcttt cagaagcctt caaaggtatt
     1081 gctgaacgct taattccaaa ctcagtatta gcgattgact gtgccgctat ctacgctttc
     1141 tcgccaaatg cgatggtatt cggctttatg tggggggcta tcggtcaatt tgtggcagta
     1201 ggcggcttgc ttgcagtagg ctcaccggtg cttattattc cgggctttat cccgatgttc
     1261 ttctctaacg caaccattgg tgtgtttgca aaccaatttg gtggttggaa agcagtaatg
     1321 aaaatctgtt ttgtgatggg tattattgaa gtgcttggct cagcttgggt tattcaaatg
     1381 cttgcttcac aaggcacaac ctttaacggt tggatgggta tggctgactg ggcattgttc
     1441 ttcccaccaa tcctacaagg tattatcagc attccgggct tcttctttgt aattgtggca
     1501 gcagcaatgg tttatatgtt cttcgcatct aaacaacttc gtgccgaaga agcagaagcg
     1561 gcagctcgtg gcattacact tgaacaatta gacggctacg gcttagatga agtaaaagca
     1621 gaaccaaaag cggaggcagt agtacaacaa gcggtcgaaa atgccgaaaa ttctgcaaac
     1681 ccaatccgca ttctagcggt gtgtggctct ggtcaaggct catcaatgat gatgaaaatg
     1741 aagatcaaag gctatctcga caaacgtggt attccgaata ttatggattc ctgtgcggta
     1801 actgattaca aaggcaaatt aagcgaggtt gatattattg tctccagcaa acatttagcc
     1861 aacgaaatcg aagtaccgga aggcaaagcg gtattaggtg tacaaaatat gctaaatcca
     1921 aactcatttg gtgatgaatt agttgagcta atcaatcagc ataatgcaaa ataagtaagt
     1981 ttaggggtga ataatcaccc ctatctatgc taaagattgt aaaaactcac ttcatcaaac
     2041 gaggaattat ccaatgaaaa aaatcttatc caccctgttt atgctctccg cggttagcgt
     2101 tgccgttgcc aaagaggtaa acattaaact acttggtacc tcagacgttc acggacgtgt
     2161 tgtgccgtgg agctacgggg cagacgtgga agacaaatcc ggctcattcg cccaaattgc
     2221 aacctatgtc aaagaggtgc gtgccaacca caaaaatgtg ttgctgtttg atattggcga
     2281 tgcgattcaa gacaaccaag tcgaagagtt cgctaaaacc ttagacaaag cgaaaaacaa
     2341 tcccgtgccg aaagtgctga atgcaatgaa ttacgacatc ttcattctcg gcaaccacga
     2401 atttaacttc ggaatgccga atttagatgc gattttaaaa gacatcaacg ccaaaattct
     2461 cactgccaac ttctatcaca aagacggcaa acgctacgtt gagccaacta ccatcattga
     2521 acgtgatggc gtaaaacttg ggattatcgg cttaaccacc ccaatgtcgg ctatttttga
     2581 aaaagacacc ggtcatttag acgcaatgaa gttcacttcg ccgattgaag aggcgaaagc
     2641 ccaaattgcc gatctcaaag ccaaaggcgt ggacgccatt attgtgctgg cacatatggg
     2701 gattgagaac gaaaacaaca ttcccgacac cggcgtggcg gatttagtga atgcagtgga
     2761 cggtattgat gtaattatcg ccggccatat gcataaagat gtgccgagtg aaactatcaa
     2821 aaataccctg attaccgaac cgcaccgcta cggcacagtg gtttccgaag ttgatcttgc
     2881 ctttgatgtg aatgacaaag gcgaagtgaa actactttct aaaaccgcca aaaccgtacc
     2941 ggtgaagaaa ctcgcaagcg atccggaaat cgaaaaaatt taccaaccgt atcacaatga
     3001 actgcgtgcc ttaaacaacg tcaaaatcgg tgaaactgca caagatttaa tcccacaagg
     3061 caaaatcacg gtgtatcgat tgctttctcg caagacaccg gaatgtcctc attgatccac
     3121 gatgtgcaac aacattacag cggtgcagac gtagtgagtt ttgcatttga ctacgaaacc
     3181 gtgcgtttag acaaaggcga catcaagaaa aaagacatca tctacaacta ccgctatgcc
     3241 ggtggcgatg tgagcatgta tgagctaacc ggcaaacagc ttaaacagta tatggaatgg
     3301 tcagcggatt acttcgacac cattcaagcc ggtgacaaag actaccgcta caacgctgtg
     3361 cggggtaaat ctaaatacgt taccttcgac ctattcggcg gcgtaagcta caacatcgac
     3421 ctacgcaatc caagcggtag caaaattgtc gatctaaaac tggcaaacgg cagtctaatc
     3481 accgatgacg gcaaatacaa agtgggaatg aactcctacc gctttggtca gctcaccaaa
     3541 aaaggcggca tctgggaagg acagtaaatc cccgtgattt gggaatccaa agtcgccatg
     3601 ggggctgaaa aaggcacaat ccaaaatatg ctgattgatt acatcaccaa cgtcaaaggc
     3661 ggaaaaatcg aaggcaaatc gcataaccgc tgggcgatta tcggcttaaa ttaacgaaag
     3721 ggaaacacta tgaacttaaa acaatctctt attgaaaata actctatttt gctcaatcaa
     3781 tcagcggcga attgggaaga cgcaatcaaa atcggcactg atttattagt caaagccggt
     3841 acgattgaac cacgctatta cgataatatt gtaagcaaaa tcaaagagat ggggccttat
     3901 attattcttg caccgggctt ggcaatgcct cacgcccgtc cggaagaagg tgtgattaaa
     3961 acctctttcg ctttagtgac ttttgacacc ccgatttatt ttgagggcga agatgagccg
     4021 gttcacgtta tgctaaccct tgcagggagt gactctgatc agcatatgca aggcttggta
     4081 gaaatcaccc aagtgctaga tgacccagag agcgacacag gtgttgattt agataaaatc
     4141 cgccgctgta aaacggctga agaagtgtat gcagtaatcg atcaggcatt agcctaatat
     4201 tctacaagcg gttcaaaaac atcaatattt tgcaaataga ggaatacaat tatgtccaaa
     4261 ccattattac aaattgccct tgattctctt tcactggaaa aagcggtggc ggatgcaaaa
     4321 caagcagaaa accaagtcga aattattgaa tgcggtacta ttcttgcttt tgctgagggt
     4381 atgaaagccg tttccacctt gcgtgtattg cacccaaatc acattattgt gtgcgactta
     4441 aaaaccaccg atggcggtgc aattttagct aaaatggcat ttgaagccgg tgccgactgg
     4501 ctcacggttt cggctgcggc tcaccctgca accaaagcgg cttgcaaaaa agtggcggac
     4561 gaatttaacg ctgctcaccc ggagctaaaa gtgaaaaaag agattcaaat tgaaatctac
     4621 ggcaactgga cggtggaaaa agatgccaaa gagtgggtag aattaggggt aacccaagcg
     4681 atttaccacc gctcccgtga tgcggaacta gcaggtaaag gttggacaac ggaagatgtt
     4741 gagttaatga aacagctttc tgacttaggc attgaattat ctatcaccgg cggtatcgta
     4801 ccggaagata ttcacctgtt caaagagatc aaaaacgcca aagcctttat tgcaggccgt
     4861 gctttagtcg gtgaaaaagg caaacaaact gccgaagcca ttcgtgtgga gattgataaa
     4921 tactggaact aatccggcat tgaaacccaa taaaaatggc gttctctcaa ccggaacgcc
     4981 attttttatc tcattttttc tattcttgca aggccgatat tttccacaat ctcgctacat
     5041 tttcggcagt tgaaatcagg ttctctttgc ctgattttaa caccgcttct aaattgctga
     5101 cctgtcgaat aatcggaaat accgcatcaa tgccgtgttg gtacaccaca gggtaatctt
     5161 ctttcaatga gccaactatc gcaatcaccg gcttgccaaa ctgcttagcg actttcgcca
     5221 ccccaatcgg ggttttaccg gcgatagatt gagcgtccat tcgcccttcc cctgtcacca
     5281 ccaaatccgc atcggcaagt ttctcagcaa gatcggttgc ctcaagaata atttgcacgc
     5341 cggaacgtaa tgttacgtta ggcagtagca gtaaaccacc gcccattcca ccggctgcac
     5401 cggcaccggc tttctcggca atctcaatgc ctaattggga ttttacggtt tcggcaaaat
     5461 gcaacagggc gtggtcgagc attttaacca tttccggtgt agctcctttc tgtggcccaa
     5521 aaattgccga agcccctcgc tcgccacaca gcggattatc gacatcgcaa gcaacctcaa
     5581 attccacttg ctttaagcgt gaatccaaac cgcttaaatc aatctgctga atttggctta
     5641 atgcctcgcc accaaagccg atttcccgct gttcggcatt tagaaatttc gctcctaagg
     5701 cttgtagcat tccgacaccg ccatcgttag tcgcactgcc gccaatgccg agaatgattt
     5761 tttccacccc taaatctaag gcggctttaa ttaactgccc cgtgccgtag ctggtggtaa
     5821 ccagcggatt tcgttgctcc attggcacta aatgcaagcc acttgccgcc gccatttcaa
     5881 taaaggcggt tttttgatcc cccgacaagc caaaaaagcc ctccacctca tttgcaagcg
     5941 gtgcaattac gctgattttt tgcaaactgc cgtgagtggc atcaaccagc gactgtaccg
     6001 agccttcgcc accgtctgcc atcggtacga gctgatactc cgcctcaggg aaaacacggg
     6061 caaaaccctg ttgaattgct tgagccactt ctaatgcggt caaactctct ttaaatgaat
     6121 ccggtgcaat cacaattttc atcgtcaatc cttagagtaa aatcaatgaa agcagataaa
     6181 ccgtgagcat acccaccaca cccataatga aagttagggt agtttgcgta cgatagcctt
     6241 gttgcggagt cagtttagta aagtttgtta ccacccagta gtagctgtca ttggcgtggg
     6301 aaacgcacat tgagccagcc gcaatcgcca ttacggttaa ggctgccgcc atttcactgg
     6361 ttagccctaa ggcgttcatt aatgagtccg gtgcgttgaa cattcccata atggaggcgg
     6421 tggtgataat tgccaccgtt gagctgcctt gtgcggtttt taaaattgcc gaaatcagga
     6481 acgggaagaa aatccctgct gtactgataa tatgagcgtt ttgcttgatg taatccacaa
     6541 agccggcttg agtaatcacg ttacctagca caccaccggc ggcggtaata aagagaattg
     6601 ggccgactaa tttcagggta tcattggtta agctgtcaaa ttccgccatt ttaccgcttg
     6661 aaaccagtag gaaaatagcg aagatcaccc caattgctaa cgcaataatc gggttgccga
     6721 caaattgtag gaatacgccg aaatcgccac ttacaccggc aattttggca atcgagccga
     6781 tagccattaa gataatcggc ataaaaatcg gggcaaagct taaaaaccca cttggtaatt
     6841 tgccgtattg tttgatcaga tcttcatagc tttggctgat tacttgatcc gcttgagctt
     6901 cttccatcac gctgacttgt ttgccaacat atttggcaaa gaagtaactg gcaatcaaca
     6961 caggaatcga aaccacaaag cccattccga tcacaagcag gagattatgg cttaaaccaa
     7021 cagcaccggc tgctgcaatc gggcccggtg tcggaggaat aaagacgtgg gaggcatata
     7081 aaccggcact taacgccacc gccattccca ccggattggc aagaattttt ttgcgaatag
     7141 cttcacgaat cgggtttaac accacaaagc cgctatcaca gaacactggg ataccaacca
     7201 cccagcccat aatcaacatt gccagttcag ggcgtttttg ccccaccact tttaccacca
     7261 tatcggctaa tttgagggct gcaccggttt tttctaaaat cgtgccgata atcgccccaa
     7321 aaataatcac gataccgata cttttgaagg tattactaaa accttcgccc acaatagttg
     7381 gaattttggc aagcggtaaa cctgccacta atgcaagggc tatcgaaatc gacattaagg
     7441 ctaagaacgg gtgaactttc cacttcgcaa tcatataaat cattatgatg atagcgacca
     7501 gaaagatcag aataagtgca atacctgaca taataagtct cctcattgta gaaaaatgaa
     7561 cttattattc ggtatttatt gctaaaaaga tttagataaa tttgcaaaaa atttgttaaa
     7621 aacgaccgct tgtttgtata tttttacaat ttttacgaca gaaccgctcc aagatacagc
     7681 acaaattttt gctctatgtt attgaaagat aaaccagtta tctgctcaat cttctctaaa
     7741 cggtaacgta gcgtatttgg gtgaataaac aattttttgg cagcgtgatc cagatcacaa
     7801 tttgcgaaaa aatagtaacg cagacttttc agtaacacct gttttttatc ggaaaccagt
     7861 aaagcttgga acggcgagag caaggttttc gcttcccaag agtggctgaa actgtgcaag
     7921 agggccggca gtttaaaatc ttcaaaatga ataatctgct tacggctttg gatctgctcg
     7981 gcataattga gggtatggca ggcggtttgg taggaaatat gggtttgcga taaattttcg
     8041 accgccagcc caaccaccgt tttgaaggaa atatccggat cgttaagttt gtttagtgca
     8101 ttgcggatta acgatttttc gttggataaa tgattcagca aggcaatttt atctaaccca
     8161 atcacggcgg tgagtaggct cgggaaataa tgttcgaaaa aatcaatcag gcgttggagt
     8221 ttttccgcag tagagccgtg aattttaatc accatcaccg acagcggttt tgcaaaatct
     8281 tgcccgaaaa agaccgcttg ttgctggatt tctgctgtgc ttaggctacc tttgagcaag
     8341 gcccgcacaa actcttcccg atagcggcgt tgccaacgtt cctgttccaa ctcaaacgct
     8401 tgctgcataa tcagctccgc cgccattttc accaattgag catattgccg aacttgttgt
     8461 ggctgccctg aaatccccac tacaccaatg gttgagccta aatagtgaag cggtaagtta
     8521 atgcccgctt tcacttcatc gcaccactgt tttgcaagat tttcatcaat ttcgaccgct
     8581 tgatcgttgc gtaacgcaat caccgctcct gtgtgtcgtt gcccgatacg accgacatcg
     8641 ccggaagcaa taatcacccc ttcactatcc atcacattga cggagttggg aataatctgc
     8701 atcgtgcgct tgacgatgcg gtcggcaatc gcttggttga gtttcattat atgattccta
     8761 ctcattccca atactatgaa aaatccctct ttcgagggat ttgcaaatta tctacgcttc
     8821 cacttcctga gaatagatca tagcgtgaag ttattttgcc acagtcgctt taattaattt
     8881 ttccgctttg gcaaggttgt tttctacatc agcataatcg gtattagcgt gagccaccac
     8941 ttgatcggca cgatttaaca ttacttcgac cttgcccttg ttcggttcgg taattaacac
     9001 ttttaacggt aattgcaacg ctaacatcgg atctttcacc attaatggcg taccggcttt
     9061 cggtgtgccg taaaccaata cggttgccgg ttgcatttct aagccggctt gttttgccgc
     9121 cgcttggtga tcgatttcgg caaaaatcgt cattcctttt tcttttaagg tgttggttaa
     9181 agttgtgact gtagtttgga aatcaaattt actttcaaat accaccggtt tgacatcggc
     9241 aatatctgcc attgccacgc tgctcattgc taatgcagaa actgcgactg cttttaagtt
     9301 catttctttt ctcctcaaat agattaaata atcccttgtt cttccaactt gcctaagaaa
     9361 taaggcatct gtttacgcca agtcggccag tcatggtcaa tatcaaagcc ccagtagtca
     9421 aaccaagcat cgatcccttt tgcgttaaaa gcctcttgta atcggcgggt gtcttcaata
     9481 tgctgttctt cccacgcacc ttgtccgact gcaatcacat agtgattttg gcggtagcgg
     9541 tctaagaacc aatcgtcatt ttgaccccat acataatcaa tcggcgagtt aaaataaacc
     9601 gctttatcgc cataaaactc accactgaaa aagcgagcgt cataaacacc gcttaaggcg
     9661 atggcaatat caaacagatc cggatgacgt aaggcaaagt ttaccgtatg gaaagccccc
     9721 atactacaac cggtggcaat catcgtgccg ttccagtgcg attcatggcg gattaacggc
     9781 actagctcgt gaataatata acggtcgtaa gcattatgag ctaatgccat atcgtgtccg
     9841 gatttatgtt tagataacca agattccgca tcgtaagaat caggcgtgta aaatttgatt
     9901 aagccacgtt caataaagga acggcaagcc tcaatcattc caaaattacc atactcgttt
     9961 tcattgccgc cggaggaagg gaaaacaata accggtttac ctgcgtgtcc gtaaacgttg
    10021 aacgacattt cacgacctaa ttctccactc cagtggcttc ttttttcaaa atgcatactt
    10081 cacctcttac tgtttagctt gtgcaaattg tgcaatttca cgcatttgct ccattgtttc
    10141 ggtacgggct aaaatgcctt gttcgcccat cactttggca aatacgcccg gtacgctttg
    10201 cacgctgata atattgccgg caaatttctc acaaacagct tcaatcgaat tcacataatt
    10261 ttggtttgcc ttgcgggaaa tataaaccac gttccaaggg tgctgaatat cggcatagaa
    10321 tttgttttct ttcacaatat tggcaaattc acggaacaca tcgaaatcgt tggcgtagtt
    10381 ccacatatca atagttaagc cacccggcgg acggcagttg atttcgagtg gtaacaactc
    10441 ttcttttttg ctccctttaa cacggaagaa ctcaaaatgg aaaaaacgct cttttacgtt
    10501 aaaggctttc acacattgtt tgcccatttc cactaatttt ggcgaaattt cacatgggac
    10561 atagtagaac atatcgccat cttttgaaac ggtatctaat accgcttctg aatattcaag
    10621 gctggagtag aaaacgatat tgccctcttt atcggttaaa ccgtcaaagg taataatatc
    10681 gccgtcaata aactcttcca taatatattc aaccgttggc gttttataac cgaagaaagc
    10741 ttctaactct tccgcagttt tgattttgta agtatcgctt gccccaaccc ctgaatttgg
    10801 cttgataatc accgggaatt taagctgttt tgctaatttg cgggcatctt catcatcttt
    10861 aaatactcgc ccttgtgcca ctttcaagcc ggttttgcgg aatacctctt tcatttgggc
    10921 tttggtttta attgagagca tatcatcgct cttataacca aacacattga aatcggtacg
    10981 caatttcgca tctaactcca gccaatattc attgtgcgat tcaatgcggt caattcgccc
    11041 gtatttatgg gcaaagtacg cgaccgcacg ataaacttgt tcgtaatctt ccatattatc
    11101 tacacgataa tattcagtca gggaatgacg cagattttcg ctcaatgatt cataaggggc
    11161 atctgcaatt cccaatgtat taaaaccggc ttcacgcaaa cgaatggcaa aggtttcaaa
    11221 gttcgttggg aagtgcgggg aaatcatcac aaaattaagt ggtctagaca tagttct
//
LOCUS       DANLUA010000076        11229 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLUA010000076 DANLUA010000000
VERSION     DANLUA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            155..373
                     /locus_tag="PXB44_001420"
     CDS             155..373
                     /locus_tag="PXB44_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281896.1"
                     /translation="MYQYYLAQVGNEQQKLIRGMPDNLLSFSVYEPEQEDWDNKFGPF
                     WADKILISGFDAYEEISEKEAIRFIKVH"
     gene            374..787
                     /locus_tag="PXB44_001421"
     CDS             374..787
                     /locus_tag="PXB44_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL5281897.1"
                     /translation="MCQAEQFARQLHYGQTDKAGKPYIEHLAFVAENISTKNEETEAV
                     AWLHDSVEDTQCSINDIRHIFGDVIADAVEAITKKQGESYFCYLDRVKSNPIAKVVKL
                     ADLTHNLCLERLPKITEKDLKRAEKYKSALEYLQA"
     gene            905..1639
                     /locus_tag="PXB44_001422"
     CDS             905..1639
                     /locus_tag="PXB44_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281898.1"
                     /translation="MKLKLDENGHVVVENGMPVYIHDDGKEIPFDALKATQKITQLNA
                     EAKQHREAKEKAEADLKAFSGIDDPKAALEALKTVANLDAKKLIDAGDAEKVKAEIIK
                     GYDEKLAVATAQAEKLQQQLHAELIGGSFARSKYAQEHLNIPSDVVQAFFGKHFSISD
                     DGKVVAKFADGGEIFSRTRPGEKADFEEALEALVSAYPNKDAILKPSGSSGAGAGGGS
                     RSSSMPKTLSDCKTDEERIAYLQANS"
     gene            1705..2652
                     /locus_tag="PXB44_001423"
     CDS             1705..2652
                     /locus_tag="PXB44_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281899.1"
                     /translation="MAFDLQVFNKQTQLALTETVDQAIEKFNAASGGAIILQNKPAEG
                     DFDIRASFKGIAGLVRRRNVYGTGTVDAKRLEQLLDVAVKVAAGTAPIEYQPSQYKWI
                     LLNPELAAIEIGTQLAKARVADMLNTAIAGTVAAISGNTAMTLDEKTAAPTFRILNKG
                     AAKMGDRAGSLKAWVLHSTTLHGLYDNALTNAENLFKYENVNVIRDPFGRVFVVTDSP
                     ALMASDGTFNTLGLVENAVVVSGNNDFDSVLVPKTGGENLSAVYQAEWTYNLGILGYK
                     WDMTAGGKSPNDTALGTPTNWDKSVTSNKDTAGVLVKTK"
     gene            2712..3062
                     /locus_tag="PXB44_001424"
     CDS             2712..3062
                     /locus_tag="PXB44_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281900.1"
                     /translation="MKRILYFTTDFSAKNIAFAKAHGVLMRNLNAYHPSDTLENADAV
                     CGNVPQAYQHLPLFELPESNQKSGQDLKTLKTEEIKEKLTELGIAFEANAKKETLLTL
                     LTQAVGRDGTNDAK"
     gene            3052..3489
                     /locus_tag="PXB44_001425"
     CDS             3052..3489
                     /locus_tag="PXB44_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281901.1"
                     /translation="MLNSYMTVEQANAYHDIRMSREAWNALSDQEKQQRLVSASDFLD
                     FNYRFSGEKADLTQEREFPRKGFEAIGIPKAVQFAVCELALQDNLTANQEAQMSSVRV
                     GPVSVNYDNSSPQNSVNRFEYVRQLLSTWLDRSSFGTVKLERS"
     gene            3489..3860
                     /locus_tag="PXB44_001426"
     CDS             3489..3860
                     /locus_tag="PXB44_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281902.1"
                     /translation="MADFYGNLGKISHSLIKQFGQPCSVTINEQGRYNPATGERKKGS
                     STVHAGYCLFDNLAFDFRNQQSVSVQQGDVMIYLTADCKPVLNAVIEANGEKWSVINV
                     QPIRPAATTMIYQVQGRKADG"
     gene            3853..4266
                     /locus_tag="PXB44_001427"
     CDS             3853..4266
                     /locus_tag="PXB44_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5281903.1"
                     /translation="MGSFHAQIARFVEQTQQKADRALRVIALQTLSGVQKKSPVDTGQ
                     LRRSWTVALNGFPSSYNGSQTVLNSAKFGDMIVIATNKPYAPMLEYGLYPNPPKKPTG
                     KTKNGYSIQAPNGMVRITVQEMQAFIRNNPQLGVS"
     gene            4266..4658
                     /locus_tag="PXB44_001428"
     CDS             4266..4658
                     /locus_tag="PXB44_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281904.1"
                     /translation="MRPIIRSVLEQHLAELNPFNTVWEGIIDSPKLPYQTVSMTTSTN
                     ETATIGNTPKAKQSGFLQVTLYYPLDQGTKAIEERADEIFRHFFGQVWIEENVQILVQ
                     QPPLVASLYRLDNSIALPITIYFTAYEL"
     gene            4668..5690
                     /locus_tag="PXB44_001429"
     CDS             4668..5690
                     /locus_tag="PXB44_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281905.1"
                     /translation="MSILGNSQGTKRTVVFAKESSFGTKAAPTGAKIMPRVETSLNTN
                     FESYQSEEIRDDLQRSASVIGFEKVEGDIKGELAAGQWAAFFAAALRGSFTAQAKKPI
                     ITKTTDGTGEKNGKILIVPKTSHTSDSFTLEDVFKDINVSRQYLGCRVSKLSLDVQPN
                     GIASITVSFLGQRGEESTTAYFTNPTDLTQSGKLAGVKGSLQVNKRPVGLVTAFKLDI
                     DLGASSEPVLGAKYAPDVFIGTVAVSGSFTMYFQDKTMIDAVRNGTDLSLALRMDAEE
                     RTNTDYLTIILPGVKVTSNELDDGAKNIMQTLNFDAFPAVWDSASQIDNVLKVATTMI
                     VQDSLA"
     gene            5780..6181
                     /locus_tag="PXB44_001430"
     CDS             5780..6181
                     /locus_tag="PXB44_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281906.1"
                     /translation="MNLNALSKEKQLAEHRFELVHPVSGEPLGVFMSVVSAKSDKAQR
                     FAEKQLRQEQMRELENAKSRKPKFKGLGDMIAESRAMAIDRLTGWENVEWNEKPLEFS
                     DQNAELLLTECDWIVEQIFEHSNDLGKFLKS"
     gene            6196..6522
                     /locus_tag="PXB44_001431"
     CDS             6196..6522
                     /locus_tag="PXB44_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281907.1"
                     /translation="MYAEKTFKLDKKLKGSESTLREHLQAIEMQTGITPEELHNPEPS
                     PAVAYLLGWFFELAMSRQSGMSLNPISYTEIDAWSRLFARHLEQWEISVIKQLDMIYL
                     NVQQTE"
     gene            6524..6847
                     /locus_tag="PXB44_001432"
     CDS             6524..6847
                     /locus_tag="PXB44_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5281908.1"
                     /translation="MDTFNFQPNWGLNVQRKPEVMKIAFGDGYEQVAPKGLNHNLRTY
                     SAVFTGTEARIKQIEAFFERQGGYKAFLWTPYGGKEGKFRCEEWEVSISSGKWTLSAT
                     IREVL"
     gene            6934..7626
                     /locus_tag="PXB44_001433"
     CDS             6934..7626
                     /locus_tag="PXB44_001433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281909.1"
                     /translation="MKKIFVSALLLTCSLSALGNSSYELIIDSMQDLEKHKPEILSIP
                     EADRNTFIRYMMRKELRIKLNQGRLADGLTVREAIQNQQAFEKDRNSKMQQEKQKKET
                     EKAEKEKAVTDFNQKFELSITGARKAKNALNQDILLVSLSLKNNSDKPIIAVQPDIQF
                     VLGEDKQTISSMRPKKFENGIPPNGSEIFEFGFDLDDMFALKAEKNIEKLRAVFQTAE
                     VLFADGTTEKLQ"
     gene            7690..8514
                     /locus_tag="PXB44_001434"
                     /pseudo
     CDS             7690..8514
                     /locus_tag="PXB44_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            10558..10716
                     /locus_tag="PXB44_001435"
                     /pseudo
     CDS             10558..10716
                     /locus_tag="PXB44_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            10724..>11229
                     /locus_tag="PXB44_001436"
     CDS             10724..>11229
                     /locus_tag="PXB44_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5281910.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRNLKDKSGNAGRVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLITSIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNA"
BASE COUNT         3535 a         2201 c         2639 g         2854 t
ORIGIN      
        1 agttgaaaaa cggtaaattg cacgagctct atgaaaaatt cagagatctg aatatacctc
       61 ttgcaaaaaa atcagaaatt ttacttgaaa tgcagaagtt aagtgctaat ctaaaagagc
      121 caaagaacat tgatttaatc aaggagaaaa aataatgtat cagtattatt tagctcaagt
      181 tggcaatgaa cagcaaaaac ttattcgtgg tatgcctgac aatctactta gtttctcggt
      241 ttatgagcct gaacaagaag attgggataa taagttcggc ccattttggg cagacaagat
      301 cttgataagc ggatttgacg cttacgagga aatttcagaa aaggaagcaa tccgatttat
      361 caaggtacat taaatgtgtc aagctgaaca attcgctcgc caactgcatt atggacaaac
      421 agataaagct ggaaagccat acattgagca tcttgctttt gttgcagaaa acattagcac
      481 aaaaaatgaa gagacagaag cagtagcttg gttgcacgac agtgtagaag atactcagtg
      541 ttcaattaat gatattcgtc atatatttgg cgatgttatt gctgatgcag ttgaggctat
      601 tactaaaaag caaggcgaaa gttatttttg ctacttggat agagtaaaat caaatcctat
      661 tgctaaggtg gttaagttag ctgatttgac tcataatctt tgccttgagc gattgcccaa
      721 gatcactgaa aaggatctta aacgagcaga aaaatacaaa tcagcattgg aatatctaca
      781 agcctagcct aaaacgctag gcttttttat cgcaattttt taccccaaac tgaccgtttg
      841 tgagccatca caggcggttt tttattatcg ggcggaagcc caccagcaat gagaggaagc
      901 tcaaatgaaa ttaaaattag acgaaaacgg acatgtagta gttgaaaatg gtatgcctgt
      961 gtatattcac gatgacggaa aggaaattcc gttcgatgca ctaaaggcga cacagaaaat
     1021 cacacaactg aacgctgagg caaagcaaca tcgagaagct aaagagaaag cggaagccga
     1081 cttaaaagca ttctcaggga ttgacgatcc gaaagctgca ctcgaggcat taaagacggt
     1141 tgccaattta gatgctaaaa agctgattga tgccggtgat gcggaaaaag tcaaagcgga
     1201 aatcatcaaa ggttacgatg agaaattagc tgtggcaacc gctcaagccg aaaaactgca
     1261 acagcagtta cacgctgaat taatcggtgg ttcgtttgct cggtctaaat acgctcaaga
     1321 acacttgaat attccgtctg atgtcgtaca ggcattcttc ggtaagcatt ttagtatttc
     1381 ggatgacggc aaagtggtag ctaaatttgc tgacgggggc gaaatcttta gtcgcacccg
     1441 cccgggcgaa aaagcagatt ttgaagaggc attagaagct ttagtaagtg cttatccaaa
     1501 taaagacgct attttaaaac caagtggttc aagtggggca ggagcaggtg gcggatcacg
     1561 cagttcatct atgccgaaaa cattatcgga ctgcaaaaca gatgaagagc gtatcgctta
     1621 tcttcaagca aattcgtaag gtgcaagagg ttgcaccttt tttattttcg gtgcaatcgc
     1681 accacttttt agaggaaaat caaaatggca tttgacctac aggtttttaa caaacaaacg
     1741 caattagcct tgaccgaaac tgtcgatcag gctattgaaa aattcaatgc agcatcaggc
     1801 ggtgcaatca ttttacagaa caagccagct gaaggtgatt ttgatatccg tgctagcttt
     1861 aaaggtattg ccggtttagt tcgccgccgt aatgtgtacg gtacaggtac ggtagatgca
     1921 aaacgtttag agcaattact ggacgtagca gttaaagtgg cggcaggtac agcaccgatt
     1981 gaataccagc cgagtcaata taaatggatt ttattaaatc ctgaattggc ggcgattgaa
     2041 atcggcacac aacttgccaa agcccgtgtt gctgatatgc tcaatacggc tattgccggc
     2101 acggttgctg caattagtgg caatacagca atgacacttg atgaaaagac cgccgctccg
     2161 acattccgca ttttaaacaa aggtgcagcg aaaatgggtg atcgtgccgg ttcgcttaaa
     2221 gcgtgggtgt tgcactccac aaccttacac ggcttatacg ataatgcctt aaccaatgcg
     2281 gaaaacttat tcaagtacga aaacgtgaac gtaattcgtg acccgtttgg gcgtgtattt
     2341 gtcgtcacag acagcccggc tttaatggct tcagacggta catttaacac cttaggcttg
     2401 gttgaaaatg cggtggtagt ttccggcaac aatgactttg acagcgtgtt agtgccgaaa
     2461 accggcggag aaaacctttc agcagtttac caagctgaat ggacctataa cttaggaatc
     2521 ctaggttata aatgggatat gaccgctggt ggtaaatcac cgaacgatac cgccctaggc
     2581 acaccgacga actgggataa atcggttacc tccaacaaag acaccgccgg tgtattggta
     2641 aaaacgaagt aatttttaac cgcttgtaga taaggtttac aagcggttgt ttttttatct
     2701 ggagtttgca aatgaaacga atactttatt ttacaacgga tttttcggca aaaaacattg
     2761 cctttgccaa agctcacggt gtattaatgc ggaatctgaa cgcttaccac ccatcagaca
     2821 ccttagaaaa tgcagatgca gtgtgcggta acgtacctca agcttatcaa catttaccgt
     2881 tgtttgaatt gcctgagtct aaccaaaaat caggtcaaga tttgaaaacc ttaaaaaccg
     2941 aagaaattaa ggaaaaactg actgaattag gtattgcttt tgaggcgaat gccaaaaaag
     3001 aaacattact aaccttactc actcaggcgg tgggacgtga cggaacaaac gatgctaaat
     3061 agttatatga cggttgagca agccaacgcc tatcacgaca tcagaatgag ccgtgaggcg
     3121 tggaatgcgt taagcgatca agaaaaacag caacgattgg tgtcagcgtc tgattttctg
     3181 gatttcaatt atcgtttttc aggcgaaaaa gcagatctta ctcaagagcg tgaatttcca
     3241 cgcaagggtt ttgaagctat cggtattcct aaagccgtgc aatttgcggt atgtgagttg
     3301 gcattgcaag ataacttgac cgcaaatcaa gaagctcaaa tgagtagcgt aagagttggc
     3361 ccggtttcag ttaattatga taacagttca ccgcaaaaca gcgtgaatcg gtttgagtat
     3421 gtgaggcagt tgctttccac ttggttagat cgatcttctt tcggcacggt taagttggag
     3481 cgtagttgat ggcggatttt tacggcaatt tagggaaaat ctcccattct ttgattaagc
     3541 aattcgggca gccttgtagt gtaacaatta acgaacaagg cagatacaac cctgcaaccg
     3601 gtgagcggaa aaaaggtagc tcaacggttc acgcaggcta ttgtctgttt gataatttgg
     3661 cgtttgattt ccgcaatcag caatctgtca gcgttcaaca aggcgatgtg atgatttatc
     3721 tcactgccga ttgcaaaccg gtattaaatg ctgtgattga agcaaacggc gaaaaatggt
     3781 cggtcatcaa cgtccagcct atacgcccag cagctaccac gatgatttat caagtacaag
     3841 ggagaaaagc cgatgggtag ttttcacgct caaatcgccc gttttgtgga acaaacgcaa
     3901 caaaaagcgg atagggcgtt aagggtaatt gccctgcaaa ccttaagtgg ggtgcagaag
     3961 aaatcgcctg tcgataccgg acaacttcgg cgtagctgga cggtggcatt aaatggtttc
     4021 ccgagcagtt ataacggcag tcaaaccgtg ctgaatagtg ctaaattcgg cgatatgatt
     4081 gtgattgcta ccaataaacc ttatgcacca atgctcgaat atgggcttta cccgaaccca
     4141 ccaaaaaaac cgaccggcaa gaccaaaaac ggttattcaa tacaagcacc taatggaatg
     4201 gtgcgaatta ccgtgcagga aatgcaagcc tttatccgca ataatccaca attaggagta
     4261 agctgatgcg accgattatc cgctcggtat tagagcaaca tcttgccgaa ctcaatccgt
     4321 ttaatacggt ttgggagggc atcatcgaca gcccgaaact gccgtatcaa accgtatcaa
     4381 tgacgacttc aaccaacgaa accgccacga ttggtaatac gccgaaagcc aaacagagcg
     4441 gttttttgca ggtcacgctt tactatccgc tagatcaggg gacaaaggcg attgaggaac
     4501 gagccgatga gatcttccgc cactttttcg ggcaggtctg gattgaggaa aatgtgcaaa
     4561 ttctggtgca acagccaccg cttgtagcct cactttatcg actggataac agcattgcct
     4621 tacccatcac catctatttt accgcttatg aactatagga gaacccaatg tctattctag
     4681 gcaattcaca aggcacaaaa cgtacggtcg tttttgccaa agagagttct ttcggcacga
     4741 aagctgctcc gacaggcgca aaaattatgc ctcgtgttga aacctcactc aataccaatt
     4801 ttgaaagtta tcaatcggaa gagatcagag atgatttaca acgctccgcc tctgtgatcg
     4861 gctttgaaaa ggtcgaaggc gatattaaag gcgaactagc cgccggacaa tgggcggcat
     4921 tttttgcggc tgcattacgt ggttcattta ccgctcaggc gaaaaagccg attattacca
     4981 aaaccacaga cggcaccggc gagaaaaacg gtaaaatttt gattgtgccg aaaaccagcc
     5041 acacctccga cagtttcacg ctggaagatg tcttcaaaga catcaacgtc agccgccagt
     5101 atttgggatg tcgtgtatcg aaactcagcc ttgatgtgca accaaacggt attgcctcga
     5161 ttacagtctc atttttaggg caacgaggcg aagaaagtac aacagcctat tttaccaatc
     5221 ccaccgattt aacccaatcc ggcaagctgg caggggtaaa aggctcattg caggtcaata
     5281 aacgcccagt cgggttagtg acggccttta aactggacat tgatttaggg gcaagcagtg
     5341 agccggtatt aggggcaaaa tacgcccctg atgtctttat cggtacagtg gcagtgagcg
     5401 gatcgtttac gatgtacttc caagacaaga cgatgattga tgcggtgcgt aacggcacag
     5461 atttatccct tgctttgcgg atggatgccg aagagcgtac caataccgac tatttaacca
     5521 ttatcttacc gggtgtcaaa gtgacctcaa acgagttgga cgatggggcg aaaaacatta
     5581 tgcagacact caattttgac gcattccctg cggtatggga ttcagcaagc caaatcgaca
     5641 acgtgctaaa agtcgccacc acaatgattg tgcaagacag cctcgcttaa tcgaaatttt
     5701 gcaaaaaaat aaccgtatct aaccgcttgc cctcaaacaa gcggtctttt ttatccccaa
     5761 tttacaaaag gaaacccaaa tgaatttaaa cgcattaagt aaagaaaaac agttagccga
     5821 acaccgtttt gagctggttc accctgttag tggcgaaccg ctcggcgtgt ttatgagcgt
     5881 cgtgagtgct aagagtgata aggctcaacg ctttgccgaa aaacaattac gccaagaaca
     5941 gatgagagag ctggaaaatg ccaaatcccg caagccgaaa ttcaaaggct taggcgatat
     6001 gattgccgaa agtcgtgcaa tggcgattga ccgcttaacc ggctgggaga atgtcgaatg
     6061 gaacgaaaaa ccgttggagt tttccgacca gaatgcggag ctacttttaa ccgaatgtga
     6121 ttggattgtg gagcaaatct ttgagcattc gaacgatctg ggaaagttct tgaagagcta
     6181 attgatgatt tacaggtgta tgcagagaaa acgtttaagt tggataaaaa acttaaaggc
     6241 agcgaaagta ccttgcgaga gcatttgcag gcaatcgaaa tgcaaactgg cattacacca
     6301 gaagaattgc ataaccctga acccagtcca gcggtggcct atctgctggg ctggttcttc
     6361 gagttggcaa tgagccgaca atcgggtatg agcttaaacc caattagcta cactgaaatt
     6421 gatgcgtgga gcaggctttt tgctcgtcat ctcgaacaat gggagatcag cgtaattaag
     6481 cagcttgata tgatttactt gaacgtacaa caaacggagt agtatggata ctttcaactt
     6541 tcagcccaat tgggggctta acgtacagag aaagccagaa gtgatgaaaa tcgcctttgg
     6601 tgacggttac gaacaagtcg cccctaaagg gttaaaccat aatctccgca cttattccgc
     6661 tgttttcacc ggcacagaag cacggattaa acagattgag gcattttttg aacgacaagg
     6721 cggctataag gcttttcttt ggacacctta cggcgggaaa gaggggaaat tccgctgtga
     6781 agagtgggag gtaagcataa gcagcgggaa atggacgctg tcggcgacga taagagaagt
     6841 gttgtagttt tttctgattt tgtgaactgg ttcacactct gtgggttgga tataggttag
     6901 aatttaagtc taatttgctt aacggagtct tttatgaaaa aaatttttgt ttcagcctta
     6961 ttgctcactt gctcgttatc tgcacttgga aattcctcgt atgagttgat tattgactca
     7021 atgcaagatc ttgaaaagca taaacccgaa attctatcta taccagaagc ggatagaaat
     7081 acttttattc gttatatgat gcgaaaagaa ctgcggataa agttaaatca aggcagatta
     7141 gcggatggtc taactgtgag agaagctatt caaaatcaac aggcatttga aaaagataga
     7201 aatagcaaaa tgcagcagga gaagcaaaaa aaagaaacag agaaagcaga aaaagaaaaa
     7261 gctgttacgg attttaacca aaaatttgag ctatccatta caggggcgag aaaggcaaaa
     7321 aatgcactaa atcaagatat tttgcttgtt tcattaagtc taaaaaataa ctcagataag
     7381 ccaattatcg ctgtacaacc ggatattcaa tttgtactgg gtgaagataa acaaactatt
     7441 tcatccatgc gtccaaaaaa atttgaaaac ggcattccgc ctaatggctc agagattttt
     7501 gagtttgggt ttgatttaga tgatatgttt gctttgaaag cagaaaaaaa tatagagaaa
     7561 ttgagggcag tcttccaaac agcagaagtt ctttttgctg atggaacaac tgaaaaactt
     7621 caatagtaat tggtaaatct tcattggccg cttgtagaaa tacaggcggt ttttttattg
     7681 gagcaagcta tggcagattt agcaacactg gcgattaaaa tcgaaacaga aggcgcaagc
     7741 cgagcaagtg cagatctaag taaggtcgag caatctgcaa agaacacaga gaaagccgca
     7801 gataatctga cgcgcgcaat gggaaatttg aagcggattt tagcgattgc agggctggct
     7861 ggtgggttat ccgcttacct agatatggct gaaagaatgc agtcgttaaa cgcacagctt
     7921 aaatttgtta ctaactctca atacgaattc aatagcgcac aaaaagagct ttttgcgatt
     7981 gcgcagaata cgcgcgctag tttggaaagc accacgcaac tttatattaa atcttctcag
     8041 gcactgaaag attacggcta cgcgcaaaag gatattctca catttaccga aaccattaac
     8101 aaagcaatgg cagttggcgg ggttaagcca caagagcaag ctagtgcttt actccaatta
     8161 tcacaggcat taggttcagg gaaactgcaa ggagatgaat tccgttcaat ttcagaagct
     8221 gcaccgatta ttttagatac gcttgccgag tatatgggta aaagtcgcga tgaagtaaga
     8281 aaactcgcat ccgagggtaa gctcaccgca gacttactgt tcaatgcgtt taacggttca
     8341 agcgctaaaa ttaccgaaaa atttaatcaa atgccgctaa catttggcgg tgcaatgcag
     8401 cagttgcaga atgccacatt gaaatttatc ggtgacctag atagtgcaaa tggcttgagt
     8461 ggaatgttag ctcaagccat tagtttttta gcacagaatt ttgatgtgtt aggcaaggct
     8521 ttgatttatg caaccggtgc ttatattgcg tttaatgcag ttgcctttgc tcagaatttt
     8581 gcaaaagcaa ccggtggtgt cggtttatta agtagtgcat ttggttattt gactacgatg
     8641 atccgaggtg caactgttgc aatgatggct aatcctatcg agcttttaac aactgcaatt
     8701 attggggctg cttttgtatt tgatgctttt atcagtgata tagaggtagg tgcagcaacc
     8761 tttgatgcga cttggggaga tgttgcgaca gctgtgtggg atgattttaa agatttaact
     8821 tcagatgcgg cagattggtt agtcactcaa tgggataaag caaccgccag tgcagcgagt
     8881 tcgttcagtt ccttaggtgt tgatattggc tcagtatggg agacggtgca atccattacg
     8941 aaaacattga tcaatagtat tattggtctg tttgttggag cttatcaagc catcgtgctt
     9001 gcttggaata attttcctgc cacaatggaa aacttgggtg tgatggcggt aaatgctctt
     9061 gtcgatttgg ttcaaaaagg catcaatagc attattcagc ttatcaaatt accgattgag
     9121 cttttaaaca gagcctcatc tacttttggt ggaggaaatc tgattgatac atcaggttgg
     9181 aaggcaagct tagatgattt taaactaaag gcaagccgcg aggcagaagt tgttggtcaa
     9241 tcaattagcc aagcatttgc ggatagtatc accacagatt acataggcaa tgcagttaat
     9301 tcagtgagtg actatattga tggagcggca gttcgtgggc gcttaaaacg tcaattagat
     9361 gaaaccgcct ctgacgatca aggtacgcag tcaccagcta aaaaaacatt agctggaaat
     9421 aataactctg ctagtgactg gaaaaactac ctcaatgaac ttgaacgagc caatgccgat
     9481 ggattagccc gtattgcact tgagcaagat cgctcaatgc gtgaaatgtt ggaaaaggcg
     9541 aagaaagccg gtgcaagtca cgctgaaatt gaaaaagcca aaatgctgat tacagaacgt
     9601 tatgctaaag agcgaatgga aattgccgag aagtatgtgc cgagtctgaa gtttgaggaa
     9661 gaactcaaag atcagattaa ggaaattgat gaactgcaaa agttaaacct gattaccacc
     9721 gagcaagcag gtcttgcgaa agaggcgtta gcaggcaaat atcagccggt agttgaaatc
     9781 caacagcaat acatcaatca actgagagag attgaagcat taaaacaagc cggtgtatta
     9841 agcaaacctc aagcagaagt agcggccgaa aaagccaaat gggacgccgg cacacaaaaa
     9901 gccaaaatcg caggtaaaaa cgccgttagc ccttatgaaa aatggaaatc ggaatttgac
     9961 ccaatgcagg caattcaaaa tgagcaagcc tcaaagttgg cagaagtgca atcgatgtac
    10021 gatcagcatt tgatccaata tgaggatttt atcaacgcta aagcacaaat tgatgctaag
    10081 gcgactgctg atactcatca actgtttatg aacagtatta gtgggtttgg ttcggcgata
    10141 gatacgatgt taggcgtgat gaaaaacgca gggcaagagc aatcaggtat ttaccgcacg
    10201 atgtttgcga tgtcgaaagc gtttgctatt gcggaatcaa ttctgaactt gaatcgtgca
    10261 attactcaag caatgagcga tcaaacagct attacaccgg ctcaaaaatt tgcaaatatg
    10321 gcggctattg ctagtgccgg ggcgagcttg atttctaata tccagtcagt ctcattaggc
    10381 ggtatggctc acagcggtat tgacaatgtg ccgaaagaag gcacttggtt gctggataaa
    10441 ggtgagcgtg tagtggatag tcgtactaac caagacttaa aaaccttttt atccaatgct
    10501 aatcgtggcg gtgcaagtgg tcaaagatcg gcgataaatg tcaaaattat taataacggg
    10561 caacccgtag aggcgaaagt cagcagtgaa gagactgtag atggcaatat ccaaatgacg
    10621 gtggaactgc tgaagaaaat ggatcaaatc gccgatcaac gctaccgtag aaatcagtta
    10681 aatgatttaa gaacaggtgg aacattatct aaataggtga agtatgccaa ttagtatttc
    10741 taaccaaatg aaactagatc ttgccaaact tgagcaaaat gcaatgctgg acttgtacga
    10801 ggttgatttg cgcaatctca aagataaaag tggtaatgct ggcagagttt accgctttta
    10861 ttctgggcta aatgaactca aaacaagtat cgtctggcaa gggcggactt acgatccgta
    10921 tcctattgag gcaagtggat ttgaacgaaa cagtagcggc ccgagcaatc gcccgactct
    10981 cacgctatca aatctgtttg gtttaattac cagtattgcg aatcagtttg acgaatgtat
    11041 cggtgcgatt gtccgccggc atcaggttta tgctcaatat ttagatgccg tgaattttgc
    11101 cgggggtaat gcgaaagctg atccaaatca ggaaataatc agtcattttg tgattgagca
    11161 gcttaccagc ctcacccgag aaacggcgac ctttacgctg gcattaccga ttgaaaccga
    11221 taatgccaa
//
LOCUS       DANLUA010000077        11187 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLUA010000077 DANLUA010000000
VERSION     DANLUA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..552
                     /locus_tag="PXB44_001437"
     CDS             <1..552
                     /locus_tag="PXB44_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5281911.1"
                     /translation="PEEIDIKSKNGKVAKQFCQRKNLPLYITEISKERIRRAGKQILQ
                     NNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDPVSEKADTQAVLYELL
                     LRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVIAQQPKKVIALDRLFN
                     GNDARKKNTELQMQDAGITFFVI"
     gene            562..3198
                     /locus_tag="PXB44_001438"
     CDS             562..3198
                     /locus_tag="PXB44_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5281912.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            3330..5762
                     /gene="gyrB"
                     /locus_tag="PXB44_001439"
     CDS             3330..5762
                     /gene="gyrB"
                     /locus_tag="PXB44_001439"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL5281913.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(5815..6777)
                     /gene="cmoB"
                     /locus_tag="PXB44_001440"
     CDS             complement(5815..6777)
                     /gene="cmoB"
                     /locus_tag="PXB44_001440"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL5281914.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(6874..8637)
                     /locus_tag="PXB44_001441"
     CDS             complement(6874..8637)
                     /locus_tag="PXB44_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL5281915.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
     gene            8901..>11187
                     /locus_tag="PXB44_001442"
     CDS             8901..>11187
                     /locus_tag="PXB44_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL5281916.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
BASE COUNT         3499 a         2191 c         2408 g         3089 t
ORIGIN      
        1 cctgaagaaa ttgatatcaa gagtaagaat ggaaaagtag caaagcaatt ttgccaaaga
       61 aaaaatttac cactatatat aactgaaatt tcaaaagaac gtattcgccg tgccggaaag
      121 cagattttgc aaaataaccc cgaaaaccga ccgcttgatg taggttttaa agtcttcaaa
      181 ctgacagaaa gccattttaa acaatggcaa tcgcccacat ctgaaaattt agccgatcaa
      241 cttgaaatgt ttattgatcc tgttagtgaa aaagcggata cgcaagccgt tttatatgag
      301 cttttattgc gtttagggtt aaaactcacg gcaaaagtgc ggttggaaaa taaggtgttt
      361 tgggtggaag aaaacgggct gattttcgcc ttgctgttaa atgcagccga cgaagaaatt
      421 attcaaactg ttatcgctca acaaccgaaa aaagtcatcg ctttagaccg tttattcaac
      481 ggcaatgatg cccggaagaa aaacaccgaa ttgcaaatgc aagatgccgg cattacgttt
      541 tttgtgattt aaggagaaaa aatggaactc caatttgaaa ccttagagta tcaattacaa
      601 gccgttaatg ccacagtaaa tttgtttgtc ggacagccga atgccgcaac tgaattttca
      661 ctcaaagccc aaaatgatat gcggtttgtg ccgaatttag gcttgcagat tagtgatgag
      721 cagttacaac agaatttggc taatctgcaa aacaggcaaa aaatcgaccg cactttgctt
      781 gtcgaacagg gaaaaaattt taccgtggaa atggaaactg gtacagggaa aacctatgtc
      841 tatttacgca cgatttttga attaaaccgt caatacggct ggcaaaaatt tgtgattgtc
      901 gtgccgagtg tggcaattcg tgagggagtg ttgcatacgt tggaaactac aaaaagccat
      961 tttaatacgg tatttgataa cccgagcgtc aatcaaaaat ttgaatataa aagtaaccaa
     1021 acttcacgct taaaatcctt tgctagtgct aatcatattg aaattttagt gatgaatatt
     1081 gatgctttca ccaaagaaag taacgtgatt aataccgtga atgaaagcgg tgatgctccg
     1141 attttctata ttcaacaggc gaatccgatt gtgattattg atgaaccgca aaatatggaa
     1201 acggagattc gccgtaatgc gattgagagt ttaagtccgc ttttcacttt gcgttattct
     1261 gccacccata aaaatgcgta taacccgatt tttagcctca atccggtgca agcctatgaa
     1321 atgggattag ttaaacaaat tgaggtagat agcgtattgg cggaaaatga ggtaaatgga
     1381 gcttatcttt ccctcaaaga gattaaaaca ggggcaaaaa gctggtcggc aaaaatcgaa
     1441 attttattta acgataaaaa agcgatgaaa aagaaagtga ttagcgtgaa accgaatcag
     1501 gatttgtatg agctttccaa ccaaaatgaa atgtatcgtt cagggtttat attaagcgga
     1561 atggataccg atagccaaat ggttagcttc tccaacggca cacaaatttt tcaaggggcg
     1621 gaacattccc aacttaaaga agatgtgcaa aaaatgcaga ttcgccgcac tattggcgag
     1681 catttgcaaa aagaaaagaa attacgaccg cttgggatca aggtgttgtc tttgttcttt
     1741 atcgataaag tggataatta tcgcccttct gcacaaagcc ctacgggggg aaaatttgca
     1801 aaatggtttg aggaaattta tttggagcta gccaaagaag agcctcaagg cgtgcataac
     1861 ggttatttct cacaggataa aaaaggcgta gcaaaagata ctaatggcac aacgcaagcg
     1921 gataacgaga cttatcaact gattatgcgg gataaagaaa aattgctttc gctggagaat
     1981 ccgttacggt ttattttctc gcattctgcc ttaaaagaag gatgggataa tccgaatgtg
     2041 tttcaaattt gtactttaaa cgatactgca agcgaaatga aaaaacgcca agagattggg
     2101 cggggcttgc gtttgcctgt gaatcaacaa ggattgcgga tttatgatcg tcatctaaat
     2161 acgctgacaa ttatcgcaaa tgaaagttac gaacaatttg ccgccacatt acagcgtgaa
     2221 attgaggaag attgtggtgt acagtttgcc aaaagcaatg ttaaaaaacg ggaagataaa
     2281 aaagccgttc gctaccgtaa agatttcagc ttacatccgg aatttgcggc aatttgggaa
     2341 cggattaaac accaaaccac ttatcgggta gatttcagta atgcggaatt gattgaaagt
     2401 gcggtggaaa aaatccgcaa aatgcccgat atcgaggcga caaaaattaa gcattccaaa
     2461 gtgaaattgg aaatgaatga aacagggatt tcaactaatt atcgctcatc taatgaaaca
     2521 gcggtaaata cacaatgggc gatacctgat ttattgagtg agattcaaaa gaaaaccggg
     2581 ctgacgcgtg cgaccctttg caaaatttta caaaaatccg accgcttgca agaagtcttc
     2641 cgcaatcctc aacgctttat tgatttaatt gcagagaaaa tcaaccttgc tttacaacaa
     2701 attatgacag acggggtgga atatcacaaa attgccggaa aaacctatga aatgacgtta
     2761 ttcggcgatt ttgattttta cctcaatcaa tatacttttg aagttcaaga taaaacaaaa
     2821 acgatttatg aagactatat tgcactggat tcccaaacag aaaatatctt cgcccgacat
     2881 tgtgaatcta gtgagcaagt ggagttttat tttaaattac ccaaacgttt taaaattcca
     2941 acgccattag gcagctataa tcccgattgg gcagttgtgt ttaagggaga aaataaaatt
     3001 tacttggttg ccgaaacgaa aaatacaggc aaaggtattc aagcaggagt agatgaagaa
     3061 aaattagatg aaagtgaacg aatgaaaatc gactatgccc gtaagcattt caaaggctta
     3121 cagagagatt ataaggattt ggattatagg gttgtacaga aagtggagga gctgtctgcg
     3181 gagtattgta aaatttaatc tgtggtcttg tagaattgag atagactgca atttcaacta
     3241 aaatttaacc gcttgttttg cgatactttt acgcattttt ctgctataat cggcacaatt
     3301 ttttcgattt caaattagta ggaataagaa tgtctgaaca aactcaagca acaagttatg
     3361 attcttccag tattaaggtg ttacgcggct tagatgcggt gcgtaaacgc ccgggaatgt
     3421 atatcggcga taccgatgac ggtacgggct tacaccatat ggtgtttgag gtggttgata
     3481 atgcgattga cgaagcatta gccgggcatt gtaaagacat tattgtcaca attcattccg
     3541 ataattcggt ttctgtgcaa gatgacggtc gtggaattcc ggtggggatt cacccggaag
     3601 aaggcgtttc ggcagcagaa gtcattatga ctgttcttca cgcaggcggt aaatttgacg
     3661 ataactccta taaggtttcc ggcggtttgc acggtgtggg cgtatcggtg gtgaatgcac
     3721 tttcctctaa attacaactc actattcgcc gtgaaggaca cgttcacgag caattctata
     3781 gcttaggtga gcctgatgca cctttagcga ttatcggcaa taccgaaaaa acggggacgt
     3841 cggttcgttt ctggccaagt ttggatattt tcaaaaataa aaccgaattt gaatataaaa
     3901 ttctgtctaa acgcttgcgt gaactatcat ttttgaattc aggcgtttcg attaaattaa
     3961 ttgatgaacg agacggcaaa gaagagcatt ttaaatatga gggcggtatt aaagcctatg
     4021 ttgagtattt aaacgaaggc aaaactcata ttcacaatac gccattctat ttgaccactg
     4081 agaaagacgg cattggcgtg gaaatttccc tacagtggaa tgacagttac aacgaaaatg
     4141 tgtactgctt taccaataat attccacagc gtgatggcgg tacgcactta gccggtttcc
     4201 gtggggcatt aactcgtgcg ttgaaaaact atatggataa cagcggggta ttgaaaaaag
     4261 ccgatgcgaa cattgatgcc tcaggtgatg atgcccgtga aggtttagta gcggtgattt
     4321 cggtgaaagt gccggatccg aaattctcct cacaaaccaa agataaattg gtgtcgtcag
     4381 aagtgcgtgg agcagtggaa agctcaatga atgaggcttt atccgactat ttagccgaaa
     4441 atccggacga tgcaaaaaat atcgttacca aaattattga tgcagcccgt gcaagagaag
     4501 cagctcgtaa agcccgtgaa atgacccgcc gtaaaggggc gttagattta ggcggcttac
     4561 cgggtaaatt agctgattgt caggaaaaag atccggcgtt atcggagctt tacttggtgg
     4621 agggtgactc tgccggtggc tcggcaaaac aagggcgtga ccgtaaaaac caagcgattt
     4681 tgccgttaaa agggaaaatt ctgaacgtgg aaaaagcccg ctttgataaa atgctttctt
     4741 cacaagaagt agcaaccttg attaccgctt taggcacagg catcgggcgt gatgattata
     4801 atttggaaaa attacgttac cacaaaatca ttattatgac cgatgcggac gtggatggtg
     4861 cacatattcg tacgctttta ttaactttct tctaccgtca aatgccggag ctgattgaaa
     4921 acggctatat ttatattgca cagccaccgc tttataaagt gaaaaaaggc aagcaagagc
     4981 gttatattca agataacgat gaaatgtcgc aatatgaaat tgatattgca ttagaaggtg
     5041 caggcttata tgtgagtgaa gatgctccag cgttaagtgg cgtagcgtta gagaacttaa
     5101 ttgcccaata taacggcgta caaaaattaa ttgaacgttt aagccgtcgt tacccaacat
     5161 tattacttaa cgaattaatt tattcaaaac cgctttcggt ggaatttgca aaaaatcaag
     5221 caaatatgac cgcttggagc gagcaatttg tcgctacatt gatggcaaaa gaggtgggcg
     5281 gcagtttcta ccgtattaat accttgttca atgaagaacg ccaaatttat gagccggaaa
     5341 ttattgtgac tacacacggt atggacagta cctatcgttt agattacaac tttatcaacg
     5401 gtaatgaata cgctcgcatt gtggcattag gcaacgagct gaacgattta ctatctgatt
     5461 cagcctatgt ggtgcgtggt gaacgtcgcc aagaaatcag cagttttgct gaagccttag
     5521 attggttggt gaaagaatct cgtaaaggct taactattca acgttataaa gggttaggtg
     5581 agatgaatcc ggaacaatta tgggaaacca cgatggatcc ggtggctcgt aaaatgctac
     5641 aggtcaatat taccgatgcg attgcggcag ataaactctt cagtacgctg atgggcgatg
     5701 aagttgaacc acgccgagat ttcattgaaa ctaacgcttt gtatgctcag ttagatattt
     5761 agttaaaaaa tccgcatttg acatgcggat tttttattct cttaaactag cattttaagc
     5821 attcgctaaa atcaccgccc gcttcggagc agggtagcct tcaatggttt tgctatgatc
     5881 gtttggatct aaaaaatcca ctaaactttc attttccaac caatcggttt ttcgctgctc
     5941 ttctagtgta gtgattgcct catcaacaca acgcacattt ttaaagccga ctttttccag
     6001 ccaattaatg agtgcagcaa cagaaggaat aaaatagata tttttcattt tcgcatagcg
     6061 atcagccggc actaaaacat cattaatatc gccatcaatc accagcgttt ctaacaccaa
     6121 ttcaccgcct ctgactaatt gggctttgag ttgagaaaga tgatcaagcg gtgatttgcg
     6181 atgataaagc acccccattg aaaacacggt atcaaaagca gcaagcggtt gcatttgctc
     6241 aataccaagt ggaatcagat tagcccgtct atcattccct aagagtttcc gcactgcctc
     6301 aaattggcac aggaacagct cggtcgggtc aataccaact accatttttg ccccttcgcc
     6361 taccattcgc cacatatgat aaccgctacc gcagccgaca tctaaaatgg tgcggtcttt
     6421 taacggtgca agatgcggaa gcacacgatc ccatttaaaa tcggaacgcc attcggtatc
     6481 aatatgaatc ccgtgtagat gataagggcc tttacgccac ggcattagtt gctttaaatg
     6541 gtgggtaatt tgcttgactt ccccctcaga taaaggcttg cttgaaatag actcaacctt
     6601 gtcttttaga ttgatacaag cggtcgaatt tggtaaaaaa tctgcaactt ttgcccactt
     6661 ggcatagtca gcgtgggtgg tcttttccca ttgtttgagt tgtaagggta aggtttctaa
     6721 ccaagcagaa aggtcggttg tggcaatttg ttgataaaaa ggacgaaagt caatcatttt
     6781 gaaatttatg tctaggggag agacctatgc cctcccgaaa ggtagtaaat agaaaggaca
     6841 ggcacagaac ctacccctac gaaaaatata aaattatctc gcaatcggat ttactttcgc
     6901 tttttgcact ttgcgataag cgttcagtaa tttctgatgt gcagtcatat tttcaagcga
     6961 ttcgtccgat gcagttaaac cgtggaacgg gttaccgcct tggattgcac cccaagcagc
     7021 ttcaaccgct tgtttaccgt acattttctc aaacaccata cgatattgtt ccgaatcacg
     7081 ctcttcatct aagaacaact caatcgattg gattaagcag cggtaataat tgttacgttc
     7141 tgctgagaat acgcttgcat tcatattcgc tgtccagttt gcccagtctg aggcttgctc
     7201 taagttacct aaagctaaat gcagcatcga tttaagctca ccgatacgca atgtttgcat
     7261 tgcagagcct ttctctgcca ccacgccaat aaattcacgc acacgggtta aatcatcaat
     7321 gctttgctca tctaattcat caagcagttc ttggtaagtt tcagcatcgt gatggaaatg
     7381 tggcaaatct aacaagattt cacgccaatc catacccata ttgttattgg catagaccaa
     7441 atcatcgctt ggatagatat tcgacatacc cggtgcaaga atacggcaag cgtacacacc
     7501 taaatgctca taatccataa tatacacagg ctgattatgc ttgttgaaaa tcgccatcat
     7561 attattaaat tcttgctccg ttgtgccgga gaagtcccaa tgcacgaacg gataatccgc
     7621 atcacgtttg aaaagatccc acgaaattag accgcttgaa tcaataaagt gtgtctcaag
     7681 gttcgcatga tctgctacat cttcattatt aaatgaaggt ggggcaaaca catctaaatc
     7741 ctttaagcta cggccttgta ataactcggt tacggtacgt tcaaacgcca cttggaaatt
     7801 cgggtgtgca ccaaaagagg caaaacaagt accgttattt gggtttaaca agatcacaca
     7861 aataactgga aattctccgc ctaatgaagc gtcatagcat aaaatcggga agtcttcttg
     7921 ctctagtttc tcgatagagg ctttgattgt cggataaccg tcaatcacac tttgcggaat
     7981 ttccggcaag ctgattgcct cagtaataat acggtttttg acaaaacgct cgaacacttc
     8041 ggataagccc tgcactcgag cttcattttt tgtgttgcct gccgacatac cgtttgaaac
     8101 aaacaggttg ccgataatgc tttgcggaat gtaaaccgtt tcattgtcag attggcgaac
     8161 gtaaggtagg gtaacaattc cacgatcata atttcctgat tgtaaatcaa ccaataattc
     8221 cggcgtgatt tcaccattcg gatcaaaata ttcccataaa aactcgtcca tcatctccat
     8281 tggtggaatg gcttcatcat caatcggaaa ccatttttcg gttggataat gcacaaattc
     8341 actattagca atctcttgac ctaaataaaa atccgcccag aaatagttgg tagataaacg
     8401 ctcaaaatac tcacctaatg cagaggcaag agcggctttt ttgcttgctc ccttaccgtt
     8461 agaaaaacat tgcggacagt ctgcatcacg aatatgcacc gaccacacat tcggaacagg
     8521 atttaaccaa gaggcttctt caatattaaa acctaaggtt tttaatttat gctgaaaggt
     8581 cgcaatgctg tcttcaagtg cagcatcttt accggtaata aaagtttgtt ctgacatgat
     8641 aataccttta acttgtcgta aaaatggcaa cattctagca aaaatcccca tagataagta
     8701 aattttttgt gaaatttctg ctgaaagaac agatttcaaa acttagaaaa aatgattaca
     8761 agcggtcaaa ttttacttga attttgcaag tgaaatttgt gtttgattta gattaagaaa
     8821 atattatcta ggatatatac agaataaaag gttagaggta gtattgcttg tgttaggaag
     8881 gaaatattga aggtataaaa atggaagaac atactattca gtctttttat gcccattcgg
     8941 ggaagctttc agatcattca gattggcaaa ctttactcag ccacgccaat aatgttggct
     9001 tgcttgccgc cgaatttgct gcgccgtttg cttcacaaga aattgctcgc tgcacggggc
     9061 tattgcacga tttaggcaaa tatagtgaac gttaccaaaa gcgtttacat ggcggagaac
     9121 gggtaaatca ctctactgct ggtgcaaaaa ttgcagtgga acgttggggg cctttgggca
     9181 aaatgatggc gttttgtatc gcaggccacc acgctgggtt agcaaatggt tctggagaag
     9241 ggaaaaatcg ttctacactt acacaacgtt tgcaagagca atttggctat ggtcataaag
     9301 atctaccggt acttgacgct atttggcagc aagaacttaa tttgcctgaa aaattgccct
     9361 taccgccgtt aaagccttct aaaaacgaac ctttcttttc ttacgccttt tttatccgaa
     9421 tgctctattc ttgccttgtc gatgccgact ttcttgatac agaagctttt tatgccaatc
     9481 tcgaaaaaag accgcttgaa cgaggaaatt accccaattt aaccgcactc catcagcaat
     9541 ttaatcaatt tattgcacag tttagggagc gaattgccac tcaaacgcct aatacgcagg
     9601 aagcacaacg taaagcggaa gtaaaccgct tgcggagtga aattttggat tatgccgtat
     9661 ctcaagcaaa acaggaaaca ggtctgtttt ctctgactgt gccaacgggc ggtggtaaaa
     9721 cctttacttc aatggcgttt gcccttgagc atgctaaaca gtatggttta tgccgcatta
     9781 tttatgttat tccgtttacc agcattattg aacaaaatgc tgcggaattt cgtaaagctt
     9841 ttggcgaatt aggaacagag gcggtattgg aacatcacag cacttttgat cacgatagcc
     9901 tacctgataa atcaagccga gataagctga aattggcatc agaaaattgg gatactcctg
     9961 ttgtggttac aactgcagtg caattctttg aaagtttgtt tgccgaccgt tcttcacgct
    10021 gtcgcaaatt gcataatatc gtcggtagcg tgattatttt ggacgaagcc caaatgctac
    10081 cgttgaattt attacgccca attatggctg cgattgatga attagctcgc aattatcaat
    10141 gttcggtagt gctttgcaca gcgacacaac ccgctattca tcaagcgaac ggtttttata
    10201 aaggatttga aaatgtgcga gaaattgccc ctaatccaac cgcacttttt gaaaaattga
    10261 aacgtactac ggtaaagcat atcggtattc aaacagatgt ggagttgcaa agcaaactcg
    10321 cagataaccc acaaatgctg ataatcgtga ataaccgccg tcacgcccga gcgttatatg
    10381 attcagcgaa acagttagag ggagcttatc acctcaccac gctaatgtgt gctaaacacc
    10441 gctcacaaac gctgactgaa attcgtcttc gcctaaaaca aaacctacct tgccgagtga
    10501 ttgccacttc tttaattgaa gccggtgttg atgtcgattt cccactggtt atgcgagctg
    10561 aagcaggact agattcagtg gctcaagcag cagggcgttg caaccgagag gggaaacgtt
    10621 tatctgtaga aagcaatgtt tgggttttcc aacctgaaga acagtggaaa gcaccaccag
    10681 agctaggctt actttctggc tgtatgcgtt caacggttca ggttcacgca gatgatctac
    10741 ttaatccgaa agcaatcaat cattatttcc aagcggttta tgaggctaaa ggaaaagagc
    10801 tggataagcc aaatatttta aaggcgtgtc acgacgctgg gcaaagcctt aatttcccat
    10861 tccaaaaaat agctgaagat ttccgaatga tcacaagcca tttaattccg gtgattatcc
    10921 ctttcgacca agaagccgaa aaccttattg accgtttacg ctatgcggat caagtaggcg
    10981 gtattttaag aaaactccag ccttacactg tgcaagtacc ggaaaaagca ttagccgaac
    11041 tctataacgc tggcagagtt gaaaccatta atgaaaccaa cttcggcaaa cagttttact
    11101 gcttaattgg aatggatttg tatgatgaag tggcggggtt gagttgggag aatgtggatt
    11161 ttcttgagtc ggagaaattg gttttat
//
LOCUS       DANLUA010000078        11106 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLUA010000078 DANLUA010000000
VERSION     DANLUA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..187
                     /locus_tag="PXB44_001443"
     CDS             <1..187
                     /locus_tag="PXB44_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL5281917.1"
                     /translation="YGEEKGYRIARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYAL
                     EDFVKSIHLESQLRKVG"
     gene            192..488
                     /gene="fis"
                     /locus_tag="PXB44_001444"
     CDS             192..488
                     /gene="fis"
                     /locus_tag="PXB44_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL5281918.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(593..3379)
                     /locus_tag="PXB44_001445"
     CDS             complement(593..3379)
                     /locus_tag="PXB44_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL5281919.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRDHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(3473..5224)
                     /locus_tag="PXB44_001446"
     CDS             complement(3473..5224)
                     /locus_tag="PXB44_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5281920.1"
                     /translation="MFKLKSSFVLLNAALLAACSSNGGSFDVQSAKVESQTQTTPKKP
                     SLQDDNSNARRTVSASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDL
                     TKIPYEEEVKAYGSSADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILR
                     TGAHGYVYYLGIEPAKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPEN
                     SYDINVDDSEKPMGHTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKG
                     NRFSGKANAKNTDDPYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAK
                     KDDSESAFDAFKIKLKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFDDLV
                     TRTIDDKTYRVSVCCNNLDYVKFGTYSEGNNSDTAHQYLVGERTAVADLPTGTVKYRG
                     TWDGVMYSKSGSAGAESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTLEG
                     NLKGNGFAGTAKTGNSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSAET
                     DKDRVSITFGGKRQIEK"
     gene            complement(5645..6292)
                     /gene="rnt"
                     /locus_tag="PXB44_001447"
     CDS             complement(5645..6292)
                     /gene="rnt"
                     /locus_tag="PXB44_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL5281921.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
     gene            complement(6377..6784)
                     /gene="gloA"
                     /locus_tag="PXB44_001448"
     CDS             complement(6377..6784)
                     /gene="gloA"
                     /locus_tag="PXB44_001448"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL5281922.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            6874..7689
                     /locus_tag="PXB44_001449"
     CDS             6874..7689
                     /locus_tag="PXB44_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281923.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            7693..8439
                     /locus_tag="PXB44_001450"
     CDS             7693..8439
                     /locus_tag="PXB44_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281924.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            8625..10400
                     /locus_tag="PXB44_001451"
     CDS             8625..10400
                     /locus_tag="PXB44_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL5281925.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            10418..10612
                     /locus_tag="PXB44_001452"
     CDS             10418..10612
                     /locus_tag="PXB44_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL5281926.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(10609..11082)
                     /gene="folK"
                     /locus_tag="PXB44_001453"
     CDS             complement(10609..11082)
                     /gene="folK"
                     /locus_tag="PXB44_001453"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL5281927.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
BASE COUNT         3313 a         2386 c         2017 g         3390 t
ORIGIN      
        1 ttatggtgaa gaaaaaggtt atcgtattgc acgaaaacat gttggttggt ataccgaaaa
       61 attaaatccg gaatcaaatt ttaaacgcac ttttaataca ctaagttcca caaaagagca
      121 actttatgca ttagaagatt ttgttaaatc gattcattta gaatctcaat taagaaaagt
      181 tggataaaaa aatgttagag caacaaccta cacaaaaccc attaacagta acaatgttaa
      241 atgcacaagc gcagcaggtt aataaaccac ttcgcgataa tgtgaaagca gcattaaaaa
      301 actatttatc tcaattaaat ggtgaagatc caacagaatt atacgaatta gtattatctg
      361 aaatcgaaca cccaatgtta gatatggtga tgcaatatac tcgcggcaat caaactcgtg
      421 cagcaacaat gctaggcatt aaccgtggca cattacgcaa aaaattaaaa aaatacggta
      481 tgggttaatc taaccgaccg ttaaaacacg attttaattc gtatgtaatt agttagagcc
      541 aacaaaaaac gctgaatata ctattcagcg tttttctatt ttattttgcc gtctaaaact
      601 tcatttcaaa tgctaaactg aaatttctcc ccggagcggc aaatcgagtg taattgctac
      661 cccggtcttg gtttactgca ttcacaccgg attggcgcac tgattcccaa gtggaatatt
      721 tacgattagt cacattataa attcctccac gtaaggtgta gtttttataa ttgatgtaac
      781 cggtaatatc atgggtgtac caagaaccgg tcagtttgcc acctaaatca acgcgatgta
      841 tgccgtgatg acgtgtgcct gccacctcat cggcgttttt ggctttagaa taggtggtag
      901 taatgccaat tccccatttt tcttctgggt gatcgtagcc gaatccaagc acatagcgtg
      961 ctggctgaat cgcatctaga ataggatcgt ttacgctatc gagtcttgag tcggttagtt
     1021 tccgctcttt tacttttaca cggttataag caactgatga ataaaaacca tctggcagca
     1081 tagataaaat accgtgtaag tcaaatttag cgactaaatt tatcccgttt agtgctacat
     1141 cttgaatatt atggtagtta tagaaacagt gtgatttttg tattattctt ttacatgctg
     1201 tcgcaatcat atttttatag ttattacgga aatagctcac atcaaggtaa ccaaattgac
     1261 ctttaaagct aactccaatc tcatgattag tcgatttttc tggctctaac tggtggctac
     1321 gttgcgcgcg ttgcacatat tcgttatctt taagcccaat atgatcacgt ttaccataaa
     1381 gttcatagaa tgcaggcact ctaaaaccgt ttgaaatgcg ataagaaagc gatacaaact
     1441 ctgttggttt aagcgtaatc ccaccattcc aagaccaatt tcgataagtt cggctaagtg
     1501 tccacggatc atcagattta aatcgatgtt gatcaaaacg cacccctaat cccaaatcca
     1561 catactcgct tatcactaga tcgcggaagc taacaaaatg gctatcgcct ttaattttac
     1621 gcggttcaca gtttaagatg tcgccgcgtg tattattaca caaagatacc gtttcaatac
     1681 tgcgtggatc gcggcgataa atatatggat tatctaaacg ccctctaccg cggtagctgg
     1741 tataaccgcc tttggtatat tgggcagtca tatccccatg atccataagc gaattaaatc
     1801 gatcaaagcc caaccctaaa ttcagtttgt gggtttgctt aaatacgcct tgaccagcat
     1861 ttagcgcttt atcaaattct aaatgaatga cacgatgctt ttcttggtaa ttattacgct
     1921 cagttcgata catagaccaa gatttatcca aagtcgggcg gcaattttta tctaccacag
     1981 gataatcgct acaatgcaag cgatgtagcc ggctatatag ttcaatatct tgtttatccg
     2041 cactgagttt aatgctatcc aaccagcgat tattctctgg tttatattta taggtgaatc
     2101 ctaaacgacg tttgtggtga cgttcatcaa aaaacttcac atggctatag cgcaacccat
     2161 aagggatcct tggcttgaac actaagccat ctaaaatatt atccccttca taaacatagt
     2221 tcctaagtga taagttaata tcgtcttttg tatagtaagc tggcgtttgc atatcacgga
     2281 tatcgtagcg ttgttttgta tcttctaaga ttgcgccaag ataatgagac gagttaaagt
     2341 gatagccaaa cttcataaaa acagaattac tcttgtaatc taaagggttt ggtgcaatgc
     2401 gatctttacc ggtatattct tgggctgaga gctgctcagt gcgataagga atctgctcaa
     2461 gctgtttgcg ctcttcaggc gtatattccg gaaatgttct cactgggaaa ttatcgcggt
     2521 tcaacttaac ccttgcacga ggagtacaat ctaatgttgg gcaagtatct tccacaataa
     2581 aaaaagatcc tccaggagca ttctgtgcgg aatttgggta aagatcgtag cgattttcaa
     2641 agccggttat acgccgaata ttatgtttta atttatttgc ctcggaatga attttggtct
     2701 ctttaccgtg tcggtgagtt gcaataacaa gtgcttcaaa accacccgcc tcaccagccg
     2761 ctgcgataga ctgtaaaaaa tggctatttt tgctggcata agaggtctta ctatctaagc
     2821 cccaatgctg cccctcttta ataatatcct gcgcatcttt agtacgaaaa ccaatagcac
     2881 cacctagcgc accagagcca tattccgcag aacttgctcc tttgcttaac tcaattgaac
     2941 gaatgttttc atactcaatc tcattaattg caccaccatt agcatctgaa cctagcgtat
     3001 gataatgctg cgcttgtggt agcccatcaa caagtaagct gacacggttt ttatctacac
     3061 ctcgaatggc atagccacta cttgcaccgc gaccttgttc caccaccgaa ataccagggt
     3121 catagcgagt taaatcacga ataccaagaa tttgattttt actcatttcg tgataatttt
     3181 tcactacttt ccccaagcca gttacttcgt tttgacgttc gtgagcataa tggctctctg
     3241 tcacaataac ttcatccaga acagctagat cgttattttc ttctattttt ttattttcag
     3301 tcgcagcacc gtatgaatga gaaagagcaa ataacactgt taaggcaaca ggtgaatagc
     3361 gaaaatgatg atatttcatt atcattagat agggtctcct ttatttaaat tttgcaaatt
     3421 tttataaaaa attgcccgct tgctatctac aaccagcaaa ggggaattat gattattttt
     3481 ctatttgacg ttttccgccg aatgtaatac tgactctatc tttatccgtt tctgcactat
     3541 gtacaatacc acctaattcc gtcgccttag ggccataaaa gcccccttca aattgagtat
     3601 ttatatgcac taccgtgcca ccattcgtac ttttcggatc aagattaaaa ccagaattgc
     3661 ccgttttggc tgtgcctgca aaaccattcc ctttcagatt gccttcaagt gtcagcattg
     3721 ggcgttcttc aacaccatca ttagcaatca gcttgccatt aattttttta ttgacaaaat
     3781 ctacatcgaa tagtgaacga gtaccacttt cgctgttact tggcgattca gcccctgccg
     3841 agccagattt actgtacatt accccgtccc aagtacctcg atattttact gtccctgttg
     3901 gcaaatctgc cacagctgta cgttctccta ctaaatattg gtgagcagta tcactattat
     3961 ttccctcgct ataagtccca aatttgacat aatctaaatt attacagcag actgaaactc
     4021 gatacgtttt atcatcaata gtacgggtaa ccaaatcatc aaatttcatc tcggcaaagc
     4081 tttttttgcc ttccgcaata agtggaatct gcttattgtt aataatcaaa tgtgtcgcat
     4141 tcccgaaagt atccatctca gatttattta aatctttaag tttaattttg aaagcatcaa
     4201 aggcagattc actatcatct tttttagcat ctcgtttgcc agcaaaaaca acaaataccg
     4261 acttatcatc agctaagaat ttaccggcaa gttcttgagc cttcccacca aaaaatccac
     4321 cttcaagtgt cgtagagctt ttaccaaaat aaggatcatc ggtatttttt gcgtttgctt
     4381 taccagaaaa gcgattacct ttaatttttg catcaatatc gtaaattgtt gtaatttttt
     4441 gctctttgct acgactaaca tacccattac gaaccaatgt tccagttaaa gttttattag
     4501 caaaatcagc cgtaaattct cctgtatgcc ccattggttt ttcagaatca tcaacattaa
     4561 tatcgtaact attttccggt gtggcgccaa tattgatgcc tgcggaatta gaaaaatatt
     4621 tactatctct cccttttctc gcatcggtag taaaatccca atatccttta taagtagctt
     4681 tttgagtagg tattgctttt gccggctcta tacctaaata gtaaacatac ccatgtgcac
     4741 ctgttctcaa aatttcttta ggctttattt caggttttgc accagaatgc agaacatagc
     4801 cagaacgaac aaaattaaaa tctctagtat acaacttatg atttctatca tgagtatggc
     4861 taaatccatc agcactacta ccgtacgctt taacctcttc ttcatacgga atttttgtca
     4921 gatctccagt aatctctttt atatcaccaa taggggctac atcttccttc ccctgcggaa
     4981 agagattacg acgcggaatt ttggctgaaa aaccaaaccc cggctgcaat aaagcttcag
     5041 tttcagaagc gcttactgta cgtcttgcgt tactattatc atcttgtaaa cttggctttt
     5101 tgggggtagt ttgcgtttga gattcaactt tggcagattg aacatcaaag cttccaccat
     5161 ttgaggaaca agcagcaagt agcgccgcat taagcagtac aaaactactt ttaagtttaa
     5221 acataatgtc tccttttaat ttataaaatg ataatcatta taatttatta tataaactaa
     5281 tgtaaaggtt tgaactttat tagtttcagg atcatgttaa tcatttcaag gtggaaacgc
     5341 ttgtttttag attagataaa aaataaggag ggtcgaaata gcgggtatac agaaagtgct
     5401 tatagccaaa aaatagtcta ttgttattga atacaagcgg tctattttgt cacatttttg
     5461 ccaaatttgg ttaaaaaacc aaccgcttgt aatcttaact gaagatagat agttttttat
     5521 tttttctaca actctacagg gctggattta ttgcagaatg gtacttgcca ctaactatca
     5581 tcactcccag gataatttat agaaacaata cgagaaattg tgttaataaa tgaaatgaat
     5641 aacgttaatc cgataatggt gggaaaccac ctaaatcttt taaacgattg accattgcac
     5701 agaaaagttc tgccgtgcgc tcggtatcat acaacgcaga atgcgcttgc ttaccgtcaa
     5761 acgggatttt tgccacctgg caggctttta ccaacacggt ttgcccatac ataaaaccgg
     5821 caagggtggc ggtgtcaaac atcgcgaaag ggtgaaaggg atctcgcttg gcattaattc
     5881 gtttaatcgc agcctgtaaa aagccctgat cgaacgctgc attgtgggca acaatcaccg
     5941 cccgttggca gccttgctcc ttcattgctt tgcgaaccat tttgaacatt tccggaatcg
     6001 caatattctc cgcaaccgct cctcttagag ggttgtcaat atcaatgccg ttgaatttta
     6061 atgactcagg gttaatattc gccccttcaa atggcgcaat atgaaaatgg cattgttgat
     6121 ccggcactag ataaccttgc tcgtccattt ttaaagtaat tgccgccaat tccaataagg
     6181 catcagtttg ggcatttaag cctgctgttt ccacatcaat cacaaccggt aaatagccac
     6241 gaaaacgatg ctttaataat ttataatcta tttgttccgt tgtctgttcc atttatctat
     6301 caacctaaaa tacaagcggt ctgatttgca aaatttttta caaatccgac cgcttgaata
     6361 ttcatattcg caaaaattag tttcctaacg ctgctttcgc atctttattc tcaataaatt
     6421 cgattttata accgtccgga tcttcagcaa aggcaatcac agttgttcca cccaataccg
     6481 ggcccggctc acgggtaatt ttaccgcctg ccgcacgcac atcttcaatg gttttgtaaa
     6541 tatcatctac gcctaacgca atatggccat aagccgtgcc tagctcatat ttatccacac
     6601 cccagttata agtcagctca atcaccgcac tttcgctttc atcggcatag cctaaaaatg
     6661 ctaacgaata cttatactgc tcgttctcgc ttctacgtaa cagacgcata cctaacacat
     6721 cggtataaaa cttaattgag cgctctaaat ctccaactcg taacattgta tgtaaaattc
     6781 gcatattttc ttccccctga agttaaaaaa cgacccctat tatggcataa tcttggctta
     6841 aaatcatttt ctttatacct tttgggatta tctatggctc acgatcacga ttcgccgtca
     6901 aaacttgaaa tttcaaccca cgctgcactg ccaatggtat taatgctgtt tattgaaatg
     6961 gctcttaact tctactctgc accgaaaaat tcaattttta tttctcctta cttattggcg
     7021 tttttcgtga cctcattaat cagtttttta gtgctatgga aaggcgaaat ctgccgaggg
     7081 caagccagcc gtttaagctt tattttaccc tttttactta ttttcgccgt agggaatttc
     7141 ctttattcca taggctttac gccaaagcat aacccaatgg cggtggcaag cattgctgca
     7201 gtttttgcct gcttaatcta cttccgcctg ccggaagatc aaaccttata taacacattg
     7261 atgtattgtg gtttggctat atcaagtatt ggtattatcc aatatttagc catttattgg
     7321 tttgaactgc cttcattgtt taacgggatt cgagccaaca atttcgccca agtgctgtta
     7381 ggcattttat tagccggctg gtatttaatg ttagcgaaaa gccgtttaga tgttttcctc
     7441 aaactgttgg tgaaaaccgc ccttgtggtg ctgattctca attattgctg gacagctttt
     7501 gtgctttatc aacatctaca aattatgccg gatatgccat tgctttcatt tatcatctat
     7561 tttgcagcac aatttgtgat tttattgatg ttggcgtggt tattacttgg aaaaaacatc
     7621 aaaaatccga ccgcttggac agttgctacg ctgttagcga ttttataccc tttcgctaat
     7681 gtagtctgat ttatgaaacg ggcattaaaa tttttcggca ttgcaaccgt tctatctgtt
     7741 gtggcaatct actatttttt aatgcccagc cactatcttg ccacgccgat taactggatt
     7801 ttgccgaaag cgtggaaagt tcaactccca caaggcttaa acctgaattg gcaaggctca
     7861 tcacttgctc aatttcaact ctcttatcga gattgcccgc tactaagtgc cgataaaacc
     7921 gatttgggtt ggattgagaa aaaccatctt tctgtcgata aattggatat tgattaccgc
     7981 tgccttgcct tgtttccaca aaatgaacac agcaattccg acacttcact caacgcattg
     8041 ctggcggtca ttcctgatgg cgaagcgact atccacgccc ttaactggaa aaatttgcct
     8101 accgatttca acccaagact tcagcggctt tttgagcaaa cagctattaa actctcactt
     8161 tcaaaaggtg aaattcaggc aactgccgct caacaagcgg tcaaatttgc agcaaatttt
     8221 gcaaatgggc ggttgaatgc cgatttatca tatcaaccga atgaaaaaga acagcataat
     8281 cttaggattt cagcagaaat tgaggacaat tttacccaac tgccgcccac tatttccgct
     8341 gaatatgatt ggcaattatc ttatgaaatc atcgccaata aagccttgca aaagggcgct
     8401 ctattctcag ttggcaaaaa aatgccgcac aaaagttaga aggcaactgg caggttgaac
     8461 ttgccgaaag tgaaaataac aaactggatt tcccttttct gtttgatggg gaaagcctag
     8521 aaattcaaca agggcggttt gattggcact ttacccaaca atttccactg caagggtttg
     8581 ttagcaccaa gctcacaccg aaatcattca accaaaacgg ctttttgcca ctcaaaacgg
     8641 cgattcgtat cagcctgctt tcccaaaaca gttggggtaa aggcaatatt gtaatctcca
     8701 attcagacgg agaaataacc gaaaaagggc tgaatttacc gcttcgtttg acgggtaata
     8761 tcaaacacgg caattttatt ctttacagta gcgtgccact ggattttcaa ggtaattttg
     8821 acgatttaag cgtaaaattt ttgcctagct ccctacttcg tttgaccgga aaagagcggt
     8881 atttaaccat tgaagattta cgttttccac ttgccggaat taaaattgac aagcacggta
     8941 ttcacggacg actgcacgca attttaagag ggcaaagccc tgattttaac aatatcgagt
     9001 tccatttaga cggacaagca caaaatttta aggctggagc cttagatttt ttccaagatc
     9061 ctaaagatgc taacgcagta aaagacagtt ggaaatggcg aatttgggga gcatctatcc
     9121 tcaaagcctt caacagtaaa gtgaatttaa gcggcagagg gcaatggcat aaacagcttg
     9181 tgcggttgga agaactcaaa ggcgatttag ccaccgtaaa gttagcagat acaaccattt
     9241 ctaaaatcac gcttaacaat acgcaagcta ttcgatttaa tgtcaaaaaa ttcacccttg
     9301 acggagcggt aatgttgcaa tcacctgaga ttgccttcag ttatggcggt gtgctaccca
     9361 aaccacgagt gaatctcgcc tttgatggtg aagtcgaaaa actacgtttt aaaggagcgg
     9421 tgaataccac cgaactcggc ccactcaaac tgttcgcccg ccgccaatta agccaagatg
     9481 ccagccaaat tatcggcaaa ctttattggc cggagcaatc tgctcaaggc ttccagccgc
     9541 tttttccgtt tcgtagccaa tgggtgatca acaacggtac aattcggggc gaaacggcgt
     9601 ttagtgccgg cagtaaaaac gggctgattg ccggtgggca tttagccatt cgtaacggcg
     9661 gtttatcttt acctaatggc gaagcaaaag ggattgaatt ttcgcttccc taccgctacc
     9721 aaaacggacg ctttcatttc ggagccaaaa aaccgcttga tgtaaaaatt gcccaaatca
     9781 aactggggga tttagcatta gataacgctt caatgaaact caacggttac tacccttata
     9841 ccaaagctaa gccattgaat ttaaatcaat tatcctttga tttgtttggc ggtaaactga
     9901 atgtaaaacg ctttgcctta ccacaaaccg aactggctta cctgaattta gagcggattg
     9961 actttgagca aattttgcaa tttatgcaat atcagcaaat tgatctcaaa ggcaaagcca
    10021 acgctatctt gccgttttgg ttaagcggaa aaccttgcta tatttgcgat ggcttactga
    10081 cacaggcgga aacttcctat ttaaccttta cccctgaatt gttgtcagaa atgcagaaaa
    10141 gtggttacac cgagcaaatt ttgttacatt tagtagataa aagcacgatt aacgattttc
    10201 gcagtctgat taatgtcggg ccgaaaggcg atttagtatt agacggaaaa ttaaaattat
    10261 cttccaatca aaataaatca aaagtgaatt taaactataa tcaccgagaa aatctgtttg
    10321 atttgtggca cttaattaac tatggctcac aatttgaaca aaaattagaa aattacctct
    10381 atcaaaaact ggaacgatga taaagaatgg aataaatatg aaaccggcca cattttattt
    10441 tgcaatattt ttactgaatt tgaccgcttg tacgccgaaa gtacagcttg aaacgccaag
    10501 tgaaggcatt acgattaata tgaatgtggt cgttgatcac cgcattgatg tgaagatgga
    10561 cgagaaatcc cgagcggtga tcaccaaaag tgagaacaaa acgaaaaatt agctgaagat
    10621 aatcatttga tgatctttaa attgtgcata aatatcggct aatttttccc ctgtcggtag
    10681 tactaaatca ggcgttaaat caaacatcgg cacaatcaca aattcccgat ttttcatctc
    10741 gatatgcggc acgttcaaac gctcggtttg aatttgctga ttgccgtaca aaataatatc
    10801 cagatctaaa gttctctccc cccaacggcg tacacgcact cttccctgct cattttcaat
    10861 gctttgcaat ttatccagca atgcagtggg cgataggtcg gtactaaatt ttgctaccgc
    10921 attgacataa tcaggctgat cttgcggtcc gacaggcttt gagccgtaga acgggctgat
    10981 ttcaaactca tttgcaaatt tttttaattt ttcgaccgct tgtttaagct gctcaagcgg
    11041 attctcaagg tttgatccta aggcgatata gacggtttgc ataaaatata cttacaaata
    11101 taacaa
//
LOCUS       DANLUA010000079        10974 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLUA010000079 DANLUA010000000
VERSION     DANLUA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            25..957
                     /locus_tag="PXB44_001454"
     CDS             25..957
                     /locus_tag="PXB44_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5281928.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            959..1756
                     /locus_tag="PXB44_001455"
     CDS             959..1756
                     /locus_tag="PXB44_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5281929.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            1843..2064
                     /locus_tag="PXB44_001456"
     CDS             1843..2064
                     /locus_tag="PXB44_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281930.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            2247..4172
                     /locus_tag="PXB44_001457"
     CDS             2247..4172
                     /locus_tag="PXB44_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL5281931.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            4246..4611
                     /locus_tag="PXB44_001458"
     CDS             4246..4611
                     /locus_tag="PXB44_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL5281932.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            4613..5299
                     /locus_tag="PXB44_001459"
     CDS             4613..5299
                     /locus_tag="PXB44_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL5281933.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            5383..7146
                     /gene="msbA"
                     /locus_tag="PXB44_001460"
     CDS             5383..7146
                     /gene="msbA"
                     /locus_tag="PXB44_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL5281934.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            7235..7867
                     /gene="lolB"
                     /locus_tag="PXB44_001461"
     CDS             7235..7867
                     /gene="lolB"
                     /locus_tag="PXB44_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL5281935.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            7855..8709
                     /gene="ispE"
                     /locus_tag="PXB44_001462"
     CDS             7855..8709
                     /gene="ispE"
                     /locus_tag="PXB44_001462"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL5281936.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            8735..9685
                     /locus_tag="PXB44_001463"
     CDS             8735..9685
                     /locus_tag="PXB44_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL5281937.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            9763..10773
                     /gene="pyrD"
                     /locus_tag="PXB44_001464"
     CDS             9763..10773
                     /gene="pyrD"
                     /locus_tag="PXB44_001464"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL5281938.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         3160 a         1973 c         2513 g         3328 t
ORIGIN      
        1 gatttcatta actataataa acaaatggac agtcttattc attttttgag tgccttttta
       61 actaatatca cttggattgg ggcgttagag ctggggttaa tttattccct tgttgccctt
      121 ggggtattaa tttcttataa aattctggat tttccggatc tgaccgcaga cggcagtttc
      181 cctttaggag gggcggtgtg tgtgatcagc attttgaatc aagttgatcc ttggctggca
      241 actttatacg gcacgcttgc cggagcagta gccggtatgg ttactgcaag tttgcatatc
      301 ggctttaaaa tcgaaaaatt gcttgccagt attttaatga tgatcgcact ctattcgatt
      361 aatctacgca ttatgggaat gccgaatatt gctattttag gcgaaaccac gatttacgat
      421 gtaatgccga ttgaaagcga tctacagtta gccttagtac gattagtgat ggcattgctg
      481 gttgttatta tcgtaaaagg tttgtttgac ctcttttttg cgaccgaaat cggtttggct
      541 gtgcgggcaa ccggtactaa tagtcgaatg gcgaaagcac agggtattgc gattaataaa
      601 atgacgattt tagggatggc gatttctaat gcgttaattg ccttaggtgg tgctttgtat
      661 gtgcaaagca acggtggtgt agatatttcg attggggtag gaactatcgt gattggcttg
      721 gcggcggtaa ttatcgggga agcaattttt tcggtaaaac ggattatttg gcttaccttt
      781 gcggtgatta ttggctcaat tctttatcgt ttcttcatcg cactagcatt aaataacgaa
      841 actttaaacg gcattggctt tggcccgcaa gatctgaatt taattacggc gttattggtg
      901 gtgattgtat tagcggttcc tcgaattaaa cacaaattgc tcggtaagcg aggttaatat
      961 gattgaactc aataacttat ttattacctt caacaaaggg acagcaattg agaaccctgt
     1021 gctgagaggc ttatccctaa aagttgagca aggcgaattt gtgtcggtaa ttggtagcaa
     1081 tggggcgggt aaatctacct tattaaatgc gatcagtggt gattgtgcgg tggactccgg
     1141 tgagatttta attcaaggaa ataaggtaaa ccaatccacc acttggcaac gggcaaatca
     1201 agtcgcccga gttttccaag acccgatggc tggcacttgt gaaagtttaa cgattgaaga
     1261 aaatatggca ctggcttacc aacgtggacg taaacgtggg ttgggctttg cgatcaaacg
     1321 ccaaatgcga gagttattca aagacaaact tgctttgtta gggctaggtt tggaaaatcg
     1381 gcttactgat caaatggggc gtttatcagg cgggcaacgc caagcggtga gcttgctgat
     1441 ggcatcactt cagccgtcta atatcttatt gttagatgaa cacaccgcgg cactggatcc
     1501 gaaaaccacc gattttgtgc ttgaacttac caataaaatt gtaagagaac agaaattaac
     1561 cacgctaatg gtaacgcatt caatgcggca agcccttgac tatggcgata gaaccgtgat
     1621 gttacaccaa gggcaagtgg cgtttgatgt ttcgggcgag caacggcaaa aaatggacgt
     1681 gccggatctg ttacaacttt tccaacaaag tcgccacgag cagttgagtg acgatggatt
     1741 attactcggc aattaatttt gcgatctgca tcgaaaaaat gaatttctcc ttttgttttt
     1801 ggctgtcaaa ttttaaataa taagcccaaa aggaggtatt caatgttaag cattttcaaa
     1861 acaccggtag agaaagaaac gctagacgat tgggctaaaa tttcggttga tgtagcgaaa
     1921 gttgcgattt tggcaattcc tgttatactt tttggcaaag aaccaacgtt gattaaattg
     1981 actaacttag cattattggt attgagtatt tatgtttttt tgacgcttgg gcgaaaatta
     2041 cggcagctaa aggaggtgtt gtgatggaac taggctggaa tttaaccata ggtttgctaa
     2101 ttttaaacgt ctttgcgttt gcttggcttt atattattaa ccggatttct aaagaacaat
     2161 tatccaaaaa tcaataatat ttaatttttc acaagcggtt gtttttgcaa aatattttgt
     2221 aaattcgacc gcttgcctgt ttttaaatga caaaatacga cgataaaatt cagcatgcat
     2281 ttagcgaaaa tgggttgctc agccaaaata tcagtggatt tcgaccacgc caagcacagc
     2341 ttgaaatggc tcaagtggtc gcaaaagcag taaaatttgc gacacctgtt gtggtagagg
     2401 cgggaaccgg cacaggcaaa acctttgctt atcttgtgcc ggcactactt tcaggcaaga
     2461 aaactattct ttcgacaggt tctaaaaacc tgcaagatca actttttaat cgagatcttc
     2521 ccactattca aaaagccctg aaatacaaag gcaaaattgc gttactcaaa gggcgtgcga
     2581 actatttgtg tttagagcgt ttagatcaag tgaccgcaat gggcgtactt ggggataaat
     2641 ccgttcttac cgatttaaaa aaagtcagta aatggcaaaa tgccactaaa acaggggatt
     2701 taagtgaatg tattaccatt gccgaagata gcccgattct accgcagtta gttagcacca
     2761 ccgagagttg cttaggctcg gactgcccgc attttaaaga ttgttatgtg gtacaagccc
     2821 gccgtaaagc gatggaggca gacgtagtgg tggtaaacca tcacctattc tgtgctgata
     2881 tggcagtaaa aaagaccggt tttggtgagt tgatccctga agctgagctg gtgatctttg
     2941 atgaagctca ccaactgccg gatattgcca gccaatattt tgggcaatcg ctttcttccc
     3001 gccagttatt tgatatttgt aaggatacca atattgttta ccgcactgaa ctcaaagatc
     3061 tgtctcaatt aggcaaagcg gctgatcact tgacgaaagt agtgcaagat ttccgattat
     3121 tgatgggcgg agaagggcag attcgaggca atttgcgaga gttatttcgt gataaaaaag
     3181 tggtcgatgg cttggtaaaa gtggctgaaa atattgattt tttaagtgaa gtggcgaaaa
     3241 aatcgctggg gcggtcggaa acgttggata aaattttcga gcggttagca gaagttaagg
     3301 ttcaacttaa aaaattaatg gatactagtg tgacaggcta ttgttactgg tatgaggcga
     3361 atggacgatc ctttgggctg catattacgc cgcttatggt ggcagataaa tttggtgaac
     3421 agttgaagaa ccagcaaatt ggttgggtgt ttacctcagc cacactcgaa gttgggggca
     3481 aattcgatca tttttgtcaa cggctgggca tcgaaaatgc ggagcagttg gtgttacaaa
     3541 gtccgtttga ttaccaacat caatctttac tctgtgtgcc ccgctacttg ccggatacca
     3601 ataaatccaa tactttaact gctctcgggc aattattaaa accggtgatt gaggcgaata
     3661 aagggcgttg tttcttgctt tgtacttcct attatatgat gcgaggttta gccgactttt
     3721 tacgcgaaca cagttcgctg aatgtgttat tgcaaggcga aaccagcaaa agccgtttat
     3781 tggaaaaatt tgtgaaagaa gatcacagcg tgttggtggc gacccaaagt ttttgggagg
     3841 ggattgatgt gcgaggcgat gatttatcgt tggtgattat tgataaattg ccgttcactt
     3901 caccggacga accattactc aaagcccgaa tggaagattg taagatacag ggcggtgatc
     3961 cgtttaacga tattcaaatt cccgaagcgg tgattaccct caaacaaggt gtagggcgtt
     4021 taattcgtga tgtgaccgat agaggtgtag tgattatttg tgattcccgt ttggtgatgc
     4081 gaaattatgg gcaaactttc ttgaaaagtt taccgccgtc tagacgcact agagatttgg
     4141 ggaatgttat tcaatttttg aaacaaaaat agggttacaa gcggtcagat ttctgcagat
     4201 ttttgcaaaa aaacggataa aaataaccgc ttgttgagga gttatatgtt gtctacattg
     4261 ctgcgtatgg ttgcttcttt ggcattgtta tttgtgatgc tttatttagg caagttaatc
     4321 gtttatctat tccccatcgg gatcccggat agtattttag gtatgcttat cttattcggc
     4381 tgcttggtta tcggcattgt taaagtggaa tgggtgatac cgagcgcgaa accgctcaca
     4441 cgttatataa cactattttt ccttccgatt tgtgtcgaac tcatcgaaca tcttgagctg
     4501 ttacgccaaa atctgaacac ttttgtatta gcaacggcat taagcacctt agtttctgtg
     4561 gtcgcgattg gcgtgtttgc acagtggatt tttcattaca agagagggta aaatgagcta
     4621 tctttacact gtgttgacgg taatcatgtt tattgccggt ttgaaaatca gccaaaaact
     4681 caagagcagt tttctgaacc ctttcttaat tgcactcgct ttgctaattg gtgtattact
     4741 gatttttaat atcccttacc aagactatta tcaaggtaat tcccccttga ataacttact
     4801 tggcgtttct gttgtggcac ttgcttaccc tttttatgaa caattacccc atatccgtaa
     4861 aaaatggcga cagattgtcg tgattttgct gtttagtacc atcattacga tgataaccgg
     4921 gcttgggttt tcattattat ttggggcaag ccaagagatc ttagccgcca ttttgcctaa
     4981 atcggtatca atgccaatgg caattattat tacgaatgaa attaatggaa atgttgcaat
     5041 cgcagcggtt ggggtgatga tcgcaggctt agtgggatct attttaggct ttacgcttct
     5101 gcgcctggca aaagtaagaa atgtgatggt gattgggtta agtatgggag ctgcttccca
     5161 cgcattaggc acggcaagat gcttagagca tagtatgaaa gccggtagct atagttctat
     5221 cgcattagtg gcttgtggtg tgctgtcttc tatatttgca cctttaatat ttaacgttat
     5281 cgtcacgtta tgttactaaa aacagtgaaa tctcaaacct tatttggtaa ctggctgaaa
     5341 tcaaggtaca ataccgtgat ttttcaatga aaatagagaa ttatgcaaga acaaataaca
     5401 caagaaaaag atctttctac gcttaaaacc tttaagcgtt tatggccgac tatcagcccg
     5461 tttaaagcgg gtctgattac aggtgctgtc gctttagtat taaatgcgtt agtggattct
     5521 agtcttatct atctcctcaa acccttgcta gatgaaggct ttgggcaagc taataatgat
     5581 tttctgaaat tgatggcggt gcttgtagta gtttttattt tagtgcgcgg tattaccaat
     5641 tatattgcca gttactgttt agcttgggtg tcaggcaagg tggtaatgac attacgccgc
     5701 catatttttc aacatttaat gtatatgccg gtaagctatt ttgataacaa cccgaccggg
     5761 cgtttgctct cacgagtgac ttacgatacc gaaatggtgg cgagttctgc ttctcattct
     5821 ttagtgacta ttgtgcgtga aggggcttat ctgctgtcat tgtttgtggt gatgatctat
     5881 accagttggc agctttctat tgtgttattt gtgatggctc ctatcattgg ggtactgatc
     5941 agtttcgttt caaaatattt tcgtcaatta agtcgcaata tccaaagttc aatgggggag
     6001 cttactgtca cgacagaaca aatgcttaaa gggcataagg ttgtactttc ctttggtggg
     6061 caggaaattg agaaagcacg ttttgatcga gtcagtaatg atatgcgtcg taaagggatg
     6121 aaagtcgttt ctgctgatgg catttctgat ggtgtggtac agcttatcgc ctcttttgcg
     6181 ttatctgctg tgctttatgt tgcgaccttc cccgaggtga tgagtgagaa tttaaccgca
     6241 ggttcattca cagtagtgtt ttcttcgatg atggcaatgt tacgtcctct aaaatcactc
     6301 accaatgtga actcgcaatt ccaacgtgga atggcagcct gccaaacgtt atttgaattt
     6361 ttagatttaa aaactgaaaa agatcacggc ttaatctccg ttgctaaagc tgaaggcaat
     6421 gtggaattta aaaaggtaag ctttagttat gaaggtaaag aggaaaaagc actcgataat
     6481 atttctttca ccattcctaa aggcaaaaca gtagcattgg ttgggcgttc cggctcgggt
     6541 aaatcaacta ttgccaattt gcttacccgt ttttatgatg tgactgacgg tcaaattttg
     6601 cttgatggaa ttaatattca agattaccgc ttaaaaaact taagagaaca atgctcggtg
     6661 gtttctcaac aagttcattt gtttaacgat actattgcta ataatattgc ttatgctgca
     6721 atcgacaaat acagccgaga agaaattatt gctgctgcaa aagctgcaca cgcaatggaa
     6781 tttatcgaaa aattggaaaa tggcttagac actgtaattg gtgaaaatgg cgcaagtctt
     6841 tccggtgggc aacgccaacg cttggcaatc gctcgtgcat tactgcgaaa ttctcctgtg
     6901 cttgtgctgg atgaagctac ctctgcatta gatacggagt cagaaagagc gatccaatct
     6961 gcacttgaag tgctacagaa agatcgtacg gtgttggtca tagcacaccg gttatcaacg
     7021 attgaaaaag ccgatgaaat tttggtagtc gatcacggaa atattattga gcgtggcagc
     7081 catacggaat tgttagcaca aggtggggct tataaacaac tccatagttt acaattcgga
     7141 gcctaatttg taggggagaa atatgtttct ccccaattaa tgataaaaac agggcgaaaa
     7201 atatttcgtc cctacaataa tttatttgga tactatgaaa aaactgacaa aacttttatc
     7261 tcttagtgct ttactggtct taaccgcctg taattcggta ctaaatgcct caacagaggt
     7321 gcaacaacca acagtacaaa ttccgcatga cgatccacaa tggcagcagc atttagcgaa
     7381 actgagccaa attcaaggct atactgcaac ggggcaattt ggctatattt cgcctgaaga
     7441 gcgtttttct tcccatttca actggcgtta tactaatcca atccaatttg ggttagagct
     7501 ttcctcgaat ctctcgacta aatcactgaa attagagcgt aataaacgag gcttaacggt
     7561 ttcggacaat gagggtaata gccgttctga ccgtgatgtt gatgcgttaa tgcaagagat
     7621 tatcggtgtt tcgtttccta tcgatcagtt tgcctattgg gtaaaaggct tgcctgaaaa
     7681 agatggcaac tatattgtga atgaaaaacg ccagctgtcg caatttagtt atccgcttaa
     7741 tggcacgctt tggaaagcaa gctatgtgga gtatcacgaa gatcgtgtac caaacttacc
     7801 gaagctgatt gtgcttgaaa acggcactca aacgttgaag atccgcattg aaaaatgggc
     7861 gtattaatga agaaagtcac cttacccagc ccggcaaaat taaacctgtt tttatacatt
     7921 accaataagc gggcagacgg ttatcacgag ctacaaacgc tgtttcagtt tttagatttt
     7981 ggtgatgaaa ttgagattga gatcacagaa agttcagagg ttgaattaat taatccggta
     8041 gaaggggtgg caactgagga gaatctcatt taccgagcag caaaactttt gcaaaaaacg
     8101 accgcttgta ggcaaggctg ccgtattggg attaaaaaaa acctaccgat gggcggaggt
     8161 gttggtggtg gatcttctaa tgctgcaaca gtattagtcg gtttaaatca cttgtggaat
     8221 accgagcttt cattggaaaa attggcagaa ttaggcttaa gtttaggggc tgatgtgccg
     8281 atatttgtta gaggtatggc tgcttttgcc gaaggcgtgg gggaggtgtt aactccttgt
     8341 gatccacaag aaaaatggta tgtagtgcta aaaccagaag tgtcgatttc aaccgcagcg
     8401 gtgtttaccg acccgaattt acctagaaat acgccaaaac gccccttagc cgaattatta
     8461 tctttaccgt gggaaaacga ttgcgaaaaa gttgtacgag aaaagtattc tgcggttgaa
     8521 gatcttatac aagaattgtt acaatatgct cagtttaggc taacaggcac aggtgcttgt
     8581 attttcgccg aatttgaaac aaaagcagaa gccgagcagg tttttgcaca taaaccggaa
     8641 gccgtcttcg gttttgtggc gaaagggcaa aatatttcgc cattacatca aatgttattc
     8701 caaatctaac catcttaacc ttaaggatca atccatgaca gatattaaac tgtttgcggg
     8761 caacgcaact ccggaacttg ctaatcgtat tgcaaaacat ctttatacct cattaggtaa
     8821 tgcaacagtt ggtcgtttta gcgatggtga aattcaagta caaatcaatg aaaatgtgcg
     8881 tggaggcgat atttttattg tgcaatccac ttgtgcacca actaatgata atttaatgga
     8941 attgatcgtg atggtagatg cgcttcgccg tgcatcagct ggtcgtatta ccgcagtgat
     9001 tccgtatttc ggttatgcac gccaagacag acgcgtgcgt tcggcccgtg tgccaattac
     9061 cgcaaaagtg attgctgatt tcttatccag cgtaggggta gatagagtct taacctgcga
     9121 cttacacgca gagcaaattc aaggtttctt tgatgtgccg gtagataacg tatttggttc
     9181 ccctgtgtta atcgatgata ttttgaaaaa atccgatttg gtcaacccta tcgtggtttc
     9241 tccagatatt ggtggggttg ttcgtgcaag agcggtggca aaattattaa atgataccga
     9301 tatggcaatt attgataaac gtcgtccaaa agcgaatgta tcacaggtaa tgcacattat
     9361 cggtgatgtg gctgatcgtg actgtatttt agttgatgat atgatcgata ctggtggtac
     9421 tttagtcaaa gcggctgaag cgctgaaaga acgtggcgcc cgtcgtgtct ttgcttatgc
     9481 cacacacgcg gtattttcag gcacagcagc tcaaaattta gcgaatcctg ctttagatga
     9541 aattgtagta actgatacta ttccactgag taatgagatt aaagcattaa ataaagtacg
     9601 tgttttaaca ttatccggta tgttagcaga agcaattcgc cgtattagca atgaagaatc
     9661 catttcagca atgtttgatg cctaatttat gagaaaaaac ccagtcgaaa gattgggttt
     9721 ttcatatctg ttcactacta tatttagttt aagataattt gtatgtattc cctgattcgt
     9781 aaattacttt ttgcactaga tacggaaaaa gcacatcaat tctctattca ggcacttgct
     9841 ttagccggaa aatcgccttt ttcgattctg ccaacacccg ataatccgac ccaagtaatg
     9901 ggactcacgt ttaaaaatcc aatagggctt gcagccggag cggataaaaa cggtgaagcg
     9961 atagatggtt ttgggaaatt aggatttggg tttattgagg ttgggactat tactcctctc
    10021 gctcaagacg gaaaccctaa accgaggcaa tttcgtattg tagatgcaga gggaattatt
    10081 aaccgcaatg gatttaataa taaaggcgtt gatgttttag tagaaaatgt aaaaaatgca
    10141 acctatagtg gtattttagg tattaatatt ggaaaaaatg cggttacacc aattgagcat
    10201 agctttgatg actaccaaat ttgtttgcgt aaggtttacc aatatgctga ttatgtggcg
    10261 gtgaatattt catcacccaa taccaaaaat ttgcgcagtt tacaatatgg tgaggcatta
    10321 gatgatttgc tgcgaaacct caaaaatgag caggcacaac tttcgcaaaa aatggggaaa
    10381 tatcgaccgc ttgttttaaa gattgcgcct gatttaaccg atgaagaaat cgtttctgta
    10441 gccgatagtc ttattcgtta ccaaattgat ggggtgattg ccggcaatac taccttgtct
    10501 cgtgaaagta ttgccggatt accgtattcg gaacaactgg gtggattaag tggcaagcca
    10561 ttacactcat taagtacaag gttaatttca aaattatctg ccgagttggg cgagagaata
    10621 ccaattatag gttgtggggg agtccattcg ttagagagtg ggcaggagaa agtgaatgca
    10681 ggtgctagtt tattgcaagt ctattccgca atgatttacc aaggaccatc attaattcaa
    10741 accttagcca aacaccttca gctgaacagt tagggataga tttctagtta aaaatgattg
    10801 gttaggttta tttctgtcat cttagtggaa tagatggcgg tattagctat ctatcaaaaa
    10861 tagacaaaat tgcctatttt tgctatcttg cactgcaaat ttatctacaa aatttaaaca
    10921 aattttataa aattatataa aaatcgttta aaatcaaata gataataagt ttta
//
LOCUS       DANLUA010000080        10874 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLUA010000080 DANLUA010000000
VERSION     DANLUA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            47..604
                     /locus_tag="PXB44_001465"
     CDS             47..604
                     /locus_tag="PXB44_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL5281939.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(645..1613)
                     /locus_tag="PXB44_001466"
     CDS             complement(645..1613)
                     /locus_tag="PXB44_001466"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL5281940.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(1739..2902)
                     /gene="galK"
                     /locus_tag="PXB44_001467"
     CDS             complement(1739..2902)
                     /gene="galK"
                     /locus_tag="PXB44_001467"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL5281941.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(2912..3958)
                     /gene="galT"
                     /locus_tag="PXB44_001468"
     CDS             complement(2912..3958)
                     /gene="galT"
                     /locus_tag="PXB44_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL5281942.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            4171..5022
                     /locus_tag="PXB44_001469"
     CDS             4171..5022
                     /locus_tag="PXB44_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL5281943.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(5108..6193)
                     /locus_tag="PXB44_001470"
     CDS             complement(5108..6193)
                     /locus_tag="PXB44_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5281944.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(6215..7195)
                     /locus_tag="PXB44_001471"
     CDS             complement(6215..7195)
                     /locus_tag="PXB44_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5281945.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(7298..8396)
                     /gene="prfB"
                     /locus_tag="PXB44_001472"
     CDS             complement(join(7298..8320,8322..8396))
                     /gene="prfB"
                     /locus_tag="PXB44_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL5281946.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            8652..9560
                     /locus_tag="PXB44_001473"
     CDS             8652..9560
                     /locus_tag="PXB44_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL5281947.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            9861..10502
                     /locus_tag="PXB44_001474"
     CDS             9861..10502
                     /locus_tag="PXB44_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL5281948.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            10669..>10874
                     /locus_tag="PXB44_001475"
     CDS             10669..>10874
                     /locus_tag="PXB44_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL5281949.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT         2991 a         2295 c         2380 g         3208 t
ORIGIN      
        1 gattaggttc ttggcgtgtt cttgcgctga cacaatagga gaaaaaatga aaaaattatt
       61 cgctcaagcg gttatttttg gggctttttt tgcaagttcc gcttttgctc acaatgtgca
      121 attaaatcaa gcattacctg ctgtttcggt tgcaaaagac ggggaattgg cagtggcagg
      181 tggaaaggtt agctataaaa attggcattc atcatctctg gcaggtaaag tccgtgttat
      241 tcatcatttt gccggtagaa gttcggtgaa agaaaaaaat gaagatttga tgacagcaat
      301 taaaaatgcc ggttttgatc gctcgaaata ccaaacggtg acgattgtaa atgctgatga
      361 tgcgattgtc ggcacagggg tatttgtgaa aaatagtgtg gaaaacggca aacttgacaa
      421 tcctcacagc caagtagtgt tagaccagca aggcagtgtg aaaaaagcgt ggggcttaaa
      481 agagaaagag agctttattg cagtgctgga taaatcgggt aaggtacaat ttacctctga
      541 gggcaaactc tctgcaaaac agattcaaga agtgattggg ttggtgaaaa cattaacgca
      601 ataaccagtg tttttatggg cggattttgt atccgcccat cattctagat aataaatcga
      661 taacttatcg tgtgatgata ctcttctcct gcttttgtta tcccgccaaa ttggagtaat
      721 tcaggctggt ttagtgaatt tgggaaaaac tcaggctcta atgctacacc agcgtaatct
      781 tcatattcgc ctttagtcaa attaggttgt ccttttagcc agttgccggt gtaaagttgt
      841 aatgctggca tggaggtgcg gagttccatt tttaagtctt ccacggctaa acaagcggtc
      901 ggattttcac tattttttgc aagatgaaag gagtgatcgt aaccttttac cgctttttgg
      961 tcgtcatcag ccaataaatc ttgcccaatt tttttagcat tgcggaagtc aaaactggta
     1021 ctctccactt ttttcagtgg agcattagga ataccgctgg catcaaccgg taagtatccg
     1081 tctgcattaa ttatcagctc gtgatctaaa atcgtctctg aaccttgcaa attaaaatag
     1141 gcgtgattag ttaagcaaag cggtgtatct tggttagcaa cggcatcaaa gctgatttga
     1201 agctcattct ctgttaagcg atactccaca aaagctttca cttcgccgcc aaatccttcc
     1261 tcgccatctg caaaaaaata actaaatttg accgcttgtt cgctttggct ttcaagttgc
     1321 cacactattt tatctgcccc aagctcgcca ccgtggagat tatgttcacc gttattttta
     1381 gcgagctggt aggttttgcc gtttaagcga tattcggcat tggcaatgcg gttagcgtaa
     1441 cgcccaatgg tcgctccaaa gaaagcggtc tgtttttgcc aattttttgc agacgtggtg
     1501 actaatactt cccgttcctc gttcggcatt ttgacaatgc acgaaagcca agaagcccca
     1561 acatcagaaa gggtaatgcg taaaaacgca ttttcaaggg tgaataattt catcggttga
     1621 ccttaattac ctagcacggc atagccgtac taggttaagc ataattcagc cctgctaggg
     1681 ctgaggttat ttattttaga gtagcggagc tacttcctta tgcttaaccc ggtacgcttt
     1741 agcgtgccgg gcattctcgc accccatcgg aggcagtaca aacgtggaag gtttctttaa
     1801 tgccggtttg cttttcgtaa ttgttagcaa cggcttggcg gagatcttcc actttgtcat
     1861 ttggcactag gcacacgaca caaccgccaa agccgccgcc agtcattctg gctccgccgt
     1921 ttttaccgat agcttgttgg gcgatttcgg ctaagcaatc cacttcaggg caggtgattt
     1981 caaaatcatc acgcattgag tcgtgagatt gagccatcaa ctccccgagt ttgaccatat
     2041 cattcgcttt taaggcctct actgcttcca gaactcgttg attttcgctg atgatatgtt
     2101 ttgctcgttt atacgccagc tcattctctt cactaagttc tgaggctcgt tcaataaatt
     2161 gttccgctgt gacatcacgc aattttgctt tgccgaagaa gtgggcggca aattcgcact
     2221 gttcacggcg actgttgtat tcgccggtaa ccaagtcgtg ttttacgttg gagttaataa
     2281 tcgcaatcga atagccttgt ggcacagggg ttggggtgat ttctaaacta cggcagtcga
     2341 tcatcactaa atgatcttgt tgcccgagag cggaggtgag ctgatccata ttgccgcagt
     2401 tgcagccgac aaatttattc tcagcctctt gcccgattaa tgcgatttgc ggtaaagaaa
     2461 gcggtaaatc gccgagtaat tgagcggttt taccaatcga cacttccaat gcggcggaag
     2521 aacttaaacc tgatgacatc ggcacatcgc tggtaatggc taaatctgct ccttgtttga
     2581 attcggggtg tttggcttga ataaatttca ccacgccacg cacataattc gcccattttt
     2641 gctcgcttgg ctcaatcgcc tggcttaaat caaattcatc ttgttggttg agatcaatgg
     2701 cgaacacccg ccatttgttg tcactacgtt tggcaaagct aaccgctgtg ccgtaattga
     2761 tggcacaagg cattacaaac ccatcgttgt aatcagtatg ttcgccgata atattcactc
     2821 tgcccggggc aaataccgtg cgttctgctt tataaccaaa atgttgttca aattgttgca
     2881 aagcgatttc ttgtggtttc atttcaattc cttatttatc tttatagtgt ttggtatcac
     2941 ttactgcatt taaacgttcg gcagcttgtt ccggggttaa atcacgttgg ctttctgcca
     3001 tcatttcata accgaccata aatttacgca ctgttgccga acgcaagagc ggcggataga
     3061 agtgagcgtg taattgccag tgagcatttt ccttgccgtt aaacggagcg aagtgaaagc
     3121 ccattgaata aggaaagctg atgttaaaca gattgtcgta acgggtggtg agctttttca
     3181 atgcaagggc taaatccgct ttttccgctt cgtttaaatc ggtaatggat ttgaactgct
     3241 tggctttcgg caacagcaag gtttcaaacg gccaagccgc ccagtaaggc acaaccgcaa
     3301 tccaatcttc ggtttctgcc acaattcgct cttgtttgtc taattcacgt tgtgcgtagt
     3361 caagcaacag tggcgagcca tattgagcaa aatatttcgc ttggcattcg tcttcaatcg
     3421 caatttcatt aggtagaaaa ttacttgccc aaatttgccc gtgtgggtga gggttagaac
     3481 accccatcat tgagcctttg ttctcaaaaa tttgcaccca ctgataatgc tctttcagct
     3541 cattcgcttg ctcttgccaa actgttacca catcagtaat ttcctcgatg gaaagttgcg
     3601 gtaaggtttt actatgatca ggcgaaaaac agatcacacg gctttcgcct tgtgtatgag
     3661 aaatctgaaa cagcggatca tcattcggta ttggtgccgg tgtgtctggt agcagggcag
     3721 agaagtcgtt tttaaacaca aacggcttgg tgtaattcgg gttttgttcg ccggtaatcc
     3781 gcttattgcc ggggcagagg taacaacttg ggtcgtagct cggtttttca tcttgcacga
     3841 tttcttcttg ttgcccttgc cacgggcgtt ttgcccgatg cggagagact aaaatccatt
     3901 gattttttaa tggattaaaa cgacgatgtg ggtgatcgtt gaggacaaat tcgctcatat
     3961 aaaactcctc gaaattagtt atgtaaagag tatagcgaaa gtcagacaaa atgactgtga
     4021 catagctcac aaaataatga tgtcaagcgg tcaaatttca gaaaaatttt gcaagttttc
     4081 ctccattttg cgatttaagg tagcttacgt ggaattgaat gtggcttttg gtagaataga
     4141 tgaatatgca agatattaag acaaaaatag atggaaagac tgaataaatt tgatgcttgg
     4201 ttaatgaatc ataaacctaa tgctaggtta agcggagcga gtcgtttgtt gacagagttt
     4261 tggttctttg ggttaaaaga agctagagcc tgtttatttg cgggattatt ttttattgcg
     4321 atgttttcgg taccaaaaac gggattatgg ggcttacctc gttatgatat tttgctgatt
     4381 tttgccatag tggtgcaaat tgcaatggtg aaactggggc tggaaacgtg ggacgagcta
     4441 aaagcaataa gcctatttca tttggttggt tttgtgttag aagtctttaa aacatcgagt
     4501 aatattcaat cttgggcata tccggatttt gcctatacta aattgttcaa tgtgccatta
     4561 tttactggtt ttatgtatgc agcagttggt tcttacatta tccaagcgtg gcggttgttt
     4621 gatttgaaaa ttaaaagcca cctgccctat tggttagcca ctttaacggc actgatgatt
     4681 tacctgaatt tctttacgca tcattacatt ggggattacc gttggtatat tgccgctttt
     4741 gcattagggc tgtatgcacg tacttttgta tttttcaccc cttatgacat tacacgaaaa
     4801 atgccactgc tgctttcttt cttcctgata ggttttttca tttggttggc ggaaaatatc
     4861 ggtacgtttg ccggaatttg gcgctacccg aatcagtttg gggcttggtc tatggtgcat
     4921 atgagcaagt ggagtgcttg ggcgttattg gtgatgatga cttttaccat cattgtgcat
     4981 ttaaagcata tcaagcaaac agtcagagtc tcgagcgatt aacaagctgt gggttttggc
     5041 gaaaatttta ctcatcagtt attttcagtg gaattagggg caaataataa tttgccccta
     5101 tatcaaatta tggattttcc ccttccacat caataatcat cacttccgac tgtgagccta
     5161 accgcatcgg cacgccccag aatccgtagc cggaggtgac aaaataatgc ccgaagccga
     5221 ttttttcata gccgtaatgt aatcgataga tcagtttggt aataatactt gcagggaaca
     5281 cttgcccttt gtgagcgtgt cctgaaactt gaatatcaat cggcaattta gcatgttgct
     5341 caatttcagt cgggcggtgg tcaagcaaaa ttaccggtag tttagtgttc acgttttgta
     5401 aaagctgttc ggcagaaggt cgatttttga ctaaatcatc attacgccca ataagggtaa
     5461 attttccgtt aatttcggcc gtttgatccc ataaaacagt aataccggcg tcggtaattt
     5521 cttttgcaat cgtttcttga gccccaaaaa agtcgtgatt gccaagggta gcatacacgc
     5581 caagaggagc ttttaatttg gctaaatgag gctgcatttt atcggcaaca taaacttcgg
     5641 tattgtcgtc cataatatcg cccggcaata aaatcagatc gactttttgc tggttgaaaa
     5701 tctctgccag ttggtcaagt tgcttggttc cgaacagttt tcctaaatgc agatcgcttg
     5761 ccataccaat gcgaagcggt ttctctaatg gtttatccaa tttcaccgaa taatgtacta
     5821 ctgttggggt ataagcgttg taatagccaa gcccaagtaa gccaaagaat gcaaatgggt
     5881 aggcgatttt cagccagcga ttgacggtag tattgccgtt gaatattttg taaatacaag
     5941 cggtgaaaaa actgataaaa aatgcaaaaa gtaacaatac caatatgaat gccattattc
     6001 taaatagcgc aaaagtacga gtaagatgca aaatcactaa tccgtttgcg actaagaaag
     6061 tgagaatacc aactgtttta cggtttttac ttgataaatt aaataaccag ctgacagtct
     6121 ttgcaaagtt gaataaaaag agttgtaaca ccgcaatcac aagggcggtg gtaatgtagt
     6181 aacgatattc catatttttc cttcaataaa ttggctaaac ccagccgatt tgtcttaata
     6241 aaaataagcc gatgctggca ctaaacattg agccgagagt cgtaatgccg atcagatttg
     6301 cggctgcctc gccgtctccg ccatagttgc gtgtcattgc gtaggtggct gaagcaacgg
     6361 gagcagaagc agccacaaag acaatgccta gttccatcgg cggaagttga aacacaaatt
     6421 tcccgagtag tagcaagaaa attggagcaa aaatcagccg aatcgaggca gaaaacagca
     6481 caattttatt gatggtgctt tccactcctt gattaaattg tttcaactgt ttgaaattaa
     6541 tgcttgctcc ggtgcagata agtgctaatg gtagtgtcag gctcgccatt aatttaccag
     6601 cagactcaag cggggttgga atttggtggc ttaattcaaa atagttaaaa gttaagcctg
     6661 caatgacggc tagaatcagc ggatttctca atatcgacaa tattaatctg ccaaaattca
     6721 cttttttctc gcttaacgat gaagtgaggg taatcactgc aagcacatta aaggtaatca
     6781 ccaagcacga aatataaact gaagcggcag cgagagcttt atcgccataa gcattgagaa
     6841 ttaaggcaat gcctaaaatc gccgcattgg tacggaaagt gccttgagtg aaaatacaac
     6901 gataacggcg gttttcaata tgtcgagccg cccaccattc cgcccataaa tagatcaaaa
     6961 tcgaaccgcc gactccggtc gagatcaggt tgatttggct ggagaaatct aacgggcttt
     7021 ttagcatatt caagaaaatc atcggtggca gggtaaaatt aaataccacc tttgatgcca
     7081 tattgcaaaa ggtatcgtca ataaattttc gtctgcgtgg aaaaataccg aacagcatta
     7141 acaaaatggt tggtaaggta acgccaatgc taaattggat agagtcgaaa agcataatgt
     7201 ttgccttaaa tgatggcacg agtgtctttt gcttgtaccg tagattatca ataaagtacc
     7261 agcgtggacg ctggtactat catcaatgga aatgaaatta caatcctgcc tttaagcttg
     7321 cttcgacaaa tttatccaaa tcaccgtcca gcacttgttg ggtggtgcga tgttccacac
     7381 cggtacggtg gtctttaatc cgtgaatcgt ctagcacata agagcggatc tggctgcccc
     7441 agccgatgtc ggatttgttg tcttccatcg cctgtttttc cgcatttttc ttgctcattt
     7501 ccagctcata tagcttcgct ttaagctgtt tcatcgcctg atctttattt tgatgttgtg
     7561 aacgaccgtt ctggcactgc accacaatgc cgctcggtat atgggtaatt cgcaccgccg
     7621 attcggtttt attgacgtgc tgaccacccg cccctgaggc acgatacaca tcaatccgca
     7681 aatccgcagg gttgatttca atatcaatat cgtcatcgac ttccggataa acgaaagccg
     7741 ccgagaacga ggtatgacga ccgttattgg catcaaacgg gcttttacgc accaagcggt
     7801 gaatacccgt ttcggttctg aaccagccgt aagcatagtc gccggaaatt ttcacggtgg
     7861 cggatttaat ccctgccaca tcaccgtctg aggcttccat tagttcggct ttaaaccctt
     7921 tgctctctgc ccaacgcaaa tacatacgca gtaacatttc tgtccagtcc tgagcttccg
     7981 ttccacccga accggcttgc aaatccaaat agcaatcggc actgtcgtgt gggccgctga
     8041 acatccgctt aaattctaaa tcggcaagtt ttttctctaa ttcgtctgct tccacttggg
     8101 cttcattgaa ggtatcctga tcttctgctt ccaccgccag ctcaatcaac ccttcaacat
     8161 cttcaatccc ttgctctaag gctttaatcg tattgacgat ctgctccaac attgcccgct
     8221 ctttaccaag cgcttgtgct ttttccggct tgttccatac atcgggttgt tccagctcgg
     8281 cattgacctc ttctaagcgt tcgactttat gctcaaagtc aaagataccc cctgatcacc
     8341 gcggtgcgct cgcttaaatc agttagttgg tttttaattg ggtttaattc aaacattgtc
     8401 gttcctgttt ttgcttattt taaaaagatg aattatagct gaagttaagc aagcggtcga
     8461 attttgcaag aaatggagca atgatcgttt ctagtaatga gatttggttc ataaaagttg
     8521 caactgtttc tgctatttgg ctttgatagc taaaaatctt taaaaaaaca agcggtcgtt
     8581 ttaccccatt ttttgcaaaa cgaccgtagc ttcttgctat aatcgctgaa aaaatgctgc
     8641 aaggagattt tatggcaaag cgcccaagtg atattgatgc aaccctattg caactggaaa
     8701 tcctacgccg tatccccaaa ttacccgcta aaattgatgc taaaagtttg caccaacagc
     8761 tattagatgc cggctttgaa cgggatattc gcactgttca gcgttcgctc aaaatgttat
     8821 gcgagcattt tgatattgaa tgcgatgagc ggagcaaacc ctttggttat agctggaaaa
     8881 tccgctcaga ggggctggca ttgccgattc tcaacgaaca acaatcatta ctgctaaaac
     8941 ttgccgagca acagctgaaa tatctgttac ccgcaaattt aatggcatca atgaaaccct
     9001 tttttgagca ggcagacaga atggtttccg gggcgaaaga caagcccgaa taccaatggc
     9061 taaataaagt ttgctccacg ccaaccagcc taccgctgat ccctgcaaaa attaaagaag
     9121 agatttttaa ttccgtaagc caagccttat ttcaaaataa gttgttaaag atcgaatatc
     9181 agaaccagca cggcaaaaaa caccaagcga caattatgcc gttggcgatt tcacaacaag
     9241 gtgcgagcat ttatttggtc tgccgttacg aagggtttga cgacaaccgc ctgttggctc
     9301 ttcaccgcat tcgtaaagcg gagatttcta cttttagttt tgaacgcccg aaagatttta
     9361 atttgcaagc ctaccaagat gccggacacc tcggttttgg caatggcgaa aaagctagcc
     9421 ttaccttttc gattagcaga gacgccggtt tccatttaac cgaaacaccg cttgccaaag
     9481 atcaaaaaat tctcgaggag agtgaaactc actaccgtat tcaagccata gtcgctgata
     9541 atgatatgct aacctggtag ctacgcaaat ttggcgaaga tatttgggat atcgagaaag
     9601 aagcgctaaa ataagctaaa caagcggtcg gttttgtggg aatttttgct aaatagtatc
     9661 gaccgctcct ccctaaacag acctttatca taaatacaac tctttcaatc agatattctt
     9721 actgtctatt gatgaacgat tcgaattttt ctagtggttg aaatttaaac taattttctt
     9781 gctttttggc atttttcaga cttttaattc tctccattag cctgtttttt tacgctcttt
     9841 ttcataaaca ggaataacca atgccttttc aacttacttt ctgccaacaa gccggcaatc
     9901 agcaccagaa aaatcaagat gcactgttta acggcaaagc ggtgtaccaa tggcaactta
     9961 aaaatgccga gagttatctg ctggataacg acaaagtaat ttttggggtt gcggacggcg
    10021 tttccagcca ccctcgaaat catcttgcct cacattattt aatggcgtta ctcagccaat
    10081 gccaatccct tgatagccaa tggttgcgac aggctcacgg cgatttttgc gaaaaattag
    10141 cgaaagatta ttttggtgct tcttgcactt ttgttgcttg tgaattacac cataacggca
    10201 gagggcgtat ggttaatgtg ggcgacagtc gagcctataa aattacggca aacggggagt
    10261 ggcaacaact gagttttgat cacaccatac tggctgaaat gaaacagaaa ggcttggtta
    10321 aggaagggag caactatgcc tcaatgtata acggcttaac cgattgtatt gttgccgatt
    10381 ttgacgccga tgatttccgc attcatatag ccgaatttca actggaaaaa ggcgagagca
    10441 ttttgctctg ttctgacggt ttgcacgaac aactcgcccc agaagctatt caggcgattt
    10501 aggacggata cgaaagcccg tctcatcgtc tgcaaatttg cagaaaaatg gtaaaaatgc
    10561 accgcttgta tgacgatttt tctgcggtga tttgtgaaat tgtgtaatta aaacgacatt
    10621 atttgtcgca ttggtgctta aaatccgcat aaaacagagg agaaaaaaat gaaaaacgat
    10681 ttccattatg ccgctgaatt aatccgcaat gcagacggca ttttaattac cgcaggggct
    10741 ggaatgtcgg tggacagtgg cttgcccgat ttccgtagcg tgggagggtt atggaattcc
    10801 tacccaccgc tcaaaaaatt taatttgcaa tttatgcaga ttgccacacc gcttgcctat
    10861 caggaacgcc ctga
//
LOCUS       DANLUA010000081        10814 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLUA010000081 DANLUA010000000
VERSION     DANLUA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            35..367
                     /locus_tag="PXB44_001476"
     CDS             35..367
                     /locus_tag="PXB44_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5281950.1"
                     /translation="MALSVSSILSSQATYAEETTAELSEVNVVAKEDVKASIEKKNKE
                     IIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNRVGISIDGVSLFLIQKKI
                     PFINAMVI"
     gene            334..2412
                     /locus_tag="PXB44_001477"
                     /pseudo
     CDS             334..2412
                     /locus_tag="PXB44_001477"
                     /inference="COORDINATES: protein motif:HMM:TIGR01786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            2467..2973
                     /gene="hutX"
                     /locus_tag="PXB44_001478"
     CDS             2467..2973
                     /gene="hutX"
                     /locus_tag="PXB44_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL5281951.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(3053..4270)
                     /locus_tag="PXB44_001479"
     CDS             complement(3053..4270)
                     /locus_tag="PXB44_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL5281952.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            4431..7964
                     /gene="mfd"
                     /locus_tag="PXB44_001480"
     CDS             4431..7964
                     /gene="mfd"
                     /locus_tag="PXB44_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL5281953.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            8127..8414
                     /locus_tag="PXB44_001481"
     CDS             8127..8414
                     /locus_tag="PXB44_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5281954.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            8415..8627
                     /locus_tag="PXB44_001482"
     CDS             8415..8627
                     /locus_tag="PXB44_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5281955.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            8743..9003
                     /locus_tag="PXB44_001483"
     CDS             8743..9003
                     /locus_tag="PXB44_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5281956.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            9004..9393
                     /locus_tag="PXB44_001484"
     CDS             9004..9393
                     /locus_tag="PXB44_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5281957.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            9413..10651
                     /gene="pepT"
                     /locus_tag="PXB44_001485"
     CDS             9413..10651
                     /gene="pepT"
                     /locus_tag="PXB44_001485"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL5281958.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
BASE COUNT         3247 a         2158 c         2472 g         2937 t
ORIGIN      
        1 ttcaataaag tctaaaaaat tgtgcccgac tatattggct ttaagcgtta gtagcatttt
       61 gtcttcacaa gctacctatg cggaagaaac aacagcggaa ttatctgaag taaatgttgt
      121 tgctaaagaa gatgtgaagg cttctataga aaagaaaaat aaagagataa ttcagcaaga
      181 gttgattcaa aataatcgag atttagttcg ttatagccct gatgttggca ttgttaacca
      241 aggtagacat caaaaaggat ttgctattcg tggagtggaa gacaaccgtg taggaataag
      301 tattgatggt gtctcgcttt tcctgattca gaagaaaatt ccctttataa acgctatggt
      361 aatttaaata catctcgcca atctatagat ccggaattag ctcgtactat tgaagtatct
      421 aaaggtgcag attctttcaa tcaaggtagt ggtaatttag gcggcggagt taattatcgg
      481 acgcttgagc cttatgatat tgttcgtaac ggtaataaat ttggtgcttt ttatcgcaca
      541 ggatatggta ctcgtaataa tgaatggttg aatacattcg gtgttggata tttaggtgag
      601 aaagctgagg cgatattggt ttattcaaat cgccatgggc atgaaatgaa aagtgcagga
      661 ggttatacac ttcctgaaga ttctctacaa actcgctctt tacgcagtag taaacaaact
      721 cctgatgatt ctactcataa tcatcataat tatttagcca aatttgctta tcgatttaat
      781 gataatcacc gcgttggtgt ttcttacagt gggcaaaata ataaaaatta tattatcgaa
      841 gattcagccg ttactattac ttctttttgg cgtgaagcgg aagaccgtag taaacgtgat
      901 acagtaaatg ttttttatga gtatttccca gagtcaaaat ggatatctct agcgaagata
      961 gatgcagatt atcaagatac tgaaacatct gcttataact atgaaggtac acgcgctgaa
     1021 gatgcaacag actggagccc tgctaaacaa agaaaaccaa gtgacaataa tattcgtatt
     1081 tttaatactg aattgaaacg cttaaatttc cgtttagaca gccaagcatt agaatttggt
     1141 aaatggactc atcagctatc tttcaaagcc tctactgcag agagagattt cgatgtatta
     1201 cataaggact cggtttattt atccggtgtt tgggtaaatt ctcccgattc tacgatgatg
     1261 tacccaatta aaacgaaaca gcacactttt tcattgcacg ataagattga tttaacgcat
     1321 gattggattg caacgctagg cattcgttat gattgggcga agtatgaaca acaagaactc
     1381 aatggtcttg tttgtcgtaa ctgcgtaaaa actgacaatg cgaaatttaa tcaactaact
     1441 tgggttaccg gtttagaaaa acatctgact gaggcatgga aagtaggata taacattggg
     1501 acaggcttcc gtattccaaa tgcatcagaa atgtattttg attaccgtga taatgctgca
     1561 ggagcttgga tgtccaatcc aaacctaaaa gcggaacgta gtttaagcca aaatttaagt
     1621 ttgcaaggta gtggcaatgt tgggctgctc tcagttaatt tccaccatac taaatataaa
     1681 gattttttat gggagcaaga aacctgggat gtttaccaag catatggtag agagttttgg
     1741 cgcccggtac agcaaatgca aaatattgat tctgcgaaaa tctacgggct agaagtgagt
     1801 ggtaaatgga atttaaattc agctatgcca atacctgaag gctggaagct atttggagcc
     1861 ttcggttaca gcaaaggtag tatgtctaac ggggcagatt tactttctat tcagccgatt
     1921 aaagccgtag ttggcttaga ttatgaacaa cctgaaggta aatggggagt attttctcgc
     1981 ttcacattct tgggggctaa aaaggcgaaa gatgcaaaat atttaaaaac attaccggaa
     2041 aaatgcctta aagaggatag aacgccaaat ccgtattacc cttattgggg ggatgaatat
     2101 gacattcgtt gtactgaata ttctcacgag acaggtttag atacctggaa gcacttaaat
     2161 gctaaagcct ttgttgtgga tttatacggt ttctataaac caactgagaa tattacatta
     2221 cgtgccggta tttataattt gtttaaccgt aaataccata cttgggatac tttacgtggt
     2281 ttaaatacaa caggtggaat cgttaattct gttggagtac gtgaaaatgc aacttacggt
     2341 ggctatcctg gattacaacg ctattatgaa cctggacgta acttctcggc taattttgaa
     2401 taccgtttct aatttacaaa tttacttaga gagctaagaa actctcttta cttaaaggaa
     2461 catttaatga tatcaatcga acaacaaacc gccttacgca atgaattagc cgaaaatccg
     2521 gggcaaattt tagaaatgtt agcggcaaaa tatcaatgca cgctggaaga ggtaatgctg
     2581 aatttaccgg ctgaaatgct aaaagtaacc gaaggggaac gttttgccga agtgctacag
     2641 caaatccacg aatgggacga tgcgattacg ctgatttcgc atacggaaga taccattatt
     2701 gagtttgtgg gaaagctccc aagcggttca atttctcgtg gattttacaa tttcgaacat
     2761 aaagagggcg gaggcttaca agggcatcta cgctacgaaa attgtgccaa aatttatttg
     2821 ttggatcgtc cttttatggg aaaacgtact gtttcactga ttttcattaa taaaaaaggc
     2881 aacgcaatgt ttaagatttt tgtcggcaga gagaaaattg gcggggcatt aaaagagcat
     2941 caaattaagg cgctgtatca gttgattggc taatctgtaa aaagtcatcg aaaaatgacc
     3001 gcttgtaact tatagaaagg gctacgatat tgaatcgtag cccttttcaa tattagccta
     3061 ccgcttgcgg taaatagcca gcctgaatta aaaacggaat gaccataatg ataataccgg
     3121 caattaacgc aaccactaat gccaaattac caccaatcac acggtaaggt aaattcggat
     3181 attggcgacg tgctttccac gctaagccga taggtaaaat cataccatag aacgcaaaga
     3241 gtaaaccggc gtaacctaat gccttaataa agccgttcgg atagaataaa gcgaacagaa
     3301 ttggcggtgt aaaggtggcg atggtcagcc ataaacggtt agtcggtaga tttaagcgtt
     3361 ttaacaaatc gcccacgcct tcaaaaattc ccatcgaaac ccctaaaaat gaggtaatca
     3421 atgcaagcac tgagaataag cgaaccattt cgcctaaaat cgtactaccg gtaatctggc
     3481 tagtggcttt caccaagccg tttaaggtcg gatcttgtgt caaaattgtg gtgaattcat
     3541 tttggcttaa taccccgtgt gtagcgagct gccacaataa ataagccact agcggaattg
     3601 ccgtcccgat ataaatagat ttacgaattt tgccgatatc tgcgtctaaa tagctattaa
     3661 ttgttcccat aataacgtgg aaaccgaacg cagtgaagaa aatgggtata gcagaaataa
     3721 tgagtaagtt atctaatgga attgctgtca agttttccag cttcgcttta ggcagcatca
     3781 tcagcaacac ggcaataaaa gcaacaattt ttcccaaaaa cagtactcgg gttaaaccat
     3841 caaccgattt cacgccgact accacaaatg tacctaatac cgcagtgaag attaaaatgc
     3901 tgattttcag tttcatatca gcatctgcca tttcaggcat tacaccggct aacagtgaac
     3961 caccaccggt aatataagcc gcaagtaggg cataaagtag tacgagcaaa ctgaaagtgg
     4021 ctaatacacg cccgattaag ccaaagtatt gctcggcaag gcttgccact ccaacatctt
     4081 tttgcggagc ggtttgataa acttccataa ataataaacc ggtgtaggcc aataaagccc
     4141 ataatccgac taagagtaat gcggttgccc caaaacccat tcctgcagag gtgagtggca
     4201 ttgcaagcat tcctgcacca atggtagtgc ctgcaatcat taatgcactt cctaaaattt
     4261 tatttttcat aatatgtgtg agtaagagtt aaaaaagggg aacattttat cagaattttt
     4321 tcgcaatgcg atctagataa cataatttct ccgcttattt tgcacaatca gccgtgcttt
     4381 agtaaactaa gcaacaattt gcaatttttt atttaaaaca gaccgcttgt atgtctttaa
     4441 tttcacttaa tctgccgaaa tctgacggca attttcaaga tcatcaaacc ctcggtaacc
     4501 ttgtcgggca ttccgatacc cttgcgatca gccaagctgc ccgagccttt tacggcttga
     4561 cggtggttgt cacacccgac acccgcacgg cgttacgctt ggaaaaagcc ttgcctcagt
     4621 ttgcagggct gccagtgcaa ctgttccccg attgggaaac cttgccttat gacaattttt
     4681 cgccgcacca agacattatc tccgcccgac tttctgccct gtttgaactg caacaagggc
     4741 gacagcagat tttgctgttg ccgatcaaca ccttaatgca gaaggtctgc ccgccgaatt
     4801 atctcgctaa caatgtgctg ttgattaaaa aaggcgaccg tttttcgatt caaaaactgc
     4861 ggttacaact ggaaaatgcc ggctatcggg cggtggaaca ggtgttggaa tatggtgaat
     4921 acgccgtgcg tggctcgatt ttagacctat tcccgatggg agcagaagag ccgtttcgcc
     4981 tcgatttttt cgatgatgag atcgacagta tccgcacttt tgatgtggat aaccaacgca
     5041 ccaaaacgga aattgccgaa attaatttac tgccggcaca cgagttcccg actgatagca
     5101 acgggatcga gcattttaga gccaaattcc gtgaaaaatt cggtgaaatc cgccgtgagc
     5161 cggagcatat ttatcagcaa gtcagcaaag gcattttaaa tgcagggatt gagtattggc
     5221 agccgttgtt ttttgaggaa atggcaagtc tgtttgacta tttccctgcc caaacgctgt
     5281 ttatcacttt cgccgatatt cagcaaaaag ctgaacaatt tcaccaagat acccaaaacc
     5341 gctatgaaag ccaccgagtg gatccgatgc gaccgctact gccgccgagt gatctctggt
     5401 ttgcgattga tgaggtaaat cgctggctga aaggctatcc ccgtttaacg ataacttcgg
     5461 agaaaatccg taaatcagca gcgaaaatga atgcaaatgt ggctaacttg ccggatattg
     5521 ctattcaatc ctctgccaaa gagccgtttt ctgctttcca gcaattccgc caaaaatttg
     5581 acggcaacat tctgttttcg gtggagagcg aagggcgaag agaaaccttg ctggatttgc
     5641 ttgctccgct tggggtgaag ccgaagcagg tcaattcatt agatgaagca caaggtttgc
     5701 cattttccct gattatttca ccgcttgatc aagggtttgt ggtggaaacg ggcgggcacg
     5761 ggacccgtcc ctacgatgat gaattaaata caaatggcga aaatgataat aactacgtag
     5821 gggcgggtcc tgtgcccgcc cgcaaccttg ccatcatctg cgaaaccgac ctgctgggcg
     5881 aacgtgtgca aacacggcgt gagaaaaacc gcaaagccgt caatcctgat accttaatcc
     5941 gtaatttagc agaactcaaa atcggacagg cggtggtgca tttggaaaac ggcgtggggc
     6001 gttacggtgg cttaaccgtg ctggacgctg gtgggatcaa agccgaatat ctcgtgctac
     6061 attatgctaa cgatgccaaa ctctatgtgc cggttgcctc gctccattta atcagccgtt
     6121 atatcggtgg ggcggatgaa actgcgccgc tacataaatt aggcagtgaa gcgtgggcga
     6181 aaacccgcca aaaagcagcg gaaaaaatcc gtgatgtggc ggcagaattg cttgatgttt
     6241 atgccaaacg agaaagccaa accggttttg cgtttgccta cgataaagcg gaatttgatc
     6301 agtttagtgc cactttccct tatgaggaaa cggacgatca gaaaattgcc atcaatgccg
     6361 taattagcga tatgtgccaa gccaaagcga tggatagatt ggtgtgtggc gatgtcggct
     6421 tcggcaaaac cgaagtggcc attcgagcga ctttcttggc agtgatgaac cataaacagg
     6481 tggttattct tgccccaact accttgctcg cccaacagca ttatgagaat ttcaaagacc
     6541 gtttcgccaa ttaccctgtg aatgtggaag tgctgtctcg ctttaaaacc gccaaagagc
     6601 agaaaacgat tttgcaaaat gtggcggagg gcaaagtgga tattttggtc ggcacgcaca
     6661 aactgttgca agacgatgta gcgttccgtg atcttgggct tttagtgatt gatgaagaac
     6721 accgtttcgg cgtacgccaa aaagagaaaa tcaaacaact gcgtgccaat gtggatattc
     6781 tgaccctcac cgccacgcca attccaagaa cgctgaatat ggcactcaac ggaatgcgtg
     6841 atctctcgat tatcgccagc ccgccggctc gccgactttt aatcaaaacc tttgtgcgac
     6901 aaagtgatga aatggtaatc cgtgaggcaa ttttgcgtga aattctgcgt ggtgggcagg
     6961 tttactacct ccataacgat gtggcaacca ttgaaaattg tgccgagaaa ttggctgaac
     7021 tcgtgccaga ggcaagaatt gtgatcggac acgggcagat gcgtgagcgt gaattggagc
     7081 gggtgatgag cgatttctat catcaacgct ttaacctgct ggtttgctcc accattattg
     7141 aaacggggat tgacgtgccg actgccaata ccattattat cgaacgggcg gacaaattcg
     7201 ggctggcaca actgcaccaa ctgcgtggac gggtcggacg ctcgcaccac caagcctacg
     7261 cctatatgct cacaccgcac ccgaaaacgc tcaccaaaga tgccgagcaa cgcttggagg
     7321 cgatgagtac cattgataat ctcggggccg gttttgcgtt ggcgacccac gatttagaaa
     7381 ttcgtggtgc aggcgaattg ctcggttcgg agcagagcgg acagattgaa agcatcggtt
     7441 tttcgctcta tatggacttg ctcgaaaatg cggtcaaagc tttgcaagaa gggcgagaac
     7501 cgagcctcga tgaaatcacc caacaccaag cggagattga attgcgtgta ccggccttat
     7561 tgccggacga ttatctgcct gacgtgaata tgcgtctgtc gttctacaag cggatcgcaa
     7621 gctcggaaaa tctgcaaagc ctgaacgact tgaaagtgga gctgatcgat cgcttcggct
     7681 tgctcccaga ggcaaccaaa aacctgtttg cgataaccca acttcgcttg tcggcacaaa
     7741 cgctcgggat taagaaaatc gatgccggca ttcacggcgg ttatcttgaa tttaaaccga
     7801 cagcccagcc cgacccaatg aagtttatcg ccttaatcca aaaacaacca gcggtgtaca
     7861 gttttgaagg ggcggtgaag ttcaaattcc gcttagcact cgaagacaac cgcaagcggt
     7921 tagaatttgt ggaaaatttg ctaagggagg ttgtgggcgg ataaagcctc ctaaggttgt
     7981 caatcttgaa aaacgatttt tgcgatctgt atcgtaaaaa tcgtttttgt ttattgcgaa
     8041 tccagtaatt tcatctaggc tgtttataac ttaaaaaaat agatgtaaat atattttatt
     8101 tacaattaaa aaggagtaaa ctggcaatgg aagaaattaa tggttttgaa tgggatagtg
     8161 agaaagctct tcgtaatcaa caaaaacacg gagttagttt tatcgaggcg gtaagcgtat
     8221 tttatgataa taatgcgatg ttaatcccgg atccagatca ctcatctagg gaagagcgtt
     8281 ttttgctact cgggcgtagc aataaagata atctacttgt ggttgtgcat tgcgagagaa
     8341 ataccaacat tcggattatt tcagcacgtt tggcaacaaa acgcgaaagt gggcaatata
     8401 aaggagtaca ttaaatgaga gatgaatacg atttttctaa tgcagtgaaa aacccttacg
     8461 caaaagggct aaaaagcaaa agtgtggtta ctattcggat tgaagacgat gttatcagct
     8521 attttaaaca acttgccgaa gaggtgaatt tgccttacca aacgttgatc aatctttatt
     8581 tgaaagattg cgttgagcag aaacgtaaac cgactattag ttggtaatat tatgatttga
     8641 aatcagtatg ttattgagta tttgagaatg ggttatcttg ccagttgtat aaaataggca
     8701 tatactttgg catatacact atctatcgga gggtaaagta agatggaaaa aattgcgaga
     8761 ttattccgta acgggcggaa ccaagcagtg cgtttaccgg ttgaatttga gtttgatgca
     8821 gagcaagtct atgtttctaa aaaagaaaat ggcgatattt tattatcatt gagatcgaga
     8881 aataccgagc attggcaacg attgtttgag ctattgcctg ctgttgtatg tgatgaaaca
     8941 tttttagata caaaagagag aaaccaatcc tttgagcaac gggatccttt tgaggtagct
     9001 taaatgtata tgttagatac gaatactgta agctattttt ttcgtggaaa tgcaaatgtg
     9061 gtggcgaaat tgaagtccat taatcctgag aggctttgta tttcatctgt aactgctgct
     9121 gaattggttt atggtgtggc gaaaagaaat aatgggcagc tttctcaatt tttagattat
     9181 tttttatcga gtgtaaaggt tttagattgg aattatcgtt gtgctgaact ttatggaaag
     9241 ttacgggcag agatggagaa aaatggcaaa gtaatgggcg tgcaggatca aatgattgct
     9301 agtcacgctc ttgctgaaga gtgtatttta gtttctagtg atcaggcatt taaaatggta
     9361 cctgatctga aattggagaa ttggttacac taaactatag taaggaaaaa caatgaaaca
     9421 atcccttctt aaccgtttac tacgttacac ccaaattcac acaacttcag atccgaaaag
     9481 ccaaactgtg ccaagctcgc aacagcagtg ggatttgctt catctgctga aaacggaagt
     9541 agaggcgatg gggctaagcg agatttatct tgaccccaac ggctatctgt tcgccagctt
     9601 gcctgccaat attgacaaaa ccctgccggt gcttgggctg attgcccacg ttgatacttc
     9661 gcccgatttt aatggtgaaa acgtgaaacc acaggtgatt gaggcttatc aaggcggcaa
     9721 tattttgcta aacggcagtg gcgattatct ttcgccaacg gatttcccga atttaaacaa
     9781 gctgattggc gacaccttag tcaccactga cggcacctct ttgcttggcg cagacgacaa
     9841 atccggcatt gcgattattc tctcggctat cgagtattta caagccaatc cgcatattcc
     9901 gcacggcaag atccgcatcg gctttaccac cgatgaagag atcggtcgag gcgcagatca
     9961 ttttgatgta aacgcattcg gtgccgatgt cgcctttacc tttgatggcg ggcctctggg
    10021 cgaattgcaa tatgaaaatt tcaatgccga tgcagcgacc gttacgttct tcgggcgtag
    10081 cgttcacccc ggcacggcaa agggcaagat gatcaacgcc ttagagcgag cttgcgagtt
    10141 ccaacaccgt ctgcctcccc atttaacccc tgccaccagc gaagggcgtg aaggctttat
    10201 tatgttgcac ggcattgagg gtggaattga taaagcggaa attcactata ttttacgtag
    10261 ctttgagcgt gatgaattag ctgcattcgg caaaatgctg aatgatacgc tggcagagat
    10321 gcaacagatt tacggcgaac attgcggaaa agtcgagatt gtcgagcaat atcgcaatat
    10381 gcgtgaagtg ctggacgatt acggctactt ggttgaaatt gccgaaaacg caatgcgtga
    10441 acaaggcatt gagccgaatt tagaaccgat ccgtggcggt actgatggct cacgcctttc
    10501 atttatgggc ttgccttgcc cgaatttatt taccggcggc gaaaatttcc acggacgatt
    10561 tgagtttatt tcggttgata cgatggaaaa agccacaaaa gttgtaattg atatttgtcg
    10621 gaaatttgct gattatgatt tcaacaaata aggcggctgc aagcggtcaa atttgatgaa
    10681 aaatttgcaa aaagcgttat ttttcaccaa ttaaacgatt ttttacagat ttcctacaaa
    10741 tttatttgac attaaatctg aaatccatag aatatgcacc tgttgagact ctacgaggtt
    10801 cgtagcgttt ttaa
//
LOCUS       DANLUA010000082        10474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLUA010000082 DANLUA010000000
VERSION     DANLUA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            28..1536
                     /locus_tag="PXB44_001486"
     CDS             28..1536
                     /locus_tag="PXB44_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL5281959.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(1601..1822)
                     /locus_tag="PXB44_001487"
     CDS             complement(1601..1822)
                     /locus_tag="PXB44_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5281960.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(1851..2360)
                     /locus_tag="PXB44_001488"
     CDS             complement(1851..2360)
                     /locus_tag="PXB44_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL5281961.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            complement(2467..3801)
                     /locus_tag="PXB44_001489"
     CDS             complement(2467..3801)
                     /locus_tag="PXB44_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL5281962.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            complement(3912..5060)
                     /gene="dapE"
                     /locus_tag="PXB44_001490"
     CDS             complement(3912..5060)
                     /gene="dapE"
                     /locus_tag="PXB44_001490"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL5281963.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            complement(5190..5537)
                     /locus_tag="PXB44_001491"
     CDS             complement(5190..5537)
                     /locus_tag="PXB44_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL5281964.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(5537..6395)
                     /gene="folP"
                     /locus_tag="PXB44_001492"
                     /pseudo
     CDS             complement(5537..6395)
                     /gene="folP"
                     /locus_tag="PXB44_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
     gene            complement(6504..7466)
                     /gene="rluC"
                     /locus_tag="PXB44_001493"
     CDS             complement(6504..7466)
                     /gene="rluC"
                     /locus_tag="PXB44_001493"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL5281965.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            7575..8477
                     /locus_tag="PXB44_001494"
     CDS             7575..8477
                     /locus_tag="PXB44_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL5281966.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            8575..9072
                     /gene="rraA"
                     /locus_tag="PXB44_001495"
     CDS             8575..9072
                     /gene="rraA"
                     /locus_tag="PXB44_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL5281967.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(9120..10463)
                     /locus_tag="PXB44_001496"
     CDS             complement(9120..10463)
                     /locus_tag="PXB44_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL5281968.1"
                     /translation="MKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQKNVIAE
                     SAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAFGVSGGS
                     ILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGDLLQERL
                     NFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDLEISRAV
                     AASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHILSLYQDS
                     KERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVNAQNEVS
                     SEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSKIEMHFV
                     SLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALKTLQ"
BASE COUNT         3060 a         2349 c         2044 g         3021 t
ORIGIN      
        1 ttatttctaa tttatgagga ttctattatg tctaaatgtc ctgttacaca tttgacaatg
       61 aacaacggtg cacctgttgt tgataaccaa aacagcttaa cagcgggtcc acgtggtcca
      121 ttattagcgc aagatttatg gttaaatgaa aaattagcga acttcgtgcg tgaagtcatt
      181 cctgagcgtc gtatgcacgc taaaggttcc ggtgcgttcg gtactttcac tgtaactaac
      241 gacatcacta aatacactcg tgcggcaatt tttagccaag tcggtaaaaa aactgaaatg
      301 tttgctcgtt tcaccaccgt tgcaggtgaa cgtggtgcgg cagatgctga gcgtgatatt
      361 cgtggtttcg cattaaaatt ctataccgaa gaaggtaact gggatatggt gggtaacaac
      421 acccctgtgt tcttcttacg tgacccacgt aaattcccgg atttaaacaa agcggttaaa
      481 cgtgacccac gcacgaatat gcgttctgca actaacaact gggatttctg gacattatta
      541 ccagaagcat ttcaccaagt gactattgta atgagtgaac gtggtattcc ggcttcatat
      601 cgtcatatgc acggttatgg ctcacatact tatagcttca tcaatgcagc taatgagcgt
      661 ttctgggtga aattccattt ccatacacaa caaggcatta aaaacttaac taacgaagaa
      721 gcagccgcta ttatcgctaa cgatcgtgaa agtcaccaac gtgatttata cgaagcgatt
      781 gagcgtggcg atttcccgaa atggaaaatg tttgttcaaa ttatgcctga aactgatgca
      841 gacaaagtac cataccaccc gttcgattta actaaggtat ggccgaaatc agactatccg
      901 ttaattgaag tgggtgagtt tgaattaaat cgtaaccctg aaaacttctt tgctgatgtg
      961 gaacaatctg cattcgcacc aagtaactta gtgccgggca tcagcgtgtc tccagaccgt
     1021 atgttacaag ctcgtttatt caactatgcg gatgctcaac gctaccgttt aggtgtgaac
     1081 caccaccaaa tccctgtaaa cgctccacgt tgcccggttc atagcaacgc tcgtgacggt
     1141 caaggtcgtg tggacggtaa ctacggtagt accattcact acgaaccaaa cagcttcggc
     1201 aaatggcaag aacaagctca atatgcagag ccaccactga aaattaatgg tgatgcagcc
     1261 cactggaact atcgtgaaga cgatgctgac tacttcagcc aaccacgtgt attgttcaat
     1321 atgatgaatg atgaacaaaa acaagcatta ttcaataata ctgctgcagg tatgggcgat
     1381 gcattagact tcattaaata ccgtcatatt cgtaactgct atgcctgtga tccggcatat
     1441 ggtgaaggtg tggcaaaagc attaggtatg acggttgaag atgctattgc tgcaagagca
     1501 acagacccag cattaggtca accgggttta ctctaatcct tagtaaatac aaaactcaaa
     1561 gcaaaagctc tacaaaaaag tagagctttt ttattgctaa ttacttaacc accgtaattt
     1621 cccacaaggc atcaccaagt tgttggtaat cggtaacttc atagccttcc tcttccgccc
     1681 aatttggaat ggcttcggtt gcttgagtac agtcaaattc aatcgttaag ctatcgcctt
     1741 tattcaattt tgccatcgcc gctttggctt cgactaatgg aaacgggcaa acaagccctg
     1801 aggcaggtaa acgtacgttc atctttatta ctccttaaaa aattaacctt ttaaatgctg
     1861 tgggcggata atgccaatgt aattacactc aggataaaca ctaacaccca aggggaaata
     1921 ccaaatgttt cataaatcgt gcggtgttga attacattgc tttttagctc ttgattaagt
     1981 ggggctaaca caccggttcg catagaagca gaaaacagcc cataaataac cagtgcaacc
     2041 caactgccga ttaaaccctc accggcacga taccacgttc ctgtagcaca accgcctgca
     2101 taaataatgc cgataccaaa cagaaatgcc ccaatgatca ctgcgagcaa aggcaaatct
     2161 tccgccggag aactaaaaga gccggcttca tacaaaagcc aaatcccgat cgattgcacc
     2221 gtaattgcaa ttaaaagtgc cacaaacata cggctattct ttgttacata gagatcacga
     2281 aatgctccgg taatacaaaa acgccctctt tgcatcacaa acccgagtag aaagccacat
     2341 aataatcctg tgaataacat aaaaaatacc tctcaacaaa ttaacgtgag aggtatttaa
     2401 atataaagcc gtctagatgg ctaataatct ttagcaaaat gatatgttat tcagttataa
     2461 atgctattaa atatgtggaa ttacatcatc aggtgcacct aatttattga gtgccacttt
     2521 tcttggatct accaacttgg atttttttat catcggttca accgttgaac cttgtactaa
     2581 aatcgagaaa gtgactaccg cataagtcat taacaaaatc aaatcacgta catcaagatt
     2641 gccttctgag ctcgatacca ccgcagcccc tgccggtacg gaaagtgcca tcgcaagggc
     2701 taatccacca cgtaaaccgc cccaagtcat aatttttaag gtgtacgggt tataagtacg
     2761 gaaacgtttt aacacttggt atggaatcca caggctgata tagcgtgcaa gcaaacaaat
     2821 tggaattgct gccaacatta acaccccacc aataaaggtg aaatcaacta acagtgtact
     2881 taagccgata agcaggaata acaggtagtt taatgaatgg tcgatcatct cccagaagtg
     2941 atcaagatat ttttggctct gttcggagaa accggaacgg cgagtccagt tgccaatcat
     3001 aataccggca actaccattg ttaatgcccc tgaaacgtgt aggtagtttg caatcacaaa
     3061 gccgactgtt ggaatcgtta gcgtaattaa aatttcaagg cttccgtcat cggttgcgga
     3121 aataaattta tgtgccacaa aaccggttaa taagccaaaa acgataccgc ctaccgcttc
     3181 ttgcaagaac agtccggtaa taccgctaag gtttggttct tgtccgccaa aagcaacggc
     3241 aaagatagtg gtaaaaatca ccaagccgat accgtcatta aacagagatt cgccctccac
     3301 gtgcatcgaa aggcgttttg gggctttcag gcttttgata attgccaata cggcaatcgg
     3361 gtcggttggc gaaatcagtg agccaaacag caaacaatac acaaagccga gctctaaccc
     3421 aagtagcctt gccacgccat aaattaacgc accgatcaag aaagtagaaa ctaaggttga
     3481 aaataaggca taaactgcaa tttcccgccg ttgctctttc attaatggta gtttaatccc
     3541 aagtgaaccg gcaaaaagca agaaccccag cattccgttt aacaggaagc ttttaaaatc
     3601 gagttgttca aaaatggaaa tggcaagttt atcaacatta aaccagccga gagaaccgaa
     3661 ggtaagaata agaagggagc cgacaagggc agaggctgta attgcaatag tggtttgaat
     3721 attttcgttt aattttcggg taaaaaacga aataaacatc gcaagcccaa acagcaagca
     3781 gatataagag tagagttgca taacatttac ctataaatat aaaaactgcc gctagtgtaa
     3841 acgctgttta acagaatagc tacataaaaa ataggcagaa tacaatttga taacattctg
     3901 cctatttagg gttatgcagt tgccaataat ttttctaaaa tttgataata aatttcgcca
     3961 caatgtgcca aatcatccgc actcacacac tcattcactt tgtgaatagt ggcgttcagt
     4021 ggtccaaatt ccactacctc tgcccccatt aatgcaataa aacgaccgtc cgaagtaccg
     4081 ccacctgtgt ctaaacgagg cgtaattttt gtgacatttt cgaccgcttg taaggcggct
     4141 tcgaccaatt ttccattgcc ggcaaggaat ggtttgcctg ataaattcca acttattcgg
     4201 tatttcaaac cgtgtttttc gagcatttcc gctactttgg ctttgatgat ttcatcggtc
     4261 acttcagtgc aataacgtaa gttaaactgc acataaagct cgcccggaat cacattatta
     4321 ctgcctgtgc cggctttgat attggcaatt tgcaaactgg taggcgggaa aaattcgtta
     4381 ccgttatccc actgataagt ggtcagctcg gttaaaaaac ccagtgacgt atgcaccgga
     4441 ttttccgcca aatgtggata ggcaacgtgc ccttgaatgc cctcgatata gagatctgcc
     4501 gtaattgaac cacgtctgcc gtttttaatc acatcgccaa acaccttgct actagacggt
     4561 tcgcccacta ccgcataatg taccgcttca ttgcgagcca ttaaggtttc aaccactttt
     4621 accgtaccgt ctttggctgc tgcctcttca tcggaggtaa tcaataatgc cactttgccc
     4681 ttatggtttg ggtgatgttg cacgaaagtt tctgccgcca caactaacgc cgagagcgag
     4741 cctttcatat ctgccgcccc tcgcccataa agcattccat ctacaatctc agccgaaaac
     4801 ggcggataag cccattggct ttcatcgcct accggcacca catctgtatg cccggcaaac
     4861 acaatgcaag gctctgtttt accattttcc gagccgtgcg ttgcccataa attcaaggtg
     4921 tcgccaaacg gcaaccattc aatattaaag cccagttttt caagacgttg tgcaatttct
     4981 tgctgacagc ctttgtcttg tgggctgatg gaatcacggc ggattaggtt ctgtgcaagg
     5041 gaaataattt catttttcat aggttacaag cggttgtttt gggtgaattt tttgctatat
     5101 atcctgtagg ggcgggtcct gtgcccgccc gaacaatcct aaagactacg gacaggcaca
     5161 ggacctgtcc ctacaagcag gggaaaaaat taattaaacg cagccacata ctctttctca
     5221 ttaaaaccaa tcaaagcaat atcctcttta atcacaatcg ggcgtttaat tagggtcggc
     5281 tgttcggtta gcacttttaa tgccgactca cggttaaggt tgtttttaat ttcctcatca
     5341 aggtttcgcc aagtggtgct acgtttatta actaagtttt cccaaccgaa ttttgcttcc
     5401 atttccacta accaatcggc atttaaaccg tccacacggt aatcgtgcag ttgtgggttt
     5461 tgattatgat cttcgagcca tttcaaggct ttttttactg tgtcgcaatt tttaatgcca
     5521 taaactaagg ttgtcatcat ttttcctcat tatctgattc cagcattgcc tgccagattt
     5581 ttaacgcatc ggcggtttct gccacatcgt gcacccgtaa aattttcgcc ccattttgag
     5641 cggctaataa cgcccctact acagagccaa gtagccgttg ttctaccggt ttatctaaca
     5701 ccgcaccaat cattgattta cgggaaagtc cggcaagcac agggaagcct gaatccacaa
     5761 aggctttaag gtgtttcaat aattggtaat tgtgtttcac gcttttacca aagccaaaac
     5821 cggggtctaa aataatgtgt tctttgggaa tgcctgcggt gatgccactc gaaaatccgt
     5881 tgatttaaga aatctgccac atcttccaac acatcatcat agtgcggatt ttgttgcatt
     5941 gtttgcgggt tgccttgcat atgcattaaa caaaccggca agcccaattc tactgccgct
     6001 gccaacgcat tcggctcggt taaggctcga atatcgttaa tgatatccat accgactttt
     6061 gccgactctt tgaatacttc tgctttggag ctatcaaccg aaatcaagca atcaaaacgg
     6121 ctgcggatcg cctccaccac cggcacaaca cgctctaatt cttgttctaa gctcacaatc
     6181 gccgcattag ggcgggtgga ttcaccgcca atatcaataa tgcttgcccc atcagctaac
     6241 attttttccg cttggaacaa ggctttatcg agggaaaaaa acttgcccga atcggaaaat
     6301 gaatccggcg tgaagtttaa gatccccata atttgcggtg tggttaaatc taacaagcgg
     6361 tcattttttt gaaaattttt gaaatggatt tgcatatttt tgcttatatt ttgttggtgc
     6421 aagcgtccac gcttgtacca ctctatgttg atttacagca caagcgtgga cgcttgcgct
     6481 atcttactct ctcattaagt tatttagtaa ttagccctta attttgccaa tacgcctttt
     6541 aaagttttat ccagcggggc ggtaatgacc atttcttcgc cggttttggg gtgttcaaaa
     6601 cgaatagcat gggcgtgcag aaataaacgc tgtaagccca cttctttcat tttgctgtca
     6661 aattccgcat caccatattt gtcatctaac gcaatcgggt gtccggcata ttgggtatgc
     6721 acacggattt ggtgggttct tccggtaata ggcgaagctt tcaccaaagt ggcattggca
     6781 taacgctctt caatgctgaa acgggtttcg gacggtttac cctcttcact cacccgtaca
     6841 attcgctcac cgctggcaag ttcgtttttt agcaatggag ctttaatcac tttggtatga
     6901 gcctgccatt gccctcgcac aagggcaaga tagtcttttt gtactacttt ttcacgcagt
     6961 tgttcgtgca aggaacgtaa cgccgaacgc tttttcgcca ccagcaaaat gcctgatgta
     7021 tcacggtcta tacggtgtac caattccaag aaacgagcct gtggacgtaa tgcacgcaag
     7081 gcttcgatca caccaaaact taaaccactg ccaccgtgta ctgcaatgcc tgaaggtttg
     7141 ttaattacta gcattgactc atcttcatac agaatttggc tctccagctc cgccactttg
     7201 tttaatttag tcgaaattgg ggcttcattt ttttccgcca cacgcaccgg cggcacacgc
     7261 actaaatcat caggctgtaa tttatattcg ggtttgtttc gtcctttatt cacccgcacc
     7321 tcgccttttc gcacaatgcg gtaaatcaaa cttttcggca cgcctttgag ctttgccaat
     7381 aaaaaattat ctaaacgctg tccggcttca tcttcactga tcgtaaaaaa ttgaacgccg
     7441 gcactaatta ctttttctgt tgtcattatc ttgtctttta gagaaaattt tgctctaaat
     7501 tctagcacaa aaccgcttag cgagtgaggc aaattcgcta ggcttatttt tcattttaat
     7561 tttaaggaaa caatatgact gaaaaagtca ccgaaacagc tccaaacatt gacgtggttg
     7621 ccgaaaccga aaaagtaatc cacaaagtca gtaatatgaa tattgaaact atgctaaatg
     7681 agtggattat cccttatggc accaaaattt tactggcgat tgcgattttt gtagtcggga
     7741 aatttcttgc ccgcctaatt agccgtttac tcggtaaagc cgcattaaaa tcaaccaaag
     7801 atgaaatgct gcaaagtttt attgtctcaa tcagctattt tttactgctg ttaattgtgg
     7861 tgattgcctc gctttcacaa atcggcatta acaccagctc gctggtcgca ttaatcggtg
     7921 ctgccggttt agccatcggt ttatcattac aaaactcatt acaaaacttt gctgccggcg
     7981 tgatgttgtt aatcttcaag ccatttcgca aaggggattt aatcgaaacc ggcggaatga
     8041 ccggcgtggt tgaacaaatg gggcttttag tgttggaact tcgcaccggc gataacaaaa
     8101 ccgtgttatt accaaacggc aaagtgtttt ctgacagcat catcaactac tctaacaacc
     8161 cgacacgccg tattgatttc acctttgata tttcttatga atctaactta aaagaggcga
     8221 aagagattat ccaacgcatt ttagacgcca atacttatgt attaaaagat ccggcacctg
     8281 tagtagcagt cggcgcttta gcaccgcata gtgttcaact tgttgttcgc ccttgggttc
     8341 agacaccaaa ttactgggca gcatattggg aaattatcga acaagtaaaa ttagcctttg
     8401 gtgaagcagg tatatctatt ccttacaacc aaatggaatt gcatattaaa agcggttcac
     8461 tagaaaataa agcataagaa aaaagcctgt aagcggtctt ttttcgataa aattttgcaa
     8521 attttgggcg aaggtttata ctttagctaa ataatctacg aacaggagca tattatgcgt
     8581 attgatacct ctgcactttg cgatctttat tctgaccaag ttgatgtggt tgaaccaatt
     8641 ttctcaagct ttggcggtgc ttcttccttc tttgggaaga ttaccacggt taaatgcttt
     8701 gaaagcaacg gtttaattgc tgaagttttg gaagaagagg gtgaagggcg agtgctgtta
     8761 gtcgatggtg gcggtgcggt tcgtcgtgcc ttaattgacg cagagctggc tcaacttgct
     8821 gtagataacg gctgggaagg tatcatcgtt tatggtgcgg ttcgccaatt agatattttg
     8881 gaaagattag atatcggtat tcatgcttta gcgccaattc cagtgggagc agataatacc
     8941 gacattggcg aagtggatac gcctgtgaat tttggcggtg taaccttctt ccccgaagat
     9001 tacgtttatg ctgacttaac cggcattatt ctttcgcaag agttgttgga tttaaacgac
     9061 atcagcgaat aaatatttgc aaaaaaatac caaaaaccga ccgcttggtc ggttttttac
     9121 tactgcagtg tttttaatgc cgcattatat tcttgagatt gctgcaataa aacccgagca
     9181 gcttcacgta atttatctac atcctcttcc ggcaaataaa atgatgttgc aatatttagc
     9241 acatcttttt ttaaatcacc atcaggtaat tcttttaaac ctaaactgac aaaatgcatt
     9301 tcgatctttg atggctgttt atttgcatat tcattccaac catctgtaaa ctggcgataa
     9361 cgttgtaaat taatttgtgt cgttctatca attggcacat taactattgt attcactaca
     9421 tcagccaccc ccggtacatt tgcggtctta tcgatctcgc tagatacttc attttgcgca
     9481 tttacattaa tcactacaat atttttcacc ctaagctgat gaatatactg gtacatctct
     9541 tcaataccca taacatcaga tatttccagc acactggcaa gccctaaatt atctgttaat
     9601 ccaccatcaa ctaaatggat aaaggggcgt tctttactat cttgataaag gctgagtata
     9661 tgattcgttt cttcaatatt ttttgtaatt agtccatcaa ttgctttttc tctagcacta
     9721 aattcttgtg gcatactaaa atggcaattt ccaccattgt tattcagtgt aagcggcgca
     9781 aaaatcagag gaacagaact agaagcagca acagcacggg aaatttctaa atcattcaaa
     9841 tttaaacaaa gcccatcaaa ggtttcttgc gtaaaggtca gtttttgacc aatgttcatg
     9901 tctgttgcgc taatcacagc aaaaggtcct ttacgatttt tcactaaatc accaaacttt
     9961 ttaccatgat aaagggcaaa atttaagcgt tcttgcaata aatcaccacg cccatattga
    10021 ggagaactta aacgaggaag attggataac gaaaacacct ctgtaataat atctttttga
    10081 aagttcgctt ttaaaaaacg gtcagcaaat ttaggaatga tatcctttcc ttccaacgca
    10141 aaataagtgg ctaatatcga tccacctgat acgccaaagg caatatctat attctctaat
    10201 aaagtattac ctttatttgt ttctttcacc cttacctttt taaactcttc caacactcca
    10261 taaccaagcg ccgccgctct tgttccacca cctgaaaaca tgatgacaac aatattaccg
    10321 tcttttgcac tttcagcgat cacatttttt tgtcgataac ctctttcaac atttacctgt
    10381 ttaattgttt caactggctt ataaacaacc aaactgcaag cagataacac tagcgtaaag
    10441 gcgaagatga aaagctgttt tattttgttt agaa
//
LOCUS       DANLUA010000083        10379 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLUA010000083 DANLUA010000000
VERSION     DANLUA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            357..1001
                     /gene="rppH"
                     /locus_tag="PXB44_001497"
     CDS             357..1001
                     /gene="rppH"
                     /locus_tag="PXB44_001497"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL5281969.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            1003..1791
                     /locus_tag="PXB44_001498"
     CDS             1003..1791
                     /locus_tag="PXB44_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5281970.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1799..2596
                     /gene="lgt"
                     /locus_tag="PXB44_001499"
     CDS             1799..2596
                     /gene="lgt"
                     /locus_tag="PXB44_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL5281971.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            2683..3351
                     /locus_tag="PXB44_001500"
     CDS             2683..3351
                     /locus_tag="PXB44_001500"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL5281972.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3421..3681
                     /locus_tag="PXB44_001501"
     CDS             3421..3681
                     /locus_tag="PXB44_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5281973.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3678..4010
                     /locus_tag="PXB44_001502"
     CDS             3678..4010
                     /locus_tag="PXB44_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL5281974.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4137..5168
                     /gene="trpS"
                     /locus_tag="PXB44_001503"
     CDS             4137..5168
                     /gene="trpS"
                     /locus_tag="PXB44_001503"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL5281975.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5232..5987)
                     /locus_tag="PXB44_001504"
     CDS             complement(5232..5987)
                     /locus_tag="PXB44_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL5281976.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(6043..6570)
                     /gene="ppa"
                     /locus_tag="PXB44_001505"
     CDS             complement(6043..6570)
                     /gene="ppa"
                     /locus_tag="PXB44_001505"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL5281977.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6787..6862
                     /locus_tag="PXB44_001506"
     tRNA            6787..6862
                     /locus_tag="PXB44_001506"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6820..6822,aa:Lys,seq:ttt)
     gene            6885..6960
                     /locus_tag="PXB44_001507"
     tRNA            6885..6960
                     /locus_tag="PXB44_001507"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6918..6920,aa:Lys,seq:ttt)
     gene            6982..7057
                     /locus_tag="PXB44_001508"
     tRNA            6982..7057
                     /locus_tag="PXB44_001508"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7015..7017,aa:Lys,seq:ttt)
     gene            7150..8184
                     /gene="mltG"
                     /locus_tag="PXB44_001509"
     CDS             7150..8184
                     /gene="mltG"
                     /locus_tag="PXB44_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL5281978.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8195..8815
                     /locus_tag="PXB44_001510"
     CDS             8195..8815
                     /locus_tag="PXB44_001510"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL5281979.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8815..9792
                     /locus_tag="PXB44_001511"
     CDS             8815..9792
                     /locus_tag="PXB44_001511"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL5281980.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9923..10165
                     /locus_tag="PXB44_001512"
     CDS             9923..10165
                     /locus_tag="PXB44_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5281981.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10158..>10379
                     /locus_tag="PXB44_001513"
     CDS             10158..>10379
                     /locus_tag="PXB44_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5281982.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIK"
BASE COUNT         3024 a         2274 c         2188 g         2893 t
ORIGIN      
        1 atttgaaaaa ctatcacatc tttatccaca aatggtgggg ataactctgc tttatttgcg
       61 aagcaaaaat tgccctgctt attcatttta aaatttaaac ttattttgac gagaaaaaac
      121 gcaacaactt gatctttatc cagatcattg cttaagaaaa agatcgcatt tttagatctg
      181 attaattgct ctgttttaca acgttccgcc tgtttatcct caacttatac cacttatatt
      241 caatagttat ccacaaagtt attcacaagc ggttatattt tatggttttt ttgcaaatca
      301 acccaaagta tgaaacaatt ctcccattct agaattttta tcaattaggt ggtcgagtga
      361 tcgatttcga tggctaccgc ccaaatgttg gaatcgttat ctgtaataag cacgggcaag
      421 ttctgtgggc aaaacggttt ggtcaaaact cttggcaatt tccgcaaggt ggaattaatg
      481 agggggaaaa cattgaagca gcaatgtacc gtgagcttta tgaagaggtc ggcttaagta
      541 aaaaagatgt acgattgctt tgggcgtcta aatattggct aaaatataaa ctgccaaaac
      601 gcttggtacg taatgaaggc tctggtcctg tctgtatcgg gcaaaaacag cgttggtttc
      661 tactgcaatt aatcggtgat gaaagcctga ttaatttgaa aaccacgaaa aatccggagt
      721 ttgacggctg gcgttgggtc agcttttggt atccagtccg ccaagtggtc tcttttaaac
      781 gagatgttta tcgcaaagcg atgaaagagt ttgcacaggt tttgcaaaac aatattcaaa
      841 aagcgactga cgataaaacc ggtgagaaac cacgcattga gcgtggcgag acttcgcccc
      901 atacttataa aaaaacctct caggattctg ccaaagccta taaacgagaa gctcgtaagc
      961 cttatcgtcc ttggcatcaa cataatcaaa aaggaaaata agatgactat tttattaagc
     1021 tgcctttttt taggggtgtt tgtcggcttt ttagccggct tgttcggcat tggtggtggg
     1081 ttaatcatcg tgccgagttt ggtcttccta ctgcccatgg caggtgtacc gccggaatat
     1141 ataatgtcca ccgcactcgg cacatcattt tcaaccatta ttattaccgc ctttgcatcg
     1201 gcacaacgcc atcataagtt aggcaatgtc gatatccaaa ccagtaaata ttttattcca
     1261 gcgttgatgg tttcggtttt tctatcgggc ttggtggtaa gccgcctcga tccgcaactg
     1321 atgtctagaa tttttgcggt aatggtactt taccttgcag gtaaaatgat tttttcaata
     1381 aaaagagcac cgaaaaataa accacttacc gttcaaacga cgataatcgg aggttcaatc
     1441 atcggtgcgt tagccagtat ggcagggatc gcaggtggag cctttattgt accattttta
     1501 aacgatcgag gcttagaaat gaaacgtgcc atcggcactt cctcattttg cggagcattt
     1561 ttaggtttat ctggcacaat cagctttatt gtaagtggct ggggcttaga aggaatgcct
     1621 gattattctc tcggttatgt ttatttaccg gctctgtttg gtattacggc aacttcattt
     1681 ttcacctcaa aaatgggggc aagtgccgcc aacgctctac ctgtgccgat gctgaaaaaa
     1741 gcctttgcgg tgcttttggt ctgtatcgcc atcaatatgt ttttaaaata aggacactat
     1801 gaacgaacaa tttatccaat ttccacaaat tagcccgatt gtgtttgaaa tcggtccgat
     1861 tgccctgcgt tggtatgggc taatgtattt agccggcttt gcctttgcct actggctggg
     1921 aatgagacgg gcgaaaaatt ccaatggagt ttggacaacc gagcaagtgg atagcttagt
     1981 ctatacttgc ttctggggcg tagtattagg tgggcgtatc ggcgatgtgt tcttttatag
     2041 cttcgaccgt ttcttacaag atccgctcta cctctttcgc atttgggaag gcggaatgtc
     2101 ctttcacggc ggtttaatcg gcgtcattgt agcaatgatt tgggtttcct accgccaaaa
     2161 acgcaatttc tggcagacag cagattttat cgcaccgcta gtaccgttcg ggctaggtat
     2221 ggggcgtatc ggcaatttca ttaacgatga actttggggg cgtgtaaccg atgtgccttg
     2281 ggcggtgtta tttccaagcg gtggctatct tccccgccac ccgtcacagc tttatgaagc
     2341 ctttttagag ggtgtggtgt tactctttat cctcaactgg tttattcaaa agcctcgccc
     2401 ggcaggtgcg gtttcagggt tatttttaat cggctacggc acattccgct ttattgtgga
     2461 atacttccga gaaatcgacc ataacgtcaa taccaccgca gatttaatca ctcgtgggca
     2521 actactctct ttaccgatga ttatcggcgg catactgatt atggcgtggg cgtataaagt
     2581 aaacaaagtg aaataaacgt aggggcaagt caatatttgc cctcattaca aaaattgcaa
     2641 aaaatttgca gaaaacgacc gcttgtgttt gaaggatata ctatgagtaa aaaatacaaa
     2701 gtcatcggtt tcgacttaga cggaacgcta atcaacaccc tccccgatct cactcttgtc
     2761 gccaattcaa tgtttctaga agccggtttg ccaacggtta cacaagaaaa agtgctgact
     2821 tggatcggta aaggggcaga tattttcttc caaaatgcgg ttgctcacac cggcagaatt
     2881 ttccaagccg atgagttggt gaaaatgcga gtgagctttg ataaatacta cgccacctat
     2941 gtgtgcgagg aaagcaccct tttcccaaat gtgaaagaaa ccttagaaat cctcaaagca
     3001 aacgactacc cgctggtggt gattaccaac aaaccgacca aattagtcga gccggtgctt
     3061 tccgctttcg gcatttatca tttattcgat gaaacccttg gtgggcaatc gctccccaaa
     3121 atcaaaccgc accctgaccc gatgtttttt atctgcgaaa aattcggcat taccccatca
     3181 gaattacttt ttgtcggcga ctcagaaaat gacgtgattg ccgccaaagc cgccggttgt
     3241 gatgtggttg gactaactta cggctataac tacaatgtgc cgattgaaca atctaacccg
     3301 acttttgtga catctgaatt taaagatgtg ttgaaaattg tggggttata aagaaaacat
     3361 ttgttttctt ttgatgtttg ctttagactt tccgaaccta aagatttaaa ggagtaaagt
     3421 atgccaacaa tcgcattacg tcaggtcggt ggctcggttg caatggtgat tccgcctgct
     3481 tttttagagg cactcaagct ctccgtcggt agcaaagtgg atttgtcttt aaacgacaac
     3541 caactcgtga ttaatcctgc ccgcccaaaa gtgaaataca gcttagagga attattaaga
     3601 caatccgatt ttgaggcagc aaggaatgaa gaaagccaat cgtggttatc cgagccggca
     3661 ataggaaaag aaattttatg aaacgtggcg atatttactg gctcgatctt gatccaacct
     3721 taggacacga acaatcaggc ttccgccccg ttgtaattgt ttccgcaact gcatttaatc
     3781 tagccagtaa actgcctgtg gttgtgccga ttactaacgg tggagctttt gctgaaagac
     3841 tgggctttgc cgtttcatta aaagatgccg gcttaaaaac aacagggatt attcgatgcg
     3901 atcaacctcg cacagtcgat attgcacaac gccatggaaa atttatcgaa gccttacccg
     3961 aaagcctact tgatgaaatt atggcaaggg tttcagtgat ttttgagtaa gatacaagcg
     4021 gtctgatttg ctctattttt tgcaaaatca tcaataaatc tgaccgcttg tacttttatc
     4081 ccttcccaat aaccgttaag ataacctata tttttcaaat caaacgagaa gataaaatga
     4141 gcaaacctat tgtatttagt ggcgtgcagc cctcaggtga attgactatc ggtaactact
     4201 taggggcgtt acgcaactgg gtgaaaatgc aagatgatta tgactgtgta tattgcattg
     4261 tcgatcaaca cgccattacc gttcgtcaag atccggagtc gctacgcaaa tcaactttag
     4321 atgtgttagc cctgtattta gcttgtggca ttgatccaaa caaaagcact atttttattc
     4381 aatcccacgt gccggaacac gcccaattag cttgggtgct gaactgttac acctatttcg
     4441 gtgaaatggg gcgtatgacg caatttaaag ataaatcagc acgctatgaa gagaatgtca
     4501 atgtgggctt atttacttat ccggtattaa tggcggcaga tattctgctt taccaagcca
     4561 accaagtgcc ggtaggcgat gaccaaaaac aacatttgga aatcacccgt gacattgcca
     4621 accgtttcaa tgccttatac ggcaaaaaag attccgaagg caacctgatc gagcctgttt
     4681 ttgcagtgcc ggaagtcttt attgcgaaat ccggggcaag agtgatgtcg ctacttgagc
     4741 cgaccaaaaa aatgtcaaaa tcggacgata atcgtaacaa tgtgattgga ctattggaag
     4801 atccgaaagc ggtagcgaag aaaatcaaac gggcaatgac cgatggcgat gaaccgccag
     4861 tagtgcgtta tgacgtacag aataaagccg gtgtctcgaa tttattggat attctctccg
     4921 ccattaccgg caaaaccatt gccgaattag aaaccgaatt tgaaggcaaa atgtatggtc
     4981 atttgaaaac agaagtagca gataaggttt ccgaactatt aaccaatctg caagaacgtt
     5041 atcaccacta ccgccaagat gaagcattat tggaaaaaat ctaccgtgaa ggggcagaaa
     5101 aagctcgagc tcaagccaaa gcaacgttag ataaggtata tgaattagtc ggttttgtgc
     5161 gttactaaca cttaatgaaa aatgctcttg aggattcaag agcatttttg caaaaaagaa
     5221 ccgattgtca attatttacg ggcgttataa atcttcattg cttccggcac aaggcgggct
     5281 aagttttctt gacggtcagc atttgatggg tgagttgaga aaatggacaa gcctgaatcg
     5341 ccattggctt tactcatttt tacccaaaca ttcggcgctg ctgatgggtt gtagcctgat
     5401 tctgccatta aaattaaccc aacttcgtcc gcctcagtct cttggctgcg agagaatggc
     5461 ttggtggcaa tcaaatccac gccagtgctg agtaaacctt cggtgcttac gcctgttgag
     5521 gcagtcacag ccacatctgc aatagcacct acaatactgg taatagcgct aaccgtacgg
     5581 tctgatttac cgtgttcttt cagtgcgtga gccatctcgt gtcccattac cactgcaatt
     5641 tcatcgttcg ataaattaag tctttccact aaccccgtgt aaaacgccat tttaccgccc
     5701 ggcatcgccc acgcattgag ttcatccgat ttcaatacgg taatttccca atgaaaaggt
     5761 acaccagtct ggttggctct ttccgcataa ggcttcattt tatgaaaaac agtgtgaatc
     5821 cgcttagcgg tggcagacga attgtcaatc gcattttgct gacgagcttg ggttttcact
     5881 tgagcataac tttgagccgc ttcttggtta atgctttctg ttgtggcaca agcggtaatc
     5941 actgttgcta aaaaaactga ataacttaat tttttgatga atttcatttt atatcccaca
     6001 aaaaatgcca aacaaatttg cttggcattc aaaaataaat aattatttct tagcacgctc
     6061 gaatgattct aagatttctt ggcgagcttc gtttacatcg ccccaacctt ctactttcac
     6121 ccatttgccc gcttctaatg ctttatagct ttcgaagaag tgctggattt gtgcttttaa
     6181 taacgccggt aagtcaccta catctttgat gtggtcatac tctttactta atttggtgtg
     6241 tggaacagca accacttttg catcaccgcc ggcttcatcg gtcattttta acacaccgac
     6301 aggacggcaa cgaatcaccg aacccggttg taacggatat ggtgttggca ctaatacatc
     6361 aaccgggtca ccgtctgatg ataaggtgtt attcacataa ccatagtttg ccggatagaa
     6421 cattgcggtt gccataaagc gatccacaaa taatgccccg gtttctttgt ccacttcata
     6481 tttgattgga tcagagtttg ccggaatttc aattacaaca taaatatcgt ccggtaattc
     6541 tttaccggca ggaactaatt ctaagcccat tttggtttcc ttctataata ggtgaaataa
     6601 acggcgtgat tatagcctat tttcagaaaa agataaaagc ccgtttctct tttcattttc
     6661 cgccaacaaa tccggctaat tttttgctaa attgcttaca aaccgcacac tcaaccattt
     6721 ttcgataaaa aaaacttgcc aaagttttaa aaatccgtaa gatatgccct cgttacgcaa
     6781 gattatgggt cattagctca gccggtagag cagcggactt ttaatccgtt ggtcgaaggt
     6841 tcgaatcctt catgacccac cattaatctt caaaacacct ctaagggtca ttagctcagt
     6901 cggtagagca gcggactttt aatccgttgg tcgaaggttc gaatccttca tgacccacca
     6961 ttaatttcaa aactcctctg agggtcatta gctcagtcgg tagagcagcg gacttttaat
     7021 ccgttggtcg aaggttcgaa tccttcatga cccaccattt actcaaatcc catttgcaaa
     7081 tttttctgaa aaaacaaccg ctttatagta gaattcagtc aatttttatc acttttattt
     7141 tattcatcaa tgctaaaaaa aatccttatt gcattaggct tagccggcgt tattgcttgc
     7201 ggtggactgt tttacggcta ccaaaaactc acctctttag ccgaacaccc gatcacggtt
     7261 cagccgaatc aacttttcgt attggaaaaa ggcgtttcca gccaaaaatt agcggctctg
     7321 ttagaagagc aaggcattgt cacccacgat gacgccgatc ttatccctta tttaatgcga
     7381 ttataccctg agttaagcaa attcaaggct ggtgcttatt cactcgcagg cttaacgaca
     7441 gtaaaagatt tgctcgctca tttaagctcc ggcaaagaag tgcaacttaa cgtgcaattt
     7501 attgagggca aaacttttaa aatttggcgg gaacaactag cgaaagccga ttatttacag
     7561 cacacgctac aaggcaaatc ggaaaaagaa atcgctgact tactcggcat tccacacgaa
     7621 aaaatcgaag gttggatagc cccggatact tacaactaca cgcccaattc caccgattta
     7681 gccttgctta aacgggctta tcaaaagcag aaaacggcgt tggaacaggc ttggaaaaat
     7741 agagcggaaa atctaccgct tgcaacacca tacgaaatgc tgattcttgc ctcgattgtg
     7801 gaaaaagaga ccggcattgc gaccgaacgc ccacaagtgg catcggtctt tattaaccgt
     7861 ttacgcaaca atatgcgact gcaaaccgat ccaaccgtca tttacggaat gggcgatcgt
     7921 tataacggca atatccgccg caaagattta gaagaggcaa cgccatacaa tacttaccag
     7981 attgacggct taccgccaac gccgattgcc atgccgagtg aagcttcgat taaagcggtt
     8041 gccaacccgg ataatacacc ctacctctat tttgtggcag acggcacggg cggtcataaa
     8101 ttcagcaaaa cactgaatga gcataataaa gcggtggcag aatggattca aattgaacgc
     8161 aaacgcaaaa atgaacaaaa atagtgagaa caatatgcgt ggaaaattta ttgtaattga
     8221 aggcttagag ggcgcaggca aaacgactgc tcaccaagtg attctcaaag agttggaaca
     8281 agccggcatt aatgacgtag taatgacccg agaacccggc ggcacccccc ttgccgaaaa
     8341 gttacgccat ttaattaaac acgaaaccga agaagccatt aacgataagg ctgagctgct
     8401 aatgctctac gctgctcgtg ttcaattagt cgaaaatgtg atcaaacctg cccttgccga
     8461 aggcaaatgg gttctgggcg accgccacga tatgtcttcc caagcctatc aaggcggtgg
     8521 acgtaagttg gatcgtcact tactcgaaac cttaaaagaa tcggtactcg gcacattcga
     8581 gccagattta acgatttatc tcgatctcga tccggtgatt ggtttagaaa gagccagagg
     8641 cagaggcgaa ttagaccgca tcgaacagca aagccttgac ttcttctacc gcacacgaga
     8701 gcgttattta gaattaaccc aacacaacga taaagccgtg atcatcaatg ccgaacaacc
     8761 catcgaacag gtgactgcgg atattcaaca agtaattaga aactggctga aataatgtac
     8821 ccttggctcg aaagcactta tcaacaactt accgattcat ttttacaggg tcgaggtcat
     8881 cacgccttac tgttcaaaac agatgttggt cttggtacag acaccttaat ctacaaggtg
     8941 gctgaatggt tgctttgcag ccaaaaactc cgccatcaac cttgtcttga atgcaaaagt
     9001 tgcctgctta tgcaaagcaa caaccacccc gatttccaca ttcttgagcc gattgaagac
     9061 aaagatattg gtatcgacca agtgcgtgag ttgatttata aactccagaa tttcgcccaa
     9121 caagggggaa atacggtcgt ttatattcga ggagttgatc gtttaaccga ggcaagcagt
     9181 aatgccctgc tcaaaacctt ggaagagcca cgtgaaaacg tctatttctt gctcgaagca
     9241 ccgcttcaag caacagtttt agctaccatt caaagccgca gccaaacttg ggtactttac
     9301 gccccacaag cctcagaagt aatggcttgg ctacaaacac aatttccggc aatctcaacc
     9361 gacgacttag agattgcact ccgcctttgc cactcacgcc cactaatttg caaaaatttt
     9421 atcgaaaatg accgcttgca acagcgaaaa acctttttgc aaaccttctg gcgtttctac
     9481 aaaagccacg atgtcttctt gttattccaa gccttcgccc aagaaaaaga gctgatttta
     9541 cagcaactcg aatggctgga aagtttcttt agcgatgccc taaaagctaa aatggcaatc
     9601 agccacggtt ggctaaaccc ggatctccaa aacggcattc tcccttttag ccaacaacta
     9661 acgacccacg gcttacttaa agggcatcaa attctccaac aaacccagcg ggatttaacc
     9721 gaaattaacg ccgtcaatcc ggaattgatg ttgctagatt gcctgacgaa gttggttttg
     9781 gtgtttgagt aaaagccctc tctcatagaa ggtagctttc aattagccgt aaaaagaggc
     9841 ttctagccct agaatatagt ttataacttg caaaattatc tatctttgtt attgtatgaa
     9901 taattcacac aaggagaata ttatggcaag aacaaccagc gtgactttag gcgaaccttt
     9961 aaatgatttt gtaaatgaaa tggtggcaag tgggcgttat ggctctacca gtgaagtagt
    10021 gagaaccgct ttacgaatgc ttgaggaaaa agagcaatac ttagctcagt tgcgacaagc
    10081 cattgatgat ggtgatgcta gtggttatgc aaccgaaacg attgatgaaa tgatgttgag
    10141 agtaaaggcg gaacttaatg tataaacttt ctgctcaagc actccgagat caagagaaac
    10201 tttttcgtta cagtatctct caatttggtg ttcaacaaac agaatcttat tttgcagatc
    10261 taaagcatat tttgaattta ttagctgatt ttccaacaat gggaacttct gcggaatata
    10321 ttcgtggtgg cttgcgaatg cacacacatc aatctcatac aatttattat cgaataaaa
//
LOCUS       DANLUA010000084        10231 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLUA010000084 DANLUA010000000
VERSION     DANLUA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            22..1284
                     /locus_tag="PXB44_001514"
     CDS             22..1284
                     /locus_tag="PXB44_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL5281983.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            1429..2205
                     /gene="deoC"
                     /locus_tag="PXB44_001515"
     CDS             1429..2205
                     /gene="deoC"
                     /locus_tag="PXB44_001515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL5281984.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            2226..2945
                     /gene="deoD"
                     /locus_tag="PXB44_001516"
     CDS             2226..2945
                     /gene="deoD"
                     /locus_tag="PXB44_001516"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL5281985.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(3021..3872)
                     /locus_tag="PXB44_001517"
     CDS             complement(3021..3872)
                     /locus_tag="PXB44_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL5281986.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            3987..4418
                     /locus_tag="PXB44_001518"
     CDS             3987..4418
                     /locus_tag="PXB44_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL5281987.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            4435..5115
                     /locus_tag="PXB44_001519"
     CDS             4435..5115
                     /locus_tag="PXB44_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL5281988.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            5179..6303
                     /locus_tag="PXB44_001520"
     CDS             5179..6303
                     /locus_tag="PXB44_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL5281989.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            6387..7004
                     /locus_tag="PXB44_001521"
     CDS             6387..7004
                     /locus_tag="PXB44_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL5281990.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNRF"
     gene            7147..8088
                     /gene="argC"
                     /locus_tag="PXB44_001522"
     CDS             7147..8088
                     /gene="argC"
                     /locus_tag="PXB44_001522"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL5281991.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            8180..9034
                     /gene="argB"
                     /locus_tag="PXB44_001523"
     CDS             8180..9034
                     /gene="argB"
                     /locus_tag="PXB44_001523"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL5281992.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            9047..10231
                     /locus_tag="PXB44_001524"
     CDS             9047..10231
                     /locus_tag="PXB44_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL5281993.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
BASE COUNT         2908 a         2053 c         2398 g         2872 t
ORIGIN      
        1 caatttataa ttagaggttc tatgggtact cttaacagct ttttaggcat tattgtttta
       61 ctattcattg catttttatt ttcgagcaat aaacgtgcaa taaacgtgcg taccgtatta
      121 ggcgcattag cattgcaggt agctattggt gcattggtgc tttatgtgcc ggcagggcgt
      181 gatgccttga atgcgatggc aacaggtgtg tcaaaagtga tttcttacgg taatgaaggg
      241 atttcatttt tattcggtgg cttagtcagc gacaaaatgt ttgaagtatt tggcggcggc
      301 ggctttgtgt tcgctttccg tgtacttccg gtgattattt tcttctctgc actcatttct
      361 ttgctttact acattggagt gatgcaatgg gtaatccgta ttttaggtgg tttattacaa
      421 aaagcattag gtacatctaa agcggaatct atgtctgcgg cagctaatat tttcgttggc
      481 caaactgaag ctccattggt agtaaaacct tatatcagca aaatgacaga atctgaactc
      541 tttgcgatta tggcaggggg cttagcttct attgcaggtt ctgttatggc tggttacgca
      601 ggaatgggcg taccattaac gtacttaatc gcagcctcct ttatggcggc tccggcaggg
      661 ttattattcg ccaaaattat ggtgccacaa accgaaaaat tccgtgatga actagaagcg
      721 gtagatttag aaaaaccggc aaatattctt gatgcagcag cttcaggtgc ttcttcaggt
      781 atgcaactgg cattaaacgt aggggcaatg ttaattgcat tcgtggcgtt aattgcatta
      841 gtgaatggta ttttaggagg tattggcggc tggttcggtt atgatacctt aaacttaggt
      901 caaattttcg gctgggtatt taaaccgctt gcgtgggtaa tcggtgtgcc ttgggaagaa
      961 gcagaaattg cggggcagat gatcggctta aaattagcgg taaatgaatt cgtgggttat
     1021 ttagagtttg ctaaatactt agctcctgag tcaaccgtac atttaacgga taaaactgtt
     1081 gcgattatta cctttgcact ttgtggcttt gcgaacttca gctcgattgc gattttaatc
     1141 ggtggtatcg gggcaatggc tccaagccgt cgtggtgata ttgcccgact tgggattaaa
     1201 gccgtcattg ctggctcact tgccaactta atgagtgcaa ccattgccgg cttgtttatc
     1261 ggcttgggtg gagcagttct ctaatccagt ttgtataaaa ccactagaaa tagtggtttt
     1321 atcttttttt atctgacgta aacggttgct tttggtttgt ctatttacaa gcggtcagat
     1381 ttttataaaa ttttaccaac caccacgcat ttaataaaag gagacactat gaatttaaaa
     1441 aaatccgctg agattgctct ttcgttgatg gatctcacca cattaaatga caatgatact
     1501 gatgagaaag tcatcgcatt atgccaacaa gcgaacacca agtttggcac accggcagca
     1561 atttgcattt atccacgttt tatcccgatt gcccgcaaag cattgaaaca gcaaggtatc
     1621 gagcaagtaa aaattgcgac agtcacgaat ttccctcacg gtaatgatga tattgaaatt
     1681 gcggtagctg agaccaaagc cgcagttgct tatggggcag atgaggtaga tgtggtattc
     1741 ccttataaag cattaattgc aggtaatgag caaataggtt tcgacttggt aaaacaatgc
     1801 aaagcagttt gtgctgccaa taatgtgttg cttaaagtga ttattgaaac cggcgagtta
     1861 aaatcagcag agcttattcg caaagcaagc gaaatttcga ttcaagcagg ggctgatttt
     1921 attaaaacct caaccggcaa ggtggcagtg aatgctaccc ttgaatctgc ccgcattatg
     1981 cttgaaacca ttcgtgattt aggtgtggca gaccgtgttg gctttaaagc tgcaggtggg
     2041 gtaaaaacct cggaagaagc cgcccaatat cttgcacttg cggaatctat tttaggcaat
     2101 gactgggtga gtgcatcaca ttttcgcttc ggtgcatcaa gtttattagc taatttatta
     2161 gcaacattga atgatgaaca gcagcaagct gttcaaggtt attaattaat aaagaggaac
     2221 ataaaatggc tactccacac attaacgcac ctgaaggtgc tttcgctgac gttgtaatta
     2281 tgccgggcga tccgcttcgt gctaaatata tcgctgaaac ctttttagaa gatgcaaaag
     2341 aagtgaccaa tgttcgcaat atgttaggct acaccggcac ttataaaggt cgccgcattt
     2401 cagtaatggg gcacggtatg gggattccat cttgctcgat ttacgctaaa gaattaatta
     2461 ctgaatatgg cgtgaagaaa attatccgtg tcggctcgtg cggtgcggta cgtaatgatg
     2521 tcaaagtgcg tgatattgtc atcggtgttg gggcttgtac cgattctaaa gtaaaccgta
     2581 ttcgcttccg tgataacgat tttgcggcga ttgctgattt tgatatgaca ttagctgctc
     2641 accaagcagc taaagcgaaa ggcattgaag ctcgtttcgg taatttattc tcagccgatt
     2701 tattctactc acctgatgtc gaaatgtttg atgtaatgga aaaatacggc attttaggcg
     2761 tagaaatgga agcagcaggc atttatgcgg tagcggcaga gtatggagca aaagcattag
     2821 caatttgcac cgtttcagac catatccgca ccggtgagca aactacctct gaagaacgtc
     2881 aattaacctt taatggtatg attgaaattg cgttagagtc agtattaatt ggcgataagc
     2941 agtaatttct aacctgacaa gcggtgaaat tttgcaaaaa atttgcagaa tttgaccgct
     3001 tgttattttt gattcttcct ttatcttcct ttcgggcttt cgccaaactg ccttttatat
     3061 tctcgactaa actggcttgg gctttcatag ccgacttgat aggcggtttc actcacgctt
     3121 actcctgctt gtagtttttc ccttgccgca tttagtctta acgatttctg atattgcagt
     3181 ggtgtcattg aagttacgct tttaaaatga gcgtaaaacc ccgccacgct cattcctacc
     3241 tcttcagcca gtgctgacat atttagtgta tcggcaaatt ttctttctaa gatttttgtt
     3301 gcattggcaa ttttagcaat attgccatct tgagagagta gagagcgtat atagccacct
     3361 tgctcagatt gtaataaata gaaatagagt tcgtttaata tcaatggtgc aagaaaatga
     3421 atatgatttg gagaattaag caaatttagt aagcgtaaca aacagtcttt aatttcattc
     3481 tccagctccc atttagtgcg ttgattaatg ttttcactta ttttctgtgg gggaagctgc
     3541 gtgataattt tggcaataag atcaaaatcc aagcgtagtg aaatcgcaag ataaggctga
     3601 ttttctgcaa ctgataaggt ttgggtaaca atcggcaaat tcacagggca aaacatcatt
     3661 tcctgattac cgaagacttg gcaatgtcca ttggtacaga tttgccgctc gcctaataat
     3721 actacacata aacttgggtc ttggatataa ccgttaaatc taatagaacg atcggcagcg
     3781 tgaacggtta gccctgaaat gggagtagcc caaagttcat ttttcggagc gagggcaaaa
     3841 atagattcaa taatagctcg atgagtttgc ataataatct cacttttaga aaattaggca
     3901 tattttatag aaaatcaggc aagacgacaa atagcaaaaa gcggataata tccccaatca
     3961 aataaagcat ttaataggag caaaatatga aaatttttta tcacacatca gcaactgcaa
     4021 ccggtggtcg tgacggcaga acccaagtgg atgacggttc tatcggtttt gatttagtcg
     4081 gtttccaaaa tgaaagtggc aaagtaggca ctaatccgga acaattattc gcaatgggct
     4141 atgctgcctg ctttgacagt gcaatgaatc acgttgcccc aaccttaggc attaagccaa
     4201 ccaaatccag taccactgtt gcagttggga ttggtcaaaa agctgatggt gctttcggct
     4261 tagatttaga tattaccatt actgtggaag gcattagtgc ggaagaagcg aaaactttaa
     4321 ttagcaaggc acacgaagtt tgcccatatt caaatgcaac ccgtggtaat gtagatgtgc
     4381 gtttacacgt taaagtcgtg agtgaatttg acttataaaa aataaatagg ggaaatgaaa
     4441 atgttagatt ttgaaaccac ggtaaaaaca cgacaatcaa tccgcaagtt tttaccgctt
     4501 ccgatgacgg aaactgaaat caaacaaatt cttgccgatg cacaaaatgc cccttcggct
     4561 tgtaatacac agccgtggtt agtacacgtt gcctctggtg aaacgctagc acgcttaaaa
     4621 gctcgtttta aaaagacttt tgaagcgggc attaatgatg cggattttga gttcaatcaa
     4681 acgaaattta ccggtgacta cgaaccacgt tggcgtaacc aatacagcca cgtttttacc
     4741 accagctatg gcattccccg tgaagataaa gccggtcgcc aagtggtgta tgatgacaat
     4801 attgtcggtt atggtgcgcc acatatggca ttcctattta tgccaagcat tgacggacac
     4861 gatgtcaata ttgccagcga tgttggtatg tattcgcaaa ctttcttgct ctcactcacc
     4921 gctcgtggtt ttggcggcat tccgcagctt gcccttgcga tgtttgctaa agatgtgcgt
     4981 gaagagttag gcattcctga acattacaag ctcctccacg ccatttcttt cggacaccca
     5041 gattgggacg ctatgcagaa caaacaccat ttagggcgtg tgccggtgga agagagtgcg
     5101 accttacatt ggtaataaac aagcggttga tttttcgcaa aaatttgcga aatcaaccgt
     5161 attttttagg agaatacgat gataaattta tttacccctt atactctgaa taacgatgta
     5221 gaaattaaaa accgccttgt agttgcccca atgacccatt ccgcctccaa cgatgacggc
     5281 acaattagcg agcaagaacg ccatttcttg cataatcgtg cagcagatat gggcttattt
     5341 atcaccgcag caaccggggt aatgcgtaac ggcaaagcgt tttacggtca gccggaagct
     5401 cttgatgaac gctatttggc aagcctgaca gaaactgcca atatcttgaa aaatcaaggc
     5461 accaaagcga ttttgcaaat tttccacggt ggggcacaag ctattcctgc cttgcttgac
     5521 ggggcgaatg cagttgcgcc gtccgagcac gccaacggtg ctaaagccct aacggcggac
     5581 gaagtgaagc aaatgatcca agctttcgct aatgccaccg atttagctat tcaagccggt
     5641 tttgatggtg tggaaattca cggggcgaac ggctatttaa ttcaacaatt tttctctact
     5701 gaatttaatc aacgcactga tgaatggggc ggtagccttg aaaaacgctt gcgtttcccg
     5761 ttagcggtgg ttgaggcagc ggtcagcgtg cgtgaaaaac accaacgccc tgattttatt
     5821 atcggctacc gtttctcacc ggaagagccg ggcgaacacg gcttaaccat gaccgatacc
     5881 tacgccctga ttgatgcttt gtgcgaaaag ccgttgcaat acttgcacat ttcactctgg
     5941 gatttcggca aaaaagcccg ccgtggtgca gataccaact tcacccgtat gcaactggta
     6001 caccaacaca tcaacgggcg tttaccgctg atcggcgtgg gtaacttatt aaccggtgaa
     6061 cagatccgta aagcttatgc aacaggctgg gcagaattta tcgccttagg taaagcggta
     6121 atgctcaacc cgaacgtggc gaccttgttg aaagataatc gagacagtga gattgtcagc
     6181 gaactcgacc cgcaacaagc cgaccactac ggcattcccg atagactatg gggctactgc
     6241 gtgcaaggtg gtgcttggtt accgccgctt aaagggcaaa acgggcaacc aatggatatt
     6301 taatcattac agacaagcgg tcattttttt gtaaaaattt gcaattaagt gaccgcattt
     6361 tttcaattat ctgaaggaga aaaattatga ttacgttaca ttacctgaac agttcctgct
     6421 ctcaccgcat cgtttggttg ttggaagcct taggcttgga ttacgagcta aaaatctacc
     6481 atcgtgaaga gagcggtttt gcacctatgg aggtaaaagc cttgcaccca cttggcaaag
     6541 cccctgtgtt gcaagatggc gatttggtgc ttgccgaagg caatgcgatt atccagcatc
     6601 tgattgcccg ttacgccgaa gggaaatttg agccgactcg tggtagcgaa gcctattcaa
     6661 tgtatcacta ctggttgtcg atttctgctt cattattctc ggcaaacctt gtgacaatgt
     6721 tggctaatcg ctctgatctc ggagcttttg gcgactacgc aaaagcgcag actccgctgt
     6781 attttaacca tattgagcaa acactttcag ataaaaaatg gattgtgggt gatgaactaa
     6841 ccggtgcgga tttttctctc tgtttcccac tacaatgggg gcgtaatttt gttgaaatgg
     6901 caaattaccc gaatattgca cgctatttag aacaggtgga aaatcaccct gcttaccaaa
     6961 aagcgaatca aaaagccctg aagcttgata tgaatagatt ttaatactct ttttaatccc
     7021 taacattttg ttagggattt ttgaatataa aatcaattaa tgtgaataat tattcaaaaa
     7081 taaacttgag tttagcctta aatagcctta gactaccgag cagaaaaagt caaagaagga
     7141 ttttcaatga gcaaatacaa gatttttgtt gatggggcag taggaacaac cgggctacgc
     7201 attcacgaac gtttaagcca agcggacgat attgaattat taaccttgcc cgaagagcag
     7261 cggaaagatt tgaacgcccg tgtggcaaaa gtcgcagagg cggatttaac tttcctctgt
     7321 ttgccggatg aagcctcaaa agaattagta gcaaatgcac cggaaaatac ccgaatttgc
     7381 gatacttcaa ccgctcatcg ggtcaatcct aattgggttt atggttttcc ggaattgagc
     7441 ggacaacgtg aaaaaatcca aaatgccaag cgtgttgcgg tgccgggttg ccatgccacg
     7501 ggctatattg cgttggttcg tccactggtg gaaaaaggga ttctgcccgc cgattaccct
     7561 ttaagttgtc attccttaac cggctattca ggcggtggca aagcgatgat tgctgactat
     7621 gaaaatgaaa acagcccaaa tgctttcaat gcccctcgtg tttacggttt aactctccaa
     7681 cacaaacatt tgcctgaaat gcaggcagtt acccaaatgc aaaacccacc gattttcacc
     7741 cctgtggttg ctgattatta cagcggaatg ttagtcactg tgccattgca gatcttcgcc
     7801 ttacaagcgg tcaaatccgg cgaagaaatt gcaaatttgc tcgccgatta ctaccaaacc
     7861 agccgactaa ttcacgttca ccctttccaa tctctacccg aagacggaat gcttgccgcg
     7921 aatgcttatg caggtaaaga cagcttagag atttttattt acggcaacag cacccaactt
     7981 ttacttgctg cccgttttga caatttaggc aaaggggcat caggtgccgc agtgcagtgt
     8041 atgaatatta tgttgggaag agatgaggtt gaggggttag agctttaaat ataaatgtag
     8101 ggacaggtcc tgtgcctgtc cgataatcat cgcccatatt cggacgggca caggacccgt
     8161 ccctacaaga gaaagaaata tgcaacaaca agaaatcaat caatttgcaa atttattaga
     8221 aaaatccacc gcttgtttgg tgaaatggca gggtaaaatc attgtggtga aatatggcgg
     8281 taacgcaatg attaatgagg aactgaaaaa atcagtgatg gcagatctgc tgttacttca
     8341 tcgtttaggc gtaaaagtgg tactggtaca cggtggcggg ctggagattt cacaaggcgt
     8401 gaaattattg ggtaaagaac cgcaatttat caacgggttg cgtgttaccg atagcgatac
     8461 gattaacgtg gttttgcaaa tgttggcagg caaagttaat aaaagccttg tcgccttatt
     8521 aaaagggaaa ggcattgggc tgtgtggtat tgatggggcg atgttgcaat gcaaaaaatt
     8581 agaggcagag gtggattacg gctttgtggg cgatattgtg aaagtcgatc caactgtgat
     8641 tgaaattgct cttaatgcgg gcttgattcc ggttgtttcc acggtgggcg ttgatgaaaa
     8701 cgggcaggct tataacatta atgcagacac cgctgccagc gagattgcga ttgcgttaca
     8761 agcggaaaaa ttggtctcga tgaccgatat tgcaggttta ttgcgagatc gctttgatga
     8821 aagcacctta attccggaag tggaagtaag cgaagtgcag tccctgattg agcaaggcat
     8881 tattgccggt gggatgatcc ccaaaattgc gtgctgtacc gatttcatca gagctggtgg
     8941 ctctgaggca aatattattg acgggcgaat cgaccacgcc attttggtag aaatgttcgg
     9001 tggcaaaaac ggcacacggt tttataaaaa ataacaggaa agtgaaatga caagcagtaa
     9061 tacgattaag caattagata aggattatat cgcccaaacc tatggcagat ttgaccttgc
     9121 actgtcgcac ggaaaaggct gtgaagtgtg ggattttgag ggcaataagt atcttgattt
     9181 taccagtggc attggggtaa atagtttagg ctgggcggat gaggattggc tacaagctgt
     9241 taccacacaa gccggcaaat tagctcacac ctcaaacctg ttttttaccg aaccggcaag
     9301 ccaattagcc caaaaattag tggctgcaag cggtctaaaa cgggcatttt tttgcaattc
     9361 aggggcagag gcaaatgaag gagcaattaa aaccgcccgc aaatatagtt tcgacaaata
     9421 cggttccggt cgttcaacgg ttttaaccct cgtgaactct tttcacggac gaaccatttc
     9481 aaccctcgca tccactgggc aagaggtatt ccacaagcat tttttcccct ttacagaggg
     9541 ctttgaatat ttacctgtaa atggcattga ggcactgaaa gctcgcttgg aaactgagaa
     9601 cgtttgcggg attttgctgg aggtggtgca gggcgagggc ggtgtttgcc cattagaggc
     9661 ggattatctg cacgccgtgc aaagcctgtg ccaagaaaaa gatattttat ttatgattga
     9721 tgaggtgcaa accggcattg ggcgaacggg cacacttttt gcttatcaac aatttgattt
     9781 aactcctggt ttagtcacgc tcgctaaagg cttgggcggc ggcttgccga ttggtgcttt
     9841 cctgttaggc gaaaaagtgg aaatgacatt aggcaaaagc gaccacggct caacctttgg
     9901 tggcaatcct gtttcctgtg ctgctgcaaa tgtagtaatc gataaaataa acgccgaatt
     9961 tctcgcccaa gtgcaacaaa aagggcagtt gttacagcaa cgcttagcga gcttaccaaa
    10021 agttaaatcc gtttcaggct taggtttaat gctcggcgta gaatttgagg aaggcatttt
    10081 agccgctgat attgttgcaa aatgtatcaa caaaggtgtg ctgtttttaa ccgctaaaag
    10141 caagttaagg ctactcccac cgctgatgat ctctgaacaa cagatagaac aaggaatcag
    10201 agtgttagag gatgtcttga aagattcttg a
//
LOCUS       DANLUA010000085        10229 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLUA010000085 DANLUA010000000
VERSION     DANLUA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..576
                     /locus_tag="PXB44_001525"
     CDS             <1..576
                     /locus_tag="PXB44_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL5281994.1"
                     /translation="GAYLEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTA
                     AVKCWVFNGDLYIEFEAGKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKR
                     HGLPRIIGVEKWKGSVEDGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLP
                     VVIDAHNHFIDALRYALTPLMQVKSAKGVLL"
     gene            573..1949
                     /locus_tag="PXB44_001526"
     CDS             573..1949
                     /locus_tag="PXB44_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL5281995.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            1903..2841
                     /locus_tag="PXB44_001527"
     CDS             1903..2841
                     /locus_tag="PXB44_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5281996.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            2828..4198
                     /locus_tag="PXB44_001528"
     CDS             2828..4198
                     /locus_tag="PXB44_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL5281997.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            4191..4625
                     /locus_tag="PXB44_001529"
     CDS             4191..4625
                     /locus_tag="PXB44_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5281998.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            4640..5629
                     /locus_tag="PXB44_001530"
     CDS             4640..5629
                     /locus_tag="PXB44_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL5281999.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            5641..5937
                     /locus_tag="PXB44_001531"
     CDS             5641..5937
                     /locus_tag="PXB44_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282000.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            5940..6317
                     /locus_tag="PXB44_001532"
     CDS             5940..6317
                     /locus_tag="PXB44_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282001.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            6317..6661
                     /locus_tag="PXB44_001533"
     CDS             6317..6661
                     /locus_tag="PXB44_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282002.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            6663..7031
                     /locus_tag="PXB44_001534"
     CDS             6663..7031
                     /locus_tag="PXB44_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5282003.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            7028..7408
                     /locus_tag="PXB44_001535"
     CDS             7028..7408
                     /locus_tag="PXB44_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282004.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            7412..7894
                     /locus_tag="PXB44_001536"
     CDS             7412..7894
                     /locus_tag="PXB44_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282005.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(7934..8350)
                     /locus_tag="PXB44_001537"
     CDS             complement(7934..8350)
                     /locus_tag="PXB44_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5282006.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(8406..8582)
                     /locus_tag="PXB44_001538"
     CDS             complement(8406..8582)
                     /locus_tag="PXB44_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5282007.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            8678..9349
                     /locus_tag="PXB44_001539"
     CDS             8678..9349
                     /locus_tag="PXB44_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282008.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(9364..9597)
                     /locus_tag="PXB44_001540"
     CDS             complement(9364..9597)
                     /locus_tag="PXB44_001540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282009.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            9766..10224
                     /locus_tag="PXB44_001541"
     CDS             9766..10224
                     /locus_tag="PXB44_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282010.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEVSDSMFNAMQNRYCK"
BASE COUNT         3063 a         2108 c         2429 g         2629 t
ORIGIN      
        1 ggtgcttatt tggaagcgag cgaagcgcaa atctttcgcg gtaaatatca agaaatggaa
       61 tttgtgccga accctgattt tgatggccca tattacggct tagattttgg tttcgcgcaa
      121 gatccgacgg cggcggtcaa atgctgggta tttaatggcg atctgtacat cgagtttgaa
      181 gcaggcaaag tgggcttaga acttgacgac accgcagact ttattgccaa aggcgtgcca
      241 caaattgccg atcacgttgt gcgtgcagat tcggcgcgtc ctgagtcaat tagttacctc
      301 aaacgtcacg gcttaccgcg cattatcggt gtggagaagt ggaaaggctc ggtggaagac
      361 ggcattgagc atattaagtc ctacggcaaa gtctttattc acccacgctg tcagcaaacg
      421 ctcaacgaat ttcgccttta ttcgtacaaa accgaccgct tatcgggcga tgtattgcct
      481 gtcgtgattg atgcccataa ccactttatt gatgcgctac gctacgcatt aacgccgttg
      541 atgcaagtta aaagtgccaa aggagtttta ctatgagcct agagcaagac agactggctt
      601 ttttagccga agccctaggg cttggcaata tcaaacgaag aacgttatgg aaggagtttg
      661 gctaccctaa taccttaaca ttcaaccatt tcttaaaagc ctataagcgc aatagtatcg
      721 cttttgcagc gattaaccgc ttacttgacg gttgttgggt tgactgcccc gtaatcgttg
      781 agggtgagca aaagaacgaa tcaaagcaga caaccgaatg ggagtccaaa gttgaacgtt
      841 ttatgaaacg ctactggtcg gcaatcaaag aggcagatag acgaaacctt gtcggtaact
      901 attcggcgtt attacttcaa gtaagagatg gtcaacaatg ggatcagcct attttgcaag
      961 gcgcgctatc tagcattgga gagttcggac tggttaaact gatccccgtg tggcagtcac
     1021 agctttcagt gacagagttt caagaggatg tgaccgccga taattacggc gaaccgctga
     1081 tgtatcaatt cagcgaatct gcctttggca aaaaaagcgt gtcacgaaat atcaaggttc
     1141 acgcaagtcg tgtaattttg ctcaatgagg gtggcgactt taattcccct cattctggag
     1201 ttcctctgct tgaggcgggt tacaacaagc tagttgatct cgaaaaaaca tcgggcggca
     1261 gcgccgaagg ttttctgaaa aacgcaagcc gacaattagg gatcaaatta acaaaagatg
     1321 ttgatttgag acagctcgaa gacagcgcaa aagcccttgg gttttcttca ttttctgatg
     1381 cgttgaacga caagatcaag aaaatcaact caggaacaga ctcagccttg attactcacg
     1441 aaggtgatgc ctctgtgttg tcagtcgcac cagcagatcc gaagccaacg tgggaaattt
     1501 ctgcaaatga gtttgccgcc tcagtacaaa tccctttcac gattttgttc ggacagcaaa
     1561 cagggcgatt ggcaagtgac gaggacaaaa ccgactgggc aaaccgttgc aacggtcgtc
     1621 gaaatggttt tctcaccgat gtgataacac aactacttaa tcgcttgtgg ttcatcggtg
     1681 tgctacctat gccaaaaaat agcgatgtca cagtgtcttg gtctgactta cttgcaccaa
     1741 gcgaaaaaga gaaaatcgca aacgcacagg ctttggcttc agttgcaact acatcgcagt
     1801 ctgcctttgg ttttgccgcg gtaacagcga acgaaatccg cgaatcttta gggtttgaac
     1861 ctctgcctga taatctgatt ccgccaacaa ttgacgacga aaatgaagat caagacgaaa
     1921 atcaagccac tgcggattcc gacaaataaa gccgatccgc tcaatatggg agtgtcggtt
     1981 gccaagctat ttcgtcacat tgatgtcatt tactcacaaa ttaagcgctc cattaagcaa
     2041 caaatcttgc aaaagatgtc actgcgactg caagcaaata attttgagta ttttccttcg
     2101 tatcttactg cgactgagtt ggcggatttg cttgaaacct tgcaaaagat tatcgacaaa
     2161 gagattttgt cgaatggttc tcaaggcgaa aatctctggt ttgactccta tatcgacgaa
     2221 gcagcactaa aaggcactca gtctgcggtc actgatttag gtctgcaatc agaggcttat
     2281 cgcagtcagc gaaacttatc atcaatcgtt ttcagtcctg cgtacttcaa ccgattggcg
     2341 attgcacaca tggcaagtta tagcgagtgg caaggtttaa gcgacgagtt gcgaaaatct
     2401 ttggcggggg tgatcacgga ggcggtttta aacggcaaaa acgtgaaaga aactgcccgt
     2461 gagattcggc aaaaacttga tgtgtcatca aaacgggcga agcgaatagc gcaatcaagc
     2521 cagttggcgg catatcgtcg ggcagaatgg caagaggcgg aagaggcaaa agaagaactt
     2581 gggctaaaca ccaagctctt acatttttca gcgctcaaag ctactacgcg attagcacac
     2641 gcccaacgcc acggcaaata cttcgatgtt gcagaggtgc gcgaatggta tagccgagac
     2701 ggcaaccaat tcaactgtta ctgcaagcag tctgtggttg tggtcaacgc cgacggtaaa
     2761 acagatattg agcctctcct tgttgggttg gctaaagaac gtaaacaatg ggtaggagca
     2821 ttaaaaaatg tcaaaaaata acgtgaacat cgtcaccgtc attaacagca aaaatatcag
     2881 cacagaatct attgacggag atcaacacct gattatccgc ggcgttgtgc cgattgttga
     2941 tgatgttgtg atgaacgacg gactttatcc tgcggatgaa atcaataaaa gctacaacag
     3001 cttagagggc aactttatgc cgcttgggca tccaaaaatc gaaggtaaat acgtttcagc
     3061 ccaagatgtt cgagcggtga acaaacatca cgtcggtgcg tgggcgaaga acgtacgcaa
     3121 ggaaagtggc aaagtgctgg tcgatatgta tatcaaccgc cgctttgcgg aaggttcaga
     3181 acagggcaaa accttgcttg aacgacttga cggaatggtg acaaacacca acgctgaacc
     3241 tattcacgtt tccacagggc tgattctaaa caaagtcaag caatcgggta aatccaaagg
     3301 caagaaatat agctggattg cgacaaatat gtcgttcgat catattgcaa tattgcttga
     3361 tgtgccgggc gcggcgacac ctgatgatgg cgtggggatt tttgtaaacc aagacggaag
     3421 cgaaatgggg gtggaaaatg taaacctaac acaagcggcg gattaccgca cagaaaagct
     3481 gtttgacaaa gtacgatttt acctgatggc gaactcagat ttatcgtttg aagaaattca
     3541 tcgcgcgctg cgtgaagcta ttcgccccga aggcaaagat aagtggtcat tttgggtcga
     3601 agctgtttac ccgaaatact tcatctattc ggacgataac agcggcaaga aatacaagcg
     3661 gagctatttt attgacgaaa atgacaatgt gcagctcact agcgaacctg tcgaagttgt
     3721 caagaagatt ggctacgaag aaattacaac caatgaggaa cataatccaa tgaaaaccaa
     3781 aatcttagcc gtgttgaatg cggcaaacgt gaaaacggaa ggcttaaatg acgatcagct
     3841 cttggaagcc tacaacaagc tgcaagcgga taagggtaaa gagcctgaaa agcctaaaaa
     3901 tgaagatgac agcgaaattg atgagaaaat caagaaagca gtcaatgccg cgattgagcc
     3961 actccagcaa gccttgcaag ctaatactga caaagagcaa gcagcaatgc gtgcggcggt
     4021 gaaagccaag ttcaatatga gcgatgccgc agtcaatgct ttgcaaggcg aggtattaac
     4081 tgagttgtat gcgcaaacgc aacaatcggt cggattaaac aataattcgg caaacggcaa
     4141 cggcgacaat caatgggatg gttactcact caaccaagag gagaaaggca atgagtaatg
     4201 taatttatcg cggttcggtc aagcgtgaac ctcaaacggt gaatatcgtg atcaacgata
     4261 cttcggcacc gggtgcagta gttaaactcc acaacggcaa attagaggcg gcggctgatt
     4321 taaaaggtcg ccgtttttta ttgggcaaca accgctttaa ggggcagaca atcgaacaag
     4381 cgtatgcgaa gggcgatact gcaacggcgt ttcgtttaga gcctgaacag gagtattacg
     4441 cgcaaattgc cgacggcact tataactttg gcgatgaact cacggtaaaa caagcgggcg
     4501 gcaaactgac caaagcggag acgggcgatg tagtgctatt tttcttcgac gaagaatcgc
     4561 agcgtgtaat cagtggcggc aaaggctatg ccgatgtcgt ggtggcaaac gcctacacta
     4621 aataagaggt aacaaagcaa tgttgaaatt cacaaaagaa caagaacgct tcgtgattaa
     4681 cgagcggatc aattacgaca aaaaacacgc gctaatggca gcaaattcgg cggggttatt
     4741 gggcaacgcg atgactttgc cgaaagatgt ctgggcggaa tgggatcgtg atgcagtgca
     4801 aattcagcgc gaagaattgg tggtgtttaa cgatttagcg ggcatttcaa aatcaatgcc
     4861 gattggcaaa cttattcacc atttccaaac ggtatctgat agcggttcgg ttaatatctc
     4921 attagacggt cgttcatcag cgaaaaccga taacgtggtg cttaactacc acggtacacc
     4981 gttgccgatt attgattcca ctttctcttt tggctggcgc gatatgtcgg cagctcaaag
     5041 cgaagggtat cagatcgacg gtgcagcacg cgcgaacagt ctccgcaagg tggctgaaaa
     5101 aatggaagat ctcgctttaa atggtgattc ctctattgtg gttggcaatg ccaagcttta
     5161 cggcttgcgt actgcgccaa atcgtatgac taacacacac aatctggatt tagccactgc
     5221 gacaccgaaa gaaatttacg atgtgttccg tgacttgatt agcaaattcc acgcgaagaa
     5281 ctactactct cctgtaacgc tttatgtgaa ctatggcgac tatttcgcaa tgagtacacg
     5341 tgattactcc gagcagaaat ccgaaggatc tatcttgaac aaagtgatga cgattccgca
     5401 aattgcgaaa atcgtgccag caagccgtgt accgcaaaat gaaattctcg gcttgtgcaa
     5461 gcgttcggat gtgtaccaag tgcttaacgg tatgccgtta gtgactcgtc cgattgcgcg
     5521 ccataatgaa accgatgatt actcgttcca aattatggca gccgttgccg tggagttcaa
     5581 attcgacgat aaaggcaatg ctggctatat ccagtacact aaatcctaag gcggtgcaaa
     5641 atgaagtggc tattaacaca cgatagccac gagttgaaga aaggcgatgt ttacgaaggc
     5701 gaaacattac cgctttggct tgtgggcaaa gctatcgaaa tcgaagaaaa atcctttgaa
     5761 gttgcaacgc cttctgatca cgctgacggc gaaactggcg aaactggcga aactggcgaa
     5821 actggcgaaa ctggcgaaac tggcgaaact ggcgaaactg gcgaaactgg cgaaactggc
     5881 gaaactggcg aaactggcga aactggtcag aaaggcaaaa aagataaagg caaataacta
     5941 tggcagcaga aatagagcta tccgaagcgc gaacaacgct tgacgaactg ggtttcactt
     6001 cgccgaactc tctgcttgag ctttacgttc agcaaacgaa caccttagac caggcgttga
     6061 acaaatcggg gtacagcgaa gaaacacaga aactgatcaa gctctatctg gttgctattc
     6121 ttgcaatttc atctggggcg cgccgtataa agtcagaagg tgcgcctagc ggtgcaagcc
     6181 gaacgtttga gtatgatcca aacgcgctag acaaccttaa gtcggcgatt cgctcaattg
     6241 atccgaaagg ttgcgctgaa agtttgctac ccaaagataa agttgtcggc tttttcgatg
     6301 tggtcggagg ctgctaatgt caaatttatc gaattggtcc tacaccgcaa aagccacgct
     6361 ttggcaggct aaaggaaaga acgatgacgg cgttttagtt ttttctacgc cacaaatcat
     6421 ttcctgtgac tacggtgcag atagaaagcg cgcacgtttt gagattgggc gtgaacaagc
     6481 ggtcaaaaat gtgatctgga cggagttcgc ccacgccaaa ttaggcgatt atgttctgat
     6541 tggcgaaagc gagcaagccg atccgatttt ggctggcgca gaggaaatta ttcatataca
     6601 gcgctttgct gatacgttcg accgcaatcg tgatgatttt gcgctgataa caggcgggta
     6661 aaatggggat caaagtaaaa ggcataagac aggtcaagca gcggctgaat caggttgttg
     6721 gcgaggttca gagccaaaaa gcaactcggg caatgtttcg cattttgaat gcggttgccc
     6781 ctcttgcggc gcactatacg cctgttgata cctcaacgtt gatcaacagt caattcactg
     6841 aaatggaagt gaacggcaca cgcttgattg gaagagtggg ttattctgct aattatgcgg
     6901 cttacgttca cgatcctaat atcaagcaaa acttccgcaa gccttcagcg aagaaagagt
     6961 ttttgacttc cgcgctgaac gaatcaaagg ctacgatcaa atccattgtt aaagaggagt
     7021 tgtcactatg atttcttatg tcaaagcctt caaacaatgg ttagaggctc actctcttgc
     7081 agacggctat gttgtgcagc tttaccagtg ggaggatacg caaaaagcca agcctgttat
     7141 tgttattcag ccaaatagtg gttcgccgca agtttctgac ctgagcagcg aacactatct
     7201 gctaattagc cttgttgcag gtaaaaattc aggttacaca atcgaagagc gagcaagggc
     7261 gattatgtca aagattttag ttgagccttt cgcttccttt ggttacatcg aatcaatggg
     7321 cggattaccc gccccaattt tcaccgaaga taatcgaatg atttttcgct taccacttcg
     7381 gattatttca acaaatcaag aggagtagtt tatgggttct ccatctccac aaaataacgc
     7441 agaaaatctc gtggtggggc gtgccgtagt tttagagtac ggtacgactg atgcaaagcc
     7501 acaagaaagc gaatggaagg ttgcgggcgc aatgacgaca aagagttggg attttagccc
     7561 aaattcggtt acatcagagg ctgatgatgc tggcggcttc cctgaaacct taattacaaa
     7621 ctcagacttt agtatttctg gtgacggtga gtggcgcaaa cgaccaaaat cgaaagaact
     7681 aggcatcaaa gatctagttg tgttatacgc aaatgcggta aaaaatcgca cccaacctta
     7741 catttgggtt cgtttgaaat acgcaggctt aacaattctc ggaaaaatgg ttatcactgc
     7801 attaagtagc gaagccccaa ccaatgactt agtgaaattc tctatagagt tcaaggttgg
     7861 cgattcaagc actatcgaaa ttgctgcagc gtaaaaaatc ccaccctcta taacaagagc
     7921 ggtgggatta tcattatcct agggatagtt gtaggggctt tcctaagcta gccaaaatac
     7981 gaccaatagt gtcgattttt gtaatatggc gaggggctaa aattcgctgt acttctggcg
     8041 gacggatatt agttaatcgg gcaatttctg ccttgcttac cgcttgttct tgcattgtat
     8101 tatgcaaaag cacttttgcg tagatgctct ctggcataaa cacaggcact tcgtcggtat
     8161 ttactgggcg agtgagcgga aagcgtctat cttcatcgaa ataaatttca acacaagata
     8221 gtagcacgtc ttctgccatt tccattgctt cttcaaatgt atcgccttgg gtgatagctt
     8281 ctggcaaatc aggaaatgtc actacaaagc ccccttcttc agcgggggta aataacgctg
     8341 gataaaacat attttctcct tattcaaggg tgtagtgaaa gccccttaac atcaggggct
     8401 ttatattatt ttaagcctag ttgcttttta accccttcca ctaagcctgt ttttaactct
     8461 tggcttgggt gtttggggag gtggcttctt ttgcccttgt agtagagttt caaatgcttt
     8521 gatccatttt caacctctac cccattagcc tttagccacc ttaggaattc gctttgcttc
     8581 actacctcct cctatttgtt taacttgggt tcattatatg caaaaatgct aactttgtca
     8641 acagaaagtt agcaaaaatg ctaatttttt tgttgaggtg aaaatggtta gctatccaat
     8701 tactgaaatt ggtgaaatgc ttattgcaac cgatgggcga gactttttat tcagaccgag
     8761 tttcaaaaat atctacaaaa ttggaagccc acaagaaatt gtaaaaatct atgcactatt
     8821 gcacggctgt gagattcgag agatattgcc gcacatttta accaaatcgg agtctcatca
     8881 atcttacttt tggtcagtgc tgaattcgcc tgtttttggc agaaaaattc tgcaaaacgc
     8941 aatgatcgtg atgtcgtgct gttgcgatga tgatatttcc ctgttgattg gcgagtggtt
     9001 gccgtcaaag tctggcgtta ggtacaagtt gggtaaaatg ccacctgaga caattatcaa
     9061 ttttgcaaaa aatttaatgg gtcacggagt gattggttgc tgcgaattgc caaagaaaga
     9121 acaatctgca aaaaatgaat tttcagaaag ttttgatgct gttgagtaca taagtttagc
     9181 ccgaactcat ttcaatatga gccgcgaaga cgctgaaaat ctcacgatga ccgaattgca
     9241 gcaacttcta aaaagtcaaa tgcctgccga aaataatagc tatggacagt tcaccgaaga
     9301 agagtacgac agaattatgg cggaatatga gaaaagaaga gcggcttaaa tagccgctaa
     9361 catttaaaag aacctacctg atagaacggg agtgaaatcc ttgtaatgtg ctgaataaaa
     9421 aagctctctt atgtcattga gtagctcaaa ttcatctttg gatcttgcgt tgcagattgc
     9481 cacatagtca tctacgcagg ataaaatacg ttcaatactg acttctttta tccagcatac
     9541 ttcttggttt tcattcagcc agttggcaaa gtcttgttta gtgcagtctt ttttcataag
     9601 ttctcctttt tgattatttg caaaaaaaat catatcaaat catccgttgt tatctattgg
     9661 cgaaaataat ttttgcgaca cagatcgcaa aaataggttt tgacggtgga aaaacgaatc
     9721 ttaattcgta gaatggtaaa atttctttat ttttaaggta tttttatgaa aaaaatgatt
     9781 gtttttgtag cttctgtttt gtcatctttt gttattgcat cggattggga aataatagaa
     9841 gcggctcaaa ataaggtgaa agcagagctt ataagaaaat cagaaaaaaa agcagagtta
     9901 tgtttaaatg ttgctcaggc tgtcttaatt actaatcaaa aagtacaaaa agatactttc
     9961 tcaatgtgtg gtgaattttc gacagagatt gtcttttctg actctaaagc atatagcctt
    10021 gcagtatgtg gaaaggttaa aggtaaagat attatcggaa atccgataga gagcgactat
    10081 atataccaaa gagcaactaa ggatttttac tttaattata atggcaaaaa atcagcttat
    10141 tatgatcctc aatctgatct tgcaaggctt caagaggtct cagactctat gtttaatgct
    10201 atgcaaaata gatattgtaa gtgatgctt
//
LOCUS       DANLUA010000086        10148 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLUA010000086 DANLUA010000000
VERSION     DANLUA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..77
                     /locus_tag="PXB44_001542"
     tRNA            1..77
                     /locus_tag="PXB44_001542"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Met,seq:cat)
     gene            228..1688
                     /gene="xseA"
                     /locus_tag="PXB44_001543"
     CDS             228..1688
                     /gene="xseA"
                     /locus_tag="PXB44_001543"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL5282011.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            2000..2085
                     /locus_tag="PXB44_001544"
     tRNA            2000..2085
                     /locus_tag="PXB44_001544"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2034..2036,aa:Leu,seq:caa)
     gene            2183..3007
                     /locus_tag="PXB44_001545"
     CDS             2183..3007
                     /locus_tag="PXB44_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL5282012.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETISQLCALLMPQFANSHFSA"
     gene            3019..4380
                     /gene="dnaB"
                     /locus_tag="PXB44_001546"
     CDS             3019..4380
                     /gene="dnaB"
                     /locus_tag="PXB44_001546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5282013.1"
                     /translation="MSSLNKSLVQPHSIEAEFSVIGGLLLDNELFDEVSSKINEGDFY
                     NFTHRLLFKHISELIEAGKPADILTLDQYLTQKKVIEDLGGFAYLAEIVKNTPSTANI
                     HAYTDIVLLYSKQRQLLKLGQFIVDQTQSAKTPDKFEAVVDDIEKKITEFSLSDNTKS
                     AADLSDIFANMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLKKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADSLDDEE
                     WSKLTSAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSVEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEVYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            4389..6035
                     /locus_tag="PXB44_001547"
     CDS             4389..6035
                     /locus_tag="PXB44_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5282014.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLGKSLSSRELSSELENDGYIISHALILKMERCVNYLYPHIPEVLF
                     SGLGHTQIDKLLAIRNNAEEVWAAHQFDTDVTFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALEGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQT
                     AKSEPLIKNENKAVTPQKAQHIIDEVIDDNESDDEQNNAITDLSQLGSSLLDNQDNQE
                     DYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEAY
                     SLALDIAESMGIDHLVEHIVKQPVDYTYAMKPADTELSPSALTLYSFLSMLQTAEHKD
                     ENSCVLDSKFLFELDDLTLVKIFRLIRVSRHIRQQGGEND"
     gene            6028..6585
                     /locus_tag="PXB44_001548"
     CDS             6028..6585
                     /locus_tag="PXB44_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL5282015.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMEILDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            6744..7934
                     /locus_tag="PXB44_001549"
     CDS             6744..7934
                     /locus_tag="PXB44_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282016.1"
                     /translation="MFSSTNKPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLITD
                     KTKWHYVSHLDCLQSRYQIYQQQQLVKDKQPSQTEQELSKIQQNILTSTMEVRDFDEE
                     VRKKSSKNQTSNKGVRAKNEDKSLILDLLPLGKSDVQYRTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEVQQRIIQKQDVKKPKGYLYTLIQRAKQG
                     EFKPYYFELGKNLPSQNQANNNSAHSIQKLTEQPTEKPVLTSADREQMASRMQKFRAE
                     LLLQ"
     gene            8113..8871
                     /locus_tag="PXB44_001550"
     CDS             8113..8871
                     /locus_tag="PXB44_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL5282017.1"
                     /translation="MTTQLDPQLGPLRSEITFTLHTQYAHKLWVGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKSSGITRRDYQEKNARYQAAIKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNTTVEEEQSVETHITAQTNESQK"
     gene            8880..9365
                     /locus_tag="PXB44_001551"
     CDS             8880..9365
                     /locus_tag="PXB44_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL5282018.1"
                     /translation="MNLISDEIYRQLVKDSGLNTSLKQLFSHFDTGSDYELLQEQFTQ
                     ARAYFMAAQDSMVQSVRQDLSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDKSVPVEVRKKVVAVEKDRILINMQISVFNHILRQLADCAEKLKEVDNAVAKSDLN
                     S"
     gene            9721..>10148
                     /gene="ssb"
                     /locus_tag="PXB44_001552"
     CDS             9721..>10148
                     /gene="ssb"
                     /locus_tag="PXB44_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5282019.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRYAEICGQYLRKGSKIYIEGKLRTRKWQDQNGQERYTTEIQG
                     ELLQMLDSRNATNSVDGQQSNYQAMSQSPKATHPQDLPMD"
BASE COUNT         3298 a         2000 c         2043 g         2807 t
ORIGIN      
        1 cgcggggtgg agcagcttgg tagctcgtcg ggctcataac ccgaaggtcg ctggttcaaa
       61 tccggccccc gcaaccacat tccaaaccct cgatagtgaa tcacatcgag ggttttcgtt
      121 ttatttgcca aaaaacagca aaaaataacc gcttgttgac tgcaaaagct tcctctcgct
      181 ttcatttctc gttacacttc tactacattt acgctttttt gattattatg agcaatattt
      241 taactgttac ccaactcaac tattccgtcc gccatttgct cgaaatggaa atgagccata
      301 tttggctaac cggcgaaatc tcaaatttca gccaaccagt ttcggggcat tggtatctca
      361 ctttaaaaga tgacaaagca caagtgcgtt gtgcgatgtt taaaatgaaa aatgtaagag
      421 tgaattttcg cccacaaaac ggtatgcagg tgctggtgcg ggcaagtgtt acactctatg
      481 agccacgagg tgactatcag ctcattattg agagtatgca accggcgggc gaaggcttgt
      541 tacagcagca atttgagcaa cttaaagcaa aactttcagc tcaaggcttg tttgctcaag
      601 agcataaaaa gccaattcct acttttgtta aaaccgttgg tattattacc tcctcaagcg
      661 gagccgcatt acaagatatt ttacatattt tgcaacgacg agatccgagc ttatcagtgg
      721 ttatttaccc ggctctcgtg caaggcaaag aagcaacgca agatattgtg aatatgattg
      781 aactggcaaa ccgccgcaat gagtgtgatg tattaattgt cgggcgaggt ggcggttcat
      841 tagaggattt atggtgtttc aacgaagaga gtgtcgctta tgccatttat cattcacaaa
      901 tcccgattat cagtgcggtg ggacacgaaa ccgatgtgac cattgctgat tttgttgcag
      961 atttgcgcgc accaacgcct tcggctgctg cggaattagt cagcagagat caaaatgaat
     1021 tgaaacgcca attacaacat cagtttgata aagtgaattt agcctttgac cgagtttgga
     1081 accataaatt taaccgtttc caacaactcc ccttacgcct gaatgctcag catccaagca
     1141 aacaaattca atcgcaacag caacgttttg attatatgga ataccgcctc gagcaggcaa
     1201 tttatgcact tgttgcagcc aaaaagcagg tggggaaaga gctaaatgtc cgtttaattc
     1261 accaacatcc gcaaaagcag attgaacgcc aatcccaaat gcttaagcaa ttggagctga
     1321 aactccattg gcaaatggag cagcaactca gcaaaaaaca gcaaaaatgg aaaaacatta
     1381 cgcaacggtt tgaacgcaac ccgctacctt atcaacttta tcagcaacaa acgatggtgg
     1441 caaaaatgtc gcaacgcttg gcttattcta tcgaaaaaat aatgctgaaa gagcagcgac
     1501 attttcaaga gttatgcact aaacttgatg gacttagccc attgaaaatc ttgaccagag
     1561 gctactcaat tacccaaact aaagacggta aaacgcttac ttcaacagaa aatattgaac
     1621 gtggtgaggc tattatcacc caactaaaaa caggaaaaat aataagtcaa gtagtggatt
     1681 ttttgtaggg gaaattttga acgctgccaa tttttaaaat tatttatagt aaaatcagta
     1741 ggttaaatat ttataaacag ggttgttcac ttagttatcc acaggtgatg tgcataacta
     1801 tttttgtaaa gatgaattga gtgctgattt ttaaatcaac cgatttcaaa aggcattttt
     1861 acgcttgcac aagcggtcat atttccctat aatcgtgcaa aaattaagcc agtgtggcga
     1921 aatcggtaga cgcagcggat tcaaaatctt atgcgtaaac tgataggaca tacaggcgat
     1981 tgaataaaaa gcactcttag cctgagtgat gaaattggta gacatgacgg attcaaaatc
     2041 cgttggtgaa taaccgtgtc ggttcgagtc cgacctcagg caccatttga atcacacttg
     2101 tagtttctct ctttcaaata atcaattcaa atacaccgta ttaatggcca atactcctta
     2161 acccttgtta agagagtaaa ttatgaaacc attacaaaac ggtacaaaca aaccttttac
     2221 cattactatt gcttcaacta aaggtggctc tgccaagagt acgaatgctg ctaacattgg
     2281 tgcattctgt gcagaacacg gtctaagaac ccttcttatt gataccgata cccaacccac
     2341 attaagctct tattatgagt tggaatatca agctcccggt ggaacttacg aatttctgca
     2401 ttttaaagat gtagatccta cccacattat ttctagaacg actatcccaa atcttgactt
     2461 gatccagtct aatgacccgt caaataaaat cagcccaatg ctgagagatt cacctgacgg
     2521 tgcattacgt tttagtttac tgctccagaa aattgaaggt tacgatgtca tcattattga
     2581 cactcgtggt accagagata ttactgtcga tatgtctgta ttggcagcag atgttttatt
     2641 ctgtcctatt cttccccaca ttttatctgc taaggagttt attcgtggca cgattggaat
     2701 gtatcaagac ctcgaaacct ttacggcatt tggctttacc ctaccaccgc tgaaagcgat
     2761 gattaactgt gttgatcaca ctaaagatgt caaattggtt tccgactacc ttcatactct
     2821 cttccaaaat gagtttgatg aaaacaaaac attgctggaa ttttcggtac ctgaccgcgt
     2881 ggcatatcgt gaagcggcaa ctttttcaaa accggtctat cgtcagagcc aagctgaata
     2941 tgaaacgatc agtcagcttt gtgcgttact gatgccacag tttgcaaact ctcacttctc
     3001 agcatagagg gagaatgtat gtctagctta aataaatcac ttgtacagcc gcattctata
     3061 gaggctgaat tttcagtaat cggtggatta ctcttggata atgaactctt tgatgaagtg
     3121 agtagcaaaa tcaatgaggg ggatttttat aattttactc accgtctact gtttaagcat
     3181 atttccgaat tgattgaagc cggtaaacca gcagatatat tgacattgga tcaatacctt
     3241 acccagaaaa aagtgatcga ggatttgggc ggatttgctt atcttgcaga aatagtcaaa
     3301 aacactccat ctacagctaa tatccacgct tatacggata tcgttctctt atatagtaaa
     3361 cagcgacagc ttttaaaact ggggcagttt attgttgatc agacacagag tgcgaaaacg
     3421 ccagataagt ttgaagctgt tgttgatgac attgagaaaa aaattactga gtttagccta
     3481 tcagataata caaaatctgc agcagattta agtgatattt ttgccaatat gctctctcgt
     3541 atggaactct cggctaaaaa tggagatcct gtgacaggta ctccaactgg tattcaaggt
     3601 attgacgaag cgacaacagg tggacagcca ggcgatttaa tcgtgattgg tgcccgccct
     3661 tctatgggaa aaaccgcatt ttctcaaact atcgcttacc atacacttaa aaaattcgag
     3721 tcggctcctg tgttctatca cagcatggaa atgccggcag atcagatttt acaacgtttt
     3781 ctagctatgc gtgccagagt gggattgcaa aatattagac aggcagacag tttggatgat
     3841 gaggaatggt caaaactaac ctcagcaatg ggctatatcc tagaacattg gaaaaatcgt
     3901 ttagttattg atgatgaagg tagtctcacg cctcaaaaat tacgcactaa agttcgacaa
     3961 aatgctcgta aatatggcaa accggcagct attttcattg attacattca gttaatgcaa
     4021 acctcgcaac gttatgaaaa tcgcaattta gaaattgcca acatctctcg ttcactcaaa
     4081 ttactggcaa aagagatgga ttgtccggtt tatgccctct ctcagctgaa ccggtctgtg
     4141 gaacaacgtg ccaataaacg cccgatcaac tctgatttac gggaatccgg ctcacttgag
     4201 caagatgccg atgtcatttt attcatctac cgagatgaag tttatgatgc ggattctccg
     4261 tttaaaggca ctgctgaaat cattatcggc aagcaaagaa acggaccact tacttccgta
     4321 atgacgaatt tcagaagtga atttgcccta tttgaaaaca gaacaaacac aacgcattag
     4381 gatagattat gacaaaagat gaaaaaagac tggcagcgat agcaaaagga ttaaatgttt
     4441 cacctatttc aaatacggct ccatcctacc aaacagccac accaacgcat tacagtagcg
     4501 atgttgaata tattacagta acgctggata agttgcgtcc gtatgagcat aaccctcgga
     4561 aaacccgtaa ccctaacttt gagatgatca aagagtctat tcgtcgtcga gggttagatc
     4621 ataagccgaa tatcacccgt cgtccgggag aagatttcta tattatcgct gacggtggta
     4681 atactcgtat tcaggcactc aaggagctct ttaccgagac caaagatcca aaattttggt
     4741 ccatcagttg tcaatataaa ccttggcaag gtgatactgc cgacagtgtc gaagctgaac
     4801 tcaatttact tatcgggcat ttgattgaaa atgatacccg tgcggatttg tcatttattg
     4861 aaaaagcatt ggggattttg caggcgaagg agtattacga gaaaaaatta ggtaagtctc
     4921 tttcttcgag agagttatct tctgagttag aaaatgatgg atatattatc tctcatgctc
     4981 tcattcttaa aatggaacgt tgtgttaatt acttatatcc acatatacca gaagttcttt
     5041 ttagtggtct tggtcataca cagatcgata aactattagc tattcgtaat aatgctgaag
     5101 aggtatgggc agcccatcaa tttgatactg atgttacttt tgaatcgctc tggtcagaaa
     5161 atttatccaa atttaatgag gctacaccat ttcaagcaaa agaattccaa agtgaattga
     5221 ttacggcaat ggttgaagct ttagagggta aagtgagttt tgaaacactt tatctggata
     5281 ttgacttaga tgaacaaaaa ttcaaaaaaa tagcggctaa acaacaagaa attgataatt
     5341 tagttgaaaa aacagtcgat caaatcaata ccagacagga acaacaaact gcaaaatctg
     5401 aacctctcat caaaaatgaa aataaagccg ttactcccca aaaagctcaa catattattg
     5461 atgaggtaat tgatgataac gaaagtgatg atgagcagaa taacgctatt actgatttat
     5521 ctcagcttgg ctcttcattg ctcgataacc aagataatca agaagattat ctatcccaaa
     5581 ttgcccataa ctttggactc actccgggaa tgagtattca agaacagcga gagcaacgag
     5641 ctgaagcgaa tggactgact tttgcttgtg aaggtcgcca accggttgaa gatatttggt
     5701 tgattcaccc cgcacgtcaa tatcgctcag aggcctactc actggcctta gatattgccg
     5761 aaagtatggg cattgaccat ctggttgaac atattgtgaa acaaccagtg gattacactt
     5821 atgcaatgaa accggcagat actgaacttt caccgtctgc tttaacactc tactcattcc
     5881 tctcaatgtt acagactgct gaacataaag atgaaaatag ctgtgtctta gattccaaat
     5941 tcctgtttga gcttgatgat cttaccttag tcaaaatttt ccgtctcatt cgggtcagtc
     6001 gccatattcg ccaacaagga ggagaaaatg attaagccga ttaccgtaaa tgaagcattg
     6061 attggtaacg ccctgctcaa tattaaagag ggaaatatcc atccttgtct aaaaatggga
     6121 ttttctgagg agcaactaaa agcgatcaat aatctcagta ttgatgaaat tatggatatt
     6181 agcaactccg ttgtctcttt tgctaaggtg gagattaatc acgaaacctt ttggaaatta
     6241 ctggctattg cacaagccaa tactcaacag cgacaaatta ttgaccgagc attattgctc
     6301 ggagcctcta ttgaaatgtt gcaccagtat tttggtttat caacctctga agtctcggct
     6361 cgccgtcaat tactcggtat cgaagaaaaa atgggacgaa aagcggcagc aagtgatgaa
     6421 gaatctaccc atatttggga aatttggcaa agatacaaac aacgaatgga aatcctagat
     6481 tcacaagaag gattagagct tctatgtctg attgctgagg aaggcaatat gaatctaacg
     6541 gttgtttgga aactggtttc cgaatggtat tcaaaacata agtaaaaaat aaatgccccg
     6601 aagttggagc tttggggcat tcgatattga gtcttgtatc tctatcttat gaattacaag
     6661 gactttgcag aagtcccgtg cattatagcc ataggagaga gaatagcaag agaaaaactc
     6721 ttttatcctg gtggagagaa acaatgttta gcagcacgaa taagccaaca gagcatggat
     6781 tgctattttt tggcaaccaa cacgaaacag tccctactcg attattacaa gatccctgcc
     6841 ttacaccaag agcaaagttt gcttggcagc tcattaagtg caatgttcaa gcctttcaag
     6901 gaggattatt tccctcttat gagacgctca gcaaaatgtt atccgataga ccttatgcgg
     6961 aggctaaatt atcggaaaaa gtcgtgactc aaacactcta tttactccgt cttactcgct
     7021 ggcttacttt atgtgaaaca gtgcgtgatg agcgaggtta tgtacaaggt aatgtttata
     7081 tcctacatga tgaacctgtg ccaattttag atgcaattca attaaatagc gactatctct
     7141 cttttttaga taaatgcacc aaacataaag atagccttat taatggtgtg gcaaatcata
     7201 ttgttgaaag tctaataaca gacaaaacca aatggcacta tgtctctcat ttagactgtt
     7261 tacaaagtcg ttatcagata taccagcaac agcagctggt gaaagataaa cagccatcgc
     7321 aaacagaaca agaattaagc aaaattcaac aaaacatcct aacttccact atggaagtta
     7381 gggattttga tgaggaagtc aggaaaaaat cctctaaaaa ccagacttcc aataagggag
     7441 ttagggctaa aaatgaagat aagtcattga ttttagactt acttccatta gggaagtcag
     7501 atgtacagta ccgtacttta tatattaata agtactgtac tggggaaccg agcgaaatta
     7561 cttggccaca agaaatccca ttaacttcac ttgaacaaaa acttactgca caggcaatga
     7621 agggattaga cctcggttta tgccaagcta ttttgttgga agtacaacaa cgcattattc
     7681 aaaagcagga tgtgaaaaaa ccgaaaggtt atctctatac gcttatccag cgggcgaagc
     7741 agggtgaatt taaaccctac tattttgaat taggaaaaaa tcttccctca cagaatcagg
     7801 ctaacaataa ttctgctcat tctatacaaa aactgactga gcagcctact gagaaaccgg
     7861 tcttaacttc agcagacaga gagcaaatgg ctagtcgtat gcagaagttt agagctgaat
     7921 tacttttaca ataagattaa aaaacaatca ctgttaaggg gcttaatagt gagtgaaaaa
     7981 taatcagtgt gtaccaggta cacaatggat aaaaaataaa cagcgtaggc agtgtctgca
     8041 aagattaaaa aacagacaat ggaaagcccc tttccaaaga ctaaaaaata atcaaataaa
     8101 caaggagcat atatgaccac tcaattagat cctcaacttg gtccacttcg tagtgagatc
     8161 acttttacgt tacataccca atatgcccat aaattatggg tggggcgacc aatgattcgt
     8221 aatgaggaag gcaaagtaac tcaatcctcc attatcagcg taccaaattg ctttgcaatg
     8281 ctaacccaaa ttcaacgggc tgcatcagaa gatgatcctt atgctgacga ttatctgatt
     8341 caatttgaag aaagtgtgat caattatcgg aaggaaatcc aaaagctcac caacgacatt
     8401 gtcacgctct acgccaaaat gttgccggag ggaatttcta ttgaacgctg tgccaatatt
     8461 tccccgattt cttacccgat ttatgttaat tcacagctag ggtatcaatt actctactta
     8521 cttggtgatt ttgatagcct ttgccgttca gtaatgacgg cagctcatat tgcgattatc
     8581 aatcgtgcag aagctcaaga ttggattgaa gctggtgcaa ggttaatccg gaaatgtttt
     8641 gggatcgttg agcgttataa gagttcagga atcactcgtc gggactatca agaaaagaat
     8701 gctcgctatc aagctgcgat aaaacgaatg ggctatacac tctcagatgc tgtacttacc
     8761 ggtgagcatc gtgctgagtt tgctcccttc atcaagcaaa acactactgt tgaagaagag
     8821 caatccgtag aaacccatat cacggcacag acaaatgaat ctcaaaaata aggagtgata
     8881 tgaacctcat ctctgatgaa atttatcggc aattagtcaa agattctggg ctgaatactt
     8941 ctctgaaaca gctatttagc cattttgaca ctggctcaga ctatgaactt ttgcaagaac
     9001 agttcactca agctcgggct tattttatgg ctgcacagga tagtatggtg cagtctgtgc
     9061 ggcaggatct ttcacctttg gctgtttata tgattaagga taaggcctca tcctcaggcg
     9121 gtacattttt acgctggcgt agtatgcaaa atgccagaac cggtggcaca gtttggcagc
     9181 cgattgtaga tgataagtct gtcccagtgg aggtgaggaa aaaagttgtc gcagtagaaa
     9241 aagacaggat tttgattaat atgcagattt cggtgtttaa tcatattttg cgacagttgg
     9301 cagattgtgc tgaaaagttg aaagaggtag ataatgccgt tgcgaaatca gacttaaatt
     9361 cgtagtaaat ttatatataa caactcctat attggttgtg tgtaaacgcc aaccacggtg
     9421 ggctgacgtg gtaaaacaaa cagtccgtta attaggacat attcaagttt gtaatacctg
     9481 aaaatccggt ttgtaatccc tgaaaaagtg cagtttgtaa atcctgcatt aaaaggcctt
     9541 ttaaaggtaa gggtacaagc cccttacctt cacgaagaaa aagcctttac aacggaggaa
     9601 taagtgattg cacacgtaaa aatgaaaaat tcactctaac caaattttaa ttaaatactt
     9661 ttttcttagg ctgaatcaag tttaatagaa ctaacttaat ttattgaagg agtaaatacg
     9721 atggcaggaa tcaataaagt gattattgtc ggtaatcttg gcaacgatcc tgaaatgcgg
     9781 actatgccta atggtgaagc agttgctaat attagtgtgg caacttctga aagttggacg
     9841 gataaaaata ccggagaacg tcgagaagtc actgaatggc atcgtatcgt tttttatcgc
     9901 cgttatgctg aaatttgtgg tcagtaccta cgtaaaggct ctaaaattta tatcgaagga
     9961 aaattacgga cacgtaagtg gcaagaccaa aacgggcaag agcgttatac aaccgaaatc
    10021 caaggtgaac tattacaaat gttagatagc cgtaatgcta caaatagcgt agatggacaa
    10081 caatctaact atcaagctat gagccaatct ccaaaagcaa cacatccaca agatttacca
    10141 atggataa
//
LOCUS       DANLUA010000087        10023 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLUA010000087 DANLUA010000000
VERSION     DANLUA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..373
                     /locus_tag="PXB44_001553"
     CDS             <1..373
                     /locus_tag="PXB44_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282020.1"
                     /translation="FALVQAELKYSGSNIPSEYSKFNLENVLIDFPNKTNSVAYQNLV
                     IHNAKTRAGKLKTQYANAHDLGNLVLVEDIQKIFSGLLEIIYQVRCHLVHGSLSPTPE
                     NHEVVKYCYLILWDCLKGFCD"
     gene            complement(641..1378)
                     /locus_tag="PXB44_001554"
     CDS             complement(641..1378)
                     /locus_tag="PXB44_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282021.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(1375..2343)
                     /locus_tag="PXB44_001555"
     CDS             complement(1375..2343)
                     /locus_tag="PXB44_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5282022.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            2524..2859
                     /locus_tag="PXB44_001556"
     CDS             2524..2859
                     /locus_tag="PXB44_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282023.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            2843..3166
                     /locus_tag="PXB44_001557"
     CDS             2843..3166
                     /locus_tag="PXB44_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5282024.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3156..5042)
                     /locus_tag="PXB44_001558"
     CDS             complement(3156..5042)
                     /locus_tag="PXB44_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5282025.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5072..5872
                     /gene="metF"
                     /locus_tag="PXB44_001559"
     CDS             5072..5872
                     /gene="metF"
                     /locus_tag="PXB44_001559"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL5282026.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(5921..7081)
                     /locus_tag="PXB44_001560"
     CDS             complement(5921..7081)
                     /locus_tag="PXB44_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282027.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7141..8157)
                     /gene="galE"
                     /locus_tag="PXB44_001561"
     CDS             complement(7141..8157)
                     /gene="galE"
                     /locus_tag="PXB44_001561"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL5282028.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8245..8406)
                     /locus_tag="PXB44_001562"
     CDS             complement(8245..8406)
                     /locus_tag="PXB44_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL5282029.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(8384..9385)
                     /gene="lpxK"
                     /locus_tag="PXB44_001563"
     CDS             complement(8384..9385)
                     /gene="lpxK"
                     /locus_tag="PXB44_001563"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL5282030.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(9387..9791)
                     /locus_tag="PXB44_001564"
     CDS             complement(9387..9791)
                     /locus_tag="PXB44_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5282031.1"
                     /translation="MKVHEKIKLLRENNHWTQDEMAQKLSMTTKGYAKIERGETISNL
                     PKIEQIAEVFNMDICELLSYGEEGKIYINNTDNNLTNSFISLGNGVEEIQRLQLIISH
                     KDELLAHKEEIIENQKRELALLRDLVETLKNK"
BASE COUNT         2761 a         2140 c         2195 g         2927 t
ORIGIN      
        1 ttttgcatta gttcaggcag agttaaaata ttcaggtagc aatattccaa gtgaatattc
       61 taaatttaac ttggaaaatg tgcttattga ttttcctaac aagacaaatt ctgtagcata
      121 tcagaatcta gttattcaca acgcaaaaac gcgtgcagga aaattaaaaa cacaatatgc
      181 aaatgctcac gatttaggca atttggtttt agtagaggat atccaaaaaa tattttccgg
      241 gttattagaa ataatttatc aagttcgttg ccatttggta catggtagct taagtccaac
      301 acctgaaaac cacgaagttg ttaaatattg ctatttgatt ttgtgggatt gtttaaaagg
      361 attttgtgat tagttattgt caataaagct acaagcggtc aattctccca aaactttgca
      421 aatttccccg caaaactgac cgcttgttct cctctatcca ttcacctcaa ccaacttttc
      481 tctctacccc tactcaaaat cggttaaaca atctgttgag gtaaatgttg tgtcggaatg
      541 ggatttaatc ttaatgaaac tgaaaaaatc ttctgccaag caaatttccc taaaattgga
      601 agttcatttc caatttctca aaaaatcacc caaaccggaa ctacacttcc aatttttcaa
      661 tcccccacat ttcccttttc tcccaatcca tttcagcttt caccccaaaa gccgttgcaa
      721 tattttcggc atttaagacc gcttgtgtgt tgccgctgac gagtaaattg ccgtttttaa
      781 gcagaatgat ttcatcacaa aaacggtagg caaggggcaa atgatgaatc accaccacgc
      841 aggtttgtgt tggagttagg gctttgagct gctgcataat atcgatttgg tagtaggggt
      901 cgagggcggc aatcggttca tcggcaagca agatcggggc ttgtttcatc aaacaacggg
      961 cgagttgcac tcgggtctgt tcaccgccgg agagctgttg gtaagtgttg tccagcaggt
     1021 gagaaatcgc caattgttcg gcaaccgaac gcaccttttg acgctcttgt tcaggcggaa
     1081 gcggtgcggg caagccgagt gcaatcacat catacaccga aagttgccac gccacgttga
     1141 gctgttgggc gaggtaggca aattgtcggc tcctctcaac ggcggaaagg gcgtgcagtt
     1201 tgcaattttc cagccaaatt tcaccgcttg tcgggcgtag aatgccggca atcgctttga
     1261 gcaacgagga tttccctgcc ccgtttgcgc ccataatgcc gatcaatttg ccttttggca
     1321 gatagcaaga gaggtgttga ataccgtggg caagggtgag attttcaatc ttgatcattg
     1381 tgccaatctc cattgctggg tgaataaaat ccaaatcaaa aacggtacgc ccaaaattgc
     1441 cgtgagcgtg ccgatgtaaa ttcgggcaaa aaacggcaca tattgcaccg ccagatctgc
     1501 aatcagcagt aacaacgccc cgattaaagc ggaggtaaga taaagctggg acggcggttt
     1561 tttcagccaa atacgggcaa gatgaggggc tatcaagccg ataaaaccga tggtgccggt
     1621 ctgtggaatc gttgctccga ccaacagcgc cacgccaatg ctaacggtaa aaaaactgcg
     1681 ttgcaggttg atgcccattg ttgctgccgt ctcttcgcca aaggtgagca ggtcgagata
     1741 acgccgttcc cgatagaggc agacaatgcc gagcaagatc aacggtaacg cccaacatac
     1801 tgcctctgcg gttgcccaga ctaatgagcc ttgcaaccag cggtagagtt ccgccaacgc
     1861 ccacgggctt tcggcattgg aaagcagtaa agtcaccgct gccccgagca acacgtttac
     1921 cgccacgcca cttaaaatca tcattgttgt gccgtggcga aagctaatca gccacaccag
     1981 caaaaatgcc cccagcgccc ctgccacacc gccaaacagt agccagccca tcggggctga
     2041 aaaatagtag agcagaaata cgctcaccgc cgttgccccc gaactgctcc caagcaaacc
     2101 ggggctggca agcggatttt taaacacgcc ctgcatcgca ttgccggcaa gcgcaaggct
     2161 ggcgcccgtc agcaccgcca agccaatacg gggcagtcga atttcaccca gcaccagcca
     2221 gcgcagatcg cttagcacgc cttccgcatt tttaagcaag gcaaaatcgc tcagttggta
     2281 aaaggctgcg ttgctaacaa tcagcaacaa caagcttcca agcacaatgt ttaatttctt
     2341 cattcttctt tctctcatca aacgtgcaaa ttataccata gcgccaaagt caagctgaaa
     2401 acgctatttt tgcgatcatc atcgaaaaac tagcgtttgc cgaatgtaac ccacgacagg
     2461 ctttgctata ttccttcaat cagcctaagt tttgtattta aaacattaca gattgggggt
     2521 agaatgtacc atattgaacg cactgaaatt ttcgaccaat ggcttgaaaa tttaaaagat
     2581 ccacttgctg tggtgaccat tgcaagacgt attgaacgtg ccgaaagcgg aaattttggc
     2641 gataccaaat atatcgcaga aggaatttcc gaaatgaggc taaacgtcag caaaggttat
     2701 cgcctttact atgcgcaaaa aggtagcatt atttatatac tactaaatgg cggacataaa
     2761 ggcacgcaac aagctgatat tgataaagct aaaatgctat gggcagaaat taaacaacat
     2821 atggcagaac aggagaaaaa gaatgaacga attatatgac gccccaattc cacaagctac
     2881 tcgtgaaaaa tttggcattc aacctttcga tgccgctgaa tacctcgcca atgacgagct
     2941 aatcgccctt tatttggcgg aaacattgaa agacggcacg gacgaggagt ttattcaagc
     3001 attgaatacc gtggcacgag cgaaaggaat gaatgagtta gcgaaaaaag cagggatcgg
     3061 gcgtgaaagt ttgtatcaaa gcctttccag cagtaagccc cgttttgaga ctatccgcaa
     3121 aattattagc gcattaaatt tggagttgtc ggtcgttaag gcgtaagttt gtgcattaat
     3181 tgtatcccct gccaaacccc gtggtcgaaa cagtaggtgt atttcatcgg taggacaagc
     3241 ggtggatttt ggctcatttt ttgccatatt ttttgtaatg ctgggtgttt gagcagttcg
     3301 gcttggttgt tatagctttg gttatcggtg aggaacaata actgtttggg ttgcgctaac
     3361 agcagttttt ctagcgagaa attctgtggc gtgaggtcgc tttttaatgg tgacagaccg
     3421 agaagatcca acaacgtttg atattgcgga aaatgcggtt cgatcatacc cgtttcgctt
     3481 agaattaaag tttcagcaag cggttgtttt agcggaattt tttgcaaacg tagccgttcc
     3541 accagctttt ccgctttggc ttgattttgg gttagctcgc ccagttgtaa aatcagtgtg
     3601 aaaagctgtt cgggtgtttg cggactgtcg tttaatgcga ctaacttaaa gccaagctgt
     3661 ttcagccgtg cggtgagttg cggatagaag gtttcattca gcaaaatggt gctgtcggca
     3721 tagggcaaca gggcggtgag ttcaggctca acggtgggct tgtccgtgtt cagtttatcc
     3781 aacatcatca acggcttttg ggaatagggc gacatcgccc gaatttgctg tggttcggca
     3841 agttccagca acagacggtc gctacacaaa tttggcgata aacggtaagc tcctgaaata
     3901 cgcccagtga tagaattttt aaactgtgaa ttatcggtgt aacggccact aatagaaaaa
     3961 ctgtgatctt gttcggtaaa taagcgatat tctagtgccg cacttttttc ggataaggtt
     4021 ttttcacgca aatatttatc agaggtatag cgtgtttttg cataatctgt cagtaaactg
     4081 accgcttgtg tcacttctcc ttcacggtca aagtttacat ctaattgata attcgcatta
     4141 agttgttttt cgttgctgta gcgagcattt gaaccgaaat gtgttgcttt actattgaat
     4201 tggctgatat ttgcttggtg tttcagcagc tcttgttcat ttccccaata accgcctaat
     4261 ttaaaagttt gattgcgagt acgggaatat tcaccgctaa cgtctgaatt atagccatca
     4321 atatgaacgg tttgggaaga ttgagcggca agaacttcta cccctttgtt accgaggtca
     4381 taacctaaac gtaaagagcc gttatcacga tgaaagccat ctcgttcgct tgcaccaccg
     4441 gttttcactt ctgagcctgt ttctgtggta tagctgaaat ggtttttact cagtgcggaa
     4501 atgcctttgg tacgatggct atcaccgtgt aaggcatagt agaaaccgtt gtgaaatccg
     4561 gaaagtgttg cgctggcatc acgggtatta tttgagccta aaccaagatc gacctccgca
     4621 ttgaatggtt tctctttgta taaaccgctt ttagtggtga tataaactac accacccata
     4681 gcactgcttc cccaaagggc ggattgctcg ccacgcaaca cttcgatacg ttcgatattg
     4741 ctaatcggta agccaccaaa atcgaaattt ccaatattaa tcggattgac ttttacaccg
     4801 tcaatcacaa ccgcagtatg gcgagattct gcccctcgta aaaataagct agtaagagta
     4861 ccacgcccac cttgttgccc aatcgccacg cccggcacag tctttaacac atcactcaca
     4921 taagtcgcat tgcgtgcggc aaaatctttt tcggtaagaa ccgtcacgct ggaagcggtt
     4981 ttgtcttggt tgattggggt tgcataggcg gaatagactg ttacggaatc aagctgagtc
     5041 atctcatttt gtgcaaaagc tgtacttgct agtgcaacag aaatggcgaa tgctgtggga
     5101 ttctattgat cgcctaagtc ctttaaaacc gaaatttgtt tcggtaactt atggagcaaa
     5161 ttctggtgag cgagatcgta cacacagtat tgtgaaatca atccaagaaa aaacagggat
     5221 catttcagct ccgcacttaa caggcattga tgcaacaccg gaagaattaa aggtaattgc
     5281 caaagattat tgggatagtg gtattcgccg aattgttgct ttgcgtggcg atataccgaa
     5341 aggttatgat aaagcactat tttacgcctc agatttagtc gctttattaa aatcagtagc
     5401 agatttcgat atttccgtgg ctgcctaccc tgaagttcac cctgaagcaa aatcagcaca
     5461 ggcagattta cttaacttga aacgtaaaat cgatgcgggt gcaaaccacg ttattacgca
     5521 attctttttt gatgtagaga gttatttacg ttttcgtgat cgctgtgttt ctattgggat
     5581 tgattgtgaa attgtgccgg gtattctgcc ggtgacaaat ttcaagcagt tgcaaaaaat
     5641 ggcagcagtg actaatgtta agatcccgac ttggatgggt aaaatgtacg aagggttaga
     5701 tgatgatcaa accaccagaa acttagttgc agcaagtatt gcaatggata tggtgaaagt
     5761 gctttctcgt gaaggggtga atgatttcca cttctatacc ttaaatcgta gtgaactcac
     5821 ttatgcaatg tgccatactt taggcgtaag acctgattta acaactgcat aatcgaacat
     5881 taaggctgta tagctaaatg agctatacct ccttatagtt ttacttctct tcagccaccc
     5941 aaatctcgcc attttttaat aataaatagg ctttgttgga atcggctttg gtttgtttaa
     6001 ttccagccgg cagggcggtt ggcaattcag tgatcttgcc tgttttatcc aacacaacaa
     6061 cttgttgata gcccatttgg ggtatcagtg cgaaagtctc tgttgtcgcc actaaattgg
     6121 tttcgtgatc accatagcgg tgattgctta agccggaggt aataaaacgt tcagctaatt
     6181 ggtttccttt caggctgtat tcaaccaaat tgccttttag atgtggcatt tggattgcat
     6241 agagcttgcg gttaaagata aacggcgatt gccaacggtt agtcgacagc ggctcacttt
     6301 cggcttctat tgataatttt ccgtctttaa cgccaatcac aacagtgctg gccccattgc
     6361 catcgccaga gataacagaa acgatcttgg tttttccatt ttctgtgaaa gtcgcccagc
     6421 ggttggcttc aaatactcgc ccttgttggg tgagtttttc tgctaagggg tttagggtat
     6481 ggcgttctag aaattgaatt tcgcctgcct ctacatcatc gcctaataca ccgtgttgat
     6541 atgtaaaatc atcaggcttt gcaagtacag caatatgccc ttggttaggt gtttcggcat
     6601 caaaatcaat ttgcagaggg tgagcatcag gcaacacctc ggtttctgcg gtggcagtaa
     6661 tagtggcttg gtttccttgc gtttcaattc tggctaactt aaattgtccg ttttgtttgg
     6721 tcacggcaat ggtggcaaaa ttaagggcgg tcattgtagc tttcggggca aggggaatgt
     6781 tggaggtata gattttatca gccgtccaca gcataaaatg cccgttttga tccgctaacg
     6841 caattcgttg gtattgagct ttaggttcaa tctcggtgga aactccatct gccagttttt
     6901 gagcttgttg tgtgactctc cataattgtt tttgttggtt aattaccact aattcgcctt
     6961 gttctgaaag gtcaaattgg cttactttgc caatttgggg aagtgggagt ggagaaagct
     7021 gaacatcagc aaacaaagaa gcggagaaca agcttaagat taacagaata gattttttca
     7081 ttagcgttac ctaaaaagaa gatttaaatg ataaagggag atgttatctc cctttacgct
     7141 ttagccttta taaccattcg ggttattttt ttgccaattc caagtgtctt tcatcatttg
     7201 ttctagccca cgctcggttt cccaacccag ttgctctaac gctttttgtg gagcggagta
     7261 gcaaacggca atatcgcccg ggcggcgatc taccactttg tatggaatag taatgccatt
     7321 tgcggcttca aaggctttta ccatatctag caccgaataa cctgttcctg tgcctaaatt
     7381 gtaaacgtgg aaaccggcat cattttggtg cttgtctaag gcttttaagt gaccaagtgc
     7441 taaatctacc acgtggatat aatcacgcac acctgtgcca tcgtgagtat tataatcgcc
     7501 accaaacacc gataattgag gtaatttgcc cacagccact tggctgataa acggcattaa
     7561 gttatttggg ataccgtttg gatcttcacc gattaaaccg ctttcgtgag cccctaccgg
     7621 gttgaagtaa cgcaatacta ccgcacttaa ttgtgggaag gctttaacgg tatcttcaag
     7681 aatgcgttca accatatatt tggacgtacc gtaagggttg gttgtgccac cgacagggca
     7741 agattccaca atcgggataa tctgcggatc accataaacg gtcgccgatg aactaaacac
     7801 aatggtattc acattggctt ttagcatctc ttctaccaat acaatcgagc cggttacatt
     7861 gttttgatag taacgtagcg gttctctgac gctttcgcct acagctttta aaccggcaaa
     7921 gtgaattacc gattcaatct gattttccgc aaaaattttg cgtaaaatat cacgatctaa
     7981 aatatcgcct tgatagaatt ttacgctctt gccggtgatc tgcttcacac gctcaagtga
     8041 tacttcagag gaattagaaa gattatctaa caccacgatt tcacgatttt cgtttaataa
     8101 ttcaactaat gtatgtgaac cgatgtagcc tgcaccacct gtaactaaaa ttgccatttt
     8161 atgttcctct ataaaattaa tacgaaccaa ggttattgtt gctattttac ccgatttaat
     8221 caggcttgct aatatttatt cagtttataa tgccactccg gcttccggca gtaattccgg
     8281 aatgccgttt ttaatcgggt aagcaatatt gagcttaggg ttaattaatc ggttgttctc
     8341 tttgtcccat tctaattttt cattagagac agggcagacg aggttattga gtagtgtttc
     8401 gttcatcttc ttttcctagt ttttgtagaa tcggggctaa caaacaagcg gtcgattttt
     8461 ctgaaaatgt tgcagaaacc ggtacatacc accaatttgg ctgtgcaaaa tcgtggcatt
     8521 ttaccgcatc tttctcggtc attaataacg gcgtttggct atcagcaaat tgagccagct
     8581 gagccttatc aaaggcttga tgatcggcaa agccttgagt tttggcaagc ggaatgccta
     8641 atttttctaa catcgtaaaa aagcgtggtg ggtgaccgat accggcaatc gccacacagg
     8701 gttgggtaaa ttcgcttaat ggcttgcgtt gattggtgac taaattaatt gcatcagacg
     8761 gttcaagcgt cattaaatgc tcgttagctt gcggtgtgcc gccgttgcaa ataaccgctt
     8821 gcacggagtt taaacggctc ggtaattcac gcaatccgcc cgccggcagc acaaaaccgt
     8881 taccgaaacg ccttacgcca tcaaccacca cccattccat atctcgctgt agggcatagt
     8941 gttgcaagcc atcatcggtg atgatgatgt ccggctcaaa ctgatttaat agtaattcaa
     9001 tgctttgttg gcggtttgga gaaatagcca aaggcacatt ggttcgctga acaatcagaa
     9061 ccggttcatc acccaccatt tcagcagaac tttctcgggt aactaattgc ggaaacgttt
     9121 tcgctttacc gccatagcct cgagaaatca cgcctacttt tacgccacgc tgttgcagtt
     9181 gttccaccag ccaaacgacc actggcgttt tgccgttgcc tccaaccgag atattaccga
     9241 ctaccagcat aggcaaaggc gaacggtacg attgcaaaat attcagagaa tataaccgct
     9301 tgcgagcttg actaatcagc caaaacagca acgaaagcgg cgaaagcagc caagtgataa
     9361 gggattttgt ttgccagatt ttcatattat ttatttttaa gtgtttcaac taaatctcgt
     9421 aataaggcta attcacgttt ttggttttcg ataatttctt ctttatgggc taataattca
     9481 tctttgtggg agataataag ttgtaaacgt tgaatttctt ctactccatt tcctaatgag
     9541 ataaatgaat tagtgagatt attatctgta ttatttatgt aaatttttcc ttcttcccca
     9601 taggataata attcgcaaat atccatatta aatacttctg cgatttgttc tattttcggt
     9661 aaattagaga tagtttcacc acgttctatt tttgcataac ctttggttgt catacttaat
     9721 ttttgagcca tttcatcttg tgtccaatga ttattttctc gcagtaattt gattttttcg
     9781 tgtactttca tataacacct atagattgtg ggagttggga tataacttga tgtggtaaag
     9841 gataaatttt gtggattgta gcagtttatt gagcaattta gaatagagta aattttatct
     9901 taagctcaca ggagaagaaa cattcatgaa taatcaatat aatccattat atggaatgct
     9961 aggtatttta gcgtcagctg catcagtaat tttgcctctt tatgcagcat ataaagacta
    10021 tca
//
LOCUS       DANLUA010000088         9945 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLUA010000088 DANLUA010000000
VERSION     DANLUA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            103..1995
                     /gene="mnmG"
                     /locus_tag="PXB44_001565"
     CDS             103..1995
                     /gene="mnmG"
                     /locus_tag="PXB44_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL5282032.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQVAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2090..2314
                     /locus_tag="PXB44_001566"
     CDS             2090..2314
                     /locus_tag="PXB44_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282033.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2327..2941
                     /gene="rsmG"
                     /locus_tag="PXB44_001567"
     CDS             2327..2941
                     /gene="rsmG"
                     /locus_tag="PXB44_001567"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL5282034.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3209..4657
                     /locus_tag="PXB44_001568"
     CDS             3209..4657
                     /locus_tag="PXB44_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5282035.1"
                     /translation="MNRVTNKADEFAKLSTFERIAYGSGDLAQNLVFGTVGGFLLTYL
                     TTVNGISAVAGATIFLIVKWGNVFWDPFVGAFIDKSKPSAQGKYRPYLLKFGIPLVVL
                     ASLLFLPLESVRGSVIYAFLSYFATAMVYSFVNIPYGSLSASLTRDTDEIAKLTTTRM
                     TMANIANLFVYTLFPLFVQLLAPHAELKNTGFFGIELYLGDYMSAAAGPAWFKVYAVY
                     MVIGFVALMLSYFGTKERIIPTAEETDKVKYSDLVNELKRNRPLQILGLFFLIGFTFM
                     FFGNTVWPFYLKYNIGHSEWIASVGLLGSIPGIFLVFLWPKLRRTIGKKNFFYLFLGI
                     FVLGQLCLWVWQFDSFKYSPTLGYIGRFLQQWGLTSATGFMWALVPEVVTFGEFKSKK
                     RVAGIINALMGLFFKIGLALGGIIPGYINAVYGFNGSLLAQTEEALFGINLSMIWAPI
                     ILAIIAGFVMSRYPLSDTDIDHINEKITAYSK"
     gene            4680..5726
                     /locus_tag="PXB44_001569"
     CDS             4680..5726
                     /locus_tag="PXB44_001569"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL5282036.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5729..6367
                     /locus_tag="PXB44_001570"
     CDS             5729..6367
                     /locus_tag="PXB44_001570"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL5282037.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            6386..7366
                     /locus_tag="PXB44_001571"
     CDS             6386..7366
                     /locus_tag="PXB44_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5282038.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(7428..8429)
                     /locus_tag="PXB44_001572"
                     /pseudo
     CDS             complement(7428..8429)
                     /locus_tag="PXB44_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            8370..9380
                     /locus_tag="PXB44_001573"
                     /pseudo
     CDS             8370..9380
                     /locus_tag="PXB44_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(9467..9766)
                     /locus_tag="PXB44_001574"
     CDS             complement(9467..9766)
                     /locus_tag="PXB44_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282039.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(9753..>9945)
                     /locus_tag="PXB44_001575"
     CDS             complement(9753..>9945)
                     /locus_tag="PXB44_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL5282040.1"
                     /translation="PEQKVKVFFSIAIIMFIAVTICGMMILSALKKQRHFMQSTLTEL
                     KLDIAAFKSALTLKQNGEH"
BASE COUNT         2919 a         2015 c         2151 g         2860 t
ORIGIN      
        1 ttagatctta acctctctaa aaatctattt cccagatgat ccctttttac gctaaaatat
       61 gcgttcattt ttcagcagaa tttgtaagat agtagaagag atatgattta caaccaaatt
      121 tacgatgtga tcgtggtcgg cggtggtcac gccggcacag aagcggccct agcaccggca
      181 cgaatgggct taaaaaccct gattttaacc cacaatgttg ataccttagg gcaaatgtcg
      241 tgcaacccgg ctatcggagg gatcggcaaa ggccatctgg ttaaagagat cgatgcgatg
      301 ggcggtttaa tggcaaaagc aacagacgtt gccgggatcc aattccgtac cctaaacaac
      361 tcaaaagggc ctgcggtgcg tgccacccgt gcccaagctg accgtgtgct ttaccgtaat
      421 gcggtacgca ctgcgttaga aaatcaacca aatttagata ttttccagca agaagtagtg
      481 gatattttgg tggaaaacaa ccgtgcagtg ggtgcagtta ccaaaatggg cttaacgttt
      541 aaagcccgtg cggtagtttt aaccgctggt acgttcttag cgggtaagat ccacattggg
      601 ttggataact atgcaggtgg tcgtgccggc gatcctgctg caacaatgtt ggcagatcgt
      661 ctgcgtgatc tgaatttgcg tgttgatcgc cttaaaacag gtacaccacc tcgcctcgat
      721 gcccgtacca tcaactttga cgtacttgcc aaacagcacg gcgatgaggt gttacctgtc
      781 ttctctttta tgggcgatgt ctcacagcac ccacgccaaa ttccgtgcta catcacccac
      841 actaacgatc aaacccacga tttaattcgc aacagtcttg atcgcagccc gatgtacacc
      901 ggtattatcg aaggggttgg tccacgttac tgcccgtcta ttgaagataa agtaatgcgt
      961 tttgccgatc gtaatagcca ccagatctac cttgaaccgg aaggcttaaa tacgattgag
     1021 gtttacccga acgggatctc caccagcttg ccgtttgatg tgcaaatggg gatcgtcaac
     1081 tcaatgaaag gcttggaaaa cacccgcatc atcaaaccgg gctatgcgat tgagtacgac
     1141 tacttcgacc cgcgtgattt aaaaccaacc cttgaaacca aagcgattga cggtttattc
     1201 tttgccggac aaattaacgg cacgaccggt tacgaagagg caggtgcgca aggtttgctg
     1261 gcagggatta acgcagcgtt acaggttcaa ggtaaagagg cgtggtatcc aacccgtgat
     1321 caagcctaca ccggcgtgtt ggtggacgac ctctgtacat taggcaccaa agagccatac
     1381 cgtgtgttta cctctcgtgc cgaataccgt ctgttgctgc gtgaagacaa tgcggacatt
     1441 cgtttaacac caaaagccca cgaactagga ttaattgacg aggcacgttg ggtgcgtttc
     1501 aaccaaaaaa tggaagcgat tgagcaagaa agagctcgtt tgaaatccac ttgggcacat
     1561 ttgcaaatgc caaatctgga cgaattgaac gcgttattgg ctaatccttt agcccgtgaa
     1621 gcaagcggtg aagacttaat ccgccgtcct gaaatgagtt acgaaaagct gacccaaatc
     1681 gccctatttg cccctgctat tgaagacaag caagtggcag aacaggtgga aattgcgatc
     1741 aaatatcagg gctatattga gcatcaatac aacgaaatcg aacgccataa acgccacgaa
     1801 aacaccctga ttccggtaga gtttgactac gacaaagtcg aaagcctctc caacgaagtg
     1861 cgagctaaat tgatgcaaca ccgccctgtc tctatcgggc aggcaagccg catttccggc
     1921 atcacaccgg cagcgatttc cattctgttg gtgaatttga agaagcaagg aatgttgaaa
     1981 cggggtgagc tgtaaatact acaagcggtt ggttttgcag aaatttttgc aaaattgacc
     2041 gcttgtattc tttggagaag gaaatacccg aactaccgcc gtatttttaa tgatatatct
     2101 gcgtaaactc ggtttttcgc cgttcgatca ccaaatgttt gcccaacctt cgtggtattt
     2161 ctgcaatgca cttgttcata ccaattatga aaatctgcga caaggtgtat ttgtgactaa
     2221 agcgtattta gtgatgtttc ttgaaaattt attgtttgaa aaaagtcatt cgttaagtaa
     2281 tcgggcattg cgtattaatg aaaataatga ttaaggaatt tgcaaaatgt tagctaaatt
     2341 aaaccgcttg cttgctcaag ccgaaataaa tttgaccgat caacaaaaag agcagttagt
     2401 tggttttgtg agattattgg ataaatggaa taaggcgtat aacctcactt ccgttcgcaa
     2461 ccctgatgaa atgttggtta aacatatttt ggacagcctt gttgttagtc gtcatttaca
     2521 aggcgaaacg tttattgatg tcggcacggg gccggggcta ccgggcattc cgctggcgat
     2581 tgctaaccct gataaacagt tcgtgctact agacagctta ggcaaacgca tcacttttat
     2641 caaaaatgcg ttgcgtgagc ttggtattaa aaacgttacc ccagtgcaaa gtcgagtgga
     2701 ggaatacact gaaagccagt ttgacggcgt attaagccga gcctttgcct cgcttaatga
     2761 tatgatcgac tggtgttatc atttaccgac tgaaaacggt aaattttatg cattaaaagg
     2821 cgtgtatgat gagcaggaaa taattgaaat caaacaaccg attcagctag tggaagtgat
     2881 taagcttgaa gtacctgagt tggtaggaga acggcattta gtagtgttga aaaagcccta
     2941 atacattgga agtttaatga tacaagcggt cgaatttgta aatttttttg caaatctgcc
     3001 tgcttgtgtt aactattttt cataaagcat acctggttaa taaggtgtgc tttttttata
     3061 acctattatt tcatctgatt tatttctatt ttgtgatgtg cttcacaaaa acaaaaaaac
     3121 ataatgtagt aaaaaatatt cgtaattgaa gcccttattt ctttacctga aaataagcga
     3181 acttaattat ataacgagga ggttcgtaat gaacagggta acaaataagg cagatgagtt
     3241 tgctaaactc agtacgtttg aacgtattgc ttatggtagt ggcgacttag cccaaaattt
     3301 agtttttggt acagttggtg gtttcttact cacttactta actactgtaa acggcatttc
     3361 tgccgtagca ggagcaacga ttttcttaat tgtaaaatgg ggaaatgtgt tttgggatcc
     3421 gtttgtcggt gcctttattg acaaaagcaa gccctcagca cagggtaaat accgcccgta
     3481 tttactgaaa tttgggattc ctcttgtggt tttagcttca ctactatttc ttcctcttga
     3541 atcagtacgt ggctcagtga tttatgcttt cctttcttat tttgccactg cgatggttta
     3601 ttcttttgtt aatattcctt atggctcgct ttctgcttct cttactcgag acacagacga
     3661 aattgctaaa ttaaccacaa cccgtatgac aatggcaaat atcgctaatt tgtttgttta
     3721 taccttattc ccactttttg tgcagttact tgcaccacat gccgaattaa aaaacaccgg
     3781 ctttttcggt attgagctct atttaggtga ttatatgtct gctgctgcag gccctgcttg
     3841 gtttaaggtt tatgcggtct atatggtaat tggatttgtg gcgttaatgc tctcttattt
     3901 tggtactaaa gagcggatta ttccaacagc ggaagaaaca gataaagtca agtattcaga
     3961 tttagttaat gaactcaaac gcaatcgtcc attacagatt ttagggttat tcttcttaat
     4021 aggttttact tttatgttct tcggaaatac ggtgtggcct ttttacctaa aatataatat
     4081 tgggcattct gagtggattg cctccgtcgg tttattaggc tctatccctg ggattttcct
     4141 tgtattctta tggccgaaac tacgtcgcac tatcggtaaa aagaactttt tctatctatt
     4201 tttagggatt ttcgttctag ggcaactttg tctatgggta tggcaatttg actccttcaa
     4261 atactctcca accttaggct atatcggtcg tttcttacaa caatggggat taacttctgc
     4321 taccggtttt atgtgggcgt tagtgcctga ggttgttacc tttggagagt ttaagtcgaa
     4381 aaaacgtgta gcaggtatta ttaatgcgtt aatgggcttg ttctttaaaa tcggcttggc
     4441 gttaggcggt attattccgg gttatattaa tgcggtttat ggttttaacg gctcgctttt
     4501 ggctcaaacc gaagaggctt tatttggtat caatctttca atgatttggg ctccaattat
     4561 tttagcgatt attgcaggat ttgtgatgag tcgctatccg ttaagcgata ctgatattga
     4621 tcatatcaac gaaaaaatta ctgcttattc caaataattt gagtatagga gaatagatta
     4681 tgcaaattca aaatccagtt ttacccggtt tcaatccaga tccatctatt gtgcgtgtgg
     4741 gtgatactta ctacattgca acttctactt ttgaatggtt tccgggagtg agaattcacg
     4801 aatcgaaaga tttagtgcat tggaatttag ttaaaaatgt cttagataca accacgttgt
     4861 tagatatgcg aggcaatccc tcttcaggtg gtatctgggc tcctgatctt tcttatgcgg
     4921 aaggtaaatt ttggttggtt tataccgatg taaaaattac agacggtgca ttcaaagata
     4981 tgactaacta tctaactact gccaaagaaa ttcaaggtcc ttggtcagct ccgattaaat
     5041 tgaatggtat tgggtttgat gcttcgttgt ttcacgatga tgatgggcga aaatatttag
     5101 tgcaacagac ttgggatcac cgtgaatatc atcacccctt tgacggtatt acgttaacgg
     5161 aatttgatgt tgccacaatg aaactcaaac ctgaaaccca gcgtacgatt tttgaaggta
     5221 cagaagtgaa attggtggaa ggtccacata tttataaaat taatggcgaa tattatctgt
     5281 ttgcggcgga aggcggcaca gtatttacgc atcaagaagt agtggctcgt tctaaaacat
     5341 tggaggctca ctctttccaa ctggctccaa atacaccgtt tattactaat tttgatacac
     5401 ctaattctta cctacaaaaa caagggcacg gttcattagt tgatacccca agtggtgaat
     5461 ggtattacgc ttccctcaca gcaagacctt ggaatcatgc aacagaatcc gcaaccgatc
     5521 ctcgtggttg gtcaacatta gggcgtgaag ccgccattca aaaagtagaa tttgatcaag
     5581 acggctggcc ttatattgtg ggtggacacg gtggaaaaac ctttgtagaa gccccaaaag
     5641 atgcaatttt taccgaagct cctattgatc attctcaaca cgatgagttt gatagctcaa
     5701 cgttagatat taactggaac acttaagagt gccttttagt aaagagatgg gcgaagttgg
     5761 taatggtgtt ttaaaattga ttgggcgagg ctctcttgca aatacacacg atttatcgct
     5821 aatcgcacgc cgttggcaag ccttctattt tgatgcggaa accaaagtga aatttacacc
     5881 gttttcttat caatctatgg caggtttaac taactattac aatcactcac actggtcttg
     5941 gattttcatc accaaaaatg atcaaggaca acaggtgatt gaagttgcag aaaataaagg
     6001 tggactcaga aatgggcaat atacttctta ccttaaagat aaagcaattg tcattcctga
     6061 tggaacggag tatgtttggt ttaagactaa agttcggaaa gaaacttacc gttatgaata
     6121 ttcattcgat ggcaaacaat ggcacactat tccggttgaa ttagatgcgg cagtgctttc
     6181 agatgattat gtgttacgca gttatggcgg tttcttcaca ggagcttttg tcgggctggc
     6241 tgcagtggat tactccagtt atgaatctac agcagaattt agctactttg actataaaga
     6301 gttaggcgat aagcaactta cagacggctc attctcttgg gaaaaatctg aaagttgctt
     6361 tggctaaaat gaggagaata tcacaatgaa acaacgcaga tttggtaaaa ccaacaaaat
     6421 ggtgtcagaa attgggttag gcacttggca gcttgggacg aaatggggtg aaccgttcaa
     6481 tcatcaagag gcgatggcaa ttttagaaac cgcttatgag caaggcatca attttattga
     6541 taccgctgat gtgtataaca acgggcaaag cgagaaagcc attggtgata ttttgaaaaa
     6601 ataccctgac tttttctatg tcgttaccaa atgtggacgt gcgttaaacc cgcatactgc
     6661 tgaaatgtac acgccacagg caatcgaaaa atttgttgat ggtagtttaa gccatcttgg
     6721 cacggaaaag ttggatatga ttttgctcca ctgcccgcca acgttggttt atcgcaatga
     6781 tgagattttt acgaaattgg aacagcttaa agccggcgga aaattaatag actacggtgt
     6841 gagtatcgaa acggcagagg aaggcttgtt ggcaatggag tatgacattg cggcaatgga
     6901 agttatcttc aatatgttcc gcttaaaacc attggagcaa ctcttcccaa ccgctcagca
     6961 aaaaaatgtg ggtattatcg cccgtgtact gttggcaagt ggtttgctta caggcagata
     7021 taatgcctgc acccaatttg gcaaagacga ccaccgcaca tttaaccgca atggtgaagc
     7081 cttcgataaa ggcgaaacct tttcgggtgt ggattatcaa ctcggtttac aagcggtgga
     7141 agaattgaaa gccctattcg gcacggaaga tttaattcct tacgccctac gttgggtatt
     7201 gatgaatgaa gctgtgagta cagtaatccc gggagcaagt aaggtttcac aagtaattac
     7261 taacgcagcg gtggataatt tccctccact cactgaaaat gaaatgcaaa aagtggaaga
     7321 tatttacaac cgcttaattc gcccaagtgt gcatcatttg tggtaattta tccaacgcta
     7381 taaaaaatgg aactctaact tgagttccat ttttgtcaat tttagcctta aacctctaac
     7441 tgtttagagg ttttcttaaa cagagtcaat gcaataaacg caaaggctaa gaaacaggaa
     7501 aggatcaggg catagaaacc accgtcccag ccgaacatat caaccagttt acccatcaca
     7561 taacctgcac ttgcagagcc taacaagtag ccgaataaac cggttaaacc tgttgctgta
     7621 ccggtggcaa cacgaggcac taaatccgct gcttgtaaac cgatcatcat caccggcccg
     7681 tagattaaga aaccaatcgc cactaagcag atgttgtcga ttaacgggtt gccggctggg
     7741 tttttccagt acaccacaat cgcaattaac acaccagcaa ggaataacaa catcggcggc
     7801 gcacggtgac ctttgaatac tttgtcgctt aaataaccgc ttgcaagcat accgaagata
     7861 ccggcatatt catacaagaa gtacgcccag ctctgtttat ccacagagaa atgtttcact
     7921 tcttttaagt aagtcggtgc ccagtcgata atgccgtagc gaatgaaata gacaaacacg
     7981 ttggcaatcg caattgccca caggaatttg ttgtttaaaa tatatttgta gaaaatgtct
     8041 ttggaagaga gtgtgtgggc agactccact ttttccacca ctttctcgcc tttgtactca
     8101 tcaatcggcg gtaagccttg agattccggc gtatcacgca tcaaatagaa catcacaaac
     8161 gccagtacaa tcgcaatcaa cgccggcaga tagaacaggg attgccacac gccaaaaata
     8221 gataagccta aaatcgccaa cggcccgatt aaaccgccgc ccaagttgtg cgacacgttc
     8281 caccagctcc accacacgcc acgttcagag gttgagaacc agttggtcat cgttttcgcc
     8341 cccggcggat agcccattcc aaaacgccac aaggcaaaat tgatggggtg actttctacg
     8401 atgaactcaa tcaacaagaa catactgttt tcggcactgc ggtgattaac gcaaccggcg
     8461 tatttatgaa tgatatttta agtatggatc acggtagcac gaaaaaattt gtcgtaccaa
     8521 gccaagggat tcacttagta ttagataaat cattcttacc aagtaatgat gcgttaatga
     8581 ttccaaaaac ctctgacgga cgagtgttat ttgctgtacc ttggcacgaa gtgttagtcg
     8641 tagggacaac agatactctg attgaaaatc cttcttatga gccaattcca ttggaacaag
     8701 aggtgaactt tattttagag acagccgcac aatatctgac aaaaaaacca actcgtgatg
     8761 atgtaaaagc agtctttgcc ggtcaacgtc cgcttgcggc accggaaaaa gaaggtcaaa
     8821 gcactaaaga agtatctcgt agtcacaaag ttctcactga tgcaaccagc ggtttgatta
     8881 cgattacagg tggtaaatgg acaacttatc gtcaaatggc agaagatacc gtagaagaag
     8941 cgttaaaagt ccactctgaa ctaggcaata aaccttgtgt gaccgtcaac ttaccgattc
     9001 acggtaatat tccggcagca caagtgaatc tacaagatca tctatatgta tatggctcag
     9061 atattccaca gttacgcgcc ttaatgactg agaccccgga atatgcacaa aaaatccatc
     9121 cgagactaga ttataccgtt gcagaagtga tctgggcagt tcgccacgaa atggcacaaa
     9181 cggttgagga tgtattagct cgccgtgttc gtttgctctt cactgatgcg agagcggcga
     9241 ttgatagtgc agcaaaagtg gctgctatta tggcgaaaga attaggtaaa gatgaaatgt
     9301 ggcaacaaca gcaagttatc cagttccttg atgttgcgaa gcactattta ttggtagatt
     9361 atcagccaca gccaatctaa tacgctaaaa gcaagcggtt aaaatcgata aaaatattgc
     9421 aattacgtta tttgcaactt tttaaaccat tttaaccgct tgtatattag ctatcatctc
     9481 ttttatgttt ctgttgtaat aaaaagaatg ctgttaaccc caatacagaa aagctaattc
     9541 ctttcatact caataatctt tttaccaaaa aatctaatgc aatggtttta actgccggtg
     9601 aattgaccag tgcaagccct gtttgtttca cttgtttaaa aggctgcata atctcatttt
     9661 tggttttatt ggtttccgct ttaaatcgca ttctaagtgc attacctttt agaactagaa
     9721 gttccttttc ggttaattta agctcttcca ttttaatgtt ctccattctg tttcaaagtt
     9781 aaagcactct taaatgcggc aatatctaat tttagctcgg ttaatgtact ttgcataaaa
     9841 tggcgttgtt ttttcaaggc agaaagtatc atcattccgc aaatagtcac agcaataaac
     9901 ataataatgg caatagaaaa gaatacttta actttctgtt cgggt
//
LOCUS       DANLUA010000089         9864 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLUA010000089 DANLUA010000000
VERSION     DANLUA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            170..1876
                     /locus_tag="PXB44_001576"
     CDS             170..1876
                     /locus_tag="PXB44_001576"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL5282041.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1925..2764
                     /gene="menH"
                     /locus_tag="PXB44_001577"
     CDS             1925..2764
                     /gene="menH"
                     /locus_tag="PXB44_001577"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL5282042.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2813..3490)
                     /locus_tag="PXB44_001578"
                     /pseudo
     CDS             complement(2813..3490)
                     /locus_tag="PXB44_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3576..4238
                     /locus_tag="PXB44_001579"
     CDS             3576..4238
                     /locus_tag="PXB44_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL5282043.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4228..6156
                     /locus_tag="PXB44_001580"
     CDS             4228..6156
                     /locus_tag="PXB44_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL5282044.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6156..7043
                     /locus_tag="PXB44_001581"
     CDS             6156..7043
                     /locus_tag="PXB44_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL5282045.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            7001..8107
                     /locus_tag="PXB44_001582"
     CDS             7001..8107
                     /locus_tag="PXB44_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL5282046.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8104..9528
                     /locus_tag="PXB44_001583"
     CDS             8104..9528
                     /locus_tag="PXB44_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282047.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9643..9849
                     /locus_tag="PXB44_001584"
     CDS             9643..9849
                     /locus_tag="PXB44_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL5282048.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3051 a         1989 c         2126 g         2698 t
ORIGIN      
        1 ctcttaaaaa agagagagtt tactaagtaa agtgttgctc aatagataga aattttcacg
       61 aagttttaca aaaacattcc aattttctcc taaaaaactt cccacaataa tttcactttt
      121 tgctacaatc ctaaccattt atcgaattta tcaatcagta gagaagaata tgaataccag
      181 ttcttttaat cgcacttggg caaaagtgat tttaagtgca ttaatgcgtt atggcgtaaa
      241 acatttttgt attgctccgg gttctcgctc aacaccgctc acattagcgg cagtgcaact
      301 tcaacaaacg caatatgcag agtgccacac tcattttgat gagcgtggtt taggttttta
      361 tgcgttaggg attgctaaag caaccgacag cccggttgct attattgtca cttccggcac
      421 agcagtagcg aacctttatc ctgcggtgat agaggcaagc ctgagccacc aaaaactgat
      481 tattctttct gccgaccgcc caaccgaatt aatcggctgt ggggtaaacc aagccattca
      541 tcagcaaggt atttttgcca attacccggt tgccagtgtc aatctaccga aagcgaacga
      601 ggcttaccac gcaagctggc tggtttcgac cattgagcaa gcctgcacta cccaagcaca
      661 acaaggcggt gtgttgcata tcaatgtgcc ttttgaagag ccgctttacg aagcagacat
      721 taatgaaatt ctaaatacac cttggttacg cccaatccaa ggctggttga ataacgctca
      781 aaccaaatgg atagaccaac aagcgattca aagcgaagtc tcaatgcacg aaaattggga
      841 ttactggcga actaaacgag gggtgattgt ggtcggcaaa ctgccgccgg aacaaggcac
      901 aggtttaaaa acttgggcgg aaaccatggg ttggtgcttg ctaagtgatg tgcaatcttg
      961 cattgaagcc agcctgccgt atgccgatat ttggctctcc aacgagactg tcaaacagcg
     1021 tttattgcaa gcggatatcg tgattcaatt cggcacgcaa attgtcagca aacgggtgaa
     1081 tgaattttta aaagcattcc aaggggaata ttgggtggtg gaacaatcgc acgattacca
     1141 taatccaaac gctcatcatc aaacccgttt tattgccaaa gcacatcact ttttgcgggt
     1201 acacccacca cttcgccaaa agccatggtt gcttgagcca ttggcattat cgcaattctg
     1261 ctccactttc atagagcaac aagtgggtgg cagcttaagt gaagcctcac ttgcacacaa
     1321 cattcaagat gttttagcaa ttaatggcaa cttatttatc ggcaatagct tatttgttcg
     1381 attggttgat gctctgtgca aactgccgga aggctaccct gtttacacca accgtggtgc
     1441 aagcggtatt gatggcttaa tcgcaacaat ggcaggcatt gcaaaaggta gcgaccgccc
     1501 gactgtcggt gtggtaggtg atatttccgc attacacgat cttaactcaa ttgcattgct
     1561 caccaaaatt aaccagccga ccattttatt tgtggtgaat aacagcggtg gtgcgttgtt
     1621 tgatatgttg cctgttgata gcaaagcgaa agatcaatat taccgccttg ctcacaactt
     1681 tgaatttgct caagtcgcca caatgttcgg gcttgaatat atccgcccgt ttacttgggc
     1741 agatctaaaa accaaactca aacacgccta tgctcgtaaa ggtgttacca ttgtggaaat
     1801 caaagtaaat gaacacgacg gcagcaatat gtataaatca ttgctaaaac aaatttcaca
     1861 ggcggaaatt gcctaatcac aactgcccct cgccaatcct gcgaggggta aaaatgtatc
     1921 gactatgctt tcctatcaat ggcacgctca acaaggcaca cccgtggttt tccttcacgg
     1981 cttgctcggc tcacaacaag actggcaagc tgttctccaa catttgcaaa attttccgca
     2041 aattcgaccg cttaccattg accttccttt tcacggaaat agtaagcaca ttacctgtgc
     2101 cgattttgcc aacctgcgac aacaactaca cgccaccctt gaaagcctaa tcggctcacg
     2161 acctttctat ttagtcggct actcactcgg tggacgagcg gcactcgatt atgtgttaaa
     2221 tatggagaat ccgaatctgc agggcgtggt gttagaaggg gcgaatatcg gcttaagcac
     2281 agaaaacgac cgccagattc gccggcaaaa tgaccgccat tgggcaaacc gtttccgtca
     2341 agaacccatc gaacaagtgc ttgaagattg gtacaaacag cctgtttttg cggatttaag
     2401 cgatacggaa agagaatgtt atattgatag cagaaagcac aatcaaggag agcaaattgc
     2461 tcaaatgctg gaagccacca gcctcgccaa acagcctgac tacaccgaaa aactcaagca
     2521 gacggataag aatattgtct tttttatcgg tgaaaaggat cagaaatttc gcaaaatggc
     2581 atcaagccac caactaccaa cacaactgat tgctaatgca gggcataacg ctcatcgggc
     2641 taatgctaaa aattttgtgg agaaattggg gttattgata ctccactccg acagcgacct
     2701 acggtcgcaa tcggttaagc ataacggtgc agctctgctg cacaaaagcc ccagtggggc
     2761 ttaatcagcc gtaggctaaa tataacctag tacggctcag ccgtgctagg tgttagggct
     2821 ccccctgcaa ccgttgcaat aagcgatcca tcgcccgata accaagtgct tccgctaaat
     2881 gcagttgttg aatattcgcc tcgccggcta aatcagcaat agtccgagaa actttcaaaa
     2941 tacggtggta ggctcgtacc gataaaccga gctttgtcaa tgcagtttct aaaaataggg
     3001 catcttgtgc cgataattgg caatctcgct caatttctcg ggtggtgagt tgagaattga
     3061 ttttacctgc tcgctcaaat tgcatttttc tggctaaaaa cactcgcttg cgaacctgct
     3121 cggtggtctc tcctcgattc tccgtcccac tttgcaatgc tccttgtggc aaaagtggca
     3181 cttctataga gagatcgaaa cggtctaaga aaggtccgga aaggcggttt aaataccgca
     3241 ttacctgttg gggcgaagtg cggttatgcg tgccttgata atgccctgtc gggcttgggt
     3301 tcatcgctgc aattaattga aagcgtgccg gaaattgtac tttagcgttg gctcgggaaa
     3361 tcacaatttc gcctgcctcc aacggctgac gtaacgcatc aagcacttta cgttcaaatt
     3421 caggcaattc atctaagaac agcacaccgt tatgggcaag tgaaatttcc cccggtttgg
     3481 ggatcgagcc actgtaaaaa ctagaaataa atgtcataaa aactagaaat agctgtcaga
     3541 ttatttagaa aacaagaaat aggtgtcaaa ttattatgag aaaaacacat agttttcaac
     3601 aggttagaaa aattaaaccc acttggatga gtgtatcggg gcatattcca tttagaaacg
     3661 gagtcagtat tccctatgaa tcgactttag agcgtgattt tttgatgtat ttcacttatc
     3721 taccttcagt agatgaagtc gtctctcaac cgactaccct acctttcgtc aagaatggga
     3781 tcacttatac ctatacacca gatttcttct tgtcatttac agacggtcga aagccgatgt
     3841 taatcgaggt caaaccgaaa gcaaaatggc agaagcactg gaaagaatgg aaagaaaaat
     3901 ggaaagctgc aatacgtttt tgccaagaaa acggttatgt attccatgtg tatgatgaag
     3961 atcggattcg acatctagcg cttttcaatc tcaactatgt tcagcgctat aagcgaatac
     4021 aacatgaaca agaagatata gatgctattc tagcccaagt aaagctgatg ggaaatacca
     4081 ctattgacta tttactctct cgattttttg caggttctct atatcgcatg aaaggcctac
     4141 aaatcatcta tcacttactg gcaacgaaac aactacattg caattggttt ctaccactta
     4201 atgaatttac cgaggtatgg gggaataatg atgagtaact ctggatttac agaaaagcgc
     4261 tatcatcacc gattagatcg gggacgtatt attttgcaaa aaggtaatat ttacctaaat
     4321 agagaagatg gagaacagta tgaattggtt gattatatgg atgaaccctc ccaactgctc
     4381 gtacgaaatc tcaacactcg tacaaccaaa gtcgtcagta ttcatcagct tgaaaatttc
     4441 aaaatgaacg agcgaacgga tctttctgtg gatgtaaccg ctattagtaa tgaatattgg
     4501 gagaaagccc aacaaaaata tgaggcaatt aaaccgttat tagggatgga tcaacatcgg
     4561 ccttacgcag ttaaggctcg tgcagaagat gttggggtga accctcgtac attataccgt
     4621 tggttacaag cctataattc cataggatct atcgcaggtt tagtcgatca aaaacgtgga
     4681 tggcaacaag gaaattcccg tttaacgcca gagcaagaca agctgatcgt gcaggtaatc
     4741 aatgagtttt acctacataa acaacgccca actactgagc aaactattag agaaattcgc
     4801 cgccgatgca aaattgaaaa agtggaaagc ccaagcaaag aaaccatccg tatgcgcatt
     4861 ttgcatattt cagaggaaga gcgattacgg aaacgcgggc aacgagaaaa ggcacgcaac
     4921 aaatttaagc cgaagccaaa tagctttcct gatgcggatt atccattgag tgtggtacaa
     4981 attgaccata caccagttga cctaattatt gtggatagta agtatcgtaa accgattggc
     5041 cgaccgtttt tgacggttgc gatagatata tacagcagaa tgattgttgg ttattacctt
     5101 tcacttgatg cgccttctgt aacttcggtg gcgatgtgta ttgcacgtgg tattttgccg
     5161 aaagaacgcc tactgcttga tttaggtttg caaggctcag aatggaatgc ttttggctac
     5221 ccagtgaaag tgcatgtgga taatggccca gattttcagg ctttggattt atctaaatct
     5281 tgttctgcac acggaatcca tcttgaattt agaccgatgg ggcgacctga atatggcgga
     5341 catattgagc gtgttatcgg tacatttatg aaagaagttc acagtttagc aggaacaaca
     5401 ttctccaaca tcaaagagcg tgactcttac gattcggaaa aagaggccat tatgactctc
     5461 gatgagtttg aaaaatggtt ggtacattac attgtgaatg tttaccacaa acgtgtgcat
     5521 tctgctttag gcatttctcc tgaacaaaaa tggaagattg gtatttttgg agatgaaaat
     5581 gaagtgggat gtggttatcc gcaacttcct gttgatgaac aaacactact acttgatttc
     5641 ttgccaagta tcactcgtac cattcagcat aatggtgtaa caattgacgg acttcgttat
     5701 tacgatgttg cgctaaatat gtatatcagt gattcagatg aaagcggtaa atctaaggaa
     5761 tttctgtttc gccgagaccc gcgaaatatc agcaaaattt ggttttatga tccgaaatta
     5821 aaacgttact tcccaattcc atttgcaaat caggcaatgc ccgaaatgag catttgggaa
     5881 tatagagagg ttcgtagccg aattgcgaat aaaggtgata aatatatcaa cgagcaacaa
     5941 gttttagacg gtttaaccga gatgcgtgaa atggttgctg aatcggctca acggacgaaa
     6001 aaagcacgcc gtcaggctga acgacaaaaa atgcacaaag caagtaagcc aattattgaa
     6061 acaaaagtgg aaactaaggc tgttgtacca gtcgtagtaa catcgaattt gcttgcttta
     6121 gatgatgaga gcttatcttt tggtgaggtg gactaatgca atacgaacat gttcatgaaa
     6181 aattccgcca tcttgtgaca gcggacaatc aggagaggat tgcgttcttg gacgagccaa
     6241 gatggttagg ttatggtgtc gccaaagaca ttatggataa tttggtttct ttaatgaata
     6301 aaccaaaacg cccacgaatg ttgaatttgt tgattgtcgg cgattccaat aatggaaaaa
     6361 ccacactgat ccgccgcttc ttcgacttgt acggtcaggc gtatatcgac agtgattcta
     6421 atgcgattta ccctattctt cttgccgaag ccccaccaag tgcgaatgaa aaagaattgt
     6481 acatctcatt attagagcgg ttctacgtac catataagcc gacagatacg atagccaaac
     6541 ttcgttatca gactatccat ttgttccgag aatttcgtgt caaaatgttg gttattgacg
     6601 agtttcattc tctgttggtt ggtacaccac gcttacaacg acaggtgatg aacgcaatta
     6661 agatgctttg taacgagcta caaattccaa ttgtcggtgt tggtactcgt gatgcaattc
     6721 gggtattaca tacggatcga caacatgcca gtcgttttga tgttgcggaa ttgccaatat
     6781 ggaaactaga taaggaattt caaaaactcc tatttcaatt ccaagggatt ttacctttga
     6841 gggagagttc aaatctgcaa tctccagaac ttgctacgaa gatccacacc atttcaggtg
     6901 ggaacttagg gaacgtgcat cgcttactaa cagcttgtgc agttcaagcc attacaagtg
     6961 gcacggaaaa aatcacctta gacatcattg agaaaaattc atgggtacaa ccaacaaacg
     7021 gctatcgcaa aataatcgga taaaaaaagg tgttattcat acccctattt attcaaatga
     7081 aagtattagc tcgtggttaa ttcgtgcatc ctttgattgt ggtacagatc cactgacttt
     7141 tacagggttt tattgggaaa aacatcggtt gtggacatta gatttagatc gtggcttgga
     7201 aaatattgag ccaactatct atgatgatgt acaggcatta tcgggtaaaa tccaactttc
     7261 agaatattcc ttgtttcgca ggctttaccc tataaatgca gatgaaacct tagctaaagg
     7321 tcaagtaaga tgggtgattc cacgttcggc aaggaataga acccatcaca ttggtcaaca
     7381 atattgtcct ttgtgcttgg atgagtcgcc acatttacgc aatgattggc ggttagcgtg
     7441 gaattttggt tgtctcaagc ataatatgat gttggcgaac agatgttttt gttgtggtac
     7501 attgtatcaa ccccatttac tatcagcgga aaagaggcat ttgaatcatt gtcatcaatg
     7561 tggtgggaaa ctggaacaag gcacaatgga aatgagtcta gctgaaaagc tgacattgca
     7621 aggtattaat caggtactcg aaaaagattc aggcatctgt tttggataca aggtgtcggg
     7681 taaggtgtgg tttgatatgc tccgctactg catcaactta ttgagacgtg catcagacag
     7741 accaaatcat gccttagcaa aatttgtagt taatctacag attgatgttg aaaatatatg
     7801 tagccccaaa acaggacttt cttttgaaca acttccgcta ggtgaacgta caaatttact
     7861 tgtgaatgcc tatcatattt tgggaatgga agcagattct attcgtagtg caatttattt
     7921 gtgtggcgga acacaaaaaa cctttatgtt tgaaaaatat cctgattttt ggcaagaatt
     7981 tatggcacta gcacccgcag gcccaaatag aacacgaaaa cgcaccaagc cccaaaatga
     8041 gtctatcaca gcaattaatc gccgttggga gcgtttgaaa cgcaaactac acttaaataa
     8101 cgaatgaaaa ttaatgtaat aaaaccacag gaagaacctt gctccgcttg tggcaaaatt
     8161 tctcgcattt ggcgcagata taatggcgaa ggctactgtg ccaattgcta tgctaagtgg
     8221 tttccgaaaa aagagtgcag ctgttgccat caaccaaagc gtatctattt gagttcaaca
     8281 gtatgttggg attgcgaaaa acagactgac tgtgtacgtt gcggtaaggc tgcgggtaca
     8341 ttcaaaattg gtaaaattac ccgttatggt gcagtttgta atgtgtgttc acgttatttt
     8401 cgtgaagaac gagaatgttt tgagtgtggt caactatcta gaactatgct tcgagcatca
     8461 gagtcaggtc atagcgagct actctgcttg aagtgttttc aaaaatatcg gtttgcgact
     8521 tgtaaaaact gtcgccgtta tcgcaaagtt catgaccgtg ataatcagct ttgtcgaaaa
     8581 tgtagtgagc aacgaacttc acaatgcccg aagtgcaaac aggatatgcc tgcgggatat
     8641 ggtaatattt gtgcagattg ttctaggcga actctactgt tcaaccttat tcgaatgaat
     8701 gtgcatttat tccgccacaa aaaagtgaaa agagcatata aaaagttcat tttttggtat
     8761 ctcgcaaagt gtggcgtgag tgttgtgctg aaaaaatcag acgaccacgt cctctttttt
     8821 atgcgttgcg atgaaaattg gggcaacatt ccagactacc gtgagttggt tacattcttt
     8881 aaacccaatg gattacggac gcatttgact gtgttgcgtt ggttgcttga taccaatcaa
     8941 gtcaaagtag atgagagctt aaaagaggaa atggcggggt tggaacgtat tcaatcccta
     9001 tttaacaaat taagtaactc tgtaccttgt attgcaaact actacacgca gttacaacat
     9061 aaatatgagg cgggcaaaac atcactgaaa tctgttcgat tagctttaca acctgcgatt
     9121 gatttcatca gttcagaatc tgtctcgtta ctgccgactc aagtacaact gaatagctat
     9181 cttgtggcaa aaacgggaca gattgctgct attacaggct ttataaacca cctgaaccga
     9241 gaatatcatt gtgaattaga aattgaccgc tccctcattc aaaagatgaa agaaaagcag
     9301 ttgaagcagc aagcaaaaca gcgattagcg atactctaca agcaagaaaa actaagtatc
     9361 aaagagcaaa atgagctact ctcagtcgta ttactgaatt tgcacaacat ttggataaaa
     9421 tcaccgaaaa aattaagtat ttcagagata gacggtatag cctactatgt tgataatgag
     9481 aaaaaatact ttataccaaa agacattcac atacgcctga tggaataagc tgtcgtcaat
     9541 ttttattttt gcgatctgtg tcgagaaaat aatatttatg aatcgacaga tgaaataata
     9601 ttgataaagt ctctttgtgt gttcaacaaa caggagataa atatgacatt aaaggctcaa
     9661 tttcgtgaat ggcttaaaaa agagcaagaa catgatgaaa tgatagggtt cgcccatatc
     9721 ccttgcattt tatctaaagt ggatagcaaa ctggcgattg ttagcggaaa tgcttatgag
     9781 aagaaatatc tcagatattg gatcaagtgt tttaggttcg atagaaatat cagcaagtat
     9841 attaaatagg ttaaatatct agct
//
LOCUS       DANLUA010000090         9860 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLUA010000090 DANLUA010000000
VERSION     DANLUA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(212..706)
                     /locus_tag="PXB44_001585"
     CDS             complement(212..706)
                     /locus_tag="PXB44_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5282049.1"
                     /translation="MLSGLKIESFSKEFKRDCFDSESELLNQYLKTQVSQDIKRRITS
                     CFLAVNSEQTVVGYYTLASTSLSLSDLPITLQRKLPRYPTVPAVLLGRLAVDKRVKGL
                     GLGQALLGNALKRVLRADIAAYALIVEAKDESAVNFYRKFGFIDFENEARKLFFPLDR
                     VRLS"
     gene            complement(706..978)
                     /locus_tag="PXB44_001586"
     CDS             complement(706..978)
                     /locus_tag="PXB44_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL5282050.1"
                     /translation="MATATARFEARMSQEVHMLLKRAASLEGRSLSDFVVGAALSAAR
                     KTVEQNDIIRLSVNDQELFAKALIDPPEPNLAMQKALALSSQILGE"
     gene            complement(1137..1565)
                     /locus_tag="PXB44_001587"
     CDS             complement(1137..1565)
                     /locus_tag="PXB44_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282051.1"
                     /translation="MRKLLVITALTLCTTPVFAADKNVIFSCTSTEGKPLTVKRVGND
                     YEYSYDKTTFKNPIKKAVTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKE
                     FIEPGATISQSKEQPSIGSVDCDTRKKSHYKFDVHLMNTL"
     gene            complement(1586..3592)
                     /locus_tag="PXB44_001588"
     CDS             complement(1586..3592)
                     /locus_tag="PXB44_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5282052.1"
                     /translation="MKSQFKFKKTHLLVITVLFSSTSSISFADSITDKLEFSKSDSII
                     SDRVFYQIGGGTGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMILQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSTQPDAKQAQKDLEDKKGKDGRVWVGGQRKGGQNQ
                     DPINVVGDVAKGGFNLLNKRNVLDSSPVSNCEGNLCQTWDTPQAAADFLTKIIGEQSI
                     STCKEDCGTKTSSKAGVGLSPEIEEEHIRTIVKLENVLNMDVPKSELLAELSSSSVPV
                     TRGLIESLRDDPDVELLSQRLSAEIATSKVMEKMLLARRAILAGMREPNVAQNADAQV
                     ELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLDKR
                     VQGLENGNVALTDLETGSNINLSRRNIALSIPNIPNVAALIPTLPNRGGSGVGGNFPS
                     LAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTVVKVTKDTVI
                     TKEDAERDLARRTAIFANNVRQELGDSHWNALPANAQAVLVSYAYNYGSLAKTKSVLD
                     AARRSAQTGDMTALATAVRNRQVDNNGINARRRNQEADYILGKN"
     gene            complement(3607..4548)
                     /locus_tag="PXB44_001589"
     CDS             complement(3607..4548)
                     /locus_tag="PXB44_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL5282053.1"
                     /translation="MKRAFFSVLLSVSLAVPTTLYPQQVKASLNTASIAASSASTSCL
                     DYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNQDGQTPWTELQSLNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGSTGYYCKSQTTPFIPYFLSGLDFFA
                     WRENMPEMFYPEALIPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     TRTAQPHIYFPAAKKGGVGEWPPGPVEEGQARTHKWQMLYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            complement(4545..4988)
                     /locus_tag="PXB44_001590"
     CDS             complement(4545..4988)
                     /locus_tag="PXB44_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL5282054.1"
                     /translation="MKASRFLGYFSIVTVVFSVVAQAQPNIIVYTTKMYPIQNSQLAS
                     QVYYLDQVENFEEEISRNLSKSPEIAEREIRAFFNSADWKAQEEQLKDAYQGVISGWQ
                     NGIKKVPAVFFNNLQSSPSVIYGMTDIQKALDEWHKWVQQHGESK"
     gene            5363..5716
                     /locus_tag="PXB44_001591"
     CDS             5363..5716
                     /locus_tag="PXB44_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282055.1"
                     /translation="MSQQTAGWDVIPFESSKTAKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFRALFVAIIAF
                     LCGMATVFGTQLSLI"
     gene            5788..6033
                     /locus_tag="PXB44_001592"
     CDS             5788..6033
                     /locus_tag="PXB44_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5282056.1"
                     /translation="MSVHIKLSCVGDILPIKLNEWTPEHRALHHEISHKALEAWQNRD
                     KGTQSVRFMQQMPARQGLHFRFLSVSEQDGKLIVSRS"
     gene            6042..6284
                     /locus_tag="PXB44_001593"
     CDS             6042..6284
                     /locus_tag="PXB44_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282057.1"
                     /translation="MFGKLFYKMNPKVLAKIGEDFRKAGYLMGAGFVGMVISNDQIST
                     SEGFVLFLWGLYIWIIGHIALHHSEKILNSKQGEPK"
     gene            6548..7390
                     /locus_tag="PXB44_001594"
     CDS             6548..7390
                     /locus_tag="PXB44_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282058.1"
                     /translation="MSDSVLNTYFKHALVKAGFPDTLDMEYSLSHCQGDGVAFYGRLG
                     TDDLIQLFNHLNPQKRKQKMFANLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMEL
                     YAKTADQLVFFNDSDARRNWYFPTSKVDKYKALWDGFIEDLEQYIKDISREMESVGYQ
                     IIEASPYQTETVFKFNTEKYLVELKRKPSEFYSELVDWAFGDMCDFEAAIEQICQGQL
                     CYADFEATVTDKKSEIQLGLARLSGISYSPNDRSFSGYRQELIGEAIASARHNAARFA
                     YLHA"
     gene            7505..7918
                     /locus_tag="PXB44_001595"
     CDS             7505..7918
                     /locus_tag="PXB44_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5282059.1"
                     /translation="MKNEHNLEQLKYIWDNRNLENKVYQYLFHICIEPMKKGDWNRYT
                     IWLNFHGNLINVSDVVYRVVESQKLKDDIKIWYDKQYYCRPTKVETTGFISIFLHLFY
                     EGIARILLEQGMITETEAAEKSYNFRAANCFQRLL"
     gene            7932..8087
                     /locus_tag="PXB44_001596"
     CDS             7932..8087
                     /locus_tag="PXB44_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282060.1"
                     /translation="MEPRTVKVIYDDGDYFFTLINADEQTIRNYYAIGSSIGFDNTRK
                     IVAVEIL"
     gene            8167..9150
                     /locus_tag="PXB44_001597"
     CDS             8167..9150
                     /locus_tag="PXB44_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5282061.1"
                     /translation="MNPQSNTQPKFDLYQEVTDKIIAALEQGTAPWLKPWDSVIPYGG
                     MPCNAISGRLYSGINVLLLWLSAAEHGYIQRKWITAKAAIEAGGHVRKGEKATLAVSY
                     RPYDREKTDDDGNLVLDDDGNPVIEHLASIKKHCLFNIEQCEKLPQDWYETVETPEQL
                     TDENQYAIFEEIRLMLKGMDLQVMVKPSNQAYYHPRNDHIVMPEMKQFHSPQKFHSVL
                     LHEMTHATGHQSRLARDGIVSGKARFGNKVYAFEELVAEMGCAFLCSHLGFADVPQNA
                     SYIESWIKVLREDKRAIFRASGKAREACQYMLDALNIAQLADKYEVFEEAA"
BASE COUNT         2885 a         1985 c         1916 g         3074 t
ORIGIN      
        1 tctcatgcat aaaatcagct aacagaacaa taccaattaa ggatataact gctccgaata
       61 cagcatataa ccatagcctt agctgataag ttattaggag aaataatata atatatgtat
      121 atttgagaaa agtgggatga ttccattctt taaaagctcg aattttcctg cctaaccaat
      181 aaccaaaatt atttttcata atcactctcc attaagataa tctcacccta tctagtggaa
      241 aaaataattt cctagcctca ttttcaaagt caataaatcc aaatttacga taaaaattga
      301 ccgcactttc atcttttgcc tcaacaatta atgcgtaagc tgctatatct gcacgaagta
      361 cccttttaag tgcattacct aatagagctt gccctaaccc taatcccttt acacgcttat
      421 ctactgctaa tcgaccaagt aggacggcag gtaccgtagg atatcttggt aatttcctct
      481 gtaatgtaat tggtaaatca gataatgaaa gacttgttga agccagtgtg taatatccta
      541 ctactgtctg ttcagaatta acagctaaaa aacaagaagt aatacgtctt ttaatatctt
      601 gtgaaacctg agttttgaga tattgattta ataattcaga ttcactatca aaacaatctc
      661 gtttaaattc cttgctaaat gactctattt tcaatcccga aagcattatt cccccagaat
      721 ctgtgagctt aatgccaagg ctttttgcat tgccaaatta ggttcaggag gatcaatcaa
      781 tgctttagca aaaagttctt ggtcattcac agataatcga ataatatcat tttgctcaac
      841 tgtttttctt gctgcagata gtgcagctcc aacaacaaaa tcactaagac tacgcccttc
      901 aagagaagcg gctcgtttta acagcatatg aacttcttgg ctcattctag cttcaaatcg
      961 tgcggttgca gttgccatat attttctcct tgaattattt agttttaatt gtacgtattt
     1021 ataccgtaca aatcaagcat gacaataaaa aaatccctta gattttcaaa cctaagggat
     1081 ttacctaccg atctttgtgt tttttaacgt gggctggccc tagtgccata caggcattat
     1141 aaagtattca tcaaatgaac atcaaactta taatggcttt ttttacgagt atcacaatca
     1201 acagagccga tacttggttg ttctttactt tggctaatcg ttgctcccgg ctcaataaat
     1261 tcttttgcat tgccatttgg ctgaacaaag ccgaccaaat acttcaatcc atcattttcc
     1321 agctctaatg catacgttgt aaaacctgaa cctctggcga taattgagcc gtcattagtg
     1381 actgcctttt taatcggatt tttgaaggtt gttttgtcat acgaatattc gtaatcattt
     1441 cctacccgtt taacagtaag cggttttccc tccgtgctgg tacaggaaaa tattacgttt
     1501 ttatctgctg caaatactgg ggtcgtgcag agtgtaagag cagtaattac taataattta
     1561 cgcataaatg taactcctta taaggttaat ttttaccaag aatataatca gcttcctgat
     1621 tccggcggcg tgcattgata ccgttattat caacctggcg atttctgaca gcagttgcca
     1681 aagcagtcat atcaccagtt tgagctgatc gtctagctgc gtcaagcaca ctttttgttt
     1741 tcgctagtga gccgtaatta tatgcataag acaccaaaac agcctgagcg ttcgccggta
     1801 atgcattcca atgtgaatca cctaactctt gtctcacatt gttcgcaaag atagcagttc
     1861 tgcgagctaa atcacgttca gcatcttctt tggtaatcac agtatccttg gtcactttaa
     1921 ctacggtacc gtctgcccgt gtaatcgtat cggaaccata tcctacacga tgggcattca
     1981 catcccattg agccttggag ataaagcctt caaaattacg cagtaaccct gttgcctgat
     2041 ccatcgttga accgctgatt tgtgcaagtg atgggaaatt tccgcctaca cctgaaccac
     2101 ctctatttgg tagagtcggg atgagagctg ctacattcgg aatatttggg atactcaatg
     2161 caatatttct acgagagagg ttaatattag agcctgtctc taaatcagtt aatgcaacat
     2221 taccgttttc aagtccttga acacgcttat ccagactatc ttgggataaa ctatgagcac
     2281 cagcaagagc ttcccgttcg gatttacttt gcagaatcac cgaagccgta ttgccggtga
     2341 tcattttttg catatccatt tccagtttta cttgggaaat ttcacggtca ataatattga
     2401 gtactctttc cagttctact tgagcatccg cattttgagc aacattcggc tctcgcatac
     2461 cagctaggat tgctcggcga gccagtagca ttttttccat tactttactg gtcgcaattt
     2521 cagcggataa acgttggctg agaagttcaa catctggatc atcacggagg ctttcaatca
     2581 agccacgagt gacaggtaca ctacttgagc ttaattcagc taatagctcg gatttcggca
     2641 catccatatt cagtacattt tctaatttaa caattgtacg aatatgttct tcttcaattt
     2701 ccggacttaa accaacaccg gctttcgaac tggttttagt accacaatcc tccttacagg
     2761 ttgaaattga ttgctcacca ataatcttag ttaagaagtc ggcagcagct tgaggtgtat
     2821 cccaagtctg acaaagatta ccttcacaat ttgaaaccgg gctgctatct aagacattcc
     2881 gcttattgag taaattaaat ccgcctttcg ctacatcacc aacgacatta attggatctt
     2941 ggttttgccc acctttacgt tgcccaccaa cccaaactct cccatctttc ccttttttat
     3001 cttctaggtc tttttgggct tgcttcgcat caggttgtgt cgaggtaatt tgcttatagc
     3061 tctctaaatc tgctgcttgc ccccattttc ctcctagtgt gtgatctgcc agaactttag
     3121 acatttcctc acaggaagtt tttagacggt tgaaatccag cttaccttgg tacataccat
     3181 ttgtcagcaa atcgtagagt tgagggttgg cccgttgcaa tatcattgca ggcatactgg
     3241 caaccgcacc tttagctgaa tcaatgacat tccccattaa atctttaaag ccatctttaa
     3301 ggccgtttaa atcattttta atagttgttt taatgtcaaa attgccacag gttaaattcg
     3361 ctttccaacc gatgcctaaa tcaacgactt tggtattacc acgactaggt ggagccatat
     3421 aacctgttcc tccgccgatt tggtaaaaga cacgatctga aataatcgaa tccgacttac
     3481 taaattccag tttatcagtg attgagtcag caaaagagat agaggaagta ctggaaaaaa
     3541 gtactgttat tactaataga tgtgttttct tgaatttaaa ttgtgatttc attgcaagac
     3601 ctcgttttag tgtgaccaat caatactgta aaggaatgtt tgccctcggc gtttacaaca
     3661 actataaggt cgccatagtg cccaagcata atcaccttga tttgaaatct gagaggaata
     3721 ggtgtctgtt tgccccccat ttggaaaaac agagcaggta tttgacattt gaggataaag
     3781 catttgccat ttatgtgttc tggcttgtcc ttcttcgaca ggtcctggtg gccattctcc
     3841 aacaccacct tttttcgctg ccgggaaata aatatgtggt tgagcagttc gggtcacgat
     3901 gtctgcaact ctctgcacaa tggttgcaga ggctttataa tcgtgagctt gtgttaccca
     3961 accggaacgt gggtaaatat ttccccagct atcgcctaca cggccaactt cccgcattcc
     4021 cggaatcaga gcttccggat aaaacatttc cggcatattt tcacgccaag caaagaaatc
     4081 taatccactt agaaaatagg gtataaatgg tgtggtttga cttttacaat agtagcctgt
     4141 acttcctaat aactgaccaa ttgctccttg gggatgcccg aaagcatctg catttttgaa
     4201 ggttagttga gagtattgac gttgcggaga acgatattct ccgcctttta cgccaggatt
     4261 gagagattgt aattctgtcc acggagtttg cccatcctgg ttataagatg aaacgaccat
     4321 ttccggtaaa taatgcttaa ttttggttga tgtacgaacc ttgcatccaa agtaggtgca
     4381 aaataaccag aagcacgttc cgacgacttt atagtctaaa cagcttgttg aggcacttga
     4441 tgcagcaatc gacgccgtat tcaatgatgc ttttacctgt tgaggatata aggttgttgg
     4501 tactgctaaa cttacagaga gaagtaccga aaaaaacgct cttttcattt tgattcaccg
     4561 tgttgttgaa cccatttatg ccattcatct agtgcttttt gtatatcagt cataccataa
     4621 atcacgctcg gagaggattg cagattatta aaaaacaccg caggcacttt tttaattcca
     4681 ttttgccatc cactgatcac cccttgataa gcatctttta attgctcttc ttgggctttc
     4741 caatctgctg aattaaagaa tgctcgtatt tctcgttcgg caatctcggg ggatttactg
     4801 agatttcgac tgatttcctc ttcaaaattc tctacttgat caagataata aacttgggag
     4861 gcaagttgtg aattttgaat ggggtacatc tttgttgtat agacaatgat attcggctgt
     4921 gcttgtgcca ctacagaaaa cactactgtt actatgctga aatatcctag aaatctactt
     4981 gctttcatca ctctctttcc gaataaataa gcagaaaatt agcgagattt ttacatagag
     5041 gataggaaaa actcttttca aaaaagagat atttttttga gcaaaaaatg gttgatttta
     5101 taaatggatt tcgtatgata tggaatatta agatgctgtg gaaacagccc ttaaaagtcg
     5161 cataactctt gagaagcggc ggctatccat cgaggtagtt gagttattta cccaaaatgt
     5221 ctcccagcat tctgaaaggg aacgctgcaa atttgcagga atgccaccta aacacctacc
     5281 ttccgaggga tatttcaatt cccttggaat tgcgatctcc tgaggaattt tatttattta
     5341 actttttata ggagaactcg ctatgagtca acaaaccgca ggatgggatg tgatcccatt
     5401 tgaatcgtca aaaaccgcaa aaccttctct tttttggaaa cttggcaata agctcggtaa
     5461 tattttcgtt tcggcgaaac ataaagctaa aagcaaatat gtcgaggtaa ccaaaggcat
     5521 caatcaacga attgctgaag aagatgaaat gcgagcctta gaattgcaaa tgtgcttaga
     5581 tgagcaagca gctgaatatg agcaggaaat ttctcgcatt aagcgtaaga tgtttttcag
     5641 ggctttattc gttgcgatta ttgccttttt atgtggtatg gctaccgttt ttggtactca
     5701 gctctcttta atctaattta actcacccat cggggaaatg ctatttccct tgtggtaata
     5761 catttcccct taattttgga gaaatgtatg tcagttcata tcaaactctc ctgtgttgga
     5821 gatattttgc caatcaaact caatgaatgg acaccggaac atcgagcctt acatcacgaa
     5881 attagccata aagctctcga agcctggcaa aatcgagata aaggcactca aagcgtgcga
     5941 tttatgcaac aaatgccagc ccgacaagga ctgcatttcc gctttctgag tgtatcggag
     6001 caagacggca agctcattgt cagtcgttca tagttggttc aatgtttggt aagctttttt
     6061 acaaaatgaa tcccaaagta ctagctaaaa tcggagaaga ttttagaaaa gctggttatc
     6121 ttatgggagc tggttttgtc ggaatggtaa tttccaatga tcagatttcc acatcggaag
     6181 gatttgttct ttttttgtgg ggattgtata tttggattat cgggcatatc gcactacatc
     6241 attctgagaa aatacttaat tcaaagcaag gagaaccgaa atgagtccgt actttttagg
     6301 tgctgctatt ggaacagcgt tattggcaac aatttttatt gttctagtaa cttgggcaaa
     6361 tcgagatatt ttgtttacaa aatcagataa gccacagtct aaatagcaaa actccattct
     6421 aacccatagg ggaacaagtt ccccgaggag cttgttctcc gttttacctt ccttttaccc
     6481 tatcgggaaa tactttcccg aattgggctt aagtgtttcc cattattttt tattttggag
     6541 aaacattatg tcagattcag tattaaacac ttattttaaa cacgctcttg tcaaagctgg
     6601 ttttccggat accttagata tggagtattc actcagccat tgtcaaggag atggagtcgc
     6661 attttatggt cggcttggta cagatgatct aatccagctt ttcaatcatc tcaatccaca
     6721 gaaacgtaaa cagaaaatgt ttgctaattt gctaacgcac attgaatctt gggaaaccca
     6781 cgatgatatt ggttttgaaa ttatacgaaa tcgctttggg tatcgttact cgcattggaa
     6841 tacgatggag ctttatgcta aaactgcaga tcagttagtg tttttcaatg atagtgatgc
     6901 tcgaagaaac tggtattttc ctacttcaaa agtggataaa tacaaagcat tgtgggacgg
     6961 atttatcgaa gatttggagc agtacatcaa agatatttcc cgtgaaatgg aatcggtcgg
     7021 ttatcaaatt attgaagcct cgccgtatca aacggaaacc gttttcaaat ttaatacgga
     7081 aaaatatcta gtagaactga aacgtaaacc ttctgagttt tattcagagc ttgttgattg
     7141 ggcatttggt gatatgtgtg attttgaagc agctatagag cagatttgtc aagggcaact
     7201 ctgttatgct gattttgaag caactgttac cgataagaaa agtgaaattc agttaggtct
     7261 tgctcgcttg agtggaataa gttactcgcc gaatgaccgt tcgtttagtg gttatcgaca
     7321 agagcttatt ggtgaggcga ttgcctctgc tcgccataat gcagcacgtt ttgcctactt
     7381 gcacgcctaa tttgcagatt tttactttaa tttgaccgct tgtagtggtt aattttcacc
     7441 ccgtggggaa atattttccc tattgggcaa aatgtttctc cactttttaa tttggagaaa
     7501 tgttatgaaa aatgaacaca atttagagca acttaaatat atttgggata accgcaacct
     7561 tgaaaataaa gtctatcaat atctgtttca catctgcatt gagccgatga aaaaaggaga
     7621 ttggaatcgt tatacgattt ggcttaattt tcacggtaac ttaatcaacg tgtcagacgt
     7681 tgtatatcga gttgtggaat ctcaaaagct caaagatgac atcaaaattt ggtatgacaa
     7741 gcaatactat tgccgtccaa ccaaagttga aacaacaggt tttatttcca tttttctgca
     7801 cctgttttat gagggcatag ctcgtatttt gctggaacag ggaatgatta ctgaaactga
     7861 agcagccgag aaaagttaca acttcagagc tgcaaattgt tttcaacgtt tgctttaatg
     7921 gaggtgagat aatggaacct cgcactgtta aagttattta cgatgatggc gattatttct
     7981 ttaccctaat caatgccgat gaacagacta ttcgtaatta ctacgctata ggcagttcaa
     8041 tcggttttga taacacaagg aaaattgtcg ctgttgaaat tttataaact acccatcggg
     8101 gaaacgactt ccccataggg cgatagtgtt tccccatttt tctacccgaa ctttggagaa
     8161 acacttatga accctcaatc aaacacacag cctaaatttg atttatatca agaagtgacc
     8221 gataaaatca tcgccgcttt agagcaagga acggctcctt ggcttaaacc ttgggatagc
     8281 gttattcctt atggtggtat gccgtgtaat gccatatccg gaagattgta ttccggaatc
     8341 aatgttttgt tactttggtt gtctgccgca gaacacggct atatccaacg caagtggatc
     8401 actgcaaaag cagctattga agccggtgga cacgttcgga aaggtgaaaa agcaaccctt
     8461 gcggtcagct atcgcccgta tgatcgagaa aaaaccgatg atgatggcaa ccttgtcctc
     8521 gatgacgatg gtaacccggt aatcgaacat ctcgcttcga ttaaaaagca ctgcctattc
     8581 aatattgaac agtgcgaaaa actgccacag gactggtatg aaacggttga aaccccagaa
     8641 cagctgactg acgaaaatca gtatgctatt tttgaggaga ttcgtttgat gttgaaaggt
     8701 atggatttgc aagtgatggt aaaaccaagc aatcaagctt actaccaccc acgcaatgac
     8761 catatcgtga tgccggaaat gaagcagttt cattcaccac aaaaattcca tagcgtgctg
     8821 ctacatgaaa tgactcacgc aacagggcat caatctcgcc ttgctcgtga tggtattgtt
     8881 tccggaaaag ctcgctttgg caataaagtt tatgcttttg aagaattggt cgctgaaatg
     8941 ggctgtgcat tcttgtgttc tcacctaggc tttgctgacg tgccacaaaa tgccagctac
     9001 attgaaagtt ggatcaaggt gttaagagag gataaacggg caattttcag ggcttcaggg
     9061 aaagctcggg aagcctgtca atatatgctg gatgccttaa acatcgcaca gctggctgat
     9121 aaatacgaag tatttgaaga agccgcttaa taaatttgcc gaaaggcatt tcccgtgggg
     9181 agatactctc ccttcggggc attgtgtctc cctttttatt cacttaacga gaggactcaa
     9241 tgctcaatct aaacttattt aaacgaccca ttgaaatttc gacacttgaa tcttggtcaa
     9301 aattctgtga tgatgttggt aaagtagctt ttgttgcagt acctgctatg ctgtatagta
     9361 attacgagtt ttggattaag ataagcagta taattggtct atttatagct acctatttct
     9421 gtttagttat cggtagaaat atacgccaat ttattgagaa ggagaaatca aaatgggatt
     9481 aatgatcggc atttggatta ttgcttttat tgcatttggg ttggccatat ggacaagcca
     9541 tattctaaaa aagcaagatg aacgccttaa acagctaaaa aatagctaac tttcaaaata
     9601 cggcacattt tgtgccgtat ttcttttgta ctggtggcaa atataagatt actatcatca
     9661 gtatattgta atatctcaac tcaacagcat tagtcaaaaa tcctccaaca tattcagaga
     9721 gtttttccta ctgagttctc ttattttttg gttcaatact ctccctttat aaattaaatt
     9781 tggttcagat aaaagccgct caatggcaaa aggataaagg agcccttacg ggcaagggta
     9841 atcccctaag ggggaataag
//
LOCUS       DANLUA010000091         9697 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLUA010000091 DANLUA010000000
VERSION     DANLUA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            63..1541
                     /gene="fruB"
                     /locus_tag="PXB44_001598"
     CDS             63..1541
                     /gene="fruB"
                     /locus_tag="PXB44_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL5282062.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1555..2499
                     /gene="fruK"
                     /locus_tag="PXB44_001599"
     CDS             1555..2499
                     /gene="fruK"
                     /locus_tag="PXB44_001599"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL5282063.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2525..4189
                     /locus_tag="PXB44_001600"
     CDS             2525..4189
                     /locus_tag="PXB44_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5282064.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4356..4874
                     /locus_tag="PXB44_001601"
     CDS             4356..4874
                     /locus_tag="PXB44_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL5282065.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5027..5890)
                     /gene="rarD"
                     /locus_tag="PXB44_001602"
     CDS             complement(5027..5890)
                     /gene="rarD"
                     /locus_tag="PXB44_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5282066.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            6068..6652
                     /locus_tag="PXB44_001603"
     CDS             6068..6652
                     /locus_tag="PXB44_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL5282067.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            6734..7192
                     /locus_tag="PXB44_001604"
     CDS             6734..7192
                     /locus_tag="PXB44_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5282068.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            7189..8046
                     /locus_tag="PXB44_001605"
     CDS             7189..8046
                     /locus_tag="PXB44_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5282069.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(8161..8640)
                     /gene="ispF"
                     /locus_tag="PXB44_001606"
     CDS             complement(8161..8640)
                     /gene="ispF"
                     /locus_tag="PXB44_001606"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL5282070.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(8642..9325)
                     /gene="ispD"
                     /locus_tag="PXB44_001607"
     CDS             complement(8642..9325)
                     /gene="ispD"
                     /locus_tag="PXB44_001607"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL5282071.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(9343..9621)
                     /gene="ftsB"
                     /locus_tag="PXB44_001608"
     CDS             complement(9343..9621)
                     /gene="ftsB"
                     /locus_tag="PXB44_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL5282072.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         2916 a         2088 c         2025 g         2668 t
ORIGIN      
        1 tatagagtaa actaaccata acattacagt ttaaaaatca actttaaatc caaggagcta
       61 aaatgcttaa tttatcggca aaaaacatcc gtttaaatgg ttcagcaagc aacaaagaag
      121 aagttatccg ttttgttgcc gccgaattag tcgccaaggg caatgtagca gccggctatg
      181 aagtcggtat gcttaatcgt gaaacccaaa cctctacttt tttaggtaac ggaattgcta
      241 ttccccacgg cacgcttgat acccgtgatt tagtcaaaga aaccggcgtg caaattatcc
      301 aacttccgca aggcgtggaa tggggcgaag gcaacactgc ttatgtagtt atcgggattg
      361 cggctaaatc ggacgaacac ttaaccttac tccgtcaact caccactgta ttaagtgatg
      421 aagaggctgc tgccacatta gcgaaaacgc aaaatttaga tgaatttatt gccgtattaa
      481 gcggtaagaa aaaccttcct gccttatctc aagagctgat ttctctgaat atcgaatctt
      541 caagccctat cactttatca gccattaacg ccgcaaaact acaagagcaa ggctatgttt
      601 cccaagcatt catcagcgat gtggttgcca acaaagcgtt acatttaggc aataatctat
      661 ttgtggtgga ttccgccaaa ggcaaccaag ctaacggtat cgctattgcc cgtaatacgc
      721 aaggtcaaac cctactaact gtggcagaaa ccgacaatgc tctacagcca ctattaactc
      781 aattattaaa accagaagta agaagccagt ttgctcattt ctctgcggag caaatgcttg
      841 gtttattaaa aggcgaaagc gtagcttcaa cccctgtaac caatgtcgaa gcgagtggca
      901 accaagtggt tggcacattc accattcgta acgacaacgg cttacacgcc cgcccggctg
      961 cggtgttagt acaaacggtg aaaccgttct cctcaaaaat cagcgttgaa aatttagacc
     1021 gcaacactgc accggcaagt gcgaaaagtg caatgaaagt agtagcacta ggggcaagcc
     1081 aagggcatcg tttacgcttt gtggcagagg gagaagacgc ccaacaagcg attgaggcat
     1141 tacaacaagc aattattaac ggcttaggcg aaagtgtttc attcgtaccg gcggtggcag
     1201 atacgattga aagcattgct gtttcagcaa ttcctgcgga agaaactgcg actgctgtcg
     1261 atgccaatac gaaagaagca acttttgtaa ttaaaaacga acacggttta cacgctcgcc
     1321 caagtgcggt gttggtgaat gaagtgaaaa aattcaacgc caaaattgaa gtgcaaaatg
     1381 ttgataaaaa ttcaccgctt gtaagtgcca aaagcttaat gaaaattgtt gcccttggcg
     1441 taaccaaagg cacgacgcta cgctttgtgg caagcggtga tgatgccgaa gtagcacttg
     1501 cggcaatcgg tgctgcgatt gaagcaggtt taggcgaata agcgaggaat cattatgcgt
     1561 attgcaaccg ttaccctcaa ccctgccttt gatttagtcg gcaaacttca acgtattgaa
     1621 atcggcgaag tgaacaccgt agaaaccctt ggtttatacc ctgccggcaa aggtattaac
     1681 gttgctaaag ttttagctga cttaggcaca aaactttcgg ttaccggctt tttaggcgaa
     1741 gaaaatcaag gcgatttcgt aaatgccttt aaacacaatg atttggctga tgaattttac
     1801 cgtattaacg gtaaaacccg cattaatgtc aaaatcactg aaaccgaggc tgatgtaact
     1861 gatctgaact ttctcggctt tgaaatttcg gaaagcgatt gggaaaaatt cgtcacatta
     1921 tctcatcagt gggaaagcaa atttgactta gttgcagtgt gtggatctct ccctcgtggc
     1981 gtaacgccgg aaaaatttgc ttcttggttg gaatctttac accgccaagg cttaaaagtg
     2041 gtgttagata ccagcaatgc ggctctcacc gccggcttaa ccgctcaccc ttggttagta
     2101 aaaccaaacc gccgagagct ggaagtgctg gtcgggcatt cactcaactc aatcgaagag
     2161 attattagtg cagctaaaca attacgttcc cgtggtattg aaaatgtaat tatatcaatg
     2221 ggcgaacaag gctctctttg gctaaatcac gaaggcgtgt tacaagcccg cccaccacgt
     2281 tgcgaaaatg tagttagcac tgtaggtgct ggtgattcaa tggttgccag cttgatctac
     2341 ggcttatctc aaggttggag caaagagcag accttgcgtt ttgcctcagc tacttctgct
     2401 ctagctgttg ttcaaagtaa cgttgggatt gcagaccgtg cggcgttaga cgatattatt
     2461 aaacaaaccg acattaccgt gatttcagaa aatcattaaa cctcaatcaa aggaacaaac
     2521 caatatgaat atttcttttg tatttcctca aacactaggc aacgcacgcc gtttcttagt
     2581 gaatgaaatg ttatctgccg ctgccaagca acaaggacac aatgttgtgg cggcaaacca
     2641 agctgatttt gtggtgttat tcgataacgc aattccacaa gaagctgccg gcaagcaagg
     2701 ggcaattttg gacttagaac aagcctttgc tcaacctgaa gccagtctca aacaagcggt
     2761 cgaaaatgca caaacttttg caaatgctac cgcaaccact gcggtttcag cggtttcatc
     2821 tggtgtgaaa aatatcgttg ctgttaccgc ttgcccaact ggcgtggctc atacttttat
     2881 gtctgcggaa gcaatcgaaa attatgccaa agcacagggt tggaacatca aggttgaaac
     2941 ccgtggtcaa gtaggagcag gaaatccaat ttccgccgaa gaagtcgcag cggcagattt
     3001 agtctttgta gctgccgata ttgatgtcga tttggaaaaa ttcaaaggca agccgatgta
     3061 tcgcacctca accggtttag cattgaagaa aaccactcaa gaatttgaaa aagcctttgc
     3121 tcaagcgaaa atttacgaag gtggtgtggc aaaagcggat aatgctgaag ctccaacttc
     3181 tgagaaaaaa gggttatata aacacttgat gaccggtgta tcacatatgt taccgctggt
     3241 ggtagcaggt ggcttgctca tcgctatttc ctttatgttc ggaattgaag cctttaaaga
     3301 tgaaaccatc gcaggcggct tgccgaaagc cttaatggat attggtggcg gtgcggcatt
     3361 ccacttaatg attgcggtat tcgcaggcta tgtagcgttc tcaattgcag accgtccggg
     3421 gttagctgtc ggtcttatcg gcggtatgct tgctaccacc gcaggagcag ggattttagg
     3481 tggtatcgta gccggtttct tagccggtta taccgtaaaa ttcttaaatg gtgcgattca
     3541 gctaccggca agtttaacct cacttaaacc tattttgatt ttaccactct taggctcagc
     3601 gattgtcggc ttactgatga tttatgtcat caacccaccg gtgaaagcat taatggatgc
     3661 cttagtggaa tggttaaaca caatggggca aaccaatgca attattttag gtattattct
     3721 cggtgcgatg atgtgtaccg atatgggtgg tccggtgaat aaagcggctt atacctttgg
     3781 tgtgggttta attgcttcac aacaatatat gccaatggca gcggtgatgg cggcaggtat
     3841 ggttccacca attggtatgg cgattgcgac cctgattgct cgtagcaaat tcaacaccaa
     3901 ccaacgtgat gcaggtaaag cttcattcgt gttaggttta tgctttattt cagaaggggc
     3961 attaccgttc gtggcggcag accctgtgcg tgtaattgcc agctcaatct taggcggtgc
     4021 aaccgccggt gcaatttcaa tggcactagg cattacccta caagccccac acggcggttt
     4081 attcgtgatt ccattcgttt cacaaccgtt aatgtactta gctgctattg ccatcggctc
     4141 attagtcacc ggcgtagtct atgccgctat caaacaaaaa gcagaataaa gcacaaacac
     4201 aaatacaaac aaaaacggag cgaaacgctc cgtttttata tcatattggt acttactgaa
     4261 ttctatttca tcgaaatatc aagtctaaaa tattattttt gcgatcgaga tcgaaaaaat
     4321 cattttcatc actttacgaa taatatcaac tcgttatgat tttgctacga ggagatacta
     4381 tgcagtttat tgaatttacc gatcctgata gtggaatttc ttatcaatac agtgaattta
     4441 ctattgctaa tgtcgcattt atcattaatt tctgctcaga tgccgatgtt atatcgactt
     4501 tgtccgcatt aggcaaagat atcacaaact atataaacac ctattcctgt tgcacaatca
     4561 aatttatggc gaaagagcat ctagaaaata gcggttctaa tattgacatt tatgcccccg
     4621 cagcgaatca tcagttcaaa cgtaaagaaa taattgcttt acaagaaacc cttgagcgtt
     4681 tattatttga gcattatgta cgttttacac cagaaagcta tctcttcatt gccgagagag
     4741 attcacttaa ccgaatgtat cagcgtatgt gtgtgccacg atgtgatttt atgcaatctt
     4801 tccaagtggt ttatcccttg ggcgttaatc aagattgttt cattttaatt acgccaaaag
     4861 gaaatctaaa atgaatcaac aacgtccagt ttccgttcgt ttaaagcaag aaggcttaat
     4921 cgcttttaag caagcctatc agcgagcaaa aaaagcaggt ctattgaaac cggtcaaata
     4981 atctttaaac cgcttgctct acaagcgatt tatttttcct taattatcac accttgctac
     5041 gaaaactatc ccaactcaat aatccaatcg aaaccagcaa acagaccatc aaaatatagg
     5101 aattggcatc gagtttttcg ccgatggcaa aagaaatcag caacatcacc aacggttcaa
     5161 catagcccag taaacctaat acattaatcg gtaataaatt gctcgagagg atataaagaa
     5221 taaaagcact gccacttacc agccccagca atccgaggta gaaataaatt tgcggatttt
     5281 ctgccactat tggcgtcatt tccacttggc tgagataata aagtgcaacg ggtaatacca
     5341 gtaacaactc cacaaaaaaa gtagctaacg tgttgatttg aaaataacgg cgaagggtaa
     5401 tataaagcgg ataacccaac ccagctaccc aagtcgccca cgaaatcgtg ctgttaaaaa
     5461 acaaactact gccaacaccg atgactgcga aaagtagaga aagccatttc aggcgggtaa
     5521 aatgttcttt aaacaccatt tttcctagag ccaccgccac aatcggtaaa agcaagtaac
     5581 caaacgacac ctgcaacgcc tgcccgtgat taggagccca taaaaacagc caaagctgca
     5641 cgccggtgtt gagagcaagg agcaaaatga ccaatacaag cggtggattt tgtcgaattt
     5701 tttgcaaaag atccgcaaaa gccgattgct gtttaaacaa tataatcgcc agcaaaataa
     5761 acggaatgac taccactatc cgaaagccga acatcgcctc tccctctaac gggcgaagca
     5821 atatcgcaaa atagtaaact gctccaaaca gcacattggc tgcaagcgaa gctaaaattc
     5881 ctttgagcat ttggctttcc tcttatcaac aatcaaacaa caagcggttg actttgaaca
     5941 aaaatttaca aatcttatct acgccccata gtatgaaaaa atttgtaagt aagctagcta
     6001 tattcgattt ttcccgatac aatgctccca atttttcact cttttattta tattatagga
     6061 cttcataatg aaaaagattt tttggatttt actgatcgcc atgcttgctg ttggcggtta
     6121 ttctttaaca aaatacaacg atttaatgcg tgcagaagaa gacatcaact ccgtatgggg
     6181 aaatgttgaa tcggcttacc aacgccgtgc ggatttaatt ccaaacttag tgaataccgt
     6241 aaaaggtcag gctaactttg agaaagaaac tttaacctct gtgattgaag ctcgtgcaaa
     6301 agcaactgcg gtaaccattg acccaagcaa tgcaactgaa gagcaaatgg ctaaattcca
     6361 agaagcccaa caaggtgtaa actctgctct ttcacgctta ttggtttcag ttgagaaata
     6421 ccctgaatta aaagcccacg aagcgttttt aaatctacaa gctcaattag aaggtacgga
     6481 aaaccgcatt aacgttgaac gcaataactt taacgaaaaa gtgaaagagt acaacaagca
     6541 agtgcgtgaa ttcccgacta aattagcggc aatgattatg ggctttaaag caaaaccgca
     6601 atttaaatct gaagcaggtt ctgataaagc accaaccgta aacttcaact aattttagtt
     6661 tcaatttatt catattaaaa gcaataagct ctttcaaatc cggtgcttat tgctttattt
     6721 ttaaggggaa caaatgggat tattttcttt tctctccaac aaattaccga tagatacgca
     6781 acgcattgaa caagcaattg ccgatttaga gcaaaaaacc tctgccgaat tgcgtgtggt
     6841 ggtggaacga aaagccaaaa tcggcaaaac cgacaacgcc gctatgttgc gagcaagtga
     6901 gcttttcgat gaattaaaaa tgcgtgaaac ggctgaacat aacggtgtgt tgatctacct
     6961 ttcattcaag cctcactacg ctgcgattat tggcgatgaa ggcattcatc aaaaagtcga
     7021 taacgatttt tggcaaaaaa tctatgccac aatgtgtttg gactgccaac aaggcgatta
     7081 cacccaagcc atttgtgatg caatcaaaat ggtggaagct ccgctcgctc accatttccc
     7141 ttatcaggct gatgataaaa acgagctttc aaacgaggtg gtagtcaaat gatgaaatcc
     7201 ctttgcaaat ttctatctat tttcaaccgc ttgttattgg tgtgcttggc tctgtttagc
     7261 ctaaatgcca cagcggtgac ctacccagag ccgcctaatc cgttttatta tattacggac
     7321 tacaccaaaa acaccttgag ccaacaagaa tggcggacat tagaagatgc cttaattgct
     7381 aactgtgcca agacaagttc gcaaattgcc gtggtgatta ttccaagcac ggaaggcgag
     7441 gcagtttcaa cctatgctca taccctgttt aacaaatggg gaatcggacg gagcaaaaat
     7501 aacgagaaca atggtatatt gttattaatt gccaaaaatg atcgcaaact ctttattgcc
     7561 accggacgtg ggcttgaagg agcgttacct gatgcgattg catcgagcat tatccgcaat
     7621 gacatcacgc cattcttcaa acaagagcaa tatgcagccg gtatcgccca tggcttatcc
     7681 gccattatgg cagccattaa cggggaatat gccccttatg caagttatgg cgaagaaatg
     7741 caagaatttg atgacataga cggcttactc tttttcttgt ttgccggcat tgtgattttc
     7801 ctaattttcc gcccacgagg aaacagtacc tatattagtc ccagctcaat ggatcaacta
     7861 ggacaaatac taagagaaag ccaacgcaga ggtgggggct ttggcggtgg tctcggaggt
     7921 ggctttgatc gaggctcagg tggatttggc ggaggcttcg gtggtggttt tggcggaagc
     7981 agaggcagtg gcgacagctt cggtggtggt tcatcaggcg gtggaggcgc tggaggaagc
     8041 tggtagtttc cgcccaattt aaaacaatac aagcggtcat atttctcaaa aaaattgcgt
     8101 atttttcgct aaacgcacaa aacgctaatg aaaataagac cgcttcctca cttctctaag
     8161 ctatgccctt tttagtaaca atgctacggc ttcacaagct atgccttcac ctcgcccggt
     8221 aaaccctaat ttttctgttg tagtggcttt tacattcact tgtgatatat cgcattgtag
     8281 atcgtctgcg atcacctgtc tcattttatc aatatgcggt cgcatcttcg gagcttgagc
     8341 aataatggtt acatcgacgt taccgacttg atagccctgt tcttgcactt gtcggtaagc
     8401 ttcaatcaac aaaccacgac tatcggcatt tttgtattgc atatcagtat cagggaataa
     8461 tttgccaata tcacctaaag ctgctgcgcc aagcaacgca tctgttaaag cgtgcagcgc
     8521 tacatctccg tcactatgcg caatgaagcc tgtatgataa ggcacttcaa ccccagcaat
     8581 catcagtggt ctatcttcgc caaaagcgtg aacatcaaaa ccgtgtccaa tacgtatcat
     8641 tttagttctc caaattatgt gtaaggtaaa attccgctaa ggctaaatct tcagggcgag
     8701 tgattttgat attatcactt ctacctgaaa ccaattgtgg ctggtagcct gcaagctcca
     8761 ttgcagatgc ttcgtctgtt acctgaagct tattggcaaa agcagaggat aatgcttgtt
     8821 ttaatgcttg agcaggaaaa aattgtgggg ttaaagcgtg ccataaggtt gagcgatctt
     8881 ctgtgtgtgc gattttcgtg ccgtttgctc ttttcatcgt atctaccgct ggaatggcga
     8941 gaatgccacc ttgcggatcg ttaatttgca aaagtttatc taaatctgac cgcttgaggc
     9001 aagggcgagc cgcatcgtga actaatgccc aagcattttc agtgttgatt tcctgtaagg
     9061 catttagcac tgaatctgct ctcgtttctc ctccaaaaac aagttggatc ttaggggatt
     9121 ttaggagttc caatgtggaa taaaaagggt cttttcttgc caccgctaca atcacttttt
     9181 caacatgagg gtgttcaaga aaaatagaca ctgtatgctc taagatagtc ttacccttta
     9241 attgtaaata ttgttttggt ttttctgctt gcatccggct accaactcct gacgccggaa
     9301 ttactgcaat aatttttcgc ttcataatat atcaattatg tattatttgt tttttggtac
     9361 aattcggtag aacatctcgt tctcttttac catttctcgt tcaagtcttg ctctctcttc
     9421 aagtgcatct acgccgttct ttaaatcgta aatttcggct tcaattagtt tattgcgtga
     9481 agtaagcttt gtgttttcta cttttaataa agcaatttct tcttgcactt cttgataatc
     9541 actccaacca ttttttccaa accaaaagct atattggaaa aagccgagta atagggcaaa
     9601 aaaagtaatt aataaacgca tttattttct cttatatgaa atagtaatga actaagcggg
     9661 ttgattttac cttgaatttg caaaaaattc taaaaat
//
LOCUS       DANLUA010000092         9695 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLUA010000092 DANLUA010000000
VERSION     DANLUA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            9..287
                     /locus_tag="PXB44_001609"
     CDS             9..287
                     /locus_tag="PXB44_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282073.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            complement(338..796)
                     /locus_tag="PXB44_001610"
     CDS             complement(338..796)
                     /locus_tag="PXB44_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5282074.1"
                     /translation="MSEPIEQVKTAFDSLLNNISKPRRRLMYQQIGRELARSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(789..1274)
                     /locus_tag="PXB44_001611"
     CDS             complement(789..1274)
                     /locus_tag="PXB44_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282075.1"
                     /translation="MIKPDRLRAVLTQTIDIFQANPEKLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRLPLTERVIVKEQDGHLTATHADEPNLADVSPFTRLTEYEVYLKDELIYQWAEPN
                     E"
     gene            complement(1271..1492)
                     /locus_tag="PXB44_001612"
     CDS             complement(1271..1492)
                     /locus_tag="PXB44_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL5282076.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            complement(1641..2096)
                     /locus_tag="PXB44_001613"
     CDS             complement(1641..2096)
                     /locus_tag="PXB44_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282077.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            complement(2093..2659)
                     /locus_tag="PXB44_001614"
     CDS             complement(2093..2659)
                     /locus_tag="PXB44_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5282078.1"
                     /translation="MSKSVKVGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            complement(2652..2858)
                     /locus_tag="PXB44_001615"
     CDS             complement(2652..2858)
                     /locus_tag="PXB44_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL5282079.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            complement(2864..3076)
                     /locus_tag="PXB44_001616"
     CDS             complement(2864..3076)
                     /locus_tag="PXB44_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5282080.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            complement(3073..3588)
                     /locus_tag="PXB44_001617"
     CDS             complement(3073..3588)
                     /locus_tag="PXB44_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL5282081.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            complement(3700..4389)
                     /locus_tag="PXB44_001618"
     CDS             complement(3700..4389)
                     /locus_tag="PXB44_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL5282082.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKKIQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            complement(4399..5427)
                     /locus_tag="PXB44_001619"
     CDS             complement(4399..5427)
                     /locus_tag="PXB44_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL5282083.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEELAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            complement(5441..6268)
                     /locus_tag="PXB44_001620"
     CDS             complement(5441..6268)
                     /locus_tag="PXB44_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL5282084.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQIEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            6418..8220
                     /locus_tag="PXB44_001621"
     CDS             6418..8220
                     /locus_tag="PXB44_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL5282085.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLESRLNLLIMKESKNNNDYKEMDALRRLLESTA
                     RIKKYSNGGGNEADLNPNIKNRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMMQ
                     RCLVDSMEVWRDYVFTDGYQRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAEQAAEIVAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            8229..9269
                     /locus_tag="PXB44_001622"
     CDS             8229..9269
                     /locus_tag="PXB44_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5282086.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGLEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMSDASQ
                     KQEDVENIRQQLKQSKGVGNFKNLFVHAPNGKKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
BASE COUNT         2425 a         2135 c         2142 g         2993 t
ORIGIN      
        1 gaaaatctat ggaaatctta tttatcttac ttgggttaat tggtttcggt gcattattgg
       61 caataggttt tacaaccttt gctgccattt ttggtgtagt tacaacagta tttttaagcc
      121 tgctttcatt attggcaaat atggttgggc tctctattat cgttattctg atctcacttt
      181 atgtattgtt accactcaat acttttattt tgatagcatt gattttttca gctattttag
      241 ggtttctttg ttggtatcat tcagatagac aaagcgttaa tcaataactc aagcggtcaa
      301 aattgaccgc ttttttgcaa aagtttctat tcatcattta gctaccagcc aaaatattaa
      361 tcatataatc ctcaatcatc tgcagatcgt catcagtaaa acctagcaac tcacggctgg
      421 catatttcac ttttaaatcc caatctttcc gcactcgtcc gattaagcct tgttggtgga
      481 ttcgggcgat gaccgcatca ccaccctcat aacctaagct gacaccttcg ctttcaaggc
      541 gtagccgcat aaaacgtggc tgggtaattt tgtcaaacat cttgcccgat ttaattttgc
      601 tttttacccc tttcttcggc tttttgcgtg gctcgtatgc cgagccgtct gggttttgtt
      661 gtgctttaat ccgtctgcgt tggcttcggg caagctctcg cccgatttgt tgatacatca
      721 accgtctgcg aggtttgctg atattattca gtaagctatc aaaggcggtt tttacttgtt
      781 cgatcggttc actcatttgg ctctgcccac tgataaatca gctcgtcttt gaggtaaacc
      841 tcatattcgg ttaaacgggt aaatggcgaa acatcggcaa ggttcggttc atcggcgtgg
      901 gttgcagtta ggtgtccgtc ttgctctttc acgatcaccc gctcggtaag cgggaggcgg
      961 atataaatat cgtagctctc gtgattgttg tggtcgattt caaattcgat tttgtcttgt
     1021 agttccgggt tttgcagtag ctcccattgt tcatttcgca caaaatttag cactggcaca
     1081 aacaacacgt cggggtgata cggaaaatcc accacgatta gctctaaatc gtagtggtat
     1141 tcaaacgaat ggctttggct ccctttgctt ttaattttgc ctttgtcata ttgcaaaatc
     1201 agcttttcgg ggttagcttg gaatatatca atggtttgtg tgagcacggc tcgcaagcga
     1261 tcaggtttaa tcatctgcgg tagccttttt gcttatgctc gtaaatggtt tggcactcaa
     1321 tgcaacgggt gcaaccaata accatttctc ggcgtttttc ggggattggc tctccgcaat
     1381 cctcacactc aaaaaggctg gtgcaagcgg tctgattttg cagaattttt gcaagtgccg
     1441 cctctctggc tttttcggct aattcgttgg ctcggtcgat ttggtctgac atagtcgtcc
     1501 ttattaaaaa aggcgatcac tcgccttggt tgattttgtt gtattgctcg atgcaatgct
     1561 cgaacgattc gatgatgact tggcatacct caatggtgtt gagtcttttg tctagtgctt
     1621 tgacaagatc accgttggtg ttaatctcgg ttgccggtgt ttggcactcc agccttttcg
     1681 gacagattat cgggttcgat ttgagggtgt gataccgcac gctttcgttg gttgagcagc
     1741 ttgccaacgc taggaggcac ggcagaatca gcccactttt tatgcttttc cagttcgtta
     1801 aggatttcat tacttcgttg ctccgcttgt ttcattttgg ctaacacttt ctgattgagg
     1861 tcgtccacct gtttttgata cgcctcaagc tgtatttggc tgttttcaag ctgattggct
     1921 aaattgcggt tatcggcttg aatcagttgg ttttgcgtgg tgcgtgcctg taagtctgct
     1981 ttcaggcttt cgttttggcg aaactgcacg aaattggcta atacgccaac tagcaccagc
     2041 acaatcatta gcgggctgaa atgcttattg gtcatttggc agagtttgac aatcatttaa
     2101 cctcctgccc gagacacagg gctttttctt tttctcggcg gattttcaaa cctcttagca
     2161 ctttacctcc cgagcggtta aaatcaggta tcctattaca catcgcttta aaatccttag
     2221 ccatagcata tttatggatt gttgtctgaa tgtatttacc attttcattt ggataaaatc
     2281 taaggttgta acatcccata ttaaatgctg ctgataccat cgccgaaaac tgattttcgt
     2341 tcattttgtc gccattaaaa taacgattga tacacctttc agctatttta agatctttta
     2401 tccaacgctc ggcaatctct ttatcggtgt atttgtgatt gacattaatt ttctcaccgc
     2461 ttgcctcggt tgagccaatg ccaaccgtga ttacatcggc agggcattta tacggatcac
     2521 gtctgcaacc ctccgcattg ccgataatgg ctaagcctgt ttcgctggtg cggatttcgg
     2581 agctgtaata ttgctttacc gtcccgataa tagctgcaac ggagcagaca aatacgccaa
     2641 cttttacact tttactcatt ctttttccct tggttttgta gctttagctc aaattctcgc
     2701 tctttcagtt tgtattcttt ggctcggtat gcccaattaa gtaataaggt taaaatagct
     2761 accagaatac tcactaccac cccccaatca gatagggtga ggctaccaaa aaagccaaaa
     2821 attgccccaa aataggaggc ttgttcagtt ttgtgcatat tttttaatcc caaagttgca
     2881 cgctggattt cgccaccgtt gcaaaacggc tctcaatatc cggcattgta actacagtgc
     2941 cgatcggcaa acgtggcaaa tgagccagtt cggggttata ttctaaggct tgctcgacta
     3001 agcctgccgt ttgcccgtag tgccgaaaga tcagctcgtc taaggtgtcg tcttggcgtg
     3061 caatcaaggt gttcatatca attcactaat cattcggttt tcgcccaaaa tatcacgcac
     3121 ggcaaagtgg taatctcgtt tcaaatcacc ggcggtactt tctagctctt cggctttgtc
     3181 gtggccgtct ttggtgctgt cataactgcg gtagcgttcg taaaggttcg ctactgcaag
     3241 gcaatagacc gctcgtttat atttgtaaac cagcacgctt tcgccgttga tttgctcatc
     3301 atcacaggct tgcaagttcg ctttttgtgc ttgctctgca ttgaggcgat aggcttttaa
     3361 gtcggcattg actgttgcca ttgcctcaat caccgcttgt tttagccgct cattaatgac
     3421 cgtgccatca atccgcattg catttcgcac ctccagtagc tcaatgttcg gaaaaaagcc
     3481 attattggtg atagtttcat cagattctgc ggtgggcttg atcgcatttt tgctggattc
     3541 atagccattc actttcggga tactaataac gtgattgcgt tgttccataa ggtttccgta
     3601 aggtaagggg tggggatttt aaaagggtgc aaaatgaata aatcaccctt taaaatccgc
     3661 cccttggctg gcgtggatac gctctatttc ggctttcgcc tactcattct gttcttctgc
     3721 tggtttcggc tcttcggctt gtttggcaag ttgcttttct aacttgtctc gcaagccttt
     3781 tgcaccgcat tgagggtcga gggctatcgc tctttgatag tgttcaaggg cggtttgtgg
     3841 ctctgcctct tggattagct cgcctagctc acgcagtaat ttggctcgca ctgggtcggg
     3901 catatcaaat tcagcggtga ggtcgtttgc ctgtttcagc acggctgcct caaacggctg
     3961 tttttggtct cgggctttct ttgccgcctc tgcaagctct tcaaccaagg cggttgccgg
     4021 tgtgcgttca aactgctccg gcattgccaa atcgtgcatt aaaacgtatt gagcaatcgc
     4081 aagggcggtt gggtaatcgc caatatcaat cgaccaaatc agcattgtgg tgataaccgt
     4141 gtcttgcaca ccggagcctt tggcaaggga gccttccacc caaggtcggt attgctcaaa
     4201 attgttgcgt ttaaactcaa tctttcgctc aatcgactgg attttcttta attggcgttg
     4261 atgggcttta agttgggcta aaactaagtc atagccttgt aagccttcaa ggttttctcg
     4321 gtttggggtt gcagactcac tttctgcggt tttccgcaaa aaatgagccc gagccggact
     4381 cattcgcata gttccacctt actctttatc tttgagtgta atgttttcga ttaatgccgc
     4441 acagccataa tcttcgacaa cgtagtcgat attttctgat tcgtaggttt caatacggtc
     4501 ttttttcggc acgttctcaa tatgcttgcg ggttgtcccc tcttgcacat agatggctaa
     4561 attttccagc ttagtgatta aaatcgcatt cgccgggaag aacggcacac gcaccgcttt
     4621 taagccccca atgcgttttt gtgagatcac aatatcagcg gcaagttctt cggttggtgc
     4681 gttttgctga ttcacaatcg ggaaatattt atccgcaagc aacttgcgac cggtaatcac
     4741 cactaaatcg gtgtcatcac gatgccattc atcaataagt tcttcggtgg cgtccattac
     4801 gagtgcgtca agattggcat actcttgacc tttgccgact aaaacttggt tgtctgtgct
     4861 ttcgccattc attacacgct ctttggcatc ttcacgcatt ttctgcaacc agccttttgc
     4921 cacatcttgc aataacgggt tgctgttgcg atcactggtt gccgcacgac ttgtgccgtt
     4981 aaagccgatc ataattaagt cacgtttgcg ttgttttgcc gcaacattcg ccactttttg
     5041 ctgaaaatca gggaatttcg cccacatatc caactgttta taattgatat gggtgtcaaa
     5101 attgatttgt tggcagtggt aggtttgttt taccaacttc gcaatagagg tggttttgcg
     5161 ttcaccgtca ccgctggtat cggtggtgct ggccacggtg tgagctgaat ctaaacctaa
     5221 ggtttcaccg gtttgctcat caacaaacac aaagctgata gattttaaga aatcagagct
     5281 ttcacgcacc ttttcataca gcttttgctg taccgatggt tgcactgtga atttaatgcc
     5341 ggttgccaat gcattaaacg gcaagttgtt taattcagct tggcgtgcaa ggtaggcgtt
     5401 atatttttca agggttaaat ctttcattcg tgatcctcaa ttaaaaatcg gtttgtacat
     5461 cacctgcttt cgcaccagtg gcttttgggc gtggggtgta attggcagac ggttcttgtt
     5521 ccagttttgc aaatttttcg gcaaattcgc ccgcttgttt ttgcaattcg gtaaattcga
     5581 tttgcaatgt gcttagttca gtttgcaagc ggtcgttttc tgtcgaaact ttgctaaatt
     5641 gctcagctaa cagttccatt gccttttcgt gttcggcaaa tttgccgtca ttgtcggttt
     5701 tggctttggt aaacatcgcc ttaaatttct ccagccacgc acctttttca ggttcagcag
     5761 taaattctaa attcgcctct actgcctcac tcacaaggtt ttccggcttt tgcttgcggt
     5821 ctgctagtgg gttatgggtt gcaccggcac aaaatgtgag gtattccgtg ccgaggcttg
     5881 ccggtgtatc agtcaccgcc aaaccaacta aatacgcctc gcctgtgtcc gcaaaattaa
     5941 ggtcaatttc cacagaggta taaactttct gcttgtcttt gactaacttc actaaggctt
     6001 cggttggggc gatttctgcc agtaactgta atttgccgtc ttcacgttct tctgttttaa
     6061 ccgctaacac atcgccatag ctgtaagaat gcgggtcatc tttctcaaac caacgccagc
     6121 ggaaatgctc aaggttaata cgagcaccat agttattttt cgggtcgtaa ttttcttcaa
     6181 tctgcgtgag ccattcacga ctaatttcgc gtccatctgt agttgctcct tccgttgcta
     6241 ccacaaacca tttggatttt gttgtcatct gtttttcctt gctctaaagg ggaggggtaa
     6301 aaatcggatt tatgatcttg ttttgtgccg gcggtttcaa tgtgtggtac gtgttaaatc
     6361 gggtttcaca gcctcaagca aatgcttaaa caaggtggat ttttgataat cgcacctatg
     6421 gaaaatgaaa atacaatcac tgaatacgag gtgttggctg ccaaccctct acacgataaa
     6481 cgagaggctc aatcaaagta ttgggcaggt tacaccgtga ccgaaatttc tcgtcagcta
     6541 aatattcccg tctctaccat tgcaagttgg aaaaagcgag agaaatggga cgaaatcagc
     6601 cccgttggac gtgtggaagc cacactggaa tcacggctga atttgctgat tatgaaagag
     6661 agcaaaaaca ataacgacta caaagaaatg gatgctttac gtcggttgtt ggaaagtaca
     6721 gcacgaatca aaaaatattc taacggcgga ggtaatgaag ccgacctcaa cccaaatatt
     6781 aaaaaccgta ataaaggcga ccgcaaaaag cccgagcaaa atgccatttc cgaagagcaa
     6841 gccgaactgt tgataaacgg ctttttagac ggaatgttcc attatcagaa aaaatggcac
     6901 gaagctgggc tgactcatcg aatccgcaat attcttaaaa gtcgtcaaat tggagcgact
     6961 tattattttg ctcacgaggc gttagttgat gcgttagtaa ccggtagaaa tcagattttt
     7021 atttccgcct cgaaaaaaca ggcattgcaa tttagagcct atattgtggc atacgccaaa
     7081 cgggttgccg atgtggagct aaaaggcgaa accattacct tgccgaacga gtctcaacta
     7141 attttcttgg gtacaaactc gaaaacggca cagtcttacc acggcaatct ctattttgat
     7201 gaaatctttt gggtaaaccg ctttgaggaa atccgcaaag tggcagcggg tatggcgagc
     7261 caaaaacaat atcggattac ttatttttct accccttcga gcattaccca ttccgcctat
     7321 ctgctttggt cgggcaagct gtttaaccgc aaacgtccga aagcggaaca ggtggaaatt
     7381 gatatttctc acgccaactt gaaaaacggc aaaaaatgcg gagacggcca atggcgacaa
     7441 atcgtgaata tctacgatgc cgaggcaggt ggctgtaacc tgtttgatat tgagcaactc
     7501 aaactggaaa actcgcccga tgagttcgag cagctcttta tgtgtgagtt tattgatgat
     7561 aaccagtctg tgttcaagtt cacgatgatg caacgttgcc ttgtggactc gatggaggtg
     7621 tggcgagatt atgtattcac ggacggctac caacgcccat tcggcaataa agaggtatgg
     7681 gttgggtatg acccgtctta caccggcgac cgctcggcgt tggtggtaat cgctccgccg
     7741 aaagtggacg gcgggaaatt ccgactatta gagtatcgca cttttaaagg ggctgatttt
     7801 gccgaacaag cggcagaaat tgtcgctatt tgtgcaaaat ataacgtgac cagactggcg
     7861 attgatacca cagggcttgg tgtcggtgtt tatgaaatcg tcaaaaaaga acgaccagat
     7921 gcggtggctc ttacttacaa cgtagagctg aaatccaaga tggtactaaa aggcttggat
     7981 attatcagca aaggacgatt tgaatttgac tcaatgcacg ctgttgaggt tggtgcgagt
     8041 tttatggcaa ttaaaaaaca gatcaccaac agcggacggc aagtgactta tgtggcagac
     8101 cgttcggaag aggcaagcca cgccgacctt gcgtgggctt gcttgcaggt atttattaat
     8161 gagccgtttg atgggaaact ggaagaaacg accggcacag tagatttttt agatgattag
     8221 gacaaaaaat gaaaaaacaa ttgcgcaaaa atttaaccca aaatgaccgc ttacaaccgc
     8281 aagcacaaac cgaaattttc agttttggtg acccgattcc ggtgttagac cgtgccgata
     8341 ttctcaatta tttggagtgt tcggcgatgt atgagaaatg gtataaccca ccgatgagct
     8401 ttgatggctt ggcgaaatcg ttaaggtcat caacacacca cgaaagtgcg atcatcacca
     8461 aagcaaatat tttgctttcc acttgcgagg ttgatagccg ttatttatca cgccgtgatt
     8521 tgtcatcgtt tgttaaagat tatttagtat ttggcaacgc ctattttgag gtggtgcgaa
     8581 atcgccttgg gcaggtacag cgaatcgaat cacctttggc aaaatatgtg cgaaagggtt
     8641 tagaggctgg tcaattttac tatgtgccac aacgattcga tcaccaagaa cacgaatttg
     8701 ccaaaggctc aatctatcac ttgcttgagc ctgatattaa tcaagatatt tacggcttgc
     8761 cacaatatct ttcggccctg caatctgcgt ggttaaatga gagtgctact ctgtttcgcc
     8821 gtaagtattt tttaaatgga gctcacgccg gctttgtgtt ttatatgagc gatgcgtcac
     8881 aaaaacaaga agatgttgaa aatattcgcc agcagctcaa gcagtcaaaa ggggtgggga
     8941 attttaaaaa cttgttcgtc cacgcaccaa acggcaagaa agatggaata cagattattc
     9001 cgattgctga tgtgtcggca aaagatgagt tttttaatat caagaacgtg agccgtgatg
     9061 atgtgttagc tgctcaccgt gtgccaccac aactgatggg gattatccca aataacaccg
     9121 gcggttttgg caacgtggct gatgctgccg aggtgttttt tatcaccgag attgagccat
     9181 tgcaagagcg attgaaagag tttaatcagt ggttaggtca agaggttatc aaatttaaac
     9241 cgtctaaatt gttacagcgg acacaataaa acagatcttt acaaactgac aatacagctc
     9301 ctagattggg gcttttttaa tatataaagc aaaagttttg cctgattaga ttatatattt
     9361 cggcaattta ttatatcaaa acaacaaata aaatacactt aaaaaataat ttttccttta
     9421 tttttcaacg acttcaccac ctccaacccc gcgcggtcta tccctcacct cgcccgcaca
     9481 cttaatttgt cgatttatac gcaaatgcaa aaaggcgatc cctacaagcc ccatataatc
     9541 gcaaagcgat cttaaaaact cgatcttttt acgcaatttt acgcacctca acgcaaaaat
     9601 attgcttgtc aggaaaaatc gttattcttt aattgcatat acatatactg cacattatag
     9661 attttaactt attgataaat aatgattatt ggtgc
//
LOCUS       DANLUA010000093         9359 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLUA010000093 DANLUA010000000
VERSION     DANLUA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            14..229
                     /locus_tag="PXB44_001623"
     CDS             14..229
                     /locus_tag="PXB44_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL5282087.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(288..1445)
                     /locus_tag="PXB44_001624"
     CDS             complement(288..1445)
                     /locus_tag="PXB44_001624"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL5282088.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1567..3633)
                     /gene="prlC"
                     /locus_tag="PXB44_001625"
     CDS             complement(1567..3633)
                     /gene="prlC"
                     /locus_tag="PXB44_001625"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL5282089.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3764..4504)
                     /gene="pflA"
                     /locus_tag="PXB44_001626"
     CDS             complement(3764..4504)
                     /gene="pflA"
                     /locus_tag="PXB44_001626"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL5282090.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4753..5778)
                     /locus_tag="PXB44_001627"
     CDS             complement(4753..5778)
                     /locus_tag="PXB44_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5282091.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6013..8337)
                     /gene="pflB"
                     /locus_tag="PXB44_001628"
     CDS             complement(6013..8337)
                     /gene="pflB"
                     /locus_tag="PXB44_001628"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL5282092.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8427..9251)
                     /gene="focA"
                     /locus_tag="PXB44_001629"
     CDS             complement(8427..9251)
                     /gene="focA"
                     /locus_tag="PXB44_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL5282093.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
BASE COUNT         2634 a         2110 c         1844 g         2771 t
ORIGIN      
        1 ttaggagtta aatatgtttc cagaatttcg tgatctcata acccaattaa agcaaaacaa
       61 ttcacatttt gctcgcttat ttgataaaca taatgagcta gaccagcgta ttaagaatat
      121 ggaggcaaat attgaactgg caacacatga agaagttgag gcactaaaaa aagaaaaatt
      181 gcatttaaaa gaccaacttt atgcaattct aaaacaagaa tcggcttaac ctaaagtaaa
      241 agctcagaaa atattctgag ctttaatttc ggtcatatta gacaatatta cagttttacc
      301 ggctcaataa tttcgcttgg gtagcaaccc aacactttga ggtagctggt aacttgctcc
      361 aattccgcca atgctttttg ggtgttctcg gaatgaatat ttgcctccag ctccacataa
      421 aacatctctt cccaaggttt gccgtaaatc gggcgggatt ccagtttcat catacgaata
      481 tgatgatttt tgaataccat taacgcatcg accaatgcac ccgcctgttg cgtggtggtc
      541 attaacagta acgttttggt ttgtacctgt ggcgaaacct gcacagcctc tttggcaaca
      601 acaataaaac gggtgatatt gtgagcttga ttagcaatat cggttttgat attcgtcaag
      661 ccatacaatt ttccgccatc ttcattgccc aaggcaacaa tattcggctt attcaagcga
      721 gccaccattt gcattgcgtg cgagctgctt tcgcaatatt tgatatgtac tttatctagc
      781 ccggctaaaa attggctaca ttgctgggca ggctgagggt gggtgtaaat ggtatccacc
      841 tccgccaaat caaccgtgcc gtttgccaat acacaatgtt tgattgggta cgccaattca
      901 cccactaagg ttaaatcggt atgctgcaat aaatcataca cttcattgat cgaacctgaa
      961 gtcgtgtttt caagcggcag tacgccaaaa tcggcttcac caaggctgac tttctcaaat
     1021 acttcattaa aagaatggca gcttaattcc gccaatgtgc cttgatattt tttggcaaac
     1081 tgtcgagatg ccatattaga ataagagcct cgcattccca aaaacgcaat cgaaacctgc
     1141 tcttcttttt gtgcattcaa tttattttgc agataatttt gctgagtcag cactgaatct
     1201 tcaataatac gttggaaaat ttgggtcaca tattgcggat caagctggta attctccgct
     1261 tcggcaaaat ttaccagttc ttgaagcaaa atcttttccc gttctaaatc tctcaagggt
     1321 ttttgtgtga tttctttgct ccgcaccaca tcaaaggcta agcggtggcg ttctgccagt
     1381 agctttaata aattgcggtc taagttggtt atttgggttc gaatatcggc taaatctaaa
     1441 gacattattt cactctctgt caggctaaat gctgaaaatt ataacgagtt gcaaaagaaa
     1501 gaaaagacaa gcggtcaaat ttacaaaaaa atttgcaaat ctaaccgctt gtaaaacgtg
     1561 tttactttat tgagtgcaat caacaggctc ggcattacct acaatgcctt tgtgtctgag
     1621 caaggcatca agggttggct tacgcccacg gaagcgttcg aacagcacca ttggctcttc
     1681 tgaaccgcct cgggttaaga tattatctaa gaaagactgc cccgtttcag ggttgaaaat
     1741 cccctcttcc tcaaagcgtg aataggcatc tgctgaaagc acttccgccc ataaataact
     1801 gtagtagccc gccgcatagc cgcctgcaaa aatatgcgag aaactgtgtg gcgttctcgc
     1861 ccactctacg cctttggcaa cagcgacttg gtctttcacc tctttaagta aggctaacac
     1921 ttgtccttgt ttgctcgggt catattcggt gtgtaggcgg aaatcaaaca agccaaattc
     1981 taattggcga agcacaaaca ttgccgcttg gaagtttttc gccgccaata attgctctaa
     2041 tttcgctttt ggtaacggct cgccggtttc ataatgccct gaaataaagg ctaatgcctc
     2101 ttcttcccag caccagttct ctaagaattg gcttggcagc tccactgcgt cccacggcac
     2161 accgttaatg ccggcgacat cgcccacgtc aatttcagtt agcatatggt gaatgccgtg
     2221 tccaaactcg tggaacaaag ttgtcacttc atcgtgggta aacaatgccg gtttatcgcc
     2281 aatcggttta ttgaagttac aggttaaata tgccaccggt ttttgtaatg tgccgtcagc
     2341 caagcgtttt tggttgatgc aatcatccat ccacgcccca ccacgtttgt tttcacgagc
     2401 gtataaatct aagtagaacg agccacgcaa gcggtcattt ttatcaaaaa tattgaaaaa
     2461 acgtacatct ttgtgccata catccacatc tttttgttct tccacacgca taccgaaaat
     2521 gcgtttaatt acttcaaata agcctgataa tacgcgattt tccgggaaat acgggcgaag
     2581 ttcttcatca ttgaccgaat acaatgcctg tttttgtttt tcgctgtaga acggaatatc
     2641 ccataaatcc aaatgttcaa tgccgaaatg ggctttagca aattttttca aatcgcataa
     2701 ttcatttttg ccttgagctt tcgagcgagc ggctaaatcg ttcaagaaat ctaatacctg
     2761 ttgtggattt tctgccattt tggtggctaa tgaataatca gaataagtct taaagccgag
     2821 aagttgggct ttttcgtgac gcagttttaa gatctcatca atcaccgcac tgttatccca
     2881 tttgcctgca ttcgggcctt gatcagaagc acgagtcaca taagcacggt acatttcctc
     2941 acgcagtgct ttgttttcac aatacgtcat caccggcaaa tagcttggga tttcaagagt
     3001 gaagcgatag cctttcttac ctttgctttc tgcggataat tttgccgctt ctaatgccga
     3061 ttccggcaag cctttaagct gtgcttcatc ggtaattaca atatcccaac ccattgttgc
     3121 atcaagtacg ttgttgctaa attgcgagtt gagttccgac aagcgtgcag aaatttcccc
     3181 gtagcgtttt tgtttctctg ccggcagtga aatgcctgat aattcaaaat cttgtaagct
     3241 gttttcaatc gcctttttct gtgcaaccga ataagtttca aactcagggc tgttttttaa
     3301 cttcacatag ccttgatata acccttgatg ttgccccgcc caagtgctgt attccgataa
     3361 taacggcaag cacgcttggt aagcctcacg caactctggg gaatttttca ccgcattcaa
     3421 atgccctacc ggtgaccacg cacgggagaa tttatcctct gccattgctt gtggcatata
     3481 gaaattttcc cacgtcgggt tttcaatttg tgcgactttt tcaacagttt cacggcattc
     3541 tttgatcacc gcttctaccg ccggtttgat atgttcaggt ttgatttgtg agaacgcagg
     3601 caagcctgtg taatttaaaa gagggttaga cattattttt tccttttatg gtcaaaatga
     3661 aaattcgtat caagataata cacaaagtga gagagaaaag aaaacaagcg gtcatatttc
     3721 aataattttt tgcaaaaaaa tcacaaaata tgaccgcttg tctttagaat ttgactgtat
     3781 gcccgtaact ttcaatgatt ttttggatac gctctaaatc ctctttaggc ggtgacatta
     3841 ccccttccag ctcatattta tagcctaagg tttcccattt gtgagcccct aaacgatgat
     3901 aaggcagaag ttccactttc tcaatgttat ccattccttg aataaactgc cctaaacgat
     3961 gggcagaatc atcgtcatca gtataacccg gcacgaccac atagcgaacc caagtcggtt
     4021 tatttaattt gtgcagataa cgggcgaaat caagggtacg cttattcgac accccgacta
     4081 aatcttggtg aacttcatca ttgagctgtt ttaaatccag caagaccaaa tcggtcactt
     4141 caagcatttc atccaccacc gggctataat ggcgaacaaa accattggta tctaagcagg
     4201 tattaatgcc ttcagcctta caggcacgaa accaatcccg cacaaactcc atttgcaaca
     4261 ccgcttcccc acccgaagct gttacaccac cgccggttgc tttcataaaa tgcttataag
     4321 tcgtgacttc tttcatcagc tcttcaaccg taatttcctt accaccatca agatcccaag
     4381 tatcacgatt atggcaatat ttacaccgca tcaaacagcc ttgcaagaac agaataaaac
     4441 gaatccccgg tccatctacc gtaccgcagg attcaaaaga atggatacgt ccaacaacag
     4501 acatttgtca cctcaaccct caaaaaattt tgagaaattg tagcaaattt ttatgttgtt
     4561 cgcagaaata aatcacaccg gtatatttta agaaaggaga aagtcatact attttttgcc
     4621 tatttcagga ataaactttt attaatctat tgactccatc aacatatcaa ccaaatatgt
     4681 ttaaattttt gcatttttta aagctatttt gaccgcttac aagcagtcgg tttttctgaa
     4741 aactttgcaa acctagccgg cgtggtattt ggcggcgacc tcttttacca gtaagtcgaa
     4801 atcgctttcg taggtgagat cctgcttgac tcggtatttt tcaaattcgc tgagggcgaa
     4861 agatttagca atttcagcgg taactttgcc ggcatcttgt aagatttctc ggtcggtggc
     4921 ggcaagaaag cggttaaggc gggtttccca gtctgccatt gtcatcggaa tttggcgttc
     4981 tgccatatct tccgccatat ccaaataggc agagaccagc cgttgtagtt gtgctaattc
     5041 attatccgat aaataatttt tggcaaccac cacatcaaag gggtaaattt tgccttgcgg
     5101 agcgtctttc caattcgtta gccccatatt cggcttttca gcgttggctc gtgccataat
     5161 cagctcggcg gcggtatgcc cgtgaattgc ccaatggagc ttgttctgca cggtggcaaa
     5221 aaagcgtttg gtggcggttg cggtgcggtc gtagtccacg gcggtagcgt aaatgtcggt
     5281 gattttttgg tagaacttgc gttcggataa ccgaatttcc cgaatacgag caagctgttc
     5341 ttcaaagtat tttttgccca aaatcgtgcc gtcatttttc agccgttcat cgtccatcgc
     5401 aaagccttta atcgcaaagc tatgcacgat ttcggttgcc catttgcgaa actgcaccgc
     5461 tcgctcggaa ttgactttat aacccaccgc aataattgcc gacagattat aatgcttggt
     5521 gtggtagttt ttgccgtctt gggcagttat ccaaaaaaat tggataactg aattttcagc
     5581 taactcattg tcactaaaga tttttttgag atgatagcta atggttcgta catcaacatc
     5641 atacaacacc gccatcattt tttggctaag ccatacattt tcatcggcgt aaacggcatc
     5701 aacttggctt tcgccggtag ctgtaataaa agtgaggtat tccgccacag aggaacgaat
     5761 taaatcttgc ttagacatct ctttctccca tatagaacac tgtaattata aacaatatta
     5821 cgaaagctgt gtaaatgaat agccaatttt tcgagcgaga tcacaaaaaa accgcttgcg
     5881 gcataaatgc tacaagcggt tgattattac tcacaaaatt gtttccaatt ttgttcgctc
     5941 ctacggagcc gtcggcaaag ccaacgttca aaacgcaatg cgttttgtgg ctaaaagttt
     6001 tgcaaattgt aattacatac tttcagtgaa ggtgcgggta attacgtcca tttgttgctc
     6061 tttggttaaa gagttgaaac gtaccgcata gcctgaaaca cgaatcgtta attgcggata
     6121 tttttcaggg tgttctaccg catctaataa tgtatcacgg tttaacacgt tcacatttaa
     6181 gtgctgaccg ccttctacag ttgcttcgtg gtggaagtag ccgtccatta agcctgcaag
     6241 gttacgtttt tgggcttcgt aatctttacc taacgcattt ggtacgatag agaaagtata
     6301 agaaatacca tctttcgcat aggcaaacgg taatttcgcc acagaagtta atgaagctac
     6361 cgcacctttc tggtcacgac cgtgcattgg gttcgcaccc ggtccgaatg gagcacctga
     6421 gcgacgaccg tccggtgtat taccggtttt cttaccatat accacgttag aagtgatagt
     6481 aagcacagac tgagtcggcg ttgcattgcg gtatgttttg agtttttgaa ttttcttcat
     6541 aaaacgttca actaaatcaa ctgcaatgtc gtccacacgg ttatcgttgt taccgaattg
     6601 cggatattca ccctcaattt caaagtcggt tgctacgttt ttcgctacac caaccacttc
     6661 gccagctttg tttttgattt caatatcggt acggattggt tttactttcg catatttaat
     6721 ggctgaaagt gagtctgccg ctactgaaag ccctgcgata ccacacgcca ttgtgcggaa
     6781 cacatcacgg tcgtgaagag ccattaatgc cgcttcgtat gcatatttat cgtgcataaa
     6841 gtggataatg ttcagtgctg tcacatattg ggttgcaagc caatccataa agctgtctaa
     6901 gcgggtcatt acatcatcgt aatctaagta ttcacttgta attgcttcag ttttaggccc
     6961 tacttggtcg cctgatttct catcaacacc gccattgatt gcgtataaca aggttttcgc
     7021 taagtttgca cgagcgccga agaactgcat cattttaccc acgatcattg gcgatacgca
     7081 gcacgcaatc gcatagtcat cgttttggaa atccggacgc attaagtcat cgttttcata
     7141 ttgaactgat gaagtatcaa tagacacttt cgcacagtag cgtttaaatg cttccggtaa
     7201 ttgttcagac caaagaatag ttaagtttgg ctccggtgac ggccccattg tgtaaagggt
     7261 gtgtaaaata cggaagctgt ttttggttac taaggtacga ccgtctaaac ccataccggc
     7321 taaggtttcg gttgcccaca ttgggtcgcc tgagaataat tgatcgtatt ccggagtacg
     7381 caagaaacgc accatacgca atttcattac taaatggtcg attaactctt gggcttcttg
     7441 ctcggtaatt ttgccggctt ttaagtcacg ttcgatataa atatctaaga atgaagacac
     7501 acgaccgaaa gacatcgccg caccgttttg cgatttaacc gctgcaaggt aagcgaaata
     7561 tgtccattgc accgcttctt gtgcattggt tgcaggacct gaaatatcaa aaccgtagct
     7621 tgcagccatc tctttcattt tgcctaaagc acggtgttgc tcggcaattt cttcacgcag
     7681 ttgaatcgtg gcttgaatat cttcgccgga ttctaaacga gcttgtaatg aggcaaactg
     7741 gcgttgtttg tctttcatta agaagtctgc accataaagt gccatacggc ggtagtcacc
     7801 gataatacga ccacgaccat aagcatccgg caaaccggta attacacctg atttacggca
     7861 acgtaaaata tccggagtgt aaacatcgaa taccccttgg ttatgggttt tacggtattc
     7921 ggtaaagatt tgttctactt caggtttaag ctcacggcga taaactttgc acgaaccttt
     7981 caccatattg ataccaccaa acggcataat cgcacgtttt aatggagcat ctgtttgcag
     8041 accaacgatt ttttctaacg atttatcaat ataacccggt gcgtgtgagg taatggttga
     8101 tggcgtgtcg cagtcaatat catacggttc gtgtgttttg ttttctactt tgatcatttc
     8161 catcacatct gcccacaatt tgctggttgc aggtgttact tcagctagga aagactcatc
     8221 accctcataa ggtgtgtagt ttttttggat aaaatcacgt acatttactt cagtttgcca
     8281 ctcgcctgcg gcaaaacctt cccacgcttt ttgttgagcg tctgttagtt gagtcatttt
     8341 aagtcctctg tgttagtaag ttaaaacggg aaagcggtga ttttttgcaa aaaatttgca
     8401 aaatttgacc gcttgaatct gaaatcttaa tgctttctaa tgtataagaa ccactgcatt
     8461 aaaccaatga aaaacacgcc accgacaata ttacccagcg ttgccggaat taagttctta
     8521 aacacaaaat gagatacatc taaatcggca aattgcgttg ggtcaacatt aattgctgtc
     8581 caaaattcag ggctggcaaa gtgcgaaatc gcaatcccca tcggaatcat aaacatattt
     8641 gccacacagt gttcaaagcc ggaagccaca aacattgcaa tcggtaacat cataataaag
     8701 gctttatcgg ttaggctttt acctgcataa gacatccaca ccgcaatgca caccataatg
     8761 ttacagaaaa tgcctaaaca gaaagcctca aaccattggt ggtgaatttt gtgctgtgcg
     8821 gtatttaaaa taaccaaacc ccattgccct ttggctgcca taatctgacc actaaaccaa
     8881 atcaacaata caatggaaat cgccccaaca aagttaccga aatacaccac tacccagttt
     8941 ttaaacatct gtaaccagtt aattctttgg ctggctctgg ctacaatcgt aagtgttgat
     9001 gaggtaaaaa gttctgcccc aaacaccaca cacataatca cgccgagtga aaataccaaa
     9061 ccaccaatca gtttagccat accccaaggc atttggctcg cacccacttg ggttgtggta
     9121 taaaatacaa atgctattgc aataaaaccg cccgcagtaa tggcagagag aaaggaatat
     9181 aattgatttt ttgttgcttt ataaaccgca ccatcttctg cgatttttgc catttcagca
     9241 ggagaaaaca tcagacaacc ttaacaatga gaattgaaaa accgatatat tatgtagaaa
     9301 ttatagacct cttgagataa tcttcaaata aaaatattac acaatcagag gtatttcga
//
LOCUS       DANLUA010000094         9347 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLUA010000094 DANLUA010000000
VERSION     DANLUA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            363..1322
                     /locus_tag="PXB44_001630"
     CDS             363..1322
                     /locus_tag="PXB44_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL5282094.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            1315..3066
                     /locus_tag="PXB44_001631"
     CDS             1315..3066
                     /locus_tag="PXB44_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282095.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            3069..4034
                     /locus_tag="PXB44_001632"
     CDS             3069..4034
                     /locus_tag="PXB44_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5282096.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            4021..4914
                     /locus_tag="PXB44_001633"
     CDS             4021..4914
                     /locus_tag="PXB44_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5282097.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            4985..6037
                     /locus_tag="PXB44_001634"
     CDS             4985..6037
                     /locus_tag="PXB44_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5282098.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(6046..6276)
                     /locus_tag="PXB44_001635"
     CDS             complement(6046..6276)
                     /locus_tag="PXB44_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL5282099.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            6406..7488
                     /gene="leuB"
                     /locus_tag="PXB44_001636"
     CDS             6406..7488
                     /gene="leuB"
                     /locus_tag="PXB44_001636"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL5282100.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(7575..7970)
                     /gene="msrB"
                     /locus_tag="PXB44_001637"
     CDS             complement(7575..7970)
                     /gene="msrB"
                     /locus_tag="PXB44_001637"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL5282101.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            8195..9199
                     /gene="gap"
                     /locus_tag="PXB44_001638"
     CDS             8195..9199
                     /gene="gap"
                     /locus_tag="PXB44_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL5282102.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYKG
                     "
BASE COUNT         2875 a         1658 c         1981 g         2833 t
ORIGIN      
        1 gtctaataca tctgcagctt cttgaagctc agctttaatt tgttctaaat acatcatttg
       61 ctcctttgtt tgcaaaacgc ttacctttaa acatcggctt taccaatggg ggtagcgagt
      121 aaaaaattcg ttttttatga ccgcttgagg cataaaaagt gcaataagtt tagaagatct
      181 atgctgtatt gtaaacctaa taacttaaat aatctgaata gggttgtaaa caaaagtttc
      241 caaaagagaa aaatagcggt atgatcgcct cacaatccct ctcgcattgt taactctagt
      301 agctaatatc aggctttaag cgtattaaac aatgcacaaa tttgctattt ataggagact
      361 atatggtcgt aaatccagaa tcttttgaac agataattgg acaatctgta aagataaaag
      421 aggttgttga acaagcaaaa aaatttgcca accttgatgc accattattg attcaaggtg
      481 agacgggaac aggaaaagat ttatttgcca aatcttgtca ccattttgga tctcgtcgaa
      541 tgcaaaaatt tattgccgtg aactgtgccg gtttaccggc tgaagaagca gaatcggaaa
      601 tgttcggaca tcgtggcaat ggcgttgagt ccgtcggttt ctttgaatat gctcacggtg
      661 gtacagtatt attagactct atttctgagt tatcgctgga aatgcaagcc aaattattgc
      721 gctttttaaa tgatggctct tttcgtcgag tgggcgaaga tcaagagatt caagtcaatg
      781 ttagggtgat ctgtacttca ttacagcctt tgtcattatt agttgaaaaa ggcaaagtaa
      841 gagaagatct gtttcatcgt ttaaatgttc tcactttaaa tttaccacct ttgcgagatc
      901 gaaaagagga tattccatta ttagctgatt actttatcgc ccaaattagc aatcagttgg
      961 gggtgagcaa attagagtat gataatgttt ttttgcaagc attgcagaat tatcactggc
     1021 gaggcaactt aagagagctt tataacgcac tttatcgagc ttgtacactt tcgcatagct
     1081 accgtttagc ggtaaaagat cttcatttac ccagagatat ggaggcgatg attgaagtac
     1141 gtgaggaggc gacacttgag gaaatgatga gccagtatga agcccaatta ttgcaccaat
     1201 tttatgcaga atatccgagt acccgaaaat tagcacaacg gctcggactt tcacatactg
     1261 cggtggcaaa caaattgcgt caatatgggt taacaaaaaa tggaaacagt aaagatgaat
     1321 aaactagcgg tcattttttc agtatgtttt gcaaatttag ggctaaattc gaccgcttac
     1381 gcagcgccaa gtattcctca agagctactt gaaaatagcc ttgtgtattg cactacttca
     1441 tcgggattct cttttaaccc acaaaaatcc gatgtgagtt cgaatatgaa tgtggtaaca
     1501 gagcagatct acgataaatt agttgagttt aatccgaaaa aaaatcgact agagcctgct
     1561 ttagctgaaa gttattcaat cagcgacgat ggtttagtga ttacattacg tttaagaaaa
     1621 aatgtcgctt ttcatacaac tccatggttt actccgagtc gtaaattaaa cgcagaagat
     1681 gtggtgtttt cgctaaaacg aatgattggt aaagaaagtg atttacctga agtgaaaagt
     1741 gagcctgaaa gtgtattaca ccaccagcga caaagctata tttaccaaaa aacagcgggg
     1801 cagatttatt ttccttattt tgaaagtgtt gatttagcga ataaaattgc cagcattcat
     1861 tctccgtcaa atgacattgt aaaaattgaa ttagtccgtc ctgattcagc attgttggcg
     1921 catttagcca gccaatatgc agttattctg tctaaagaat atgctttaca gctcaatgca
     1981 gatgataact tagttcaact ggatttatta ccggtaggaa caggcgtata tcaacttgaa
     2041 agttatgaaa ataatgatta tgtgcgttta aaaccaaata tgaattactg gggcaaaaaa
     2101 gcatatattc aaaatatggt cgtagatttt tccacagaag cgaccggcag gatggcaaaa
     2161 tttttaaata atgaatgtga tgtggttgct tttccggaac ctagccaatt atcagtgtta
     2221 aataatcggc agcttattag tagtaaaggt gctaatttgg tttatttagc attcaatatg
     2281 caacggggta ttgggcagga tttggcatta cgccaacgta ttgcacgcgg cataaatcgc
     2341 aaacgtattg cagagaaatt attttatggt acaggtgaag tagcggataa cctattacca
     2401 acagctttgt ggtcgaaaaa aaataatgga agctaccctt atcaaccaca ccaaaattca
     2461 ggtgagcaag cggttgaaat gcatgacagt attataaatc ggtctttaga tagcaaaaca
     2521 gagaccgaag aagtaaacca aaaacagcgc ttaaaactgt gggtaattga tgaaaaacga
     2581 gtttataatc aacacccatt aaaaatggca gaaatgatac gtgctgattt agctacgcaa
     2641 aatttagagg tcgaaattcg cgcagtaagt cgagcttatt tggtgcaaca gcttgctaat
     2701 aaaagtgcgg attatgattt gattttaggt ggttggttag caaataatct tgatcctaac
     2761 agttttttgg aggccttact cagttgtaaa gctgcggaaa cggtgactaa tttatcaaat
     2821 tggtgcaatg aagattttga tttttggtta caaacagccc gtctaagtga tgatccggca
     2881 tcagctaatt tattgtatga atttgcacaa aaattattag aagagcaatt gcctattctc
     2941 cctttaatga atgctaatcg tgtattagtc ataaaagatg gagtgaaaca ggcagatatt
     3001 tcccctttcg gccaggtaaa attatcagag ttacgtttgg gcttggggca taaaaaagga
     3061 aactaattat gctgattgtc ttttttcgaa aaatttttct aaccttgatt acgttgattt
     3121 ttttgtcact tatcagtttt aatattctgt tacgagatcc attaaaccac ttagccgagt
     3181 taggctggtt tgaggcttat tgttattatg taaaatctct gctaaatggt gattttggta
     3241 ttagtttcag taatggagaa ccgctcacag cgcaaatttt acaggtgttc ccggctacaa
     3301 ttacgctttg tttatcggct ctcattgttt ctttaattat tggtttgcct cttggttttt
     3361 ttgcggctac tcagcaaaaa aatatggttg ggcgattatt aaatacacta ggttcattga
     3421 gtttagccgt gccggtattt tggctggcat tagtgctaat gtattacgct tctctcaatc
     3481 agtgggaagt gtcagcaatt ggagaaatcc attcgcttta tccgaaacag atggtaaccg
     3541 gctttttgct attagatatt tggttatctg atgtacctta taaattaaaa atgatgcaga
     3601 gtgctttaca tcacttagct ttgccgacat tagtcttggc tgtgccagct acattagaag
     3661 ttatgcatat tactcaagct agagcaggtt atgtaatgaa acaaaattac attaaaatag
     3721 cgaaaacgcg tggctggaca ccgtttagag tttggagaac gcatattatt tgtaatactt
     3781 tacctgcatt attacctatg attgcccgta cctttatttt aatttttgcc ttcggtatgt
     3841 taattgagaa catatttagc tggggaggaa ttggacgttg gctgataaat gcccttgcta
     3901 ttcaagacta taatgcgatt tcagcgggag tagttgcaat cggggtgttt gtgttattag
     3961 tagatatggt tacaggcttt gtgcgggtga tgttagaccc atcagataaa aaggattggt
     4021 atggcagtta ttaaagaacc cgagcaattt agagaaacca acgcatttaa ggcattttgg
     4081 tttcatttcc gacaagataa acttgcactt ggcagttttt atcttttttt gctattactg
     4141 gctctcattt ttgttggtag tttgcttgct ccttacaatt ataatgaaca atttgttggt
     4201 ttagaattaa tgcctccctc ttgggacgac aaaggtcaaa tcagccattt tttaggcact
     4261 gacgatttag gacgggatat tttaagccgt attatgtatg gtttttacta cacgcttggt
     4321 tccgcactga tgattacctt attaattgcg attatcggcg gtatgatcgg gatttatgcc
     4381 gggctgaaaa aaggacgttc attttggttg attagccatt tgttcgatac ctttttattt
     4441 atgccgattt tattgattgc gatcatcatt gcaaccttaa tggaagcgag tttggtcaat
     4501 gcaatgttgg cgattttctt agcgatgttg ccgcatttta tccataaaat ttatcaagca
     4561 accgaaaaac aactcaaaaa agagtacatc attaccttaa agctagacgg tgcaagctgc
     4621 tggtatttaa ttcgagaagt gattattcct aatcttaccg gcgtggcaat taaagcactt
     4681 tcacatattt ttgtgatcgc catattagat attagtgcgt taagttttat tatgctgggg
     4741 gctaaaagcc cgacccctga atggggagcg atgattcgtg aatccatgga actgatttat
     4801 atagctcctt ggacggtgat attacctggc ttagcaatta tttttagtat ttttaccgtt
     4861 acaatacttg gtacacgctt aagcgtgatt tttgaaaaat accgtaaaga atagtattta
     4921 aataggatac tgacacataa caagcggtca tttttaagta aaattttaca gattaaggaa
     4981 tagtatggca ttacttgata ttcgccattt aagtattgaa attgatatgc caaaaggcag
     5041 agtaaaaatg gtggataaca tcaacctgac tgttgatgag ggcgaaattt gtggcttggt
     5101 gggagaatct ggttcgggaa aaagcctcat agccaaagtg atttgcggta tgattaaaga
     5161 tgaatggatt gtgagtgcag atcgctttcg ctttgacgat attgaattac tgaaactttc
     5221 gccagcggaa cgccgaaaat tggtaggcga tcatatttca atggtattcc aagatccgct
     5281 tactagcctt gacccaagcc agcaaattgg taaacaactg atgcaaacca ttcaatttag
     5341 agggaagtgg tggcaacgat ttgggtggaa aaagaaaaaa gcaatcgagc tactacaccg
     5401 tgttggtatt cgtgatcatc aagatattat gaaaagttac ccaagcgata ttaccgaagg
     5461 cgaagctcag aaagtgatga ttgcaatggc ggtggcaaat cagccccgat tattggtggc
     5521 agatgagccg actaatatta tggaatcaac cacgcagttg caggtttatc gcttgctttc
     5581 cagtatgaat cagaatttgg gaacctcaat cttgctggtg agtaatgaca tgcgtagcat
     5641 tcaaaattgg gttgattctt acaatgtgct ttattgtgga caaaccgttg aaattgcgga
     5701 taaagagagc attcttgaat cgtcttacca cccctatact cgagtgttac tcaatagttc
     5761 gccggacttt tctcagcctt tagcctttaa aagtagctta aatacgctac gaggctcagt
     5821 cccgatgtta caagaaatgc cgattggttg tcgtttaggc cctcgttgcc cttttgcaca
     5881 aaaaaaatgt atcaacaaac cgccattagt gaaagtaaaa cagcacgagt ttgcctgcca
     5941 ttttccgatt aactttagag aacaaaattt gcttaaaaat aaaacggaaa atacgccgtt
     6001 tgtggtggct gaaatagcgg aaaaagaggc gttttaatgt ggttattatt ggctaagtgt
     6061 aacgggtaac attaaagtat ctaatacgaa actcaagggt aaatcgatca ccgcaagcac
     6121 cgcgaatata ccgccttctt gcattacttg gaaatctttt attacgccgg cataaacacg
     6181 gtaatcctgt tcaaccaagc ttagtactgt gccacaagcg gtgaggttaa acatcaaata
     6241 gagtagaaaa acgagtttac aagcggtcaa tttcatcaaa aaatctccaa atacgctttt
     6301 attttacgct atttttatcg ggttggcgta aaattcgttt taaaaacagc tattttcagg
     6361 cagaatgtag ccgaatcgac attaaatata ctaagaggat cttatatgca aacttacaac
     6421 attgcagtgc ttgccggtga cggcattggg ccggaagtga tggcagaagc tatcaaagtg
     6481 cttgaagcga ctcaacaaaa atttggcttt aaactaaatt ttaatcaata taacattggt
     6541 ggagcagcga ttgatgctca aggtaaagcg ttacccgaaa ataccttaaa aggctgtgaa
     6601 gaggcagatg cgattttgtt tggctcggta ggtggcccga aatggacgca tttactacct
     6661 gatgagcaac ctgaacgagg ttcgctatta ccactgcgta aacatttctc gctattctgt
     6721 aatttacgcc ctgctgcatt gtatcaaggg ctggaaaaat tctgcccatt acgtgccgat
     6781 attgctgcta aaggttttga tatggtggtg gtgcgtgaat taaccggcgg gatttatttt
     6841 ggacagccga aaggccgtga tggtgaaggt gcgcaaacga gagcgtttga caccgaagta
     6901 tactacaaat atgaaatcga gcgtattacc cgtgtggcgt ttgaatctgc aatgaaacgt
     6961 aaaaaacacg taacctctgt ggataaagcc aatgtgttac aaagctcgat tttatggcgt
     7021 gaaaccgtca cggaagtagc taaagagttc ccagaagtca cgcttgagca tatgtatatt
     7081 gataatgcga caatgcagct aatcaaagca cctgaatcgt tcgatatttt attatgctca
     7141 aacatcttcg gcgatattat ttccgatgag gctgcaatga ttacaggttc tatgggcatg
     7201 ttgccttcag ccagcctcaa tgagcaaggt ttcggcttat atgaaccggc aggcggttca
     7261 gcaccggata ttgccggtaa aaatattgcg aatccgattg cgcaaatttt atcagccgca
     7321 atgatgttgc gttatagctt taacttaggt gatgctgctg atgcgattga aacagcgatc
     7381 caaaaagccc ttgctgatgg ctaccgtacc gctgatttag ctgatgacag caaaccgctt
     7441 tctacctcag aaatgggcga tattattgcg aaaaatattc tcgcttaagg ctagaagtta
     7501 aatagcacaa gcggtcatta ttcgccaaat ttttgcaaat tttccgctaa taatgaccgc
     7561 ttgtttatta ctcgttagcc gtcaatctct tcgcctgttt cttcccactt aaaattcaat
     7621 gccgcagagt tgaggcaaaa acgcaaccct gttggtggtg ggccgtccgg aaaaacgtgt
     7681 cccatatgcg aatcacaatt cccacaacga atttcgatac gatgacgtcc taaactgtaa
     7741 tcatctaagt aacgtagtga cgattctgaa atcgtttggt aaaaacttgg ccagccacaa
     7801 ccggcatcaa atttagtatc tgagtgaaat aaatcgttat ggcatctggc acaacgataa
     7861 attcctaccc gatgttcatt caaaaatttt ccggtaaacg gacgttccgt accgtggttg
     7921 attaaaatat caacttgttg ttctgtcagg gcgtgaatag gtttaatcat cagcaattct
     7981 cctaagatag tagcgaagtc attgtagctt tttttgacct gaaaatcgtt ataaatttca
     8041 aaatttgaca tagatcacaa aaaatttatc tgttatattg gaagggttct tttggcttgt
     8101 caggaaagtg aaaagatgtg ctataattca agcatcttta gtgtagtaca taaagaacaa
     8161 attttgttta actttaatat aggtagaaaa atctatggca attaaaattg gtattaacgg
     8221 ctttggccgt atcggtcgta tcgttttccg tgcagcacaa caccgtgatg acatcgaagt
     8281 ggtaggtatc aacgacttaa tcgatgttga ttatatggct tatatgctga aatatgattc
     8341 aactcacggt cgttttgacg gtactgttga agttaaagac ggtcaattag tagttaacgg
     8401 taaagcgatc cgcgtaacag ctgagcgtga cccggctaac ttaaaatgga atgagatcgg
     8461 tgttgatatc gcagttgaag caacaggttt attcttagat gatgaaactg ctcgcaaaca
     8521 catcaccgca ggtgcgaaga aagttgtttt aaccggtccg tctaaagatg caacacctat
     8581 gttcgtaaac ggcgtaaact ttgatactta cgcaggtcaa gacatcgtgt ctaacgcttc
     8641 ttgtacaact aactgtttag caccattagc gaaagttatt cacgaaaaat tcggtatcaa
     8701 agaaggttta atgacaactg ttcacgcaac tactgcaacg caaaaaacag tagatggtcc
     8761 atcagcgaaa gactggcgtg gtggtcgtgg cgcgtcacaa aacatcattc cttcatcaac
     8821 aggtgcagcg aaagcagtag gtaaggtatt acctgcatta aacggtaaat taaccggtat
     8881 ggctttccgt gttccaacca ctaacgtttc tgttgttgat ttaactgtaa acttagaaaa
     8941 accagcaact tatgcagaaa tctgtgctga aatcaaacgt gcttcagaaa acgaaatgaa
     9001 aggcgtatta ggctacacag aagatgcagt tgtttcaaca gacttcaatg gtgcaactga
     9061 aacttcagta tttgatgcag cagcaggtat cgcattaact gatactttcg ttaaattagt
     9121 atcttggtac gataacgaag ttggctactc aaacaaagta ttagacttag ttgctcacgt
     9181 atataactac aaaggttaat tgctaagtta tattttaaat atagagccaa attctgaacg
     9241 gaatttggct cttttgctgc gaacaataaa ttgaatatag tcgcgttttc ccataaaatc
     9301 aatttcctac aaacaggaac taagcacagt aaggcagatt ttcttgc
//
LOCUS       DANLUA010000095         9076 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLUA010000095 DANLUA010000000
VERSION     DANLUA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            145..861
                     /locus_tag="PXB44_001639"
     CDS             145..861
                     /locus_tag="PXB44_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5282103.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            complement(931..1809)
                     /locus_tag="PXB44_001640"
     CDS             complement(931..1809)
                     /locus_tag="PXB44_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL5282104.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(1885..3210)
                     /gene="hslU"
                     /locus_tag="PXB44_001641"
     CDS             complement(1885..3210)
                     /gene="hslU"
                     /locus_tag="PXB44_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL5282105.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            complement(3467..3988)
                     /gene="hslV"
                     /locus_tag="PXB44_001642"
     CDS             complement(3467..3988)
                     /gene="hslV"
                     /locus_tag="PXB44_001642"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL5282106.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            complement(4049..4762)
                     /gene="sfsA"
                     /locus_tag="PXB44_001643"
     CDS             complement(4049..4762)
                     /gene="sfsA"
                     /locus_tag="PXB44_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL5282107.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            complement(4778..5647)
                     /locus_tag="PXB44_001644"
     CDS             complement(4778..5647)
                     /locus_tag="PXB44_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5282108.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            complement(5648..6040)
                     /locus_tag="PXB44_001645"
     CDS             complement(5648..6040)
                     /locus_tag="PXB44_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5282109.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            6124..7269
                     /gene="mutY"
                     /locus_tag="PXB44_001646"
     CDS             6124..7269
                     /gene="mutY"
                     /locus_tag="PXB44_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL5282110.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            7273..7542
                     /locus_tag="PXB44_001647"
     CDS             7273..7542
                     /locus_tag="PXB44_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL5282111.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            7559..8650
                     /gene="mltC"
                     /locus_tag="PXB44_001648"
     CDS             7559..8650
                     /gene="mltC"
                     /locus_tag="PXB44_001648"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL5282112.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            8782..8857
                     /locus_tag="PXB44_001649"
     tRNA            8782..8857
                     /locus_tag="PXB44_001649"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8815..8817,aa:Phe,seq:gaa)
     gene            8863..8938
                     /locus_tag="PXB44_001650"
     tRNA            8863..8938
                     /locus_tag="PXB44_001650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8896..8898,aa:Asn,seq:gtt)
BASE COUNT         2636 a         1834 c         1925 g         2681 t
ORIGIN      
        1 agaggaatca agaggattga gaaactacat ctcaatttga tgtgtttgta tgacataaaa
       61 caaactttat gaaaagaaga ctttttttaa gaaagtaact gcgttaaagt ggtaatcctt
      121 ataatgaaaa cacagggact ttttatgaaa aaaacacttt tgaccctttt attcgggtgt
      181 gtggttactg cgcaggcaca agatatcaaa tttgtgatgg agccaagcta tcctccattt
      241 gaaatgaccg aagagaaggg agaaattatc ggttttgatg tggatattgc aaatgctatc
      301 tgtaaagaga tgaatgcaaa ttgtacattc catagccaac cttttgatag cttaatccaa
      361 agcctaaaac aaaaacaatt tgatgctgca atttcaggta tgggtattac ggaaccacgt
      421 aagaaacaag tattatttag cgagccttac tttccaagct ctgctgcctt cattgcgaaa
      481 aaagatacag attttgctaa ggtgaaaacg attggtgtgc aaaatgggac tacttaccaa
      541 cactatttag caaaagagaa aaaagagtac aatgtgaaat cttatgcctc ttatcaaaat
      601 gctattttag atgtgcaaaa tgggcgaatt gatgctattt ttggcgatgt gccggtattg
      661 gctgagatgg caagaaaaca cgaagggcta gattttgtag gggagaaaat caataatcca
      721 aactattttg gtgatggttt aggcattgca actcacttga aaaaccaagt attagttgat
      781 caattcaatg cagcattaaa aacgattaaa gagaatggcg aatatcaaaa aatctatgat
      841 aaatggatgg gcggcaagta acttgtaagc ggtcattttt tgcaaagttt tgcaaagttt
      901 tgcagatttt gaccgcttgt attctttgat ttacccattt aagccagtat aagttttaag
      961 cataagctcc atggtctgct ttaaattagc tagcatttca taacgcttgc gatacattga
     1021 gcgttttttg cttggaattt catctatttt tttactttcc aattcaaggg gtaactgcca
     1081 ataatttccc ggctttccgc ccgattccgc aaaaatagca tcataatcca ctacatagcg
     1141 cttactttgc acccagcggg atttattctg gtatttctgt ggaatgccta gtaaactctg
     1201 atagccaaga acagccgtta aagccttcat cgtttcaacc attaaatagg ccgggcgtaa
     1261 gccgtggcaa cttttggtta attgtttcac cagctctttt gaaccttcaa aattcggacc
     1321 ttgaattacg gctatcaaca aggcttcatc taacttacca aatgttaata aataaactag
     1381 ctgattagtc ggtctgtggc gaagttctaa tgcccaaaaa ccttccatcg gctgatgttc
     1441 attgatgtta agccataact caaaatctgc aataatttca ccaaaattga ccgcttgttc
     1501 ccatagaggt ggtaaatgag ccaatttttg cggtaaaaac agcaggttat ctgtgatttt
     1561 ttgtaagcgt tgttttgcac tgaaacgctt atctaaaaag cggtgagcca cggggtagct
     1621 gtaatttggt cgagcattta agagcggaat cagttgtgga ttttgtgtaa cgaactgctc
     1681 aaattgcttg attttggaat gatgcaacca agagcgaaga cggtaacgaa aacgtttccc
     1741 tgcgtaagac ttctttccct gatttggata aagcgattgt gcagcaggcc atttgtatag
     1801 atttttcatt tttacaacta acaatatgaa aagcggctaa ttcgcaagat ttttcgctaa
     1861 aataaccgct tgtataactt aaaactacaa aataaagcga cttaaatctt cgttttccac
     1921 cacgtcattt aacgcttcgg aaacatagtt ttcatcaatg atgatttttt caccacggcg
     1981 ttcgctggca tcgaacgaaa tgccgtccat caaacgctca agcacggtgt gtaaacgtct
     2041 tgccccgatg ttttcggttt tctcgttcac acggaatgca ctttctgcaa tacggctgat
     2101 accgtcttta gtgaactcaa tctccacgcc ttcagttttc atcaactcac gatattgcaa
     2161 ggttaatgag gcattcggtt cggttaaaat acgctcaaaa tcttctttgg taagcgattt
     2221 taactccaca cgaatcggca agcgaccctg taattctggg agtaaatcag acgggcgagc
     2281 cacttggaat gcccccgagc agataaacag aatatggtcg gttttcacca taccatgctt
     2341 ggtatttacc gttgagcctt caatgattgg aagtaaatca cgctgaacac cttcacgaga
     2401 aacatctcca cccgaatgct cgcctttttt acagattttg tcgatctcat caataaagac
     2461 aataccgtgc tgttccaccg cttcaatcgc ttgctgtttc agctcttcag gatttactaa
     2521 tttcgccgct tcttcatcaa gcatcacctt taacgcatct ttgattttca ttttgcgttt
     2581 tttggttttg cttggcgaca ttccctcaaa cagcgattgt aattgcgagg tcatctcttc
     2641 cattcccggc ggggtcataa tctcaacgct cacttgagct gccacgtcaa tttcaatctc
     2701 tttatcgtct aattgccctt cacgcaattt tttgcggaaa gtttggcggg tagaattatt
     2761 gtcggattcc tgcacatttc cccactgatc tttcgccggt ggcaataata cgtctaaaat
     2821 gcgatcttcc gctgcatctt gtgcacgcat tttatttttt tcgaccgctt gttggcggac
     2881 taatttcatc gacacatcag ctagatcacg gataatcgaa tccacttctt tacccacata
     2941 ccccacttca gtgaatttgg ttgcctcaac tttgataaaa ggggcattgg caagcttcgc
     3001 caaacggcgg gcgatttcgg ttttacccac accggtcgga ccgatcatta aaatattttt
     3061 gggggtcact tcttggcgga gatcttccgg caactgcatt cttctccagc ggttacgcaa
     3121 agcaattgct accgcacgtt ttgcatcttt ttgtccgata atgtgtgcgt ctaattctga
     3181 gacgatttca cggggtgtca ttgacataat gttattcctt aaattaatta ccctctcccc
     3241 ttgtgggaga gggacagatt tttctttgtt cagaagaaaa atcagggaga gggtaacaag
     3301 cggtcttttt tcttaaacgt tttgcattaa cccctctctc tgaaaaatat ttctgaacga
     3361 aatatttttc ggtctcaata ctcgctacgc tcgctcctgt ggagccgttg gcaaagccaa
     3421 cgttcaaatg taaacatttg tcccacaagg ggagagagtt atttaactaa acttcttcaa
     3481 tcacgtgatt gtagttactg taaatatcaa tatcgccggc gatttttaac gcttccgcaa
     3541 cgatttcttt tgcagataaa ttattttcgg tgcgtaataa cgcaagagca gcggctttcg
     3601 catagttacc gcctgaacca atcgccagca catcttgctc cggctcaatc acatcgcccg
     3661 aacctgaaac caataaaaac tcgctgtcat tggcgacaat catcatcgct tctaaacggc
     3721 gtaaagcacg ctcggtacgc cactcttttg ccagttccac tgccgatttc actaaatgcc
     3781 cttggtgtaa ctccaatttt ttctcgaata aatcacgcaa aataaaggca tcagcggtcg
     3841 agccggcaaa gcctgtcacc actttgtctt tatacaaacg gcgaactttc cgcaccgtgc
     3901 ctttctcgat acaatttcca agcgttgcct gaccatcacc accgatagcc acttttccgt
     3961 ctttacgcac gcatacaatc gttgtcatac taaatccttc tttttaaaaa ctttggctta
     4021 tatggggata tgctgtgtgt tttcaagctt aaaactcaca tttctttgtt aaggttgccg
     4081 caataggttg cccgttttcc aatgaaattg ccattttata ggagagaacc tgcacttttt
     4141 gtgcaatggc ttgagcgaat aattcggcat atttggggtc tatctgcttt gccacttcaa
     4201 accgttcaat acctgtgtgc aaaatagcga aaaaaatgac cgcttgtaag ccctgttcag
     4261 cgattttcgt cagctcccgc aaatgttttt gcccccgctc ggttttggca tcggggaaca
     4321 ttcccacacc gttttcggtt aaaagggtgg tagatttcac ttccacaaaa caatccggca
     4381 gaaggtccga tttgagcaaa aaatcaatcc gactgttttc actgccatat ttttgttcag
     4441 gcagaatgtg ctgataatcc gcaagctcgc taatccattg attttccaag gcttcttgta
     4501 caagctgatt ggctctcaag gtattgacac aaataaaatc ccccgcttgg gtttgggtta
     4561 gttcccaagt gtgagcatat ttacgtttgg ggttatgaga ggtggaaaac caaactgtat
     4621 caccggggtt ggcacagcct gtcatcgcac cggtatttgg gcaatggagg gtaatttgtt
     4681 cgccatttgg caactgaata tcggcaagaa aacgtttata acggcgaagt aaaatgccgt
     4741 gggaaagagg gggaaatttc atttgcaaaa aatcctgtta aattaaccgc ttgtatcttt
     4801 tcatcatcgg tagcaaaacg gagagtaagg cggtgcaaat aacaataata atcccaaccc
     4861 aagtcactaa actgagctgt tctgataaaa agaccaatgc cattagcagt ccgaataccg
     4921 gttcaagtgc gagtagaata cctgcaagaa taggcgaaga ttggctgatg cctttgttcc
     4981 ataacaatat tgccaagctg gtacaaataa tgcctaaata tgctacgctc gcccatgctg
     5041 agagactggt tggcagttgc caattttctg caaagagtaa ggtaataggt aaggcggtaa
     5101 ttgccccttg caccagcatg atcttggtta aatcgatcac ttttactgat tgcagaatct
     5161 ctttgctgag gtacattccc acaataaaac ttaaactgcc taaggttaca agcaataatc
     5221 ctaatacatt gattgaaccg ctatcatcgc tacccgatgc cataattaaa atccccaagc
     5281 aggcaaataa acttaggata aaatcgagcc tggttgcctg ttttttgaaa caaaaatagc
     5341 cgactaacac agttaaaagc gggtttagcc cgagtagtgc cacggcactt gctgcggaag
     5401 ttaatttcaa gccggaaaat tgtaagatca cgtacagagg aaaagtgaga aaactaatca
     5461 gccagacttt aatgtgctga tttttctgga tttttttata cgcaggaata aaaaacggca
     5521 gagcgataaa cgccgctaac gtcagccgaa tttgcataat ttggacaggg tcaaaggctg
     5581 aaatcgccac tttacctaca atgaatgaac tcgcccaaat cagggttgca atcagtaaat
     5641 ataacattta gacctcgtaa aactctaacg gcagatcgtc cggatctcgg aaaaacgtga
     5701 agcgtttgcc ggtgatctca tcaattcgga tcccttcatg cggtaattga tgttgttcta
     5761 aaaatgcgat ggcatattca atattttcca ctcgaaatgc caaatggcga agcccgcagg
     5821 tttcgggcga ggtgaaacga ggcggcgttt caggaaaact gaataattca atttgtgagc
     5881 catctttaaa gcgtaaatct aatttatagc tggcacgttg ctctcggtag gtttctgcga
     5941 tgatctctgc ccctaaaatg cgggtgtaga aatgtttgga tttttcgtaa tcggaaacaa
     6001 ttaatgcaat gtggtggaaa cctaaaatgg gcgatttcat cttttttcct taatgtgtaa
     6061 aatttttggt aaaactgacc gcttgcagta tgataattta tttttcggat ttcaagattt
     6121 ttaatgaaca gcaattcccc atttccttta gcgaaagcaa aagcttctga tccgtttgcc
     6181 aaagctgttt tagcttggtt tgagcaatat gggcggaaac atttaccttg gcagcaaaat
     6241 aaaacgctgt atcaggtttg gttgtcggag gtaatgttgc aacaaactca agtagcgacc
     6301 gttattcctt attttgcacg ttttatggaa cgatttccca ccgtaattga cttagctaat
     6361 gcctcgattg atgaagtatt gcatttgtgg acagggctgg gttattacgc tcgggcaaga
     6421 aatttgcata aagcggcaat tcaaattcga gatgagtttg gcggtgaatt tcctacccga
     6481 tttgatgatg tgttggcatt aaccggagtg ggcagaagta ctgcaggggc gattttgtct
     6541 tctgtgttag atgccccaca cccgatttta gatggcaatg tcaaacgggt gttaagccgc
     6601 tattttgcag tagaaggctg ggcgggtgaa aaagcggtgg aaaatcgcct ttggcaatta
     6661 agcgaaagtg tgacgcctga tacccaagtg gcagacttta accaagcgat gatggattta
     6721 ggggcgatga tttgcacccg tacaaagcct aagtgcttgc tctgcccgct acaagaaaat
     6781 tgtaaaacga atgcaatgca agcgtgggat agctttcctg ccaagaaacc gaaaaaaacc
     6841 ttgccggagc gtcaaaccta ttttttagtg ctgaaaagcg gctcgaaagt gctgttacaa
     6901 aaacgggaag caaaaggcat ttgggggggc ttatttgtgt ttccgcaatt tgagagctta
     6961 gaggctctca agcggtctga ttttatgcaa aatttgcaaa tttcacagca attaactgca
     7021 tttaggcata cttttagcca ttttcatttg gatattaccc cggttttagt agaaaccgat
     7081 ttgaaaaaaa caagtgaaaa ttcaccgctt gttgtcgaag aaaaagcagg aaattatctt
     7141 tctaaggtat cttcaaatgc ggattattgg tatgatttgc accaacataa cgaagtgggt
     7201 ttagcaacgc cggttaaacg tattttagat gagctggcat tcagtgtgaa aaatgagatt
     7261 aaggattaaa ttatggctcg taccgtattt tgcgaatatt taaaacaaga ggcagaaggt
     7321 ttagattttc aactttatcc gggggagctg ggtaagcgta tttttgactc aatcagcaag
     7381 caagcgtggt ctgagtggat taaaaaacag acaatgatcg tcaatgagaa aaaactcagt
     7441 atgatgaacc ccgaacaccg taaactgctc gaacaagaga tggtcaattt tttatttgaa
     7501 ggtaaagaga tcatcatcga aggctataag ccggtcgaat aataaaggtt aaatcacaat
     7561 gaaaaaatat gctaaatatt tgccgatgtt ggcaattatt ccgtttctta ttgcttgtgg
     7621 gagcaataaa tcgactaaga agccaaaatc tacccgagta gattattcta aagataccaa
     7681 tggtttggat attttggcag ggcagttttc tcacaatatt gatgatattt ggggaagtaa
     7741 cgagctgtta gttgccagta agaaagatta tgtgaaatat acggataagt tttacacccg
     7801 tagccatatt tcgtttgaag aaggggtgat tacgattgag actttaggga atcagaatca
     7861 tctacgcaat tcgattattc atactttatt aatgggcaga gacccgaaag ggattgattt
     7921 atttgcctca ggcgatgcac cgattagctc taaccctttc ctaaacggac aggttgaaga
     7981 tcaattcggg cgaaatatta ccaatgttgc catcgccaat gattttgcga cttatttgtt
     8041 gcaaaataag gtgcaaacac gccgtttgaa aaatggtcaa actgtgagct ttgtaacgat
     8101 taaaatggtg gcaggacaca ttgaagtacg tgccagacaa tatttaccgc tggtgcgtaa
     8161 aatggcgaag cagtatggta ttgagccaag tttgatttta gggattatgg aagtcgaatc
     8221 agcattcaac ccttatgcca tcagctatgc caatgcgatt ggcttaatgc aggttgtacc
     8281 tcgtaccgcc gggcgtgata tttttgcccg taaaggtatg ggagggcagc caagtcgtga
     8341 atatctttat aatccatcaa ataacattga tgcaggtacg ctttatttga ctattttgcg
     8401 tgatgaatac ttagatggaa ttgaagatcc tgtggctaaa cgctatgcaa tgatttctgc
     8461 atacaatagc ggagcaggtg cggtattacg agtgtttgat tcagaccgat actctgcgat
     8521 tgatagaatt aacgatcttt cgtctgatgc ggtttatcga attttaacca ctgctcatcc
     8581 atcttctcaa gctagaaatt atttaagaaa agtaaccacc gccagagaga aatatcgcaa
     8641 tattcgttaa tgtgtgaatt atgctgtttt ttagccgaaa agtataaaaa aagcacattt
     8701 gagcagcggt ttttttaaat ttgcaaaaaa acgtttgacg ttgcaagctg aaatcagcat
     8761 aatgcaccac gcaacgacgt tgcctcgata gctcagtcgg tagagcaggg gattgaaaat
     8821 ccccgtgtcg gtggttcgat tccgcctcga ggcaccatca attcctcctt agttcagtcg
     8881 gtagaacggt ggactgttaa tccatatgtc gcaggttcga gtcccgcagg aggagccata
     8941 ttctgaagcc ctgatgagta atcatcaggg ttttcttttt gagctttccg gctaactact
     9001 aagaaattgc tcaattttta gtattatgcg ttcaaaaacg ctaaattttt gagaattgta
     9061 tttgacgaag cccgaa
//
LOCUS       DANLUA010000096         8917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLUA010000096 DANLUA010000000
VERSION     DANLUA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..186
                     /gene="zapB"
                     /locus_tag="PXB44_001651"
     CDS             <1..186
                     /gene="zapB"
                     /locus_tag="PXB44_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL5282113.1"
                     /translation="KIKQAVETIQLLQLEVEELKGKNDAAKQENDTLRSEYEQLKVEQ
                     QNFQDRLRSLLGQIDNV"
     gene            242..823
                     /locus_tag="PXB44_001652"
     CDS             242..823
                     /locus_tag="PXB44_001652"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL5282114.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(914..2032)
                     /locus_tag="PXB44_001653"
     CDS             complement(914..2032)
                     /locus_tag="PXB44_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL5282115.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(2122..3570)
                     /locus_tag="PXB44_001654"
     CDS             complement(2122..3570)
                     /locus_tag="PXB44_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5282116.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(3717..5414)
                     /locus_tag="PXB44_001655"
     CDS             complement(3717..5414)
                     /locus_tag="PXB44_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL5282117.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
     gene            complement(5558..6109)
                     /locus_tag="PXB44_001656"
     CDS             complement(5558..6109)
                     /locus_tag="PXB44_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL5282118.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEF
                     WSNNSALIISLLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
     gene            6208..7017
                     /locus_tag="PXB44_001657"
     CDS             6208..7017
                     /locus_tag="PXB44_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5282119.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
     gene            complement(7034..7726)
                     /gene="tsaB"
                     /locus_tag="PXB44_001658"
     CDS             complement(7034..7726)
                     /gene="tsaB"
                     /locus_tag="PXB44_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL5282120.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7813..8895)
                     /gene="aroG"
                     /locus_tag="PXB44_001659"
     CDS             complement(7813..8895)
                     /gene="aroG"
                     /locus_tag="PXB44_001659"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL5282121.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
BASE COUNT         2469 a         1982 c         1882 g         2584 t
ORIGIN      
        1 aaaattaaac aagctgttga aactattcaa ttacttcaat tagaagttga agaattaaaa
       61 ggtaaaaacg atgcagcgaa acaagaaaac gacaccttgc gcagcgaata tgaacaatta
      121 aaagtggaac aacaaaactt ccaagatcgt ttacgttcac ttttaggtca aatcgacaac
      181 gtttaattct attttcagca caaacggcta aatttttagc cgttttttat ttgttacagc
      241 tatggcaaaa ctctattttt actactcctc aatgaatgca ggcaaatcta ctactctgct
      301 tcaatcttct tataattacc aagaacgtgg aatgaacact ttagtttata ccgccgctat
      361 tgatgaccgt ttcggtgtgg ggaaagtcaa ctctcgtatc ggtatttcac aagaagccaa
      421 tttatttacc gctgatacca atctatttgc ggaaattaag gcattacact caaacaaagc
      481 gttacactgt atcttgattg atgaggctca gtttttaact aaaacacagg tttatcaatt
      541 aagtgatgtg gtcgataaac tcaaaattcc ggtgctttgc tacgggctac gcaccgattt
      601 ccaagctgaa ttatttgaag gtagcaaata tttgttggct tgggcagatg agctggaaga
      661 actcaaaacc atctgtgatt gtggcaaaaa agcccatttt gtcgttcgat taaatgaaaa
      721 aggcgaagcc attaaagacg gtgaacaaat acaaatcggc ggcaatgata gatatctctc
      781 tgtttgccgt tatcattaca aacaaaagtt aggtaagcta taagctacaa gaagcagcaa
      841 gcggttaaat tttcgggaat ttttgcaaat tttcagcgaa atttaaccgc ttgtttgtgt
      901 tatttacctc tctttacttt cctgcaaacc gaatttgctc actcatttgt ctcatcacac
      961 tcgaagtctc ggcaaaaaac tgttggtaag aggggcaaag gtagttgtgg ttttcgctgt
     1021 ttgcttgggg gacgatgcgg tgtttggggc agccgccgtg gcaaagtatg cggaatttgc
     1081 agtttaggca ggcttgcgag agttttgcct gtttattctg cccgaattgg cgttgggcgg
     1141 ctgatgagac gatctctgcc agctgctgtt ggttgaggtt gccgatgtgg taatcgggat
     1201 agacgaaatg gtcgcaactg tacacatcgc cacaggcttc caccaccagc gagctgccac
     1261 aggtcgggcg gaatacacag ttgttggagg gatagccaag ccattgcccg agcaggttat
     1321 caaactcgat cacaaaaatc tgcccacggt caggcagcca ttcgtgaaaa acatccacta
     1381 aaaattgccc gtagccttgg ggcggaacgg aaaagggttg taattccccc ttgtggcttt
     1441 ccaccaccgg aatgaactgc atataacggc taccgagggc tttgagggct tgataggttt
     1501 ctctgccccg ttgccagttg cggtcgttca ccaccgtgag ggtgttaaat tccacgccaa
     1561 cctctttcaa taaatcgacc gcacttttca cccgttcaaa ggtcggttta ccgttggtgg
     1621 aaatgcggta gcggtcgtga atgtcgctca gtccgtcaat ggataagccc accagaaaat
     1681 gattctcttt gaaaaagctc gcccactggc ggtttagggc aatgccgttg gtttggattg
     1741 cgttggtgat ctgcttgccg tttgcaaatt tttgttgata tttgaccgct tgtttgtaga
     1801 aatctaagcc cagcagggtc ggctcgcccc cctgccacac aaaatccacc cgttggcttt
     1861 ggttggcttc aatgtaatga cgaatgtagt tttgcagcgt ttcatcgttc ataaactgcg
     1921 atttttctgg cggaatggtc tgttccttgc tcagataaaa acagtactcg cactgaatat
     1981 tacagtgaaa actgctgggt tttgccatta ggtggaagta ggtggtggtc attgtatctc
     2041 taaattgtag gggcggactt tatgttcgcc cattgtttac attaattttc gggcgggtat
     2101 aaaacccgcc tctacaaaca attatatctc atcaataatc cgatacaccc aattttccag
     2161 tgggtcgttg aggcgtttca tttcctgtcg catttcttcc aacatctctt ttttcacggc
     2221 ttggtaggct tcgtcataaa agaggttgtg catctcttcg gggtcttttc tgacatcgta
     2281 gagttcgcag cggtcggtgg cgttgaagac caatttgtaa tccttacgcc gccacattcg
     2341 ctgttcaaaa tagacaaaat gccctgccag ttggcacaac aagcctttgc ggtcgttgtt
     2401 ttggctttgt ctggttatcg gcagaatact ctcgccttgg aaagcggtcg ggatcggttg
     2461 atttgccaca tcatacacgg tggaagtgag atcgtgcaaa taaacgaggt tgtcatcgac
     2521 ttcgttcacc cttgtagagt gcggatcttt aataatcagc ggaatgttgt aggtggtttc
     2581 aaacataaac tcgccttttt cgatcatctt atgcgccccc atcgcatcgc cgtggtcggc
     2641 ggtagcaacg aggaaggtat tttcatagag attgttctct tccaagaaac ggaacagctc
     2701 gccaatggca tcgtcaatca gcgtgatgta gccccagaat ttagaaatca cctctttcca
     2761 acgatcttcg ctcgcctccc acattcccca catttgtgaa atggtgcgga agtggttcgg
     2821 tttgccctca agcggtttga agaaactctc gtccaatacc acttggcttg ggtcatacat
     2881 tgaataatac ggctccggca ctacacacgg agtgtgcggc ccccagaagt tgatccacgc
     2941 aaagaacggt tttttctcat caagggaggc ttgaatgtat tttttcgcct cgtcaatgat
     3001 aaaccactct attgtctgct cgcgactgcc ggaaagcaaa ccacagagtt cctgcacacg
     3061 caggtgtgga ttgtcgccaa aataggctcg actgacttcg ggtgcgtcat agcccttttc
     3121 cgccagccat tcacgataac ggttggagtg tgttggcggc tgattaaaca cgaggttttt
     3181 atacacttgg ctgcccgggt agccatagcc gtcaaagtta tgaccggcga tgccataatc
     3241 ttcgggaacg gattttgtac caacgtgcca tttgccgatc acaaaggagt tatagccctc
     3301 cacgccggca atattagggt tttgttgtgg aatttcgcct gtgccgcctt tttcgccgtt
     3361 tttgataatc ccgtggctgg acggcattaa gccggtaaac agcgaggtgc gagccggccc
     3421 gcagaccgaa gccggcgtaa aggcgttgtt aaaccgaatg ccgtctttcg ccaagcgatc
     3481 caaattcggg gtttttacca cctgatgccc gtaagtgccg agcatatctt tacgcacttg
     3541 atctaacaaa atatagatga tgttattcat aatgacctct ttaaggcttg ttaataaccg
     3601 ataaattatc aagtttcgga gatctgttcc cctctttagc aaagagggga tttttgatct
     3661 cgagcctttt ccgttcttgg tatttttata ctaatgagga gaaggctcac attcatttaa
     3721 ttcaccgcct ttttgccttt acattttgca aaaaacacga cacaaaccac cgcttgtagc
     3781 acagcataca ccgccagcac cattgccgga ttgccgtctg gtgaggctaa accgagcgga
     3841 gagaggaaag cgtatagcgt gaataagccc aacacgatag aagccactgc cagtggggca
     3901 tattcccagc tggttaagtc cacattggcg tggttgattt tgttgtcttc gtatggagtc
     3961 gcacgtttta ggaaaattcc taaaatcagc attaacgcca catcgaacac gaacaagcca
     4021 gcttgcacat acacgaagtt gatttttaca tcaaacaccc aaacgatgca gaagtagaga
     4081 ataacgtgtg cgagtaaggc aatgcgtgcg gctttgcctg agacggtttt gttgaataag
     4141 ccgatgacaa acagtgccac aatcggaatg ttcacaaaac cggcgaaacg tttggtcagc
     4201 aagaaaatac cgtctgtgcc gaacatcagt aacggcccaa agatcatcgt aatcaccgcc
     4261 attaccgcag agactttttt cgcataactt atcagttccg catctgtgaa ctgttttttg
     4321 ctgatagacg gcagtaagtc tttacagtaa atggttgccg ctgagtttaa gaacgagttg
     4381 aaggtactca aaatcgcccc gaacagtgcg gcaataaaga agccttgtag cacggttggg
     4441 aaagttttat tcactaattc agggtaagac acatcaatcg gtttcaagcc ctcacctaaa
     4501 atatggaaac tgagtaagcc cggtaagttt aagataagcg gtagcaataa taagaacagt
     4561 gccgcaatta agatcccttt ttgaccggct tttaagtctc tcgcccccaa ggcacgttgc
     4621 acaatggctt ggttggtcgt ccagtagaag aaatgcacca ccataatacc ggtgaaaatg
     4681 gtcggccaag gcactgcatc ggtggcacta cccaccgcat tccatttttc aacgtgagta
     4741 gtggtgatga ttttcacccc ttctgccata ctgccattac ctaaatagat aagtgcaaac
     4801 accggcacta ataatgcccc aatgactaac aagaccgcat tccaagtgtc tgacaccgcc
     4861 accgctttta agccaccgaa aatggcataa atcgccccaa caataccaat cgcaatcacc
     4921 gtataggtaa tcgctgcccc atagcttaag ccgaaggcag tttcaaggtt gaagatttta
     4981 ttgaaggcaa tcgcaccggt gtaaagtgca gtcggaatga cgatgaaaag atagaacacc
     5041 aaaaacaagc ccgacataat caaacgggtt tggcggtcga aacggttttc aaagaactcc
     5101 ggaatggtgg tataaccgtt gcggatatat ttcggcaaca gataaagtgc caagaagcaa
     5161 agcggaatta ccgccggcac agtccacgca ataattgaga aattaccggc ataagagttg
     5221 gcattcacgc cgataagttg ctcggtagaa agggaggtta agaccatcga acagccgata
     5281 accgtagcac tcaagccacg ccctgccaag aaataacctt tagatgtcga aagatcgtcg
     5341 tttctggttt ttagccaaga gatatacccg actaatccag ttacaaccag aaaggtaatt
     5401 attgttaata acatattctt cacctcaata tgactatata aatgatatat cccattagag
     5461 acggcaatag cataaatctt atttttaacg atagaatatg atgtttgtca taatagatat
     5521 gtgatggtga tcacaactat gaataattta tgacaattta actgattttc tttaattctc
     5581 gccttaatcg aatggctgtt tcggtttcac aacctgcaaa ttcggcgatt tcacggttag
     5641 tccagtgata attgggataa cgttggtgta aataatgaat actgttcatt acccgctctt
     5701 gcgttttttg ataggaaacc attgataaac gatgctctgc gtcttgtaat tctgtcgaga
     5761 gctgttttaa cagagagata attaaggcgg aattattcga ccaaaactca atgaaattta
     5821 agggggtaat acgtatcaat tctgccggct gtaacacccg agccgagcaa tggtagcgat
     5881 tatcgccaaa aaaggttcta aacccaaaat aatcgttggc tttatatacc ctcaccaagc
     5941 tctctttacc attttcaagc atatggtaca gccctattaa tccgcttttt aaaaaataaa
     6001 attctctggc gtgataaccc tgagaataaa tgacgactcc tttttctaga tcagacatta
     6061 cacttaattg gcaagacatc aattgttcgg gggttggcat attgttcatt gattatcctt
     6121 attattttat tttttccata tatgattgcg atcataatcc tttatttaga aaattttgca
     6181 tacttatcac actttaggag atggattatg acaatgaccg tgataattat tttgatcatt
     6241 tgtggcatta tagtcaatat cacttctgca ctatttggga ttggcggggg tgttttaatg
     6301 atgccgattt tacattcact gtttcctgaa ctgcctctcc aagtgatttc cgcctgctct
     6361 cttaccattg tgattggttc agccactatc aatttatgct tattccacca tcaacagata
     6421 aaaatcaatc gaaaaaatgt gttgatttgg tcacttgcga tgatgttagg cgtacaaatt
     6481 gggtttgaac tcagttttct tttctctgat aacagcatta tcgctatctt tattctaacc
     6541 ctgctgacac tttcatttaa aaccctattt cacaaaccaa gagttaaagc aatagtacca
     6601 cctaaaccaa aagaagcagt atctaatgcc acaatgtgca gtttaggcgg tttaattgca
     6661 ggtattaccg gcattggcgg cggttcgatt atggctccga ttgtcacgca aatacgctcg
     6721 gtttcaccga aacaggttgc agtttatact aactatatga tggtgattgg cggaatcggc
     6781 aatttatatg gctacttatc ccgccctttt gtgttgaatt caaccttagc aacttggcaa
     6841 atcggctatg tgaattttgc aattgtcggc accgtagtga taagttctct ctttactgtc
     6901 tatttttcaa tgaaattacg caatatcttg agtcccgaat taactactaa attacttggt
     6961 gtaatcctat tcactattgc ctgttatatg ggctttttgc aatatttatt cacttaacca
     7021 taagttcaat agattattgc ttatgaggta gtttttccca agtaacttca ttacgcaaat
     7081 acaccggttc tatttcaaaa gcagattgga tattgccttt ttgaatctca gtttcagcaa
     7141 tcgaaagcat atatcggctg gaaggcaagg taatatcact gataacaagc ggtagatttt
     7201 gctcagaaaa ttgcgaatat gccgcccaac ctgtgccaac caccacagtc tcttcactca
     7261 tttggaattg acttattgcg acttctggcg aacagacttg ttcagccacc acttcattcc
     7321 attcttcacc ctttcgttca aattgagcaa agtaaacttc attcattcgg gcatcaatta
     7381 aggcaattac ctttgttgca cctaattttt ggtatgcttc ttctgccatt gcttttaagt
     7441 tagaaacagc taccaccggc aaattcgctc ccattgccaa cccttgtgcc acacttacac
     7501 cgaccctcac accggtaaaa ctgcccggcc cacgtccgaa tactaaataa tttacttctt
     7561 tgagttcgat attggcttga ttaagtaatt catctaccat cggcaaaata cgttgagtat
     7621 gggaacgagg gctgatttca tctaagaacg tgatatttcc gttatactgt aacgctacag
     7681 agcaagcctc tgttgcagta tctaaagcaa gaatgctggg tttcatcata tgtttagttt
     7741 gaataaatta aatttgcttt aaggttaaac aaataaaggg cgaattatat tcgccctcgg
     7801 ttcaagaaaa gattattttg caaaatttct gcgcttttca accgcttcag caagtgcgaa
     7861 taacgctttt tcactatctt cccagccaat gcagccgtct gtaatacttt gtccgtaaac
     7921 taacgcctct aaacctttgc cttcaattaa gtcttggcga ccttctacca agtggctttc
     7981 aatcatcaca cctgaaataa agtctgatcc acctgcaatt tggttgcaca catcagcaca
     8041 aacgtccatt tggcgtttga actgcttctg gctgttagcg tggctgaaat cgatcatcac
     8101 gtgtggacgt ccgccggatt tctcaatcgc agcacaagcc gctgcaacgg agtcagcatc
     8161 gtagttggtt gcgctatcgc caccacgcaa aataatgtga cagtcaggat ttcctgctgt
     8221 tgaaacaatc gcagaatgcc cgaatttagt gaccgataag aaatggtgtg gcgattttgc
     8281 ggaagaaatt gcatctaatg caattttcac actaccattc gtgccgtttt taaaacctac
     8341 cgcacaggat aaaccagagg ctaattcacg gtggacttga gattcagtcg ttctcgcccc
     8401 aattgctccc cagctcataa agtcagccac atattgtggt gtaatcatat ccaaaaactc
     8461 gccggcagtc ggcacgctta aatcattaat atcagataat actttacggg caatgcgtaa
     8521 accatcgttt aaggcaaagg tttcgttcaa ataagggtcg ttaattaagc ctttccaacc
     8581 aacggttgta cggggttttt caaaatagac acgcatcacc acttctaagt tttggttaat
     8641 tttcggatca gcacgcattg ctttaatttt ttgtgcatat tctaatgccg ctttcgggtc
     8701 gtgaatggag caaggtccaa tcaccactaa taagcgatca tccgcaccgt gtaaaatttt
     8761 gtggattgat tgacgggttt tagcaacggt ttccgctgca acatcacttg ccggataggc
     8821 ttccagtaat gcaataggtg gtagtaattc ttcaatactg gtaattctga catcgtcatt
     8881 tttgatataa gacattgtgt tcactctcta aataaaa
//
LOCUS       DANLUA010000097         8849 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLUA010000097 DANLUA010000000
VERSION     DANLUA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            15..1001
                     /gene="cas1c"
                     /locus_tag="PXB44_001660"
     CDS             15..1001
                     /gene="cas1c"
                     /locus_tag="PXB44_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL5282122.1"
                     /translation="MTTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHIFCFGNVLVS
                     PFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNPVPVARNIIA
                     AKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGIEGDAAARYF
                     GVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGVGLDPQIGFL
                     HADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAVTLKPEARKL
                     LFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPFLMR"
     gene            1068..1361
                     /gene="cas2"
                     /locus_tag="PXB44_001661"
     CDS             1068..1361
                     /gene="cas2"
                     /locus_tag="PXB44_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL5282123.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1553..2522
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1553..1572
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2772..4247
                     /gene="rng"
                     /locus_tag="PXB44_001662"
     CDS             2772..4247
                     /gene="rng"
                     /locus_tag="PXB44_001662"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL5282124.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4401..5903
                     /gene="lysS"
                     /locus_tag="PXB44_001663"
     CDS             4401..5903
                     /gene="lysS"
                     /locus_tag="PXB44_001663"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL5282125.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
     gene            complement(5981..7054)
                     /locus_tag="PXB44_001664"
     CDS             complement(5981..7054)
                     /locus_tag="PXB44_001664"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL5282126.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
     gene            7322..8005
                     /locus_tag="PXB44_001665"
     CDS             7322..8005
                     /locus_tag="PXB44_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5282127.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLVVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
     gene            complement(8042..>8849)
                     /gene="purU"
                     /locus_tag="PXB44_001666"
     CDS             complement(8042..>8849)
                     /gene="purU"
                     /locus_tag="PXB44_001666"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL5282128.1"
                     /translation="CPDDTGLVAKITNICYKHQLNIIKNSEFVENETKRFFMRTELNG
                     IFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGDLLMKNYYGGLDVEI
                     AAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDEYSPDYIVLAKYMRV
                     LNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGATAHFVNDELDEGPII
                     MQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYKNKTIIL"
BASE COUNT         2647 a         1607 c         1958 g         2637 t
ORIGIN      
        1 aaaacaccct ctacattacc acccaaggta gctatttgca caaagagcga gaaaccttgg
       61 tggtggagca agatcggaag aaagtggcac agttgccgat tcattccatt gggcatattt
      121 tttgttttgg caatgtgtta gtgtcgccgt ttttaatggg attttgtggc gaaaataacg
      181 taaatttagc gttttttacc gaaaatgggc gttatcttgg gcggttgcag gggcggcaaa
      241 gtggtaatgt attgttacgc cgagcccagt ataaaaagtc ggagaccaac cctgttcctg
      301 tggctcgcaa catcattgca gccaaaattc aagcatctaa gcgggtgtta caacggcaaa
      361 ttcgtaatca cggcgaaaat gctgaccttc aatcgacaat taccgcatta aattacagcc
      421 tgcaacagtt gaaaacagca gacaatttgg atttgattcg tggcattgag ggcgatgctg
      481 cggctcgtta ttttggggtg ttttctcatc ttatcaaaga aaatagcggt ttccactttg
      541 acggacgtaa tcgccgccca cctcgtgacg gtgtgaatgc gttgctttca tttctttata
      601 gcattgtagg gaaagatatt agcggggcat tgcaaggcgt ggggcttgat ccccagattg
      661 gctttttgca tgcagatcgt tcggggcgag atagcttagc tcaagatatt ttggaggaat
      721 ttcgagcgtg gtgggtggac agaatggtgc tgtcgctgat caatcgaggg caggttaagc
      781 ctgatgattt tgtgacggaa gccagtggtg cggtaacgct caagcctgaa gccagaaaat
      841 tactatttca aaccttgcaa gcgaaaaaac aagaaaaaat catccaccca tttttagagg
      901 aagaagtgga aatcggcttg ttaccttata tccaagcgat gttattggct cgtcatttgc
      961 gtggcgattt ggcagaatat ccgccgtttt taatgcgtta aaatctgcaa aattttgttg
     1021 aaatttgacc gcttgtttat gttttattca ttgaggaatt agcaactatg ctgatgttga
     1081 ttacctacga catttcattt gatgaccccg aagggcaggc tcgcctacgc cgtattgcta
     1141 agcattgttt ggattatggt gtgcgggcgc aatattctgt gtttgagtgt gatgttactc
     1201 ctgctcagtg ggtaacattg aaaaataaat tattggcaac ttacaaccca gactgtgata
     1261 gtttgcgctt ttaccattta ggcagtaaat ggaggaataa agttgagcac catggcgcaa
     1321 aaccttcagt ggatattttt aaagatacgc tgattttata aatcgctagc ctgtagttct
     1381 cataaaaatg tagcaaggtt agcgaatcta tattgttctt taataatcaa ggaattagaa
     1441 aaaataaggc acaatatatt ggcgatatgc tatactttat ccactctcta caaaacgtta
     1501 gcgaataaag ggctaaaaag cctgatattt caatacgtta tcttataggg accagccacc
     1561 tacgcgtggc tgcgaattga aaccctataa cgagcaatgg cattttgatc tacggtaaac
     1621 cagccaccta cgcgtggctg cgaattgaaa cctttttcaa gaatcaagat taagagatcc
     1681 aaacaaccag ccacctacgc gtggctgcga attgaaacca ccgctgctac ttcggttatc
     1741 ggagtagttt gcacccagcc acctacgcgt ggctgcgaat tgaaacgtgg cacagtaaca
     1801 cgagcagatg ctgataaacg cccagccacc tacgcgtggc tgcgaattga aaccagcagc
     1861 cgttgttacc gtggtgcaat gcttgcgcca gccacctacg cgtggctgcg aattgaaact
     1921 acacttattg ccatagggta gtagtaatct tttacccagc cacctacgcg tggctgcgaa
     1981 ttgaaactgc gatttttgga tttttcgggc gataaatgat accagccacc tacgcgtggc
     2041 tgtgaattga aactcgtggc tggttataca aagaaaagca ttgttgggca gccacctacg
     2101 cgtggctgtg aattgaaact taaatctaaa gctaaagcta ttttattaat taaagcagcc
     2161 acctacgcgt ggctgtgaat tgaaacttta ccgtttaccg attggatttt aatgctagag
     2221 gcagccacct acgcgtggct gtgaattgaa accctaaatt gccgtaaaaa tctgccatta
     2281 gctacgcgca gccacctacg cgtggctgtg aattgaaacc tcataacgaa tccaatcctg
     2341 ttcggttaat ccccgcagcc acctacgcgt ggctgtgaat tgaaacaggg gcgtgtaccg
     2401 caatcaattt taattataac caagcagcca cctacgcgtg gctgtgaatt gaaacctcgt
     2461 cttgaattgg aaaatcaagg gcgacaatag gcagccacct tcgcgtggct gtgtattgaa
     2521 acggtgtacc cacaaatcct atgttttcgc cattggctgc taccttcgca tcaatatata
     2581 ttgaaatatc aatggcaaat ttgtatgttg ttaattagac tgattgcatc tgctatgtat
     2641 catagtaatg acgaaaatat tctattttgc aaaaaataat cagaaattac ccgcttgttc
     2701 gaactgagtt tttgttttct aagatgtgaa atgatacact ttagatagtt ttaaaatata
     2761 gagacagtaa gatgagtagt acggaattat tagtgaatgt taccccaagc gaaacaaggg
     2821 tagcgttggt agaaaacgga atattgaaag aattacatat tgagcgggaa gctaaacgcg
     2881 ggattgttgg taatatttat aagggaaggg tgacaagggt attaccaggt atgcaatctg
     2941 cttttgtgga tatcggcttg gagaaggctg cttttttgca cgcttctgac atcgtttcgc
     3001 atacggaatg tgttgatgaa aatgaaaaga agcaatttct ggttaaagat atttctgaat
     3061 tggtgcgtga agggcaagat atagttgtac aagtagtgaa agatccaatt ggtactaaag
     3121 gagctaggct cactactgat attacgttac catctcgtta tttagtgttt atgccagaaa
     3181 atagccatgt gggagtatct cagcggattg aaagtgaaga agagcgtgct cgtttaaaaa
     3241 gcttggttga gccttattgt gatgaacttg ggggctttat tgtacgtacc gctgcagaag
     3301 aggcaactga agaaagttta aagcaagatg ttgaattttt aaaacggttg tggcgtaaag
     3361 tattagaacg aaaagggaaa tattccgcaa aatcaatgct ttatggagag cttgcgcttg
     3421 ctcaaagggt actgcgtgat tttattggta cacatatcaa ttcggttaga atcgattcaa
     3481 agctaactta tgagcaagtg cgagaatttt tagctgagtt tatgccagaa ttaacggatt
     3541 gtgtcaccct ctataccggt cagcaaactt tatttgatgc ctatggtgta gaaaaggcaa
     3601 tccaaaaggc attggataaa cgggttgatt taaaatcggg gggctattta attattgagc
     3661 aaactgaggc gatgacgact attgatatta atactggtgc ttttgtcgga catcgtaacc
     3721 ttgcacatac gattttcaac acaaatattg aagcaataca ggcaattgca caccagctac
     3781 agttgcgtaa tttaggtggt attatcatta ttgattttat tgatatgcaa gaggaagaac
     3841 atcgtgagcg cgttttacaa tcgttacaag aggcattaaa aggcgatcgt gttaaaacca
     3901 atgtgaatgg ctttactcag cttggcttgg tggagatgac cagaaaacgt acaagggaga
     3961 gtctagagcg aattctttgt tgcgattgcc aagcttgcca agggcgtggc acagtgaaaa
     4021 ctgtagagac ggtttgctat gaaattatgc gtgaaattgt gcgtgtacat catttgttta
     4081 agacagaaaa tattcacgtt tatgcagcta aagatgttgc tgaatattta attaacgagg
     4141 aaagccacgc attattggct gagcttcagg cgtttatcgg taaaaaaatt gagattaaac
     4201 ttgagccata ttatcatcaa gagcagtttg atgtggtcgt gatgtaatct attacttggt
     4261 tgaggctaaa tagttagcct caatatcgtt agttacaaaa aatttagaaa atctgaccgc
     4321 ttgtttccta tccaaattcc tcaaaataac gtataatcgt ccagttttat ttatcctttt
     4381 ttgaaatcta aggtaattca atgtctgaag tagaaaatca agaattagat ctaaacggtg
     4441 aaatgttagc ccgccgtgaa aaattaaaca aaatccgtga gcagggtaac gcatttccaa
     4501 atacattccg tcgtgatgcg ttagccaaag atctgcacgc tcaatatgat gcagtggaag
     4561 gtgaaacctt aaaagaacag aatattcaag tgaaagttgc cggtcgtatt atgttaaaac
     4621 gagtaatggg taaggcatct ttctttacca ttcaagatgt gagcggtgcg attcagcttt
     4681 atgtagctcg tgataactta agtgacagcg tttacgaaga aaaagtcagc ctttgggatt
     4741 taggcgacat tgtgggcgtc gaaggtgcgt tatttaaaac caaaaccggt gaattaacgg
     4801 ttcgttgctc tgaagttcaa cttttaacca agtctttacg cccgttgccg gataaacatc
     4861 acggtttaac ggatttagaa actcgttatc gtcagcgtta tttagacttg atttctaatg
     4921 aagaatctcg ccgtactttt atgatccgct cgcaagtagt ttcaggcatt cgcaaattct
     4981 tcttagaaag agactttatt gaagttgaaa cgccaatgtt gcaagtgatc ccgggcggtg
     5041 cggcagcgaa accgtttatc actcatcata atgcgttaga tgtggatatg tatctgcgta
     5101 ttgctccaga gctttactta aaacgtttag ttgtgggtgg ttttgagcga gtatttgagc
     5161 ttaaccgtaa cttccgtaac gaaggggttt cagtacgcca caatcctgaa tttacaatga
     5221 ttgagtacta ccaagcctat gcggattatc acgatttaat ggataatacc gaagaactgt
     5281 tacgccaatt agccttagat attcttggca caactgacgt accttatggt gattatgtat
     5341 tcgatttcgg taaaccgttt gagcgtatta cgatgcacga tgcgattgtg aaatatggta
     5401 acggcattca acgtgaagat ttagacagct tcgagaaaag tgtggaaatt gcgaaaggct
     5461 taggcattga aattcaaaaa tcttggggtt taggctcggt agtcaatgcg atttttgaag
     5521 aagtggcaga acatcaatta attcagccaa ctttcttgat ggctcacccg gcagaaattt
     5581 caccattagc tcgccgtaat gatgaaaacc cagaggtaac tgatcgtttt gagttattta
     5641 tcggtggtcg tgaaattggt aatggtttct cagaattaaa cgatgcagaa gaccaagcag
     5701 agcgttttga tgctcaagtt gcggcgaaag atgccggtga tgatgaagca atgtttaaag
     5761 atgaagactt tatcgtggca ttggaacacg gtctgccgcc aacagcgggt gaaggtttag
     5821 gaattgaccg cttagtaatg attttcgcta acgccccatc aatccgtgat gtaattttat
     5881 tcccagcgat gcgtcagaag taatcagaaa tagaacaaaa gcacacttcg gtgtgctttt
     5941 ttgtattaca agcggtcaaa tttttaataa aaaatgcaat ttattttgct aatccggctt
     6001 taatcatcgg ttcaaaaaag tcataatcaa ccaaaaaggc atcgtgtccg aaatcggagt
     6061 gaaactcgtg gaactccaaa tttacgcctg cctgttctaa tttttgtttg ctcttataca
     6121 cgtcaacttg cttgaatagt tggtcgcttg ttaccgctac aaaagtgtag ttggctttaa
     6181 tacgttttag ggcggtttct tcattttcat agccaagagc cgggtcgtat aaatctaatg
     6241 ctcggagtaa acggaggtaa gtgttggcat cgaaacgacc taaaaatttc gtgccttgat
     6301 agcttaaata ggattccact tggaaatggt cgccccaaat ttgtccttgc tgtttggttt
     6361 ctcgtccaaa ggctttagca agttgaatat cagtacggta agtcagcata ccgagcatac
     6421 gggcgatttt taagccgtta tcaggtgcaa tgccatcata atagttccca ccgttaaagt
     6481 gtggatcgtt aataatagct tggcgcataa cgtggttaaa accgatagcc tcggcactta
     6541 gggtaagtga cgaacaaaga tttataatgt tgcgaacttt atcagggtaa ctaatacccc
     6601 actgtgttgc ttgcattccg ccgaaagatc cccctaccac tgcgtgaagt tgagggattt
     6661 ctaaatagtc caataatgct ttttgtacat taacaatatc ttgcactgta ataattggaa
     6721 acaaactacc gtaaggttta ttggttttag ggttaatcga agctgggccg gttgtgcctt
     6781 tacaaccgcc taatacatta ctgcaaataa agaaatattt atcggtatca aacgcaagcc
     6841 ctttgccgat gaaatcttgc caccagcctt tctcggttgg tgagtcatag taaggcttag
     6901 catcaccggt aagggcgtgg caaagcaaga ccgcattgct tttctctgca tttaatctgc
     6961 cgtaagtctg gtaagccact gtgatagggg acagttcgcc tcctaatgtg agtggtaaag
     7021 gtacgtcctc aaaaagggta atctgctgag ccataaaaat cctgtttgca aaaaattctg
     7081 cttttttgac cgcttgttgt aatgaccgtt aaattagcgg tgttcatttc aattgtcaag
     7141 aatttttaga cgtctaaacg tctagatttt taaacggcta aaatcaaaat tttattgctg
     7201 agaagatatt tattacttaa caaataacat gaaatagaat aaatttattg aacgattaga
     7261 ctattttgtt tgtctctaat ttacccatct taaatagggt agcaaaagta aaggatctca
     7321 tttgaaccaa acgcgtgata ggttagaaaa attgccactt ttccataaaa tgcagcagtt
     7381 tattattaag gcattgaaaa taattgcaat tttcccctta ttgggaatag ctatattatt
     7441 agtggttgat tcacttacaa gttatattgt gagggataaa atttatacag atagtaaaca
     7501 gattccttac cgggagtatg cagttgtact cggtacggca aaattttact ctaaagatgt
     7561 gattaaccaa tattataaat atcgtttgga agccgctaag caattagtcc aagaaggaaa
     7621 ggtcaatcag cttttgttaa gtggagataa taaaacgccg tactataatg agccaaaaac
     7681 aatgacaagt gatctactta aaatggggat tgcgacttca aaaattaagc aggattatgc
     7741 ggggtatacc actttttatt caataattag ggctaaaaaa gtatataaat taccgccatt
     7801 tacgattatc tcccaaaaat tccactgtga aagggcattg tttattgcaa aatttaggga
     7861 tattgatgca atttgttatg ccgctacata tccagagggg gcttatcagg tacgaatgcg
     7921 ggaaatttta gccagatcgg caatggtttt tagtttgttg ataggtaaag agcctgaatc
     7981 attggaagat atccgttcta aatgagatag ccggtcagat aatgttgacc ggctattttg
     8041 cttataaaat aatggttttg tttttatata caaatacttt gtcagcaagc actaattcta
     8101 aggcttggct aagtacggtt ttctcgacat cacgtccggc acgcatcata gcctctgcgg
     8161 tgtaggtgtg atcaacatta atcacatttt gcataataat cgggccttca tctaattcat
     8221 cattcacaaa atgggcggtt gcaccgataa ttttcactcc acgttcgtat gcacgatgat
     8281 aaggttttgc accaatgaaa gcgggtaaga acgagtggtg aatattgaca actcggtttg
     8341 gataacgagc gacaaattcc ggattcagca cccgcatata tttagccaac acaatataat
     8401 caggtgaata ttcatcaatt ttttccgcta ataatttatc gtgttccact cgggttaaat
     8461 tttcgtggct gactaaatgg aatggaatat cgaaacgttc aactaaactt tttaatgttt
     8521 cgtggtttcc aatcacggca gcaatttcta catctaatcc accatagtaa tttttcatca
     8581 gtaagtcacc taaacaatgt gcttctttgg taactaaaat aacgatacgt tttctttgtt
     8641 tagggagtaa tttatagctt gagccttcag gtaaggtaaa ttttaaatca gtgagtaagg
     8701 tttcatcatt aaaaattccg tttaattcgg tacgcataaa aaaacgttta gtttcattct
     8761 caacaaattc agaattttta ataatattta attggtgctt atagcaaata ttggtaattt
     8821 tagcgacaag tcctgtatca tcaggacat
//
LOCUS       DANLUA010000098         8834 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLUA010000098 DANLUA010000000
VERSION     DANLUA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..657
                     /locus_tag="PXB44_001667"
     CDS             <1..657
                     /locus_tag="PXB44_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5282129.1"
                     /translation="TIVGPNGGGKSTLLKILLKLLSPNKGKVIHQKGLKIGYVPQKLH
                     LEPSMPITVYRFLALKPHTTKEMINQALDLFSITHLTENSMQKLSGGELQRVLLARAI
                     LDRPQLLVLDEPMQGVDIIGQTELYQLLNKTRDLLNCAILMVSHDLNIVMANTDEVLC
                     INKHICCAGSPEVITHDPSFIHFFGDQFARNVAFYSHKHNHHHNLHGDICLGECSCEH
                     "
     gene            822..3635
                     /gene="sucA"
                     /locus_tag="PXB44_001668"
     CDS             822..3635
                     /gene="sucA"
                     /locus_tag="PXB44_001668"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL5282130.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3738..4967
                     /gene="odhB"
                     /locus_tag="PXB44_001669"
     CDS             3738..4967
                     /gene="odhB"
                     /locus_tag="PXB44_001669"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL5282131.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5260..6420
                     /gene="sucC"
                     /locus_tag="PXB44_001670"
     CDS             5260..6420
                     /gene="sucC"
                     /locus_tag="PXB44_001670"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL5282132.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6429..7298
                     /gene="sucD"
                     /locus_tag="PXB44_001671"
     CDS             6429..7298
                     /gene="sucD"
                     /locus_tag="PXB44_001671"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL5282133.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7369..8814)
                     /locus_tag="PXB44_001672"
     CDS             complement(7369..8814)
                     /locus_tag="PXB44_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL5282134.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
BASE COUNT         2759 a         1912 c         1875 g         2288 t
ORIGIN      
        1 acgatagtcg ggccaaatgg tggaggaaaa tctaccctgc ttaaaatttt gctaaaatta
       61 cttagcccaa ataaaggaaa ggttattcat caaaaaggac tcaaaattgg ctatgtacca
      121 caaaaattgc atttagaacc ctcgatgccc attactgttt atcgatttct tgcgctaaaa
      181 ccacatacta ccaaagaaat gattaaccaa gcactagatc tgttttctat tacccatctc
      241 actgaaaata gtatgcaaaa attatcgggt ggtgaacttc aacgtgtatt gctggctcgg
      301 gcaattttag atcgtccgca actcttagtg ttagatgaac caatgcaagg cgttgatatt
      361 ataggccaaa ccgaactcta tcaattactt aataaaacaa gggatctcct taactgtgct
      421 attttaatgg tctcacacga cttaaatatt gtgatggcga atacagatga agtgctttgc
      481 atcaataaac atatttgttg tgccggaagc cccgaagtca ttacacacga tccgagcttt
      541 atccattttt ttggcgacca atttgcacgt aatgttgcat tttattcaca taaacataac
      601 catcaccata atttacatgg cgatatctgc cttggtgaat gtagttgtga gcactgatag
      661 gcgtcctttt taccaaatgt ttaaaatttg taaaaataat gctaaaaata accgcttgca
      721 gcacaagatt tatctaaaaa tcagacttac ttcacatttt atttgcttaa attggagtat
      781 aataagggcg tgatttcata ccacaaacag gtcaggtctt tatgcaatat acacattttg
      841 atgattggct tgcatcatct gcattaggcg gtgcaaacca atcttatata gaagaaattt
      901 atgaacagta tctagaaaat ccagcaaatg ttgatgaaag ctggagaaac atctttgatt
      961 catttcctaa aactccccaa gttgaacaag ctcacacccc tattcgtgac tatttccgta
     1021 aattagcccg tgaacagcaa cctcaatccg tcactattat tgacccagaa gcaggggcga
     1081 aacaagttcg cttactgcaa tggattaacg ctcaccgttt ccgtggctat ttagaggcaa
     1141 aactagaccc aattaactat taccgttgga aaacctccag cgtgcctgaa ttggactaca
     1201 aatatcacgg cttcaccgat gaagatctca acgaaacttt tacggtcggt cgctatgtct
     1261 atgataaaga cacaatgaag ctcagcgaac tggataaagc cctaaaagaa acttatttag
     1321 ggcctattgg tttagaattt atgcacgttc aagatattga acaacgcaac tggcttcaag
     1381 ccaaaatgga aagcgtgcta aacaaaccgc tgttcaccaa agcagaaaaa ctgaatttat
     1441 taagcgaatt gaccgctgcc gatggtttag agcgttactt aggggcaaaa ttcccggggg
     1501 caaaacggtt ctcattggaa ggtagtgatg cctttattcc gatgatgaaa gaaattatcc
     1561 gccacgcttc ccgtcaaggt atgaccgatg tggttttagg tatggcacac cgtggtcgtt
     1621 tgaatatgtt agtgaacgta tttggtaaac gcccggcaga attgtttgat gaatttgccg
     1681 gcaaacacgc tgatgaaagc cgcacgggcg atgtgaaata ccaccaaggt ttctcctcaa
     1741 actttgccgt agatgataaa aacgttcacc ttacccttgc tttcaaccca tcgcatttag
     1801 agattgttag cccggtggta atcggctcag tgcgtgcaag acaagaacgt atgcgtgata
     1861 tcgaacacga aaaggtgctt gcggtgaccg ttcacggtga ctctgcagtg gcagggcaag
     1921 gtgtagtgca agaaacctta aatatggcaa atgttcgtgg ctataaagta ggaggcacta
     1981 tccgtattgt gatcaacaac caaatcggat ttaccacctc taacccaaat gacacccgct
     2041 ccaccgaatt ttgtaccgac attgccaaaa tgattcaggc accgattatt cacgttaatg
     2101 gtgatgatcc ggaagctgtt gcctttgcag cgagaatggc tgtggaatat cgtactaaat
     2161 ttaaacgtga tatttttatt gatttaatct cttaccgtcg tcacggtcat aatgaagccg
     2221 atgagccttt agcaactcag ccaatgatgt atagcatcat caaaaaacac ccgacacctc
     2281 gcaaagtcta tgcggatcgt ttaattaccg aaggtgtttt aaccaacgag caagaaatcg
     2341 agatgttaaa cctgtatcgt gatgccttag acaacggcga atgcgttgtg ccggaatggc
     2401 gtgaaatgga tatggcagcg gtcgattggc tacaatatct caattacgac tggactgccc
     2461 tatacgaaag taaattccct gaagaacgat tcttaaccat tgccaaacgg gtgtgcgaat
     2521 atccggcttc gcttaaagct cattctcggg ttgaaaagat ctacactgat cgcaaagaga
     2581 tgtatgaagg caagaaatta ttcgactggg gtatggctga aacaatggca tacgctactt
     2641 tgttagacga aggcacacat gttcgccttt caggagaaga tgccggacgt ggcactttct
     2701 tccatcgcca tgcggtaatc cataaccaaa atgatggaac aggctatgtg ccacttactc
     2761 aactgcacgc caatcaaggg cgtttccaag tatgggattc cgtactctct gaagaagcgg
     2821 tactcgcctt tgaatatggc tttgccacta ccgacccgaa aacactgacc atttgggaag
     2881 ctcaatttgg agatttcgct aacggagctc aaattgttat cgaccaattt atcagctcag
     2941 gcgaacaaaa atggggcaga atgtgcggtt tagtgatgtt attaccacac ggctatgaag
     3001 gtcaagggcc ggaacactcc tctgcccgag tggaacgcta tttacagctt tgtgctcagc
     3061 aaaatattca agtttgcgtg ccatcaacac cggcacaggt ttaccatatg ttacgccgtc
     3121 aagcgatccg caaaatgcgt cgcccattgg ttgcaatcac accaaaatca ttgctacgcc
     3181 acccacttgc ggtgtcatct cttgatgaat tgattaacgg ttcattccaa aatgtgattg
     3241 gcgagctcga taatatcgat cctaaacaag tcaaacgtgt ggtgatgtgt tcgggtaaag
     3301 tgtactacga cttaattgaa aaacgccgtg aaaataacca aacggacgtt gccattattc
     3361 gtatcgaaca gctctacccg ttcccacttg aagaaatgca acaaattctc gagccttacg
     3421 ctcacgttaa agactttgtc tggtgtcaag aagagccact aaaccaaggt gcgtggtatt
     3481 gcagcaagca caattttgag gcttctctgc cggaagatgc aaaattaact tacgcaggca
     3541 gaccggcttc tgcttcaccg gcggtgggtt atccatcgtt acacgcaaaa caacaaaaac
     3601 aacttgttga agatgcttta acgatcgctg aataataaaa tgggcgtata tacgccctac
     3661 aagcggttca cttttaacca aattttacaa tttacgctgt cggagtttta aaactctctc
     3721 gaaaaaagga ataagttatg accattgaaa ttttaacccc cgatttaccc gaatctgttg
     3781 ccgatgcgac cgttgccact tggcataaaa aagtgggcga caatgtaaaa cgtgatgaaa
     3841 ttttagtcga aattgaaaca gacaaagtcg ttttagaagt accggcttca agcgatggta
     3901 ttcttgcaga aattacccaa gaacaaggtg ctactgttgt atccaagcaa tctctgggta
     3961 aattagtagt agctaaagcc ggtgatatta gctctgcaac tattgagcaa aaaacagaat
     4021 ccacgccatc agaccgcaaa cacgcagcaa ttgaaaacag ccacgcgaac gctgatgatc
     4081 aagggcctgc gattcgccgt ttattagccg aacacgactt acaagccgct gatattcaag
     4141 gcagtggtgt aggcggcaga atcacccgcg aagatattga aaaagagatt gccaaacgag
     4201 tgcaaatcaa ccaagtaaaa gggtctgcaa ccagccaaaa caccgttagc actgtagcat
     4261 ttaacagccg tagcgaaaaa cgtgtgccga tgacacgact ccgcaaacgt attgccgaac
     4321 gcttactaga ggcaaaaaac accactgcaa tgctcactac ttttaacgag ctggatatgc
     4381 aaccaattat gaatttacgc aaaacctatg gcgaaaaatt cgaaaaacaa cacggtgtgc
     4441 gtttaggctt tatgtcgttc tacattaaag ccgtggttga agcattaaaa cgctaccctg
     4501 aaatcaatgc ctccattgat ggcgatgatg tggtttatca taactatttc gacatcagca
     4561 ttgcggtttc taccccacga ggcttagtca cacctgtgtt gcgtgattgc gacaaaatga
     4621 gtatggcaga tattgagaag aaaattaaag aacttgccga aaaagggcgt gacggcaaat
     4681 taaccgttga agacttaacc ggcggtaact ttaccatcac caacggcggg gtattcggtt
     4741 cattaatgtc caccccaatt atcaacccgc cacaaagtgc gattttgggt atgcacgcta
     4801 ttaaagaccg ccctgtcgcc attgacggac aagtagtgat ccgcccgatg atgtacctag
     4861 ccttaagcta tgaccaccgt ttaattgacg gtaaagaatc tgtcggtttc ttagtggcag
     4921 ttaaagagtt attagaagac ccaacccgtc ttcttttgga aatttaatcg aaaatatgac
     4981 cgcttgtact tttagctaca agcggtctgt ttttaataaa atttgacaaa atccgataaa
     5041 cgctctattt gagttccctt ctgagccacc tgagcgactt tgaatttgta ggaaaaattt
     5101 agtgtttgag cttacaaggc aagcgagtta taaatttttc cgtcaagcaa attcaaacta
     5161 agcgaagaaa gtggcgaagc agggtgtgct ttcttttggt tacttttctt tgcacaagca
     5221 aagaaaagta acataaaaaa aaccaaacga ggaaactcta tgaatctaca cgaatatcaa
     5281 gcaaaacaaa tattcaaaca atacgggctt cccgtaagcc aagggattgc ttgcaaaacg
     5341 gcagaggaag ccgttgaggc aattaaacaa ctacaaggtg aacaatgggc gataaagtgc
     5401 caagttcacg ctggtgggcg tggcaaagcc ggcggggtga aactggttcg caatgaagcc
     5461 gaagtgcgtg aatttgccga taaatggctc ggcaaacgct tagttacctt ccaaaccgat
     5521 gctaatgggc aaccggttaa taccttatat ttagaagaaa cctgcaatat cgaccgtgaa
     5581 ttgtacttag gtgcagtggt tgatcgctcc gctcaaaaag tgatttttat ggcatcaagt
     5641 gccggtggta tgaatattga agaagtagcg gagaaaaccc ctgaattaat tcacaaagcg
     5701 gtaattgacc cactcaccgg tgggcagggt tatcaagggc gtgaattagc atttaaatta
     5761 ggcttgaaag gtgatgaagt taaacaattt gcggatattt tcgtgaaact ggctaaccta
     5821 tttgttgaaa aagatttagc tttattagag gtaaatccac ttgtcatcac taaagaaggg
     5881 caactactct gcttagatgc aaaaatgtcg attgacagca atgctctcta ccgccaccca
     5941 gaacttaaag agttgcaaga cccatcgcaa gatgacgaac gtgaagctga agcggaaaaa
     6001 tggaatctca actacgttgc actagatggc aatatcggct gtatggtaaa cggtgcgggg
     6061 ctggcaatgg ggacgatgga tatcgtcaaa ctttatggtg gtaaaccggc taacttctta
     6121 gacgttggcg gtggtgcaac caaagagcgt gtagcagcag cttttaaatt gattttatct
     6181 gatccgaatg tgaaagccgt gttagtcaat atttttggtg gtatcgtccg ctgcgatttg
     6241 attgctgatg gcgtcattgc tgctgtaaac gaagtcggcg tgaatgtgcc tgttattgtg
     6301 cgtttagaag gcactaatgc cgcacagggg cgtgagattt tagccaatag cgggctgagt
     6361 attctagcgg catcatcatt aaaacaagcg gctgaattag cggtaaaatc tgcaaattag
     6421 gaggaaaaat gtcgatttta attgataaaa acacaaaagt aatttgccaa ggtttcaccg
     6481 gcggacaagg tactttccac agcgaacaag cccttgctta tggcacacaa ttagtcggcg
     6541 gagtttcgcc gaataaaggt ggcacaaccc atttaggctt accggttttc aacaccgtac
     6601 gcgaagcggt agaagcaacc ggtgcaactg caaccgttat ctacgttccg gctcccggct
     6661 gtaaagatgc tattttagaa gccattgatg ccggcatcca actaattgtc tgtattactg
     6721 aaggcattcc aaccttggat atgctacaag ttaaacaacg tctaaaccaa accggcgtgc
     6781 gaatgatcgg tccgaactgc ccgggtgtga ttacccctga tgagtgcaaa atcggcatta
     6841 tgccgggcaa tatccacaaa aaaggcaata tcggtattgt ttctcgttcg ggtactttaa
     6901 cctatgaagc agttaaacaa acaactgacg aaggctttgg tcaatctact tgtgtcggta
     6961 tcggtggtga tccgattccg ggatctaact ttattgacat tttaaaactg ttccaagacg
     7021 atcctgaaac tgaagcaatt gtgatgattg gtgaaatcgg cggctctgcc gaagaagaag
     7081 cggcagcttt catcaaagaa aatgtcacca agcctgtagt cagctatatt gccggtgtca
     7141 ccgcaccaaa aggcaaacga atgggacacg ccggtgcgat tatcagcggc ggcaaaggaa
     7201 ccgccgatga aaaattcaaa atccttgaag atgccggtgt gacaaccgtc agaagtttag
     7261 ccgaaatcgg ttcagctctt agaaaaattt taggctaaac taaaagttca agcagtcata
     7321 tttacccatt tttttgtaaa tatgaccgct tgtttttttg ctattaaatt actctttcga
     7381 tttccaaatt aatgggtcaa cacctttatg gataaggtct tgatgttctt caattctaaa
     7441 cactggctct ttgccacttt tcacttggcg aagatagtct tttaaaataa tccagacaat
     7501 cggagcaaga attaagatag ccaccaagtt aattaccgcc attgatgcca tcactgtatc
     7561 ggcaaatgcc catacaatcc ctccgtctct tactgcaccg aagtacacaa agaataaaac
     7621 gatggcacgg aatccccaca caaaccacgg actgttttta atgtagcgga tattagtttc
     7681 tgcataagca tagttaccga taatggatgt gaagcaaaac agtaaaatca caaatgctaa
     7741 aaagtgtaaa ccaaattcac ctaaatggaa ttgtaacgca ctttgagtaa gtgaaatacc
     7801 tcttaaagtt tcaccaccat agttatttga cataagaatg atcactgcag tacaagtaca
     7861 gactacaatt gtatctacaa atacgcccaa catttgaatt aagccttgac ttgccgggtg
     7921 tttcgcatca gaagtcgctg cagaatttgg tgcagatccc ataccagctt cgtttgagaa
     7981 taagccacgt ttaatcccca ttagcatagt tttggatacc aatgctccaa acgttccacc
     8041 tgcagcagca gaaaaatcaa aggcgctttc aataattaaa gagattatac ttggcacttt
     8101 ttcaatattc atacctaaaa taattactgc aataatcaag tagaataatg ccatcattgg
     8161 aacaacttgg gcagaaactt taccaatacg cttgacccct ccagaaataa ttaaccctgt
     8221 aatcaccact aatgtcatgc ctacgtagac tgggttccaa tcccacgcat ttttggttgc
     8281 ttccacaata gagttagatt gcacagcatt aaaagcaaaa ccaaaggtga agataagcgt
     8341 cactgcaaaa gcaatagcaa gagaaggcag ttttaaacca cgagtgatgt aataggctgg
     8401 cccaccacga aacattccgt ttggatcttt ggttttatac aactgagcca gtgaagactc
     8461 agcaaaggcg gaactcattc ccattaaggc ggtaatccac atccagaata ccgcacccgg
     8521 cccacccaaa gcgattgcag tagctacccc accaatatta ccaaccccaa cacggctggc
     8581 taatccagta gtaaaggctt ggaacggagt taatgatttc ccttccgtag aacgtcctcc
     8641 ccacatttca cgtaaacttt gcggaagaag acgcagctgg acaaaacctg ttgtaagagt
     8701 gaaaaataac cctgcaccga gcaataggat aatcgttaca tcccaaagtg gaccgtcgat
     8761 tgtgttcaca atccaagtca gtacactctc aaaaaataga ataattgaat ccataaagac
     8821 ctcttaattg aaaa
//
LOCUS       DANLUA010000099         8827 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLUA010000099 DANLUA010000000
VERSION     DANLUA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            39..386
                     /locus_tag="PXB44_001673"
     CDS             39..386
                     /locus_tag="PXB44_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282135.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
     gene            383..1300
                     /locus_tag="PXB44_001674"
     CDS             383..1300
                     /locus_tag="PXB44_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL5282136.1"
                     /translation="MKSQIQATEQAITALYKNKSETINSLEKQIKNSQKIYQDKQSYL
                     VEIEKSMNDYAELVKRYEKLLKVGHSSHDEVNIQKSRYFQQKSLFNELKQELIQLKST
                     QLSLENEIETRKTEFNNQIIRYEMQKSDLNIRLMEFESVSDIIVNASLDGKIESTSVT
                     VGQIIKENDPLAQILPQNKGNYQLVMWVPNSAISFIKQGDEVNIRYEAFPFEKFGQFK
                     GKITSISTLPASLQELSFYKNLPVNLEQGIPLYKILIELADQNVKYNDTSLYFMSGMK
                     AEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            1300..2091
                     /locus_tag="PXB44_001675"
     CDS             1300..2091
                     /locus_tag="PXB44_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL5282137.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
     gene            2469..3386
                     /locus_tag="PXB44_001676"
                     /pseudo
     CDS             2469..3386
                     /locus_tag="PXB44_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3388..4056)
                     /locus_tag="PXB44_001677"
     CDS             complement(3388..4056)
                     /locus_tag="PXB44_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL5282138.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(4100..5467)
                     /locus_tag="PXB44_001678"
     CDS             complement(4100..5467)
                     /locus_tag="PXB44_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL5282139.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(5586..5681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(5852..6904)
                     /locus_tag="PXB44_001679"
     CDS             complement(5852..6904)
                     /locus_tag="PXB44_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5282140.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(6951..7376)
                     /gene="ruvX"
                     /locus_tag="PXB44_001680"
     CDS             complement(6951..7376)
                     /gene="ruvX"
                     /locus_tag="PXB44_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL5282141.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(7376..7936)
                     /locus_tag="PXB44_001681"
     CDS             complement(7376..7936)
                     /locus_tag="PXB44_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL5282142.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(7936..8667)
                     /gene="rsmE"
                     /locus_tag="PXB44_001682"
     CDS             complement(7936..8667)
                     /gene="rsmE"
                     /locus_tag="PXB44_001682"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL5282143.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
BASE COUNT         2674 a         1678 c         1741 g         2734 t
ORIGIN      
        1 ataaatgtgc taatgtagta gtgtaaaatt ggtattatat gttcagagaa gaagctattg
       61 aatatcaatc gaaaaaatgg aaaagcacag ctgtactatt tagcagagtt ccagctttgg
      121 ttgtcttttc catttctttc tttatcttta ctgcctttat tctatttatt gtgtttggta
      181 attatacccg ccgtgaaacg gttgtgggtg aattagtgat gcaatctcat ccgattttat
      241 tatctgcccc aaaatccggc tatatatccg aaaattatat tcaaccacat cagcaggtaa
      301 aaaaaagggc gatccgctgg ttaaaattac gctggataga attacgcata gcgaaaatat
      361 taatgttaat tctattcttt cgttgaaatc acaaattcaa gcaacagaac aagcgataac
      421 tgctttatat aagaataaat cagaaactat taatagcctt gaaaagcaga tcaaaaacag
      481 tcaaaaaatt tatcaagata agcagagtta tttagtggaa attgaaaaaa gtatgaatga
      541 ctatgcggag ttggttaaac gctatgagaa attattaaaa gtagggcatt ccagccacga
      601 tgaagtgaat attcagaaaa gccgttattt ccaacaaaaa tcactgttta atgagctaaa
      661 gcaagaatta attcaattaa aatccaccca acttagcttg gaaaatgaaa ttgaaacccg
      721 taaaaccgaa tttaataatc agattattcg ctatgaaatg caaaaaagcg atttaaacat
      781 tcgtttaatg gaatttgaat ctgtttcaga tattattgtt aatgcctctt tagatggaaa
      841 aattgaaagt accagcgtaa cagttgggca aattattaaa gaaaatgacc cgcttgcaca
      901 aattctgcca cagaataaag gaaattatca gctagtgatg tgggtaccga atagtgccat
      961 ttcatttatt aaacaaggcg atgaggtgaa tattcgctat gaggcttttc cgtttgaaaa
     1021 attcgggcag tttaagggca aaattacgtc aatttcaacc ttaccagctt ctttgcaaga
     1081 acttagtttt tataaaaatc tccctgtaaa tttagagcaa ggtattccgc tttataaaat
     1141 tctgattgag cttgccgatc aaaacgtaaa atataatgat acctcccttt attttatgag
     1201 tggaatgaaa gccgaagcca cgttattttt agaaaaacgt aaactgtatg agtggatatt
     1261 tttccctatg tatcaattac gcaaaaatat ggagcaataa tgacaggaat taagcagtta
     1321 aaatttgatt ttcggagaag aatacctgtt attttgcaaa ctgaaacagc tgaatgtggc
     1381 ttagcctgtt tagcgatgat gttgggctat tacgataaaa acaccaattt atttgaattg
     1441 cgaagtcaat atggtacttc ctctcgtggc gtaacgctgc atactttaat ttctattgca
     1501 aatgactgtg gtttaataac tcgccctctt tctctagagt tagatgaagt accacaactt
     1561 cgtctgcctt gtattcttca ttgggatttt aatcattttg tagtattaac tcacgcaagt
     1621 gaaaaacagt ttattatcca cgatcctgct ttcggtaaac gtaaattatc ccgtgctgaa
     1681 ttttccaacc attttaccgg agtcgcttta gaagtttggt ctgaagttaa atttgagaaa
     1741 agcaacaatg aaaataccat tagtttgtat gaaaccttaa agcatatttc tggcataaaa
     1801 ggtgcattag tgaaaatttt tgccctttca atgctcattg aactcattgc cttattgatg
     1861 ccaattggaa cacagttagt gatggatcac gttatgcaag cgaaagatca atcattactt
     1921 ttaattatct gtattgggct atttttattt accctttttc gcacaatggt cagtatgttt
     1981 agggcttggg tttccttgaa aatgaattat ttaattgatt ttcaatggac agccagtttc
     2041 ttttctcatc tcttaaaatt accgcttgat ttctttgaaa acgccaagta ggtgatattc
     2101 attcccgatt tagttcatta aaaaccattc agaaaactct aacttcaagc attattacca
     2161 cgattattga tacgattatg ataatcagtt taattattat gatggtgtta tatggcggtt
     2221 ggctcttttg ggtagtatta gctttttcct ttatctattt tattcttcga atggttactt
     2281 atttagttta tcgtcaactg aatgaagagc agattattaa aggagcaaaa gcccattcgc
     2341 attttatgga aactttatat ggtattacaa cgttaaaatc attaggattg gaaaataaac
     2401 gacaagaaca gtggatgtcg ctcaatgcgg atgcttttaa cacatcaatt aaggtaacta
     2461 aatttgatat gctgttttcc ggtattcata cctttatttc taccatcgaa cagattttaa
     2521 ttctttggat tggggcgaca atgatgattg aaaatgtgat gacattagga atgtttgtcg
     2581 cttttaatgc ctatcgaggc tctttctctt cacgaatggg gaatttaatt gatattattt
     2641 ttagcctaaa aattctttca ttacatagag agcggattgc agacattgct ttaaatgaag
     2701 tcgaagatga aagtaaatat caagaactca gatttgaaca taaccaaaca gatatagaag
     2761 ttaaagacct ttgtttcaaa tacgatcctt tttctaaaaa tgtgcttgaa aatgtcaatt
     2821 tagtgatcag aagtggggaa agtgttgcta ttaccgcacc atcaggttat ggcaaaacaa
     2881 cgttattaaa attaattgca ggtttattaa aaccaacctc aggttctgtg tattttaacg
     2941 gcattgatat ttatcagtta ggattaagtc aatatcggag ccaaattgct tgcgtattac
     3001 aggaagataa attcttttct ggttccattt tagagaatat tgttagtttt gaaagtaatt
     3061 atgatcgaga atgggctatc gaatgtagca aacttgcgaa tattcacaat gatattatgg
     3121 caatgccgat gaattatgaa accttattag gagaattagg aaataatttg tctggcggac
     3181 aaagataacg attgtttatt gctcgagctt tatataaaaa gcctaaaatt cttttgatgg
     3241 atgaggcgac tagccattta gatgaagaaa atgagaagct tgtcaatcaa gctatttcac
     3301 gaatgaagat aacaaggatt attattgctc atagacaatc gactattcag tcggcggata
     3361 gaattattgc cctgaatgac aggtaaatta attcctattt ttctgttctt gccgataaag
     3421 tttattgcac ggaatgctgt cgttgttgcc gtctagctct ttccagccgc attgttgaaa
     3481 gtagcgtttt gcctgtgcgt aattgccgat ttggttgcag cttaatttaa tttggcaatc
     3541 aatcgttggt ggctggtttg caatattttc cggttttttg accgcttgtg gtaaggttga
     3601 ggtgtgtgtt tttcgccatt ggctcgggtt aatcgggaat ttgtcctgcc ataagccgat
     3661 tttagcttgc tgtgcttgat cttgtgcttc gatatattcc cgctttgtgg tgtaagccca
     3721 cgccataccg ttttccacta aggctaagtt gatgttttta tcgtttaagt agagggtggc
     3781 aagtaatcgt tggtaacgat catagcctga aactatggca gtaatgtttt gttgaaacac
     3841 gaattgagcc agagcttgtt tggctttgtt gccgtaaggc tgcgcttgtt cgggggcatc
     3901 aatatgttgt agccttattt ttagtggtgt tttattgacc aaacaagtga gggtgtcgcc
     3961 atcgctaatg ccaacgactt tgcattgtag ttggcgttcg gcggcatata ccgatgagaa
     4021 aaataagcta aaaatcaggg cgtaaagata tttcattatt aaaaaagaga gggctaataa
     4081 gccctcattc tatcattttt tattcggctt tacctgtttt aaggcgagcg aagaaatctt
     4141 ttgtttcact aataatcact ttgcgtaacg caatcaatgc gattaaattc gggaatgcca
     4201 ttaagccgtt cacgatgtct gccagcgtcc aaattgtatc gagttgtaaa aatggagcag
     4261 aggcgatgag tgcaataaac agcaagcggt ataatttaat gtattgggtg cgacctcgag
     4321 tgagatagac aaaacagcgt tcaccgtagt agcaccagcc taaaatggtg gtaaaggcaa
     4381 agaagactaa accaactgtc acaattatcg caccgaatga gctgtttaaa ccttgagtaa
     4441 aagcggttgt ggtgagttct gcgcctttta catcgcctgc ccaagcccct gaaatcacaa
     4501 ttactaagcc ggtcattgtg cagacgataa tagtgtctaa gaacgtgccg gtcattgaaa
     4561 ttaggccttg acgtaccggc tctttggttt gggcggcagc ggcggcgatg ggggcagagc
     4621 ctaatcctga ttcattcgag aaaataccac gtgcaacccc taattgaatc gcttgcatga
     4681 cggtgaatcc gactgctccg cctaatgccg cttgtgggtt gaaagctgcg tgtacaatag
     4741 taataattgc ttgtgggact ttttccatat tcagtagaat gacggataaa gcggcgatga
     4801 catacccaat agccataaac ggtacaacaa tggttgcaat tttagaaatc cgctgcacgc
     4861 cacctaaaat aatagcggta acgataaggg ttaaaatgat cgcactgaag ataatcggca
     4921 catcaaaggt gctttctaag gcgtgggtaa tgccattcac ttgggggaac gtgccaatac
     4981 caagcaaagc gaccaatacg ccaaaaaacg caaaggcttt agcaagccaa cgccattttt
     5041 ttcccattcc cagttcgata tagtacatcg gaccaccagc cacaaatccg gctctatctt
     5101 tagttcgata tttaattgcc aataagcctt cggcatattt cgtagccata ccgaataagg
     5161 caatcagcca catccaaaat aatgctccag gcccgcccga atgaatagcg gttgccacgc
     5221 cgacaatatt ccccgtgccg atagtagctg ctagggcagt ggaaagggct gcaaaagcgg
     5281 aaatatcgcc cgattgccct ttgccttttt ccggtttgaa catatagcga aaggcttgcc
     5341 ctagttggct taattgaata cctttgagtg aaaaggttaa atataaccct acgcctgaaa
     5401 ttaaaatcag gagtggcggg ccccaaacaa aactgttgat ttgattaaga atagtgtcga
     5461 tagacatttg atgttcctcg taaaaactgc ttacaaggaa agaaaaggaa tgcaagagac
     5521 aagcggtcaa aaaacggaaa ttttttgcaa agaattagca aaaaaacacc gcttgtatcg
     5581 tataactttt ctcccctgtc cttttgcctg agcgtttcac acattgctgt attttcgcct
     5641 tcggcggcgg attttaatcc gctctctcca agggttcgtc cagtaacagt ccccgcagtg
     5701 ttcactgcac ctgaaagagt ttacctcgtc ggtgaaatgt taattcattt ctctccagct
     5761 accttcatcc gaacaatcta atttatttta taatgtaaaa taaaaaagat ttgccaaatt
     5821 ctagcaactc ttttgcttcc gtcaagtgaa tttaacgata tttttgttct ttcggtactt
     5881 cttctaaatc gctaaataat acggcatatt tttgggtggc tttattggat tctaatgcca
     5941 ctttacacgc catcgtaagt ggaacagaaa gtaacattcc tacggtgcca agtaaccacc
     6001 cccaaaatag aagtgagagg aacactacaa gggtagaaag cccgagtttc ttgcccatta
     6061 atttaggttc aagcgcatta cccacaacca tattaatcac gacaatgccg attaaagtgc
     6121 ctaatcctat agaaaagcca ttgagtacca aggcttgaat cacaattgga attcccgcca
     6181 aaatagagcc gatattcggg acgtagttaa gcaggaaggt tagaattgcc cataaaatcg
     6241 catattctac gcctagaatt tctagtaaga tccaaactaa ccctccggtg agtaggctca
     6301 caaaggtttt aactccaagg taaccaatca ccccttcaag tacttggttg atatactctt
     6361 tttcgttgct taaatcatca tctgggctaa gtgcgagggc taatttatat ttggcaaccg
     6421 gcatttccaa tagcataaaa atgacgacta agaaaatcac aaaaatattc gataatgcac
     6481 cggatagccc cattagcagt ttgctgacca aattcattat tgagcttggg tcaaatttgc
     6541 tcataatctc ttcttgtgag agggtaagtg gaatatgcca tctatgggcg agttctagaa
     6601 tcacctggat tctagatgaa agctgaattt tgtattgcgg aatagcatta gtaaattctt
     6661 gcaaggtgtt attaataatg cctgctaaga aactgaatcc taagataaaa acaataagta
     6721 aaatggtaat ggctaaccat aatggcactt tacgcttagt cataaatttg atcattggtg
     6781 aacaaataat ggcaataaat agcgaaagta aaaatggcac aacaatttca ttggataatt
     6841 taatccctgc cagaataatg ataatggagg ctatcgccac gagagttttg gtggaatgtg
     6901 tcataatttt tccttttaca agcggttaaa aatcagcaat tttttacaaa ttagtgattc
     6961 tcaaaccaac tttctaaaat taaacaagcg gaaatggaat ccactttatc cttttttaat
     7021 gctttgtaac caccacgaga aaaaatttcc gcttttgctt caacagtagt gaggcgttca
     7081 tcttgtagct caaccggcag attaaagcgt ccgtttaagc gattggcaaa ttttctggca
     7141 cgctgagtta aaggctgctc tgtgccgtcc atattcaagg gtagcccaac aaccagcata
     7201 tctggtttcc aatcttgcaa gagttttccg atctcttccc agtttggaat accgtcttgg
     7261 gctttaaatg tagttaaacc ttgagctgtg ccggtaatac tttgtccaac tgcacagccg
     7321 atgctatttg tgccaaaatc aaaggcaatc acggtttttg ttgtggtttc tgtcattaag
     7381 ctctcccagc tcttgacggt atgcttgaaa gccccatcat ttcataggct tctgcccaac
     7441 gctctaaata accggtttca aacagcgttt tttcattcgc atcagacaca atccaataat
     7501 tttgagcaat ttcttgctca agctggctgc cacgccaagt ggaacaacct aaacacacaa
     7561 taaatttttc aggtgaagcc agtgtgccaa aggagtctaa cacatcaccg gaggtagtga
     7621 gcgtaatttt atcggtgatc ggataagtgt gaataaaatc attggcggtt ttggtgtgaa
     7681 taataaagcc acgatcttga tttaccggcc caccgcttaa caccatttgg tcttttttgt
     7741 aatcacgctg gttcgccatt tggaaatcca ttcgggtaat cagctctaga accgacagat
     7801 cggtaggagt gttaattact actcccattg caccttgcgg attatgttcg caaatataga
     7861 ccaccgtacg ctcgaaatag tcatcgtcca tttccggcgt ggcaattaaa aatttacctt
     7921 gtaaattctc taacattaaa tatctccaaa acaaacttgt aacgcagtaa ttgccgcaag
     7981 tgaggcggtt tctgtgcgta acactcgttt acccagtaat acttcagtaa aaccttcgct
     8041 ctcggtcatg gcgatttctt cggcagataa tccgccctca gagccaatca gtaaacgaac
     8101 accagctttc ggcacattcg gcagttcacg aatggtatat ttggctcttg ggtgcaaatt
     8161 cagtttcaac atatcgtctt gttctttgca ccagtcggtg agtttcataa tcgggcgaat
     8221 ttcagggatt tcatttcgtc cgcattgttc acaggcggca atcgcaattt tctgccattg
     8281 ctgtaatttt ttatcttgcc gttcatcatt gagtttcacg ccacaacgtt ccgaccaaag
     8341 tggggtaata gtttttaccc ccagctccac cgatttttga atggtaaatt ccattctatc
     8401 gcctcgggaa atcacttgcc ctaaatggat cggcaggttg gattctcggt catctaattc
     8461 acttgaatca atttttgcaa taatttgctt tttttcgacc gcttgtaacg tagcgtggaa
     8521 aacgtggttt gaaccatcaa ataaaatgat ttgctctcct tgtttcattc gtagcactcg
     8581 cccaacgtgg ttagcggcat cttcacttaa cacgcaactt tgaagaccag ctaacggctg
     8641 tggttggtaa attcttggga ttctcataat tatctagtta taaattaaag gcagacgatt
     8701 gtctgccata ggtaagttaa aacgggttaa ggataaagtc ttgaacaatg cacaatagta
     8761 ataatcgaaa tttcattctc tttaacttca cacactattc tatagccgtt atgatatagt
     8821 tctctcg
//
LOCUS       DANLUA010000100         8800 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLUA010000100 DANLUA010000000
VERSION     DANLUA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(50..823)
                     /locus_tag="PXB44_001683"
     CDS             complement(50..823)
                     /locus_tag="PXB44_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5282144.1"
                     /translation="MNLSNALKLIKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNA
                     EQDVLWLSEIEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENV
                     GLVFALENKAKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCF
                     DKLTDQEVEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWH
                     NTVSAMTETAYQNMVCVETARINTLLQQNETLGVRVLVR"
     gene            972..5168
                     /locus_tag="PXB44_001684"
     CDS             972..5168
                     /locus_tag="PXB44_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5282145.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTN
                     VPTLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSR
                     RLGYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLI
                     KASAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQS
                     DKFNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEF
                     NEGVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGAN
                     IGHQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLD
                     DWKVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGF
                     INGNEIKKQHFAGVKLGYSW"
     gene            complement(5223..6317)
                     /gene="mobA"
                     /locus_tag="PXB44_001685"
     CDS             complement(5223..6317)
                     /gene="mobA"
                     /locus_tag="PXB44_001685"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5282146.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            6404..6673
                     /locus_tag="PXB44_001686"
     CDS             6404..6673
                     /locus_tag="PXB44_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL5282147.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            6737..7375
                     /locus_tag="PXB44_001687"
     CDS             6737..7375
                     /locus_tag="PXB44_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5282148.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            7459..8235
                     /locus_tag="PXB44_001688"
     CDS             7459..8235
                     /locus_tag="PXB44_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5282149.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            8245..8499
                     /locus_tag="PXB44_001689"
     CDS             8245..8499
                     /locus_tag="PXB44_001689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL5282150.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            8501..8794
                     /locus_tag="PXB44_001690"
     CDS             8501..8794
                     /locus_tag="PXB44_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5282151.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2911 a         1758 c         1626 g         2505 t
ORIGIN      
        1 taaatttgta aacaatatta ctaattcagc tgcttgctct cattaaacac tatcgcacta
       61 aaaccctcac tcctagcgtt tcattttgtt gaagtagagt atttatccga gcggtttcca
      121 cacataccat attttgatac gccgtttctg tcatcgcact aacggtatta tgccaaggat
      181 tccaaagtac ggtttcggtc gcattaatat gctcaacttt aatggtgcgc tgatttgcaa
      241 aatcttgaat aaaattcacc gcttgtgccg gataaataca atccacattt tcacttatct
      301 tgcgaggtga tggcacttca acttcttgat cggttagctt atcaaaacag cgagttggca
      361 agccttgcac ttccactttt tcaatatcac caatattaaa ataagagtgc aatgccagtt
      421 gtgctggttc tggatctaaa tgggtaaaat ataactcaca agtttcgcct aacatcattt
      481 ccattttcgc tttattctct aaggcaaata ccaaaccgac attttcatca gaacaagtat
      541 gctcaactaa acgccattct tgaatgcgag ctgtgccgtg ggcaggctct tttattgaac
      601 cgaaccacgg ataacaaatt ggtataccgc cacgaatcgc agagccaatt ctaaacggtt
      661 ctatctcact taaccacagc acatcttgct ctgcattata cggctgccaa cttagcaatt
      721 gtgctccttg taatgcaatt ttagctttga atttattgtg attaacttct agcacgtcta
      781 attcattata tttgattaat tttaatgcat tactcaagtt cataatttat tcctttgatg
      841 actaaaataa ttgacagcaa acactactta gtaattttat ataaaataag ggaagacatc
      901 ttgccaaact atcagttata agacagaata gccagaattt tttattactt ataaggataa
      961 acttctcatt tatgaagacc aaaacattta ctcgttctta tcttgcttct tttgtaacaa
     1021 tcgtattaag tttacctgct gtagcatctg ttgtacgtaa tgatgtggac tatcaatact
     1081 tccgcgattt tgccgaaaat aaaggaccat tttcagttgg ttcaatgaat attgatatta
     1141 aagacaacaa tggacaactt gtaggcacga tgcttcataa tttaccaatg gttgatttta
     1201 gtgctatggt aagaggtgga tattctactt taattgcacc acaatattta gttagtgttg
     1261 cacataatac tggatataaa aatgttcaat ttggtgctgc aggttataac cctgattcac
     1321 atcactatac ttataaaatt gttgaccgca atgattatga aaaggttcaa ggagggttgc
     1381 acccagacta tcatactcct cgattaaata aattagtaac agaagttgtg cctgccgcag
     1441 tcaccaatgc aggtacatct attaaaccct acttaaatga agaacgcttc cctatgtttc
     1501 ttcgtgctgg ttcagggaca caagcgctaa gaggaaaaga aagtaataaa acaactggaa
     1561 tcgctggtgc ttatgaatat cttactggcg gtaccacatt acaattatct aaaagctccc
     1621 ctgatcactg gttagattat tcaagtaacc tttatcaagt aagctatgga ccactttcaa
     1681 cctatgcact acctggtgat agtggttcag gttcttacgc ctatgatatg aacgaaaaac
     1741 gatgggtatt agttggtgtg ctcaatttct ataatggtat ggataatcaa ttcaaccgct
     1801 ctgcgattat ccgtaaagat ttccacgaga aaaaatttgc cgaagatatt gcaggaacaa
     1861 tcaataatac cgtacaaaat gcacaattca attggactgc tcaaggtaaa tccagctctc
     1921 ttagtcaatc aaataatgtg caaaaactca acgttgatct aaaagatagt agcattgcaa
     1981 accaaaacac ttctctgcca caagaaaatc acggtaaaac cattaatttt aatggtaaag
     2041 atgcaactat tgtactaaaa caggatattg accaaggtgc aggtgcatta aatctgaacg
     2101 ctaatctcac tattcgtcct gaaacagacc aaacttggca aggtgcaggt attatcgtcg
     2161 gtaaagataa aaaagtgaat tggcaagtaa aaaatccaca aggcgatcgt ttatctaaac
     2221 tcggggaagg aacactctat gtaaatggac gtggacagaa tcttggcgat atcagtgtag
     2281 gtgatggtat tgtaatactt aaccagcaag ccgatcacca aggaagaaaa caggccttta
     2341 atacagtagg aatcgtaagt ggtcgcccca ctgttgtgct aggtagtgca gatcaagtta
     2401 atcccgataa tatttacttt ggatttcgcg gaggtcgttt agacctaaac ggtaacagca
     2461 tcgcctttaa acgtattcaa aacagcgata aacatgctcg tattgtaaac cacaatcgcg
     2521 atcacatttc taccttaata atacaaggcc aagatcctct cactagtaat gatcttatat
     2581 ggggaaaatg ggcaagtaat agcccagcag acatttacga atataccaat ccttatcaaa
     2641 ataaacgcaa agattacttc cgtctgaaag gtaattcgag agtatattat ccaacgaatg
     2701 ctacaagtaa cgatcactgg gaatttcttt ccagtaaccg cgagcaagca atacagaaaa
     2761 tcctagatgc caaaaactta agacagcgct atgacacgtt taatggtttt ataggggaag
     2821 atgcttccaa taaaactaat gggatattaa atgtcgtgtt tgatacaaaa acagaagtaa
     2881 atacagaaca agataaatta aagaatatct acacaatgtc gggaggattt aaccttaatg
     2941 gtgaactcac ccttaaaggt ggtacattgt tgctttctgg tcacccaacg ccacacgctt
     3001 atgatattaa gaataagcat gatgttgtgc gtgaaaacga ttggcaagac agccatttta
     3061 ctgctaaaaa tatcacggta aataaaatgg cacaactcta tatcgggaga aatgtcaatg
     3121 aagtaaatag tcactttact gcgactgata aagccaaact caatttagga tttattaatc
     3181 gttcaacgcc aagttgctat gattctgaat acacaggcac tacacattgt gaagtgcaag
     3241 cggtcatttc cgataatatt tttgcaaatc tagcaacaac cgccattaaa ggtaatgtta
     3301 aattacaaaa ccatagccaa ttaaatttag gcaaagcaaa cctcactggt tctgtacaag
     3361 ctgatcaaac aactcatatc actttagcaa atcacagtca ctggttaaac aatggtacga
     3421 gccagattgg gcatcttaca atggaaaaag ggtcgatcct tagcctaaac gataaatttg
     3481 ctaccacgga aatcccagtc cgattcaaca agatgatcat ccaaggtaat ctaaaaggta
     3541 atggacgaat taactatacc gcaaatttag ccaagggcga atctgatcat ctccaagttg
     3601 acggtattgc tgaaggaaat tttgtccttg ccgttagaaa tagcacaact gaagcaaatc
     3661 caaaaagctc attaaaccta ctaagcttaa aaaatagcaa ccaagaaggc aataaagctt
     3721 ctatttctct agaaaataat tatgttgatc taggtactta tcgttatgta ttagaaaatc
     3781 gtaatcacaa ttaccattta tttaatccat taataccaaa ttcaacctct aaagagatga
     3841 atgctacatc tgtatcctct attccaaaaa aggaatctgt tactaatgtt cctactttag
     3901 ataagaaaga aactgaacaa aatcttactc aactacaaaa agatttttca gcacaccaat
     3961 tagaaaatca aaaagcaaaa caatctatga taaatgctca atctgagcta agacgactca
     4021 attcacaact gaatgtattg caaaaatatg tgaattctcg tcgcttaggt tactatactc
     4081 agcaggcagt tttagaacaa attagcatta ttcaaaataa aattaaacaa acacaaacaa
     4141 tatttaatga cgctaatgca actgtaaaac tcacagatca aaagctagaa gaagccaaat
     4201 tagctctagg ctctgtaaac gatcttgtat taataaaagc ctctgctcca gcaatgcaag
     4261 caactaatca agatacgagt atgatgaata ttattcaagc agattggata agccaatacg
     4321 ctaacacagc actttctgaa ctctcggcac aggctaattc tgctctgcaa atcagtaata
     4381 gcttagatcg ccaactcttc aaacaaagcg ataaattcaa cgtatggagc agcgtcgaac
     4441 atcagaaaac cgagcataaa tcagatttat accgcccgta taaacaacaa accaacctga
     4501 cccaactggg catacaaatg ccgatagata acggtttaat gtttggagtt gcattatcta
     4561 aaaaccacgc taacgcggaa tttaacgagg gtgtaaacgg taaatcgaat ctactaatgg
     4621 caagcctata tggtaagtgg caatctcaac aaggcacttt tatcagcctt gatggcagct
     4681 acggtaaagc aaaaaaccaa ctctacctat ttggtgaaaa ccactttacc cgccgaattt
     4741 cctctattgg tgctaacatt ggacatcaat ttgacctcgc aggagttcaa attcagccaa
     4801 caataggagc aagatactac catttcagcg gccaagacta tacactagga ggagcgaaaa
     4861 tcagctcacc aaatacccac tttatgacat atcaagcggg tctaaaagct agtaaaacct
     4921 tttcattgga tgactggaaa gttgaaccaa gcattacaac ccactatgtg gatgcaagta
     4981 acaaacgctt agcagtaaac gtcaataata acaatatcaa ccaacgtttc ggtcgctatc
     5041 tgaaaaccga agctggtatc cgatttgaac ataaccaatg gcaattcgac ttacacgctg
     5101 gtttcattaa cggcaacgaa atcaaaaaac aacattttgc tggtgttaag ctaggatata
     5161 gctggtaaga aaaagcccct agaactattt ctaggggctt tttctactct taaaaagagt
     5221 atctaacttg ccttattttc taaccaattc ttaataaaat ctgctattcc cccaatatta
     5281 ttaatatcca accaattttc tctctcaagc gggtaatccg ttgcggtggc aatcgtccat
     5341 tggtctagct ctggcaatgg tttttcaatc cctttgcggt ggagctgaat ttttggcaag
     5401 gtttcgtgtt taaagccttc tacgaaaacg agatcaacct cttgcgggtt gaattttgca
     5461 attaattgag aaaattcgac cgcttgtttg gtttctgtca ttaatgccca acgttcatcg
     5521 cacacaatca ttgtcgggtt tgctcctgct tcacgtaggc ggtagctgtc tttgcctttt
     5581 ttatctacat caacgttatg gtgggaatgt ttaatcagcc caacccgaat accgcaagcg
     5641 gtcaattttg ggattaattt ttccaataag gtggttttac ctgtgccgct gtagcctgta
     5701 attgcaaggt tttttacagg gaattggctt actcggctta aatcttccaa tgtattcacg
     5761 ttggtgaatg ccgatttttg ttcggaaaaa tcaactgcca cgcttttttg tgattggaag
     5821 aacgcaagca attgctgttg gtttgagcct aaataggctt tcaaatcttc ttgtaccgaa
     5881 cgatgaatca acgcaaaagt gggatgtgga cgctcaccat catgggcata tgcaatttgt
     5941 acgttattta tccgcaaagc agtttttaat ttttgcaata acagctcagg cataaagggc
     6001 gtatcgcaag gcacaaaaag caaatagtca ctttcaattt gctcaaatcc tgccagcatt
     6061 ccactgagtg gaccttgaaa atcaggcaag ctatcttgat agtaagaaaa agcaggataa
     6121 agctgctgat attgctcaat agaacgattg acattaagcc aaatgtcact aacttgtggt
     6181 gaaagtcggg ctaaaatgtg tgaaataagc ggttttcctt caaaaagttg taaccctttt
     6241 tctaccccat tcattcgacg agctaaaccg cccgctaaaa tcacggctga aatgcgttct
     6301 tctattgttt tactcacagc tttatccctt aaaattttgc aaaaataaga gtaaaagtat
     6361 attatttcgt ttaatttcca acaagttttt taaggggtaa aagatgaaat gcgaacgttt
     6421 agaagagtta ttagagttac ttggcgaaca ttggcgtaaa aatcctgact tacacctcat
     6481 tgatattttg cagcagcttt cagttgaagt gggcgagcct gataatttca aagcgttaag
     6541 cgatgaagtg ttaatctatc agcttaaaat gcgaaatgca ggcaaatttg agcctattcc
     6601 cggcataaaa aaagattatg aagatgattt taaaacggct ttattgcgag ctcgtggaat
     6661 tttaaacgat taagattgtc ttatccgtta gctagtttag catttattat ttatcaacat
     6721 ttttgaagga atcattatga aaaaattggc attaaaaacg gcatttgttg cactttctgc
     6781 cctttttaca tttaactcaa cggcaatcgc tgctgatcca accgcaggca aagaatatat
     6841 cgaagtgcgt aaagcacctt ctgcacaaaa agaagtggtg gaatttttct cattctactg
     6901 cccacactgt tatgattttg aattaagcta caaaatccca agccaaatca aagaaaaatt
     6961 accaagcgat agcaaattag tgcaatatca cgttaatttc ttaggtcgcc aatctgaaga
     7021 tttaactcgt gcttgggcat tagcaatggc attaggtgcg gaagataaag taaaaaccgc
     7081 attatttgaa ggtgctcaaa aagacgcatt caaatcaatg gacgatattc gctctgtatt
     7141 cttagcaaac ggcattacag ctgaacagtt tgatagcggt attaatagct ttgcggtaaa
     7201 tggcttagtg aataaacaag ttcaattagc ggaagacttc caaattcgtg gtgtgcctgc
     7261 attcttcgta aatggtcaat accaactcaa cttagaaggc tttgcagatt cttcatcaac
     7321 gaatgatttc atcaaacgtt atgttgatgc ggttgtgttc ttaagcaaga aataacccct
     7381 taaaatagtt agggttttta caatgctgag taggtaaatc ctattcagca ttttttatat
     7441 gcaaactcgg agaacaaaat gacaaagtta tctatcctaa accttgtacc tattcgtgaa
     7501 gggcaaactg ccaaacaagc ggtcgattct atggtgaaat tagcaaaaca cgcggaaagt
     7561 atcggcattg aacgctattg gattgcagag caccacaata tgaaaaacct tgccagctcg
     7621 gcaacacagc tcctcattca gcatgccctt tctcacactg aaaaattaag agtgggatca
     7681 ggtggcgtga tgttgcctaa ccatagtcct tatattgtgg cagagcaata cggtacgctt
     7741 gaaacactct accctaaccg tgtagaactt gggcttgggc gagcaccagg caccgatatg
     7801 cgaaccgcta ccgctttacg ccgtaatgca caaagtctgc catttcctga tgaaatcagc
     7861 gaactaaaag gttattttga aggcacaaat tctgtgtctg cctaccctgc acaggggttg
     7921 aatgtgccgt tttatatttt aggctcaagc cctgaaagtg cctatttagc agcagaacgt
     7981 ggcttgcctt atgcctttgc ttcacatttc gctcctcgtt ttatggacga tgcggtgcga
     8041 atttatcgca cacactttaa gccgtctgaa gtgttagata aaccctatgt gattttaggt
     8101 gtgaatgcga ttgcggcaga aaccgaccaa gaggcagaac agcttgctac cactcagact
     8161 cagtttttct taaatgttgt taccaatgcc caaaactatt tacaaccgcc cgtcgaatcc
     8221 gatgataaag tgtgaaaaaa ttttgtgaaa gcggaaatcg taccgcattt cgggcctgtt
     8281 tcgttccaac aaaatgcgtt atacaaccaa gagaaacccg ttgttcgcca aatgactgct
     8341 tgttcactta tcggtagccg agagagcatt gaacaacagc tcaagtcatt aaaagcacgc
     8401 attgaaattg atgaaattat ggcggtaagc tacattttcg atgagcaaaa acagcatcaa
     8461 tcctatactt ggttaaaaga gattgcagat aaggtgtgat atgagcaaaa caatgcaaca
     8521 aaaagccgct gatgcacata atatgtgtaa gctaaaaggc gactctatgt tagataactc
     8581 agaccgccaa attgcctttg aagcagtgta tgatagtgaa gaaatagctg tacaagcggt
     8641 cgaatttttc acacaaaaag caaaatcggt agaaaccgag ccttgcgaca ttcaaagtga
     8701 aatcacaccc attatagatg gctttctgat gaaaatgtgg ataactttca gttgccaagc
     8761 agaagtaatc ttatttcaaa tggcggtgag atagatataa
//
LOCUS       DANLUA010000101         8772 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLUA010000101 DANLUA010000000
VERSION     DANLUA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            12..1073
                     /locus_tag="PXB44_001691"
     CDS             12..1073
                     /locus_tag="PXB44_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5282152.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQCIE"
     gene            1225..2877
                     /locus_tag="PXB44_001692"
     CDS             1225..2877
                     /locus_tag="PXB44_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL5282153.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            2930..3864
                     /locus_tag="PXB44_001693"
                     /pseudo
     CDS             2930..3864
                     /locus_tag="PXB44_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(3886..5967)
                     /gene="recG"
                     /locus_tag="PXB44_001694"
     CDS             complement(3886..5967)
                     /gene="recG"
                     /locus_tag="PXB44_001694"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL5282154.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(5971..6399)
                     /locus_tag="PXB44_001695"
     CDS             complement(5971..6399)
                     /locus_tag="PXB44_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5282155.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(6399..6686)
                     /locus_tag="PXB44_001696"
     CDS             complement(6399..6686)
                     /locus_tag="PXB44_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5282156.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            6850..8733
                     /gene="htpG"
                     /locus_tag="PXB44_001697"
     CDS             6850..8733
                     /gene="htpG"
                     /locus_tag="PXB44_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL5282157.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT         2441 a         1856 c         2039 g         2436 t
ORIGIN      
        1 gagggggcat tatggctatt tatgcaatat ggaataataa aggtggggtt ggtaaaagtt
       61 atttaacttt tcaaatcgct tgtgagtatg caaaacaata cccagcaaaa aaagttctcg
      121 ttgttgattt atgtcctcaa gcaaattcat catctatgct attaggcggc attgaaaaag
      181 gtgagcaaga gttagcaaaa attcattctc aagtaaacaa gaatactatt tcaggatata
      241 tttcagaaag attagtaagt ccttaccgtt ctattaatag tgcaacgaaa tatgtaactc
      301 aagttagcac tataaatgaa tatgtaccaa aaaatgttta tcttgttgtt ggtgatgagg
      361 aattagaaat ccaatcatct ggtattttac atgcttccca agctgttcat gttcctgatt
      421 cttggcggct agtgcatctt tgggtgcgtg atttagtttc tgatatacag aacgcttggg
      481 ataatgatga taacgttgta tttattgatt gcaatccaag tttcactatt tatactgaat
      541 tagcgttggc tgcttcagat agattgatca tccctttttc cgctgatggt tcatcaaaac
      601 gagctgttaa gtctgtatta tctctagtat atggggttaa aaggcactct ggggatgtca
      661 gctctcattt ttatacagaa tctcaaaaga gtaagttagc attacctcaa atttatatgt
      721 atgtaggtaa tagattaacg ataaatgcca aagcatcagc aaccgcattt aaaacagttg
      781 tagaggaaat aggtgatgaa atctataata tatggaaaac taatgctaat ttgttctgta
      841 ttcacccagc aggtgttggt actcctacaa ataaatcaag cttcagaaaa atgtttcagt
      901 atgaggtgag agatgcaaat acagcatctg ttgtttcagg tgctttaggt attccaatta
      961 gtcgcttatc atcaggaaaa agagatgttt taggtaaaaa tgtaatggtg aatcagtcac
     1021 aattagatgc tcaagttcct aacattcaaa atcttgttca gtgtatagaa taaagaaaaa
     1081 tagcgtatat taagtacgct attttttatt tctgacaaat ctttttattt gatagctcga
     1141 tcacaaaaaa agaaatatct ccttttaaat aaaaggactt tgggctaatc taaacagagt
     1201 ttttaaatta cataggagta aaaaatgact attttccaag ttgcacaaaa ttggttagcc
     1261 caagacccgg atgcagagac ccgtgcggaa ttagagcagt taattcaagc ggcagaatcg
     1321 gacgagaaag caaaagcaga attaacagcc cgttttgacg gacgtttaca gtttggtacg
     1381 gctggtttgc gtggtcgctt acaagcgggt tcaatgggta tgaaccgtgt tttggtggca
     1441 caagctgcca gcggtttagc tgggtttatt aaaggttatg ataaagagcc gtcaattgtt
     1501 attggttatg acggtcgtaa aaattccgat gtgtttgccc gtgacaccgc tgaaattatg
     1561 gcagcggcag gcattaaaac gtacctgctt cctcgcaaat tgccaacacc agtgcttgcc
     1621 tatgcgattc aatattttga taccacagcc ggtgtgatgg taactgccag ccacaaccca
     1681 ccggaagata acggctataa agtttattta ggtaaagcca acggtggcgg acaaattgtt
     1741 tcgccggcag ataaagaaat tgctgctctg attgataaag tagcagcagg cgatattcgt
     1801 gatttacctc gtagccaaga tttcacggtg ttagacgatg aagtggtaaa tgcttatatt
     1861 gagaaaaccg cctcactagc caaacggcca aaagccgaaa taaactacgt ttacactgca
     1921 atgcacggtg tgggctatga agtgttaagc aaaacattag aaaaagccgg cttaccgcaa
     1981 ccgtatttag ttagtgagca gattcagccg gacggttcgt tcccaacggt taatttcccg
     2041 aacccggaag agaaaggggc gttagattta gcgattaaat tggcgaaaga gaaaaatgcc
     2101 gaatttatta tcgccaacga cccggatgct gaccgtttag cggtagccgt gcctgatgct
     2161 caaggcaact ggaaaccact gcatggcaat gtgatcggct gtttcttagg ctggtattta
     2221 gccaaacaat tccacgcaca aggtaaacaa ggcgtgttgg cttgctcgtt agtatcctct
     2281 ccggcgttag ctgaaattgc gaaaaaatat ggtttaagct cggaagaaac cttaaccggc
     2341 tttaaatata tcggaaaagt tgaaaaccta ttatttggct ttgaagaagc cttaggttat
     2401 ttggttgacc cagacaaagt gcgtgataaa gacggcattt ccgctgccat tatgttctta
     2461 gatttagtgt gcagcctcaa acaagaaggt aaaacccttg cggattacac caccgagttt
     2521 gtacaagagt tcggggctta tgtaagcggt caaatttcga tcagagtttg tgatttagcc
     2581 gaaatcggca aattaatgac ggcgttacgc aacaatcctc caagcgaaat tggtggcttt
     2641 aaagtggctg agtttattga ccataccaaa accacacgcc aaaatgacat tttagtgttt
     2701 gtgttggaaa atggcagccg tttgattgct cgcccttcag gtactgagcc gaaaattaaa
     2761 ttctatttag atgctcgtgg cacagacgct gaaaacgcag agcaagtgct ggctcagttt
     2821 gacgaaagtg tgcgttcgct gcttcgccaa gaagaatacg gcaaacaaga ttgctaatgg
     2881 cattaattga gggggagctt aggctctccc ttttttaaag gagacaataa tggctcaaaa
     2941 aattatttta gattgtgatc cggggcatga cgatgcgatt gcgatgatgc tcgcttgggg
     3001 gaatccgaat attgatttgt tggcagtgac tacggtggta ggtaatcaaa ccttagaaaa
     3061 agtatcgcgt aatgcgttgg cggttgcgga aattgctcat atttgcaacg ttccttttgc
     3121 caaaggttgt gccagaccgc tggtgcgtga agtggaaaat gccccggata ttcacggtga
     3181 ttcgggaatg gatgggcctg tactccctga atctacccga caatttgaga aaaaacacgc
     3241 cgctcaatta attattgatt tgataatgtc gcacccggag aaaagcatta ccttagtgcc
     3301 gaccggtggg caataaccaa tattgctcta gctgcccgcc ttgagccccg tattattgaa
     3361 agagtgaaag aagtggtgct gatgggcgga ggttatcaca ccggcaactg gagtgcggtg
     3421 gcagaattta atattaaaat tgacccggaa gctgctcata ttgtgtttaa tgccggttgg
     3481 aaggtgacga tggtcggctt agacttaact caccaagcac ttgcgacccc cgatgtggtt
     3541 gaacgcttta aggcattaaa cagcaaatcg gggcagtttg tggtggaatt gctggcgttc
     3601 ttcgccaaaa tgtataaaca ggctcaaggc tttgatgctc caccggttca cgatgcctgt
     3661 gcggtggcgt atgtgattga cccaagcctg attgaagttc agcaagtgcc ggtcaatatt
     3721 gagctaacag gcacgcatac actgggtatg acggtggcgg atttccgcta cccgccaaaa
     3781 cagtgtaata cttgggtagc aaaaaaatta gacaaatccc gcttttggga tttggtaatt
     3841 gaatcggtga aaaacctgcc gtaatctaaa taacccgctt gccggttaag cgttggtata
     3901 ttccgccttg ttgtctaacc aacgcttaat taacgcctct gcaagcggtt gattttcgct
     3961 aataatttgc ttggcgtaac gttgcaccag cggaatcatt ttgcggtctc gcattaaatc
     4021 cgccacctta aattctgcca ttccggtctg ttttgtgccg agaatttcgc ctgtgcctcg
     4081 aatctccaaa tctttttcgg caatgtagaa accgtcttgg ctatcacgca tcacttgtaa
     4141 ccgcttgctg gaaattttcc cgagcggcgg tttgtacatt aagacgcaat gcgaggcggt
     4201 tgcccctcgc ccaacacgtc cacgcaactg gtggagttga gccagcccga gtcgttcgga
     4261 attttcgata atcattaggc tagcattcgg cacgtccact cccacttcaa ttacggtggt
     4321 cgcaaccagc aaatctaaat tcgctgcctt aaattccgcc ataatcgctt gtttttcctg
     4381 tggtttcagc cgtccgtgta ctaacccgat gcgtaaatcc ggcaaggctc gctgtaaatc
     4441 ttcggcaatc gctgccgcgg cttgagcctc caacacttcg ctttcatcaa tcagggtgca
     4501 gacccaatag gcttggcggt gttcttgctt acagacttgg tacacccgtt ggacgatttc
     4561 tgctcgccga tcttcagaaa tcaccacagt cgtgattggc gttctacccg gtggcagttc
     4621 gtcaatgatt gaggtgtcga ggtcagcata gaccgtcatt gccagcgttc tcggaatggg
     4681 ggtcgccgtc ataatgagct gatgcggata gttattttct ttcgctcctt tttcccgcaa
     4741 ggtcagccgt tggtgtacgc caaaacggtg ttgttcatca ataatcacca acgctaaatg
     4801 gtgaaattcc acattgtctt ggaacagggc gtgagtgccg ataatcatct gcacatcacc
     4861 gtttttgatt gcctcaagtt gtgcggttct cgccttgcct ttcactttac cggcaagcca
     4921 gccaacctca atcccaaagg gattaagcca gttggcaaaa ttattggcgt gctgttcggc
     4981 aagaatttcg gtcggtgcca ttaatgccac ctgcttgccg ttatcaatcg ctaatagtgc
     5041 tgccaaggcg gctactaagg ttttacctga accaacatcg ccttgtacca gtcgcatcat
     5101 cgggtgtggt ttggcaagat cttgctcaat atctgcggtt actcggcttt gtgcgtgagt
     5161 aggcttaaaa ggcaaactgt cgagaaaacg ctgttttata tcggtttggt aagtaagtgc
     5221 ctctgcatat tgctgtttta cccctaaacg gagttgctgc atcgctaaat tgtgtgccag
     5281 cagttcttca aaaatcagcc gtttttgagc agggtggtca cctttttcca agacttcggc
     5341 agaaatcgtc ggcggtgggc ggtgcaataa ctgcaacgct tcttttaggc tgtatttgtg
     5401 cggattgtat tcatcaggca acagctcggc gactttcact ttctctaata aagcgagggc
     5461 ttgctcggtt aatttccgca gcgaggcttg ttttaacccc tcagtggtgg agtaaatcgg
     5521 ggtcagggtt tcagccagta caagcggttg attatcccga ataatttgat attcagggtg
     5581 gtgaatttcc gccataaatc gcccacgctt aatttcccca aaggctttta cccgaacgcc
     5641 ggtggcaagg ctatttttca ttccagcatt gaaattgaag aatttaagca taattttgct
     5701 ggtgctgtcg gagagtgtaa ccgataaaat cgggcgttta ccaaactgga cttcggtaag
     5761 ttgtacatag ccttcaatgg tggcaaagct ttccgggcga acatcagcaa tcggggtaat
     5821 acgggtgcga tcttcatacc gcattggcag atgaaacagt aaatcctgca cattattaat
     5881 gccgattcga cttaactttt cggaaattgc cgctcctacg ccggaaaggg cggttaatgg
     5941 cacgccgtct aataattgtt cactcattat ttatgcggtt tcaaatgggt taaataaacg
     6001 cacatcacaa cggataaaat ctttggtgtt gcgggtgact aaaatcagat tgtggtattt
     6061 ggcggttgcc gcaatataag catcatttaa atccatttta ttgggaatat ggtactctga
     6121 tgccaataga gcaatgtcgt tattcaccac aaacgctttt gtttgataag cctttaatac
     6181 attattttca aaccaatcat ttaatcggtc tgattgttct ttgtctttgc gggctttcag
     6241 caaaatacct gttttgattt cagataacgt aatacagctg atattggtct ggctaggatt
     6301 aacccctctc agccactggc gaacattgac atccatcttg tcatttctta acttataaag
     6361 ctcggaaact atattggtat caaaaaggta gcgatacatt actcatctcc aaaatcaaca
     6421 ggtcttggtt tattgctttt gcggatttca accaatgttt gttcaaactc ttgccaaaat
     6481 tcatcgctca catcctcatt ttcggaagag gcgaatagct cttcaaagct tggctcgcta
     6541 ggctttttct gctgataatc catataacta gacaccacat tgacgatttt gccccatttg
     6601 gtaataaaaa ccggctcttt ttctgccatt tttaacactt tgctggcatt ttgattaaat
     6661 tctctggaag taatggttat tgccatatcg ctctccaaaa tgtagtaaaa agatgagtgt
     6721 attttattaa atgtagtaat atctttcaat taattcgcta tttggcaaca gatattttgc
     6781 aaaaaatctt gaaaatctga ccacttgccc catctattcc ctgtttttta atgaataaag
     6841 gaatacacaa tggcaaataa tcaagaaacc cgtggcttcc agtcggaagt gaaacagtta
     6901 ttgcaactga tgatccactc gctttactcc aacaaagaaa ttttcctgcg tgagcttatt
     6961 tccaacgcct ctgacgccgc cgataaattg cggtttaaag ccctttccga gccggctttg
     7021 tatgaaggtg atggcgaact gcgtgtgcgt gtctctgttg atgaaacact cggtacgatt
     7081 acgattgccg ataacggtat cggtatgacc cgtgagcagg tgatcgacca tctcggtacg
     7141 attgcaaaat ctggtaccaa agaatttttg aacgcactcg gcactgatca agccaaagac
     7201 agccagctta tcgggcagtt cggcgtgggc ttttactctt ctttcatcgt ggcggataaa
     7261 gtgacagtca aaacccgtgc cgccggagtg cctgccgagc aaggcgtgat ttgggaatct
     7321 gccggtgagg gcgattacac cgttgccgat ctggagaaaa aagagcgtgg cacggaaatc
     7381 attcttcact tgcgtgagga cgaaaaagag tttttaaacg aatggcgttt gcgtgagatt
     7441 atcggcaaat actccgacca tatcggcctg gcggtggaaa ttcagaccaa tgaatacgat
     7501 gacgagggca aagcaagcgg tcaaaaatgg gaaaaaatta acaaagctca agcgctttgg
     7561 acacgctcaa aaaatgagat tagtgacgag gagtacaccg aattttacaa acacctcagc
     7621 catgattttg ccgacccgct gatttggtcg cacaataaag tcgaaggtaa gcaagaatac
     7681 accagcttgc tgtatgtgcc gagcaaagcc ccgtgggatc tgttccaacg cgagcaaaaa
     7741 cacggcttga aactctacgt tcagcgtgta tttattatgg acgatgcgga agtctttatg
     7801 ccgaactact tgcgttttat gcgtggcttg ctcgacacca acgacctgcc gctcaacgtc
     7861 agccgtgaaa ttctgcaaga gaataaagtt acccaatcgc tccgttctgc cttaaccaag
     7921 cgtgcgttac aactgctgga aaaattggcg aaagacgatg ccgaaaaata tgcccaattc
     7981 tggcaagcgt tcggcgtggt gcttaaagag ggcgtgggcg aagactttgc caacaaacaa
     8041 caggttgcag gcttactccg ttttgcctcc acccagcgtg atgacagcga acaagcggtc
     8101 agttttgccg attatattgc aagaatgaaa gaggggcaga aagcgatcta cttcttaacc
     8161 gcagacagct accaagcggc taaaaacagc ccgcacttag agctgttcca caaaaaaggc
     8221 attgaggtgc tactgctttc tgaccgcatt gatgaatggt tggttagcca cctcaccgag
     8281 tttgacggca aaccgctaca aagcattacc aagtcggact tagacttagg cgagcttgcc
     8341 gacaagcagg aagaagagag ccaaaaagcc caacaggcgg agtttggttc gttcttagaa
     8401 cgggcgaaag gctactttgg tgaacgggtc aaaaacgtgg tgctgactca ccgcttaacc
     8461 gacacaccgg cagtggtttc cactgacagc gatgaaatga ccacccaaat ggcaaaactg
     8521 tttgcagcaa tggggcaaca agcaccagaa gtgaaatata cttttgagct gaacccagat
     8581 cacccaatgg tgaaaaaagt agctgatctt gccgatgaag ccgagttcgc cgattggatt
     8641 gaactgctgt ttgaacaagc tctgcttgct gaacgaggca cgttggaaaa cccgacagcc
     8701 tttattaaac gggtaaataa attgttgggc tagccattac taaaaacgga aatctaggtt
     8761 tccgtttttc ca
//
LOCUS       DANLUA010000102         8768 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLUA010000102 DANLUA010000000
VERSION     DANLUA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            2..1300
                     /gene="pilQ"
                     /locus_tag="PXB44_001698"
     CDS             2..1300
                     /gene="pilQ"
                     /locus_tag="PXB44_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL5282158.1"
                     /translation="MYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDN
                     IETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPIMNLQN
                     NGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSNSLIVK
                     DSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGG
                     RLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPR
                     LLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQILLDLIV
                     TQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIP
                     FIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
     gene            1381..1794
                     /gene="nusB"
                     /locus_tag="PXB44_001699"
     CDS             1381..1794
                     /gene="nusB"
                     /locus_tag="PXB44_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL5282159.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            1857..2837
                     /gene="thiL"
                     /locus_tag="PXB44_001700"
     CDS             1857..2837
                     /gene="thiL"
                     /locus_tag="PXB44_001700"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL5282160.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            2842..3315
                     /locus_tag="PXB44_001701"
     CDS             2842..3315
                     /locus_tag="PXB44_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL5282161.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            3322..3945
                     /locus_tag="PXB44_001702"
     CDS             3322..3945
                     /locus_tag="PXB44_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL5282162.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            4057..4662
                     /gene="ruvA"
                     /locus_tag="PXB44_001703"
     CDS             4057..4662
                     /gene="ruvA"
                     /locus_tag="PXB44_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL5282163.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            4799..5803
                     /gene="ruvB"
                     /locus_tag="PXB44_001704"
     CDS             4799..5803
                     /gene="ruvB"
                     /locus_tag="PXB44_001704"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL5282164.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(5902..6585)
                     /gene="artM"
                     /locus_tag="PXB44_001705"
     CDS             complement(5902..6585)
                     /gene="artM"
                     /locus_tag="PXB44_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL5282165.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(6585..7256)
                     /gene="artQ"
                     /locus_tag="PXB44_001706"
     CDS             complement(6585..7256)
                     /gene="artQ"
                     /locus_tag="PXB44_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL5282166.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(7256..7981)
                     /locus_tag="PXB44_001707"
     CDS             complement(7256..7981)
                     /locus_tag="PXB44_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL5282167.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(8007..8741)
                     /gene="artP"
                     /locus_tag="PXB44_001708"
     CDS             complement(8007..8741)
                     /gene="artP"
                     /locus_tag="PXB44_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL5282168.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         2637 a         1873 c         1695 g         2563 t
ORIGIN      
        1 tatatatttc ttatgcggtt tttcattagc ctatgcgaat accatttcca tagttttaaa
       61 aaatgctcca acttcgctta tttttactta tttagcggaa gaaaccggta aaaatatggt
      121 gcttgatgat aatattgaaa ccaaatcaac actacgatta gaaaataaat cggttgacga
      181 gatttttaaa acgcttggca aagtcaataa attatcctta acgcaagaag atgatattgt
      241 ttatattcac aaaaaagagg aaaaagcggc tgacttaacg cctctgccaa taatgaattt
      301 gcaaaataat gggcaaaata caccgcttgt caccgcccct aagttaatca caaaaacctt
      361 gaaactacat tatgcaaagg cttcagaggt gattgagtcg ttaaccaaag gcagcggaac
      421 gtttttatcg gaaaacggtt atattcactt tgatgaaaga agcaatagcc tgattgtgaa
      481 agacagtgca aaatcgctta aaaatatcga gaatttagta aaacagctcg accaacccac
      541 cgagcaaatt gcgattgagg ctagaattgt cactatcagt agcgagcatt tgcaagagct
      601 tggcgttcgc tggggaattt tttctcgtgg ggctgggcac tataaatttg gcggacggct
      661 ggaaggcaat ggactcaaca atgtcaccaa taacttaaat gtaaattttc cgattactgg
      721 tggagcatct gccgtattac aggtttcgtc cattaatagc agagtgcttg atttagaatt
      781 aagtgcatta gaacaagaaa atagtgtgga aattattgcc agcccacgcc tgttaaccac
      841 caataaaaag ccggcaagta tcaagcaagg cacggaaatt ccttatgtga tgtacaacac
      901 caaatcggaa gccaccgatg tcgaatttaa agaagcggta ttagggcttg aagtcgttcc
      961 acacctttca acccaaaatc aaattctact cgatctgatc gttacccaaa attcgccaaa
     1021 ttcccaatcc ggcagtagtg gcttaattac gattgataaa caagagctaa atacgcaagt
     1081 atttgccaaa cacggcgaaa ccattgtact tggtggtatt tttcaacatt taacccaaaa
     1141 aggcgaagat aaagtgccga ttttaggctc aattcccttc atcaagcggt tatttagtca
     1201 aactcgagat aaaatcagca aaagagagct agtcattttt gtgacgcctt atatccttca
     1261 atctaatgaa aagagcagca accgcaaaaa atcaaaatag cacatcataa acgcaagcaa
     1321 aaatagggga agtgtgatag aatcagccgt cattttttga gttaaagatt tagaggtttt
     1381 atgaaaattt ccccacgtcg ccgtgcaaga gaatgtgcgg tgcaagccat ttattcttgg
     1441 tatatttccc aaaacacggt tgaagaagtg gaattggcgt ttgtgaccga tcaagatatg
     1501 aaaggcgttg atatgcctta tttccgtaaa cttttccgtg gcgttgtgga taatgtagaa
     1561 gccattgatg aagcattacg cccttattta gatcgtaaag agtcagatgt tgatcctatt
     1621 gaacgttcta ttttgcgttt atcaacttac gaattaaaat ttgaacacga tgtgcctttc
     1681 cgtgtagtca tcaatgaagg gattgaagtc gctaaagtgt tcggctctga tgacagccac
     1741 aaatacatta acggcatttt agataaatta gccccagcat taggcagaaa ataataagat
     1801 tccccaattt atacataaat tggggatttt gattataacg ctcaaggaga ttttttatgg
     1861 gtgaatttga tattatcgaa cgctatttta atgcttctca acgcccacct cgcaaagatg
     1921 ttttactttc tatcggcgat gactgtgcgc tcacatcact caaaccaaat cagcaacttg
     1981 cgatcacaac cgataccctt gtttgcggca ctcattttct accgactatt tcacctgccg
     2041 atctcgctta taaaagtgtg gcgtccaatt taagcgatct tgccgcaatg ggtgcagaac
     2101 ctgcatggat ctctttagcc ttaaccttac ctgaaattaa tcacgattgg ctagctgaat
     2161 ttagtgaaca tttctttgag attcttgatc actataatgt tgatttgatt ggaggggata
     2221 ctacttgtgg accactatct ttgacaatca cagctcaagg gattgtgcca caagatagag
     2281 gcttattccg ccataatgct aaagtaggcg attggatcta tgtttccggc acactcggtg
     2341 acagtgcagg tggtttgcaa cttctgctaa atcagcaaag cgagcaagtg aattggaata
     2401 aagagcaaag ctacctgatt caacgccatt tacgcccaac acctcgagtt ttacttggct
     2461 tgactatcgc ctctattgct aatgcagcga ttgatatttc agatggtcta ttgtctgatt
     2521 taagccatat tttaaagcgc agtgactgtg gtgcagtatt aaaccttgag aatttcccct
     2581 tgtctgacgt cttacttagt tgttattctc gaacagaggc agaatatttt gccttaaacg
     2641 gtggcgaaga ttacgaactc tgctttaccg tgccaaatga caacaaagcc aaattagaca
     2701 aagcattagc caatatcgga gtaacttata catgtattgg gcaaattcgt tcggctaaag
     2761 aaggtttaac cctattacgt cataatcagc ctgtcacctt gccctcacaa cgaggctacg
     2821 atcattttaa aaaataacgc aatgaaacca attaatctta aaaatccgat tcattttttt
     2881 gcctttggct ttggttcagg cttattaaag cctgctcccg gcacctgggg tagcctcgcc
     2941 gggctaattg tagcaatagc cttgtggtct atcacacaat ccgccctgtt tttcggctta
     3001 ctcaccctag tttcttttat tgccggttgt gttatttgca ccaaagccag ccaagatcta
     3061 gaagtacacg atgacgggcg aattgtttgg gacgaaatag ttgccatctt tttgatgttc
     3121 acctgtttgc ctgaatacaa tcttcttggc tacctgctcg cctttatcag ctttcgcatt
     3181 tttgatattt tgaagccttt tccaatccgc tattttgatg aaaaattaga aagcggttta
     3241 ggcattatgt ttgatgatat tctcgcagcc atttttgccc tgatttcact tcatctttta
     3301 tattatttca tttaaaaatt tatgctcact attttattta ttcattttgc cgggcttatc
     3361 agccccggcc ccgatttctt ctatgtcgtc agacaatccg ccagccactc agttaaaagt
     3421 ggggtacttg ccgcaaccgg aatctcaatc ggcattattt tttgggcagc ctttgcgatt
     3481 tttggtttag cgtggctcag taatagtatt ggctcgctgt ttcaatatgc cattatgatt
     3541 atcggtggct gttacctggt ttatattggc tctcaaatgg tacgggtaac cgaaaatatc
     3601 atttttgttg aaaataaagc tcaactcacg ccgctaaacc cttggcagga aattaaaaaa
     3661 ggcttattaa tcaatatctt taatgccaaa gctggcgttt actttaccag cgtcatttca
     3721 gcctatgtag ataatttaag caatacccta caaatgttcg aactacttct actctttgtg
     3781 gtaagtacct ttatttattt ctgtgtggtg gcactactct tttctcgccg accggtcaga
     3841 ctgttctatg ccaaatatag tcgctatatt gacaatatct caggcgtaat tttcatctta
     3901 ttcggctcga tgctgattta tgaaggattc agccacttta tctaatcaca agcggtctga
     3961 tttcactaaa aatttgcaaa ttccgctatc aataaatagt gttattttat acagtttctg
     4021 ctatgctact gccaatttta actctgagag aacaaaatga ttggacgatt acacggcaaa
     4081 attattgaaa agcaaccgcc tgaaatttta ttagacgtgc aaggggtagg ctatgaacta
     4141 ctgctcccga tgaccagttt ctacagcctg ccacaaattg gcgaagaggc gactattttc
     4201 acccatttag tggtgcgaga agatgctcac ttgctattcg gctttgccca aaaacaagat
     4261 agaactctat tccgtgaatt aattaaaacc aacggtgtcg ggccgaagct cgcgttggca
     4321 attctatcag caatgtcggt gtcgcaattt gccaccgcga ttgaaaatga agagctggca
     4381 aaattaacca aaattccggg aatcggtcgg aaaaccgcag aacgcttatt ggtggaactc
     4441 aaaggcaaat ttaaaggaat ggcacagagt gatttctttg tggaagtatc gcacgagcaa
     4501 attgtggcaa gtaatacgcc agacccggca aatgaagccc ttgatgcctt aatagcccta
     4561 ggctacaaac ctgccgatgc ggaaaaaatg attaagaaag tgaataaatc cggtgcaacg
     4621 agcgaacagc ttattcgtga ggcgttaaaa aattcattat gatattgata gtatagggcg
     4681 aaagatattt cgcccctaca atctcatttt taaaaatata tgtgtagggg caaatcaata
     4741 tttgccctca tgagtaatat agggtaatac cattccacta caacaataat ctaaatgtat
     4801 gattgaagca gatagaatca tcagtgcctc gcctaagcgt gaggaagaag ttatagaccg
     4861 agcaatccgc ccgaaactgc ttgccgacta tgtcgggcag ccttctgtgc atgatcaaat
     4921 ggagatcttc atcaaagccg ctaaattgcg ggaagaggca ttagatcact tgctcatttt
     4981 cggcccgccc ggtttgggaa aaactaccct tgccaacatt gtggcaaacg aaatgggggt
     5041 gaatattcgc acaacttcag ggccggtgct ggaaaaagca ggcgatttgg cagcaatgct
     5101 gaccaacctt gaaccttacg atgtgctgtt tattgatgag attcaccgcc tttctccagc
     5161 gattgaagaa gtgctttatc ctgcaatgga agattaccag ctcgatatta tgattgggga
     5221 aggacctgct gctcgttcca tcaagctcga tctaccgcct tttacccttg taggggcaac
     5281 cacccgtgcc ggctcactga cctcaccgtt acgagatcgt tttgggattg tacaacgctt
     5341 agaattttac tcagtggacg atcttactct gattgtcaaa cgcagcgccg attgcttaaa
     5401 tctcaatttg tcggcagacg gtgcttatga agtggctcgc cgctcccgtg gcacaccccg
     5461 tattgccaac cgcttactcc gccgagttcg ggattacgcc gatgtgcgta ataatggcat
     5521 tattacctca gacattgcca aacaagctct ggcaatgctc gatgtggatt cggaaggctt
     5581 tgattttatg gacattaaac tgttgcaagc gattgtagaa cgctttgatg gcgggccagt
     5641 tggattagac aatcttgcgg cagctatcgg cgaagaacga gatacgattg aagatgtgct
     5701 agagccttat ctgattcagc aaggctttct acaacgcaca ccaagaggca gaattgctac
     5761 cagcagaacc tatgcccatt taggtataac ccaaatagat tagtcatatt tctaaaggca
     5821 ttgatacata aataaaaagc ggatagttag tactatccgc tttcatttgc aaaaaatcaa
     5881 taaaatttga ccgcttatcc gctaggcttt ttcagtctca aaacgctcaa aagctaatac
     5941 tctgctttct aattttctga gaacgattgt cataatgccg gtaattacca agtaaataac
     6001 ccctgccata ccataaattg ataatgcgtc atactctgta ccataaagct ggcgagcata
     6061 gcccataata tccataatcg taatggttga agcaagcgat gtgcctttaa aaactagcac
     6121 aatctcatta ctataagaag gtaaagcacg ttttaatgcg taaggcacta aaattttcag
     6181 tgtatctaaa cggctcaaac caagggctgc gcaagtttcc cattgcccct tcggaatcgc
     6241 ttttaccgca ccgtggaaca acaatgtcga ataggcagca ctgtttaacg ctaaagccaa
     6301 cattgcacag aaccaagcgt cggataataa tacccaagcc gcactttctg taatccattc
     6361 aaattgcccc gggccactat aaatcaagaa aaattgaatt aataaaggcg ttccggtaaa
     6421 aagcgtaata aatccgttta ccaaccactt taacggacga ttttctaacg agagaataaa
     6481 cgtaagaatg atcgctaaaa taaaggcaat gatgagcgcc acaaaagtga gctgtaggct
     6541 ggtgccaata ccctgtgaaa tttcaactaa ataatcccac cacattaagc aactcctcgc
     6601 tcaaaacggg taaatctacc ttctaatact ttaatcactc gctggctaat tagagtaatc
     6661 gctaaataaa taagtgctgc tatcccatac caagtaaaag gttgatgagt attagcatta
     6721 attaactccg attgacgcat taaatcatct accccaatta acgataccaa tgccgtatct
     6781 tttaacagca ctaaccactg attgcttaag cctggtaaag cgtgtcgcca gacttgaggg
     6841 agaataatgc gaacaaatac ttgcgctcgg cttaacccta acgccgtacc agattcccac
     6901 tgcccaactg gaatagcttt aattgcacca cgtaaagatt gggaagcata agcagcaaaa
     6961 ataataccca gcgcaatagc cccacaccag aaagcattta actccgcaaa ctcatcggtg
     7021 atcaagaaaa taacttggat aagaccaaaa tagatcaaaa ataccactaa aatctccggc
     7081 aacccacgca ataatgtaat tattatacta gttggtttac ggatacatgc aatcttactg
     7141 gtttcaagta ccacaaaaaa gattgacaat aacattccaa tcaagaggct tgccagcgca
     7201 atccctaagg tcatcagggt tgcgttgaca agtaagttta aaatatcgct agacattatt
     7261 tcccagccat ccaagtatta taaatttgct catatttacc atttgttttg atggtagcta
     7321 aagccgaatt taatttttca agcaatgcag tgttagattt atttaccgca ataccatatc
     7381 cattaccaaa aaaggcttta tcgactactt tttcacccac gaaatctaac tctttttcgg
     7441 tttttagcca ctctgccaat actggtgtat caccaaatac catatcaaca cgtccatttt
     7501 ttaaatctaa cactgctgat tgtaggctag cgtaaggttt taaattatat tgtttggcat
     7561 ttttgcttaa gtattgttga aaagttgtac cattttgcac gccgacattc ttagctgttt
     7621 ccggtgtaaa tttccccttc attgcgacat aacttactga gttttctaaa taagcattgg
     7681 taaaggcaac ctgtttttga cgcgcttcag taatatccat gcctgaaata attgcatcaa
     7741 aatttttaaa ttttaatccc gcaattaagc tatcgaatga ttgcgtttta aattcgcaag
     7801 ttgcttttag ttcttcacat aatgcttttg caatatctac atcaaagcca ataacttcac
     7861 ctttttcatt tgttgtttca aaaggggcgt aagaaggctc catcgcaaaa gtgatagttt
     7921 cttgtgcatt ggcagaaagc gttgcaaatg cgatagcgga agctaataat aattttttca
     7981 ttgttgtgtc cttttgttgt tatagattac tctgagtgag ataaatattg agcaaattgc
     8041 tctgttttcg gtgattcaaa acaacttgca tcaccttgtt caataattgt acctttttcc
     8101 atgtacacca ctttgcttgc caccttacga gcaacgccta cttcgtgagt cacaatcacc
     8161 tgagtaatgc ctgtagattg caattcttta ataatatcaa ctacttgtgc agtaatttcc
     8221 gggtcaagtg ctgcggtagg ttcatcaaat aataagacct gtggctgcat catcagtgct
     8281 ctggcaatcg ccacccgttg ttgctgacca cctgaaagat gcagtgggaa acgctcggca
     8341 aactcgctca aacgcaaacg tgccaaatgt ccttttgctc tggcaaccgc ttcttctttg
     8401 cttaacccaa gcactttcat cggggcttca attaagtttt gcattacggt taaatgcggc
     8461 cataaatgat actgctggaa caccattccc acctcacggc gtaacaaact caccgctttg
     8521 ctgtcggttt tagcagttaa atcaaattga tgatttgcaa tcgccaaagt gcctgattgc
     8581 ggtacttcca ttaagtttaa ggttcgaatt agggtacttt tacctgcccc actcgggcca
     8641 agcaatacaa cagtatcccc tttttcaatc tctaagttaa tgtcaaataa ggcttggttt
     8701 gagccataaa aaaaattgac attttgaata cgaattgcca ttcgttaaaa tcctactaca
     8761 tttaaaat
//
LOCUS       DANLUA010000103         8724 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLUA010000103 DANLUA010000000
VERSION     DANLUA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            33..302
                     /locus_tag="PXB44_001709"
     CDS             33..302
                     /locus_tag="PXB44_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5282169.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            315..1665
                     /locus_tag="PXB44_001710"
                     /pseudo
     CDS             315..1665
                     /locus_tag="PXB44_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1760..2908)
                     /gene="dprA"
                     /locus_tag="PXB44_001711"
     CDS             complement(1760..2908)
                     /gene="dprA"
                     /locus_tag="PXB44_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL5282170.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            2997..3509
                     /gene="def"
                     /locus_tag="PXB44_001712"
     CDS             2997..3509
                     /gene="def"
                     /locus_tag="PXB44_001712"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL5282171.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3603..4529
                     /gene="rfaD"
                     /locus_tag="PXB44_001713"
     CDS             3603..4529
                     /gene="rfaD"
                     /locus_tag="PXB44_001713"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL5282172.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4597..5127)
                     /gene="fabA"
                     /locus_tag="PXB44_001714"
     CDS             complement(4597..5127)
                     /gene="fabA"
                     /locus_tag="PXB44_001714"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL5282173.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5240..6877)
                     /locus_tag="PXB44_001715"
     CDS             complement(5240..6877)
                     /locus_tag="PXB44_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5282174.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSELFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            6970..7416
                     /gene="matP"
                     /locus_tag="PXB44_001716"
     CDS             6970..7416
                     /gene="matP"
                     /locus_tag="PXB44_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL5282175.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(7482..8618)
                     /gene="ompA"
                     /locus_tag="PXB44_001717"
     CDS             complement(7482..8618)
                     /gene="ompA"
                     /locus_tag="PXB44_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL5282176.1"
                     /translation="MKKTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQF
                     DNTYDYGSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTT
                     FSGDDKEKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSR
                     IKVHNLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQ
                     YSPDIHSVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDA
                     AHAEISNLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAV
                     GYGEANPVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT         2636 a         1800 c         1657 g         2631 t
ORIGIN      
        1 aactattcat tcacctttaa tggagattta ctatgaaaat tatggcagta tgtggttccg
       61 gcttaggtag cagctttatg atggaaatga acattaaaaa agtattggca aaactcggtg
      121 tcgaagcgga agtgagtcat accgaccttt cctctgttgt tgctgatgat gctgatttat
      181 ttgtgatggc aaaagatatt gccgcaagca gcaatgtgcc gaaagaaaaa ctcatcgtgg
      241 taaaaaacat tgttggatta gcagaattcg aagaaaaatt gaccgcttac tttaaccaat
      301 aacggagatg ttatatggaa tcgctactct tttttatatt agatattctc aaagtgcctt
      361 ccgttctagt gggtttaatt gctctggtcg gtttagttgc acagaaaaaa tcggtatccg
      421 atattgttaa aggaacaatc aaaactattt taggcttctt agtgttaagc ggtggtgcaa
      481 cagtactttt aagctccctg actcctttag gcggtatgtt tgaacacgcc ttcaacgtgc
      541 aaggcattat ccctaataac gaagcgattg tgtctatggc aatcgaaaaa tatggtacgg
      601 cgactgcgtt aattatggca ttcggtatgg tagccaatat tttaattgca cgttttaccc
      661 gcctaaaatt cattttctta accggacacc ataccttcta tatggcgtgt atgatcggcg
      721 taatcctaac cgttgccggc tttgaaggta ttcagctcgt ctttgtcggg gcattaacct
      781 tagggttaat tatgtttccc gactatcgct cacttctata tgaaaaaagt gaccgctagc
      841 aatgatgttg ccttaggtca tttcggcaca ttgggctacg tgcttgctgg ttcaatcggg
      901 caactggtag gaaaaggctc taaatctacc gaagaaatgg atttacctaa aaacctcggc
      961 ttcttacgtg atagctccat ttctatttca ctcacgatga tggcgattta ctatattctc
     1021 gcccttgctt cgggcagtga atacgtttct acattaagcg gcggacaaca ttatttagtg
     1081 tatgccacca ttcaagccat cacttttgca gccggtgttt atgtcatttt gcaaggggtt
     1141 cgtttaattt tagccgagat cgtcccggca tttaccggct tctccgaaaa attagtacct
     1201 gatgccaaac cggctctcga ctgcccgatt gttttccctt acgcccccaa tgcggtatta
     1261 gttggatttc ttgccagttt cttaggtggg attttaagct tagccattct agggcaactt
     1321 aattgggtgt taattttacc gggcgtagtg ccacacttct tctgtggggc aactgccggc
     1381 gtatttggga acgccaccgg cgggcgtcgt ggtgcaatta tcggtgcttt tattcacggc
     1441 atattaatta ccttcttgcc tgtattctta ttacctgtac tgggttcatt aggctttgct
     1501 aacaccactt tctctgactc agacttcggc ggtattggga ttgtgttagg ctatatggct
     1561 caatacttcg acaagaatat cattacgctc attattgtcg gcttattcgc tctgttggta
     1621 gcctataatt atttggcgaa aaaaccggca aaactgaata cctaattaaa atcaggtgtt
     1681 atttgctgcc tgcgaggaat cttccctgcc atttcttgat ggtagggaat tttgtttagt
     1741 taaacgacaa cgctcttgtt caatacccat tatccatcac atacccccca ctcacttgtt
     1801 taatcacacc ggctaattcc agacccagta attcaaccag caatatttca atcgtcaact
     1861 ccgtaacttt ggctaaatca tcaattgcca tcggctctaa gctgatatgc tgataaattt
     1921 gctgctggca agcggtcaga cttggcagat tttttgcgaa tttttcgctg tttttgaccg
     1981 cttgtaaaga tgtgcttact gttggcttag actcatcaaa caaagggatt tgctgaggtt
     2041 gatattgcga ggcgaccacc tctaaaatat cttccacacc ttccacgagc aatgcccctt
     2101 ctttaattag cttgtggcaa ccttgtgcgt aagggttttg taccgcattc ggtaacgcaa
     2161 acacttccct accttgttca agggcataac gagcggtaat cagtgaacca ctattaagcg
     2221 ttgcttcaat aactaaagtg ccgagcgata agccactaat aattcgatta cgacgaggaa
     2281 aattttctgc caccggcggc tgattaggcg agaactccga caccaatgca ccacctaaat
     2341 caacaatttg ttcggctaat tttttatggc gagcaggata gacttgattt aatccactcc
     2401 ctaacacggc aatcgttatg ccttgttcca taacgatttt ttgatgggca aaaccgtcaa
     2461 taccaatggc taaaccgctg gtaatgataa tattatggcg agctaattcg gcagcaaatt
     2521 gcccagccca atattcacca taatgagaat agtctcggct gccaacaatc gcaatttgtg
     2581 gcagagaaag gctttccaca tttcccttta cataaattaa cggtggcgca gtcgaaattt
     2641 gttttaagag aaacggataa ttttcatcaa agagtgtcac aatttgctga tttggcaatt
     2701 ctgaccaagt catcgcctga tcaatccact tttgatcagg attaaaccaa cgttgaattt
     2761 gcttttcatt ccaacctatt tgtttgagtt gaagtttgtc atagtggcaa aattcagcaa
     2821 aattgagttc cgccaataat cgccctatac gttgagcccc catttgcggc acttgtaata
     2881 atcgtaataa atcgtagtga tatgccatat tttcatcctc aaaatttccc taataataag
     2941 aaaaggttta aaatagccaa acgaattttt taaacttttc gagagagatc acaaaaatgg
     3001 caatattaga tgttgtgctt taccccgatg agaaactggc aaccgtttgt gaaccggtgg
     3061 aacaggtgga cgaagcactg aatcgtttta tcgatgatat gtttgagaca atgtacgaac
     3121 acgaaggtat tggcttagct gcaccgcaag tgggcgtact taagcgtgtt attactattg
     3181 atattgaggg cgacaaaacc aatcaagtgg tgttaattaa ccctgaaatt ttagaaagca
     3241 gcggtgaaac cggcattgaa gagggctgtc tctccattcc cgggcatcgt gccttagtgc
     3301 cacgcaaaga gaaagtgaaa gtcaaagccc ttaaccgcca aggcgaagaa attatcatcg
     3361 atgccgatga tttatttgcg atttgtattc aacacgaaat cgaccattta aacggcgtgc
     3421 tttttgttga ccacatatcc aatttaaaac gccaacgcat caaagaaaaa atgcaaaaat
     3481 tgaaaaagca aattgcacga gcgaaataac caggcacaag cggttaaaat ggcttaaaaa
     3541 tttgcaaatt aagtgtgatg acacgataat aaccacagtt caactttaga aaggataaaa
     3601 atatgattat cgtcaccggc ggcgcaggta tgattggcag taacattgtt aaagccttaa
     3661 atgaaatcgg tcgcaaagat attttagtag tagataacct caaaaacggg gaaaaattta
     3721 tcaacttagt ggacttagat attgcggatt attgcgataa agaggatttc atcgcctcta
     3781 ttatcgccgg cgatgatttt ggtgagattg atgcggtttt ccatcaaggg gcttgctcgg
     3841 caaccaccga atgggacggc aaatacttaa tgcaaaataa ctacgaatat tcaaaagagt
     3901 tattgcattt ttgcttagat cgccaaattc ctttcttcta tgcctcaagt gcggcaacct
     3961 atggcggacg ttcggataat tttatcgaga aacgggagtt tgaaggtcca ttaaatgctt
     4021 atggctactc caaattcctg tttgatgaat atgtccgccg tattttgcct gaggcagaat
     4081 caccggtctg tggtttcaaa tatttcaacg tatacgggcc tcgtgagcaa cacaaaggtt
     4141 caatggcaag cgtggcattc cacttaaaca accagatgtt aaaaggggaa aatccaaaac
     4201 tatttgccgg ctcagagcag ttcttgcgtg attttgttta tgtggaagat gtggcaaaag
     4261 tgaatctttg ggcgtggcaa aacagcattt ctggcatttt caacctcggc accggaaaag
     4321 cggaatcttt ccaagaagtt gcgaatgcgg ttatcaaata ccacggcaaa ggcgaaattg
     4381 aaaccatccc attcccggat catttaaaat cccgctacca aacgttcact caagcggact
     4441 taaccaacct aagagccgct ggctacacag gcacatttaa aaccgttgct gaaggaacag
     4501 cggaatatat ggcgtggttg aatcgctaat caaacgttgt taaaaaagca gaaagagcta
     4561 cgaaatgtag ctctttttgt tataaggtta taaggattag aatgctgtcg tatcttggaa
     4621 taagcctact tttaaatcag tagcagtata gatcactcgc ccgtccactt cgacttcacc
     4681 atcagcaacc cccatcacta acttacgatt gaccacacgt ttcatatgga tacggtaagt
     4741 aacttttttc gctgtcggta aaatttgtcc ggtgaatttt acttcaccta cgcccaatgc
     4801 acgccctttg ccttcgccac caatccagcc gagataaaaa ccaaccagtt gccacatcgc
     4861 atctaagcct aagcaacccg gcattacagg atcgttaata aaatggcagt taaaaaacca
     4921 taaatccggt ttaatatcaa actcagcttc aatataccct ttttcaaaat tgcctttttc
     4981 ttggttcatt tcaatgatgc gatccatcat taacatcggt ggagccggca attgaggccc
     5041 tttttcaccg aataattctc cttttcctga tagcaaaagc tcttcgtaag tatagcttgg
     5101 tttgataatt ggtgtacaac tgttcattat ttttaaaatc ctgtaaagtg aaaaaagcct
     5161 gttatatcaa taactgataa atcaggctga tagtaacaga cttttaattc agtgaacagt
     5221 tgtaagctga cttgtcggat cagtctagct ttatcttttt gcaaattttg cttaaaaatg
     5281 aaccgcttgt ttcttcactt tcccgctcat ctagattctg gtgaatcaag gtaaataatg
     5341 ctgtttgggc tgagttaggg gctttctcat catcataaaa atcccgctca aacagaatcg
     5401 ctatggcatc aaacacattt tccaccgtcc aaattttaaa tagaccttgc tccactgcct
     5461 caacaacttc tgaacttaaa ctcaaatggc taacacaggc cgccggaata atcacacctt
     5521 gtttccccgt taattggcga gcgtgacaaa tattaaagaa gccttcaatt ttctgattta
     5581 ccccgcccac gcttagcaca ttcccaaatt gatcgatagc acctgtcacc gcaatagatt
     5641 gtggcaagcc aactttagct aaggcactca ctagtacatt gaaaatcgca agagaagaac
     5701 tatccccatc aatttcacca taggattgtt caaaagcaat cgaagctgaa aacggtaact
     5761 gggttggtag ttctaacaga ttcgctaaac aagattgagc gataataata cctttggagt
     5821 ggatatttcc ccctagctcc actttacgct caatatctgt aatttcgcca tcaccatatt
     5881 gtacattaca actcatgcgt aagggctcgc caaaactatg cggaatgcct tcaaactcaa
     5941 tgaccgataa gccattgatc tgcccaattt cctctccttc ggtttcaata taaagttgat
     6001 tagtcagaat atcacccacc gtatattgat ttaatatcga tgattgctgt tctaaataat
     6061 caaaataacc ttttacctcg tcaatttcgg tcacatttgg gtaaaaattt tgaattccta
     6121 ataaatgttt tctcaataat gtaggggaaa tagacaccaa ctctcggctt tcactttcac
     6181 gtatataggc ctgtaaaaat tgattaagcg ctgtggaagt taatcgctta ttgatgagat
     6241 tctcagcata attctgaaca taacctcccc actgctccac attatccgat aatgataagt
     6301 aggattttat ttcggtatat tgcccaaatt ggtacaaccc atcatcataa gcagataaag
     6361 tagaaatatc ttctctttcc cctactaaaa tcaatttaaa tgttgatttc tctttagccg
     6421 ataaataggg tgagtgatta acagaatttg gctcatattc accaaataat aatgcctgtt
     6481 ttagcttatc ccattgggta atatccaata agagcgaacg gatattaaca attaaaattc
     6541 ccttatctgc ctcgtgaatt gcacctttaa catattctgt tttcagctct ctttccaaaa
     6601 acacagaata accaaataga ctatttttat tgaaatccat tttgctaata atcggcagat
     6661 ctacactata ttttttcaga tattccgtga tttcattgat aaattccgga aaaagctcag
     6721 attttaagac taataaagca ccaaactgag attgtaaaaa ttgatttaac gcttgttttg
     6781 cttgaggttg aaaatctaaa aaattcaatt tctcatctaa acgagagaaa tcattttgca
     6841 ttactaattt ttggctacta agtaaagaaa gggtcacaaa tctgcctcat tattgaaaat
     6901 ttggtttatt atacaaaatt ccgctatact tgctaaatat agcttacttt ttcctatcat
     6961 attcagatta tgcgatatca gaaactcatc acccaagaaa caacttggaa atggcaatat
     7021 ctcctaaaaa aacatcgtga aggagaaaat attactaagt acgaagaaca aagcttgatc
     7081 gatttaaaag ttcagttcct ctctactttg caaaactctc cggacgaagt ggaaaaatgg
     7141 attaagtcgg aaatgacgcc tgagcaacgt aagaaaatgc gtcaatcagt acgcgccaaa
     7201 cgcaaacgct tctttaatgc tgaaaagcaa acaaccaaga aaaaatccat tgatttagag
     7261 tattcgagct ggttaaggct ctctaaatat gccaaaagcc aaaaactaac gctttctgaa
     7321 gctattgtca aattaaccga tgaactcgaa aataaacaac tttacttaga gcaagttgct
     7381 aaaatgaaat caagtttaaa agatttactg aaataaaaaa agaaaaaagc ccactaacaa
     7441 gcgggctttt tttgattcaa tttttcaact gaataactat attacatagt tacttctttt
     7501 gaaccttgaa cttggatttc aacacgacga tctggtgcta agcaagcgat taatgcttta
     7561 cgacctttaa ctgcatcaca tgtgtgacca gttactgggt ttgcttcacc gtaacctaca
     7621 gctgttacat tagctgggtt agtaccttta gaaacgatgt aattagctac tgtttcagca
     7681 cgacgttgtg aaagttttaa gttagatgct tctttaccga tgcggtctgt gtaaccatta
     7741 acttggatag ctaggtttgc taagcctaag ttgctgattt cagcgtgtgc tgcatctaaa
     7801 gaagttgctg ctgctggttt taagcttgat ttaccgaaat caaataaaac atcagagctg
     7861 aatgcgaagt ttttagttac aacttctggt gcttcaactg gtgctgcacc ttgaccgaaa
     7921 cggtatgata aacctgcaga tactgagtgg atatctgggc tgtattggaa atttgtacct
     7981 cttggtatta gataaccaac tgttgtacga actgccttat ctaagttacc aacacgattt
     8041 aagtattgat attcaacacg agcagctaat tcaggagtaa ttgcatactc aagaccagca
     8101 cctaataata gagatggttt taagttgtga actttaatgc gtgaatcttt atctacatgt
     8161 tgttgtacgt agtaatcgtt gcgaactaat gcaacaccta ctttaccgta aacatctaag
     8221 ttaggaacaa cttcgtagct tggttttaag cttaagtgcg caccgtgagc tgaatgttta
     8281 gccgcagttt tttctttatc atcaccagaa aatgtagtgt taccacgaac acgaccaaag
     8341 tagtcataac ctaattctgt cgctaaacca aagttgtttt ggtttaagat ttggtaacca
     8401 ccgaatacac cgtaagttac agagttacgg ttaataccga ggttaatacc gaggttaata
     8461 ccggatccat aatcatatgt gttgtcaaat tgagttaaac cgtcgtggaa tgatgcccaa
     8521 ccagctttag cacctgcgta gaaagtgtta gcttgtggag ctgcttgagc tactgcagct
     8581 gctgataata ctgctaatgc aactaatgtt tttttcattt tgatgatcct cttaaatatt
     8641 gtcatcgatt aaaataaatt cttaataccc acaaaaagta ttaaaacttt aactttatgc
     8701 ttcaacatta taataagaag caag
//
LOCUS       DANLUA010000104         8650 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLUA010000104 DANLUA010000000
VERSION     DANLUA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..334)
                     /locus_tag="PXB44_001718"
     CDS             complement(<1..334)
                     /locus_tag="PXB44_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282177.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQISNANLIAPDWRKLLHLQNKELKFSKIGKNFFNKL
                     GSKELRKVL"
     gene            complement(361..1035)
                     /gene="cas4"
                     /locus_tag="PXB44_001719"
     CDS             complement(361..1035)
                     /gene="cas4"
                     /locus_tag="PXB44_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL5282178.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(1035..1898)
                     /gene="cas7c"
                     /locus_tag="PXB44_001720"
     CDS             complement(1035..1898)
                     /gene="cas7c"
                     /locus_tag="PXB44_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL5282179.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1908..3689)
                     /gene="cas8c"
                     /locus_tag="PXB44_001721"
     CDS             complement(1908..3689)
                     /gene="cas8c"
                     /locus_tag="PXB44_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL5282180.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3711..4388)
                     /gene="cas5c"
                     /locus_tag="PXB44_001722"
     CDS             complement(3711..4388)
                     /gene="cas5c"
                     /locus_tag="PXB44_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL5282181.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4630..6381)
                     /locus_tag="PXB44_001723"
                     /pseudo
     CDS             complement(4630..6381)
                     /locus_tag="PXB44_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6371..6832)
                     /locus_tag="PXB44_001724"
     CDS             complement(6371..6832)
                     /locus_tag="PXB44_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282182.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(6991..8127)
                     /locus_tag="PXB44_001725"
     CDS             complement(6991..8127)
                     /locus_tag="PXB44_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5282183.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8174..8590
                     /gene="tnpA"
                     /locus_tag="PXB44_001726"
     CDS             8174..8590
                     /gene="tnpA"
                     /locus_tag="PXB44_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL5282184.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
BASE COUNT         2367 a         1945 c         1634 g         2704 t
ORIGIN      
        1 ccaagacttt tcttaattct tttgaaccaa gtttattaaa aaaattcttt cctattttac
       61 taaactttaa ttccttattc tgcaaatgaa ggagttttct ccaatcagga gcaattaaat
      121 tagcattact aatttgagca atccctccta atacaaatac aggaggaacc ccaatgtggg
      181 aagaaatggt tctattaaaa ttactacctt ttttatcatt aggcgtatgt tttaacttcc
      241 caatatttcc accactctca tcaagaaata ttttgtagtt atgctgtgct atatttaatc
      301 cgtgaaatga tctttctaag tttgaagtta tcacaacaag tccctcccca aagtaggtaa
      361 ttataataaa gatccaaaca ctcccaccac ataccccttc gatctatccc tttccaacaa
      421 ctttggttgg cagaggtcga taagcgagca ggctttgcag cgtttgctgt attctggagg
      481 aggtgtggtt ccgctatcta agagcgaccg cacttcggca atggtggcga gggttttggt
      541 gcgtagttcg gtggaaaaaa tgatcggcaa acggtggcga gtttgcatat accacaacgc
      601 cccttcgtta atcgtctgct ctgtcatttc ttccaaacat aaggcttggg cacagagttg
      661 gatttcatca atcggatcaa gtttgggctt gccacgtttg tattccactg gttttagctc
      721 gcctgttttt aggtctcgct caaccaaatc caaaatgccg cttatgccta atttttcggc
      781 tgcaacgtga accgttcgct caaagcgaat ccctttgcga acttctggct caccactatc
      841 tacccgctcg tgcaacgctt gaccttgtgc ggtgagatag ttttctgccc acacctgttc
      901 gttgtggatc agcgcacatt gacgtgggca gaaggcatag tgttgcaaag cggaaagcga
      961 aattgaccgc ttgtcatcgc caaattttgg ctgaacaagc ggtaaaattt ccgcagaatt
     1021 ttgcaaatcg cccattacag taattcttca acggttacac ccgttaagcc ttcggtttgt
     1081 acgctgatac agtaatcgtc atagtctttc gctggcgtgt ctttttcacc gttgattcgc
     1141 tcaactttta ccgagtcaaa cagtttgtgt gcaggttggt tgcctaattc gctgtcgtgt
     1201 ttaaatacga tcagtttacg agctgccatt tcgcctcgtg cggcagaacg gtcgtgttca
     1261 aacataagtt gcaaggcttg ccaaagtttt tgtacatctt catcagaaaa gcccgttttt
     1321 tcagcgagtt tagctgaaat aaagccgtga acacgataaa gtgcgtaagg cacgatatat
     1381 ttacgcccca tagtccgttc tttttcaaga tctttttcgt tggtcactgc cattcgtgtg
     1441 atcgatactt ctagcggaat aatcggatcg atagattgag cgaacgccaa ttgcactggg
     1501 cctcgcacct gtccgctgtt tacttcggta gtcattaccg ccccaaaggt gcgaatatcg
     1561 aaaaagtttt tgcacatcca agcggtaatt tctcgggctt tagcttcctc ttttggtagt
     1621 tttttcgctt ccgattcaat ccctaatgct tcataagcac gtttgttttg taaattcaga
     1681 acactttttt ctttaacata aatttcgtaa cccgcctcgt tttcatagct catttcaata
     1741 aaattgcgga ttttgcgttt taggcaaaca tcggtgacta aacctttact ggtttcagga
     1801 tcaaggcgtg gcatatttcc tgcatctgga tcaccatttg ggttaccgtt ggttacatca
     1861 aagaaaaaaa caaattcata gcggttttga atagacatag tgtttcctta gttttcaata
     1921 gactcagtgg tttctttttt agcgaaatag ctttgttttt ggtggtaata accaatcgca
     1981 aaacgccctt gttcatcgat acttaaatgg cgagggaaat tttccggcaa gctggcaata
     2041 atctcaccta aatccttatc cagattaacc gccattccgc ccttatcttt tctcaagcga
     2101 gataaatggt gttttgcacc gcttaataag cgagggaata ccgaaaacgg cacggcagaa
     2161 gccgaaccat aatagcggtc ggcaatgccg gcatttaatt cgcctaatgc ttggctttgg
     2221 attctttcta atactgcaaa taacctgccg agcaagtaag cttggttatc gctttcttta
     2281 tttaagctca taggaactcc ttctttaata aaaccttttc taaattttcg ttgtaagact
     2341 gctttcatca acgctactcg caaaccgtta atatcgccgt ctgctcggat tcgagtcact
     2401 aactgtgaaa gtaaactcat cggatataaa tgaccgctaa taactgcgcg agccatttca
     2461 cccgccaaga ctggagaaat attctcactt ttacctaatg aagcggtttg taataatagt
     2521 cgccaaatgg aaggtggtgt tttccatggg caaggctcaa gagcgagatc ttgccaatgc
     2581 tcgctgaggt tttttgccag ttgcccaaag gtggtatcca gccaaaaacg taccgaaatg
     2641 cgagctgcat ttggagcaag ccccaacaca taaaaacggg tgttttcaga taattctggg
     2701 gaaatttctg caagcggtcg tcctttgctg attttttgca attcttcaaa gattttttgg
     2761 ttttcttgct catcatctgg aggcgtcatt aaagcagcaa aaaagccttc agcagcttca
     2821 gcttgggtgg tattgtctgc ttctgcccaa aagacagtgc tggcatcacc aatagttaaa
     2881 cgatgtttct cggtgcgtaa caaataattt aaagcagtag tgtaggcaaa ggcggcttgt
     2941 tcagaaacag gggcattact accttgttcc ttgccaaatg aagtgaagga ttctttattg
     3001 aaagagatga ttgaaccacc ggaactttgc ccaccaaata cccctttaat aggaggatga
     3061 gttcgttgaa ttggcgataa tttgcctgaa attaagcata aacctttttc agcattatca
     3121 ctttgattta tcaacaaact tgtccaaagg gtttgtgcag cttctcgctg atgaatataa
     3181 ccgcttgtgc cgactaattt aaacacgaca ttcgcatcaa gaatttctct tgggcagatt
     3241 gattcggcaa aatgtgtggg ttgccattgt tgcaggaaat ttcttatcgc cattaaacct
     3301 tcatcattgg aatctgcaag caaatccaaa tggtactgtt taaatgcctc aaaggttttc
     3361 tcggcgaaat tgatgggttg atctttggcg gtggctttat caggattact ttcaatgcct
     3421 agcacataag cggttttatc ccacagaaaa ttgggcttaa tgccagacgt gcgtttttca
     3481 gggcgaggta cgctcatcaa tttcggtttt ggtttcttat cttctgttaa atttgagatc
     3541 acattgacca aatttccctt tgaatctaat tctagtaccc aaccgatttt ttcttcactg
     3601 aaaccataag aaggcacttt agcttcgcct gttaagcgat cttcttgtgc tgcaaggcgg
     3661 taataatagc gagttaaggc ggtaataata gcgagttaag gcggagagaa tcatgatttg
     3721 acctcctctg cataaaatgg tggcacatca atcacgccat ttttcatttc cgcacggaag
     3781 aaacgaggtt gattaccgtg ctcaaaatca atgtcgtgca acatccagcc taaatcccga
     3841 tctcgctcat tttctgccaa aaaacactca ggtaacggtt catcgtcatc aatcaaggca
     3901 aaatttgcgg caaattcacg cacgcccata caaggctgct ggaaacattg cccctttttc
     3961 gcacggcgtt taaacatttc aatatgcttg gtgacggttt ccgcctcgcc tgccattttg
     4021 gtcagcactg cgtgagcttc gatcacataa ccgacatttt tcagcacagt agcagcacgt
     4081 tgttggcggt cgtcttcaat cacagtatat aaatccgcca ctgttttacg cttcattgca
     4141 ccactgattt tactggcaga aattttactg cctaactcat tacggcgaac agactcaaat
     4201 tgaatcggtt gtaatacata aattttatca atcacccagc gaattgccgg cttccaatgc
     4261 accgcagcca aaatcccccg tgccgcagac ggcgtaatca catcataaga cacccgttct
     4321 accttcattt caggacgggt aaaacaagca taatcacccc aaatatggag cctgattttg
     4381 tttggcataa tagaatgctc cttttaatta tgtaacacac acctccccga agggaggtga
     4441 aatttcttta gcttatatta taactgcttt ggtttcaacc cacgcactca gtaaaatgcg
     4501 aaaaatttta tggcttatta tattaagcgg tcggatttac ttagaaagtt gcaaagttat
     4561 ttgagtattt aaccgcttgt atttcaaaaa tagtcagaaa aataacagca ttctaggtac
     4621 aatatttttc taagaacgaa cttgtctccg caatgcctct actgcgtttg acacagcatt
     4681 tggtttgggc tcctggtttg cccaatcaga ccagaattgg cgtgtagcac tttctggggc
     4741 tatatgcggt ttagcaagtt tgattcgtgt aggtaataga atcgaatcgc ctagcaataa
     4801 cgcttcgcct aaatcaagta acggcaatat atcagtaatt ccaaccatag attccggcaa
     4861 taaacgcttt acaacacttt gatcttgacc atttgttaat cgtaaaacca agaaattatt
     4921 acattgactc aaaattgttc ggcttacatc tgaaggacgt tggctaacga ccaacaaaga
     4981 cacgccatat tttctacctt ctttcgcaat ccgctcaaag gcttccaatg cgcgtttttc
     5041 cgctgcttcc gctgaatctc gaacagggag atataaatgt gcttcatcgc aaataatcgt
     5101 tagtggatgg cgggaatctt tatccatcca aaattgaatg ttgtataaaa ggcgggcaag
     5161 aactcccgta acaatcggta aaacatccga cgggacttcc gaaaaatcaa taatttttat
     5221 ccctttttcg ctctcagaag cagaaaacag ttttgacaac atattttcca gccacccata
     5281 gctgttgcac tctactggcg gttggaacat aaaaccgcaa cgtctgtcat ctattttggc
     5341 tttcatcctt cccataaacc tagacaactt actataccaa ggaccttgct ttgttcctga
     5401 acctccctct ttaggtacca tctcagaatt atctttatct aaacgagcta acaataattc
     5461 aatagaatac ggaattggcg aatcaacagt aaacgtttcc aaaacatcat ttctttcttc
     5521 cttattcaat gtttgctctt ttaatgcgcg aacatgctca gtaaaacgcg ccgcctgatt
     5581 cggagcatta tcatcactgc gatctaaaat taaagccagc atttattccc gattgagcag
     5641 ccaataggga ataaataaac tgcgttcatc tgaaccatcc aaatctcccg gtccagcaat
     5701 acggtattgc tgagcgatag cgtttttacc ttcgcataga gtgctatatt caccgtgcat
     5761 atcgaataca ataagattag cagcttttaa agctgctgca cgttccaata ttaatgcaac
     5821 tgaccagctt ttaccggaac cagtactacc caaaatagca gcgtgccgtt gaaagaattt
     5881 atctccgttt gctatagcta cagccgtttc atcagccata aaatgaccta aagtcagccg
     5941 ttcattttct tttacgttag cggacaaaag attcataaat aattgcagat tgctgccact
     6001 aatatgaaag caatctctat caatttgcgg aaaacaatcc gctccccgct taaaagtgtt
     6061 gctcaaacta ccatcaacag ctttataagt accgattaat actgccttga taaagtcatc
     6121 ctgactttca cccagaggaa tttcaccctc attagatatt tcttctagca gcaatttttc
     6181 actgatagaa cgcgttaccc gttcgataat gccgataagg tactcactag ccgttctgcc
     6241 ttgaatcgca atcagattac ccacagcaat actagtcatt tgttgatgct cttcaacaga
     6301 aatcaataca cggctggtat ctactgttac aaccctgccc aaacgggcat tgtctttaaa
     6361 attcaatgtt tcatttgcca ttctctaaaa tctcttttac aaaaaactca acgtcccacc
     6421 aatgaacatt aggaataaaa gtttcttctt gcttgctaac cacccttgtt ccagccttat
     6481 ctccatcaaa aatcagagcc ttacaattcg gagcttcctt caccacgttt ctggcatttt
     6541 ctgaaagtcc tcttgtcaga agtagtgttt tcacaccact tttaattttg cctgtcaaat
     6601 gtgtttgaag gtggtcatca ttaaaaccat agccgataca taaaattgaa gaagcagaat
     6661 caatagcttc atttgctttt tctcgatgag catcaaatgg ttgtccatag ccttttttat
     6721 acttcccaac accaggcgta ataattaaag gctcttccac tttacctata caacttgtca
     6781 ttggaacgtc tccagccata aaccaatcta aactaccatg cggtttaaag attttgatat
     6841 gatctcgttg acatcctccc ctgcctaaag gcaggggatt cctactagct cccacagcaa
     6901 gctgtgggct actctcggtg ggtttctact gctgatctct attgagattc acttcataga
     6961 cgcttcgggt atgtcctacc ctgatattta ttatgcttgg actattgctt gcccacgtgt
     7021 aagaacattc attgctccca ctacatcggc attttccgta tagccacatt ctacgcattc
     7081 aaaattttcc tgtgtttggc gattttcttt tgctgtatag ttgcaacaag ggcaagtacg
     7141 actggtattt tgtggaggaa ctgctagcaa aaatccgcct aaccattgcg ttttgtagtc
     7201 cagttgtcgt ctaaattcaa accaagactg gtctaatatc actcggttta agcctgattt
     7261 ctgtttcaca tttttgccgt gtacttccgc tgtgcctttg gctgatttgg acatattcgc
     7321 cacttgcaaa tcttcaacat agatcatcgc gtggtttttg ctgatttgcg ttgagatttt
     7381 atgcaggaaa tcttttcggc agttggcgat tttgtgatgt aacttgccga ttttatcttt
     7441 cagtttttgc cagtttttac tgaacttcac tttatttttt agctgttttt gcagttttgc
     7501 caattttcct ttgtgggttt taaacgcatt taacggctca aaaaattctc cattggaaag
     7561 ggttgcaaaa cgagcaatgc ccatatcaat gccgatttct ccgcctttat gcgtgggaat
     7621 ttcctgttta aattccgttt gaatgctcac aaaaaagcgt ccgcatttca ttgaaacggt
     7681 gacatttttt acttccccaa caatttcacg actgttgcga taacgcaccc aaccgatttt
     7741 cggcagatac aagcggtcgt ttttctgctc gattttgcaa ccttgcggaa aacggaaact
     7801 ctcttttacg ccttttttct tgaatttggg caaatccgca cgcttttgaa agaagttttt
     7861 aaacgcactt tcaaggtctt ttaacgactg ttgaagcact tgtgaatggc aatctttaag
     7921 ccataccaat tcttttttcc attgcggaag caagttggcg agcttggtat aactgaattt
     7981 gtgatggtta tcttgttcgt actgctcatt ttgccacgcc aatgccttgt taaacacaaa
     8041 gcgagaacag ccgcaaaact gcttcatttt gcgaacttgt tccccatttg gtctgatttc
     8101 aaatttaaag gctttacgta gtaacattgc acgaattaga atacaaaaat tgtgcttatt
     8161 ctacacttgg cctatgaaaa aagaaacaga gattagacgt ggtcgccacg ttgtttttaa
     8221 cttacacgtt catttagttt tcgtgactaa atatcgcagg gaagttttta ctcaagcaat
     8281 tttgaacgac ttacactcaa tttttgcaac gatttgtgct gattttgaag ccacattagt
     8341 ggaatttgat ggtgaagatg atcacgttca tctacttgtg gaatatccgc ctaaagtcgc
     8401 tatttctcat cttgtaaaca gtttaaaagg cgtttcgagc cgaatgatta gaaagaaaaa
     8461 ctatccgagc attcgcaaaa aattatgggg aaatcaactt tggtcaccgt cttactttgc
     8521 tggtagttgt ggtggtgcac caatatcgat tattcgccaa tatatcgagc aacaacaaac
     8581 acctgattag ttagaaattt gaaacggctt tgccgtttat accttatatc cacgccctaa
     8641 aggacgtggt
//
LOCUS       DANLUA010000105         8485 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLUA010000105 DANLUA010000000
VERSION     DANLUA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(75..539)
                     /locus_tag="PXB44_001727"
     CDS             complement(75..539)
                     /locus_tag="PXB44_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5282185.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            complement(523..726)
                     /locus_tag="PXB44_001728"
     CDS             complement(523..726)
                     /locus_tag="PXB44_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282186.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            complement(723..1250)
                     /locus_tag="PXB44_001729"
     CDS             complement(723..1250)
                     /locus_tag="PXB44_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5282187.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            complement(1328..1780)
                     /locus_tag="PXB44_001730"
     CDS             complement(1328..1780)
                     /locus_tag="PXB44_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5282188.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            complement(1801..1977)
                     /locus_tag="PXB44_001731"
     CDS             complement(1801..1977)
                     /locus_tag="PXB44_001731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282189.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            complement(1990..2625)
                     /locus_tag="PXB44_001732"
     CDS             complement(1990..2625)
                     /locus_tag="PXB44_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL5282190.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            complement(2622..3416)
                     /gene="bet"
                     /locus_tag="PXB44_001733"
     CDS             complement(2622..3416)
                     /gene="bet"
                     /locus_tag="PXB44_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL5282191.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            complement(3457..4407)
                     /locus_tag="PXB44_001734"
     CDS             complement(3457..4407)
                     /locus_tag="PXB44_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282192.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            complement(4420..4632)
                     /locus_tag="PXB44_001735"
     CDS             complement(4420..4632)
                     /locus_tag="PXB44_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282193.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            complement(4766..5245)
                     /locus_tag="PXB44_001736"
     CDS             complement(4766..5245)
                     /locus_tag="PXB44_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282194.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            complement(5242..5601)
                     /locus_tag="PXB44_001737"
     CDS             complement(5242..5601)
                     /locus_tag="PXB44_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282195.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            complement(5605..6111)
                     /locus_tag="PXB44_001738"
     CDS             complement(5605..6111)
                     /locus_tag="PXB44_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282196.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            complement(6298..6373)
                     /locus_tag="PXB44_001739"
     tRNA            complement(6298..6373)
                     /locus_tag="PXB44_001739"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6338..6340),aa:Arg,seq:tct)
     gene            complement(6680..6916)
                     /locus_tag="PXB44_001740"
     CDS             complement(6680..6916)
                     /locus_tag="PXB44_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282197.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            complement(7298..7528)
                     /locus_tag="PXB44_001741"
     CDS             complement(7298..7528)
                     /locus_tag="PXB44_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282198.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            7820..8095
                     /locus_tag="PXB44_001742"
     CDS             7820..8095
                     /locus_tag="PXB44_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282199.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            8104..8409
                     /locus_tag="PXB44_001743"
     CDS             8104..8409
                     /locus_tag="PXB44_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL5282200.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
BASE COUNT         2138 a         1705 c         1691 g         2951 t
ORIGIN      
        1 aagaagaaac cgaaactctt atagagtggc ggaatgcttt tctgcaagat gtttcggttt
       61 ttatagctta ggtactataa atcgtcttct ttaacccaaa tcccagcttg catttcgccc
      121 ttgcgatctt tgatttggtc gtaggcgtga gcgaggcagt cattaagcgt aaatctattt
      181 attaaggcta aatggtgtaa tatgccaaaa aaacggctat atttacctct gtctttaatg
      241 cgttcaatat catatagaca atgaacagcg accgaaatat caaatctgta accatttaat
      301 ctatgatttt caaattgtac agggtaaata ggttgttttt cttgttttgc tttaataata
      361 cagacaacaa tcaaatcccc aatcgcatct ttaatcattt caggattatt gcgggcaatg
      421 cctccgcaga gttcgccaaa ctcttccatc agtttgagca tttgcttttg tggggttgag
      481 ccgttaatta aattgcggtc ttccgcccat tgttcgatgt ttttaattag ttgttgcatt
      541 gtttagtcct ctcatttctt tcattgcttc atcaatcgcc gtgcgtaaat cggcatttct
      601 tgcccaatga aaatactgct cgtgcggttt ggtatagcta gcaataataa attcgtccac
      661 caaatcagaa tatcggacat ctaatttgcg ttgggcgata aagtctaagc gttcggtgtc
      721 ggtcattttt cctcctttgg tggttcggga agtggttgcc agtgggttac tgcaatcaca
      781 tcgtcatcaa gcccatattg atttacacta agaaaatacc actcgttatc ggcaagttct
      841 cttgaaacta ttaaaattaa ctgcattcct gaaatatcgc ataaccctag cacatccgtt
      901 tcaataggtg gtaatttgtc ctcaatggaa atccagccgt tgttttctaa aagtgttggt
      961 ggttcaataa cttgttcagc ttccccaaat aactccgatg ctctttcttc gtctgttagt
     1021 ggacgaatat ctgattttgc tctacctaaa acaacaccat aaactgcata gggtaaatca
     1081 taggcttcat agctttcata gctttcgtga tcatccatgt cacccgcaaa ctcataggct
     1141 tcagtagcac catttaaaca gctttgtttt gcttgctcta atgtttcgtg caaccttatt
     1201 acatgtgtct catccgagac atcgaccgaa aaatattttt cttcattcat tttctttctc
     1261 cgcgttccgt gtttatgtgg atatacaaga aattcaacag cagcattcag ctgttagtgc
     1321 tgccgattta cctacgacta tttcgcattg catctaacca ttcttgagcg tcttcttctg
     1381 aagcgaaaca tcgcccttgt ggcaagaatt tttttacaaa gggggcattt gcgaacatag
     1441 attttactat ggtaaaatca ttgtcaataa accacatatt ttcttgaggt tcttttaatg
     1501 ggcaaggtaa cgtcaatgtt actgctggtc ttggttcttc ccacatacca acaatatcat
     1561 attgagctat tgttccatca ttgaaatgag atccgctaac agcccacgat acttctgaca
     1621 aaaatacatt ttcttcatca actgtatacc cttgtacttg gtggtcttta ttatctctaa
     1681 tatagtcatc actaattagg tattttacaa atgccttatc attatttctt agttttactg
     1741 gctcacctgc caatgcttta tttaaatcaa atggtttcat tttattctcc gtgtttgtgg
     1801 ttatgttgat gtacaggcaa ttccaccata gcattcagcg gctgtggcgt gagtggctct
     1861 tgcggaatat tcagccgtcc gcctaaagtg gcatatggct ttgatgcggt agagtgttct
     1921 gccgttgtgg ttgattgtgt cgttttggag tagttcgtat ttcttttggg ttgtcatatt
     1981 ggctacctat taaaaagccc ctttcggggc ggttaccccc ttcaatgcat aaagggattt
     2041 gataaattgc gggatatatt gatcaaaggc tttcatcaat accggatcac gttcggctgt
     2101 gtagatgtaa agcggttgtt tttggtattc ggggcagtag ctgacaaagt cccacgtttc
     2161 gtagcctgtt acccaaaggg cagattgcac ttgaataaga tattcattcg gcacaccgcc
     2221 ttctagcagg tatttgatgt gggttttcat tttcgggcat ttgatttcca accctttttt
     2281 tagattggga attaagccgt caggacttac cattaactgt ttttcggcgt taaggtacac
     2341 accgccgact tgcacgactt cggtgtcggt ttcaaactcg taagccattc gtgcaagcgg
     2401 ttcaagctcg ttgcctcgtg ccatatcaga cgacttaaaa ccgtcttttg ctccctcaat
     2461 gctttcggca accagttcgg caagatagct attccaagcg gacgattttt taccgcttgg
     2521 cgtaacgata ttttcaatgc ctgtggcggt gggaatgccc aatctggcat tgagccattc
     2581 ttctgatcct tgttcgcaat ctaaaatgat taaatcgcct gtcatagtgg gatttcctca
     2641 ccgacacttt cgccattatc cgccttttca gcgttttgct tgtctagcgt aagattgagc
     2701 tttttaatca ctgcctctgc ctgcgattta gggatttggt tcagtgcttg aacgccaata
     2761 taggcgagaa ttttcgcctc ctccgtaccg gttacttgca ttaactgccg gatttgcgac
     2821 agttgttctt ccgaaatgag ttcaacggca gtggtttcta ccacgttatt ttgtggtgtg
     2881 atgtttttag gtggtttttc attttccgca atacgctctg cttcgtcttg gtcgtaaata
     2941 cccgtaaagc caaaggctaa tcttgcacat tggatcattg ctttatggcg taacattctt
     3001 tttgggtgag agttccaagc tggagagctc tttttacatt cccccataaa ttccgtcacc
     3061 acaatcggtt tacttctatc tttacggtag atagtacaag tacaggcgga atagtcatcg
     3121 gtaaaatgaa attccattcc atcatattga ggctcacggt taataatgcg aacccaacca
     3181 tcaacgccga caataggcgt taatccaccg ttcccaccgg ggaatgcata aatttctttt
     3241 gtccaagggt ttaattggta ttggttagca ataaccaata gtgcaatcat ttgagcatcg
     3301 cttacggttg cccctgcgaa agcagacgat ttaagtgtct caactaatcc tgagccatct
     3361 cccatttcaa aacgctcggc aagtttgttg gttagtgttt gtaatgcagt tgtcataata
     3421 tgatccttat ttaggttgaa tttgtgcggt atcaaatcat ttagccttat tgagcgaaac
     3481 gctatcccca aatttcgctt tcagctcacg agcaatactg actgcgttgg tttggattgt
     3541 ttggtttaag cgaatagtaa tgataaaatc gcctaatggc ccatcggttt gtgccggttc
     3601 ttcgatgttc atttttgcca cattttgcgg attttcgacc gcttgtttgc gctccatttt
     3661 atcagtaatc gctttggctt cggcttgtac tttgtcggct tctgctttgg ctttaatcgc
     3721 cgcctcacgc tcggcttctt cggcaaggcg ttgttgaata attggggcga ggtcgtcttg
     3781 ggttgcgatc aatttcactg catcaggaaa aagataagct gattttgcag caagctgttc
     3841 taatcgctca gtcaagcgcc cgatttctac cgacatttcg ctgataatca gcgctttttc
     3901 ggcgttcacg gctttggtta agctgtcaat cgtgcgttta tttttctgtg cctcggcaat
     3961 acgattagca atgccgtgtt ttggcattgt gatttctaag gcaagggaaa tatcacttac
     4021 tttaaccagc ttgtttctgg tatcagtgat ttcttcaatg gctttatcgg caatctgttt
     4081 tttgatttcc gtttctttgc tcttaacgag cttgtctcgg gttaatcgct cttggcggaa
     4141 acgttcagcg atttgttctg ctgttaatac caattccgca atctcgccag tttgcgtttg
     4201 tttaattgcg tcacgaattt ttttctcaat gctttcaagc tccttcactt cttcttttgc
     4261 ttttgcaaaa tcatcatcgg tttcaaaggt ggtggtgagc gtggcaaggt attgatttgc
     4321 ttgctcttca aaagtttgca agttggtaga aaccactcgg ctttcggttt ttaaaataag
     4381 ttcaaattgt tgatttgcag ttgtcatttt cttattcctc taaaattcat ttctgtgttt
     4441 gttctgttcg gctaggtagg cgttgattgc gttacgccag ccatcagaac cggctaggat
     4501 ggtggcaatg gtttcaaagc cgttgttttc taatagctcg atgatgtttt cttcagccaa
     4561 gatgaaagcc tcttcgctag ggtcttcttc tggttgaagt gaagcatctg tgtagtcttc
     4621 gtatcctatc atcacacttt ctccgttact ttaaattcgc aagtttcagg cttgcaggtg
     4681 ctttcaggga taagctcttc attgcattag gagggtgatg gcaaccataa aaagcagggc
     4741 gtataagccg tatttaatcc attgtctaag catgtttagc attgtttgcc tcttgtgtac
     4801 gttttaccat ttcggcaaga acgttgaaga aatcagtttc aagggtaatt gtttcggcac
     4861 ttgctcgtct gtctagatgt aagcgaatat taccgttgct atccacaaag tagccgttta
     4921 gcccataagg tatgaatggc ttgcgttttg gttttgatgg tttaggctta acctgttcag
     4981 gatcttcctc aatttgttca aaattgtaat tgtacataat aggctgatga ccccggattt
     5041 caggataatt aggcaaatta acggctttgg ttttattcag agcttcaatg cgcttattta
     5101 ctgccttaat agcgttaatt tcagacatca tctctgccgt gctgatttct tttatgccgt
     5161 ttaatataac ttctccgaag tagcgttttg tatgattatt ttttaataag cggatagtat
     5221 agctttctac tttattgcgt ttcatttcag caactccttg atttggctaa tacgatactc
     5281 aatcacctca agtattccgt cttgcttctg tttgcgttgc tcaaggtttg ataattcttc
     5341 tcttaatgct tggcggttga tttggtgttg cataaatgca ggtgaatcaa tttgtttcca
     5401 ttgttctaca aaggattttg cctgttcagg gcacttgaag cgtttgcgta tccttgaaat
     5461 ctgcacccac acatcattga ttttttgctt tatgcggata tccgctctcc agcaatcgtg
     5521 gcgtttatgc ttaatgttgt ggcgaaagcc tttcgtggag ctgtcgtgcc atacactggt
     5581 ataaatacta aacgtcgtca ttttttactc cttgtgaata ctaaattcat tcaaacaatg
     5641 cttatttttc aagaattcat ctaagcactc atcaagagtt agctcgtatt ctttaagaat
     5701 gccatttagt tcaatcagaa tcccagagca tttgttaaat aaattaatcc tgattgtatt
     5761 ttggttttgt tctttttcat tgctgtccac taaccaagga gcaaaccaag caaattgcga
     5821 ggtatgatgt aacatacgta tcgctctttg ctcgggtggg gttgttccac tagctttcca
     5881 cgtgtgaagc tctaagtctt catcgaactg gatttgatgg ttaatgatcg ttagtagtgt
     5941 aaaaatctct ccagcagcaa gtttaatatg ttgttctgca ttattagcta ccccgtagca
     6001 cagtttccct actgatgtag cgagatacag acaatgtttt tgagtaagct caatatgggt
     6061 caaaccctgt tgttttgctc gctcaataat aattttgtat ttagttttca ttttgaatcc
     6121 ttttatgtgg ataaaaaaag ccccgcacga ggcggggaaa cggagtcatt atgtttatta
     6181 tcaaccgctc tttttaagtt gtagcagtta cacaacttag ccattcaaaa ccgctctaca
     6241 cggtaggttt ccacttcgta ggctcgtcat cgatgatgtg cgtaaagcgg ttttggatgg
     6301 cgaccgcaga gagattcgaa ctctcgaccc actgcttaga aggcagctgc tctatccact
     6361 gaactatgcg gtcaaagtac cgttgcttaa ccccaacggc agggcttact tcaatctaag
     6421 gaatgtgatg gactaaccat ctctaacaac tctctagtac attcgtttcg ctgcctaacg
     6481 ccgactttag aaaacattaa aactaaaacg gggttatttg tctcactctc tgttgagagc
     6541 tgttagagat ggtggctctt ttttatactt gtgagccacc gggccaagtc ctcttgtttg
     6601 ccacaacttc gaggaatata atacttgggc aacatctagg gctaatgccg aaaaaatgaa
     6661 atcactttgg tgttattgat tagttatttt ttcggtattt atcaccattt ttcgtatcta
     6721 aaagctcttc tgtaatacag ccatcactaa atttattgat ggctgatatt gcttggcaaa
     6781 ccgctatttc cagccccaca taatatggcg ttatagagac ggattttaat attgcctgcc
     6841 taatatcttc tttctgtaat tcatcaatcg ttgaaggttc aacgctgcct aatttaacaa
     6901 attcctgatt actcattttg ttctcctgtt attaatattc caaagcacac tttctcaaat
     6961 atgctttgga attgaaccag cgtttgtgct tcccgattca ctgccagtgc ttaacctttc
     7021 cgctagttcg cacatcacca gtaaattcgc cacaactacc gtactattct ttcagtccga
     7081 gtagcttaga gagtttcaca acttgcctat aagttcattt accgcatacg ttttaccgct
     7141 ttatgcaaag attggttctg tctctccaat gtcattacat tgctaagtaa tccgcttgtc
     7201 gtgatgtgtt tttggtttaa tttttaaaga acaatgcctt tcggcgaggt ttagaacctt
     7261 tattcaagcc ctccgtgaag ggctttgata aaaattctta gtgaatgtag gcttgccaaa
     7321 gttgttgagc ttgctcggcg ttcattttgt cgctttggtt ccagtcgatt aacttgccat
     7381 ttctgaacat tgctctttca atcacaaact cttgcgtggc ttcattttca aagacctcaa
     7441 acacattgat gaaatcactg gtgcgttttt ctttgcgtcc gcataaaatg tagtctttgt
     7501 ggttatttag cgttgcttgt gcgttcattt tggtttcctc tttgtctgtg gcgttgtttg
     7561 ttttgatggg tttattatca caaattgaaa tcattaagtc aattcaattt gtaattattt
     7621 tagccaaaca aatttcaatt cgtgattaaa taattgattt taaagagaaa ttatttttag
     7681 aaaggttgtt tgattgcttg ttttttaaac acttaacata agaagagaaa gacaattagg
     7741 agaaaaagca cttaaaaaag attgtctttt ttattgtgcg tgatatattt gtatcacatt
     7801 gtgtttgcta ggtgttatta tgattaagag cttcaagcat aaaggcttaa aacaatattt
     7861 tgaaaaagga ataacaaaag gtattcagct taatcatcaa cgtaaaatcg atggtatttt
     7921 agatttgatt gattcagcag aaagtattga tgaatttatg cctttttacc aatgccacga
     7981 gttaaaagga aatcgaaaag gcatttattc tatgacggtt aatggcaact ggcggattac
     8041 atttgaattt gtaaatggcg atgcttatat tttgaattat gaagattatc attaggggga
     8101 gttatgcgta aaccagcaca tccggggcaa attttattag atggatttat tgaaccaaat
     8161 aacatcaaaa ttaaagaatt agcagatcat cttgggtttt ctcgtgaaac tttatcaaga
     8221 gtattacacg gcaaaacacc aatgactgca aatttagcgt taagtttaga agaggcagga
     8281 attagtacgg ctaagttatg gctaaactta caaactaaat atgatttatg ggagttaaag
     8341 cagcagcggt taaatcctgt tatgcctttt cattttggtg gaaattatgg agaaactgct
     8401 cacgcttaat aatgccctct tcggaggatt ttattgttct taggaaaaga aaaccgccac
     8461 gagggcggtt tattcaagga atatt
//
LOCUS       DANLUA010000106         8363 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLUA010000106 DANLUA010000000
VERSION     DANLUA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            9..3167
                     /locus_tag="PXB44_001744"
     CDS             9..3167
                     /locus_tag="PXB44_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL5282201.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIHGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXB44_001745"
     CDS             complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXB44_001745"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL5282202.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6264..6545)
                     /locus_tag="PXB44_001746"
     CDS             complement(6264..6545)
                     /locus_tag="PXB44_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL5282203.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXB44_001747"
     CDS             complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXB44_001747"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL5282204.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7001..7558)
                     /locus_tag="PXB44_001748"
     CDS             complement(7001..7558)
                     /locus_tag="PXB44_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL5282205.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7567..8316)
                     /locus_tag="PXB44_001749"
     CDS             complement(7567..8316)
                     /locus_tag="PXB44_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5282206.1"
                     /translation="MSIKFVDLLQQSWNFMRNQQAFSLFAIVTIVVVQLVFILVSPNS
                     PELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINVLMIFNIQSINDGNYRQFFQN
                     AGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVIIALPVLVVGFYFFIKLSLVIY
                     AYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYLLPGMLSRLFTVFGNDFIGIF
                     ITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
BASE COUNT         2460 a         1824 c         1792 g         2287 t
ORIGIN      
        1 gagttgtgat gattaccatc aacgaaaaca ccattgaaca atccgccatt gcaactttgc
       61 aaagcttggg ctgggactac acctacggca aaaaaatttt ggcaggttta gaacacgaat
      121 ggcgggagcg aaccgctgag gtgattttaa agccgctttt ggcgcaagcg attgcaaaat
      181 tcaacccgaa tttgcccgct tgtgaggtgg aaaatgtggt ggcacaggtg tgccgtgccg
      241 acagtggcga tttggcggag cgtaatcgtc aggcttatga ttggctaaga aatggggtca
      301 aaatcactta tcagttgtac ggtgagcagg tgtctgatgt ggtgcagctg attgattttc
      361 agcgtccaga aaataacgat tttcgcattg tcaatcagct ggatattagc ggcaaaaaag
      421 gcaaacgcat tccggatttg attggctttg ttaatggctt gccgctggtg gtgtttgagc
      481 tgaaaaatcc gctcaaagaa aatgccgaca ttggcaaggc gtttgcccaa ctgcaaacct
      541 ataaagatga aatttctgat ttgtttgtgt ttaatcaggc actcgtgatt tcagacggca
      601 ttgtcgcccg catcggttcg ctgaccgctg atttcgaccg ttttacccct tggcgtgtgg
      661 ttgatgaaaa aaatcagagc aaacgcattg tgtttgaaga tgaactcacc gccctgctgc
      721 aaggcgtgat gacacccaaa aatctcttgg attatgtgca gaattttgtg gtgtttgaac
      781 gggacggcaa aaaccgctta attaagaaaa tcggagcata tcatcagttt tatggcgtga
      841 atgaagcggt ggattgcacc ttgcttgccg ccacaggcaa ccgcaaaatc ggcgtgtttt
      901 ggcatacgca aggttcgggc aaatcgcttt cgatgctgtt ttatgcaggt aaagtattaa
      961 gccaaagcag cctgaaaaat ccaactttgg tgttggtaac cgaccgcaac gatttggacg
     1021 gtcagcttta cgccactttt tgcggtggcg aggcactgct caaacaaacg ccaatccaag
     1081 ccgatgggcg agacgaactc cgctctgccc ttgccagtcg ttcggcaggc ggtgtgattt
     1141 ttaccaccat tcaaaaattc ggcttaatgg agggtgagct tgcccaccct gtattaaacg
     1201 agcgggaaaa catcattgtg attaccgatg aagcacaccg ctcacaatat ggttttagcc
     1261 aaaaaatcaa ccacaaaggc gagtatcgtg agggttatgc caagcattta cgcagtgcat
     1321 tgccgaatgc ctcttttatt ggctttactg gcacaccgat tgaacttgat gacaaagaca
     1381 cccaagaagt cttcggtaaa tatgtctcga tttatgattt tgaagatgcg gtggaagatg
     1441 gggcaaccgt gccgattatt tatgagccac gccaaatcag cttaggcgaa agtggcgagt
     1501 tttccaaagt gatggaagag gcacaacaac tgattgatga cgatgaaaac agctataact
     1561 tccgcctgcg tgaaaaactg cacagcgtgg atagccgttt gcaaaaaatg gcggaagata
     1621 ttattgccca ttacgatgag cgtaccaaac agcaagacgg caaggcgatg gttgtggtga
     1681 tgagccgtgc catttgcgtg aaattgtacg aaaaaatcac cgcacttcgt ccagaatggc
     1741 actcaaacga tgtgcttcaa ggcagcatta aaattgtgat gacaagtaat gccagcgacc
     1801 ctgccgagtg gcaaaaacac aatcaggata agaaaacctt agaaaaacgc tttaaagacc
     1861 cagacgatcc gctcaacatc gtgattgtgc gggatatgtg gctgacaggc tttgatgcgc
     1921 cctgctgtaa cacaatgtac attgataaac caatgagcgg acacaacctg atgcaggcaa
     1981 tcgctcgggt aaatcgggta tttcgcaaca aaagccgtga aaatggcggc ttgattgtgg
     2041 attatgtggg cttgaccgat gagctggaaa aagcgatgaa gcagtacacc aacgcaggcg
     2101 gtaaagaaaa gccggtgcgg gacatttcag cggtgctgga aaaaatggtg gaacatatca
     2161 cggtcattca tgggcaattt gccacaccga ttgacggaca agcggttgat attgccaaaa
     2221 tgttgcaaat cagcgaaccg cctaaactgc tcaatgcgat tttgacatca gccaaccata
     2281 ttcttgccct agaccgcatt cagccacctg ataacactgc caaagacaaa accccacgca
     2341 aaaacgcctt tttgcaatcg gtacgcttgg cgaaaaaagg ttatgctttg tgtggggcat
     2401 taaaagcagt tgaaccctat aaacaggaac tggcgtttta tgatgcagtg cgtgccacca
     2461 ttatcaaaaa cagcaccgct cctcgcaatt cttcgagcga aaatgaccgc ttgttgcagc
     2521 ttaccgcctt gatgaatcgt gcggtacagt cggatggtgt ggtggattta tttgatttgc
     2581 tgaaaaaaga ccgcccaaac atcaacctgc tttctgatga gtttttggaa acagtaaaaa
     2641 acagcccaac caaagatttg tggctatcag caatggagcg ttatcttgcc tcacaactgc
     2701 gagagcaaag cggtgccaat cttgccacaa aaaaagcatt tgaacagaaa ctcaaagagg
     2761 cgatgaacca ataccacaac cacaatttaa gcgtattgga aattttagaa gagctgatcg
     2821 ccctcgccaa agagtttgaa gcccgccaaa aacgagggga agcgttggga ctaagccctg
     2881 cggaaatggc gttttatgat gctttggcac gtaatgaaag tgcggtgcgg gaaatgggcg
     2941 atgaggtatt gatgaaactt gccaaagaca ttaccgataa attacgcaaa tcggtcaccg
     3001 tagattggca atataaagac tcggttcgtg ccaaaatgcg aaccttaatc cgcattgctc
     3061 tgcgtaccta taaatacccg cccgatttac aggcggaagc gattgagttt gtgttgcaac
     3121 aagcggaaga gatcgccgga gagttcgcta tcaccgaaac ggcgtaatct atattagggc
     3181 gtgcaatgta cgccctaaat ttatgctaat aacctctgcc acatttcccg cacaaatgct
     3241 cgctcggtgg taaattcact tgcgtccacc acgctgtctt tgcgtaataa gtttaggtgg
     3301 tggattttgt tacgcagtgc ggtgtagcaa tgttgtagct gttcgctttg ttcggaactt
     3361 aaaataccgc attctactgc gctgtcgaaa attcggacat tatccgacca tactgccatt
     3421 ttcgggtgtt ggtggctgtg ggctaatacc aaatattggg cgataaattc aatatcggta
     3481 atacctcctt gatcttgttt aatatgaaat tgctcggctg aatgcgagct taaatgttgg
     3541 tacattttct gtcgcatttc gcaaatttct tggcgtaatt gaccgcttgc acgaggctga
     3601 gcaagcgtgc tttggcggat tttttcaaag gcttgtttga ggttttccgc cccaaataca
     3661 catcttgtcc gcacgagggc ttggctttcc cacgtccacg cttcattttt ttgataatgc
     3721 tcgtaggcat tgaaagtgct gactaaaagc ccagcctcgc ctgatgggcg aagtcgcata
     3781 tccacttcat atagtacgcc ggcactggta ttaaggttaa aaatcgagtt aattttttga
     3841 gccaatttta agtaaaattg atggcttgaa atctcttttt taccgccaac agtttggcta
     3901 tcctctgggg cattatgcaa gaaaaccaag tcgagatctg aattatagcc cagttcaatc
     3961 ccgcctagct tgccgtagcc aataactgca aacccttttt catcagcctc taaatgttcc
     4021 ggcacaccaa agcgttggct gacttgcttc cacgccatat ttaccaccgc attaataatc
     4081 gcttctgcta agtaagtaag atgatcgctg atcttcatta ccagcagcac gcctaaaata
     4141 tcagcagcgg cgatgcgtaa aatttgggtt tgtttgaact gacgtaagcc atcaatcagt
     4201 gcttcttcat cttcttcagg aatgcggatt aaataatcct gcaaggcaga ttgatatttt
     4261 tcaaaagcct gcacgtcggt taagcctcta tggctcatca gctcatcaag tagcattgga
     4321 tatcgagcta tctgttccgc aatcataacc gacttactac acaatgctaa aagttgaggc
     4381 agaatctgct ctttttcgag cattaattcc aaataggtag ttcgggtggt gattttatcg
     4441 acaatattca acacgcgagg caataacaat aaatgatctt cacctttaaa aatgctgtcg
     4501 gcaattctag gcattaaatt gcgtagcacc tctcgccctc ttaccccaat cgggcgtttt
     4561 acccaatctt gaaaggtagt agaaaagtga cggaaaatct ccgtataatc attttctgca
     4621 acctttggat aaccttttag cactgcggat aaatcttcaa ggcgaatgtt atagtgcaaa
     4681 atgtctttcc attcggctaa tttttcatta accggattgc agttggcttg tgtttcctct
     4741 tcaccgataa tttcattaaa aatctggcgt actgcttgtt gatattggct caatactgcc
     4801 ataaattgtt gccaatcgtt aaaggaatgc tccacccaat gcgttttttc gctatataga
     4861 tcttgctgat aatagctttg gcaagcaaat acaaggcggg cttgcgaggc ttcatcagtg
     4921 ggtagggttt gagtttgttt atcgtcaatc gcttgtaata cattttcaat ttgacggtaa
     4981 aaaatatagg cattgtggag cgtttccacc tgcgtttggg ttaataaatt taattctgcc
     5041 aagcgtggta gcacttttaa caggctacgt tcttgtaaaa ttttatctcg cccgccacgg
     5101 atcatttgga aagtttgggc gataaactcc acttcccgaa tcccaccggc accaaattta
     5161 atattatcgg ttaaattacg gcgaaccact tcacggctga ttttttcttt catttcacgc
     5221 aacgattgga ttgcactgaa atccagataa cggcggtaaa caaaagggcg gagcatttgc
     5281 tttaaatagc gatgatttaa attttggcta tcctcgccta aaatttttgc tttaatcatc
     5341 gcataacgtt cccaatcacg cccttgctct tggtaataat cttccattgc agtaaaactc
     5401 agcaccaagg ctccgctatc accaaagggg cgaagtcgca tatcggtacg atacacgaaa
     5461 ccatcagcgg tgatctcatc taatacttta attaaccgct gccccattcg ggtaaaaaat
     5521 ttgctgtttt caatcgattt gcgtccacct tcggtttcac caatatcggg gtaggtaaaa
     5581 atcaggtcaa tatcggaaga aaaattaagc tctcgtccgc ccaatttgcc catacctaaa
     5641 atcagcagtt cttgtgtttc gccaagagtg ttctttggcg tgccatattc ggcacagcaa
     5701 cgctgaaaca accaatctcg agcggctaga atcaggctct cggctaaatc cgacaaatgc
     5761 tgaaatacca attctaccgt cgctaattta tttgactgaa taaagctcag acgagccaat
     5821 tcacggtggc gaaattgccg caaaaccctc cccaacattt cttcattttc cactcttgct
     5881 aatatttttg ctaaacgctc agtataattg cagcaatcat caggcgtagg aaacttggca
     5941 agccattctg ccagtaaatc cggctgtttt tgcaaggttt gatgcacgaa attggaaagc
     6001 gaaatcgccg tcacaagcgg ttgaatttgc tcagaatttt gcaaattggg cggtaaattg
     6061 agttgggata agggaattag ggtggtaagc ataaatttag gctcaaaatt gaaaatagat
     6121 agaattttac gctcgatcaa ttcccctctt tagcaaagag ggcttagggg agatttggca
     6181 gaggtaattt tactctcata cgaaacatta atatcgtaat caaatctccc ccagcccctc
     6241 ttttctaaag aggggagaaa aaattaaaac actcttttaa acggtttaat aatcacatcg
     6301 ctatacacac cggcttccac ataaggatct tggcttgccc attgttgagc ttcttcaagt
     6361 gagttgaact ctgcaattac agttgaaccg gttacgcttg agccgtcttc agtcgggtta
     6421 ggccctgcga ttaataatcg accttctgcc tgtaattttt ccaaacgagc caaatgagcc
     6481 gggcgaactt ctaaacgttt ttccaacata ttgggattat cttgggcaaa gataacataa
     6541 tacatactat tctccttgtt tttcaggggt taaatcaatt agcgataatg cttcgtctaa
     6601 ttcggggtta ttttctctcg gcacagcacg cggtttgcct tctttatcaa tcgctacata
     6661 ggtaaataag gcttcggtta cgagataacg ttcgcgattg ccctcataaa cttgtttgat
     6721 aaagacctcg actttcactt gcatcgaaga acgccctatg tgcattaact tgccgtagca
     6781 gcagaccaca tcaccgactg aaatcgggcg gtggaaaatc attttatcta ccgttaccgt
     6841 aacgactcta ccttttgcca actctttggc taaaattgca ccgcctaaat ccatctgcga
     6901 cataatccaa ccgccgaaaa tatcgccatt ggcgttagta tctgacggca ttgccaaagt
     6961 tcttaaaatc agtttccctt ccggggcttt tccatagggt ttatctttca tttttttgtt
     7021 cctgcttttc agcatctttt aagtaagggt aaaggacgaa accggtaata accgttgcga
     7081 ctaaggttaa gccaataatg ccaaaggatt tgaaatcaac ccaaatctct tccgataaat
     7141 attgactgat ataaatattc agcagcatac aaatcagaaa gaatactgcc caaccgaagt
     7201 ttattttatt ccacactgct tcaggcaact gaatttcttt gcctaataat tttttgatta
     7261 atggtgtttt gaacacaaat tggctcacta atagcaccaa tgcaaacaga gcgtaaacaa
     7321 tggttacttt ccattgtaaa tatttaactt cattaaaata ggcactcagt aagccgaaaa
     7381 agaccaccga gccacccata ataatttgct gtttttcaat tttgccgtag agcattttta
     7441 aggcgatcat ttgcacgatt gtagcgataa ccaatgcgat agacgccatc tgaataccgc
     7501 tcattttata cacgacaaaa aacagaatca gcggaataaa ttcaagtaac tgtttcattg
     7561 atactcctaa cgcactgccg gcatttggcg gtaaacttgg taaaagcgga agctgaaaat
     7621 cgccataaag acgttaatta ccgcacttaa aacaatggta atgaatatgc caataaagtc
     7681 attaccaaac actgtgaaca aacgagataa catccccggt aataaataag aaatcacaca
     7741 aaataaaatc agcggtagca tttttccacg tgataattga agcgtaaatt taatactttc
     7801 cgatagactt ttctgcggtt tctccaataa ataagcataa atcactaacg ataatttgat
     7861 aaaaaaataa aaaccgacta ccaacaccgg caatgcaata atcactgttt caggcgatgc
     7921 cattgcaaaa gatgcaccga gagaaagcgg taacaccatc acaaattgta acaacagcac
     7981 aggtaggaag tgctttaatg cgtttcctgc attttggaaa aattgacgat aattgccatc
     8041 gttaatcgat tggatattga aaatcattaa gacattgatc aaaagattga caacacctaa
     8101 taaaatagtt ggcaatagta tagacaccat tttagaggca tcaatttgaa gttgttgcgg
     8161 ttgcgactcc gccattagct caggcgaatt tggcgacact aaaataaaaa ccaactgcac
     8221 aaccacaatg gtgacaatgg caaacagtga aaatgcttgt tgattacgca taaagttcca
     8281 actttgctgc aataaatcga caaatttaat tgacataaaa tacccttcaa tagattgtaa
     8341 atattttgtt gtgaagtata gca
//
LOCUS       DANLUA010000107         8307 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLUA010000107 DANLUA010000000
VERSION     DANLUA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..525
                     /gene="proQ"
                     /locus_tag="PXB44_001750"
     CDS             1..525
                     /gene="proQ"
                     /locus_tag="PXB44_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL5282207.1"
                     /translation="MEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPLKVGL
                     FQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVTEDEA
                     THAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLPKAS
                     EEKLAALADKFGRK"
     gene            611..2623
                     /gene="prc"
                     /locus_tag="PXB44_001751"
     CDS             611..2623
                     /gene="prc"
                     /locus_tag="PXB44_001751"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL5282208.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            2741..4246
                     /locus_tag="PXB44_001752"
     CDS             2741..4246
                     /locus_tag="PXB44_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL5282209.1"
                     /translation="MVAFSGMALAETATVAQVASEPVVQDNILPQLSFAAQQARAAEL
                     VKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWND
                     KAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVF
                     KNANNWLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRNHLYRDTAQRVLA
                     MAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLY
                     YFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPG
                     YADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSD
                     AIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTT
                     SAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
     gene            4416..4970
                     /locus_tag="PXB44_001753"
     CDS             4416..4970
                     /locus_tag="PXB44_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL5282210.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            4987..5616
                     /locus_tag="PXB44_001754"
     CDS             4987..5616
                     /locus_tag="PXB44_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5282211.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(5651..6022)
                     /locus_tag="PXB44_001755"
     CDS             complement(5651..6022)
                     /locus_tag="PXB44_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL5282212.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(6025..6384)
                     /locus_tag="PXB44_001756"
     CDS             complement(6025..6384)
                     /locus_tag="PXB44_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL5282213.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            complement(6397..6996)
                     /gene="rnhB"
                     /locus_tag="PXB44_001757"
     CDS             complement(6397..6996)
                     /gene="rnhB"
                     /locus_tag="PXB44_001757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL5282214.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            complement(6993..8030)
                     /gene="rsgA"
                     /locus_tag="PXB44_001758"
     CDS             complement(6993..8030)
                     /gene="rsgA"
                     /locus_tag="PXB44_001758"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL5282215.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
BASE COUNT         2562 a         1481 c         1800 g         2464 t
ORIGIN      
        1 attgagcaaa aagtagaaca aactcagaat aaaaccaatc caagtaccaa ggaagtgatt
       61 gcttatttag cggagaaatt tccgctttgt ttcacgcttg aaggtgaagt aaaaccattg
      121 aaagttgggt tgtttcagga attagctgaa gcattaaaag atgatgagaa aatcagtaaa
      181 acgttattgc gccaagtatt gcggaattac actaattctt ggcgttactt ggctgccagc
      241 caagctaatg ttgctcgtgt cggcttacag ggtgaagaag ttggcgtggt aacagaagac
      301 gaagctactc acgccgcaga agctttggct gctgcgaaag cggtggttgc agaacgtaaa
      361 gcacaagagc gtaaagcaca acgtaaagag tacttcaaac agaaagcacg tgaagaaaat
      421 gcgaaaaagc gtgcagaatt taaggcgaaa aaagcaatag ataatttacc taaagcttca
      481 gaagaaaaat tagctgcatt agcagataaa ttcggtcgta aatagttttc aagtcgcttt
      541 gtgcttacaa gcggtcggat tttagtaaaa ttttgcaaaa aaatgaccgc ttgattattt
      601 ggaaaagaag atgaaattaa caagattaaa taaaattttt gccctagccc taggtttaat
      661 gataggaaac gcttttgccg ttcagccgga cattaaagaa agtgcgattg tgatgccaaa
      721 acctaatgag cagcatagct tatcaaccaa gcgtgtaaca gcccgattaa ctcaatcgca
      781 ttatcataaa tttcaactga atgatgagtt tgcagggaag atttttaacc gctatgtcga
      841 ttggcttgat ccttctcata atacttttct tcaagccgat attgatgaaa tgcgtagtaa
      901 atatgctaca acattagatg atgatttata tgctggtaaa ttagatgcag catttgcaat
      961 gtatgagtta atgtctaaac gtcgttatga gcgttatagc tatgccttat ctttactaga
     1021 taaagagcct gacttaaaag ggaatgatga aattgagatt gaccgtgaga aggccgcatt
     1081 tccaaaaagt gtagaagagg caaatacact ttgggagcag cgagttaaaa acgatattat
     1141 caatttatat cttaaagata aaaaatggcc tgaaattaag aaaacactca ctaaacgtta
     1201 taactttgcg attaagcgtt taactcaagt taaggctgat gatattttac aaacttactt
     1261 aaattcattc tctcgtgaga tcgatcccca cactagctat ttgtctcctc gtgcggcaaa
     1321 agcgttccaa gagagcatga atctgtcgct agaaggaatt ggtgcaacgc taacccaaga
     1381 agatgatgta accagcatta gagcattagt gccaggagcc cctgccgcta aaagtaagaa
     1441 gctggtagtc ggcgataaaa ttgtcggtgt aggtcaaggt gagagaggtg agattgaaga
     1501 tgttatcggt tggcgtttgg ataatgtagt cgataaaatc aaaggtaaaa aaggcactaa
     1561 agttcgttta gaaattgagc ctgaaaaagg tggaaaaact aaaattatta cccttgtgcg
     1621 tgataaagtt cgtattgagg atagtgctgc taaattaacg gtagaaaaag tagatggaaa
     1681 aaatgtaggg gtaattaaga tccctacttt ttatatcggt ttgacttcag atgtacgtaa
     1741 attacttact caaatagaga aaaaaaatgt tgaaggattg atcattgatt tacgtgaaaa
     1801 cggtggtggt tcgttaactg aagtgattga attaactggt ttatttatta aagatgggcc
     1861 tgtagtgcaa gtacaagacg cattcaatcg tatcaaagta cacgaagatc cagaatcaaa
     1921 atcagtttat gatggaaaag tgatggtgat gattaatcgc catagtgctt cagcctctga
     1981 aatcttcgct gcggcaatgc aagattacaa ccgtgcaatt attgtagggc aacaaacctt
     2041 tggtaaggga actgttcaac agagccgttc attgaatttt gtatatgatt tggatcaaga
     2101 gcctcttggt tttatccaat atacgattca aaaattctac cgtatcaatg gtggaagtac
     2161 ccagctaaaa ggggttgagg cggatattaa attcccggaa atcattaatg ctgcaaaaac
     2221 aggtgaaagt tttgaagata atgcgcttcc ttgggataaa attccagcag ctaattataa
     2281 agaggctggg aatgctcgta acgcggttgc tgcattaaca gcgaaacatc aagagcgtat
     2341 tgctaaaaac ccagagttta tagtattaaa cgagaatatt gcgattcgaa aagaacgtga
     2401 tgagcgtaag tttttatcgt taaatttaga agaacgcctg aaagaaaata aagcagatga
     2461 aagcaagcgc ttaaaagata ttaatgctcg ttttgcccgt gaaggcaaaa aagcattaaa
     2521 agatattgat gatttaccaa aagactatga agcaccggac tttttcttaa aagaagctga
     2581 acagatgatg cttgattggt cagaatttga taaaaaatca taaatgtgtt tataatgcgc
     2641 acataacgta ccgcttagct ccgctaagcg gtttattttt ttataaattt gcaaatatta
     2701 agaggaagag tgggtgaaaa cctttaaatt attacctatt atggtggcgt tttctggcat
     2761 ggctttggct gaaacagcca cagttgctca ggtggcttct gagcctgttg tacaagataa
     2821 tattttacca caattaagtt ttgcagctca gcaggctcgt gctgcagaat tggtaaaaca
     2881 ggccgattat gcgttagaac aggaacaaat tcgtcaagag caagcaagaa aatttgcaga
     2941 agcggaagca agaattaagc aagaaatagg taataatgcc ttattgctga gccaagcaac
     3001 aactaaggtt tatttagaca gtgaatttgc gacaatgtgg aatgataaag ccgcagaaaa
     3061 agcatttttg aaagaatatg cattatttgc agtaagtggg gtatcctcta aatcagctaa
     3121 ggcattgcag caaatcttaa atcaaccgga aggtttaggt agagatattt tattaactga
     3181 tggcttctta gattatttgt attacaacaa aaatgtgttt aaaaatgcaa ataattggtt
     3241 atataactta ggcagttatg taccaaaatc gccctcttca gagcaaatct tccaatggac
     3301 taatgccgtg agggaaggcg attctgggca gtttgtaaca gaccttgttc caagaaacca
     3361 tttatatcgt gatacggcac agagagtatt ggcaatggca ggaggctctt cagcacctaa
     3421 agccaaaaaa ggtaaagctg ttacggaggt agctggtggc ggtaatgggg cttctttcta
     3481 taaattagcg ctcaatgctc aacgtttacg tattattcca agttttaaca atggaatttt
     3541 tgtgaatatc ccaagctatc aattatatta tttccgtgat ggtaatttag ctcttcaatc
     3601 taaagtgatt gttgggcgag aagatcgccg tacaccggta atgtatagta agctgagcaa
     3661 tgtggtagtg aatccacctt ggaatgtacc gactagtatt atgactaaag atattattcc
     3721 taaactagcg cgtgacccag gttatgcaga tcgtgctagt ttcgaaattc ttgatagtag
     3781 tggcaataaa attaatccgc gtagcgtaaa ttggtcacaa tatttaaatg ctaaaagttc
     3841 accataccgt attcgccaga aagcaggtga tgatagcgct ttaggacgtt ataaatttaa
     3901 tatgccaagt tcggatgcaa tttatttgca tgatacgcca aatagaggat tatttggtaa
     3961 aacggggcgt gcgttaagtt ctggttgtgt ccgtgtagaa aaatctgatg aattggcaac
     4021 cattttatta aaagaagcgg gttggggatc tgataaaaag aatcaagtct taggcagtaa
     4081 gaagacgacc tcggctcaga ttcgttcaga taacccggtt tatctctatt atgtgacggc
     4141 ttgggttgaa ggtggaaaag tgcaaaccct tccggacatt tataactttg atgccaaaat
     4201 cccacaacat tctgtgaatt ggggaaaatt aaagagcatt atttaatcgt gctatttctg
     4261 ataaattcca agaagatatc atcttcttgg aactttttga aaatatagct tacataaatt
     4321 tacgattgta aatttttact ttaagtatgg tgtatagaaa ttcgctcatt tgtgagtgat
     4381 tttctataaa tcattagcta acctatgagg aaaaaatgaa acaaattaat gttcaacgcc
     4441 gtaagtggct atcactcggc ggacttgttt taggggctag ccttctgccg ggtcaagtaa
     4501 tggcagcgct ttctactcct gccccagctg cgttacgttt tcgtaatgtg aatacaggcg
     4561 atacttacac tgctaaattt ggtgcaggtg gattaaataa aaccgattta agccagttaa
     4621 actatttaat gcgggatcgt catatcaatc aagttaaagc aattgatcca aaattatttg
     4681 tcaaattgaa tcaattacaa cgccgtttag gtttccataa tgcggaaatc ctagtattaa
     4741 gtggctatcg ctccgcacaa acaaatgcaa gacttcgtcg tagaagccgt ggtgttgcca
     4801 gccatagtta ccatattctt gggcaagcag tagatttcca agtatcaggt gtgccgttat
     4861 ataaagtaaa acaagcggct gagagcttga ataatggtgg cgtaggttat tatcctcgta
     4921 gtaactttgt tcacgtagat actggcccag ttcgcacttg gagaggctaa caagcggtct
     4981 tattttatgt ttaatttgca aattattcct gttagtccat ttcaacaaaa ttgcacggtg
     5041 atttgggacg aaaataagaa tgcggctatt attgatggcg gcgataatgc cgaaggcatt
     5101 attcaatttg ttgaatctca gcagcttaag gttcagaaat tattgattac gcacggtcat
     5161 ttagatcata ttatggcggt ggaaaagttg agagagcatt ttcaagtaga ggttttcggc
     5221 tcgcatcttg cagataaacc tttatttgaa cgtttgccgg aaatgtgcca acaatttgga
     5281 ttaccaccag taaatggctt tttgccggat cattggttgg aggaaggtga tcgggtcgaa
     5341 gtcggcgagc tgagctatac cgttcgacat ttgccgggtc atgctccggg tcatatcggt
     5401 ttttttgatt ttgacaatcg tattgctttt agtggcgatg ttttattcca aaatagcata
     5461 ggtcgaacag atctctattt aggcaaccat agccagttat tggaaacgat cagggaaaaa
     5521 atgtttgatt tagatgatga tttcatcatc ataccgggac atggtccaca cacaactatc
     5581 gggcgggaaa aacagaccaa cccgtttttg aaataaataa agaagcagag cagttggctc
     5641 tgcttttttg ctattttacg cctcgtaaat agaggcggta attatggctt tcttccgtcg
     5701 gtttaatgag tgcaatggtc tgtttttgct taggttgcaa ccatagatgt tgccagccga
     5761 tactatctga tataccgtta tgggattgtg ttaccccatc tttattatac caaaataatt
     5821 tataagtaag attaagcgga aattcagtta aatttgtggt gctaaattgc ttgctattcg
     5881 tttccagctc tatcttttca gcaatgtcag actcaatatt gacaataggc tctgagccgg
     5941 ttggtaaata gctgacaggt ttgctaccac aagcggtcaa aaaaatcgaa aaaattgcaa
     6001 atgcaaagta ggaaatacgc atagttattt tgccactaaa ggtcctagtt tttgtccgcc
     6061 taacaagtgc atatgaatat ggaacacttc ctgcccgccg tttttattac aattcatcac
     6121 taagcgatag ccttcttcgg caatgccttc ttcttgggct aattttgccg ccaccgtaaa
     6181 taaacgccct agggctaatt catcttcatt actcacgtga ttgacggttg gaataaattt
     6241 attcggaata atcaaaatat gcgtgggtgc ttgtggagca atatcacgaa atgcggtcac
     6301 aagttcatct tgatagacaa tatttgccgg aatttcttta ttgatgattt tggtgaaaat
     6361 cgttttttgg tactctgtag tcattgtgtt ttccttctaa tcgattaaac ctaatacttt
     6421 tcttacaggg gcaaaactct tacgatgata aggtgttgcc ccaaattctg ctaatttttc
     6481 aaaatggagt ttagtcgggt agcctttgtg ctgtgcgaag ccgtattgcg gaaattgttg
     6541 gtctaattcg tccatttctt gatctcgggc gactttggca ataatcgaag cagcactgat
     6601 ttcagccact aagctgtctc ctttcaccac cgcttgtgcc ggcattggca gttttggcgg
     6661 acgattgccg tcaattagca caaaatccgg ctgaatatgc aagccggcaa ccgcacgttg
     6721 cattgccaac attgtcgcat ttaaaatatt cagctcatca atttctgtcg gctcggcacg
     6781 tcctagcgac cagctcagag ccttggcttt aatctcttct gccaaagctt ggcgtttttt
     6841 ctcggataat tttttggaat cggttaagcc ctgaatcggg ttattcggat ctaaaatcac
     6901 ggctgcggtc acaaccgctc cgactaaagg tcctcgtccg acttcgtcaa caccggcgat
     6961 taatcgggcg ttagggtaaa taaattcatt cattatactt cttcttttct gaaatggcgt
     7021 tggcttttgg tttcagacag gctttcaatt aagcgatggt aattttcaaa ccgaattgca
     7081 gaaatttcgc cattttcgac ctcttgtctt aaggcacaac ccgggtcggt tttgtgtttg
     7141 caatcacgga atttgcacgt gcctaaaacc gattgaaatt ctcgatagcc taaggtgatt
     7201 tgctcaggtt ctaaatgcca taagccaaat tctcgaatgc ccggcgagtc aatcagctgt
     7261 ccgccttgcg gtaaatggta taagcgagag gcagtggtgg tgtgctgacc taaccctgag
     7321 ttatcgctca ctgagccagt gagagcattg acttcaggta acagttggtt aattaggctg
     7381 gatttcccca cgccggattg tccgacaaaa atagacgtac ctttagcaag atattggtgt
     7441 aacgcctgca tattttcacc cgtatcagcc gataaacata gggtttcata gccaatctct
     7501 tcatagattt tgagctgctc tttaactgct tgccgttcgc tttcgtttaa taaatcaatt
     7561 ttgtttagca cgattaaggc aggaatattc gccgtttcgc aaatcactaa ataacggtcg
     7621 ataatattca gcgaaagtgt aggtagcaca gctgatacaa taatgatttg atcgatgttc
     7681 gatgccatca ccttaatacc gtcataataa tcggggcggg taagttcatt tttacgtggg
     7741 aagacggctt caatgacccc actaatccct tgtagttgct cactaccttt acgccaagaa
     7801 acctgatcgc ccaccactac atttttcaat gtacggcgga tattacagcg gaaaatttgc
     7861 ccctctgcgg tttcaacatc agcgtgttta acgtggcgag taacgacgat gccctgttgc
     7921 atttctccta acatttcatc ttgccattca atctcttttt tgttgatacg cttatgatgg
     7981 ttagattgga ttctacgctg ttggttatgg gttaatttcg gtttactcaa aagaactctc
     8041 ggtaattatt gcaaatgaaa aataggcttt agcatactat aaaacctctt tttataatag
     8101 attaaagtga aagaataaaa aagcagaaag gctagaataa aggtagcgaa taatatagaa
     8161 tgcgatctag gttttgtgct gtttttttac atagaaaaaa accgcccaaa gtgggcggtt
     8221 ataaaccaat acggttcaaa atatacagga agtgaaacaa gtaactttcg ttactctagt
     8281 ctagtttcct agaccaatat gcaaaca
//
LOCUS       DANLUA010000108         8286 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLUA010000108 DANLUA010000000
VERSION     DANLUA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..411
                     /locus_tag="PXB44_001759"
     CDS             <1..411
                     /locus_tag="PXB44_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5282216.1"
                     /translation="SAAAIGSYPAIKEIGEERAKKHQDILDLNRELYSAMVNRDFSAL
                     QTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLSTDFQGNQSSGLLRVNGE
                     FFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            424..1317
                     /gene="murQ"
                     /locus_tag="PXB44_001760"
     CDS             424..1317
                     /gene="murQ"
                     /locus_tag="PXB44_001760"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL5282217.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(1401..2345)
                     /gene="prmB"
                     /locus_tag="PXB44_001761"
     CDS             complement(1401..2345)
                     /gene="prmB"
                     /locus_tag="PXB44_001761"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5282218.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            2438..2983
                     /gene="smrB"
                     /locus_tag="PXB44_001762"
     CDS             2438..2983
                     /gene="smrB"
                     /locus_tag="PXB44_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL5282219.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3047..3532
                     /locus_tag="PXB44_001763"
     CDS             3047..3532
                     /locus_tag="PXB44_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL5282220.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(3534..4283)
                     /locus_tag="PXB44_001764"
     CDS             complement(3534..4283)
                     /locus_tag="PXB44_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5282221.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            4455..5318
                     /locus_tag="PXB44_001765"
     CDS             4455..5318
                     /locus_tag="PXB44_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL5282222.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            5442..6035
                     /locus_tag="PXB44_001766"
     CDS             5442..6035
                     /locus_tag="PXB44_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282223.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
     gene            6390..6701
                     /locus_tag="PXB44_001767"
     CDS             6390..6701
                     /locus_tag="PXB44_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282224.1"
                     /translation="MVIIMLSKTKSPTLFPKYQNALLQLGENIRLASKRRMFTQTLLA
                     QRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQDFTDVGKDDELGRKLQDIELLR
                     K"
     gene            6701..7942
                     /locus_tag="PXB44_001768"
     CDS             6701..7942
                     /locus_tag="PXB44_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL5282225.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
BASE COUNT         2566 a         1565 c         1788 g         2367 t
ORIGIN      
        1 tctgcggcag cgattggaag ttatccggca ataaaagaga ttggcgagga gcgggcaaaa
       61 aaacatcaag atatattaga tttaaataga gaactttata gtgcaatggt gaaccgtgat
      121 ttttcggctt tgcaaacatt actaagcgat gattttgagc tttattcatc aacaggtgaa
      181 gtgattgata agcaagaatg gatcaaatta atccaaaaga gagtattgga atttgataag
      241 gttgatttgc tatctaccga ttttcagggg aatcaatcat ctggattatt aagagtaaat
      301 ggtgagtttt ttgggataga gcgggataat gtggtggtag aaatgaatat tcgaaccatt
      361 gtgagaaata cagaacgcca aattaaatgt attgtgatta aagaggttta agtgtcgcaa
      421 ctattgaatg ccttagggca gatgattacc gaacaacgta atccgaattc gatgaatatt
      481 gaccgccttt cagcgttaga tatagtccaa gtcatcaacc aagaagacaa acaagtagcg
      541 attgcggtgg agcagtgcct gcctcaaatt gcacaagcgg tggaaaaaat tgtgcaagcc
      601 tttgagaaag gtggacgatt ggtttatgtc ggtgccggca cgagcggaag attaggggtg
      661 cttgatgctt cggaatgtcc gcccacttat ggcgtaaaac ctgaaatggt ggttggatta
      721 attgccggtg gtgagcgggc attacgtttc ccgattgaag gggcggaaga taatcgtgag
      781 caaggcaaag tagatttaca agcggtcaat tttagcgaaa aagatgtatt ggtcgggatt
      841 gcagccagtg ggcgaacgcc ttatgtgtta ggggcgatgg aatatgctaa gcaacttggt
      901 gcgaccacgg tgtcgattgc cagtaaccca aattcggcaa tggcacaaat tgcggatatt
      961 gcgattgaaa ccgttgtcgg gccggaagtg ctaaccggtt ccagccgtat gaaatccggc
     1021 acagctcaaa aattggtatt gaatatgctc actactgcgt cgatgatttt aatcggtaaa
     1081 tgctaccaaa atttaatggt agatgtgcaa gcaactaatg aaaaactcaa agctagagca
     1141 attcggattg tgatgcaagc gacagaatgt gaacgagaag tggctgaaga tacactaaaa
     1201 ttagccgaaa ataacgctaa attggcaatt atgatgattt taggtaactt agataagcag
     1261 aaagccgaag atttattaag ccaaaatcaa gggcgattac aaaaagcgtt aaaatagaag
     1321 tatgtctgct ctccccttgt gggagagaga cagatcaatc attcattcag aatgattgat
     1381 cagagagagg ggaggcagat ttaagcaaat aaatggcgat gtttcactag ttcttctttg
     1441 gttaaactga acacccctaa gccaccgtgt tcaaattcca gccagttaaa cggcacactg
     1501 gggaattgtt cgattaaatg caccatactg ttgccgactt cgcagactag cacgccatca
     1561 tcatttaagt aatcagcggc ttgagcaaga atccgcttgg ttaaatctaa cccatctttg
     1621 cccgagccaa gagctaatgc cggctcgtga tggaactctt cgggcatatc gcctaagtct
     1681 tcttcatcaa cataaggcgg attagtcaca attaagtcgt atttatcttg cggtaaattg
     1741 ttaaacagat cggaacagat cgggaaaact cggtgagaca cctgatgacg ctcaatatta
     1801 atttgagcaa cgtctaaggc atcaagggaa agatcaaccg catcgacttc tgcttccgga
     1861 aaacgctcgg cacaggcaat cgcaatacaa ccgctgccgg tacacatatc taaaattcga
     1921 tgtggctcgt ttttgataat gcctttaaac ccttttttga tcagctcacc aatcggtgaa
     1981 cgtggcacga tcacacgctc atcgacataa tattcaccgc cacaaaacca agccgagttg
     2041 gtaaggtatg ctacaggttt gcgagagcct aaacgctcac agattaaatt gatgatacgt
     2101 tctttctcaa ctcgagttaa gcgagaggaa taaagtgttt ccggtacatc aaacggcaaa
     2161 taaagccctg ccaacactaa ttgttgtgcc tcgtcccacg cattatcgta gccgtgcccg
     2221 taataaagat cagaggcatt aaaatagcta tacccccaac gcatcatatc ttgaatagtg
     2281 actaaatcat tcacaatggg cagggcggaa atttcatcaa gaagttcggt attatattta
     2341 aacattggcg tgcctttttt cgtattgtta aaatggctgt gttataccac attttgcttt
     2401 gtgttagaat gtaaaaatga ataagaagga aaagataatg aacgatacgt tattagacga
     2461 tgaatttgca ttattcagag aggcagcaaa aggcacgaaa aagctgacac aaaatacctt
     2521 tgtgccgaaa gcggaacctc gcaagaaatt gcaagaattg cgagaattaa aagaaaaagc
     2581 tgatacagaa ttttatttct cggatgaata tgagcctttg ctgaaagaag aaaatgaaaa
     2641 agtgaaatat ctgcgagaag atgttgatcc ttatattctc aaacagttac ggcgaggcga
     2701 tttccaaccc gagctgtttt tagatctgca tggtttaact cgagaaaaag cgaagaaaga
     2761 gttggcagcg ttgattttag cctgtgagcg ggaaaaggta tattgtgcca gtgtgatgac
     2821 cggctttggc actcgcacgc tcaaagatca aattccacgc tggttggttc agcacccaaa
     2881 agtaatggcg ttgcaccagg ccccgaaaga gtggggcgga gatgcctcta ttctaatttt
     2941 ggttgagcag cctgaaaatc ctgaaaaaat gtttgggcgt taaaattcgg ctgaattgcc
     3001 aattttttat taaaaacgac cgcttgtgta tcattaagga gaaatgatgt cagaacaaac
     3061 tttccaccaa cttacagcat tattagattc gcatcgagct cgctatcgag tggtaggaca
     3121 tccttctgcc gggaaatcgg aagaagtggc aaaaattcga ggaacagaaa tcgggcaagg
     3181 ggcgaaagcc ttagtttgca aggtaaaagg caatggggta aagcaagctg tgcttgctat
     3241 attacctgct gattaccaag cggatttaac aaaaattgca gagcatcttg gtgggttaag
     3301 agcctcactt gccagcccgg ctgaagtggc agaattgacc gattgtgtat ttggggcaat
     3361 tccgccgttt agctttcacc ccgatttatt attggtggct gagccaagtc tgtttgagcg
     3421 ttatgctgaa ttagccttta atgccggtac gttggaacgt tcgattattc tgaataccga
     3481 ggattaccaa aagatagtta atcctgtgtt ggttcaattc gccaaaagct aggttatggg
     3541 gcgaaaaact ccacgatttt gccaatagcg atatccatta atttagggtt agaaaaagcg
     3601 tgatctgccc cttcaatcgg gagcagttca atcacgttat tatcggcaaa ctgctgtgaa
     3661 gtgcttaacg ccaccatttc atcttccgtg ccgtgcagaa ttaaaatttg ctcggcataa
     3721 tccaaataat cgttttgggt aatatcggtt agctcatcaa aaaaggcttg gctgattttc
     3781 atctggcgat caaaccccca caaaatttct cgccctttgg caagtttttg ccgttcctct
     3841 tctgacaagc ggttcattaa cgaatggtag atttgtactg ccggtgcccg caatgcaatt
     3901 ttccgaaacg gattgccttt ttccgccaga tatttcaagg ttaaatagcc gccaaaacta
     3961 gtgccgtagg catacagatt tttagcattc aggctggttt tggcataatc aatcaccagt
     4021 tgcagataag taatggattc ctcaaccgag agttttttac gggcatcatc accgtggcaa
     4081 gggaaatcaa aggcaagggc agcgtagcct ttatattttg agctaaggcg ttccccaaat
     4141 ttggtattcg atttcagatc tttagacgaa ccaaagccgt gcaacacaat cactacattc
     4201 tcgatctgct gcaattcttt ttcataaaac agtttacagc gaatgctgag accctgttca
     4261 ttaatctcaa agcgtttttc cattttatgt taagtattga aattgaagat gacaaaattt
     4321 tagcactatt tgagtgcaag cggtcggatc tctcgcaaaa tttgcaaatt ttccggtaaa
     4381 tttaaccgct tgtactagcc aaaatttaag catatttata gaatacagcc ttcgttttta
     4441 aaaggagaaa tgaaatgtta caacttattt tagcgatttt atgtagcgtg actgtaggcg
     4501 tgttgattaa attcgcccgc caaaaaggga ttcgtatcga ccaaagtatt gcggtgaact
     4561 atatcgtgac taccacgatg acgtttttat tactaaaacc ggattaccaa gggcagtcga
     4621 ttaccgatat tattgctaat aacccgactt cctatatttt tgtgatttta agtatcacat
     4681 tgcctactat tttcttggtg caagcaaaag ccctagagtt tgccggtatt atccgaaccg
     4741 atgcggcaca acgtttatcg ctgttcctgc cgattttggc ggcattcacc atttttggtg
     4801 agcaggttac gcaaaataaa ttaattgcac ttctgttagc tttcgtggca ctcggttgct
     4861 tattatggaa aggcaatcag ggaatggaaa aaggcggaaa aattgcgatt atcagccttg
     4921 ctctggtttg ggtaggctat ggcgtgaacg atattttatt taaacagatt gccaaatcag
     4981 gctctgcttt ccctgtcacc ctttcgattg cctttatcgg tgcaggctgt tttatgttta
     5041 tctatttgct acttaaacgc actcaatggc acgttccaag tgtcttaacc ggcttattgc
     5101 taggcgtgtt gaacttcggc aatattctgt tctatattca agctcaccaa gccatgaaag
     5161 atgacccaac tttagtcttc actggaatga acttaggggt aatttgctta ggcactttcg
     5221 ttggagcatt cttctttaaa gagaaaatcc acaaagtgaa ctacttaggc gtggcgattg
     5281 cgattgtggc gattatttgc ttgttttatt ggcggtaaat atactttcca aaattaaggg
     5341 cgaacataat gttcgccctt aatttttgag taatatagtg taaaatattc aataaattcg
     5401 accgcttgtt gtaccgattc atatttcata aaggacaaaa aatgaagcta aaagccttat
     5461 ctacactcat tgcctcttca atgctatata gtcagagttt tgctgtaccg ccggaaaaaa
     5521 cagctgaaga gccacagccc tttttcaata aaaccgaatc tgggtatgat ttagtcttta
     5581 atgatcaaaa ctatcgttta attgaaacgg aattaaatgg cgagaaaatc caattccgtg
     5641 cctttgagcg gattgtgtat gtgaacaatc cgattgagcc agattaccaa acaattaatc
     5701 tgtatgtgcc ggaagcttac tacaataatg gcgaaatcaa tggctaccaa gcggataccg
     5761 ccccgatctt cttgccaaat tcagtggggg gctatatgcc ggcaaaagcg gcaagttatg
     5821 ataaaaaaag acgtgacggt tcaccaggta cgattttggt ggcgttatct aaaggctatg
     5881 tggtggcaag tgtgggagca agaggacgca cgctacaaaa agagggaaaa tacaccggca
     5941 aagcaccttc tgccattatt gattcaaaat ccgccgccca taaagcgtgt cggttcgagt
     6001 ccgaccactg gcaccactca ctatgtaaaa tttagtttta catagtttgc tgtaagcatt
     6061 tgaattttga atcgtttgtg taggattgtt caaatcatat tcgcaaatac acagttacta
     6121 cgatgtatcc agccaataca tccattggga ttttccgtcc gaatttcaga cgttctagat
     6181 cttacacatt ctttcaagtt tgtaaagcct gaaaatcatt ttttagattg attttatttg
     6241 aaaaaatcta ttttttcttt taattcaaat ttagtcaaaa cttcaccaga tatttttttc
     6301 cgaaaaaccc tctctaccta ttttactgtt tacataactg ctcttgcaat tgctataaat
     6361 ggacattata atttccaaaa aacataaaaa tggtaataat aatgctcagt aaaacaaaat
     6421 caccaactct gtttcctaag taccaaaatg ccttgcttca acttggagaa aatattaggc
     6481 tagcctcaaa aaggagaatg tttactcaaa cattattggc tcaaagaact gggcttagcc
     6541 gtgtaacagt gagaaaaatt atgaatggtg atccaactgt tgcgattgga tactacctaa
     6601 tggttttagg tgttctcggt ctagaacaag atttcactga tgttggaaag gatgatgagt
     6661 taggacgtaa attgcaagat attgaattat taaggaaata atgatgaata aacgaattta
     6721 tgtgtttgca gattgggaaa ctttttcaca gcctgaatta gttggggtgc tttcttctga
     6781 tgtagtgaga ggcaaagaaa atttccgttt tagctacgat aacgagtggt taagttcacc
     6841 ctatgtacag cagattgatc ctaacttata cctttaccct ggagagcaat acagtacaga
     6901 tacacaaaat tttcatatct ttttagattc ttgccctgat agatggggta gattgttaat
     6961 gaaacgtcgg gaggcagctc tagctagtca agagaatcgt aaacctaagt tactaataga
     7021 aagtgattac cttttaggcg tacacgatat gaatagaatg ggggctttac gttttaaaac
     7081 tgaattgaat ggtccatttt tggacaataa tactcgtctt gcagcaccac cactgtcttc
     7141 cttaagagaa ttagaatttg cagcaataca acttgaaagc agtgataatg tggacgatcc
     7201 ggattattta aaatggcttc atatgttgat atcaccaggg tcgtcactag gcggagctag
     7261 accaaaagct tcggtaatag atgaactaaa tcaactatgg attgccaaat ttccaagtcg
     7321 gtacgatgat tatgatattg ctctttggga atatttagct tatcgactgg ctattcaagc
     7381 caaaattgaa atggctactt gtagaattga acgctttaac caagaacacc atactttttt
     7441 gacacaaaga tttgatcgac attgtggaaa acgtcgccat tttacgtcag cattaaccca
     7501 gctgggttat tatgacggag actatgaggc aagctacctt gagattgctc aatttttaac
     7561 agaaagtggt tcaaatactc gaattgactt ggctcaacta tggagaagaa ttgtattcaa
     7621 tattgctatt tcgaatactg atgatcattt gcgtaaccac ggatttatct acgctcaaga
     7681 ggggtggata ttatctcccg catacgattt aaacccagta ccactatctc aaggattaca
     7741 tttaaacata acagacagcg ataaccgatt ggattatcag ctcgcctttg atgttgctga
     7801 cttttttcaa ttatctaagc aacaagccgt acagatttat gatgaagtac tgaaagaaat
     7861 taataattgg caaaatgtcg caaaagaaat tggtattcca cgccaagagc aagagcagat
     7921 gaaatttgca tttaatgtat agtaattgac ttgtggaacc aatcatcgta gtctatgttc
     7981 taaaaaagta gcgttacctt aataacgcaa tgagttgttg gctcattgtc aatctgatat
     8041 taatctagga tgtaaaggcc tagcggtggc aggcaccaga aagacctgat actaatctag
     8101 ggtataaaga cctagcggtg gcagacacca gaacaaacca acatcttata aaaccattag
     8161 taacacttga cttttttctc aaccatatcc gcaaggaagc gtagccttaa aaacgcaaat
     8221 gtaggaaact aatctaggca tatataatgc ctagcagatg gcagacatca gaaaaactaa
     8281 cattta
//
LOCUS       DANLUA010000109         8237 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLUA010000109 DANLUA010000000
VERSION     DANLUA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..910
                     /locus_tag="PXB44_001769"
     CDS             <1..910
                     /locus_tag="PXB44_001769"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL5282226.1"
                     /translation="IIEVQGPILSEVGNGMQDNLSGIEKAVKVNVKCIPSATYEVVHS
                     LAKWKRHTLARFGFKEGEGLFVHMKALRPDEDSLDRTHSVYVDQWDWEKVIPEGRRNF
                     DFLKETVNEIYKAIRLTELAVEARFDIPSVLPKQITFVHSEDLVQRYPNMTSKERENA
                     ICKECGAVFLIGIGGKLSDGKPHDGRAPDYDDWTTESEQGYKGLNGDILVWNEELGTA
                     FELSSMGIRVDETALRLQVALTGDEDRLEMDWHKDLLAGRLPLSIGGGIGQSRLVMFL
                     LRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1034..1453)
                     /gene="atpC"
                     /locus_tag="PXB44_001770"
     CDS             complement(1034..1453)
                     /gene="atpC"
                     /locus_tag="PXB44_001770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL5282227.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1481..2854)
                     /gene="atpD"
                     /locus_tag="PXB44_001771"
     CDS             complement(1481..2854)
                     /gene="atpD"
                     /locus_tag="PXB44_001771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL5282228.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(2875..3741)
                     /gene="atpG"
                     /locus_tag="PXB44_001772"
     CDS             complement(2875..3741)
                     /gene="atpG"
                     /locus_tag="PXB44_001772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL5282229.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3767..5308)
                     /gene="atpA"
                     /locus_tag="PXB44_001773"
     CDS             complement(3767..5308)
                     /gene="atpA"
                     /locus_tag="PXB44_001773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL5282230.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5321..5854)
                     /gene="atpH"
                     /locus_tag="PXB44_001774"
     CDS             complement(5321..5854)
                     /gene="atpH"
                     /locus_tag="PXB44_001774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL5282231.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(5868..6338)
                     /gene="atpF"
                     /locus_tag="PXB44_001775"
     CDS             complement(5868..6338)
                     /gene="atpF"
                     /locus_tag="PXB44_001775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL5282232.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6399..6653)
                     /gene="atpE"
                     /locus_tag="PXB44_001776"
     CDS             complement(6399..6653)
                     /gene="atpE"
                     /locus_tag="PXB44_001776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL5282233.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6771..7559)
                     /gene="atpB"
                     /locus_tag="PXB44_001777"
     CDS             complement(6771..7559)
                     /gene="atpB"
                     /locus_tag="PXB44_001777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL5282234.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7582..7965)
                     /locus_tag="PXB44_001778"
     CDS             complement(7582..7965)
                     /locus_tag="PXB44_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL5282235.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         2444 a         1852 c         1538 g         2403 t
ORIGIN      
        1 cattattgaa gttcaggggc ctattttgag cgaagtcggc aacggtatgc aagacaacct
       61 ttccggtatc gaaaaagcag taaaagtaaa cgtaaaatgt attccaagcg ctacttatga
      121 agtggttcat tctcttgcaa aatggaaacg ccacacctta gctcgttttg gatttaaaga
      181 aggagaaggg ttatttgttc atatgaaagc tcttcgccct gatgaagatt ctttagatcg
      241 cacacactcg gtatatgttg accaatggga ctgggaaaaa gtcattcctg aaggtcgccg
      301 caacttcgat ttcttaaaag aaacggtaaa cgaaatctat aaagcaattc gtttaaccga
      361 attagcggtg gaggctcgtt tcgatattcc atctgtattg cctaaacaaa ttactttcgt
      421 tcacagcgaa gacttagtgc aacgctatcc gaatatgacc agcaaagagc gtgaaaatgc
      481 tatctgtaaa gagtgcggag cggtgttctt aatcggtatc ggcggcaaat tatcagacgg
      541 taaacctcac gatggacgag ctccggacta tgatgactgg acaaccgagt ccgaacaagg
      601 ctacaaaggc ttaaacggcg atattttagt ttggaacgaa gaactcggca cagcctttga
      661 actttcctca atgggtattc gtgtagatga aaccgcctta cgcttacaag ttgctttaac
      721 tggcgacgaa gaccgcctcg aaatggattg gcacaaagat ttattagccg gcagactccc
      781 gctttctatc ggcggcggta tcggtcaatc tcgcttagtg atgttcttac tacgcaaaaa
      841 acacatcggt gaggtgcaat ccagtgtctg gccaaaagcg gtatttgaag agtttgagaa
      901 tattctttaa taaaacaaaa aagcgaacct tagggttcgc ttttgctttc tacaagcggt
      961 cttattttgc aaaaaattta ctgtttttaa ccgcttgtaa aacaaaaccc cactttcgtg
     1021 gggttttttg atattaacgt ttattcgcta atttggtttt agtgagttcg taaacacgca
     1081 gtttcgcaat ttctctcgct aacttcgcgg tcatatccgc atcgctggtt tttgaaagtg
     1141 tttcttctac tttgcgtttt gcggcaagaa tacgctgttc gtctagttct tcaccacgaa
     1201 ttgcggtatc agccaacact gttactacgg tcggttgtac ttcaaggaaa ccacctgaaa
     1261 cgtagataaa ctcttctttg ccgtcagcta aagtgaattt aaccattccc ggtttaattg
     1321 cggttaataa cggtgcatgc cctgcataca cccctaattc accatctgtc cctgaaacac
     1381 gcacgctggt aactgtgcct tcaaagattt tacgttctgc actcactacc gtgagttcaa
     1441 attgagatgc catgttgttc tcctttagtt tagaacaaaa ttacatctgt tttgctcttt
     1501 caaccacttc gtcaattgaa cccgccatat agaacgcttg ttccggaata tggtcgtatt
     1561 cgccttctaa aatgccttta aagccacgaa ttgtttcttt taacggtacg aatttgcccg
     1621 gtacgccgtt gaatacttcc gcaacgtgga atggttgtga aaggaaacgt tcgattttac
     1681 gcgcacgagc aacaaggcgt ttgtcgtctt ccgataattc atccataccc aaaattgcaa
     1741 tgatgtcttt taactcttta taacgttgta atgtaccttg tacaccacga gcaacgttat
     1801 agtgttcctc accaaccacg agtggatcta actgacgaga ggttgagtca agagggtcaa
     1861 ccgccgggta aataccgagt gaagcgatat tacggcttaa taccaccgtt gagtctaagt
     1921 gggcaaaggt agttgccgga gacgggtcag ttaagtcatc cgccggaaca tataccgctt
     1981 gcacagaggt aatagagccg gttttggttg aggtaatacg ctcctgtaat acgcccatct
     2041 cttctgcaag agtcggctga taacctaccg cagacggcat acgacctaat aacgcagata
     2101 cttcggtacc agctaaagta taacggtaga tgttatccac gaagaataat acgtcacgac
     2161 cttcatcacg gaatttttct gccatggtta agccggttaa tgccacacgc agacggttgc
     2221 ccggtggttc gttcatttga ccgtaaacca gcgatacttt gtctaatacg ttagagtctt
     2281 tcatttcgtg gtagaagtca ttaccctcac gagtacgctc acctacaccg gcgaataccg
     2341 agtaaccgct atgttcgata gcgatgttac ggattaactc catcatattt acggttttac
     2401 ctacacccgc accaccgaat aagccaactt taccaccctt agcaaatgga gcaactaagt
     2461 cgataacttt gataccggtc tctaaaagct cggtgctgtt cgcttgttct tcgtagcttg
     2521 gtgctgcacg gtggattgtc caacgttctt cttcaccgat tgggcctgct tcatcaattg
     2581 gctcacctaa tacgttcata atacgaccga gtgtttttgt accaaccggt acttcaatcg
     2641 ctttattcgt attttcaact tttaagccac gttttaaacc atcagatgaa ccaagtgcga
     2701 tacaacggac cacaccgcca cctaattgtt gttgaacttc aagggttaat cctgattcaa
     2761 cttttaatgc atcataaact tttggtactg catcttgcgg aaactcaaca tcgattaccg
     2821 caccgatgat ctgtacaatt tttcccgttg ccattaccgt tcctctattc attgttaaat
     2881 tgctgcggca cccgcgacaa tttcatttaa ttcattcgta atacttgctt gacgagcttt
     2941 gttatacact aactgtaact cattaatcag attacctgcg ttatcggttg ctgctttcat
     3001 tgccaccatt cgagcagcct gttcagacgc aagattatct accactgcct gataaacttg
     3061 agattctaaa taacgaacca gtaagctgtc taataacgct tgtggatttg gttcatagag
     3121 ataatcccaa gaaccagttt gctctaatga gtcattttcc agtgcaggca aaggaagcaa
     3181 ctgttgtact gtcggttttt gtgacatcgt gtttataaaa cggttatata ccacatacac
     3241 ttcatcaatt tcgccttcac ggtaggcatt taccatacca ttaacaatac cgactaaatc
     3301 ttccattgcc ggtgtatcac ccaatccatt gatatgacct ttaatctcta gccccattgg
     3361 gttaaaaaag gagatccctt tatttcccac cacagcaagt cgagacttaa caccttggtc
     3421 atctttttct ttcaattcat gtaaaacggt tttaaataaa ttgatattta agccaccaca
     3481 taaaccgcga tcggttgaaa ctactaaata gccaactttt tttaccggac gtggggtcaa
     3541 aaatgggtgt ttatactcaa tgttaccttt ggcaatatgg ctaattgcct tgcgaatact
     3601 ttcagcataa gggcgagaag ccgccatacg ctcttgggtt ttacgcattt ttgatgcggc
     3661 aaccatttcc atcgctttgg taattttttg tgtattacga acacttgcaa ttttggttct
     3721 tatctcttta gcacctgcca tattatttct ccgctaaaaa gttagattac caagcactgt
     3781 tctttttgaa accagataaa atctcgttca atttatcttt aatttcatcg ttgaaattgc
     3841 cggattttga cagttcttgc ataaactcgc cgtagttact ttgagcataa gctaataagt
     3901 ttgcttcaaa tgagccaaca cgctcaaccg gaatatcatc taagtaaccg tattcagctg
     3961 ctgctaatga taaacctaat tgtgcaacag acatcggctc gaattgtttt tgtttcaaca
     4021 attcagttac tttttgaccg tgtgaaagct gtttacgtgt tgcttcatca agatctgatg
     4081 cgaactgggc aaatgccgct aattcacggt attgagcaag tgcggtacgg ataccaccgg
     4141 caagcttctt aaccagttta gtttgtgcag aaccacctac acgggaaacc gagatacccg
     4201 ggtttaccgc agggcgaata ccggcattga ataagcctga ttctaagaaa atctgaccat
     4261 cggtaattga aattacgtta gtcggtacga aggctgaaac gtcacctgct tgggtttcga
     4321 tgatcggtaa tgcggttaaa gaaccggttt gacctttcac agcaccgtta gtgaaacgtt
     4381 ctacatagtc tgcatttaca cgagacgcac gctctaataa gcgagagtgt agatagaaca
     4441 catcacccgg gaatgcttca cgacccggtg gacggcgtaa taacaatgaa atttgacggt
     4501 aagcaactgc ttgcttagac aaatcatcgt aaacgatcaa cgcatcttca ccacggtcac
     4561 ggaagtattc acccattgca caacctgcat aaggtgcaag atattgtaat gcagcagatt
     4621 cagacgcaga agccactaca acgatagtgt tcgctaatgc accgtgttct tctaatttac
     4681 gcaccacgtt ggcgatagtt gatgcctttt gaccgattgc aacgtagata catttaatgc
     4741 ctgaatcacg ttggttgatg atcgcatcaa tcgctaatgc ggttttaccg gtttgacggt
     4801 cacctataat caattcacgc tgaccacgac cgattggtac cattgagtcc accgctttgt
     4861 aaccggtttg aaccggttga tcaaccgatt gacggtcgat aacgcccggt gcgatcactt
     4921 cgattggtga gaaaccgtca ttttggattt cgcctttacc atcgattggc tgacctaagg
     4981 tatttacaac acgacctaat aagccacgac caaccggcac ttctaaaata cgaccggtac
     5041 attgtacttg cataccttct gctaaatcgg cataaggtcc cattacaact gcaccaacag
     5101 aatctctttc taagtttaat gcaatcgcat aacgattgcc cggtaatgca atcatttcac
     5161 cttgcattac atcagataag ccgtggatac gaataacccc gtcacttacc gaaacgattg
     5221 tccctgtgct ttgagcttca ctcaccacat taaactgggc aatacgtttt ttaatcaatt
     5281 cactaatttc agttgaattt agttgcattt tttattcctc ttacaagctc aactcttgac
     5341 tcaagcggtt tagctgtcca cgactactac catcaatcac tacatcatca taacgaatga
     5401 tcacacccgc aattaatgaa ctatctattg atgaagtaat acgtactttc gttccgagtc
     5461 gcttttccat cgcattggca atttttgctt cttgtgcatc tgttaattta tctgcagaaa
     5521 caacctcaac atctttaatt gattcctgtt ctgctcttaa ttctaaaaat gcattcaata
     5581 ccgcaggtaa tactgctaaa cgcttgttat cagccattac gcgaatgaaa ttttgcccat
     5641 attgatctaa ttgttcgcca caaatcttga taaacgtctc agaaatctga ccgcttgcaa
     5701 gtgatgagtt aagaaaacta gctacttgct catcgttagc gacggcggca gaaaaagtta
     5761 acatctgctg ccattgttcc aattgacctt gttctaaagc aaaatcgaaa gccgctttag
     5821 cataggggcg agctactgtg cttaattctg acatctgccc ctcctagtta tagttccgca
     5881 actagcttat caataatatc gttgttcgca gcggcatcca ccgaacgacc cacgattttt
     5941 tctgcaccag caatagccaa tgcagccact ttttgacgaa gctcttcttg aacgcgttta
     6001 cgttcgcttt caacttccgc gtaaccctgt tcaataatac gttgacgttc cacttcagct
     6061 tctgcttgta cagattctaa aatttcatta cgacgttttg ttgctaaatc aatgatattt
     6121 tgtgcttctt ctttcgctct aaggatttct tgatccgcag ccgctttaga atcagcttgt
     6181 tcttgtttcg ctttttcggc agaagccaat gcgctagcaa tatttgcctg acgctcttct
     6241 atcactcgaa ttagcggtgg ccatacaaac ttcatacaga acgcaacaaa aagtgcgaac
     6301 gcaatcagtt gaccaattag tgttgcattt aaattcacaa cgccctccta aaagccggta
     6361 aagttattca actgaataac ggttaatcaa atcccgactt atttcaataa atcaatgaac
     6421 gggtttgcga agatgaatag taatgaaata cctacagcga tcattgcgat agcatctaaa
     6481 agaccagcaa cgataaacat tttagtttgt aagcttgatg ctaattccgg ctgacgtgca
     6541 gatgactcta agaatttgcc acctaagata gcaaagccga tagcagtacc aagagcagcg
     6601 aaagctaata aaattgaagc accaataatt gttgctgtga ttactgattc cataatgttc
     6661 tccattagat tgaggaatta gcccgaaggc tgggttgttg ataaaaatac atatcaagcg
     6721 gtcaatattt tgcaaaaatt tgcgaaaaac ctaccgcttg taaggctagt ttaatgatcc
     6781 gctttgttat aagcgatact caaataaaca atcgttagca tcataaaaat aaaggcttga
     6841 agcgtaataa ctaaaatatg gaaaatcgcc caaaccaagt gtaatggaat ccccaacgcc
     6901 tgtagagcga agttgtctgc catatacatt actgcaataa ggataaagat taactcacct
     6961 gcatacatat taccgaataa acggaacgcc aatgaaatcg gttttgccaa aagcgttacc
     7021 gtttcaagaa taaagtttac cggaatgaat gcccagtggt taaacgggtg taacgtgtat
     7081 tctttcacta aaccgccaaa acctttcgat ttcaccgtgt aaaataaaat taacgcaaat
     7141 acacaaatag acatacccag tgttgcacta atatctgccg ttggtactgc acgcaaatag
     7201 tgaataccaa acagattggc taactgtggt aagaaatcga ccgggataag gtcgatcgca
     7261 ttcataataa acacccaaca gaaaatcgtt aatgctaatg gtgcaattac attgcgaggt
     7321 ccgtggaagt tatccttcac taaaccgttt acccactcaa taacaatctc aacaaaacat
     7381 tgcaattttc ccggaacgcc cgttgttgca ttttttgcta cacgcgagaa cacccataaa
     7441 aaaatcgcac ctgccactaa agagaagaac agtgtatcta aatgcactgc ccaaaagcct
     7501 tcacctgtcg ataaaaaact caagtgatgg ctaatatatt ctgctgtcgt tccagccata
     7561 acatatcctt ttaaattaaa attagtggtg atgtttgctt aatacaaacg ggatcatatt
     7621 atttaaaaat aatgcgataa aataacccac gaaaaacaac acaacatcta gagcagggat
     7681 aagtttaaaa cagccgatca ccaaaataat agtaaccaac catttcattc cttcaccacg
     7741 atagaaagcg gtcatttttg actgattttt tgcagatttc ttaaagaaaa cccaatatac
     7801 aaacacaaga tgaggcaaaa aactcgctaa agcaccgcct agaaaggaaa aattaaaatt
     7861 cattttccat aaagacactc caagtgcaaa aacgcacaag agaaaaaatt caacctttaa
     7921 tgcttgttta tattgtgatt ttgctctatt gattacagct gacacatttt atcctttttg
     7981 aataagtaaa ccacacaatc aggctaataa ttagccactg ttgaatcaaa gaaaatccgt
     8041 gctaaattat actttacaaa tgaattgttg caaccgaaaa gcgacacaaa aacttgttat
     8101 tcttcacatt tttgaaaaaa aattttattt acgttgcatt tttttgagta aaaccatcaa
     8161 aatcagttaa caaaaaaaca acaaatagag gtgtgtgtat ttttctctgt cacattattt
     8221 caatatttga taaattc
//
LOCUS       DANLUA010000110         8208 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLUA010000110 DANLUA010000000
VERSION     DANLUA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            166..597
                     /locus_tag="PXB44_001779"
     CDS             166..597
                     /locus_tag="PXB44_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5282236.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            615..1607
                     /locus_tag="PXB44_001780"
     CDS             615..1607
                     /locus_tag="PXB44_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282237.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1604..2593
                     /gene="oppF"
                     /locus_tag="PXB44_001781"
     CDS             1604..2593
                     /gene="oppF"
                     /locus_tag="PXB44_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL5282238.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(2697..3455)
                     /locus_tag="PXB44_001782"
                     /pseudo
     CDS             complement(2697..3455)
                     /locus_tag="PXB44_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3585..4226)
                     /gene="bioD"
                     /locus_tag="PXB44_001783"
     CDS             complement(3585..4226)
                     /gene="bioD"
                     /locus_tag="PXB44_001783"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL5282239.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4244..4999)
                     /gene="bioC"
                     /locus_tag="PXB44_001784"
     CDS             complement(4244..4999)
                     /gene="bioC"
                     /locus_tag="PXB44_001784"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL5282240.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5003..5650)
                     /locus_tag="PXB44_001785"
     CDS             complement(5003..5650)
                     /locus_tag="PXB44_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL5282241.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(5647..6810)
                     /gene="bioF"
                     /locus_tag="PXB44_001786"
     CDS             complement(5647..6810)
                     /gene="bioF"
                     /locus_tag="PXB44_001786"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL5282242.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(6807..8108)
                     /locus_tag="PXB44_001787"
     CDS             complement(6807..8108)
                     /locus_tag="PXB44_001787"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL5282243.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
BASE COUNT         2346 a         1851 c         1703 g         2308 t
ORIGIN      
        1 agcattctga aagggaacgc cacttttgtg ggaatgccac ctaaaccctc taccatctca
       61 gtgaagcatc attgagattt cattttactt ccacctcgcg gggaacattt ttcccgtgtt
      121 gggcaaggat gtttctccgc ttttttacat ttggagaaac aaactatgac aactcaaact
      181 tctactcaaa aaaattactt caaccttcat acaacaggca ttggctatct aaacgatatt
      241 cgtttggtag agcctaaaaa aggaaatcct ttttatgcct gccgtatagc agctctggtt
      301 ggcgaatctg actctcctga gtacagatat tttgatatga atgtagtcgg taaagagact
      361 gaaacactta tcaaaagatg ccaaaaagca gttagtgaag agaaaaaagt gcttgtgtca
      421 tttgtaatgg ctgatttgtg gttcgataca tttacttact caacagatag taaattccac
      481 aaaaaaggcg ataccggtgt atctgtaaaa ggtcgcttaa tccgcatcaa aatgattaaa
      541 attgatgggg aaatagccta ccaagaaaag ccaaaatcag atgagcaatc tgagtaattt
      601 tacctaagcc cgacatttta tgttgggctt tattactcga tgtacaagat ttgcgcgtaa
      661 cttttaagac cccagatggc gatgttactg ccgtcaatga tctgactttt tctttaaatg
      721 ccggtgaaac cctcggcatt gtcggcgaat ctggctcagg aaaatcgcaa accgcttttg
      781 ctctaatggg gctattagca gataacgggc gaatttcagg atcagcaaaa tttaatggac
      841 aagaaattct caacttgcct gaaacacaac ttaaccgctt gcgtgctgaa caaattgcga
      901 tgatcttcca agatccaatg acctcgctga acccttatat gaaaatcggc gaacagctaa
      961 tggaagtact gatgttgcat aaaggcatgg atcataaaac tgcgtttgaa gaatcggtac
     1021 ggatgttaga tgcggtgaaa atgccggagt cacgtaaacg tatgaatatg tatccgcatg
     1081 aattttccgg cggtatgcgt cagcgtgtga tgattgcgat ggcattactt tgccgtccga
     1141 aactcctcat tgcggatgaa cccacaactg cgctcgatgt gacggtgcaa gctcaaatca
     1201 tgaccttatt aaacgaatta aaaagcgagt ttaataccgc cattatcatg attactcacg
     1261 atctcggtgt ggtcgcagga atttgcgata atgtgttggt gatgtatgta ggcagaacca
     1321 tggaatacgg caccgctgag caaattttct ataccccaag ccacccttat tctatcggct
     1381 taattggtgc agtgcctcgc gttgacggac aggaagaaga actcattact attccgggta
     1441 atccacctaa cttattacgc ttaccgagtg gctgtccttt ccaaccacgt tgcgttcatg
     1501 caactaaaca gtgcctcaaa gcaccggcgt taaccttgtt aaatgatcaa cgtttacgcg
     1561 cttgttttgt ccctgccgag caattaatcc gaggtaacgc atcatgaaca cagagaaaaa
     1621 agtgatccta gaagtgaagg atttaagcgt tcatttcaac atcaaagata cgaaatcttt
     1681 attctttgct aaacaccaaa ctttaaaggc cgtcaatcat gtctcttttc agttatacga
     1741 aggcgaaacc ttaggtgtgg tcggtgaatc gggctgtgga aaatcaactc tcgcacgcgc
     1801 gattattggc ttagtggaaa gctctggcgg taatatttta tggctaggta aggagttaac
     1861 tcaacaaagt gcgaaagaat ggaaaacgat ccgtagcgat attcaaatga ttttccaaga
     1921 tcctttagcc tccctgaatc cacggatgac cattggcagt atcattgccg aaccattaaa
     1981 aatttaccaa ccccatttaa ccgcggaaga agtgaaagaa aaagtacgcg cgatgatgat
     2041 gaaagtgggc ttattaccta acttaatcaa tcgctatcca catgaatttt cgggtggaca
     2101 atgtcagcgt atcgggattg cgcgcgcctt gattgtggaa ccaaaactca ttatttgcga
     2161 tgagccagtg tccgccttag atgtttcaat ccaagcacaa gtagtgaatt tattgaaatc
     2221 tttacaacgc gaaatggggc tatcattgat tttcattgca cacgaccttg ctgtggtgaa
     2281 acacatttcc gatcgtgttt tggtcatgta tcttggtaac gcggtggaat taggtgacta
     2341 tgaacaagtc tatcacgata ctaagcatcc ttataccaaa gctttgatgt ctgcggtacc
     2401 gattcctgat ccgcaattag aacgcaataa acaaattcaa ttgcttgaag gcgatctccc
     2461 ttccccgatt aatccgccgt cgggctgtgt gttccgcacc cgttgcccac tggcagatca
     2521 acactgtatt gcagacaaac cgctcctcaa aggacgagat aatcacgttg ttgcttgttt
     2581 taaagccgag taatacacac aaccgcacaa aaaagtgcgg ttgttttttt gcgaatattc
     2641 acagtcagac tttcgtgata ctttcgctct gagataagga ttacgataca gagaagttat
     2701 tcaacaatca cacgtgcatt gatcggttgc actggacgat tattgtgtcc tttcatcaag
     2761 gtcgcaaacg cctcaacttg agattggcta atgttgtcat agtgttttaa cactaaccaa
     2821 tttacacctt cagaacaagg tggtgtagtt aatgacccgc tgaaacgata gtaatcgcgt
     2881 tttttcggaa ttaatgttgc tgggtcaaaa gcggtactaa gcgtatattt ctcccctgct
     2941 ttttgcggga agttttgcca aattggggct aaccattggt tttcacttga taatacatag
     3001 agcacgccaa gcacagcaag attcccttca ttgtctgcgt gtacaaaatg tgcttccatc
     3061 gggaaggatt tacctcaaat taagttttca ctcggcacat ggaaatggaa ttgtttaagg
     3121 gtaaaagtct ttccattaag ctgcatgcga ttacttgcct ctttgaaatc cacttgaata
     3181 gtatgcccat tattgacaat ctccacattg gaaggtcgat aatccagctt aatcggcgct
     3241 aacttaccat caatcgtatg cttgatatct attggtgttt ggtttttacc ttcgccacaa
     3301 atacggaatt tgggtgaaag ttcagcccaa tgttctggcg attcgtgtcc ggtgtatccc
     3361 caatgcgtgt gcgtttcttc tttcttaagg gggcttgtac acgctgtcaa tgctaatgca
     3421 cctaacgtaa gcacaaaaag gtttgatttt ttcatagcct agctcctgat taaaagagat
     3481 gaattgatga gacaacctaa actcaatgat gccttaatcc gagacgcttt catcttaggc
     3541 aaaacaacac tccatttcaa gtataacttg ctcaagaata tatcttagac cgaaatcaaa
     3601 tccattattt caaataaggt attaggcgag tatttctcta aataacgacg taaaaaatgt
     3661 tgtgtttctt ggctaatgac ttgatctgtt tccggataca agttgtatat cacagcatgc
     3721 acacttactc ttttggagtt gagtacttgt aaactcaata aggtatgatt aatactacct
     3781 agcttgccag aggtaactaa aatcacgggg tattgatgct gacaaagata atcaagggtg
     3841 gtttcttcct cattataagg tacacacaag ccgcccgccc cttccaataa tacataatca
     3901 tatttttcac acaaaagtgc ggtgcaggtt tcaatttttt tacgttcgat cgtgcgcggt
     3961 tctaatttcg cggctaaatg cggtgagcaa ggataatcaa acacgtaagg acaagtgata
     4021 ccttgcttat cttcttctgt aagctcaata ccttgtattt ttcggtgtat gagtaaatct
     4081 tcggcaattc ctcggcagcc tgtttggatc atcttttgcg tgatgacact tgcgccttgt
     4141 tgcatcagtt tcttggcgta ccaacctgtg gcgatggttt tgccaatatc ggtgtcgatg
     4201 ccagtgataa aaaaaacctt tgccatactt tttccttcta agattaagtt aaacgcgcca
     4261 gcacatacat cggcgtgtaa gtgagttcta ctttgtgttg tgcgttacgg taatgttgtt
     4321 gatattgttg gcaaaaggcg tgcaattgcc cttttgtcca cctttgctgc tgtgtggcag
     4381 taacgcctgt ttgtttcaaa tgttttaaca ccgctaaggg atcatcaaag tgcaacacaa
     4441 tctcctcgtg ttcgagggct aaaatctcaa attcaggcac tagccacttt tgccagtcct
     4501 caagacgtgg ataagttaac cccacaccgg tgaggtgttt gatctgtgtt aaattctcgg
     4561 gcgtgaaagt actgaataac aaaaatccgt gcttttttaa gcgcacttta caacgcgcaa
     4621 taaaacccgc tttatcttca aaccattgca ccacggaagc actggcaatc aaatcatact
     4681 gctgcggaaa gggaagttgt tccgcatcgc cacaatggaa gtgaaaaggt tggggcaaca
     4741 ctgattgcag atgctctcgc atatcgcaaa ggtcattcag ctcccagtgt tcaacctgca
     4801 actgtgacat taattgcttc gttaatccac cagtaccaca tccaatctct aatgcacgtt
     4861 ggaatgcttg tctgtcgata tacgacaata aactaagcaa acgttggtga atctgctgtt
     4921 gtgctctcgc ttgctgatca tagttcgata cagcggcaga aaaacgctgt ttaatgcgtc
     4981 ctttatcaat acgatccatg tgctacaacc aacaagtttc ccaatgggtg aagtgaggaa
     5041 acaacaaatg ctcccctgcg atttctgtta ttgcacaacg atcctgccaa taagcccgct
     5101 gattttgtgc agggaaaatt ttatcttttg agccgacaat cgccttgctc cacgagatta
     5161 aatctgtacg gtgatcttgt tgtaaaaagg tataaagtgc ggtcagttca gcatgaattt
     5221 cctgtaatgt gggacgcatg gaatagtgct gataatctct taacagcgtt tttgaaccac
     5281 aaatacgacg ttcaaattta gtccgattat cttcatttaa accagataac gtgccagcaa
     5341 aaatggcttc gggaatacca tattggtcat cacaaggtaa gcccgtgcca ttgatcgccg
     5401 tagcagatag cagtggataa ccttgcatca cacgctccgc tacccacacg cccattgacc
     5461 atgccaccac gcgaatatgt tgataagcgg aaaaatcaac atcgcaatgc agatctcgat
     5521 aatcatagca aaggcataaa tcataatttt ccggcagaat caggtgctcg accacgctcg
     5581 gcggtgtgcc ccaccctgca aaatagacga tcagattgtc gccttgctgt gaaatatgtt
     5641 gtgttttcat tcagcaagct cccgtaaaca agcaataaat tgttccactt ccgccacttg
     5701 catatctgca gtcagtgaaa aacgaatacg agaggttcct tttgggactg tcggcgggcg
     5761 aatcggtaag caataatagc cttgttgttg caaacgttgc gccgttctta ctgtcagttc
     5821 gttatcgccc aaaatataag gtacgataca actttcactt ggcatcggga cttgaaaaat
     5881 gtcaacaatc gcttggcgta aatgttgact aagctgagca agatgtgcgc gttcggcttg
     5941 caaatgtggt aattgttgaa agacaaaatg cgtccatgcc acattaaacg gcggaagtgc
     6001 cgtggaaaaa atcaatgggc gcattttatt aaccaaataa tctcggatca cttgatcgca
     6061 aatcacataa gcccccatag agcccaacgc cttgccaaac gtaccgacta aaatatcaat
     6121 ttggttgata cagccttgtt gttctgctaa ccctagcccc ttctccccca atacgcctat
     6181 cgcatgggct tcatctacat ataacatcac ttgcggatag cgctgtttta atgcgaccaa
     6241 ctgggtgagg tctgcacagt caccatccat actaaacacg gattccgtga ccacgataat
     6301 ccgctcaaaa gtgcgatcat ttttttgcaa aatttgttca aggtggacgt aatcatgatg
     6361 acgaaaacgc acaaactcac attgtgccaa gcgaatgcca tcaatcatac tggcatgtac
     6421 cagtttatcc gccacaatca gcgttttttt gtccgctaaa gcaggcaaaa tcccaatatt
     6481 agcatgataa ccgctattaa atagcaaggc agtttctcgt ccaaatgcct gcgccattaa
     6541 gctttctagc tcatcataaa tcggaaaaga gcctgttaat aaacgggagg aagaagaagt
     6601 caatgcaggg agttgatctt ggtattgcgt gaaaaatgcc tgacgcaagg cttcgttatt
     6661 ggctaaacct aaataatcat tggaggacat attcagcatc gtattgtcct cacgttgaat
     6721 aaaacgccct cggtgaatta gttggggcag ttggcgaaat tgattctgcg cctttaaatc
     6781 agctaactgc tgctgataat acctgatcat agagattcct tatgtgcata ttcttcttga
     6841 agggtgcgaa ttgtgccatg aattaattga gacaattgat ccgatgagat gatatacggt
     6901 ggcatcaaat acactaattt gccaaatgga cgcacccaaa cgccattttg cacaaagcgt
     6961 ggtggtaaac ttgccatatt gacaggcgcc ttcatttcaa tcaccccaat cgctccaagt
     7021 actctcattt cagcgacaca atctaaggct tccgcttggc gcaattcttg acgtaattgc
     7081 tgttcaatct gttgaatacg ttgttgccaa gggcttgcca ataacaattt gatactttct
     7141 gctgctaccg cacacgccag tggatttgcc ataaacgtcg gaccgtgcat aaaacatttt
     7201 gcctcgccag aacaaatcac gtctgctacc gcttggttgg taatggtagc ggatagagtt
     7261 aaataccccc ccgttaaagc tttaccgata cacatcacat ccggcacgac attggcatgt
     7321 tcacaagcaa ataatttgcc tgtacgccca aatcctgtgg caatttcatc aaaaatcagc
     7381 aaaatgccgt acggttcgca taaggctttt gcttgcacca aatactcagg ggaataaaaa
     7441 tacataccac ccgccccttg cactaccggt tctaaaatta aacccgcaat ctcatcacca
     7501 tgttgtgcca ataaatcctg taacggttgc atcgcttcgg gttgccaagc ttgcccaaat
     7561 tttaccgggg gttgcggtaa aaaatattgc acgggtagcc ctttggcaaa caagctgtgc
     7621 atgccggtca cgggatcaca cacggacatc gcatgccaag tatcaccatg atagcccgaa
     7681 cgaatcgtag caaatttata gcgctgtgtt tcgcctttag catgttgata ttgcactgcc
     7741 attttcatcg ccacttccac ggcgaccgaa ccactatcgg caaagaaaat tttatccaaa
     7801 ctttccggca agatgttgac cagttgctgc gccagttcta ccgctggttg gtgcgttaat
     7861 ccaccaaaca tcacatgact catttgactc agttgttgcg tggcagcgtg gtttaaacgg
     7921 gggtgattat agccatgaat cgctgaccac cacgatgaca tgccatcaat caactgctga
     7981 ccgtttttta actggatata cacaccttcg gcacgttcta ctgcaaataa gggaaaatcc
     8041 gcatttaacg ccgcataagg gtgccaaata tgttgtcgat cacattctaa aagttgctgc
     8101 tcattcatcg tttcacctcg gttgtgaatc gccctttcaa ctggcgtaaa ttcacgcaaa
     8161 tacttacata aacggaataa cttgttataa tcgccaagca tttggctt
//
LOCUS       DANLUA010000111         8194 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLUA010000111 DANLUA010000000
VERSION     DANLUA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(345..2015)
                     /gene="glnS"
                     /locus_tag="PXB44_001788"
     CDS             complement(345..2015)
                     /gene="glnS"
                     /locus_tag="PXB44_001788"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL5282244.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2176..2619)
                     /locus_tag="PXB44_001789"
     CDS             complement(2176..2619)
                     /locus_tag="PXB44_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5282245.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2670..3245)
                     /locus_tag="PXB44_001790"
     CDS             complement(2670..3245)
                     /locus_tag="PXB44_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL5282246.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3352..4242
                     /locus_tag="PXB44_001791"
     CDS             3352..4242
                     /locus_tag="PXB44_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5282247.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4309..4875)
                     /gene="efp"
                     /locus_tag="PXB44_001792"
     CDS             complement(4309..4875)
                     /gene="efp"
                     /locus_tag="PXB44_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL5282248.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            5042..5611
                     /locus_tag="PXB44_001793"
     CDS             5042..5611
                     /locus_tag="PXB44_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282249.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            5665..6657
                     /gene="epmB"
                     /locus_tag="PXB44_001794"
     CDS             5665..6657
                     /gene="epmB"
                     /locus_tag="PXB44_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL5282250.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(6757..8160)
                     /gene="asnS"
                     /locus_tag="PXB44_001795"
     CDS             complement(6757..8160)
                     /gene="asnS"
                     /locus_tag="PXB44_001795"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL5282251.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
BASE COUNT         2339 a         1770 c         1706 g         2379 t
ORIGIN      
        1 atcataaaag agcatcactt ttggtttcca aagattatag aaagctcaaa taatacacta
       61 cctcataatt taaaatgaaa cgtttcaatt ggaaaaagtg gattttgtga ctgagatcac
      121 aaatttaaac attggtctgt atttttgaga gattttgttt gaaactgccg gcaaattcgc
      181 ttaaccttaa ggtaacaaga ataagtgttg tacctagcaa cctaacacat aaatttgctt
      241 tccgagtggc cccaagaatt ggggcttttt ttatgcttga tgagtcgttt tgagagtaaa
      301 aaaataccag catatttgct ggtatttgaa tgaagagagt tagattagaa cgctacgctt
      361 tcttttaagc tgacagttaa gttaaataca aggttgtctg ccgaaccatc tttgctgtct
      421 aagcagtagt aaccttcacg ctcgaattgg tagtcttgtt ccggtttggc gtgagctaaa
      481 cttgactcaa caaaaccatg cttaaccact aatgattcag gatttagcac cgcattgatg
      541 tcttcttccg cccccgggtt tggtactgta aataggcggt cgtaaatgcg gaactctgcc
      601 ggtttgttat cttcagcaga tacccaatga atcacgcctt ttactttgcg accatcagcc
      661 ggatttttgc ccaatgtatc cgggtcgtat gtgcaaaaaa tagttgaaat tcgaccgctt
      721 gcatcttttt ccacacgctc agctttaatc acataggcat tgcgtaaacg aacttctttg
      781 cctaagacta aacgtttata ttgcttattg gcttcttcac ggaagtcggc ttcatcaata
      841 taaatctcac gggtgaacgg taaatcacgc tcgccaagct ctggacgatt tgggtgatta
      901 ggggctttta atatttcctt ttcaccgaag ttttcaatca ccactcgaac cgggttaatc
      961 accgccatcg cgcgtggtgc gttttcattt aagtcttcac gaatgcacgc ttcaagggca
     1021 gagtattcca ccacgttatc ttgcttggtt accccaatac ggcgacaaaa ttcacgtaaa
     1081 gacgctgggg tataaccacg gcggcgtaag ccggaaatgg ttggcatacg agggtcattc
     1141 caaccatcaa caatgccgtc tgtgactaat tttaataatt tacgcttaga ggttaaagta
     1201 ccttctaaat ttaaacgtga aaattcgtat tggtgcggta acggtctttc aatcgaaata
     1261 ttgtccagca cccaatcata taagcggcgg ttatcttgga actctagcgt acatagcgag
     1321 tgagtgattc gctcaatcgc atcggaaatg cagtgggtaa aatcgtacat cgggtagatg
     1381 caccatttat caccggtttg atgatggtga gcaaatttca cacggtaaag caccggatct
     1441 cgcatcacaa taaacgggga cgccatatca atttttgctc tcaagcaggc tttcccttcc
     1501 gcaaactctc cgtttttcat tttttcgaat aacgctaagt tttcttccac gctacgatca
     1561 cggtaagggc tgtttttgcc cggctcggtt aaagtaccac ggtattcacg catttcatca
     1621 ggcgataatt catcaacata ggctaatcct ttgttgatta actcaatcgc atagccataa
     1681 agctggtcaa aatagtctga ggcataacga ggctcaccct cccatttaaa acctagccac
     1741 tccacatctt gcttaattga atctacatat tccacgtctt ctttaaccgg gttggtatca
     1801 tcaaaacgca agttacataa accgttataa tcttgagcga taccaaaatt taaacagata
     1861 gatttagcgt gtccgatatg caaataaccg tttggttcag gtggaaaacg agtgtaaaca
     1921 ttgttgtgct ttccgcttgc taaatcttca tcaataatat gggtaataaa attggctcga
     1981 gtgccagttt ctgccgttaa aatttcttcg ctcatagttc tctctaaatg tatcaataaa
     2041 aataacggat tattctacac ttaattgaaa cagacttaaa gactaaagca aaattcctcg
     2101 gtttcctcca aagaattttg ctgttttagt aagatgcaat ggaattgcaa atttttagct
     2161 aaatttaacc gcttgttatt gcctgatttc acggctttcg ctgatcataa aattcgcaat
     2221 ccgtttggca atccgccatt taccctcttg tttaacatag gtatcggcat aacgcacgct
     2281 gtgatcgtgc aagatctctt tgccctcttt ggtttctacc agtttcaccg cacaatagtt
     2341 gatcgctgtg gcgttatttt catcaataaa aggcacggta tgctgaccgt ttaaatgata
     2401 aacgctgtga aacggggtta aaaaggtggt aaacacctgt tcgatttctg ccacgccgtg
     2461 catttcaaac accaattttc cgccgatgta ggtgtttacg acggcatctt gcgtgaaaag
     2521 cggcatctgc tcggcaacct ttttttcatc ggcaaggtta gagaatgtat ctactaaatc
     2581 ttttagtgct aaacgatctt ggatttgttg taagttcatt gtgttttcct tctgattttt
     2641 gcaaattttc tttggttttt caccgcttgt taggctgcaa taccaacact ttcacggatt
     2701 tcgctgtttt cacgcaaaat gcgagccaca aaatcggcaa tggatttgcg ggaaacgctg
     2761 tgtcctttga acggttcgcc tttgtgagtc agctgatatt gcacttccga accgttggta
     2821 aaccagcccg ggcggacaac ggtataatcc aactcgctgg cttcaatcac ggctgccgat
     2881 tggcggtaag gctctaaaat tgccccgtga tcttcgccgg tttcgccata aatccccatt
     2941 gagctaatcc aaaccaagcg ttttacctga gcttcattca ttgcgttcac cacatttccc
     3001 gccatcgctt ttaagtcgcc ggcaagtcca gcgtaaacgg catcaacgcc ttgcattgct
     3061 tgggttaatg cgtggaaatc caacacatcg ccttgcacaa ttttgacgcg ttcagtttca
     3121 aatttcgcta ctgattgcgg acgacgggca aaaagggtta attgcacatt attgtctttt
     3181 agcagttcag gaatcacgac attcgctagg ctaccgcttg ctcctaaaat taagatctgt
     3241 ttcattgctg ttctccattg aatgaagtca gtatagcggt gtagattgca tttgattagt
     3301 agggtaaaat cgcttgaagt aatgagaaaa attcattaat aaggtggtta aatgaaagaa
     3361 aacctcaacg atttaagagc ttttttgttg gtagcacaaa cgagcagttt taccaaagca
     3421 gcagcacaga tgggcgtgtc gcagtcggcg ttgtcgcaca gcattcgggg gattgaagaa
     3481 cggttgcaaa tcaagctgtt tcatcgcacc actcgtagca tttcaaccac cgaggcaggg
     3541 gagaggctgt atcaacggct gttaccgctg tttgatgaaa tcgaccaaga agtaaatggg
     3601 ctgagtgagt ttcgcaatgc gttaaaaggc gggctgagaa ttaacggtaa tgaacacgtt
     3661 ttccgtgatg tgctggcaga taaattcgct gagtttgtca aacgttaccc tgaagtagca
     3721 ttggagctag tggcggaaga tcgctttgta gatattgtcg ccgagcgttt tgatgccggt
     3781 attcggcttg gggcagatgt ggcgaaagat atgattgcac tacgcatttc gcccgatgtg
     3841 aaaatgaccg cagctgccag ccccaactat ttggcacagc acggcacgcc gaaaacgccc
     3901 tttgatttaa ccgaacatca ttgtctcaaa catcgtcttg cgacattggg cggcattctg
     3961 gcgtgggaat ttcgggatcc catcaccaag aaaatcgtta aatttcaacc gcagggcaat
     4021 cttactgcca ataacggctt cttgctgcaa cactatgcca aacaaggttt aggcattctt
     4081 tggagtccgc aagacagcat tcaaaccgag ctggataacg gcagcctcgt gccgatttta
     4141 accgactggg caatgcagta tgagggctat catctctatt accccaaccg tcgccaaaat
     4201 tcgcctttgc tgaaagcgtt aattgatgtg ctgagaggat aggtaaaaat gagcggtcgt
     4261 ttttagcaaa aaattgacca aaaaacgacc acttgtagag aagctaaatt atttcacacg
     4321 agaaacatat tcgcctgaac gggtatccac acggataact tcaccgattt gaacgaataa
     4381 aggcacgttt accacagcac cggtgcttaa tgttgccggt ttaccgcctg tacctgcagt
     4441 atcgcctttt aagcccgggt cggtttcgac gatttctaac tcaacgaagt ttggtggtgt
     4501 gactgcaatt gctgaaccgt tccataaagt cacgatacat tctgcattat caactaacca
     4561 ttttacgttg tcgcctaacg ctttttcatc taccgagatt tgttcaaagg tttcagggtg
     4621 cataaagtac cagaaatcac catcgctgta agagtagttg tagttagaat ctactacatc
     4681 tgctgcttca accgaagtgc ctgatttgaa gttgatctct aatactttgc cagagattaa
     4741 tttacggatt ttagtacggg taaatgcttg acctttaccc ggttttacga attcgttttc
     4801 aacaattaca caaggttcgc catcttgaat aaattttaaa ccttttttaa agtcgttagt
     4861 gctgtagcta gccattattt cctcaattat ttaaaaaata acaataaatt aacaaatttt
     4921 gaacccaacc ctattgggtt tgtcaaaaag agccatataa taacctaaat tcacaaaatt
     4981 agcgaaagat ctcttgcgat ttttgctata atttttcaaa ctttattgat aaggaatgcg
     5041 aatgaaaaaa ctgattactc tttctgcgat tttagccgtt tctgttgcaa ccgtggcaga
     5101 ggcaaaacgt ggcggtggga gcttccgttc agttagcccg tcaaaagcgg tagcaactaa
     5161 gccggtagct tctcaaaaat caaccacaaa tcagcaaacg caacaaaaag atgctacttt
     5221 tgccaatacg ccaaacagtg caactgccca gccacaagtg cgtcaaggca gcacgatggc
     5281 aaatgtagcg atgggggcag cagccggtta tattttaggt gatatgatgt catcaaatgc
     5341 acatgcaaat cagcctagcc agcctgcgga tgcaaatgca caagcggtca attctgcaca
     5401 aaatgttgca aatggagcag ttcctgctat tgcagaattt aagagtattg gcggtcaaat
     5461 cgatccgttt ttagtagaaa aaaccgatgg ttatcgccgt tattgtattt caggcgtgca
     5521 atacctagtc gctgcacaag gtgtacaaac ctcaccaact gtaatggtta atccaaatgg
     5581 cacgccatta caatgtaact taattccata attattttta tttgcgggca ggcaaaaacc
     5641 tgccccttgt atctacaata ttcaatgcaa ccaattcaaa tcaaaccgct ttggctaacc
     5701 gagctggctc aagcctttaa taaccctatc gacttattac aatttcttga actcaatcca
     5761 aatgattttg agggcgatat agctgcccgt aaattatttg ctcttcgagt gccaagaatg
     5821 ttcgccgaaa aaatggaaaa gggtaaccca aacgatccgc tttttttgca agcaatgtct
     5881 ttacaggcgg aatttattga ggcagaaggg tttgtggtag atccattgga agagcaacaa
     5941 agcccagcac cgaatatttt acataaatac cataaccgtt tgttatttat gctaaaaaac
     6001 agttgtgcga ttaactgccg ttactgtttt cgccgtcatt tcccttatga ggaagtgaaa
     6061 agcggtaaag ctgtgtggca acagggacta acttatattg ctgagcatcc cgagctggag
     6121 gaagtgattc tgtccggtgg tgatccgctg atggcaaaag atcaggactt agattggata
     6181 ttaacgcaat tagagcagat ttcgcatatc aaaaccctgc gtattcattc acgcttgccg
     6241 gtcgttattc ccaatcgaat tacgacagaa ttatgtgaac ggttatcaaa atctcgcttg
     6301 aatgtcgtgt tggttaccca tattaatcac gccaatgaaa ttgatgctgt ttttgcaaac
     6361 aaaatggctc aactgaaaaa agccggcgtt gtattgctga atcaatcggt gatgttaaaa
     6421 ggtgtaaacg ataatgctca aaccttaaaa aggttaagtg ataaactttt tgaatatggg
     6481 attttgcctt actatttgca tttatttgat aaagtggcag gggcgagcca tttctatatt
     6541 gaagatgaac aggctggtga gatttaccga gaattacagc gtatcacttc aggctatctt
     6601 gtgccaaagc tggcaagaga aatagcgaaa gagccaaata aaattttgaa aggttagctt
     6661 aaacagaaat caagaaacaa aaggcgaaaa tggtaaaatt ttcgcctttt ttaaccgctt
     6721 gttgcaagcg gttaaattct cttacttttt tgcaaattag aagttcgcat tgcgtggagc
     6781 acgtgggaat gggattacat cacgaatgtt ttgtaacccg gttacataca cgattaaacg
     6841 ctcgaaacct aaaccgaaac ctgagtgtgg cactgtaccg tatttacgca aatcacgata
     6901 ccaccagtaa tcttcagggt ttaagcccat ttcattcata cgctgatcta acacttctaa
     6961 acgttcttca cgttgagaac caccgataat ttcaccgatt cccggagcta aaacgtccat
     7021 tgcggcaacc gttttgccgt catcatttaa acgcatatag aaggctttaa tgtcttttgg
     7081 atagtttttc acgaccaccg gcgatttaaa gtgttcttcg gctaagaaac gctcgtgttc
     7141 agaggaaaga tcgattcccc aagacaccgg gaattcaaat tctttgccgg atttcaataa
     7201 gatctcaatc gcatcggtgt aatctacttg agcaaatggc gaattgataa agttttctaa
     7261 acgactaatc gcttctttat caacgtgttt aacgaaaaat tccatatcat ctttacgttc
     7321 tgcaagcacc gctttaaaca catatttcag catatcttcg gctaattttg cattgtcgtt
     7381 taagtcggca aaggcaactt ccggctccat catccagaat tctgcaagat ggcgagttgt
     7441 gtttgagttt tctgcacgga atgtcggacc gaaagtataa actttactta aggcacacgc
     7501 ataggtttcc gcatttaact ggccggatac ggttaagaac gattctttac cgaagaaatc
     7561 ttggttaaaa tccactttgc cgttttcgcc acgaggtaga ttttctaaat ccagcgtaga
     7621 aacacggaac atttcacctg caccttcagt atctgaggcg gtaattacag gtgttgctac
     7681 ccaatagaaa ccttgctcgt ggaagaaacg gtggatagct tgagctaaac agtgacgaac
     7741 acgagcaact gcaccgatta agttcgtacg agggcgtaag tgagcgactt cacgcagata
     7801 ttcgatagag tggcgtttcg ctgccattgg gtaggtatca ggatcttcca cccaaccaag
     7861 cacttccact ttacttgctt gtaattcaac ggcttgacct tcagcagggg attccacaac
     7921 tttaccggtc accaccacag agcaacctgc ggttaaatgt aatacttcgg cattataatt
     7981 tggtagatcg ttattaataa tcgcttgaat tgggtcaaag caagaaccat cgtaaacgct
     8041 caagaaagat aaaccggctt ttgaatcacg gcgggtacgc acccaaccac gcactgtcgc
     8101 ttcttcacct accgctatct tgccggatag gatttccgaa actgttggaa atttagacat
     8161 tgaatacctc aaattctttg ttgtgagttt taat
//
LOCUS       DANLUA010000112         8176 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLUA010000112 DANLUA010000000
VERSION     DANLUA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            46..819
                     /locus_tag="PXB44_001796"
     CDS             46..819
                     /locus_tag="PXB44_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5282252.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            902..1618
                     /locus_tag="PXB44_001797"
     CDS             902..1618
                     /locus_tag="PXB44_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL5282253.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            1628..2380
                     /locus_tag="PXB44_001798"
     CDS             1628..2380
                     /locus_tag="PXB44_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL5282254.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(2596..3129)
                     /locus_tag="PXB44_001799"
     CDS             complement(2596..3129)
                     /locus_tag="PXB44_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL5282255.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(3235..3576)
                     /locus_tag="PXB44_001800"
     CDS             complement(3235..3576)
                     /locus_tag="PXB44_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL5282256.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(3600..4349)
                     /gene="trmB"
                     /locus_tag="PXB44_001801"
     CDS             complement(3600..4349)
                     /gene="trmB"
                     /locus_tag="PXB44_001801"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL5282257.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(4359..5309)
                     /locus_tag="PXB44_001802"
     CDS             complement(4359..5309)
                     /locus_tag="PXB44_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL5282258.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(5455..6474)
                     /gene="hemB"
                     /locus_tag="PXB44_001803"
     CDS             complement(5455..6474)
                     /gene="hemB"
                     /locus_tag="PXB44_001803"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL5282259.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(6555..7310)
                     /gene="tatC"
                     /locus_tag="PXB44_001804"
     CDS             complement(6555..7310)
                     /gene="tatC"
                     /locus_tag="PXB44_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL5282260.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(7297..7941)
                     /gene="tatB"
                     /locus_tag="PXB44_001805"
     CDS             complement(7297..7941)
                     /gene="tatB"
                     /locus_tag="PXB44_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL5282261.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(7945..8166)
                     /gene="tatA"
                     /locus_tag="PXB44_001806"
     CDS             complement(7945..8166)
                     /gene="tatA"
                     /locus_tag="PXB44_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL5282262.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
BASE COUNT         2316 a         1857 c         1636 g         2367 t
ORIGIN      
        1 ttaaatttgc aaaaaattta ttttaccaat taaaaggaaa tgattatgtt gaaaaaatta
       61 tcaattgctg cattagctct taccctttct gtttctgcat ttgctaaaga gagcctactt
      121 gagcgtataa acaacaaagg gactattact gtgggtacgg aaggaactta tgccccttac
      181 acctatcacg atgcgagcgg taaattaacc ggttacgatg tagaagtaac ccgtgcggtg
      241 gctgaaaaat tagacgtaaa agtggaattt aaagagacac aatgggacgc tatgttagcc
      301 ggtttaaaag gtgagcgttt tgacttagtg gcaaaccaag tggctttaac cagcccggaa
      361 cgtcgagcga catttgataa atccgaatct tacagctggg acggtgccgc cttattagtg
      421 cgtgaagatg aaactaaaat caaaacccct gctgatgtaa aaggcgtgaa aactgcccaa
      481 accttgagct caaactatgg cgagattgcc cgtgaaaacg gtgcagacat cgtgccaatt
      541 gacggtatgg ctcaaggctt actgttaatt caacaaaaac gcgccgatgc aactttcaat
      601 agccacttat cgctattaga ttacttgaaa aagaacccga attccggctt aaaaattgtg
      661 tgggaaaaag aagaaaaagt gggtgcaggc ttaattgcga ataaaggcaa tgacgaagca
      721 ttagcgaaaa tcagcaaagc aatcgtagaa ttacgtgaag acggcacgtt aaaaaaattg
      781 ggcgagcagt tcttcggtaa agatattagt gtacaataat ccatattgga ttaggcttgg
      841 ggcgaattcg ccccttttat ttttaaatct ccctcaaaac gggagatttt aggtttttca
      901 gatgttaaac gatctacttc tctcaatccc ctttatgacc caagctcggg tcgatttagt
      961 gctaagcggt ttttggccga tggtacaagc ggcattttta gtctctattc cgctggcaat
     1021 cgcctcattt attatcggta tgttaattgc cgttgcagtt gcattagtgc gaattagccc
     1081 tgcaacaggc ttttttcacc gcttgctact gtggctggta aaaggttata tctcgattat
     1141 tcgcggcacg ccgatgttgg ttcagatctg tattgtgttt tatggtttgc ctgcaatcgg
     1201 catttttatc gatccgattc cggcggcaat tatcgggttt tcactcaata tcggggctta
     1261 tggctcggaa acgatccgtg ctgcgatctc ctccgtgcca aaaggacaat gggaagccgg
     1321 ttatactatc ggtatgactt atatgcaaac ttttcgccgt atcattgccc cgcaagcgtt
     1381 tcgagttgct gttccgccac ttagcaacac ctttatcggt ctgtttaaag atacctctct
     1441 tgcctccgtt gttaccgtaa ccgaaatgtt ccgggttgct caacaggtag caaatatgtc
     1501 ttatgacttc ctacctgttt atattgaagc cggtttgatt tattggtgct tctgctgggt
     1561 gctattttta attcaggcga gattggaagt ccgctttaac cgatttgtgg cgaaataagg
     1621 aggaattgtg cttaaaattc gcaatattca taaaaccttt ggcaacaacg ctattctacg
     1681 cggcattgat ttagatattc aaaaagggca agtggtagta attttaggcc cttccggttc
     1741 aggcaaaacc acctttcttc gttgcttaaa tgcactggaa atgcccgagc aaggcacatt
     1801 ggaatttagt ggtaatgctc cgctgaaaat tgattttgcc gtaaaacaga gcaaaaaaga
     1861 gattttagcg ctacgccgca aatccggtat ggtgttccaa aactacaacc tgttcccgca
     1921 taaaaccgca ttggaaaatg tgatggaagg accggtattt gtgcaacaac aagcggtcga
     1981 aattgctcaa aaaaatgcaa aagccctgtt agaaaaagtc ggactggcgg ataaagcaga
     2041 actctacccg tttcaactgt caggcgggca acagcaacgg gttggcattg cacgagcatt
     2101 agcaattcag ccggagctga tgttatttga tgaaccaact tcggcacttg atcctgagtt
     2161 agtgcaagac gtgctaaata cgatgaaaga actggcaaat gaaggctgga caatggtcgt
     2221 ggttactcac gaaatcaaat ttgcccttga tgttgccgat gtagtggtgg taatggacgg
     2281 cggtgaaatc gttgaacaag gctcgccaca gcaactgttt aaccacccac aacacgaacg
     2341 aactaaacgt tttttaaatc aaatcagagc ggataaataa gaaacgatag cgctagcgtc
     2401 cacgctagtg ctatctaata aatggtacaa gcggttaatt atgtataaat tgttatactg
     2461 ttttggcacg agcgtggacg ctcgcgccat cgtacagtaa aatcagctaa caaggttccc
     2521 cataaaggat aaataagaca aggctcctta atcttttaga ttaaggagct tatttttaga
     2581 ttttagaaat ccgatttaag ccgcagccca tagtccacca aaaatttggt agaacaagct
     2641 atttagtagc aataagccga aacctagcac cattaccgag aaatctaaca ttcccgttct
     2701 tggcaggagc ttgcggataa aacctaacac cggttcggta atttgtgcta ccgtgtagtc
     2761 taatgggtgg ttgccttgag tgacccagct cattaatgca cgaatcaagg tggtgaaaaa
     2821 tagaatttgt ccgaaggttt tgaccacact taataagccg attaatactg ctctgggtaa
     2881 atctacgcca aacagaataa actgtaacgt taccaaagcg gcggcaatta atagtgccgg
     2941 aaagcaaata tttttaacgg ttggaatcag cttgccaacc ggtgaaacca tcggttcggt
     3001 aattttaagt aaggtttgtg aaatcggtaa acgaggatca acccgacaaa attgcagcca
     3061 agttcttagc accagcacaa aacttaaaaa gccaacaata agcgaaataa caggagctaa
     3121 aaattccatc tctttttctc tttctataca aaacaagcgg tcgtttttta gaaaaaattt
     3181 gcaaattttc agtaaaatct gaccgcttgt atcttattaa attaactagg caaattaagc
     3241 ttgtgctggg tagtcccacc aaatatcaaa taattcgctc acttgaacct cttttaagcc
     3301 gttagtttct aaccaagttt tcactaattg gcggtgagat tcatcacatt tgcctaattt
     3361 ttctaagcag actaaacctt cccagtttaa gtagccgttc gcttcaaggg ctaaaccgtt
     3421 tggtcgaatc acttcatcga taaaacgatc tacggtttca tcaatttggt caatgcctgt
     3481 gccttcagca aaggtgaatt taattaagaa acctaattct tggtattcac ctaagtgcat
     3541 ttttttacgt tggcgtgcgt tacgttgaat agccattttt tctctcctct atgaataaat
     3601 catttctttg taaaatataa atcccacacc ccgtgtccta aacgatgccc tcgctcttca
     3661 aatttggtta gcgggcggaa attcggacgt ggaataaaat cattagttgc tgatgtattt
     3721 tgcaactcgc tctcaaattg gcgtaatact tcaagcatat gttctgcgta attttcccag
     3781 tcggtggcaa aatgaataaa gccgttaggg cttaattttc tgatcacacg gctgataaat
     3841 tcaggctgca caatacggcg tttgtggtgt tttgcttttt gccaagggtc tgggaagtaa
     3901 agctgtaagc cacctaatgt accgtcagca atgctgtctc gcaaaatttc ggtggcatcg
     3961 tggcaaatta ctcttaaatt cttcacgcct ttttctaacg cataagcgat acaagccccc
     4021 accccgggcg tgtgcacctc aatgccgata tagttgcgat tcgggttttg ctctgccatt
     4081 tccaccagcg attttcccat tccgaagccg atttctaaca ctaccgggtt gttattgccg
     4141 aaaatctgct caaaattaaa gggcattggc tggtaatcta agcctaaatt tgcccagttg
     4201 ttgttcatca tatcacgttg ataatcgctc aaacgaccgg tacgcaatac aaaactccgc
     4261 acttttcgta aataacgccc atcttcggta aactcggctt gctcaacggt tttacgtttt
     4321 ttatctgcaa aagtctgttt ttctgacatt ttcttgcctt aaaacttcac taataccgaa
     4381 ccccaagtcc agccaccgcc gaatgcttca aggagtagta attgcccacg ttgaattcgc
     4441 ccgtcacgca ccgcttcatc taatgccacc ggcactgttg ctccactggt attggcatat
     4501 ttatcaaggg taaccaccac ttgatccatt tccatctcta attttttcgc cgttgcggtg
     4561 ataatgcgta aattcgcttg gtgcggaatc agccaatcta aatcggattt ttgtagatta
     4621 ttcgcttcta aggtttcttc taccacgctt gaaagttggt ttactgcaat tttaaaggtg
     4681 gcattgccct gcatttcaat aaagccggaa cgctcttcgc cacgcttttg gcttcttaag
     4741 gttaagttat tttctaaatc cggtgaggcg tgcaagtggg ttgaaataat acccagctct
     4801 tcactcgcct ctaaaatcac cgccccagct ccgtcaccaa ataatactac tgtgctgcgg
     4861 tcggtttcgt ctaacattct ggaattgatg tctgaaccaa taaccaatgc ctttttcact
     4921 tgcccgttac gcacgaattg atcggcaaca gttaaagcat acacaaatcc tgtacaagcc
     4981 gctgccacat caaaagcaat cgcgtcttca atctgaagca agccttgaat ttggcaagct
     5041 gcacttgggt aagcgtggga gttggtagtt gttcccacca caattaaatc aatgtcattt
     5101 gcctcaattc ctgccatttc cagtgcttgt tgagcggctt ttgcccccat tgttgccact
     5161 gtttcgtcag gctcggcaat acggcgttca cgaatgccgg aacgggttac gatccattca
     5221 tcagaggtat cgaccatttt ttctaaatca gcattggtac gcacctttgt cggtaaatag
     5281 cttcccgttg ctaaaattct actattcata tttttattat tctcaatcaa ttaaatgata
     5341 atccgttatt atagcttgaa aaccaacaaa tatataggct ttacaagcgg tcatttttag
     5401 caaaaaagtt gcattcacga acgattttgc actagaacca ccgcttgcta gtatttattt
     5461 cgcttgagcc tcttcgtata gccacttcgc tactcgcttg gcgaaataag ttagaatacc
     5521 gtctgctcct gcccgtttaa agcagagtaa tccctccata atgcactcac gctctttcag
     5581 ccagccgttt tggattgccg ccatatgcat cgcgtattcg cccgaaactt ggtaggcgaa
     5641 ggttggcacg ccaaaattct ctttcacacg ccacaccata tccaaataag gcatacccgg
     5701 tttcaccatc accatatctg ccccttcttg aatatccatc gccacttcgt gcaagccttc
     5761 gttgccgtta gccggatcga cttggtaagt gtatttatta ccgcctttta agttgccggc
     5821 agaacctact gcatcacgga acggtccgta ataattagac gcatatttag ccgaatacgc
     5881 cataatttgc gtattgataa aaccgttttg ctcaagggct tcacgaattt tgccgattcg
     5941 accgtccatc atatcgctcg gggcaacaat atccgccccc gcttctgcgt gcgataacgc
     6001 ctgttttacc aatacttcag tagtcacatc attcagtaca tagcctgttt catcaataat
     6061 gccgtcttgt ccgtgcgtgg taaacgggtc taacgccaca tcagtaatca cgcctaattc
     6121 cggaaattga gcttttaaag ctcgcacggc acgctgtgcc aacccttgcg ggttataggc
     6181 ctcttctgcc atcagtgatt ttttcacatc acccaccaca gggaaaagag ccaccgcagg
     6241 cacgccatat ttaaccagtt ctgcggcttc aactaaaagc tggtcgatag ttaaacgctc
     6301 tacacccggc atagaaggca cttgtactcg ctgattttcc ccttcgatga caaaaacagg
     6361 gtaaatcaaa tcattaacgg taagttgatt ttctgccact aagcggcggc tgaaatcgtg
     6421 cttgcgtaaa cgacgcatac ggcgtaaagg atattgggat tgaattactt gcatttttaa
     6481 tttcctaatt cttaaaatac ctattcttga aataagaaag gcatagcaaa caggtgctat
     6541 gccttttcaa aatattactg tgcaacctgc tctgatggtt catcatcatc tttggggcga
     6601 taaaaccgag caaatagcac accaacttca aacaataaac acattggaat tgccaataat
     6661 gtttgcgaga aaatatctgg tggggtgagc aacataccaa ttacaaatgc tgccacaata
     6721 atataagggc gttttgcctt taaatcttcg ggtgtggtta cgcctgccca acataacaag
     6781 ataatcgcaa ccggcacttc aaagcagatc ccgaatgcca taaaaatggt taaaacgaaa
     6841 tcaagatagc tactaatatc cgttgccatt gttacccctt ccggagcggt agaggttaaa
     6901 aagccaaata caagcggaaa tacaacataa taggcaaaag cgacaccaca ataaaacagc
     6961 aatgtgctag ataccaataa tgggtaaacc aaacgctttt cctgcttgta taaagcaggg
     7021 gcgacaaacg cccaaatttg gtacaaaata aaaggaactg ataaaaacac cgaaaccaca
     7081 atggttaatt taattggggt aaaaaacgga gtggctacat tggttgcaat cattgttgca
     7141 ccgtctggta aattgtccgt taaaggtgtt gccaataagg tgtaaatatc attggcccag
     7201 taaaccaatc cgcaaaacac caagagcaca cagataaaac tacgcagtaa tcggtttctc
     7261 agctcaatta aatgtgtaat cagaggttga gactcatcaa cttgcgacat tatttgcctc
     7321 cgatttctgc gctgattctg atgattgagg cgtagctaaa tcatcatctg gcggatagta
     7381 agattccaac gttaaaagcg attcgtccat ttctgcttgt tcagccagct catcagccga
     7441 aagtggcttt tctgtccgcg taacattaag ttgctcagat aaactatcca cttcaagcgg
     7501 tttattttgc gcattttttt gcaattcttg cgcctctaac acctggcttt cttgctcgat
     7561 tttttgctga atttctgcga cttgctcatc ggttaattta ggaattgtgc tgctttccgc
     7621 agccgttttt tccaactcat tttttgcaga attaagcgaa tcttgcaact gggtggctga
     7681 cattttcaga tcttccaccg ttttatttaa ttctggcgaa agggagctta aattcaactc
     7741 ttctgccttt ttaatgctct cttttaattc ttgcagtttc agctcttgag ccagttcatt
     7801 ttgtacgttt gcggctaaac ctcgaattgt actcacccag cccattacgg tacgaatcgc
     7861 aatcggcata cgtttcgggc ctagcaccac caagcccaca atcataatca gtactaattc
     7921 agaaaaacct atatcaaaca cggattaagc ctgctctttc tcttttgctt tctgctctgc
     7981 tgaaattgca tcttttgctt ccactttttc aaattcagca tcttttttct catctgccat
     8041 tgcttttttg aagcctttta cagattcgcc caaatccgaa cctaaagtac gtaatttctt
     8101 cgtaccgaaa agtaatacga tgattgcaac cacaatcaga agctgccaaa tactaatacc
     8161 gcccataaaa actcct
//
LOCUS       DANLUA010000113         7919 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLUA010000113 DANLUA010000000
VERSION     DANLUA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            46..3117
                     /gene="fdnG"
                     /locus_tag="PXB44_001807"
     CDS             46..3117
                     /gene="fdnG"
                     /locus_tag="PXB44_001807"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:631..633,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL5282263.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3120..4034
                     /gene="fdxH"
                     /locus_tag="PXB44_001808"
     CDS             3120..4034
                     /gene="fdxH"
                     /locus_tag="PXB44_001808"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL5282264.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            4027..4704
                     /locus_tag="PXB44_001809"
     CDS             4027..4704
                     /locus_tag="PXB44_001809"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL5282265.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4760..5677
                     /gene="fdhE"
                     /locus_tag="PXB44_001810"
     CDS             4760..5677
                     /gene="fdhE"
                     /locus_tag="PXB44_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL5282266.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(5747..6133)
                     /locus_tag="PXB44_001811"
     CDS             complement(5747..6133)
                     /locus_tag="PXB44_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282267.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFMSDREIIANRCEVKKIAD"
     gene            complement(6144..7514)
                     /gene="gorA"
                     /locus_tag="PXB44_001812"
     CDS             complement(6144..7514)
                     /gene="gorA"
                     /locus_tag="PXB44_001812"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL5282268.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            complement(7591..>7919)
                     /locus_tag="PXB44_001813"
     CDS             complement(7591..>7919)
                     /locus_tag="PXB44_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL5282269.1"
                     /translation="LIDVVMIANLLVMVIVGGYETFVSRLKVDDHPDQPEWLDHVNAT
                     ILKVKLSMAIISISSIHLLQTFINASNLDEKQMMWQVVIHLAFIVSALAMAYIDKMLN
                     SGHMKH"
BASE COUNT         2382 a         1734 c         1651 g         2152 t
ORIGIN      
        1 atcaaatttt gttttatttc tcacattatg tgatttggag tgattatgca gatctcaaga
       61 cgtaagttct tcaaggtctg tgcaggtggt atggcgggaa cttctgctgc aatgttgggt
      121 tttgctccag caacagcatt agctgcaccg cgtgagtata agctcttaag agcaaaagaa
      181 actcgacaaa cctgtactta ctgcgcggta ggctgtggca tgattatgta cagcttaggc
      241 ggcgaagcaa tgaacagcaa aggaaagtta attcatattg agggtgattc tgaccatcca
      301 gtaagccgtg gggcgttatg tccgaaaggt gcaggggcgt tggactatgt taatagtgac
      361 cgccgtgtgc attatccgga agtgcgtgaa gccggttcaa gtgagtggaa acgcatttct
      421 tggcacgagg caattgaacg tattgcccgc catatgaaaa atgaccgtga tgagaacttc
      481 gtcacccaca atgaagcagg gctggaagta aaccgttgga tgacggcagg tttcttggca
      541 ggctcggcgt gtagtaacga aaccggtatc gtcacccaga agtttatccg ctctctcggt
      601 atgattttta ccgacaacca agcgagtatc tgacacggac caacggtagc aagtcttgct
      661 ccatcatttg gtcgcggtgc gatgaccaac cactgggttg atattaaaaa ctccgattta
      721 gtgatcgtta tgggcggtaa cgcagccgaa gctcaccctg tgggtttccg ttgggcagtt
      781 gaagcgaaaa aacagaacgg tgcgaagtta atggtggtag atccacgctt taaccgtagt
      841 gctgcggtgg cggatattta tataccattg cgtccgggta cggacattgc gttcttgtta
      901 ggtgcggtaa actacctgct taaaaacgac aaaattcaac acgagtatgt gaagcattac
      961 accaatgcca cattcttact caacgaaggg tttaaattcg aagacgggct tttcaccggc
     1021 tacgatgaag ccactcgtac ctataaagac aaatccactt gggcttatca gttagatgaa
     1081 cagggtcagc cgaaacggga tatgacaatg caagatccac gttgtgtgat caatatgctc
     1141 aaagaccacg tttcccgcta tacgcctgaa atggtagaac gcattaccgg tacgccacaa
     1201 aaagatttcc tgattttctg tgaagagatt gcgaaaacct cagcgccgga taaagccgcc
     1261 accttcctct atgccttagg ttggacacaa cacaccgtgg gcgcacaaaa cattcgctca
     1321 atggcgatca ttcagttgct gtgcggtaac attggtgtct ccggcggtgg ggtaaacgca
     1381 ctgcgtggtc actctaatgt acaagggatt accgacctag gcttgttccc acatatgttg
     1441 ccgggctata tccgcctgcc aaccgaagcg gacgcaacct tagaaagcta cttaaatgcg
     1501 attgcaccga aaaccttagt ggcagggcaa gtaaactacg ggcaaaacaa cccgaaattc
     1561 gctgtgagta tgctcaaaac cttctacggc gataaggcaa ccaaagagaa cgagttcggc
     1621 tatcactatt taccgaaata cccgaaaggc ggtacagatc acttccgctt tatcgaacag
     1681 atgtatcaag gcaaagtgaa cggctatttc tgccaaggta tgaacccgat tgcctcttac
     1741 cctaactcgc agaagttaat taaagcgttg agcaatttga aatatttagt catttttgac
     1801 ccgcttatta ccgatacttc cgagttctgg aaaaatcacg gtgagttcaa caatgtgaac
     1861 ccgtctgaaa ttcaaaccga agtgttccgc ttgccgacca cttgctttgc ggaagaagat
     1921 ggttcaattg cgaactccgg tcgctggtta caatggcact ggaaagcagc agacgcacca
     1981 cacgaggcaa aaccggacgt ggatattctg tctgaaatcc gtgccgaact gatccacctt
     2041 tatcataaag aaggcggaaa agcaccgctt gaaccgttag aagcgatgac gtggaactat
     2101 atgaacccgt tagaaccgaa gccggaagag atcgccaaag agaacaacgg ttatgcattg
     2161 gaagatctga aagatgcaga cggcaacatc atcgtgaaaa aaggcgaact attatccagc
     2221 tttacacaaa tgcgtgatga cggtacaacc tccggtggtt gttggatcta ctgcggtcaa
     2281 tggactccga aaggtaacca aatggcgaac cgtgataact ccgacccatc aggcttaggt
     2341 aacaccttag gctgggcatt ctcgtggccg gtaaaccgcc gtattctata caaccgtgca
     2401 tcggcagatt taagcggtaa accgtggaac ccgaaacgcc agctagttaa atggaacggt
     2461 aaaaactgga actatgtgga cgttgccgat tacggtacag caccaccaaa tagccctgtg
     2521 acaccattta ttatgcaacc ggaaggggta tccggcttgt tcgtgcgtga acgtatgcaa
     2581 gacggtccat tccctgagca ttatgaaccg tttgaaacgc caatcggcac gaacccgtta
     2641 cacccgaatg taatcagcag tccggtggca cgcattctgc cgagcgataa agaggattta
     2701 ggcaccagcg atgagttccc atatgtcggt acaacttacc gcttaaccga acacttccac
     2761 tactggacga aaaatgccct gattaacgtc attgctcaac cggagcaatt cgtggaaatc
     2821 ggcgagaaac ttgccgaaga gaaaggcatt aaaaatggcg acaaagtacg tgtttggtct
     2881 aaacgtggcg aaattaaagc cgttgccgtg gtgactaaac gtatccgttc attggtctct
     2941 gacggcaaac caatccacac tatcggtgtg ccgatccact ggggctttgc ggctacaacc
     3001 ggtgcgaaaa aaggctattt tgccaataac ttaaccgtgc gaacaggtga accaaactca
     3061 aatacacctg aatcgaaatg tatgttatta aacattgaga aaattgtggg ggcttaacaa
     3121 tgagtacagt tcaaactcaa aacattatta aaatctccgc aacctcggcg ctgacaccgg
     3181 cgccgcaggc tcgttcgcac caagtggaag tagcgaaact gatcgatgtc accacttgta
     3241 tcggctgtaa agcctgtcaa gtgggctgtt cagaatggaa tgacattcgc tccgatgtaa
     3301 atgcaaactg tgtcggcgtg tacgacaacc ctgcggatct aaatgcgaaa gcgtggacag
     3361 taatgcgctt taacgaagta gaagaaaacg accgtttaga atggttgatc cgtaaagacg
     3421 gctgtatgca ctgtgccgag ccgggttgct tgaaatcttg cccgtcaccg ggtgcgatta
     3481 tccaatacgc caacggtatt gttgatttcc aatccgacaa atgtatcggt tgtggctact
     3541 gtatcgcagg ttgtccgttc aacattcctc gtatgaatcc ggacgataac cgtgtttaca
     3601 aatgcaccct gtgtgtggat cgtgtcaatg ttggtcaaga accggcttgc gtgaaaacct
     3661 gcccgaccgg tgcaatccgc ttcggtagca aagaagagat gaagttctat gcagaacaac
     3721 gcattgctga tctgaaatct cgtggctatg aaaatgccgg cttgtacgac cctgaaggcg
     3781 ttggcggtac gcacgtaatg tatgtattac atcatgccga caaaccggag ctttattcag
     3841 gcttaccgaa agatccgaag attgatacca cagtaacctt atggaaagac gttttaaaac
     3901 ctgtggcggc agtagcgttg ggtggtttag ctttagcaga agttgcacac tatattaccg
     3961 ttgggccaaa cgttgaggaa gatcttgaag aacaccacca tagcgaaaat gcagaaggag
     4021 gcaaacatga gtaagaaatt tgaaatcacg aacgatacca aaatcgttcg tcataagccc
     4081 ttagctcgtg ttagccactg gttcttagta atctcttttt ttatgacgat gtttaccggt
     4141 gttgccttct tcttccctga ctttgcttgg cttaccgaga ttttaggtac tccacagatt
     4201 gcccgtgcag tccacccatt taccgggatt ataatgttta ttgcatttat tattatggcg
     4261 ttaatatatt ggcaccataa tatccctgag aaaaatgata ttcgttgggc aaaaggcatt
     4321 gttgaagtac taaaaggtaa tgagcataag gtttccgata atggaaaata caatttgggt
     4381 caaaaattac tcttttggac tcttattctt gcgatgttca ctcttttagc cacagggatt
     4441 attatgtgga gacaattttt ctctcactac ttctcgattc ctatgctaag aattgcaatt
     4501 ttacttcatt cagcaagtgc atttatgcta tttaccggaa ttttagtaca tatgtatatg
     4561 gcattctggg taaaaggctc aatcagaggt atggttgagg gttgggttac cgttcgctgg
     4621 gcaaaaaaac accacccaag atggtaccgt gaagaagtgc tacctgaatt agaaagacaa
     4681 gtagctgagc aaaataataa ataacctcaa gtgcggtatt attaccgcac ttttatttta
     4741 ttggtttaaa aagaatatta tgagtatcaa aatactacct caagatgata tcaaacaagc
     4801 agctagttca tttcagcaac ccgagctctt atttgctaat cccaaaaatc tgtatttacg
     4861 ccgcgctaag cggctacgtg aattagccaa gcaaaaccca ttcggtgaat atatggagtt
     4921 ttctgctaac ctcgtggaca ttcaattagc attacttgaa agccagccaa ttgcagatta
     4981 ttctcaaaaa ataaccgctt gtgtagagcg cacgcaagga gaaacaccgc taaatgcaca
     5041 gaattttcag cgtactgatg aatggcggac actactctta gccattattg agaaattcaa
     5101 gccttatgca aatgatactg ttcttgcaac aattgaagta ttggaaaagg cagctaagtc
     5161 agagctagac acacttgctg ataatttgct aaatgaacga tacgaacttg tgggcgcaga
     5221 taaagctgtc tttatttggg ctgctctctc actttactgg gtacaattag ctcaacaatt
     5281 accacgtaat tctcgtgcag aaataggcca taaacattta tgtccggtat gtaactcagc
     5341 acctgtaagc agtgtgattc atttcggtga agctcaaggg ttacgttatc tacactgttc
     5401 tttatgtgaa agtgaatggc atgtagtacg tacaaagtgt acaaactgtg atgaaaccgg
     5461 caaactggat tattggagct tagatacagt agaagcagca gttaaagctg aaagttgtgg
     5521 cgactgccat tcctacttaa aagtactcta tcaagataaa gacgtgaatg tggaacctgt
     5581 tgctgatgat ttagcaactc tattcttaga ttccgagatg gaacaaaaag gtctttcaag
     5641 aagtgggata aatccatttt tatttcaagt agagtaactc atttacaaca aaaacggagc
     5701 ataacgctcc gttttttatt acatttaact catacactat acttttttaa tcagctattt
     5761 ttttaacttc acagcggtta gcaataatct cccgatctga cataaatgcg tatcggcatt
     5821 gagatttaaa atattgaatc acagaatcat caagaggtcg ttgtgctcca acacgtaatt
     5881 cctccataga aaaattacta acctcttcat ctttctcttt atttttcaat accttacgtt
     5941 cagatattct aaacttaggc ttgtcttgct gtttttttac tccgtttaat ttttctttat
     6001 tactaggaat aggattactt gcataactaa tactattcat cgtaagaaaa agagaaataa
     6061 caaaaaagct aaaagcgttt ttcatagcaa acccataaat aaaatgtagc ataatcataa
     6121 aattacgtaa cattttatta catttagcgc atggttacaa attcttcaga tcctgtcggg
     6181 tgaatagcca ctgtgttgtc aaaatcagct ttcgttgcgc ccattttaat tgcaacagca
     6241 aaaccttgaa tcatttcatc aacaccaaaa ccaataccat gtaacccaac cactttttcc
     6301 ccttccccaa cacaaactaa tttcattcgg caaggttgac gatgttgtgt aaccgcagta
     6361 tacattgcag taaatgaaga tttatacact ttcacatttt ctgccccata ttgctcaatc
     6421 gcttttggct cacttaaacc aacagtacct attggtgggt ggctaaaaac gactgtcggt
     6481 actaagctat aatccaaatg ttcatttggt ttattattga acaaccgttc cgatagcctg
     6541 cgaccagctg caactgcaac aggagttaat tcaataccac cttcaataat atcaccaaca
     6601 gcatagatat ttggcacatt agtattttgg aatttatcca ctttgataaa accacgctcg
     6661 ttggtttcaa caccagcagc ctctaaatta atcttatctg tcgctggctc acgaccaata
     6721 gcccacacca agctttcaac agaggtttca cgaccatctt gcaattttaa tgtcaatgaa
     6781 ccatctgcat ttttaactac ttcttgagga gtcgcttgtg tatgtaattc gatcccatct
     6841 tgagctaata cttcaagcaa tgtatccact attaatgggt cttgattacg cattggagca
     6901 tgttgacgaa caaaaagatg ggtatttact cctaaactat taaatactcc agctaattca
     6961 actgcaatat accctgcccc cacaattgct acggacttcg gtaaagcatc tagtgcaaat
     7021 acaccatcag aagtgatacc gtattcagcc cctttcacat tgggtacagt tggacgacca
     7081 cctgttgcaa tcagaatatg gtctgctgtg acctcctcaa cggaaccatc agcataactc
     7141 acttctaatg ttttagcatc tttaaagcga gcaaaaccat ttaaaacatc aacattattt
     7201 tttgccagca cattaccata tgaggtatga atgcgcccaa tataggcctg gcgactctca
     7261 actaatttgg cataatcaaa attatttacc gtaatgtcaa aaccataatc aggggcgtaa
     7321 tggttaatcg cttctgcaac ttgtgcacca tagaacatca cttttttggg tacacatccc
     7381 acgttcacac aagtgccacc taaatgtttt gcttcaataa tggcacattt ttgcccatag
     7441 cttgctgctc gattaattga agcaatacca ccactaccac cgccgatagc aatataatca
     7501 taatgtttag ccataagcta ttcctcgatc tataaaaaca aaagcaactt attcgttaag
     7561 cgctttatca aattaaattt gcatagaaga ctaatgtttc atatgccctg aattcaacat
     7621 tttatcaata tacgccatcg ctaatgctga aacaataaag gctaagtgaa taactacttg
     7681 ccacatcatt tgcttttcat caagatttga agcattgata aatgtttgta ataaatgaat
     7741 agaagaaata ctgataattg ccattgaaag ttttactttc aaaatagtcg cattaacgtg
     7801 atccaaccat tctggctgat cagggtgatc atctactttt aatctagaga caaaagtttc
     7861 atatcctcca acaataacca taaccaaaag atttgcaatc atcaccacat ctattaaat
//
LOCUS       DANLUA010000114         7891 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLUA010000114 DANLUA010000000
VERSION     DANLUA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1220
                     /locus_tag="PXB44_001814"
     CDS             <1..1220
                     /locus_tag="PXB44_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5282270.1"
                     /translation="NRISEMLLNWILSPALIIYTAIIYAYVIFIAAKGQMPDGVVANV
                     ALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALLPLTLIWYAIYIRFSHYGLTEAR
                     IYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAIILISVFILDPKTVALKDQTKRLD
                     NYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEYFYSMLHYVARGMPAAQFKEKYG
                     VNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADMANAREYITFSSQLYRPNSFSHQ
                     KIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCSSITQDPTEFVIYFKGLGTTIEF
                     NINDILKKIFAKHQLSITQQHKNEVLEKIKGDLLKMDLGNSVLGLKHIELRFEDNIGY
                     IVESISTQYIMLK"
     gene            complement(1263..4292)
                     /locus_tag="PXB44_001815"
     CDS             complement(1263..4292)
                     /locus_tag="PXB44_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL5282271.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            4470..5327
                     /gene="menB"
                     /locus_tag="PXB44_001816"
     CDS             4470..5327
                     /gene="menB"
                     /locus_tag="PXB44_001816"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL5282272.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            5410..7812
                     /gene="mrcB"
                     /locus_tag="PXB44_001817"
     CDS             5410..7812
                     /gene="mrcB"
                     /locus_tag="PXB44_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL5282273.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
BASE COUNT         2331 a         1585 c         1748 g         2227 t
ORIGIN      
        1 taaaccgcat tagtgaaatg ttactaaact ggattttgtc tccggcatta attatctata
       61 ctgcaattat ttatgcttat gtaattttta ttgcggctaa ggggcaaatg ccagatgggg
      121 ttgttgcaaa cgtagcgctt ccctatttag ctttcggcct agctatacaa gcggtgcaat
      181 tattactaaa aaatgcaaaa tggcagtggt tttattctca ctttacctat ttagcacttt
      241 tgccgttaac attaatttgg tatgccatct atattcgttt cagccattat ggcttaactg
      301 aagcccgtat ttatttagtg attggcacaa taaccttatc tatttgctat accttattac
      361 tatttaaaca gttggcgcaa tatcgtctct tttctgcagt gtcgatagca attattttaa
      421 tttcagtgtt tattttagat ccgaaaacag ttgcgcttaa agatcaaacc aagcggttgg
      481 acaactattt gctaaaacac aaattattag atgtaaataa caaaatcagc acaaaagctg
      541 tacttaaata ccaagaatca ttaaatgaaa accgagatga agccgaatat ttttattcaa
      601 tgctccacta tgttgccaga ggaatgcctg ctgcacaatt taaagagaaa tatggcgtaa
      661 attcaagcta cgatttaatg agtgaaataa gagaaagaaa tactgaccca cgttattttg
      721 aagctactaa ttctgagctg gttcgcttaa ctaaagcaga tatggcaaat gctcgtgaat
      781 atattacatt tagtagccaa ttatatcggc ctaattcatt ttcccatcaa aaaatagaaa
      841 ataagctatg ccaaaaactc ctttctcaag gttactattt tgagggaaat atattaaata
      901 ttgagtatca atactcagaa acggctcaag actgctcttc aataacacaa gatcctactg
      961 aatttgttat ctactttaaa ggacttggga ctactattga atttaacatt aacgatattt
     1021 tgaaaaaaat ctttgctaaa catcagttat ctattaccca gcaacacaaa aatgaggttt
     1081 tagaaaagat taaaggcgat ttactcaaaa tggatttagg aaattcagta cttgggctta
     1141 agcatattga gttaagattt gaagacaata tcggctatat agtagaaagt attagtacac
     1201 aatatattat gctgaaataa aaggagatgc tcgttcaatt aaatttgaac gagcattttt
     1261 cgttaaaaac tcaatttaag cagccactca aaattagtct catttaaccg atattgcggt
     1321 ttcaaattcg ggccgcaaga gtttgagcca atgccgctca ttttgtaatc gacacacagc
     1381 acggtggaag ggctttctac caaatcataa ctgtggcttt tagtggttag ctcttcctgt
     1441 gtgtaaggcg aaagattaaa actaaacggc tgtggagaag taacagttat cccatttgct
     1501 tgcacataat gcgtaccgaa atggctgccg ttttcctgcg gtttgagata atcggtatga
     1561 ttttctcggg cgctggtttg ataagctccg agtttcgcca aatggtattt gtcgatataa
     1621 ctctcctgct cgccatagcc aaaatagctc acttggttat tccgtttatt caagaaaaac
     1681 cgcaagccaa agcgaggtaa aaacggcaaa tgagcggggc gggttgcctg aacctgaatc
     1741 gtcatttcgc cattcggggc aagatgataa ttcactttca gggttaaaat ccgactttga
     1801 gcggttgcca caatgccaca ttcggcagta atgtttacgc cgtttttatc ttgcatcagt
     1861 tcggtatgat acgccctggt gtaacagtta tcgtagccgg cttttttcca aaactcacga
     1921 attaagcggt cgttatcggt tggggctcgc caaatattga aatccaacgg ttgctcaatt
     1981 actgcctcgc cgtttttctc aatccggctg aaaatgcctt tgagtttatc gaaagtgtag
     2041 cgatagtcgc cctgttgaat ccgccattca tttgcaaaat cttgcacaaa aatgaccgct
     2101 tgcgggcgtg gattaagtgc tggagtgacc atcgcttgat gcagcgtgat ttggtcaaag
     2161 cctaagtgat gccctttttc caacagagcc gtgctttcat ttaaagaata atcaagggtt
     2221 aaaagttgca atttgccgtt tggttcgggc agcgttagcg gcaaacaagc ggtctgattt
     2281 ggctgacaat ttaccaaaca ttgtccctgc agggtggttt cgccgttttc gctgaaacag
     2341 taatgcacgg tcagataatc agctaagtcg ctgaaatcca actgattttt cagccaaatt
     2401 ttgcccgctt ttaactcagc tcgcaccggg cgattcacat ttttcacttc taacaaatta
     2461 gagtgtggca cacgctgttg ggaaaccaag ccgtccatac agaaattgcc atcgtgcaag
     2521 gtttcgccga aatcaccgcc atagccgtat ttttcggtct tctgttcggc acttagctca
     2581 gagttgggga gcagaggagc atggtcgcac cattcccaca caaagccacc gcacgcaccg
     2641 ctgtagcgtt ggaaggtttg ccaataatct tccatatcac cactcgaatt gcccatcgcg
     2701 tgagaatatt cacacaaaat aaaaggtttg tggttggcag agtcggaaaa atattcctct
     2761 aaccgctcgg tgcttgggta catctcgctg tataaatcga ggtttgaaag atcgttttga
     2821 tgtgccgaat gttggtaaat cgaaccttcg tagtgagtta aacggcttgg atcccgttgt
     2881 tttacccaac gggctgcgac ttcaaaattt tcaccaaagc cggattcgtt accaagtgac
     2941 caaataatga ccgaagtgcg gtttttgtct cgctccacat tggcaatgtt gcgatctaaa
     3001 atcgcctgtt tgaacagcgg atctcttgca aaataacaga aattatcgac cgcttgttgg
     3061 cgaatttctt cctgtttgtt cgggtttggc acattcagga aaatgctgtt ttccggactt
     3121 tcaatataca ctgcactgga gccgtgagat tcaagatcac tttcggcaat cagataaaaa
     3181 ccgagctgat cgcacaattc actaaaccac ggagcgttcg ggtaatgggc ggtgcgaata
     3241 gcgttgatgt tatgggcttt cattaagcgt aaatccacca acgcttgctc acggctaatg
     3301 ctgtagccgg ttttcgggtc ggaatcgtgg cggtttaccc ctttaaattt aatcggggcg
     3361 ttgttgaata gcatcacgcc attttccaca cgaatctgac gaaagccgat tttttgctca
     3421 ataatttcgt tttggtagct caagcatagc gtgtagagtg tcggatcttc cgcattccac
     3481 aaacacaaat tgcaaatttc ttggcaaaaa tcaccgcttg tttgctcgca gactaagcgg
     3541 ttttcagggt cgaaaagttg aaaatgcaca gcttcggtgt aggtggaaaa ttgtgtttcc
     3601 accttgagtt ctgcctttga aagatcagcg tttaactggt agcggataaa gaaatcttgc
     3661 aggtagtttt gctcacgttc caaaatataa acatcacgga aaatgcctga cattcggaat
     3721 ttgtcttgat cttccaaata actgccgtca caccatttta gcaccaccac gacgaggctg
     3781 tttttgccct gtttaagctg tttggtgatg tcaaattcat tggtgctgtg gctgatttgc
     3841 ccgtagccga caaattcgcc gtttacataa acaaacaggc acgaatctac gccctcaaaa
     3901 tttagcaaat agcgtttgtt aggcttgggg ttaaacgtga tttcacgctg atacaccccg
     3961 caagggtttt ggtgtggcac ataaggcgga tcaaacggaa tcgggtagtt gatgttagtg
     4021 tattgatggg catcaaagcc ttgagtttgc cagttagacg gcacggggat ttggtgggta
     4081 aatttggtgt tgaggaaatc ctccggcaga tcgtgcggac ttggatagaa attaaacttc
     4141 caatcgccat ttagcaaagt aaataactca gattgttccc gttccaactg attcggcgtt
     4201 tgccctttag caaaaggaat aaaataagcg tggtgtagcg tggtattaag gtgcaacaca
     4261 ctcgggtctt ggtagtaatt cggtaacgac ataaaagccc tatgatcaaa aagatataaa
     4321 tacgaataca ctttgattct cttataaaat agggcaatca actatgtgaa aaattgtgag
     4381 gcggatcaca aaataatttc ctcacctttt tccttcgatt ccgttataat cacaagaatt
     4441 ttttatgtca tttttcaagg aatttaccaa tgttatatcc aagcgaagaa tttttatatg
     4501 caccggttga gtgggttgat cacagcgaag gttacaccga tattcgttac cataaatcaa
     4561 aagacggtat cgctaaaatc accattaatc gtccggaagt gcgtaatgct ttccgtccgc
     4621 aaacggtaaa agagatgatt gaagcctttt ctgatgcccg ttttgatgaa aaaatcggcg
     4681 tcatcgtatt aaccggtgaa ggcgaatatg cgttctgtgc cggcggcgac caaaaagtgc
     4741 gtggcgatta cggcggctac aaagatgaaa gcggtgtgca tcacttaaat gtgttggatt
     4801 tccaacgtga tattcgtacc tgcccgaaac cggtggtggc aatggttgcc ggctatgcga
     4861 ttggtggtgg acacgtttta catatgttat gtgatttaac cattgccgct gacaatgcaa
     4921 aattcggtca aaccggcccg aaagtggggt catttgacgg cggttggggt gcaagctata
     4981 tggcacgttt agtcggtcag aaaaaagcgc gtgaaatttg gttcttatgc cgtcaatata
     5041 atgcacaaga agcgttagat atgggcttaa tcaataccgt tgtgccttat gctgatttag
     5101 aaaaagagac tgtgcgttgg tgtcgcgaaa tgctacaaaa cagcccgatt gccttacgtt
     5161 gcttaaaagc agcattgaat gcagattgtg acggtcaggc tggcctgcaa gagcttgctg
     5221 gtaatgcaac aatgttgttc tatatgacag aagaagggca agaaggacgc aatgcgttca
     5281 acgagaaacg tgagccggac ttcagcaaat tccgccgcaa tccataattg acggcaaata
     5341 caagcggttc gattttgcaa aaaatttgca aaaatcgacc gcttaccttt tctaatttaa
     5401 gttaatttta tgtcagatgt tgagttaaac gagcagagcg agcaagagcc tgagcaaaaa
     5461 ccgaaaaagc gaaactattt cctttcaacc ttattcaaaa tcggggtgat aggagcttgc
     5521 agtatggctt tttacggcat ttatttagac ggtaaaatcc gtagcaaaat ggatgggcag
     5581 atatgggaat taccagctga ggtgtatgct cgtattgaaa gcattaaaat tgaagacaac
     5641 ttaacgcttg agcaaaccaa acaagcgttg ctggataacg gctatcgtca agtttctcaa
     5701 gttgcgacac caggcgattt caaaattgaa gaaaatcagc tggtgttgct acgccgagct
     5761 ttcccgtttc cagaaacacc ggaatcgcaa cgagtgcttc gtttaacttt caaacaagat
     5821 aaattaactt atattgaaga tttggtgcag ggtaagttga tcgaggagtt ccgcttggat
     5881 ccgaaattac tggcgttact gcattccgat agcgatgaag aacgtcaagc attacgctta
     5941 caacaatacc ctcgcttgtt aattgagtca ctgattttaa ccgaagatcg ccgcttttat
     6001 cagcacgatg ggattagccc aatcggtatt gcccgtgctt taatcactaa ctaccaagct
     6061 ggcagaacag tgcagggtgg cagtacctta acgcaacagt tagtgaaaaa cttgttctta
     6121 agtaatgaaa gatcgttggt gcgtaaggta aatgaagcgt taatgtcggt gattttagac
     6181 ttccgttacg ataaaaaccg cattttagaa acctatctca acgaaattta tttagggcag
     6241 aacggtagct accaaattca cggttttgca ttggcaagcc aattctattt tggtcgtccg
     6301 atccaagaga ttagcttaga ccaaattgcg ttattggtgg gaatggtaaa aggtccatca
     6361 ctgtataacc cgtggcgaaa tccgcaaggt gcggtagagc gacgtaatgt ggtgttgcga
     6421 ttattgctgg atcatcaagt gatcaatcaa gaactttacg ctctgttaag ctcccgcccg
     6481 cttaatattc gtgaaaaagg cacgatttac cgtgagcaac ctgcctttat gcaggcatta
     6541 accattgatt tgaaaaatga gttaggcgaa attaagtcag ctcaactttc cggtactaaa
     6601 atttttacga cccttgaccg taaacagcaa cgttcagccg aacaagcagt gattaccggt
     6661 ttggaagatc tggaaagtaa aaataaaaaa gtcagcgatt tgcaatcggc tattgtcgtg
     6721 gcggaatacc gcacaggtaa agtgagggcg atagtaggag accgcttaac ccaatttgcc
     6781 ggctttaacc gtgcgattca gtcaaaacgc caaatcggtt ctttagtcaa accatcggtt
     6841 tatgcgattg ccttggcaca gcctgaatta ttccgtttaa atacaccgat ccaaaataaa
     6901 caaatttcaa ttaaggttgg ttcaacctac tggacacccc gtaactacga ccgcagtttt
     6961 agtggctcgg tgatgttaat ggacgcctta gtccgctctc tgaatattcc aacggtgaat
     7021 attggtatga aagttggctt aaaaaaagtc attgccaaac aaaaagagat gggctgggat
     7081 aatgcggaaa ttccgcctta cccatcaacc ttgctaggtt catattcgat ctcgccttac
     7141 gatgtaaccc gctcttatca agtaattgcc aacgaagggc tgaaaacacc attaaccacc
     7201 attgaggcga ttatttcgca caatggcgaa atgttctatc agcgagatag cggcaaagtg
     7261 agcgagcagg tgcttccacc ggaagctgcg gttcaaacaa tgtatgcgat gcaacaggtt
     7321 gtggaacgag gtacggctcg tagcttacaa aacgattttg ccaaactgaa attagccggc
     7381 aaaaccggta caaccaataa cgctcgagat acttggtttg tgggaattga tggcgaacac
     7441 gtcacaaccg tctggctggg taaagataac aatggggata ccaacctcac cggttcaagt
     7501 ggtgcattgt atgtgtataa gcaatatctt gagagagcct tgcctcaggc gtatagatta
     7561 ccgaaactta atacgatgca atgggtgggg attaatagct atggtagctg gaattgcgat
     7621 agtggcagac aaattccagt ttggcgagat aaagggcaac gttattgtac cagtgcaggt
     7681 aatgtagtgg ctacagaaaa accaagtgtt tgggatgcac tttcgctgaa aaaagaaaat
     7741 atcgaggcta aagcagaaga agctgaaggg aagcaaccaa tccctactga agaagttgca
     7801 ccaattgaat aaaaataaat ttgatagcca ttatcattca agatacacaa gctgatagat
     7861 tctacctata aaaagtatat attataacta t
//
LOCUS       DANLUA010000115         7874 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLUA010000115 DANLUA010000000
VERSION     DANLUA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            37..972
                     /gene="pheS"
                     /locus_tag="PXB44_001818"
     CDS             37..972
                     /gene="pheS"
                     /locus_tag="PXB44_001818"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL5282274.1"
                     /translation="MDQNLEALEEFRVEYFGKKGHFTTLMQELRNIAAEERPAIGAKI
                     NEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKTELGGLHPVSITIERVVK
                     FFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFWFDANRLLRTQTSGVQIR
                     TMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKEANFTELKGLIHDFLKAF
                     FEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSG
                     FAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1020..3407
                     /gene="pheT"
                     /locus_tag="PXB44_001819"
     CDS             1020..3407
                     /gene="pheT"
                     /locus_tag="PXB44_001819"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL5282275.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3465..3761
                     /locus_tag="PXB44_001820"
     CDS             3465..3761
                     /locus_tag="PXB44_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL5282276.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3800..4321
                     /locus_tag="PXB44_001821"
     CDS             3800..4321
                     /locus_tag="PXB44_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5282277.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4337..5380
                     /gene="degS"
                     /locus_tag="PXB44_001822"
     CDS             4337..5380
                     /gene="degS"
                     /locus_tag="PXB44_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL5282278.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5389..6063
                     /gene="cmk"
                     /locus_tag="PXB44_001823"
     CDS             5389..6063
                     /gene="cmk"
                     /locus_tag="PXB44_001823"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL5282279.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            6155..7822
                     /gene="rpsA"
                     /locus_tag="PXB44_001824"
     CDS             6155..7822
                     /gene="rpsA"
                     /locus_tag="PXB44_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL5282280.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         2459 a         1288 c         1838 g         2289 t
ORIGIN      
        1 gatttaacta ataaagcacg tgaagcttta gataagattg atcaaaattt agaggcttta
       61 gaagagttta gagtcgaata ttttggtaaa aaagggcatt ttacaacgtt aatgcaagaa
      121 ttacgcaata ttgcagcgga agaaagaccg gcaatcggag caaaaattaa tgaagcaaag
      181 caagttgtgc ttgatgtgtt aaatgccaaa aaagaagaat gggaacaagc tgtattaaat
      241 gcaaaattag ctaatgagag cattgatgtt tcactgccgg ggcgtaaaac tgaattaggt
      301 ggtttgcacc ctgtttctat cactattgag cgtgtggtga aatttttctc tgaattaggt
      361 tttaccgtag caaacggacc tgaaattgaa accgattact ataacttcga tgcgttaaac
      421 attccggatc atcatccggc tcgagcggat cacgatacgt tttggtttga tgccaatcgt
      481 ttattaagaa cccaaacctc gggtgtgcaa atccgtacaa tggaaaatgt aaaaccgcct
      541 atccgtattg tggcaccggg gcgtgtatat cgtaatgact acgaccaaac ccacacaccg
      601 atgtttcacc aaatcgaatt gttatatgtg gataaagaag caaactttac cgagttaaaa
      661 ggcttaattc acgattttct caaagcattc tttgaagaag atttacaagt gcgtttccgc
      721 ccgtcattct tcccattcac tgagccttct gcagaagtag atgtaatgcg tcaaaacggc
      781 aaatggttag aagtgttagg ttgcggtatg gtacacccga atgtgttacg caatgtaggg
      841 attgaccctg aagaatatag cggttttgct gtgggaatgg gcgtcgagcg tttaacgatg
      901 ttacgctaca atgtcacgga tttacgttca ttctttgaaa atgacttacg tttcttaaaa
      961 caatttaagt aatacaagcg gttaaaaatt tagaaaattt tacaaatagg atagaaataa
     1021 tgaaattcaa tgaatcttgg ttgcgtgaat gggtaaaccc agcaattagc acagaacagt
     1081 tatgcgacca aattacaatg ttagggttag aagttgacga tgtagagccg gtagctggtg
     1141 cgttttcggg tgtagttgtg ggtgaagtgg tggaatgcgc ccagcacccg gatgctgaca
     1201 aattgcgtgt cactaaagtg aatgttggtg gcgaacgttt attagatatt gtttgtggtg
     1261 caccaaactg ccgtcaaggt ttaaaagtgg cttgcgctgt tgaaggtgca gtgctaccgg
     1321 gcgatttcaa aattaagaaa accaaattgc gtggtcagcc ttcagagggg atgttatgtt
     1381 cttactcaga acttgggatt aaagaagatc atagcggtat tattgaatta cctgctgatg
     1441 cacctattgg tcaagatttc cgtgaatatt taaatttaaa tgatgtggcg attgaaatca
     1501 gccttacgcc aaaccgtgcc gactgcttaa gcattgccgg cattgctcgt gaagtggggg
     1561 tgattaaccg tgtcgatgtg aaagctccaa ctattacaga tgtaaaagcc acgatttcag
     1621 ataaagtttc agtggaatta caagcaccgg aggcttgccc tcgctattta gcccgtgtgg
     1681 tgaaaaatgt gaatgtcaaa gcgacatcgc cactttggtt acaagaaaaa ttacgccgtt
     1741 gtgggattcg ctcaatcgat ccaatcgttg atattactaa cttgagcctt ttagaattag
     1801 ggcagccaat gcacgccttt gatgcagcga aaattgaagg tggcattcaa gtgcgtatgg
     1861 caaaagaagg cgaagaattg gtattgctag atggcacaaa cgctaaatta caatcgaata
     1921 ccttagtgat tgctgatagc aaaggggcat tggctatggc aggtattttc ggtggcgaag
     1981 ccagcggggt gagcgagaat acgaaagatg tggtgttaga gtctgcattt tttgctccat
     2041 tagcgattac cggtcgcgct cgtcaatatg ggttacatac cgacgcatca caccgttttg
     2101 agcgtggggt tgatccagaa ttaactcgtc aagcaatgga gcgtgcaacg gcattattac
     2161 tggaaatttg cggcggtgaa gcaggtgaaa ttgtggaagc cgtgagccaa gaaaacctac
     2221 caaaacgcaa tactgtcacg cttagacgca gtaaattaga ttcggtgatt ggtcatcata
     2281 ttgacgatgc aacggtaacc gatattttaa cccgcttagg cttgaacgta acatttgcgg
     2341 ataatagttg gacggcaatt gctccaagtt ggcgttttga tattgaaatt gaagaagatt
     2401 taattgaaga aatcgcgcga atttatggct acaacagtat cccaaataat tcgccacttg
     2461 ctcatttaac gatgaaaggt acgccggaaa aattactgga agcaaaccgt attcgtatgg
     2521 cattggtaga tagtgattac caagaagtga ttacttatag ctttgttgat ccgaaaaaac
     2581 aatcattatt gcacccacag caagaagcct tgattttacc aaacccgatt tcaagtgaaa
     2641 tgtcggcaat gcgtgtttct cttttaaccg gtttgttgga cactatcgtg tataaccaaa
     2701 gccgtcagca aaaccgtgtg cgtatttttg aagcaggtct acgttttatt ccggatagca
     2761 atgcggaatc aggcgtgcgt caagaatttg tttttggtgc ggcgattgta ggcgataaac
     2821 gtccggtaca ttgggaaaac aaatcagaag cagccgattt ctttgattta aaaggcgata
     2881 ttgagcgtat tctttcttta acctctatcc gccacgatct gcaatttgtc gcaaaacagt
     2941 tcccagcatt acacccgggg caatcggctg cgattatgct tgatggcaaa gaaattggtt
     3001 ttatcggaac tgttcacccg tcaattgtgc aaaagtttgg cattaaaggc aaaccgattg
     3061 tatgtgaaat tctagcatca gctattgcag agcgtccggt tacacaagcg ggcgaaattt
     3121 ctaaatttcc tgcaaataac cgtgatattg cggtagtggt tgatgaatct gttcctgcag
     3181 gtgaggtgtt agaggcttgt cgtaaagctg gtggtaataa attagtggca gtaaatttat
     3241 ttgacgttta ccgtggttcg aatttagaaa gtggtaagaa gagtttagcc attagcttaa
     3301 cggttcaaga taatgaaaaa acattagaag aaaatgaaat caatgcggta attcaagcgg
     3361 tattagctga attagcaaca cgtttcaatg cggtacttcg tgattaatca gtaaaatatg
     3421 gctgatttat tttatcagcc ataacaataa actaaggata aatgatggcg cttaccaaga
     3481 ttgaaattgc agaaaacctg attgaaaaat ttgggttaga aaagcgtgta gcgaagcaat
     3541 ttgttgaatt gttctttgaa gagatccgat cctccctcga aaatggtgaa gaagtgaaat
     3601 tatctggttt tggtaatttt tcactgagag aaaaaaaagc tcgtccagga agaaatccta
     3661 aaacggggga aaatgtggct gtttcagccc gtcgagtagt agtttttaaa gctgggcaaa
     3721 aattacgaga acgtgttgag caagctagct cccaatcata ggggcagtta tattagttag
     3781 atttaatgtg gagtaataaa tgaaattaac caaacggtgg ctttccttag gttgtttgtt
     3841 agctattaca gcgatgttag tggcttgttc aagttcagat acaacctcta agtctcaagt
     3901 aaaaagtaaa gtttatcgtt caatatacca atcagacctt tctgatccta ttatggcaat
     3961 tagtcgatta agtgaacatc aacacgaatg gaaaggcact cgctatcgtt taggtggcaa
     4021 tagcaaggct ggcatcgact gttctggttt tatgcaagtc acttttaaag atctttttgg
     4081 gatagatttg ccgagaacca ccacagagca ggctaaggct ggtgagcgta tatccaaaga
     4141 cgaattgcgt acaggtgatt tagttttttt caagacaggg cgtggtccaa atgggaaaca
     4201 cgttggtgtt tatgtaaaaa atgggcaatt tcttcacgcc tcaacaaagg gaggggtgat
     4261 ttattctgat ttggattcgc catattggtc taaggctttc tggcaggctc gtcgtttata
     4321 atttagggta acatacatgc tgaaaaaaat cggacaagcg gtcatatttg gacttttttg
     4381 tgcaatttta attttgtttg tagctccaat tattcagagt aaagggagtt cgttttcatt
     4441 atcagcattc actcaaaaag agattgtaag ctacaatgca gcggttaaaa tagcctcccc
     4501 cgcagtcgtt aacgtatata gccgaaattt tagttctgaa aatgaaacat ttgaagttaa
     4561 gaatttaggt tcgggtgtga ttatgagtag cgatggctat attttaacta acaggcatgt
     4621 gattgaagat gcggatcaaa tcgtgattgc tttgcaaaac ggtgctattt cgactaatgt
     4681 aaaattagtc ggcgatgatt ctttaactga tttagctgtt ttaaaaattg agggtgagaa
     4741 tttacctact attccacaaa atccggagcg caaactacaa attggcgatg tggtgttggc
     4801 aattggtaat cctttgaatt taggacagag cattacgcaa gggatagtca gtgctattgg
     4861 gcgaaaaacc ttaacagaaa gtggtagaca aaattttatt cagacagatg tttccattaa
     4921 tcaagggaac tcaggtggtg ctttaattaa tactgctggt gagctaatag gtattaacac
     4981 tttgcgtttt gggaaaaata cgaatgaatt gtcagagggc ctaaattttg cgattcccat
     5041 taatctggcg aatcaaatta tgagtaagat cattaaagat ggccgagtta ttcgtggcta
     5101 ttttggggta agtacagatt tgcttgacac cgcaaaaatg caaggttatg aaaaaggggt
     5161 gatcattgtt aatgtagata aaaatgggcc agccgcaaaa gcggggattc aagcaggtga
     5221 cttagtgctt aaagtcggag atattgaggc attctctcct tctcaaatga tggaagagct
     5281 tgctgaaatg aaaccttata ctacagtaaa agtattggtt caacgtgatg aacaattact
     5341 taactttgat gtaactatta ctgagttaca gactcaatag ggtagaaaat gaaaaaaata
     5401 gtgattacag ttgatgggcc gagtggtgca ggtaaaggaa cgttatgtca tgcattggct
     5461 gaaaaattag ggtttgattt tttagattct ggagccttat accggattac tgcattaagc
     5521 gcaattaaaa aagaggttcc ccttgatgat gaggaaaaac tagcggaagt tgggcgtaat
     5581 cttgatattc aatttttgcc agaaaatggc gagattcagg tgattttgga aggagaaaat
     5641 gtaggcgact taatccgaac ggcagaagca ggtcaaaatg cctctaaagt cgctgctttc
     5701 cctttggtac gtgaagcact tttagaacgc caaagagcct ttagtagcga aaaaggtctg
     5761 attgcagatg gacgagatat ggggacagtt gtgtttcctg atgcacaaat taagctgttt
     5821 ttagatgcaa gtgccgaaga acgcacaaaa agacgtgtaa aacagttgca agaaaaggga
     5881 tttaatgcta actttgacga gattttagcc gagataaaag aacgtgattt tcgcgataga
     5941 aatcgatcag tcgcgccact tgttccggct aaagatgcgt tgctgttaga ttcaacgcat
     6001 ttatcaattg aagatgtgat taaccaagca ttaaatcata tcgctcaata cattaaattt
     6061 tagtttgttg cttcacatag gatttgtgta gcttttatta tcaaccccgt ttaacaggac
     6121 gttagatgga tgtttatttt taagaagatt tattatgtca gaattatcgt ttgctcaact
     6181 acttgaagaa tcatttgcag ctacaccacg tttaggtgat gttgttaaag gtactgttgt
     6241 tgctatccaa aaaggcttag ttattgttga tacaggtttc aaatctgaat ctgcaatcga
     6301 tgcctcagaa tttactaacg cacaaggtga attagaagtt caagtgggtg atgaagtaga
     6361 tgttgttctt aaagctgtag aagacggttt tggtgcaaca gtagtttctc gtggtgatgc
     6421 aaaacgtaac gaagcttgga ttgctttaga gaaagcattt gaagaacaag ctacagttat
     6481 cggtttcgta aatggtaaag taaaaggcgg tttcactgtt gaattaaacg gtgttcgtgc
     6541 attcttacct ggttcattag ttgatactcg tccggttcgt gattctttaa acctagaagg
     6601 taaagagtac gaattcaaag taatcaaatt agatcaaaaa cgtaacaacg tagtagtatc
     6661 tcgtcgtgct gttatcgaaa cagaaagcaa ccaagatcgt gatgcggtat tagcaaactt
     6721 agttgaaggt tctgaagtta aaggtacagt taaaaactta actgactacg gtgcattcgt
     6781 agatttaggt ggcgttgacg gtttattaca tatcacagat atggcttgga aacgtgttaa
     6841 acacccaagt gaagtggtta atgtaggcga tgaagttact gttaaagtat taaaatttga
     6901 caaagacaaa actcgtgtat cattaggctt aaaacaatta ggtcaagatc cttgggctgt
     6961 aattgctgaa aaccacccgg taaacagcaa attaactggt aaagtaacta acttaactga
     7021 ctacggttgc ttcgttgaga ttttagacgg tgttgaaggt ttagttcacg tttcagaaat
     7081 ggattggaca aacaaaaaca ttcacccatc taaagtggtt aacgtaggcg atgtagttga
     7141 agtaatggta cttgaagttg atgaagaacg tcgtcgtatc tcattaggtt taaaacaatg
     7201 caaagcaaac ccttgggaac aatttgcggc aactcacagc aaaaatgata aagtatcagg
     7261 taaaatcaaa tcaattactg acttcggtat tttcatcggt ttagaaggtg gtattgacgg
     7321 tttagttcac ttatctgaca tttcttggaa tgtttcaggt gaagaagcag ttcgtaacta
     7381 caaaaaaggt gatgaagtag aagctgttgt tctacaagtt gatgcagtga aagagcgtat
     7441 ctctttaggt atcaaacaat tagaatctga tcctttcaca aactttgtag atggtactaa
     7501 aaaaggtgca atcttaacag gtaaagttgt agaagttgat gctaaaggcg ttaaagttga
     7561 attggaaggt ggcgttgaag catttattcg tgcaaacgaa gcaactgctg agcgtgtaga
     7621 agacatcact tctgttattt ctgttggcga ttcaatcgaa gcgaaataca ccggtgttga
     7681 tcgtaaatct cgcgtagtta acttatctgt tcgtgcaaaa aacgaagctg aagaatcagc
     7741 agcaattgca caagtgaaca aagaggaagt tgttgtacct aatgcatttg ctgaagcttt
     7801 caaagcagct aaaggcgaat aattagtata gactaatcta aaatgggtat gttttgaaca
     7861 tacccattta aact
//
LOCUS       DANLUA010000116         7852 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLUA010000116 DANLUA010000000
VERSION     DANLUA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            57..1484
                     /gene="hldE"
                     /locus_tag="PXB44_001825"
     CDS             57..1484
                     /gene="hldE"
                     /locus_tag="PXB44_001825"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL5282281.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            1498..2052
                     /gene="pyrR"
                     /locus_tag="PXB44_001826"
     CDS             1498..2052
                     /gene="pyrR"
                     /locus_tag="PXB44_001826"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL5282282.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            2125..3075
                     /locus_tag="PXB44_001827"
     CDS             2125..3075
                     /locus_tag="PXB44_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5282283.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            3137..4003
                     /gene="rsmA"
                     /locus_tag="PXB44_001828"
     CDS             3137..4003
                     /gene="rsmA"
                     /locus_tag="PXB44_001828"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL5282284.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4114..4743)
                     /gene="upp"
                     /locus_tag="PXB44_001829"
     CDS             complement(4114..4743)
                     /gene="upp"
                     /locus_tag="PXB44_001829"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL5282285.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(4768..6453)
                     /gene="fadD"
                     /locus_tag="PXB44_001830"
     CDS             complement(4768..6453)
                     /gene="fadD"
                     /locus_tag="PXB44_001830"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL5282286.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(6431..6895)
                     /locus_tag="PXB44_001831"
     CDS             complement(6431..6895)
                     /locus_tag="PXB44_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL5282287.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(6892..7845)
                     /locus_tag="PXB44_001832"
     CDS             complement(6892..7845)
                     /locus_tag="PXB44_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL5282288.1"
                     /translation="MFWVISACYTFAWAESTIKTPLAYLTAMSEAHKKSTYELVYILQ
                     QGSDTESFRLRHAFTHNKEYAQLLNLDHSREEIILKNNKVSYLGYNFRPFSLNSSQIL
                     DNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIRMKPNDTLRYAYILWIDEETHLL
                     LKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLMLPTLTTVKKEEFPKSDGWQV
                     KWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTLTIYIMPSQGVSFNEYAWQQ
                     GKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP"
BASE COUNT         2323 a         1699 c         1550 g         2280 t
ORIGIN      
        1 ctaataatgg cacaagcgtg gacgcttgcg ccatcattta acagagaata ttaactatga
       61 tgatgcaata ctctccaaaa tttaataatg ctaaagtgct ggtgcttggc gatgtaatgt
      121 tagaccgtta ctggttcggg gcaaccaatc gaatttcgcc tgaagcccct gtgccggtgg
      181 tgaaagtaca agatattgaa gagcgtgccg gcggtgcggc aaacgtggcg atgaacatcg
      241 ccagccttgg cgtaccggta acattacacg gcttaattgg cgatgatgat gccgggcgtg
      301 ctttagataa attacttaat tcccaccaga tccaaaatca ttgtgtggtg attaattcgc
      361 acccaaccat cacaaaattg cggattcttt cccgccatca gcaattattg cggttggatt
      421 ttgaagaggg ttttcacaat gtagattcta ccgccctact tgcaaaactt tcgcaagaaa
      481 ttaccgctta cggagcatta attctctcgg attacggcaa aggcacattg gaatcggtgc
      541 aacaaatgat ccaaattgcc cgcaatgcta aggtgccggt gctgatcgac ccgaaaggca
      601 cagactttga acgctatcgt ggggcaacat tgcttacacc aaatatgtcg gaatttgaag
      661 cggtggttgg gcattgtaaa gatgaggatg agattgtcag caaagggtta aaactgattg
      721 ccgactatga tttatctgca ctattagtta cccgttcgga aaaaggaatg accttacttc
      781 gcccgaacca cgagccttat catctgccga cccaagccaa agaggtttat gatgtaaccg
      841 gtgcaggcga taccgtaatc agtgtattag ccactgcgat tgccgatggc agagcctatg
      901 aagaagcctg ctatttagcc aatgctgccg ccggtgtagt cgtaggtaaa ttagggactt
      961 caaccgtcag cccggttgag ctggaaaatg cgattcacca acgcactgcc accggttttg
     1021 gcgtggtttc tgaagccgaa ttaaaagcga ttgtggaaga agcgaaaaaa cgtggcgaaa
     1081 aaatcgtgat gacgaacggt tgctttgata ttcttcaccc cggtcacgtt tcctatttgg
     1141 aaaacgcccg taaattaggc gaccgattaa ttgtagccgt caataccgat gcttcggtaa
     1201 aacgcctaaa aggcgaagct cgcccgatta atgatttagc tgcccgtatg gcagttttag
     1261 ctggtttagc ctctgtagat tgggttgtgc cttttgatga agatacacca caacgtttaa
     1321 tcagtgaaat cctacctgat ttattggtaa aaggtggtga ttacaagccg gaagagattg
     1381 ccggcagcca agaagtatgg gcaaacggcg gcgatgtgcg agtattgaac tttgaaaacg
     1441 gctgttctac cactaatgtg atcaagaaaa ttcaagcact ataatggtaa taagcgaatg
     1501 gcgagagaaa aaatcattat tgataccgag caatttcagc gcacaatatc tcgtatttca
     1561 catcaaatta ttgaaaaaca cggtgatttg caagatgtag tgattgtcgg catcaaacgt
     1621 cgaggagctg aaattgccga gttaatccaa aaacgcattg aagatttagc tcaaacacgc
     1681 ttgccttata tggcgttaga tattacgttc taccgtgatg acctagactt aatttatcaa
     1741 gatcccgtat ttatgggagc cgataatcag ctaaatctga acggtaaaaa agtgattttg
     1801 gttgatgatg ttctgtttac cggcagaacc attcgggcgg cacttgatgc actcttagat
     1861 tttggcagag ctgccagaat tgaactggtg atttttgtcg atagaggtca tcgggaattg
     1921 cctatccgag ccgattatgt cggaaaaaat attccaacgg ctaaaaacga gcaaattcag
     1981 gtgcaaacgc tctcgtatga tgggattaac caagtggtct tagttcctgc tgccaataaa
     2041 gaaagtgctt aaattaagtg aaccttttcg ctaaacttat ctctaaggaa aagttactgc
     2101 ttaattacct ccattaggat tgttataaaa atgaaattag tcaaatcatt atttgctgtt
     2161 gccgttgcaa caatgagtct tacccaaacc gcccacgcat ttgaagaaag agtggtggcg
     2221 ttagtgaatg atacccctat tatgcaaagc caggttcaac gtgtgcttgg caagaaaaag
     2281 gcgacagaaa ctgctcaacg tgcggctatt gatcaagtga ttgatgatat gttggtgcaa
     2341 caggcaatga aagaagccgg cgttaaagtc agcccagctg cggtaaacca agcggttgaa
     2401 aatgtagcaa ttcaaaacgg cattacttac ggtcagttat tagatgcgtt agattatcaa
     2461 ggtattacac ttgaacaatt caaacgtaat attgctcaac aaatggcaat ggaacaagtt
     2521 cgccatatca gcattagcaa atcgattcag gttgcaccac agcaagtgca agctctggcg
     2581 aaagagttaa tggcgaaaga taaagcagca ggcaaattga aaaccgtttc tggcaaagag
     2641 catcgcatta gtcatatttt gctcaaaacc aacccgattt taaatgatgc tcaagccaaa
     2701 gcaaaactta gcagcttaac tactgatatt aactcaggca aaatcagctt tgaagaagcg
     2761 gcaaaaaccc attctgttga ttatgcctca ggggcagacg gtggagattt aggttttaac
     2821 ttcttagaaa tttacgatcc ggcatttgcg aatgttgcat caaaaatcaa accaaaccaa
     2881 atttctgctc cgtttaaatc gcaatttggt tggcatattt taaaagtcac cgacactcgt
     2941 aacggagaca gaaccgaaga tgcttaccac caacgtgctt atcagcaact ggtgaacaag
     3001 caagccgaag aagcctctaa agattgggta aaagcgctta gaaaaaccgc caatattcaa
     3061 tatgtcggtc aataattaaa atttttaaga acctcgcata atgcggggtt cttttcttat
     3121 ttcaaaaagg ataacaatga gttctacaaa ttccaaaaaa catttaggtc ataccgctcg
     3181 taagcgtttc gggcaaaact ttttgcacga tatgaacatt attcacagca tagtttcagc
     3241 cattaacccg aaaaacggac aatttttatt agaaatcggt ccgggtttag gagcattaac
     3301 cgagccggtt gccgaattgg tagataaact cacggtggtt gaaattgacc gtgatttagc
     3361 agagcgttta cgccaccacc cgtttttaaa ccaaaaactc accattattg agcaagatgc
     3421 cctacgcttt aatttccgtg actattttaa ccaactcaac ttagacgaag actcggtgcg
     3481 tgtgtttggt aacttacctt acaacatttc tacgcccttg atattccacc tgctgacctt
     3541 tcacgactta attcaagata tgcactttat gttgcaaaaa gaggtggtga aacgtttatg
     3601 tgccgcgcca aacagtaaag catacgggcg tttaaccatt atggctcaat actattgcca
     3661 agtaattccg gtgctggaag tgccgccaac cgcctttaaa ccggcaccaa aagtcgattc
     3721 tgcggtagtg cgtttagtgc cttataacca actgccttat ccggcaaaag atgtgtattg
     3781 gctcaatcgg gtcacgactc aagcctttaa ccaacgccgt aaaaccttac gcaatgctct
     3841 ttcaaccctg tttagtgcgg agcaactgga cgccttaggc gttgatttaa ccgcccgagc
     3901 ggaaaacctt accattgccg attatgtgcg tttggctaac tggctacacg acaacccgcc
     3961 ggcaattgat acaaccgaag aattggttga tgaagattgc taatcaataa attacaagcg
     4021 gtcatttttc taccaagatt tgcaaatctt tagtgaaaaa tggccgcttg tttaaatcag
     4081 ctttacttgt tttgctgtta cttagttttg atgttacttg gtgccgaaaa ttttatcccc
     4141 tgcatcgcct aagcctggaa tgatatagcc gttttcgttt aagtgactat cgattgaggc
     4201 ggtgtagagt tcaacatctg ggtgggcggc ttctaatact ttaatcccct ctggagcggc
     4261 aactaacact agcactttaa tctgcttaca gcctgctttt ttcaggaggt caatggttgc
     4321 taccattgag ccaccggttg caagcattgg gtcggtgata atggctaaac gctcatcaac
     4381 atcgttggct agtttggaga aatacggcac gggctttaag gtttcttcgt cacgataaat
     4441 cccaaccaca ctaattctgg cacttggaat atgctctaac acaccgtcca tcatacctaa
     4501 gccagcacgc agaatcggca caacagtcac ttttttgcct ttaattcgct caaccgcaat
     4561 tttctctccg ctccagccat tgatctccac actttccatt tccagatctt tggttgcttc
     4621 gtaagtaagc agactaccaa tttcattcgc tagctcacgg aaatctttgg tgctgatttc
     4681 cgcttcacgc attaagccca atttatgctt tacaagtggg tgttttactt caacaatttt
     4741 cattttaagc tccttaatta gtgtaattta agccttattc acctttgcta attcttcgtc
     4801 tcttaacact cggcgaagaa ttttgccgac attacttttt ggtaattcgt cacggaattc
     4861 gatgtctctt ggaattttgt agccggttaa gaattgacga caataggttc tcaattcatc
     4921 tcgagttaaa ctttcatctt ttttggttac aaaaatttta atgctttcgc cggaagcctc
     4981 actcggcaca ccaatcgcca ctacttcatt cacttttggg ttttgggaaa tcacttcttc
     5041 aatttcggtt gggtacacat taaaaccgga gacaataatc atatcttttt tacgatcaac
     5101 aatgcgtaag ttcagatcat cccccattac cacaatgtca ccggttgcca tccaaccatc
     5161 ttgtagcact tgtgccgttt catcaggacg gttccaatag ccttgcatta cttgcggccc
     5221 tttcaccacc agctcgcccc gttcgccgag tggtacttct ttgccttcat catccacaat
     5281 acgaatgtca gtatttggca ctggcacgcc aatagagccg gaatattcca ccgaatcgcc
     5341 acgggttgca ctaatcagcg gagaacattc tgtcattcca taaccttcga taatatgtac
     5401 gccggttaaa tcgtgccagc gttttgccac gctagattgg atcgctgcac cgccacctac
     5461 acttaatttt aggtgtgaaa aatcaacctc tttaaaactt tcgtgattaa gtaatgcatt
     5521 aaacaatgta tttaccccag taatcactga aactttgtac ttttttaatt ctttaataaa
     5581 gcctggaata tcgcgcggat tagtaattaa taatccggta agtcctaatt cgataaagag
     5641 caagcaattc atcgaaagtg ctaagacgtg atacagcggt aaaggtacca atccaatcat
     5701 attggcagaa ttacccaaca atggctcaac aatccatttt gcctgcatca tattagcaat
     5761 taagttttga tgagtcagca ttgccccttt cgctacacct gtagtaccgc cagtgtattg
     5821 taaaaaggcg agatcttggc tgacaatatt cggttttaca tattgacgct gtttgccaat
     5881 gcttaaggct tcacggaagc tgaccgcatg tggcaattta tatttcggca ctaatttttt
     5941 gatgtatttc accacaaaat tgactaaggt gcgtttgccg aatgaaagtt ggtcgcccat
     6001 tcgggtcaaa ataacgtgct taatctcagt attaaagaca attttttcta aggtagccgc
     6061 gaagttcgag actatcacaa tcgctttcgc accactgtca tttaattgat gttctaattc
     6121 acgcggcgta taaagcgggt tgacattcac caccacaagc ccggcacgta atgcgccaaa
     6181 taaagcgatc ggatattgca ataaattcgg catcatcaat gcgattcgat cacctttttt
     6241 caggcgtaat tcattttgca aataagcggc aaacgagcgg ctacgttctt ccaatttacg
     6301 gaacgttaaa acctgcccca tattaatata agctggaata tcagggtggc gttgtatcgc
     6361 tgcttcaaac atttccacta aagattcata ttgatctgtt tcaatcgttt ttggtgaata
     6421 aggcggataa ttatttagcc atattttttc cattatagtc tcctaaaacc ttacctaaaa
     6481 agataggtga aaaatttgct tgtttgctgt gtttatgaat caattttctt acagcccaga
     6541 atgttatacc tgttgaaagc aacattataa ataaaacaat aagctcattt gcaaaaaatt
     6601 gcgaaaatcc gaccgctgtc atcacaagaa ccagcagagg aatgccataa attaaaaaca
     6661 cgcttttgag caagatattt tcagctaacc ctagttcaac cttatcgcct acttttaggg
     6721 gttcatctac ctcaatacga aaacgagggt caattttttc acctgctaat gctgataatg
     6781 ctttgctgcc acaagcagac tgagctacac agccaccaca gctactttta gcataacatt
     6841 gtacaatagc cacaccattc tgatattcca ccaccgtagc ctgttcgatc atcatggctg
     6901 agctccttcc ttaaaaccaa tattgttcat aatttgctta gccgattgca acggcacatc
     6961 accaataatc acaatatctc tatcattaat cgtttgattt aaaatcgtta atttaccttg
     7021 ttgccaagcg tactcattaa aactcacgcc ttggctaggc ataatataaa tagttaatgt
     7081 agataaacca tcactatata ctctgctatc tacatactca ctttccactt gatatgtcgc
     7141 acctgtgact gtttgttgct gaattaacct aaaccctgtc ggcaaccact tcacctgcca
     7201 accatcagac ttaggaaatt cttctttttt cactgtcgtt aatgttggta acattagtga
     7261 ttctattaca tttgcaatcc tatccaattc tggagattga taaagttgca aaactctgaa
     7321 ttcttctaat accaaattat ccatattcaa gacttggctt ttaagtaata aatgtgtttc
     7381 ttcatcaatc catagtatat aggcatatct taaagtatca tttggcttca tacgaattac
     7441 ctttgtaatt cggtcagaaa tacgatcttt accagcatct aaaaaggcat aaccttttaa
     7501 atttgcataa tctgtgtaaa gtacattagg gaggttatct aaaatctgtg aactatttaa
     7561 actaaaagga cgaaaattat accccaaata gctgaccttg ttatttttta aaataatttc
     7621 ttctcggctg tgatctaaat tcagtaattg ggcatattct ttattatggg taaaagcatg
     7681 acgtaaacgg aaagattctg tatcacttcc ctgttgaaga atataaacta attcataagt
     7741 ggatttttta tgtgcttctg acatagccgt taaatatgct aatggtgttt taatagttga
     7801 ttctgcccaa gcaaatgtat agcaggcaga aataacccaa aacaacaata ag
//
LOCUS       DANLUA010000117         7747 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLUA010000117 DANLUA010000000
VERSION     DANLUA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            108..1316
                     /locus_tag="PXB44_001833"
     CDS             108..1316
                     /locus_tag="PXB44_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL5282289.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1369..3504
                     /gene="pta"
                     /locus_tag="PXB44_001834"
     CDS             1369..3504
                     /gene="pta"
                     /locus_tag="PXB44_001834"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL5282290.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3618..4298
                     /gene="can"
                     /locus_tag="PXB44_001835"
     CDS             3618..4298
                     /gene="can"
                     /locus_tag="PXB44_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL5282291.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLNDEN"
     gene            4622..6343
                     /locus_tag="PXB44_001836"
     CDS             4622..6343
                     /locus_tag="PXB44_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL5282292.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
     gene            6336..6827
                     /gene="ilvN"
                     /locus_tag="PXB44_001837"
     CDS             6336..6827
                     /gene="ilvN"
                     /locus_tag="PXB44_001837"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL5282293.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(6906..7742)
                     /locus_tag="PXB44_001838"
     CDS             complement(6906..7742)
                     /locus_tag="PXB44_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5282294.1"
                     /translation="MLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNNIG
                     FFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSGLF
                     LKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLSRY
                     DSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAVAK
                     AYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAETKQ
                     SN"
BASE COUNT         2471 a         1429 c         1590 g         2257 t
ORIGIN      
        1 tcaagcattt attttaagat ctgaaaggtg tatcctttac accttaaata caccaataaa
       61 aatagtattt tcttcaaact catttcataa aataaggtaa tttgaccatg tctaaaaact
      121 taatcttaat ccttaactgc ggtagttcat ctcttaaatt tgctatttta gatcctaaaa
      181 caggtgatga aaaattatct ggacttgctg aagcttttca tttagaagat gcacgtatca
      241 agtggaagtt aaacggggag aaaggtaatg ctgatttagg tgcaggtgcc gcacatagtg
      301 aagcacttac ctttattgct gaaaaattgt tatctgacga attaaaagca ggtatcggcg
      361 cgattggtca tcgtattgtt catggcggtg aaaaattcac ctcatcagtt gtaattaccg
      421 atgaagtgat taaaggtatt gaagatgcaa cgcaatttgc accattacac aaccctgcgc
      481 atttaattgg tattaaagaa gcattccgca ttttccctga attaaaagac aaaaacgtag
      541 ctgtatttga cactgcattc catcagacaa tgccggaaca cgcattctta tatgcacttc
      601 catatacatt ctataaagaa aatggtattc gtcgctacgg tttccatggc acaagccact
      661 tctatatcac ctcacaagtt gcagaattag caggtaagtc tgttgagcaa accaatgcca
      721 ttatttgcca cttaggtaac ggtggttctg tttctgttgt acgcaatggt aaatgtattg
      781 atacctcaat gggtttaact ccattagaag gtttagtaat gggtactcgc tctggtgact
      841 tagaccctgc gattatctct ttcttataca ataacttagg tatgtcaatg gacgaaattg
      901 atactactct agtaaaaaaa tctggtcttt taggtttaac tggcgtaact agtgactgtc
      961 gttatgcgga agataattac aatgacgtat caaaaccgga agcaaaacgt gctttagatg
     1021 tatatagcta tcgtttagct aaatatattg gttcatatat ggcaatttta ggcgatgacc
     1081 acttagatgc tatcgcattt acaggtggta tcggtgaaaa ttcaggctta gtacgtgaat
     1141 taaccttaaa tcacttaaaa ttatttggca ttaagttaga tcaagagcgt aaccttgctg
     1201 cccgttttgg taaagatgga gtcattacag cggacgagtc aacatttaaa gctgttgtta
     1261 tcccaactaa tgaagaactc gtgattgcac aagataccgc taaattagca ttataatcat
     1321 agggggcaga tattctgccc tttcactttc ttttaatttg gaaatattat gtctagaacg
     1381 atcattctta tccctatttc aactggtgtt ggtttaacca gtgttagctt aggtttagta
     1441 catgctttgg agcaaaaagg ttctaaagta ggatttttaa aaccgatttc tcaacctatc
     1501 agcggtgaag atacgttaga ccgctcaaca acgattatca gagcagaaca aacaacagag
     1561 gttggtgagc cgtttatgtt aagcgaagct gaaacattaa tcggtcaaaa ccaaacggat
     1621 gtattgttag aaaaagtggt ggaacgccac caaaaattat caaaaaataa tgaaattgtc
     1681 attgttgaag gtttaattcc aacgcgtaaa aactcatacg ctaacagcat taactatgaa
     1741 atggctcaag cccttgatgc tgaaattgta ttagttgcag caccggcctc agatacacca
     1801 gctcaattaa aagagcgagt tgaagctgcc gcttcacaat ttggtggacg taacaaccca
     1861 aatttactgg gcgttattat caataaattc aatgcaccag tagatgattc aggtagaacc
     1921 cgtccagatt tgactgaaat ttttgattca tttcaacact caaataataa tgtttcagaa
     1981 gtcgagcatc tttttgctaa aagtccaatt aagctgttag gctgtattag ctggaaagca
     2041 gaacttattg ctacacgagc aattgatgtt gcaaaacatt taggcgcttc aattattaat
     2101 gaaggcgaaa taaaggctcg tcggattcgc ggtgtgacat tctgtgcacg taacttacca
     2161 aatatggtcg atcattttaa agccggtagt ttattagtta cctctgcgga tcgtcctgaa
     2221 gtgttagttg ctgcatcgtt ggcggtaatg aatggcatcg aaattggtgc tattttacta
     2281 accggtggtt ataaaattga tagctcaatt gcaaaacttt gccaacaagc atttgaaacc
     2341 ggtgtgcctg tattccgtat tgaaggtaac acttggcaaa ccgcattaag cctacaaagc
     2401 ttcagcttag aagtgcctgt agatgataaa gagcgtattg ctgcaattaa agaatatatg
     2461 gcaagcaaat ttaatgtagg tttcattgat gaggtttcta aagccgctac tcgtgcaaga
     2521 cgtttatcac ctgcagcatt ccgttaccaa ttaaccgaat atgcccgtca agcgaaaaaa
     2581 cgcattgttt tacctgaagg agatgagcct cgtactgtga aagcggctgc attatgtgct
     2641 gagcgtggca ttgcagaatg tgtgttatta gctaacccaa cagacgtaca acgtgttgct
     2701 gaagcacaag gcgtggtatt aggtaaaggc gtaacgatta ttgaccctgc gagcattcgt
     2761 gaaaattatg ttgatcgctt agttgaattg cgtaaagcaa aaggaatgac tgaagttgtt
     2821 gctcgtgagc aacttttaga tacagttgta ttgggtacaa tgatgcttga agctaatgaa
     2881 gtggacggtt tagtctcagg cgcagttcac acaaccgcta acactattcg cccaccaatg
     2941 cagatcatta aaactgcacc gggaagctct attgtatctt ctatcttctt tatgctacta
     3001 cctgatcaag tattggtata tggtgactgt gcggtaaacc ctgacccaac tgcagaacag
     3061 ctagcggaaa ttgcgattca atcagcagaa tctgccaaag cctttggtat tgaccctcgt
     3121 gttgcaatga tctcctactc taccggtaca tcaggttcag gtgcggatgt agaaaaagtg
     3181 aaagaagcaa ctcgtattgc ccaagaaaaa cgtccagatt taatcattga tggcccatta
     3241 caatatgatg cggcgattat ggaagatgtt gcccgctcaa aagcaccaaa ttcacctgta
     3301 gcaggtaaag caactgtatt tgtcttcccg gatttaaata ctggtaatac cacctataaa
     3361 gcggtacaac gttcggcaga tttagtctct atcggcccaa tgttacaagg tatgcgtaaa
     3421 cctgtaaatg acttgtcccg tggggcttta gttgacgaca tcgtttatac tattgcgtta
     3481 actgcgattc aagcaacgca ataattccct cccaaatgaa accaaatgcc cttaaaacta
     3541 agggcatttt tattttttac ctacccgact tttcaattta atcatataat caagattagt
     3601 gttaaaaagg agaatttatg gaaaaaattg aacgcttatt tgccaataac cacgcctggg
     3661 caactaagat gaaagatgaa caatcggatt attttaaaga attagcggaa catcaaaagc
     3721 caacctattt atggattggc tgttccgata gtcgtgtgcc tgctgaaaaa ctcacagggt
     3781 taggccccgg agaattattt gttcatcgaa atgtagcaaa tatggtgatt cacactgatc
     3841 ttaattgtct gtcggttgtt caatatgcgg tagatgtatt agaaattgag catattatta
     3901 tctgtggtca cactaattgt ggtggtatca atgcggcaat tgcacaaaaa gaacttggtt
     3961 taattaataa ctggttgtta catattcgtg atatctggtt taaacacagc catttactag
     4021 gcaaacttcc ctatgaagag cgatcaaatg tcttgacccg attaaatgtt gcagaacaag
     4081 tttataattt aggtcagtcc tctattatta aaaccgcttg ggaacagggt aaatccctct
     4141 cgctacacgg ttgggtatac gatgttacag atggtttctt gattgaccaa ggtgttatgg
     4201 ctactagccg agaaacatta gaaattacct atcgtaatgc aatagctaaa ataacaaccg
     4261 aagcaaacga aaaaatagtt ttaaacgatg aaaattagcc attgtaagcg gttaaattta
     4321 tcgatttttt tacaatcttt ttataaaaaa ccattgtttg atggtttttt attttcctga
     4381 ttcctgttat cgtcaaaaaa gttttaaatt tcagcaaaag tgtaaaatat tgatttaaaa
     4441 atcaaaaaca tacaaatata tagattttat ttttataaaa tcccaacttt ttaagataaa
     4501 aaagaatata aatctagcaa atctagcatt tgatcccata ataagaacta gacatcaata
     4561 aaattttact gcaaaatcca accatcttag agaagaaaat gctattttgt tggaggattc
     4621 aatgaaaaaa ctttctggtg cagaaatgat tgtacagtcc ttaaaggatg aaggcgtaga
     4681 atacgttttc ggctatccgg gtggctctgt actcgatatt tatgatgcaa ttcatacact
     4741 tggattgaat cacgttttag ttcgacacga gcaaggtgct gtacatatgg ctgatggcta
     4801 tgcccgttca acaggtaaag tcggctgtgt gctagtgact tcaggtccgg gagcaacgaa
     4861 tacaattact ggtattttaa ctgcttatac cgactctgtt ccgttagtaa tactgtcggg
     4921 ccaagttcca tcaagtttaa ttgggacaga tgcattccaa gaaagtgata tgatcggcat
     4981 ttctcgtccg gttgtaaaac acagtttctt agtaaaaaat acagaagaaa ttcctgaaat
     5041 tatcaaaaaa gccttttata ttgcatcaac cggtcgacca ggcccggtag ttattgatat
     5101 tcctaaagat gtagtcaatc cattaaataa atatccctat gaatatccgc aagaagtatc
     5161 aatgcgttct tataacccaa cagttcaagg gcataaaggg caaattaaaa aagcattaaa
     5221 agcactatta gtagcaaaac gtccagtgct ttatgtaggc ggtggtgtaa ttagtgcaga
     5281 gggttcgcca gaaattacag agtttgcaca aaagctaaat ctacctgtaa cctgttcatt
     5341 aatgggatta ggtgcttatc ctgcctctga taagcaatat ttaggtatgt taggcatgca
     5401 cggcactttt gaggcaaata atgcaatgca cgaaagtgat ttgattttag gtattggggt
     5461 acgttttgat gatcgtacaa caaataactt agcaaaatat tgcccgaatg caaaagtgat
     5521 ccacgtggat attgatccag cctcaatttc caaaacagta caagcttata ttccaattgt
     5581 aggcagtgct aaggatgtaa tggaagaatt tttatcttta ttaggtgagg aaaatcttgc
     5641 aaaaaatcag gtagatttaa ccgcttggtg gcaacaaatt gatgcttgga aagctcgtaa
     5701 ttgcttagcc tttgaagaaa acagtgatgt gattaagcca caacaagtga ttagaatgat
     5761 ccacaagttg accaatgggg aggcttatgt tgcctcagat gttggacaac atcaaatgtt
     5821 tgctgcattg cattatgggt tcgataaacc acgtcgttgg attaattcag gcggtgcagg
     5881 cacaatgggc tttggcttac ctgctgcgat gggggtaaaa tttgcccacc ctgatgccac
     5941 tgttgtttgt gtaaccggtg atggtagtat ccaaatgaat atccaggaac tttccaccgc
     6001 aaagcaatac gacacaccga ttgtgattgt cagcttaaat aaccgtttct taggtatggt
     6061 gaaacagtgg caagatatta tttactctgg ccgccattca caggtgtata tgaattctct
     6121 acctaacttt gctaaattag cagaggctta tggccacgtt gggattcaaa tcgaccaccc
     6181 ttctgaattg gaggcaaaat taactgaggc attctcaatt aaagataagc ttgtatttgt
     6241 tgatgtattg gtcgatgaaa cggaacatgt ttacccaatg caagtgcgtg gaggagcaat
     6301 gaatgaaatg atgttaagta aaacggagag aacagatgcg tagaacatta gccgtattat
     6361 tagaaaatga atcaggtgca ttatctcgtg tagttgccct tttctcgcag cgtggtttta
     6421 acattgaaag cttgaccgtt gcccctactg atgatccaag cctttcccgt atgacaattg
     6481 tcgcgcaagg cgaagaacag gtgttagaac aaatcgaaaa gcaactacac aaactcattg
     6541 atgtgtttaa agtgagtaac ttaagtgctt atgaacacgt tgagcgtgaa gtgttattgg
     6601 taaaagtgcg agctacaggt tcatctcgtg atgaactgaa acgtatggta gatatttacc
     6661 gtgggcaaat cgtagattta acaccgaaac tttacaccat tcaattatcc ggttctaccg
     6721 agaagctcaa tgcatttatt gaggcagtta aagaagaaac aacgattgta gagattgttc
     6781 gctctggcgt gattagtcta tctcgtggcg ataaaaacgc tctgtaagcg gtcaatttta
     6841 gcttaaaatt tgcaaatccc tttatctttg agataaaggg atttttatgt agcgtgaatt
     6901 tgttgctaat tactctgttt agtttcagcg tgagtaatat aaatggtcgc agcaataata
     6961 atcgcagctc ctagccataa tttacccggt ggcacccagt taaatacaag ccaaccggct
     7021 aatacgttca aaggtaattt aataaaatca aacggctgaa tgtaggaagc atctgctaca
     7081 ctgtaggctt tggctaccgc cagttgcgct aacgccgtaa agaaaccaag taagattaaa
     7141 aagccgaaat cgctccaaga aggcagacta aacccactag ggctaaggtt cgtgagtgca
     7201 atcaacaaat taaaaggcgt aattagaata aacaggtaag cgaccatcgt agttgggcta
     7261 tcgtaacgag agagtttttt caccattagc gaataacctg cccagaaaaa agccgcggca
     7321 atcggcagta acgcaatcat ttcaaaatga tcgctccaag gctctagaat aatcatcgcc
     7381 ccaacaaacc ccattgcggt tgcaatccaa cgctttttat ccactttctc tttcaaaaac
     7441 agaccggagc cgatggtcac aaacaatgga gaagtcatca ataacgcaat tccctgccaa
     7501 ataggtaatt ggttagcgag tgccattgtc caacattgaa taccaatcgc agatagaaaa
     7561 atacggaaac agtgttgccc aaaatgctcg gtttttaatg aagagaagaa accgatatta
     7621 ttcatgctgg gaacgaggaa aataaaggca gctaaatatt gcaccaatgc aatcacgttc
     7681 gagtcaatcg gtgaaatgga cgttaatttg ggaattaagg tattgataca ggcaaataac
     7741 acaccgg
//
LOCUS       DANLUA010000118         7719 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLUA010000118 DANLUA010000000
VERSION     DANLUA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(5..763)
                     /locus_tag="PXB44_001839"
     CDS             complement(5..763)
                     /locus_tag="PXB44_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5282295.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVIHHI"
     gene            complement(783..1659)
                     /locus_tag="PXB44_001840"
                     /pseudo
     CDS             complement(783..1659)
                     /locus_tag="PXB44_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(1712..2680)
                     /locus_tag="PXB44_001841"
     CDS             complement(1712..2680)
                     /locus_tag="PXB44_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5282296.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(2774..3673)
                     /locus_tag="PXB44_001842"
     CDS             complement(2774..3673)
                     /locus_tag="PXB44_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5282297.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            3847..5310
                     /locus_tag="PXB44_001843"
     CDS             3847..5310
                     /locus_tag="PXB44_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5282298.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            5300..5605
                     /locus_tag="PXB44_001844"
     CDS             5300..5605
                     /locus_tag="PXB44_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL5282299.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            5605..6054
                     /gene="nrdR"
                     /locus_tag="PXB44_001845"
     CDS             5605..6054
                     /gene="nrdR"
                     /locus_tag="PXB44_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL5282300.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(6128..6310)
                     /locus_tag="PXB44_001846"
     CDS             complement(6128..6310)
                     /locus_tag="PXB44_001846"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5282301.1"
                     /translation="MALLADSTVDRLVAENGLVEVLGETENPKFDLTMIYAAHRYPSS
                     IVRAFVGFVLEQVKAN"
     gene            complement(6279..6989)
                     /locus_tag="PXB44_001847"
     CDS             complement(6279..6989)
                     /locus_tag="PXB44_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5282302.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
     gene            complement(6986..>7719)
                     /locus_tag="PXB44_001848"
     CDS             complement(6986..>7719)
                     /locus_tag="PXB44_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282303.1"
                     /translation="DVSNLLEVTINDEQWLVLERHDDFRELAKFGQKETYDRQMWLQI
                     RSYLVKESQFSKAKNWLAQQDFMERWMPEGNEHYGMNIHQFFAENTLNEWQEIYRHRE
                     NKSPSFKVIPSVDSHTWESTYGNDGISFYAPIPMLYHGMKMKNAIKDGYWVDLQNNLV
                     CFNPEINEKFQNSLLVKKDKFLDFLKEHKLKLVWTVLGEKHAYKESKFLQELSGVYYL
                     YGKNQQVQGSIKHFIQEIEHWGEKQ"
BASE COUNT         2442 a         1548 c         1532 g         2197 t
ORIGIN      
        1 aacgttaaat atgatgaata actaaaggtt tgccttggca ttctaacact tcggcatcaa
       61 gattaaacac tgtttttaaa ttttcagtgg tgataatctc ttttggggtg ccgatcatct
      121 taattccacc atctttcatt gcaataatag tatctgcgta agcagcagca atgttgatgt
      181 cgtgtaccac cattatagta gttaaggcga gttcatcggt taattttcgt aataatttca
      241 ttaattcccg agcatggaac atatctaaat tattcagtgg ttcatctaat aagatatgat
      301 cggtttgctg acagaaggtc attgcaatta atgcccgctg gcgttgccct ccggataatt
      361 catttaagaa tcggtgggag aaagtcgata aatcaaatcg taccagagcc tcttctacaa
      421 tggcattatc gtgcgaagtg attctgcctt gatgatgagg gtaacgccca aataacagca
      481 gatctttgac ggtaatacgg ctatgaatca cattatcttg ggtcaagata gccagttgtc
      541 gagcgatagt ttgagaagct gtgttggcta tattcagctc atttaagtgg atttcaccct
      601 gttggatagg ttgcaagcgt gcaattaaag agagcagggt ggatttgccc gcaccatttg
      661 ctccgattaa agcggtaata ccaccatttg gaatggttaa attgatatta ttcagaatcg
      721 tggcattccc gattttgtgg tgaagatttt taaccgttat cattttcttc cttaatttat
      781 ggttattttt tccgttttaa aatgagataa ataaagacaa taccgccaat aaattcaata
      841 acgacactca gaaccccttg cattttcagc acttgctcga agattgcctg ccctaaaatt
      901 aaggtgatgg cagagattaa aaaagtcatt ggtattcgca cgctatggca aacagtcggg
      961 ctaattgcat taacaatggc acaaacaagt agccctagaa atagaatcgg gccaacaagt
     1021 gcggttgaaa cagacaccaa caatccacaa cagattagta attgacgtga aaattgatga
     1081 tattctaacc caagcccaat tgccttatct ttgccaagta acaaaatatc taatttatgc
     1141 cgttgtaacc agacccaaat ggcagagaga atagcgatac agcctccaat ccaaagtaat
     1201 gtgggggtag tcaggttaaa tgaggcaaag aagagccttg agcaacggca aactctgttg
     1261 ggtcgacatt cgttgcaaca aattatttag gcttctaaat aagacaccaa agatgacacc
     1321 gattaaaagc attcgggtta aatcggatcg atttcgattc agtgaactaa atagtaatat
     1381 tgatgcccct agcattaaca aggtttcaaa gaaaaactta tagcttgggt ctaactgagt
     1441 aaagcctaaa ccgccaaata aaaatactaa cgaggtttgc aatagcaagt agagcgaatc
     1501 gaaccccaaa atacttggag ttaaaatagg attatgtgtg agtgtttgga atagcagggt
     1561 cgaaatacca atcgtataag caatagtaca gagcaaaata agctttttgc cacgcagttg
     1621 caatataaag tcccaattac ctttcgcatt ataggtcata taaaacagaa tacttgctaa
     1681 taagattaag cttaaaacga taaagcgtga tctaaccacg agccttctcc tcagcccgtt
     1741 tctctttgag caataaatag aggaaaatca ctgtcccaat aatcccaaaa atagtagaaa
     1801 tcggcacttc ataaggggca ttgatggctc gccctaaaat gtcacaaatc aggactaaat
     1861 tcgcacctaa caccacgacc gcaggcagat ttttacgcaa tcggtcacct gctatgcgag
     1921 agacaatatt agggacgacc aagccgataa acggaatctg tccaaccgtc accacgacaa
     1981 tagcggtaat catcgccacc acaattaagg caagccaagt catttgtttg tagttaatgc
     2041 ctaagttggt actaatattt tcaccaaggc ctgcaatggt taattgatct gccatcacat
     2101 aaaccacaac agccagaata gcggtaatcc agagcagttc ataccgacct gccaaaatac
     2161 cggagaagtc gccggaaaac caaatcgaaa gcacttgtag gctgtcagtt tcataagcaa
     2221 taaaagtgct gatagcttca ataacattgc caaaaacgat cccgattaat gggagcatta
     2281 attgttgatg aggcggtaat tgacgaatga gcaacataaa aagccccatt cccattaatg
     2341 cacttaatgt ggcaatagac atttttaaca gtaatgctga tgttgggaaa agtaaactag
     2401 caagtaaaat accaattgcc gcactttggc tagtgccaat catactaggc tcaataaagc
     2461 ggttcttcag cacaatttgt aataccatac ctgcaacggc taaggttgca ccgactaata
     2521 tcactgcgag cgttcttggt aaacggctga taaaaaagat ctgactttgt tcggaagaat
     2581 gaaaaatttc actccaatga aaatctgcca cacccacact ggcactgatt ggaatgagca
     2641 atattaaaag aaatagattt aatctaaaaa aagagaacat agtgataaat agcaagcggt
     2701 catattctct ctctttttta caaatgcagg cacttgcaaa aataggagga aatctgaccg
     2761 cttgtaaggt taattatttt ttctcaaatg ctgttttaat gttagttagg tcggttttca
     2821 tctgctcata accacccggc gctaaatatg aggcagcact tagataaacg atattaccgt
     2881 ttttccaagc tttagtttga cgaactaatt cattatccaa cacttgttgg gctgattgac
     2941 cttcttgtcc gatagccgca atacggtcta acacaaacaa ccaatccgga ttgagttttt
     3001 ggataaattc aaacgaaaca ggctgaccgt gaccggtatt tttaatgcta gaatctgcca
     3061 ttggaatccc taaattatcg tgaatccaac ttaaacgata acctgaacca aatgcagaaa
     3121 ttttgccacc attgactaaa ataatcagtc cgttaccttt accttttact gctgctttgg
     3181 tttggtctaa taaagtatta atttctgctt ttagtttttc ggtttccgct tctttattaa
     3241 ataatttgcc gaaagaatca agacgctgta atccgctttc gattaattta tcgccattat
     3301 ccgtcatgtc gatggttttc gcaatttggc tgacttcatc aaattttttt gctgtgcgtg
     3361 tgccaacaat gatcaaatcc ggatttaaag aatttaacgc ttctaaatcc ggctcaaata
     3421 ccgtaccgac attcaccgct ttgtctgcac tcggtttaag ataagaaagc accttaaatt
     3481 caggcatacc gtcaattttt acacctaagg cttgtaaagt atccaaactt ccggtgtcaa
     3541 acacggcaat tttagacggg gtttctttga aagtaacctc tccacgtgca gttggaatag
     3601 taacatcttt ggcaaatgca gaagcagcaa acacagcagt tgcggtcaat gcaaacaagg
     3661 cttttttaat cattggataa ctccatttat attgaaaata attctcaaag attttaacga
     3721 aaattgattt taagtaaagc ggaaaataac aagcgggcga attttgccta aaatttgcaa
     3781 atttcaattt aattaaaatt ccgatacact atgtacatca atacgtcatt atagcaagag
     3841 aaaaatatgg cagaatatag cggaacaacc caagagaaga tgaaagataa gcaggttgag
     3901 caaattaata tctcaccaca ttcgattgag gcagagcaag cggtgcttgg tggcattatg
     3961 ctcagtaatg atcactggga taatgttgct gagcgtgtac aaccgcacga tttttacaac
     4021 tatgcgcatc gaattatttt tgaacaaatg gcggagttgg tgcgccacaa taaaccgatt
     4081 gatattatta cgctggatca agccttaaaa gacaaagggg tgctgcaaga tgtaggaggt
     4141 tttgcctatt tagccgagct ttctaaaaat accccgagtg cagcaaatat tttagcctat
     4201 gcggatattg tgcgagatcg ttccgattta agaagtgtaa tcagtgccgg caatcaaatt
     4261 gcggaaatgg gctacaaaac gaaagggcgt aactccaaag aagtgttaga cgaggcagag
     4321 cgaattgtgt ttgaaattgc agaaaaacgc aataacgcca atgaaggtcc acaacgtatt
     4381 gacgatattt tgcttaaaac actcgctcga atggatacgc tatctaaaaa tcgccataac
     4441 ggcggagtta ctggtgtttc taccgggttt gttgatctca ataaaaaaac cgcagggtta
     4501 cagccttcgg atttaatcat tgtggcagcc cgtccttcaa tggggaaaac tacttttgca
     4561 atgaacttat gtgaaaatgc ctcattagtg gatattgaag ataaagatga tttagacgaa
     4621 cagggtaatc cgaagaaaaa accggcaaag ccagtgctaa ttttcagcct ggagatgccg
     4681 gcagaccaaa ttatgatgcg tatgcttgcc tcactttctc gggtcgatca aaccaagatc
     4741 cgaaccggtc agatttcaga tgatgaagac actgctaaaa tctccagcac aatggcaatt
     4801 ttacaagaac gtaataatat ctatattgat gatagctccg gtttaacacc gaccgaatta
     4861 cgttctcgag cgagacggat ttatcgtgaa aatggcggat taagcttgat tatggtggac
     4921 tatctccaat taatgcgagc gccgggattt gcagataatc gtactttaga aatcgcagaa
     4981 atttcccgtt ctttaaaggc actagcgaaa gaattagaag tgccagtagt cgctctttct
     5041 cagctaaacc ggagtttgga acaacgtgcc gataaacgtc cggtaaactc ggatttgcgt
     5101 gagtcaggtt ctattgagca agatgcagac ttaattatgt ttatttatcg tgatgaggtc
     5161 tataacgaca attccgatct gaaaggcatt gcggaaatta ttatcggaaa acaacgtaac
     5221 ggcccgattg gtcgggtgcg tttaacgttc caagggcagt attcccgttt tgataattac
     5281 acaggagggc attatgatga tgaatactaa accaacccag ccaatcacac tttgtgcgat
     5341 ttatcgcagt aaggcaaaag aaggaatgta tttatatgtg cctaagcgtg accaatttga
     5401 gcaagtgccg gaaacattac gccagttatt tggtaagcct gaatttgtga tgatgtttaa
     5461 tttaaccggt gaaaagccat tggtgcgtgc taaaaacgaa gaagtgttac aattacttat
     5521 agagcaaggc tactatttgc aaatgccacc tccgccggag aatttgcata aagcctttat
     5581 cgcacaacag aaaggggaaa aataatgcgc tgcccatttt gttccactga agaaactaaa
     5641 gtgatcgatt cacggcttgc agcaagcggt tatcaaattc gccgccgccg tgaatgtata
     5701 agctgtaaag aacgttttac tacctttgaa tcggccgaat tagttgtgcc ttatgtgatc
     5761 aaaaataatg gtagccgaga gccgtttgat gccgaaaaat taagaacagg gttaagacgt
     5821 gcattaaata aacgccctgt cagtgctgat gatgtcgaaa aagcaattag ccaaatcatt
     5881 attcagctgc aagcgatggg cgaacgagaa gtgccgagtc gccatatcgg tacattggca
     5941 atgaacgcac ttaaacaatt agacaaagta gcatatatcc gctttgcctc tatttattta
     6001 agctttagtg atattgaaga gttcattaaa gaaattgaaa aattaaaaga gtagttgcaa
     6061 aaaaacataa aaaaatgacc gcttgtgagt ttaaaatcca caagcggtca tttttcttta
     6121 aaaatactca attagctttt acttgttcca acacaaaccc tacaaacgcc cgcacaatgc
     6181 tagacggata acgatgggcg gcataaatca tcgtcaaatc aaatttgggg ttttctgtct
     6241 cacccaagac ttccaccaag ccattttctg ccaccaatct atccaccgtg ctgtcggcaa
     6301 gcagagccac tgctttttgc catatattca atggcatagc tgtcattgct ggaaaaaaga
     6361 tagggaattt ctaccgcttg tctgccagtg cctagcgtga tgacgcttgc ttcatttttt
     6421 gcccaagcgg caatggggaa atttttcaaa tctttgacgg tgctgggcgt gccaaagcgt
     6481 gccaaaaatt tagggtgtgc cacccattta ctgccgattt gccccacttt tttggcgata
     6541 aactgttcgc cgtgtagttg cccaatccga aacgccacat caatgccatc tgccgacaaa
     6601 tccaaaagac ggtcggtcag cgtgcaatgc acttgtactt gtggataagc tgcctgaaaa
     6661 tcagctatcc acgccaaaac aggctcgcag gcagggcttg ccgacaggcg taatttaccc
     6721 tttaaattgc cttgctcttc aaacaacact tctttggcag tggcaagctg ttcaatggac
     6781 aaatgcacct gttcatacag gcgttgccct gccattgtgg gtttaacgcc tgatttttgg
     6841 cggtcaaaga gttgaatgtt aagctctttt tccaaatcgg caatttgacg gctaatggtt
     6901 gcaatcggca cattgagtcg ctcggaggct ttggagagac tgccgtgctg gataacggcg
     6961 acaaagaggc ggagggtgtt gaggttcatt gtttttctcc ccaatgttca atttcttgaa
     7021 taaaatgttt aatactgcct tggacttgtt gatttttacc atatagataa taaacgcctg
     7081 aaagttcttg taaaaattta ctttctttat aagcgtgttt ttcacccaac acagtccaaa
     7141 ctaactttaa cttatgctct tttaaaaaat ccaaaaattt gtctttttta accagtaagc
     7201 tattttgaaa tttttcattt atctctggat taaagcaaac caaattattt tgtaaatcaa
     7261 cccaataacc atctttaatc gcatttttca ttttcattcc gtgataaagc ataggaattg
     7321 gcgcataaaa tgaaatgccg tcattgccat aagtagattc ccaagtgtga gaatcgacac
     7381 taggtattac cttaaatgaa ggcgatttat tttccctatg tcgataaatt tcttgccact
     7441 catttaatgt attttcggca aaaaattgat gaatattcat tccatagtgt tcattacctt
     7501 ctggcatcca tctttccata aaatcttgtt gtgccaacca attttttgct tttgaaaatt
     7561 gagattcttt aactaaataa cttctaattt gtaaccacat ttgcctatca taagtttctt
     7621 tttgcccaaa ttttgctaat tcacgaaaat catcgtgtct ttctagaaca agccactgtt
     7681 catcattaat cgttacttcc aaaagattac taacatcag
//
LOCUS       DANLUA010000119         7573 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLUA010000119 DANLUA010000000
VERSION     DANLUA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            42..614
                     /locus_tag="PXB44_001849"
     CDS             42..614
                     /locus_tag="PXB44_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL5282304.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            645..1850
                     /locus_tag="PXB44_001850"
     CDS             645..1850
                     /locus_tag="PXB44_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5282305.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            1864..5031
                     /locus_tag="PXB44_001851"
     CDS             1864..5031
                     /locus_tag="PXB44_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL5282306.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENDLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            5259..6722
                     /gene="guaB"
                     /locus_tag="PXB44_001852"
     CDS             5259..6722
                     /gene="guaB"
                     /locus_tag="PXB44_001852"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL5282307.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            6843..7514
                     /locus_tag="PXB44_001853"
     CDS             6843..7514
                     /locus_tag="PXB44_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL5282308.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
BASE COUNT         2256 a         1419 c         1727 g         2171 t
ORIGIN      
        1 agctataatg aacggctgtt cattataggt aaggagataa aatgtctagg cgaagcgagc
       61 caaaagaaga gatggtaaac cgtattttat cggcaaccga gtcgctgatt gtgcaagaag
      121 gtttgcaaaa cctttcaatg cgtagtattg caaaagaagc agggatcgct tccggtacat
      181 tatatcttta ctttaaaacc aaagatgatt tattgcactc attggctgcc caattactcg
      241 aatgttataa cggttatatg cattttgagt ttaagcctga aatggcgtta tttgagcaat
      301 ataaagcgtt ggtcaataaa aaatggcagt ttttaatcga gcgtttagat ttggtaaaac
      361 agtttcaagc ggtgataggg attgatgaat tgattcggga aaccatagct gatgaaactt
      421 ccttttggaa taaattaatt ttagaaggga aaaagcaaag gattattgct gatctatcta
      481 atgagttgct ttatggatta agcatcggca cattggtgga tatccaatat ctgcaaaatc
      541 tgaatcaaga aaatcagttt gatggatatt tagacgagat tattttacgc acttggaaag
      601 caatcacttt ttaagtttct ttttattagt tttatggaga atgtatgaca acggaaacac
      661 agaagctggg taaaggcaga aagtttttaa ttgtactcac attgttaatc gtattgattg
      721 tgtttgctgc aatcgtaggg ttacaaaaaa tcggggcaat gaaaaaagcc gaagcagcgg
      781 caaatatgcc ggaggcggtg agcgaagtta ctgctatgca agtgacgacc caagaatgga
      841 caccaaccat ttctgcggtc ggttatattc gcccaaatca aggggcaatg ttaagtgctg
      901 aaacttcagg cgtggttagc cgtgttttgg tcacttcagg acagcgtgtg aaaaaaggtg
      961 atctgttagt agaatttgac agcagtgttg aaattgctaa cttgagagcc tctgaagcgc
     1021 aattaccgaa tgccaaagca aatttagagc gttaccgtaa tctgattgcc tcaaacagtg
     1081 cttctaaagc cgagtttgat aatgctcaat ctacctacaa ccaattatta gccaatatcg
     1141 aggcattgcg tgctacgatt aaacgccgtc aaatttatgc tccgtttagt ggtttagcgg
     1201 gtatcgtgaa tgtgaatgtt gggcaataca ttacaatggg tacggaaatt gtgcgtgtgg
     1261 aagatcaatc ttcaatgaaa gtacgtttta ccttgccgca aactaattta gagcaaatct
     1321 cgattgggca aaaagtgaca gccgtaattg atgcgttacc ggcacaaacc ttcccagcta
     1381 aaattgtggc tattgatccg gctgtggatc gtttaaccgg cttaattaat gtggaagcgg
     1441 tgattgaaga agggcaggaa aaattgcttt caggtatgtt tgcccgctta aatgttgctt
     1501 taccaacaga aaccggacaa atcgtcgtac cacaaatttc tatcgcttac actatgtatg
     1561 gtgaaacggt atatgtgctg caaccattat ctgatgaaga taaagaaatg gtgaacaaaa
     1621 tggcggtaca aaacccaagt ttagatgtaa acaaaatgta ccgtgcaaaa cagatggaag
     1681 tgaaaacggc tgaccgcaaa ggaaactatt cacaattatc caaaggcgtg aaagcaggtg
     1741 atctgattgt aacaggcggt ttacaacgtt taagtaataa tgcgttggtt aaagtttctg
     1801 atacagaagc cgtaggcatt accacaccgg cgaaaaacag taaactgtag tggagtgagt
     1861 taagtgaaat ttactgacat ctttattaaa cgtcctgttt tggcagtttg tatcagccta
     1921 ttgattacga tcctaggctt gcaagcaatc gaaaaattgc aggtgcgtga atatccggag
     1981 atgacggtct cgattgtaac cgtgagtgtt aactattcag gtgcagatgc acgtttaatg
     2041 cagacactgg ttacctcaca gttagaagag gctgttgctc aagcggataa tattgactat
     2101 atgacttcat caagctctcc aagtaccact acggtaacgg caaaaatgaa gttaaatacc
     2161 gatccgaatg ccgcactttc cgatatttcg gcaaaagtga atgcggtacg ctcaaattta
     2221 ccgagcggta ttgatgatcc gtcaattaga gtctcaaccg gttcaaatga tgcgttaatg
     2281 tatatccgat ttgtctctga tgaacttaat acatcacaag ttaccgatta tattaaccgt
     2341 gtagtaaaac cacagttctt tacggtaaat ggtgtttcta gcgttgatgt gtatggctct
     2401 gtttttggct tacgcatctg gcttgatccg gataaaatgg cgagcaataa cctatcaggg
     2461 gcggctgtat tatcggcatt aagtgccaat aacttgcaaa ccgctgcggg taattctaac
     2521 ggttactata cggtttatag aaacaaagta ctttctacca cgccttctgt gacagagtta
     2581 gaagaggtta cggtttcaac caccaaagat ggaaaaacca tcaaattaaa agatattgcg
     2641 actgttgagc tggataaata ccaagatgca gcaagagcaa cggttggcgg taaagatgca
     2701 gttgtgcttg ctgtttcggt agccgcaacg gcaaactctt taaccgtagc gaaagatatt
     2761 tatccgatgt ttgagcaggt attaaaaaat ctaccaagca ccattcaagg agatattgtt
     2821 tacgataaaa ccattgcgat tgatagttct atcaaagaag taattaaaac tattggtgag
     2881 gcaaccgtca ttgtattggt agtaattatc ctgttcttag gttcattacg agcaatgatt
     2941 gtaccggtga ttaccattcc gatttcctta atcggggtat tattcttcct gcaaatgttt
     3001 ggtttctcga tcaacttgct tactttgctg gcgttggtat tagcgatcgg cttggtagtg
     3061 gacgatgcga ttgtcgtact tgaaaacgta gaacgtcacg ttaaagaagg taaaacgcca
     3121 tttgatgcag cgattatcgg tacacgtgaa attgctttac cggttatttc aatgactatt
     3181 actctgggtg cagtatattc accaatggca ttaatgaccg gggtaacagg gacactattt
     3241 aaagagttcg ctttaacgct tgccggtgcg gtatttattt cggggattgc ggcattaacc
     3301 ctttcgccga tgatgtctag ccgaatatta aaagaacata aagaagggga agagtcagca
     3361 tttgcccgcg tggtgcatag atcactagat aaaatgacca gctgctatac caatatgcta
     3421 agcggtatta tggctgttcg tggtttcgtc ttattctttg cggtgctgat cttcgccagt
     3481 ttaccgatgt tatttggctc actttccagt gaagttgcac cgacagaaga ccgtggcttt
     3541 gtggttggta tttctaatgg accttcaaac accaatttag attataccga agcagcgtta
     3601 gcccaattta atcaggaagt aactaaatta cctgaagtat cttccttaat gacagtttca
     3661 ggctttaatg gtgcaaatag tgcattatcg attattgcat taaaagattg gaatgatcgt
     3721 agccgtgaac gtgctgcgat ttctaatgat gtggctgcaa tcggtaaaaa agtggtgggt
     3781 atggacgtga atgcaattgc attccctgaa atttcaaccg gtgaaaatga tctaccattc
     3841 tcattagtga ttactaccgc tgatagctat gaaaaattgg caatcgttgc ctctgaattc
     3901 ttgaaaaaag cccaaacttc cggtcagttc ttctttgcga atttagattt gaaatttgat
     3961 acggtcacga tgaatatgaa attcgaccgt gaaaaaatgg gtgcttatgg cgtgacaatg
     4021 cagcaggtga gtggcacatt aggggcattc ttatccggtg cgattattac tcgtgtcgat
     4081 attgactctc gtgcttaccc gattgtgtcg caagcggtgc gtaatgatcg tttaaatcct
     4141 gaagatttaa tgaattacta tgtcacgact gcaacagggc aatctatccc gttaggttca
     4201 tttgtgacga tggaattaaa aacatcacca tcttctttac ctcgtatgag ccaattaaac
     4261 tcggccacca ttggtggggt agtatctggc tcaatcggcg atgcggtaaa ttgggcgaaa
     4321 gcagaattag atcgcacttt accatcaggt tatcaatatg acttccgtgg tgaatctcgc
     4381 cagttcgttc aagaaggtaa tgcgatggca atgactttcg ctctagcagt tatcattatc
     4441 tatcttgtac ttgcaattca atttgaatct tggcgtgacc cattagtaat tttggtttct
     4501 gtgccgttag ctgtgagtgg tgcattgttg gtaatgaaca ttccaaccgc aatgaaaggc
     4561 ttgtttatgg ggattgcagc aggcactggc tcgaaagata tgatggcatt cgccaacggt
     4621 atttggacgg caggttatac cttaaatatc tattcacaag taggtctaat tacccttgtc
     4681 ggcttaatta ctaaacacgg tatcttgatg tgtgaagtgg cgaaagaaga gcagttactg
     4741 cacggcaaat cacgctatga agctattttc cacgcagcca cgattcgttt acgcccaatt
     4801 atgatgacaa cggcagcaat gattgcaggc ttaattccgc ttctgtttgc ggtgggtgcc
     4861 ggtgcaatcg cccgcttcag tatgggtatg gtaatcgtag caggcttggc aatcgggaca
     4921 ttgtttactc tgtttatctt gccggttatc tacactttct tagggcaaga acacaagcca
     4981 ttacgtgaat ttgacgagga aaaatatttc ccaacgacaa aagaagcata gttttaaata
     5041 gcaaaagagc gtagcgatac gctctttttt gttggcttac aagcggtcgt tttttattta
     5101 ttgtttgcta aaaaatcaca aaatttaacc gcttgaaagt atattttcag atagcgtaaa
     5161 cgtttcccta tttacagaaa cctagttcct ctgtataatc cgctcgcaat tttccagtac
     5221 agattttcaa tttaatcatt ttttaaggaa taattgcaat gctacgaatt aaacaagaag
     5281 ccctcacttt tgatgatgtt cttctcgtcc cggcacattc tactgtgctt cctaatactg
     5341 ctgatctttc tactcaatta actaaaacca ttcgtttaaa cattccgatg ctttctgctg
     5401 caatggatac cgttacagaa actaagcttg cgatctccct tgctcaagaa ggcggcattg
     5461 gttttatcca taaaaatatg tcgattgaac gccaggcaga ccgtgtgcgt aaagtgaaaa
     5521 aatttgaaag tggtattgtt tctgagccag tgacgatttc tcctgatatg acattagcgg
     5581 aattggctga attggtgaaa aagaacggtt ttgcaggcta tccggtgatt gatgaaaacc
     5641 aaaatttagt gggaattatt accggacgtg atacccgatt tgtcacggat ttaagcaaaa
     5701 cagtgcgtga atttatgaca ccaaaagacc gtttagtgac ggtaaaagaa aacgcaagcc
     5761 gtgaagaaat tttccactta atgcacgaac accgagtgga gaaagtgctg gtagtgaata
     5821 atgaatttca gttaaaagga atgattaccc taaaagacta ccaaaaagcg gaaagcaaac
     5881 cgaatgcctg taaagatgag tttgggcgtt tgcgtgtggg ggcggcagtg ggagccggtc
     5941 cgggcaatga agaacgaatt gatgctttag taaaagcggg tgtcgatgtg ctattaatcg
     6001 actcttcgca cgggcattct gaaggtgtat tacaacgtgt gcgtgaaacc cgtgcaaaat
     6061 accctgattt accgattgtt gccggtaata ttgccactgc agaaggagcg attgcgttag
     6121 ctgatgcagg agccagtgct gtgaaagtag gaatcggccc gggttcaatt tgtaccacca
     6181 gaattgtaac aggcgttggc gtgccacaaa tcacggcaat cgcagaagcg gcagctgcgc
     6241 ttaaagaacg aggcattcct gtgattgctg atggtggaat tcgttattca ggcgatattt
     6301 caaaagctat tgccgccggt gcaagttgcg taatggtcgg ttcgatgttt gccggcacag
     6361 aagaagcccc gggtgaaatt gagctttatc aaggcagagc attcaaatcc taccgtggaa
     6421 tgggatcatt aggtgcaatg agtaaaggct cgtcagatcg ctatttccaa tctgataatg
     6481 ccgccgacaa gctcgtaccg gaagggattg aagggcgtat cgcttacaaa ggctacttga
     6541 aagaaattat ccaccaacaa atgggcggct tacgctcctg tatgggatta accggctgtg
     6601 ccactattga agaactccgc accaaagcag aatttgtccg cattagtggt gctggtatta
     6661 aagaaagcca cgtccacgat gtgacaatta ccaaagaagc accgaactac cgaatgggtt
     6721 aatagccttt ctcactctct ccccttgtgg gagagagacc gttttaaatt acgttcagta
     6781 atttaaaaca gagagaggga ataagtggtt agattttctt aaaattttac aaaagaggct
     6841 atatgctcca agctatcatt tttgatatgg acggcgtgat tgtggatacc gaatatctgg
     6901 aattttcctt acaaaaacag tttattgaag acatcaaaga acacagccgc ccgattactt
     6961 tagaacaaca atcggaagtg gtaggcaaat gtttaaaaga aattccggtg attatcaaaa
     7021 aactaagcga atccagtttg ccgattgagg aaattcgtgc acgttactac gcctttttcc
     7081 aagatctgtt tagcaaagtg gattttaaaa ccattttcag agccgatatt cagcaaatta
     7141 ttcaatttgc caagcagaat caaattaagc ttgcggttgc ctcttcttcc gccctttcgc
     7201 atattgaaaa tattctcacg gtttgcggca ttaaagacga atttgattta atcgtcagcg
     7261 gtgaacagtt tgaacacagc aagccagacc caaccattta ccgctacacc tgcgaaaaat
     7321 taggcgttga gccacaaaat gcagtggcga ttgaagactc ctactacgga atgctcgccg
     7381 ccaaaaccgc aggcttaacg gtgattggtt acgaagaaaa acgaatactg attgaccaaa
     7441 gtttagcgga ttatatgggc aaagatatga aagagatttt gaagattctt caaaaatgta
     7501 atactccaag ttaataaacg gtcgattttt atagaaattt tgtcaatgtt tctacttcaa
     7561 gtacttggaa cag
//
LOCUS       DANLUA010000120         7571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLUA010000120 DANLUA010000000
VERSION     DANLUA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            16..729
                     /locus_tag="PXB44_001854"
     CDS             16..729
                     /locus_tag="PXB44_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5282309.1"
                     /translation="MILPPFNIILLWLLALILFSLGYKYAAYFFALLGILILYIFSIP
                     YTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITGIPLERMRYAA
                     YLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTTKENAIFSREI
                     LEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKLNFMHFIPQAG
                     AMNSNMLALKEWIGYWKEK"
     gene            complement(806..3004)
                     /gene="priA"
                     /locus_tag="PXB44_001855"
     CDS             complement(806..3004)
                     /gene="priA"
                     /locus_tag="PXB44_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL5282310.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(3016..3628)
                     /locus_tag="PXB44_001856"
                     /pseudo
     CDS             complement(3016..3628)
                     /locus_tag="PXB44_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3621..4520)
                     /gene="rarD"
                     /locus_tag="PXB44_001857"
     CDS             complement(3621..4520)
                     /gene="rarD"
                     /locus_tag="PXB44_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5282311.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4747..5964)
                     /locus_tag="PXB44_001858"
     CDS             complement(4747..5964)
                     /locus_tag="PXB44_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL5282312.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6152..7456
                     /locus_tag="PXB44_001859"
     CDS             6152..7456
                     /locus_tag="PXB44_001859"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL5282313.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2292 a         1661 c         1487 g         2131 t
ORIGIN      
        1 aaactgatca ccgcaatgat cctcccgcca tttaatatta ttttgctgtg gttgttagca
       61 ttaatcctct tttctttagg ctataaatac gctgcttact tctttgcctt gttaggtatt
      121 ctgattttat atatcttcag cattccctat acttcacaga aattagggga ttcattagtc
      181 gaagcagagc catttaccat agagaaatat aaaaatgctc aagcgattgt ggtgctaggt
      241 ggcgggctac gagacagcca agagttattc ggcaaactgg caattaccgg tattccgctg
      301 gaaagaatgc gttatgccgc ttatctgcat aaagaaaccg gcttaccgat tttagcgacc
      361 ggaagcagcc caaatgggac ttctgaggca aaggttatgg cagatgaatt taaaatgttc
      421 ttcaatattg agaccaaatg gctggaaaat aaggcgaaaa ccaccaaaga aaatgcgatt
      481 ttcagccgtg agattttaga aaaagagaat attcataaaa tcattcttgt taccaaccaa
      541 tggcatatga aacgggctga aatgctgttt agccaacaag gatttgaggt gatgtctgcc
      601 agcgtcggtt ctggcgatac gcccgatcat tacaaactca actttatgca ctttattccg
      661 caagccggtg caatgaatag caatatgctg gcattaaaag agtggattgg ttattggaaa
      721 gagaaataac cgaagataaa tagcagcaag gcaggttttc acctgccttc tttttgcaaa
      781 aaatcagtca aaaacaaccg cttgtctaac taatctcttg tgggtcaatg tcgatattca
      841 gcctgatatt attcggaatg tttaaacttt ccctttcttg atcaaaggca tcgagtagct
      901 gttgtaatgc tcctcggaga gggtgttgaa tcagcaataa ccagcggtaa tgtcctgctt
      961 tcttcgccat tggggctgaa aatggtggag ggagctggaa gccttgcccg ttttgttgct
     1021 ggattttttg gctaaaataa tctgctaatt gctcaagcag ctgtaaggtt tgttgattat
     1081 ctttgccggt cgctttgaac aaaacttgcg aggaaaatgg tggcaaactc attaattgcc
     1141 tcatttttaa cgcctcatca gcaaaagctc ggtagccttg ttccagcaag gttttaagca
     1201 gtgggtgatc agggtaatgg gtttgtagca ccacttcgcc ttttttctcc gctcgccctg
     1261 ctctgcctgc cacctgtatg taaagctgtg ctaatttttc ttccgcacga aaatcagttg
     1321 aaaataacgc tgaatccaca ttaacaatcg ccaccaacgt cacattcggg aaatgatgcc
     1381 cttttgccaa catttgcgta ccaatcaaaa tttggctttt gccctctcga atagcggtca
     1441 aatggttttc caatgcacct ttgcgagcgg tagaatctct gtctatacga gtcacttggt
     1501 aatgagggaa ttgctgattt agcacctgct caagttgttc tgtgccgaca ccggtagtta
     1561 ttaaatgggt tgaaccacaa tgtccgcatt ggcggggaat taccctttgg gtggcacaat
     1621 gatggcaacg caacactttt tgcttttggt ggtaagtaaa aggcttatta cacgcctcgc
     1681 actcacaaac ccaaccgcat tcgtgacaaa gtaataccgg ggcaaagcca cggcgattta
     1741 aaaacagcat cacttgattg ccttgctcta actgggtttt catcattgaa agcaagcgct
     1801 ccgacaaacc ggcagttacc cgttgcgttt ttaaatcaat aatctgctga ttggcaagtt
     1861 gtgcattgcc ggcccgagcg gttagtgtca gttcgataaa tttcccgttt tgcacattgt
     1921 gcaaactttc aagacttggc gttgccgagc ccagcacaat cggaatattt gcatttttcg
     1981 ccctaaccac cgccaaatcc cgtgcgtggt aacgccaacc gtcttgttgc ttaaacgagc
     2041 tatcgtgttc ttcatccaag ataatcacgc ccaatttttg aaactgggta aataaagcag
     2101 agcgagtgcc gattacaatc gcactttctc catttttcgc ccgcaaccac gcatttaacc
     2161 gctcagtttc gttcatattc gagtgcaata cgtcaatttc aacgttaaag cgagctttaa
     2221 agcgttgtac cgtttgcggt gttagcccga tttcaggcac aagcagcagc acttgttcct
     2281 ctcgcttaag caccgtttca attaactgca aatacacttc ggttttgccc gagcctgtta
     2341 cgccattgag taaaaagctc gcaaaagtgg tttgtgcatt taaacggctg accactaagg
     2401 tttgttgttt atttaagatc agccgattgt tagtatttac taacggaaat tcacctagtg
     2461 agtcttgcca cgcttgtgcc ataaacggca catcaatttt ctcgatatac cctttttcga
     2521 gtaagccttt ccaagcagat gggctacaag cggtcggttt ttcaaaatat tttgcacatt
     2581 cggcaagctc ggttaaccgt tccacctgct ttttggcttt tttattttca ccgataatca
     2641 gagatttccg cccttgctcc gtcaccacaa aataattcgg ttgaactcgc tcagtggaat
     2701 cgccattgcg taattttatc ggtaaggctc cggttagcac ttcaccaatc ggggcgtggt
     2761 aataacgagc cgcccagttc agcagttgcc acatttctga gtcaaaaatc gggctagaat
     2821 ccaacgctgc tttaatcggt ttgagttttt cggttggcac atcactagtt tgcgggaaat
     2881 cgaccacaat gccgattttg gtctggcgac caaaaggcac aaccacccgc ccgccttggc
     2941 aggcattgaa tgccggcggt agcaaataat caaaataacg atgtaacggc acattcagtg
     3001 ccactctgat taatctcact tctctaaacc aattttgatt gcaagcccga taaatatcga
     3061 gccgcagatt ttatttaacc aatttccaaa ggtttgatta tgccgtaaaa aacgcccaac
     3121 aggtgcagcg gctaagacta aaatacttac ccaaataatg ctggtggata aaaacgttag
     3181 ccccaacagc ataaaaggta ttttccaaat tactggatac aaactgcggc aataacgcca
     3241 agaaaaagag caatactttc gggtttaata gattggtaat caacccttga cggaatatct
     3301 tccatagagg aagtttgtct agattgattt tctctaacac caacggctct cgccacattt
     3361 taatgccgag atagcagaga taaattgccc ctgcgtattt cagaaaatta aataaagtgg
     3421 gcgaatgtgc cacaatgcct gcaagcccga gtgaaaccgc aagaatatgg cataaaatgc
     3481 ctgctagaat gccgaatgaa gacattaagc ccgacttttt cccctcagca atagtgcgag
     3541 ttaaaatata gatagtatcc gaacccggtg taagatttag cagaatacaa gcggtcaaaa
     3601 atccccaata atttacaata tcactcatta ttcacactca atttacgttt acgctgctga
     3661 cgaagagcaa acataccatc taatactaaa aatgaaatgg agatcaacaa gcaaatcatc
     3721 aacaagtaag attcatttgc taacctttcg ccaattaaga aagacaccac caacatcaat
     3781 agtggctcaa catagcctaa taaaccaagc aaattaaagg gtaaaatcgt gctggctagc
     3841 gtatagctga taagtgcagt gccactaatt aaacccagta atgcaataaa atagtaaata
     3901 ttgaggtttt gtgtttcgac atattgcata tcggtttgag agataaaata acaagctaca
     3961 gggagcaaat ataaaatttc taccacaaag ctatgtaaat ggcttaagcc aaactttcgg
     4021 cgaaggtaaa aatagattgg gtaacctgtg caaacaaata aacttgctat cgaaattttt
     4081 cccgtaaaca aaatattact cgctacacca atgaaagcaa ccacaatcgc caaccattta
     4141 ttcaaggaaa gcgtttcttt ataaaccact ttgccaaatg ccaccataat aatcggcatt
     4201 aatagatagc ccattgaaac ctcaatcgct tttccgctat tgggagccca taaaaacagc
     4261 cacatctgcc cgcccacaat agatgccgtg attaaaataa ccagcaataa ataaggctct
     4321 tgctttaggc gttgaagaaa tacccaaaac tcattttgtt ttttaaataa gtataaagca
     4381 aggaaaagaa aaggaatcgt aaccagcata cgaatgccga aaatgctcac acctgccagc
     4441 ggttttagaa agatcgccaa atagtacata cagccgaata acactgaggc tgaaagagat
     4501 aaaagaatgc cttttaacat agtgataaga aagtaattat tttttaattt ttcaaatctc
     4561 ccctacccct cttttctaaa gaggattgtc gagttctaaa taaatgaata atgttcggag
     4621 gtaggggcgg gtccagtgcc cgcccgcaaa ttaatatcgt tagcggacag gcacaggacc
     4681 tgtccctact gatattatag aacttacaac cctgctttta taaagtgggg taaagggaga
     4741 ttcgacttag tcttgacgat aggttttcaa gaaattcgca aggcgaccaa tcgcttcttc
     4801 aatttgatgt gcataaggca aagttaccac acggaagtga tccggttttt tccagttaaa
     4861 accggaacct tgcactaata gcactttttc ttgtcgcaac aaatcgtaga tgaatttttg
     4921 gtcatcttta atgccgtaca tttcggtatc aattttcggg aacatatata acgccccttt
     4981 cgcttttaca cagctaatac ccggaatttg taccaataat tcgtgcattt tatttcgttg
     5041 ttccagcaaa cgaccgcccg gcaacacaaa ttcattgata ctttgataac cacctaatgc
     5101 ggtttgaatg gcgtgttgca tcggcgtatt ggcacataaa cgcatagaag agagcatatc
     5161 caacccttca ataaaacctt tggcttgatg cttcggaccg ctcagcacca tccagccctg
     5221 acggaaaccg gcaacacgat acgccttgga taaaccgtta taagttacgg ttaatacgtc
     5281 cggtgcaaga gcagcaatat ggtgatgtac cgcaccatca tagacaattt tttcataaat
     5341 ttcgtcagca aaaataatta agttatgctg acgggcaagt tctgcaattt ccagcaaaat
     5401 ttgacggcta tacactgctc cggtcgggtt attcgggttg ataaccaaaa tccctttagt
     5461 gcgaggcgtg attttcgatt taatatcttc aagatcaggg aaccactcat tttcttcatc
     5521 gcacaggtaa tgcactgctt taccaccggc tagggttgaa gctgctgtcc ataacggata
     5581 atcaggcatt ggaattagaa tttcatcgcc atcatttaat aacgcctgca tcgacattgt
     5641 aatcagttca gatacgccgt tgccgatgta aacatcgttt acatccatac cacgcatacc
     5701 ttttgattga taatattgca caatcgcttt gcgggcagaa tacaaacctt ttgaatcaca
     5761 atagccttgt gcggttggaa gattacgaat cacatccact aaaatttcat cgggtgcttc
     5821 aaagccaaaa ggggctgggt taccgatatt taattttaaa attttatgcc cttcttcttc
     5881 taagcgaagt gcctctttat gaattggccc acggatatcg taacgaacct gagctaactt
     5941 atctgatttg ggaaaacgat ccattgtaaa cttcctataa tgtattatct aattgtagtc
     6001 tcatcccgct gttcaaaaaa tcggtaattc ggaatgaatt tgtaaacatt actctaattt
     6061 gggaggaaaa aaaagaggaa atacgttaaa atggagacct ttttttcaat cagctaaaaa
     6121 caagcggttt ctttttctga tttttttgca aatgcttatt tttcatcaat taaaacaaga
     6181 acttacaaaa agttactcaa aagtgggaag agagcaagga ttggtcgcca ttcaagcctc
     6241 tatgccgcta ttggaggcaa atattccgct actcggttgg ttgaaaacac aaaaaacaag
     6301 ctaccctcac ttttttctgc aatatcgtga tacagatcag accattgcca ccattggcac
     6361 acttcgccaa ttttatacgc tagaagctgc acaatctttt attcaacaac atcatttccc
     6421 gttaatcggc gggcttcaat ttgaaggcaa tacgcaattt attttgcctc aattttcatt
     6481 ggtaaaaaat cagcaaaatt taaccgcttg cttctatttt gatagtgata actttgaaca
     6541 acaagcggtt atttttgaac aatttcttgc aaatttcagc caaattaccg aattaggact
     6601 aacggataat ccgctcgttt caatgagtgc caccagccat tttgagcaat ggaaagccaa
     6661 tattgaaaaa gcgattgttt ctattgagca gggcgatttt caaaaagtgg tattggcaaa
     6721 tgcgatgaga cttcattttg agcaacccat ttgtgcttac agcttgctgg cacaaagcca
     6781 aaaaacaaat ttaggttgtt accacttttt atgggcggaa agtgctgatg ttgcctttct
     6841 tggctcaacc cctgagcggc tatatcaacg ccaagggcta cacttttcta ccgaagccct
     6901 cgccggcacc gctgcggtta ccaccagtga agtgcaaacc gaactcaatg cacaatggct
     6961 gttaagtgat cccaaaaata tctatgaaaa tcaattagtg gttgatgaca ttgaaaacca
     7021 actcaaagat tgcgtgaccg aatttgcagt atccgaggca aaaattaagc gtttgcataa
     7081 tgtgcaacac ctacgccgcc aaattcaagc aacgctcaaa ccggacacct cagattcaga
     7141 ttgcttaagc aaaatccacc caactgcggc agttgcaggg cttccccgcc aaaaagcaaa
     7201 gggttttatt accgaaaatg agccttttac ccgccattgg tatgcaggta cattaggcgt
     7261 aatgggcaac gaggaagccg aattttgcgt aacactccgt tctgcattaa tttcacaaaa
     7321 ttcaattacg ctttatgccg gtgccggcat tgtgtcaggc tcagatccac aatcggaatg
     7381 gcaagaaatt aaacgtaagg cacaaggaat ggcaaaactg ttggatgttg atttagtaac
     7441 aaaaggggaa ctgtaagcta ggctttcaga acttaatctc ttaattattt ctttctatat
     7501 tttcatttcc aacttcccaa caaaatatat tattgccacc aaattagtag tgaatattcc
     7561 ataattaaaa a
//
LOCUS       DANLUA010000121         7568 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLUA010000121 DANLUA010000000
VERSION     DANLUA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            172..840
                     /locus_tag="PXB44_001860"
     CDS             172..840
                     /locus_tag="PXB44_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282314.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            837..1718
                     /locus_tag="PXB44_001861"
     CDS             837..1718
                     /locus_tag="PXB44_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282315.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1718..2533
                     /locus_tag="PXB44_001862"
     CDS             1718..2533
                     /locus_tag="PXB44_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5282316.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2530..3666
                     /locus_tag="PXB44_001863"
     CDS             2530..3666
                     /locus_tag="PXB44_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5282317.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3694..4092
                     /locus_tag="PXB44_001864"
     CDS             3694..4092
                     /locus_tag="PXB44_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5282318.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4127..5830
                     /locus_tag="PXB44_001865"
     CDS             4127..5830
                     /locus_tag="PXB44_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5282319.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            5942..6586
                     /gene="adk"
                     /locus_tag="PXB44_001866"
     CDS             5942..6586
                     /gene="adk"
                     /locus_tag="PXB44_001866"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL5282320.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6648..7463)
                     /locus_tag="PXB44_001867"
     CDS             complement(6648..7463)
                     /locus_tag="PXB44_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5282321.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRAEAESIKPQVEERVAENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQ
                     ASVVNEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2220 a         1489 c         1489 g         2370 t
ORIGIN      
        1 agattttcat ctactcattt tcattttgat tgatttggtg gggtaatccc acctttttta
       61 tgctcttaaa acacaacact atgattcaaa taaattccat tagctactct gttgaacaaa
      121 accgtaaact gctaagcaat atttccctca atttaagcaa ggggcgttta tatggcgcat
      181 aacggctcgg gtaaatcaac gttaatgcgt ttactaagcg gagatataac gcctaccagc
      241 ggttcaattt tagtcgatgg attaccactt catcagcttt ctaccaaaga agcggcaaaa
      301 catattgcct atttaccaca acgtttacct gaagcggcgg atttcttagt gcaggaactg
      361 gttgctttag ggcgatttcc atatcaaaaa tggctacaaa aaccaacaga atttgatgtt
      421 caaattgttg agcaagcaat ggaaagcacc tccgttactc agtttgctaa acagcctgta
      481 aatagtctat caggcggaga aaaacaacga gtttggcttg ctatgtgctt agcccaacaa
      541 acaccctatt tactgctaga tgagccattg gctgcccttg atattgttta tcaagttgaa
      601 gtaatgcagc ttattcgcaa gctagtgaat gaacaaggtc taactgtcat tatcattatt
      661 cacgatatta atcttgcctc ccaattttgt gatgaaatta ttgcgttaaa gcaaggcgaa
      721 ctctgtattc aaggttctgt tgatgaaatt atgcaagaac agaaattact tgagatcttc
      781 gggctaaaac tgcacttgtt taaccaccct gcaggcttac ataaagtggc ggtattatga
      841 tgtttaggca atacaagcgg ttcatttttc tctttatttt gcaaatttct accgcatttg
      901 ctcaacctaa tatttttaca ccagattggt ctattgcctc tgcccttact gaaatgggaa
      961 atccgcccat tgcgatgggg gataagcgaa tttatccgct atggtcgcac cgccctattc
     1021 agcctaatag cgtgttagat attggagcgc gttatcagcc taatcgcgaa ttattagcac
     1081 agcttcctgt tgaattggtg ctatcaaact ttttctacgc tcacttggct tctgtttacc
     1141 cgaaagaaat tccacaaata gacgtgctat ttgatggtgg caatactgaa cctcagcaac
     1201 attggcatac ttaccaaaaa gccactcgta tgattggaac agagattaaa aaccctaatg
     1261 cggcagaagc atatttaacg aaaactgaac aaacgttggc tcaatggggg aaagaaatcc
     1321 ttaacagtaa tccagatatt gattactact ctgttgttca atttggctct ccgagagagt
     1381 tacgcatata tgcgtctaat tcaatgttcc gtgtagcctt tgatttgatg ggcttaaaac
     1441 aagctgattt aggtgtggga gatcgctggg gaaataaact tattcctgtg agtgatttag
     1501 ctaaattacc cgaacgcacc tgtttaattg tcatctcacc tttttctaaa atgacgcaag
     1561 cagatttaga aaatagctac atttggcaac gtttaggatt tggtaaaaac cgttgtatga
     1621 aagtattacc gcctgtttgg ctctttggtg gggctgattc catctcgcac tttgctgagt
     1681 ttttacacaa tgcaatgaca caaggagaaa aagagtaatg aaatcatcat tcttattgat
     1741 tggctgtatt cttttatcct tagtaacagc tgttatccgt ttaaatatgg tctgggagtg
     1801 ggaatggcag ttactactca taaatgttga tttattgcca attaatcaag ctgcggtaaa
     1861 agcagctctt ttacctgaaa tgacaacgtc atttttagca ggcggtttat tggctatagc
     1921 cagcagtggc ttacaacata ttgtcagaaa taatttagcg tcagatagca cacttgccgt
     1981 cagtagcggg gctcaacttg cattgatgat tgcggttatc tttttcccag ccgccgagct
     2041 tttttctagt ttctggattg cgttacttgg ctctttagct gctatcgtac tggttttatt
     2101 tattgctaaa gcctctaagc tcaatcccct tacccttatt ttagggggat tgattgttaa
     2161 tattttgttt ggagctattg cctctttact gacccttttc tactttgatt ttttatttgg
     2221 cgtgatggtt tggggaagtg gctcactttt acaagatggt tgggcaactt ctattacctt
     2281 agcgataacg gtagtagttg cattttttat ctttgtctta ctctctcgtc cactcacaat
     2341 tttaagttta gatgatgaac aagctcgccg cttaggcgcg cctgttaatt tactacgcta
     2401 cttagttatt tttgtttgtg ccgccattac agcattggtt gtaagcaaaa taggtgtgat
     2461 tggctttatt ggatttgcag tgcaagtttg gctcattttg ccaaaatacg acatttatta
     2521 cttcgcctct tgattgcttt tctagcgggc ggatttttac tcttttttac caataatctc
     2581 atcggcattg ttagcagaat ttacgacact ctactacctg ttggtgcatt taccgcaaca
     2641 ctaggcgcac cattattaat tttcttggta ttacgccaac gtaaagcaca atttgaagca
     2701 agcaatacag catacttccc cgttaaacgc ctcaattcgt ggaaactgcc aatattgttg
     2761 ttatgcgctg gagttatcct attttgtcta ttacaaatca ttgctccaac aatgaatggc
     2821 tgggctatta gcattgatga aaacctgatg agttcctacc gcttgcctcg tagtctaagt
     2881 gcgatagcaa caggggcaat gcttgcggta agtggtagcc tattacaaat cttaacgaga
     2941 aacccaatgg caagccctga agtattaggt gtgagttcag gggcaactat tgctgcagta
     3001 ctgtcattct tacttttccc aacattatct agcattagct tcttgttatt tggtgtatta
     3061 gggagtttga ctgttctcgc tcttattatg tggctctctc gtaaattaca accatcagcg
     3121 ttaatgatta caggtgttgc tattggagct ttagtcagtg gcttaatggc tattattcaa
     3181 ctaagcggca gccctcgttt aacggcagtt attagttggc tgtctggctc aacctattac
     3241 gctaatccga atacagtttg gattttatta tctgttgcaa tcctacttat cagtgccact
     3301 ttactgttta tcaaaccttt acgtttgatc tcacttggcg agaccgtttc ttaccattta
     3361 gggttagagg ttaaaaaagt acaaatgatt gtattacttt taattgccct aatgagtgcc
     3421 gcctctacac ttgcggtagg gccaatgagc ttcattgggc taatgacccc tcatttagct
     3481 aaaaatttag gtgcaattac accggagaaa cagatcccta tatcggcatt attaggggca
     3541 atgctaatgc tcattgccga ttggctcggg cgttatctaa ttttccctta tgagattggg
     3601 gcaggcatta ttgcctctat tctcgggggt atctatttct tactgttact caaacaacat
     3661 aaataatttt ttattcatta cttaggagaa attatgaaca cgctatctaa agcgataaaa
     3721 cttgccttat acagttctgg tatagggttt atctcaacag cttacgctga aacagttact
     3781 catcttgata ccgtagaagt aagtgccgaa acgccaaatg cctcatcgga agaaactgga
     3841 cgttatgcac agaaaaaagc caaaggctct aatggaatgg cattgagtgc gaaagacacg
     3901 ccacaaagcg taacggctgt aacgcatcaa caaatcagag atcaaaatct taatacgatt
     3961 gccaaagcac ttgaagcaac tcacggtgtt tctgtttctt tacttgacag aggacgctat
     4021 tctttcagtg ctcgtggttt tgggattgat aaagtcaagg tagatggtat ggatctcaaa
     4081 gtaagtaaat aaccaatgga caacgggcga aaatacaggc aacagcgtgt tatacgaccg
     4141 agtagaagtt gtgcgtggtg ctacaggctt aactactggg gcaggcgatc cttctgcaag
     4201 tgttaatctt gttcgtaaac gagccaatag catttcccgt tatactattt tagaagctgg
     4261 aatcgggcgt tatgctgatt tcaatacaat gattgaccat agccaagcat tgaataattc
     4321 aggatcagta agaggccgct ttattgcaga gcataaaaac ggtaatacgt tcattaaaaa
     4381 tgaaaaagaa cgttacacca cgctttatgg caatattgaa gcagacttaa ctgacactac
     4441 caaacttggg ttaggtgtaa gctatcaaaa agaaaaacgt gatgcagcac tttggggtgg
     4501 tttacccgca ttctatagcg acggtggtga aactaactgg agcagaagca aaaatgccag
     4561 tccggaatgg gcttactggg ataatgaatc cgtaaactat tttgccgata ttacacaaaa
     4621 actaggcgaa aaatgggaac ttgcattaaa aggcaattac cgtgtaggca aacataattc
     4681 agaacttttc tatttcagcg gctcatcact taatcgccaa gatggattag gttggtcgcc
     4741 ttggccgggt aaatttaaaa atgataatac acaatctaat gtgcagcttg atcttaccgg
     4801 ttcattctct gcttggggat tagaacacga tgcggcaatc ggtattcaat acaatcgagc
     4861 acatcgtaat tcctacgctg ccgataatag cacctttgac ggagctttcg actttaataa
     4921 ttggaatggc atttatgcta gaccaaactg gggaagtatg tctgaaaaat actaccaaaa
     4981 cttaaaagag ttcggggtgt atgtttctac ccgtttacat cttacagata gactttctat
     5041 gcttctaggt tctcgtattt catcgtaccg cacagaaggc actttctatg gttcaacaca
     5101 aaacttccac gctaacgatg tttggacacc ttatgcagga ttaacctttg atattaatcc
     5161 acaaaatacc gtttatgtaa gttataccga tattttcaaa ccacaaagtc aagtggacat
     5221 caataacaaa ctactctctc ctgttacggg cgaaacctac gaagcaggtt ggaaagggag
     5281 ttatttcaat gacaacttgc aaactcaaat tagcgtattt gaaacaagac aagataatct
     5341 agctcaatct gccggtatta accctaatac attattagcc tactacacag cctctaaagg
     5401 tgcgaaagtt cgaggctttg aagtagaagc atctggaaat gtaactgaaa aattaaaact
     5461 cagtgcagga tatactcagt ggaaaggcga tgatgcttca ggtaacccaa ttaacacaac
     5521 tcaaccacgc aaacaattta aactctttgc aacttacgat atgaaccgtt tagtgcaagg
     5581 tttaacggta ggagcaggtg taaactggca aagcagaatt tataccaaac acgctactta
     5641 tggcgaatat ggtcaaaaag cttacacttt agtgaatttg atggctcgtt atcaatttaa
     5701 tgacaacttc tcagcacagc ttaacattga taacttattc gataaaaaat atcgtaacgc
     5761 cctcagcttt agccaatata gctacggtga gccattctat acacgcttaa atttacgcta
     5821 tgaattctaa ttccgtataa caagcggtca aaaaattaaa aaaatttgca attttaggca
     5881 gtaatcggta aggttactgc ctaagtgatt ttattatttt tagtttaatt gaggttttca
     5941 aatgaaagtg attttattag gtgcgccggg tgcgggcaaa ggtacgcaag ctcagtttat
     6001 tatgaacaaa ttcggtattc cgcaaatttc aaccggcgat atgttccgtg cagcgattaa
     6061 agaaagcact gagcttggta aacaagcaaa agcattgatg gacgaaggca agttagtgcc
     6121 ggatgaatta acggtcgcct tagtaaaaga tcgtattgct caaccggatt gtgctaatgg
     6181 cttcttatta gacggtttcc cacgcaccat tccgcaagct gatgcgttaa aagagtcagg
     6241 tgtaaaaatt gatttagtgt tagagtttga tgtagctgat gaagtcattg ttgagcgtat
     6301 gagtggtcgt cgtgttcacc aaccttcagg gcgtacttac cacgtggtgt acaatccacc
     6361 gaaagtggaa ggcaaagatg atgtgactgg tgaggattta attatccgcc aagatgacaa
     6421 acctgaaacg gtattagagc gtttagcgat ttaccacaaa caaaccaaac cgttaatcgc
     6481 ctactacacc gcagaagcag aagccggcaa cactcgttat gagcgtttag atggaacaaa
     6541 accagtggaa gaagtaagtg ctgagttagc gaaaatttta tcttaaatgg attattaaaa
     6601 tgaacaagcg gtaatatttt tcgagaatat taccgcttgt tctttgctta aacacaatat
     6661 ttggtgagca ttaagatatc atcataaatc ggtgaaataa acacttccgg attttcaatc
     6721 ttcatctcct ttggtttttc ttctagcttt tctggctctt tcttatcttt attctctacc
     6781 ggttgagtcg cttcatttac cacactagct tgagttttat ctgctacagg tatatctatt
     6841 ttgtttggtg cttgctctac tgcttgagaa tccgctttca gctctttaga aacttcagta
     6901 tttacgatag gttcttgtaa gttttccgct actctttcct ctacttgtgg cttaatactt
     6961 tctgcctctg ctctcggttt ataagatgga tcaagtaatt gatggctaat atccattttc
     7021 ggatcaagcc aaattgctac aatagcatca ttgaaagctt tattaaacac atcagagact
     7081 actttatcat ttaaaataaa ataacctcta tcttctaatg caatatagtg caatgtatct
     7141 tcaggcttaa tatctgggaa agtttttctc aggtgggtaa taacctcatc taactctttt
     7201 tcaggtagct taatgcctaa attattccaa gttcttgcta aagaaaccgc aaaatcacgc
     7261 ccctcccacg gttttttata ttgtagagta agcattaaag gacgtgtacc ttctacataa
     7321 gtaccgctct cagtaaaaag cgtaatatgg taaatattaa atggccccca cgtataattc
     7381 acatcattaa tttttgtcca atttgcattt gctgttgata aaaatagaga aaacaacagc
     7441 gaaaagacca ctttatagct cattattttt gtgtccttaa atctaaaatt cctactcatt
     7501 attatagcct taaatatggc tcacacacat tataattttc gatataaatt ttgtgatcag
     7561 gttcaaaa
//
LOCUS       DANLUA010000122         7541 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLUA010000122 DANLUA010000000
VERSION     DANLUA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(19..1302)
                     /locus_tag="PXB44_001868"
     CDS             complement(19..1302)
                     /locus_tag="PXB44_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5282322.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            1468..5184
                     /gene="metH"
                     /locus_tag="PXB44_001869"
     CDS             1468..5184
                     /gene="metH"
                     /locus_tag="PXB44_001869"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5282323.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
     gene            5292..7295
                     /locus_tag="PXB44_001870"
     CDS             5292..7295
                     /locus_tag="PXB44_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL5282324.1"
                     /translation="MIEKQNYHLVQLLKDSNYQLTQFSPAYIKKLEESIFLKSGKNGD
                     IPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYDYPFERMELEYSVVFGREKKRADIV
                     IFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGVWTNGESISYYNRRDPNY
                     FKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLKDLVIEMEDEVLANAGVD
                     VFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKAKIQSLFDRAKNKWEGVF
                     AESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYV
                     IDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKARGLERSHLFTAEEKLPE
                     QTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNTLDYERWEDFTKEEEWND
                     VYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETRILARYELGKNSKGKQQS
                     KVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRDFIAERCRILAVVGLHGN
                     VFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSKDNSGDKIYVKNADGTLK
                     LDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVGKD"
BASE COUNT         2333 a         1577 c         1735 g         1896 t
ORIGIN      
        1 tcttattttt atgaatagct attcagcttg tccccaagga gcaaccttaa acaacattag
       61 cattcccgga ataccaataa agaaacagag ccagaagaat tggtaatagc ccatcatttc
      121 cactaaatgc cctgtgtatg cccctaacat tttgcttggt agggcagcaa ggctggtgaa
      181 aatggcgagc tgcattgcag tgtaagcggg attagtttca cgagccataa aggcaacgaa
      241 agccgctgtg cctaaaccca cgcccagata ttcacctgcc atcactaagc ctaatttcca
      301 aagctcggca gctccaatgc tttcggtagg gaaatgctca aaactggcaa ggtaagcgaa
      361 gcctaaaata gtaattaatt gtacaaatcc gaacagccat aaggctcggt taacgcccaa
      421 tttcaacatt gccacgccac caataatacc gccgccaatg ctgcaccaaa gtgaagttat
      481 tttcacaatg ccggcaatgt gcgatttagt aaaacccata tccaaaataa atttggtttg
      541 tagcgtagtt gcaagggaat cgcctaattt atataggaaa ataaatagaa tcagcccaat
      601 cgctcctgcc acgcctttac gggtgaaaaa ctctttgaac ggatcgacaa acgccatata
      661 aaacggctta gaacggtcga ttggcttatg attcggctcg gcagcgatta ttagcgtaac
      721 gaataagcaa ggcactaaaa aagcagcggt aatcagaaat acggtatccc acgcatagtg
      781 atcggctaaa aataatgaaa gcccacccgg aatcagccct gcaatacgat aagcattaac
      841 gtggatagag ttacctaacc cgagttcgtt atccgataaa atttcacgcc gaaaggcatc
      901 caccacaatg tcttgcgtgg cggaaagaaa agctaatagc acagaaagag taacaataat
      961 tgaaatatct tgcactgggt tgaacaagcc aaagctggct aaaatcacca atagcccgat
     1021 ttgtgaaatg aaaatccagc ttttgcgtct gcctaaaaaa ggtggaaagt agcgatctaa
     1081 caatgcagcc cacaggaatt tccacgtgta gggcaaagag gtcagggtga aggcggcaat
     1141 agttttaata tctaaattgg cactggttag ccaagccggc attagctgaa ttaacacaaa
     1201 caacggtaaa ccggaacaaa agccggtgta aacacaaagc aacatatttt tagtaaaaat
     1261 ctgttgccag atagagcgtt tgggttcgtg acaagcggtc atatttttcc tacattttgc
     1321 aaaataaaaa ggggaagaac tgagccaata tagcataatt ttaaaggcga ctctaaccct
     1381 ttccatttcg tcacagataa ggtttgacag ccttgcgaca tatttctatt attcctttta
     1441 atcaaactaa aaaactggaa gcagagtatg aacacgcagc atagaaccga attacttcaa
     1501 aatgccctta ccgaacgcat tcttatctta gacggtgcga tgggaacgat gatccaaaaa
     1561 tataaactaa ccgaatctga tttccgaggt gaacgtttta aaaacagtac catcgatctt
     1621 aaaggcaata acgacctact cacactcacc cagccactcc tgatttctgc tattcacgag
     1681 aaatatttag ccgccggtgc ggatattatc gaaactaata cctttagtgc gacgaccatt
     1741 gctcaagcag attatgaact tgaaagtatt gcttatgagc tgaatcttgt gggggcaaaa
     1801 cttgcccgtc ttgccgccga taaattctcc atccctgaaa aacctcgttt tgttgccggt
     1861 attcttgggc cgactaaccg tacttgttcg atttcccctg atgttaatga ccccggtttc
     1921 cgtaacatta cctttatgca actggtggaa gcctatgccg aggcaacccg tggtttgatt
     1981 gaaggtgggg cggatctgat tatgatcgaa accattttcg ataccctgaa tgccaaagcg
     2041 gcggtgtttg caattgaaac ggtgtttgat gaactgggcg tgaaattgcc gataatgatc
     2101 tccggcacca ttaccgatgc ctcagggcga acgctgtcag ggcagaccac ggaggcgttt
     2161 tataactcgc tccgccacgc caaaccgctg acgttcggct tgaactgtgc gctcgggccg
     2221 aaggaactcc gcccttatgt agagatgatg tcgaaaatca gtgagacttt tgtttcggtt
     2281 cacccgaatg ccggcttgcc aaacgctttt ggcgggtacg atttaggggc ggaagagatg
     2341 gcgacttaca tcaaagagtg ggcagaactc ggctggctga atattgtcgg cggctgttgt
     2401 ggcaccacgc cggagcatat ccaagccttt gccgaagcaa ccaaaggcgt tgcgcctcgt
     2461 aaattacctg aaattccaac cgctatgcgt ttgtcgggct tagaaccgct gacgattgac
     2521 gataattcgc tgtttgtgaa tgtgggcgaa cgcaataacg tcaccggttc ggcaaaattt
     2581 aagaagctga ttaaagaaga aaaatttgcg gaagcgattg aaattgccat cagccaagtg
     2641 gaaaacgggg cgcaggtgat tgacgtgaat atggacgagg cgttgctcga ttccaaaaaa
     2701 tgtatgaccc gtttcctcaa cattcttgct accgaacctg aggcggcaaa agtgccgatt
     2761 atgattgact cctcgaaatg ggaggtgatc gaggcgggct tgcaaacggt gcagggcaag
     2821 ccgatagtga actccatttc actcaaagag ggcgaagaga aatttattca ccaagccaaa
     2881 ctctgccgcc gctatggggc tgcggtggtg gtgatggcat ttgacgaagt cgggcaagcg
     2941 gataccgaag aacgcaaggt agaaatttgt acccgagctt atcggatttt ggttgatcag
     3001 ctcggcttcc caccggaaga cattattttc gacccgaaca tttttgcgat tgccaccggt
     3061 attgaagaac acaataacta cggcgtggat tttatccaag cctgtgagcg gattaaacga
     3121 gacttgccac acgccaaaat ttccggcggg gtgtcgaatg tctctttctc tttccgaggc
     3181 aacaatgtgg tgcgtgaggc tattcacgcc gtgttcctct actatgcgat caaagccgga
     3241 atggatatgg ggatcgtgaa cgcagggcag ttggcgattt atgacgacct cgaccctgaa
     3301 ctgcgagagg cgattgaaga tgcggtgctg aaccaccgtc atgacactac cgaccgcctg
     3361 ttggaaattt ccgaaaaata tcggggcgta aaagtagaaa gtgccgatga aagtgccgcc
     3421 gagtggcgaa atttacctgt ggcagagcgt ttaaaacacg ccctcgtaaa aggcataacg
     3481 acctacatta ttgaagatac cgaagaggct cgccagcaat ttgcctctcc gctggaagtg
     3541 attgaagggc cgctcatggc gggtatggac gtggtgggcg acctgttcgg agacggcaaa
     3601 atgttcctgc cgcaagtggt gaaatcggct cgggtaatga aacagtccgt tgcctattta
     3661 gagccgtaca tcaacgccac caagcagaaa ggatcgagca acggcaaaat ggtgatcgcc
     3721 accgtgaaag gtgatgtaca cgacatcggc aaaaacatcg ttagcgtggt gttgcagtgc
     3781 aataactttg aagtgataga cttaggcgtg atggtgcctg ccgatgtaat tatcgaaact
     3841 gctattcggg agaaagccga tctcattggg ttaagcggac taattacccc atcgctggac
     3901 gaaatggagt attttttagg tgaaatgaac cgcttgaagc tcgatattcc ggtgctgatt
     3961 ggcggcgcaa cgacctcaaa agagcatacg gcgatcaaac tttatccgaa atataactac
     4021 cccgtagtct ataccgccaa cgcttcacgt tgcgtaaccg tctgtgcgac cttgatgaac
     4081 cctgaggcaa aagccgaatt ttgggaaaaa accaaagccg aatacgaaga aattcagcgt
     4141 aaatttgccg ttcgcaaacc acttcgcaac ggtttatcta ttgaggaggc tcgagccaat
     4201 cgttttgacg gatttagtgg cgaatgggca gactatgtgc cgccaacgcc aaaacagacc
     4261 ggcattatcg agtacaaaaa tgtaccgatt gccaccctac gcaaatttat cgactggtcg
     4321 ccgttcttta tgctctgggg cttaatgggc ggttatcctg atgcctttga ttatccggaa
     4381 ggcggcgaag aggcacgccg tgtctataac gatgcccaac gggtgctgga cgaatttgag
     4441 caaaacggca agctcaaccc aagtgggatt atgggcattt tccctgccgt gcgtgagggc
     4501 gatgatattg caattttaaa cgaagatcgc accgcttgta tcggcaaatc ctaccacctc
     4561 cgccaacaat ccgaacgagg caaaaacagc aaaagcccgt acaacctctg tttaagcgat
     4621 tttatcgccg accgagaaag cggacaacaa gactggttcg gaatgttcgc cgtttgtgcc
     4681 ggcattgaag aacactattt agttgaaagc tacaaagcgg caggcgatga ctacaacgcc
     4741 attttgctcc aagccgttgg cgaccgcttg gcagaagcaa tggcggaata cctacacttt
     4801 gagctacgta ccaaagtctg gggctacacc gtggaagacg aagacaacgg acgcttaatc
     4861 cgtgaagaat acatcggcat ccgccctgcg cccggctacc caagctgccc ggaacacacc
     4921 gagaaacaga taatttggga cttgttagag gtggaacaac gcatcggcat gaaactcacc
     4981 gaaagctatg ccatgtggcc ggcagcaagt gtctgcggct ggtacttcac ccaccctacc
     5041 agcaactact tcactctcgg caagattgat gaagatcagg tgttggatta cgccaaacgc
     5101 aaaggctggg acgaactcac ggcgaagaaa tggttaagtg tggcgatgaa acaagatgtc
     5161 aagtcgcagg ctgaaaacat ttaatgtagt ggcgggtttt aacccgcctg ttacaagcgg
     5221 ttggttttga gataaaatat gcaaaattta tctctctttt atgcaaagaa gttatcatcg
     5281 agaacaaaac aatgattgaa aaacaaaatt atcacttagt acaactttta aaagacagta
     5341 attatcaatt aactcaattt tctcctgcgt atataaaaaa attagaggaa tctattttcc
     5401 tcaaatcagg gaaaaatgga gatattcctc atattcaatg tcttgttcgt aaaaaggata
     5461 ttaagctcac acctgaagaa gcagtacgcc aactctattt aatgaatttg cattatgaat
     5521 atgattatcc atttgaacga atggaattag aatattctgt tgtattcggg cgtgagaaga
     5581 aacgagcgga tattgtcatc tttgatagag ataatcctaa cgctgtctat attatggtgg
     5641 agctgaaaaa gccgaaatta aaagatggta aagaacaatt aaaatcttat tgtaatgcga
     5701 ctggagcacc tattggtgta tggacaaatg gcgaaagtat ttcttactat aatcgccgtg
     5761 atcctaatta ttttaaagat attcccgata ttccgaatgc tcaacaaaaa ctttctgatt
     5821 tattacaaga acgttggact attcaagatc tgatagataa tgacaaaatt gccaaacaac
     5881 gtaaatcatt gaaagatctt gttattgaaa tggaagatga ggtactggcg aatgctggag
     5941 ttgatgtttt tgaagagtta tttcaactta tttttactaa actctatgat gaaatggaaa
     6001 gtacacgcaa taaaagtcgt tatttagagt ttcgtaacta tggtgatacc gaaagtgaat
     6061 taaaggcaaa aattcaatct ctatttgatc gtgcaaaaaa taaatgggaa ggcgtatttg
     6121 ctgaaagtgc caaaatcaac ctttcccctt cgcatttggc gatttgtgta tcttcattgg
     6181 aagaagtaaa gctgtttaat tctaaccttg atgtagtgga tgaagcattt gagtacttaa
     6241 ttaataaatc cagtaaagga gagaaaggac aatactttac gcctcgttac gtcattgata
     6301 tgtgcgtaaa aatgctcgat ccaaaacctg aagaaacagt cattgatacc gcagcaggaa
     6361 gctgtggttt tccagttcat tctatttttc acgtttggga aaaacaactt aaagcgagag
     6421 gattagaacg ctcacactta tttactgctg aagaaaaatt gcctgaacaa acagattatg
     6481 tgaaagaaaa agtctttgcg attgatttcg atgaaaaggc agtacgtgtt gctcgtaccc
     6541 taaacttaat cgcaggagat gggcaaacca acgtactaca tttaaatacc ttagattatg
     6601 aacgttggga agattttact aaggaagaag aatggaatga tgtatatgga gaaggctgga
     6661 aaaaattaag aaaattacgt aaaactaaaa atgaaaatcg agatttccaa tttgatgtgc
     6721 taatggcaaa tccgcctttt gccggtgata ttaaagaaac tcgtatatta gctcgctatg
     6781 aattaggcaa aaatagcaaa ggaaaacagc aatccaaagt cggacgagat attttattta
     6841 ttgaacgcaa tttagatttc ttaaaagacg gtggacgaat ggcgattgtg ctaccacaag
     6901 ggcgttttaa caattccagc gataaatata ttcgtgattt tattgcagag cgttgccgca
     6961 ttttagctgt tgtgggctta cacggaaatg tgtttaaacc acataccggc acaaaaactt
     7021 ccgtactatt tgtgcaaaaa tggcacgatg aactttgccc gaaagtagag gattacccga
     7081 ttttctttgc cacaatgcag aaatccagca aagataattc aggcgataaa atctatgtta
     7141 aaaacgctga tggcacactc aaattagaca accatcaaca cctaattgtg gagcacgatc
     7201 tatttaatca cgataatagt acccaagacg gcattgcaga agcctttatt gaatttgcta
     7261 aaaaagaaaa gctctctttt gtgggaaagg attaagccca tttgaccctg ctaaatatca
     7321 ggcattgtta gatgggctag aggtgagcga ggtttatttg agtaaggttc gttctgcaag
     7381 ttattcggaa cgaatggata gtactttctt tttgaaaaaa tacttaaacc atccgagttt
     7441 taattttcca ttatcgctga atgaggtttc tctgattaaa agtggtacaa caccaacaga
     7501 tcgagatgat aacttaaaag agggggttgt cttattaaaa a
//
LOCUS       DANLUA010000123         7457 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLUA010000123 DANLUA010000000
VERSION     DANLUA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            20..2167
                     /locus_tag="PXB44_001871"
     CDS             20..2167
                     /locus_tag="PXB44_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5282325.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2180..3298
                     /locus_tag="PXB44_001872"
     CDS             2180..3298
                     /locus_tag="PXB44_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5282326.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3359..4621)
                     /gene="rho"
                     /locus_tag="PXB44_001873"
     CDS             complement(3359..4621)
                     /gene="rho"
                     /locus_tag="PXB44_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL5282327.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(4807..5754)
                     /gene="cysK"
                     /locus_tag="PXB44_001874"
     CDS             complement(4807..5754)
                     /gene="cysK"
                     /locus_tag="PXB44_001874"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL5282328.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            complement(5928..7424)
                     /gene="pepA"
                     /locus_tag="PXB44_001875"
     CDS             complement(5928..7424)
                     /gene="pepA"
                     /locus_tag="PXB44_001875"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL5282329.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIVDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
BASE COUNT         2137 a         1501 c         1618 g         2201 t
ORIGIN      
        1 aatataagga gaaatcataa tgaaacgctt gcgttattct tatcttgctt tgatattagg
       61 ttcagcctta cctttatcgg cttatgctga cggttattta agtaacgata atattcggca
      121 atttgatgcc cgttttaatt ttttcctcgc aaatccacta cctgcgccta ttgcaaaagc
      181 agatgggcgt ggtgaagcga ttgagaattt ggtggtgaaa tatgcatcag attatcttaa
      241 tgatatctat cttaaagagg atgttattcg ttttcgccgt tggcagtggc aagatctcta
      301 tacgtcattg gatttaaaaa cgaaacaaaa gcccgattat ttaaatactt accgattaca
      361 ggcggaagcg tactttatca ataagcaata ccaagaagca ttatctcaac tggatcaaat
      421 tctgcggcgt aaccctaatg atgttcatgc aatggcaatg agtgtggttg caagtcgggt
      481 attggaaaat cctaatactg aagccgagcg tttattagcg ctttatcaaa tctctccaag
      541 tattgcgcaa aaggtgcagc aatatctaca ttttacagac actatgcttg ctgccagcta
      601 tggtagtgag cctcaaacaa caatgatccc cgacaccata gcggtatttg ggcaatcgcc
      661 taacccagac ggtactccaa gcaagggatt attgcaacgt cttgagaaaa ccaaagaaat
      721 ggcagagaaa tttccgaatg caaaaattat tttaagtggt ggacctgttc gttatatcta
      781 tgcagaagcg gatgtaatga aaaaatggtt gatagagaat ggcgttgatg aaagcagact
      841 gttgcttgat gatgtggcac gagatactcc gggaaatgct gtcggtatca ttgattttat
      901 gaaggaatat ggaggtagaa aagtattggg gatcggtacg atcttacatt taccgagagc
      961 aatgtctgta ttgaaaagct atgcggattc cattggatat gaattggaaa ttgattctgc
     1021 cggaggcggt tctcctccta atgaaaaaaa taaaaaaggt gaagctcttt atacatttgt
     1081 taatgttgct cgtgcaatgg ggttatttac cttgggcgat tttgagaatt tgcttgccaa
     1141 agaacaagct gaaaaagaac aaagagaagc gttggaaaaa gcacagcaga attatttaga
     1201 aaaattggta gctgagcgag atcaagcagt aaaaaatgca gaaaattctg caaatgagct
     1261 ggaagtagcc agacaggcgt tagaaacagc aaaacaggct catcagcaag ctgaagagga
     1321 attgaataaa gaacttagta aaacgcaaga gcagttaaaa cagagtacgg aagcccttag
     1381 tcgtttaata ataaagcaga acaatccttt ttccgagcat tatgttgtac agcttaattt
     1441 gatggaacaa gcaacttatg atctgtaccg taggttagca gaaatagaac atacacaaga
     1501 ttggaattta tggctttcgg atagtctatc caaatatcgt ttaaaaaatc gttcaaccga
     1561 agactatcac ttattacata tcgggctaga tcatcaggtt tcccataatt ggtctattgg
     1621 tggagtattt ggtatcagtc aggggcaaat tgagaatagg acgcataaag tgaaagggac
     1681 atctgctggt atttatgctg gttatcgtcc tacaagtggt ctgttttgga caaatttttt
     1741 taattacagt cattttacta atcaatctgg tgtaacaaaa cgagataata atgcatacag
     1801 tctgagtagt gaaattggaa gaaatatcgt gatgagtcat caatggcaaa ttacgccaaa
     1861 gctgcaatta ggctgggcaa aagctggatc agcacgaatg ttttgggctg ggacagggct
     1921 acgtttagga aaagtgattg aactcaatgc aatcaaactt aaaccttatg ctgatattag
     1981 ctatcgtttt cgaaatcgtc atattgatac cttagatagt ttatctgatc gtcatcggaa
     2041 tgtaatcagt attgggatac aagcagttgg aaatcgccat tattttgcaa tcgaaggatc
     2101 aatgttacac ggggatgcat tgaagcgttc atacggatta cacgcaaatt atcactatca
     2161 atggtaaata ggagcctcta tgaaaaaaat caaaacaacc ctttctgccc ttgctttaag
     2221 cctttctttg ataagtgtaa atagctgggc gataccagct gattatttga gccaagacaa
     2281 tatcagtcaa tatgataagg cgtttaactt tgtattagca aaagtactgc ctgcaccaaa
     2341 aggtaaagaa gatggtcgag gagcgccgat tgagcattta gttattcaat atgcgccaga
     2401 ctatgttgat gacccttatc taaaagagga tgtgctgaat ttccgccgtt ggtattggca
     2461 agcgatgtat gcttcgcttg atcagaaagt taaagagaag ccgacctacc ttaacactta
     2521 ccgtttacaa gcagaggcgt atttcgtcaa taaagagtat cgggaagcac tttctcagct
     2581 cgaccaaatt ttgcgccaaa acccccgtga tattcacgct cttgcgatga cagtattagc
     2641 ttcaagggtg ctagaagata ctgaacaaga aacggctcgt ctttctgcac tgaaacagct
     2701 tgctcccgaa gtcgcacaac gaatgacaga cttttttgcc tttaccgaac aaacgctggc
     2761 ggcaagctac ggtaatgaac cacagacagc aatgatccct gatacgattg ccgtttttgg
     2821 ttctacgcca aacaaagacg gcacaccttc gccgctgttg ttagatcgat tacagaaaac
     2881 caaagagatg gcacaaaaat ttccgcaagc aaaaattatc gtcagtggcg gtgctgtgaa
     2941 aacggaattt gctgaggctg atgtgatggc aaaatggctt agcgaaaatg gtattgaaaa
     3001 agaccgtttg ctacttgatc ctgttgccag agataccccg ggaaatgcgg ttgggattat
     3061 caagctgatg caaacttata atggcaagaa agtattaggt atcggtacgt tacaacatat
     3121 ccctcgtgca acagcggttc tcaaaggtta tgcacagcac attggttatc cgattgagat
     3181 ggattccgtt ggtggtggca agccggcaac accgggaaaa gccaaaatcg aggcacttta
     3241 tacttatgta aatgtggcga gatcaatggg gttgtttgag ttgggggatt ttaagtagtt
     3301 actttattga cagctgctat tgataaaaat cccgcgttaa gcgggatttt tttatgttct
     3361 acgaacgttt cataatctcg aaaaactctt cgttagtttt tgccatcatt aatttgtcga
     3421 ttagccattc gatagcatct acttcgtcca ttgggttgag gactttgcgt aacatccaag
     3481 ttttttggag ttcgtctgct gccattaaga ggtcatcttt acgagtacct gagcggttga
     3541 agtcaatagc cgggaagata cggcgttcag caatacggcg tgagaggtgt aattccatat
     3601 tacccgtacc tttgaactcc tcgaaaatca cttcgtccat ttttgatccg gtatccacaa
     3661 gtgcggttgc gataatggtt aagctgccac cttcttccac attacgggct gcaccgaaga
     3721 aacgttttgg acgatgtaag gcgttagcgt ccacaccacc ggaaaggatt ttgccggaaa
     3781 ccggggtaac ggtgttgtag gcacgggcta aacgagtgat ggagtctaat aaaatcacca
     3841 catctttttt atgctctacc gagcgttttg ctttctcgat taccatctcc gcaacttgaa
     3901 cgtggcgggt tgccggttca tcaaaggttg aggcaattac ttcaccacgt acggtgcgtt
     3961 gcatctctgt tacttcttcc ggacgctcat caattaataa tacgataagc tcacattcag
     4021 gatgattatg ggtgatgctt tgtgcaatat tttgcagtaa cacggtttta ccagcttttg
     4081 gcggggcaac aattaacgct cgctgacctt taccgattgg ggcggctaaa tctaaaatac
     4141 gagcggttaa atcttctttt gatccattgc cacgctccat ttttaagcga gaattggcgt
     4201 gcagaggggt taagttttca aagagaattt tactgcgaga ggcttccggt ttgtcatcat
     4261 taactgaatc aactttcagt agggcaaaat agcgttctcc ctcttttggt gggcgaattt
     4321 taccttctat tttatcgcca gtttgtaagt taaaacggcg gatttggctt ggagaaacat
     4381 aaatatcatc agggccggct aaatatgagc tatctgatga gcggaggaaa ccgaagccgt
     4441 ccggtagaat ttcaagcacg ccttgcccga aaatgtcttc accgcttttt gcgtgttgtt
     4501 taagaatagc aaatacgatg tcttgtttac gtaaacgagc cagattttct aagcccattt
     4561 gattttcacc catttccaca agctttgaaa caggggtgtt tttaagttga gtaagatgca
     4621 taatttgaat gtaaaattgt gattaattaa gattggattt gttgatattg attagatacg
     4681 gccaaccgta aagatgtggc gaagaataac gcaataggag acaaattgca aattttttat
     4741 aaaaaataac cgcttgtttg caataaaaat gccgacatca gccggcattt ttatattaat
     4801 ttaatattac gcttcaatcc cttcaaacag tagcgtactt aaataacgct ctgaggctga
     4861 aggcagaatc actacaatta atttatcggc aaattcaggt agtttagcga tgcgatctgc
     4921 ggcagcaacg gcagcacctg atgaaatacc gcctaaaatg ccttcttctg ccattaaacg
     4981 gcgtgcggtt gtaatcgctt cttcgcttga aacttgttca acacggtcaa gcagtgataa
     5041 atctaagttt ttcggaataa agcctgcacc aatgccttga attttgtgcg ggcccggttt
     5101 gacttcttca ccgtttaaag tttgcgtaat gaccggcgat tcactcggtt ctaccgccac
     5161 tgaaataatt tgcttgcctt tatcttgctt aatagcacgg gaaacaccgg taatagtgcc
     5221 acctgttcct acgcctgcca ctaatacatc aattttacct tcagttgcat cccaaatttc
     5281 aggaccggta gtttgttggt gaattgccgg gtttgccggg ttttcaaact gttttaaggt
     5341 aacatagcga tctgggtcgg aagccgcgat ttcttcagct ttcgcaatcg ctcctttcat
     5401 tccttttgcc ccttcggtta agactaaatt tactcctaag ccacgcaata agcgtttacg
     5461 ctcggtactc attgtttccg gcattgtgag cgttaattta taaccacgag cggctgctac
     5521 ataagccaag gcaattccag tgttaccgct ggttgcatct acaatttctt tgtctttggt
     5581 tagaataccg tctttctctg cttgccacac catattcgca ccaatacggc atttcacgct
     5641 aaaacttggg ttacgccctt ccacttttac cacaatattg ccgttgccaa aattctttaa
     5701 acgcaccagc ggggtattgc cgattgagta tgagttgtct gcatagatag tcatagtgat
     5761 attccttaat aagtattgag attgggattt atcatactta tttttaatcg cttttctagt
     5821 agctggaatt tatttgatat aactaaaaag aatatagggg cgtatgaaga ggatcggtct
     5881 gattttcttt ttgcaaaaac tcgataaaat cagaccgctt gtgagagtta gacctgggct
     5941 ttattgatca aaaattgtgt tagtaaggat accggtctgc cggtcgcccc tttggctgcc
     6001 ccagatttcc aagccgtgcc ggcaatatct agatgcgccc aagtatattt ctttgtaaag
     6061 ttagagagga attgaccggc agtacaggct ccgcctaaac gtccgccgat attagcaaga
     6121 tcagcaaagt tagattttaa ctgttcttga tattcttccc ctaaaggtaa acgccacgct
     6181 ttgtcatcgg cttgctctgc ggcagctaag agttcgtttg ctaaaccgtt atgaggtgac
     6241 atcagcccgc tgttatgatt acctaaggca atcatgcaag caccagttag ggtagcaata
     6301 tcaatcacta attccggttc aaaacgttct acatagctga gagcatcaca tagcactaaa
     6361 cgtccttctg catcggtgtt gagtacctca acggttaaac cagacattgt agtgagaata
     6421 tcccccggtc gataggcttt gccatcaacc gcattttcac agccggcgag taagccgata
     6481 acattgagtg gtaatttcat ctcggcaatt gctttgattg taccgtaaac agtagcagct
     6541 ccgcacatat cgtatttcat ttcgtccatc gcctccgcag gtttaatgga taaaccgcca
     6601 gagtcaaagg tcattccttt gccgactaag ataatcggct tcgcatcagc attcgggttg
     6661 cctttatatt caatcactga aagaaaggcc tcattttcag aggctctgga aaccgctaag
     6721 taagcattca ttcccaattc tgccatctgt ttttcatcaa caatggtagt tgaaatggca
     6781 ggatagtcgt aggctaaacc cttagccaat tcggctaaat agtgaggatt acacacatta
     6841 ggcggacaat ttgctacatt tttagcgtgg ctgattccaa gtgctacggc ttttccgtgt
     6901 tctaaagctc gttcggcatc aggaagatct ttacggtgtg cgacattaaa aatcacacgg
     6961 cgtaattcac ggcgaacttc aggtttgatg cttttgaaat cgttataggc atatagttgt
     7021 tcttggattg cctctaccgc aaatcgcaca ttccaataag gtgttctacc tttaacgtgc
     7081 agttcggtta aaaagctgac agcttccatt gaaccggttt cattaatagt ttgtacactt
     7141 ttttgaataa tttgtttgta ttggcgttca gttaattcac gctcttttcc acagccgact
     7201 aataatactc gatcagcgag tacattcggc acattatgca agagtaaggt ttgccctgct
     7261 ttgccttcta aatctcctct tctcagtaat gtgctgatat agccgttact taatttatca
     7321 agctgttctg cggcagaaca taatcggcgg ggttcataga caccaacaac caaacaggcg
     7381 gttctttgtt tctcaacact gccatttttg acactaaatt ccatttttcc tccttaattt
     7441 ttattgctac agatttt
//
LOCUS       DANLUA010000124         7454 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLUA010000124 DANLUA010000000
VERSION     DANLUA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..496
                     /locus_tag="PXB44_001876"
     CDS             <1..496
                     /locus_tag="PXB44_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5282330.1"
                     /translation="TINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQNG
                     ATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGIIE
                     ELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLILHR
                     RLTV"
     gene            784..2178
                     /gene="fumC"
                     /locus_tag="PXB44_001877"
     CDS             784..2178
                     /gene="fumC"
                     /locus_tag="PXB44_001877"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL5282331.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2305..2415
                     /locus_tag="PXB44_001878"
     CDS             2305..2415
                     /locus_tag="PXB44_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL5282332.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2520..3770
                     /gene="lysA"
                     /locus_tag="PXB44_001879"
     CDS             2520..3770
                     /gene="lysA"
                     /locus_tag="PXB44_001879"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL5282333.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3784..4413
                     /locus_tag="PXB44_001880"
     CDS             3784..4413
                     /locus_tag="PXB44_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL5282334.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4484..5929
                     /gene="tldD"
                     /locus_tag="PXB44_001881"
     CDS             4484..5929
                     /gene="tldD"
                     /locus_tag="PXB44_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL5282335.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            5993..6874
                     /gene="prmA"
                     /locus_tag="PXB44_001882"
     CDS             5993..6874
                     /gene="prmA"
                     /locus_tag="PXB44_001882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL5282336.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7049..7124)
                     /locus_tag="PXB44_001883"
     tRNA            complement(7049..7124)
                     /locus_tag="PXB44_001883"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7089..7091),aa:Trp,seq:cca)
     gene            complement(7190..7266)
                     /locus_tag="PXB44_001884"
     tRNA            complement(7190..7266)
                     /locus_tag="PXB44_001884"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7230..7232),aa:Asp,seq:gtc)
     gene            complement(7277..7392)
                     /gene="rrf"
                     /locus_tag="PXB44_001885"
     rRNA            complement(7277..7392)
                     /gene="rrf"
                     /locus_tag="PXB44_001885"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2222 a         1662 c         1645 g         1925 t
ORIGIN      
        1 aacaatcaat gaaattaaag aagaaatcta ccctatattt gagcctatca ttggaacaat
       61 tcggctaaaa acctactttt cctactacgc agtttttaag gatggattga tgattgcatt
      121 gtatcaaaat ggagcaactt atttacgtat tgcgaaaaaa gatattgaat acattcgtca
      181 gtatcctaaa acctgtatct tatgcgatga aaaaattggt atacaaagta gaaaatttca
      241 ttatattccg caagttctca tagatgatac accaaaattt agctcgctac tctttggaat
      301 tatagaggaa ctccgaacgg agaagtacaa aataagccaa aagcgagcta cacaaatccg
      361 aaacctacca aatatgaatt taaagttaga aagaatgttg aaaaaaatag ggattcattc
      421 cattcatgat tttactcaag aagggtatat gtcaactttt atcaaactga ttctccaccg
      481 aagacttact gtttaagcta aacggagctt taaatcacca atatatttac accttcacag
      541 atcaacaaaa aagggaacta atgcaagaag caaatcaagc gctatataat tcaggcctaa
      601 gaaaaagatt taacgccctg tagttaaata atgtgatcca aatcataaaa tataaaaaaa
      661 ccaataagga attgcaattc gcagttcctt tctttttctt ttatatgcta gtatcacctt
      721 atacctctaa attattagtg cctactgatt ttcaattaat ccaacaattt aggagagcgc
      781 atcatggaat tccgtatcga aaaagacaca atgggcgaag tgcaagttcc tgccgataaa
      841 tactgggcgg cgcaaaccga gcgttcacgc aataacttca aaatcgggcc ggaggcatca
      901 atgccggcgg aaattatcga ggcgtttggc tatctgaaaa aagcggctgc ctatgctaat
      961 cacgatttag gcgtactagc ggtcgaaaaa cgggatttaa ttgcaaaagc ctgtgatgaa
     1021 attttggcga acaaactcga tgatcaattc ccgctggtga tttggcaaac cggctcgggt
     1081 acgcagtcaa atatgaacgt gaatgaagtc gtggcaaacc gtgcacacgt gttaaacggc
     1141 ggcaagctgg gtgaaaaatc gattattcac ccgaatgatg atgtgaataa atcccaatct
     1201 tctaacgaca cctacccaac tgcaatgcac atcgctgctt acaaaaaagt ggtggaacac
     1261 accctacctt gtgtgaaacg tctgcaaaaa accttagcgg aaaaagccga agcctttaaa
     1321 gatgtggtca aaatagggcg tactcactta atggatgcta ccccgctcac gctcggtcaa
     1381 gagttctctg cctatgcagc acagttagca ttcggcatca aagcattgga aaacaccctg
     1441 ccacacctgc gtgagctggc actcggcggc acagcagtcg gcaccggctt gaataccccg
     1501 caaggttacg atgtgaaagt ggcggaatac attgcgaaat tcaccggttt accgttcgtt
     1561 accgcccaaa acaaatttga ggcattagcg actcacgatg cgattgtcga aacccacggt
     1621 gcattaaaac agcttgcggt gtcgctctac aaaattgcca acgatgtccg tttattagcc
     1681 tccggtcctc gttcaggcat tggcgaaatt ctgattccgg aaaacgagcc gggttcatca
     1741 attatgccgg gtaaagtaaa cccaacccaa tgcgaagcaa tgacaatggt atgtgcccaa
     1801 gtattaggca acgatgtaac catctctttt gccggcacgc aagggcattt ccaactgaat
     1861 gtctataaac cggtgatggc gtacaacttc ctgcaatcgg ctcaactgct aggcgatgcg
     1921 tgcgtgtcgt ttgacgaaca ctgtgcagtc ggcattgaac cgaactaccc tcgcattaaa
     1981 caacagctcg accaatcgct gatgttagtg accgcattaa atactcacat tggttatgaa
     2041 aatgcagcga aaatcgccaa aactgcccac aaaaacggca caacactgaa agaagaggcg
     2101 attaacttag gcttagtcac ggcagaacag ttcgatgaat gggttcgtcc ggaagatatg
     2161 gttggcagct tgaaataagc ccaacccttc tcttgtaatc tgatttacac ctcaatctac
     2221 cggtaagatt gaggtgtaat tttttatagc gagggtactg attttgtttt agaatgaagc
     2281 atcatttttt aagcggaaaa acctatgaaa aaactcattt taatcgcatt agttactact
     2341 ttaggcttaa gtgcttgtgg ggtcaaaggc ccactctact tccctgagca agcccctaaa
     2401 catgaacaaa aataagcatt gcaagcggtc tgttttggct aaaattttgc aaatttttgt
     2461 gaaaaaatga ccgcttgttg cctcttttct ttcaaaatcc caaacacaac ggactttcaa
     2521 tgaaccattt tgaatataaa aacaaccaac tttttgctga agaagtcgct attgccgata
     2581 ttatcgagca atacggcacg ccagcttatg tttactccag atctaccctt gaacgccact
     2641 ggcacgcatt caataatgcg tttggctcgc acccacattt agtttgcttt gcagtcaaat
     2701 ccaactctaa cattgccttg ttaaatgtaa tggctcgtct cggttcaggc tttgatattg
     2761 tctctcaagg cgagttggag cgtgtcttag ctgccggcgg cgaagcctct aaagttgtct
     2821 tttccggtgt ggcaaaatcg cacagcgaaa tcaaacgagc attggaagtc gggattcgtt
     2881 gctttaacat tgaatcggtt tccgaacttc accgcattaa ccaagttgcc ggtgaactag
     2941 gcaaaatcgc accgatttca ctgcgggtaa acccggatgt ggacgcccac actcacccat
     3001 acatttccac cggtttaaaa gaaaacaaat tcggcgtgag cgttactcaa gcccgtgaag
     3061 tgtatcgctt agccaaaacc ttaccgaatg tgaaaattac cggtatggat tgtcacatcg
     3121 gttcgcaatt aaccgaattg caaccttttt tagatgcaac ggatcgctta atcatcttaa
     3181 tggagcaact gcgtgaagac ggcattgagc tacaccactt agacttaggt ggcggtttag
     3241 gcgtgcctta caacggcgaa gagcctccgc atccgaccga atatgccaaa gccttgctgg
     3301 aaaagctcaa aggctatgaa aacttagaaa tcattattga accggggcgt gccattaccg
     3361 caaatgccgg tattttagtg acgaaggtgg aatacttgaa acaaaatgaa gatcgtaatt
     3421 ttgcgattgt cgataccggt atgaacgata tgattcgccc ggctctgtat gaagcctata
     3481 tgcaaattac ggaagtggat aaaaccttaa accgtgacaa agctgtttac gatgtggtcg
     3541 gtccgatttg cgaaacctcg gatttcttag gcaaacaaag agaattggca atttctgaag
     3601 gggatttaat cgctattcgt tctgccggtg cttatggggc ggcgatgtct tcaacctaca
     3661 actctcgccc acaagcggca gaaattatgg ttgatggcaa ccaagcccat ttaattaaag
     3721 cccgagccac ttttgctgat ttatggcgtt tagaaaaatt attaccttaa tgaggaagaa
     3781 aagatgaaat tatatggatt aaccggtgct tgttcatttg taccacacgt tgccctagag
     3841 tgggtgaaat tgcgtgccaa tcaagattat gcgtttcaag ctgttagccg tgaatttatt
     3901 aaatcggcgg aatatctggc tttaaaccct cgtggtaacg taccattgtt ggttgatggc
     3961 gacttggccc tcacgcaaaa ccaagctatc gtccactatt tagacgagct ttatccggaa
     4021 gctaaattat tcggcagcaa aaccgctcgt gataaagcca aagcggctcg ttggttagct
     4081 tttttcaatt cagatgtgca taaatcattc gtgccactgt tccgcttgcc aagttatgcg
     4141 gaaggcaatg aaacgctcac caaaactatt cgccaacaat cggctgagca aattttagaa
     4201 caactggctt ttgcgaatgc tcatttagaa aatcatattt tctttggcga agaaatttcg
     4261 gttgccgatg cgtatctcta cattatgctc aactggtgtc gcctactagg gttggatttc
     4321 tctcatcttt cccaactttc tgcctttatg caacgagtgg aggcagacca aggcgtagat
     4381 aatgtgcgtg agcaagaagg cttaaaaggt taattcacca ttgttcattt aagacggtag
     4441 attttatgtt aaaatctacc gtcttttatt tttaggaatc accatgctaa atcaggtttc
     4501 aaacagcttg cttgctccaa gtgggcttga attatcgcac ttaactcaag tgttagatca
     4561 cttctccggc cgtcatattg attatgccga tctctacttc caactcagcc aagacgaaag
     4621 ctggagcttg gaagacagca tcatcaaaga aggcggtttt tatatcgacc gagggttcgg
     4681 tgtgcgagcc atatcaggcg aaaaaaccgg ctttgcctac gccgatcaaa tcacgttaaa
     4741 acagcttgag caatgtgccg ctgccgcacg ttcaatcagc ccaacaagcg gtcaattatc
     4801 ggtaaaaaat ttcaaaaaaa cgaccgcttg ttctcgctac ccggcaatca acccgctaga
     4861 gaccatcagc cgtgaacaaa aggtcgaatt gctccatttg gtggataaaa ccgctcgaat
     4921 ggcagatccg agagtgattc aagtgaatgc gaatctctca gcggtatatg aagaaatgtt
     4981 agtggcagcg actgacggca cactggctgc ggatattcgc ccgttagtgc gtttatctgt
     5041 gtcggtactg gtggaacaaa atggcaagcg tgaacgaggt tcagcaggtg ccggtggtcg
     5101 ctttggttta gattggttct tagcaccaag cgaaaccggc tttagccgtg cggaatcgct
     5161 taccaaagaa gccgttcgcc aagccctcgt taatctcaca gccattcctg caccggcagg
     5221 cacgatgccg attgttctcg gtgctggctg gccgggcatt ttgctccacg aagcagtcgg
     5281 acacggcttg gagggtgatt ttaaccgtaa ggaatcttcg ctcttcagtg gtaaaatcgg
     5341 gcaaatggtc acctctgagc tttgcaccat tgttgatgac ggtacggtgc cgaatatgcg
     5401 aggctcaatc acggtagatg acgaaggcgt gccttcgcaa cgtaatgtgt taattgaaaa
     5461 cggcattcta aaaggctata tgcaagacaa actcaatgcc cgcctgatgg gagttgcccc
     5521 aaccggcaac ggcagacggg aatcttacgc tcatttgccg atgccacgaa tgaccaacac
     5581 ctacctcacc gaaggcaatc acgaatttga agagatgatt gaatcggtcg aatacggttt
     5641 atatgctcct cacttcagtg gtggacaagt ggatattacc tccggcaaat ttgtgttctc
     5701 caccgccgaa gcctacttaa tcgaaaaagg caaaatcacc aaacctgtta ccggtgcaac
     5761 cctgatcggc agcggcatag aagcaatgca acaagtttca atggtcggca aaaaaatgga
     5821 actcgacccc ggcatcggca cttgtggcaa agaagggcaa tccgtaccag tcggcgtagg
     5881 gcagcctaca gtgaagttgg ataaaattac ggtgggggga agagggtaat ctctccccgt
     5941 ttgcaaaata taaagaaaat atcaccgctt gtagcgataa aaggaaaaca aaatggcttg
     6001 ggtacaaatt cgtttaaaca gtacagataa acaggctgag gaaatcagcg attttttaac
     6061 cgagattgga gcggtgtcag tgacttttat ggatagtcaa gataccccaa tttttgaacc
     6121 tcttccgggg gaaacccgtc tttggggaaa taccgatgtg gttgggcttt tcgatgcgga
     6181 aactgatatg aatcttatcg tagaagcact aaaaaccagc aaattggtgg acgaaaactt
     6241 tgctcacaaa atcgagcaaa tcgaagacaa agactgggaa cgggagtgga tggataattt
     6301 ccacccaatg cagtttggca aacggttatg gatctgccca agctggcgtg aagtaccaga
     6361 cccgaatgca gtgaatgtta tgttagaccc tggtttagca tttggcaccg gcacacaccc
     6421 aacgacctcg ctttgcttac aatggttaga tagcttagat ttagaaggca aaacggttat
     6481 cgacttcggc tgtggctccg gtattttagc tatcgctgca cttaaactcg gtgcaaaaca
     6541 agccatcggg attgatatcg acccacaggc gattttagct agtcaaaaca atgctgaggc
     6601 gaacggcgta gcagaaaaac tacaactatt cttagccaaa gatcagccaa gagagttaca
     6661 agccgatgtc gttgttgcga atattctagc cggcccactc aaagagttag caccgaatat
     6721 catcacgctg gttaaaccaa acggcaattt aggcttatcc ggcattcttg ctactcaggc
     6781 tgaaagtgtg tgtgaggctt atagcgaagc ctttacgcta gaccctgttg tagaaaaaga
     6841 agaatggtgt cgtattacag gccgcaaaat ctaatataag aagccctgtt ttaaacaggg
     6901 cttacttttt gataaattct actactcttt acctgtcttg ggaatcgttc tccctaaatt
     6961 aattaacaca agcggtagaa ataacaggaa aatttacaaa ttacataaat gaaaaaagcc
     7021 gactgaaatc agtcggcttt ctaaataatg gcaggggcgg agaggctcga actcccaaca
     7081 cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctattgat aggtattcat
     7141 tttgcactta taggggcgaa ttactattcg cccctgcatt aaagattatt ggcggaacgg
     7201 acgggactcg aacccgcgac cccctgcgtg acaggcaggt attctaacca gctgaactac
     7261 cgctcctaaa ttaatagatt gataatgccc tactctcaca tggcgaatca ccacactacc
     7321 atcggcgtta ctgcgtttta cttctgagtt cggtatggag tcaggtagag ccacagcact
     7381 ctggttatca atcaaattct ttcatttagt cagtttacta aaatatagtt tatactttct
     7441 aataaactca ctaa
//
LOCUS       DANLUA010000125         7433 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLUA010000125 DANLUA010000000
VERSION     DANLUA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(43..1626)
                     /locus_tag="PXB44_001886"
     CDS             complement(43..1626)
                     /locus_tag="PXB44_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282337.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            1848..3005
                     /gene="hemW"
                     /locus_tag="PXB44_001887"
     CDS             1848..3005
                     /gene="hemW"
                     /locus_tag="PXB44_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL5282338.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            3148..3630
                     /locus_tag="PXB44_001888"
     CDS             3148..3630
                     /locus_tag="PXB44_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5282339.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            3599..3796
                     /locus_tag="PXB44_001889"
     CDS             3599..3796
                     /locus_tag="PXB44_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282340.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            3883..4350
                     /gene="bcp"
                     /locus_tag="PXB44_001890"
     CDS             3883..4350
                     /gene="bcp"
                     /locus_tag="PXB44_001890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL5282341.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGGEQVFDKFKTGEHHQMIVDYLRGR"
     gene            4350..4898
                     /gene="gmhB"
                     /locus_tag="PXB44_001891"
     CDS             4350..4898
                     /gene="gmhB"
                     /locus_tag="PXB44_001891"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL5282342.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            4903..5742
                     /gene="queF"
                     /locus_tag="PXB44_001892"
     CDS             4903..5742
                     /gene="queF"
                     /locus_tag="PXB44_001892"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL5282343.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            5934..7067
                     /locus_tag="PXB44_001893"
     CDS             5934..7067
                     /locus_tag="PXB44_001893"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL5282344.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(7064..>7433)
                     /locus_tag="PXB44_001894"
     CDS             complement(7064..>7433)
                     /locus_tag="PXB44_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5282345.1"
                     /translation="LDKPELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIYIAPILI
                     ILQIMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGLMFGSSK
                     LILAITTLGVMNVLPYIFLI"
BASE COUNT         2263 a         1425 c         1606 g         2139 t
ORIGIN      
        1 ttttgtaaaa tattaccgct tgcatttagc tagccgttac gactatttag cacgaccaac
       61 accgataaat gacatacctg ttgttggtaa tggttggaaa cctgttaatg ccttatcatt
      121 ccacgcagtt ggcaatttac ggtgaataat tgggtaaaga ggcacttgtt cagcgataat
      181 attgattgct ttcgcccagg cttctttaga agcagcagga tttttggccg cttcatcaag
      241 cagtttttgt acttctgcat attcagctgt ttcagaccag cggaaacgac gtttcggcca
      301 tacatcacca cgataccacc agcttaataa taggtctaag tcattaccga aaactgatgg
      361 gtcgccaggt gcaatcacaa cttcgtatgc acctttatca acgtgtgtgc catataatgc
      421 acctgattgt aagtgtttga gtgaaacttt cacgcctttt aagctgttcc acgattcaag
      481 aattaatggc gcacattctt tcacccaaga gtgatcggtt gccaacaatt caaattttaa
      541 ctctgtcaca cccgcttctg ctaataacgc ttcggctttc gctttatcga aatcatattg
      601 cgaatttgct ttcacataat ccgggtgagt atcttgcacg taagatgtag cagcttttgc
      661 attgcctaag aacaccacat caattaattt ttgggtatct aagccataat gtaacgcttg
      721 gcgaactttc gggttgttaa acggtgcttt ttcacagtta aacattaaga acagtaagcc
      781 gaatgattgt actgactcaa cggtaccttt acgttttaag cgttctgcat ctaagtaagg
      841 cacagactca attgcttgaa cacggccaga ttcttgagcg gttacacgag cggcatcatc
      901 agaaagtaag aaccaagtca ttttctcgac ttgtgccggg tagccaccgt tataatctgc
      961 aaatgcttcg aaaacaatac gatcatcttt agttgcggat acaaatttat atgggccagt
     1021 ccccactgga ttagcatcaa atgcagcttg accggcttct accactgctt ttggtacgat
     1081 tttaacaatg gttaggcgct ctttgaataa cgcaaatgga tatttcaatt tgaattcaac
     1141 cactttgtca tcaacttttt tcacagtgtc aataaatgga atgaattgtg cgaaaagtga
     1201 tgctttagct gggtctaata cacgttcaaa tgagtaaact acgtcttcag tggtaaccgg
     1261 attaccattg tggaatttag ccccgtcacg taaagtaatt cgataggtta catcatcaat
     1321 tttttcaggc tctgctgctg ccaatgctaa atatggtttg cgagtggctg gatgtaagtc
     1381 cacgaggcct tcaaaaatat ggaggtttgc cgccattgag gatgcaccgc ttgaggtaag
     1441 cgggtcaaat ccggttgata tgggataagc tataccggcc tcaatcgttg agccggcggc
     1501 aggggctgca aatgctttag gtgtaaatgt acctaaagtg ccactaaatg ccataccggc
     1561 accgacacct gcaattagtt tcataaaacc acggcgggat tcgttgtgtt caaactgttt
     1621 tttcatctta aagctccttt aagtttttat gataggggcg gtattatttg ctgaagtgaa
     1681 tttatcaaaa aatgcacttt tttgaattga atttatcata tcattaatgt caaagtggta
     1741 aagcaagaat tattgaattt gtgatctagc tcacgaaaat ctttgctttt cacttcacgt
     1801 gcttttcgtt attattcgcc aatttaatca taaatgaaaa attgacagtg aacctgattt
     1861 taccgccttt aagcctttat atccatatcc cttggtgcgt gcaaaaatgc ccttattgcg
     1921 attttaactc gcacgctcaa aaaggattaa ttcccgagca agaatatatc gaacatttac
     1981 ttgcagattt atcccaagat ttgaccgctt accgagtggc aataggtgat cgtaaattat
     2041 attctatttt tattggcggc ggcaccccga gcttattttc agcggaaggg atagcttatt
     2101 tattaattga agtagaaaaa cggattgcat tcgagccaaa tatcgaaatt acactggaag
     2161 cgaacccggg aaccgcagag gcagagcgat ttttaggtta cgcacaaggt ggcgtaaccc
     2221 gcatttcaat gggaatccaa agttttgagc cggaaaaatt attgaaatta ggacggattc
     2281 acgatgcaaa tgaagcaaaa caagcgatta aatttgccca aaattctgca aaatcgggct
     2341 tgcaaagttt caacattgat ttaatgcacg gcttaccaaa ccaatcggtt aaacaagcct
     2401 tggcggattt agaaacaggg atttcactca atccaccgca tttatcgtgg tatcagctca
     2461 cgattgagcc gaatacgatg ttctattatc gtaagccaac cttgcctgat gatgacgagt
     2521 tatgggatat tttcgagcaa gggcatcaac ttttaacgaa tgccggctat gagcagtatg
     2581 aaacctcggc ttatgccaaa aaaggctacc aatgcagaca caatttaaat tattggcgat
     2641 ttggggatta tttggcgatt ggttgtggcg ctcacggcaa aatttcatat ccgaccgggg
     2701 aaatttatcg ctttagcaaa atcaaacacc cgaaaggtta tatgcgaggt gaatatcgct
     2761 acagccaaga tttgattgaa ttagacgacc gcccgtttga gttttttatg aaccgcttta
     2821 gattacttga agcgacacca aaacaagaat ttgaatggtt tacagggtta gacagggaag
     2881 ttgttcgccc gacaatcgat tgggcgttaa accaaaacta tatttccgaa accgcaaccc
     2941 attggcaaat tacccaacac ggtaaattgt ttttaaatga attactggaa ggatttttgg
     3001 aataatttgc aaaaaaacga ctaaaaaaag accgcattgt aatgctaaat tacaggcgtt
     3061 ttttgtgatc tgcgtcgaaa aatcctcatt ttttactaga caaaaaaaca gtgtttagta
     3121 gcatagcctt cacaataagg agagcttatg atcgataaac aaatccgtaa tagtaccgcc
     3181 gaatttttga tttttactca acaaaatggg gcagaaaata ttaccgttcg ctatgaagat
     3241 aacgaaattt ggcttacgca aaaattaatt gctcagcttt ttgattgctc aacagataat
     3301 gtttctttgc atttaaaaaa catttttaat agtagggaac tcgatccaaa ttcagttacc
     3361 gaggatttct cggtaactgc cgcagatgtc aaaaattatc gtaccaaaca ctacaacttt
     3421 gatgccgtta tttcgatagg ctatagggta aactcggtgc gggcgacaca atttcgccaa
     3481 tgggcaacta aagtattacg gaatttcgcc caaaaaggct atgtgattga ccgccaaaga
     3541 atggaaaatg gtacattttt aaatgaagat tattttgagc agttgctggc agaaattcgt
     3601 gaaatttggt tatctgaacg gcgttcttga gggcaaaggc aaaatgcgtt ttgatgaagc
     3661 gaaattgcac gcagaaagcg aatttgagaa ataccgcatt gttcaagaca gattatttga
     3721 aagcgatttt gacaaggtat tgaaagaaat gttgcgttcg gaaaatcaat accgtccatc
     3781 attttcaaca acataaaaca accgcatttt gcaaattttt tgccaagttt gaccgcttgc
     3841 cgtgtataat gcaagcggtt attttttatg gaaaaattac aaatgaatat cttacaagca
     3901 ggcgataaag cccctaaatt tacccttcag aatcaggctg acgagccggt ttctctctct
     3961 caatttgccg gcaaaaaagt actggtttat ttttatccga aagctcttac acccggttgc
     4021 actacgcaag cgtgcggact gcgtgacagc aaatcagaac ttgatgagct caatgtggtt
     4081 gttctcggaa ttagcaccga tttaccgaaa aaattagctc aatttgtaga gaaaaaagca
     4141 cttaatttta ccttattatc tgacccggat caccaagttg cagaagcctt tggtgtatgg
     4201 ggcgaaaaga aatttatggg aaaaacctat gacggcattc atcgcatcag ttttttaatt
     4261 gatgagcagg gggggggtga gcaagtgttt gataaattta aaaccggcga acatcaccaa
     4321 atgatcgtgg attatctacg gggtcgctaa tgggaaaaaa agccattttt ttagaccgtg
     4381 acggcacaat taatatcgat cacggctatg tgcatcaaat tgatgatttt caatttattg
     4441 acggtgtcgg caaggcgtta aaacagctac aagacaaagg ttatttattg gtgctggtaa
     4501 ccaatcaatc tggcattgcc cgaggctatt ttagcgaagc acaatttatg caactaaccg
     4561 aatggatgga ctggtcgctc gatgaagatt atggcgtggt actagacggt atttactact
     4621 gcccacatca ccccgaaggc aaaggcgaat accgtaaaga ttgtgattgc cgtaagccta
     4681 aagcaggtat gtttactcaa gcgattcaag atttaaacat cgacccaagc caatcttata
     4741 tggtgggcga taaattagaa gatatccttg ccgcagaagc cgccggcgtg aaaaccaaag
     4801 tgctggttcg cactgacaaa gcagtgacgg aagagggtca ggctaaagcc gatttggtgt
     4861 tagatagttt agttgattta gtaaaatata ttaaataaag caatgaatta taacgataaa
     4921 tccctttccg ccctgaaatt agggcaaaaa accgagtaca aaagcgaata tgatgcctcg
     4981 cttttgcaac ctgttcctcg taaattaaac cgtgatagtt taggcattac acaagagcag
     5041 ccttttaacc gaggggctga tgtgtggact tgctatgagc tatcttggtt gaaccaaaat
     5101 gggctaccgc aagtagcgat tgcagatgtc gccattgatt ttaagagcga aaacttaatt
     5161 gaatcgaaaa gtttcaagct ctatttgaac agttttaacc aaaccaaatt tagctcaata
     5221 gaagaagttg ggaaaacgat tcaagcggat ctggctcgct gtgcaagcgg tcaggtttcg
     5281 gtaaaaattt accaattatc acactacacg caacagccga tagttgattt tgcaggcgat
     5341 tgcattgatg agcaagatat tcaaatcgac agttatgcgt tttcgactca ttatttagag
     5401 aacattgcag aagatgagtt ggtggaagaa accttagtca gccatttgct caaatctaac
     5461 tgcttaatta cgtcacagcc tgactgggga agtgtgcaaa ttcgctacaa aggcaaaaag
     5521 ctaaaccgtg aaaaactgtt acgttattta gtgtcgttcc gtgagcataa tgagttccac
     5581 gaacaatgtg tggaacgcat tttctgtgat ttaatgcaat ttgccgagcc ggaaaaatta
     5641 acagtttacg cacgctacac cagacgaggc gggctagaca tcaatccgtt tagatcgaat
     5701 tttgaggaag tgccggaaaa tctaagaatg gcacgccaat gattttgttg ttttttgttc
     5761 ccgtctgaac cgcctgagcg tgtttgaatt ggtaggtaaa tttactctga gcggagcgaa
     5821 tgtaaatttg ccgttaagcc aattcaaact aagcgaggat agcggtgaag ctcgggttgc
     5881 ctttcttttg gttacttttc tttggcaaag caaagaaaag taacaaatag cagatgttga
     5941 tttttatgat gaatgaatat agcccaaatt actatatagg cgtaatgtcc ggcacgagcc
     6001 ttgatggggt ggatattgct ttgctggatt ttgcaaaaaa tccaccgaaa atgaccgctt
     6061 gtgatttttt tccaatgccg gaggaattac gggcggatat ttctgcgtta cttaaaaccg
     6121 gcgaaaccaa tctccaaaaa ctgggggaaa ttgatcaccg tttaggcttg ctttatgcag
     6181 aaacgatcaa tgcgttttta gcgaaaaatc agctaaaagc agaagatatt caagccatag
     6241 gttgtcacgg gcagacggtg tggcattcac cggattctga gtatcccttc accacccaaa
     6301 ttggcgatat gaatttggtg gcagagcaaa ccggtattac cactatcgct gattttcggc
     6361 gtaaagatat ggcagtaggt gggcaagggg ctccgcttgt gccggcattt catcaagcta
     6421 tttttagcga tccaaatcgt ctaacagtgg tgctgaatat tggcggcatc agcaatattt
     6481 cggtgttaaa tcccaattcc cctacagttg gttatgatgt aggagccgga aatactttga
     6541 tggatagttg gatcgaacaa catcaaggca aaagatacga taaaaacggc gaatgggcga
     6601 gaacgggcaa gctcaatact gaactgttag cgacgttatt agacgagtcg ttttttgcta
     6661 aagccccgcc taaaagcact gggcgggagc tgtttaattt aacgtggctt gcaaaaaaat
     6721 tggaaaattt gaccgcttgt cagccacaag atgtgcaacg cacgctggca gaatttactg
     6781 ctcaaagtat tgccaatgag ttattacgct ttgaaagctc tctgcctcgc ttgcttttag
     6841 tttgtggtgg tgggtcaaga aatccgttat taatggaacg gatttctgct ttacttcccg
     6901 agtgggaggt tacaaccact acagaatttg gtttagatat tgattatgta gaggctgccg
     6961 cttttgcttg gttagcttac cagcgaatcc ataatttacc cagtaatatg cctagcgtga
     7021 caggagcaaa aaaagccgtg agtttgggcg tgatttaccc tgcttagatt aagaaaatgt
     7081 aaggtaatac attcattact ccgagagtgg taatagctaa aattaattta gagcttccaa
     7141 acattaagcc ttggttttta ccattgtcta atttcactag catattagat agtgccgtat
     7201 tcgcatacac ggtaatagtg gcaaaaatca ctaaatagag aaatgcagtg atggttgaaa
     7261 gccattggag tgcataaaac caaggcatta tttgaagtat aatcagtatt ggtgcaatat
     7321 aaataagttt atgtactgtc agatttaatt ttcgtgaaaa atcagattgc aataacactg
     7381 caattaaatt attcacaaag aatgagacag agactaattc tggtttatcc aga
//
LOCUS       DANLUA010000126         7430 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLUA010000126 DANLUA010000000
VERSION     DANLUA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..403)
                     /locus_tag="PXB44_001895"
     CDS             complement(<1..403)
                     /locus_tag="PXB44_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5282346.1"
                     /translation="MKHDLDLLKYIWDNRNLNNKAYQYLFHICIEPMRKGDWNRYTIW
                     LNNNGNLVNISAVVYEIVSSQQLKDDIKLWQDMQFHCRPVKVETTGFISFLLHQFFNG
                     ITHVLLEQGMITETEAAEKSYHFIAANSFQRL"
     gene            complement(519..1361)
                     /locus_tag="PXB44_001896"
     CDS             complement(519..1361)
                     /locus_tag="PXB44_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282347.1"
                     /translation="MSGSVLNTYFKHALVKAGFPNCLNIEYSLSHCQGDGVAFYGRLN
                     TETLINLFNKLNPQKRTQKMFSNLLNHIESWEPHYGVDFEIIRNSFGYRYSHCNTMEL
                     SVRTADDLAFFYDRDARKEWYFPTSKVEKYKALWDGFIQDLGQYIKDTSCALESNGYQ
                     IIEASPYQTETIFKFNTENYLIELKRKSSEFYSESVDWLCGDVCDFEAMIEEILQGKL
                     CYADFVATVTDKESGIQLGSDEITGVSYSPTDHSLSGYRQELISNAIASARSNAARFA
                     YLHA"
     gene            complement(1439..1834)
                     /locus_tag="PXB44_001897"
     CDS             complement(1439..1834)
                     /locus_tag="PXB44_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282348.1"
                     /translation="MARKKIQYTETDLHLYSVLWNLRHAHDLFSVKLERETKFGKAFS
                     VFSRFDVCPSYGEVDITYLVVKAGIRKTLRGFAYVGGNGFSHSRHLMSTFMYLVREIL
                     VEHNWISETELYDQPISVYKFRAINFDKW"
     gene            complement(1933..2178)
                     /locus_tag="PXB44_001898"
     CDS             complement(1933..2178)
                     /locus_tag="PXB44_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5282349.1"
                     /translation="MLVHIRLSCIGDVLPTKLNEWTSEHQALQQEISHRALEAWQNRD
                     KDIKSVRFMQQMPSRQGLNFRFLSVSEQNGKLIVSRA"
     gene            complement(2250..2603)
                     /locus_tag="PXB44_001899"
     CDS             complement(2250..2603)
                     /locus_tag="PXB44_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282350.1"
                     /translation="MSQQTAGWDVIPFESSKTVKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFWTLLAAIIAF
                     VCGMAAVFGTQLSLI"
     gene            2977..3420
                     /locus_tag="PXB44_001900"
     CDS             2977..3420
                     /locus_tag="PXB44_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL5282351.1"
                     /translation="MKVSRFLGYCSIVTGVFSPLAQAQPNIIVYTTKMYPIQNSQLAS
                     QIYYLDQVENFEESISRNLSKSPEIAEQEMRAFFNSADWKAQEQQLKDAYQGVISGWQ
                     NGIKKVPAVFFDNPQSSPSVIYGTTDVQKALDEWHKWVKQQGESK"
     gene            3417..4358
                     /locus_tag="PXB44_001901"
     CDS             3417..4358
                     /locus_tag="PXB44_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL5282352.1"
                     /translation="MKKTFLTILISASLAVPTTLYPQPARASLNTASIAASSASTSCL
                     EYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNHDGQTPWTELQALNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGNTGYYCKTQTTPFMPYFLSGLDFFA
                     WRENMPEMFYPEALTPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     SRTAQPHIYFPAAKTGGAGEWPPGPVEEGQARTHKWQILYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            4376..6388
                     /locus_tag="PXB44_001902"
     CDS             4376..6388
                     /locus_tag="PXB44_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5282353.1"
                     /translation="MKSHFNFKKSHLRITTALLSSVSGLSYADSITDKLEFSKSDSII
                     SDRVFYQIGGGMGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMVLQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSSQPDAKQAQKDLEDKKGKDGRVWIGGQKKGGENQ
                     EAINVVGDVVKAGFNLLNKRNALDTSTISGSSCDGNLCQTWDNPQTAADFLTKIIGEQ
                     SISTCKEDCGTKTSSKAGVGLSPEIEEEHVRTIVKLENVLNMDVPKAELLADLSSTTV
                     PVTRGLIESLRDDPDVEVLSQRLSAEIATSKIMEKMLLARRAVLAGMREPNVAQNADA
                     QVELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLD
                     KRVQGLENGNVALTDLEAGSNINLSRRNIALSIPNIPNVAALIPNLPNRGGSGVGGNF
                     PSLAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTIVKVTKDT
                     VITKEDAERDLARRTAIFANNVRKELGDSNWNALPPNAQAVLVSYAYNYGSLAKTKSV
                     LDAARRSAQSGDMNALATAIRNRQVDNNGINARRRNQEADYILGKN"
     gene            6409..6837
                     /locus_tag="PXB44_001903"
     CDS             6409..6837
                     /locus_tag="PXB44_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282354.1"
                     /translation="MRKLLGITVLTLCTTSAFATDKNVVFSCTSTEGKPLTVKRVGND
                     YEYSYDKTIFKNPIKKAVTNDGSIIARGSGFTTYALELKNDGLKYVVGFVQPNGNSKE
                     FIEPGATISRDNDQPSIGSVDCDPYKKIYYKFDVHLMNTL"
     gene            7053..>7430
                     /locus_tag="PXB44_001904"
     CDS             7053..>7430
                     /locus_tag="PXB44_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282355.1"
                     /translation="MDITTQNIELRYLSEQFYLDVAPHLKEILLKETRPYCVLLVRVK
                     NLDFALPLRSNLPEREGVGIKTIPNPQEYGKFKGIDFSKAILLTDKSYLKTDTVQLKN
                     KSELLNIQGNERRIVREFKKYVSE"
BASE COUNT         2390 a         1425 c         1487 g         2128 t
ORIGIN      
        1 gtaaacgttg aaagctatta gcagcaataa agtgataact tttttccgca gcttctgttt
       61 ccgtaatcat tccctgctcc agcaaaacat gggtgattcc attgaaaaac tgatgtaata
      121 gaaatgaaat aaaacctgtt gtttcaactt ttactgggcg acaatgaaac tgcatatcct
      181 gccaaagttt aatatcatct tttaactgtt gagagctcac aatttcatat acaacagcag
      241 aaatattaac taaattaccg ttgttattca gccaaattgt ataacgattc caatctcctt
      301 ttctcattgg ctctatacaa atgtgaaata ggtattggta ggctttatta tttaaattgc
      361 ggttatccca gatgtatttg agtaaatcta aatcgtgttt cataatgttt ctccaaaaga
      421 aaaaagtgga gaaacatctt acctataggg aaaatatttc cccacagggt aaaaaacaat
      481 ctcgataagc ggtcaaattg aaataaaaaa cctacaaatc aggcatgcaa gtaggcaaaa
      541 cgtgcggcat tactacgagc agaagctata gcattactaa taagctcttg tcgataacca
      601 ctcaatgaat ggtctgtcgg cgaatagctt acgcctgtaa tttcatcgct acctagttga
      661 atgccacttt ccttatcagt aacggttgct acaaaatcag catagcaaag tttcccttgt
      721 aggatttcct ctatcattgc ctcaaaatcg cagacatcac cacatagcca atcaacagac
      781 tctgaataaa attcagagga tttacgttta agttctatca gatagttttc cgtgttaaat
      841 ttgaaaatag tttccgtctg atacggtgag gcctcaataa tctgatagcc attcgattct
      901 aaggcacagg aagtatcttt aatatactga cctaaatctt gaataaatcc atcccacagg
      961 gctttgtatt tttccacttt tgatgtaggg aaataccact cttttcgggc atcacgatca
     1021 tagaaaaatg ccaaatcatc tgctgttcta acagaaagct ccatcgtatt acaatgcgaa
     1081 tagcggtacc caaaactatt tcgtatgatt tcaaaatcca cgccatagtg aggctcccaa
     1141 gattcaatgt gattcagcag attagaaaac attttttgtg tgcgtttctg aggatttagc
     1201 ttgttgaaaa gattaattaa cgtctccgta ttaagcctac cgtagaatgc tacgccatcg
     1261 ccttgacaat gagaaagtga atactcaatg tttaagcaat taggaaaacc agctttaaca
     1321 agagcgtgtt taaaataagt gtttaagact gagccagaca ttgcgcttct cctaaaatta
     1381 gagggaaaca cattaccctt ttccggaaat tgtgtttccc gaaagggtga aagattgatt
     1441 accacttatc gaagttaatc gctctgaatt tataaacgga tataggttga tcatagagtt
     1501 ctgtttccga aatccaattg tgttcaacca agatttctct gacaagatac ataaatgtac
     1561 tcatcaaatg acgactatga ctaaacccgt ttccaccaac ataagcaaac cctctcaggg
     1621 ttttgcgaat tcctgcttta acaacaaggt aagtaatatc aacctcgcca taagacgggc
     1681 agacatcaaa gcgagaaaaa acagaaaatg ctttgccaaa cttggtctca cgctctaatt
     1741 tcacactgaa tagatcatgt gcatggcgta aattccaaag tacactgtat aagtgcaaat
     1801 ctgtttcggt atattgaatt tttttacgag ccataatgtt tctcctaaaa aataaaaggg
     1861 aaacattact tccctctggg gataatgcct ccccttgtga ggtataagaa taggatgtga
     1921 atatgaagtt gcctatgctc gactaacaat tagcttgccg ttctgctcgg acacactcag
     1981 aaagcgaaaa ttaagtcctt gccgagacgg catttgttgc ataaatcgca cgcttttgat
     2041 gtccttatct cgattttgcc acgcttctaa agctctatga ctaatttctt gctgtaaggc
     2101 ttgatgctct gatgtccatt cattaagttt agttggtaaa acatctccaa tacaggaaag
     2161 tctaatatga actaacatac atttctccag aataagggga aatgcattcc ccttaaggga
     2221 aacaacattt ccccgatggg tgagttaaat tagattaaag agagctgagt accaaaaacg
     2281 gcagccatac cacatacaaa ggcaataatg gcagccaaca aagtccagaa aaacatctta
     2341 cgcttaatgc gggaaatttc ctgttcatat tcggctgctt gttcgtctaa gcacatttgc
     2401 agttctaagg ctcgcatttc atcttcttca gcaatccgtt gattgatgcc tttggtgact
     2461 tcaacatact tgcttttggc tttatgtttc gctgaaacaa aaatattacc tagcttgttg
     2521 ccaagtttcc aaaaaagaga aggttttacg gttttggatg attcaaacgg gataacatcc
     2581 catcctgcgg tttgttgact catagcgagt tctcctaaaa gttaagtgaa taaaattcct
     2641 caggagatcg caattccaag ggaattgaaa tatccctcgg aaggtaggtg tgtaggtagc
     2701 attcctgcaa atttgcagag ttccctttca gaatgctggg agacattttg ggtaaataac
     2761 tcaactacct ctaaggatag ccgccgcttt tcaagagtta tgcgactttt aagggctgta
     2821 tctacagcgt cttaatgctt aatatcatac gaattcctcc aataaaatca accgtttttt
     2881 tgttcaaaaa atatctcttt tttgaaaaga gtttttccta tcctcaacat aaaaatctcg
     2941 ctaattttct gcttatttgt tcgggaggag agcgtgatga aagtaagtag atttctggga
     3001 tattgcagta tagtaacagg tgtgttttcg ccccttgcac aagcacaacc caatatcatc
     3061 gtctatacca caaagatgta ccctattcaa aattcacaac ttgcttccca gatttattat
     3121 cttgaccaag tagagaattt tgaagagtca atcagtcgaa acctcagtaa atcaccagag
     3181 attgccgaac aagaaatgcg tgcattcttt aattcagcag attggaaagc ccaagagcaa
     3241 caattaaaag acgcttatca aggcgtgatc agtggatggc aaaatggcat taagaaagta
     3301 cctgcggtgt tttttgataa tccacaatct tctccgagtg tgatttatgg tacgactgac
     3361 gtacagaaag cattagatga atggcataaa tgggttaaac aacaaggtga atcaaaatga
     3421 aaaaaacgtt tcttacgata ctgatctctg ccagtttagc tgtcccaaca accttatatc
     3481 ctcaaccagc cagagcctca ttgaataccg cttccattgc tgcatcaagt gcctcaacaa
     3541 gctgtttgga atataaagtt gttggaacct gtttttggtt attttgtacc tactttggat
     3601 gtaaggttcg cacatcaacc aaaattaagc attatttacc tgaaatggtt gtttcatctt
     3661 ataaccacga tgggcaaacg ccatggacag aattacaagc tctcaatcct ggtgtaaaag
     3721 gtggagaata ccgttctcca caacgccaat actctcaact cactttcaaa aatgcggatg
     3781 cgtttggaca cccacaagga gctatcggtc aattattagg aaatacgggg tactattgta
     3841 aaactcaaac tacgccattt atgccttact ttttgagtgg gctggatttt tttgcctggc
     3901 gtgaaaatat gccggaaatg ttttacccgg aagctctgac tccgggaatg cgggaagttg
     3961 gccgtgtagg cgatagctgg ggaaatattt acccacgttc cggttgggta acccaagcac
     4021 acgattataa agcctctgcg accattgtgc agagagtggc agacattgtg agccgaacag
     4081 ctcagccaca catttatttc ccagcagcca aaacaggtgg tgcaggagaa tggcctccag
     4141 gaccggtcga agagggacaa gcaagaacac ataaatggca aatactttac cctcaaatgt
     4201 caaatacctg ctctgttttt cctaatggag ggcaaacaga tacctattct tctcaaattt
     4261 caaatcaagg tgattatgct tgggcattat ggcgacctta tagttgttgc aaacgccgtg
     4321 ggcaaacatt cctttacagt attgattggt cacactaata acattgggag caataatgaa
     4381 atctcacttt aacttcaaaa aatcccactt acggatcacg acagcattat tatcaagtgt
     4441 atcaggacta tcttatgccg attcgattac ggataaactg gaatttagta agtcggattc
     4501 gattatttca gaccgtgttt tttaccaaat cggcggtgga atgggttata tggctccacc
     4561 tagtcgtggt aataccaaag ttgttgattt aggaattggt tggaaagcga atttaacctg
     4621 tggtaatttt gatattaaaa cgaccattaa aaatgattta aacggtctca aagacggctt
     4681 taaagattta atggggaatg tcattgattc agcgaaaggt gcggttgcaa gtatgcctgc
     4741 aatggtattg caacgggcca accctcaact ctacgattta ctcacaaatg gtatgtacca
     4801 aggtaagctg gatttcaacc gtcttaaaac cagttgtgaa gaaatgtcca aagttttagc
     4861 agatcataca ctgggaggaa aatgggggca agcagctgat ttagaaagct ataagcaaat
     4921 tacctcctct caacctgatg caaaacaagc tcaaaaagac ctggaagata aaaaagggaa
     4981 agatgggcga gtttggatag gaggtcagaa aaaaggtggg gaaaaccaag aagctatcaa
     5041 tgttgttggc gatgttgtca aagctgggtt taatttactg aacaaacgta atgccttaga
     5101 taccagtaca atttcaggtt caagctgtga tgggaatctt tgccaaactt gggataatcc
     5161 acaaacagca gccgattttt taactaagat tattggtgaa caatctattt cgacttgtaa
     5221 agaggattgt ggcactaaaa ccagctcaaa agccggtgta ggcttgagtc cggaaattga
     5281 ggaagaacat gttcgtacta ttgtcaaatt agagaatgta ctgaatatgg atgtgcctaa
     5341 ggctgaattg ttagctgatt tgagttcaac tactgtacca gtaacaagag ggcttattga
     5401 gagtcttcga gatgatcctg atgttgaggt attaagccaa cgcttatctg cagaaattgc
     5461 gactagtaag ataatggaaa aaatgctcct ggctcggcga gcagtcttag ctggtatgcg
     5521 tgagccaaat gtcgctcaaa atgcggatgc acaagtagaa ctggaaagag tactcaatat
     5581 tattgaccgt gaaatttccc aagtaaaact agaaatggat atgcaaaaaa tgatcaccgg
     5641 caatacggct tcggtgattt tgcaaagcaa atccgaacga gaagctcttg ctggtgctca
     5701 tagtttatct caagacagtc tggataaacg tgttcaagga cttgaaaacg gtaatgttgc
     5761 attaactgat ttagaggcag gctctaatat taacctctct cgtagaaata ttgcattgag
     5821 tatcccgaat attccgaatg tagctgctct catacctaat ctgccaaatc gaggtggttc
     5881 aggtgtaggt ggaaatttcc catcactcgc acaaatcagc ggttcaacga tggatcaagc
     5941 aacaggatta ctacgtaatt ttgaaggctt tatctctaag gctcaatggg atgtaaatgc
     6001 tcatcgagta ggatatggtt ccgatacaat tacccgagct gatggtacga tcgtaaaagt
     6061 aaccaaagat acggtcatta ccaaagaaga tgcagagcgt gatttggccc gccgaacagc
     6121 tatcttcgca aataatgtga gaaaagagct tggagattca aattggaatg cgttaccgcc
     6181 gaacgctcaa gctgttttag tgtcttatgc atataattat ggttcacttg ctaaaacaaa
     6241 aagcgtactt gatgcagcca gaagatcagc tcaaagtggt gatatgaatg ctttagctac
     6301 tgcaatcaga aatcgccaag ttgataataa tggcatcaat gcacgtcgcc ggaatcagga
     6361 agctgactat attcttggta aaaattaacc tcataaggag ttacatttat gcgtaaatta
     6421 ttaggtatta ctgtacttac actttgtaca acttcagcat ttgcaacgga taaaaatgtg
     6481 gtattttctt gtaccagcac agaaggtaaa cctctcacag ttaaacgggt aggaaatgat
     6541 tacgagtatt cgtatgataa aactatcttc aaaaacccaa tcaaaaaagc ggtgaccaac
     6601 gatggctcaa ttatcgctag aggatcaggt tttaccacgt atgcattaga actgaaaaat
     6661 gatggtttga agtatgtagt tgggtttgtt caaccaaatg gtaactcaaa agagtttatt
     6721 gagcctggag caacgattag ccgtgataat gatcaaccaa gcattggctc tgtagattgt
     6781 gatccttaca aaaaaatcta ttataagttt gatgttcatt taatgaatac cttgtaatgc
     6841 gataattcaa taatatgggg gaatggcttg atttttcatc ccccatactc tattatatag
     6901 atgtggtgct agagccagcc cacgttaaaa aatacaaagc tcggtagatg aactattgtg
     6961 gtgccagagc cggcccacgt taaaaaatac aaagctcggt agatgaggcc ccttaagtcg
     7021 taagatttaa ggggttttgc ttaggatata aaatggatat tacaacacaa aatattgaac
     7081 ttagatactt atctgagcag ttttaccttg atgtcgctcc acatctgaaa gaaattttgc
     7141 ttaaagagac tcgcccatac tgtgttctat tagttagagt taaaaattta gattttgccc
     7201 taccattgcg ttctaattta cctgaaagag aaggagttgg aattaaaacg atacctaatc
     7261 ctcaggaata cggcaaattt aaaggaattg atttctcaaa agccattcta ctgacagata
     7321 aaagctattt aaaaacagat accgttcagc tcaaaaacaa atcagaattg ttgaatatcc
     7381 aaggtaatga gcgtagaatt gttcgtgaat ttaagaaata tgtgagtgaa
//
LOCUS       DANLUA010000127         7360 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLUA010000127 DANLUA010000000
VERSION     DANLUA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..790)
                     /locus_tag="PXB44_001905"
     CDS             complement(<1..790)
                     /locus_tag="PXB44_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5282356.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWA"
     gene            complement(891..1967)
                     /gene="fbaA"
                     /locus_tag="PXB44_001906"
     CDS             complement(891..1967)
                     /gene="fbaA"
                     /locus_tag="PXB44_001906"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL5282357.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(2064..3239)
                     /locus_tag="PXB44_001907"
     CDS             complement(2064..3239)
                     /locus_tag="PXB44_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL5282358.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLATELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(3494..4852)
                     /locus_tag="PXB44_001908"
     CDS             complement(3494..4852)
                     /locus_tag="PXB44_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5282359.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(4922..5749)
                     /locus_tag="PXB44_001909"
     CDS             complement(4922..5749)
                     /locus_tag="PXB44_001909"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL5282360.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(5763..6851)
                     /gene="aroB"
                     /locus_tag="PXB44_001910"
     CDS             complement(5763..6851)
                     /gene="aroB"
                     /locus_tag="PXB44_001910"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL5282361.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(6875..>7360)
                     /gene="aroK"
                     /locus_tag="PXB44_001911"
     CDS             complement(6875..>7360)
                     /gene="aroK"
                     /locus_tag="PXB44_001911"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL5282362.1"
                     /translation="MGAGKSTIGRQLAQTLGMEFMDSDSVIEERAGADIDWIFDVEGE
                     EGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIYLETTVDKQFERT
                     QRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKVVANQIIDMIDNL
                     Q"
BASE COUNT         2272 a         1648 c         1280 g         2160 t
ORIGIN      
        1 ttgcccaagc ataggaacta aggtgctttc caaaatgact ttcaattaaa cgtttcgtaa
       61 tttgatgttg cggattcagt aaaacctctt tcgtcaagcc atactctact acttcgccac
      121 tttgcatcac cattatttta tcggcaatat gtttgatcag acctaaattt tgccccacat
      181 aaatataagc aatcccttgt cgctgctgga cacttaacat taaattaatt aattgtgttt
      241 tgactgaaaa atccagtgaa ttaatcgtat catccgcaat aataatttcc ggttctaaaa
      301 ttaaagcacg agcaaacgca atacgctgct tttgcccact ggaagcctct gaaataggaa
      361 cgagcgcttg ctcaggatac attcctacca attttagcgt attaaaaata cgctcatttc
      421 tttcctcttc atttaaatct gtcgccaagc ggagcggagc atctaaaatt tgtcctatat
      481 tataattagg atcaaaggca tcgttagggt cctgaaacat catacgaata tgcttagctc
      541 ggtatttata atcgccaaat gaaagcggtg ttcctctaaa aatcagttct ccagatgttg
      601 gcccaaccat accagcaacc attttagcca aggtagattt cccagctccg ttttcaccaa
      661 taatcgctaa agtttccttg cgatttaggg taaaagaaac ttcttttacc gcataaaaat
      721 cttgtttatg aaataggcta ctacgttcaa caaaacgctt attcagatga ttaacttcaa
      781 gtaatgccat aataaattgg tgcttggaaa taaagtgata gtttaccgca aaaaataaat
      841 aaaaaaagac cgcttataca agcggcctga tttatggaat tttttgcaaa ttataaaacg
      901 ttcacacagt ttaagtcttc gaaagattgc tctaagcgtt tagacataga ctcttccatt
      961 ttacgtaacc aaacacgtgg atcgtagtat tttttgtttg gtgcatcagg gcctgtcggg
     1021 ttacctaatt gaccttgtaa atatgcttcg tttgctttgt agaattgaag aataccgtcc
     1081 cacgcagccc attgggtgtc agtgtcaatg ttcattttga ttgcaccgta gctgattgct
     1141 tcacgaatct cttcacgtga agaaccagaa ccgccgtgga aaacgaagtc taatgacttt
     1201 gccggtaaac cacgctcttt agatacatac tcttgtgaag cacctaagat tgatggtttt
     1261 aattttacat tacccggctt gtaaacaccg tgtacgttac caaatgctgc cgcaatcgtg
     1321 aaacgtgggc tgattgggct taattggtcg taaacatata aaacttcaga aggttgggtg
     1381 taaagtttag attcatctac atcagaatta tctacgccat cttcttcacc accggttacg
     1441 ccgatttcga tttcaagggt cataccgatt ttgtccatac gggctaaata ttcacggcag
     1501 atttccatat tttcttcgat tggctcttca gataaatcga tcatatgtga agagaataac
     1561 ggacgaccgg tttctgcgaa gtgtttttcg cctgcttcaa gtaagccatc gatccaaggt
     1621 aataagtttt tcgcagcgtg gtcggtgtga agaatcacag gcacaccata ttcttctgct
     1681 aattgatgaa cgtgttttgc acccgcaatc gcccctaata catctgcacg agcgcctgaa
     1741 gccggcttaa tgcctttacc cgcatagaac tgcgcaccac catttgagaa ttgcacgatt
     1801 accggtgatt ttacacgagc ggctgtttct aatacggcat taactgaatc tgaacccacg
     1861 cagtttactg ccggaatagc gaagttgtgt tctttagcat aagcgaaaac tttttgtacg
     1921 tcgtcgcctg ttactacacc cggttttacg atgtttaata atgacatttt tttcttcctt
     1981 tgtatgaaat gtgcaaaagc acattgttaa aatatacggc caaatcttgc aaaattttca
     2041 caaaatttga ccgcttgtaa ctgttagccg ttagctcgtt tttctaagat ttccactgcc
     2101 ggtaatactt taccttccac gaattctaag aacgcaccgc caccggttga aatgtaagaa
     2161 attttgtctt tgataccgaa taaatcaatc gcagctaagg tatcgccacc accggcaatt
     2221 gagaatgcac cgtttgccgt tgcttctgca atcgcatttg aaatcacttc tgttccttta
     2281 cggaagttag ggaactcaaa cacgccgacc ggaccattcc aaaggatggt ttttgaatta
     2341 cggataattt cagctaattg ctctgacgat ttttcaccaa tatcgaaaat tgattcatca
     2401 gctcgtactt cactcaccgc tttttccgtt gccggtgcag tttcagagaa ttcagtacct
     2461 acacgcacat caaccggaac aggaatattg gttacttgtg ctaaacgttt tgcttcaggg
     2521 attaaatctt cttcgtataa tgatttaccc acagcgtgac cttctgcggc aataaaggta
     2581 ttggcaatac caccgcccac gattaattgg tcggcaattt ttgaaagcga atcaagcaca
     2641 gttaatttag tagatacttt agaaccaccc acaatcgcta acattggacg ttgtggctct
     2701 tttaatgctt tgcctaatgc atctaattca gtggctaata acggacctgc acacgcaaca
     2761 ggggcatatt ctgccacacc gtaagttgaa ccttccgcac ggtgagctgt accgaatgcg
     2821 tccattacaa acacatcaca aagtgatgca tatttttttg ctaactcagg gtcgtttttc
     2881 ttctcacctt tgttaatacg cacgttttca agcaccacga tttcattctc gtttacttca
     2941 acaccgtcta agtaatcacg cactaaacga acaggaacac ctaaatctga tgcatttaag
     3001 taatcaacta caggttgtaa agagttttcc tcttcaaata cgccttctgt cggacgacct
     3061 aaatgcgaag ttaccattac tttagcacct ttttgtaacg ctaattttaa ggttggaatg
     3121 gtagcaacaa tacgggcatc tgatgttact ttgccatctt ttaccggtac gttaaggtca
     3181 gcacgaataa ataaacgttt acccgctaaa tcaaggtcag tcattttaat aacagacata
     3241 gtttgttcct ctttagatta aaatttaaaa gtgataacgg tcggattata tcagtttcta
     3301 atcattttgg gctaacctag atcaaataaa tgattaattt tttatcattt tagaatgcta
     3361 ttatcctgac tgttcttatt cccccccaat atgcttggtt aattaagcaa gaattgcaaa
     3421 ttccccacaa aatttaaccg cttgtcacta aaaataaagc tgtttgaaaa tccattcaaa
     3481 cagcttatag aatttagagt gaattaaaga aaatcaccaa gataatcacc ggaatcacaa
     3541 agcgaacata gttaaaccaa atagtggtaa acattttacc cgcacctaat tcctccttcg
     3601 cctcttcttt tagaacaaag ccaacgaaaa tcgcgctacc taaggcggtt aaaataaaca
     3661 gaatattgcc actaatataa tcgaaggcat caaaaatgct cttgcccata acctgcacat
     3721 cttgcaatat attgtcactt aatgctgatg gaatattacc caacagaaaa atagtagcca
     3781 aagtaataaa aatagctttt tgacggctaa tctttgcctt ctcttgcaat gcagtgataa
     3841 taacttcata aatagttaac gaggttgtta atgccgccac cactaataga ctaaaaaaga
     3901 taaccgcaaa aactgttccc ccccacatat ttgagaacac aatcggcaaa ctttggaata
     3961 ctaaagtcgg acctgaattt ggagcaacgc caaagctaaa taatgacgga aaaatcataa
     4021 aaccggcaag taccgcaatt aaggtgttca tcaccccagt aattgttgct gtttttacta
     4081 aattctcgct cttatcaagg tagcttgaaa gggttatcaa cacaccaaaa ccaaggctta
     4141 atgcaaaaaa tacttgaccc agcacaaaca caaacaattt tggtgtgatt tttgaaaaat
     4201 cgggcatcag ataaaaggca atgccttcag cagcccccgg taaggtaata tttcgaacca
     4261 ccatcacaat caggaaaata aacagaatcg gcatcaaata tttcactgaa cgctcaatac
     4321 cgtccacaat gccttttacc aaaataaaat aattcaccaa tacaaaaagt gccgtataag
     4381 caataattgt cattgggcta ttaaaaatac tttgttggaa gaattgttgg gtcgtgccaa
     4441 cggtaacagg ttgagacaga tctagtgtcc ccgtgagtaa gctaccgata taatttaata
     4501 cccaaccacc aagtaccata tagtaggcaa gaataccaaa tgctccaagc aagcccatat
     4561 agccaagaat tttccaacct gaggcaattt ttttaccatt agcaaaaccg gcaaaagcat
     4621 caatagcatt cactctcatt cggcgaccaa ttacattctc cactaatatc atagggatac
     4681 caattacgat catcgcaata caaaataaaa acacataagc accgcctccg tgttccccca
     4741 caagataagg gaaacgccaa gttgcaccaa aaccgactgt tgcaccagca acggtcataa
     4801 tatacgccaa tcggttagac caagattggc gttcttgttg ttgatttgcc atttaatact
     4861 ccatcggcta acaataccgc ttcaaccaac aagcggtcag atttttagaa aatattacaa
     4921 attaaatgaa gagtgcgaat aactcgccaa ctttaaccct tgagcccacc ttctgcccta
     4981 ttgaacgctg tacctgaatt tgggtaatat cggcagattg ataaatccta cgggtaaaat
     5041 cagtatcgtg atttgaaatt aatacgggaa cgcctaatcc tttcatttct aaagccagtg
     5101 ttgccaaacg cccttgttgc tctaggctaa aaccaccgcc agcatattgc gtaaaattcg
     5161 tttcttgtgc taaaggagcg taaggtgggt cgcagtaaat cacataatcc gttaattgat
     5221 tttttgccaa ctcaaagacc tgttcaaaat ccgcacacac aaaagttgcc ttttgtgctt
     5281 tttcagcaaa aaaacgtagt tctttttctg gaaaataatg ggttttataa cgcccaaagg
     5341 gaacattgta cccttttttc tggttataac ggcaaagtcc gttatagcca aatcgattta
     5401 aatacaaaaa aatcaccgag cgacgaaaaa tatcttttga gcgattaaac tccgcacggc
     5461 gtgctttata aaaggcttct gtattcgctt gtgggtgaag aaacagttgc tttgtttgcg
     5521 caatatattc ctcaacatct gctttcacca cgttgaaaag attaatcaag tcaggattaa
     5581 tatcagcaag aatataacgt tcaaaatctg tattcagaaa gaccgaacct gcaccaacaa
     5641 aaggctcgac taaacaagcc ttttttggca aatgggtttg aatatctgga attaagcgat
     5701 atttaccgcc agcccatttt aaaaaggctc gatgcttagg gtgattcatt tcattttaaa
     5761 cattaacgtt gggtaaaacg ctcaatagag gctaatactt gagattctgt ggcttgcgat
     5821 gaaacgtaag cttcaccaag tgatttcaat aaaattaggc gtaattgacc tgccagtact
     5881 tttttatctc gccacatata aggtagatat tctgccggtt ccattccatc aggtgaaatt
     5941 gttggcaaat tggctcgggc caataatttt tccaagcgag caacctcttg aggacttaaa
     6001 tcccccagaa tacgagataa ttccgcagcc tctagcatac caaccgaaac agcctctccg
     6061 tgtaaccata caccatagcc catacgtgct tcaatggcgt gaccaaaggt atgtcctaag
     6121 tttagtaatg cacgatctcc tttttccgtt tcatctcgag caaccacgtc agcttttagc
     6181 tggcaacaac gctgaataca atattccagc tcatcttgct tgagactgac caaatcatca
     6241 atatgctttt caagccattc aaaaaaagta acatcaaaga ttgcaccgta tttgatgact
     6301 tccgccaaac cagcactgac ttctcttttg gataaggtat ggagcgtatt ggtatcaaca
     6361 atcacagaaa caggctgata aaaagcccca atcatatttt taccgagtgg gtggtttact
     6421 gccgttttcc cgcctactga agaatcaact tgagccagta aagtggtcgg aatttgtata
     6481 aaacgaatac cacgttggta agaggcagct gcatagcctg caacatcacc gattacacca
     6541 ccgcctaaag caattaaagt ggtatcgcga ttatgatttt tttctaataa tgcggtaaaa
     6601 atcaaattta atgattctag cgttttatat tcttcaccgt cgggaatcaa taccgaatca
     6661 acgtggcaac ccagctcctt taacgcattt gttaccacac taagataatg tgtcgccacc
     6721 gttggatttg agacaatcat caccttatct ccagatttta atgggctata actagcaggc
     6781 tgatttaata accctgcacc aattataatc gggtaacgac gctcttttaa ttccacattg
     6841 acttgtaaca tatcttagct cctttcttac ctggctactg taaattatca atcatatcaa
     6901 taatttgatt tgccactact ttagcgctct gctcgtccgt atgaatagta atatctgcaa
     6961 tttcttcata caatgggtta cggattttcg ctaactcttc caacgtttta cgggggtctt
     7021 ctgtttgcaa taaagggcgt tttttatctc gctgagtacg ttcaaactgt ttatccactg
     7081 tcgtttctaa ataaataacg ataccacgag ccgataacac attacggtta tctttagata
     7141 aaatcgaacc accgccagtt gaaagcacga taccataatt ttgcgtcagt tcatttagaa
     7201 tacgttcttc acgcttacgg aaaccctctt caccttcaac atcgaagatc caatcaatat
     7261 ccgcacctgc tcgttcttcg atcacactgt cggaatccat aaattccata cctaatgttt
     7321 gagcaagctg acgaccaatc gtacttttac ctgctcccat
//
LOCUS       DANLUA010000128         7349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLUA010000128 DANLUA010000000
VERSION     DANLUA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(11..1786)
                     /locus_tag="PXB44_001912"
     CDS             complement(11..1786)
                     /locus_tag="PXB44_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5282363.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPMRKPEGFKTSDTVAGFNIGYNF"
     gene            1941..3026
                     /gene="queA"
                     /locus_tag="PXB44_001913"
     CDS             1941..3026
                     /gene="queA"
                     /locus_tag="PXB44_001913"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL5282364.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(3053..3591)
                     /locus_tag="PXB44_001914"
                     /pseudo
     CDS             complement(3053..3591)
                     /locus_tag="PXB44_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3604..5886)
                     /locus_tag="PXB44_001915"
     CDS             complement(3604..5886)
                     /locus_tag="PXB44_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL5282365.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(6028..7242)
                     /gene="sstT"
                     /locus_tag="PXB44_001916"
     CDS             complement(6028..7242)
                     /gene="sstT"
                     /locus_tag="PXB44_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL5282366.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMF
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
BASE COUNT         2258 a         1577 c         1440 g         2074 t
ORIGIN      
        1 caatcattca ttaaaaattg taaccaatat taaaaccggc aacggtgtct gaggttttaa
       61 acccttccgg ttttcgcatc ggtctaccgg caaacacgtc ataactaaat cctttaactc
      121 caccccgaaa cccaatggct gttcccatta aatgatgtcc gagctgattt tccgttgccc
      181 aaccggacac tcgaccacca tctaccgccc aataaaaagc gtggttcgga ttaaattgcc
      241 agctcaactc attacgccag ttccagcccc gctcaccgga aagagtcagt tcaccgtcaa
      301 atccacgtat ggtgtaacgc ccgccaatcg aaaaacggtc ttgcggaacc agaggggttt
      361 tattccattg ggcatttagg ctactttgcc actgccatgg ctgcttgccc caaaaaaacg
      421 gtttcgtcag gcttgcactt gccgtgatga ttttagggcg agaagtccct tcgccataat
      481 cttcctcggg agcagataat gcccctcgtg ctgccgtacc tcgtttgaaa ttagccgaaa
      541 gctcaagcgt agcggtcggg aaatattctt tatgcgaaaa acccgcttcc caacctgcca
      601 ttcggcgttt ttgtacttca atttctgagc cgtcaatata gttttgcgac tggcgtggcc
      661 aaagtgaacc ggacaagctc gttttacgtt tggcatcacg ataaagcaaa taagaaaagg
      721 ttaatttatc ggtatcactt tcaccggcgt agagatagct cttgtcaaag gcaccaaata
      781 cttcttgatg ataacgatga tgagtgtgtg aaaatgctaa ctgccaatag ccaaacggca
      841 cggaatagta aagcgtgaga tttttactgg cacgtctgcc gttatcatca cctttttgtt
      901 tcatcgaatg ggtaaaagag gtatagaaca ggtcatttgc cgaaaaaata ttatctatag
      961 acagagtggc ggaggtttgt aatttgcctg tggatttcga gccggcatcg tccagcccaa
     1021 gatttaagcg aaatggaaac gcttgcgaat agctgatttt taaatcactg tctcccacct
     1081 ccgctttttc gcttgagctg ggtaaaatct caatatttgc ctccgccgtc ggcacccgtt
     1141 ttagattttc cagagactgc tcaatatctc gcacattcag aatatcgcct tcggcaaaag
     1201 taaaaccggt taaggcgtgc agcttggtaa aacgcggtac tttgccgcta tctaccacta
     1261 aagtattgcg tattttgccc ggaatcacgg ttaaaactaa ttttccgcca cgtaaatctt
     1321 gctccgcagt aaccactcgt gtagtgacat aacctttgcc aataatttca ttttggattt
     1381 gcttcatcaa cacagctaac ccttcaccac caaagcagtg aggtaatgtc aaatgaaatg
     1441 attttactgc tttttcaaac gcccattgga attggctggt agtcagagaa ttgtcaggag
     1501 aatagtcaat gagagtgatt tgatgaatgg ggtaacaagg caattcgtga ttggaaagag
     1561 taagtgtatc attgatttgt gtctcgagcc gtacatcagc tttcggctgg aatttttctg
     1621 cttgattttg ttggtattgc tgttggcgat gttgctgggc agcatcaatt tgttgttgag
     1681 ttgcatcaat gcgtgcttgt tgtacaaggc ttgaagaatt tgcttcagca acaaaagaag
     1741 cacttaacaa taccgtgctt aaaatcagat tttgtggggt tttcataaga tcaaataagt
     1801 aagacattca acgtttttag tctataaaaa tttatgttgt tttgcaaact ccgttttatg
     1861 gagctttata catatctgac gactttggtt ttttatgcta ttatctccgc catttttata
     1921 ctatagagaa tagaataacc gtgttagttt cagattttca tttcaactta cccgatgagc
     1981 tgattgctcg ctacccgact gccgaacgct ctgccagccg tttattacac ttaaatggcg
     2041 aaacaggcga atttgccgat caacaatttg tggatttata cgatcatatc aacgaaggcg
     2101 atctattgat cttcaataac actcgagtga tcccggctcg cctttacggt cgaaaaccta
     2161 gtggcggcaa agtagaggca ttagtggaac gggtgctaga tgaacaccgc tgcttggctc
     2221 acgttaaagc ctcaaaagca ccgaaagaag gggctgaatt gatctttggg gaagataagc
     2281 taggtgaagg caaaggcttt acggcgacaa tggtagctcg ccacgatact ctgtttgaat
     2341 tggtgtttaa cgaagagcaa ccgttattcg aattgctaca gcaagctggt catatgcctc
     2401 ttccacctta tatcgaccgc cccgatgaag atgctgacca agagcgttat caaaccgttt
     2461 atagcaaagt gttaggtgca gttgccgccc ctaccgccgg tttgcatttt gacaatgcaa
     2521 tgcttgaaaa actaaaagca aaaggggtgg atactgcctt cgtaacattg cacgttggtg
     2581 ccggcacttt ccagccggta cgggtagaaa ccattgaaga tcataaaatg cacgccgaat
     2641 atgccgaagt gagccaagaa gtagtcgata aaattctcgc aacgaaagcc aaaggaaaaa
     2701 gagtgattgc aattggtacg acatcagtac gttcggttga aagtgccgcc caagcggctg
     2761 aaaaagatgg gaaattgatt gctccgttct tttctgacac cgctattttc ctttacccgg
     2821 gaaaaacctt taaagtcatt gatgcgttag tcaccaattt ccatttaccg gaatccacgc
     2881 taattatgtt agtctcagca tttgccggct atagaaatac gatgaatgcc tacgcccacg
     2941 cagtagaaac taaataccgt tttttcagct atggcgatgc gatgtttatc aataaaaacc
     3001 caaatgcgtt gagtgatgtt ccttaattta tgagggttat aagggaataa cattatgaac
     3061 aaaaacaatc ttccaaccat tttaagggat tgttttcaat atattcaata atttggaagt
     3121 acgatgtttc attacgaata atgtgttcat aataattcct ttgccaaatt tcagaaatat
     3181 cggtattttg acgaacccat ttagttacgc caattttaaa accacgcaca atagagccga
     3241 ttgttcgtga tgttccccgt aagggcgaaa catttttcgc ccttatacat ggggcaaatg
     3301 atgatttgcc cctacagttt cgacgatttc aataatgcca tgtaaatgat taggcataat
     3361 cacaaagcta tgcaagctta catttggaaa atgatcgggt atatcttccc aacattgacg
     3421 tacaatattc cctatcttat taagcctcat ttcatcatca acaatctcac cgagaaaatg
     3481 gtgtttattt ttacaacaaa tagtaatgaa atagaatcct tgtgatcggt aatcataatg
     3541 ctttagacga attgaacgcc tcctatgaat ctccggctga tatttctgca tttttcttat
     3601 tccttaataa aactgcttat accaagggga aaatgccccc cttgcctgct caatttcaat
     3661 agaacttggg taaaatttta ctcttatcat tcccgtttct acggtattta atacattaat
     3721 atgtcttctg tttaatctct caataatctg tttattcggt aatttccatg ggttccatct
     3781 tccagcagaa atcactgcaa atgctggctg ggtaatcgat aataaagttt cgccagtgct
     3841 gcttttgcta ccgtgatgtc caacctgtag aaaatcaatt ttgccgagct gatgggcaaa
     3901 ttggcgttct tgttccacgc cagaatcccc tgtcagtaat atcttcaagc ggtcgttttt
     3961 ggctaaaata acgcaagagt cttggttttt agctcgttct gcaatgtgtg acgggtaaat
     4021 tgccaataat tgccattcgc caaattgcca cgatgttccg gcaatacaag gctcgggatt
     4081 tatattgtga tacgtcacct tgcttgaact aattaacctt gcttttgggt atgcttgcaa
     4141 taactccact accccacctg aatgatcatt atcatcgtga cttagaaaaa tcgcctctac
     4201 ctcaatgcca tttcgcttta aatacggcaa gatttccatt tttgccattg aattgcgact
     4261 accatcattt gcttgccaac tggagccggt atcgtaaatt accgctttat tttgttgata
     4321 aatgaacgcc attgccagcc cttgcccaac atcaaacata atccattctg tttgggtttg
     4381 attaaagagc ttagggagat aaaaactcag attgaacaat agggataccg ctactgcact
     4441 taaccacttt ttataagata gccgatataa tttgcaatac agcaatacta aaaccaataa
     4501 attcagtgaa agtaactgcc actgttgcca atggcttaat gcaaaccacg cattagaaaa
     4561 tggatctaac agccataggc taaattgagc aagatagtcc gccaacagcc acgttgaaaa
     4621 cagattgtcg gtcaagagcg taaataaaat aagcggtaca agcaaaaagc tatacaacgg
     4681 cacaataatc aggttggcaa gcagtgcaaa agcagagatg ccctcaaaaa agtagagttg
     4741 aaccggtgag aaaatcagcc aaatgccgat ttgtaagtgc aaaagcgata accacaagcg
     4801 gttaaatttt gtgatttttt tacgatattc atcgtttaaa aatcggctaa gaggaaaata
     4861 ttgataccat aaaattaaac ttgctaccgc taaaattgag agccaaaaac tatcagaaag
     4921 caggctcaat gggtcaatta aaatcagtaa cgccactatt ctcccccaat attgccaagg
     4981 ggtatagtgt cgccgcagaa cttggcaaag caagacaaac gcaatcgcca gcaacgctct
     5041 ggcggtcggc acggcaaaac cggctaaata gctgtaacca aatgcggtga tgaagctaac
     5101 tattcttgca aaataaagtg aaaatccgac cgcttgtaat gcggtaagct cgcttttcaa
     5161 gcaaagccat tgcacgcctt tggcaaagta aacaccaaac aacattgcca gcccgatatg
     5221 caagcctgaa atggcaatta gatgggcagt agcggtttgt tggaattgcc cccagtgttc
     5281 tgttttcagc cacgcccttt caccaaaagc cagagccaat aaaaagcctt gcgtaggcaa
     5341 agcgtccgtt tcagctttca ctttattcag ccaatttgtt cgcaagctat aatcagtttt
     5401 ggctaatgtt tccaccttgc gaaccgttgc tgtcatattg atatgattgg caaaatacca
     5461 acgctgtcgg tcaaaattac cgatattgct acgagctgaa ataggacgaa gagccagttc
     5521 ggcttgatag aattgattaa gtaacaacgg ctgtttcgat tgccagttca agtagatctt
     5581 ctcgcctgta tttagtgtcg caatcgctgt ttgataatcg gtttgtttta agattttact
     5641 gatttcaaat tgaacaggcg ttttactagc agtcgttttt tccgcatttt ttgcaacttg
     5701 aatgatctga atatagccaa tacaaaccaa taagccgagt acagccgtta attttgagcg
     5761 tagataaaca ctcaatatca gcactaaggt tcctattact atacccacaa atagaaacgc
     5821 tttaggcaac cataaaatag gcagatgaaa tagaatcagg gcaatacaaa aacggtctaa
     5881 agtcataatt cggcttactt tttaaagctg agtatgccgt ttgtgcttaa tattttcaga
     5941 ataaattttc agattttcga cacagttcgc aaaaatactt gaaaaaataa aggcagactt
     6001 tttagcctgc cttataaaga agagatatta tgctgatttt tgatcttcag cgtaacttac
     6061 tgctgcagtg aataatacgt ctgttgatga atttaatgcg gtttcaaccg agtcttgaat
     6121 cacaccaatc acaaaaccca ccccaattac ttgagcagca atatcgtttg gaatattaaa
     6181 taaactacag gctaatggaa tcaataataa tgaaccgcct gccacacctg atgcgccaca
     6241 agcacagatt gatgcaacta cgcttaatag tagtgctgtt gcgaaatcag gttgaatacc
     6301 ttgtgtataa gctgccgcta gggttaatac ggttacagta atcgctgcac ctgccatatt
     6361 aatacttgca cctaatggaa tagctacaga agcgatctca tcacgaacgc ccaaacgctt
     6421 ggctaaattc atattcaccg gaatgttggc tgctgaactg cgggtaaaga atgccgttac
     6481 accactctct cgtaaacagg taaatgtaag tgggtatggg ttgcggcgaa ttttccaata
     6541 cactaataat ggatttagta ctaatgccac aaataacatt gcgccaacta agactactaa
     6601 taaacgtgcg taatcttcaa acgcatcaat accattgatg gcaacagttt cagctactaa
     6661 accaaatacc ccaataggcg caaatgcaat cacaactttc acaacaaacg aaactgcgtt
     6721 agctaaatca tttaagaatg cttttgaact tgccgaacca tggcggaatg caatccctaa
     6781 accgatagac cacgcaagaa taccgataaa gtttgcatta cttaatgcga ctaatggatt
     6841 atcaactaag ttaaaaataa cggtttttag aatttcacca atgccttgtg gcggggttaa
     6901 accgtctggg cttgcagtaa gctctaatgt agttgggaac ataaagctta aaacaacagc
     6961 cgttaatgcc gcaaaaaatg tacctaatac ataaagcacc agaatcggct ttaatttgct
     7021 atctgaacca acttctttat ttgcaatcgc agccaaaact aatacaaata ctaaaatagg
     7081 cgcaatggca cgtagtgatt tcacaaaaaa ttgacctaat acaccaacgc tggtagccca
     7141 ttctttatta ataaatgcca gcaatacccc tagcactaaa ccaatggcga tacgtaatac
     7201 taaattaccg ccaagaattt ttgatgataa tgattgactc ataacccttc acttctgtaa
     7261 gacaacaaaa aataaggata aataaaattt atccgcctga aaaaataggt ttataagatt
     7321 agcggaaaat aaacaaatat ggaataaac
//
LOCUS       DANLUA010000129         7291 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLUA010000129 DANLUA010000000
VERSION     DANLUA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2435
                     /gene="thrA"
                     /locus_tag="PXB44_001917"
     CDS             <1..2435
                     /gene="thrA"
                     /locus_tag="PXB44_001917"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL5282367.1"
                     /translation="TSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKITNHLVAIVD
                     KAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQQVAAEAAQ
                     TKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSYLESSVDIA
                     ESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACLKADVCEIW
                     TDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPLNIPCLIKN
                     TGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAARVFSTMSKA
                     GISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDIIKDLSIVS
                     VVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIEAVKATHKS
                     LFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDKMLLNENGL
                     NLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYARALKEGFHV
                     VTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLAAGDEVEKF
                     EGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARKLLILVRET
                     GLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEGKVLRYVGS
                     ITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGNSVTAAGIF
                     ADILRTLQGGVN"
     gene            2435..3382
                     /gene="thrB"
                     /locus_tag="PXB44_001918"
     CDS             2435..3382
                     /gene="thrB"
                     /locus_tag="PXB44_001918"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL5282368.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3497..4873)
                     /gene="cysS"
                     /locus_tag="PXB44_001919"
     CDS             complement(3497..4873)
                     /gene="cysS"
                     /locus_tag="PXB44_001919"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL5282369.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            5029..5538
                     /locus_tag="PXB44_001920"
     CDS             5029..5538
                     /locus_tag="PXB44_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5282370.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(5630..6559)
                     /locus_tag="PXB44_001921"
     CDS             complement(5630..6559)
                     /locus_tag="PXB44_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5282371.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(6562..7281)
                     /gene="fdhD"
                     /locus_tag="PXB44_001922"
     CDS             complement(6562..7281)
                     /gene="fdhD"
                     /locus_tag="PXB44_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL5282372.1"
                     /translation="MPVALVYNGISHTVMMATPANLEDFALGFSLAEGILNAPSELYG
                     LDVIEQENGVEIQLEISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKN
                     NRLQNVNPLILEDCLLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDK
                     LIGWYAKADFPIGFVFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTL
                     LAFARKGKATIYAGEERLALN"
BASE COUNT         2133 a         1574 c         1556 g         2028 t
ORIGIN      
        1 gcacttctct tgccaaccct gaacgcttta tgcaagcggc agagattatc gagaaagctc
       61 atttggcaga tcaagctgcc ggcgtattat ccgccccagc caaaattacc aatcatcttg
      121 tcgctattgt agataaagcg attgccggtg aatcatttca atctaacctt tctgaagcta
      181 ccgagatttt ccacaacatc attaacggct tatatgctac aaataatcac tttgctaaag
      241 aagcactgtt agccttagtg gaagcggaat taaaccaaat tcagcaagtg gctgcagaag
      301 cagctcaaac taaatcgttg tcagataaaa ttgctgccac tattcactgc cgtggtgaaa
      361 aactctctat tgcgatgatg caagcgtggt tcgaggcaaa aggctatgaa gtcactcgta
      421 tcgaccctgt tgaaaaatta ttggctcacg gcagctattt ggaatcttcg gtcgatattg
      481 ccgaatccac caagcgagtt gatgcaggtt caattccaaa gaaaaatatc gtgctaatgg
      541 ctggttttac cgcaggtaat gagcaaggcg aactggtgtt attagggcgt aacggttcag
      601 attattctgc cgcttgtttg gcagcgtgtt taaaagccga tgtttgtgaa atctggacgg
      661 acgtagatgg cgtttacacc tgcgaccctc gtttggtgcc tgatgcagta tgcttagaaa
      721 caatgagcta tcaagaggca atggagcttt cctactttgg ggcgaaagtg atccaccctc
      781 gcaccatcgg gccattagtg ccgctcaata ttccttgttt aatcaaaaat accggcaatc
      841 cggacggaaa aggcacatta attgatggca atgtggcaaa cgataatctc aaagtaaaag
      901 ggattaccaa ccttgataac gtggcgatgt tcaacgtttc aggttcagga atgcagggaa
      961 tggtgggcat ggctgcccgt gtgttctcga cgatgtcaaa agccggtatt tcggtgattt
     1021 taattaccca atcatcatca gaatatagca tcagcttctg cgtgccggca aaatcggcag
     1081 aaaaagcgtt agcggctcta aatactgaat ttgcacaaga gttatctgaa aacttacttg
     1141 acccgattga tattattaaa gatttatcca ttgtttcggt agtgggtgat ggtatgcgta
     1201 ccgccaaagg aattgcggct cgcttcttct ctgccctagc ccaagccaac atcagtattg
     1261 tggcaattgc acagggttca tctgagcgtt ccatttctgc tgtcgtgccg ttaaacaaag
     1321 cgattgaagc agtaaaagca actcacaaat cgctatttaa cagtaaacgt gtggtggata
     1381 tgtttttagt gggtgtaggc ggtgtgggta gcgaactcat cgagcaagtc aaacaacaaa
     1441 aggagttttt ggcaaagaag gatattgaga tccgtgtgtg tgctttagct aattcagata
     1501 aaatgttact caacgaaaac ggcttaaatt tagacaactg gaaagaagat ttagaaaaag
     1561 caattcagcc gtcagacttt gatgtattgc tttctttcat caaattccac cacgtggtaa
     1621 acccagtatt tgtagattgc acttcggcac aatcggtggc gaacctgtac gcacgagcct
     1681 taaaagaggg cttccacgtg gtaacaccaa acaaaaaagc caatacatca ggttacgatt
     1741 actacttaga aatgcgtgaa aacgcccgcc aaagccagca caaattcctc tacgaaacta
     1801 atgtaggggc aggtttaccg gtaattgaaa acttacaaaa cctattagct gccggtgacg
     1861 aagtagagaa atttgaaggg attttatccg gctcgctctc tttcattttc ggcaaattag
     1921 aagaaggttt aaccctttcg caagcaactt taatcgccaa agaaaaaggc tttaccgagc
     1981 cagatccacg agatgattta tccggtgcag atgttgctcg taaactctta attcttgttc
     2041 gtgaaaccgg cttaccgctt gaatttgaag atattgaaat tgaaggcgta ttgccaaaag
     2101 gcttctcgga aggaatgagt cgtgatgagt ttttacaagc tctaccgtct ttagacgaag
     2161 aattcagcaa gcgtgtagaa gctgccaagg ctgaaggcaa agtgttgcgt tatgtgggct
     2221 cgatcacagg tgataaatgc cgagttgcca ttgaagccgt ggacgaaaac cacccacttt
     2281 acaaagttaa agacggcgaa aatgcccttg cttttctcac acgttactac aaccctattc
     2341 cattattatt gcgtgggtat ggtgcgggca atagcgtaac cgctgccggt atctttgcag
     2401 atattctgcg tactttacaa ggaggagtga actaatggcg ttacgaattt atgccccagc
     2461 ttcaagtgca aatattagtg tgggttttga ctcattaggc gctgcaatct ccccgattga
     2521 cggctctttg ctcggcgatg tagttcaaat tgaagagagt gaacaacaat ttgagctaga
     2581 aagtgcaggt tattttgtac gtaaattgcc caaagaacca caaaaaaaca ttgtctatca
     2641 agcctatgtg ctatttagcg aacgcttgaa attacgtaac ggcagtgtaa aaaatttacg
     2701 cttaacactt gaaaaaaata tgccgattgg ctcagggcta ggctcaagtg cctgctcgat
     2761 tgttgcagca ttagtggcac tcaataaatt ccacgatgaa ccgttctcca aaatggaatt
     2821 actggaaatg atgggcgaat tggaaggcag aatttcaggc tcaatccact acgacaacgt
     2881 tgccccttgc tatttaggtg gcctgcaatt aatgacacaa tcgctcggca atatttgcca
     2941 acctctgcca ttctttgatg aatggtattg ggtgcttgcc tacccgggcg tggaagtatc
     3001 aaccgccgaa gcccgtgcta ttttgccgaa aagctatact cgtcaagata tgattcagca
     3061 agcccgctac ttaggatctt tcgtccatgc ttgccataca catcaagata tcttggctgc
     3121 tacaatgatg aaagacctaa ttgctgagcc ttaccgtgaa gatttactgc cgaactttcc
     3181 ggctgttcgt caaggctgta aagacttagg tgcgttagcg gttggtattt caggttccgg
     3241 tccgacaatg tttgcgatag cccctgatct cgaacacgct caacgtttag tgagctattt
     3301 agagaaagaa tatctgcaaa acaacgaagg ctttattcat atctgtaaag tcgataatca
     3361 aggagcaaga gagctgaaat agccctctcg aacaaccata tcactaaata acttaaagga
     3421 ctgactccaa taccacttgg agcagccctt tttgattaat tttttgaaaa gtctatcact
     3481 tataaacaat cgaaggctat gccttacgcc aaattgtgcc gtttggggtg tcttcgagga
     3541 caatatttat cgctttgagc ttatctcgca cttcatcggc aaccgcccag tttttagccg
     3601 ctttggcttc gttgcgttgt ttgattaagg cttcgatttc tgccgtttca tcgtcattcg
     3661 catcaccttt taaaaacgca tcggggtctt gttccagtaa gcctagcacg ccggctaact
     3721 cttttaaacg aacggctaaa ccgttggctt ttgccatatc ttcggttttg agtttgttca
     3781 actcacgggc aatttcaaat aacaccgcta atgcccccgg ggtgttgaaa tcatcgtcca
     3841 ttgcggtttt aaaggcttct acatagtgtt ctccgatggc ttcgcccgac acatcacaac
     3901 cacgcagagc ggtatataaa cgctcaagag ccgaacgggc taaatcaagg ttgtcaatgc
     3961 tgtaatctaa taacgaacgg taatgagcgg ttaagaagaa ataacgtaac gattcggcat
     4021 cgtataagcc taacatatgg cgaatgctga agaaattgcc caaagatttc gacatttttt
     4081 ctttatcaat ggtaagcatt ccagtgtgta accaataatt gacataatcg ccaccgtgag
     4141 cacagcaaga ttgtgcaatt tcgttttcgt gatgcgggaa cattaaatcc gagccaccgc
     4201 cgtgaatatc aaaatgttcg cccaactctt tgctattcat tgccgaacat tcaatatgcc
     4261 agcccggtct gccattgccc cacgggcttt cccagcttgg ctcaccggct ttcgacattt
     4321 tccacaagac aaaatccatc gggtttttct tgatggattt aatttcaacc cttgcaccgg
     4381 cttgtagttg ctctaaattt tggcgggata atgcaccata tttctggaac gattccacat
     4441 caaacatcac atcgccatcg gctgcgacat aagcgtgtcc gttgtgaatc agtttttcta
     4501 ccatcgcaat aatttcagga atatgcttgg tggctcgtgg ctcaacgtcc gggcgaagaa
     4561 tgtttaacgc atcaaaatct ttgtgcattt cagcaatcat tctatccact aattcatcgc
     4621 aagtttcgtt attttctaac gcacgtttga tgattttgtc gtccacatcg gtaatgttac
     4681 gcacatagcg taaattgtag cctgaataac gtaaataacg ggcaatcaca tcgaaagaga
     4741 caaaagtgcg accgtgccca aaatgacaaa gatcgtaaac cgtgacgcca cagacgtaca
     4801 ttcccacctg atttgggtta atcggtttaa attcttcttt ttcacgtttt aaggtgttat
     4861 aaattttgag cataaagtcc tcttgtgtaa cattcagtaa caaatggggc taagtatagc
     4921 gaataagcgg tctgattttg agattttttt gcaatttggc gtaaactttt taataatagc
     4981 gagcgaaatt tattttccat tccattttta tttataaagg aacaaagaat gattacatta
     5041 cataccaatt tcggtgacat taaaatcgaa ttaaactttg aaaaagcccc gattaccgct
     5101 aaaaactttg aagattacgc taaagaaggg ttttataacg gcacgatttt ccaccgtgtg
     5161 attgacggct ttatgattca aggtggcgga atgacggcag gtatggctga aaaaccgaca
     5221 aaagatccga ttcaaaacga agcgaacaac ggtttaagca acaagcgtgg cacaatcgca
     5281 atggctcgca cttctgatcc gcattctgct tcgtcacagt ttttcattaa tgtggcggat
     5341 aatactttct tagattatcg ctcaaaagag atgttcggca gaagtgtagt gcaagattgg
     5401 ggctatgcag tatttggcga agtggttgaa ggtatggacg tggtcgataa aatcaaaggc
     5461 gtaaaaaccg gcaataaagg cttccaccaa gacgtgccgg ttgaagatgt ggtgattgag
     5521 tctgtaagcg ttgcctaatt ccgcaaatta gcctacaagc ggttgatttt tcacaatttt
     5581 ttgcatttct cagcgttgca aaaaagtagc agaaatcgac tgcttgtttt tatagaaatt
     5641 gatacagcca aataatcagc ggcattgtaa acacacaaag taaagtggtt acgccataaa
     5701 tcgcactggc ttttttggca tcttgcccgt aagccaccgc catttgggtc gccgttgcag
     5761 cagaagggct gatggtggct aagaatgtaa tcattaccac cgtttttcca ttctctatcc
     5821 agctcgcaaa accgccgagt ttaatcaccg ctaacaaaat cagcggaata aaaatcaagc
     5881 ggagaaaagc caccaagtaa atgcgtttat cggtaataat ctctttaaat ggaattgctg
     5941 caattaacat acctgccaca agcattgcta aaggccctaa catatgccct aaagttgaga
     6001 gcgtgtttgc aagcgtagcg ggcagtttga tatgtagcaa aaacaacatc ataccaatca
     6061 caatcgcaat aatgttgata ttaaagatca tctttctaaa cggcacattg cctttgccac
     6121 aaatcagcag ccgtaagtga gaccaaaaca acacattttg caccacaata aaagccgttg
     6181 aataaatcac ccattcattg ccgaacaggg aggcaacaat cggaataatt aagttgccgg
     6241 agttggaata aatagcggtg gcttgttcta tgttgtctaa tttcaacgct cttttgagca
     6301 gccaaccaag cagaattaac aagccgttta ataagacgga gattaaaaat gccagataca
     6361 agccgtttag tatttcggtg gtgtaatcta cttgaaaagc agtaatcagc attgccgggc
     6421 tgataatgta aagtccgata accgaaatcg gataactgtg ctccgatttg agtaatttag
     6481 ttttaacgat gaggtagccg agcagcacca tcagggttaa ttgtagaatt ttatcggcta
     6541 ataaaagggc tatttccatt atcaattaag agcgagtctc tcttctcctg cataaatggt
     6601 cgctttgcct ttgcgggcaa aagcaagtaa ggtaaattga ttttcacgag ccatattcac
     6661 tgccatttca gtggtcgctg agatggcaca caacatttca atgccaacgg ctaagcattt
     6721 ctgcaccatt tcaaagctgg cacgactggt tacaaacaca aatccaatcg gaaaatcagc
     6781 tttagcatac caaccgatta atttatccaa cgccacgtgt ctgcccacat cttcacgaat
     6841 ggcaagcagt tttccatccg gggtaaaaaa tgcagcggcg tggcttgcac cggtttcttt
     6901 cgataagaat tgagcatttt ctagctgtaa taaacaatcc tctaaaatca gtggattgac
     6961 gttctgcaag cggttatttt tcttaacaat tttgcaactt tgattaacct gctctaattg
     7021 ctctacaccg caaataccgc aacctgttct gcctgccatt gagcggcgtt tatcttttaa
     7081 tgcaacaaat tggcggctcg atatttcaag ctgaatttct actccatttt cttgttcaat
     7141 tacatctaaa ccatataatt ccgatggggc atttaaaatc ccttccgcca atgagaatcc
     7201 taaagcgaag tcctccaaat tagccggtgt tgccatcatt acagtatgag aaataccgtt
     7261 atataccaaa gcaaccggca cttctgaaat t
//
LOCUS       DANLUA010000130         7265 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLUA010000130 DANLUA010000000
VERSION     DANLUA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            36..455
                     /locus_tag="PXB44_001923"
     CDS             36..455
                     /locus_tag="PXB44_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL5282373.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            526..2082
                     /locus_tag="PXB44_001924"
     CDS             526..2082
                     /locus_tag="PXB44_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL5282374.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2140..2397)
                     /gene="rpsQ"
                     /locus_tag="PXB44_001925"
     CDS             complement(2140..2397)
                     /gene="rpsQ"
                     /locus_tag="PXB44_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL5282375.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(2397..2588)
                     /gene="rpmC"
                     /locus_tag="PXB44_001926"
     CDS             complement(2397..2588)
                     /gene="rpmC"
                     /locus_tag="PXB44_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL5282376.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(2588..2998)
                     /gene="rplP"
                     /locus_tag="PXB44_001927"
     CDS             complement(2588..2998)
                     /gene="rplP"
                     /locus_tag="PXB44_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL5282377.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3012..3719)
                     /gene="rpsC"
                     /locus_tag="PXB44_001928"
     CDS             complement(3012..3719)
                     /gene="rpsC"
                     /locus_tag="PXB44_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL5282378.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(3739..4071)
                     /gene="rplV"
                     /locus_tag="PXB44_001929"
     CDS             complement(3739..4071)
                     /gene="rplV"
                     /locus_tag="PXB44_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL5282379.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4082..4357)
                     /gene="rpsS"
                     /locus_tag="PXB44_001930"
     CDS             complement(4082..4357)
                     /gene="rpsS"
                     /locus_tag="PXB44_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL5282380.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(4388..5209)
                     /gene="rplB"
                     /locus_tag="PXB44_001931"
     CDS             complement(4388..5209)
                     /gene="rplB"
                     /locus_tag="PXB44_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL5282381.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5228..5533)
                     /gene="rplW"
                     /locus_tag="PXB44_001932"
     CDS             complement(5228..5533)
                     /gene="rplW"
                     /locus_tag="PXB44_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL5282382.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(5530..6132)
                     /gene="rplD"
                     /locus_tag="PXB44_001933"
     CDS             complement(5530..6132)
                     /gene="rplD"
                     /locus_tag="PXB44_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL5282383.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6148..6777)
                     /gene="rplC"
                     /locus_tag="PXB44_001934"
     CDS             complement(6148..6777)
                     /gene="rplC"
                     /locus_tag="PXB44_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL5282384.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(6803..7114)
                     /gene="rpsJ"
                     /locus_tag="PXB44_001935"
     CDS             complement(6803..7114)
                     /gene="rpsJ"
                     /locus_tag="PXB44_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL5282385.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2112 a         1547 c         1355 g         2251 t
ORIGIN      
        1 atttatatta atggagattc aacacggctg aacaaattgt tccttcaaat gaagctgttt
       61 taaaagcagt ccacgatact ttagttattt caacaactga tttacaaggt gttattactt
      121 acgctaacga ccttttctgc aaattaaccg gttttacccg tgaagaatta attggacaac
      181 cgcataatat cgtacgccac ccagctgtac caaaagaagt ttataaagat atgtgggata
      241 caatccaagc cggtaaaatc tggacaggta tcgtaccaaa ctgcggtaaa ggcggtgtag
      301 tttatgtggt tgataccacc gttcaaccaa tgtttgatga aacaggcaaa attacaggct
      361 atatcagcgt tcgccgtgta attaatgatt taatgaccaa ctttgaagca gtagaatcag
      421 caaaagaaat ctttgatgac ttctacggtg aataattaaa aaacaatgat tgcgtgtaaa
      481 agatgatctc ttttacacgc aattttttat tttcgaggag tttgtatgtc tctcattccc
      541 catatccaca ttgcttatgg ttcggagtct ggtaacgcag aaaagcttgc ccaacaatta
      601 gctatccaac cctttttact tccctactca cccacacttt ctacattaaa cgaaaccgat
      661 ttaagccaat tatctgccga gagtttattg cttattgtaa ccagctcctt tggtgatggc
      721 gaaccacctg aaaacgccga tgaatttgca gaaaaattgg aaaatttgac cgcttgtaat
      781 ctgcaatatg cgatattcgg gctaggtgat attacttatg ataaattctg tggctatagc
      841 aaacaactag attcattatt acaacaaaaa caagctaaag ctgtgattgc aagggttgat
      901 gccgatttaa actatcaaga aatttttaaa caatggctac ccctagtgga gcaggcatta
      961 cattcaacaa acaaagaaac gatccaacat tccctttctg ttcaagctta cggcgaggaa
     1021 atggcatacc aagcagaagt gttggaaatt aagcatttgg caaactcaag ccctgcggtg
     1081 tatcacattc gtttatcatt aaaagagagc ggtattttct atcaagcagg ggatttaatt
     1141 tatgtcaaag tgcagcaacc agacagacta ttaaatgaat atgcagaatg gtttaacgat
     1201 caaaatgcag ttgcattact acgccataaa gaactccgac tactaagtaa aaatgtactg
     1261 cgtgatattc aaaaaatttg cggcagtaat gaactcaaag atttaactaa aatttccaat
     1321 aaaaaagcat tggaacaata tgtctatggg cgggatttat tagacgtatt acgcgatttt
     1381 gacccaaaca aaagcgtgac attagccgat ttagagccaa ttttatctaa cttagccgca
     1441 cgagcctatt caattagctc ttgcggtaaa actcaccctg attatgttga tttatgtatc
     1501 cgccacgttt cgtatcattt aaacggtaga gattatcaag ggactgcaag tgggtattta
     1561 tccgaattac aagcgggcga ttttgtctcg atttttgcaa atgccaatcc gaatttccgt
     1621 ttacccgaaa cattaaccgc tccggtggtg atgattggct caggtactgg cattgctccg
     1681 catatcgcat ttttacaagc gttagagcag caatatcagg gggttgaaag ctatttattc
     1741 ttcggcgagc gttatcgtga taaagatttt ctgtatcaaa cggaactgga aaattactta
     1801 gcaaaaggca ccttaactca gctgtttact gctttttcac gtgatcaggc agagaagttt
     1861 tatgtgcaag atgccctatc gcaacaagca gagttagttt ggcatttaat tcagcaaggg
     1921 gcttatttct atatttgtgg tagcaaagca atgagtaaag caatagatgc ggaactcatc
     1981 aaaattgcag atcaaattgg aggcgatcct tatattgatg atttcaataa tattattgca
     2041 aaattggtcg cagaaggccg attaatgcga gatgtatatt agcaaatgaa aaaaactaaa
     2101 aacccagcaa aagctgggtt tttatttaat catagttcat taagcaacaa ctgctttctc
     2161 aactacacga actaaagtcc aagatttagt ttttgaaata ggacgacatt ccttaatttc
     2221 aacaacatca cctaatttag cttcattgtt ttcatcgtgt acatgaagtt tagtagtacg
     2281 acggataaac ttaccgtata acggatgttt aactgtacgt tcgatagcaa caacaaatga
     2341 tttatccatt ttgtcgctaa ccactttacc ttgtactgta cgaattttat cagtcattat
     2401 tcacccgcct tttcggttaa tacagtttta acttgtgcaa tactacgacg cacttgtttt
     2461 aactggtggg tttgttgaag ctgaccggtg gctgcttgca tacgcaattt gaattgttca
     2521 cctaaaaggt tcactaactc accattcagc tcttcaacac ttttattacg tagttcttga
     2581 gctttcatta catcaccgtc ttagttacaa atgtggtttt gaacggaagt ttagctgccg
     2641 ctaatgcaaa cgcattgcgt gcaacttctt cagaaacacc atccatttca tagagaactt
     2701 tacccggttg gattaaggct acccagtact caacgttacc tttaccttta cccatacgga
     2761 cttctaatgg tttttcagta attggtttgt ctgggaacac acggatccag attttacctt
     2821 gacgtttaac cgcacggctc atcgcacgac gagcagcttc aatttgacga gctgttaaac
     2881 gaccacggcc aatagctttt aaaccaaatg aaccgaagct cacttctgtg ccacccgcga
     2941 taccacggtt acggcctttg tgtactttac ggaattttgt gcgttttggt tgtaacattc
     3001 agtgattctc cttatttacg acctttgcca cgtggcgcct ttttaggctg tgccgctggt
     3061 tctttttcag attcaatcac tgcagccatt ccaccaagaa tttcaccttt gaagatccac
     3121 acttttacgc cgattacgcc gtaagtagtg tgagcttcag cagtactata atcgatgtct
     3181 gcacgaagcg tatgtagagg tacacgacct tcacgatacc attctgagcg agcaatttct
     3241 gcaccaccta aacgaccgct aacttcaact ttgatacctt tagcacctaa tttcatcgcg
     3301 ttttgaactg cttttttcat tgcacgacgg aacatcacac gacgttcaag ttgagaagcg
     3361 atgctatctg cgactaattt cgcatctagc tctggttttt tcacttcagc gatgttgatt
     3421 tgagctggaa cgccagcgat tttcgcaact gcgttgcgta atttttcaac atcttcacct
     3481 tttttaccga taacgatacc cgggcgagct gtgtgaatag ttacacgaat gctttttgca
     3541 ggacgctcaa tagtgatgcg tgatacagaa gcgttcgcta actctttgtt taaaaattta
     3601 cgtactttga agtcgccgtc taagttatca gcgaaatctt gtgtattcgc gaaccaagta
     3661 gagttccaag gtttaacaat acctaggcga ataccattag gatgtacttt ctgacccatt
     3721 tgttattctc ctacttaatt aacgatctga cacaaccaca gtgatgtggc ttgtacgttt
     3781 taaaatacga tctgcacgac cttttgcacg tggcattaca cgtttcatgc ttggaccttc
     3841 atcaacgaag attttagcaa ctttaagatc atcgacatct gcaccgtcat tatgctctgc
     3901 gtttgcaata gctgactcta atactttctt aacaagcgtc gctgcttttt tgttagtgaa
     3961 agttaagatt tctaatgcta cagaaacttt tttaccgcga attaaatcag caactaagcg
     4021 agctttttgg gcagaagtac gagcgtaacg atgtttagca atagtttcca tcttttacct
     4081 cttatttctt agctttctta tctgcggcgt gaccgcggta tgtacgagtc ggtgcaaatt
     4141 cacctaattt atgaccgatc atttcgtcgg aaacataaac aggaacgtgc tgacgaccat
     4201 tatggactgc gatggtcaaa ccaatcattg atggaatgat cattgaacga cgggaccaag
     4261 ttttaattgg ttttttatcc ccgctttcca ccgccttctc taccttcttc aacaagtgta
     4321 ggtcaaggaa aggacctttc ttgagggaac gtggcattgg tgtttacctt ttagttaatg
     4381 aattaaatta tttgccacga cgacgtacga tgaatttatc agtacgtttg ttgtgacgag
     4441 ttttcttacc tttggtttga acgccccaag gtgtaacagg atgtttaccg aagttacgac
     4501 cttcaccacc accgtgtggg tgatctaccg ggttcattgc agtaccacga acagttggac
     4561 gaatacctct ccaacggttt gcacctgctt taccaagaac gcgaagcata tgctctgagt
     4621 tacctacttc accaatggtt gcagtacact cagctaatac tttacgcatt tcgcctgaac
     4681 gaagacgtaa agttacatag ttaccttcac gggcaatgat ttgaacgtaa gcaccagcag
     4741 aacgtgcaat ttgaccacct ttaccaggtt ttaattcaac gttgtgtaca gttgtaccta
     4801 ctgggatgtt acgcattggt aatgcgttac ctactttaat cggagctgaa gcacccgctt
     4861 gaatttgatc accagcagtt aaaccttttg gtgctaaaat atagcgacgt tcaccatctt
     4921 tataaagtac taatgcgata ttcgcagaac gatttggatc gtattctaaa cgctcaacaa
     4981 cacccggaat gtctaattta ttacgtttaa aatcaattaa acggtagtgt tgtttgtgac
     5041 cgccaccaat gtgacgagtt gtgatacgac ctaagttgtt acgaccacca gttttagatt
     5101 tagtatctaa aagtgctgca aaaggtttac ctttgtgaag ctcagcgttt acaactttaa
     5161 caacgtgacg acgaccagcg gaggtcggct tacatttaac gatagccatt tattttatct
     5221 cctctgatta ctctgccgca ccttcaacga agtcaagcga ttgaccttct tgaagagtta
     5281 cataagcttt tttccagtca ctacgacgac caactttagc accacggcgt ttagttttac
     5341 ctttaactac tacggtacga acagaatcaa cttttacttc aaaaagttct tcaactgcat
     5401 ttgcaatctc aactttgttt gcatctaatg caactttgaa tacgattgta ttgccttttt
     5461 ctgcgttgtt tgtcgctttt tcagagatat gtggtgcttt aagcacgttt agcaaacgtt
     5521 cttgttgaat catgctaaca tctcctcaat ttgttttact gcatctacgg ttacaaccac
     5581 tttatcgaaa gcaattaagc taactgggtc aataccttgc acatcacgca catcaacttt
     5641 atataagttg cgtgctgcta agaataaatt ctcatctacg tttgcagtaa tgataagagc
     5701 gtcatttaac gccatatctt ttaatttttg tgctaatact ttagtttttg gtgcatcgat
     5761 ttcgaatttc tcaacaacca ctaaacggtc ttgacgaact aattcagaaa gaatgctttt
     5821 gattgcacca cggtacattc ttttgttcac tttttggctg tgatcttgtg gtttcgcagc
     5881 aaaagttaca ctacccgaac gccaaattgg tgatttaata tcaccagaac gtgcacggcc
     5941 tgtacctttt tgacgccaag gttttttacc tgaaccagac acttcagcac gagttttttg
     6001 agcacgagat ccttgacgag cacctgctgc ataagcaaca actacttggt ggattaatgc
     6061 ttcattgaac tcacgtccga aggtagtttc agaaacagtt agtgcgtttg cacctacaac
     6121 ttgtaattcc atctctatct cctagactta tgctttaact gccggtttta cgataacatc
     6181 accattagtt gcaccaggaa cggcaccttt aactaataat aatttacgct caacatctac
     6241 acgaacaact tcaagtgatt gaacggttac acgctctgca cctaagtgtc ctgccatttt
     6301 tttaccttta aacacacgac ctggagtttg gttttgacca atagaaccaa gtacacgatg
     6361 tgataaagag ttaccgtgag ttgcatcttg agtacggaag ttccaacgtt taacaccacc
     6421 ttggaagcct ttaccttttg atgtaccagt aacatcgact tttttaacat cagtaaagat
     6481 gtcaacattg atttcttgac ctaaagtgaa ttcttcacct tcagtagtac gaaattccca
     6541 taaaccgcgt ccagcttcaa cacctgcttt cacgaaatga cctgcttctg gtttagttac
     6601 acggctagct tttttagagc cagtagtaac ttgaattgca gagtagccat cgttttcaag
     6661 ggttttaact tgagtaacac ggttggcttc gatttcgata acggtaactg gaatagacac
     6721 gccgtcttca gtgaaaacgc gggtcatacc aactttacga ccgattaaac caatcattgt
     6781 aataacctct taaaattccc aattaaccta gactaatctg aacgtcaacg ccagcagcta
     6841 gatctaaacg cattaatgca tcaacagttt tttctgttgg ttcaacaata tcaactaaac
     6901 gtttgtgagt acggatttca tattggtcac gtgcatcttt attcacgtgt ggagaaatca
     6961 atacagtgaa acgttcttta cgagttggta aaggaattgg accacgaact tgtgcaccag
     7021 tacgtttagc tgtttctacg atctccgcag tagattgatc aattaaacga tgatcaaatg
     7081 cttttaagcg gatacggatt ctttggttct gcattagacc agagctccaa ttaaaaaatt
     7141 tagctaataa aaaaaccaaa ctaccactta acaaattagt taagggagct cagttaaacc
     7201 tatatagttc caaaattagg aacattgtat acactacaat atctgtagtg ccatttgata
     7261 aatga
//
LOCUS       DANLUA010000131         7231 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLUA010000131 DANLUA010000000
VERSION     DANLUA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..234
                     /locus_tag="PXB44_001936"
     CDS             <1..234
                     /locus_tag="PXB44_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL5282386.1"
                     /translation="YQQGYRVGSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSS
                     DVELGAEYTRDNLKRSGTKLKGNAFTANATYRF"
     gene            complement(312..1541)
                     /gene="serA"
                     /locus_tag="PXB44_001937"
     CDS             complement(312..1541)
                     /gene="serA"
                     /locus_tag="PXB44_001937"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL5282387.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(1578..2237)
                     /gene="rpiA"
                     /locus_tag="PXB44_001938"
     CDS             complement(1578..2237)
                     /gene="rpiA"
                     /locus_tag="PXB44_001938"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL5282388.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            complement(2382..3716)
                     /gene="glmM"
                     /locus_tag="PXB44_001939"
     CDS             complement(2382..3716)
                     /gene="glmM"
                     /locus_tag="PXB44_001939"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL5282389.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            complement(3742..5274)
                     /gene="ilvA"
                     /locus_tag="PXB44_001940"
     CDS             complement(3742..5274)
                     /gene="ilvA"
                     /locus_tag="PXB44_001940"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL5282390.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            complement(5535..6128)
                     /gene="slyD"
                     /locus_tag="PXB44_001941"
     CDS             complement(5535..6128)
                     /gene="slyD"
                     /locus_tag="PXB44_001941"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5282391.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            complement(6231..6371)
                     /locus_tag="PXB44_001942"
     CDS             complement(6231..6371)
                     /locus_tag="PXB44_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282392.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            complement(6443..7129)
                     /gene="aqpZ"
                     /locus_tag="PXB44_001943"
     CDS             complement(6443..7129)
                     /gene="aqpZ"
                     /locus_tag="PXB44_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL5282393.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
BASE COUNT         2060 a         1750 c         1352 g         2069 t
ORIGIN      
        1 tatcaacaag gttatcgtgt tggatccgat ttattacctt atgtaaaagt agatgccagc
       61 tcaagcaaaa tcggtaatga aacctatcgt ggctttggtt tcggtgctgg tgcaaaatat
      121 gcagtttcaa gcgatgttga attaggtgca gaatacactc gcgataattt aaaacgtagc
      181 ggcacaaaat taaaaggtaa cgctttcacc gcgaatgcaa cttatcgttt ctaatcttag
      241 cggtaaagtt ttctgaaaaa ataaccgctt gtaacacaaa agagcctgaa gtgattcagg
      301 ctctttgcct tttaatataa tactcgggct cgaatcgtgc cttcgatcgc ttttaaacgt
      361 tgtaatgctt catcaacatt gtcgctctct acatcaatta caacataacc gatagctgca
      421 tcggtttgta aatattgagc ggcaatattc acatttgatt ccacaaaaat ctggttaatt
      481 tgatttaaca tacctgggcg attctcgtga atatgtaata aacgtttggt attagtatga
      541 agcggtaagg atacttcagg gaaattcact gccgtcacgg ttgaaccgtt atctgaatat
      601 ttcacaaatt tattggcaac ttcggtgccg atatttgcct gagcttcaga ggttgaacca
      661 ccaatatgtg gcgttaaaat cacgttatca aacttgcaca atggtgacac gaacgggtca
      721 ttaattgatg ccggttcttc cggaaatacg tcaatcgccg caccacgcaa tgagccgtct
      781 tcaatccgag cggctaatgc gtctaaatcg accactgtac cacgagcggc attaattaac
      841 accgagcctt cttttaattg agcaatgcgt tctgcactca ttaggttttt agtcgagtcg
      901 atttccggaa cgtgtagtga aatcatatcg caggttgaaa gcaaatgctc taatgtcggc
      961 acttgctgtg cattaccgag tggtagtttg ttttcaacat catagaaata aacctgcata
     1021 ccaatggctt cggcaagaat actaagctgt gaaccgatat gaccatatcc cacaatacct
     1081 aatttcttgc cacgtacttc gtttgcacct gctgccgatt tattccacac cccacgatgc
     1141 acttccgcat ttgcagtcgg gactttacgc attaataaaa tcatctctgc cagcactaat
     1201 tctgccaccg agcgggtatt tgagaacggt gcattgaata ccggaatacc tcgtcttttc
     1261 gccgcattca aatcaacttg gtttgtaccg atacagaaac aaccaatcgc aattaatttc
     1321 ggagcttgag ccagtacttc ttcggttaaa aaagtacgcg agcgaatacc aacaaagtgt
     1381 gcatctttaa ttgcatcaat taattcttga ccgtctaatg cttttttatg cacttcaata
     1441 ttggtataac cagccgctaa gagtacatca attgcgtttt gatgtacgcc ctcaagcaga
     1501 aggaatttga tcttggattt atccaaagaa attttagcca ttttttgctc ctaagtattt
     1561 gtatttgcaa taataaatta ttctaaaata actgcacctt caggtgtgcc gacaatggtt
     1621 acattggcaa aacgttgtgc aaaaataccg ttagtcacca cgcctgcaat attattaatg
     1681 gtatgttcca ttttcaacgg ctccataatt ttaaagttat gtacatctaa aatcacattg
     1741 ccgttatcag tgaccacgcc ctcacggtat tccggcgaac cacctaaggc tacgagctga
     1801 cgagccacat aagaacgagc cattgggatc acctccaccg gcagagggaa agttgaaccc
     1861 agcacatcaa cttgtttgga tttatccaca atgcagataa attttttcgc taatgaactc
     1921 acgattttct cacgggtaag tgctgccccg ccacctttaa tcatcgcccc ttgtggcgtg
     1981 atttcatcag ctccgtcaat atacacatct aattccgtca cttcattggc actgaatact
     2041 tcaatgccga tatcacgcaa acgctgttcc gaggctttag aggcagcaac cgctccttta
     2101 atctcatctt tcattgaagc aagggcatca ataaaacagt ttacggttga accacttccc
     2161 accccaacaa tagtgtccgg ttttacaaat tttaatgcag cttgtgccgc aatttttttc
     2221 atttcttgtt gatccatttt tacctccact tgtagggcaa atcatcattt gcccacttcg
     2281 gcaaggcgaa aaaaattttc gccctactac atctattcta cacatatatg taacaagcgg
     2341 tcatatttgg caaatttttt accaaaaacg accgcttgtc attagttacg ttttactgct
     2401 tcaacaatct cttccgcaca gctttgagca agtacagcat cttcgcactc caccatcact
     2461 cgaattaatg gctcagtgcc tgatttacgc aataaaatac gccctttgcc tgccaagcgt
     2521 ttttccacat ctgccgctac cgctttcact tcgttacttt caagcggatt tgccgcccct
     2581 ttctcaaagc gaacatttag cagaacttgc gggaacagag gcactgcttt tgccaaatca
     2641 ttcaggctta atttgtggct tgccattgct gctaatactt gcagtgaggc aataatacca
     2701 tcaccggtgg tgtttttatc taacacgata atatgcccgg agttttcgcc acccagtttc
     2761 cagccttttt ctttaagctg ctctagtaca tagcggtcac ccacgttagc acgagagaat
     2821 ggaatggcta gctcttttaa agctaattcc aagctcatat tactcattaa tgtaccgact
     2881 acaccgcctt gtaatttgcc tgaacgtaga gcttctcggg ctaaaataaa tagaatttgg
     2941 tcgccatcaa ctttattgcc taagtgatcg accataatca aacggtcgcc atcaccatcg
     3001 taagctagac caacatccgc acctgcttct accaccactt tttgaagtgc tttaatatcc
     3061 gttgcaccac atttttcgtt gatgtttaaa ccgtccggct tagtaccgat ttcaagcact
     3121 tctgccccta gctcacgcat tacattcggc gcgatatggt aggttgcacc gttggcacaa
     3181 tccaccacaa ttttatagcc ttcaaggctt agttcagacg ggaaagtgcc tttacaaaac
     3241 tcgatataac gtcctgcggc atcattaata cgactggcac gacctaaatt tgctgaatct
     3301 acgcaatcca taggcttatc aagcattgct tcaatcgctt cttcaatttc atcaggtagt
     3361 ttttcaccaa ccgacgagaa gaatttaatg ccgttatcgt aataaggatt gtgagaggca
     3421 gaaatcacaa tgccggcttc cgcacggaaa gtacgggtta aataagcaat tgccggagtc
     3481 ggcatcggac caacgaatac tgctgataaa ccggctgcgg ttaaaccggc ttccagagcc
     3541 gattccaaca tataaccgga aatacgggta tctttaccga ttaatacttt tttcgtgcct
     3601 tgggttgcca acactttacc tgctgcccaa ccgagtttta atgcaaattc aggtgtaatt
     3661 ggaaatttac ctacttcacc acgcacaccg tctgtgccga aatatttacg agccattcta
     3721 attcctaaga ttgataaaaa tttactttaa aaaatattga tatgccggac tttcgctcac
     3781 attttggaag cggtaaccta gctgttctaa atgtcggttt aattctactt catcgcctct
     3841 gtttaatgca aatgcggcaa gaatatcgcc ataatcggct ccgtgagctc ggtaatggaa
     3901 taacgagata tcccagttag tcagcatttg taagaatttt agcaatgccc ctttctgctc
     3961 tggaaattca aagctataga gttgctcata ttgaatggag cttgctctac cgcctaccat
     4021 ataacgaata tgggttttgg caatatcatc gtccgataaa tctgccacat catagccgtt
     4081 ttgttgcaga tctttaataa tctctacttt ttctgccgag cctgaaattc gcacccccac
     4141 aaaaatacag gcttgtttat catcggaatg gcgatagtta aattccgtta ccgcacggtt
     4201 gcctaaaatc tcgcagaatt ttaagaaact acctttctgt tcagcaatgg tgactgccaa
     4261 taaagcttca cgtttctccc cgatttcaca acgctctgac acaaaacgta aggtgtggaa
     4321 attcacattc gccccggaaa gaatgcaagc taagttttta tcttgtaatt gatgctcttt
     4381 cacatatttt ttcaaaccgg caagggaagt tgccccggac ggctcggaaa tcgcacgcac
     4441 attttcaaaa atcaacttca ttgaggcaca gatttcatca ttatccacca acaccacatc
     4501 atcaacatat tgctgacaca ggcggaaagt ttcatcgcca atgcgtttta ccgcaatacc
     4561 gtcagcaaaa agcccaaccc gttctaaatc ggtcggttgc ccttgcttta aggcgtgata
     4621 taagcaagca gaatctttag attcgacccc aatcacttta atattcggca tcacctgctt
     4681 aattaaaacc gcaatgccgg caattaagcc gccaccgccc actggaataa atacataatc
     4741 taaatccggc ttttgttgca ccagttctaa gccgattgtg ccttgccctg caatcactgc
     4801 cgggctgtca aacgggtgaa tgaaggtcat acccaattcg gtggaaagct caatcgcttt
     4861 cgccttagct tcatcaaaat tcgccccata aagcaacact tcaccaccat agccacgtac
     4921 cgcatcaacc ttaattgccg gtgtgttttg cggcattaca atcaaggctc ttaaacctaa
     4981 atgtttggca gaaagtgcca ccccttgtgc gtggttgcct gctgaagccg caatgactcc
     5041 agcctctttt tgtgctttgg ataaatttga gatcatcgca aaagcacctc gcaatttaaa
     5101 gctgtgaacc ggctgtcggt cttcacgttt gataaaaata gagttgccta accgctcaga
     5161 cagtttttcc attttctgta aaggggtcac ttgcgctaag tcataaatat ttgagcttaa
     5221 aatcgagcgt aaatagtcga tagcggattg ggaatgttgt gtttcgttca tcattttgaa
     5281 aggttattta ttgttagatt gagataaaga cattggtaaa tttccaagga aaattgaccg
     5341 cttgtttggt tatttagcaa tggctttaac agggaatatt tcctctcgcc cgtttgcggg
     5401 agagagacag cttaaaattt cgttcagaaa ttttaagcag agagagggga aaccttggat
     5461 atccccctct ccctccgcaa atcttcgatt tgctccaccc tctcccgcaa gcggggagag
     5521 gggaaatcac aaagttagtg atgaccaccg caaccacagc caccgtgtcc gtgatgatgg
     5581 tgatgatcgt ggtcgtgacc gccgcagcaa ccatcgtgat cgtgatgatg atcgtgccca
     5641 ccacaaccgc aaccgtgttc gtgtccgccg tgaacgtgtc cgtgtgcgat ttcttccgct
     5701 gtcgcttcac gaatgccaac cacttccaca gagaaaagta attcctgacc ggctaacata
     5761 tggttaccgt ccaccacaac ttcatcgcca tctacttcgg taattaccac cggtagcgga
     5821 cctaaatccg tgtccgcaat aaaacgcata cccacttcta attcatcgac gcccataaat
     5881 acatctttcg gtacacgttg aaccatatta tcgttgtact cgccgtaacc ttcttccggt
     5941 ttcacacgca cttcaaacac atcgcctaca gctttgcctt caagggcttt ttctaagcct
     6001 tctaccaagt tgttgtgacc gtgtaaatat tctaacggtt ggtttaccgg tgcttcatct
     6061 actaatacgc cttcttgagt acgcacttga tatgcaatgc ttggcactaa atcttttgta
     6121 atgttcatat tgaatccttt aattcatcgg ttttataaaa tagcaagcca ttgtaacgct
     6181 ttattttata tttgcaatct atctggaaat tttaaccgct tgtcagtaaa ttagtcgata
     6241 atatcagggg ttacggtgtc taccacatca actgcggttc ccaccactcc accaaccacc
     6301 gaagttacca aacaaccact taggaaaata gttgctgaaa tcagcccaca tactttgatt
     6361 aattttttca ttttaaaatc ctttcaaaaa caaaaaacag ccctaatcaa gactagagct
     6421 gcatcatatt tattcgctta aattattttt cttcagcgat aaagcgataa actaccgcac
     6481 cgataattgc accgacaatc ggagcaaccc agaacaacca taattgctca atcgcccagc
     6541 tgccttggaa taacgctaca ccggttgaac gtgccgggtt tactgaggta ttggttaccg
     6601 gaatgctgat taagtggatt aaggttaaag ccaaaccaat agcaatcgga gcgaaacctg
     6661 ctggagcacg tttatcggtt gcacccataa tgatgattaa gaagaatgcc gttaatacca
     6721 cttcaattaa taatgctgct gttaagctat agccgtgtgg cgaatgctca ccataaccgt
     6781 tgccggcaaa gcctgctgta gcatcaaaag tcggaacacc tgaagcaatg gtgtataaca
     6841 ctgcaccggc agcaatcgca ccgattactt gggcaatgat ataaggcact aaatctttag
     6901 cgttaaaacg accgccgact aataaaccga ttgaaaccgc cgggttgaag tgaccacctg
     6961 aaatatgacc gactgcatac gccattgtta atacggttaa accgaaggct aacgaaacgc
     7021 ctgcataacc gattcctaat tcaggaatgc ctgctgctaa aactgcactg ccgcaaccac
     7081 caaacactaa ccaaaatgtc ccgaaaaact cagcaaaata ttttttcatt ttttatcctt
     7141 accctaaagg gtgaatcaca cataagtgta ttaactttcc gaatgctata aaacatagcc
     7201 aataaaatat gagtatacta aaaaaagtaa a
//
LOCUS       DANLUA010000132         7123 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLUA010000132 DANLUA010000000
VERSION     DANLUA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(83..2650)
                     /gene="glnD"
                     /locus_tag="PXB44_001944"
     CDS             complement(83..2650)
                     /gene="glnD"
                     /locus_tag="PXB44_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL5282394.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            2762..3739
                     /locus_tag="PXB44_001945"
     CDS             2762..3739
                     /locus_tag="PXB44_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL5282395.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            complement(3796..4878)
                     /gene="serC"
                     /locus_tag="PXB44_001946"
     CDS             complement(3796..4878)
                     /gene="serC"
                     /locus_tag="PXB44_001946"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL5282396.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            5210..5890
                     /locus_tag="PXB44_001947"
     CDS             5210..5890
                     /locus_tag="PXB44_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL5282397.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            5902..>7123
                     /locus_tag="PXB44_001948"
     CDS             5902..>7123
                     /locus_tag="PXB44_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL5282398.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGA"
BASE COUNT         2004 a         1408 c         1412 g         2299 t
ORIGIN      
        1 caacctctct taactcgtga tttaatttac ctaaaacgtt gaaaaattta ccgcttgcag
       61 tcttcactcc aagcggttgt tttcattcac tatctagttc ggtaattaga atggctttta
      121 gttctaactg ttcttgttcc gataaagctt ttccttgatc attactgacc acaaagaaat
      181 cctctaccct ttcaccaata gtcgtaattt tcgcattaat taaatttaat cggagccggt
      241 taaaaatata gcctatttgt gccaacaacc cttctctatc caaggtaaaa agttcaaatt
      301 cggtttgttg ctttgatgac ggaggaagaa aacgcagccg agttttacgc ttaaatgaca
      361 aatgtttcac cggttttttg gtaaatttta tgcctgtttc agttaagctt aacattttca
      421 tcatcgcctg ctgaatttgc atacatctct catcggttaa atcatcatca ccatcacgct
      481 cacttacaat aaagctgtct aacactacac cattttcagt agtaataatt tgtgcatcgt
      541 gaatgctaat gcgtttttgg ctgagtaatt gggcgattcg agcaaacagt tgaggttgat
      601 cttggcaaca tacaaaaatt tctgtaccac cacgggcata ttcattactg attaaaacca
      661 tcggcaaccg gcgagttttt acataatgca gagcgtgcca taccaactga gtcggtgtat
      721 ggtgaagaaa ataacgctcc gggcaaggtt gccaaaatgc gtttaatgct attcgctcat
      781 cagctgaaag cacgaacttc atcagttcta atgcttgcaa gcgatgctct tgtgccactg
      841 cttgataatt caaattttga gccagttgct gtagcgtaaa ttgatataat tgggtaaaca
      901 gcgaggcttt ccaatcattc cagaggctct cactggttgc acaaatatct gcaaccgtta
      961 aacaaagtag tgaagataaa gcggtctgat tttgcacttt ttttgcaaat tcgctgatga
     1021 tttgcggatc gtggatatct cgtctttgtg cggtgatcga cattgtcaaa tgctctgcca
     1081 ccagccaaac catatcattg atttgtgctt gagaaaaacc gtgtagttcc gcaaattctc
     1141 gcatatccag cgcaccaact tgagcgtgtt cgccctctct acccttgcca atatcgtgga
     1201 aaagagccgc aagatagagc aacggtttct cagaaagcgt tgcaaatagc tgatgacata
     1261 gtgggaaaaa cgcccgactt ggatcttcgc taaaattttc taaatttaac atgactctga
     1321 ccgtatgctc atcgactgta taaatatgga ataggtcgaa ttgcattagc ccttcgatcc
     1381 ccttccattg cggtagataa gcacttaaaa aacccagttg gtgcattgga actattgctc
     1441 ggcttatgac tttcggttga gcaaaaagct gtacgaattt ctctcgcatt tcaggcaaaa
     1501 agcacatagg ctcgtgttgt tgctgtaaac acagtctcaa ctgacgtaag gtgtcggcat
     1561 tcgctattaa cgtagggtca ttagtaagat gaaaaaagag atccaacatt gttgtgggtt
     1621 gggttttaaa tattttgcgt tcgaccacaa acaattgctg ttcttgccga taaaaatact
     1681 gatcaagcgg ttgtttttgt gcattttttt gcaactcgtt tacataggct tgttcaaaac
     1741 tgtttaacaa taattggctc agttgcgaaa tagattttgt ggcttgaaaa taatctcgca
     1801 tcattaattc caccgcttga tttgcttcgc cttggtagcc taatatttca ctcagttgta
     1861 attgtctgtc aaaccgaagg cgattatcgt aacgtttcag ttgcaaatga agtgcaaaac
     1921 gcatccgaaa aagtaccgct tgtgctgcga tcagctcctt atattcttcc gggtaaagta
     1981 gccctttatt gaggagatcg acaaaagaag atacaccata atgccgtagc ataatccaag
     2041 agattaaatg cagatctcgc aatcctcccg ggctatgttt gagatctggc tcaaggttat
     2101 agctagtatt atgataacgt gcataacgct catttttttc cgccattttc gctctaaaaa
     2161 acgctttaat atcccagaaa tccggctgat aaagtgcttc ttttaatctt agccaaagcg
     2221 atttattacc caataaaaat cgcccttcca acatattagt ggcaaccgaa atttctgctt
     2281 tcccaatctg aatacattct tcgagcgtac gcacactggt gccaagttgc agtttgctgt
     2341 cccaaagcaa ggcaaatagt tcgtttaaac gctgctgaat agcctcttct agcggttttt
     2401 cagttaacac taagatatcc agatccgaaa gcggaaacat ctcctcacgt ccgtaccccc
     2461 ctaccgctaa tagggtaata tgttcatatt gagcgagtcc aaaatcttgc caaagtgctt
     2521 gcagtagttg gtcataaaat tggctacgtt gtgcaagtaa gctgagtaca ttttcggatt
     2581 gaaatgcttg ccgctgttgc tccgtaaatg ctgataaaag cgtttttaat tcgttgctgt
     2641 ttttcatcat aatctcttcc taaactggca aaataatagg tcatttattt tcgtttgcct
     2701 atatttattt tggctaaaat gcttataatg cctttatgtt tttcatctaa aaggatagtt
     2761 tatgacactt gctgttgccg ctattatcgg tggcttaatt atattagttt ggagtgcgga
     2821 tcgttttgtg gaaggggcag cctctacggc tcgccatttt ggtatgacac cattgttaat
     2881 cggtattgtg gtcattggtt ttggtacatc tgcaccggag atgctcgtat ctgcatcttc
     2941 tgcattaagc ggatcgccgg gtatcgctct cggtaatgcc tacggttcta acattaccaa
     3001 tattgcgctg attttaggtt taaccgcaat aattaaacca ctatcagtta attccgatgt
     3061 gttaaaaaaa gagttgccga ttttactggc tgtcaccctg ctttctgctt atttggttta
     3121 tgatgcaaac gtcactcaac ttgatgcgat tattttactt gctgtgtttt ttgtatatat
     3181 ggcgtggaca attattaccg caatgaaaag taaaaatgat gcattaagct tagatgttga
     3241 tgcagaatta accaatagtg ctaagatggg attaggaaaa tccattcttt ggctaattat
     3301 tggtttagta tcgctagtaa ttagttccca attcttagtc tggggagcag ttgaagtggc
     3361 taaatttttt ggtgtgagtg atttggtaat cgggcttact attgtggcgg taggcacctc
     3421 actacctgaa ttagcctctt ctattgcagc tgctcgtaaa ggagaagtgg atttagcttt
     3481 aggtaacatt atcggttcta acttatttaa tacgttagcc gtggtgggga ttgccggtgc
     3541 tatagaacca atgcaagttt cagcggaagt gttttctcgc gatgtcttga tgatgtcggc
     3601 tctcaccttc ttattattca ttttcgggat caacctctat cgtcggacag aaggtggcag
     3661 aattaatcgg ttagagggct tattactctt tgccgcttat gtaggctata atttctattt
     3721 gtttaaaact gcgggctagt gccaaattca aagagccaca aacgtggctc tttaatttat
     3781 attcttaagc ttgaattaag cattacgttg ttcaaatacc ttcataaatt caatgagtgc
     3841 ttttacccca tcaatcggca ttgcgttata aattgaggca cgcattccac ctaatacttt
     3901 atggcctttc aatgcctgta aaccacaagc ggtcgcttct gccacaaatt ttgcatttag
     3961 ctcgtcatta cccgtggtaa aggttacatt cattaaggaa cggttttcgt gtgctaccgt
     4021 attgtaatag aatgttgaac tatccaaata ctcataaagc aatgtagctt tagcaagatt
     4081 acgttcttca gtagctttca aaccaccctc tgccagtaaa tgttggaaca ctaaagaaca
     4141 taaataccaa gcaaaagttg gcggggtatt aatcatcgaa tcagcatctc gctgagttgc
     4201 ataattccaa atagatgggg tatctttgcg tgcattgcca attaaatcat cacgaataat
     4261 cacaatcgta atgccagcag gccctagatt tttttgcgct ccggcataaa ttactccaaa
     4321 tttgttgata tcaatccctc gggctaagat attagacgac atatctgcca ctaacggggt
     4381 atttccgaca ttcggaatat cgaaaatttc taccccactg atggtttcat tggtgcaata
     4441 atggatataa tcatattgct ctgcaatatc gctgaaatct aaacgactca ctttaagttg
     4501 cggttcagtc tctaaaatat tgatttcatc aatttccgca aagttacgtg cttcttttgc
     4561 tgctgttgcc gaccaatgcc ctgaattaag gtaaagcgct ttgccttttt taccgattaa
     4621 attcattgga atggctgcaa attgccccct tgcaccacct tgaagaaata gaatacgata
     4681 attgtccggc acattataca gcttgcgtaa atctgcttca gattgtgccg ccagctccat
     4741 aaatagcttt ccacggtggc tgacttccat cactgatgtg ccttggtcta gccaatttaa
     4801 taattcttgt tgcgcttttt cgagtacttt tttcggcatc attgccgggc cggcactgaa
     4861 gttatatatc tctgtcattt ttcacctcgt atagtaataa tattgattct attctatctg
     4921 aaaaaaagcg taatggtaga ataaaaacag aaaaattatt ttagcaaact tgcataaaat
     4981 actcttttta aaagcataat actctttcgt aacatcaccc aaataaggaa taagcgatat
     5041 aaaaaaacaa gcataaattt cttccatttt ttgcaaaaaa attattaaaa caaaccgctt
     5101 gtcttaaaaa cataatttaa tttcgtgatc tttactaata catcaagtaa aatacgccgg
     5161 actttacagt aagaagtcat tgtcaatatt atcactatgg agatatttta tggcattgaa
     5221 taacatttta ggtttattcg cccagtcccc acttaaaccg ttacaaaaac actcaaataa
     5281 agtgactgaa tgtagcgagt tattagaatc tttttttgaa gcgacttttg aacatgattg
     5341 ggaaaaagca gctgaagtgc gcggtaaaat tgttgattta gaacgccgcg cagatgccct
     5401 taaacgtgaa atccgcttaa aactgcctcg tggtttattt atgccagttg aacgcggtga
     5461 tttattagaa ctagtcacac aattagataa gttagcaaac tattcaagag atatttccgg
     5521 tcgcattatt ggtcgtaaat taatcattcc taccgaaatg caaccacttt tcaaaaaatt
     5581 cctttctcgt agtattgatg ctagccgtca ggtacgcaaa gttttggatg aaatggatca
     5641 gttattagaa actggtttta gagggcgaga gcttgatttc gtcaataaaa tgattcttga
     5701 acttgaccaa attgaagatg ataccgacca atatcaaatt acattacgtc atacacttct
     5761 tgagttagaa aaaaacctga accctattga tgtgatgttt ttatacaaat gtattgaaag
     5821 aatcagcgtt ctagctgacc aagcccagcg aattggttct cgtattgaac ttatgctggc
     5881 gaagtcatag gagaaaatta aatggaatta ctcaatcaat atggctcaat actggtgatt
     5941 attactgcta ttgtagggtt tatgatggct ttcggtattg gtgctaatga tgtttcaaat
     6001 gccatgggaa catccgtcgg ctctggaaca attacgatta aacaagctat tttaattgct
     6061 atggtatttg aatttgctgg tgcttatctg gcaggcggtg aagtcgcaga tacaattaaa
     6121 agtggcatta ttgatccggc acaattcgcc aatagccctg atatcctggt acttggaatg
     6181 atggcatctc tctgtgctgc cggaatgtgg ctaattattg caactaaaat gggttggccg
     6241 gtttcagcaa ctcacacaat cattggtgcc gttattggtt ttgcattaat cactattggt
     6301 tcagatgcaa ttcaatggga tgcattaggt ggaattgttg ggagttggtt tgtgacccct
     6361 attcttgcgg ggatcgtagc atattcgatc tttattaact cacaaaaact gatctttaac
     6421 cgctcaaacc cattcaaaca agcgaaaaag tatggcccga tgtacatggg aattactatt
     6481 tttattttag ttattgtaac cgtgtcaaaa ggcttaaagc acgtaggatt acatctagat
     6541 acagctgaaa cattgggaat ttcattcgcg cttgccttac ttgcttttat cattggatat
     6601 ttttatttcc gcagtaaaag ctttgcaatg aaagctcgtg cagaagagaa aggcttcgct
     6661 ggcgttgaga aaatttttag tattttaatg cttattacgg cctgtgctat ggcttttgct
     6721 catggctcta atgatgttgc taatgctatt gggcctctag ctgctgttga atctattgta
     6781 agaagtggtg gaatgattga aggtaaaacg actttagccc cttggattct cccactaggc
     6841 gccttaggta tgatgattgg tttagctatt atgggcaaga atgtaatggc tacagtaggt
     6901 actggtatta ccgaccttac tccaagccgt ggctttgctg ctcaatttgc ctgcgccgtc
     6961 acggttgtca ttgcatcagg tactggttta ccaatatcaa caacccaaac gcttgtagga
     7021 gcagtattag gtgtgggatt cgcccgtggt attgctgcac ttaacttagg cgttattcgt
     7081 aatattgtta tatcatgggt aattaccttg cctgtaggtg ctg
//
LOCUS       DANLUA010000133         6886 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLUA010000133 DANLUA010000000
VERSION     DANLUA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..4453)
                     /gene="mukB"
                     /locus_tag="PXB44_001949"
     CDS             complement(<1..4453)
                     /gene="mukB"
                     /locus_tag="PXB44_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL5282399.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGF"
     gene            complement(4629..5345)
                     /gene="mukE"
                     /locus_tag="PXB44_001950"
     CDS             complement(4629..5345)
                     /gene="mukE"
                     /locus_tag="PXB44_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL5282400.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(5394..6737)
                     /gene="mukF"
                     /locus_tag="PXB44_001951"
     CDS             complement(5394..6737)
                     /gene="mukF"
                     /locus_tag="PXB44_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL5282401.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
BASE COUNT         1783 a         1646 c         1412 g         2045 t
ORIGIN      
        1 caaagccttt taaaccaaca acgtgaacat actcttggtt attggtgatt ttacgcacca
       61 atttataagt tgtgcctcgt tcagggctaa tattttcagg ggctgcaatt aataactgca
      121 tatccaaacg ttcgcagagt tcaaatagcg tattaattga ggtagcatct aatcgagccg
      181 cttcatctaa gaacaataag cgagccggta gaatatcttt ggcacgcata cggcgagctt
      241 cttcttccca gctttgaact accattaaga gaatcgacat acctgtaccg atcgcctcac
      301 cggtcgataa tgcactactt tctgcacgca tccagccgtc tgcaccacgg aaggtttcca
      361 cttctaaatc taagtagtta cggtaatcca ataactcttc accaatggtc tgtggtgtac
      421 gttgccccat ctcaatatga gggttcaccc gtttatacaa catcgctaag gcttcggaga
      481 agctcagttt ttggctgtcg aataaatctt tgtgttcctc acgattattg gataacgcat
      541 tgagtaaaat agcgtgcgta tctcggatat tcaccaccaa acgcacccct tttacctgac
      601 cgaatgcaat attttgtaaa ccttggttca gttgcaaaat gcgttgttgt tcacgctgaa
      661 tggttttacg taaaatattt gccacacttt ccgcactgat cgccagtttt ttctcacgac
      721 cggttaattc attggttaag cgtgaaagct cgatttccat ctgctcaatc gcatcaatcg
      781 gatcatcggt tttgataatg tcttggcgaa tccgctcacg caaatgctga taaaccgcaa
      841 taaagaacgc cactttgttt tccggcttgc gactatcttc agaagcccgc aagctatcac
      901 gcaggtattc attatccgcc acggcggtgc gtaatgcccc gagagcttta tccgaaatcg
      961 aacgtaattc ttcagccgat tgataagcca attcacggcg atttaaacgt ttttccacat
     1021 cgctgttacg agataaacgt tgcacaatac accagctggc ttttgcctgc accaccaatt
     1081 cacgttgtgt atgataatca cgttcggctt tacgtaaggt gcggttaagg ttatccattt
     1141 ccgcctcgat caccgcaatt tgtttatcta aataaccttt gcgggaacgt tgctggctta
     1201 aacgttgttg caattcatca cggcggaatc tggctcgctc ttccgctccg ctatcaccac
     1261 gcacgcccaa atcgtcaatt tcacgaatta actcttgtag catttggttt ttcgcctcat
     1321 aagaactgcg taagccggtc aatacttggt tatattgtga aagttgagct tgagcttgac
     1381 gcagctgatc acgagcctgt tcacgctcac gttgtgcatt ttccaaacgt tggcgtagct
     1441 gttcggttaa tgcagagcct tctgtaccca ccgtttcttg atagctaaaa tggaggcggc
     1501 gattcattac atcagccaag ctgaacactt tttgctgtaa cactttttgc tcggcaactg
     1561 aacggcggta atcggcttcc aactgttcaa ataaagccgg atcacttttt aatgcggctg
     1621 caatcggctc aagttggctt aatgttgctc caaattggcg gataaacatt tcatcttctt
     1681 ctgccaattc taattgctca cgacattcct ccgctcgtgc ttccagcgtt tcatcggcta
     1741 ataaattcgc aagcggtaga attttattca acatttgcaa ctgggctttt acgctttcta
     1801 attggtgacg taattgctgt tcgttaccgg ctgattgtgc caattcacgt tcaatttctg
     1861 cccgttctgc cgcaatctct tgcattaaca tttcagggtt ttcttggaac gctaaattta
     1921 agtgcgtgcc gataaattgg cttaagtgtt gatgtaaacg ctggcatttc tgcacatcaa
     1981 aggcacgttc agcgtgtttt tctgcggttt catcacgctc tgcttttaaa gtcgctaaat
     2041 gtttttcacg agcagcacgt ccgaataacg gcacttcagg gaatttagaa taacgccatt
     2101 ggcgatcgga gactttcacc accacgcctt cgcctaattc ctcggcacta aataccgcat
     2161 cgtcaaaggc ggacggatca ccttcaatta aataaagatc acttgggcaa tcgtctaatt
     2221 tctctagctg tgttttaacc gcctctaaat cacgcactac aatagcgtgg cgagcctcgc
     2281 cgtataaggc ggagaaataa ggcgcatctt caattgatac atcatcatat aactcagaga
     2341 gtaacactcc accaaaacgt tctgccaact ggtttaagcg ggcgtcttcc gagccgtccg
     2401 gctggcttaa gcgattaatt tgctcatcaa gcatttgttc tttacgagcc aattcatcac
     2461 gagcaagggt ggcttcacgc tctttgctca gcatattttg cataaactgc atcactgatt
     2521 ggctggcttc aaacttctcg ccacattgct cttctaaacg agtcagagcc gattgtgccg
     2581 tatgccaagc cggtgcattc ttcgctaatt gctgatattg ttgattaagc tgttcacgtt
     2641 gttggcgttg ggttgaacgt tgttctacaa agtccgaaag ctctgcctct aaatcttcta
     2701 ggcgagcttg ctgctcttca aaataggcgt ctaattcttc cgctgaattg agttgttgtt
     2761 gcgatttttg gttaaattcc gccactaaac gctgggcatt ttgctgttgt tgcaaacgct
     2821 gttctaaatc gttgagtttt tgacgtaaac tcaccgcttg ctgagcttgc attttttgct
     2881 cagggaaggc agaaagtaac gcacgagcct cttcccacgc ttgcaagcgg tcaatttcac
     2941 cggaaatttt gcaaaccaat tcaaacgctt tttcaaattg ggttttcgcc atatccgaca
     3001 cgcttaaacg ttgttccagc tcaaatactt gatcggtaag atcttcggct tgagccacaa
     3061 attctgcgtg gtaatcttca atattattta aatctaaatt cactaagccg gttagctgtt
     3121 tggctttttc taacgcattc accgcttgtt ggtattgcaa tgcacgagtt tgctgtgcat
     3181 ctaaggcttg ttggtaatcc gccatttgag aacgcaattc ttcaacgtga tcatctgcct
     3241 catccgcacg agcctgagca ttttcggcaa tttccgcaat ttcttccaat gcctcttgct
     3301 gttcttccag cttaatattc agctcttcaa cttcgtcttg gtagcgttcg attttctctt
     3361 ggtggcgtaa cgcattcatc actaaattta gatgatcgtt cgcactattg taatccgcct
     3421 ctaacgccag ctctgcttcg gcaatatccg cactttcacg gctaaattcc acaaagcgtt
     3481 gctgttcgag caaaattttg gattttgccg cataccactc tttgcgttgc tctaaggctt
     3541 gttgtacatt gccacggcgt tcattcgcat tacgcatata atccgccgac acataattgg
     3601 tcgattcggt aattaagcgt ttgaacatat cccgatccga ttgcgtgacc ttaatcgcct
     3661 ctaaggtcat acggttttca cgcagagcgg attccatatc ttggaacgct tggcgaacgc
     3721 ccgtattttc cggcaacaag taatcacgca aggatttggt aatcacactt gaaataccac
     3781 cataaagcga ggcttcgatt aatttgtaga atttgctacg gtcggctgcc gaacgaaggc
     3841 gttttgggat tacgcccaaa tcgaacataa aactgtggta atcagtaatc gaatggtact
     3901 gtttgaattg gacttcgctg ttctcaaatt gatctttgag ttcatttaac gccagcactc
     3961 gagccttatt ccccacttgt tcggtcagta ggctgataat gctttgctct tgaggaatat
     4021 tctggagcga gaacgaacga atatcgactt ttttatcccg tccgacaatt tgttgcaaac
     4081 gcacgccggt aataatccgt tgcccacggg aattgaatga ctctaacacc gcatagcaaa
     4141 cacccgcttt taacttaccg tataagcctt tatcacgaga accggcggtt gagcctgctt
     4201 cagtcgtatt acggaaatta agcaaggtta aatctgggat caaggcggtc acaaaccctg
     4261 ccattgtggt ggacttacct gccccgttac cgcctgaaag cgtggtcact aagtcatcta
     4321 aatcaaaggt tcgagcaaaa aaaccgttcc agttaatcag cgttaaagag agaaatttac
     4381 cgcgttgtac atcattcggg cgtgcaagcg gtgcaatttg aggattattt tgcaattctg
     4441 tggtatcaat cattatgttt tctcactaat tgtcctagca cgctaaagcg tactaggtta
     4501 ggtataggtg agtagctccg ctactctaat cgcaacctca gccttggagg gctgctttat
     4561 gcttaaccta gttcggcttt accgtgtcta gggcagactc attaaatgct gaatctattc
     4621 gaatgaaatc actcattttc tccgtcaaag ggggcttcat ctgcaatatt ttcatctatt
     4681 tcaaccgctt gttcgtttaa caacgcaggg gttgtcgctt caccatcacg aattaagcga
     4741 agctgtgcct ctcttgggtc atcaccggta cgcacatcag ccccaaaacg gaagaccgat
     4801 tctgaaatca caaatttttt gctgttttgc tcgcctactc gggtaataat gccgatacgg
     4861 gctaatctgc gtaacgctcc gccggctttt tctgccagct tggcacggtc taaatcggaa
     4921 ccggtcgaac gtgggtttac cgcttttaat aatttgcttt catcggcaag actgagtaat
     4981 tcttcataca catcatcttg gctgaaaatc ccctgctgag ccaaacgttc cggacttaaa
     5041 tagaggtagc acaacacctt acccactaac atttccattt ctgacattgc cgaacgggca
     5101 attagtgtcg aggctttcgg gcgtaggtag aaaaagcctt ccggtgctcg aatcaactca
     5161 acgtgataac ggcggtaaaa actctctagt tccgcttgaa aatccatcaa aaagacgtgt
     5221 tggtcaagct gctcggtatt gatatggcga ccggctcgta attggctgtc tagctccgga
     5281 aaaatcgggt tggcaatcgc aagtgccagt ttcggggaaa tgaaatcttg gatattctct
     5341 gtcattgtca ttctcttcaa ttttgcaaaa ttctgggtaa aaacgaccgc ttgttattta
     5401 ttgtattggt cgataacatt cgcctgcact tccgcccctt gctcgttaat cggctgccat
     5461 tgtgggtaaa cggcttggct atcttggctt gccataccta acttaacagc ttgatcgaca
     5521 ataatacgag ccacgtcaaa atggcgggat tccgggaatc gggcaagctg ttcacgcaag
     5581 ataacgccta aatctatcgg tttaccttca gcacggaacg gcgctaaatg agcttgcatt
     5641 gccgaaacaa tttgctcttg cacatctgaa agcgactcat actctaactc acttggcaac
     5701 tcgcccaccg attctgcctc atttaacatt gcttcatcat cacgcaagtc cagtaacgaa
     5761 tcggcttttg cagtataaag caaccaaggg ctgttgtaat aatcttgaat cgattggcgt
     5821 aaccgttgcc cgaatacacg gtttttgtcc atatcaatcg ctgtacgaat aaatttatgc
     5881 acatggcggt catagccgat ccaaagatcg atagcttgtt gtccccagct gataatacgg
     5941 tctaacttat tttgtagatt cacaatcagc tgatcgataa aatccaaatc attacgagcc
     6001 atcaggcaac tttgaatccg caataattga gcttgcagtt tatcaccagc ggcatttaag
     6061 gtatcttgca actcacgcaa attccccgag gtttcgtcta acaattgctc acaactcata
     6121 attgcttcgt gccagttttg gctaagcagt tcggctattc gttcacggat ttggtgctga
     6181 ttttcgtcca taatccgttg tgaaagatcg atgctgtcaa agatttccgc caccgaatat
     6241 ttcaatgggg caaacacatt atttcgccaa aaacgttcat cttgtgcctg ctctattccc
     6301 tcttccgctg ctactgaagc tcgctgaatt tcatctgcca caatcgaaag ttgaatcgat
     6361 aaacgcaggg tagaaaattc ccgctggcga acgtaataat ttgaaattcc cacccctaac
     6421 ggcgtaacac gataaatcgc caaaccttcc gtaaattcgc tactaaaacg gtttaacaac
     6481 cgttgtctga ctaaatcatt aatcgcatta ttggcacgct gggtaaaagt ggcatcagat
     6541 tgctcaaacg aatttgaaac ataacggaaa agatcaataa gatcactttc taacaattca
     6601 ccatctgtct gttcttggtt ataaatagag atagcaagta gaaacgctaa gcggtcggaa
     6661 gaaagcgaaa gtgaaaattc acgatctttc gtccaagaga tgagttctgg aatagtctgt
     6721 gccaattcgt tctgcatacg ggcttaatct ttaatttcta tacaaaaatg gtatttattg
     6781 taactgaatg taatcaaaat gggaatggat aaaacgcaga caagagaaat gaacaatata
     6841 agcggtcaaa tatgggtgat tttttgcaaa aagtatttta aaggta
//
LOCUS       DANLUA010000134         6641 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLUA010000134 DANLUA010000000
VERSION     DANLUA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1995
                     /locus_tag="PXB44_001952"
     CDS             <1..1995
                     /locus_tag="PXB44_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5282402.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAGSFGRGLMGAGVGIAAGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRI
                     DGKRELLDAVQKAVSVGGGSKEEALSSLDALLASGAVSTETALNLLPTLQKGAVATGA
                     SANDMAQIAISSMQQFGIKEEDIGRVLDMAVAAGQAGSFELADMASWLPQQMAAAKQA
                     GLSGIEGFERLLIANQQARVTAGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKT
                     GKTKAIDFAKSMEHYKGKGQDSLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEEQKRL
                     FNELTNLVEGTAIGEIISDRQALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIR
                     DTNAHKVESLKNTTEFAQMKNFEKVNGVLGTLAELLNKYANEYPNLTQFLSGATDAVK
                     IFGAALAASSVLDLLTGKKDKGGLIGDVLDKSSKASGGKGKVGKLLSVGGSFASVAGV
                     ATMLHGDQAPMSEERKAELRDPNYQARKMYEYKKEILGSRFHSFFRSNETLEQYRKDV
                     SAYEAKQKNSNTMGAVQGVLSTIPSLQELRPKVDSVTQTLSTYQADFQAFGATISAGI
                     EAGLASQSHTIANQITVELDGSVVAEKVAEYQFNFNKRMA"
     gene            1995..3287
                     /locus_tag="PXB44_001953"
     CDS             1995..3287
                     /locus_tag="PXB44_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDL5282403.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVVEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAFEDSLISKIDKLLLDLENLFDDGLRFWDSVG
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLRDGSGALNQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAIDNTQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELVEEREEELLIGDLELINQIVREHWLNIINKLREQQKNT
                     QKGVPSDKTKVIYETAEKLINNIRNVAHQFTALVLAVINKKPPLTVRMVMDSGTIHKV
                     AHAFYGDYERADELLRLNPHIRQPNFIERGTLLNCYWE"
     gene            3290..4417
                     /locus_tag="PXB44_001954"
     CDS             3290..4417
                     /locus_tag="PXB44_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282404.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            4419..5081
                     /locus_tag="PXB44_001955"
     CDS             4419..5081
                     /locus_tag="PXB44_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5282405.1"
                     /translation="MMRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSA
                     LADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLKNGE
                     TAIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQG
                     QISGNGGMVVQGGSGASFTGSLTQQGGSISTDGDVVASGKSLVTHQHNEQGDGRPTSA
                     PI"
     gene            5200..5550
                     /locus_tag="PXB44_001956"
     CDS             5200..5550
                     /locus_tag="PXB44_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL5282406.1"
                     /translation="MDREISPLTRDYTSENITTLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            5563..6624
                     /locus_tag="PXB44_001957"
     CDS             5563..6624
                     /locus_tag="PXB44_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5282407.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDAALLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
BASE COUNT         1955 a         1228 c         1680 g         1778 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctcgcaa
       61 aatgagctac gccgtgcctc agaagcgact aagcaacgag tccgagagtt aaatgctgaa
      121 ttgggtaaaa ccagttttgg ggataaggca ggaagttttg ggcgtgggtt gatgggtgca
      181 ggggttggaa ttgctgcagg tgcagcaatg gctcgccagc ctgcaagaaa tcaaatggaa
      241 tttgatagac agcttgcaat ggtagcgaat accgcctttt cagatcgtga tgtgaaaggg
      301 cgtattgatg ggaaaagaga gttacttgat gccgtacaga aagcagtaag tgtaggcggt
      361 ggctcaaaag aagaagcgtt gagctcacta gacgctttac ttgcctcagg tgctgtttct
      421 accgaaaccg ctctcaattt actcccaacg ttgcagaaag gtgcggtcgc aacaggggca
      481 agtgcaaatg atatggctca aattgctatt tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg gtcgagtctt agatatggca gttgccgctg ggcaagccgg tagctttgag
      601 cttgcagata tggcaagttg gttgcctcaa caaatggccg ctgcaaaaca agctggctta
      661 agcggcattg agggatttga gcgactttta attgcaaacc aacaggctcg tgtcactgcc
      721 ggtacaagta acgaggctgg taataattta gtgaatctgt tgggtaagat tactgctaaa
      781 gaaactaatg agcgttttaa gaatattgaa tataaagatc cgaaaactgg caaaaccaaa
      841 gcgattgatt ttgcaaaatc tatggagcat tacaagggaa aagggcaaga ttcattgcag
      901 gcttttatgt ctattatgga tgacgttgtc gggagtgata aacgctatca agaactacaa
      961 gctaaactta aaactgctaa aggcgaagag cagaaacgct tattcaatga attaacaaac
     1021 ttagttgaag gtacggcaat tggagaaatt atttctgatc gccaagcatt aatggctcta
     1081 ttagggataa agaataatgt tcaattaggt gaaaaagtac agaaagatat tcagaatagt
     1141 gaaggagctg ttgaaacctc tcacgctgtt attcgggata ctaatgctca taaagttgaa
     1201 tcattaaaaa atacaactga atttgcacaa atgaaaaact ttgaaaaagt aaatggagtg
     1261 ttaggcacat tagctgaatt actgaataaa tacgccaatg aataccctaa cttaactcaa
     1321 tttttaagtg gtgcaacaga tgccgttaaa atttttggag cagctcttgc tgcatcaagt
     1381 gtgcttgatt tattaaccgg taaaaaagac aagggcggac taattggtga tgttttggat
     1441 aaatcatcaa aagctagtgg aggtaaaggt aaggtaggta aattgctttc ggttggtggc
     1501 tcttttgcta gtgttgcagg cgttgccaca atgctacacg gcgaccaagc cccgatgagt
     1561 gaggagcgaa aagcagagtt aagagatcct aattatcaag ctcgtaaaat gtacgagtac
     1621 aaaaaagaga ttttaggtag ccgtttccac tcgttttttc gtagtaatga aacccttgag
     1681 caatatcgga aagatgtgag tgcttatgag gctaagcaaa agaatagcaa tactatggga
     1741 gctgtacaag gggttttaag taccattccg agcttacaag agttacgccc taaagttgat
     1801 tcggttactc aaaccctatc cacctatcaa gccgactttc aggcttttgg ggcaacgatt
     1861 tcagccggta ttgaggcagg gcttgcctcg caatctcata cgattgcaaa tcaaatcacc
     1921 gtggagctgg acggttcggt ggtggctgaa aaagtggctg aatatcagtt taattttaac
     1981 aagaggatgg cgtaatgagc ggttggacaa tgcctattca acaggcgagt tttaaaggtg
     2041 tgcgttttga cgtgcttgca gtggatgata gctttgagcg agcggtagtt gaacacgcct
     2101 acccttttgt aaatggggct gatttagagg atatggggct aaacacccaa actattcggc
     2161 tgcaagcggt cttttttggc tcaagctatt acacggacta tatccgctta ctttcagttt
     2221 tgcagcaaaa aggggctgat gtattagtcc accctattcg tgggcgttta ccgaatatgt
     2281 tactcgtttc ggcgaatcta cataccgatg ccgaaaatat caattatgtt gccttagatt
     2341 tgacctttaa agaggcaacc gaaatgcagc cgatttttgc ctttgaagat tcgctgattt
     2401 cgaaaatcga caagctgcta cttgatctcg aaaacctgtt tgatgatggc ttgcgttttt
     2461 gggatagcgt tggggtgttt gatctgaaat ctcgtttgct tgggctttgg ggggcgttgt
     2521 ttgcctcgtt tgaggcggtg cgtaacctgt ttgacttgga caagaaaaaa tacagcattg
     2581 cctcatcaac ctcacaacgc acctatttaa gagacggttc gggggcattg aatcagttaa
     2641 aagaaatggt ggatagcggt attcaggggc gtgctgattt aacgacactc tcgtttaaat
     2701 ctcagttact caatattact gatgcgatag ataacactca ggcgattgtg agcaaagtgg
     2761 ctagagatga gggttttaac cgctcaaaaa cggttaagtt acttggctct gatgtggttg
     2821 agcttggcat tatgcttgac cttattgcca cgactaagct caccaaagtc ttgactgagc
     2881 tggtagaaga gcgtgaggaa gagctgctaa ttggtgattt agagttaatt aaccaaattg
     2941 tgcgtgagca ttggctgaat attatcaaca aactgcgtga gcaacagaag aacactcaaa
     3001 aaggcgtgcc gagtgataag acaaaagtta tctacgagac cgcagaaaag ctgatcaaca
     3061 atatccgtaa tgttgcccat cagtttactg cacttgtgct tgcggtaatt aataaaaaac
     3121 cgcctttgac cgtgcgaatg gtaatggata gcggcacaat ccataaagta gctcacgcct
     3181 tttatgggga ttatgagcga gcggatgagc tattacgtct taatccgcat attcgccagc
     3241 caaactttat tgagcgaggc acgctgttaa attgttattg ggagtaaaaa tggaaaatga
     3301 aattgtggtg gagattgacg gctcgcagca taaaaactgg aaaagttatg atattgacag
     3361 cgatttttta attcctgctg atagttttaa ttttgagttg ggtaaatcta gcaatataga
     3421 ggttttacct aattattcgg gcaaaacggc aatagttaaa atcaatggca aaacggtgct
     3481 cactggcatt gtggatacca cacatcacac cattagcaaa agcgggcgtg ggtttagcct
     3541 aaatggacgt gataaagccg ctattttggt ggattgctct gccccgatta ccaatgtgca
     3601 agggctctct ttgcttgatg ctgttaaaaa aattgtaacc cctcttggga ttaaagaggt
     3661 gcaactgaaa gccgagaaaa acccgacttt aagcaaggtg gatattgatg tgggtgaatc
     3721 ggcgtgggag gcgattatgc ggtgtgccaa ctcggcaggt ttgcattgtt ggtttgaccc
     3781 caaaggcgtg ttaattgtcg gcggtgcgga ttacgctcaa ccgcccgttg ccacgttata
     3841 ttgcgtgaaa gagtcggggg atagccgtaa aaataacttt gttgatgcaa cactttctta
     3901 tgatgtgtcg caaagctatt cggaggtcac ttttcttgct caaaaacacg gcaaagattc
     3961 tgataatgcc aagcacgatt ttaaatgggt gtataaaaat gaggaaatgg agctttataa
     4021 accgaaaact atcgtgctgg gcgatgtgga gaacctcgaa gccttgatga aacaggcgaa
     4081 aaaacaggta tcagattggc agttggagtc atttaacctc acgattattg tacctgacca
     4141 taaaaccaaa gacggcacgt tgtggcaagc tgggcaacgt gtgcacgtgg tatgtgagga
     4201 gtatgattta gatggtattt tctttttaat ggggcgacgt tttcgactct ctcgcacggg
     4261 tggcacaacc accgaattgc ggttaaaact ggatggcgtg tggacacctg atgcttatca
     4321 agctaaagca caagaggctc gcaagcgtaa aggcaaaaaa ggacgtaaga aaaaagagcc
     4381 tgtagaatat gtaggctcgt gggagttggg aaaataatat gatgagaaaa ttagcaaaac
     4441 agactaaaga ggtggcaaaa ggtgtgcaag atagcgtgcg tgctgctttt agaggggtat
     4501 taaatttagt caagagtggc agtgatattc agcaggtaca ggtgtccgct ttagcggacg
     4561 aaactatcca agatttagag ctaatgcagc attttggttt cacttccgtg ccacctgccg
     4621 gcacgcaagc ggttgttatc cccttaggtg gtaaaaccac gcacggtatt attgtggcga
     4681 ctgaaaacgg ctcgtttagg gttaaaaatc taaaaaatgg tgagacggct atttatgatt
     4741 caagcggctc aactatcata ttaaaaaacg ggcgagtgat tgatattgag tgcgatagct
     4801 ttaacattaa atgcaagcaa tattccgtca ccgcaagcgg tggagcaaat tttaacaccc
     4861 cgaaactaga aaccgaccag cagtttgtgt ctcaagggca gatcagcggc aacggtggaa
     4921 tggtagttca aggcggaagt ggtgcaagtt tcactggctc attaacacaa caaggaggca
     4981 gtatctcaac tgatggtgat gtggtggcat cgggtaaatc actggttaca catcaacaca
     5041 acgaacaagg cgatggcaga cctacctctg ctccaattta acttacaagc taatttacaa
     5101 gcggtctaat tttgcaaatt ttttgtagaa aaagaccgct tgccatttgt ggaagtctct
     5161 ccccgttact cctgtcttta atgtctctac catagcaata tggacagaga aatcagcccg
     5221 ctcactcggg actatacaag tgaaaatata actacactgc aaaatgccgt gtatatcaga
     5281 ctaactactc cgaaaggctc gtggtgggca gatgggcgtg taggttctct gctccatctt
     5341 attcaacgtg agaaagattt aagccgtgtg gggctattag ctcaacagta cgccgaagag
     5401 gctttgcagc cgattattga tgacggacga gccaaaagca ttacggtgac acacgagcag
     5461 ccaaaagacg gctcgttaaa tcttttaatc tccgttattg ataatcgtgg ggttaaattt
     5521 gagtttaaac accctgtaaa actggtttag gtggttttta aaatgtatca aatcccaacg
     5581 cttgaagatg ttagaagaga aattctgcga gatattcaat ctttagagcc aactgctgat
     5641 attgaggtgg attcggatta ttttgttcgt gcctccgctt tggcaagctg tgtggtgggg
     5701 ctgtatgctc accaaaactg gattgttcgt cagtatttcc cagatacggc agactccgaa
     5761 tacctcgaaa tgcacgctaa attgcgaaat ctttaccgta aaaatgcgac ctatgcgagt
     5821 ggttcgttga aagtttttgg tactgctggc tcagtaatca atgagggctt gcaagttaaa
     5881 tttggcgagc gtttttatgt aaccaaaagc tcggacgtta ttggtgatga tggctctgca
     5941 acggtgcgag tgatttcaat ggcaacaggg gcatcaagca atgttaaatc tgaaagtaaa
     6001 gcacaactga ctgctgctcc tgctggcgta tcaagcgatt gcttgttatt gcctgatgtg
     6061 acaggcggga cggaagtgga aagcgatgcg gcattgctgg ctcggttatt agagcagatt
     6121 cgccgaccac cgtcaggcgg gaataaaaat gacttccgcc aatgggcaga aagcgttgat
     6181 ggcgtaacct ctgcgtttgt ttatccatta cggcgtggcg agggtacggt agatattgca
     6241 atcacctcag gtgataccgt gccaagtgat gaaatcgtgc gagcaactca agcttatatt
     6301 gatgaagtcc gccctgttac ggctaaatcg gtttatgtac ttaagccagt tgaaaaacgg
     6361 gtcaatttta acatcaaagt gcaacttgat acggatacga cgcttgatgg tgtaaaagcg
     6421 gaaatcgaaa acgctttaga gaattatttt ttatcactca aacctgctga caccttaatt
     6481 atttcgcaaa ttgaggcagt aattagtgat ttagtgggcg tagtagatcg tgagattgta
     6541 cagccaacca gaaaccaaac gattgatgcc taccgtgaaa tgggctggtt cagattgggt
     6601 gatgtaactg tggagttgat gtgatggtaa cgattaactc t
//
LOCUS       DANLUA010000135         6637 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLUA010000135 DANLUA010000000
VERSION     DANLUA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1962)
                     /locus_tag="PXB44_001958"
     CDS             complement(<1..1962)
                     /locus_tag="PXB44_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL5282408.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLVLQNLNNK"
     gene            complement(2040..2522)
                     /gene="ybeY"
                     /locus_tag="PXB44_001959"
     CDS             complement(2040..2522)
                     /gene="ybeY"
                     /locus_tag="PXB44_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL5282409.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            complement(2555..3010)
                     /gene="moaE"
                     /locus_tag="PXB44_001960"
     CDS             complement(2555..3010)
                     /gene="moaE"
                     /locus_tag="PXB44_001960"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL5282410.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            complement(3012..3257)
                     /gene="moaD"
                     /locus_tag="PXB44_001961"
     CDS             complement(3012..3257)
                     /gene="moaD"
                     /locus_tag="PXB44_001961"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL5282411.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            complement(3250..3726)
                     /gene="moaC"
                     /locus_tag="PXB44_001962"
     CDS             complement(3250..3726)
                     /gene="moaC"
                     /locus_tag="PXB44_001962"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL5282412.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            complement(4227..5252)
                     /gene="moaA"
                     /locus_tag="PXB44_001963"
     CDS             complement(4227..5252)
                     /gene="moaA"
                     /locus_tag="PXB44_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL5282413.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     regulatory      complement(5241..5397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5561..>6637
                     /locus_tag="PXB44_001964"
     CDS             5561..>6637
                     /locus_tag="PXB44_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282414.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQK"
BASE COUNT         1856 a         1398 c         1366 g         2017 t
ORIGIN      
        1 tttattgttt aagttttgca aaacgagttt tcgataattt tccacctcca ttttggagat
       61 tttttgccct tttcgttcaa aaatatccag ctctgaaaag cgtgctttat ccgataaata
      121 aagccgtttt aaactcacat acgccgctga aaacgtccgc ttgccatgag caaacgcggt
      181 taaatttttc caatcacgtt catctaaatt gtgtgtggca agcggtcgaa attgttcgaa
      241 attttgcaat tcctcacaaa tttccgtgaa aaatggctga agggcgaaat ctctggcaaa
      301 ggcttgggtt gcctgctgtg caaataactt tccttgctcc gaaatcgggt aaatcatcat
      361 tgcactttga taaccactgc ttgcctcttt attcggtgtg atttgcacaa gttggaaacc
      421 acaagattgc caaaatgcgt ggagtggagt agttagccca aagctcacag agataaaatc
      481 aagcggtcgt ttttctaccg aattttgcaa aataaactga gaaatcaacc gcttgccaac
      541 gccttgattt tgcttttcag gcaataccgc aatccgagaa atgcgaatgg aatgcaattt
      601 gcaagcctgc ggtaaattcc cttgaaaaca gagatattga gccactagac tgccttgcgg
      661 acggcgttcc cctagccaaa tggcttgggt taatttttca tctaaaccac cctcttgcat
      721 tgcccaaatt ccgccgataa gttgctcgtt ttcctgttgt gaaaacaaaa gctgattttc
      781 gccatcaaat aaacggcgta agtcagttgg agtggttttg taatgggcgt tggcgagtaa
      841 gtggtagaaa gcgatttttt cctgtaaatg cttattcgaa taacttatat tcttcgataa
      901 ttgcttctgc cacaaacgct ttgcgtttga acgttgcttt agcaacggca catagtgtga
      961 gcgaagcgaa taaatctccc ccttcccctc tttgctaaag aggggagatt tttcttcgga
     1021 aagtaatagc aactcagtca caaatgcttc gagaggatca ttttcgctcc accgcaatgg
     1081 ttgagataac gtccaatgtt taaacggttt atccaacatt accgggaatt tcagctcaaa
     1141 tcctcgcccc gtgccttcgt aattttgggt tgttgtggtt aaaaccactt tttcaaaata
     1201 ggcacaaagt gtgtggagta tcggcaaggg taggctagct gcctcatcaa taaagagcca
     1261 ttggttaggt gaaatgtttt tacttttaac ttgctcgatt aaattgtcag gtgcgaaaaa
     1321 agggattttt tgctctgccg aacgccaaaa actgggcaat gcagaataag accgtgcggt
     1381 aattaatacc ttattttgtg cgggtaacgc ttttgcaagt cgccctgcaa gggtagattt
     1441 gccccgccca cgaggggcgg taatcaaatg aatatcggca ggatcaagcg gtagattttg
     1501 caaaatattt tgctgttctt gcgttaattg gtaaattttt gaaggaattt gaccgcttgt
     1561 agccggtaat tttggcaaat tagttctgac ttcaaaacca aatttagcga ctaattgttt
     1621 aaaatgttga tagaaattcg ggcaggcaat agcctgattt tcgttccaac gtaacgcatc
     1681 gaaatctaat tcattttcca aattatccca ctgaggacaa agcaaaaata aagtaccgtt
     1741 ttcttgaata gtgccggcaa gtattgcaaa agcatctaag ttaaaattca ccccattttc
     1801 tgcttgcata gtataaatgg caaaagggta ttcttgtcct aaaatcgttt tggcattgga
     1861 aaaggggata gctttaagct gatgattttc gggaatgtaa aaatagcctt gttgaataaa
     1921 gtcggcattg ttaggagatg ttatgattgc gatcgttctc atataacttg taggaacagc
     1981 tcctgtgcct gtccgattat aatggagtac tggagggcac aggaccctcc cctacaagac
     2041 tatatttttt ctgaaagata cggatcttca taccctaaac tttgcataat ttcggtttcg
     2101 aggctttcca tttccactgc ttcatcttcc tcaatatggt cgtagccgag cagatgaagt
     2161 gtgccgtgaa tcgccaaatg agcccaatgg gattcggctg aaataccttg ttcttccgcc
     2221 tctttttcca ttacttgacg acagatgact aaatcaccca atagtggcat ttcgattcct
     2281 tcgggggcct caaacgggaa agatagcaca ttggttggct tgtctttacc acgataagtc
     2341 agatttagct cgtggctttc tgcttcatcg acaatccgaa tggtgatctc actctcaggg
     2401 aaatcatcgg tttttgcctc aactgcgagg gctttttgta cccaagtata aaattgttgt
     2461 tcacttggta agttttcggt attttcgcag gcgatctgca aatcaatata taaattttcc
     2521 attcgttacc ttttgcaaat tattgccatt ttttctattg ccattttttg gcggcttgtt
     2581 ggtcactctc acgcccttca atccaacgtt caccggcttg ggtttgttct cgtttccaaa
     2641 atggggcttt ggttttgaga taatccataa taaactcgtt ggcgtggtag gcatcacctc
     2701 gatgagcaga actcacaccg actaatacga tttcatcacc ggtatgcagt tgcccaatgc
     2761 ggtgaatcac cgcaacgcgt tgtaaatccc aacgagtgcg agcttgagcc acaatttctt
     2821 gcaatgcttt tttagtcatt gccggatagt gttcaagaaa caaaccggaa acattatcgc
     2881 ccaaattcat ttcacgtact ttaccgatga atagagtggc tgcccccacg ctatgttgtt
     2941 cactgagcca ttggtaaatg cggttttggt caaattcagc ttcttgtact tcaatcaggg
     3001 tttcaaacat tttagccccc ggttaccggt gggaagaatg ccacttcatc gccgtctttg
     3061 atctccgttt caagcgggca gatcgtttga ttaattgcaa ccagcagttt gcctttttca
     3121 agggcaagtt gccatttttt accctgttgg cttaagtgtt cacgcaacgc ttcggcagtg
     3181 gtaaaagtag gctcaacatc tagtctatcc gttgccacaa gttcacgcac ttgggcgaaa
     3241 aaaagtatct taatcaacct tgaaatctcc tgattttccg ccacttttac taagtaatct
     3301 tacattttca ataatcatat ctttttgcac ggctttacac atatcgtaaa tagtcagagc
     3361 agcaacacta gctgcggtta atgcctccat ttccacgccg gttttgccgg ttagtttaca
     3421 gagtgattca attcgcactt ggttgctttc cggcaacgcc tccagttgaa cttccacttt
     3481 agaaagcaat aacgggtggc agagtggaat cagctcccaa gttctcttcg ccgcctgaat
     3541 accggcaatt cgagcggttg cgaatacatc gcctttgtgg tgattaccac tgacgatcat
     3601 ttgtaatgtt tcggcattca ttcgcacaaa tgcctccgct cgggcaatgc gtacggtttc
     3661 ttgtttcatt gaaacatcaa ccatattggc ttcgccgttt tggttgatgt gggtgaaggt
     3721 tgtcatttgt ttgtctcttt aaatttgatt gtaaaattaa tattgagcgt cttcttgtta
     3781 ctttctttgc ttgtgcaaag aaagtaacca aagaaagcac accctacttc accgctatct
     3841 tcattttatt tgaatttgtt taacggaaaa atttaaaact cgccaacttc gttggctcaa
     3901 acactaaatt tttcctataa attcaaagtc gctcaggcgg ttcagaaggg agaagctgga
     3961 acattacaag cggttatttt taaataaaaa cttgcaaaaa tcttaaataa tgtaccgctt
     4021 gcccctattt tccgccgtag cgattgcgta aacttttgag gacaactttt cgtgtttgag
     4081 ccaacgcagt tggcgagttt gaaaagttcg tcccaaaagt tagcaacaag cgaaggatta
     4141 aggaaaatct ggggtcgcct ttcttttggt tacttttctt tggcgaagca aagaaaagta
     4201 acagaaaaaa cacgctcggt ggttgtttaa ccaccgatac tcgccaaatt attcctaacc
     4261 ccactatccc caatatgcaa atagtggtgt tctcgcttgc cttgtaagga agattttagg
     4321 cgggtttcaa gctgtaactg ttgctcatcg cttgccagta aatcacgaat atcaatccct
     4381 tcttcaccaa ataagcaaag atgcagttta ccaagtgcgg aaacacgcaa gcggttgcaa
     4441 gaggcacaga aatttttctc gtagggcata attaagccga tttcgccttt aaaatcaggg
     4501 tgagaaagca cttttgccgg gccgtcagac atcgctttgg gttgtaattg ccagccttct
     4561 tgtaataagc gggtcatcac cgattgacca gaaagatgtt gagcttggaa gaaactatcc
     4621 atttcaccgg tttccatcag ctcaataaac cgcatttgaa tcggcttgtc tttgatccaa
     4681 gtgaggaatt tatccaattc aggggcggta tattgcttca ttagcaccgc attgacttta
     4741 atttttgggt agccaatttc aaaggcttta tcaataccct ttaaaataga ttgcaacttg
     4801 ttctcgccgg taatcagttg aaattggcgt gggtcaaggc tatcaacact cacattaata
     4861 tcggtaatac cggcttgttg ccacagttca acatcacgct ctaggcgata gccgttggtg
     4921 gttagggcaa ctttacggat accgggtgtt tgtgaaatag tgtgagcgat ttcgaggaaa
     4981 tctttgcgta aagttggctc accaccggtg atacggattt tttccgtccc taaattggca
     5041 aaggctttag ccacccgctg gatttcatca acgctaagaa aagtttgctt atgtgaaggc
     5101 ggcttatagc catcaggcaa gcaataatta caacggaagt tacaaacatc agtaatggat
     5161 agtcgcaaat aagtatattg acgctgaaag cgatcaacta aacctgaaac gacatcatca
     5221 cccacgtttt taattgggat aatagactgc atttgacacc tttctaaaca cgagaggaca
     5281 acccatttct aagttatcct ttgaacagct ttatgctgtt actggcataa attccatatc
     5341 aaaaatttgt ctttaatttt tagagttagg aaataatgct cggagtcttc aataaattgg
     5401 ttttcatttt atggtgagaa acctaattag gcaagaggta taatttaaat tgtatgctct
     5461 acctataaaa atctggcaac cgttgcttag tttgatttat atcatattaa tgccgagata
     5521 attttgctat tttagctaac gtttttagtt cgaggtgaga atggataaac gtagccgatg
     5581 gtcttgttta gtaaatgaac aaggcgaaat atcagaatgg gaagacaaag caggggcaga
     5641 ctattttgct gaattaccgc aaatttgcca ctatgcacag gcattgctct attcagataa
     5701 aaatagcttg cagttttact atagttcaaa ccatcaatgg cttaagatca gcctagaacg
     5761 ccaccaatcg tttgtattga ctcaagcaga gcataccgat ttgccttatg gactgagtaa
     5821 aagggagatt gagcttctca ctctaattag cagtggtttg agcaatgagg atatcgccaa
     5881 tgagctttgt atcagcaaac ggactgttga taaacatatc gaaaatattt ttgcgaaaac
     5941 acataccaaa agccgaacct tattagctgt ttttgccatt accgaacatt taatctgttt
     6001 acccacaccg ggaaaactaa cccaatccat tcttgccagc tttaacattg aacaactggc
     6061 attgcaaatt atcgcgaaaa aacaaccgct tgtaacggct tcaactcgac acgaaccacc
     6121 gatttttacg ataaaccacc atcatagtcg tccgattatt attggcgtgc cttatgttga
     6181 acagggtatt gggcaaatcg atacccgaga gctacttaac ggcactcaac ttgcggttga
     6241 gaccatcaat caacaaggtg gtattcacgg cagagaagta caggttgcaa cggctgggtt
     6301 ttatgttaaa gataaagcaa gtattcttca ggcataccat catttatttg atcaagaagt
     6361 tgatgcgatt tccaccgctt atgcctgcta cttgccggaa gtacacgaat taattgcaac
     6421 taaaggcatt ccttatttgc atattgcaac ccatagtggc tcaaataaac ttgcccaaaa
     6481 tttaccgaat caacgaattg aaaatatgtt tcaggtttgc gccagtgatg taacctatgg
     6541 cttaggtata tctcactttc tgcaatatta ccgttaccat tatgcccctt ggcttcagaa
     6601 taaaacggtg atggtggtaa cggtgagttg gcaaaaa
//
LOCUS       DANLUA010000136         6590 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLUA010000136 DANLUA010000000
VERSION     DANLUA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(59..319)
                     /locus_tag="PXB44_001965"
     CDS             complement(59..319)
                     /locus_tag="PXB44_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282415.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILFPYEY"
     gene            complement(432..794)
                     /locus_tag="PXB44_001966"
     CDS             complement(432..794)
                     /locus_tag="PXB44_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282416.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            complement(885..1235)
                     /locus_tag="PXB44_001967"
     CDS             complement(885..1235)
                     /locus_tag="PXB44_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282417.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            complement(1333..1740)
                     /locus_tag="PXB44_001968"
     CDS             complement(1333..1740)
                     /locus_tag="PXB44_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5282418.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            complement(1764..2411)
                     /locus_tag="PXB44_001969"
     CDS             complement(1764..2411)
                     /locus_tag="PXB44_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL5282419.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            complement(2486..3474)
                     /locus_tag="PXB44_001970"
                     /pseudo
     CDS             complement(2486..3474)
                     /locus_tag="PXB44_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            3640..4743
                     /gene="proB"
                     /locus_tag="PXB44_001971"
     CDS             3640..4743
                     /gene="proB"
                     /locus_tag="PXB44_001971"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5282420.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            4767..5090
                     /locus_tag="PXB44_001972"
     CDS             4767..5090
                     /locus_tag="PXB44_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282421.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(5150..5704)
                     /locus_tag="PXB44_001973"
     CDS             complement(5150..5704)
                     /locus_tag="PXB44_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5282422.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(5729..6589)
                     /gene="psd"
                     /locus_tag="PXB44_001974"
     CDS             complement(5729..6589)
                     /gene="psd"
                     /locus_tag="PXB44_001974"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658144.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5282423.1"
                     /translation="MSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAVTHFVIKLFA
                     KKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADGRISQLGHIE
                     QDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMPCDATLCKMI
                     YVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITASMSTVWAGV
                     INPPRPEKITTWAYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLASHLQVDSPV
                     RMGEILAHQK"
BASE COUNT         1950 a         1372 c         1353 g         1915 t
ORIGIN      
        1 cgccatcatt tgacggcgtt tgataacata aaaatgtagg gtataaatgg aagctccatt
       61 aatattcata aggaaataaa attgtagtgt aactacgatc agcctctgtg attacataaa
      121 gtgtttcatc ttccacatca tagcgagcta aaatgcgtcc tccgcgttca atagctttca
      181 cattggcttt cttgtcgtta tcacacaaat ttccccaatc accactgcag tagcgatgaa
      241 gtagatcaac taatgtatgt tgattgaaac attcagcaac tgcacgagtt gcacatactc
      301 taccaagctc aaaagtcata catttctcct aaacagaaag aggggaaatg tcatcccatt
      361 ggggagatat ttccccaagt gggtgaatta agacgacagt aagcgtcatt tttagtaaaa
      421 atttgcaaaa attaggcaac taatttagcc ggcgtcattt cttcacgaat cttatctaac
      481 atttcattcc aatcatctat atcacagatt ctttggctat taatatagaa atgcgtatct
      541 attgcattga tataataatc aaattcgact aaacctgaat cttgctcaat attcagctca
      601 atacagaatc gagcaatcaa cttaaggatg acacgctcct ggtataggtt gccaaaatga
      661 ggtacggtat caaattcttt agcggaatgc caaaaatctt caactgaaag acaatcatca
      721 tattctttca gtatatcctc ccagttacct aaatagcatg aggtgtcgtc aaggtcaaaa
      781 aaggtgtcta tcattatgtt tctccaaatt caaaataaaa gtgggaaaca atctgcccta
      841 tcggaaaaat tgtttcccga cagggtggat agttctgata ccaatcaaaa cacttcatag
      901 tgagctaatg ataaataagg gcaaacattt tcatttgcct ccatctcatc atttagcttg
      961 cacataaagc gatataaggt atcaaaaaca gcatccatat tatgaacagt aacgtataat
     1021 ccgttttgtc tgagtttata accacataat tttgctatca attcatcaag gctaatcaat
     1081 tgattatcct taatgacaca tacagcaatt tgccgagagg tattatttct cgacacattc
     1141 gatacactac agtgaaaaaa ccaaggtgta ttacggttat tccagacaaa ttcaactaac
     1201 tcacgttctt gttgtgaaag gttaatagtg gtcatcatgt ttctccaaat tcaaaataaa
     1261 aatgggaaac aatctgccca ccttgggaaa attgcttccc aatagggtgg taaataataa
     1321 gatgagtaaa tatcagcgat aaatatcacc ggagacggtt tccaccgaac cggtaatagc
     1381 acgacagcga acatcaccac tcacggtttt cacataagag gcaacatttc ccttaataga
     1441 gacatcaccg ctaactgtgt caacactgcc ggcatctcct gtaatagaga catctgcggc
     1501 accggcactc agtttctgca cattgcccaa aacctcaatt ttgaggactg gctcagaacc
     1561 taattcaatc gactgtccat tcaccaataa ttgattacta ttggacgaga ctaaaatact
     1621 tcctttgttt atgcctacga ccgtttgaat attgcagcca aaaaatccaa tgctttgaat
     1681 atcttcgcct tgtgaatcaa acacctgttt gccattaata taaatacctt gtcgattcat
     1741 atcgtcatct cctttgtaaa taattatgcc caaagtaatt cagctaattt tacttttttc
     1801 tcaagttcat taactgcttt tttagcataa gtcagtgaat aaccatgctc tcgtttctcg
     1861 gggttgtcct tcaattcttt gtgctttgtt ttggctttat ccaattcaaa cttaaagtat
     1921 tccagacttt ccggcataga caggtcaatt tttcctgcca ttttttccca atatgcgata
     1981 cgagattcat aacttgcagc tttgttcatt tctgcaaccg cattgtccat acggcgagca
     2041 ttacgttcaa taaggttacg gtgtctcttt tcgctgtgat gaccaatttt tattggctca
     2101 cctaaactca aaaaatcaca cccctcatta gcggcattta cccaatcttg actacgtttg
     2161 gctgcattat ctgcatagcc ttgatagcgt tctgcttttt gttgagcaaa tgactgacta
     2221 tccatcccat cacaacgagt aaatgaataa aaataatgct cgccatcttg tttaacaaga
     2281 ttatgtactt gaacctcggt ttcattacca tatttgctgg ttagcgtaat aatttcacct
     2341 ttggtatgtg attcagagca ttttgcaaca aaaacggtag gtgcaaattt tgcataagtg
     2401 ttaatagtca tagcgtttct cctataaagt aggggaaacg ccatcgagga gtttccccgg
     2461 tgggtaaatt gttgagataa gtaaattatt ggccttttga tgccataaat gcaatatagc
     2521 ctaaaccatt ttgcataggg tcttcgttat atttcagtgc atacagaatt ttcaaaatcc
     2581 gctcactttc ttgaatcccc attctggcaa cttcatccgt ttcttcactt tgataccaag
     2641 ctgcataacg ttgatctagc cgtagaaatt catcttgaat gaatttattt agcatacaga
     2701 tttcctctaa gaataaactc agcgcaaata caatgcctaa gcatgcccca aatgtgagga
     2761 ttgtcgcgag tgcccaacgg gcaatcccca tattgatatg cccttttaaa atatcggcga
     2821 gtgcattaat cagtggaaag ccgggaacga gcaataatac gctggatgcc aaggcaattt
     2881 ggggctgatt gccgatgtta tatttcaacg cgatacccgc aattagtgag gcgacaaagg
     2941 cggtcaccgc aaatacaatt aaggggttat agtgtcttgc ggcaagtgat tggcgaacat
     3001 acatggcgag tgttgcggcg agaaatgtaa ccaatgaaac tagtgcgtcc ccaccagata
     3061 aatgagcaaa actggcacag gatacgccaa tcatcaacat cacaaaatag cgattgtatt
     3121 taagcggttt aatgcgagcc agtttggcat gcacttgttg catatcatag attttacgtt
     3181 ctgctgtaat cacaatacgt tgcacttcgg taaccacttg catattaatt cctttgtctt
     3241 ggtttttacg gactgtcgtg atgcaatggt cttgggctaa tgtagtaaga ataatggcat
     3301 ttggcgtgag ggcacattct acgctatcta caccgagggc taagcctaag cgatgtgcca
     3361 tttgcacaac gatggcactt tctgcaccat gttgtataag agttgggcaa cttgaacaca
     3421 aaggcgagtg atttctcgtt gccgctccgt ttcttgtgaa gaggttgagg tcataatcgg
     3481 gttcctagct gaaaaatcaa gaagctttat tatatgcctt ttagtgagaa aaaatttgtg
     3541 ctaaatcata gaaaatagca agtaaacgag atgatttttc tgatgattaa tggtaagtta
     3601 gcttcaattt tgtgcgacaa aaataagaag atagagaaga tgaaaatcga caaaacactc
     3661 gttgtaaaat ttggaaccag tacgcttacg caaggatcgg cgaagttaaa tttacctcat
     3721 atgatagata ttgtgcgtca gctcgcacag cttcatcaag cgggctttcg tttagtgatt
     3781 gttacctcag gggcgattgc ggcaggtcgt cattatttaa atcacccgca attgccaccg
     3841 acgattgcct cgaaacaatt attggctgcg gtaggacaaa gtcagctcat tcaaacgtgg
     3901 gaaaaattgt ttgccattta tgatattcat attgggcaga ttttattgac ccgtgccgac
     3961 attgaagatc gtgaacgttt tttaaatgcc agagatactt tgcgcgcctt attagacaat
     4021 cagattattc cagtgattaa tgaaaatgac gctgtcgcta cgtcagaaat taaagtaggc
     4081 gataacgata atttatccgc gttagtggca attttggtgc aagcagaaca gttgtatttg
     4141 cttactgatc agcaaggttt atttgatcgt gatccgcgca aacatccaga cgccaaattg
     4201 atcgccgacg tggacaaaat taccgatcat attcgcgcga ttgcgggcgg aagtggcacc
     4261 tcgttaggca caggtggcat gtccaccaaa atcagtgccg cggatgtggc aacccgctcc
     4321 ggcattgaaa ccattattgc accgggtaat cgcccgaatg tgattgtgga cttagcttat
     4381 gggcaagcca ttggcaccaa atttagtgtg caagcagatc gcttagaaag tcgtaaacaa
     4441 tggctatatg cggcgccgtc agccggtatt attaccattg atgcgggggc ggaaaacgcg
     4501 atgttaatac aacataaatc gttattgcct gctgggattg taaacgtgga agggcgcttt
     4561 tcacgcggtg aagtggtaaa aattcgtact cagcaaggca aggatgttgc cttagggatg
     4621 ccacgttata acagcgatgc gctccattta attcaaggca aacattcgca agatatcgaa
     4681 caggttctcg gttatgaata tggggcggtt gcggtacacc gagacgatat gattgtgtta
     4741 taaatcagaa aaagaaaggt aacaagatga attggaaagc agtattgata ttcagtgtac
     4801 taggttcgct ggtgagctgt gcttcatact ctgaacatgc ggtgcaacgt attgaggcag
     4861 gcaaatcttt tgcagtgacg gggaatacaa aacggattaa taccatggca tgtcaagata
     4921 atgatgattg gtatttggac ggctatcgtg tgggaaaatc atttcgagaa caccaacaaa
     4981 aaatgttgat tcaacgaacg gcttactgtg aagaacaaac gggcaaggct gtgccggaca
     5041 agttccgcca ttcgtggaac agcggctatc agcaaggctt gaaacgataa acgctagaca
     5101 taaaaaagtg cggaaaatga ccgcactttt ttatgtctag cgtttagctt taaagaatgg
     5161 ctaataattc gacttcaaac actaaggtac tgaatggcgg aatggaggca ccagcaccac
     5221 gttcgccata agcgaggttg tgaggaatgg ttaaacgcca ttttgaaccc actggcatca
     5281 tggacaaggc ttcaatccaa ccagcgatca cgccatttac tgggaattct gcaggttgac
     5341 cacgttttac tgagctgtca aagacggtac cgtcgattaa tgtcccagtg taatgcacac
     5401 gcactttgtc ttcgcgtgcc ggaatttgcc cttcaccagc cactaacact tcatactgta
     5461 aaccagattc cgtagtattt acccccgctt tttgcgcgtt ttcagctaag aattgtgcac
     5521 caacggcttc aatttctttg aatttcgctt gttgttgcaa agtctgtagg gcatgactca
     5581 cttcatttaa ctcaagtgct ggtggatttt gatttaacac atcaaagatc cctttcgcga
     5641 cggcttcggc gctcacttca agttggcttt ctaatgacac ttggtcgaaa aattgttgac
     5701 tcattgtttt tcctattgtg gttaaaaatt atttttggtg ggcaagaatt tcacccatac
     5761 gtaccgggct atccacttgt aaatgagatg ccagttgcac acgatctttt tcaaataaat
     5821 tgatcaccgt cgagcccaat tgaaaggctc ccatttcttg tcctttcagt aatttgaccg
     5881 cactttctcc ttcataggcc caggtggtga ttttttctgg acgaggcggg ttgatcacac
     5941 ctgcccacac ggtgctcata ctcgcggtaa tggttgcgcc gactaaaatt tggaccattt
     6001 taccaaattc ggtatcgaat acgcagatca cgcgttcatt acgtgcaaat aagtttggca
     6061 cgtgttcggc taaaaatgga ttgactgaga ataagtcacc tggcacataa atcattttgc
     6121 ataaggtggc atcacaaggc atatgcacac ggtggtaatc gcgtggtgag aggtaggtgg
     6181 tggcaaattc gccatcttta aaatgatgag cgagcgcctc gtcgccagct aacaaatcgt
     6241 tcaagctgaa ataatgtcct ttggcttgta gcaataaatc ctgctcaatg tgtcctaact
     6301 gactaatacg accatctgcc ggtaaacaaa gtgcggtagg attttgatta attggacggg
     6361 cattgtccgc taatggacgg ataaaaaatt cattaaagct cgcatagtcg ctgaaatttg
     6421 gttttgcagc ttcactcata tccacatggt attttttcgc gaataattta atcacaaaat
     6481 gtgtgacagc accccattgc tgttttgcaa accagcctgc aagacgtgtt aaataaagtt
     6541 gtggcatcac atattgaaag gcaattttaa tccgttgcca gtaactcagt
//
LOCUS       DANLUA010000137         6559 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLUA010000137 DANLUA010000000
VERSION     DANLUA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            151..1074
                     /gene="oppB"
                     /locus_tag="PXB44_001975"
     CDS             151..1074
                     /gene="oppB"
                     /locus_tag="PXB44_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL5282424.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            1089..1988
                     /gene="oppC"
                     /locus_tag="PXB44_001976"
     CDS             1089..1988
                     /gene="oppC"
                     /locus_tag="PXB44_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL5282425.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            1998..3593
                     /locus_tag="PXB44_001977"
     CDS             1998..3593
                     /locus_tag="PXB44_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282426.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            3773..4168
                     /locus_tag="PXB44_001978"
     CDS             3773..4168
                     /locus_tag="PXB44_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5282427.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(4378..6537)
                     /locus_tag="PXB44_001979"
     CDS             complement(4378..6537)
                     /locus_tag="PXB44_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5282428.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARLSSQKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
BASE COUNT         1898 a         1298 c         1293 g         2070 t
ORIGIN      
        1 tgccgatttt tttacaaaaa taagctaaaa aaacaaccgc ttaagaataa tgaattctta
       61 taaaaatagc cataactaat caatatcttt acaaagccgt taatttacgc tattaattgc
      121 ctaccaatat aactaaaaag agtggtgctt atgctgaagt ttatttttaa acgtcttctt
      181 gaggcgattc caaccctact aattctgatt accttttcct ttttcctgat gcggcttgcc
      241 ccgggtagcc catttaccag tgagaaagcc tatccgccga aagtaatggc aaatatcgag
      301 gcaaaatatc acctgaatga accgctgtat aagcagtatt ttctttatat ggaaaatctg
      361 tcaaaaggcg atttcggtcc atcttttaaa tataaagatc aatcggtaaa taatctgatt
      421 gcttctgcgt ttcctgtttc gataaaactg ggcattactg cctttatttt tgcggtgctt
      481 atcggtttaa ccgcaggaac tatcgctgca cttaagcaaa atagtaaatg ggattattta
      541 gtgatgggct tttcaatgac gggtattatt atgccgagct ttgtctttgc tccgctgttg
      601 gtactcatgt ttgcgattta tctcaaatgg ttgcctgccg gtggttggaa cggtggtcag
      661 ctctactata tggttttacc tgttctctct ttaaccatta gctatgttgc cggcattgcc
      721 cgtattactc gtggttcaat gattgaggtg ttgcactcta attttattcg caccgctaag
      781 gcaaaaggct tgccaatgcg taaaattatt ttcaaacacg ctttgcgtcc ggcattgttg
      841 cctgtgatca cttatttagg cccggctttt gttggcatta ttaccggctc gatggtaatt
      901 gaaagcgtgt ttggcttacc gggaatcggg cagttgttcg taaacggagc attaaatcgt
      961 gactattcat tagtattaag cctaaccatt ttggtaggta cgctcaccat tttatttaat
     1021 gcgattgttg atattttata tgcggtcatc gacccgaaaa ttcgttattc ataggagtaa
     1081 agcggaaaat gcaaaattct tcaaaaaatc aaccgcttgt tgaggttacc tcaaccattc
     1141 aaggacgaag tttatggcaa gatgcacgcc gccgtttttt ccgaaataag gcggctgtgg
     1201 caagtttgat tattctttgt tgtattattc tttttattac ctttgcccca atgctaatgc
     1261 cgtttagcta tgaagatacc gattggaata tgatggggat tgctccggat tttgaatctc
     1321 aacactattt cggtactgat tcttcagggc gtgatttgct ggttcgagtt gcaatcggtg
     1381 gacgcatttc gttaatggta gggattgccg gtgcattaat tgcggtgctt atcggtacaa
     1441 tctatggagc aatttccggt tatttcggcg gtaaagtcga tatggtgatg atgcgttttc
     1501 tggaaatttt aagctcattc ccatttatgt tcttcgtaat tttgctggtt acgttcttcg
     1561 ggcagaatat tttcctgatt ttcattgcaa tcggtatggt agcgtggctg aaccttgccc
     1621 gtattgtacg tggtcaaact ctttcgctta aaaataaaga gtttgtggaa gcagcgattg
     1681 tgtgtggcgt ttcccgccgt caaattattt ggcgacacat tgtgcctaac gttttaggct
     1741 tagtggtggt ttatgcctcc cttgaagtac catctctgat tttgtttgaa tcgttcttaa
     1801 gtttcttagg gcttggtaca caagagccga tgagcagttg gggggcatta ttaagcgacg
     1861 gagcggcaca aatggaaacc tcgccttggc tactggcttt ccctgccttt ttcttatgtt
     1921 taacactgtt ttgttttaat tttattggtg atggcttgcg tgatgccctc gatccaaaag
     1981 ataaatagag gtttagaatg gaattattag cagtaaataa tctcgatgtt tatttaaaaa
     2041 cggacgaaga attagttcac gcagtgcgta atgtctcgtt ctcgattgaa aaagggcaaa
     2101 cccttgcgat tgtgggggaa tcgggttcgg gtaaatccgt tacttcaatg gcaattatgc
     2161 agttattgcc taaaaatatc gtctcattcg gtgaacgctc aagcatcatt tttgaagaac
     2221 aaaacttgct tactctatct gaaaaagcaa tgcaaacagt tcgaggtgat cgcatcggta
     2281 tgattttcca agagccaatg acctcactca atccttatat gccgattggc aagcaggttg
     2341 ctgagacggt tcaaacccat aatccgcaaa tcagcaatgc cgaagcggaa aaaatggtgt
     2401 tggaaacttt gcaaaaagta aaaattcccg atgcggaaaa gaaaatgatt tgctatccgc
     2461 acgagttttc cggcggtcag ctacaacgaa ttatgattgc aatggcaatt atcaacaaac
     2521 ccgatttatt gattgccgat gagccgacca ccgccctgga tgtcacaacc caagcggaaa
     2581 ttttggattt aatgcacgac ttacagcgtg atatgggaat ggcgattatc ttaatttccc
     2641 acgatttacg cttggtgcat aaatacagtg acttagtctg cgtaatgcaa aacggcgaaa
     2701 ttattgagcg aggcgaaacg gaaactgtgt tcaccaatcc gcaacatcct tatacggtgg
     2761 agctacttac cccgattcca actaatttaa aaggggaatt accgccaaat gcaccaacct
     2821 tgcttcaggg tgagcatatt aaggtagatt atattttaaa acgctcgcta ttcggtaagc
     2881 cgaaaaaggt gtttaatgcg ttaaaagata tttcattgca cttgaaaacc ggcgaaacgc
     2941 tcggcattgt aggcgaatcc ggctccggga aatctaccct tgggcgtgcc attatgcaga
     3001 ttttagatta tcgcggcaat attacatttg caggtaagac actggctaat ctgaataaag
     3061 ccgaacgtca agccctgaaa aaagatatgc aaatggtgtt ccaagacccg ttcaactcac
     3121 tttctcctcg cttaacagtt ggggaaatta ttgctgaggg cttaaccgta cattgccctc
     3181 aaatgagcaa aaccgaacgg cgggaaaaag taatgaaaat gttggaagag gttaatctca
     3241 accctgcaat gattaaccgc tatccgcacg aattttcggg cggacaacgc cagcggattg
     3301 cgattgcaag ggcaatcatt ttagagccga aatttgtatt gcttgatgag ccaacctcag
     3361 cacttgaccg ctctactcag atcactgtaa ttgaattgct taaccgctta cagaaaaaat
     3421 acggcttaag ttatattttc atcagccacg atttggcggt catcaaggca cttagcgacc
     3481 gagtgattgt aatgagccaa ggtgagattg tagaaagcgg tgatgtcact caaatttttg
     3541 aaaatccaca acaagaatac accaagcggt tgattttagc ctctaatttg taaccccttc
     3601 cacaaggtct tattctacca aaaaacaaga ccttgtcatt ttatgctagc aaaacattgt
     3661 aattttgata ttttatttca attttttgaa aaaatatgga actttatata aaactcactt
     3721 tctaaacaat acgttatctg aactaagata acgcttattt aaaggaaaat atatgaaaaa
     3781 attgatgata ttcgcaacaa ctgccatgat tgtaagcaat ttagcccatg cagcaggtga
     3841 acaaagtgat gcaagagaag cccacgaaag caccgtaaaa acctcaactg ttaagtatag
     3901 ctgccaaaac ggcaaaaaat taagcgtgaa atacggcttc aataaacaag gcattccaac
     3961 ctatgctgag gcaaaattaa gcggtaaaaa acgttttatg ccgatcaacc tatacacgac
     4021 agatgcaacc ggcactaact ttggtgacga aaacaatttc agcctttacg gtgatccaat
     4081 ggaatttacc aaccaccgta aagcatcagt aaatatccaa agccccgcaa gtgaaatttt
     4141 atataaaggt tgcacacctc aaaaatagat aaaaagtaaa atgagtccgc tctgataagt
     4201 ggctttttaa tagctgttta ttgcagatag gaaagcattt tgtttttatc tactatccag
     4261 aaacaagcgg ttaaatttgt ttaatttttt acaaaaaatg ggccaaaatt aaccgcttgt
     4321 ttattactat aaagaaataa gtacgtaaaa attaactctt acatttaatg cttatctcta
     4381 aaatttaaac tccactgatg ccattacata gcgctctgga gcaaggtaac gatttaagcc
     4441 ttcgccagtt atactgttgg caactccagg actagttata ccaatagttc ttaaactatc
     4501 ccaagtccaa tattttttat taaagagatt ataaacccca actttaacaa tcgctctttc
     4561 tccaaacttt ttgtgtgcaa ctaagtcaaa tacggtggct gaattactta agtatgggta
     4621 agtggttaat gatttatcgt aacttaaata cccagtattg gctaaaatag catactgttg
     4681 tgcatatttt gcttttttgc cttgtgtgta tgtgccgttt aggtgtatcg cccagtcatt
     4741 gttcacagct cgataaccta tgccgaacaa tgcttttaac ggttgaaccg tgagtaagtc
     4801 ggtaccatca ttacgtttgc cacgagcata accaaatcca ccattaagct caaagccttg
     4861 tggaattgca ttccacgctg tgtttaagtc taaagttccg ttaatatcaa cacctttgat
     4921 tttagctcgg ttaacatttt gcgattggta aatattgtta ccaatagtgg caagatcaat
     4981 cagatttcta tagtgggtat ggtaagcggc tacgctcaag ctacctaatg aaccattacc
     5041 taccagtgaa atttcattac ctaagcttgt ttccggtttt aagttagtat ttgctaagaa
     5101 agtttggctt gctccacgcc cttgaacttc acggttaaag aacatttcac ttgcggtagg
     5161 tgttctaaaa ccacggctga gtttatagcc aatttgccat acaggagaga gctgataatt
     5221 taaaccaata tctcccgccc aattgttaaa ggttttatct gacaaactat tatcatcaga
     5281 atgtaatttc tgactgctca atcgagcatt taaatgagca gatagatttt gtgtcagtaa
     5341 aatagagtct cccatcaaaa tatggaaaga cttggttttt gccggagact gcatattttg
     5401 atcggttggc gtaatacctg ctgtataggt ttgcatatca aaactgaaat cagatacacc
     5461 atagcctaat tccgttgaca atttatgttc ggtattacct agatcaacag gcttgaatgt
     5521 aacacctaag ctaccttcaa catttttagt attaaaagcc cgtttggtat tatctgtttt
     5581 tgtaccacgg gcaatagtga tttgatcgtt aatacctgaa atttcagtat cctgtttggt
     5641 taccgcaact ttaatatttt caagaatact gttttttgga gtgtataaat aagtaacgcc
     5701 ataagtttca atttcctgca catccaaaaa atcacgtaat gtgacggtag aataagaaag
     5761 ctcttgaccg gcgtttttac tgcgttgctt agaagttttt aattcaaggc gattttcatc
     5821 attccatttt agccctaatt ttgccaagta gctgtattga ttatttttgt aaggatttgg
     5881 taaaccacga ccacttccca taatatccgg acctttagac gttgtttgtg tttcgtgtcc
     5941 ttgacggtgg ctatacataa ccaaagcatc cgcatattga ccatccgcag caatacctgc
     6001 cgtctcaata ttctctttat tttgaccgtt ataggtatat ttcaacatcc caccaaattg
     6061 gcgacctttc attaaaatat cacttacggt tttagtttta tagttcacag agccgcctaa
     6121 tgccccattg cctacttttg tgcttgagcc tttttcaatg gttacatcgc tcattaattc
     6181 agtatcgatt tcagggcgag atttattgta gtaactatag cgactaaagg tggcatagtt
     6241 atttgatgct gcttgcgata tgccatcaac tgttactgcc acttgattac cttcaacacc
     6301 acggatatta aacccttgtg ggccaaagcg gcctgtttgc ggaacgccca tatcagtact
     6361 ataacgtact aaatcttcat tgctcatcac cgcttgctgt ttaagtaggt tctcctgctg
     6421 ctcatttact tcaatagtat ctagttcaat gctactaatc tttgtttctt gagctaagga
     6481 gagttgagaa ataaagggag taatcgctat tgctaataca gtttgcttta tttgcatatt
     6541 ttagatcctc tagttagtt
//
LOCUS       DANLUA010000138         6554 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLUA010000138 DANLUA010000000
VERSION     DANLUA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            359..2890
                     /locus_tag="PXB44_001980"
     CDS             359..2890
                     /locus_tag="PXB44_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282429.1"
                     /translation="MQREYEKLGASISSVSRKRTEENRALKDSRHQQAKQLLYFRSLR
                     RELRASGINIKAFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKARNEKYWATVEKV
                     KNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNAILEVTEGSAEK
                     AKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDTGAAMGKPVMQA
                     VEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKVNKNNRKEIEQT
                     LTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWIKNKLQWVLDSL
                     DKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARENKGLIATFVKW
                     SAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALFDSNDKFRLFNK
                     SLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTKVFWLNMLKGIL
                     GKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRAFFGGFLQGLSK
                     GLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKASSAGEKFGLAL
                     AGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEHAKQITKTAEMS
                     NNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGIVHKGEYVMTKE
                     ATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTASQPSPTVAPVS
                     QNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            2955..3131
                     /locus_tag="PXB44_001981"
     CDS             2955..3131
                     /locus_tag="PXB44_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282430.1"
                     /translation="MFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQK
                     SQELNEFQREVVYG"
     gene            3124..3411
                     /locus_tag="PXB44_001982"
     CDS             3124..3411
                     /locus_tag="PXB44_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282431.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVIILAIIHSSRLYPRP"
     gene            3540..4145
                     /locus_tag="PXB44_001983"
     CDS             3540..4145
                     /locus_tag="PXB44_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5282432.1"
                     /translation="MSEPNHAEQIRRLDNIIRLGTIAEVDLTTATARVNSGGITTDFL
                     PWLTFRAGTSQTWSAPTVGEQCVVLSVSGEFTTGVILFGIYTQNAPSQSADEHLFRFS
                     DGAEISYNTASGQLTVKNCKVVIVQATESITLDTPTVKATKNVVIGGNLSVKGTTSSQ
                     GAISTQGAVTAKGDVSGAGISLGSHHHIEQGDGRPTSGAKP"
     gene            4145..4480
                     /locus_tag="PXB44_001984"
     CDS             4145..4480
                     /locus_tag="PXB44_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5282433.1"
                     /translation="MNRETGEQLEDEVEHIKQSIKDILLTAKGSRVMRRTYGSNLYKL
                     TDKPIAASLIMQLSAACVMALQQWEPRIAITRFKVEFAEDNYNKLICTLDFTLKHRKI
                     SIKGMRFTL"
     gene            4477..5394
                     /locus_tag="PXB44_001985"
     CDS             4477..5394
                     /locus_tag="PXB44_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDL5282434.1"
                     /translation="MSEIVDLSKLPAPKVLEELDYETLLAERKAKFLSLYPESERAVM
                     AARLALESEPITKLLQENCYLQLLERQRINSAAQATMLAYATGTDLDVIAANFNVERL
                     VVQAEDLTASPPIVEELEPDDDFRQRVQLKIESVSVAGPRKAYTFHALSANGKIADVS
                     VISPQPAYVTVTLLSRDGNGAVASEIINAAKIALNDEEVRPIADRVTVQGASIVEYEI
                     NAVLHLYRGPEKEPILKAAKANLEAYINQTKRIGRDVTHSGIHAALHVAGVQNVEILQ
                     PRTDLVLTPYQAGYCVNYTLNVEVSDEYS"
     gene            5381..6013
                     /locus_tag="PXB44_001986"
     CDS             5381..6013
                     /locus_tag="PXB44_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDL5282435.1"
                     /translation="MNTVKALLPPGSSLLEKRAAECLQQAVQNPIKFANLINPHKCPV
                     KFLPYLAWALSVDYWEEHWSEELKRETIIRSFEEHRKKGTASAVRRIVESLGYAFEIR
                     EWFAEKRQRQPGTFRVFVELKDKGISEKIYQELERLIEDAKPVSRHITELAVSATSKG
                     KANIFSGAHSGEIITIYPPKKLPVIVIKSKMDLFSATNSGEIITIYPNKR"
     gene            6016..>6554
                     /locus_tag="PXB44_001987"
     CDS             6016..>6554
                     /locus_tag="PXB44_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282436.1"
                     /translation="MEQKYKTILTHHGERVIVEALANKIPVPLKEMAIGDGNGSSITP
                     SASQTTLVREVYRAEITDLLEDPQNRHQMIAELLIPENVGGFIVREIGLFDEQGGLVA
                     VANCPENYKPVLEQGSGKVQYYRMILQVSSSDAVTLSINNNIVYATRTEFNEFVNNLS
                     SPDGYKHIGRCKSVAELRT"
BASE COUNT         2174 a         1230 c         1490 g         1660 t
ORIGIN      
        1 agtagttctt tctgcgttag ataaattaac cgcaccattt aagagtgtgg taaaacagac
       61 ggagaaatta tctcaatctt taaaagtaca gagagatgaa ttaaaagcat tggaaaaaac
      121 acaaagtaaa atgcaatcat ttaaaaaaat ggacgatagc attaagaaat ccaatgaaac
      181 tatactcaaa cagacaaagc atttagataa tctcaaaaat aaaatcagcc aaatgaaaaa
      241 tgaacgaatt gagctgaaag tgaaaatgga tgcgaaaaaa agggaatttc aacaaattac
      301 gaaaggtggt tcagcttgga gtgcaagagc cgcattttta gataaagatt ttgaaaaaat
      361 gcaaagagaa tatgaaaaac tcggtgcatc tatttcaagt gtcagccgaa aaagaacaga
      421 agaaaatcgg gcattaaagg attctcgcca tcaacaagct aagcaacttt tatattttcg
      481 tagtttaaga cgtgaattga gagcaagcgg cattaatatt aaagcttttg ctcaaagcga
      541 aaccaactta acagacaaga ttgcaaaagc aaatcaatta atcgaccaac aaaaagcaaa
      601 attagaaaaa ctaaatgcgg ttaaagctcg aaatgaaaaa tattgggcaa ctgtcgaaaa
      661 ggtaaaaaat accagcgaac gtttgcataa tttaggacaa cgctcaatga ttagtggtgc
      721 ggcaatttct gcccctgttg ttgggctagg gaaaggggtt gtcggaatgg ctcaaacagc
      781 gggtaaattt gaacaattta atgctatttt agaggtaacc gagggaagtg ctgaaaaagc
      841 caagcaaagt tttgattggg ttaaacaatt tgcagtagat accccctcaa accttgatga
      901 agcaatggaa gcctttgtaa agttaagagc atacggtctt gatcctacca atggcttact
      961 acaaacatta ggtgatacag gtgctgcaat gggtaagcct gtaatgcaag cagttgaggc
     1021 aatagcagat gctttaacgg gtgagaatga acgcttaaaa gaatttggta ttaaaggaag
     1081 tgtagttaaa ggtacaaata ttattgagta tgcctacact gataaattag gcaaacaaca
     1141 agtcgcaaaa gtaaacaaaa ataaccgaaa agaaattgag caaaccctaa cccgtatttt
     1201 taacgaaaaa tatgctggag caatggaaaa acaagctaaa acgctagttg gtatttgggc
     1261 aaaaattgaa gattattgga caaatttcca aatgcaagtg atggaatcgg gagcatttga
     1321 ctggattaaa aataaattgc aatgggtgtt agacagttta gataaaatgg ctgaaaatgg
     1381 cgagttgcaa aaatgggctg aagatgtcgg agccgttatt caagaaacag tacaaggctt
     1441 atgggctttt ggtgaaaaaa ttattgaagt ggttaaatgg ttagcgagct tggcacggga
     1501 gaataaaggg ctaattgcaa cattcgttaa atggtctgct attcttggct ctagtttaac
     1561 ggtgctagga gcttttgcag tggtggcaag ttttgcatta tatcctgtag caagattggc
     1621 tcttggtatc ggcaaattta caggtgcaag caccttatta aataaggcat tatttgatag
     1681 taatgataaa ttccgattat tcaataaatc actttattca tcaggcacta cggctgcttt
     1741 tgttaaatca aaatttaatc agttagcaac tttaccaaat gtgctgggaa gtagttttaa
     1801 taaattaggc ggtggcatca aaatgctaat tccacagctt aaaacaaaag tattttggct
     1861 caatatgcta aaaggtattt taggcaagtt gtggagtgca atccgtttta ttttctcacc
     1921 gttgaaatta ttacttggta ttttctcgcc gattggctta gcgattacag ctatttcggt
     1981 agcaggggta gcgttatata gaaattggga gaaggtcaga gccttttttg gaggcttttt
     2041 gcaaggatta tctaaaggtc ttgcaccagt attagagaga tttaaacctt taggaaaact
     2101 atttgaaact atatcaaact gggttaaatc tacgtttaag tggttatccg atttattaac
     2161 cccagcggat aaaacatcgg aaagtttaga taaagcgagt tctgcaggag aaaaattcgg
     2221 gcttgcattg gctggtgcaa tagacttggt aacatcacct gttcagtggc tagtcgatca
     2281 gtttaagtgg atttctgaaa atatgccgag ttgggacact attggtgagg gtgtaaaaaa
     2341 ggcagctact gcaattgttc ccaccgagca cgccaagcag attactaaaa cggcagaaat
     2401 gtcaaacaat atgtatgacc ccaattatac tccaccacaa cttgagcaaa aatggattgg
     2461 tggcttggtt ggtaacggta aaggaagagg ttttgccaca ggcggctaca ccggcaatgg
     2521 tggcaaatat gagcctgccg gtattgtgca taagggcgag tatgtaatga ccaaagaggc
     2581 aactgcacgc ttgggtgtgg caaatcttaa ccggttaaat tatggcaaag ttgctgggct
     2641 aactgcactt gcgtcaagtg ttgcgtttgc tcaaccaatg cctgcggtca aaatcgacag
     2701 cagaccaccg ctgacggcaa gccaaccaag cccaacagtt gcaccggtgt cgcaaaatat
     2761 ccatatcacc atcaacgcca ccggtgggca agacccacaa gcaatcgccc gactggttgc
     2821 aatggagttg gaaaaacaac aacgccaagc acaagcaaga gcaagatcgt ctttgagaga
     2881 caggggctaa tttgacaaag tagaatgaaa agcggacaat gctctaaatt gtccgctttt
     2941 tataggagca aatgatgttt aaagaaaaag gatatgatga atttgtgaat gaatgtgtgc
     3001 gaaaaggtag agaagaacac aaagctggtt taggatttac gctagagcaa tctcgcttac
     3061 atatcaaaaa aaccattgag caaaaatcac aagagctaaa cgaatttcag cgtgaagtgg
     3121 tttatggcta aaattaccat taaaccacaa gcattgagtc aatttgacga gattgtaaaa
     3181 agtgttatcg aatttaccgg ttttgaaacg agtggtattc gattacaaag tgatattttt
     3241 gacaaaatag agcaaattgc atttatgcca ctctctggaa gagcaagaga agatgggaca
     3301 agagaaactt tcgtcagacg ttatcgtatt gtgtacgatt acgataggca ggcagatgaa
     3361 gtaattatct tagctatcat tcacagtagc cgactttacc cacgcccata atcagtaagc
     3421 gacctaatcg gtcgcttttt ctttgcctgt taaaacccat ttcacacctc gcatttatac
     3481 ccaaagcctt tcgccttttg caaaatatcc tcatttttta accgcttgta attgtaaaaa
     3541 tgagtgagcc aaaccacgcc gaacaaatcc gccgtttaga caatattatc cgacttggca
     3601 ctattgccga ggtggattta accacagcga ctgcacgggt caattcgggc ggtattacga
     3661 ccgatttctt accgtggctg acttttcgtg ccggtacatc gcaaacgtgg tcagctccaa
     3721 cagttggcga gcaatgcgtg gtgttaagcg tgagtggtga atttaccaca ggcgtgatct
     3781 tatttgggat ttacacccaa aacgccccga gccaatccgc cgatgagcat ctattccgct
     3841 tttcggacgg tgcagaaatc agctacaaca ccgcaagcgg tcaattaacc gtaaaaaatt
     3901 gcaaagtggt gattgtgcag gcgacagaat ctatcacgct cgacacgccc acggttaagg
     3961 ccacaaaaaa tgtcgtgatt gggggcaacc tatccgtgaa aggcacaacc tcatcgcaag
     4021 gtgcaatcag cacacaaggg gcggtaacag caaaaggcga tgtatcaggg gcgggaatca
     4081 gtttaggcag ccaccatcac attgagcaag gtgacggcag accaactagc ggagcaaaac
     4141 cataatgaac cgagaaacag gcgaacagct tgaagacgaa gtagagcata tcaagcagtc
     4201 tatcaaagat attttgctga cggcaaaagg cagtcgggta atgcggcgaa cctatggcag
     4261 taatctctac aagctaactg ataaaccgat tgccgcaagt ctgattatgc aactctccgc
     4321 tgcgtgcgtg atggcgttgc agcaatggga gccacgtata gcgatcaccc gatttaaagt
     4381 ggaatttgcc gaagataact acaataagct gatttgcacc ttagatttta cccttaagca
     4441 ccgaaaaatc agcatcaaag gaatgcgttt tacactatga gcgaaattgt cgatttatcc
     4501 aaactccccg ccccgaaagt actggaagaa ttggactatg aaaccttact ggcagagcgg
     4561 aaagccaagt ttttatccct ctacccagaa agcgaacgag cggtaatggc tgcacggtta
     4621 gcgttggagt ctgagcctat caccaagtta ctacaagaaa attgctattt gcagctactc
     4681 gaacgccaac gcattaatag tgcggctcaa gccacaatgc ttgcttatgc taccggtacg
     4741 gatttagatg tcatcgcagc caactttaat gttgagcggc ttgtagtgca agctgaagac
     4801 ttaactgcaa gcccgcctat cgtcgaagag ttagagcctg atgatgattt ccgtcagcgt
     4861 gtgcagttaa aaattgaaag tgtttcggtt gccggtccac ggaaggctta cacctttcac
     4921 gccctgtctg ctaacggaaa aattgccgat gtgtccgtca tatcaccaca gccagcttat
     4981 gtaacggtaa cgctgctttc tcgtgatggc aacggagccg tagcctcaga aatcatcaat
     5041 gcggccaaaa ttgctctcaa tgatgaagag gttagaccga ttgccgatag agtaaccgtg
     5101 caaggggcaa gcattgtgga atatgaaatt aatgcggtgt tgcatctcta ccgtggacct
     5161 gagaaagagc ctattttaaa agcggcaaaa gctaacttag aggcttacat caaccaaaca
     5221 aaacgcattg gacgagatgt gactcactca ggtatccacg ccgccttgca cgttgccggt
     5281 gtgcaaaatg tggaaatttt gcagccgaga actgatttgg tgctgacacc ttaccaagcc
     5341 ggttattgtg tcaattacac gctaaatgtt gaggtgagcg atgaatacag ttaaagcatt
     5401 attgccaccc ggttcatcac ttttggaaaa acgagctgcc gaatgtttgc aacaagcggt
     5461 acaaaatccg attaaatttg caaatttgat taatccacac aaatgccccg tgaaattttt
     5521 gccctatctt gcgtgggcct tatcggtgga ttattgggaa gagcattgga gtgaagaatt
     5581 aaaaagagaa accattattc gatcttttga agaacaccga aagaaaggca cagcaagtgc
     5641 agtgcgaaga attgtggaat ctctcggcta tgcttttgaa atccgtgagt ggtttgcgga
     5701 aaaacggcaa cggcagcccg ggacgtttag ggtttttgtt gagttaaaag ataaagggat
     5761 tagcgaaaaa atctaccaag aattagaaag gttgattgaa gatgcaaaac cggtatcaag
     5821 gcacatcaca gaattagcag tatcagccac ctcaaaaggc aaagcaaata tttttagtgg
     5881 agcacacagt ggcgaaatta ttacgattta tccacctaaa aagctccctg ttattgtcat
     5941 taaaagcaaa atggatttat ttagtgcaac aaacagtggc gaaattatta cgatttatcc
     6001 taataagagg taaaaatgga acaaaaatat aaaacaatat taacccatca cggtgagcga
     6061 gtgatcgttg aggctcttgc aaataagata ccagtgccac ttaaggaaat ggctatagga
     6121 gatggtaacg ggtcatcaat aactccaagt gcatctcaaa ccacacttgt aagagaagtt
     6181 tatcgtgcgg aaattacaga tttgctagaa gacccgcaaa atcgccatca gatgattgct
     6241 gaattactaa tcccagaaaa tgtaggtgga tttattgtcc gagaaatagg gttatttgat
     6301 gaacaaggtg gtttggttgc tgtagcaaat tgccctgaaa attataagcc tgtattagaa
     6361 caaggcagtg gtaaagtcca atattaccga atgattttac aagttagtag tagtgatgcg
     6421 gtcaccttat cgattaacaa taatattgtt tacgccacaa gaactgagtt taacgaattt
     6481 gtaaataact tgagtagtcc tgatggctat aaacacatcg gacgctgcaa atctgtagca
     6541 gaactccgca ctat
//
LOCUS       DANLUA010000139         6530 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLUA010000139 DANLUA010000000
VERSION     DANLUA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            119..2776
                     /gene="aceE"
                     /locus_tag="PXB44_001988"
     CDS             119..2776
                     /gene="aceE"
                     /locus_tag="PXB44_001988"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL5282437.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
     gene            2980..4890
                     /gene="aceF"
                     /locus_tag="PXB44_001989"
     CDS             2980..4890
                     /gene="aceF"
                     /locus_tag="PXB44_001989"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL5282438.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            5090..6514
                     /gene="lpdA"
                     /locus_tag="PXB44_001990"
     CDS             5090..6514
                     /gene="lpdA"
                     /locus_tag="PXB44_001990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL5282439.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
BASE COUNT         1993 a         1233 c         1511 g         1793 t
ORIGIN      
        1 attgttgttt tactgaaatt ttttcgataa gaattggtct aaaaatcacc aaacaaacaa
       61 aatttgttaa aattttgtta tttatcacct caatcgttaa tttataagga agaatactat
      121 gtcagagaac ttaagaaatg acgtagatcc aatcgaaact caggattggt tagcatcact
      181 cgattcatta attcgtgaag aaggtttaga gcgcgctcaa tttatcattg aacaagttat
      241 tagccaagct cgtgcaggtg gcgtggcatt accaacaggc gtaacaacag attatgtcaa
      301 tactatccct gtttccgagc agccagctta tccgggtgat cataaaatcg aacgtcgtat
      361 tcgttctgcg gtacgttgga atgcgattgc ggcagtactt cgtagccaga agaaagatct
      421 tgacttaggt ggtcatattt caactttcca atctgcggca actatgtatg aagtgtgttt
      481 taaccacttt ttcaaagcac caaccgaaaa agatggtggt gatttagtgt tcttccaagg
      541 ccacgcagca ccgggtatgt atggtcgtgc gttcttagaa ggtcgtatta ccgcagaaca
      601 aatggataac ttccgtcaag aagcatttac agacggttta tcttcttacc cacaccctaa
      661 attaatgcct gaattttggc aattctctac cgtatctatg ggtttaggac cggtaaatgc
      721 tatttaccaa gctcgtttct taaaatattt agaaaaccgt ggtctaaaag atacttcggc
      781 tcaaaaagta tatgcattct taggcgatgg cgaaatggat gaaatcgaat ctaaaggtgc
      841 attatcattc gccggtcgtg aaaaattaaa taacttaatc ttcactatta gctgtaactt
      901 acaacgttta gacggtccgg ttaacggtaa cggcaaaatc gtgcaagaat tagaagcact
      961 ctttaccggt tgtggctggg aagtgattaa agtgttatgg ggtagtggtt gggataaact
     1021 gcttgaaaaa gatacttcag gcaaacttaa acagttaatg atggaagtgc ttgatggtga
     1081 ctatttaacc ttcaaatcca aaaatggtgc ttacgttcgt gaacacttct tcggtcgtta
     1141 ccctgaaact gcagcattag ttgctgatat gacagacgat gaaatttggg cattacgccg
     1201 tggtgcacac gattctgaaa aactttatgc agcctatgca aaagcacaaa aatcggacaa
     1261 accagttgta attttagctc accaagtgaa aggctaccgt attcctgaag cggaaagtaa
     1321 aaatactgct caccaatcga aaaaaatgtc attagacagc ttaaaaggct tccgtgacta
     1381 cttcgagcta ccattaaccg atgagcaagt agaaagccta gagtatgtaa gattccctga
     1441 aggttctgaa gagtacaact acttacacgg acaacgtaaa gccttaaacg gttatgtgcc
     1501 tgtacgtaaa ccgaaattta cggttgattt caaagtgcct gaattaagcg aattccaagc
     1561 attattggat gagcaaccac gtggtatttc aaccacaatg gcgttctctc gtgtattgaa
     1621 tactttattg aaagataaaa atatcggtca gcaaatcgta cctattattg cggacgaagc
     1681 ccgtactttc ggtatggaag gtttattccg tcaaatcggt atttataacc cacatggtca
     1741 aaactatgta ccgtcagacc gtgatttagt agcttactac cgtgaagcaa aagatggcca
     1801 agtgttacaa gaaggtatca atgagttagg tgcaacagcc tcttgggtgg cagcagcgac
     1861 ttcttattct gttagcaact taccgatgat cccattcttc atctactact caatgtttgg
     1921 tttccaacgt gtaggcgata tgatgtggtt agccggtgac caattagctc gtggctttat
     1981 gattggtggt acttcaggtc gtacaacatt aaacggtgaa ggtttacaac acgaagatgg
     2041 tcatagccat attcaagcag gtgtaatccc gaactgcgtc acttatgacc ctgcatttgc
     2101 ctttgaggtt gcggttattg ttcaagacgg tattcgtcgt atgtatggtg aaaaccaaga
     2161 agatatcttc tactacatta ctaccttaaa tgaaattacc gaacagccgg caatgccggc
     2221 aggtgcggaa gaaggtatcc gtaaaggttt atataaattt gaaaccgttg agggtaaagg
     2281 taaaggtcac gtacaattat taagctcagg tgcaattatg cgtcacgtgc gtgcagctgc
     2341 acaaattctc gcgaatgact acggcattac agcagatgta ttctcagcac catcattcaa
     2401 tgaattaggt cgtgatggag cagatgtggc tcgttggaac ttattacacc caactgaaac
     2461 accacgtgta ccgtacgttg ctcaagtgtt acaagattta ccgactgttg cttcaaccga
     2521 ctatatgaaa atgtacgcag agcaaatccg tgcttatgta ccaagtaaac actatcacgt
     2581 gttaggtaca gacggtttcg gtcgttcaga cagccgtgca aacttacgtg aacacttcga
     2641 agttgatgag cgttatgtag tagttgcagc cctcgctcaa ttagcgaaag aaggtacagt
     2701 tgaaaacaaa gtggttgcgg aagctattac taaattcggc ttaaatgtag atcgtattaa
     2761 cccattatat gcgtaatttc taatactctc tcccttgatg ggagagagac agctaaaggt
     2821 ttcgtttaga aacttttagc agagagggta ttagttttat ttgcaaaaat gttgagaaaa
     2881 tcaaccgctt gcattccctt ctccctgatt tttcttctta caaagaaaaa tctgtccctc
     2941 ccacaatggg agagggggta actcaagaag gatttcaaaa tgtcaaaaca aattaatgtg
     3001 ccggacatcg gttcggatga agtcagtgtg actgaagtga tggtaaaagt tggtgatact
     3061 atttcagtag atcaatcaat tattaacgta gaaggcgata aagcctctat ggaagtgccg
     3121 gctcctgaag caggtgtggt aaaagaagtt ttagtgaaag tgggtgataa agtttcaacc
     3181 ggttcaccaa tgttaatttt agagtcagca gatgctgctc ctgctcaaga agcaccaaaa
     3241 gcagaagcag ctcctgctgc tccagtagca acaacttcag ccgttgttga agtaaatgta
     3301 cctgatatcg gtggcgatga agttaacgta accgaaatta tggttaaagt aggtgatagc
     3361 gttgaagtgg atcaatctat catcaacgtt gaaggtgata aagcctcaat ggaagtgcct
     3421 gcaccggttg ccggcgtagt aaaagaaatc ttaatcaatg tgggtgacaa agtttcaact
     3481 ggtaaattga ttatgaaatt tgaaacggct tccgcagcta cagcacaatc tacaccggtt
     3541 gcggaagctg ctccagctca agcttcggca tcagcgatta aagatgtaaa tgtaccggac
     3601 atcggtggtg atgaagtaaa cgtaactgaa attatggtta aagtgggtga ttctgtttca
     3661 gaagaacaat ctttaatcac ggttgaaggt gataaagctt caatggaagt tccggctcca
     3721 tttgccggtg tggtgaaaga aattttagtg aaatcaggtg ataaagtttc aaccggttca
     3781 ttaattatga aatttgaagt agcgggtgcg gcaccggctc cggcggctgc tcaagcggct
     3841 tcagctcctg ctcctgctcc acaagcagca gctccggcga aagctgacgc agctccggca
     3901 ggtcaaaacc aatcaggttt aagccaagaa caagtggtgg cttcagctgg ctatgcacac
     3961 gcaacaccgg taattcgccg tttagctcgt gagtttggtg taaacctaga caaagtaaaa
     4021 ggtacaggtc gtaaaggccg tatcgttaaa gaagatattc aagcatacgt taaaacggct
     4081 gttcaagtat ttgaaaaaca aggcggaaca gcagcggcag caacaggtgc ggcaaacggt
     4141 gcaggcttag gtttattacc gtggccgaaa gtagatttca gcaagtttgg tgaagttgaa
     4201 gaagttgaat taagccgtat caataagatt tccggtgcga acttacaccg taactgggta
     4261 atgattcctc acgttacaca tttcgatcgc acagacatta ccgagttaga aaacttccgt
     4321 aaagagcaaa ataaagaagc ggaaaaacgt aaattagacg tgaaaattac ccctgtggtc
     4381 ttcattatga aagcagtggc aagtgcatta gaagccttcc ctcgtttcaa cagctcaatt
     4441 tctgaagatg cacaacgttt aaccttgaaa aaatacatca atatcggggt tgcggttgat
     4501 acgccaaacg gtttagtggt gccggtgttc aaaaatgtga acaaaaaagg cattatcgaa
     4561 ctttctcgcg aattaatgga agtctcgaaa aaagcccgtg aaggtaaatt gacagcatct
     4621 gatatgcaag gtggctgttt cacaatttca agtttaggcg gtattggtac aacgcacttt
     4681 acgccaatcg tgaatgcacc ggaagtggct attttaggtg tgtctaaatc tgaaatgcaa
     4741 ccgatttgga atggcaaaga gtttgaacca cgcttaatgc ttccgctttc attatcattc
     4801 gaccaccgtg tgatcgatgg tgctgatggt gcgagattct taagctatat taatagcgtg
     4861 ttggcagata ttcgtcgttt agtgatgtaa ttcggtaaag ctcctcctaa aaaagtccct
     4921 ctttagaaaa gaggggtagg gggagatttg atagacgtaa tttacgaaga gaataaatta
     4981 tttggtagaa atttgtgttt gattgattgc tctctcactt cgctttatat tctgccaaat
     5041 ctcccttacc cctctttgct aaaggggggg aataaaaaga ggaaaaacta tgagcaaaga
     5101 aattaaaacc caagttgtcg tacttggtgc aggtccagca ggttactcag cggcattccg
     5161 ttgtgccgac ttaggcttag aaacagtaat tgttgagcgt tattcaacct taggtggtat
     5221 ttgcttaaac gtaggttgta tcccttctaa agcattatta cacgttgcaa aagtaattga
     5281 agaagctaaa aatgcagtac acaacggtgt gacctttggt gagccaacga ttgatttaga
     5341 tcaggtacgt gctggtaaag aagccgttgt ttcaaaatta accggtggct tagcaggaat
     5401 ggctaaggct cgtaaagtga cagtagtaga aggcttggct gcatttactg gtccgaatac
     5461 gttagtcgct cgtgatcgtg acggcaatcc tacaactatc acgttcgata atgcgattat
     5521 cgcagcaggt tctcgtccga ttcaactacc atttattcca cacgaagacc cacgagtttg
     5581 ggattcaacc gatgctctta aattaaaaga agtaccgaaa aaactcctta ttatgggtgg
     5641 tggtattatc ggtttagaaa tgggaacggt atataacgcg cttggttcag aagtggaagt
     5701 ggttgaaatg ttcgaccaag taattcctgc tgcagataaa gatgttgtag cgatttacac
     5761 caaacaaatc gagaaaaaat tcaagctaat gcttgaaacc aaagtaaccg cagtagaagc
     5821 taaagatgac ggtatctatg tatcaatgga aggtaaggct tgcaacgata ctaaacgtta
     5881 tgatgcggta ttagtcgcta tcggacgtgt gccaaacggt aaattaattg atgccggtaa
     5941 agctggagtg gaagtagatg agcgtggctt tattcgtgta gataaacaaa tgcgtaccaa
     6001 tgtgccacac atctttgcta ttggtgatat tgtcggccag ccgatgttag ctcacaaagg
     6061 tgtacacgag ggacacgtgg cagcagaagt gattgcagga atgaaacatt acttcgatcc
     6121 gaaagtcatt ccgtccatcg cttatactga accagaagtg gcttgggttg gtaaaactga
     6181 gaaagagtgt aaacaagaag gcttgaacta cgaagtggcg aaattccctt gggctgcttc
     6241 aggtcgtgcg attgcttctg aatgctcaga aggtatgacc aagttaattt ttgataaaga
     6301 tactcaccgt gtattaggtg gtgcgattgt tggtagcaat ggtggcgaat tattaggcga
     6361 aatcggtctt gcaatcgaaa tgggctgtga tgctgaagat atcgcattaa ccattcatgc
     6421 tcacccaacg ttacacgaat cggtaggatt agcggcagaa gtatttgaag gttcaattac
     6481 agacttacca aatgcgaaag ccaaaaagcg ttaatcagat tatttaatct
//
LOCUS       DANLUA010000140         6514 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLUA010000140 DANLUA010000000
VERSION     DANLUA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..189
                     /locus_tag="PXB44_001991"
     CDS             <1..189
                     /locus_tag="PXB44_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5282440.1"
                     /translation="ADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCLNGCE
                     LRGNQRNFGGFVSVNKLG"
     gene            193..924
                     /locus_tag="PXB44_001992"
     CDS             193..924
                     /locus_tag="PXB44_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5282441.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEAQGELVAVVHSHPEPNGKPVLSTLDRKMQVQTGLDWWLVHNQKIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQG
                     QGFERVETPQIGDVILMQVGADVPNHAAIYIGDQMVVHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLAEKLDFDGILNDIAVNN"
     gene            1193..1822
                     /locus_tag="PXB44_001993"
     CDS             1193..1822
                     /locus_tag="PXB44_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5282442.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            1832..>6514
                     /locus_tag="PXB44_001994"
     CDS             1832..>6514
                     /locus_tag="PXB44_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282443.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTQIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKY
                     SSQYLESVVFDNLPPVPFNISVERLTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAV
                     AGISFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKVAWTNNPAWEI
                     YDLAPILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGMEPRFTCNVWLTEVKTAYD
                     LLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVGGFERSYSARKSRHNAVQ
                     VTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSRGQAHRTGKWILETEKRE
                     KETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASY
                     FTYINGEATHSSKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENA
                     DGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAVEVSTGYDGKLYITGDIS
                     SGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYTVIIYGKNAKGQIVTEKT
                     QTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTAR
                     TYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNIV
                     NEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGKLYTWNGTAYSATVAAED
                     VTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNRT
                     KAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKADNALSGLEEEKT
                     ARANADKAESQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQNLNAKIDNINIG
                     GRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNGPVGIVSSNRHSTSYFQQ
                     GETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETEFNHYKLTFKAPFTTQQG
                     YVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESAVSAVSADLTSYKQTQAA
                     TDSAQAQQLNQLSVNLTKAETNFNAKITEEKKARVDADKVNADKLTDITSRVANAEST
                     ITNFQSTKANKSEVASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWGG
                     SSRVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAGF
                     TRTGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFEA
                     TDWFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQISG
                     LTTRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTEGSLSRLETAKANKTEVA
                     SIAQSSLQS"
BASE COUNT         2034 a         1369 c         1503 g         1608 t
ORIGIN      
        1 gcggacacct gcacttggat ttaccgctcg gcggaatgtg gctacaccgg caagcagtat
       61 tttgatgaaa aagacaaggc aacatcagac ccaaaagccg ataaatgcag ccattgcctg
      121 aacggttgtg aattgcgagg caatcagcgt aattttggtg gatttgtatc ggtgaataaa
      181 ttggggtaat gaatggaatt ggaacaacaa ataatcgact acgccctcca acacgaaccg
      241 cacgaaatgt gcggctttgt tgtttttgac ggcaataaaa atcgatttat cccttgcgaa
      301 aaccaagcgg aagacaaagc caattacttt gaaatctctg atcttgatta catcaaagca
      361 gaagcacaag gcgagctggt ggcggtagtt cattcacacc ctgagccaaa cggcaagccg
      421 gtactctcta cccttgatcg caaaatgcaa gtacagaccg gtttagattg gtggctggta
      481 cataatcaaa aaatccataa attcaggaat gtgccgcatt taatcgggcg tgagtttaaa
      541 cacggtacaa tggattgtta cacgctctac cgtgatgcct atatgcttgc cggttacgag
      601 atggacgagt tcgagcgaca agatgactgg tggcataccg ggcaaaatct ctatttggat
      661 aatattcaag ggcaaggctt tgagcgagtg gaaaccccac aaatcggcga tgtgatttta
      721 atgcaagtag gtgctgatgt gccgaaccac gctgccattt acattggcga tcagatggta
      781 gtccatcata gccccaaccg attatctaag cgtgatttat acgacggcta ctggttacgc
      841 catacccaca gtatttggcg gcataaactg gcggagaagt tagattttga tggcatattg
      901 aatgatattg ctgtaaataa ctaacaaaaa atgaccgctt gtaacaaaaa caaaccccga
      961 agcgtttgca gcacttcggg gtttttcata tccacttacc taactaagag gacataaatt
     1021 aaatgaatga taaacgattt acattcaaat ttctaggagt tcttatggaa gcaataaata
     1081 ttaccccaaa agaaatcaga aaaacaatgt ggacagccgc aataattctc tttttgtttg
     1141 cgttcatctg gaaagcccct gaatttatca ctgctattcg ttggtggtaa ctatgattac
     1201 agttaaattt tacggacacc ttaaaacatt cggcacagat tttaaaattt tagttaaaga
     1261 catcgctgag gcaattcgtg ctctgtgttc acagcttaaa ggattgcgtg aagcgttgcg
     1321 agacggtgtt tataaagtcc gtatcggtaa gcagtattta gacccatcag cccttgaaaa
     1381 agggcttttt tattgcctga agaaagggca aaccattcat tttacccctg ttgtcaaagg
     1441 agcgaaaagc ggtggtattt ttcaagcggt gttaggtgtg gcgttaattg gggctgcatt
     1501 tgcgttaggt cctatcggct taggattggt tggctctgct acagcaatga cagttggagc
     1561 aatgggggca tcaatgttac taggaggagt ttcccaaatg ctcaccaaaa tgccaaaagc
     1621 ccccacaatg ggtaatgaaa cagaaaaaga aagctcaacg gctttttcaa atttaaataa
     1681 cttggtggct caaggcaaac ctgtgccgtt ggcttatggt ttgattcgta ccggctcgct
     1741 ggtgatatcg cagggagttg agacgatcac cattaaagaa aaccaaccgg caagcaacaa
     1801 gaaaaccggc tttagaaaat aagggataat tatgcgtatt tacggagcaa aaggtggtgg
     1861 cggcagtagc cataccccga ttgaagcacc ggaaaccggt cgctcaaaac agattgtgaa
     1921 tattgtggaa ttgctgtgcg agggcgaaat tgaaggcttg gttgatggct ttaaatcgat
     1981 ttacctagac ggcacgcaga tccaaaacga tgacggcacc tataatttta ataatgtgag
     2041 cggtcagcta aatgtcggta cgcaagatca agatgtgttg gacggttacg atagctcaca
     2101 gaatgaagtg aatgtgggtg ttgagattaa gaaaaagaat ggggctattg tccgcaccgt
     2161 aactgatgaa cgaattaacc gcttgcgttt aacgttggga gtaaggtcat tattccacca
     2221 aaataatcaa ggcgatacca ataccaccaa tgttgattta aaaatcacga ttggcacacg
     2281 gcaatattcg catagcttta acggcaaata cagctcgcaa taccttgaat ctgtggtgtt
     2341 tgataacctg ccgcctgtgc catttaatat ctcagttgag cgtctaaccg aggatagcaa
     2401 ttcgcaacgc ttacaaaacg gtacaatttg gagtagttac accgaaatta tcgataccga
     2461 gtttacctac cctaattcag cggttgccgg tatcagtttt gattcggagt attttaataa
     2521 tatcccgacc cgaaactacc taattaaagc gaaaaaagtc aaagtgccaa gcaattatga
     2581 tccagttaaa cgcacttaca ccggcttttg ggacggcaca tttaaagttg cgtggacgaa
     2641 caaccctgct tgggaaattt acgatttagc tccgatttta agcaaaatgc ttggggttga
     2701 aatcagtttt gacaaatggg cgttgtatga tgttgctcgc tattgcgatc aacttgtgcc
     2761 ggacggtatg ggtggaatgg agcctcgttt cacctgtaac gtatggctca cggaagttaa
     2821 aactgcctat gacttgttga atgatttctg ctcggtattc cgtgcgattc cgatttggac
     2881 cggaacagaa gtatcggtca ttatcgaccg tccgcgtgat ccggtttgga cttacacaaa
     2941 tgctaatgta gttggtggat ttgaacgatc ctactctgct cgcaaatcac gccataatgc
     3001 agtgcaagtt acttattcgg ataaaacaaa tggctatgaa agtgcgattg aatatgtctc
     3061 tgatgacgag gaaatcaaaa agcacggctt aaatttaagt caaattactg ctttcggttg
     3121 tacctctcgg gggcaagcac accgtaccgg aaaatggatt ttggaaaccg aaaaacggga
     3181 aaaggaaacc attactttta ccgtgggacg tgagggctta atgcacttgc cgggcgatat
     3241 tattcgtgtg gcagacagcc attatgccgg tacggaaatt ggcggacgtg tactggcgat
     3301 taatgggcga aaagtcacgt tagatcgtga aattagcatt gataacgcca gttattttac
     3361 ctatatcaac ggtgaggcaa cgcactctag caaaatccaa tcggtaaacg gcaaagaaat
     3421 tacgctggat agtacaccaa ccggcttaga aacctacggt gtttggtcgt tatcgactaa
     3481 acaaatcagc tctggattat atcgctcaat ctcgattgtc gaaaatgcgg acggcacaaa
     3541 cactatcaca gcgttacaac acgaaccgca gaaagaggca attgttgata actccgctca
     3601 ctttgtggaa acggctagaa cactctacaa agctccacaa attaacgcag tggaagtctc
     3661 gaccggttat gacggtaagc tctacattac cggtgatatt agctccggtg atggcaagct
     3721 aacctatgat attaaaatta ccaaagatgg caatttatat caatacaaaa aaggcttaag
     3781 cgatccgaac attgaactaa gtgatttacc taacggcgat tataccgtca ttatttacgg
     3841 caaaaacgcc aaagggcaaa tcgtcaccga aaaaacacaa acctttacta ttgatcgacc
     3901 tccagctccg accggtgtag tagtaacagg tggattaggg caaattaccc tcgaatggga
     3961 ttgggtaaat gaggttacgc aaaccgagat tttcgcggcc gaaaccgata atttcgccct
     4021 tgcaaaaaaa atcgcaaaag tgaccgctag aacctacgct cacacactaa aaggtaacaa
     4081 ggttgtacgc tattattggc tacgtcatac cagaggcatt aatgtcggac cgttttatca
     4141 acaacagggg gtaagaggac aaacagcggt tgatttggat gctcgattaa ccgaattaaa
     4201 cactcagtta tcacgcaata ttgttaatga ggtttttgat gttgctgcac cggcaagagg
     4261 tttagaatta gtgaaaacag tcgctaattt gaccgacaaa ggtactaaac tggcgtcatc
     4321 tcaagtttac aatcaggctg acggcaagct ctatacttgg aacggtacag catactcagc
     4381 tacagtggct gcggaagatg ttaccggcaa attatcaaaa tctaaaattg atacgtccct
     4441 tatttcgcaa ttaacaggtg cagataacac cgcaaatttg gcaagaagat tagcggaaac
     4501 agctcaaagc aacattaacc aagagattac taaccgacaa gatgccgtta ctgccgaagc
     4561 aaataaccgt actaaagcca ttcaagccga aagtgcgaat ttaacgaaaa aaattcaagc
     4621 tgaagcaacc gctcgtgggg ctgctgttac acagttacaa aatgttgatg ctcaacaagc
     4681 acagttgatt tctgctgtca cagcaaaagc tgataatgct ctttcaggct tggaagaaga
     4741 aaaaacagca cgagcaaacg ccgataaagc ggagtctcaa gcacgtgaag cattaacagc
     4801 aagaatggga gcagctgaaa gcaatatcgt aactattcaa cgtacggtag caaataatgc
     4861 ccaaagcatt agtgaagtca gtcagaattt aaatgctaag attgataata ttaatatcgg
     4921 cggtcggaat ttattaagag acagcgagtt caatgcgtac aataaatggg gtagcccaca
     4981 aattgaattt gctgaaaatg caaatcgccg aactatcaaa gtaacctcaa caggtactaa
     5041 cggtcctgtt ggtattgttt catctaatcg ccattccaca tcttatttcc aacaaggaga
     5101 aacttataca ctctcattat ttgctcgagg aagtaaggca ctagactatc tctatctaat
     5161 gcgtcaagat gggaataacg ttagattacc agtgattaac gttgcctctg aaactgagtt
     5221 taatcattac aaactcacgt tcaaggctcc ttttaccacg caacaaggtt atgtcttaat
     5281 cggttttcgg caaaccagca cagagcagtt tgttgagttt cacagcgtca aacttgaaaa
     5341 aggcaacgta gccacagact ggacaccagc accggaggat gtggagagtg cggttagtgc
     5401 tgtttcggct gatttaacga gttataaaca aactcaagcg gctaccgatt cagcacaagc
     5461 acagcaactt aatcagttat ctgtgaactt gaccaaagca gagactaatt tcaacgccaa
     5521 aatcacggaa gaaaaaaaag ctcgtgttga tgcagataaa gtgaatgcag ataaactcac
     5581 agacataaca agccgtgttg caaatgcgga atcaactatt accaatttcc aatcaaccaa
     5641 agcgaataaa agtgaggtgg caagtattgc ccagcagaac ttacagagca tttggcgcac
     5701 cgatgcgcaa agtgcggtgg atgccttgaa aatcggtggt gcaaatctac tggttgatag
     5761 tgagtatttg acgacagccc gttggggcgg tagctcacga gtggcaagct cgcaatatgg
     5821 tgatcgtcgc ttgactcaag tgtttgtgac ccaagcaggc acagggcatt ttggtgtgac
     5881 tcaaggcaca caaaaagcca ctacgcgtat tcgtcagggt gaaacctata ccttgtcact
     5941 aaatgcgcag gggacggcag gttttacccg cacagggtta aactatgtgt accttatccg
     6001 cgaagatggt ggcaatttta gattgccaac tttgccgctt actgcatcat tgtcccaacg
     6061 ccctaaagtg acatttactg cgccgtggac gagcaatcaa gttcggttac tgattggcgc
     6121 aaatggcata tttgaggcga cagactggtt cgcatttcac agcgttaagc tcgaaatggg
     6181 taatgttgcc acaggctgga caccaaccgc caaggatatt gacgacaaag tatcagcggt
     6241 tcagtccaac ctcaccgctt atcaggcggc acaagctaaa gcagatcaag ccaaagctac
     6301 gcagatttcg gggctcacaa cccgaatggg ggcagcggaa agtaacctca ctcgcaccga
     6361 acgggcagtg acggaactta atcagacaac ggtgacgaca ctgcgtgatc taaccgcgcg
     6421 caccaaaaca acggaaggtt cgctctctcg tttggaaacc gccaaagcaa ataaaactga
     6481 ggtggcaagt attgctcaaa gctccttaca gtca
//
LOCUS       DANLUA010000141         6510 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLUA010000141 DANLUA010000000
VERSION     DANLUA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..841
                     /locus_tag="PXB44_001995"
     CDS             <1..841
                     /locus_tag="PXB44_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282444.1"
                     /translation="FSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGKADKLVGVDT
                     TSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQIKASGIPLLH
                     LGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHSTDKEVLVLI
                     DTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADVILLASRNQA
                     KESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYAHQILTTIQP
                     CLK"
     gene            852..1838
                     /locus_tag="PXB44_001996"
     CDS             852..1838
                     /locus_tag="PXB44_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5282445.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            1838..2644
                     /locus_tag="PXB44_001997"
     CDS             1838..2644
                     /locus_tag="PXB44_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282446.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(2665..3303)
                     /gene="purN"
                     /locus_tag="PXB44_001998"
     CDS             complement(2665..3303)
                     /gene="purN"
                     /locus_tag="PXB44_001998"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL5282447.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(3410..5567)
                     /locus_tag="PXB44_001999"
                     /pseudo
     CDS             complement(3410..5567)
                     /locus_tag="PXB44_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(5621..6457)
                     /locus_tag="PXB44_002000"
     CDS             complement(5621..6457)
                     /locus_tag="PXB44_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL5282448.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
BASE COUNT         1915 a         1407 c         1228 g         1960 t
ORIGIN      
        1 tttttctttc attgccctat caatgtgttt aaataccaca aaggcggcag aaaaaattgt
       61 ttcaactgcg ggctatgctt ccgaaattgt cgcagcacta ggaaaagctg ataagttagt
      121 cggtgtggat accacttcag ttaaacctca aacgataatg gagaaaaaac ctaaaatcgg
      181 ttatcgtcgc cagctttctg ctgaaggtat tctctcttta accccagatt tgattatttt
      241 agcccctgat gccggcccta acgcagttat tgagcaaatt aaagccagcg gtattccatt
      301 acttcactta ggagagaaac cgagcttagc agggattcgt gaggatattc agcaaattgc
      361 caaggcgatt ggtgcagaat cagaggcaaa acaattaaca gaaaccattc tggcagatga
      421 aaaaaaatta gccgacattc gtagccaaca ttctactgac aaagaagtct tagtgctgat
      481 tgacactgca tcgcaaggta tctttgggct agggaaaaag agtgtgggtg atcatttctt
      541 aaccttgtta aatctaaaca atagtttcaa tgctgagggc aataagccac tcgccaatga
      601 ggctttagct gcaagcaatg ccgatgttat tttattagcc tcccgtaatc aggcaaaaga
      661 atctgcaacg atcagtaaat tagcagaatc cagcccacaa tacgcccaac tcacaaatac
      721 acaagcgggc aaaaaaggct gtgtttttgc aatcaatatt ttagatgcct tgggctttgg
      781 gccttacact gctcaatatg cacaccaaat tctcaccact attcaacctt gtttaaaata
      841 acggatacgc tttgaaactg catttaacct taattttcac tttaattatc agcttagtga
      901 tttgtaccca tttaggggcg atgtcattac ccctatccgc ttggcactgc aacaacgctg
      961 aatgcgaaag tgcagcctat attttatggc aaatccgcct gcctcgatta ctaggggcat
     1021 tattcatcgg ggcagccctc gctattagcg gagcaacaat gcaagggctg tttcgcaatc
     1081 cgttagtaga tccggggatt attggtgtct cttcaggtgc tgcattggct gcggcattat
     1141 atattgtggt gattcagcca ctactgccgg aatcaatcag ctattatgct ctacctattg
     1201 ccgccttttt agggggctgg ctggtgactt ttatgctcta tttactttct caacgtaacg
     1261 ggcaactaca tatcgccatt atgctattaa ttggtattgc tctagctgca tttagtggcg
     1321 ctttaaccgg catactgatt tatgttgccg atgataacca acttcgtagt ttaacctttt
     1381 ggagtatggg atctttagca ggtatgaatt ggcaaatggt ggcaatgatg gctgcggttg
     1441 tcttcatttg tagcccactt atttggcggg aacaccgtgc gttaaacgcc ttaacattag
     1501 gtgaagaaga tgcacaatac attggttttg acattgcaaa agtcaaacgt cgtcttgtgt
     1561 tttacgttgc cttattaacc ggcacaagcg ttgcctgtgt tggggctatc ggctttgtcg
     1621 ggctggttat tccacattta atgcgtttac tgggaggcag taaccaccgc catttattac
     1681 tcaatagttt ggtttgcggt gcaatcttat taatgattgc agatactctt gccagaaccc
     1741 tagccgcccc ggcagaaatc ccgattggta tttttaccgc cttatttggt gcgccattcc
     1801 ttgcagtaat ggtatggaaa agtgggaaac atatctaatg agcaatttat taacttgcca
     1861 aaatttaagc atcgttcatc aacaaaaaaa gttgctggag attcaagaaa ttgcctttga
     1921 acaaggaaaa tttaccgcca tcatcggtcc aaacggagcc ggcaaaacaa ccttgctcaa
     1981 agccttaagc ggtaataatg cacaaactaa cagtgccgta ttttatcaac atcaacgctt
     2041 aacagactta aacggatatg ctctggctca aaaacgggca atgctcagcc aacagagcca
     2101 aattgccttt cccttaaaag tagaagaatt ggtacaactc ggaagagagc catatcgcca
     2161 aagtgaattt gcaaaattta atgaaaaaat aaccgcttgg gctatggaaa caatgtcttt
     2221 aaccgcatta aaagaaagaa gtagcataca actttccggt ggagaacaac atcgagcgca
     2281 tattgctcga gtgctggctc aactcgttcc tgaaccgaat gccgatttaa gcggaagctg
     2341 gcttttgctt gatgaaccca ccaaccattt ggatattcat caccaatatc agcttttttc
     2401 cttactacaa aagctgaaac aacaaggctt aactatcatt gcgattttgc acgatcctgc
     2461 attggcaata aatcaagccg atcatatctt aatgctaaaa aatggagaaa aatttgctga
     2521 aatgtccgct aaagccattg cagaaagttg tttattagat gagctgtatg aaatgaaaat
     2581 gggctgtcgc tattgccctc acagcgggca atattatctt tgccctgcat tagctgaaag
     2641 ttagggtgcg atgcacccta tcgtttaatc ttctgcatat ccacaagcag gcagtagttt
     2701 accgtctaaa tacgctttgc cgtgctgaga aactaatcta cccgaacaga accactcaat
     2761 caccaacgga taatatcgat gttcttgctc aaccactcga gccattacat cttcaatctc
     2821 atcttcagga taaatcggca ctttagcttg taataccacc gcaccagcat ccacttcttc
     2881 attcacgaaa tgaatagtcg tgccgtgttc actttcccca gcctctatcg ctctttgata
     2941 agtgtttagc cctgggtatt taggtaataa ggaagggtga atattgagta ttttgccagc
     3001 aaaatgttgc gtaaattcag gcgttaaaat tttcatatag ccggctaata caattaactc
     3061 agctcctaac tgctcaattt gttcggcaat cgcaagatcc atttccacat tgctttggta
     3121 atctttacga gaaaatacaa atgtcgggat tcccgcctgt ttagctcgaa tcagcccata
     3181 ggcatctgcc ttattgcaaa ttacaccgca aatttgaccg cttgtatccg cagatttttg
     3241 cgcatcaatc attgcttgta ggttagaacc attccctgaa attaaaacca caaatttttt
     3301 catattacac accggctgaa tattcatcgt tctggatatt atgccttgaa tctcaacatc
     3361 agtacaggaa agaaaaaggg cgtattggtt aaaataagcc actttcaagc tagaaacgca
     3421 cctctgcccc tagtgtgtaa gttcgacctc tggcactatt tgtgaaacga taggtgtctg
     3481 tatcatagtc atactcttct gcggtggaag agttatacgc atttaaggca tcaagataat
     3541 ttcggttcat cacattttgt accccggctt ttaaggttaa atgtttattc actttgtaat
     3601 ttgcatataa atcgataatc accggaatat tcggtaactg ctcgtgaata atgttgccat
     3661 cttcgtctaa atcaataccg acacgtttcg ctttgcctgt atatttaaaa atgctagaca
     3721 gtgttaattt tttatccagc aatttagccc ctagctccaa agtcgcataa tcttttggta
     3781 gctgggtata agcttcagtg ccaaatccat aaagaatagt cgcacctttt gaagtgggtg
     3841 ctgtgctttc agttcgggta taactaccat tgacaaaaaa agcatcggaa tcgtaagcca
     3901 gctcaacttc ccaacctcga tttttaacct tttcagggtg attcacataa atttggaact
     3961 gtatagcatt ggtagcatca cttgaatttc gacatactgt tggcccacaa ccatacaacg
     4021 agaggttata caaatagtct ttaatgcgtg agttaaaata taccgctttt agccccaaga
     4081 tatcattatt gagtaataca ttatcttgat gaatattaaa cccaatctca taggttttcg
     4141 cactttctgg tttcaaaaac gggttcacac tcaagccatt tcgactggta ttaaacattt
     4201 cttgcacatt aggcgaacgg gaggtttgtt tgtaactgac aaaaggttga aaccaatcag
     4261 caacctgaaa tgctaaagtg gcagaaggat tcaacttata atgtttttta ttaacatcaa
     4321 acccaaaaga agggaaacaa taatccatat catcgctatc gcattctggc ttatatcctt
     4381 ttaactgata gcgtgaatag tgaagacttg aatttaaagt gacaatgccg tatttccatt
     4441 cggtgtctag gtaaagggaa tcaatatttt gcttacctgc cggcccaaaa gcggcattgc
     4501 taatcgctcc atcattattc ttactttccg gtaaatcacg ggaatattta tttccctgac
     4561 tcaaaacggc tggtgttatt caaatcaaac atccacattt tgtttttggt tgaggcatct
     4621 tctaatgtcc atagttttgc atcatcgtgg aaatcttgct tagtttctgt tactgctgtt
     4681 aggaatttca gatcaatcca atcagattga ggctggtaac tggattctaa ggaataggtc
     4741 acactatcaa tattacgccc cactaagcta tttttatatt gacgtccgga caatttaaac
     4801 tctgaatctt gatttggctt aaaggcaatt tttcccatat aagattcagg gtgatttttt
     4861 aacccttgca tataagcaga atccgttgcc attgtgccat tacctcggcg ataattttga
     4921 ctgatactac ttttgctaaa agccaataaa ctgccaagag aaccactttc cagcacatca
     4981 acttttccgg ctagtgcaag cattccgttt tttcccagat cattattgcc ataattaaaa
     5041 cggcttaata cgccgacttt atgttgttct agtagaatat cttcgacatc aatcgtttta
     5101 aaattagcac taccggctaa agcattcaat ccggctgcac cattgttgta gcctttcgca
     5161 atatcaactc gggttaaaaa attttgatca attaaagtgc tgaattggct cattggcccg
     5221 ttaccatcag tgtggaactt accacctccg tttgctgatg taccaagctg tgtttgtggc
     5281 acgccatcaa ctaaggtatt tacccgacca agaccactca tattacgaat attcacactc
     5341 acgccccctt gccccgggtc aatttgggta tagccacctg cgacagaacg gacaatacta
     5401 tctaaacttt gcattgcatt cacattcatt gctcgagaac tcaccgagcc ggctttactg
     5461 aaattctcaa aattagggtt ttccacagtg ccataaaccg tcacagtatc aagctccgca
     5521 atatttttcg ccttttcctc tgccaatgca gtttgcgatc ctcccataca caaaaccgga
     5581 aacaaaatgc gtgtgattat attaaatttc ataattctcc ttattgagtg acccaaagat
     5641 aatctgcctg tgaagaagta atttcttctc tctctgtttt aagcattagc accgctactt
     5701 tttttgttcg gtcatattcc gcaatatgca acggaacgcc aagatctttg cgaacgtggt
     5761 ggttacctgc aatcagcaag ctagtcgata acgcattttt tactaatttt tctgccattc
     5821 tgcgatcacg gaattgttgt actgccacca tattttccat cgggtaaggc attccgtgat
     5881 tcattgacag caacagttgt gcgatctttt gcttaacttc agaggaagtg gatacagaac
     5941 cttttaatgg ctccgcccca tttaataaaa tttcaacctc tttatctgtt aagttagtgg
     6001 ctataacacg actatttgaa cctaagctct ctaccactaa atcttgatac atcttccaat
     6061 cccatttttg ccattgaatt gcctctctta aatccgtggc tgataaatta accggtcggt
     6121 taaactgaat gcgatcaatc gcaggctgct gatccacatt taacatctcc aacgcaatta
     6181 aatgtaactt ggctttcttc gtaagttgat taaataaata caattcaatt tggtggtgaa
     6241 ttaactgagt atgctcctca ccaactaaaa cgtaatcttg agaagataga cgggaaagta
     6301 gttcagtaaa tgaaatcgtt tgattggtat tcagatcaat aatttggccg atagagtcca
     6361 cgttttgttc attaagggat tgcgttgaaa ccgagcagcc taaaagaaaa aaagccataa
     6421 aaaggctagc taaaaaagag gaaatacgag gtaacataac aaaaaataaa ttaatgagaa
     6481 ttattatcat tatgttatgt aaaaaaagaa
//
LOCUS       DANLUA010000142         6490 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLUA010000142 DANLUA010000000
VERSION     DANLUA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(110..185)
                     /locus_tag="PXB44_002001"
     tRNA            complement(110..185)
                     /locus_tag="PXB44_002001"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Asn,seq:gtt)
     gene            complement(301..1050)
                     /gene="moeB"
                     /locus_tag="PXB44_002002"
     CDS             complement(301..1050)
                     /gene="moeB"
                     /locus_tag="PXB44_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL5282449.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(1149..2378)
                     /gene="moeA"
                     /locus_tag="PXB44_002003"
     CDS             complement(1149..2378)
                     /gene="moeA"
                     /locus_tag="PXB44_002003"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL5282450.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(2400..3017)
                     /locus_tag="PXB44_002004"
     CDS             complement(2400..3017)
                     /locus_tag="PXB44_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL5282451.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(3085..4296)
                     /locus_tag="PXB44_002005"
     CDS             complement(3085..4296)
                     /locus_tag="PXB44_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL5282452.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
     gene            4493..6424
                     /locus_tag="PXB44_002006"
     CDS             4493..6424
                     /locus_tag="PXB44_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282453.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDESTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT         1998 a         1464 c         1265 g         1763 t
ORIGIN      
        1 tttgtgatta cggctatgca aaatgatttg caaaactatg caaaataaat cgaccgctta
       61 cattataaaa ggagacaaat aaaacaagaa caaaagaaaa ataaaatctt ggctcctcct
      121 gcgggactcg aacctgcgac atatggatta acagtccacc gttctaccga ctgaactaag
      181 gaggaattaa cggtgtaaat aataggctaa aacgttttag ccgtcaaata aaaaaaataa
      241 actgtcgttc attttttaca tttcttgaca ttttttcgcc aataagtatg agaaaaatac
      301 ttaataagag gtacaaaccg gacagttatt ttgcttaggt aatgccattt ctctgacaga
      361 aaaatgcaag ccgtcaatca ttaacaattt tccggaaaga gttctgccga tattaagtag
      421 cactttaatt gcttccaacg cttgaatact gcctatgatg ccaacaatcg gagcaatgac
      481 ccccgcttca acacaactta aagtcgcatc accaaacaac ctgcttaaac attcatagca
      541 cggttcttgt tcttgataac aaaatacact cacctgccct tcaaatcgaa ttgccgaacc
      601 ggaaacaagc ggtcgttttt gcttaaaaca ttgcaaattt aactgattac gcacagtaac
      661 gttatcggta caatccagca ctaaatctac atttgcaatc agcttggcta actgctcatc
      721 ggaacatcgc tgattgatag tttcaatctg aatgttcgga ttaatcgccg ctaatcgagc
      781 tttggcagat tccacctttg gctgcccgat agtcgcatcg gtatgtaaaa tttggcgttg
      841 cagattagaa agtgagacca gatcaaaatc caccaaagtc aaatgcccaa cgccggcaga
      901 agctaagtat tgcgatgccg cacagcccaa accacctaaa ccgacaatca acactcggct
      961 ggcttttaac ttttcctgcc cgtcaaaatc cacgcttttt aggataatct ggcgattata
     1021 acgtagcatt tcgtgatcgg taagttccat ttttacacct aaagagtaaa cgatggcgca
     1081 agcgtccacg cttgtgccta tatttttatt gctagcacaa gcgtggacgc ttgcgctatc
     1141 ttaattcatt aatacatcag cgaattaaac ggctgaatag tgactttttc cccagcatca
     1201 acattgcctc gttctgcttc tagcacaata aaacagttac tttcattaaa agcgctgaaa
     1261 atatgtgagc cttgcgtgcc aactgcacga acttctaact cacctttttc attggcatag
     1321 taatagcccc gttggaaatc ttggcgaccg accgcttttt tcaattttcc ggcactgcaa
     1381 gcggttagat tttgggttaa ttttgcaatt ttatctgtcg ataaacccga aagtttggct
     1441 aacgccggtt gcgctaattg gtagaaagtc actaatgcag agacagggtt gcccggcagc
     1501 ccaaaaaacc aagcctgttc taatttaccg aatgcaaaag gcttgcccgg cttcatcgca
     1561 attttccaga aaccgatttc gcctaatttt tccagtacat ctttggtaaa atctgcctct
     1621 cccacagata cgccgccact ggtaatcaac acatcggctt gctgttgggc ttgcctgaaa
     1681 gtttgctcaa acagaattgg gtcatcaggc aaaatcccaa aatctaaaat ctcgcagttg
     1741 agtttttcca gcattaaacg caccgcaaaa cggttggtgt cgtaaatttt tccctcactc
     1801 aaaggctcgc caactgaggt taattcatcg ccggttgaaa gaattgccac ttttaatttt
     1861 acaaaaacag gcacagaagc aataccgagt gaagctagca acggtaaact ggctacattc
     1921 aataatgagc cttttttcaa gaccaatgcc ccctgtttaa catcttcccc aatgcggcga
     1981 atgttactgc ctaattttgg aatatggtta aaggttacag agccatcgga atttaacgta
     2041 gtttcctctt gcattacaac ggcatcaacg ccctttggca ctaatgctcc cgtcataatc
     2101 cgcacgcatt cgcctgcctt tatttcgccg ttaaacgggt taccggcaaa ggctttacca
     2161 gccacattca aggttaaatt ttgtgctaaa tctgaaatac gaaccgcgta gccgtccatc
     2221 gccgaattat caaaattcgg tacattaatc ggggaaaaaa tatcctctgc caacactcgg
     2281 ttagcacact catttaacgg aagttgttcc accaatttag gtgtcgataa acgagcaagc
     2341 atattatcta acgcatcact caaagccaaa agtgccataa tttcctacct attcaacgtt
     2401 tagctaattt gtacaaagaa atcccgtacg ccaatcaaca gattattcac cgcaatcgca
     2461 gccagaatta accccatcac tcggctgata atcgcagccc ctgcattacc aataaggtgc
     2521 tgaatacggt ttgccgctaa tagcaacaaa taggtaataa agagtaccaa aagcataatg
     2581 ccggcggtga taatttgatc gccgatatga aaacggtgat tgtcggttaa taatacgatt
     2641 gccatcatcg ccccgggaga agcaatcgac ggtacagcta gcggataaac cgccaattca
     2701 ttcagattac tgctgatccg catttcttgc tcgggcttgc tttcgccaaa aatcatcgtt
     2761 aaagcaaata acaacagcac taagccgcct gccgcttgga atgcaggtaa cggaatctgc
     2821 attgattcta acagcacttg cccaccgata aggaagaaaa tcaaaatacc ggcggcaatc
     2881 aagaccgcat ttagtgcaat tttacgccgt gcttcaatcg gcaaaccaat ggtttttgcc
     2941 aaataaacag gaacagaacc aatcgggtcg atcaccgccc ataaaaccac aaattgtacc
     3001 gctaatgaat caaacatatt ctctcctgtt atcttgataa tttgcaaaat tttagcataa
     3061 aacaaccgct tgcacggctt tttcttacag atagctcacc ggtaacgcta ccaaattttc
     3121 acttaaataa agtttggtcg gatattggtt aataatcgct ccctgcccta atttccattc
     3181 actagaaact gagtttagca agtcaaaact ttttgccatt tttttggttg ggcttgccgt
     3241 gaccttaatt tctatcggat aaagcgtgcg atcatattca ataattaagt cgatctcttt
     3301 ctggttagta tcacggtaaa aataaatcgg cggctcaatt ccttgattat gaaaggattt
     3361 tataatttgg cttaccacaa aggtttcaaa gaactgccag ctttttgccc cttgagcaat
     3421 agtttcgggg gtaagccatt tggttaaata cgccattaac ccggtgtcta gaaaatacac
     3481 ttttggggtc ttaattgccc gtttcaaatg attgttgtgg tatggctgga gcaaatagat
     3541 aatgccggaa gtctgcaaaa tggcgaccca acgtttaatc gtttcattcg atactccaac
     3601 ttcttgagca atattactgt aattgagtag ctcaccacta cgagaggcaa tagagaccat
     3661 aaaacgggta aaatccgttg tgctggcaat attggttaat tggcgaacat ctcgctcaat
     3721 gtaggtcgct acataagagc gataataaat ctcccaatcc acctcttgtg catataaacg
     3781 aggcatatcg cctttatgga taagctgcca gatgttctcc ggtgatttta attcttgctc
     3841 ccgagctaac aaatagtcgg ttgtcggtaa aaaaggttga tcgaacttca cccctaaaat
     3901 ttcacgcaac gaaagccctt gtaattttaa aatagcaata cgaccggcaa gtgtttcact
     3961 cacattctgc attaattcaa aggcttgcga ccccgaaagt aaaaacaaac cgttttgctg
     4021 ttttttatca atttctaact ttaataacgc aaaaatttca ggcacatact gtacttcgtc
     4081 taaaataagc ttgccttgat ggtttaaaaa aaacaggcga gattcttgtt tagccagctc
     4141 cagcaacatt gggtcatcaa aagtaatata ttgatagccc tcaccaatat gttgtaacaa
     4201 tgtcgattta ccaacctgtc ttgcccctgt cactaaaacg gcaggaaatt gcgccacgag
     4261 tcgttttaac agcgattgca tttgccgaga aatcattttt cctccaaaat agcctaaatc
     4321 aagtttaacc tcatatataa tgttgaatta aacttaattt aaattatttt ttaagtttaa
     4381 ttcaacacag aatattgaat taaactgaat ttcttatttt agttcacaaa gcaatggctt
     4441 tgctttttta tgctaaaata cgcaaaattt ttaacaaaat agaccgcttg caatgatctt
     4501 ttttacagac ttaattttaa aacgagggca ttccgttctc attgataaca gctccgttac
     4561 cattcacacc gggcagaaag tcggtttggt gggaaaaaac ggctgtggca aatcctctct
     4621 ttttgcctta ctgaaaaatg aattacaacc tgaaggtggc gatgtatcgt tgccgaaaaa
     4681 ctgggcgatc tcgtgggtaa atcaagaaac gcctgcgtta gctatttcag cgttagacta
     4741 tgtgattcaa ggagatcggg aatataccca tcttatggct gagctagata tcgcaaatga
     4801 aaagaatgac ggcaaccgca ttgcgattat ccatactcaa cttgatacca ttgatgcttg
     4861 gacaattcaa tcccgtgccg ctaccctgct taacggcttg gggtttagca ccgaacaact
     4921 gcaactgccg gtgaaatcct tctccggtgg ctggcgtatg cggttaaatt tggcacaagc
     4981 tctgatttgt cgttccgatc tgttattgct tgacgagcca accaaccact tagacttaga
     5041 tgcggttatt tggttggagc gttggctgag taactatcgt ggcactttat tgttaatttc
     5101 gcacgaccgt gattttctcg atccgattat cgatcgggtg attcatattg aacatcaaaa
     5161 gctcaatgaa tacaccggca attacacctc gtttgagatt caacgtgcga ccaaaatcgc
     5221 ccagcagaat gccgcttatc agcaacagca acgtaaagtt gctcatttgc agagttttat
     5281 cgatcgcttt aaagccaaag cgactaaagc gaaacaggca caaagccgaa ttaaagcgtt
     5341 ggaaaaaatg gagcttattg ccccagccta tgctgattcg ccgtttgaat ttgaatttcg
     5401 cccaccgctt gcactgccta gcccgttatt aatgatggaa aaagtcagtg ccggctatgc
     5461 ggaaaaaacg gtgttggaat cggtcaaact gaatttggtt cccggctctc gtattggttt
     5521 actcgggaga aacggtgcag ggaaatccac gctgattaaa ttattggcgg gcgaacttcc
     5581 gccccaaaac ggctcaattc aactggcaaa aggcgtgcaa ctcggctatt tcgcccaaca
     5641 tcaggtggat acactacgtt ttgatgaaag cccactttgg catttacaaa aactcgcacc
     5701 ggaaaaaacc gagcaggaat tacgcaatta tttaggcggt ttcgatttta aaggcgataa
     5761 agtggtacaa gctgtgaaat cattctccgg cggagaaaag gcccgtttag tgcttgcctt
     5821 aattgtatgg caacgcccga atttactgct attagatgag tcaaccaacc atttagattt
     5881 agaaatgcga caagccctgg ttgatgcact gacctattac gaaggctcgc tagtggtggt
     5941 ttcgcacgac cgccatttat tgcgtagcac cgtgaatgaa ttttatttag tgcacgaccg
     6001 caaagtggaa aaatttaaag gtgatttaga cgattaccaa aaatggctca atgagcaaaa
     6061 cgccttagcc aacgagccga aatccgtact tgcaaaagat tcggcaaatt cgaccgcttg
     6121 ctctagcacc aatgaaaata gtgcagctca acgcaaagag caaaaacgct tagaggcaga
     6181 gcgccgccaa caaaccgccc cattgcggaa aaaaattact caactggaaa aagagttaga
     6241 aaaagcaacc gaaaaattaa ataaactcga agcaatgctt accgaaagcg acatttatga
     6301 agccgaaaat aaagcaaaac taactgaaac acttgcgaaa cagttagagg caaaaaggct
     6361 ggttgaagaa attgaaatgc aatggttgga ggctcaggag gagtttgaga ccatgttacg
     6421 ctagggttct acaagatagc gcaagcgtcc acgcttgtgc catttaaggg aactatacac
     6481 ctcaaaaaaa
//
LOCUS       DANLUA010000143         6482 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLUA010000143 DANLUA010000000
VERSION     DANLUA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(38..346)
                     /locus_tag="PXB44_002007"
     CDS             complement(38..346)
                     /locus_tag="PXB44_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5282454.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            complement(348..716)
                     /locus_tag="PXB44_002008"
     CDS             complement(348..716)
                     /locus_tag="PXB44_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282455.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            complement(691..1797)
                     /locus_tag="PXB44_002009"
     CDS             complement(691..1797)
                     /locus_tag="PXB44_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL5282456.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(2037..3080)
                     /locus_tag="PXB44_002010"
     CDS             complement(2037..3080)
                     /locus_tag="PXB44_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5282457.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(3184..3468)
                     /locus_tag="PXB44_002011"
     CDS             complement(3184..3468)
                     /locus_tag="PXB44_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282458.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(3498..3683)
                     /locus_tag="PXB44_002012"
     CDS             complement(3498..3683)
                     /locus_tag="PXB44_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5282459.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(3680..4246)
                     /locus_tag="PXB44_002013"
     CDS             complement(3680..4246)
                     /locus_tag="PXB44_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5282460.1"
                     /translation="MKSLSSTQIEEIRKQMVKFATLQLADKDLAEDVVQDALTNAYKH
                     SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEH
                     QDVTEWRGVAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISL
                     SNLNVLLHRARLRLQECLSRNWFREESK"
     gene            4355..5308
                     /gene="msrP"
                     /locus_tag="PXB44_002014"
     CDS             4355..5308
                     /gene="msrP"
                     /locus_tag="PXB44_002014"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL5282461.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            5308..5892
                     /locus_tag="PXB44_002015"
     CDS             5308..5892
                     /locus_tag="PXB44_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL5282462.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
     gene            5989..>6482
                     /locus_tag="PXB44_002016"
     CDS             5989..>6482
                     /locus_tag="PXB44_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL5282463.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVTARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEADESYFGGARKGKRGCGAAGKTAVFGLLKRDDKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAENHNHIN
                     RIEN"
BASE COUNT         1926 a         1368 c         1246 g         1942 t
ORIGIN      
        1 ttaaaaaagc tcaaacaata atgtttgagc tttggtttta ttcaatgact tttgcagtat
       61 atttgaggtt tccgaccgct tgtatcaggg cttggttatc cacactttct tttaccacca
      121 cagtcgcttt tcgctctttc attgaggctt tggtggaaat cacaccttct actgctcgta
      181 gttctttatt gactaaataa acgcagagct ggcagtacat ttccggaata tggaggacga
      241 cggttttctc tgcttgtttc gtttcaattt gagctgtttt ttcttgtgcg tagcttgaat
      301 gaaccaaacc aaaagccaac agcatcgttg cgatatattt tttcatttta ttctccgctc
      361 cacatttcca aaatgtaagg caatacataa gggtaagtta aaaagaacat caccaccaca
      421 gaaactaccc aatacaaaat gaccagggtt ctgcgggaga agtatttggt gcagatgatt
      481 ttgttggaaa aattcagcaa ccagaagcca tagccaaacg cagcgagcga aatcagcaac
      541 atcggcagtt gtagaaactc cagttggttt agctccatca accaagggga agagatgcca
      601 aacactaaat aaaccagtgg cccaatacaa cagaatgtgg aggtcaccgc cgacactatg
      661 gcggtcacgc acattgtgat aaatttatgg ttataactct ttagagatga attcatagcc
      721 aaattctttc gcctcaaacg agtttaacgg gtcaccacct acctcagcat aagggtagcc
      781 aaagttattc agtggtgtaa gttccggttg tggatataaa tcgaaatcac gtccgtgatt
      841 gaagcctacc cagttcattt cccagttgcc gaataagtat ttcgagaccg cttgaacatc
      901 agcatcatcc acgtcttttt tctcggctaa acgcatttta gcgacatcgg cagaatctac
      961 aggcacccag ccgaagcctg ctaaatagaa ctctgcacgg cagtgctgcc cgccgctttc
     1021 attcgccacg ccatcttttg agctaccaaa tgcggttttg gaatattgcc ccatttttac
     1081 cgcttgccct aaacgaatac caaacacttc acgagcagga atgttagagg cacgagcaag
     1141 tgctacaaac accgagttga tgtcggtaca tttacctttt agcactttgg tggtgagaat
     1201 tttctccaca tcgccatcgc cacagcctag caccgagtta tcacgttcca tattatcaac
     1261 aatccacttg tggataagtt ccgctttttt caacggattt tgctcgttac ccacaatttt
     1321 atcggcatat tctttcacaa taccgtctgt tttaatgtga gccgttggtt ttaaatactc
     1381 caatacatca acagaataag tgattttctc cggcatttgg taatttgcca acgccccttg
     1441 agccataggc tcacgatctt gggtttggat caccattttc acttttagat cacgctttgg
     1501 tgcatcagta tcccaagtgg caaacaaggt ttttgcacca taagcgttat tttctgtgat
     1561 ataggcgttt ttgtaacttc cctcaaactc tatcgatttt accgtttgat aatcggtatc
     1621 agacggcaac ggaatccaca aattagttgt tcctttcgag ccttgtggca caactaaatc
     1681 ataagattgc gtaaattcgt aagtgtgggt agtggtgtgg aaattgttct gttgaacagg
     1741 agcagcaaaa gccgtcgcac agcttgaagc caccaaagag gcaataagtt tattcattga
     1801 aatgatcctt attagagtga aaaaacagtt aaaacttaac atattaagta ttctaactac
     1861 ggattgaggt tgcatattag ccaatcgaga tgggaaaagc aatgggggag atgatttttt
     1921 agatagtgca agcgtccacg cttgtactag aaacaaagct aaaataatca ataggcacaa
     1981 gcgtggacgc ttgcgccatc agagagaata acaagcggtc aaaaatcctc aaaattttac
     2041 aaatagattt tttgccctaa ttttgcactt tcaaatacgc tttcgatcac ttttagcaca
     2101 aaaatgacgt cttcgagttt gataattggc tcagcattgt gagcgatggc ttggtacaaa
     2161 tcatcaaaca agttttgata actgacttcg gcatttcgat agggctggcg aaccacatta
     2221 ccttccactt cagtgtgtaa aattccccag ttctcctttt cttcgatatt ccaatctttg
     2281 ccaataggtt gtacgccatt tttcagcaaa tcttcttgat tatcgggagt ttgtttcaga
     2341 taagagccta attttccgtg taatacaaat gtcgggcttg gttcacgagc atatcggctg
     2401 gctttcagca ccactttaaa accgttagca taatagagct gaatatcaaa gttatcatca
     2461 accaatgccc cttcgtgctg ataacgcaca tcagcataca ccgcctccgg cttcccgaaa
     2521 agatacaaag cttggtcaat taaatgtacg cctaaatcat acactaaacc tgtaccaagc
     2581 tcgcctgatt ctttccatac tttaacattt ttctctttcg cataacggtc aaaacgaatt
     2641 tcgcaatcta ccaactctcc cagtaaattt tgctccatta gcgattttgc cgtcgcaata
     2701 tggctgtccc aacggcggtt ttggtacaca taaagcacct ttccttgccg ctttgcaagt
     2761 tccgccaact cttccgcctc agccactgtt gccactaacg gtttttcaac taaaacgtgt
     2821 ttgcctgcaa gcaatgactc ttttaccatt gcataatggg tttgattcgg tgtggtaatc
     2881 agcactaaat ccacttctgg cgttaaaaga gcgttgaaat cccgcactgt ttctgcattc
     2941 ggcaaccatt ctgccgcatt gttagaggtt cgttcatata cctttacaat attaaaacga
     3001 gcatcttgtt tcaaaaacgg gatatgaaaa actcggctgg aaaagccggc acctgcgatg
     3061 gcaatattga tgggttgcat agacaatcct cataaaattt tgatgttatc tgcttaaata
     3121 aacaagcggt caaattttag gaaaattttg caaaaaacag ataaaatacg accgcttgtt
     3181 taactaatca taggttttgc aaacaatatc tttttcttca caaaacattt cactgtggtt
     3241 tgcacggcat tgcttttgta catttaattt ggctctgcct cggctactat gttcactacg
     3301 atattcctga gtaaatggct ttagagtaca aatgtgaacc tctttggaat caagtggatc
     3361 tgagacacca attataatcg gcatagggat accggcttgc cccgtaggag taaccggcac
     3421 gacacagcca cttaatagta tgcttaaact tattaaccaa taacgcatat ttttctcctt
     3481 ttaaaatgta taataaatca gaacggagca tctttatcgt tacagtaagc tcttaatatc
     3541 ttgcctaatt tcttggtatt tttattaaaa tttcggcatt tagggcatac caaaagatgc
     3601 aactgcaaac gcatatgttg ttgcaaacga agtttctcat cacaagactg tgaaataagt
     3661 tctgttgctt gcctgcattt cattttgatt cctctctaaa ccaattgcga gataaacact
     3721 cttgtaaacg taaacgagca cgatgaagta atacatttaa gttagagaga gaaatttcac
     3781 attcttggca aatttccgca ctactcatct ccaaatgttc acgcatcata aatactcgtg
     3841 cttgctgggc gggtaattta tttaaacaaa tatcaaaaat tgcccaaaat tctttttggt
     3901 aagttgattg agctaccccc ctccattcag ttacatcttg atgttccaac caatgatcgt
     3961 ggcgatcgaa aaaggcactt ggtgattctt cttcctcaca tagagaactg actggaatat
     4021 gtcgttttcg tgttctgagt aaatctaaaa ttttgttctt taaaatagca aaaatccaag
     4081 ttttaagtgc agcttctcgc cgaaaagagg ctgaatgctt ataggcattg gttaaggcat
     4141 cctgtacaac atcctcagct aaatctttat cggctagttg tagggtggca aatttaacca
     4201 tttgctttct aatctcttct atctgtgtag aagatagact cttcatatat ctcctttagg
     4261 gaatacccaa taaaaaatta cacttatttt gtaagaattc tagatcagct gagtccaaaa
     4321 taataacaac aattcataaa aaaaggatag ttcgatgaaa aaattaacaa ttcaagatgt
     4381 tacacccgaa actattttct accaacgccg taaaattatt accgcgttag gtttaggtat
     4441 tgcagcttct gcattgccta aagtaagtat ggcagaaaca caaagacact tgcaatcact
     4501 acagtttaaa ccggatccaa ataaagggct tgaactcact ccggaaaaca aagtaatagg
     4561 ctataacaat ttctatgagt ttggcgtgga taaaagtgca ccagcaaaaa atgcggcaaa
     4621 cttcaaaacc gatccttgga cattggaaat tggcggagaa gttgaaaatc cgtttagctt
     4681 aaatcatcaa caattgctta atacgtttcc actagaagaa cgcatttacc gttttcgctg
     4741 tgttgaagcg tggtcaatgg ttgtgccgtg gatcggcttt gagttagctc gcttaattga
     4801 gatggcaaaa ccaaccagta aagccaaata tgtggtcttt cacactttat atgatcctgc
     4861 acaaatgccg gggcaaaaaa atcgcttgct aggcggtggg attgattatc cttatgtaga
     4921 agctttacgc ttagatgaag ccttaaattc attaacacta ctatccgtcg gtttatacgg
     4981 taaaaccctg ccaccgcaaa acggtgctcc tattcgctta gtcgtgcctt ggaaatatgg
     5041 ctttaaaagt attaagtcga ttgtgaaaat cacctttgct gaaaaacgcc cactcactac
     5101 ttgggaaagt cttgctccaa atgaatatgg cttttatgct aatgtgaatc caaatgttga
     5161 tcatccacgt tggtcgcaag catctgaacg agtaattggt gcaggcgggt tattatccgt
     5221 caaacgccaa ccaacactaa tgtttaatgg ctacgaaaat gaagtggcaa atttataccg
     5281 tggcttagat ttaaaggtga actactaatg ctaacctggt tacgtatact cattcatctt
     5341 ggctgtttat taccgctagt ttgggtagca cagctattat atacgggtaa tgaaacaata
     5401 ttaggtgccg atccgattaa agagctggag cattttctcg gttatggtgc aatcgtcatt
     5461 ttttgtataa tgtttctact tggtattgcc ctacaatacc taaagaaaaa ccagtatcaa
     5521 attttacgcc gtccattggg cttatgggcg tttgtatggg cagctctaca tatttcaagc
     5581 tatctattgt tagaattaag ccttgatgta aaattatttt ttagcgagct tgccggtcgc
     5641 ccatatttga ttttaggagc agctgctttt ttcattctaa gcataatggc aataacttcg
     5701 ctgcctatgc taaaacgaaa attaggaaca cgctggggga cgattcacca gtgggcttat
     5761 cctgcattag tattggcgat ggtacattat tattggtcgg taaagagcgt aacattcgat
     5821 ccaattatca tcggagtctt agtgctgttt attgttgctc ataaaataca ggctaaattt
     5881 gcaaaaaaat agcaaaatca caccgcttgc agcagtcaaa ctacatacaa aaaataaggg
     5941 gctgtcctag ataacgacta aaacatccat ttaggttaag ataacccaat gagaaaaagt
     6001 cgtctaagtc agtacaaaca acataaactc attgaactgt ttgtagcagg tgtcactgct
     6061 cgaacagcgg ctgagttggt gaatgtaaac aaaaccaccg ctgcctatta ctttcaccgt
     6121 ttgcgattgc tcatctatca acatagccct catctggaaa tgtttgaggg tgaaattgaa
     6181 gcggacgaaa gttatttcgg tggtgctcgc aaaggtaaac gagggtgtgg tgccgcaggc
     6241 aaaactgccg tatttgggct gttaaaacgt gatgacaagg tttataccgt tgtcgtacca
     6301 aatacgcaat ctgccacact tttgccgatt attcgggaga aagtgaagcc agacagcctt
     6361 gtttacgccg atttttatcg aagctatgat gtgcttgatg tgagtgaatt taaccatttt
     6421 cgtatcaatc acagcacaca cttcgctgaa aatcacaatc acattaacag aattgagaat
     6481 tt
//
LOCUS       DANLUA010000144         6307 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLUA010000144 DANLUA010000000
VERSION     DANLUA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            105..1331
                     /locus_tag="PXB44_002017"
     CDS             105..1331
                     /locus_tag="PXB44_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL5282464.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            1388..2764
                     /locus_tag="PXB44_002018"
     CDS             1388..2764
                     /locus_tag="PXB44_002018"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL5282465.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(2819..4408)
                     /gene="purH"
                     /locus_tag="PXB44_002019"
     CDS             complement(2819..4408)
                     /gene="purH"
                     /locus_tag="PXB44_002019"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL5282466.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            4580..4849
                     /locus_tag="PXB44_002020"
     CDS             4580..4849
                     /locus_tag="PXB44_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5282467.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            complement(4936..>6307)
                     /gene="dacB"
                     /locus_tag="PXB44_002021"
     CDS             complement(4936..>6307)
                     /gene="dacB"
                     /locus_tag="PXB44_002021"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL5282468.1"
                     /translation="QAEINTEELLNTLPAGTSVSFIAKNLDTNQIITQYQSDIFMLPA
                     STQKVFTALAAKLTLNDDFRFQTALLTNGKVENGVLKGNLIARFSGDPELTSGQIYQL
                     MSKLKQQGINKIEGDLILDPSVFASHDKASGWIWNDLTMCFNAPPAAINVDHNCFYVT
                     LNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPKEAPFCQLDVVVHDNNRYQIKGCMAR
                     QSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAFNGQVKEPLTAQNGTLLAEHYSEPLP
                     VLLKKMMKKSDNQIADALFRTVANKQHNRPASFQLGSYVIRQLLKTKANIDFKNSVVA
                     DGSGLSRHNQVSSRTMLETLEYIAQNEESLKLFETFPIAGVDGTISGRGSISTEPLAK
                     NLIAKTGSLKGVYNLAGFMKNARGERIAFVQFINGYSTGELESKTKRAPLNTFENKFY
                     MALFNE"
BASE COUNT         1743 a         1244 c         1350 g         1970 t
ORIGIN      
        1 gcttttaata gtttgatcaa aaaatacgta aaatcttgtt gtgttactgt ttcccatcgc
       61 taatatagca tagcttgtta gataattctt taaaggagat tactatggct atgccatctc
      121 aacgtttttc tcttacttgg gtactaaatt tatttggtac tgcggtagga gccggcgttt
      181 tatttttgcc aattaatgca ggtatgggtg ggttctaccc acttattatt atgactttgt
      241 tagttggacc aatgacatat ttagctcacc gtggcttaac acgttttgtg ctttcttcaa
      301 aatacaaagg gagcgatatt acggctgttg ttcgtgaaca ttttggtgaa caggccggta
      361 aattgatcac gctactgtac ttctttgcca tttttccgat tttactgatt tatggtgtag
      421 gtattaccaa taccgtgagt tcttttatgg agaatcagct tcatatcgcg ccaccatcta
      481 gggctgtatt atcgtttatg ctaatagctg ctatgatcgg agtaatgtta ttaagtgagc
      541 aagtcatgtt aaaaatcaca acttgtcttg tgtatccatt agtgttgatt ttattgggct
      601 tatctattta tttaattcca cagtggaata cagctgcctt acagcaaatg ccaactacag
      661 gagacttcct tactacactt tggctgacta ttcctgtttt agtctttgct tttaaccatt
      721 caccggctat ttcttctttt gcactttctc aacagaaata ttaccaagat gacaagaagg
      781 ctgaaattga atcagctaaa gtattacgtt ctaccgcctt tatcttggtg ttatttgtga
      841 tgttctttgt atttagctgt gtattaactt tgacacctga agaattagca caagctaaag
      901 tgcaaaatat ttcgattctt tcttaccttg cgaataaatt tgataatccg attatttcct
      961 attttggacc gttggttgct tttttggcga ttggtagctc tttctttggg cattatttag
     1021 gtgcgcgcga ggggttagaa ggattggtta atcaaatgcg taaagagccg attgatccaa
     1081 gcaaattccg caaaattacc gctattactt ttttgatcat tttatggatt gttgcgacta
     1141 tcaatccaag cattttaggc ttcattgaaa gccttggtgg gccgattatt gcgatgatct
     1201 tgtttattat gcctgtctat gccgtatata aagtaccggc acttgcacgt tttaaaggtg
     1261 aatttggcca tttatttgtg cttgttatgg gttgtattgc tatttcagca atagtatatg
     1321 ggttactgta atctttctaa aatcaatgta gaatacgccc ttttattttc tatgtgaatt
     1381 tgtgtttatg attagtgtat ttgatatgtt taaagtaggg attgggcctt ccagttccca
     1441 tactgcgggg ccgatgaaag cggcaaagga atttgtcgat gaattacaaa atcgcaaatt
     1501 tttaaacaaa attgaccgct tgcgggtaga tgtctatggt tcattatcac tcaccgggcg
     1561 aggacataat acggatattg ccatcataat gggattaatg gggtatttgc ctcataatgt
     1621 ggatattgaa cgtattgagc agacgattga aaatctaaaa caaaataagc aacttatcat
     1681 tgctgaaagc agtgttgagc ctaaagtgat tgcgttcgat ttttatgagg atatgccatt
     1741 tcatcgagat ttcttacctc ggcacgaaaa tggtgtgaaa tttagtgcct tgagcggtga
     1801 agagttgcta tttcaaaaca cctattattc tattggtggt ggctttattg ttgatgaagc
     1861 taactttgag caagttaaaa attcagatct ttctgttcca ttcccatatc agaatgcagc
     1921 agatttactt gcacattgta agaataacgg gttaccgctt tcttctttag tctggaaaaa
     1981 tgagcttgct ttacgcagta gagtagaaat ttcggattat ttggcaatga tttggaatac
     2041 aatggaagcc tgtattgagc gtggtttgaa tacggaaggg ttactacccg ggcctctgaa
     2101 agtggtacgc cgtgccagtg ggctacgcag aatgttagag gcaaacagta aattcaatac
     2161 cgacccaatg caaattatcg attgggtgaa tatgtttgcc cttgccgtga atgaagaaaa
     2221 tgcggcaggt gggcgagtag tcaccgcacc taccaatgga gcctgtggta ttgttccggc
     2281 tgtgttggcg tactaccaaa aatttattgc acagcttaat caagatacta ttgagcgtta
     2341 tttattgact accagtgtga ttggttcgtt atacaaaatg aatgcctcta tttccggtgc
     2401 tgaagtaggt tgccaagggg aagttggtgt tgcttgttca atggcggcag caggtttaac
     2461 ggaaattatg ggtggcagtg ctgaacaggt gtgtatggct gcagaaattg caatggagca
     2521 caatttaggc ctaacttgtg accctgttgg cggacaagtg caagtgcctt gtattgaaag
     2581 aaatgcaatc gcttcagtca aagcgattaa tgccagcaga atggcactac gccgtacttc
     2641 ttcgcctcag gtaagtttag ataaagtcat tgaaacaatg tacgaaaccg gcaaagatat
     2701 gaatgcaaaa taccgtgaaa cctcaaccgg tggcttagca atcaaaattt taccttgtga
     2761 ttaattaatg aaaaaggcta ccttatcaaa ataaggtagc cttttagttt gtagaacatt
     2821 aatgacggaa atgcctcatt ccggttagta ccactgccat accgtgttcg ttggcggcat
     2881 caatcacttc ttgatcacgc attgaaccac ctgggtgaat cacacaagta atccctactt
     2941 ttgcagcagc gtcaatacca tcacggaacg ggaagaacgc atcggaagcc attacacaac
     3001 ccacaacttg taagccctcg tcttccgctt taatacctgc aatttttgcg gaataaacac
     3061 ggctcatctg ccctgcaccg ataccgatag tttgcccgtc tttggcatac acaatcgcat
     3121 tcgatttcac atatttcgcc actttccagc agaacaatag gtcttttagc tcagcttcag
     3181 ttggtttacg ctctgaaacc acctttaaat cgtcaataga tacagagcct tggtcggcat
     3241 cttgcactaa taatccgccg tttacccgtt tgaaatctaa gcgttttaca gcttctgaga
     3301 actcgccaca ttcaagcaca cgcacgtttt tcttagtggc taacacttct ttggcagaag
     3361 cagaaacggt tggtgcaata atcacttcaa caaattgacg gtctgcgatg gtttgagcag
     3421 tttcgccgtc taattcacgg ttaaaggcaa taatgccacc gaaagcagaa gttggatcgg
     3481 tttggtaagc acggttgtag gcatctaaaa tatttttacc caaagcaaca ccacaagggt
     3541 tagcgtgttt tacaatcaca caagcaggct cgctaaattc tttcacgcat tcaagagccg
     3601 catcggtatc tgcaatatta ttatacgaaa gggctttacc ttgtaactgt ttggcggtgg
     3661 aaacagaggc ttcttttaac tcattttcga catagaatgc agcgttttga tgcgaatttt
     3721 cgccataacg catcgtttgt ttacgcacaa aattgaggtt taaggtgcga gggaattgtc
     3781 cacttggttg ggttaaatct tcttcctcag ccccttgata aggcggcact ttttgcccga
     3841 aatagttggc aatcattgaa tcgtattgtg cggtatgctc aaacgcttta atagcaagat
     3901 taaaacgggt ttctaaagtt aaactgtttt gatgttgatc catttcggca agaatagcat
     3961 caaaatcatt gttattgacc acaatcgcca catctttatg atttttcgct gcagaacgca
     4021 ccattgtcgg accaccgata tcaatatttt ccaccgcatc ttcaaggcta caatttggat
     4081 tggcaacggt ttgagcaaaa gggtagaggt ttactaccac catatcaatt cgttcaatgc
     4141 cgtgttggtt catcacttca tcatcagtac cacggcgacc tagaattccg ccgtgcactt
     4201 tcgggtgcag ggtttttaca cgcccgtcca tcatttcagg aaagccggtg taatctgata
     4261 cttctgttac tagcaaaccg gcttcagcca gtaattttgc ggttccgccc gtagaaagta
     4321 attttacccc acgagccgat agcccacgag caaattcgac aatgcctttt ttatcagaca
     4381 cacttaataa cgcctgttga atcgccattc ttaaattcct taaaatgagt gttaacgtga
     4441 aaataaacgg caaagattat aacgcaaacg tttgcttttt tcatctacaa gtggtcgatt
     4501 ttgcaaattt ttctgcaaaa tcgaccgctt gcactcgtga ttaaagcggg tagaatagag
     4561 gaaaaaaatc ggaggaacta tgattaacca caatttagca gatttcgtat ttgagtattt
     4621 taccgaaaac aacctaaaag caggcacact acgttatcgc tacataaaag agaacgtgat
     4681 tgatgcttac agtacccgag tagatgatgc ctttcaaggt aaaggtattg caggagagct
     4741 ttacaatgct ttaattgcgt ttgcgcaaga aaaagggctg aaaatcaagc caagttgtag
     4801 ttatattgag gtaaaaatgc aacgaagtca tcgagattta attgcttaga gaggagcaag
     4861 cggtcgtaaa atttcaaaaa tttacgaccg ctgtcttatt caattctcaa aaatccatca
     4921 cacaaaatat aagccctatt cattaaacaa cgccatataa aacttatttt caaatgtatt
     4981 aagcggagcc cgtttggttt tgctttcgag ctcgccggtg gaatagccgt taataaattg
     5041 cacaaaagcg atgcgttcgc cacgggcgtt tttcataaag ccggcaaggt tgtaaacccc
     5101 tttcagtgag ccggttttgg caatcagatt tttggcaagc ggttctgttg aaatagagcc
     5161 tctgccggaa atagtaccgt ccacgcctgc aatcgggaag gtttcaaaca gttttaagct
     5221 ttcttcgttt tgagcgatat attcaagggt ctccagcatt gttcggctgc tgacttgatt
     5281 atgacgggaa agccctgagc catcagccac cacgctgttt ttaaaatcaa tattggcttt
     5341 ggtttttaat aactggcgaa tcacataact acctaattgg aacgatgcgg ggcggttgtg
     5401 ttgcttgttg gcaacggttc taaacagggc atcggcaatt tggttatccg attttttcat
     5461 catttttttc aataataccg gcaacggctc ggaataatgc tcggcaagta gcgtgccgtt
     5521 ttgggcagta agtggctctt ttacttgccc gttaaaggca attttcaggc tttttaactg
     5581 ggctttaagc atatttgccc cgtaattagt tgggtcttgc accgaaaaac ttaagccgaa
     5641 cggttgagac tggcgagcca tacagccttt gatttggtag cggttgttat cgtgaaccac
     5701 cacatcaagc tggcaaaacg gagcttcttt cggctcgaca atataagcag agctaaacac
     5761 ctgcaccgga taagcggacg gcacattcac tttagcaaat tcgccaatgg gttggtcggc
     5821 atttaaggtg acataaaaac agttgtgatc cacattaatt gccgccggtg gggcgttaaa
     5881 gcacatcgtc aagtcgttcc aaatccaacc ggaggctttg tcgtggctgg cgaaaaccga
     5941 cgggtctaga attaaatccc cctcaatttt attgatacct tgttgcttta atttgctcat
     6001 taattgataa atttgaccgc ttgtaagctc agggtcgcca ctaaaacggg caattaaatt
     6061 gccttttaac actccgtttt ctacttttcc gttggttaaa agggcagttt gaaagcggaa
     6121 atcatcgttt aaagttaatt ttgctgccag tgccgtaaag actttttggg tactggcagg
     6181 tagcataaaa atatcacttt ggtattgggt gatgatttgg ttagtgtcta aatttttagc
     6241 aataaaacta acagaagtac cggcaggcaa ggtatttaat aattcttcgg tattaatttc
     6301 agcctgt
//
LOCUS       DANLUA010000145         6244 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLUA010000145 DANLUA010000000
VERSION     DANLUA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..316)
                     /locus_tag="PXB44_002022"
     CDS             complement(<1..316)
                     /locus_tag="PXB44_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282469.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDKVLVKNPSPISIEKNLINAYKEYRKFEEDLKDKIHSQE
                     SIY"
     gene            complement(350..1636)
                     /gene="purD"
                     /locus_tag="PXB44_002023"
     CDS             complement(350..1636)
                     /gene="purD"
                     /locus_tag="PXB44_002023"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL5282470.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1870..2244
                     /gene="rpsF"
                     /locus_tag="PXB44_002024"
     CDS             1870..2244
                     /gene="rpsF"
                     /locus_tag="PXB44_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL5282471.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2219..2593
                     /gene="priB"
                     /locus_tag="PXB44_002025"
     CDS             2219..2593
                     /gene="priB"
                     /locus_tag="PXB44_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL5282472.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2568..2795
                     /gene="rpsR"
                     /locus_tag="PXB44_002026"
     CDS             2568..2795
                     /gene="rpsR"
                     /locus_tag="PXB44_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL5282473.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2811..3260
                     /gene="rplI"
                     /locus_tag="PXB44_002027"
     CDS             2811..3260
                     /gene="rplI"
                     /locus_tag="PXB44_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL5282474.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3324..4100)
                     /gene="dnaQ"
                     /locus_tag="PXB44_002028"
     CDS             complement(3324..4100)
                     /gene="dnaQ"
                     /locus_tag="PXB44_002028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL5282475.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4188..5858)
                     /gene="ettA"
                     /locus_tag="PXB44_002029"
     CDS             complement(4188..5858)
                     /gene="ettA"
                     /locus_tag="PXB44_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL5282476.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1648 a         1423 c         1305 g         1868 t
ORIGIN      
        1 catatatact ttcttgagag tggattttgt cctttaaatc ctcttcaaat tttctgtatt
       61 ctttataagc gttaattaaa tttttctcaa tagaaattgg agatggattc ttcacaagaa
      121 ctttatctcc cttaccttga ttttcttttc tatttaaaga tctttttagt ctttgccaat
      181 attgacagtt ttttccacat atagggcacg cagagtcatt tagaaaagag tttactgcat
      241 cgtttaatga tagtgattta atttcggggc ctgcatcgag ttcagtgata ttaatttcat
      301 taagtgttgt actcataata aatttttcct tgttttaaga gtttataaat tatttcgctt
      361 gttttcttgc cactgctcgg tagccaatat ctcggcgata gaatttgcct tgccattgaa
      421 tttgttcggc aagttgtaag gcttgttgtt gagcttcaaa tacactgttg ccaagtgcgg
      481 tggcacaaag tacacgtcca ccgttggtga gcagttttcc ttctttttgc tcaacccctg
      541 ctaagaaaac tttttgattg gaggtagagg tcggaatgcc tatgatttca tcgcctttac
      601 ggtaatcgcc cgggtagcct gcggcagcta aaacaatgcc taatgcggct tgttcacacc
      661 attttgattg cacgctatcc aatttgcctt cgcacgcttt taagcacaat tcaacgaggt
      721 ctgattccaa acgcatcata atcggctgag tttcagggtc tccaaaacgg cagttaaatt
      781 caatcacttt cggctgaccg tttggcataa tcattaagcc ggcatagaga aaaccggtgt
      841 aaggggtatt ttcgcttgcc attccttgca cggtcgggta aataatttcg tccattacac
      901 gttggtggat tgcctttgtt accaccggag caggggagta agcccccatt ccgccggtat
      961 ttaagccgct atcgccctcg ccaacacgtt tgtggtcttg tgaggttgcc attggttcga
     1021 catttttgcc gtccaccatc acaataaaac tggcctcttc tccgtctaaa aattcctcga
     1081 tcaccacacg gctgccggct tcaccaaagg cgttgcctga cagcatatca cgcactgcat
     1141 cttccgcctc ttgtaaagtc attgcaacaa ttacgccttt gcctgcggct aaaccgtctg
     1201 ctttgattac aatcggtgca cctttttgtt ttaagtaggc aagtgccggt tcaatttcgg
     1261 tgaaattttg gtattctgcg gttggaatct gatggcgagc caagaaatct ttggtaaatg
     1321 ctttagaacc ttctaattga gcagcggctt gggtcgggcc gaaaatggtt aaaccgtggg
     1381 ctcgaaaagc atcaaccacg ccaactacta acggtgcttc agggccgaca atcgtcaaac
     1441 ctacgctatt ttgtttagcg aatgtaacta aggcaggcac gtcagtggcg gaaatagcta
     1501 cattttcaat gccgttttcc atcgctgtgc cggcattgcc gggggcgaca aagactttgt
     1561 ctgcgagcgg cgattgacga actttccaag caagagcgtg ttcacgccca ccgttgccga
     1621 taattaaaat attcattggg attccttgca aaaatgataa agcaaacgtt tgcgtattgt
     1681 acttgatttg tttatctgct gacaagcagt cgtttttctc ggattttttg caaaatacga
     1741 aattgttgct tgcaaaatag gcttttttca gtataatcct gccgttcagt cacattcgtt
     1801 ttttaatcct tgcttccaca agttggtgac tggctaaagt tggaggctgc taacccgtaa
     1861 ggatagtcaa tgcgtcacta cgaaatcgtt tttatggttc acccggacca aagcgaacaa
     1921 gtaccagcaa tgattgagcg ctacacagcg tctgtaaaag aagctggcgg tcaagttcat
     1981 cgcttagaag attggggtcg tcgtcaatta gcatacccaa tcaacaaact tcacaaagca
     2041 cactatgtgt taatgaatgt agaagcacct caaagtgtaa tcgacgagtt agaaactaac
     2101 ttccgttata acgatgcagt tcttcgtaac ttaatcgttc acactaaagc ggcagtaact
     2161 gaagcgtcac caatggtgaa agcgaaagaa agcaaagttg ctgaagcggt agctgaagtt
     2221 gaatcagagg aagcaggcga gtaatttgcc gattgataat tgtttaatct taagcggaac
     2281 ggtttccagc ttggttaaac aaagcctaaa cccccttggt gtgcctcatt acagtttttg
     2341 gttagaacat cgttcaatcc aaaccgaagt gaatttagaa cgccaagcgt ggtgcaaaat
     2401 ccaagtggtt ttggcaggca atcaatttag ttatttaacc caacaaatta agctcggtga
     2461 gagcgtgaga attaaaggtt ttatccatac tcacaaagac tttaacggtt taaatcagtt
     2521 agtcttacac accgaacaaa ttgaatttat agatcaggag aagccaaatg gcacgttatt
     2581 tccgtcgtcg taagttctgc cgtttcacag cggaaaatgt tgttgaaatc gattacaaag
     2641 atatcgctac attaaagaac tacatttctg aaagcggcaa gattgttcct agccgtatta
     2701 ctggtactcg tgcgaagtat caacgtcaat tagctcgtgc aattaaacgc gctcgctacc
     2761 ttgcgttact tccatatact gataaccacc aataatcagg agagagtaca atgcaagtta
     2821 ttttattaga caaagttgct cacttaggtt ctgtgggcga ccaagtaacc gttaaatctg
     2881 gttttgctcg taacttctta atcccacaag gtaaagcggt tatggcaact gcggcaaaca
     2941 ttgctcactt tgaagcacgt cgtgcagaat tagaagcaaa agctgcagca gcattagcaa
     3001 ctgcacaaga acgtgcagcg aaaattgcag cattagcagc agtatcagta tctgcaactg
     3061 caggtgatga tggtcgttta ttcggttctg tatcagctaa agacatcgct gaagcattaa
     3121 ctgcagcagg tgttggagtt gctaaatctg aagttcgttt aggtgaaggt ccacttcgta
     3181 caaccggcga acaccaagtt aaaattcact tacaccctga agtgaatgca actgtagcag
     3241 taaacgttgt tgctgaataa tcagtagaaa tagaaaaccc agcgaaagct gggttttttg
     3301 tttgctttat ttattggtta gaatcagtga gatatttctg ctgtgtcggt taatgctttt
     3361 gtccataggc aatttccttt ggattttttc tcaatcagtt tcagaagtgc taaatgcgac
     3421 tcttcctctt catcgtttgc tttaagaata attgcctcat cggcatttaa cacaattgca
     3481 gaaaattctt cttcaatatc aataacttca cttaccactt cagtttcttc ttcgcctaat
     3541 aatgaaatct gaccgccggt catcattagg aatacatcag ctaagatctc cgcatccagt
     3601 aacgccccgt gtagtacacg cttgctatta tcaatgccga gcctgtcgca aagtgcatct
     3661 aaattattcc gtttgccggg gtacattctt cgtgccattt gcaggctatc ggttacggta
     3721 cacatttctg cggtttttgc cggtggatta ggcagataag aaaattcttg atccataaac
     3781 gccacatcga agggggcatt gtgaataatt aattccgcac ctttgatgaa atccaaaaac
     3841 tcctcggcaa tttcagcaaa aacaggttta tcttgcaaaa attcattggt gatgccgtga
     3901 actgcaatcg cctcttcatc tacctcacgc ggtggcttga tataaacgtg ataagttctg
     3961 ccggttaaac ggcggttaat gacttcaacc gcaccgatct caataatatt atgcccaatc
     4021 tgtggcgggc cacctgccat attcattcct gtggtttcag tatccagcac tacttggcgg
     4081 ataatttttt cttctgtcat attcaattta aaattgtagg ggtgaaatat ttttatccct
     4141 taattctgca tttgattagg ggcgaaacat atttcgcccc tacattgtta cttcgcaatc
     4201 cgtttatatt tcactcggtg cggttgggtg gcggcttcac cgaaggtttt cttcttccac
     4261 tcttcatagt cggagaagtt accctcgtag aacgtcactt tgccttcatc gccgtagtct
     4321 aaaatatggg tggcgatacg gtctaagaac cagcggtcgt gcgaaattac cattgcacag
     4381 cccgggaact ctaagatcgc attttccaac gcacgcaggg tttctacgtc taagtcgttg
     4441 gtcggctcgt ccagtaacaa cacgttaccg ccacgttgta gtagcttcgc caagtgtaaa
     4501 cgaccacgtt caccgcctga aagctcgccc acacgttttt gctgatctac gcctttgaag
     4561 ttgaaacgcc ccacataagc acggcttggg atttcaaagt taccaatcgt gagaatatcc
     4621 tgcccgttcg acacctcttc ccacacggtt ttcttatcgt ccatcgcatc acggaactga
     4681 tcgaccgatg ccattaccac cgtttcacca agcacgattg aaccagaatc cggctgttct
     4741 ttaccagaaa gcatacggaa caaggttgat ttacccgcac catttgcccc gataatcccg
     4801 acaatcgccc ccttcggaat agcgaacgac aagtcgtcaa tcaaagtgcg atcgccgtaa
     4861 gatttagtca ggttttgtac ctcgatcact ttatcaccta agcgtggacc cggtggaata
     4921 aagagttcgt tagtctcgtt acgtttttga tattcgcctg agttaagctc ttcaaagcgt
     4981 gccatacgag ccttactttt cgcttggcga ccttttggat tttggcgtac ccattccaac
     5041 tctttctcaa tcgatttttg acgagccgat tccgccgctt gctcttgtgc caagcgtttt
     5101 tctttctgct ccaaccaaga agagtagtta ccttcccaag gaatgccttc accacggtca
     5161 agctctaaga tccagcccgc aacgttgtct aagaagtaac ggtcgtgggt aattgccact
     5221 acggtgcctt cgtagtcgtg gaggaaacgc tccaaccacg ccaccgattc cgcatctaag
     5281 tggttggtcg gctcatctaa caataacata tccggttttt ccagcaacag acggcaaagt
     5341 gccacacggc ggcgttcacc ccctgataaa tgctcgattt ttgcgtccca atccggcaga
     5401 cgtaacgcat cggcagcacg ctctaactgg ttttgaaggt tatggccatc gtgggcttga
     5461 ataatcgcct ctaattccgc ctgttttgcc gctaatttgt cgaaatcggc attctcatca
     5521 gcatacatcg catagacttc atcaagctgg gttaaggcgt ttttcacttc cgcaaccgct
     5581 tcttccaccg cttcacgcac ggtttgctgt ggggcaagtt tcggttcttg tggaagatag
     5641 ccgattttaa ttcccggttg tggacgagcc tcgccctcga actctttatc tacgcccgcc
     5701 ataatgcgta aaagcgttga tttacccgcc ccgtttaagc cgagcacccc gattttcgcc
     5761 cccgggaaaa agctcaggga aatatcttta agaatgtgac gcttcggcgg cacgactttg
     5821 ccgacacggt gcatcgtata tacaaattga gttgacattg gttgtattcc tcacaaaaaa
     5881 attcgcctta ttttacttga atttacagcc aaaatatagg gtggttttgg cttttggtaa
     5941 agtaaccttt tccaatagag atgttatttg aggctgcata gtactatgca tttctcttac
     6001 cttgacaggt aagagaaaca aatcctgaag gaaaaaaatt ttaaggaagt tattctatgc
     6061 aatatgtatg tccaaaaaca ctcaaattat tcaacagcat tgttggttgt tataagggag
     6121 aagattaatt ctctacctca tcaaggcttg attttagatt taatatccat ttctaaagat
     6181 gatttagatg atattttgga gggtaatact gatatgaata ttcatcaatt attaagcctg
     6241 attt
//
LOCUS       DANLUA010000146         6128 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLUA010000146 DANLUA010000000
VERSION     DANLUA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..1940
                     /gene="iolC"
                     /locus_tag="PXB44_002030"
     CDS             24..1940
                     /gene="iolC"
                     /locus_tag="PXB44_002030"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL5282477.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2229..3569
                     /locus_tag="PXB44_002031"
     CDS             2229..3569
                     /locus_tag="PXB44_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL5282478.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3572..4912
                     /locus_tag="PXB44_002032"
     CDS             3572..4912
                     /locus_tag="PXB44_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5282479.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5050..5874
                     /locus_tag="PXB44_002033"
     CDS             5050..5874
                     /locus_tag="PXB44_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5282480.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5918..>6128)
                     /locus_tag="PXB44_002034"
     CDS             complement(5918..>6128)
                     /locus_tag="PXB44_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5282481.1"
                     /translation="NALGNKENIKQVEACLTRLRVVLHNNGLLNKSALKKLGAVDVIK
                     VGDTQQIIFGAKAGQYRDEINALIG"
BASE COUNT         1728 a         1311 c         1381 g         1708 t
ORIGIN      
        1 tttacttgtt gtaaggagcg gttatggcaa cagttaatca tcaagatcgt ccattggatc
       61 ttatctgttt agggcgtgtg gcggtggact tttatgggca acaaatcggt tcccgtttgg
      121 aagagatgac cacattttca aaatacctcg gcggctcgtc aggcaatgtg gcttacggta
      181 cggcggtgca aggtttacgt tcttcaatgc tggcaagggt cggtgacgaa catatggggc
      241 gttttctacg tgaagagcta caaagcgtag gcgtggacac cagccattta attaccgata
      301 aagaacgctt aactgcactg gttattctcg gcattaaaga taaagagact tttccgctga
      361 ttttctatcg tgataactgt gcggatatgg caatcaccaa agacgatttt agcgaagcct
      421 atattgcttc cagccgttgc ttagcaatta ccggcacgca cctgtcaaac cctaaaaccc
      481 gtgaagctgt gctgaccgca ctcgaatatg cgggtagaaa tgaggtaaaa cgagcgttag
      541 atattgatta tcgccctgtg ttatggggat taacctcact cggtgatggc gaaacccgtt
      601 atatagacag tgccgaagtc accaaatcgc tacaagaagt attgcaccat ttcgatctga
      661 ttgtcggcac agaggaagaa tttcatatcg caggcggttc gaccgatact ctcactgcac
      721 tcaaaaatgt gcgtaaagtc tctaatgccg tattagtgtg caaacgtggg gcattagggt
      781 gttcagtatt tgaaggcgag ctgccgaatg atttagacgg tggcttgaat gtgtacggcg
      841 tgcgtgtaga agtgctgaat gtgctgggtg caggcgatgc ttttatgtcg ggcttactgc
      901 gtggctactt aaataacgaa ggttgggaac aagcgtgcca ctatgccaat gcgtgcggag
      961 cgttagtggt atctcgtcac ggctgttcac cggcaatgcc gaccaaacgt gaacttgacg
     1021 attatttaac ccgtgcagag agtgtaccac gtccggattt agatgagcgt ttaaaccact
     1081 tacaccgtgt caccacccgc aaaaaacagt gggaaaatct ctgtattttt gcttttgatc
     1141 accgcaaaca gttggtggat atggcaaata aagtcggtgc agatcttgct cgcataccga
     1201 aactgaaaca gctgctatta aaagcgatgg aacaaaccgc aactcaatcc ggcttaccac
     1261 aaaatcaagc agggatccta gccgatacca ctttcgggca agatgtactt aatgagatta
     1321 ccggcaaaga ttactggatt gcccgcccga ttgaagagcc gtcttcccgc ccgctggaat
     1381 tagagtatgg agatttaggc tctcagctag catcattccc acgcgatcac gtcatcaaat
     1441 gcttggcatt ttaccacccg aatgatgaaa gcggtttaaa agcccgccaa gatcgcaagt
     1501 taaaagaggt gtatcaagcc tgttgccgta ccggtcacga gctattgttg gaaattattc
     1561 tgcctgctga tatgccacag agtgaggctt tctataacca agcgatagca catttctacc
     1621 aacttggcat taagccggaa tggtggaaat tgccggatgt ttctcgcgca caatggcaag
     1681 cgatttccgc caccattgag gcaaacgatc cggactgtcg tggtatttta attttaggtt
     1741 tagatgcccc gttagaggtg tttaaagaca cctttgaaca ggcaaaaggc tgtgagaaag
     1801 tgaaaggctt tgcagtcggc cgcaccattt tcggtgaacc gtccgaaaaa tggcttgccg
     1861 gtgaattgga cgacaatacc ttgatttctg cggtatcgga gaaatatcgc actttaatcg
     1921 atctctggca acaacgttaa tcatattgct ttaagtgaaa accccttagc ttatgatatt
     1981 aagcaagggg ttttgttttg caaaaaatcc ggctaaacag accgcttgct agccgtaaaa
     2041 atgcccgaat tttcctaaaa aagcgaatat cgggcattcc tcaatctgct tatcacaaaa
     2101 taagtgtgat gaagttcaca tttcatacat aaaccccccc ctttttatcg aaacaaattg
     2161 ttttattttt atcaaaatgg aaaatgtatt tcagtttagt tctatttaga ttcaatagga
     2221 gtctgtgtat gagttgcaac acttcaccaa aacgtttcca cgccagttgg gagtcgatca
     2281 aacagcacca agtaccagag tggtatgaag atgctaagtt tgggattttt attcactggg
     2341 ggatttactc cgtacccgcc tttgccccac cgacctgtca gttaggtgaa attgatattg
     2401 atgaacagtg gttctgtaat aacccttatg cagaatggta ttttaattcc atcaatgtca
     2461 aaaaaggccc gacctacgaa catcatatca aaacttatgg cgaatccttt gcctataacg
     2521 acttcctgcc actatggaaa gcggaaaaat ggcaaccagc agaatgggcg aaactgtttg
     2581 ccaaagctgg cgccaaatat gtggtattaa ccaccaaaca tcacgatggt ttctgtctct
     2641 atccaagtaa atataccgat ttcaactgta tgcaagccgg accgaagcgg gatttaatgg
     2701 gcgagctgac tgaaaatgtg cgggagcaag gcttaaaaat gggcgcttac tactcgggca
     2761 ttatcgattg gacttattcc tctgccccga ttttcaccga aagtcaaaac ttctcaaacg
     2821 cttgcccgac ttacgaatac gctgactacg cctataaaca agtggtagag ctgatcgacc
     2881 gctataaacc agatgtattg tggaatgata ttggctggcc aaaagtgggc gagcatatgt
     2941 taccacacct atttgctcat tattacaaca gcgttcaaga gggcgtagtg gacgaccgct
     3001 ggaacaaact atggtgcgat ttcacctcca aagaatacaa acacggcgtg gcttcccgtg
     3061 ataagaaatg ggaaatgtgc cgtggtatgg gcttatcgtt cggctataac caggtagaag
     3121 acgaaagcca tttgatttcg gtgaaagact tgatttccct attggtagaa accgtagccg
     3181 acaacggcaa tttattgctg aatatcggcc cgaaagcaga tggcactatt ccgcaagagc
     3241 aagttgaacg cttattagca ctggggagct ggctggaaat aaatggagag ggaatctata
     3301 aatcccgttg tagccaacac caaaccgagc aaatcggtga tgtaacctat cgttatacca
     3361 aagttggggc ggatctctat ctttacattg atggcttaac cgaagcccaa atcagcttgc
     3421 cactgtcagt ttatggtgag ctacaagcac tcaataccga gctgaaattt accattgaaa
     3481 acaccgcaca aggcagagtg ttacatattc aaaactacca gccggattgg tatgtattag
     3541 gctttaaaat tgcagatggg gagaaatagg tatgtccgtt tcacaacgtt ttattgactt
     3601 tatgaatgcc cacgttgctc cagtagcacg tcgaatggaa aatcaaccgc atatttccgc
     3661 tattcgagac ggttttattg tagtgttacc attcttaatt gtcggcagct ttattatgat
     3721 tttgctaatt ccgccatttg atgaaaatac ccaaaatgct ttcgggcaag cttggtggcg
     3781 ttttgcgaat tgggcaagcc cttacggttg gaatttcttc caaatgtcgt ttaatgcgat
     3841 ttcattattt acctctgcca gtatcgccta caaccttgcc aaagcctata aaagagagcc
     3901 cctgccagcg gcatttctct cggtaatggc atttttgtta gtggctgcac cggtcaaaga
     3961 cggaatgatg gatattaaat tctttggcgg tattgggcta ttctctgcga tttttattgc
     4021 catttattcg gtagaaatga cccgcttgtt ggagttctta aaaattaaaa tccgcttacc
     4081 gaaagaagtg ccgcacgctg tagcagaatc tctgaatatc gtgattccaa ttttagcgat
     4141 tttactgact ctctatccgt ttgccatttg ggtggaaagt gccacaggtc gtaatattcc
     4201 acaactaatt atggacttta tggcgccatt gattgctgtg agtgactcgc taggggcaat
     4261 ttgcttgttc gttattgcta cccatttgct ctggttctta ggcattaacg gctcattggt
     4321 actaatgcaa ctttggacac cattcctact gcaaaatatg gcggcaaact tagcagcatt
     4381 ccaagctggt gaaccgctcc cctttatcat caccaattcc ttctgggatt tctatatcgt
     4441 acacggggca agtggtggtg taatagcctt ggcattcctg ctggtacgca gtaaatcggc
     4501 acacttacgc tctatcggta aaatcggctt ggttccgtcc ttcttctcta tcggtgaacc
     4561 aattgtgtat ggtgtgccaa tggtggttaa tccgctgttc tttatcccgc tgattttcgc
     4621 accactagcg aatgcggtta ttgcctacct gattttagat tttaacctta ttcaccgtgt
     4681 ttacttgatg gcaccgtgga caacaccggc accgattggg gcatatctgg tttctgccgg
     4741 tgatatttgg gcaccaattt taagtattgc actgattatt ttggatattg tgatctacta
     4801 tccattcttc aaaatgtatg aaaaagtctg cgtcgagaaa gaaaaagcac aaaatctaga
     4861 tgaaaaagca caacaggagt tgctagaaaa agcacgaatg gcagcacaat aacacttatt
     4921 tctagcgtaa ttgagagggg ctaatcgaaa gattagccct tatttttgca aaaaattgac
     4981 aaaatttatc cgcttgtaaa caagaaaagc gtataatgaa ataaaaaatt caagcagaga
     5041 taatttgaaa tgaatggacc tactcaatta gcacattttc aagaacaaat ccgaaaagag
     5101 tatgacggac taagtaaacg tttaaaacaa gtggcacagt atgtgctgga taatggcaac
     5161 agcgtggtgt ttgatacggt ttctaccatt gctgaacgag ctgatgttcc cccttcaacc
     5221 ttaatccgtt ttgcgaatgc gtttggattt agcggtttta atgaaattaa acagctttat
     5281 cgccaagata tgatggaagg cacatcacgt tacaccgaac gtgtgcaact ttttcgtcaa
     5341 attgaaccgc ttgatgagca aaacagtgag ctcaatcata ttttagatgt gttcgtacag
     5401 ggtaatacgc aggcactgca acagttatcg acacaagtca gcgaagagca gttagcacaa
     5461 acggtagata ttttacacaa tgccaaacgc atttttattg tgggtttaaa acgctcattc
     5521 agcattgcga actacctaaa ctatgcgttg caccatttgg actatgatgt ctttatgatc
     5581 gatggttcgg gcggtatgtt tgacgagcaa ttaagccgaa tccgtgaagg tgatgcggtg
     5641 attgccgtga gcttttcccc gtatgcgaat gaaacactca acgtggtgac agcaactgcc
     5701 agaccggggg taaaacatat tgcgatcact gatagccaac ttagcccact cctttcattt
     5761 agcgatgtgt cgtttattat taaagaagca caggtgagtg gttttcgatc ccagtgtgcc
     5821 acaatgacgc tcgcacaaac gattgccgtt tcactggcgt tgaaaaaagc ctagattaag
     5881 aaagtgaaag acctgcgtaa atacaggtct ttgtctatta accgattaaa gcgttaattt
     5941 catcacgata ctgcccggct tttgcaccaa aaataatttg ttgcgtatca ccgactttaa
     6001 tgacatcaac cgccccaagc tttttcagtg ccgacttatt taataaaccg ttgttatgta
     6061 acaccacacg taaacgggtt aaacaagctt caacttgctt gatattctct ttgtttccta
     6121 aagcatta
//
LOCUS       DANLUA010000147         5940 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLUA010000147 DANLUA010000000
VERSION     DANLUA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            4..852
                     /locus_tag="PXB44_002035"
     CDS             4..852
                     /locus_tag="PXB44_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5282482.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
     gene            940..1758
                     /locus_tag="PXB44_002036"
     CDS             940..1758
                     /locus_tag="PXB44_002036"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL5282483.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            1761..3452
                     /gene="narQ"
                     /locus_tag="PXB44_002037"
     CDS             1761..3452
                     /gene="narQ"
                     /locus_tag="PXB44_002037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL5282484.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQ
                     QLLKDWYEMQSYVAQGDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKVQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPGER
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETNDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            3503..4021
                     /locus_tag="PXB44_002038"
     CDS             3503..4021
                     /locus_tag="PXB44_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5282485.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            4064..4663
                     /gene="rdgB"
                     /locus_tag="PXB44_002039"
     CDS             4064..4663
                     /gene="rdgB"
                     /locus_tag="PXB44_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL5282486.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            4660..4980
                     /locus_tag="PXB44_002040"
     CDS             4660..4980
                     /locus_tag="PXB44_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL5282487.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            4984..5436
                     /locus_tag="PXB44_002041"
     CDS             4984..5436
                     /locus_tag="PXB44_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL5282488.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(5477..>5940)
                     /gene="lpcA"
                     /locus_tag="PXB44_002042"
     CDS             complement(5477..>5940)
                     /gene="lpcA"
                     /locus_tag="PXB44_002042"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL5282489.1"
                     /translation="FKQGGKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISD
                     VSHLSCVSNDFGYEYVFSRYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVI
                     AMTGKDGGQMAGLADVEIRVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
BASE COUNT         1934 a          965 c         1287 g         1754 t
ORIGIN      
        1 aaaatggaaa aaaaagccct tttccgtgga tttttattat cgacagtggc attagcagtg
       61 gctgcttgtg gtaacttaag taaagtgagt gatgaaggaa caactgaaaa cccggtattc
      121 ccgaaaatct cagaatctga atttaaccac gatggttcac aatttggttc atggccaaac
      181 tgggaaaacg ttcgtcaaat tgaaaaaggg atgaacaaag atcaattata taatctaatt
      241 ggtcgtcctc attttgaaga aggcttatat ggtgttcgtg aatgggacta tgcattcaat
      301 taccgtgaaa atggcgtaca taaaatttgc caatttaaaa ttttattcga taaaaatatg
      361 aatgcacaaa gtttcttctg gtatccaaat ggatgtaatg gaaactcatc atttgcttta
      421 actggcgatt tcttattcga tttcgataaa gcgactttaa ctacaaaagg taaagaagtt
      481 gttgataatg ttgctcaaca attaaaagca tctaaagcac aacaagttaa agtagcaggc
      541 tatacagacc gtttaggctc tgctgcttat aacttagact tatcacagcg tcgttctaat
      601 acagttaaag ctcgtttagt acaacaaggc gttacagcac aaattgaagc ggtaggttat
      661 ggtaaagcga atcaagtaaa agcttgtgat ggtgaaactg gtcaagcatt aaaagattgt
      721 ttacgcccta accgtcgtgt tgaaatttct gcaaattgtg gtgtaatgaa acaaaatgaa
      781 ggtggcaatg ttgcaggtcc taatggtccg gctccacttt atcaaacccc agcatatgat
      841 ggcagtaaat aatttctagt tttgactaac ttagagtaga atattaaggc aaccaaatgg
      901 ttgccttttc ttttatctat ttatttatag gggaatacta tgaactacca agctattgca
      961 tttgatttag acggaacact actttcatcg aacgcaacaa ttttagaatc cagtaaagag
     1021 gctattaaaa aggtaagaga aaagggaatt aaagtctact ttgttaccgg taggcaccat
     1081 actgcggttc gcccttatta tgtagaacta gggctggata cgccagttgt gtgttgcaat
     1141 ggcacttatg tgtatgattt taaaaatgaa aaagtattaa gcggtaatcc gttatcagca
     1201 gaaattgctt caaaattagt taatgatgca caagctcaag ggattcatac tgcggtttat
     1261 tttgaagatg caatgactta tgagtctttg aatattcact tcactaaatt ccaaaaatgg
     1321 gttgagtctt gcgcaccaga agttcgtccg aatgtttatc aggtagaaaa tttccaacaa
     1381 gaaattgata aaggtgtaac cgtatggaaa gtgcttatta gcgatccaga tcttgccaaa
     1441 atgcaaaaat ttgttgaaaa attaccgcct gatcaagtaa gcccggaatg gtcttgggtt
     1501 gatagggttg atattaccag tgttggtaac agcaaagggg caagattagc ggaagtatta
     1561 aaaacagaag gtattgattt acaaaaggtg attgcctttg gcgataattt caatgatgtt
     1621 tctatgctag aaaccgttgg attaggcatt gcaatgggaa attcagaacc agatattcag
     1681 caaaaagcga agaaaactat aggttcaaat aatgaagata gtattgctaa tgaattgaaa
     1741 gctcaattta atctttaatt atgataaaag caaaatactc tattgctagg cggataggac
     1801 tctatttttt aattatgatc ggatttgctt cgattatcag tggaatctca cttggtatta
     1861 tgtggagcaa taaatctgat gcgagcctga ttaatgtttc aggctcactt cgtatgcaaa
     1921 gttatcgatt gttatcagaa atggattcaa aaaaagcgct gctacctgag cgattgttgg
     1981 aatatcatcg aacgctacat gctacagagc ttagttcatt aaagcaaagt gttttattgc
     2041 cagatagcgt gatagaaagc tatcaacaat tacttaaaga ttggtatgaa atgcaatctt
     2101 atgtcgctca gggagataaa gttaattatg cttcgcacat tgaagattat gtgcagagag
     2161 ttgatgattt tgttttccgc ttacaagagt tcgctgagtt aaaattaaag attgcaacag
     2221 gcgtgattgc gattgcaatg ttactgatta ttgcccttgc ttatattggc gtttggtata
     2281 ctcgaaaaaa gattattgga cctttaaggc aattggtaaa agcgagttgg caaattcaaa
     2341 ataaagattt tgaccacgta aaattagcgg ttaatgaacc caatgaatta gggctacttt
     2401 cctctacttt tactgagatg tctaatgaat tattaaaatt atatacttca ttagaagata
     2461 aagttcagga taaaacacgt cgtttattgg cggtaaatcg ttctttactg gtattatatc
     2521 aatgctctca attattaact gctaagccga ttaataaaga agtgttaaat caagtactta
     2581 aaacggtatt tgataatgaa catttgctag ggattgattt acaagtatat ggggctgaat
     2641 attggaatgt atcaatagac aataaaccaa gtttagattg ggtttctgct gaaattgcaa
     2701 tagaaaatga aaaattgggt atcttaagct ggaaaccctc actggtttgt cctggtgaac
     2761 gattgatcca aaatgtgtcg gaaatgattg gtcgtagcct ttatgtagtg cagatgcaaa
     2821 agcaacagca acaattggta ctcatggagg agcgtgcaat tattgctcgt gagttacacg
     2881 attccttagc ccaatctctt acgtttttca aaattcaggt tagcctgctt aagcttgcta
     2941 aaacagatga aaagcgtaat gaaattttaa ctgtatttga aaaagcgtta aatgatgctt
     3001 acagccaatt aagggagctg ttgacgacgt ttaggctaac gattcaagaa gcgaatttac
     3061 aacaagcgtt agaaaaaatt ttagaaagct taagaccgaa aacctcagca cagattcgct
     3121 taagttgcaa attaccttcg catatgttta atgctcagca gcaagttcac gtattgcaaa
     3181 ttgtacgaga agctgtgatt aatgctatta agcattcgca agccactgaa attaatgtga
     3241 ttgctgaaac aaatgatgat ggtgaacatt gtttgactat tcaagataat ggcgtagggc
     3301 ttgctgccca tacgcaacca gaagggcatt atggactgac gattatgcaa gaacgggcgg
     3361 ctgaactcaa agccgaattt tcgattgaaa atgtggaaag tggcggagta aaagtacagg
     3421 ttttattgcc gagtatgatt agcaagcggt aataaaatat taattttttg cgtattattg
     3481 gcaacgaata aaaggataac aaatgcaatt ccgaaaagca gtagaagcgg attttgagac
     3541 aatattggca atttataatc aagcaattcc cactcaccaa attaccgccg atttagaact
     3601 ggcaactacg caaaatcggc ggaagtggtt cgattttcac cttgaaacag cccaatatcc
     3661 tatttgggtg gtcgaagatg aaaaaggcat tgctggctgg tttagttttt caccatttta
     3721 tgaaagaccg gcatttttac atacttcgga aatcagtatc tatttagacc gctattccaa
     3781 aggaaaaggc tatggctcaa aaattattga gtttatgcaa gctgaaatgc ttaatcacaa
     3841 tattcatacg ttgatggcgt atgtgtttga attaaataca gtgagccaaa atctaatgag
     3901 aaaacacggc tttgaacaat ggggaagatt tccgaatatt gctaatatgg gaaaagatga
     3961 gaaaggtgag gataaatggc gaacgttgct gatgatgtct taccaaaaag gtatcgaata
     4021 acaagcggtg attttttaga atatttttgc aaataaggac aatatggaaa agacaaaaat
     4081 tgtacttgca acaagtaatg cagataaagt taaagaaatg gcagatgtac tctcacaatt
     4141 tggttttgag gtggtggctc aaagtgaatt tgggattgaa tcaccggaag aaaccggttt
     4201 gacttttgtt gaaaatgcgc tgattaaagc acgatttgct gctaaaatga caggtttacc
     4261 ggcgattgcc gatgattcag gtttatcggt tatggcactt ggtggtgaac cggggctata
     4321 ttctgcacgt tatgccggag agcaggcaac cgatgccgat aatcgccaaa aattactagc
     4381 caaaatgcaa gggcaagaga atcgtctggc gaaatttgtc agttgtatcg tgtttttaaa
     4441 acacgaaact gacccaacac cttatattgc gtcgggtgaa tgtttcggtg aaattttgac
     4501 ggaagaaaga ggtgaaaatg gtttcggcta cgattccctg tttttctacc caccgaaagc
     4561 ttgcactttt gccgaattag aaaccaaaga gaaaaaagcg atttcacacc gtgcaatcgc
     4621 actagagcaa ttaaaacagc aattagcagg agagaataaa tgattattac aactacacct
     4681 acgatagagg gtaaacaaat tgtagaatac aaagggttag tatttggcga agtagtttct
     4741 ggggctaatt ttatccgtga tttcttagcc ggtatcaccg atattattgg cggacgttca
     4801 ggtgcttatg aaagcaaatt aaacggtgca agaaaagagg cattagctga attagctaag
     4861 gaggcacaaa aagtgggggc aaatgcggtg gttggcgttt ctatggagta tcaatcaatg
     4921 ggcggcgata aaggaatgtt catcgtggta gcgactggta ctgcggttgt aatacgttaa
     4981 tttatgcagt ttgaacaaca ctttgaagca aatttctggc agaacaaatc gctacttgaa
     5041 atgaatgatg ctgaatggga agcattatgt gacggttgtg gtaagtgctg ctaccggaaa
     5101 tatattcagg gaagagggcg gcgtgaacgg ctttactata cctcggttgc ctgtaattta
     5161 ttagatgtga atacaggccg ttgtatgaat tactcgaacc gttttaaaat tgagtcggat
     5221 tgtacaaaat taactaaaaa gaacttacct gattttggct ggttgccaaa aacctgtgct
     5281 tatcgtttac tttatgaagg aaagcctctt tttgattggc accctttaat ctctaaagat
     5341 cctgattctg taaaaaatgc cggaatgctg attgctaacg gtattcacga aaaagatgtg
     5401 attgattggt ttgagtttgt tattgatgaa gtatgagaaa aaggcgtttg gtgcaaacgc
     5461 ctttttgtta tagagattag gctttttcca tctcaaattc aattaacatc atcaaaatat
     5521 gaatcacttt aatgtgaatt tcttggatgc ggtctgcgta acggaaatga ggtactcgga
     5581 tttccacatc ggctaatcct gccatttgcc cgccatcttt gccggtcatt gcaattactt
     5641 tcatgccttt ttctttggca actttgatcg catttaacac atttttagag ttaccagagg
     5701 ttgatagacc aaagagtaca tcgccttttt gcccaacggc ctctagataa cgagagaaga
     5761 catattcata accgaagtca ttacttacac agcttaaatg gctgacatcg gaaatagcga
     5821 ttgccgggta tccgggacgg ttttcacggt aacgacctgt taattcttcg gcaaagtgca
     5881 tcgcatcaca gtgcgaaccg ccattaccgc aagaaatcac cttaccacct tgcttgaaac
//
LOCUS       DANLUA010000148         5911 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLUA010000148 DANLUA010000000
VERSION     DANLUA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(44..120)
                     /locus_tag="PXB44_002043"
     tRNA            complement(44..120)
                     /locus_tag="PXB44_002043"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Pro,seq:tgg)
     gene            290..1150
                     /gene="folD"
                     /locus_tag="PXB44_002044"
     CDS             290..1150
                     /gene="folD"
                     /locus_tag="PXB44_002044"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL5282490.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            1264..2715
                     /gene="thiI"
                     /locus_tag="PXB44_002045"
     CDS             1264..2715
                     /gene="thiI"
                     /locus_tag="PXB44_002045"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL5282491.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(2760..3467)
                     /locus_tag="PXB44_002046"
     CDS             complement(2760..3467)
                     /locus_tag="PXB44_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL5282492.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(3535..4509)
                     /gene="pfkA"
                     /locus_tag="PXB44_002047"
     CDS             complement(3535..4509)
                     /gene="pfkA"
                     /locus_tag="PXB44_002047"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL5282493.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(4616..5812)
                     /locus_tag="PXB44_002048"
     CDS             complement(4616..5812)
                     /locus_tag="PXB44_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL5282494.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         1771 a         1141 c         1308 g         1691 t
ORIGIN      
        1 ccagctgagc taagggcgca gatgaagata atataaaaag aagtggtcgg cgagatagga
       61 tttgaaccta cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg
      121 acataagatg ttgttgatta tagatttctt tgcacaacag tcaatctttt tttgtgaatg
      181 tcggcttaaa tgattgaagt ttatccgtta ttcaaaatta gcttatcaaa aaggctattt
      241 tttgagagaa tgacgaatgt tttgccaagt gatatttcgg gaggtagtaa tgactgcaca
      301 aattatttcg gggagtgagg tttcttcaca aattaaaaaa aaggtgagtg agaaaatcgc
      361 tcattatctt gaacagggta aacgtaaccc tggtcttgcg gtcattttag tggggaatga
      421 cccagcctct caagtgtatg tggggagtaa acgcaaaagc tgtgccgaaa ttgggattgc
      481 gtcgaaatct tatgatttac ccgaagaaac cacagaggct gaattattag cactgattga
      541 aaagttgaat caagatgatt cggtcgatgg gattttagtc caattaccgt tacctaaaca
      601 gatcgattcg actaaagtga ttgaagccat cttaccacat aaagatgttg atggattcca
      661 cccttataat gttgggcgtt tatctcagcg tattcccact ttacgccctt gcacgccata
      721 cggtgtgatg aagttgcttg aaacaactgg ggtagattta tatggtcaac acgcagtgat
      781 tgtaggtgcg tctaatattg ttggtcgccc gatggcgtta gaattgttgc ttggtggttg
      841 tacagtcaca gttactcatc gctttaccaa agatttagag catcacgttc gccaagcaga
      901 tattttagtt gtggcagtcg gcaagcctga atttattccc ggggagtggg ttaaagacgg
      961 tgctatcgtg attgatgtag ggattaaccg tggagcagac ggcaaactaa aaggtgatgt
     1021 agagtattca gttgcctcac aaaaagcggc atttatcacc cctgtccccg gtggggtagg
     1081 cccgatgacg gtggcaatgt taatgcaaaa taccttgcag acgtatgaaa accatttaca
     1141 agcggtctaa ttttacggat attttgcaaa tcctcggcat tggtcgagga tttctttttc
     1201 tgggcttttt cagcagaaaa ggctaaaata cttgccattt ttattacacg atccttatta
     1261 aatatgaaat ttatcatcaa acttttccct gaaattatga ttaaaagcga ctctgttcgg
     1321 aaacgcttta tcaaaatttt aacctcaaat attcgcaacg tattattaaa agagactgaa
     1381 aatgtgtctg tggttcgcca ttgggacttt attgaagttc gggcgaaagt ggacgaagaa
     1441 acggcattag tgctggattt gcttaaacgc acgccgggta ttcaccatat tttagaagtg
     1501 gaggaaaccc cttttacgga tatgcacgat atttttgagc agacttatgc cgcagtgaaa
     1561 gctgaactag aaggcaaaac gtttagcgtg cgggttcgcc gtaaaggaaa gcacgatttc
     1621 cgttctcttg atgtagagcg ttatgtcggt ggtgggctga atcagcggat tgaaacggcg
     1681 aaagtgaaat taaccaaccc agatgttacg gtcagaattg atattgaaca tgataaaatg
     1741 ttgctgatta aagctcgcca cgaagggtta ggcgggtatc caatcggcac acaagaagat
     1801 gtgttgtcat tgatttcggg tggttttgat tcgggtgtat cgagctatat gctgattcgc
     1861 agaggttctc gtgtgcatta ctgttttttc aatttaggtg gtgccgcaca cgaaattggc
     1921 gtgaaacaga tggcatatca tatttggagc cgttatagta cctcgcacaa agtccgtttt
     1981 atcgccatta attttgaaaa tgtagtgggt gagattttag aaaaagttga taacggacaa
     2041 atgggcgtgg tattaaaacg tatgatggtg cgagcggcaa gccgtattgc cgagcgtttt
     2101 gatattcaag cgattgtgac cggtgaggcg ttggggcagg tctctagcca aacactgact
     2161 aatttacgtt taattgataa ggcatcagat acgctggtat tacgtccgct catcactcac
     2221 gataaagagc agattattgc aatggcaaaa gcgataggca ctgacgatat tgctaaatca
     2281 atgccggagt tctgtggagt aatttctaaa aatccgacgg ttaaggcaat cgaaactaaa
     2341 attttggaag aagaaggcaa ctttaatttt gaggtactag aacaagcggt tcaaacggcg
     2401 agttatttag atattcgtga gatcgctcag caaaccgaaa aagaggttgt ttcggttgaa
     2461 accacaacag aattgagtaa aaacgatgtt gtgctagata ttcgcagccc ggaagaggtt
     2521 gatgaaaaac cattccgttt agacggggtt gaagtaaaag aactcccgtt ttataaactt
     2581 tccagccagt ttagtacgct tgaccaaagc aaaacctact tgctctattg ccaacgtggg
     2641 gtaatgagta aactgcaggc tctttatttg aaagagaatg gctacagcaa tgtaaaagtg
     2701 tttcgagtga aataatgcct ctttgattgc accttaatga ttgattaagg tgcaattttt
     2761 taccaaataa ggcttgctaa aaagagtaag atcacaggaa attcaagcat tccaacttgt
     2821 ttaacattcc agccaaagct tggcacaata atggctcggg cagttgcaat taaataagca
     2881 aaaaagaccg cttgtagaga gcagagtatg taaagcattg ctaaaaccaa atgtattgca
     2941 atactgattt ccatataaat tgggtttctt cgctctcgca ccatactctt tatatagaaa
     3001 gtcgtagcaa taaaaaatag ggtagggtgg agcagaatct caaactgata ttgtttcgtt
     3061 accagataat aagctgccat tcccattatg ccaaaggtta gaatgccggc aatatcattc
     3121 cataaattgc gttcatcttt ttgtttggcg tagtgaactt gcactaaccc aagcggtaga
     3181 attatcggta aaaattgcaa aatcgaaggc tgagcgtaga tcaaaggtaa tgcaaataga
     3241 atgcttaagc caaagtagat tttcgcccat tttttattgc gttcagttgg ctttttactg
     3301 aaaagtgaga gaaaagggta ggaaaagaag tagagaaaca gccacgacag cccaaagaaa
     3361 atatgcaaag tgctaaaacc actttcaata acggcgtaga gaaacggcaa cattgccatt
     3421 gctaatgcac cgtgttggtt ggatataacg ggtttttctt tgaacattca atttccctct
     3481 taatgtggca aataaaaagg cgactaaaca gccgccttta aattgtaaga aaagttagaa
     3541 taattttcta gaggcttcaa ataattcttc tttgaacgga cgacgcatat tattgatcgc
     3601 atcaataata tcgtggtgaa ccaactgctc gttttgaata ccgacacagt gaccgccaaa
     3661 gccttgcagt aataattcca ccgcatagac gcccatacgg gaggccaaaa tacggtcgaa
     3721 ggcacaaggc gaaccaccac gttgaatatg acctaatacg gttgcacggg tttcattgcc
     3781 gaaacgtgct tcaatttctt tcgctaatgc gtgaacatcg gtcatcaatt ctgtaattgc
     3841 aataattgaa tggcgtttac ctttgtggaa accttcttca atgcttcgca ttaatgcatc
     3901 tttatctaag cctttttccg gtacgatcac atattcgcaa ccaccggcga gggcagcact
     3961 tagggttaaa tcaccacagt ggcgacccat aatttccaca atcgaaatac gttggtgtga
     4021 acttgaagta tcacgcaggc ggtcaatcgc atctaatgcg gtttcaaggg cagtttgata
     4081 gccgatggtg taatccgttc ccacaatatc gttatcaatc gtacccggaa taccgataca
     4141 agggtaacca aattcttcgg ttaataattt agcccccata taagaaccgt cgccaccaat
     4201 aaccactaat gcatcaatgc tgtatttttt taaggtttcg acacaatgtt tgcgaacttc
     4261 tggtctttta aaatcaggga agcgggcaga acctaagaat gtaccgcctc ggttgatggt
     4321 ttcagacacg gaacggcggt caagtgggat cactttatcg tggtaaagcc catagtagcc
     4381 gtcttgaata ccacaaacct ctaaaccttc attaagtgcg gtacgaacca caccacggat
     4441 tgccgcgttc attcctggtg catcaccacc actggttaat actgcaattt ttttgatttt
     4501 tttactcatt taaaaacacc ttgttgataa attaaagtta aattggaaac tggtgcaagg
     4561 ttacaatatt cagccttttc gttctactaa aaaatacgta tttttttgat ttagcttagc
     4621 tttcttgcaa aatttcaagg gtaaaatcta gagcgttgaa aaaatgctca acatcttgtt
     4681 gattattgta atgggcaagt gaaagtcgaa ctgttccgac ctcttctaaa tggcgtaaat
     4741 aaggcttggc acaatgctcc ccttggcgga tcgcaatttt actttgggtt aagaaagcgg
     4801 caatatcggc agggtgctga tttttcacca caaagctaat agtcggtgta ttttgttgcc
     4861 cgataatttg caaattttga taagaattta accgcttgca ggtgtattca gccaagtcat
     4921 aaagctgatg attaagagcg acaaaatccc atttttcgag ccaagctagc acttgcccaa
     4981 agccgataat gccggcgata ttcggcgtgc cagcttccaa gcgataaggt aactctgcca
     5041 cagttaattc actttccgta atgtttttca gcattttgcc gccaaataca agcggttgaa
     5101 tttgctccaa actttgcaat ttgccggtaa gcacgcccac accgtttgga ccatacattt
     5161 tatgggcgga aaaagcgtag aaatctgcat ctaatttctg aacgtccact tgctctgagc
     5221 aaactgcttg agcaatatct aacacaattt tagcagagca agattggcgg ataagcggga
     5281 ttatttgttc cgctggctgg cgaactcctg ttacgttaga aactaaatta aaggcaacaa
     5341 ttttggtttt ttccgataac gcatttttaa gggtttcaag ctcaatttca tacttatcat
     5401 taagtggcaa caccacgagt ttcgcccttg tacgctgtgc caactgctgc caaggaataa
     5461 aattagcgtg atgttccgcc actgaaatta caatttcatc gcctgctgca atttgatgct
     5521 ccaagccata agccacggtg ttaatacttt gggttgtacc gcttgtccaa atcaccgctt
     5581 cacgagtttc cacattgaaa cgttttgcga ctaaatctcg agctaattca tattgttcgg
     5641 tttgtggcaa atcgtactga ctgcgatgca ccgaccccgc agaagcgtaa aactcacttg
     5701 ttgcttcaat gagtacatca ggtttcagcg ttgtagcggc agaatctaaa tacgtccatc
     5761 cgtcagattt ttcaaaaaaa gggaaatggg tgcgaaattg ggtgttaagc ataagttaat
     5821 tcctaagagt tgtggaaaat tgagttacca agaaaatacc tattataaag gaatgttttg
     5881 aggtaagaag catttcaaga aataaataga t
//
LOCUS       DANLUA010000149         5850 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLUA010000149 DANLUA010000000
VERSION     DANLUA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            107..538
                     /locus_tag="PXB44_002049"
     CDS             107..538
                     /locus_tag="PXB44_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5282495.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            525..992
                     /locus_tag="PXB44_002050"
     CDS             525..992
                     /locus_tag="PXB44_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5282496.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            995..1762
                     /locus_tag="PXB44_002051"
     CDS             995..1762
                     /locus_tag="PXB44_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5282497.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            1759..2565
                     /locus_tag="PXB44_002052"
     CDS             1759..2565
                     /locus_tag="PXB44_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL5282498.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            2565..3602
                     /locus_tag="PXB44_002053"
     CDS             2565..3602
                     /locus_tag="PXB44_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL5282499.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            3692..4912
                     /gene="agp"
                     /locus_tag="PXB44_002054"
                     /pseudo
     CDS             3692..4912
                     /gene="agp"
                     /locus_tag="PXB44_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628146.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            5220..>5850
                     /locus_tag="PXB44_002055"
     CDS             5220..>5850
                     /locus_tag="PXB44_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282500.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTE"
BASE COUNT         1793 a         1081 c         1269 g         1707 t
ORIGIN      
        1 actttatatt tcttcttatg tgtacgatat atcgtgcaat aacttccctt gtttatctgt
       61 ataacatttt atttaataga atgacttata aatcaaggag tgacttatgt ctaatcaaat
      121 tgcgttattg ttgatgagcc acggcagttt tgcgagggcg gcgttggcga gtgccgagct
      181 gattgtcggg cagcaggaaa atgtggcagc tatcggcgta gtagcagtgg ataatgtagc
      241 ggacattaaa cggcagatgg agcaaaccat tgctgagtta aacaccgaca aaggcttgat
      301 tattctcacc gatattgttg gtggtacgcc gatgaatctt gcctcttctc aattaaccca
      361 ccctaatgtt tttgcctgtt cgggtttgaa tttaccttta ttgctagaag tgctgatgag
      421 ccgtgacggc aatttgcaag aaattcggga aaatctaccg cttgcatacc aaaacggatt
      481 tagcctacgc acacaacaag attttgtttc acaggaggac gatgatgatc tgctttagcc
      541 gtattgatga ccgtttaatt cacggtcaag tagtcaccac ttgggttaat ttacaccgta
      601 ttgaacaaat tattgtgtta aatgatgcgg tggcagcgga tccaacccaa aaacagattt
      661 taacgatggc agcaccgcag ggaattaaag tggtcgcgtt tggcattgaa aaatttggtg
      721 aagtgttgca gaagacacaa attacccgcc gtactatgtt gttgtttacc aatagcattg
      781 atgtgttacg cactgtagaa ctaggtgtat cgattgacaa attgaatgtc ggcggaatgc
      841 gttatcaagc taggcgtgag cagttaagta aagcggtttc agttacgcca gaagagcgtc
      901 aagcatttaa aaccttattg gaaaagggtg ttgaagtaac ggtacagatg gtaccgaatg
      961 atgaaaaact taacttacag gagattatct agctatgctg actgcactac ttgttgcctg
     1021 ctgggcaggt atttgtgcct tagatgatat tggcacacag atgatccgcc gtccgttgtt
     1081 aatcgcaccg gtggtcggat tgattatggg ggatttggaa acggcgttga ttattggtgc
     1141 tacgcttgaa gtgatgtgga tggggatcgg caatgttggt gcatattctg caccggatat
     1201 gatttccgga accgctattg gcaccgcact tggtattgct gctggtgaag cttctactgc
     1261 ggttgcactc gcagtaccga cctcactctt ggcacagcag ttactcattc tttaccgctc
     1321 cggcgtatgt gcattgaacc caattgctga acgctgggcg gcaagcggtg attttgacaa
     1381 gattttccgc attcactata tcccaatgtt aattgccttt tctattcgtg ctattccaac
     1441 gttcttagcc gtctattacg gggcggacat tattgcccaa gcggtctctg ctctgccggc
     1501 aaaagtgatt accggattaa atgttgcagg caaaattatt cctgccatcg gtatcggttt
     1561 gctgatgtta atgatgatta agaaagccga actttggacg ttcttaattg ctggctttac
     1621 gttggcggtt tatctcaaat tatcagtatt gccgattacc ttaattgcac taccgcttgc
     1681 gttgatttac gacttagcag taagcaaaaa agctgaagct gcaccaacca aaaccgcaga
     1741 tgaagaggat tatgacctat gagcaataaa attaccaaac aagatttaaa caaagtgttt
     1801 tggcggatgc aggtgctgaa cgtcacaaac aacttccaat cgatgcaggc gattggattt
     1861 ttaagtagct ttattccggt attaaaacga ctttataaag atcgtccaca ggaagaacga
     1921 gcggaagcaa tgaaacgcca cttaaaattt tttaacagcc acgttaattc tgatgcattg
     1981 attttaggaa ttgccgctgc ggtagaagag actaccaaag aggatgaaaa agagacagta
     2041 accgcgatta aaaccggttt aatggggccg ttagcagggc tgggagacag cttgttgaaa
     2101 ttcacctggt taccgatttg cggtagtatc ggggcggcat tggcactaag tggcaatgtg
     2161 ttaggcccga ttttaatgtt cttgttgtat aacagtgtaa acattgcgac gaagtattac
     2221 ggtatccatc taggttacag caaaggggtt gatttaattc acggtgcggg taataacatt
     2281 ttgcaacgac tcggcaatat ggcaaatgtg gttggtttaa tggtgattgg cgcattaatt
     2341 gcctccgttg tgaaagtcaa agtagtttca caaattgccg ctggtgataa tgtgattaaa
     2401 ttccaagaga tgttagataa agtgatgccg ggactactca gcttactttt aactgtcgtg
     2461 atctactgga ttttgaagaa aactaacggc aaatatgctg caacgatgat ccttgctacg
     2521 atggggatct gtatcggctt agtctatgca ggaatactcg gctaatggac agtgtgtatg
     2581 acattattcg gttggaacag ggcagatacc tgttccgcca acagccggct caatcggtac
     2641 agattttctt tctggacaac cctgacagta aggatagcaa atggctggcg gagactgatt
     2701 tggcagagtt tgagctgaaa ttgagcgctt gtgaagcccg tccgttcttc ctcattcaat
     2761 cagcaaacgg agaacagatt gttgctgaac gcaccttacc tgttgccgga atgaataatt
     2821 ttcgggatat ggggggctat gtcgctcatc agggcaagcg agtgaaatgg ggcaagttat
     2881 accgctccga tcatctgcat aatttaagag acgaaggcgt ggcttatctt gataaattgg
     2941 gaattcagac tgttattgac tatcgcagcc cgaatgaagt cgccaaatac ccaaatcccc
     3001 caattaatgg cagagagcaa acttttcggc ttgatccgaa tgcacatact gccgagcttg
     3061 ctgcccaatt tagtgcagac aagcacgatg aagatcgtaa tttggtcaat aaaatcattg
     3121 ctcaaaaagc tgaaggtaat cttatcaatc gctacgacat tgtaatggca caatatcgta
     3181 attttgtcga aaaagcagaa tgccaaaccg cctttgctga aatgttaagg ttggcgacag
     3241 accctgaaaa tgcaccattg gttcaacact gccgtggagg gaaagatcgc actggtttcg
     3301 gtgcaatgtt actgcttggt attttaggcg tgagtaaagc tgatattatt gctgattata
     3361 tgcttactca ttataaccgc cttgctcgta acgaagagaa aatggcgatt taccgtacct
     3421 tcactcaaga tcaagatgtg ttggactacc tattgtcttt aatcgatact caacccgaat
     3481 ttatcgaaca atcgcttaat actattgaaa cccaatatgg tactattgag caatatgccc
     3541 aaagggtatt aggtattact gcgaaggaaa ttgaagcatt acgggcaaat tatttggcgt
     3601 agaacggtag ggtgtaatat tgaacacact acaaatggag aaatttcata tgcgttattt
     3661 tgcaaaacat tggtataaaa ctaccgcttg tctgacaaca attttcacag catttcctct
     3721 ttttgctcaa ccaattgaaa atataccaac ttcccactac gaattagaaa aaatcattat
     3781 atttagccga cacgggttac gttcccctct tacagaaaaa ggcagtgatt tagaaaaatc
     3841 aaccccttat aaatgggcaa attggagtgt accgaccggt catttaaccc ataaagggac
     3901 gctgttggaa acctattttg ggcagtatct tggtcaatgg ctcacacata atgggttatt
     3961 ggacaaggca agctgtgaaa gtgggaagca gattgaggtt tatgctaata gcctgcaacg
     4021 tacaattggc actgcacaag ctttggttgc cggtgggttt tcaggttgta atgtacaagt
     4081 taaacaccac cagcagattg gaaagatgga tccggtgttt aatccaatta ttcgctccga
     4141 tgaccagaca tttgtgcaaa cagcattaag caaaattgac ctcaataaaa cccatcagta
     4201 tttagcgcca tcctatcatt tattaagcga aataatcgat tatgctcatt cagaaaaatg
     4261 tttgcaggaa aaacagtgtg aattttttaa tgatgttggt aaattaagga ttaaaaagga
     4321 taaataacca ggtgtttccg gtacgatgcg attaagtaaa aatattgtcg atgccttatt
     4381 gctgcaatat tatgaaggtt ttcctttaaa agagattgca aataaccagc ttgatagtga
     4441 tcagaaatgg caaacactca ataaaattaa aaacgattac caatttatgc tacgaggaga
     4501 tccatttatt gctaaacata tttcattacc gctattaaaa tatattcagc aagatttaaa
     4561 tagtgaaaat aaaataacac ttttagtcgg acacgattcc aatattatag ccttactttc
     4621 tgcattaaat gttaagcctt ataaattagc acatcaatat gaacaaactc caataggagg
     4681 gaaagtcttt attgaaatct ggaaagaaaa aagtagccaa gcacaaaaag ttaaactgga
     4741 atatgtatat caatctactg accaaattcg taaagcgatt ccactaagcc ttgataaacc
     4801 accgcaacat cacattttga gtttggatta ttgccaagct gaccaatatg gattatgcga
     4861 ttatatggaa tttaaacagt tactggaaaa agtcattgct gatacattgt gagattttga
     4921 caaagtgagt gaaaaaggta gcataaaaaa gtcgggacag ttgccagctg ctccctgtaa
     4981 gatgttgttt acagcactag taatgctact atccccaaaa agctgctgaa aggcagcttt
     5041 tttgttgtat ataaaagtac acctttctac gcttcattct ttttctccaa ttaaacctaa
     5101 tttaggactt ctagtgataa attcggtatc atatcttaat tcactacata tcgtgcaagc
     5161 cttaaaattg aaagggctga cgaaatccta tttaattctg acacacataa gggaatatta
     5221 tgtcgcaaga tattcaagcc gatttaaaaa agatattggc actattaagc cgtaaccgtc
     5281 gatttcaatc attattttta ttactgttaa gaagtgaaca atttattaat agtgatgcac
     5341 tttctacgga gttacagctt tcagtacgaa cggtaaaaaa agagattaag ttattaaaag
     5401 aaaaacttga accttacggc attcagattt tttctcgacc gttttatggc tataaattat
     5461 tgattcagtt tgaagaatta cacacgcaac taaaacagta tttccaaata tcacagccga
     5521 tgacggtaaa taataaattt gagtctcggg taaatgcgat tttacgccga ttactggtga
     5581 gccagcgtgc attgaaagtt gaagattttc agagtgagct ttatttaagt tttaccagtg
     5641 ccttaacaaa agaatttagt caagttaaag ccttgttagg aaaatatgga ttgatactcg
     5701 aagcgaaacc ctatcacgga atgaacattg taggtgaaag ttatcgtaaa ataatgctca
     5761 cagtacgatt atataaatat tttacccagc ttgaacatcc atttggaatt aaagaatttg
     5821 aacagtgttt tgcttgtcct atgactgaaa
//
LOCUS       DANLUA010000150         5779 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLUA010000150 DANLUA010000000
VERSION     DANLUA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(3..1409)
                     /locus_tag="PXB44_002056"
     CDS             complement(3..1409)
                     /locus_tag="PXB44_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL5282501.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
     gene            complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXB44_002057"
     CDS             complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXB44_002057"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL5282502.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXB44_002058"
     CDS             complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXB44_002058"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL5282503.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(3469..4221)
                     /locus_tag="PXB44_002059"
     CDS             complement(3469..4221)
                     /locus_tag="PXB44_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5282504.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
     gene            complement(4447..>5779)
                     /locus_tag="PXB44_002060"
     CDS             complement(4447..>5779)
                     /locus_tag="PXB44_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL5282505.1"
                     /translation="AEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAFANWAQKKVK
                     GKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYILDSTAAPMCV
                     LMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIMVFFVAYFSF
                     DIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMKTGADALAES
                     GASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAYGLGVKMMSG
                     AITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMAFSTGTSWGT
                     FGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSSTGAQCNHMDH
                     VISQLPYALLVATASIIGYLVVGFTQSAVLGFIATAVVMAILILLFKEKKH"
BASE COUNT         1710 a         1319 c         1008 g         1742 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcctt tgctgttttc aagtgagaaa
      421 ttattaatat ggaatttaaa tgattttgct aatagttgta ataataactc gcctgttttt
      481 tcattttcta atgtttgagg attagataat aaaggcgtaa tatcattggt taatttagca
      541 tctaaatcgt atgccatatc catttttaat ttacccattt caacaccctc aatgttgaaa
      601 gtggctgcct ctaaatctcc ggtagaaatt aaacgatcgc cttttaatag attatcgccc
      661 ttcaccacaa catctttaat ttgagataat ttgccatctt tagacttaaa ctcaatagcg
      721 ttaatttttc cactaccttt gcctaaagtt aaattaggat aactttcatt attttctgtt
      781 tgaacatcat aaactatacc ttgaatttga aaatcaaatt cttctgcttt tatcttaaga
      841 ctttctagct caacagtgct atcaacccct tttaattttt ggtcgtagct atattgcact
      901 ttgattggcg tggtttcaat atgacctgtt tcatcactat gtttaatcgg ggaaatatca
      961 aaatgccctt ccacatcgcc cgaatagctg atattagaaa aaccggtgcc aatatgttca
     1021 cccatcaatt ttttaagggc ttcaggagct tgtaatttgc tttccgcact catcattaca
     1081 ggagctaaat taagtttcgc taaacgatta agcggtagcg gaccgtgatg aagtttatca
     1141 ttaccaacaa agtccaattt cttaccatta cccaaatcca cttctaaacg atatttcgca
     1201 ttagagataa agaaatggcg atcgatctgc acatctttaa gctcaatatt cgcaccaaat
     1261 tcgccactca gttgtttaag ttggttatta gcttgaacaa ccaattcttg atatttttct
     1321 tcaacctgct tacctgtgta ccaacttccg ccaactgcaa cagcaccaat taccgctaca
     1381 actgaaagaa caatttttgt taatttcatt ctgtttcctt ttattttcaa aaaaatttaa
     1441 aaaatgaccg cttgtagttt acttcgtagc cgctttcatc acttgtacaa attcggcaag
     1501 ttctttgagc aaattagctt ggtcagctaa attacgttca ataattttca caatcgctga
     1561 gcctgaaatc gccccgtctg ccccaagcct taacgcctct tttacctgtt caggtttggc
     1621 tatgccaaaa ccttgtaaga taggggcagc tccggcttgt tttaaacttt ccactaaatt
     1681 atccaaatta cttgcgtgag cttggttttc agcactagtt acaccggcac gggaaactaa
     1741 ataagtgtag ccttgggtta attgagcaat ccgttcaatg gtgttttgat cagcattcgg
     1801 cgggcaaata aacaccggct gaattccata tttttcagcg gttggcacaa actcttgagc
     1861 agcgaaaaca ggcaaatcag caatcaacac cgcatcaact ccactttgtt ggcaacgttg
     1921 gaaaaatact tctgcggttg gcacaaaaat taaattggca cacagtagta aaccgatagg
     1981 aatgtctgga tatttagctc gaattttggc aatcagctca aaacagctat cagtgctata
     2041 gccaccatct aaagctcgtt tattcgctgc ttgaatcacc ggcccgtcta agagggggtc
     2101 agaaaacgga aagcctagct ctaaagcatc agccccgttt ttaatcatcg tgtcgataat
     2161 ctcaaaactg cgttcaaaac caggatcgca taatgtcaca aacggcacaa aagccccttc
     2221 gttttttgct tttaatgcag taaatagcgt atcaaagcgg ctcattttac tctcctccat
     2281 ttaaaatttt atccacggtg aaaatgtctt tatcgcctcg acccgataaa ttcaccacta
     2341 ataactgctc tttttcaggg ttctgcttaa tcatttttaa cgcataggct aaggcgtggg
     2401 aagattctaa cgccggaata atcccctcat ttttggctaa tgcttggaat gcctctaaag
     2461 cctctgcatc ggtaatcgat acatattcag ctcgccctgt cgcatttaaa taagcgtgtt
     2521 gcggcccgac agaagggaaa tctaagccgg ctgaaatgga gtaagactct tcaatttgcc
     2581 cgtctttatc ctgcataatc ggcgatttca taccgaaata gatccccgtt gtgccgtgtt
     2641 tgagcggtgc accgtgttca ccggtttcaa ttccttttcc tgccggctca acaccgatca
     2701 atttgacgga ttgctcttcg ataaaatctg caaacatacc gatagcattt gagccaccgc
     2761 ctacggcagc aatcaccgca tcaggcaagc ggtcttcttt tgctaaaatt tgacgtttga
     2821 tctcttcgcc aatcattttc tgaaattcac gcaccattgt cgggaacggg tgcgggccgg
     2881 ctgcagtgcc gattaggtaa tgggtagtat cataatttgc cgcccaatcc cgcattgcct
     2941 cgcaacaggc atctttgagc gagcaagagc ctttttgtac cggaatcacc tccgctccca
     3001 ttaaacgcat acggaataca tttggcgatt ggcgttctac atcttttgcg cccatataca
     3061 ccacgcaagg cattcctaac atcgcacacg ctaatgcggt tgccacaccg tgctgtcctg
     3121 ctcctgtttc ggcaataatg cgggttttac ccattcgttt agcgagtaag atttgcccta
     3181 gcacttggtt ggttttatgt gccccaccgt gtaaaagatc ttcacgtttt aaatagagtt
     3241 tggttttact gcctttggtt aaattacgac aaagggttaa agcagtcggg cgaccggcat
     3301 agtttttgag taaatcggca aattcagctt ggaatgttgg gtcttgctga gcatctacaa
     3361 aggcttgttc taattctttt agcaccggca ctaaaatttc cggcacatac ataccaccaa
     3421 attcaccaaa ataaggatta agaagtgtgt ctgacataat atttcctttt aaatatttct
     3481 cgctacattt aaggcagaag aagtttgagc caccggcatc acttcaaccc gattaatatt
     3541 aacgtgttca ggttgctgat tcagccaaag cacaatattt gccacatctt ccgggcgcac
     3601 aaactgcacg ttttcataaa ctttagccgc tttttcatca tcgcctttaa agcgtacatt
     3661 tgaaaattcc gtaccgccac atagccccgg ctcaacattg gaaacacgca ctttagtgcc
     3721 ggctaaatct gcccgcaaat ttaggctaaa ctgtttcacg aaggctttag taccaccata
     3781 tacattaccg cccgggtaag ggtaagtgcc ggcaatcgag ccgatattaa taatatgccc
     3841 acttttccgc tccaccattt caggcaaaac taagcgtgtc atcgttacca atcctttaat
     3901 attggtatcg atcatttgat accaatcgtt caaatctgct ttctgtgccg gttctaagcc
     3961 gagtgcaagg cctgcattgt ttactaataa atcaatttgt tgccacgctt tagggcggct
     4021 attcagtgct ttttccacct catcgggctg ttgaatatca aatgcaagcg gtagaaattt
     4081 atcgccaaat tgtaaatgca gttcttctaa acgctctgcc cgtctgcccg tgccaattac
     4141 tttataaccc gattgaagca ataatttaca aattgccaac ccaaatccag cagttactcc
     4201 tgtgactaat gctattttca tcttttcacc ctcaaattta aaatgtacta ttaaagtagt
     4261 acaaaaaaat taaaattgca agaaataata ttgccataaa agaaaaaagc gtaccaaaag
     4321 gtacgctaat gctaggagga tcttatttta agatttatat aacacaacat tcgatatcca
     4381 tatcttgagt gctattttat ctcatcaata aatctcattc tgtgattccc ttcacatttt
     4441 cggaaattaa tgcttttttt ctttaaaaag caaaatgagt attgccataa caacagctgt
     4501 agcaataaat cccaataccg ctgattgggt aaagccaacc actaagtaac caataataga
     4561 agctgttgca actaataaag cataaggtaa ttgagaaatg acatgatcca tatgattaca
     4621 ttgtgcgcct gttgaagaaa gaatagtggt atcagatact ggagaacagt gatcaccaca
     4681 aaccgcacca gccattactg cagataaaca aggaataatt aatgctggat ctgaatgcac
     4741 tgccattgag gctccaattg gcaacataat accaaatgtt ccccagctag ttcctgttga
     4801 aaacgccatt gctgcggcta acacaaatag aattgcaggt aaaaactctg ctccaatgct
     4861 ttctgcaacc aatcctgata gatatttccc tgtttgcata tcacttacta cgccgtttat
     4921 tgtccacgca aagaagagaa tagtgattgc accagacatc atcttcacac ctaagccata
     4981 agcacggacg taatttttaa aagaaatatt accgcttgta atgatacaaa tagttgcaac
     5041 tataagccct gccacaccac caacaactaa tgaaatgcct accgtagtat tttcaaaagc
     5101 acctagtata gaaaatgaag cacctgattc ggccagtgca tctgcacctg ttttcatcat
     5161 cattgagact gtagcaacaa taagtgcaat aattggcaaa atcaaatcgc gcacgtgtcc
     5221 tttcgtttca atttgctcat catctaattc tgctaaatgc gctgcttgac gttcaaaacg
     5281 cgccatcgaa ccaatatcaa atgagaaata tgccacaaag aacaccataa tcatcgcaaa
     5341 aatggcgtaa aagttcattg cgctcattgt cataaatgcg cctagtggcg aatagcctgt
     5401 gatggaatgc gtagccaata agccagctat taaggtaata atataagccc cccagcttga
     5461 aattggcatt aacacgcaca ttggagcggc ggtagaatct aaaatataag ctaatttagc
     5521 acgagaaaca cggaatttat ccgtaacagg gcgggcaatc gcacctactg ctaaactgtg
     5581 gaaatagtca tcaataaagg taaagaaaac caaacctgcc gccattaatt ttgcgcctcg
     5641 tttgcctttt acctttttct gcgcccaatt cgcaaacgcc tgattactac ctgaaatact
     5701 taataatgac gttaataccc ctaataaaag caaaaataaa ataatgttaa cattatttga
     5761 atttaatcct tcttcagcc
//
LOCUS       DANLUA010000151         5779 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLUA010000151 DANLUA010000000
VERSION     DANLUA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            22..834
                     /locus_tag="PXB44_002061"
     CDS             22..834
                     /locus_tag="PXB44_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5282506.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAWYTGIHNDRNVYKKLQELALKGNCTKKVTVNYTLDTRK
                     YYGSVFNELLEEPFLLSEYDAYQFTNEVIKFHSEESLKAWGGCLIWEQNNGS"
     gene            966..1664
                     /gene="aphA"
                     /locus_tag="PXB44_002062"
     CDS             966..1664
                     /gene="aphA"
                     /locus_tag="PXB44_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL5282507.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1845..2606
                     /gene="udp"
                     /locus_tag="PXB44_002063"
     CDS             1845..2606
                     /gene="udp"
                     /locus_tag="PXB44_002063"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL5282508.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
     gene            2681..3266
                     /locus_tag="PXB44_002064"
                     /pseudo
     CDS             2681..3266
                     /locus_tag="PXB44_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3277..3762
                     /locus_tag="PXB44_002065"
     CDS             3277..3762
                     /locus_tag="PXB44_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL5282509.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(3800..4669)
                     /locus_tag="PXB44_002066"
     CDS             complement(3800..4669)
                     /locus_tag="PXB44_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5282510.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            4799..5590
                     /locus_tag="PXB44_002067"
     CDS             4799..5590
                     /locus_tag="PXB44_002067"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL5282511.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT         1793 a         1011 c         1202 g         1773 t
ORIGIN      
        1 gtagctgtta tcagttcaac aattggtcgt cctgaattag aaagagctgt tttaagcgta
       61 caaaatcaga cttatccttg taaacattat gtttttgttg atggagagaa atatcaccaa
      121 caagcggctg atattttaaa gaattttcca agtgttattg ttacttatct cccaatgaat
      181 accggggctg atggttgggt aaatagcagc attaatgcaa ttgcaccatt tttagtgaaa
      241 gaagatatta tttgttatct ggatgatgat aattggtttg aacctactca cgttgaatct
      301 ggtgtgcatg ctttattaga aatgcaatct gattatgctt actctttacg taatttttat
      361 gatttagatg gcgagttttt gtgtattgat acgattgaat cgttgggaga atataattgc
      421 gtttatcaag aaccattaga atgtaattat caattaggtg gtgttaaagg tgttttatca
      481 ataaatctga atgattgtca gcatattgat acgaattgct attttattcg tagtgagata
      541 gctcgcctta tcagtacggc ttggtacaca ggtattcata atgatagaaa tgtatataaa
      601 aaattacaag aattggcact aaaaggaaat tgtacaaaga aagttacggt taattatact
      661 cttgatacac gtaaatatta tggatcagta tttaatgaat tattagagga gccattttta
      721 ttaagcgaat atgatgccta tcaatttacg aatgaggtga ttaaatttca ttcagaagaa
      781 agccttaaag cttggggagg ttgcttgatt tgggaacaga acaatggctc ataagcgtta
      841 agcgtaatcg tttgcttgtg atcatattca caaaataagg tttttgtatt agctaagagt
      901 gcctaataag attaaaatag gtatggttaa tcatatggag gtattttata aaatttacat
      961 taaaaatgac ggcagcgaca atgtttgcat ctcttattgc tttttcagca caagcaggcg
     1021 gaccaaaagt agcttatacc cagccgggaa ttgatatgcg aacacatgaa gcacagcaag
     1081 cgccaattca ttgggtatct attgagcaaa ttaaagaaag catcaaagat ttacccccta
     1141 tggcggtaag ttttgatatt gatgatactg ttgctgcaac tagcggttgc ttccactacg
     1201 gaaagcaaaa atattcgcca gacgatttca gctacttaaa aaatcaagat ttttgggacg
     1261 aaattaatgc tggctgtgat aaatattctt tgccaaagca agttgctatt aatttaataa
     1321 aaatgcacca agaacgtggt gatcaagttt atttaattac ggggcgtacc gcaggtaaaa
     1381 atgaacaagt tacaacgatt atgcaaaagt atcttggtat taaaaatatg caaccggtaa
     1441 attttatggg ggggaaagca cattcaacaa aatatgataa aacccctgcg attattgaac
     1501 ataaggtgca aattcactat ggtgacagtg atgatgatat cttagctgcc cgtgaagccg
     1561 gtattcgagg tattcgtata cttcgtgcag caaattctaa ttatacgcca ttccctcagg
     1621 ccggaggata tggtgaagaa gttgttgtca attcaagtta ttaatttctt agtctgaaat
     1681 atggccacca ttgcgtggcc atcatttttt atttatataa tgacgaaacc gtttaccttt
     1741 gatctagttc acaaaatcaa actttgcttg caaaaaattg tcgtaaatta accgcttgct
     1801 aagatagaat ggctcatatc cagttagata aaaaggagat aaaaatgtct gaagtatttc
     1861 atttaggttt aaccaaagcc atgctcaaag gtgcaaaaat tgcaatcgtt ccgggcgatc
     1921 cggctagaag tgaacgtatt gccaaaaaaa tggacaaccc agagttttta gcctcaaccc
     1981 gtgaatttac ctcttggtta ggttatttag atggcgaacc ggtggtggtt tgttcaaccg
     2041 gtattggcgg accgtcggtg tcgatttgtg tggaagagtt agcacagtta ggtgttcgca
     2101 cattcttacg cattggcaca acaggtgcaa ttcaagagca tattaatgta ggcgatattt
     2161 taattaccac cggagcggtg cgtttagatg gtgcgagcca acattttgca ccaatggaat
     2221 atcctgcagt agcgaatttt gaatgtacta atgctcttta tgcagcagca gtggaatcag
     2281 gcgttacacc ttatgtgggg attacagctt catcagacac tttctacccg gggcaagaac
     2341 gttacgacac tttcaccggc aagatctatc gtcatttcca aggttcatta aaacagtggc
     2401 aagatctcaa tgttatgaat tttgaaatgg aatccgccac tctctttacg atgtgttcgg
     2461 cattaggctt gcgagcaggt atggttgctg gtgtaattgt aaaccgcact caacaagaga
     2521 ttccgaatga agcaaccatt cacgacaccg aacataaagc cgtttcggta gtagtggaag
     2581 cggcaagaaa attaattaaa ggctaatctt taaatttaaa agcaaaaaag gtaaattgtc
     2641 tttgttacaa tttacctttt ttctttttag ggagctttct atgaaaaaat ggttgatgtt
     2701 aattacatta attttcggca ttaccgcttg tttttccagt ttagaatttg atgaaagtaa
     2761 acaagaaaaa atcaattcat tcttgattgt tggtaataaa ttatatgtgg tgggtgaact
     2821 gcacgattat gaatttgagc atcagcaagt tgcggaactg aataattttc tcaaaagcct
     2881 aattgctaat aaaatcaaat ctgttgaggc aaacttcgag gttaaggaat ccaaaaaagt
     2941 ggaaggtagt tatagaatta ttttagatgg gaaatcactt acagaaagtg aaagaagtac
     3001 ggtgatggtt taattcagct caaaatccat ctattactta tcaggcggaa gggaacttgg
     3061 tgaaattagc caatagagaa gagcttttgc aaaaattccg atttaaacaa gcgcttgagg
     3121 cgaatgtgcg ttattacaaa atgaaaagtg gtcttcaaaa tacggctgaa ggagcgttaa
     3181 gtattgtaat gttgcctgtt gctgtagtag ctgttttacc attctatatg ttttgggttg
     3241 gtgcttgtgc cacttacgga tgttaaataa ctttttatgt attctctata cctgaaaatt
     3301 ttatcggaat gcgattggtg ttgtgatcca aaaacacttt ctacctacca gcaagaatgg
     3361 cttctgcacg aagggtcact tacccaaaaa ttgtatgatg tcgcaaccaa tttcaacgtg
     3421 gaagtagtac aagaaaagtg gattgcaaaa aacagtgaaa aaacgaccgc ttgtaatgaa
     3481 gatgtttggt cgcgagaagt gttactgaaa gatggtgaaa aaaattggat ttttgcccaa
     3541 actcttgtgc caagatcaac aatcgaaaat atcgctcaaa acgtgttgga attgggcaat
     3601 cagccgattg gtttatggct attttctcaa aatcctactc gcaccaaatt agaatggaca
     3661 caaacagaga acggtttatt cgctcggcgt ggggtgtatg aaattagagg gtattctctg
     3721 gaaattcgag agcttttttt agaaaccttt ccttatttat aaaaaaatcg gttggtaatg
     3781 acaccaaccg attttcatct tattttgcca agaactgata aattgcagcc atatccactt
     3841 cgctcaagcc tgaatcattg gcttgattgt aggttttagt ggcttgctca ataagcgggt
     3901 aagatttgcc taatttcgca atttcttcgc ttgccaaatt aaaatctttg gtcatatgtt
     3961 tcatcataaa cgcaggcgga aattcattag cttggtacat cggcactttt gcttggaaga
     4021 tcgggctgtt cataaacgag cctgaaatca tttcgattac tttctctttc ggaatgccat
     4081 attggtcggc gaagtttaac gcctcgccat aggcttgaat gaagatgccc agtagcgagt
     4141 tgatcatcaa tttaatccca ccggctttac ccacttcacc ataatagaaa gtggtggtac
     4201 taaaggcagc gaaaagcggt ttgagcaact caacggtttc ttctttaccg gcagccagta
     4261 ccaatagttt acctgcttct gccactttgc ttgaaccgga aaccggagct tcaacaaatt
     4321 ctgcaccgtg ttgggctagt agagcttcta ccgcctggtt ttggcttggg gaaatggtgc
     4381 tcatattcac cactactttg ccgtttaatt cagccaacac ttcatcgctt aacacctgtt
     4441 gcacggcggc atagtctgca atcatcaaga aaatcacatc gtaggctttc accaattcca
     4501 acacagaagg atataatgtg ccgcctgctt caactacctt ttctgctttt gctgggtcgc
     4561 ggttgtaaac gcccacgttc actccgctat ttagcaaatg ttttgccatc ggttcgccca
     4621 tttgccctaa accaatccaa ccgactgctt gtgtattaat gtttttcatt atttttctcc
     4681 ttactgaata aagcaaattg attattgcga ttttccctca tcaagtcaaa tagagagaac
     4741 ttgaatatag caatggaaaa gagtagcatt ttagcactta cttttttgaa caataccaat
     4801 gaaaccaaca attcttttat acgattccgg aatgggcgga ttgacgattt acgatgaaat
     4861 ccgaaaagtg ttaccgcacg ctcactattt atattgcttt gataacgctt ttttcccata
     4921 ttccgagaag tcggaagcag ttttaattga gcgagcggtc aaaattgtgc aaaaaattgc
     4981 agaaaaacaa ccgcttgata tggttgttgt tgcttgtaat acggcaagca cagtggttct
     5041 gccggcattg cgtgagcatt ttaattttcc gatagtagga actgtaccgg caattaaacc
     5101 ggcagcggca atttccgaaa ctaaagtcat cggcttgctc gccactaaag gcacggttgc
     5161 tcgcccttat gttgatgaat tgataagaaa atatgctcag gattgtacgg tagaaaaaat
     5221 tggcacaacc gatttggttg aaattgttga agaaaaacaa cgaagcggta aagtggatat
     5281 gaaccgacta caaaaagtgg tcgaggcttg gcaaggtaag ccggaattgg atacggttat
     5341 actcggttgt acccattttc ccttagtcaa agctgaatta cagcaattat tgcccgatgt
     5401 gaaatatttt gttgatccgg gtgtaggtat tgccaaacgg gtgctaagcc ttttggaaga
     5461 acgcaaattt caacaaaaag ccgattttga aaatcaagca ttctgcacta aacaaagttc
     5521 tgcatttaat atcaaacaag acgttatgca gaattgggga tttaataatc tttcggtgat
     5581 tgatatttaa acaaaacaat ctgttttgat gaaaaaaggt tcagttgagc aaaaacttta
     5641 aaaaagggct tgcactcaat tttaaaatcc ctataatacg cagcacacaa cgacgcactg
     5701 ttgtgaacga tttaaaattg atttccagtg cgtcgtttct ttttgttctt taacaattta
     5761 tcagacaatc tgtgtgggc
//
LOCUS       DANLUA010000152         5771 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLUA010000152 DANLUA010000000
VERSION     DANLUA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..466)
                     /locus_tag="PXB44_002068"
     CDS             complement(<1..466)
                     /locus_tag="PXB44_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282512.1"
                     /translation="MKQNRKKSKKVIGTHKKENGLKQFIKNNKIPIILISAIWSIFTT
                     DILERIANMPIIWKNFEENYLYNKKLDGLWSTNTNYIIDYKLLNIPEEQPNVIIEFMV
                     NKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLIFDRELKLSYLVSGG"
     gene            complement(711..1304)
                     /locus_tag="PXB44_002069"
     CDS             complement(711..1304)
                     /locus_tag="PXB44_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282513.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(1294..1464)
                     /locus_tag="PXB44_002070"
     CDS             complement(1294..1464)
                     /locus_tag="PXB44_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282514.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(1466..2208)
                     /locus_tag="PXB44_002071"
                     /pseudo
     CDS             complement(1466..2208)
                     /locus_tag="PXB44_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(2205..2648)
                     /locus_tag="PXB44_002072"
     CDS             complement(2205..2648)
                     /locus_tag="PXB44_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5282515.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(2642..3079)
                     /locus_tag="PXB44_002073"
     CDS             complement(2642..3079)
                     /locus_tag="PXB44_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL5282516.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            3318..3761
                     /gene="lysM"
                     /locus_tag="PXB44_002074"
     CDS             3318..3761
                     /gene="lysM"
                     /locus_tag="PXB44_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL5282517.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(3815..4666)
                     /locus_tag="PXB44_002075"
     CDS             complement(3815..4666)
                     /locus_tag="PXB44_002075"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL5282518.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            4759..5310
                     /locus_tag="PXB44_002076"
     CDS             4759..5310
                     /locus_tag="PXB44_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282519.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            5519..5613
                     /locus_tag="PXB44_002077"
     tRNA            5519..5613
                     /locus_tag="PXB44_002077"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5554..5556,aa:Ser,seq:gct)
     gene            5627..5703
                     /locus_tag="PXB44_002078"
     tRNA            5627..5703
                     /locus_tag="PXB44_002078"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5661..5663,aa:Arg,seq:acg)
BASE COUNT         1705 a         1095 c         1111 g         1860 t
ORIGIN      
        1 atcccccact aacaagataa ctcaatttca actctctatc gaaaataaga tttaataatg
       61 ttggcttagg gctttccaat ctaaaactcc aagttagagg tattaactca cacagagctg
      121 ttgaagtcat ttcccctaaa atcgaattgt catctttatt aaccataaat tcaatgataa
      181 catttggttg ctcttcaggt atgttcaata atttatagtc gataatgtaa tttgtatttg
      241 tagaccataa accatccagt tttttattat ataggtaatt ctcctcgaaa tttttccaaa
      301 taataggcat gtttgcgatg cgttctaaaa tatcagttgt gaagatactc catatggcag
      361 atataagaat aataggaatt ttgttatttt ttatgaactg ttttaagcca ttctcttttt
      421 tatgagtacc aataactttt ttagactttt tcctattctg tttcatcttt gtgtttttgt
      481 tccaatatcc ccgatggtga tcgtcttcga cgagagctta tttatttaag aacctatcag
      541 agataggcat aagcggtctg tttcgagaag ttttttgcaa agtttttctt taatccgaca
      601 gcttgtcacc cacacacaag ccgtgtgggg ttacgcatag ctgagccact ttgtggctct
      661 aagttacagc tccaaaggag ctgggattgt cgagcctagc acggctcgtg ctaggtggtt
      721 ttaaacccta attccactaa ttcatcacaa agctgattac accaattcgg tattttgtct
      781 gaaattttta tcactttctt atcagcaatc tgcaaaatca aattcggctt ggtttctgaa
      841 ttatcataaa tcagtgtttc atcattgata aaaatcgctt caattaaatt ttcacggcta
      901 atgtgatagc gttttctaat ggtttcttca tcaataaaat gtccgccggc tttcactctt
      961 gcttttaccc gtgcgatgtt aattttatag ctatcgacac caatataatt taatcgagtt
     1021 ttgaaatatc ctgctttggc attttccatt cgttttagaa tagatttacc cgagagggtt
     1081 gattccattg aaaatgcaac ctgatttttt actgcaaact gaaatagttc gattgctttt
     1141 ctgcccgcct caatatcggc caatcttgga ttgagtgggt taatctgcat cgcaatatta
     1201 tcggaatcaa tcacaatctc gactttatct tgattgaaag aacgtaaagt agatttgccg
     1261 gctccgtttg tgccacagta aaaaattgct aggttattgt gcatattcaa tcactttttc
     1321 cgtaccgtcc ggataatgct gaattaaatc cccattttct cggcgatacg tcagcacttc
     1381 gtgctttttc ttttcttcaa accatgctgt aaagagaata tccagttcac gttgtttttt
     1441 cagtaattct tcacgacttg tcattttatg tcctctgttt cagaatcgcc aaataccttt
     1501 ccaactcagc taattcatac tgactaatca attcaaccaa actactaata tcttgttgat
     1561 ttttatggaa atcatcaaaa cattggtaat attgggctcg attggtaaat ttaatatcaa
     1621 tcggtaaata gccctgtttt aataattcaa aattaatcag taatcgccct gtgagcccgt
     1681 ttccatcaat aaaagggtga atgctttcaa aggctaaatg cagccaactg attgcctcta
     1741 tgggatgaag tgcggtcagt ttttgttgat atttttcaat caataattgc atttcttcgg
     1801 caattaaata aggggggggg cattttctgc ccctaaaatt cgtactggaa ttttgcgata
     1861 aacccctcgg ctttccgcat tgcccattaa taccagagaa tgaatatctt taatcgctcg
     1921 ttcgcttaat ggttctttat tggcgactaa ttcataaata tagttgaatg catccttaaa
     1981 accgataata tctaaatgct cccgaatcgg tttttcggca atggtcattc cctcttttaa
     2041 aatgagcacc gtttcacgca aagtaatggt attgccttca attgcattgg aattatagct
     2101 gctttcaata ataaactctt cccgcagtcg ttgaacttcg ctttcagata atggacggcg
     2161 ttgtgaaagt gctgttttta attgttcgat tttagctaat ttattcattc taacaccata
     2221 tccaaatgcg caatcccatc ttccaaatac acttctgaaa ccgctttaaa cccaaaactt
     2281 tcataaaacc cttgcaggta actttgggct tgggcgtgaa tgggttgagc aggaaaatgt
     2341 tcgccacaat aatccatcgc tttttgtacc aattcccttg ctaagcctga gccacgattt
     2401 tctttcacca ccaacactct acctatatgc acaccatcat tggctggaat taaacggcag
     2461 taagcggtca gattttttgc attttttgta aagaaatgca ccgcttgcag gtctttatta
     2521 tcgacttctt ggtaagggca gttttgctca accacaaaca ccgctgtacg tgcgtggtaa
     2581 atctcaaaca actcttgggt ggagagttca tcaaagcgtt ttgcgcgcca agagaggttt
     2641 tttagcattg tatcgtttcc agttgccaac caagttggcg gtattgttta tagcggtcac
     2701 gagcctgtgc tttggcggtt tcttccaccg gcacgaaatc aataatctgg gtaaaactgc
     2761 cgataaattc ggggagttgg gtttggagtg aaatcaacaa atcccgacgt tgagcattgc
     2821 gtttaccttt ccacgaaatt tcgataggcg ttgggtaagt ggtgatttct cccgataaat
     2881 tgtgcggcac gaacgaatcc ggatctcttg cccaaagtgc ttcgtcaata tttaacgctt
     2941 gttgctcggt ttcgcatgca atcagtaccc gtttacctaa tcgccacgct tgagccgcaa
     3001 gttcgcaggc ttgcatttca gcagcggaaa gctcggctgc atcggcttgg cttaatagat
     3061 aaaattgtac ttgtttcaca gaatgttccc agaaattcaa tcagatattt aaacttaata
     3121 ttctagctga aaatgagtaa aatataaaat ctaagcgttt gtttttgcaa aaaattgaga
     3181 aaaaatgacc acttgtgttg cgttgccttg aggaactaat tagcttttag acgttctaag
     3241 agaacaatgc actatttcaa tagttggaaa ttgtgtagaa tacccacttc atttagctat
     3301 tttataagga acttactatg ggcttatttg attttgttgg cgacattggt cgtaaattat
     3361 ttaataaaga agaagatgct tcaaaagcgg ttacagagca tttggcggaa gacaatccgg
     3421 gcgttgaaaa tgtaaacgta acggttgaaa acggggtagc gaaaatttca ggcattgctt
     3481 caaccgcagc agctgttgag aaagcggtat tgatggcggg taatgtggct ggtatcactg
     3541 aggtaaatat tgatgaagtg accttgcctg atgacgtgca acttgcaagt gatgatgagc
     3601 tttatgttat ccaaaaaggg gatacgttgt gggaaatcgc cacaaaagct tatggtaacg
     3661 gtgcaaaata taaagcgatt gtagaagcaa ataaagaagt tattaaagac gaaaataaaa
     3721 tcttcccagg gcaaaaaatt cgtatcccga aaggtttata atcaaattga aagagctaag
     3781 ctggtcgaaa gtttagctct ttttgtaggt ggtgttagac tgaaaatgga tattgaatgg
     3841 cagggtggta ttcataacct tcaactttga aatcatctaa gctcacccaa gtttctaaat
     3901 cttctaagga ttttatatca ggattaatga ttaatttagg tgcattgagt ggttctcgct
     3961 taagttgaac atcacgcatt aattctagtt gatcttcata aatatgcgca ttgacaattt
     4021 tatggaacgc ttgtcctgcc ttatgccctg taatttgagc cattaaagct aaaaaggtat
     4081 agcattgaat catattccaa tttaacccaa gcggtacatc tgccgaacgc tgagtactat
     4141 tcaggtaaag cgtgccatca agtagagaaa aatgatggct gtgtaaacaa ggacgtaagc
     4201 agcccatatg gaaagcgccg gggtggtaga aggtataaat ttcaccacgg ttatcaattc
     4261 cttttttcaa atcgtccaca atttgtcgca ataaatcgac tgagccaccg tctggtttcg
     4321 ggaaattacg ccctactgcg ccgtaaacca agcccatatc atcttcccct tttcgatagg
     4381 gattagcaag ccattgtgca ttttcatttg cattagcgtc ccacgatttt gtgcctaatt
     4441 gacggaaatc agcagcatta ctatagcctc gaatatagcc taaaagttcg gcaatcgcag
     4501 ctttccaata actacggcga gtggtaacta aaggaaattc tcctttttct acgtcataag
     4561 ttaagtcagc attaatgact gtgaggcagt gtttgcctgt acgttcattt gcaacccatt
     4621 tgccttcgct aacgatgcgg ttacataagt ctaaatactg tttcattatt tacctcataa
     4681 aaaattttag gtaattttgc catttattcg gtaaaatagc taaaacttta tgacacaaca
     4741 caataaggag ttatggatat gaaaggtata atgaaatcca ttctggtttt ttccaccgct
     4801 gtttttttag cagcttgttc atcgaataaa agcggttgga cacgtttaaa tgataatgat
     4861 gttgatcaga aatcttatgc tattgcttat ggtgcaacgg ctcagactta tgcggataga
     4921 gtaaatgaaa gctatgatat caattccttt atgaatgggg taaatgattg gtacactaaa
     4981 aaggttacat tgccaattga gcaaattaga gcaataacga ttaatcgaat gttagaccat
     5041 aatgtgtatg cttattatag tggtgtgatt tatgccgcag atttgcaggc aaatttcaat
     5101 catttagatc ctaattgttg ggggcttgtt caaccagcga gtatgaccca gggcattcac
     5161 gatgcgatgt tagatttaca gaagaacaaa gttcgtggag atgagtatat caaaaatggg
     5221 gttgagcaaa tattgcacct ttgtgtaaaa acgattgctg aagatgaaaa tgaatcgcaa
     5281 tctaaaggta aaaccaaaaa gcataaataa gttaagctat gacaagcggt aaaattttta
     5341 aaaaatttta ccgctttttt ataaccgtat gaaatgttta atatttattc tatgaaactt
     5401 aaaaaaacag cgttttgttt attcaatagg caaacactcg ggatttttca attttcagga
     5461 aaaagaattt gacatctttt taaattctag taatatggcg cccacagaca cagtgagtgg
     5521 tgagatggcc gagcaggctg aaggcgctcc cctgctaagg gagtataggg ttaaaaagct
     5581 ctatcgaggg ttcgaatccc tctctcaccg ccatttactt tatcacgcac ccgtagctca
     5641 gctggataga gtactcggct acgaaccgag cggtcagagg ttcgaatcct ctcgggtgcg
     5701 ccattttaat ttttactcta ttcaaattaa aatggttgct gataaaatta aataaactta
     5761 caattcaaat a
//
LOCUS       DANLUA010000153         5750 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLUA010000153 DANLUA010000000
VERSION     DANLUA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(23..1660)
                     /gene="ubiB"
                     /locus_tag="PXB44_002079"
     CDS             complement(23..1660)
                     /gene="ubiB"
                     /locus_tag="PXB44_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL5282520.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            complement(1734..2711)
                     /locus_tag="PXB44_002080"
     CDS             complement(1734..2711)
                     /locus_tag="PXB44_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5282521.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(2872..3519)
                     /locus_tag="PXB44_002081"
     CDS             complement(2872..3519)
                     /locus_tag="PXB44_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL5282522.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(3710..4462)
                     /gene="ubiE"
                     /locus_tag="PXB44_002082"
     CDS             complement(3710..4462)
                     /gene="ubiE"
                     /locus_tag="PXB44_002082"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL5282523.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            4807..>5750
                     /gene="corA"
                     /locus_tag="PXB44_002083"
     CDS             4807..>5750
                     /gene="corA"
                     /locus_tag="PXB44_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL5282524.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKG"
BASE COUNT         1614 a         1283 c         1111 g         1742 t
ORIGIN      
        1 aaaatttgtc aaaaacaacc gcttatttgc gttgactcaa aatcaatacc agtggaaata
       61 aagttaataa cggcacactt agccatatcg gcaaagattc aaatttccaa aatgcgccac
      121 agatcacaat cgctgaaatt gacagcacaa gaaaacggct atttgcgttt gaattctgtg
      181 cttttaaact ttcgttaatt tccgctaaac ggaaattaat ttgcttttgc tcttgtaagg
      241 ctttaaacaa tgcctccgga aactccgcaa aatgctcccg atattgcggt aaacgttgct
      301 taatgtctcg aaccatcgct ttcacaccta cctgctcatt cagccagttt tgtaaaaacg
      361 gttttgcggt ttgccataaa tccaactgtg gataaacctg cctgcccaaa ccttcaatat
      421 aaagaagtgt tttttgcaac aacaccaact gcggctgcac ttccatatta aattctcttg
      481 ccacattaaa caggttgagc aaaacgtgtc cgaatgaaat ttccgataac ggcttagcaa
      541 aaatcggctc acatacctca cgaaaggctt gctcaaattt atcaatatcg gtatccggcg
      601 gcgtccagcc cgattccacg tgcattaagg caacacggcg gtaatctcgg ttgaaaaatg
      661 cgacaaaact ttcggctaaa tagcgtttgt cattgtggtt caacgtgccg acaatcccgc
      721 agtcaatgcc gatatattgc ggattttccg ggtgttctct gttcacaaaa atattgccgg
      781 cgtgcatatc ggcgtggaaa aaactgtcac ggaatacttg ggtaaaaaat acctgcacac
      841 cacgctctgc cagcaatttc atatcggtgc tgttcgcttc caactcggca atgttagaca
      901 ctggaatgcc ataaatccgc tccatcgcaa tcaagttttt atggcaaaaa tcttgataca
      961 tttcaggcac atacagcatt tcgctatttt caaaattcgc ccgaagccga atggcattgt
     1021 gcatttcttt gcgtaaatcc agctcgtcta aaatagtttt ttcgtactct cgcaccactt
     1081 caaccgcccg tagccgtttg ccgtcattcg ataatttcgg gataaagtga gcaatttcat
     1141 acatcaactc taaatccgcc ttaatcaccg gctcaatgcc cgggcgaatc acttttaaca
     1201 ccacatcttt gcctgcaagc ggttgatttt ggttaaattt tgccgtgtga acctgtgcaa
     1261 tcgaagcaga agctaaggca gtttcatcaa agttttcaaa ccaagtttca agctttccgc
     1321 ccaaagcctg ctcgataatt tgacgagcga ttttgccgtc aaacggttca acattatctt
     1381 gtagcaaggc gagctggtcg gcaagctccg gtgcaaacag atcacgtctg gtagctaaca
     1441 tttgcccgag cttgatccaa accggtccaa gctcttgcaa agccaaacgt aaacgtacac
     1501 cgtaaggttt gtctgaatgt tgattcttca cccaaaacaa agccttacgc cctagcttta
     1561 ttttgcgggt aattggcaga ttcggaatgg cttcatcaat accatagcga agaaaagtgc
     1621 ggataatatg gtaaaagcgg ttgagatttt tgatgttcat tgaaatatat atttaatggt
     1681 aattaggaag attttagcaa aattctgtaa aaatttaacc gcttgttggg ctactatggg
     1741 tatttctgct caaaattctc aatttctttc tgtacttccg caataagtgt ttgttcagag
     1801 gggaggtaaa gttggtattg gctggcaaaa attttattct gattttccgg tagcgaaaat
     1861 tttaccaccg catcattttt atcggcacaa agcaagacac caatagtggc attttcgtgc
     1921 ggcagttttt caactcggtc aaaataattg acgtacatct gtaattgccc caaatcttga
     1981 tgggtgagct tgtgggtttt aatttcaaaa atcacaaaac attgaagtaa acggttgtaa
     2041 caaaccaaat cgacaaaaaa atcatcaccg tctaggtgca aacgcttttg gcgtgcgata
     2101 aacgagaaac cgttacccaa ttctagtaga aaatcctgca aatgcgtaat gatggcttgc
     2161 tctaaatcct tttcgtaata agcactttcc cgttttagtc cgagaaattc caataccatc
     2221 gggtctttaa tgagctgttt cggatcaacc ggctgctgct catttctggc aacagcaagc
     2281 acactttcct tatcattact gagcaataat cgctcgtata gactactatt aatttgccgt
     2341 tccagctggc gtgaactcca attattctta caactttcag ccaaataaaa ttcccttttg
     2401 tcagaatcgg agatttgaat tagacgacga tattgtgtcc aattcaattg cgaccgcagt
     2461 gcggtcgcaa ttggaaacgc acgataaaac tggcgataac gctctaattg acggctacaa
     2521 aaaccacttc caaattctgg ctcaagctct tttgcgagca ttttaatcag gtatgttcca
     2581 taatcagcac gctcttgccc ttgttgctcc tgttcaaaaa tccgttttcc gatgtgccaa
     2641 tacatcagca cacgttggaa atctaccgca cgaaccgcat tttcacgagc ttgagcaata
     2701 atttgtttaa tatcttagat catcgttgtt tctattaaca taaaagcctc cgttttggat
     2761 taagtttatg cttaatctat ccggaggcaa tttctttcac tcaacttttc gagtcatatc
     2821 acaattttat tttgttaaat ttaattttgc acaaacgctt gcgtttgaac atcagctttg
     2881 ctgatggctc gaagagcgaa cgtagtgagt aaatttttgc tctaatttga gcgcttgttg
     2941 ggcaagttct tcgacttggt catagaaatg caccgcctgt aaacgatgca ccaacaccgg
     3001 tctttcttgg attaaattct caaccaaatg ttgattattt tgctcaaatt gagcttttac
     3061 tttacctagc aattttttag caagatcggt gctagtttga gccactacat cgccaacaaa
     3121 aggggaaagc agttcggcag ggtctttttc gagctgttca agtaatgcag tgaaatgttg
     3181 caacacttga atatcgcccg tcagcaccaa cgatttatta ttaatcagct cggttaattt
     3241 ttgcttatcc gctagtttcg gcagggtgtc aaatgccagt tgcaccgtac aatctgcttc
     3301 cccctcgtag ttgccaagcc aatcggttcg gctttcgcta aacaacacaa aaaagtcgat
     3361 tttaggcgaa gttagggcga tttttagcac ttttccggac aatttacgca aattcggctc
     3421 aatatgaggc gagcgtttaa gtaacgcatt aaaagcggtt tcaatcagac caaaggtaaa
     3481 ttgcgggagc attaattgtt gttttacatt atttaacatt cgtacctact taccctcttc
     3541 acttttggga gaaggataga ttgttcttca ttaagaagca aaatcaggga gaggggtatt
     3601 tgcaaaatat tgaataaata tgaccgcttg ccccctctct ctgcttattt cgctaaacgc
     3661 tcataagcgg tctctctccc acaaagggag agagtcattt aagagaaaac taaaatttat
     3721 aacctctgtg taatgccaca atgccggcac ttaagttgta gtaattcacg ctttcaaagc
     3781 cagtttgttc catcattgct tttagctcgt cttgtttcgg gtgcattcgg atcgattcag
     3841 ctaaatagcg gtaactttcg gcgtcattta ccaccacttc gcccactttc ggcaaaatat
     3901 tgaatgagta gaaattataa agctggctga ttgggtcgat aatcggtttt gagaattcca
     3961 acacaagtaa gcgaccgccc ggttttagca cgcggaacat tgaacgtagg gctttgtctt
     4021 tgtcggtaac attacgcaag ccaaaactaa tcacaataca atcaaaagtg ttatcagcaa
     4081 aaggcaagca ttcggcattc gcttgaacgt agctcacatt tccaaccacg cctaaattgc
     4141 gtaatttttc ccgcccgact tccaacattg agctgttaat atcggctaaa atcacctcac
     4201 cactttcacc aacaatgcga gagaattttg cggtaaaatc acccgtgccg ccggcaagat
     4261 ccagcacttt ttgcccttta cgcacgccac tgcaatcaat ggtaaagcgt ttccacactc
     4321 gatgaatgcc gaaagaaagc aaatcgttca ttaggtcata tttgcctgcc accgagtgaa
     4381 acacatttgc gaccagttgc tgtttttctt ctttcgctac ggttttaaac ccaaagtggg
     4441 tggtttctgc cgattgttgc acttcttgtt gcacttcatc tttaggtgct aattgttcta
     4501 catcgctcat tttcttgtct ctagttgcaa aatttaggcg taaccatagc aaaaaagcgg
     4561 tcgtttttct ttaacttttt gcaaattttc ttaagtaaat aaccgctttc ttgactagaa
     4621 aagccaaatt ctgtaacatt cgcacactat tttttcttga ggattacaca ttttggacag
     4681 tcatagtcga tacttacccg tttaacctat cttgtctaac ctcaatgtta ccgacaaggt
     4741 cggtaatgct cgcctagtat ctttgttcct ttttgttcca agcctcctaa ctatttggag
     4801 ctaaacatga tccgtgcttt tggactagat aacgcatgtt taatcagcgt tgatgaaact
     4861 aacccaactt tattaaatga tgcgatttgg attgatctca tcgatccatc agaagctgaa
     4921 cgaaccgtat tagaaaaccg cttagaccaa acactcgctg aagaacgtga attggaagac
     4981 ttagaggcgt ctgcccgttt ctttgaagat gaagacggtt tgcacctgca ctcgttcttt
     5041 tactgcttag atgatgacga ttacgccgat attgccactg tcgccttcac tattcgtgac
     5101 gggcgtttgt ttaccttgcg tgatcgggat ttacccgcat ttcgcttgta tcgaatgcgt
     5161 tcccgccgtg agaaattgat tgacagcaac gcctatgaac tcttactcga tttatttgag
     5221 accaaaatcg agcagcttgc agacgtaatt gaagaagtct atgctgactt agaaaaatta
     5281 agtcgggtta ttctaaacgg tcagcaagaa aatgagaatt tcaatgatgc cctttctcgc
     5341 ttaactgaat ttgaagatgc aagttcaaaa gtgcgtttat gtttgatgga tacgcaacgg
     5401 gcgttaagtt tcttactgcg taaaacccgt ttgccgaata atcagctaga acaagcccgt
     5461 gacattatgc gagatattga atctctgcaa ccgcacaatg aatccttgtt ccaaaaagtg
     5521 aattttttaa tgcaagcggc aatgggttat atcaatattg agcagaaccg aattatgaaa
     5581 ttcttctcgg tggtgtcagt gatgttccta cctgctacgc tggttgcttc tacttacggt
     5641 atgaactttg aatttatgcc ggaacttcat ttccaatatg gctaccctat ggcgattggt
     5701 ttaatgattg ttgccgcctc cgccccttat ctttatttta aacgtaaagg
//
LOCUS       DANLUA010000154         5709 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLUA010000154 DANLUA010000000
VERSION     DANLUA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..707)
                     /locus_tag="PXB44_002084"
     CDS             complement(<1..707)
                     /locus_tag="PXB44_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5282525.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXB44_002085"
     CDS             complement(611..934)
                     /locus_tag="PXB44_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282526.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXB44_002086"
     CDS             complement(1015..1182)
                     /locus_tag="PXB44_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL5282527.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1190..1564)
                     /locus_tag="PXB44_002087"
     CDS             complement(1190..1564)
                     /locus_tag="PXB44_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282528.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1714..1941
                     /locus_tag="PXB44_002088"
                     /pseudo
     CDS             1714..1941
                     /locus_tag="PXB44_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1966..2361
                     /locus_tag="PXB44_002089"
     CDS             1966..2361
                     /locus_tag="PXB44_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282529.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2393..3034
                     /locus_tag="PXB44_002090"
     CDS             2393..3034
                     /locus_tag="PXB44_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282530.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3117..3518
                     /locus_tag="PXB44_002091"
     CDS             3117..3518
                     /locus_tag="PXB44_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282531.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3563..3793
                     /locus_tag="PXB44_002092"
     CDS             3563..3793
                     /locus_tag="PXB44_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5282532.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3861..4127
                     /locus_tag="PXB44_002093"
     CDS             3861..4127
                     /locus_tag="PXB44_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5282533.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4131..4400
                     /locus_tag="PXB44_002094"
     CDS             4131..4400
                     /locus_tag="PXB44_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5282534.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4475..4738
                     /locus_tag="PXB44_002095"
     CDS             4475..4738
                     /locus_tag="PXB44_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282535.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4798..5508
                     /locus_tag="PXB44_002096"
     CDS             4798..5508
                     /locus_tag="PXB44_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5282536.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5486..5709
                     /locus_tag="PXB44_002097"
                     /pseudo
     CDS             5486..5709
                     /locus_tag="PXB44_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         1779 a         1176 c         1104 g         1650 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gacattcctc ttcgttgtag agagcctcta
     1321 atgtgtgcca ttcttttgca agtgcagccc aaactttccc aagttcagcc attttttcaa
     1381 tcctatttcg caaaaaaggg actgtctcca acaacagaaa gcaacgcctg aaatccgata
     1441 catcgtgtgg gtagccttct atttttggtc ttattccgta accaaggtaa aaagccattg
     1501 ttttactgct tacccctgtc tctccattgg caagccacca agcgattttg tcttctatgt
     1561 gcatttttcc tccaaaaatt aaccgcttgc ggaacaagtt ctacaagcgg ttgtcaagtg
     1621 ttcatttgtt caatgaacag ttcattgtga ataacaaaac cgcttgaatt acaagcggtc
     1681 tattttgttg cgtaaattta ctgttagcgt tgagccataa ccgactacga ccgctggccc
     1741 gaagatattg acggcgaggg ggacggcttc tcgcttgcct ccaaacggac gaccactttt
     1801 atgtcagccg gaatgacttt agtggagagt tcgcctggta aagatattgt cgatctcaaa
     1861 catatcccga aaagtagcca cgaagcccca ccgactaccg gtattttaag cctctacaat
     1921 cgaggcgacc gcagacgctt tagcgaaaca gttgataggt aagctatgaa aatccatacc
     1981 caaatccgaa aagaaatcct ttcattatta gaggcaaaat taacggacat cgagcatttt
     2041 tataacgggc aaccgaattt cattgatatt gacgaacagc aacttgccat ctctgtttat
     2101 cttgatgaga ttaaccgtca ggagcttacc ttatgcgatg aacagtggac agctcaactt
     2161 aacatcacga tttatctaaa atcagttgat gaggcagaag atgaattaga tgagtgggcc
     2221 gaaaaaatcc gtgaagtggt tgaaacatat tctgcgtttg aacatttaga gggcatcagc
     2281 ctttctcaat atcaatacga acaagatcaa aatcaaagaa cttggcactc agccacattg
     2341 atttttgatg ttgaatacta acaagcaaat aatccttata ggagaatcta caatggcaaa
     2401 aacaacaaaa gtccaaggca caaaatttag aattggcatt ggacgtgaag cccaaaaggc
     2461 aattacggct atcactttag ctacagcaac gcttactatt gccacatcag gctataaaaa
     2521 aggcgatgca atcgaaatta ccggttgtgg tcagttagac ggcatttatc ctgtattatc
     2581 tgtaacaggc gatcaagtca aattgtgcga agaagtaaac tggacgggta aagatttacc
     2641 ggctaattac accaaagcca aagcagcttt agtacaatac tcagaccaat tctgtgcggt
     2701 aaaaaacatt gaaaaatctg atgatacgtt aagtaccgaa gatgttacaa cggtctgctc
     2761 ggaaggtaca gaaaccgaac ccggagaaat tgaatttggc tcaattaaac tgagcttttt
     2821 tcacaagcca agtacggaaa tgcaaactcg cttacgcaaa ttgttttatg acaaatccac
     2881 ctttgcgtac aaattagaat tacctgataa ccacggcaca acctatggcg aaggtttcat
     2941 tgaggcgggt aacggcttta gtggcgaagt taaaggcaaa tatgaaggct cggtatcaat
     3001 taaaccaagc aaacgtgatt atttgctagt gtaattacaa aatattgctc caaatcgacc
     3061 gcttgtaata ttgcaagcgg tcttttttat cctaaatttt acaaaaggaa tcgacaatga
     3121 cactccgtga aaaactcctc gccaacaaac caaaattaca atctatcgaa atcaacggtg
     3181 agacctacta cctgcgtgaa gccactgttg gcgatatgaa taagcagatt tttgaaaccc
     3241 gaagctggct cattcaacaa gctgaacaag aaaatgttga attaccggca gaagatgatg
     3301 aaacctttga cgaagctctc aaccgttttg gcgaaaaata ccgccttgct caatcggttg
     3361 cctaccgttt atgtgacgaa aacggtgcat tactgtttaa cccacttaac attgacgatc
     3421 tcaatgcgat tgccgaatta gacagtaaag tgattatcga ctttaaccaa gccgtgtccg
     3481 ccccaaaaga ctcagcgagc gaagaaagtt ccaaataacc ctttcgctcg cactgggtaa
     3541 aacccttgaa gaaatcgaac aaatgcctga acgccacttt gccgaatatc agctttttta
     3601 tcaagagcaa ccttttgggc tttggcgtga ggactatcgt gctgctcaac ttgcccactt
     3661 aaccgcaatg atcaaccgtg acccaaaagg caaagcccct gaactctcgg aatttatgcc
     3721 gttttttaat cgggctgatg aagtagaagc aaatgaagat gatggcgttg cggattattt
     3781 ggcgaaaagg taaatctctt gtatttcatt gtaattctgt ctataattgg tgtaatacat
     3841 agaaatacag gagagataaa atggcgacta tcaatgatgc tttcagtttt agaacaaata
     3901 ccgaaataaa aaataccgca tttgatgtaa ttaaaaacta tggaatgaca ccctctcagg
     3961 tgtttaatat gtttttaacc gagattgcaa aaacgaaaac tattccgtta agtttgaatt
     4021 atcaacccaa tcttgaaaca aaattggcaa tgcaagaagc aaaatcaggt aaaaatgaag
     4081 tttatgcctc acttgaagca tttcataaag caatgttagc ggagtaaata atgctacaaa
     4141 tttcgccgac aaacgcatat aaaagagact ttaaaaagat tgcagccgaa ttagtcggta
     4201 gttcggaata tgtggaagta atgtattgcc taataaacca attaccattg gcggaaaaat
     4261 atagagatca cccactacaa ggtgaatggc aaggctttag agattgccat attaagcctg
     4321 atttagtgtt gatttacgct gttgaagata atctgctccg ccttgttcgt ttaggatcgc
     4381 acgctgaatt atttggataa tttctgtaaa aactaaccgc ttgcgatttt gtgctgtggg
     4441 cggtatagtt ggaatattaa caaaggaggg aaaaatgaaa aatgtacttc tatttatcgt
     4501 acactttttc ttaatcgcaa tgctctcaat tatcccaata ttaattattc ttggagcaat
     4561 cgaaagagat ccttactata ctgcgtggat tttccttagc gtatttttag gcattggagc
     4621 attgttatat tttgcaacca ttgtttctaa gttaattaac aagggaaaaa cacaaacatt
     4681 agaagaaaaa cgatatgcag aatattggaa taatgtaaaa gtcaatattc ccaaatagtc
     4741 taactgataa atgaccaaaa gctcgcttta tgcgggcttt tttattggag aaaaacaatg
     4801 agtggcttag gaaaattaac cgttacctta gagcttgaaa atgcaaaatt tcagtccgca
     4861 atgacgaaat ctgattatga ggcacaaaaa tttgccaaga actttatcca aaatatggat
     4921 agagctagaa atcacgccaa agaatttgcc gatcgctcaa cccaatattt aaaaaacatt
     4981 gagcaagcgg caaagaacct caataaaaat tctgaatttt catttttatc gactattggt
     5041 ggacattatc aatcaatgtc tagtggcata ttgagtgcgg caaaagccta tactgatatt
     5101 caaaataaaa tgaaattagt aagcggtagt tcagcagagg cagctaaacg attaactgat
     5161 gtgtttgata ttgcgactaa aacaagccaa agtacagagg ctgtttcagg tgtctatcaa
     5221 acatttgctc aaaatgccca atctctcgga ctggctcaaa aagatgtagc agaattaact
     5281 aaaacggtct ctcaagccgt tgctgcaagc ggcgcaagca gttctgcagc aagtaatgcc
     5341 ctaacccaat ttggtcaatc acttttaatg ggcaaaatga aagcccaaga atttaactcg
     5401 ctcattactc aaaccccaac cattattcaa gcaatggcaa aaggtttggg aatgacaatg
     5461 gcagaattta aagcagcggt ataccgggca aaatctttac ttggataaca ttcaagggca
     5521 aggttttgag agagtggata caccccaaat cggcgatgtg attttaatac aggtcggttc
     5581 tgatgtgccg aatcacgcag cgatttatat tggtgaacaa atggtaatcc accatagccc
     5641 gaaccgatta tctaagcgtg atttatatga cggttattgg ttacgccata cccacagtat
     5701 ttggcggca
//
LOCUS       DANLUA010000155         5636 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLUA010000155 DANLUA010000000
VERSION     DANLUA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            41..730
                     /locus_tag="PXB44_002098"
     CDS             41..730
                     /locus_tag="PXB44_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL5282537.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            740..1642
                     /locus_tag="PXB44_002099"
     CDS             740..1642
                     /locus_tag="PXB44_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL5282538.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            1667..2545
                     /gene="nanA"
                     /locus_tag="PXB44_002100"
     CDS             1667..2545
                     /gene="nanA"
                     /locus_tag="PXB44_002100"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL5282539.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(2606..2812)
                     /locus_tag="PXB44_002101"
     CDS             complement(2606..2812)
                     /locus_tag="PXB44_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282540.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(2770..3408)
                     /locus_tag="PXB44_002102"
     CDS             complement(2770..3408)
                     /locus_tag="PXB44_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282541.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(3650..4369)
                     /locus_tag="PXB44_002103"
     CDS             complement(3650..4369)
                     /locus_tag="PXB44_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5282542.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            4546..4824
                     /locus_tag="PXB44_002104"
     CDS             4546..4824
                     /locus_tag="PXB44_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282543.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            4869..>5636
                     /locus_tag="PXB44_002105"
     CDS             4869..>5636
                     /locus_tag="PXB44_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5282544.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGR"
BASE COUNT         1679 a         1145 c         1238 g         1574 t
ORIGIN      
        1 agtccattga ctaccttttg aacataaaaa aggaactgtt atgtcaacac tatctcgtga
       61 ttctatttta agtcaaattc aaaacgggct gattgcctcc tgccaaccgg ttgatgacgg
      121 ccccatggat aaacctgaaa ttgtcgccgc aatggcagca gcctctgtga ttggtggtgc
      181 taaaggcatt cgcattgaag gtgtcgataa tttaaaagcc gctcgtgcgg cagtgaatgt
      241 gccgattatc ggcattgtaa aacgtgattt gccagattcc cctgtgcgta ttacgccatt
      301 tttagaagat attgatgcct tagccgaagc cggtgccgat attattgcgg tggacggcac
      361 attccgcacc cgcccggtca gcattgaaga ggcggtaaaa cgcattcacg aaaaaggttg
      421 cttggcaatg gcggattgct ccacccttga agaaggttta cattgccaac aattaggctt
      481 tgatattgtg ggtagtacaa tgtctggcta caccggtggc gaaatgcctg atgagccgga
      541 ctatcaatta gtgaaagatt taaaagctgc cggctgtaat gttatggcgg aaggtcgcta
      601 caacacgcct gaactggcaa aaacggcgat tgaaatcggg gcggattacg ttaccgttgg
      661 atcggcattg acccgcttag agcatattgt gagctggttt gccgactctg taaaatcggc
      721 tcaaaaatag gagctgctta tgcgttgttt agcaatagac atcggtggca cgaaaattgc
      781 cacggcaatt gtgcaacaca atcaagtgtc acaacgccaa caaattcaga ccccgactga
      841 taaaccacaa gctgatcagg caggcagttt gcatcaagcg attggtgaga taatgcaaca
      901 ttatcaaggg caatttgatt ttgtggcagt tgcctcaaca ggcattatca ataaaggggt
      961 gcttaccgca ttaaatccta aaaatttagg cggattggca gaatttccgt tgaaacaaag
     1021 cattgaaaaa cataccgata agccgattgc attattaaat gacgtgcaag cggcggtgtg
     1081 tgccgaatat ttaaatgaag accaacaagc ggtcaaaaat tttgtattta ttaccgtttc
     1141 caccggcgta ggcggcggca ttattttaaa cggcgaactg caaatcggca gtaacggcat
     1201 tgcaggacat atcgggcata ctctagccga tccgaacggg cctctctgtg gttgtggacg
     1261 agtcggctgt gtcgaagcag tcgcagcagg gcgtgcaata gaggcagttt ccagccaatg
     1321 ggaaaagcct tgttcaccga aagaggtgtt cgagcgattc cgccaaggca acccacaagc
     1381 ggtcgaatta gtacaaaaat ctgcaaaagc gattgctaat ttaattgcag atttaaaaat
     1441 cagtttagat atacaaaaag tggtgttagg tggcagtgtg ggcttagcgg aagggtattt
     1501 accgcaggtg tccaactatt tagctgaaat gccgagcgta tatcattgcg tattggaaaa
     1561 tgctctatcc gggcaagatg ccggcttaat cggtgcggct tggtgggcgg aaaaccaact
     1621 taatcaaggt tttactcttt aacaaattct aactccggag atccaaatga aaaacttaaa
     1681 aggtattttt agtgcgttac tcgtctcttt caacgaagac ggcaccatca acgaaaaagg
     1741 cttacgccaa attatccgcc acaacatcga caaaatgaaa gtggacggtt tatatgtcgg
     1801 cggttcaacc ggtgaaaact ttatgctttc taccgaagag aaaaaagaaa ttttccgcat
     1861 tgccaaagac gaagcgaaag atgaaatcgc ccttatcgcc caagtcggta gcgtaaactt
     1921 aaaagaggcg gtagaactgg gtaaatatgc cacagaatta ggctacgaca gcttatctgc
     1981 ggtcacaccg ttctactaca aattcagctt cccggaaatc aaaaatttct acgaaaccat
     2041 tattcgtgaa accggcaaca aaatgattgt ttactccatt ccgttcttaa ccggcgtaaa
     2101 catcggtgtg agccaattcg ctgaattatt tgaaaacgaa aaaatcatcg gtgtaaaatt
     2161 cactgccggc gatttctacc tgttagagcg tttacgcaaa gcgttcccga accacttaat
     2221 ctacgcaggt ttcgatgaaa tgatgctacc ggcaacggta ttaggtgtgg acggtgcaat
     2281 cggctcaacc ttcaacgtaa acggtattcg tgcaagacaa atctttgaat tagcccaaca
     2341 aggcaaaatc aaagaagcat ttgaaatcca aaatgtaacc aacgatttaa tcgaaggcat
     2401 tttaggcaac ggtttatacc aaaccatcaa aggcttattg gagttagaag gtgtgcaagc
     2461 aggttactgc cgtgagccga tgacgaaaga acaaactccg gcacaaaaag aagtgaccaa
     2521 acagttaaaa gctaaattct tgtaatttaa gttgcaaaag gctaaacgaa aatcaccgct
     2581 tgtagtaaca agcggtgatt ttttctcaac gacttgcaat taatgaagcc attctgcttt
     2641 ttctaccacg gtgtaacgtg tcaccattat attttaatcg cactttttct tggcgagtat
     2701 tttctgattt caactctcta cctatatcat aaaaccctgt actccagata ttttgagtaa
     2761 tatccgcttt tacaataata tcattgaagc cgttggtctg tttgttatcc atttgcaata
     2821 ccaagtttgt gtttccgttt tggtttcttt atcatctaaa atagaccaaa aagtcatcgg
     2881 gtaatcaaaa acaagtttta acttttcttt ttccggcaca aataacatca aatgatcatt
     2941 tgcagaatac tcaggagcac ttggaccacg agcagcatta ttaaacctca cgccgaaagc
     3001 tcttactttg tcgtttagaa tataacgggc ggtatcaatt ttaagactat ctgcattcac
     3061 atcgatagag gcatcgatct ccatatcctg ctcccacgat tgcaatatcg cattatcatt
     3121 cacacgagca acaaaaagcg tccacttagg tgtgttttcg tcgttttgat aattttcttt
     3181 gctgtattta ggctctatta ccgcataact cactaaggtt tccgttgaat tgttaggagc
     3241 tgttttacag gcagaagcta aagtacgagc agaaagtttc tcttgagaaa tcttaaagta
     3301 atgagcaata atttgatgtt gttgctcagc acaaacctgt gtgttattac cttcatctgc
     3361 ttgagttgca agcgtaagta aaagaactaa gaggagtatc tttttcatat agatttatcc
     3421 gtatagaaag ataactcatt ctatggggta ctgactcttt ttgcatagag agaaagaaaa
     3481 aaatttggtc ggaaaaataa cggcgagcct agcgaaaaca agggttcgcc gtaattttat
     3541 gggggaagct atgtaaaaaa caaaatgagc aggattagaa aaaaatgagc agtttttgcg
     3601 atattttttg cataggtttg atcggtttta aaactatttt aaatctgttt taaactaaag
     3661 agtgtccgat gtggactacc tgcccgatga tttggaggtt ttgagcttct tcggcggtaa
     3721 tctgtattgg gcggtaggac tcgttatcgc taattaattc aatatagtcc cattcttttc
     3781 taatccgttt aacccatacg ctttccccat tctgcacaag ataaatctta tcatcaccac
     3841 gaggctctcg tttgctgaaa tctaccagca tttggtcact gtctgcaatg gtagggtgca
     3901 ttgatgttcc atttgcccaa aacacgccgc agttgtgggc ttttacgccg aggctttgca
     3961 gcaggctgtc ggcgtaaggt tcttgcccgt ctggcttcgt tacgccctcg ttaaagctgc
     4021 caaagcctgc tgaaacgttg attgttctga agctgttaat cattgaaaca atatcatcag
     4081 ccattttttc tagtcgctca aactctcttg ccacaatact ggtattctct gattgtggtt
     4141 tgctttcctt acctgttgcc agccattcaa ggctcacatt tgacacctct gctattttta
     4201 caagatttga acgagatgga tctgcctctc cttttaccca cctagatagt gatgcttggg
     4261 ctacccccac tgctcttgca aactcactgt aattgtcctt aaagctattt tttgcaatcc
     4321 atttcattct ttctgagaag ctctcatcgt aaatgtttgg tttactcatc ttttacgttt
     4381 ccattaataa agttttactc gtaaaacata ctcataaacc attgattttt aagttattta
     4441 tgaataaagc aaaaatattt tagatgtttt tatgttttac ggttgatttg tttaaagttt
     4501 tacggttaaa tacacgcatc aaatagataa aaagaggtgt atcgaatgag tgtattaaac
     4561 gatgttaaaa aaaccgcaca gagagattgg cataaagccg atattcttgc ggaacttcgt
     4621 aaaaaaggtt ggtcacttcg ttctttggct aaagcaggca atgttagcca taacacgctg
     4681 aagtctgttt tagacaagcc ttaccccaaa atggaacgct tagttgcaaa tgccataggg
     4741 gttgcccctg aagtgatttg ggctgggcga acagcagaac gcattgaacg taatcggcga
     4801 ccaactttaa ctaataagtt ttaatcataa atgatcttta acgtaaaaga tataggagaa
     4861 gaacatttat gagcgaatta agtttaaaaa cgcactattc agtggctgaa ttgttaagtt
     4921 ttaagttatc tagcctacca agtgctcata aaaatgtgct tgagaaagca ataagggaaa
     4981 actggcaatc tcaaaaacgc aaaggcagag gcggcggggt tgaatatgaa ttaatcagtt
     5041 tacccaccga agtccaacag gaaatccgca ccaagttact caaacttctt cctgctgaaa
     5101 tcagcaaagg cgaattgagt gtagtgcgac aaaacattga tttggagcag ataaccgata
     5161 aacaactttc tacggcagat gctcgcatta tggtggtgcg gtggttcttg atgcaggaag
     5221 tgcagttggg gctaagccgc acaaaaacgc tagaccaagt cattgcagcg gtggcaagca
     5281 gtgaaattcc tgctgaaatt tgcaaagcga ttatggcggg caatagtaaa gccggtggaa
     5341 agttaaagct atctaaacgc accttgcata gttgggtgct ggcgtatgag gcaggcgaaa
     5401 attcagcgga acggttaaaa cagatgatac cgctgaaaac acaaaaacgt gccgtgcccg
     5461 agcggtgtgg ttggttgcaa gcctttctgc cgttttatca aacctttagc aatgtggcac
     5521 tcacacaggc ttatgctcaa tttgcaatgc agtatgaggg cgaagattta cctacagaga
     5581 gccaagttcg gtatgccttg aagcagttgc ccgattatgt ggtgcaacaa gggcgg
//
LOCUS       DANLUA010000156         5612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLUA010000156 DANLUA010000000
VERSION     DANLUA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            300..1304
                     /locus_tag="PXB44_002106"
     CDS             300..1304
                     /locus_tag="PXB44_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5282545.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1359..3209
                     /locus_tag="PXB44_002107"
     CDS             1359..3209
                     /locus_tag="PXB44_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5282546.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3305..3859)
                     /locus_tag="PXB44_002108"
     CDS             complement(3305..3859)
                     /locus_tag="PXB44_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL5282547.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(3926..5407)
                     /locus_tag="PXB44_002109"
     CDS             complement(3926..5407)
                     /locus_tag="PXB44_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL5282548.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
BASE COUNT         1759 a         1005 c         1193 g         1655 t
ORIGIN      
        1 tatttagaat gtaaacaaac atgagtcgat tttatgttca aagagctgta ataagacgga
       61 ataatgtcag accaagatag atactttcaa gtaaaatcaa gcataaaaat gattttcaga
      121 ctttacaaat ctgaaagaat gtgtaagatc aggattgtcc gaaattcgga tggaaaatcc
      181 cagtggatgt attggctgga tacatcataa taactatgta tttgtgaatg ttatttgaat
      241 gattctacgt agttgattca aaatcagcag tgcggtatct gcatgctaat gatgaaaaaa
      301 tgccgggcga tgcaaataaa attatctcta acggcaccag tgccggagga gcattatcta
      361 ctttgttagg agcttcagct gatcattacg actatgagcc ttatttgaaa gaggcggggg
      421 cattgaatgc cagtgacaaa atttttgcag tttctgccta ttgcccaatt accaaccttg
      481 aaaatgcgga tatggcgtat gaatggcaat ttaatggtgt caatgaatat agccgaatcg
      541 atatgagtcg attaaatgca gcagagttta acgaccgctc aaaaccaaaa ccaaagccga
      601 aaattgaagg ctcgttaaat gaggcggaaa ttaaagtttc taatgaatta gcagaacgct
      661 ttccgactta tttgaacagt ttgcatttgg ttgatgagaa aggcaatcct ttaacacttg
      721 atccaaaagg caacggctcg tttaaagatt atttatctga agtggtaaaa acggctgcaa
      781 ataaagccta tagaggttta gttcaagata gcgaagagca aaaagccttt caacaaatta
      841 gttggctttc atttgagaaa ggcaaagtca gcagtgtgga ctggtttggt tatgtgtttt
      901 cagataaacg gatgaaatca ccacctgcat ttgatgcgtt aaatggaagt tcgggcgaga
      961 ataatttatt tggcacagac acggaaaata accgccattt tacgctttat agtgcggaac
     1021 gttctgccaa taaagatctc aatttagccg atccgcaaat cgtgaaacgg atgaacccga
     1081 tgcactattt ggataaccca aatgctgcgg aacattggcg gattagggtg ggaacggcag
     1141 atagagatac ctcgctggca atttctgcaa tcttagcaat aaaactgcaa atggcaggca
     1201 aaaatgttaa ttatgaaacc ccgtggaatg taccccattc aggtgattat gatgtaaatg
     1261 aattattcct atgggctgat gagctagtca aaggcaaaaa gtaaaaaata gagtaaaata
     1321 aagcagatct gatggtctgc tttttgtttt gaggtcgtat gcaagttaaa aaatttagcc
     1381 aaaaatatat taattgggta ttaaagctag ggcggttaaa agcttctctt ttaggctttt
     1441 ttatggtcgc tctttcggca attttaattc aaagtagctt aacctatctt tttattgggc
     1501 gagtagtcgt ggtggatatt ttacgctcca tcgcttttgg tttaatttct gcgccttttg
     1561 tgctttattt cttcacgtta ttggtggaaa aacttgaaat atctcgcttg aaattagaaa
     1621 aagcagtaag agatttggaa gaactccggg aacaagatgc ctatcttaac gctaaattag
     1681 aaaaaaacag ccgtgataaa agcattttaa tggcgaccat tagtcacgaa cttcgtactc
     1741 cccttaatgg aattatcggc ttgagccaaa ttttgttaga tgataaaacc accgaaaaac
     1801 agcaagaata tctaaaaact atcaatatga gtgccactgc attggctcat atttttggcg
     1861 atattattga tttagaaaaa attgatagta gccgaattga attggtaaga aaagaggtgg
     1921 aatttgaaca actcattcac gatattgcta attttgggca ttttatggcg ggcaataagc
     1981 agattaattt tgaagtgaag tatccgcctg atttgccgaa gtatatctat atcgaccacg
     2041 cacgcttgag ccaaatttta tggaatttga tcagcaatgc ggtgaaattt acccctgcaa
     2101 gcggtcgaat tttgctggaa atttaccaat tagataaaga acagtttagc tttgcggtga
     2161 cggattcagg catcggtatt ccggaaaaag atcaacgcaa aatttttgat atgttctacc
     2221 aagcggaaaa ctctaaagag aaaaaagcgc aaggtagcgg gattggctta tcggtctcga
     2281 aacgcattgc gaagctaatg ggcggtgatt tggtggtggc aagccaagaa aatcaaggct
     2341 caacttttac cctcactgtg caggcagaaa gcacggaaac caagccgata tttgctattc
     2401 agcaacattc ccttaaggtg ctattggtag aagacattga aatgaatgtg gtggttgccc
     2461 gagcaatgtt ggagaaaatc ggttgcgtgg ttgatgtagc aatgagtggt gaagaaacgt
     2521 tccaacattt tgcacagaac agctacgatc tgattttact tgatattcaa ctgccggata
     2581 ccaccggttt tgaaattgca cagcaattaa gattgcaata tgaaaacgag gaaattgact
     2641 atttaccgct gttggttgct ttgacggcaa atgtgatcca aagccggcag gagtaccaag
     2701 cacaaggtat ggacgatgtg ctacacaaac cgctctcttt agatgaatta atgcgttgtc
     2761 tcaaccaaca ttttggagat gattttttgc aaaaaaatga agaaaaacag ccgcttgtag
     2821 agagaggttc tgagaattta ccttatagca ttacaattct taatgaattg attggggtaa
     2881 tggggaaaga gaatgtgctt gccaatatgg atttatttgc tgatttaatg cccacttatt
     2941 tggcaaattt acagcgttat ttaagtgatt ggcaaaccac aaattctgtg gaaagccgta
     3001 aattaacggc ggacgaggct cataagatta agggagcatt agcatcagtc ggattacatc
     3061 agctacaaca aactgctcag cttgctcaat ccgataacgg cgagcagtgg gagcaaaata
     3121 ttgcacactg ggtggaaatg atttgttcca aatggcagat ggatttgcaa ttagctaagg
     3181 aatatgttag aagcaagggg gacatttagt aaatttgaaa tgttacgatg gtgcaagcgt
     3241 ccacgcttgt accaatttta atatctgaag cacaagcgtg gacgcttgtg ctatcaaaac
     3301 gtatttacca tttcttggtt ttcggtttta caaattcaat gcctttatct ttataggtaa
     3361 tatcttccag ctgtaataga aaacgcttat tcggcaagcc accaccgaat ccggttaagg
     3421 cttgattttt ccctaatacc ctatggcaag gcactaaaat cgaaatcggg tttcgcccaa
     3481 ctgcaccgcc gacggctcgc atcgcacttg gcttgcctag ttgggtggca atttcgccat
     3541 aagtagtagt ttgcccgtaa gggatttcac gcaaaatttt ccaaactgat tgctggaatg
     3601 gagtgccttg cggggcaaga aaatccaact gctgaaaatc caatggctcg ccggcaaaat
     3661 agcggtctaa aatttctttg gttttgcaaa aaatttctaa aatctgacca cttgcagctt
     3721 gagcggaaag gaaattatca agcgaggtgg tttgttgctc ttgccctagc tcaatgtaaa
     3781 tcaagccttc ttctgtggct aaaagcaata attgcccgat agggcttggg tagtaatcgt
     3841 agtaaatttg agatttcatg tttttatgcc aataaaaaaa gaggtgtaat tgattttaca
     3901 cctctttaaa ccttaattca tcaaattaga atttgtagtt tacgtttaaa ccgtaaagat
     3961 ttgcacgagc tttgctcttg aatgtggcta aaccgtcttc agagaaggtc aggttgctac
     4021 cacgaagatg tgagtaacct acatcagcag ataggtttgg cgtgaaacgc acggttgcac
     4081 ctacagaata ccaagcacga tcagtgtccg gaattgagat tgatggatga gttacacttg
     4141 cactttcatc aaatgctaca cctgtacgca atgttagcat ttcattcaca tcataagaca
     4201 agcctaatgc aatacgttga gagtcattaa agttctcggt tttgtgcaat aggcggttac
     4261 cttgcatatc ataagcttct aggctttgga atttgctcca gtctgtgcgt ttatagctgt
     4321 attgtgcagc aagtttatcg gttagtttgt ggtaacctga cacttcccag aatgccggta
     4381 ggcgtaatct tagtgtacca tcaatctctg caccgccggt aatggtatct accatcttag
     4441 gaataactac aggaacggta ttagaatatt tgcctttaaa tttaagatca atttcagagt
     4501 ggtaagtaaa acctaaacgg tttctttcat tgatattata aaccaagcca acattccaac
     4561 ccaatgccca tttatcgcct ttaagtttat gaattgttga acttgcattg gtattttgaa
     4621 tgccattaac caaacgttct aattgagcag aattcgtgat ggtttggttt aaattttgag
     4681 attttaataa accgtttact tgagctaata cttgtgtttt ttgagcttca agagcggtat
     4741 tacctgctgc gatacctgcc tcaagagctt ttaaaccttg aattgcactt tgtacttgta
     4801 ctttcttgtc ttccaaaaat tgtttagctg gagcaaaacg tgccgcaccg gcacctaaga
     4861 aacgtcctaa ttctgctttt gcgtgtactg cattcaaacc tgcccctaat acaaaaccat
     4921 aacctaaatc ataagccgca ctaaaattaa cattcacagc acttaatgat gtacgaccac
     4981 ctaagaaacc agcagaatag ttagggtcaa attcagatat caaaccataa tttacattta
     5041 cgccaccacc aatagaaagg cgctcattga taggtgaaac gatataagca tttggaacaa
     5101 tagcggtagg aatgacattt ttatgtgagg catctgcacc tgtagcattg agctttccat
     5161 caatatttac atcagcatct acgaaaatac caccggcaga aatttcggtt tgtttaaata
     5221 acgtcattaa cgcagggttg gttgcaacaa cagaagcgtt atcggctaca gcggcattac
     5281 ctgcgtaagc tctacctaaa ccggatgttg aaatttcagc taattggaat gcggcagcag
     5341 atgcaccggc tgagctgaat gctactaatg aagcgattaa agttttgcta aagtttttca
     5401 ttttcatagg aaatctctta aaaaatatat attttagaat gaataattca taaaagttat
     5461 aagaagaaag tgcagatttt atgtgtcgaa gtactctcta tcaattaaat ttcaactgtt
     5521 ccgatcagtt aggagcaaat aagagtgaaa ttgtgcggtc tatgaactgt ttatctttgg
     5581 tattttggta ttttcggctc ttttttcgta ta
//
LOCUS       DANLUA010000157         5602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLUA010000157 DANLUA010000000
VERSION     DANLUA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(104..619)
                     /locus_tag="PXB44_002110"
     CDS             complement(104..619)
                     /locus_tag="PXB44_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5282549.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GARREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            774..3602
                     /gene="uvrA"
                     /locus_tag="PXB44_002111"
     CDS             774..3602
                     /gene="uvrA"
                     /locus_tag="PXB44_002111"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL5282550.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3694..5427)
                     /gene="dauA"
                     /locus_tag="PXB44_002112"
     CDS             complement(3694..5427)
                     /gene="dauA"
                     /locus_tag="PXB44_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL5282551.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
BASE COUNT         1626 a         1157 c         1309 g         1510 t
ORIGIN      
        1 ccctattatt ttgagtaagg taggggcgct ttatatgagt agctttttat ttcatcgaaa
       61 tgcggtgtat ggaacccatg agctacttaa gtcaatactt tgattagaac ggaatatcat
      121 catcaaaatt gttcattgcc ggctcaggct gtggtgccgg tttagattgc ggtgcagcac
      181 tttgggtttg gttgtaacca taattgctgt tgccttgatt gtagctatta cccgcaggag
      241 cagaatttcc ccaaccgcct tgctgaccgc ccatatcatt accttgacgg ctgccgagca
      301 tttgtagcac atcgccttga atttcggtgg tgtaacgatc ttgcccgttt tggtcttgcc
      361 atttacgggt ttttaaacgt ccttctacgt aaacttgaga gcctttacgt aaatattgcc
      421 cgcatacttc tgcttgacgg cggtagaata caatacgatg ccactcggtt acttcacggc
      481 gtgcaccggt gtttttatcc gtccagcttt ctgaggttgc cacactaata tttgcaaccg
      541 cttcgccatt tggcattgta cgcatttcag ggtcgttacc taaattaccg acaataatta
      601 ctttattgat accagccata aaatcctctg tttgataaat taaactaaaa tttgctaagt
      661 attctacctg aaaatccaaa agatgaacac caaaaaactg atattttgta cagttttatt
      721 aaaatatgcg ataatgatcg aaaattgaga aaatagcact ggacattaaa aatatgcaac
      781 atatcgatat tcgtggagca agaacacaca acttaaaaaa catcaattta accttgccga
      841 gagataaatt cattgtaatt accgggcttt ccggctcggg caaatcgtct ctggcgtttg
      901 atacgctcta tgcggaagga caacgccgtt atgtagaatc actgtctgca tacgcccgcc
      961 agtttctctc cttaatggaa aagcctgatg tggatcatat tgaagggctt tcccctgcga
     1021 tttcgattga gcaaaaatca acctctcaca acccacgttc gaccgtagga acggtaacgg
     1081 aaattcacga ctatttacgt ttattgtttg ctcgagtggg cgagcctcgt tgccctgacc
     1141 acgatgtgcc attggctgct caaactattt cgcagatggt ggatcgagta ttggaagaac
     1201 cggaaggaaa acggttgatg ctacttgccc ctgtggtgaa agagcgtaaa ggcgaacacg
     1261 taaaattatt ggaaaatttg accgctagtg gctatttgcg ggctagaatt gacggtgaaa
     1321 tttgcgattt atccgaccca ccggcattag aattacagaa aaaacacacc attgaagtgg
     1381 tggtggatcg ctttaaagta agggctgatc tagccacccg tttggcggaa tcttttgaaa
     1441 cagcgttaga tttatccggc tctaccgcaa ttattgcaga tatggacgac ccaaatgccg
     1501 aagaaattgt gttttcgtcc agctttgctt gtccgcattg tggttactct ttacacgaat
     1561 tagaaccacg ccttttctcg tttaacaacc cggcaggtgc ttgcccgact tgtgatggtt
     1621 tgggcgttga gcagtttttt gatgccaaaa aagtagtgca aaatgaagag gtttcattag
     1681 ccgccggtgc gatcaaaggc tgggatcgcc gcagtttcta ctattttggc ttattaaaag
     1741 ccgttgccga gcattatggc tttgatttag aaaccccatt cgggcagcta ccgaaaaaat
     1801 atcaaaacat tattttaaac ggcactaaag aagagattga gtttgtttat gtcaatgacc
     1861 gaggcgataa ggtcaaacgt aaacacgctt ttgaaggggt gctgaataat atggctcgcc
     1921 gctataaaga aaccgaatca aactcggtgc gtgaagagtt ggcgaagtat attaataatc
     1981 gtccttgtat ggattgcgaa ggctcacggt tacgccgtga agcccgttat gtattcttag
     2041 ataaaaccaa tttgccgatg gtttcggaaa aaagtatcgg cgaggcatta accttttttg
     2101 aagagctgaa attagccggt cagaaagaaa aaattgctga gaaaatcctc aaagaaattc
     2161 gtgaacggct acagttttta gtgaatgtcg gcttgaatta tctttctctt tcccgctcgg
     2221 cagaaaccct ttcaggtggc gaggctcagc ggattcgcct tgccagccaa attggtgcgg
     2281 gtttagtagg ggtaatgtat gtgctggatg agccttccat cggcttgcac caacgagaca
     2341 atgaacgcct actgaatact ttggtgcatt tacgcaattt aggcaatacc gtgattgtgg
     2401 ttgaacacga tgaagatgcg atccgagccg cagatcatat cgtggatata ggcccgggtg
     2461 ccggtgtaca cggtgggcaa gttatcgcac aaggtacggc agctgaaatt atgcaaattg
     2521 aggaatctat taccggacaa ttcctctcag gcaagcaaaa aattgagatt ccggctcagc
     2581 gtgtgcctta tgagccaact aaattgctca ccttatcggg agcaagtggt aacaacttga
     2641 aaaaagtgaa attagagatt ccggttggtt tatttacttg tatcacgggt gtatcaggtt
     2701 cgggcaaatc aactttagtt aatgacacac tattccctct ggcacagaat gcgttaaacc
     2761 gagcggaaaa cagcgatgtt gctccttatg aatcgattga cggcttatcg cattttgaca
     2821 aggtaattga tattgaccaa agcccgattg ggagaactcc tcgctcaaat ccggcaactt
     2881 acaccggatt atttacgcca attcgtgaga tttttgccgg cacgcaagaa gctcgagcca
     2941 gagggtataa cgtagggcgg tttagcttta atgtgcgggg cggacgttgc gaagcctgtc
     3001 aaggtgacgg cgtgattaaa gttgaaatgc acttcttgcc tgatgtgtat gtgccttgcg
     3061 atcactgtaa aggcaaacgc tataaccgtg aaaccttgga aattcgctat aaaggcaaaa
     3121 ctattcacca agtgttagag atgacggtgg aagaggcgag agaatttttt gcccctatgc
     3181 cgatgattgc ccgcaagttg caaaccttga tggacgtggg gttatcttat atccgtttag
     3241 ggcaatcttc tactacgctc tcaggcggtg aagcccaacg ggtgaaattg gcgaccgagc
     3301 tttccaaacg agacaccggc aaaacgctct atattttaga tgaaccgacc accggcttgc
     3361 attttgccga tattaaacag ttactcggcg tgctacatcg cctgcgtgat caaggcaata
     3421 cgattgtggt gatcgaacat aatttagatg tgattaaaac cgcagactgg attgtcgatc
     3481 ttggccctga aggtggcagt ggcggcggtg aaattattgc taccggcaca ccggaagaag
     3541 tggcaaaaga caaacgttct cacaccgcac gcttcttaaa agagattttg gctaaaggct
     3601 aacgttcaac acaagcggtc atattttcgg aaaatgttgc aatttgcaaa aagttggaaa
     3661 atatgaccgc ttgttttata tccggagtta ttcctagtta gcctgtctga ttttgtcgaa
     3721 ggcagattca agatcgctac tgaattgaat ttgcgggatt tcgtttaccg ttttttgtga
     3781 gcggactagc attctcatcg gctgaaattg cacattacag agatgaagtt gttgatgtgg
     3841 caacatatgc tgtacaaagc gggttagggc gtgaatgcca ccagcatcta atactgtaac
     3901 ggcatcgcat tgcagcacaa tatgtttaat ttggtgatcg gtatgtacgg ttttattgtg
     3961 cagatcggca aagagattat cggcggcagc aaaaaataac ggaccgctga tccgataaac
     4021 caacacatcg ttcaaatcgg ttgggtgagc catttcaaag gatttagtca tttctgcaat
     4081 agtacggata aatagcaggc ttgccagtaa cacccctacc gtaatggcaa ttaccatatc
     4141 aaataatacc gtgagggtaa tgcaagtcag taaaaccgca atttcatttt tacctgaacg
     4201 gcgggtaagt tgaatgattt gtggaatatc tgccatattc caagctacta ccaataatag
     4261 tgcagccatt gaggaaagag gcaaatagga aagtgcttta gccaaaaaga gtagggcaaa
     4321 taaaaccaac aaggcgtgaa ccacactaga aatgggggaa actgccccgg aacgaacatt
     4381 ggcagccgat ctcgcaatcg ctgctgtggc ggtaattccc cctaaaaatg gcgaggcaat
     4441 attacctaag ccttgggcta atagttcgtt gttagagtgg tgcttggtat ctgtcatatt
     4501 atcgcacact accgcacata ataacgattc gatagcccca agcattgcca ttgaaaaggc
     4561 agccggtaat aaggcttgaa tggtggttaa gttccaatct atccgattcc cctctgcatt
     4621 cgggagattc caaggtaaca caaattcagg taatacgctt gggataccat acccagtggt
     4681 gccgtcagag agcgtgtagt ggaatgctga gccgatagtg gcaacgtttt gtccgaaatg
     4741 aatcagcagt aatgataaca gcgtgccgat aaagacggcg ggtaaatgcc cgggaatagg
     4801 caaccgtagc ttgtgccaac gagttaaaac caacaatgtt acgacaccca ctaaggtgtc
     4861 agcccaattt atggttggca atgctgaaaa aatagcctct agcttgccaa gatagtgttc
     4921 aggcattttt tcaatgctta agcccagaaa atctttgatt tgcaaggtgg cgatcacaat
     4981 accgatgcca caggtaaaac ccagtgtgac aggtagagga atgtattcaa ttaagcgtcc
     5041 taatcggagt aacgccatca atactaaaat tcctccggaa agcattgttg ccatgagtaa
     5101 tccacttaag ccaaactgct gcgtgacggg gtatagaatc accacaaaag cggctgttgg
     5161 gcctgaaata ttaaatcttg aaccgcctgt tagggcaatc acaatcccgg caacaatcgc
     5221 cgtatataaa ccgtgctgtg gtggcacacc gctggcaatc gccaacgcca tagataacgg
     5281 aatagcaata acgccaacgg ttaagcctga aataatatct ctgattaaat gttgtttacc
     5341 ataacctgca ttgagagcat cttttaatgc actaaaaggc ttgacggcaa gaaaagcatt
     5401 tttagttaaa aaccatttat ttaacataga atgttcgagt gaagagaatg tttcgcttat
     5461 tttactgcaa tgaataaaat taaatttgag ctaacgcaag aaaaagcagg aaagacgaaa
     5521 tatgttggag acaagcggtt gttttaggcg aaaaatttgc aaatacaaat aattagctga
     5581 ttttattttg aaaatagcgt aa
//
LOCUS       DANLUA010000158         5580 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLUA010000158 DANLUA010000000
VERSION     DANLUA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(9..545)
                     /gene="hpt"
                     /locus_tag="PXB44_002113"
     CDS             complement(9..545)
                     /gene="hpt"
                     /locus_tag="PXB44_002113"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL5282552.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(584..1933)
                     /gene="pmbA"
                     /locus_tag="PXB44_002114"
     CDS             complement(584..1933)
                     /gene="pmbA"
                     /locus_tag="PXB44_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL5282553.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            2032..2562
                     /locus_tag="PXB44_002115"
     CDS             2032..2562
                     /locus_tag="PXB44_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL5282554.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            2568..2939
                     /locus_tag="PXB44_002116"
     CDS             2568..2939
                     /locus_tag="PXB44_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL5282555.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            2952..3665
                     /locus_tag="PXB44_002117"
     CDS             2952..3665
                     /locus_tag="PXB44_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL5282556.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            3668..4231
                     /locus_tag="PXB44_002118"
     CDS             3668..4231
                     /locus_tag="PXB44_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL5282557.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            4294..4734
                     /locus_tag="PXB44_002119"
     CDS             4294..4734
                     /locus_tag="PXB44_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL5282558.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(4738..>5580)
                     /locus_tag="PXB44_002120"
     CDS             complement(4738..>5580)
                     /locus_tag="PXB44_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5282559.1"
                     /translation="QIKGFSHLYRLRSDLMLKYTEISVTETVEQEPAEFEAGLRLVIL
                     LSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKELVIFLYPKWLI
                     ESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLEKEGNLLALLAS
                     AFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTLQNEFQHYHKTS
                     IFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFGITPSQIKRDKA
                     KGII"
BASE COUNT         1607 a         1184 c         1050 g         1739 t
ORIGIN      
        1 gagttttttt attcaatcac tactttagca atataatcta aatgacgata tttttgggca
       61 taatcaatcc cataacccac cacaaattca tcggggatct caaatcccac ccattttacg
      121 ggcacatcaa cttcgcggcg gcaaggttta tctaataaag tacaaatagc aagtgaattt
      181 ggctcacgta acttgagaat ctcccctacc ttgcttaagg tgaagccagt atcaataata
      241 tcttctacaa tcaatacatc tttaccttga atttcgccat ctaaatcttt tagaattttg
      301 acatcacggc tggattccgt gctgctgcca tagcttgatg cggttagaaa atccacttct
      361 acaggtaact ctagctttct tactaaatct gccataaaca taaacgagcc tcgcaataaa
      421 ccaaccacta caaggttttg gcactgtttt tggtgatagt gttgattgat ttcttttgct
      481 aattcattga ttcggtttaa tacttcttct tttgaatgta gcgtttcgat atgatgtttt
      541 tccattgatt tctccaaata aaattgatta caagcggccg tttttactcg cctgaaacca
      601 tcattttttc taataagatt gaacctgttt ggatatttga gcggtgttca atatcatcgc
      661 ctaccgccac taaattttta tacatatctt gcagttgccc tgcaatggta atttccgcta
      721 cagggtattg aatttcaccg ttttctaccc aaaatcctgc tgcacctcga gagtattccc
      781 ctgttacgct attaatcgca gaacctagca tttccgttac taataatcct gttcccattt
      841 ccttaagtaa cgtatttaaa ccaccttgac gatttggttt aactaaccag ttatgaatac
      901 cacctgcgtg tcctgtactt tttaacccca ttttacgccc tgaatagcta gtcattaaat
      961 acgtttgtag cacgccatca gtaataattt cacggttttg ggtaattaca ccttcactat
     1021 caaacgcaga agaggctaat tgacgcaaca aatgcggacg ctctgaaatc gcaaaccatt
     1081 caggcagaat ctgttcgcct aatttatcta ataagaaact tgatttgcgg tataatgcac
     1141 cgccactaat agctccggcc aaatgcccca ctaaaccggt tgccacatcg ttatagaaaa
     1201 tcacagacat ttcgccggtc ttaattcgtt gcggattcaa tctatctacc gctttaattg
     1261 cagcttgctc ccctacccat ttcggtgctt gtaatttatc aaactcacgg aaaatggtgt
     1321 attcataatc acgctcaagc tgatcttcat aagctgaaat cacgctacaa gagagtgaat
     1381 atcggctcga caggtaactc tgcaacattc cgtgcgtatt gccataaaca cgcacgccac
     1441 tgtgagaatt gaaagttgcc ccgtcactat tcacaatttt ttcgtctgca tttaaggcat
     1501 aatgctccgc ttcaatcgcc aattcgactg cttgatcaac tgaaatatcc gcttggtggt
     1561 acaattccaa atcttctgcc tcaaaggcaa gcaattcttt atcagcaagc ccggtacaag
     1621 catcttctga ggtatatttt gcaatcgcaa gagctgcttc aaccgttttt tgaatcgact
     1681 ctggctgaag atcggaggtg gaagcattac ctttgcgttt gcctaaataa accgaaatac
     1741 cgagagaacc atcgttattg aactcgatgt tttcaacttg ctctaaacgg cttgaaaccg
     1801 aaagcccggc aactttagtc acgcctactt ccgcctctgc cccagcctgt tttgcaaaat
     1861 ttaaggcaaa ttcgaccgct tgtcgtagct cctgttcttg ttgtaacaag gtttgtttat
     1921 ctgtaattga cattttgttc cattgattga ttgcattgat gaatatattc taactcaaat
     1981 ttctgttaga atacggcatt ttttagagtt tatgtaatga aaggataaag aatggctaaa
     2041 aaacggagca aaaacgaaat tgactggaca gatgaggaag aagaaatcat ctgggtcagc
     2101 aaaagtgaga taaaacgaga ttctgaacac ttaaaaaaaa ttggggctga attgatcgaa
     2161 ttgaccccac aaaatttgga aaaaattcca ctagacgatg atttaaaaga ggcaattcgc
     2221 caagctcaag gctttaaaat ggaggctcgc cgccgtcaaa ttcaatttat cggcaaatta
     2281 ttacgcaacc gtgaccagga gccgattcaa gaagccttag ataaagtgaa aaatcgccat
     2341 aatcagcaac aagccctatt acataaattg gaattagtgc gtaatcaact gattgcaatg
     2401 ggcgatgcat cattagataa tttattgacg gaatatcctc aattagaccg ccaacatttg
     2461 cgtaatttaa ttcgtggggc aattaaagag cgtgaagcca ataaaccggc aaaaaattac
     2521 cgtgaaattt atcaatattt aaaaactgaa atcgtggagt gattattatg gagtacctat
     2581 caaccattct taaagctcat atcggcttag cttatattag tttaatttta ttattaaccc
     2641 gtggattttt atcgtcaaag atggtggatt ggcgacaata taaaattttg cgcattgccc
     2701 cacacgcagt tgataccttc ttacttatca ccggtattgc tgctgtcgct attttcttag
     2761 cttatgattt tttcacctta gcacaattca gttggttgct accaaagtta ctcttcttag
     2821 tactttatat tgtatttgcg acaaaagcgt tcaaaaaagg acaaccgttt tcgctcaaat
     2881 tttatctgtt agccgtagtc tcattcttat taatgatgct aactgccact tttcactaaa
     2941 taagggaaac tatgatcggg ccttttgtga atgccggtgc aattatttta gggggcgtgc
     3001 tgggtgcttg tttagggagt cacctgcctg aacgtattcg cactacgttg ccgctggtat
     3061 tcggcttatg ctgtttcggc ttaggtatga tgctgactgc ctcagtaaaa tatatgtctg
     3121 cggttgcctt agcattgatt ttaggcacga tcatcggtga actgatttat ttagaaaaca
     3181 gaatcggtaa agttgccggt ttagctcgtg gtttaattga ccgcctattc ccaccggtgc
     3241 atagtctttc ccaagaagag tttttagaga aatttattgc tctgcttgta ctgttttgtg
     3301 tgagcggtac cggcattttt ggtgcaataa aggaaggaat gacaggcgat ccaagtattc
     3361 tttacattaa atcgattcta gatttcttca ccgcaatgat tttcgctgct tcgttaggct
     3421 atgccattgc ggcgattgca gtgccgttga ttattgtgca agggatttta attctactgg
     3481 caaccgtgat tatgccttta accactgcca atatgatggc agatttctcc gcaaccggcg
     3541 gtttactgct atttgcaacc ggctttcgca tttgtggaat taaaaacttt ccggtcgcaa
     3601 atatgctacc agcactactc cttgcgatgc cgatgtctta tttctgggaa tggttggtac
     3661 attaatgatg aaaaaacgta ttattgtcgg cattagtggg gcaagtggtt ttcaatatgg
     3721 ctataaagtg cttgaactgc tgaaaccgtt ttcagaaatt gaaacccacc ttgtaatcag
     3781 caaaggagct gaactcactc gtactctcga aacggattat acccgagaag ctcttcatga
     3841 tttagctgat caaatacatt cgctaaacaa tgtcggagca agcattgcca gtggttcatt
     3901 taaaaccgaa ggtatggtca tcgccccgtg ttctatgcga acccttgccg ctattgccaa
     3961 cggtttgagc gataatttgc tgacccgtgc ggcagatgtg gtgttgaaag aacggcgtaa
     4021 attggtatta atggtgagag aagctccgct caaccttgct catattgaca atatgcgaaa
     4081 agtcacagaa atgggcggca ttattttccc acccattccg gcgttttacc aaaagccaac
     4141 cagccttgac gagatggtca cccacagcgt aagccatgcc ctttccctgt tggatattga
     4201 cattccaaat accccacatt ggggagaata attcggctta tcttttgctt tttgaaatgc
     4261 tagaatacct gataaaaact atcaggtatt tttatgcaca atccaatccc gacccatatt
     4321 ttagatttca ttcaaactaa ccacgttgtc aatattgcag ctcataccga acaggatttt
     4381 tgggcagcaa attgctttta tgcctttgac caagaaaatg ttcgcttgat tatcttaacc
     4441 gccaagaaaa ccagacacgc tggcttgatg cttgaaaacc cacatattgt cggcacaatt
     4501 tgtgggcaaa ttgagaagat caacgaaatt gaaggtgtcc aattctccgc taccgccgaa
     4561 tgtttagaag ataaacaagc cgaaaaagcc gctttgcaaa tttattacca aaaacacccg
     4621 cttgcacggc ttaagcctag cgatgtttgg gtgttatcat tcaataccat caagcacacc
     4681 ggtaataaac ttatgtttgc gaaaaagacg atttgggaga gaaatataat ttgaagctca
     4741 aataatgcct ttagccttat cccgtttgat ttggcttggt gttatcccaa aagttcgctt
     4801 aaatgccgtt gaaaaattct ccgaattggc atatccggca aattcagctg cttgtgtaat
     4861 actctgccct tgtaatagta atttataggc ttgttctaat ttagaacgcc ttaaatacgc
     4921 aaaaatagag gttttgtggt aatgctgaaa ttcattttgt aatgtggtca cattggtatg
     4981 gaaatatatt gcaatctgct tcaaactcca gccgtcaaat ttaccgctct caagcaattc
     5041 tgttaactgt tgcacccgat ctttttttaa ccggggagcc tgtgtcagct gagaaaaagc
     5101 acttgctaat aaagctaata gattaccttc tttctcaagt aacccaaatg gttgcatttc
     5161 ctgctctgac acaatatcgt gaataagttt taacatactc tgagtcaaaa taatttggtg
     5221 ggcttttaaa tgctgtttaa agtgatgata gcttgccaaa tttaactggc tttctattag
     5281 ccatttaggg tagagaaaga taacaagctc tttttgaagt tgccctttcc taaaaatttt
     5341 acgccccatt tcagctctat ttagcggtaa aaatgcggct tgaggtttta aacttgcatc
     5401 taattcaaat acttccgaat taaaatcaat ttccgatcga ccactcaaca atataaccaa
     5461 tcttagtccg gcttcaaatt ccgcaggttc ttgctctacg gtttcagtaa ctgaaatctc
     5521 tgtatatttc agcataagat ctgaacgtag tcggtataaa tgtgaaaaac cttttatttg
//
LOCUS       DANLUA010000159         5578 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLUA010000159 DANLUA010000000
VERSION     DANLUA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            100..2166
                     /gene="glyS"
                     /locus_tag="PXB44_002121"
     CDS             100..2166
                     /gene="glyS"
                     /locus_tag="PXB44_002121"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL5282560.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVAC
                     SVALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSA
                     QLFGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRA
                     VSHFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEV
                     QPLIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVA
                     DISLLQ"
     gene            complement(2271..3572)
                     /locus_tag="PXB44_002122"
     CDS             complement(2271..3572)
                     /locus_tag="PXB44_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL5282561.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3586..4863)
                     /locus_tag="PXB44_002123"
     CDS             complement(3586..4863)
                     /locus_tag="PXB44_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282562.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4892..5560)
                     /locus_tag="PXB44_002124"
     CDS             complement(4892..5560)
                     /locus_tag="PXB44_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL5282563.1"
                     /translation="MPDKLEYLHDLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAY
                     EKSVIKIINGHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTH
                     IIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFL
                     KAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREF
                     KNNT"
BASE COUNT         1668 a         1114 c         1253 g         1543 t
ORIGIN      
        1 aattaggaaa ttagctagag agctttataa tgaaaatata gcacaattag aaatagaaga
       61 aatcaaagaa catttatata aaaattggta atttaaacaa tgacaacaca aaacttcctt
      121 gccgagatcg gcacagaaga gctgccaccg aaggcactca aaaaattagc gaccgcattt
      181 gcggagaatg tagaaaacga attaaaccaa gcaggtcttt cgtttgagaa agtggaatgg
      241 tttgctgctc ctcgccgttt ggcggtgaag gtgttgagcc ttgcggaaag ccagccgagt
      301 aaagaggttg agaaacgagg gccggcggtg tcggcagcgt ttgatgccga aggcaagccg
      361 actaaggcag cggaaggctg ggcgaagggc tgcgggatta ccgtagagca agctgagcgt
      421 attgccaccg acaaaggcga atggttggtg catcgtgccg tgattgaagg tcagccgact
      481 aaaaatctgt tggtcgatat tatcgccaaa tcgctggcga acttgccgat tccaaaaacg
      541 atgcgttggg gcgataaaac cgagcagttc gtccgccctg tgcataccgt gacactttta
      601 ttcggggcgg agttgatcga aggtgagatc ttaggcgtga aaagcggccg tactattcgt
      661 ggtcaccgtt tcttaggtga acgtgagttc caaattgaga acgccgatca atatccgcaa
      721 ctgttgaaag atcgtggatc tgtggtagca gatttcaatg agcgtaaagc cttaattctt
      781 gcaaaatcac aggaaaaagc aaccgctttg ggtggcgttg ccgatattga agaagatttg
      841 ctggacgaag tgacctcgtt ggtggaatac ccgaacgtac tcaccgccaa gtttgaagag
      901 cgtttccttg ctgtgccggc ggaagcctta gtttacacca tgaaaggcga ccaaaaatac
      961 ttcccgattt attcaaaaga cggcaaattg ttaccgcact ttatttttgt gtcgaacatc
     1021 aatccggaag acccaagcaa aatcatcgag ggtaacgaaa aagtggttcg cccacgtttg
     1081 accgatgcgg agttcttctt caaaaccgac ttaaaacaga agttggaaga ccaactgcca
     1141 cgccttgaaa ccgtgttgtt ccagcaacag cttggcacat tgcgtgataa aaccgctcgc
     1201 attgaagcgt tggcaggtga aattgcgaag caaatcgggg cagatgaaac caaagcgaaa
     1261 cgtgcaggtc tcctctcaaa atgtgacctg atgaccaata tggtgtttga atttactgat
     1321 acccaaggcg tgatgggtat gcactatgcc cgccacgacg gtgaagatga agaagtggcg
     1381 gtggcgttaa acgagcagta tatgccacgt tttgctggcg ataacttgcc aaattcattg
     1441 gtggcttgct cggtggcgtt ggcggacaaa tttgacacgc taacagggat tttcggcatt
     1501 ggtcaagccc cgaaaggcag tgccgacccg ttcgccctac gccgtgcggc acttggctcg
     1561 ttgcggatta ttgtggagaa aaatttaccg cttgatttaa ccgatttagt ggcgaaatct
     1621 gctcaattat ttggcgataa actcaccaac aaagacgtgg ttgaggacgt ggtggacttt
     1681 atgctcggcc gcttccgtgc gtggtatgag agcgaaggca ttgcggtgga cgtgatccaa
     1741 gccgttctcg cccgccgtcc gaccaagcct gccgacttcg atgcccgtgt gcgtgccgtg
     1801 tctcacttcc gcacccttga cagtgccgaa gccttagccg cagcgaataa acgggtgagt
     1861 aacattcttg ccaaagccga tattgcaatt ggcgacattg atgtttcagc ttgcgtagag
     1921 ccagcagaaa aagcattagc cgaggcggtt attgcgttga aagccgaagt tcaaccgctg
     1981 attgcacagg gcgactacac cgctgtgctg gataaattag cgaacctccg ccaaccggtt
     2041 gatgccttct ttgacggagt aatggtaaat gcagaagacc aaaaactgcg tcaaaaccgc
     2101 ctcgccattt taagcacgct acaaggcttg ttcctgcaag tggcggatat ttcgctgttg
     2161 caataacaat aagtttgcaa taattgcaaa attttaatga aaatagaccg cttgcagtaa
     2221 ttgtgatctg caagcggtct tttttgcttg aaattttacc aaaggcggga ttatttaagt
     2281 tttggtttat tcaccataat ctcgatgact tggcggtctg tgtattgcat actacgagag
     2341 gcaatgctgc ataagttatt aattgaacga tcaatatcgt gttcgacaat tccttcgttg
     2401 cctgttactt ggctattatc catagacatc agaatggatt tatagcttga tgttacgctg
     2461 gtggagactt tcatcgcaca actgtttgaa gcaccatcac agataatacc gctaatatcg
     2521 ccgatcatac tactaattgc catactgata ttctcaaatt tccctgtgag cagccacgct
     2581 atgccggcac agcttcccat agccgcggtt gttacggcac agagtgcaga taatttcggt
     2641 aacttgctgt gaatataaat tgccatgagg tgtgataaaa agagtgctct aatgcgttgc
     2701 tcatcagtga catttaaatg gcgggcaacg acaacaatcg gcatagttgc tgtaattcct
     2761 tgattacctg aacccgaatt gctcatagca ggtaaacttg caccgcccat tctagcatca
     2821 gaggcagagg tggtttctat aacaatgcga ttaagcaaat catcactgat tagacctaga
     2881 ccaatctgtt tttgcaatgt ccttccgata tgtagcccat aatcggcacg taatccttct
     2941 tgtgagagtg cggaatttaa ttgtgctgct tgctcaataa agcgaatttt ttccaattcg
     3001 atttgcatag aaaaattaaa tatatctttt gccgaaagtg aagcaaagat ctcagtagta
     3061 ttatcgttat tgacggcaca aggtttgctt aaaatagttt gcccgttttt ttcaatgtaa
     3121 atgacattgg tatggtgttc agcaatacga acacatactc gattcgcttg gtgaaatagt
     3181 gttgcttcag aatatagaat atgttctgtt tcgaaaatag agacagacac tttatcggta
     3241 tttaacatag ctttagcttg cttaacctgt tctggcgtaa tattttttaa tacttctaat
     3301 ccaccatcag gctcccctcc gattgctcca atagctgccg ccataggcaa tccgacggtt
     3361 gcagttcccg gtactgctac tcccatccca tttttcatca agttagggga aacttttgcc
     3421 tcaatacgtt ctggtaaatg acctaaatat tggcgtgcaa tggcggatgc aagggcgaga
     3481 gaaataggtt ctgtacagcc taatgcaggg acaacttcat ggttgactgc atgtaaaatg
     3541 gattgtacta atttttcgca gtgaatattc atagataaaa ccttattact ctaaaaatgt
     3601 gaggaatgga gaaatacata ataaaatgcc ggtaatgata ataatattga gtgctagccc
     3661 tttatatttg aataatgcag gcactttata gactaaatag gccggaatca agcacccaac
     3721 taagccaaaa ataggactac agattgaggt aaagcttaaa accggtgcat ttaatacaat
     3781 cgcactccaa gccagcagaa tggaaaataa cataatccct ttctgcacat aattaggatt
     3841 gattttttca gccgggaatt ttcttaataa taggttcatc acaatgcctt gagtggcttc
     3901 tctaaaacct aaatataccc cgaaaaaggc tgtcataacg gcaaaaatat ttaatgcaac
     3961 acctacatag gttgcccaac taccagggaa aaattgggct gcaattgcta atgcggaaac
     4021 attttgctcg tatgctttga gcgcttcttc tctccccatt gcaagtgtaa aagagacagc
     4081 gtaaaaaaaa acggtaataa ataagatagc aaatgcaata ttcatcgctc gtagggcttt
     4141 atagcgcgca acttcacgag actgttcttt agcacggtaa gaaattacca ttgggctgag
     4201 agtttgcaag aagagtatag aggttagggt gaaaggaagg gtaatgatgg catcttttat
     4261 aagtttgccg aaaggaggca ttgcaccgat attatggagt tgccacattc caatcattgc
     4321 aagccctaag gcggcaacga caaatagctt agttaatacc ataaatgtag atattttaaa
     4381 cagtagcttt tcgcctcgag aagataaggc aactaatata cagattagca ctaagccata
     4441 aaatggatta tcagagagta gtgtattggt aatgccgaag gtaagaagat aggaggcact
     4501 gtcgtttgta attgcggtag aataaacaaa catccaaatc actaacataa taaaataaag
     4561 tacacctaaa aagattcccc agtttttgcc caggtagcca ctaataacgg tggggtaatc
     4621 tttgcattcc ggagattctg ctaaagtatt aatgaacaga cgctgaaaaa ggtacatcgc
     4681 cgggtatccg ataatagaag aaagtagaaa aacccacatg cccataagtc ctacttgtac
     4741 cggtaaaaag acgatgcctg ccccgatagc cataccaata ctcataatga tccagccgaa
     4801 gtcggtagaa tcaaattttg ttgcttgttt aaattcggca ggcgagagtt tttgagtatc
     4861 catagagtct ccttaaatcg taacagtata attaagtgtt atttttgaat tctcttaaat
     4921 gtttataaat tgcgtggctg gttagggaga gtttttctgc cacaataggt actgcctctt
     4981 ttagctcaaa gatgccattt tcaaacaaat agaagataat ggatttctta tagagttttg
     5041 aatgtttggt agtgtgttgc tcaatgtgag taactgcttt tagaaaggtt tcttcaataa
     5101 gttcttttgg gtgagtaata aagttttcac ttttataatg aggcggaata ggatcgtaat
     5161 tcataaaaga gctaacggtt ttatctaatg gtgtcgataa atttaaatta atacaaaata
     5221 atccgatagg tttgccctca tgcccgataa taatatgcgt ggtggactta actaagttac
     5281 cgttatttaa ccgtgaaaag taaaccagct cagtattaga ttgatctttt tggtaacatt
     5341 ttagtaactg taatccttta tctgtaatag gcgaacctat ttttcggtta gtatgatgtc
     5401 cgttaatgat tttaataacg gatttttcat aggcattcaa tgaatgaata accacctcac
     5461 agaattctcc taataaccct gctattgctt cacttaatga gaaataagac agcataattt
     5521 ctttgtctaa atcatgaaga tactctaatt tatctggcat aacttttact ccaatttt
//
LOCUS       DANLUA010000160         5577 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLUA010000160 DANLUA010000000
VERSION     DANLUA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            55..2661
                     /gene="topA"
                     /locus_tag="PXB44_002125"
     CDS             55..2661
                     /gene="topA"
                     /locus_tag="PXB44_002125"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL5282564.1"
                     /translation="MGKSLVIVESPAKAKKINQYLGSNYVVKSSVGHIRDLPKSGSDE
                     KTEKAKPISTKGLTAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSE
                     LKSLAKKADHIYLATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDD
                     PQPLNIDRVNSQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREI
                     KAFQSEEFWTILANTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSN
                     IEAKETSSRAAAPFITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLS
                     REALAMARGYIQDNFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKD
                     AERLYDLIWRRFLACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKN
                     PEDQELPAVELHQKLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIIS
                     TIQERGYVRTENKRFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNW
                     KEELNTFFKHFSEELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLG
                     CTGYALPPKERCKTTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAE
                     RKLHVCGNNPNCDGHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCD
                     NTRKILKNGEVAPPKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAP
                     KVVELAQYRERLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIV
                     DYLDGKWVERKK"
     gene            complement(2711..3649)
                     /gene="yvcK"
                     /locus_tag="PXB44_002126"
     CDS             complement(2711..3649)
                     /gene="yvcK"
                     /locus_tag="PXB44_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL5282565.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3663..4739)
                     /locus_tag="PXB44_002127"
     CDS             complement(3663..4739)
                     /locus_tag="PXB44_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5282566.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4770..5525)
                     /locus_tag="PXB44_002128"
     CDS             complement(4770..5525)
                     /locus_tag="PXB44_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5282567.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
BASE COUNT         1767 a         1047 c         1210 g         1553 t
ORIGIN      
        1 aaagaaataa tttcaaacaa tttaaaataa ttattaaaaa ttaggtaata aattatggga
       61 aaatcactag ttattgtgga gtctccggct aaggcaaaaa aaattaacca atatcttggt
      121 agtaattatg tggtgaaatc gagtgtgggg catattcgtg atttaccgaa aagtggtagt
      181 gatgaaaaaa cggaaaaagc gaaaccgatt tcaaccaaag ggctgaccgc tgccgaaaaa
      241 gctaaaatca aagcagaaaa agagcgtgtt gcattggtta aacgtatggg tatcgacccc
      301 tataatggct ggaaagctaa ctatcagatt ttgccggata aagagaaagt ggtttccgag
      361 ctaaaatcgt tggcaaagaa agcagatcat atctatctgg caaccgactt ggatagagaa
      421 ggggaggcga ttgcgtggca tttgcgtgaa atcattggtg gtgatgacag ccgttttagc
      481 cgagtggtat ttaatgaaat caccaaagat gcgatcaaaa aggcttttga cgatccgcaa
      541 ccgctcaata ttgaccgtgt aaattcccag caaacccgcc gctttttaga ccgtgtagtg
      601 ggctttatgg tgtcgccatt attatggaaa aaagtagctc gtggtttatc ggcagggcgt
      661 gtgcaatcgg ttgccgtaaa attactggtt gagcgtgagc gtgaaatcaa ggcgttccaa
      721 agcgaggagt tttggactat tcttgccaat acgcaaatta atcgtaaaaa attaccgctt
      781 gaattaacta cttataaagg taaaaaattc tcggcaagca atgaggctga agcaaatgtt
      841 gcagtgcgtg ctttggagca gagcgatttt attgtttcta atattgaagc caaagaaacc
      901 agctctcgag ctgctgcacc atttattacc tcatcgttac aacgtgccgc tagcactcgt
      961 ttaggttttg gggttaaaaa gacgatgatg ttagcccaac ggttatatga agcgggttac
     1021 atcacctata tgcgtactga ttcaaccaac cttagccgtg aagcattagc aatggctcga
     1081 ggttatattc aggataattt cggggcgaat tatttgccgg aaaaagcaaa tgtgtatcgc
     1141 agtaaagata atgcccaaga agctcacgaa gccattcgcc cgtcggatgt gaatgtcaaa
     1201 atggcggatt taaaaggaat ggataaagat gccgagcgtt tatacgattt aatttggcgt
     1261 cgtttcttgg cgtgtcaaat gttgcctgct cgttatgatt ctaccgcagt cacagtgtct
     1321 gccggagact atgaactcaa agcaaacggg cgtgtactgc gttttgacgg ctggacgaaa
     1381 gtactgccgg tgcaaggcaa aaatccggaa gatcaggaat taccggcggt tgaactgcat
     1441 caaaaattaa cgcttgatga aattgtgcca agtcagcatt ttactaaacc gcctgcacgt
     1501 tttaatgagg cgtctttggt tgctgagtta gaagagcgaa aaattggccg cccttcaacc
     1561 tatgcttcaa ttatttcgac gattcaagaa cgtggctatg ttcgcaccga aaacaagcgt
     1621 ttttatgtgg aaaaaatcgg tgaagtagtg acggatcgcc tcaacgaatc attcaaagat
     1681 ttaatgaatt atgatttcac tgccaatatg gaagaggtgc tcgaccaaat tgcagagggg
     1741 tctaaaaatt ggaaagaaga gttgaatact ttctttaaac atttctctga agagttgagc
     1801 caagcagaat tagatgaact tgaaggcgga atgaaaccaa acagccttgt gccaaccgat
     1861 attgattgcc cgacttgtgg acgaaaaatg gcaattcgca ccgcttccac aggggtattt
     1921 ttaggttgca ccggctatgc tttaccgcca aaagagcgtt gcaaaacgac aatgaattta
     1981 attccagagg cggaattttt caatgtgtta gattcggatt ctgaaaccaa agccctaatg
     2041 gctcgcaagc gttgcccgaa atgcgattcg gcaatggata gttatgtgat tgatgctgaa
     2101 cgtaaattac acgtttgtgg taataatcca aattgtgatg gtcatattat tgagcaaggt
     2161 tccttcaaaa ttaaaggcta tgacggacct gtagtggaat gcgataaatg cggttcagat
     2221 atgcacttaa aactgggtcg tttcggcaaa tatatgggct gcacaagctg cgataacacc
     2281 cgtaaaattc tgaaaaacgg ggaagttgct ccgccaaaag aagatcctat tgcttttcct
     2341 gaattaaaat gtgaaaaagc cgatgcctat tttgtattaa gagacggagc aagtggggtg
     2401 tttatgtcag ctcataactt cccgaaagtg cgtgaaagcc gtgcaccaaa agtggtagag
     2461 ttagctcaat atcgtgaacg tttgccggag aaattgcagt atcttgccga tgctccgcag
     2521 caagatccgg aaggaaacga ggcaattatc cgttttagtc gtaaagagaa acgccaatat
     2581 gtgacttctg agaaaaacgg caaagccacc aaatggattg ttgattatct agacggtaaa
     2641 tgggttgagc gtaagaaata aagcttaaaa gacaagcggt aaaattttct aaaaatttta
     2701 ccgcttgctt ttagatgttc tgagcgagta gttcatcaat tgctttactt aatttattgc
     2761 gatcgtgtcg gtaataaacg tcattcgcag ataagttttt tcgcaagatt tgaatatgtt
     2821 cgggaagttc agaaccggca tcatcagtcg taattaccgc attaattcta gcctgtccga
     2881 tacagttctc catccattca attcgctgat taagtgatag ttgcccagct gggccatgtt
     2941 cttgtccgat attgtcaata aatactactc gggctttgct ttgagcaata gagtttgcta
     3001 tgcctgccac cagcaaattc ggcataatac tggtaaaaaa gctacctggg cctagcagta
     3061 ttaactcagc actttgaata gctgaaatgg cttctggtgt agcaacaacc atagtatcta
     3121 acgctaaatg ttgtggtgtt tctgctaaag aatcaatttg cacttcacca aatactgatt
     3181 cgcctgaatg caactgtgca attaaatgca ccggtatttc agacattggg ataagttgcg
     3241 atcttacgtg caataattct cgcaccaaat taatcccttc tagcggtcgg atttgcatat
     3301 tttcaagggc ggtgagaatt aaattgccta gattatgtcc agcgagttcg cctgtgccag
     3361 caaaacgata ctcaaacaac cgagaagcta tcgttggttt aacgataatt tgatttaaac
     3421 agttgcgtaa atctccccaa gcaatcaccc cttgttgtga acgtattcta ccggttgagc
     3481 ctccattatc ggtagtggca acaatgccgg taagccgttc ctttaaaaaa gagagcgcgg
     3541 agagcaatcg acctaagccg tggcccccac ctagtgcaac aactgcattg agttggttta
     3601 aatagtgatg tcgggtaggt ttaaaaggaa taatatctgt attttccatc ttgggatttc
     3661 gtttattttt ctaaaataat ggcatctgac tgggctgaag gcaatgcttg taatacagct
     3721 ttaaccaaag tcgctaaagg aatcgcaaaa aacactcccc aaaagcccca taaaccacca
     3781 aagatgagta cggcaataat aattgtcaga gggtgtagat ttactgcttc agaaaataaa
     3841 aagggtacga gtaaatttcc gtctagcaac tgactaataa taaatgcgag cattaaatag
     3901 tagaaatccg gcgataaccc gaactggaat agagcaatta acattaccgg aatgctgact
     3961 aaaaccgcac cgatgtaggg aactaacact gaaatcccta ccgctacaga aagtagtaac
     4021 ggatagcgta aatcaaaaaa gaggaaaata atataagtga caaccccaac aattagaatt
     4081 tctaaaaatt tacctcgaat atagttggca atttgttgtt gcatttcaaa ccatactcgt
     4141 gttgcaagtc gacggttttg cggtaatact ttactaaaag aacgcattaa aacgatttta
     4201 tctttcagta agaaaaagac catgagtggg actaagaagg tgtaaatgcc taagccaact
     4261 aagctgatga tagagttaat cgagagtgtt agaagtgatt ctcccatact caaaatatta
     4321 cttttagcgg tattcacaat ggaatctagg gtcgcataat ctactaattc agggtaatgc
     4381 tccggtaggg cttgtaacca agcattgagc agattaaaca tcgaaggtaa atcttgaata
     4441 aaggtaactg cttgattcca aaggctgggt agtagtacta cgcctaaaaa agataataaa
     4501 gcgatgaaac ctcctagtac tagaatgaca ctcaaagttc gaggtaattt gagtttgctc
     4561 gacaaaaaac ggattggcca ttccaataaa taggcaaaca caagtgcaac cagaaggggc
     4621 ataagcagat cgctaaaaaa atagataata ccgaaaccga tcaatagaat actaaacagc
     4681 acaactattt gcgggtcggt aaattttttt cgataccaat cttgcaacat ttgaaacata
     4741 tggattgcct ttaaattatg cgatgaaaat tatttctggc tacagactgc aatgaaccct
     4801 agctttttgt ccgggtcatt aaatagtttg tacattttat taaaggtttc gcgattttct
     4861 ggttttaatg cattgccgat aattttcatg gcgcctaaaa caccttcatc atgaatcaga
     4921 cctttagggg aaagtagaga catttctccg gaaaaggtat caacattacg gaaaccacaa
     4981 cgttggaata atgatttcca gtctgcttta gcaagtggag agacagtgat attgattgca
     5041 tcacgcaact cggctaataa agtatcggag tctttatctt ggataacaat atcgtgcgtt
     5101 aataaaaagc catttggttt taatacacga aaatattcac gtattgcctt ttctttcgct
     5161 tctagaggaa gcattgtcag catggcttca ttaattacaa tatcaaaact ttcgtcgtca
     5221 aatggcagtt ttgttgcgtt ggcgcgctgt acttgaatta aatgctcaac atggttttct
     5281 ttgatatttt tacgtgcttt ttcaagcgca tcttcgtcta aatcaatacc aacaatttgg
     5341 cattcaaatt gttgagctaa ttggattgca gtagtcccca tgttacaggc aacttcgagc
     5401 acttttttat ttttatcaaa atccccgttt gcaattaacc aatcagttgc taatttcccc
     5461 ccggggcgta aacgagtttt gcctaaacga gctaaaaaat gatgaccaac ttcttctttt
     5521 ttcataaaaa cctctggatt tgcaaaatac gttaaatata tgaccgcttg tattgtg
//
LOCUS       DANLUA010000161         5566 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLUA010000161 DANLUA010000000
VERSION     DANLUA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            11..505
                     /locus_tag="PXB44_002129"
     CDS             11..505
                     /locus_tag="PXB44_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5282568.1"
                     /translation="MTEYLTSFFSLFFSEQNQLFTMFLSGFLSATLLPGNSEIIFTTL
                     ASQHLITYGSLSSFSLFNLLLVATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAK
                     SEKYGVLVLLFSWLPIVGDIFCGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVL
                     PHIL"
     gene            complement(559..1428)
                     /gene="htpX"
                     /locus_tag="PXB44_002130"
     CDS             complement(559..1428)
                     /gene="htpX"
                     /locus_tag="PXB44_002130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL5282569.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(1516..2598)
                     /gene="dinB"
                     /locus_tag="PXB44_002131"
     CDS             complement(1516..2598)
                     /gene="dinB"
                     /locus_tag="PXB44_002131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL5282570.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            2721..3632
                     /locus_tag="PXB44_002132"
     CDS             2721..3632
                     /locus_tag="PXB44_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL5282571.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            3738..5504
                     /gene="aspS"
                     /locus_tag="PXB44_002133"
     CDS             3738..5504
                     /gene="aspS"
                     /locus_tag="PXB44_002133"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL5282572.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
BASE COUNT         1557 a         1215 c         1137 g         1657 t
ORIGIN      
        1 ctacaatttt atgacagaat atctcacttc ctttttcagt ttattctttt ccgaacaaaa
       61 tcaactcttt actatgtttc tgagtggatt tctaagcgct accctcttac ccggaaattc
      121 tgaaattatc ttcactacct tagcaagcca acatctcatc acttatggct ctttatcctc
      181 attttcactg ttcaatttac tcttggttgc aaccttaggt aatagtttag gtagcataac
      241 cacttatgcg atagggctat tactccctcg cccacagaat actaccaatc gttattcaca
      301 atgggcatta gccaaaagtg aaaaatatgg cgtgttagtc ttgcttttta gttggttacc
      361 gattgtaggc gatattttct gtggcattgc cggctggcta cggtttagcc tttggcaaag
      421 cattctcttt attgtgattg ggaaattgtt acgttatctg tttttgctgg caactctcta
      481 ttcggtgtta ccgcatattt tatagaaaac aaaagcggct gttttgcaaa aaattttaca
      541 aaaacaaccg cttgtgcttt ataagtttct taacgcttca atacgttttt ctaacggagg
      601 gtggctcata aacaactctt taccacggct accgttaatc ataaaggcag caagagagcc
      661 ttccatttct tgcggttcgt gaacacgttg caagcgttgt aatgccgcaa tcattttctc
      721 tttaccgact aattgagcag agcctgcatc tgcacgaaat tcacgttggc gagagaacca
      781 cattgcaatc attgttgcta acacaccaaa caccatctgt aacacaatat ctactaccca
      841 gaacaccagc gtattggtgc ttgaattgcc attttcgtca cgtgaacttg ccgccattgt
      901 cgaaataata cgagctaaga aaatgacgaa agtatttaac acgccttgta ataaagtcat
      961 tgtaaccata tcgccattcg caatgtgcgc cacttcgtgt gctactaccg cttcagcttc
     1021 gtctttagtc attgaattca gcaagccggt gctgactgca actaaagaat tacttttcgt
     1081 tgctcctgta gcgaatgcat ttacatcagc cgaatggtaa atcgcaatgt ccggcattgg
     1141 gatattcgct tgctgagcct gacgttgtac agtgttgaat aaccattgct ccgcttcgtt
     1201 acgaggctgt gtaatcacct ctgccccaac cgaacgcaat gccattgatt tcgacataaa
     1261 tagcgaaatt aaagacccac tgaaaccgaa gactaacgaa agcaccaaaa taccggcagt
     1321 gctttgccct tgaatgccgg tcactttaaa aataaggttt aatacgatcc ctaaaacaaa
     1381 ggtaatcgct aagttagtta ataaaaatag aatgacacgt tttgccataa tgttttcaat
     1441 ctccaaaaat atagatgcaa tgattgcacg aataagataa tgactttagc cttaatttca
     1501 agtcattatt tacaattatt cccacaaatt taactgttta ttcacttttt tctcgggaaa
     1561 gtgaacactt agaccgatta atcgcacctt tcgttccttt cttcgggtaa aaatttgctg
     1621 tagcaactcg ccaaatcgct catacgcaaa gccatcggtg gttcgttcaa gcgtggtttg
     1681 gctaaaatca tcaaacttga gtttaatgcc taacttttta aactcagtta atggcgtatc
     1741 gccccagttt cgttgcaacc gaaaaaccag tttgtcgaat aaatctgcca caatcttttc
     1801 cgcttgctca agggttgaaa tatcgtgcaa cagcgtattt tccaccgcca gcgattttct
     1861 ttcacgataa ggctctaccg ctcgttcatc aataccgtgg ctaaaatccc aaagccgttg
     1921 cccgaatttg ccaaacgctc ggtaaataac cgctttttct gcttgctgaa tatcaccaca
     1981 ggtttcaaag ccaagctgtt ttaatttttc attggttact ttccccacac ccggaatttt
     2041 tgcaagcggt agatttttca caaaattttg cacatcatca ggcgtaatca cacattgtcc
     2101 gttaggtttg ttttgatcgg aggtaatttt tgctaaaaat ttaagcggag caactcctgc
     2161 cgaggcagtt aaatgcaatt cattgaaaat atcattgcgg attgcttgag caatccaagt
     2221 ggcggagcca aaatagcgtt cgcattcggt tacatctaaa taggcttcat caagcgacaa
     2281 ggcttcaatc acatccgtat agcgatgaaa aatttcgtga atttgagcgg aaaccgattt
     2341 atacaacgcc atattcaccg gcaataaaat taaattcggg catttcttca acgcagttgc
     2401 cgttggcatt gcactgtgta agccgaattt tcgagcctcg taattacagg ttgtcagcac
     2461 acctcgttga ttagcattgc cgcccaccgc caccggctta ccgaccaacg ccgggttttc
     2521 ccgcatttcg accgctgcat aaaaacaatc catatcaata tggataattt ttcgcttatg
     2581 tttagcttga atcgacattt gcaatatttt ccccaaaaac gaccgcttgc aacacaaata
     2641 actgtatata aaaacagttt tattttatgc ccttattgtt agaatttcaa gtttaaaaat
     2701 agcgataaaa aggataaaaa atgctctacg gtttagccat tgtgctgatt ccactttttc
     2761 tcggttactt aataaccctc aaaaaccacc gccatatggc aatagtaaac agagtggtca
     2821 gcctctgcct ttacttgatt ttattagtga tggggatttc tctcggacaa attgataaca
     2881 ttctcaccga actgccacag attggcggta cggcgtttag tttagtggtg attttactcc
     2941 tttcaaacat tatcggcttg gtgatttacg acaaattaca accgatgaaa agagacaaag
     3001 tcaatcaaga tcaacttccc tcccgcctgc atttgttgat tgattccttt aaattaatcg
     3061 gcattacctt attaggcggg atcatcggct atttcaccaa aggcgtggtg gatttccctc
     3121 tgcacgccag cacctatgtg ttagaagtga tgattttcgg ggtggggatt caactgcgaa
     3181 acagcggtat tcccctgcgt gaagtgttct ttaacaagcg ggggatttat acctcgattg
     3241 taatggtggc aagttccctg attggtggcg tgatttcggc attcacctta gatttatccc
     3301 ttgctaaagg cttaaccttt gcctctgcat tcggttggta ttccctttca agcgtattgg
     3361 taaacgatgc ttggggcccg atttacggca gtattgcctt ttttaacgac atctcgcgtg
     3421 aaattttatg cctgttttta gtaccactgt ttatgcgtca atttccgtcc accgcagtcg
     3481 gcattggcgg agcgacttca ttagattgta tgctccctat tattcaaaaa tcgggcggta
     3541 ttcaaattgt gccgctggca atcagcttcg gttttattgt taatttagcc gctccattgt
     3601 tattagccgt ttttattggg ctggaaggat aaatcaccgc ttgtttgctt ttatttatcc
     3661 atcttcgctg aattttccgc tataatcctt aagttttccc cgaaacttgg gatatttatt
     3721 ttcaaaagga taattttatg atgcgttctc actactgcgg tgcattaaac cgctcacacc
     3781 ttggacaaac agttacctta agcggttggg ttcatcgtgt tcgtaacctt ggtcgtttta
     3841 tttttatgca aattcgtgat cgtgaaggca ttgtgcaggt gtttttcgat gagaaagatg
     3901 aagcactttt caagcaagca tcggccttac gttctgaggc gtgcgtgcaa attcaaggtg
     3961 aagtgattgc ccgtgatgaa tcgcaaatca ataaagagat ggcaaccggt gaaattgaag
     4021 tgttagtaaa aaatttagtg gtgtataaca acgctgaagt attgccgtta gacttcaatc
     4081 aaaacaatac cgaagaacag cgtttaaaat accgctattt ggatttacgc cgccctgaaa
     4141 tggcagaaaa gctcaaaacc cgtgctaaaa tcaccagttt tgtccgccgt tttatggacg
     4201 ataacggttt cttagacatt gaaaccccga tgctgaccaa agctactcct gaaggtgcga
     4261 gagattacct cgtgccaagc cgtgtgcata aaggcaaatt ctatgctctg ccacaatcac
     4321 ctcagctatt taaacagttg ctaatgatgt cgggcttcga ccgttattac caaatcgtga
     4381 aatgtttccg tgatgaagat ttgcgtgccg accgccagcc ggaatttacc caaatagacg
     4441 tggaaaccag ctttatgact gccgaagaag tgcgtgcatt aatggaagcg atgatccacg
     4501 gcttatggaa agagacctta ggcgttgatc tcggtaaatt cccaatgatg acgtggaatg
     4561 aagcaatgac ccgtttcggt tcagacaaac cggatttgcg taacccactg gaaatggttg
     4621 atgtggcaga tattttaaaa tcggttgatt tcaaagtatt tagcggtccg gcgaatgatc
     4681 cgaaagggcg tgtagtggca cttcgtgtgc caaacggtgc agctctaacc cgtaaaaata
     4741 ttgatgacta cacccaattt gtcggcattt atggggcaaa aggcttggct tggttaaaag
     4801 tgaatgatat gaacacaggt ttagaaggca ttcaaagccc ggttgcaaaa ttcttaaatg
     4861 aagaagtgct aaaagcctta gttaaacgct taaacttgca aaaagacgac attgtattct
     4921 tcggtgcgga taactggcac gttgcgacta acgccatcgg tgcgttacgc ttaaaagtgg
     4981 ggaacgattt aggcttaacc gataccacag cgtggaaacc gttatgggtg attgatttcc
     5041 caatgtttga acgtgatgat gaaggcaact tatcggcaat gcaccacccg ttcacttcac
     5101 cgaaagaatt atcgccggaa gaattagtgc aagatccgga aaatgcggtg gcaaacgctt
     5161 acgatatggt aattaacggc tacgaagtgg gcggtggctc ggtacgtatt ttcagccagc
     5221 agatgcaaca aaccgtgttc agcattttag gcattaacga agaagagcaa aaagagaaat
     5281 tcggtttctt attagatgcg ttaaaattcg gtactccgcc acacgcaggc ttagccttcg
     5341 gtttagaccg cttaacgatg ttgattacag gaactgataa cattcgtgat gtcatcgctt
     5401 tcccgaaaac caccgctgca gcgtgtttaa tgacagaagc accaagctat gcgaatccga
     5461 aagcgttgga agagttagca atccgtacaa ttccacaaga gtaatcggta ggggcgaaac
     5521 atttttcgcc ctttttattt aacaagcggt cgaaaaagtg aaaaac
//
LOCUS       DANLUA010000162         5505 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLUA010000162 DANLUA010000000
VERSION     DANLUA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            14..532
                     /gene="hutZ"
                     /locus_tag="PXB44_002134"
     CDS             14..532
                     /gene="hutZ"
                     /locus_tag="PXB44_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL5282573.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NVVHLDQGHQTA"
     gene            682..3294
                     /gene="pepN"
                     /locus_tag="PXB44_002135"
     CDS             682..3294
                     /gene="pepN"
                     /locus_tag="PXB44_002135"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL5282574.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(3354..3626)
                     /locus_tag="PXB44_002136"
     CDS             complement(3354..3626)
                     /locus_tag="PXB44_002136"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL5282575.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(3680..5314)
                     /locus_tag="PXB44_002137"
     CDS             complement(3680..5314)
                     /locus_tag="PXB44_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL5282576.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
BASE COUNT         1609 a         1179 c         1097 g         1620 t
ORIGIN      
        1 attgaggtat tttatgacaa ccaaccgtca agaggtctta caaaaccgtt taggtccaga
       61 aattgaagaa ttaaaacaac aaatcaaaac tataatgctt gcaaccatag ataaagatgg
      121 tgtaccgaat gtgagctatg ccccttttgt aattaataac ggcgaatatc aagtcttaat
      181 cactactatt gcccgtcacg ctcgtaattt acaagaggtg ccaaaagtct ctttaatgct
      241 aattgaggat gaaagtaaga gccgtcaaat ttttgcccgt cgtcgcttaa cctttgacgc
      301 aacagtgcga atgattgaac gtactgatga ggaatggaat aatagcattg cagcacttaa
      361 agctcgtcac ggcgatttag ttgatgaact gtcaaatatg caagatttca agctctttag
      421 ctttaagcca acggcaggct tatttgtaaa aggatttggt aaagcctttg atgttggtgt
      481 tgatgactta attaatgtcg ttcatttaga ccaaggccac caaactgcct aattgcgaaa
      541 taaactcacg gctataaata atcctattta tagccgtttt tattgcgaca agcggtcaaa
      601 ttttttggaa aatttaccgc tattttagta caataacgac actattctat gagctaaacg
      661 caactaaaaa aaggaaatcc tatgcaacct aaagcgaaac tcagaaaaga ttatcgagct
      721 cccgatttca ctattactga tatttatctt gacttccaac ttgacccaga tcgcactttt
      781 gtcacctcaa aactaactgt cgtgcgtaaa aatcctgagg cgacaacact tcgtttagac
      841 ggtcatagtt tcgatttcct ttcgcttaaa tataacggcg agccgttttc cgcctttcaa
      901 aaagatgatg aatctttaac cttaaattta gccgatgtgc ctgccgagcg gttcgagctt
      961 gaaattgaaa ccgcattaaa tccggcacaa aacacctcgt tacaaggttt gtataaatcg
     1021 ggcgacgggt tttgcaccca gtgtgaggct gaaggtttcc gccagattac ctatatgctc
     1081 gaccgccctg atgtacttgc aaaatatacg acaaaaatca ccgctagtaa gagcaaatac
     1141 ccgtatctgc tttctaacgg caaccacatt gcccaaggcg acttgcacga tggcagacac
     1201 tgggtggaat ggcaagatcc gttctttaaa ccaagctatt tatttgcctt ggttgcgggg
     1261 gattttgact tactccaaga taaattcatc accaaaagcg gacgtgaagt cacgcttgaa
     1321 atttatgtcg atcgtggaaa tttagaccgt gcaccttggg cgatggagag cttaaaacgt
     1381 tcaatgaaat gggatgaaga gcgtttcggt ttggaatacg atttagatat ttatatgatt
     1441 gttgccgttg atttcttcaa tatgggagca atggaaaaca aagggttaaa tattttcaat
     1501 tccaaatttg tattggcaaa accggaaacc gcaaccgata cggattattt agatattgaa
     1561 gcggtgattg cccacgaata cttccataat tggacgggca accgcattac ctgccgtgat
     1621 tggttccaac taagcttaaa agagggctta accgtcttcc gtgatcagga atttacctcc
     1681 gatttatggt ctcgttcggc aaaacggatt gaagatgtgc gtttactgcg taccgtacaa
     1741 tttgccgaag atgccagccc gatggctcac ccgattcgcc cggaaaaagt gattgaaatg
     1801 aataatttct acaccgtgac tgtgtatgaa aaaggggcgg aaattattcg tatgattcac
     1861 accctgttag gcgaagaaaa attccagcaa ggtatgcaac tttatgtggc aagacacgat
     1921 ggttctgcgg caacttgtga agatttcgtt aaagcaatgg aagatgcttc aggcgttaat
     1981 cttgaacaat tccgccgttg gtatagccaa tccggcacgc ctgagctcac catttccgat
     2041 gaatatgatg aaacccgcaa ggcataccgc ttgcatattt cacaaatgac accggcaacc
     2101 cacgatcaga tggataaact caatctgcat attccgctta aagtagcgtt atatggtgaa
     2161 caggacggta aacgcattcc gttacaatat gaattattga ccgtaagcga tgtgttagat
     2221 gtaaccgcag aacaccaaac ctttgagttt cataatgtaa cacaacgccc ggtgcctgca
     2281 ttactgtgtg atttctctgc accggtacgg ttagattaca actataccac cgagcagctt
     2341 ttaaccttac tcaaacacgc tgaaaatgat tttgtacgct gggatgcagc tcaaatgctc
     2401 tataccaatg aactgcgtga aaatttaagc cgttatcagc aacatcaacc actcagcttc
     2461 tcagaagggt tggtgaatgc cctaacgttc gtgttggata actcagcaag cctgccggaa
     2521 ctaactgcat taaccctcac attgccaaaa gaaaccgagt ttgcagaatc gttcaaaacc
     2581 attgacccac aaggtattgc ggttgtgcgt gaatttatgc agcattccat tgctcacgcc
     2641 ttgcaagata agttattagc ggtatatcac caaaaccaat gcggggaata tcaagtcgtg
     2701 gctgaagata ttgctaaacg agccttgcgt aatgcttgct taagctattt agcctttact
     2761 gatttaggca acgcgttggt gcataagcat tacaataatg ccgataatat gactgatacg
     2821 cttgctgcat tacaagccgc aaccaaagcc cagctaagtt gccgtgatgc attattagcc
     2881 gattttgagc aaaaatggaa acatgatggt ttagtgatgg ataaatggtt tatgctacaa
     2941 gccacccgac cggacgacga tgtgttatcc atcgtgcaaa atttaatgga acacccaagt
     3001 tttaacttta ataacccgaa ccgtttacgt tcattagtag gtgcattctg cggtcaaaac
     3061 ccgaaagcct tccacgccat tgacggttca ggctatcgtt tcttggtaga tacgctgatt
     3121 aaacttaacg aaagtaaccc acaagttgct gctcgactta ttgagccgtt aattaaactc
     3181 tcacgctacg ataaccaacg ccaaaccttg atgcgtcgtg gtctagaacg cctgcgtgag
     3241 ctggataatt tagcacgaga tctgtttgag aaaattgaaa aagcgttaga ttaaaccaag
     3301 caatacaagc ggtgtcgttt gcaaattttt ttacaaatga caccgcttgt tagttaatac
     3361 cttacctcaa accttttaaa cctttctgag ccaagataat cgctcacaat tacccgttcc
     3421 acctttgcag caggtgagcc ttgttgtaac caattttcca gttgagcgat tgtctgttca
     3481 tccgcttcag ctaccacttc taccgagccg tcatcacgat ttctgacata gccttttatg
     3541 cctaatcgat tcgcttcctg caaggtaaaa aaccgaaaag cgacaccttg aacccgtccg
     3601 taaaccgaga ataattttgc gaccattctt cccccataaa aataggctgc ttatttttca
     3661 ataaacagcc cataatgtct tatttcacga tgtagaggtt acgcagtaaa atatcatctt
     3721 gcggatcttt accctcatag cctttcacgt aaggtttcac caaacgtggg tttacatagt
     3781 tataaatcgg aataatcgca taatctttgg ctaactgctc ctctgctttt gcataagcag
     3841 ctgcacgacc agctgcgtct gtcgctttgt aagattctgc taccgcatca tcataagctt
     3901 ggctcttata aaacgcagta ttgttgctgg agttcgataa gaaatagttc acaaaggtgg
     3961 ttgcttggtt atagtctgcc gaccaacctg cacgagaaac atcataattt gcattgcggc
     4021 gggtatctaa gaatgttttc cactcttggt tttctaattt cacatcaaca atgccgtctg
     4081 tgccttgttt ccataatgat gaaactgcaa tcgcaatttt cttgtggttg tcgttggtat
     4141 tataaagaat agtaaatttt aacgggttcg ctttactgta accggcttct tccaataatt
     4201 tcaacgcttt ggcttttcta tctgccattg aatctttgga ataagcaggt tgctggattt
     4261 tttcaccctc gtgaatataa ggcggtgtaa atacataagt tggtgtttga ccttgtccta
     4321 gcactttatc ggtaataatg gtgcgatcta aagctaagtt taacgcctca cgcactttca
     4381 cattatcaaa cggtgctcgt ttatggttaa tttcatagta ataagtagaa agggttcttg
     4441 ccgtaaacac ttcgtttgga atctcttttt tcaaggttgc aaactgctcc ggtggaagag
     4501 catagttggt catatccaaa tcaccggcac ggtaacgtga aacatcagtt gcggcattag
     4561 caatggctaa gaaagtcgct ttattaatca cggtttcttt atcattccaa taatttttat
     4621 tacgctcaaa cacgattttt tcattaataa cgtgatcggc taaggtataa gccccgtttc
     4681 caaccagatt gcctttttgc acccacgcat cgccgtgttt ctcaacggtt gctttgtgta
     4741 caggtaataa agaagagtgt gttgccagct caactgcata cgggacaggt tggcttaact
     4801 gcacctggaa agtatgatca tcaatcgctt taactgctag ttcctccggt tttttcttgc
     4861 cgtcaataat atcttgagca ttttctacat tgagataagt taaatagctt gaataaggtg
     4921 aagccgtaag tggattaacc aaacgctgcc aagcaaatac gaaatcgtga gcagttacgg
     4981 catcaccgtt tgaccattta gcgtcttggc gtaatttgaa cgtccacgtt ttgtaatccg
     5041 gtgtattttc ccaactttct gccacacccg gctgaatatt gccgtcagaa tctttcgtta
     5101 ccaatccttc aaataactga taagcaactt gcgattccgg cacgccctca attttatgtg
     5161 gatcaaaact tgatggctca aaaccattat taatcgtaat ttcttgtttt tctgcaagcg
     5221 ttgttccttc aggcactttg gctgcaaaca catttgccga taagcctaac actaatgagg
     5281 ttgttaataa ggaaagcgag aattttgact gcatatttga tctccattga tgaatattgg
     5341 cttgcaacca gccgatttgt tctaattaat ttttttaagt ctaggcatta ttattttgat
     5401 tgcaaggaat ttttttaaaa aatgtgatag atatcacctt tcaatgcaaa aattgcataa
     5461 aaaaatcccg aagaaaatag ctctataaac tctgataatg gctag
//
LOCUS       DANLUA010000163         5458 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLUA010000163 DANLUA010000000
VERSION     DANLUA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            27..278
                     /locus_tag="PXB44_002138"
     CDS             27..278
                     /locus_tag="PXB44_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282577.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            262..609
                     /locus_tag="PXB44_002139"
     CDS             262..609
                     /locus_tag="PXB44_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5282578.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(650..1810)
                     /gene="trmG/rlmN"
                     /locus_tag="PXB44_002140"
     CDS             complement(650..1810)
                     /gene="trmG/rlmN"
                     /locus_tag="PXB44_002140"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL5282579.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            2070..2966
                     /locus_tag="PXB44_002141"
     CDS             2070..2966
                     /locus_tag="PXB44_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL5282580.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            2979..3722
                     /locus_tag="PXB44_002142"
     CDS             2979..3722
                     /locus_tag="PXB44_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL5282581.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(3779..4588)
                     /locus_tag="PXB44_002143"
     CDS             complement(3779..4588)
                     /locus_tag="PXB44_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5282582.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(4581..5441)
                     /locus_tag="PXB44_002144"
     CDS             complement(4581..5441)
                     /locus_tag="PXB44_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5282583.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
BASE COUNT         1788 a         1165 c         1086 g         1419 t
ORIGIN      
        1 ataaaactta taaaatagag gcaaaaatga aaatagtggt attccagcgt aaagccttaa
       61 agcaattacg aaaaatccca actggtgcag aaattcgccg aaaatgtgat gatttagctt
      121 actttcccta ctgtgccaat attaaggcat taacaaatca tcaatatgat tatcgctttc
      181 gggtcggaaa ttaccgtatt tttttcaata ttgaaggcga aacgatgaat attgtgtcta
      241 tcgaagaggt taaaaaacga gatgaaagaa cttactaata ttcaatattt aaataatgaa
      301 gaaggaaaac ctgcctttgc cgtacttccg atagcaacac ttgaatggct taagcagaaa
      361 gcaaatatta aaaccgcaat agagaccggt attccatctg ctgttgctgg aattgcatta
      421 gataataact actcagctct gcgtgcttgg cgggaatatt taggtttaac tcaagaagag
      481 gtggcacaac gcttggggat ttcgcaatca gcttatagcc aacacgaaaa tgcaaaaaat
      541 ctacgaaaaa cgacccgcat aaaaattgcc tatgcgttgg gtttaaatga agagcaatta
      601 gatttctaat aacaaaaagg caggaacctc tgccttttca ctattcagat taatgggttt
      661 taaccgcaat accttgcccg aatttacgtt tttcaagggt gcgtttggtg cggtcaatca
      721 catcgccagc aagctgtccg caagcggcat caatatcatc acctcgtgtt ttacgcactg
      781 ttaccgtgaa accgtactcc atcaaggttt tttggaaacg atctacccga gagttagagc
      841 ttttaccata aggagcttct gggaacgggt tccacggaat caagttaatt ttacacggcg
      901 tgttttttaa tacttcagca agttggtggg cgtggtttgt gctgtcattg acatgatcta
      961 acatcacata ttcaatcgtg accttgccgt ggttggcgtt agaaacttca cggtatttat
     1021 tcaccgaatc catcaacatt ttgatgttgt actttttatt aatcggcact aattcatcac
     1081 gcagttcatc gttcggagcg tgaagtgaaa ttgccaacgc cacatcaatt tgctcacgca
     1141 ttttgtctaa tgccggcacc acacccgaag tggaaagcgt gacacgacgt ttagataagc
     1201 cgtaagcaaa atcatctaac atgatttcca ttgccggaat cacgttattc atattcagca
     1261 atggttcacc cattcccatc atgaccacat tggtaatcgg gcgaacaccg gtcacgccaa
     1321 aattgccgat aattttagaa gcacgccaca cttgcccgat aatttccgcc acgcttaagt
     1381 tacggttaaa gccttgttga gcggttgagc agaaggtaca agccaaggca cagcctactt
     1441 gtgaagaaac acacagggtt gcacggtcat cttcaggaat atataccgtc tcgatttgct
     1501 gatcgcccac ttgcattgcc catttaatcg taccgtctgc agaacgttgt tctaccgcca
     1561 cttcaggggc tttaatttcg gcaatacgct ttaatttttc acgcaatact ttattgatat
     1621 tgctcatatt atcgaaattt tcttcgccaa aatggtagat ccatttcatt agctggtcgg
     1681 cacggaaagg tttttccccc atttctgcaa acagttctcg catttcttga cgattaagat
     1741 ttaataaatt gattttttct tttggttgtt cagcagaccc acagcctgca ttggtaatca
     1801 gttctgacat aaagttgaaa gcctcgttat tacacggatt acggcaaaag gttagggcaa
     1861 atgcccttat tttgcgattg gattttaaag cgtgcaattt tactggctct tgagggatta
     1921 ggctagtaat ttttaatcaa aagagttgaa ttgcctgagt aaacttatca agaataacct
     1981 tataaaaaca agactattta catcattccc aatgttctat agaatagctt tcgataattc
     2041 tcattctcaa taacaaaagg aggctaaaaa tgaaactatc aacaattaaa aaagcattgg
     2101 tattaacctt tggtttaagt gcagcgatgg caatggcaga gccgtttaaa gtagttacca
     2161 cttttaccgt gattcaagat attgcacaaa atgtcgccgg cgataaagcc actgtggaat
     2221 ccattactaa accgggtgct gaaattcacg attatcaacc aacaccaaaa gacattgtaa
     2281 aagctcaaaa ggcagactta gtgttatgga acggtatgaa ccttgagcgt tggtttgaac
     2341 gtttctttga aaatgtaaaa ggtaaaccgg cggttgtcgt caccgaaggc atcaccccaa
     2401 tgccgattac cgaaggtgaa tataaaaatc taccaaatcc gcacgcttgg atgtccaccg
     2461 ataacgcatt aatttacatt gaaaatattc gtgcggcttt agtaaaatat gacccacaaa
     2521 acgcagacac ctataatgcc aatgcaaaag cctatgctga aaaagtaaaa ggtattgctg
     2581 agccactccg ccaacgttta gcttttattc ctgagcagca acgttggtta gtaaccagtg
     2641 aaggggcatt tagctattta gcgaaagact acaacttaaa agaactttat ttatgggcaa
     2701 tcaatgcgga agagcaaggt tcgccaaaac aagtgaaaaa agtgattgat ggtgtaaaag
     2761 ccaacaaaat ccctgttgtg tttagcgaaa gtaccgtttc cgacaaaccg gcaaaacaag
     2821 tggcgaaaga aaccggtgca ttatacggtg gcgtaattta tgtggattca ctttcagaga
     2881 aagatggtcc ggttccaacc tatttagatt tgcttaaagt gactatcggc accattgtcg
     2941 aagggttcga acgaagcaaa aaataataag atttagcaat gacaactgtt tcagcctcca
     3001 tttccgtcga aaatttatcg gtccgttata ataacggaca tatcgctttg caagatgtct
     3061 cgtttcatct ggaaaacggc acaatttgtg cattaatcgg cgtgaatgga ggtggaaaat
     3121 ccactctgtt taaaagttta atggggctgg ttaagccatt aaccggctct gttaagctga
     3181 gtcagctccc gattgctaaa gccctcaaac aaaatttggt ttcctatgtg ccacaaagtg
     3241 aagaagtgga ctggcaattt ccagtttcgg tttacgatgt ggtaatgatg gggcgatacg
     3301 gctatatgaa ttttctacgc cgcccaagcc aaaccgataa gctcaaagta gagcaagcaa
     3361 tgcaacgcct tgatgtttta catctcaaag acaggcaaat cggcgaactt tccggcgggc
     3421 aaaaaaaacg ggtattttta gcccgtgcct tagctcaaga aagtaaaatc atcttacttg
     3481 atgagccttt caccggtgtg gatgttaaaa ccgaaaatgc gattgttgag ctacttaaac
     3541 agcttaaaaa tgaggggcat ttgattttag tttccaccca caaccttgcc agcgtaccaa
     3601 gcttttgcga tcaggttttg atggtcaatc gcacgctact ggcaaaaggt aaaacggaag
     3661 aaatctttaa taatcaaaat ttagaacggg tgttcggcgg tttattgcat tatcaaaagt
     3721 agtgtttcag caacaaatca caaacaaata gggaggcaaa tgcctcccta tttttgtatc
     3781 aatgaatctt agaatatgca aaactgagga aaaaaatggc cgcttgcacg agaataatcg
     3841 tcggccctgt tgcggcatca atatggtagc taagcaacgt gccgaatacc gatgaaaata
     3901 tcgcaacgcc taaggcaatc agtaacatac tatcaaaccg cttagtcagc aggtaagcag
     3961 tgatccccgg ggtaatcaac attgccacca ctaaaatcac gcccaccact tgcattgcac
     4021 tgataatagt taaagcaagc aaggaaagta aaccataatg caataatcta accggtaagc
     4081 ctgctacttt ggcttgatta acatcaaagc aatagagcaa gaagtctttg cgtttcgcca
     4141 ccatcaccac aaaggtaaag aggcttatga gtaaagtctg aagcaaatcg cttttggtta
     4201 cgcctaatag attaccaaat aaaatatggg ttaaatgctc accggtatta aaggcggtaa
     4261 acagcaagat cccaaaggca aacatacctg aaaacacaat ccccattacc gtgtcttctt
     4321 tcaagcgact attttctttt aaatagccca ccgataaggc acagcctaat cctgaaaaaa
     4381 acgcccctat tgagagtggt aacccggcaa gtgaggaaat caccacaccg ggcagaacag
     4441 cgtgcgaaat tgcatcgccc attaaagacc agcctttcag caccagatag caagataaaa
     4501 ccgcactcac gccagcaact aaaattgcgg taattaacgc aatttgcata aattcaaatt
     4561 gcaacggctc taaaatccaa ttaagcattt tgaacctcca cacggtgcaa tttccgcttt
     4621 tgagtaatca agccatattt aggagcaaag cagaatgcga ataagaaaat taaggtttgt
     4681 aagcacacaa ttaccgcacc ggttgcaccg tctaaaaagt agcttaaata tgccccaatc
     4741 cctgatgtca gcgtgccgat aagcattgcg atcagcacta atctagggaa acggtcggta
     4801 agtaaataag cagttgctcc cggcgtgacg accatcgcaa taaccaaaat tgccccaacc
     4861 gtttgcagag ccactactac acaggcactt aataaggtaa agaaaatcac tttatagcgt
     4921 aatggcgaaa gcccgactga caatgcgtgg tgttcgtcaa aaaagacgag tagcaaatcc
     4981 ttccaaaata ggattaaacc cagtaacgaa atcgcaataa tcagtaccac ctgccacaag
     5041 tcatcatcag caatgcctaa aatatttccc attacaatcg aattaacatc aaccgatgtt
     5101 ggattgaggg aaacaataaa cataccaagc gcaaaaaaag tcgtgaaaat aaagccgata
     5161 accgcatctt ctttaatttt cgtcaggctt tttacccaaa gaatcgataa tgccgccaaa
     5221 atcccggaaa aaaacgcacc aaaggcgtag ggcaatttta gtgcataagc gatggcaaca
     5281 cctggcacta cagcgtgtga taaggcatca ccaatcaaag accagccttt taacatcaaa
     5341 aaggcggaaa gaaaagcaca aactaacccc acgcccatac tgacaaaaat cgctttttgc
     5401 atatagttgt aggtaaaagg ctctaataaa agatcaagca taaaatccta acaagttg
//
LOCUS       DANLUA010000164         5408 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLUA010000164 DANLUA010000000
VERSION     DANLUA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            47..2614
                     /gene="clpB"
                     /locus_tag="PXB44_002145"
     CDS             47..2614
                     /gene="clpB"
                     /locus_tag="PXB44_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL5282584.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2828..3970
                     /locus_tag="PXB44_002146"
     CDS             2828..3970
                     /locus_tag="PXB44_002146"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL5282585.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4027..5400)
                     /locus_tag="PXB44_002147"
     CDS             complement(4027..5400)
                     /locus_tag="PXB44_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL5282586.1"
                     /translation="MEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLALLIFIA
                     VLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALATALHIQQ
                     LDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGILSKLDQK
                     QNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPIMLILFP
                     LMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKINPIISAF
                     LGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLSAVLGQT
                     GASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFISIAQALN
                     MPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIISIFVIAT
                     VGYLFWL"
BASE COUNT         1766 a         1102 c         1168 g         1372 t
ORIGIN      
        1 gtaaataaaa atttacactt tctaaactca ttgtaaggag aaagaaatga attttgaaaa
       61 aatgaccaca aaactgcaag aagccttggc tgaatcacag tcgcttgcgg taggaaaaga
      121 taatccgtac atcgaaccgg cacatttgct ctatgcgttg ttaaaacaag aaggtggctc
      181 gattgcttct ttgtttacca cattaaatgt tgatgtaccg actttaattc gtgaattaca
      241 acaaatttta gaccgcttgc cgaaagttca aggcggtaac acccaagttt cacaacagtt
      301 ggtcagactg ttaaatcaat cagacaaact ggcacaacaa ttcggcgaca gctttatttc
      361 atcagaacta tttgttctag ctgcacttga tgacaatggc gatttgggga aactatttaa
      421 acaatttggc ttaaacaagg aaaaactaac acaagccatc agccaaattc gaggaggaga
      481 cacagtgaat aatcaaaatg cggaagatac ccgccaagca ctcaaaaaat atacgattga
      541 tttaaccgaa agagccaaag ccggtaaatt agacccggtt atcgggcgtg atgaagaaat
      601 tcgccgtgcg gtgcaagtct tacaacgccg taccaaaaac aaccctgtgt taattggtga
      661 accgggtgtg gggaaaacgg cgattgtaga aggcttggca cagcgtattg taaacggcga
      721 agtgccggaa ggcttgaaaa ataagcgtgt actttcgctg gatatgggag cattaattgc
      781 cggtgcgaaa tatcgtggtg aatttgaaga gcgtttaaaa gcggttttaa acgacctggc
      841 gaaagaagaa gggcaagtta tcctctttat tgatgaaatt cacacgatgg ttggagcagg
      901 caaaacagac ggagcaatgg acgccggcaa cttattaaaa ccaagccttg cccgtggtga
      961 attgcactgt gtgggggcta caacccttga tgaatatcgc caatacattg aaaaagacgc
     1021 agcccttgaa cgccgtttcc aaaaagtatt ggtggacgaa ccaacggttg aagatacgat
     1081 tgcgatttta cgtggtttga aagagcgtta tgaaatccat catcacgttc aaattacaga
     1141 ccctgccatc gtggcggcgg caacgctttc gcaccgttat gtgtctgacc gtcaattgcc
     1201 ggataaagcg attgacttga ttgatgaagc cgcttcaagc ctgcgtatgg agattgattc
     1261 taaacctgag ccgttggata aattagaacg ccgcattatc caactcaaat tggaacgcca
     1321 agcgttacaa aaagaggaag atgaagcgag ccgtcagcgt ttagccaaat tagacgaaga
     1381 aatggcagcg aaagagcgtg aatattccga gctggaagaa atctggaaag cggaaaaatc
     1441 cgccttactt ggtacacagc atattaaatc ggagttggaa aatgcccgaa ttgcgatgga
     1501 tcaggctcgc cgtgaaaacg atttcgagaa gatgtcggaa ttgcaatatg gcgtgattcc
     1561 aaacttggaa aaacagttac aagcggtcga aaatggcgaa aaaaatgcag atgctcagct
     1621 cctccgtact aaagtcaccg aagaggaaat tgcggaagtg ctttcaaaag caacgggcat
     1681 tcctgttgca aaaatgatgg aaggcgagaa ggaaaaactc cttcgtatgg aagaagtatt
     1741 acacgctcgg gttatcgggc aaaatgaagc cgttgatgcg gtggcaaatg cgattcgccg
     1801 tagccgtgcc ggactttccg atccgaataa accgattggt tcattcctat tcttaggacc
     1861 gaccggcgtg ggtaaaaccg agttaagcaa aaccttagct aatttcttgt ttgacgatcc
     1921 tgatgctatg gtgcgaattg atatgtctga atttatggaa aaacacagcg tatcccgctt
     1981 agtgggcgca cctccgggct atgtcggcta cgaagaaggc ggctatttga ccgaagcggt
     2041 tcgccgccat ccatattcgg tagtgttact tgatgaagtg gaaaaagctc accctgatgt
     2101 gtttaacatt ttattgcaag tgttagatga cgggcgttta accgacgggc aaggcagaac
     2161 agtcgatttc cgcaatacgg tagtgattat gacctcaaac ttaggctcgc atttaattcg
     2221 agaaaatgcg tataaaggct atgatgtggt taaagaaatg gtgatggaag tcgtcggaca
     2281 gcacttccgc cctgaattta tcaaccgtat tgatgaaacg gtgatgttcc actcacttgg
     2341 tcaagagcat atccgttcga ttgcgaagat tcagctacaa cgcttaatcc aacgcttggc
     2401 agaacgaggc tatgaggtga cagtaaccga tgcagcatta gatcatatcg gcaaggctgg
     2461 tttcgatccg ctgttcggag caagaccact gaaacgtgcg attcaacaag agcttgaaaa
     2521 tccgcttgct caacagatct taagcggtaa attattaccg aataaagccg ttacggtgga
     2581 ttaccaaaac gggcagattg ttgcaacaca ataatgtctc cgatggtgcg agcgtcctcg
     2641 ctcgtacctt acaagcggtg gatttatcgt aaaaatttgc aaaaaattgc tcgaaaataa
     2701 ccgcttgcac agtaaaaatt ttcatattgc ccttcattaa ttttcataag ctaatgaagg
     2761 gctttttatt actctttttg ctatcattaa cggcgtttat aaccaataca attacaagga
     2821 gtaatttatg agccaattat ttccggaagc aacaatccgc actcaagccc cgtatcgtaa
     2881 tgatattgta ggcagctttc tacgtaccga tgcgttaaaa aaagccagag aagatttcaa
     2941 tcagcaaaaa atcacccgtg acgaattgcg tgaagtcaca cgccaagagg tcaaaaaatt
     3001 agttgagttg caaaagcaac acggtgtaaa agcagtctct gacggtgaat ttagccggac
     3061 ttggtggcat ttagatttct tagctgaact tgatggaatg gattggcaag aaaccgaagt
     3121 ctttaacgtg aactttaccg gtcataaacc caaagggcaa acggtaaaaa tcgtgggcga
     3181 tattgatttc tccgaaagcc acccttttgt ggacgcttat cgtcttttaa aagaagctgc
     3241 cggcgattac ccaaccaaat tcacgattcc ggcaccgaca atgttacata ttatctgctg
     3301 tgtgcgtgcg gaagattacc aaccgttacc acaatatcaa gacgaacaaa aactgtacga
     3361 tgatattgct aatgcttata tcaaagcaat gcacaaattc tacgatatgg ggctacgtaa
     3421 cttacaatta gacgacacca gttggggaca attctgtgcc gaagataaac ggaaagagtt
     3481 tgcagccaga ggatttaatt taactgattt agcggaaaaa tatgtcgcat tattaaaccg
     3541 tattgttgat gcaaaaccgg cggatatgtc aattacgatg cacatctgcc gtggtaactt
     3601 ccgttcttct tggttctcgg aaggcggtta tggaccggtt gccgaagtat tattcggtaa
     3661 atgccgtgtt gacggcttct tcttagaata cgataccgaa cgtgcaggca ctttcgagcc
     3721 actacgccac attaaagatc aacaagtggt actaggctta attacctcta aatttggcga
     3781 tttagaagat aaaaatgtga tcattgagcg tattaaagaa gcggctcaaa ttgtgccaat
     3841 caatcagcta tgcttatcac cacaatgcgg ctttgcctcc accgaagaag gcaatatttt
     3901 aaccgaagcc gcacaatggg cgaaactaaa cttaattaaa gaaattgcgg aagaagtttg
     3961 gggtagctaa tcctctataa tacaaatagc cgagtaaata ctcggctatt ttagtttgtt
     4021 ttaacattac aaccagaaga gatacccaac agtcgcaatc acaaagatag agataatatt
     4081 tagccagaaa ccaactttaa tcatctcact ttgtttaatt tgccctgtac caaatacaat
     4141 cgcattcggt ggggtggcaa ccggcaacat aaaggcgcaa gatgctccta gaccaataat
     4201 taaggctaat ccgaccggtg gcatatttaa tgcttgtgct atagagatga aaatcggcac
     4261 taacaaagcc gcactggctg tgttagaggt aacctcagtt aggaaaataa taaatgctgc
     4321 cacaattaaa ccaattaggt aaaaatgccc cccttcaatt agtgagacga ccccatcagc
     4381 cataattttg ctcgcaccag tttgtcctaa tacagcactt aacgtcaaac caccaccaaa
     4441 taggaataat accccccact cggtgttttc ttgaatctgt tgccaatttg cgacacgagt
     4501 aatgcaaata agtgctgcgg caatcaatgc aagaatggtg tcaaagctcg caattttgcc
     4561 attaattccc agaaatgcag aaataatagg gttaatttta tcaccaaaaa tccaaccaag
     4621 cgcaataaag acgaaaatag ccaaggtgat aatacgagaa cgatttaatt caattttctc
     4681 aaatgatcga tcaaaactaa ctgaaaaatt tggtttaaat acgatatata gtgttgcaat
     4741 cattaatggg aataaaatta gcataattgg caaaccatat tttaaccaat cagcaaaagt
     4801 cagattcaat tgagaagcaa caatcgcatt cggtgggctt cccactaaag tacccattcc
     4861 accaatactg gcactataag caattcctaa taacacgaaa agataagtat tatggttttg
     4921 tttctgatca agtttactta aaatccccat tgccaaaggt aacatcattg cagctgtagc
     4981 tgtattactc atccacattg acaaaaacgc tgttaccgca aataaataaa taactgcaat
     5041 cgataattta cctttagcta atgccataat tttattggca atcatacgat ctagctgctg
     5101 aatgtgtaat gctgtcgcta aggcaaagcc tccaaaaaat aagaaaatcg ttggatcagc
     5161 aaaagcaaca agagcttctt ttgatttaac cagccccagt gctacagcca aaatcggaat
     5221 taataatgcc gtaaccgtaa cgtgtaatgc ttctgttagc cataagacgg caatgaaaat
     5281 taacaatgcc aaccccttat tggcttcagg ggtaaaaggc aaggtattta acaaaataat
     5341 aaataaaata gcatctgcaa caaaaatcaa gctattgcgt gtatttttac tcggttcaat
     5401 aagaggag
//
LOCUS       DANLUA010000165         5289 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLUA010000165 DANLUA010000000
VERSION     DANLUA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            8..1126
                     /gene="rnd"
                     /locus_tag="PXB44_002148"
     CDS             8..1126
                     /gene="rnd"
                     /locus_tag="PXB44_002148"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL5282587.1"
                     /translation="MESLIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPI
                     LGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPE
                     PMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADV
                     WYLLPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREEL
                     AILQVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGK
                     KILWLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKR
                     QLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1183..1665
                     /gene="lrp"
                     /locus_tag="PXB44_002149"
     CDS             1183..1665
                     /gene="lrp"
                     /locus_tag="PXB44_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL5282588.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1676..4336
                     /locus_tag="PXB44_002150"
     CDS             1676..4336
                     /locus_tag="PXB44_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL5282589.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4488..>5289
                     /locus_tag="PXB44_002151"
     CDS             4488..>5289
                     /locus_tag="PXB44_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL5282590.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGL"
BASE COUNT         1664 a          955 c         1203 g         1467 t
ORIGIN      
        1 aaagtatatg gagtctttga ttcactacca atgggttgaa acaaatgagc aattagaggc
       61 cgtatgtgaa caggccagaa aaacaaaagt agttgcctta gatacagagt ttatccgtac
      121 acgtacctat tatccgatat tgggtttaat tcaactcttt gatggccaac aggttagcct
      181 tattgatcca aatacgattt ctgacttctc gccttttgtg tcattattag ccgataaaaa
      241 tatcataaaa gtattacatg cctgtagtga ggatttagaa gtttttgagt atcaatttaa
      301 gcaattgcca gagccgatgt tggatactca ggtgatggca gcctttgccg gcattggtat
      361 ttcgatgggg tttgcaaaat tggtttccca ctacttaaat attgaactag acaaaggcgc
      421 ttcccgaacc gattggcttg ccagaccgct aagcgctgaa caattacaat atgcggcagc
      481 tgatgtttgg tatttattgc cagtttatga gaaattggca caagcattaa caaaaagcac
      541 ttggcaaagt gctgtagcag aggagtgtgt cgctttgttg aagaaagcga aacagcctat
      601 caataaggaa aaagcctata aaaatattgc caatgcttgg cgattgaatc gggaagagtt
      661 agccatatta caagtattag cgaaatggcg gattgaagaa gcacaaaagc gcaatcttgc
      721 cttaaatttt gtcgtgaagg aggcaagttt agttcaaatc gcaaaaagct gtcctaaaca
      781 cacttcttcg ctgttagaat ttatgcaccc aaatgaagtc agaattcacg gaaaaaaaat
      841 actatggttg gttgagcaag gaaaagcaat agaatcgaat gattatcctg aactcattca
      901 gcgcttggtg gatgaaaagg gttataaata ttatatgcaa gcgatgttgc aaaaattatt
      961 agaaattcaa ccgcttgata tcgtaccgga gttggttgca agtaaacgtc aattaaatca
     1021 gctatttaaa tggtatattg atggaaaacc atcagaaaaa attcctgaat tattaatcgg
     1081 ttggcgaaaa ccttttggtg aacagctgct atcggtttta tcttaaattt cattcgggtt
     1141 acatagacta tgtacagtat aattgcctaa aaaggagttt atatggaaaa taaaaaacca
     1201 ccaaaagcat tagatgcgat tgatttaaaa atcttaaacg agctacaacg taacgggaaa
     1261 atttctaata ttgaattatc aaagcgagtt gggctttctc caacgccctg cttagaacgg
     1321 gtaaaacggc tagaaaaaaa taacgtaatt atgggctata aagctttact taaccctgaa
     1381 ttacttgatg cccctttact ggtgattgtt gaaattactt tggtgcgtgg taagcctgat
     1441 gtatttgaag aatttaataa agcgattcaa caacttgatg aaattcaaga atgccacctg
     1501 gtatcggggg attttgatta tctgttaaaa acccgtgtgg cggatatggt atcgtaccgt
     1561 aagttattag gcacaacatt gcttcgtttg cccggcgtaa atgacactcg aacctatgtg
     1621 gtgatggaag aagtaaaaca aaccaactat ttgttgttaa agtaaggaga attgggtgat
     1681 tgaacaactt aaaccacacc taaaaggcaa agagaacttg atcaaactta tgcttttgtt
     1741 gaccggcttt ttgggcgttt atttgctaat tgcttgggca agttatagtc cattagataa
     1801 tgcttggtct gttgcaagca gcattacaca aacaaccttg aataaaacag gtcactttgg
     1861 tgcttggagt attgatatgc tctatgcaat gtttggtaag gttgcagttt tagtacctat
     1921 tgcattaatt attttctcaa tttatagctt gggaatgggt ttaagtgtcg atatgaaatg
     1981 gaaaactgtc ttcattcgcc tagccagttt cttattattg atggtcggtc ttgccgggtt
     2041 atttagcgtg cttttttcaa actcagctta ttatctgtcg ggtggctttg tcggtggagt
     2101 ttgggagaca atattatctg atactatcgg acaatttggt gccttattga ttgcaatgct
     2161 ttttactata gctggcttat atttttgctc tgttcaatca ttattgccaa tattaacgca
     2221 attttatgag tggataatgg caaaatcaga agagagcaaa acagctgaat ctcttcagca
     2281 tttgcaaaat actgagcaaa aacaaccgct tgtaactgaa aatgaagcgg tggaagttgt
     2341 agatgaccat aaaccgcaat ttaccgatat ctccgccttt aaacgcccaa acattagtgg
     2401 tttaagacac tcggaaaatg tagagataga taaccgagaa atgtatgaaa ttgaaagaga
     2461 tcttgagcta ccgactatta atattttaat acagcctgat atggttaacc aagttgcgga
     2521 acaagctaca tcgggctatg agttaccaaa cgaagaatcc aatgtattgc aaatgccgac
     2581 cattcgctta aataccgcta tcgaaaacga ggtggataca atcgaaaaca gttcatcatt
     2641 ggaagacctg aaaatagcag aatctatgtt acctcaaatt cagcttgata atgatattga
     2701 agatactgaa gaagctattg acgatgtgcg tgaaattaca ccggaattta tggtaacgcc
     2761 gcccaaggcg atgaaagatg tgtctgagca gccagcctat ccaaaaggct atggtgatac
     2821 gttaattcac ccgttgttac aacgaaaagt gataaccgaa aaaccgacaa caccattgcc
     2881 aacgctagat ttgttggata aagcaccgat tcaaagccag caaattaccg aacaggaaat
     2941 acgagaaacc tctgtgcggc tagaagcaga attagcaaac tttggcgtaa aagctaccgt
     3001 tgaagatgta ttggtcggtc cggtagtgac ccgttatgaa atccaaccgg ctgccggggt
     3061 aaaagcctcc aaaattacca atttagcgag cgatatagct cgtggcttaa tgtttaaagc
     3121 gattaggatt actgaagtta tcccaaacaa accttatatg ggtattgaaa ctccgaataa
     3181 acaccgtgaa acagtgtggt tgcgtgatgt gctagacagc gatgaattcc gcaataccac
     3241 tgcaacattg ccaatggcac taggtaaaga catcagtggt gagcctgtgg tggtcgatat
     3301 ggcgaaaatg ccacacttac tggtggcagg gcagaccggt gggggtaaat cggttggggt
     3361 gaatacgatg attttaagtc tattgttcaa acttacacca gaacaagtcc gctttattat
     3421 gattgaccct aaagtggtgg agctttccat ttataacgat attcctcact tattaacgcc
     3481 ggtagtaacc gatatgaaaa aagccgccaa cgcactacgc tgggcggtgg aggaaatgga
     3541 acgccgttat ttgctggtaa gccatttaca ggtgcgtaat attgaaggct ataatgcgaa
     3601 aatcgaccaa gcggcagcga tgaatttgcc gattccagat ccaacgtggc gaccgggcga
     3661 ttcaatggat agccttccgc ctccgctaca aaaattaagc tatattgtgc tgattgtcga
     3721 tgagtttgcg gatttaatga tgtctgccgg taaagaagtg gaagagtaca ttatgcgaat
     3781 tgctcaaaaa gcccgagcgg tgggaattca cttaattctt gctacccaac gtccgtcaac
     3841 cgatgtgatt accggcgtga ttaaagccaa tattccaagc cggattgcgt ttacagtagc
     3901 gagccaaatt gactcaagaa ctattttaga ttcaggtgga gcagaggcat tattagggcg
     3961 aggcgatatg ctctattcag gagctggcag cccggatatt attcggatac acggtgcgtt
     4021 tatgaaagac gaagaagtgc aacgcgtggc ggataattgg cgggcaagag gaaaaccaaa
     4081 ctacttggaa agcattgtgg aatcccgctc ggaagaggct gatggcaaaa acgatggcgg
     4141 aacgggcgat ttagatccgc tatttgacga ggttgttgaa tatatcacgg aaaccggttc
     4201 ggtctctatc agtaatattc aacgccgctt ctcgcttggt tttaaccgtg cggctagaat
     4261 tgtggatcaa atggaagctc aaggcattgt ttctgagcca cttaaaggtg gcaaacgaga
     4321 agtgctggcg aggtaaaaca catacaagcg gttagatttc tgtgaaattt tgcaaatttt
     4381 gacaaaaatc taaccgattg tatttatcta atgctaaata agcatagtat aaggctagtc
     4441 tttgggctaa aattaacgca atttttacat cttttaaggt tgaatatatg cgattagatg
     4501 atattaaaga aagtaaaaat gttgaagaca gaagattcaa caatgtgggt agaactaagg
     4561 ttggtagagg gaaaggtgga atattaacct ttattatcgt gcttgttgga gcttattatg
     4621 gtgttgactt aacaggttta atgggcggtg gcgtagaata tgaggagcag acatcagcat
     4681 taaatgaaag tgaagaaaaa cagctaagaa ccttatccgc taaagtatta acgaccacag
     4741 aaaatatttg gacttcctac ttccagcaaa atggtctaac ttataaagag ccgtctttgg
     4801 tgctttaccg tggggcaatt caaactgcct gtggcaccgg tcaatctgca atgggtcctt
     4861 tctattgccc tgtcgatcaa aaggtttatt tagatttatc attctatgat gatatgcgta
     4921 aaaaattaag agcatcgggc gattttgcct tctcttacgt gattgcacac gaggttggcc
     4981 accacgtcca aaatttattg ggcataacaa gcaaaaccca acgggctcaa atgaaagcct
     5041 catctaaagc cgaagcaaac caaatttcag tcaatgtaga attgcaagct gactgttttg
     5101 ccggcgtttg gggacaccaa ctcgcaaaag ccaaccgatt agaggaaggc gatgtggaag
     5161 aagcctttaa tgctgcccaa gcagttggag atgaccgatt acagcaacaa agtcgtggct
     5221 atgtcgtgcc ggatagcttc acccacggct catctgcaca gcgtttggct tggtttagaa
     5281 aaggtttaa
//
LOCUS       DANLUA010000166         5286 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLUA010000166 DANLUA010000000
VERSION     DANLUA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            918..1055
                     /locus_tag="PXB44_002152"
     CDS             918..1055
                     /locus_tag="PXB44_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282591.1"
                     /translation="MKPLRIGARVIYPLQDLDKQIAYLEANLLENIKVHFIVESIEIK
                     G"
     gene            1190..2107
                     /locus_tag="PXB44_002153"
     CDS             1190..2107
                     /locus_tag="PXB44_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class A beta-lactamase
                     ROB-1"
                     /protein_id="HDL5282592.1"
                     /translation="MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQA
                     TFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKALLAGAVLQSLPE
                     KDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVE
                     QYQRILRQLGDNVTHTNRLEPDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQK
                     TILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKPIVMAIM
                     STQFTEEAKFNNKLVEDAAKQVFHTLQLN"
     gene            2118..3035
                     /locus_tag="PXB44_002154"
     CDS             2118..3035
                     /locus_tag="PXB44_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5282593.1"
                     /translation="MKKKLLWILILGLIIISCKQRKTEMKEKIIKTNGIELCTESFGN
                     KKNPAILLVAGATVSMLYWDTEFCQQLSEKGFFVIRYDNRDVGKSTNYEPGSTPYDIV
                     DLTNDAISILDGYKIDKAHFVGISLGGLISQIASIKFADRVNSLTLMSSGPWGDSDPT
                     IPEMDTSILDFHSKAGTVNWTNEDSVVNYLIQGAELMSGKKQFDKQRSEKLIRAEFNR
                     ANNYISMFNHAALQGGGEEYWNRLNEIKQPTLIIHGTDDKIWHYKNAGFLLEKIKGSN
                     LITLEGTGHELHVDDWKSIIDGIEKHIND"
     gene            complement(3360..4766)
                     /locus_tag="PXB44_002155"
     CDS             complement(3360..4766)
                     /locus_tag="PXB44_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL5282594.1"
                     /translation="MIVKFFKKHGKGKASSCKACVDYLLNKPDDTAQILQGDPRLSQS
                     IADSLDFNNTYTAGCLSFEESDLPEIQKREIMARFEKAMFAGLEPEQYNIAWVQHTDK
                     GRLELNFVIPNVEMTSGKRLQPYYDRADRPLAENFKQVINHEYSLSDPNNPIKQKNLI
                     DRKDLPTDKKQALQAITDGLTALANAGQINDRQDVINALERAGFEIARITPKNLSIKT
                     DGQNLRLKGAFYEQDFRFSTDLSADITERAREYKRDSAERYQTARAKLDTAVTARREQ
                     FSRKYPNRAGEIDKKYRENVSLADPNRLDDISLDSHNRRRFDVAGEERLSRDDRMAKF
                     SRDSQRAEWGDQAINLQNGERDKALCSSGQGFYQLNVGREPESNRNPRNQETGEKING
                     DRTRTTLRENLENLARTARNRASEIIERIRELASRKRLDHSTVQRNQRAINDITEFNK
                     QLETVISQRKRQSRGMSR"
     gene            complement(4763..5068)
                     /locus_tag="PXB44_002156"
     CDS             complement(4763..5068)
                     /locus_tag="PXB44_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL5282595.1"
                     /translation="MKREKEIKIRLTENEYQALLERKTKARLAEWVREVALEQQPKRQ
                     PKVIDPALLFELNRIGVNLNQIARQCNSQKPSIDLVSVLATLREIEKNLKKLRELSL"
BASE COUNT         1490 a         1075 c         1081 g         1640 t
ORIGIN      
        1 tttttagtgt tggaaagctg ttggaaagct cgtgggtaac tcgcagagtt atccatcagc
       61 ttttcaatgg cttttcaata cgacccgcag gggaaaaacc gttaaaccgt ccacattaac
      121 cgctgaccca aaaagaacgc tttttactaa aagatttcta ttcggttctc aaatgttgcg
      181 tgaatggggc aacaataaaa cgttgtggcg tttatcaggg tgggaattgc agacttaaac
      241 aatcaattcc gctgtatcct taaatctcgc ttatcaggtg tgcttgtatt tcttggggct
      301 ttcgcctttc taaccgtagc acgaaacgga gacagagctt aattttcgta ttaagaacgt
      361 tatcacttct acgcctgttt attcttccga agtccgagct gaagaccgtc taatttaagg
      421 tttacgtttc ttgtttacgt cgttgaatgg ttttttggta gtagggacgc ttcgcaatct
      481 cttgcattac tagctaccct ttgctatgat tagattgttc acgtctggat catagcaaag
      541 aacgtactga tacagattat aggaaaaggg atcgaatgtc aaaagttcga tccctttttc
      601 ttttctaaag cattggtatt aaaggcttta aggtgtattt tattaaaatc tgtacacctt
      661 tgtgaaaatc catacacctt tcttaccgtg taattttatg aaaattcgtg cacctttctg
      721 ctaacgccct gcggttgtta gactaaaaaa gccattgaaa gcaagtttca acggcttttt
      781 cagtcccgaa taaaacaaaa ccgccttgtt aggcggttat gtgttaaaat cactttcgca
      841 ctatggcggc ggctcgcact accgttcata gtgtgagcct agtcgcttag ccttatagtg
      901 ctaagggggt gatttatgtg aaacctctac gaattggggc aagggttatt tatccgcttc
      961 aagacttaga caagcaaatt gcatacctag aggcgaactt acttgagaat atcaaagtgc
     1021 atttcattgt agaaagcatt gagattaaag gctaaattgc tagacttttg cgcttaaatt
     1081 cgccaaaatc tgtttttttg ttctacaaac aaattatccg aatttccgcg caatgtacgt
     1141 ttcaggctgc ctgaaaagaa atttatttag taaaatcaaa ggataattta tgttaaataa
     1201 gttaaaaatc ggcacattat tattgctgac attaacggct tgttcgccca attctgttca
     1261 ttcggtaacg tctaatccgc agcctgctag tgcgcctgtg caacaatcag ccacacaagc
     1321 cacctttcaa cagactttgg cgaatttgga acagcagtat caagcccgaa ttggcgttta
     1381 tgtatgggat acagaaacgg gacattcttt gtcttatcgt gcagatgaac gctttgctta
     1441 tgcgtccact ttcaaggcgt tgttggctgg ggcggtgttg caatcgctgc ctgaaaaaga
     1501 tttaaatcgt accatttcat atagccaaaa agatttggtt agttattctc ccgaaaccca
     1561 aaaatacgtt ggcaaaggca tgacgattgc ccaattatgt gaagcagccg tgcggtttag
     1621 cgacaacagc gcgaccaatt tgctgctcaa agaattgggt ggcgtggaac aatatcaacg
     1681 tattttgcga caattaggcg ataacgtaac ccataccaat cggctagaac ccgatttaaa
     1741 tcaagccaaa cccaacgata ttcgtgatac gagtacaccc aaacaaatgg cgatgaattt
     1801 aaatgcgtat ttattgggca acacattaac cgaatcgcaa aaaacgattt tgtggaattg
     1861 gttggacaat aacgcaacag gcaatccatt gattcgcgct gctacgccaa catcgtggaa
     1921 agtgtacgat aaaagcgggg cgggtaaata tggtgtacgc aatgatattg cggtggttcg
     1981 cataccaaat cgcaaaccga ttgtgatggc aatcatgagt acgcaattta ccgaagaagc
     2041 caaattcaac aataaattag tagaagatgc agcaaagcaa gtatttcata ctttacagct
     2101 caactaacaa attcagcatg aaaaaaaaac tactttggat attaatttta ggactgataa
     2161 taatcagttg caaacaaagg aaaacagaaa tgaaagagaa aataattaaa acaaacggca
     2221 ttgaactctg tacggaaagt tttggaaata agaaaaatcc agcaatcctt ttggtagcag
     2281 gtgcaaccgt atcaatgctg tattgggaca ctgaattttg ccaacaatta tctgaaaaag
     2341 gattttttgt tattcgttac gacaacagag atgtaggaaa atccactaat tatgaaccag
     2401 gttctactcc atacgatatt gttgacttaa ctaatgacgc tatttcaata ttggatggct
     2461 acaagattga caaagcacat tttgtgggga tttctttggg cggactaatt tctcaaatag
     2521 catcaataaa gtttgccgac agagttaact ccttaactct tatgtcatca ggcccttggg
     2581 gagactcaga cccaactata cctgaaatgg acacgagtat tttagatttc catagtaaag
     2641 caggtacagt caattggaca aatgaagaca gtgtggtaaa ctatttaatt cagggtgcag
     2701 aattaatgag tggcaagaaa caatttgaca aacaaagaag tgaaaaactg ataagagctg
     2761 agttcaatag agccaacaat tatataagta tgttcaatca cgctgcattg caaggtggtg
     2821 gtgaagaata ttggaacaga ttaaacgaaa tcaaacaacc caccttaatt atccacggaa
     2881 cagacgacaa aatttggcat tataagaatg caggtttttt actagaaaaa ataaaaggtt
     2941 caaatctaat cacccttgaa ggtacaggac acgaattaca cgttgatgat tggaaatcaa
     3001 taattgatgg aatagaaaaa cacataaatg actgataaac aaaaacaacg acccgctaac
     3061 aatgtatata aaaaataggc gaaacagtag taaattcaag gcttttggct cgtattatac
     3121 tttgtgctta accgaaaatt tggcacttcg aaatcgccta cttttcgctg aatttacaat
     3181 ccaagtgcaa caaaaaaaga agtactcatc aactagaata taattttgtt tccacacaaa
     3241 aaccacttcc aaatgatgag tacttcctac cgacatctta caataagccg agtgtttgag
     3301 aaataccatt tattaaactg tctttttcca taagtgttgc cataattctc ccccttgttt
     3361 tatctgctca tacccctgct ttgtcttttg cgttgagata ttaccgtttc aagctgttta
     3421 ttaaactctg taatgtcgtt aattgctcgc tgatttcgtt gtactgttga atgatcaagg
     3481 cgttttcggc tcgcaagctc tctaattctt tcgataattt cactcgctct gtttcttgct
     3541 gttctagcaa ggttttcaag gttttctcgt aaagtagttc tagttcggtc gccatttatt
     3601 ttttcccctg tttcttggtt tcttggattt cgattacttt caggttctcg ccccacgttg
     3661 agttgataaa atccttgtcc actgctacac aaggctttat ccctttctcc gttttgcaag
     3721 ttgatagctt gatcccccca ctctgctctt tggctatctc ggctgaattt tgccattcgg
     3781 tcatctcttg ataggcgttc ttcgcctgcc acgtcaaaac gcctgcggtt atggctatca
     3841 aggctaatat cgtcaaggcg attagggtcg gcaagcgata cattctcacg gtatttttta
     3901 tcaatttcgc ctgctcgatt tggatatttt cggctaaatt gttccctgcg tgctgtaact
     3961 gctgtatcaa gttttgctcg tgccgtttga tagcgttctg cactatctcg cttgtactct
     4021 ctagctcttt ctgtgatgtc tgctgaaagg tctgtgctaa atctaaaatc ttgctcatag
     4081 aaagcccctt ttaatcttaa attctgtccg tcagtcttga ttgataggtt ttttggcgta
     4141 atgcgtgcaa tttcaaaacc tgcacgttct agggcattta taacgtcctg tcggtcgttt
     4201 atctgccctg cgttcgctaa agctgttaaa ccgtccgtaa tcgcctgtaa agcctgtttt
     4261 ttatcggttg gtaggtcttt gcggtcaatc aggttttttt gctttatagg gttgtttggg
     4321 tcgcttaggc tgtattcgtg gttgattacc tgcttgaagt tttcagcaag tgggcggtct
     4381 gccctgtcgt agtagggttg taggcgtttt ccgcttgtca tctctacgtt tgggataacg
     4441 aaattcagct caagcctgcc tttgtcggtg tgttgtaccc acgcaatgtt atattgttca
     4501 ggctcaagcc ctgcaaacat tgccttttca aagcgtgcca taatctcacg cttttgtatt
     4561 tcgggtaggt cgctttcttc aaaagacaag caacctgcgg tgtaagtgtt attaaaatca
     4621 agactatcag caatactttg tgatagtcgg ggatcgccct gcaggatttg ggcggtgtcg
     4681 tcaggcttat ttagtaaata gtccacacaa gccttgcaac tactagcttt tcccttaccg
     4741 tgtttcttaa aaaatttaac gatcataggc tcaattctcg caattttttg agattttttt
     4801 caatttctcg caaggtcgcc aacacgctaa caaggtcaat gctcggcttt tgactgttgc
     4861 attgtcgggc gatttggttc aggtttacgc ctatgcggtt cagctcgaac agtaacgcag
     4921 ggtcgattac tttcggctgt cgcttaggtt gctgttctag ggcaacttcc cgaacccact
     4981 ccgcaagcct tgctttcgtt tttctctcta acaaggcttg atactcgttt tcggtgagcc
     5041 tgatttttat ctctttctca cgtttcataa tggtgtccgt tcttctattt tgaccgctga
     5101 aagcggtcgg gggtcaaggg ggcaaagccc ttttgcaagg tcaaaaagga aactcgaaga
     5161 gtaccctttt tgtgtccttg ctattaaaaa agacaatagt acaccaaaag ccccgagagt
     5221 gatatataaa aatcagcgga gccgataggc gacttcgttt tattaggcga acatcgtgag
     5281 cctttg
//
LOCUS       DANLUA010000167         5227 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLUA010000167 DANLUA010000000
VERSION     DANLUA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            33..704
                     /locus_tag="PXB44_002157"
     CDS             33..704
                     /locus_tag="PXB44_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL5282596.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
     gene            959..1288
                     /gene="trxA"
                     /locus_tag="PXB44_002158"
     CDS             959..1288
                     /gene="trxA"
                     /locus_tag="PXB44_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL5282597.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            1459..2682
                     /gene="hflK"
                     /locus_tag="PXB44_002159"
     CDS             1459..2682
                     /gene="hflK"
                     /locus_tag="PXB44_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL5282598.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            2685..3572
                     /gene="hflC"
                     /locus_tag="PXB44_002160"
     CDS             2685..3572
                     /gene="hflC"
                     /locus_tag="PXB44_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL5282599.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            3746..5044
                     /gene="purA"
                     /locus_tag="PXB44_002161"
     CDS             3746..5044
                     /gene="purA"
                     /locus_tag="PXB44_002161"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL5282600.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
BASE COUNT         1623 a          913 c         1197 g         1494 t
ORIGIN      
        1 taggtattct aattattttt gaggatatga aaatgaaaaa atcaacttta gcaattattt
       61 taggtggggc attattaact ggaactgcgg tggcacatca agcaggtgat gtgatattcc
      121 gtgccggtgc ggtacatgtt aaggcaaatt caagttcaga tgcaaaatca aaagcagcga
      181 caggtattga tgtagattta gaagtgaaaa acaatactca gttaggctta actgcaactt
      241 atatgttagc cgataatgtg ggtattgagt tattaggtgc aacccctttc tctcataaaa
      301 ttaatgccaa agccaaagtt ttagaatcag gtgcagaagt agatttggat agagttgtta
      361 tcttaaaaca attaccaccg agcctttatg cccaatacta tttctttgag ctaactgcaa
      421 aagtaaaacc ttacgttggt gcaggtttaa attacactcg cttctaccac gctaaatcag
      481 ttagccctgc tgttactgac ttaagtgtaa aaaaacattc tttcggaccg gtagcaaacc
      541 taggtgttga tgtaaaatta accgataaca tctacttcaa cgcggcagct tggtacacac
      601 gcattaaaac aaccgcaaaa tttaaagcag caggtgcaga tcacgaagta aaagtaaaac
      661 ttgatccggt tgtcttattt gccggtttcg gggtgaagtt ctaattattt gttgggaacg
      721 agttgggcta atcttagtga ttagtccttt tttgttaaag ttgtttgaat atagcccttt
      781 ccacttttaa ttaaataatt gaagagataa taaaatctca ctatgcaaat atttattggt
      841 gtaattggta aatcttgctt gaaattttat agattaatct ctatcttata caaaactgtt
      901 cgccttaact tgccagttag ggcgtttctt tttttattta atatttaagg ataaacttat
      961 gagcaatgtc attcatacaa cagatgctac atttgaacaa gatgttttaa aatcagatgt
     1021 accggtatta ttagacttct gggcgccttg gtgtggtcct tgccgtatga tcggccccgt
     1081 acttgacgat ttatctgttg atgcacaatt tgatggtaaa gtaaaaattg tcaaaatgaa
     1141 cgtagatgaa aatccatctg taccagcaca atttggcgta cgcagcattc catttttact
     1201 attattcaaa aatggtgaag tgattgccca gcaagttggt gcaattccgc acgcccaaat
     1261 ggctgaattt atcaaacaag cattataatt ttaagagcaa tactagggcg aatttatcgc
     1321 cctatttttt tgcaaataaa tagtgaaaaa agaccgcttg tttcagataa acctagtcaa
     1381 aagcttacta aataggtaca atcactaact tgcattctaa gctttaaagt gcgattcgat
     1441 tttattttgg agatatttat gtcgtggaat gaatcaggca atcagcaaga tccttggggt
     1501 aaaccggggc agaaaaaagc tgagccaaag cctgagcaac aagaacaggg ttcacaacaa
     1561 gagccgacca ataatcaaag aaatgaacaa cagccccctg atttagaaga agtatttagc
     1621 agtttattaa aaaaaatggg gggtggaaaa gggcctaata atagttcaaa tcaacctagt
     1681 gcaagcttag ggaaatttct acctgtggtt ttaggtcttg cagcagtagt ttgggttggc
     1741 tcaggttttt acactgtgca agaggctgaa cgtggtgtgg ttactcgttt aggtaagctg
     1801 aatgatatcg tgttgccagg gttgaattgg aaaccgactt ttattgattc ggttacaaga
     1861 gtaaacgtag aacgtgtttc agagctgaac accagtggct caatgctgac tcaagatgaa
     1921 aatatggtgc aggtagaaat gacggttcag tatcgtgtgg aagacccggc taaatatctg
     1981 tttagtgtga gcaaccctga tgacagctta aaacaagcaa cagacagtgc cttgcgttat
     2041 gtgattggtc atatgacgat ggatgaaatt ttaaccacag ggcgtgcaac cgtgcgtgaa
     2101 agaacgtggt cgacattgcg tgatattatc aaaacctatg atatgggttt attggtaaca
     2161 gacgtaaact tccaatatgc acgaccgcct gaagaggtga aagccgcctt tgatgatgca
     2221 atcaaagcac aagaagatga gcaacgctta attcgtgaag cggaagccta tgcaagaggt
     2281 gaagagccga ttgcacgtgg ccaggcacag cgtacgattg agcaagctca agcttacaaa
     2341 gaagcagttg tgttaaatgc gaaaggggaa gtggagcgtt tgagtcaatt attacctgaa
     2401 tataaagcct ctccagagct gactcgtgaa cgtttatata tccagacaat ggaaaaagtc
     2461 atgaaaaata cgccgaaagt ggtgatggat tcaagtggta ataatttgaa tgtattgcct
     2521 ttcgataagc taatgaattc ttcatcggta ataaaagcgg agcagcctgc ggcggcacaa
     2581 gtagttacac cacaagttgt tcagccgcaa gtacagccaa gaacacaaat tcagccgcaa
     2641 caagcagaac aacaagaacc aactcgtaag gggagatttt aatcatgcgt agattattag
     2701 tacctatact tgctgtagtt gcttttgttg tgctacaggc aatcaccatt gtaaacgagg
     2761 gcgagcgtgg cattatgtta cgttttaaca aagttcatcg tgatagcgat caaaaagtgg
     2821 tggtatatga accggggatt cactttaaag taccatttat cgatagttta aaggtattag
     2881 atgctcgtat tcaaactcta gatgggcaag aagaccgttt tgtaacagtc gagaaaaaag
     2941 atttattagt ggactcctat gtaaaatggc gaatcagtga ttttggccaa ttttatacct
     3001 ctactggggg ggattaccaa aaagcggcgg atttattacg ccgtaaggta ggggatcgtc
     3061 tgcgttccga gattggttct cgaaccatta aagatattgt atccggctct cgtggtgagt
     3121 taatggcagg agcccaaaaa gcgctgaatg ccggtgaaga tggagcagag cgtttaggaa
     3181 ttgaagtggt tgatgtgcga gtgaagcaaa tcaacttacc aaatgaagtt tcttcttcaa
     3241 tttaccaacg tatgcgtgcc gagcgtgatg cggttgctcg tgaacaccgc tcacaaggta
     3301 atgagaaagc agaggtaatt cgtgccgaag tggataaaaa agtggtgctg attttggcaa
     3361 atgcgaacaa aactgcccaa gccttgcgag gtgaaggtga cgctcaagct gcaaaactct
     3421 attcagagaa atttggtaac gagccggaat tttacagttt tattcgtagt ttaaaagcct
     3481 atgaagatag ctttgccgaa ggtcaaaata atatgatgtt attaaagcca aacagcgaat
     3541 tcttacgctt tatgcaagca ccgacaaaat aattgtatct ataaaacccg atcttgtatc
     3601 gggtttttaa ttttcatccc tattctgatc ataacaagcg gtaaattttg gctgattttt
     3661 tgcaaaaaat ttgaaaaaat tgaccgcttg cgatatgaag ttataatggg tttcgttatt
     3721 catcctaact aaaagaggca tttctatggg taaaagtgtt gctattcttg gcgctcagtg
     3781 gggcgatgag ggaaaaggca aaattgttga tttattaaca gatcgtgtga aatatgtagt
     3841 acgttatcaa ggtggacata atgcagggca taccttaatc attaatggtg aaaaaaccgt
     3901 tctgcgttta attccatcgg gtattttgcg tgataacgtg acttgtttaa tcggtaatgg
     3961 tgtagtgctt tctccagagg cattaatgaa agagatgggg gagctagaag aacgaggcat
     4021 taatgtgcgt gagcgtttaa aaatttctga agcttgcccg ttaattctgc cttatcacgt
     4081 tgcgatggat cacgctcgag aagcggcact tggtaaaaac aaaattggta caacaggacg
     4141 tggtattggc ccagcttatg aagataaagt cgctcgccgt ggtttacgtg tcagcgattt
     4201 attcgacaaa gaacattttg ccgcaaaatt aaaagatatt ttagattact acaacttcca
     4261 attagtcaat tattacaaag ttgaagcggt agattatcaa aaaaccttag atgatgtgtt
     4321 tgcgattgcg gatgtgatta ccggaatggt tgccgatgtg acaactttac ttcacgaagc
     4381 acgtgaaaac ggcgacaata ttttatttga aggggctcaa ggcacgatgt tagatatcga
     4441 ccacggtact tacccgttcg taaccagctc gaatacgaca gcaggcggtg tggcaaccgg
     4501 atcaggctac ggtcctcgtc atttagatta tgtattaggg attatcaaag cctactgtac
     4561 ccgtgtaggt agtggaccgt tcaccaccga actatttgac gatgtaggtg ctgaaattgc
     4621 tcgcaaaggt aatgagttcg gagcggtaac cggtcgccct cgccgttgtg gctggtttga
     4681 tgccgtagcc gttcgccgag cagtgcaaat caactccatt tcaggcttct gtatgactaa
     4741 actcgatgtt ttagacggat tcaaagagct aaaaatctgc gttgcctaca aaatgccaaa
     4801 cggaaaaatt gtcgaatatg cccctcttgc agcaaaagac tgggaaggtg ttgaaccaat
     4861 ttatgaaaca atgccgggtt ggtctgaaaa tactttccgt gtgacaaaac gtgaagattt
     4921 accacaagcc gcattagatt acattaaacg tatcgaagag ctggtcggtg tgccggtaga
     4981 tattctttct acaggtccgg atcgtgtcga aacgatgatt ttgcgtgatc cttttgcagc
     5041 ttaataaaag ataaaataaa gccatttagt gagatagagc tagatggctt ttttgtttga
     5101 accagtaaac tttactttgg cacaattttg tcacaataca gaaaatagtt ctttagcgtg
     5161 agtttttgag gctgtgtaag tatttgaatt taaaagaatt taatggcacg ccctaaagga
     5221 ttcgaac
//
LOCUS       DANLUA010000168         5224 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLUA010000168 DANLUA010000000
VERSION     DANLUA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            4..285
                     /locus_tag="PXB44_002162"
     CDS             4..285
                     /locus_tag="PXB44_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL5282601.1"
                     /translation="MTKSELIESLASKNPSLPIKMVEHCVKELLEQLTATLEEGERIE
                     VRGFGSFSLHYRQPRLGRNPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            322..609
                     /locus_tag="PXB44_002163"
     CDS             322..609
                     /locus_tag="PXB44_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL5282602.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            613..1809
                     /gene="lapB"
                     /locus_tag="PXB44_002164"
     CDS             613..1809
                     /gene="lapB"
                     /locus_tag="PXB44_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL5282603.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1833..2525
                     /gene="pyrF"
                     /locus_tag="PXB44_002165"
     CDS             1833..2525
                     /gene="pyrF"
                     /locus_tag="PXB44_002165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL5282604.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2608..2919
                     /gene="yciH"
                     /locus_tag="PXB44_002166"
     CDS             2608..2919
                     /gene="yciH"
                     /locus_tag="PXB44_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL5282605.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            3022..5184
                     /locus_tag="PXB44_002167"
     CDS             3022..5184
                     /locus_tag="PXB44_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL5282606.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
BASE COUNT         1749 a          832 c         1101 g         1542 t
ORIGIN      
        1 aatatgacta aatctgaatt aattgagagt ttagcatcta aaaatccaag tttaccaatt
       61 aaaatggtgg agcattgtgt aaaagagctt ttagagcagt taacggctac tttagaagaa
      121 ggtgaaagga tagaagtaag aggatttggt agtttttctc tacattatcg gcagccgcgt
      181 ttaggtcgca atcccaaaac aggagaaagt gttcttttag gcgcgaaata tgtaccacat
      241 tttaaagctg gtaaagatct aaaagagaga gttgatctgc tttaagtctt attaattcaa
      301 taatcgtagt aaggagatta tatgattaag tatattttag gtattttaat tgtgattgca
      361 gttattattg tcgctgttac gattggagcc aataatgatc aacttatcac ctttaactat
      421 atttttgctc agagtgaatt acgcttatca acgttagttg cgattttatt tggcttagga
      481 ttgattcttg gttggttggt aacgggtatt ttttacttaa aaattaagtt tcaaaacgtt
      541 gctttaaatc gtcgtgtaaa gcgccaagct cagcaaataa ctgaattaac gactccaaag
      601 gctgaataat caatgcttga attattgttc cttttattgc ctattgcagc actatatggc
      661 tggtatatgg gacaacgtag tgcaaagaag gatcaagaaa ccgctagtaa taagttctct
      721 cgtgagtatg ttacggggtt aaattttctt ttatctaatc agcaggaaaa ggcggtggac
      781 cttttcctct ctatgttaca aaaacaagaa tccgaaaatc atattgcatc tgaatcccaa
      841 tttgaagcgg agttaaccct aggtaatctt ttccgttcaa gaggtgaggt agatcgtgcc
      901 ttacgtattc accaaacatt agtgaatagc cctcattatt catttgatca aaaattatta
      961 gcaaaacagc aattagctaa agactttatg gttgcaggtt tctatgaccg tgcagaaaat
     1021 tattacattt cattagttga tgaacctgag tttgctaaat attctttatc tcagcttgct
     1081 gttatttacc aaaaaaccag agaatggaaa aaagcgatta atgttacaga aaaacggctt
     1141 cgaatagagc cggatgcaga taaaataccg ctttcacatt tttattgtga atatgcgcaa
     1201 ggggttaaat ttgatgataa agcggcattt attgaatcgt taaataaagc gttagaatat
     1261 tatcctcaat ccacacgcgc ttctataata ttaggtgatt tctatttaga agagggagaa
     1321 tatctaagag cattaagcta tttcgaacag attcttgtgc aagaccctga ttatattagc
     1381 gaagtgcttg gacgaattaa gcaaagctat atggctcttg aggacttaaa tggctatgag
     1441 ttatttctca ttcgagcaaa ccgagtaaaa cataatagta gtgtagatat tgctttaact
     1501 gaatttattg aagaaaaaga tggtatcaat gctgcacatt ctaaactata ccagcagttg
     1561 agtacttatc cgaatttaat tacttttcac cgctttattc gctatcaagc agattttgcg
     1621 gaggaaggaa atggtaagga aagcttagtt ttattacata atatggtggg aaatcagata
     1681 aaacgcagct tccaatatcg ctgtttaaat tgtggttatc aaagttatcg cttaatgtgg
     1741 caatgtcctt cttgtaatca atgggaaaaa attaagccgg tacaaagtat cgaaggtatt
     1801 attcaataaa gaaagaatct gaggaattga aaatgaacaa taaagtaatt gttgcgttag
     1861 actatgaaac tgaacgtgaa gcattagaat ttgtggattt ggtcgatccg tcgctttgtc
     1921 gtttaaaagt aggcaaagaa atgtttacaa ccttaggcac aaattttgtc aaacaattac
     1981 acgaacgtaa atttgatgta ttccttgatt taaaatatca tgatattcca aatacggttg
     2041 ctcgagcagt acgttctgct gctgatcttg gtgtgtggat ggttgacttg catgcttctg
     2101 gtggtttagc catgatggaa gaggcaaaga aaattttgga gccttatggc aaagaggcac
     2161 cattattaat tgcagtgacg gttctaacca gtatggaaga tttggatttg cttcaaatag
     2221 gtattaatgc ttcgccgatg gaacaagtta ttcgtttagc acatttagca caacgggctg
     2281 gtttagatgg cgtggtatgt tctccacaag aagttgaggt attacgtacc cattgtggtc
     2341 aagattttaa attagtgacc ccaggaatcc ggccaaaagg aacagatttt ggggatcaac
     2401 gccgagtgat gacacccaaa caagcagtag aaacaggctc agattttttg gttattggtc
     2461 gtcctattac ccaagctcaa gatccgcttg cggtattaaa atctattaac caatctatca
     2521 gttaattcat tgcattttta atgaaggcgg aaaaatttcc gcctttttta atcaaacaag
     2581 cggttatttt tgaggaaaaa tttgtaaatg acattagtct attcaacgga aacaggcaga
     2641 ataaagtccg aagtaataaa agaagaacgc cctaaagggg atggtattgt gcgtattcaa
     2701 cgtcaaacaa gcggtagaaa ggggaaaggt gtttgcctta tcaccggtct tgatttagag
     2761 gataaagcct taaattctct tgcttcagag cttaaacgta aattgggttg tggtggctca
     2821 tttaaagatg gagtaattga aatccagggc gataatcgag gattcattaa gcaaatctta
     2881 gaacagaaag gtttcaaagt gaaactggca ggtggttgag ttttaaacaa ttatcagctg
     2941 gcagttttta aataacttca ttttatttga aacttcattc ctacttttct gcctaaaact
     3001 cacgattaag gagaaaaact aatgtggaag aaaacgttga ttgctgtatt tttagcaagc
     3061 caatatgctt ttgcggtagg aaccgggaaa gaaccatcaa aaagttatac tgaaacagaa
     3121 ttaaaacaat taaaaaatga ttggttagcg atttatcaaa aagagctaca gtatgcagaa
     3181 aatttgcgtt taaaacagag agaaaatttt cagcaaatta attatttggc agatgcagcg
     3241 attagtcaga agaatttatc actttcaacg gagcagataa ttcagcgttt attactaacc
     3301 ttagcgggtt atccgttgga gatgaaagct gaatggaagc tgctaaatgc taaaataacg
     3361 ttaaaacaag ccacagaaag tgaagttaaa gcatttattt caaaatatcc tgattctttg
     3421 tatcaaaggc agttacagaa cttgatgttt gagctgctct atcgtgaaca gaaatttccg
     3481 gagttactgg aatatgctca aaatgtaaaa cagagtagtc ttgccgacca atgccgttta
     3541 ttttctgctc gttatgaact ggcagcaagt aaagctcaaa tcaatccgga agtttctcaa
     3601 gtaactgagc aaaaagcaga aaatgcagaa ctgcttgctt tggtgcaaga gtttgatcgc
     3661 ttttggcttg ctacgccgaa attaacttct gattgtgcga atttagaaag ttattggcgg
     3721 gatcaggggc ttaaaaccga tgaaaaaatg cgtttgaaag cggtcgaatt aattaaacaa
     3781 aatgcaaatg cagaattggc gaatttggcg gcaaatgtaa ataatgagaa tttgaaaatc
     3841 tggctatctt ccgttgaaaa ggtagcgaat aatccggcag atttgcaaaa ttttatcgaa
     3901 aatcaaccgc ttgattcccc attttatgct gataataaag cgattattca gcaactcttt
     3961 gctaaatatg tgcgaacttt atcggaaaat atggagaacc cgagtttcca gcagtatcaa
     4021 acgtgggcag agaagtatca actttccagc gaggaaatca atggctggaa aaatgctttt
     4081 atcactcgat tttttgataa cccagaacca acattccaat tttggcgtga tgagcaaatc
     4141 aagttgctca agacagacaa tttaactgaa cgccgtttac gaatggcaat ttggcaaaaa
     4201 gcagatttaa atgagtggct ggttctgcta tctcagcagg cgaaagaaaa agcagaatgg
     4261 cgttattggg ctgcgaaaga ggagaaaaac gaagcgaagc gtaaagttga ttttgaaagc
     4321 ttagctaaag agagaggctt ttatgctatg ttggctgctc aacaattagg cattgtttac
     4381 caaatcccga tgcttgaagt gcctgaattg acacaagcac agcttgaatt gtatgcaaac
     4441 caattagctc gtattcaaga gttgcgagag ctagggcagt ttgaccaggc aagaaaaata
     4501 tggattgact ggataaaaaa catcaagccg gaagcaggtg aaaatacacc aactaaaatc
     4561 cagttagccc tgataaaata tgccaaggat aaaaattggt atgatttggc ggtggaaggt
     4621 acaattcaaa caaaagcctt tgattatctt gatttgcgat taccgaatgc ctattcggat
     4681 tggttcgatt tacatctgca agataaaaaa atcagcaaaa cgtttgctca ggcgattgct
     4741 cgtcaagaaa gtgcgtggaa tgctcaggct caatcgcacg ctaatgcgag agggctaatg
     4801 caactgttac cgacaacagc acaaaaaacc gctaaagata acggtttaac cttcaaagat
     4861 ggcaatgatc tctttcaacc gtttaacaat attatgttag gcacaaccca tttaatggag
     4921 ttaaacgaaa aatatccgaa taatcggatt ctgattgcct ctgcttataa tgcaggagca
     4981 gggcgtgttg agcagtggct gaaacgtagt aacgggcagt tagcaatgga tgaatttatt
     5041 gccagtatcc cgttttatga aaccagaggc tatgtacaaa atgtgctggc gtatgactac
     5101 tattatcaaa tgctctatag cgatataaat gataaaaatg gattaaaaat gttctatcaa
     5161 gaggaattaa caagaaaata ttaaaaaatg cttgtgtttt tgtgtagaac cgctataatg
     5221 cacc
//
LOCUS       DANLUA010000169         5133 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLUA010000169 DANLUA010000000
VERSION     DANLUA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXB44_002168"
     CDS             complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXB44_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL5282607.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXB44_002169"
     CDS             complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXB44_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL5282608.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3299..3619)
                     /locus_tag="PXB44_002170"
     CDS             complement(3299..3619)
                     /locus_tag="PXB44_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL5282609.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3740..4042
                     /locus_tag="PXB44_002171"
     CDS             3740..4042
                     /locus_tag="PXB44_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL5282610.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4067..4441
                     /locus_tag="PXB44_002172"
     CDS             4067..4441
                     /locus_tag="PXB44_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL5282611.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4617..4706)
                     /locus_tag="PXB44_002173"
     tRNA            complement(4617..4706)
                     /locus_tag="PXB44_002173"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4670..4672),aa:Ser,seq:gga)
     gene            complement(4798..5109)
                     /locus_tag="PXB44_002174"
     CDS             complement(4798..5109)
                     /locus_tag="PXB44_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5282612.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
BASE COUNT         1534 a         1119 c          925 g         1555 t
ORIGIN      
        1 ttactttaga tacagttctc aatacaaatt atgcgtgttc ttttaagcgt ttaattaatt
       61 gggcatttgc cggtgggaaa gctccctcat ctaaatccga ttgtgcgatc caaaaacctt
      121 cttgcccttc tcttccgtaa ggttcattta cccactcttc caccaaataa aagaaaaatt
      181 caatgatttt tgttggatat tcaaagcaaa aattttcata cggaaaagcg cttaacactt
      241 gaataccaat ttcttcttct aattctcgtt ttagtgcttc ttcaggggtt tcattgggat
      301 cgactttccc cccgggaaat tcaagagact gagcaaaatc ttgcccttct aaacgttggg
      361 ttaagtaaat ttgcccaaat tcgttacgaa taatccctgc tgagacttga ataatcggtt
      421 ttgacatagt ttccttcact ctttctataa attcaagcgg tcatattaag cagaaaattt
      481 gcaaattcct gactgaatat gaccgcttgt taattacgaa tatctcgctt tgttaccatg
      541 gcaatgctta tattttttgc ccgaaccaca agggcaaggg tcatttcgac caatatttaa
      601 gtttgccaac tcttcttcac ttaagctctc tgcgctattt tcttctctac taccagcagc
      661 agaatcggta gcttgtgtaa ctggcgtact tgcagcgtcc atttcagctt gctggcgttg
      721 cgcttcttcc acctcttctt tgctacgcac ttgaatacgg cttaaaatgc taattacatt
      781 caattttaag gtatcaagca tattggtaaa catcgcaaaa gattcctttt tatactcttg
      841 cttcggatct ttttgtgcat aaccacgcaa gtgaatacct ttgcgtaaat aatccattga
      901 ggctagatgc tctttccaaa gctcatctaa gttttgtagc attacacctt tttcaaaatt
      961 acgcattacc tctgcgccta ctatttcttc cttcgcattg tactctgcaa tcgcaatatt
     1021 taagatacgt tcacgcaaag tctcttcgtg caagtctttt tcagtttcaa gccagtgaga
     1081 aatcggtaaa tccaacccaa attcacggcg taaacgctct tctaagcccg gcacattcca
     1141 catttcctcg atagattgag gcggaatata ttggttaatc gtgctttcaa atacatcttg
     1201 gcgaatgctt tcgatcattg aggcaatatt atctgtttct aatagatgat tgcgttgttc
     1261 gtaaattact ttacgctgtt cattggcaac atcatcatat tgtaataaat gcttacgccc
     1321 atcaaagtta tgcgcttcta ctttcgcctg tgcagaggca atcactttgg ttaacagttt
     1381 agactccatc gcttcacctt cttcggtaaa ggctttacgc atcatattta atttaccttc
     1441 attgaggtaa attctcatta gggcatcatc taaagagaga tagaaacgtg atgaccccgg
     1501 gtcaccttga cgaccagaac gacctcgcaa ctggttgtcg atacggcggg attcgtggcg
     1561 ttctgtacca ataatatgta aaccaccggc ttgcattacg atgtcataac gctcttgcca
     1621 cgcagcttta atcgcatcaa tttgttcttg tgtcgggttc tcaagcttgg ctacttccat
     1681 tttccagtta ccgccaagca caatatccgt accacgacca gccatattgg ttgcgatagt
     1741 aaccgccccc ggagctcccg cttccgctac aatttcagcc tcttgagcgt ggaatttcgc
     1801 atttaatacg ttatgagcaa taccggcttt ggttaattct gccgataata actctgattt
     1861 ttccaccgat gctgtgccaa ccaaaacagg ctgtttacgc tcgatacagt ctctaatttc
     1921 agtaataatc gccgcaaatt tctccgcttc gcttttaaac attaagtctg tacggtcatc
     1981 acggatcacc ggtctgttgg ttggaatcac caccgtattt aagccataaa tttgctggaa
     2041 ttcaaaggct tcggtgtctg ccgtaccagt cataccggct aatttttcgt acaaacggaa
     2101 atagttttgg taagtaattg aggcaacagt ttggttttcg ccctgaatag ccacacgctc
     2161 tttagcttca atcgcttggt gtaagccgtc agaccaacga cgtccagcca ttgtacgacc
     2221 ggtgtgctca tcaataatca caatttcacc gtcttttaca atgtaatcta cgttgcgttc
     2281 aaataattta tgggcacgca atgctgcact cacatggtgt aacagcgaaa tacgagcagg
     2341 gtgatataaa ctttcgccct cttccattaa ccccatttga gttaagaaat cctctacttt
     2401 caccatacca cgctcggtta aatgagcctg tttgtttttc agatctaagg taaaatcgcc
     2461 ctcaccggtg tactcttcac tgtcttcttt atcttgagca atcaaatgcg gaatgatttg
     2521 atctaccgct tgataaattt gggtcgcatc ttccgcaggc cctgaaataa ttaacggcgt
     2581 acgagcttca tcaattaaaa tagagtctac ttcatcaact aatgcgtagt gtaactcacg
     2641 ttggaaacgt tcgtttttat cgtgagctaa gttatcacgc aagtaatcaa aacctaattc
     2701 gctgttagtc gaataggtaa tatctgcttg gtatgcagca cgttttgcat cggaaggtaa
     2761 ccccggaata ttcactccaa cggttaagcc taaaaactca aataacggac ggttggtttc
     2821 cgcatcacgg cgggcgaggt aatcattcac cgtaacaaca tgaacacctt taccggtcaa
     2881 agcattcaaa taacaagata aagttgcagt tagggtttta ccttccccag tacgcatttc
     2941 tgcaatatta cgccccgtta gcaccatacc gccgatcagc tgtacatcaa atggtcgcat
     3001 acctaatact cgtttgcttg cttctctcac tgtcgcaaaa gcttcaggta ataggctatc
     3061 tagcgataca ccatctgcaa ggcgttgctt aaactccgct gttttatcct ttaacgcctc
     3121 atcagataat tgctcgaagg ttggttcaag cttattaatt tgtgccactc gctttcttaa
     3181 acgttttaaa gtgcgatcgt tgcttgagcc aaaaatagaa gtcagtaatt ttgaaatcat
     3241 tgtgttattc ctaaatataa aaatgggtta atcaattaat ggtaaaatta aattatattt
     3301 aggtttgtgg cccggctcga atggggtaag cattttgtct atccaagcgg taggagtttg
     3361 caaaaaattc gcaaaaatgc accgcttgtg gtttaatttc cacaatttta tggattaact
     3421 ctgcaataaa aagcgattgt tgctcaatat cattagcgga ttgagcatat tgaagaaaag
     3481 attgattaag aacggtttcc tgttcacttt cattggcgta cgcaacctga atttcaggta
     3541 aagcaagtat cgcaaccatt cccaaaaaga gttgcgacca aaaaggcgtt cgatgaaagt
     3601 gttttaaaaa taagctcatt aaattaagat aaatgaaaaa attgggctaa ttatacgcta
     3661 aagtaaagcc gaatgagtaa attattgggt caagtatcgt aaaatcaagc ctaaaataag
     3721 acgaaatgta aagaaacaga tgaaaaattc agcacctaaa aagattaatg acattttaca
     3781 gaactcaagc ttaagccgta ttgtcgaaaa agcaaacttc attaacgatt taaaccaaaa
     3841 aattcagaaa agatggccag aatcttatcg tgggctttac cgagtagtga atttagttga
     3901 taatcagctt attgtgagcg ttaagaatgc aaccgtacgg caaggtttaa tcttgcaaca
     3961 aaatatgtta ctaaaacaga tacaaaccga tttacctgaa ataacccaac ttgaatttag
     4021 agtaaatccg agtgttacat aatttaattt tgaagaggaa aatagtatga aaaaaatcgc
     4081 actcattggt tttaccaccg caatgctagg ggcttgctca ttgctaccac aaaaaagcgt
     4141 tagcggtact tatgcaggtg aattaccgtg tgctgactgt gagaaaatcc aagcccagct
     4201 aaccttaaat gcagataaaa cctaccaata tgatacggta tattttaaag ataaaaaaga
     4261 atacacttac cgagacaaag gcacatacag ttgggaaccc aataagccaa atgtgattcg
     4321 tctggaaaaa gcatcaggca atcttgcctt ccaagtgtct gaacaacacg ttgaactttg
     4381 cgacccaaat ggcaacacgg taaaaaacag caataactat aaactaggta aagtacgtta
     4441 aaagcagata aaaaggattc ttaggaatcc tttttctttg taatctatcg ccgatcgcat
     4501 aaaaaaaccc ccaagcaatg tttgctcggg gataacctaa aaatgaaaaa gtattatcta
     4561 ctttaactta accaaggaaa tatctatgaa aacagtaagg aatctttcag tatgaatggc
     4621 ggtgagaggg ggattcgaac ccccgataca gtttcccata tacacgcttt ccaggcgtgc
     4681 tccttcaacc actcggacat ctcaccgaat caacaaaaag atggcgtaaa tatagcaatt
     4741 ttacacggct ctttcaagta ttttcttcac aaaataaaat aaatcatttt tatccactta
     4801 ttgcccaaca cctaagcaat ttagcgattt aggtacaagt ccgccagtcg cttcccattc
     4861 accttgggta tataggtgca aagcaagtgc gtgaacgcca ttagctaatt cctcagctaa
     4921 gcaagcatag acagcacgat gacgtgcaac tgttctctgc ccttcaaatt tatctgacac
     4981 caaagttaat ttaaaatgcg attctgctcc tctacctgag ctgtgcatat agctttcgtt
     5041 ttcgacatta aagaaaatcg gctgaaattc tgtcttaatt ttttgaataa ttgtctgttc
     5101 taccgacatt ttttgctctc gttcattgaa aat
//
LOCUS       DANLUA010000170         5102 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLUA010000170 DANLUA010000000
VERSION     DANLUA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..633)
                     /locus_tag="PXB44_002175"
     CDS             complement(<1..633)
                     /locus_tag="PXB44_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5282613.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXB44_002176"
     CDS             complement(760..1353)
                     /locus_tag="PXB44_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5282614.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXB44_002177"
     CDS             complement(1364..2410)
                     /locus_tag="PXB44_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL5282615.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXB44_002178"
     CDS             complement(2407..3090)
                     /locus_tag="PXB44_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL5282616.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXB44_002179"
     CDS             complement(3148..3336)
                     /locus_tag="PXB44_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL5282617.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXB44_002180"
     CDS             3461..4117
                     /locus_tag="PXB44_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282618.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXB44_002181"
     CDS             4129..4503
                     /locus_tag="PXB44_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282619.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..>5102
                     /locus_tag="PXB44_002182"
     CDS             4655..>5102
                     /locus_tag="PXB44_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5282620.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDIISHISFGFWVNLFNYDIKRNQYVAYWQSVLKPIFGH"
BASE COUNT         1481 a         1041 c          984 g         1596 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatgcggac gtaactataa
     4981 tgaaaatgac ataatttctc atatttcttt tggattttgg gtaaatctgt ttaattatga
     5041 tataaagcgt aatcaatatg ttgcttactg gcaaagcgta ttaaagccta tttttggaca
     5101 ta
//
LOCUS       DANLUA010000171         5084 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLUA010000171 DANLUA010000000
VERSION     DANLUA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            165..2072
                     /gene="parE"
                     /locus_tag="PXB44_002183"
     CDS             165..2072
                     /gene="parE"
                     /locus_tag="PXB44_002183"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL5282621.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
     gene            complement(2146..2751)
                     /gene="plsY"
                     /locus_tag="PXB44_002184"
     CDS             complement(2146..2751)
                     /gene="plsY"
                     /locus_tag="PXB44_002184"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL5282622.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            2821..3177
                     /gene="folB"
                     /locus_tag="PXB44_002185"
     CDS             2821..3177
                     /gene="folB"
                     /locus_tag="PXB44_002185"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL5282623.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            3174..4076
                     /locus_tag="PXB44_002186"
     CDS             3174..4076
                     /locus_tag="PXB44_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5282624.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            4090..4989
                     /gene="cdd"
                     /locus_tag="PXB44_002187"
     CDS             4090..4989
                     /gene="cdd"
                     /locus_tag="PXB44_002187"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL5282625.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
BASE COUNT         1459 a          937 c         1208 g         1480 t
ORIGIN      
        1 aatgaataag caaataggga aattaaaact cattttttcg atgtagatcg caaaaatagt
       61 ttttattgca taacaacagg tcatttttca ccaaaatttt gcaaaatagc tgaatattcc
      121 ttaaaattca gccttatttt attttaagcc taagagaaag cataatgtct gaaaaacgct
      181 atggggcgga tgaaattacc gttctgaaag acctcgagcc ggtgcagctt cgcccgggaa
      241 tgtacacgga taccacacgt ccaaaccatt tggggcagga ggtgattgat aatagtgtcg
      301 atgaggcgtt gtccggcttt gcagataaaa ttgatgtgat tcttcatgct gataattcgt
      361 tggaagtggt ggataacggg cggggaatgc cggtcgatat tcattccacc gagaaaattt
      421 caggggtgga gcttatctta acgaaattgc acgccggcgg taaattttcc aataaaaact
      481 atacgttttc cggtggtttg cacggggtgg ggatttcggt ggtaaatgcc ctgtccgaac
      541 gggttgaaat tcagattaaa cgtggcggag aagtctattc gattgccttt gccaacgggg
      601 taaaagtgga agatctgacg gtaattggta cttgcccgaa aaaacagacc ggcacgacag
      661 tgcgttttta cccgaaccca aaatattttg atagccctcg tttttcggtg agtcgtttac
      721 gccatttgtt acgagccaaa gcggtgcttt gcccgaaact caccatcaat tttattgata
      781 aaatcaacaa taccgaagaa agttggtatt acgaagacgg cttatctgat tatttgagcg
      841 aggcattaaa cggttatgaa accgtgccga atccaccgtt tattggtgat gttactgctg
      901 aaacagaggc ggtaagctgg gcattaactt ggttgccgga agggggcgag ttagtagcgg
      961 aaagctatgt gaacctgatt ccgaccgcac aaggcggcac gcacgttaat ggcttgcgta
     1021 acggtttatt aaaggcgatg gtggagtttt gcgaaatcca taatttattg ccgaaagggg
     1081 taaaattaac cgctgatgat gtgtggaacc gttgtgctta tgtgctttct ctgaaaattc
     1141 aagagccaca atttgcaggg cagaccaaag agcgtttgtc ttcccgtcaa gcctcaagct
     1201 atgtggatag caccttgaaa gatgccttta gtttatggct taaccaaaat gtgcagacag
     1261 gtaaattaat tgctgaaatg gcaattagtt ccgcccagag ccgtatgcgt gcggcgaaaa
     1321 aagtggtgcg taagaaattg gtgagtggtc cggctttacc gggcaaactg gcggattgta
     1381 cagctcaaga tctcagccgt accgaacttt tcttggtaga aggtgattct gccggcggtt
     1441 cggcaaaaca agctcgagat aaagagtatc aagccatttt gccactgcgt ggtaagattt
     1501 taaacacttg ggaggtttca tcagaccaag tattggcttc acaagaagta cacgatattg
     1561 cggtagcctt gggaattgac cctgatagcg ataatttaga agagttgcgt tatggcaaag
     1621 tttgtattct tgccgatgcg gattcagacg gtttgcatat tgccaccttg ctgtgtgcgt
     1681 tattccttcg tcatttcccg aaattggtgc aaaacggaca cgtttatgtg gcaatgccac
     1741 cactttatcg cattgatatc ggcaaagatg aggtgcatta tgccttagat gaggcggaaa
     1801 aagaggcgat tttagctcgc cttgccaaga aaaaaggaaa gccaaatgtg caacgcttta
     1861 aaggcttagg cgaaatgaac ccgagccagt tgcgtgaaac tacgatggag ccaagcactc
     1921 gccgcttagt acaattaacc tttgatgaca cggtagaaga gggcgaattg aatacctttg
     1981 aaattatgga tatgctattg ggtaaaaaac gagcggaaga tcgtaaacaa tggctacaaa
     2041 atcgaggaga tgaagcagat ttagcggttt agtcgagaag acaagcggtg atttttccgc
     2101 aataatttgc aaataggcat agcgaacggt tcactatgcc tatttttatg ctctattttt
     2161 cttgaattta tcccacagtt tgtcttcctg cccacgccat aagcgttgaa tattatcgtg
     2221 atggcggtaa accaataggc aacaaactaa ggcgacgggg aaagtaaatt gtggctcgaa
     2281 ccaccaaatg taaaacggta cgaccaaggc agtgaagacg gcacttaaag aagaatagcc
     2341 ggagattaaa aacaccagta gccaagtgcc gatagcaccg cctgcaacaa taaagcttaa
     2401 tgcggagatt gcaccaaatg cggtggcaac gccttttccg ccacggaatt ggaagaaaat
     2461 cgggaaaata tgcccaagac aaaccgctaa ggcgatgaaa ccgagttgtg agggagagag
     2521 ttcaaaataa atccccatcg ccaccggtaa tgtgcctttt aaaatatcga ataaaagcac
     2581 acctaatgcg gcacttttac cgccaattcg tagtacgttc gttgcaccgg cattgtgtga
     2641 gccgacttct cttgggtcag gcaagcctgc cagcttacaa aaaatgaccg cactggaaat
     2701 cgagccgagc aaataggcgg ttaagatcaa taaataggga attaaattca taaattttcc
     2761 tgattaaatt tatcttgtgc tatattttac agaaattcag aaaataaaac aggacgaaaa
     2821 atgagtgata aggtttttat tcgagaacta accgcttttg cttccattgg ggcttacaat
     2881 tgggagcata cgattaagca acgcttggtt tttaacattg atatggcgtg ggatttttcc
     2941 aaagcggcag aaaccgatga tgtacaatat tgcttgaatt atgcggaagt atcgcaaaaa
     3001 gtgctggatt ttgttgaatc tcaacctttc aaattagttg aaactgttgc ctatcaactg
     3061 gcggaactat tacaaaaaga gtatcaactt tcttggattc ggattgaact gcataagccg
     3121 aaagcggttg cccaagcaag cagtgtaggt gtgattgtag aacgaggtca tcaatgatct
     3181 catcatcact gaaattgggg tatttcagtt taatcgttgc tactcttttt tggggtggga
     3241 attttgtatt aggcaaagtg ttaagtgggg cagttcagcc tattacctta acctatttgc
     3301 gttggttgcc ggctttttta attttagttt tgctcttttt tcgcccaact tggcgggtaa
     3361 tgcacttatt aaaacctcgt ttaggtgtga tgtgggtatt aggtctgctt ggggtaattt
     3421 tattcccggc taccttatat gaaggtttaa aaacgacaac agcattgaat gcgagtctct
     3481 atttagctat ttcgcctgta ttggtgctat ttttaaatcg gctcgttttt aaggagaaaa
     3541 tcagcccgat tattctaagt ggggcaataa tcagtttgct gggagtgctg tggctattga
     3601 tgcaagggaa gctaactcgc ttattcagct tggcaattaa tcaaggtgat ttatgggcga
     3661 ttgcttctac tataagctgg gcggtttatt gttgtgtgat tcggctacgt ccacaaggta
     3721 taagcaatac ggtgtttttg accgtattag tggggctggc agttctaacg ttaacgccat
     3781 tttttatcta tgaatatgta gcagaatctg caaaaaattt agcaaatttg accgctttac
     3841 aatggagtgg gataggctat ttggttatcg gcccttctat tctctcttat gcgttttgga
     3901 atttcggcat tgcaatagtc ggttctgcta aaggagcggt aatgactaat tttacgccgc
     3961 tctttgcagc aatgttcagt attattttat taaatgaatc agttcatact taccatatta
     4021 ttagtgccat actgattacg gcagggatac tgatttgcag ctataaaaaa cattaaacgg
     4081 aggtacagta tgtttcgaac agacattaca gagcgttttg aacaattaaa agcagcgaaa
     4141 aactgtgaag ttacacaagc tattattgag caacttatca agcaagattt tcacgggcag
     4201 ctctcttacg aagtggttga tgaactctgt gaaaaattta aacgctctcg tgttgagttg
     4261 gctttatatt gcattgccat tgctgcctgt tatgcggtta caccagtttc cgattttaat
     4321 gtaggggcgg ttgcgattgg caaaaacgga gatttctatt ttggggcaaa ccaagagttt
     4381 tccggggaat gtatgcaaca atccgttcac gcagagcaaa gtgcaatctc gcacgcattt
     4441 ttagcagggg aaactttgat aacagatgtg gttgtgaact ataccccttg tgggcattgc
     4501 cgccaattta tgaatgaatt aaacagtgcg gcaagcttaa aagtacattt accgcatagc
     4561 caaaataatt tgctccatag ctatttgccg gatagttttg gaccgaaaga tttagggatt
     4621 gagaaagtct tatttgatga acaaccgcaa caatttgagg ttaagggcga tcatttacaa
     4681 caagcggcaa tttttgctgc caataatgct tatgcccctt acagcaacgc atttagtggg
     4741 gttgcactgc aagtaggtga gcgtattgtt tgtggtaaat atactgagaa tgcagctttt
     4801 aatcctactt ttttaccgtt gcaaagcgcg ttgaactatc gattattttt agggcttgcc
     4861 gatattcccg ttactcgagt tgtgctggct gaggcgaaag gctctctcag tagttatgca
     4921 atgactcaag cattggcaaa atcactattt gggttagaga ttgaatacct ttcgctacaa
     4981 gcggtgtaat tttgatttat ttttgcaaag gtgctgtatc tttaagcaat ttatttttta
     5041 gtgttaaatt ttttgatgag cttcacactt ttgcaacaat tata
//
LOCUS       DANLUA010000172         5062 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLUA010000172 DANLUA010000000
VERSION     DANLUA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            124..2760
                     /locus_tag="PXB44_002188"
     CDS             124..2760
                     /locus_tag="PXB44_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5282626.1"
                     /translation="MDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNP
                     VEKEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLE
                     KVIQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNN
                     VFAINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESK
                     KQPGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIG
                     VADEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEK
                     GVDNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLD
                     GENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSG
                     LIANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAK
                     RGLIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQ
                     SALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEH
                     LNQKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTAL
                     NIGLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMP
                     NQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSI
                     SPYIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMN
                     LDYSRVKSANLFGWKVNVGFSF"
     gene            complement(2901..3338)
                     /locus_tag="PXB44_002189"
     CDS             complement(2901..3338)
                     /locus_tag="PXB44_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL5282627.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(3374..4294)
                     /locus_tag="PXB44_002190"
     CDS             complement(3374..4294)
                     /locus_tag="PXB44_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL5282628.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            complement(4310..4999)
                     /gene="ung"
                     /locus_tag="PXB44_002191"
     CDS             complement(4310..4999)
                     /gene="ung"
                     /locus_tag="PXB44_002191"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL5282629.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
BASE COUNT         1679 a         1029 c          940 g         1414 t
ORIGIN      
        1 agcatttcta gttttttatc tattgcatac gctacagaat caatagagaa tccacaacct
       61 atcattcaat tatctgagag cctcagctca aaatatagtg gcaagggtgt taaactaggc
      121 gtaatggatg agggcttcat ggttaaacac cctcgccata gttcacatct tcatcctctt
      181 attcatcaat taacaacacc cgaaggcgaa gtaagaatct acgatgcaag ttaccctcaa
      241 ttcgaggtaa accccgtaga aaaagaagat ggaattgatc ttattccttc tcttgaaaca
      301 cacggtgcag gtgtagctgg tattattgcc gctcaagcag ataaaactct tggtgatggc
      361 tatagtggtg gtattgcaaa gggagctgag ctttatgttg caacgaagtc atataaacga
      421 actttagaaa aagttattca agatgctaag aaagaattag aaaatgcaaa agacgaagag
      481 gatgaaaaaa cgccaagtct ggatcaaatg gcaaagaatg acttattagc aagcaaagaa
      541 aaagaaatgg caattgaacg tgctgaatgg gcatccgggc tgaataaatt actagataat
      601 aatgtttttg caatcaataa tagttggaat cctttttcta ttagcgatga catcaatgtt
      661 gtagataaat tttatcaatc tatcaagcaa aataagcaca atccgctact tcaagctatt
      721 atgagagcca aaaatagcaa caccttatta gtctttgctg ctggtaatga gagcaagaaa
      781 cagccaggcg taatggctct tctaccacgt tactttcccg agcttgagaa aaatttaatt
      841 tctgccgttg cggtagataa agaacaaaaa attgcttcat actctaacca ctgcggagca
      901 agtaaaaact ggtgtgtagc agcaccgggc gatttacatg ttttaattgg cgttgctgat
      961 gaacataaaa aaccacaata tggcttaaca aaagagcaag gtacgtcatt ttcagcacct
     1021 gctataactg cttcattagc agtattaaaa gaacgctttg attatctgac tgcgactcaa
     1081 attcgcgata ctttattgac taccgccact gatttaggtg aaaaaggggt cgataatgtt
     1141 tatggctggg gtctcattaa cctgaaaaaa gctgttaatg gcccaactca attcctaaat
     1201 gatgaaacga ttaccgtaac tcgtgatgac cattggtcga atccactcgc cagccaattt
     1261 aaaattacaa aaaaagggga caaatcactg catttagacg gcgaaaatca tttagatacg
     1321 gtagcagttg aagaaggtag attggcctta aacggtaaaa ctaaggtgaa aacaatcagt
     1381 aaccatgcaa atttagctgt caatggaacc gaagttgagc aaaattattc ttcgtctgga
     1441 caaagccaat tagaagtgtt gggcaaatct gggctaattg ccaatgctca agccaatata
     1501 catttagccg gctcgttaaa aattgatgat aaattaacag aaaaaaccga agcaggtgat
     1561 gtttcagcga ccgtagtaca gttgaaagat aaagcaactt atcaaggcgg atttacacaa
     1621 ttagtcgaaa acgagaattt agccaaacga gggttaatcc aagatctcta tttcaaagag
     1681 agcgaaatta tcgcaaaagt gaataagcca ctaactgatg agaaagccga tacaaacggg
     1741 caagccggtt tagcattatt aaatgcgtta agaactacac ctattgccta tcgccgtagt
     1801 tggtataacg gttggctgca atctgcactc gaacaacgta aattagataa tctgcactat
     1861 gctgtatcta acaatattta tgcagatagc cttgaattgc tacgtagcca aaatgccaaa
     1921 ggcttaaccc aagcacagca acatttgttt acggcatacc atacaccact gcaaacgact
     1981 gtttgggctg aacatttgaa tcaaaaacaa tcagcctcat cgaagcatac cgatgttaaa
     2041 caccaccaat cacaattggg agtaaaccac aaactggcag ataaaacggt acttagtgca
     2101 acactttcac agcaaaaaaa ccgcttagaa aaaccatttg cacaagctac cttaaaacaa
     2161 acagcattaa atattggctt acgttatcat ttagataacg cttggtttag tgaagctaca
     2221 ttgcaatttg ctcgccagaa atatcaacaa agccgtcgat ttgctagcca tcaactaggt
     2281 actgcagaaa ccagaggttc aacgctcggt ggtgaaatgc ggatcggtta tcaatttatg
     2341 ccaaaccaat ggataattga accaagcctt ggcgtacaat ggattcaaac caaaatgaat
     2401 ggtttaaatg aaagtggcga acttgcgact caaacggctg caatgcgtta tcgtgatgtg
     2461 aatattgttc caagcgtgaa attacagcga actttccaac ttgagcaagg ctctatttcc
     2521 ccttatatcg ggctaaatta cttgcataga ttaaatggaa aaataaccaa aattaccagt
     2581 aacattgcag gaaaaacact gcatagtgaa gcaacgacga aacgtaatcg ccaattaaac
     2641 ggcgaagtcg gggtgaaact gcattataaa aattggttta ctgcgatgaa tttagattac
     2701 agtcgagtga aatctgcaaa cctatttggt tggaaagtaa atgttggctt tagtttctaa
     2761 cttattatgg tgggacacaa gcggttaatt tagggtgaaa atttgcaaat ttcactctat
     2821 tttcaaccgc ttgagagaaa aaaccttgaa caactgttca aggtttttgt ttagtgttct
     2881 atgactaaat aagaaaaaac ttattgctta cggacaaata aggtaattcc ctccactttc
     2941 tggacttcaa tcttatctgc tacttgtaaa ttattaccta taacacgcca agtcgtatca
     3001 ccaaattttc ctcgagcgat gccattaggt aagatttcaa ccacaaagcc ttgagctcct
     3061 agcatagcat gctctcgaga gttaagggaa ctgctctgat cctctttctt gtcttttcca
     3121 tgctgatatt tccaccaaat aagactaaaa atagtcgcaa ttatcgcaaa taacgcaatt
     3181 tgtaacgctt gccctaatgt aaaaagtgca acgatgaaag caagaataag cgaggcaaat
     3241 ccccaccaca taataaagac acccggtaat actaattcta atgcaagtag tacaaagcct
     3301 aatatgctcc agccaatcca attaaaaaac cagtccatac tccctccata tactcaatta
     3361 atccggtttt aggctatgag cttgttttat tgctttttaa aatttcagca ataccagcaa
     3421 cagaaccaat caaatttcca gcttctaacg gcataagtac aactttactg ttatctgaag
     3481 aacctatttc tttcaaagct tcggtgtatt tttgtgcaat aaaatagtta atcgctgtag
     3541 tatcgccttt agcaattgct tcagaaacca tttgagtggc tttagcttcc gcttctgcgg
     3601 ctcgttcacg agcttctgcc tgtaagaatg cttcttgtct ttcaccttct gcttttaaaa
     3661 tacgagattg tttttcccct tcagcccgca gaatttcagc ttgtcgaatc ccttctgctt
     3721 caagaatatc agcacgttta ttacgctcag ctttcatttg agcgttcatt gccgcaacca
     3781 gctctttcgg tggacgaaca tcccgaatct caatacgggt gacttttact ccccaaatat
     3841 tagtcgcctc atcgacaatg gaaagtaatc taccgttaat taaatcacgc tgagagagca
     3901 tatcatctag atccatagag ccgagcacgg tacgcatatt tgtcatggtt aaattaacaa
     3961 ttgcttgctc taaatgattc acttcatagg cagctcggcg agcatcaact gtttgaacaa
     4021 aacatacagc atcaatcgca acacttgcat tatcacgtga aataacctct tgcgatggaa
     4081 tatctaaaac ttgctccatc atattcattt ttcgcccaat acgatcaata aaaggtacga
     4141 caatgttcaa ccccgggctt agcgttttag tataacgacc aaaacgttct actgtccaat
     4201 gatagccctg tggcacaatt ttaatagtcg aacttaatgc aactaaaaca agcacaacaa
     4261 atgcaatact aacaatcggt aaatcaaaat tcatcgtttg ctccttaaac taatgtgttg
     4321 aagaataact aacgtctact gataattgcc aattaattgg ggatattcct tgagaaatca
     4381 agtagttatt ggctttagaa aaatgtttac aaccaaaaaa tccacgatat gcggaaagtg
     4441 gtgatgggtg cggagaagct aacacacaat ggcgattacg atcgataaat tgcccctttc
     4501 tctgagcgtg gcttccccaa agtagaaaga ctaaattatc acggtgttga tttaattgtg
     4561 cgatcacttt atcggtaaaa atttcccaac caatttgtga atgcgaaccg gctctacctt
     4621 gctcaaccgt taagctggtg tttaacataa ataccccttg ttttgcccag tcaattaaat
     4681 agccatgcgg tggaatttga aatccttcaa tatcttcaga taattcttta tacatattga
     4741 ccagagaagg tggcggggca accggcggtt taactgaaaa ggctaatccg tgggcttgat
     4801 ttgggccgtg ataaggatct tgccctaaaa ttactacttt cacgtcacta aattcggtta
     4861 aggcaaaagc actaaacacc tcgttttgcg gaggatagat gactttgccg gctaatcgtt
     4921 cgttatgtac atattgtaaa atttgttgaa aatacgcttt ttgtttttct tcgcctaaag
     4981 cttctgtcca agttttcata aaaccaccaa gtgtatattt tttacccaac caactcaatc
     5041 atgtctaaaa atggttaaat tt
//
LOCUS       DANLUA010000173         4988 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLUA010000173 DANLUA010000000
VERSION     DANLUA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1080)
                     /locus_tag="PXB44_002192"
     CDS             complement(<1..1080)
                     /locus_tag="PXB44_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL5282630.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFY"
     gene            complement(1090..2274)
                     /gene="neuC"
                     /locus_tag="PXB44_002193"
     CDS             complement(1090..2274)
                     /gene="neuC"
                     /locus_tag="PXB44_002193"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL5282631.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2275..3534)
                     /locus_tag="PXB44_002194"
     CDS             complement(2275..3534)
                     /locus_tag="PXB44_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5282632.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITPTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(3594..4631)
                     /gene="neuB"
                     /locus_tag="PXB44_002195"
     CDS             complement(3594..4631)
                     /gene="neuB"
                     /locus_tag="PXB44_002195"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL5282633.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(4631..>4988)
                     /locus_tag="PXB44_002196"
     CDS             complement(4631..>4988)
                     /locus_tag="PXB44_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5282634.1"
                     /translation="DKTALVSKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKSLVEH
                     GCFIGSHCNISTNTTLNGDVIVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIRNVEP
                     RTVVAGVPAKYIKDIK"
BASE COUNT         1561 a         1055 c          833 g         1539 t
ORIGIN      
        1 ataaaaaata gtagataaaa tcgagcaaag cgaaattagc ttatttttgc cataaaaaaa
       61 caaataattg actaaaatga ggtaaggcac aaccaacatt gtcgcaatta aaaagagaat
      121 aatgtagtat tttgttccta caaattggct acccagaatc cataccacta attcttccgg
      181 aataaaccac atcacaatag caggtaatgg tacaataaat aaggcataca acgcccattt
      241 gtggatatgg ctgacattaa tttttttctg ctttaaggct tcataaaaat aaggcagtgt
      301 tgctttattt aaggcttgaa ccacaatggc tagaatcatc gcaatttgag cccccattgc
      361 atataaacct aaatcctctt cgctaaattt atggtaaata aatactctgt ctaactgtcc
      421 ttttaagaaa atactggaat ggtgcaaaat caacggaata ccaaagccga ataaatacaa
      481 taaagcagtt ttatggcggg caaggctaaa tttcttcgtt tttgctcttt tagcatacaa
      541 aaagtaagcg attagaaaga ctaagccatt actcatcaaa atcgctaaaa tccgcttttc
      601 cactaaatcg gtcttaaaat actccatcat tgcaacggta ataactgccg aaaaaatcgc
      661 cgacattaat tgaatcgttg tataagcaag tgcctgtttt tgacactgcc ttacactaag
      721 ctgtacattc aaaaacgact ggaacatcgc cccaatcgct acataagcaa taatttcaga
      781 ctggaaatac cagcaaccca acaggataag caaagttgca caaagagtat ataaataccc
      841 tgtactcaag actaaattta gtgaacgctt accataaaaa tagaaatagc gagcaaccgc
      901 accgtcttgg ctaagcccga taaaaattac aaataaagca gcaaaggttt ggtagtagga
      961 aagctcccca taacctgcca cgccgagttt gcgtgaaaag tagggtaaga gcaagaaagg
     1021 aattgacttt gacgccaatt ccccaattaa ataaatcaca ctgtctttaa ctgctttcat
     1081 agttgcaaat tattctgatt ttttgaccgc ttgcgagaaa gcaacatcat aaaatccttt
     1141 cggttgagta tggtggctgt ctgctaagaa tattttgatt gcattgtaag ccttttccat
     1201 cgcgttgtct tgaaaatatg gatttttcat actcggtaaa cggtgttgga aatcggctga
     1261 aatcaatttt tcgataccat ctatgatagc ctgttcatta ctctctacat caattacgct
     1321 ctcatcccgt actcgccctt gctggcgatt accgatattt agcgtaccaa caccacaact
     1381 cggtgcttca atcaagcctg atgagctatt accaatcaac ccatttgaat atttaatcaa
     1441 cgctaaatac tcttccggtt tgactgaggt gaaaaactta aattgatttt catcggtgta
     1501 agccttaaaa cgtgcataaa tttgctctga attggtgtct gaatttgagc cgataaagac
     1561 gaattggtat ttgtctttaa actgctcaaa agcattgagt aacgcctcaa tttgctccaa
     1621 cacggaaaca tcggttaagg tttccgggtg gaagaccact aaaaaataag gcgatgccgg
     1681 aatccctaat ctctcgacta attctgcttg ggtcggcaac actaaatcca aactattctc
     1741 cgcccctaga gaaccagtat tcaccacatt ttcgggcagc tcgcccattt gaatcacacg
     1801 ctgtctatat tcttcagttg aagttaaatg caatttcgac attttggtaa tacaatggcg
     1861 aataaattca tcgtaattac ctaatgtttg ctcaccaccg tgcagatgaa tcagcggaat
     1921 attatgcatc gccgcagccg ttgccaccgc caacatctcg taacgatcgc ctagcacaat
     1981 caccgcatca taacgatgtt gctggaaatg ttcgccaaat cgatctaaac actctgccat
     2041 tgaatgaata atggtctgat tattttcact gtttaaggta agcggaatac gagctgcaat
     2101 ctcaaagcca tcttgctcaa tgaccgtcac cgtattgcca tattgcggat ccaaatgcat
     2161 cgcagtaaca accagagaga aattcacagt cggatcttgt gctaattttt gtaataaacg
     2221 ctttacaatc ccgtattcgg cacgagatcc ggtaatatag gcaatatttt tcatttagtt
     2281 gagctctttt tccaaaatag cggttaattt ttcataacct tttgcattta aatgtaatcc
     2341 gtcatcgcta tagcttaaat ccaatttgcc atatttatcg ctaaatgcct gatctaagct
     2401 aatccatttc accgctaaat tatctttaag atattgattt aacaagcgaa tatcgttgtt
     2461 attacgatcg actctaaatg cagttggggt aatttccaag aaatagagat taatatctcc
     2521 acgaattgct gttaattgtg caattaaatg attaatatct gctagaattt tttcatcact
     2581 ccagccagca tatacaatat cattagtacc gaacattaaa ataatgtctt taggtaaagc
     2641 agaaattaaa ccttcatcca aaatcagatc actgtattgt tttgttgaaa tacctgcaat
     2701 tcctaaatta ttaactgcct taccgtttaa atgttcaata tcccaatatt caatgatcga
     2761 atgcccgatt aaggctatct ctttcacttc tttaaagttc tcttttttca ttaaaatcga
     2821 acgtttaatg gtttcaagta agattttttc tttattgcgt tgttgcagaa taaagtagaa
     2881 gtactcgaaa tcttggcgat catcaatatc aatagaaacg tctttatcca taaaataggc
     2941 tagacatttc gcaccataga agtgtttttg tttaagatag gcttctggct tagctgtaaa
     3001 aatagctcca ttcggtgaat attcaggatg atgcaattgg cgtgcatagt tagaataatc
     3061 caactgaaaa tgctttaggc actcgtcttc ctcaatcata cgagtcaatg tcgtaggttt
     3121 atgggcatca gaaacagata ctaagaaatc aaattgttcg aaatttttct caaatttttc
     3181 acacgattcc ttaatatgct ctcctgatct caacggcgaa gttggttgaa gtagcataaa
     3241 atagtcaaac tctactgatc tatattgatt taacacatct tcaatcacga caaaagaact
     3301 cgccttatca ctggctaaat gctcggcacg tttaataaat tcaattggat aatgtgagaa
     3361 caaatcaatg tattcttggg aatcagtact aacgataatt ttctcaaatt gccccgactc
     3421 aatcgcagct tcaatagaat aggccattaa cggcttaccg ttggctaata atacattttt
     3481 attcggcaag ccttttgaac cggctcgggc tgtaataatt gcaatttttt tcataaattt
     3541 gaccgcttgt taaacacaaa atgatggagc tttcatccca tcactatatt atattacatc
     3601 tgattttcaa aacgagaatc ttgaattaat tgatcttctt caaaatcctg ttgtgcttct
     3661 ttacctagca catcatacca aaacatcggg ctaatgccat taccggggcg tttagtcgta
     3721 atattttcgg ttgtaaaaat ttcgcctttt ttaattggac gtgccgcaat aattgatttt
     3781 cttgccacaa ttttattttt tgcttccgaa ttagtgacta atttctcgga agaacccagc
     3841 gatttttcca ccgcacgaat gccttcacac agtaatttca gctcttcagg tgtaaccgaa
     3901 gctttatgat ccggaccttc aaagttttta tctaaggtaa aatgtttttc aatgaaggta
     3961 atgccgtaag gtactgccgc caaccctgca aaataaccgg cagagtggtc agaaaaacct
     4021 aaactatagt cgccaaactc ctgttttagc tgatggaacg catttaaatt cacatcttca
     4081 taaggtgtcg ggtactcggt attacaatgt aaaatcgtaa tatctttttt cgctacaccg
     4141 ttatcttcca atacttttaa tgaggctttt gtttcttcaa tggttgccat accggtagaa
     4201 ataacgatcg tcttgccttc aatcggaagt cgtgcaattt tttctaaata cggcaaattg
     4261 gttaattcac cagacggaat tttccacact ttttgttgtt ggcttgccaa gaaatcaatc
     4321 gactcgaaat cgaatggcgt agagaatacc tctaacccca atgaacgagc gtaagcctct
     4381 aatttcacaa actcctcata aggtaactct aatttgcggg tcatttctaa ttgggaatca
     4441 gcggtgccgg tcgttacttt ttgatactcg gctttagggg catatttaga gatcaactta
     4501 tccgctttaa aggtttggaa tttcaccgca tcgacaccac actctttcgc tacatcaacc
     4561 attttctttg ctaacgcagg atcgccgtta tggttacaac cgatttctgc cacaataaat
     4621 acatttacca ttattttata tccttaatat attttgctgg aacgcctgct acaactgtgc
     4681 gaggctccac atttctaatt accactgcac cggagccaac caatgcactt tcacccactc
     4741 ttagctgtcc gttcacaaca gaggaactac caataaaagc atgatcttcg acaattacat
     4801 cgccatttag tgtcgtattg gttgaaatat tgcaatgact gccgataaaa caaccatgct
     4861 caaccagcga tttagtgtta atgatcacat tatcgcctac agttacgcca ctgtttacaa
     4921 ttgccatttt tccaacaaac actccaatgc ctagagtgga atttttggac accaatgcgg
     4981 ttttatct
//
LOCUS       DANLUA010000174         4943 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLUA010000174 DANLUA010000000
VERSION     DANLUA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..581
                     /locus_tag="PXB44_002197"
     CDS             <1..581
                     /locus_tag="PXB44_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL5282635.1"
                     /translation="TTIMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGKK
                     DFFIMNSDTIKQTITSTTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGVR
                     MAIGAKQRNILQQFLIEAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVII
                     AVLCSTLIGVVFGYIPARNAAQLNPITALAQE"
     gene            665..1435
                     /locus_tag="PXB44_002198"
     CDS             665..1435
                     /locus_tag="PXB44_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL5282636.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(1516..2646)
                     /gene="nrdB"
                     /locus_tag="PXB44_002199"
     CDS             complement(1516..2646)
                     /gene="nrdB"
                     /locus_tag="PXB44_002199"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5282637.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(2673..4943)
                     /gene="nrdA"
                     /locus_tag="PXB44_002200"
     CDS             complement(2673..4943)
                     /gene="nrdA"
                     /locus_tag="PXB44_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5282638.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
BASE COUNT         1528 a         1040 c          916 g         1459 t
ORIGIN      
        1 ataccactat tatgcaacgc atttcaggtg caaaacagat agattcactc acggttaaaa
       61 ttaaagatga tattgaaagc caaactgccg aaaaaagcat tgccgaattg ttagtagcta
      121 aacacggcaa aaaagatttc tttattatga atagtgatac gatcaaacaa accattacca
      181 gtaccaccaa tacaatgact ttgctgatct cctctatcgc ccttatttcg ctgattgtcg
      241 gtggtatcgg ggtaatgaat attatgctcg tttccgtcac cgaacgcacc cgagaaattg
      301 gtgtccgaat ggcgattggc gcaaaacaac gcaatatttt gcaacaattt ttaattgagg
      361 cggtggttat ttgcctaata ggtggtgtga ttggcatttt actggcaagc gctattattt
      421 gggcatttaa taccttaggt acgaatttta aaatgatgct ttcaccggaa tcggtgatta
      481 ttgccgtgct ctgctctaca cttatcggtg tggtatttgg ctacattccg gcaagaaacg
      541 ccgcccaatt aaacccaatt actgctctgg cacaagagta aatgcgataa ctatctgcta
      601 cactgtaggg gcaaaaaatt ttttgcccct acatccatta ttatgccaag tttaaggaga
      661 taaaatgaca ataaccaata ctgaactaga acaaattctg aacaccaaac tcaacagctc
      721 cgccattaac gactatgccc caaatggctt gcaagttgaa gggaaaagag agatcaaaaa
      781 aattattacc ggtgtgacag ctacacttcc cctgattgaa aaagcgattg agaaaaacgc
      841 cgatgcaatt ttagtccacc acggttattt ttggaaaagt gaaaacccgt gcattcgagg
      901 aatgaaaggt aaacgcatta aacagctttt agtgaatgac atcaatctct ttggttatca
      961 cctaccactg gatattcact ccgaactagg taacaacgcc cagcttgcta agcatttagg
     1021 agtaaaaaat ctacaaggtt tagaagacag acctaattca attccaatgt acggtgaatt
     1081 agaaaatccg atttccgcca atgaactgaa acaaaaactt gaaaaaacgt tacaacgcac
     1141 cgttatttta tgtgatgaat ttgttcatag cccacaaaaa atgattaaaa caatagggat
     1201 ttgctccggc ggaggacaag gctatattga tttagccttt gaaaaaggct gtgatgcctt
     1261 tattagtggc gaaatttccg aacagaccac tcactctgcc cgtgaacaag gaatctatta
     1321 ttttgcttgc ggtcatcacg ccacggaacg agatggcgta aaagccttag gtgaatggtt
     1381 agccgttgaa tatggtttag atgttgaatt tattgatatt gataaccctg cataaaaaat
     1441 acaagcggtt gaatttcctg aaaattttgc aatttgcaaa aaatcactaa aaatcaaccg
     1501 cttgtatatc atagtctaaa gttcaaaatc gccaaaatca cggctatcta ctttagcatc
     1561 aatctggcca accaaataag aactgacttc cacttcttgt ggtgctacct gtacattatc
     1621 ggaaaccaac cacgcattga tccacggaat ggggttggaa cgtgccttaa atggtaatgg
     1681 tagtccaaca gcctgcatcc gaatattagt gatatattca atatattgca ccaaaatatc
     1741 ccgatttagc cctatcatcg aaccgtcttt aaacaggtat tccgcccacg ctttttcttg
     1801 ttcagctgca cttaaaaaaa gctcgtaggc ctcttggcga cattcacgtg caatttctgc
     1861 catttcagga tcatcattgc cataggccat aatattcaat atatgctgag tgccggtaag
     1921 atgtaatgct tcatcacgtg caataaattt gatgattttg gcatttcctt ccataagctg
     1981 ccgttccgca aaggcaaatg agcaagcaaa agagacataa aaacgaattg cttcaagcgc
     2041 attcacactc atcatacaga gataaagttg tctttttaga ctacgcaaac tgacctcaca
     2101 ggtttttcca tcaacctgat aactgccttc accatacaaa ctatagagtt ggctgtcacg
     2161 aatcaaatca tcataatagg cggaaatatc tttggcacgc ttaataattt cttcgtttgt
     2221 gacaatatca tcaaatacta ttgatggatc attgacaata ttacggatga tatgtgtgta
     2281 agaacgactg tgaattgttt cgctaaatgt ccaagtttca atccaggttt caagctcggg
     2341 gatagacact aatggcaaaa gcgccacatt cgggctacgc ccttgaatgg aatccaacaa
     2401 ggtttggtat tttaaattgc tgataaaaat gtgcttttca tgttcaggca gttgggcata
     2461 gtcaatgcgg tcttgtgaca catccacttc ttccggtcgc cagaaaaatg agagttgttt
     2521 ttcaatcagt ttttcaaaaa tttcgtattt ttgttgatca taacgcgcca cattcacatt
     2581 ttgtccaaaa aacatgggtt cttttagttg atcattttta tgttgtgaaa acgtagtata
     2641 tgccatgtta ttctccttta tttctgttct tattaaattt tacaagcacc accagcacag
     2701 ccttcatcta agtcgtcttg cacatcttct gctccatcac gggtattttg atagtaaagg
     2761 gtttttaacc cgtatttata cgccgttaat agatctttta ataatacttt catcggtact
     2821 ttagcttctg caaagcggtt tgggtcgtaa tttgtattcg ccgaaatcgc ttggtcaaca
     2881 aatttctgca taatcccaac caaatgcaaa tatccgtcca tattaggaat atcccaaagc
     2941 agttcatagt atgcttttaa acgttcatac tccggcacaa cctgctttaa aataccgtct
     3001 tttgatgctt taacactaat atagccacgc ggaggttcaa ttccattggt ggcattcgaa
     3061 atttgcgacg aagtctccga tggcattaag gcagttaaag tggaattgcg caagccaaat
     3121 tgcagaatat cctgacgtag ttgttcccaa tcataatgaa gcggttcttg agttaattca
     3181 tcaatttctt ttttataagt atcgataggg agtaaccctt tggcataagt ggtttgatca
     3241 aaataagcac acgccccttg ttctttggct aaattcatcg atgcttttaa taaataatac
     3301 tgaatcgctt caaaggtacg gtgagttaaa ttattagcac tgccatcgga ataacgcact
     3361 tggtttttag ctaaatagta agcatagtta atcacgccta cacctaatgc gcgacgattt
     3421 aatgagctgt tacgtgcagc taataccgga taatcttgat aatctaatag ggcatctagc
     3481 gcacgaacca ctaaatcagc caattcttct aactcatcta aattgctgag tgtgcctaaa
     3541 ttaaatgccg ataatgtaca taatgcgatt tccccttgtt catcataaaa atgtgccagt
     3601 ggcttagttg gcaaagcaat ttctaagcag aggttcgatt ggcgtatcgg tgcgacacga
     3661 ggattaaatg ggctatgagt attacaatga tcgacattct gaatataaat tcgccctgtt
     3721 gaagcccgct cttgcatcag taatgaaaac aattctacag ctttaacttt acgttgccga
     3781 atgctcggat cttgctcata ttgcaaataa agttgctcaa atttagcttg atcagcaaaa
     3841 aacgcttcat ataaccccgg cacatctgat ggactaaata aactaatctc accgccttta
     3901 attaaacgtt gatacatcat tttattaatc tgtaccccgt aatccatatg gcgagcacgg
     3961 ttttcttcca caccacggtt attttttaat accattaagc tttctgcttc caaatgccaa
     4021 atcggaaaat acacggtagc cgctccaccg cgaacaccac cttgagagca agactttact
     4081 gcagtttgaa aatgtttata aaagggaata cagccggtat gaaatgcttc tccaccacga
     4141 atggggctac ctaatgcccg aattgcgccg gcattaatac cgatcccagc acgctgtgaa
     4201 acatatttaa cgattgcagc cgaggttgca ttgatggaat ctaagctatc atcacactca
     4261 attaacacac aggaactaaa ttgacgagta ggtgtacgca ctcctgccat aatcggtgtt
     4321 ggcaaagaaa ttttgaaagt ggaagtcgca tcataaaaac ggcgaatata atctaagcgg
     4381 cttgctgttg gatacttaga aaataaacag gcagctacga gtaaatacaa aaattgagcc
     4441 gattcataaa tctctccgct aacacgattc tgcactaaat attttccttc caactgcttt
     4501 actgctgcat aggaaaacgt catatcccgc cagtgatcaa taaatccatt cattgtctcc
     4561 cattcttcgg gcgtatagtc gctcagcaag tctgcatcat atttacctag cgcaactaat
     4621 tttttaacgt gctcatataa atgcggtggc tcaaattgtc cataagcttt tttgcgtaag
     4681 tggaaaatcg ccaagcgtgc ggctaaatat tgataatctg gcgtatcctt agtgattaga
     4741 tcagctgcag ccttgataat agtttcatga atatctgaag tgcgaatacc ttcataaaat
     4801 tgaatgtgag aacggagttc tacttgtgaa acagaaacat tctctaaccc ttctgccgcc
     4861 caagtaatca cacgatgaat tttatctaga ttgagttgct cgcgcgtgcc attacgcttt
     4921 gttaccatta aacttttatt cat
//
LOCUS       DANLUA010000175         4890 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLUA010000175 DANLUA010000000
VERSION     DANLUA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..108)
                     /locus_tag="PXB44_002201"
     CDS             complement(<1..108)
                     /locus_tag="PXB44_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4/N-6"
                     /protein_id="HDL5282639.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFSHNIAR"
     gene            complement(249..2735)
                     /gene="torA"
                     /locus_tag="PXB44_002202"
     CDS             complement(249..2735)
                     /gene="torA"
                     /locus_tag="PXB44_002202"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5282640.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(2754..2950)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(2954..4069)
                     /locus_tag="PXB44_002203"
     CDS             complement(2954..4069)
                     /locus_tag="PXB44_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5282641.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4211..>4890)
                     /locus_tag="PXB44_002204"
     CDS             complement(4211..>4890)
                     /locus_tag="PXB44_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282642.1"
                     /translation="KVDIGIESLTLSLREQGWNVDYLELTQEQDNYATLMAQVHNLAP
                     SIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGVLWSTNSGLSE
                     NYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFKAVSNAIRLQP
                     HYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPELFANAQFILP
                     PWFSEKA"
BASE COUNT         1298 a         1090 c         1006 g         1496 t
ORIGIN      
        1 tcgggcaata ttatgcgaga agtccggcaa ctcacgcaat aggcaatatg gggtttgttt
       61 tgcttttttg acggcttctt ctttgccgta ccaagtaagg gttgtcataa aatgcacctg
      121 aaaagtaaaa gtggattatt gtaacaagcg gtcaaaaaaa tagaaagttt tacaaaaaat
      181 taagcaaatt taaccgcttg caacatagaa aaggcacagt tttatactgt gcctttggtt
      241 atatgaaatt agccttgtgt gtatttaggc tctttaaata ctgttacttc tggtgcttgt
      301 ccggtgaatt tttcaacctg taccgtagcg gtatttggtg agttaccctg tgctaatttt
      361 gatgtgcctt catcacgggt tagcacgtta gggttgccgt ttttacatag cggttgctcg
      421 ctggtgccga gatcgagcgg atcgtaccaa gctccttcgt gcaaggctaa tgtacctttg
      481 ataatgccat ctgtcaccac cgcaccggct aatacttgac cacgtttgtt aaaaatacgt
      541 acaatatcac cgcttgtaat gccacgagcg gcggcatctt cagtatggat caacaccggc
      601 tcacggtcat taaccgcata ttgtttgcgt aatgatgagt aagctaactg gctgtgtaaa
      661 cgccatttag agtgtggagt caccaatgcc aacggttctt ctgcggtggt tttacccgag
      721 tattcttcgt gttccatcca agttgggtga cctttacagt catcataatt cattttggca
      781 atcacctcag agaaaatctc aattttgccg gacggtgtgc ctaatggatt taggagtggg
      841 tcattacgaa attcttcata acgaacccat tttttcgctt tgtctgatgc tttaaaagaa
      901 agtggtttat tctcttgcca gaatttctcg aatttcggca taatcacacg atttttgcgt
      961 gctgcctcaa atgcagtttg atagaaactt tgtagccaat ccatttcaga tttgccttcg
     1021 gtaaattccg cttctttgcc tgcacgtttg gctagttcgg caaaaatatc gtagtcgttt
     1081 tttgcctcaa attgcggttc gaccacctgt ttcatcggga aaatattcat cattgaataa
     1141 tcgcccgaca tagttaagtc attacgctca tagctggtgg ttgccggcag cacgatgtca
     1201 gacatacgag ccgttggtgt ccaatggcat tcattgacga taatagtatc cggtttttgc
     1261 cacgctttca ccaaagtgtt ggtgtcttgg tggtgggtaa acggattacc ccctgcccaa
     1321 tagacaattt tgatatcagg ataagtaatt tgtgtgccat tgtaatcaat ggttttgccc
     1381 gggttgagca gtgcatcgga aatacgagcc accgggaatg aagattttga tgtttcatct
     1441 aaccaggttt tcgctccggc ttctactgcc ggatttgctg tgatagagcc taaaatgccc
     1501 cctgtggcac tcggcacgcc gccgtttgag taatggtagc ttaaaccgaa accaccaccc
     1561 ggtaagccga tttgacctaa cattgcagct agggtgacta acatccaatg cccttgttca
     1621 ccgtgtcgtt ggcgttgaat cccccagcct gccattaaca ttgtgcgttt agattggaaa
     1681 tcagtcgcta attttttaat ggtttcttcc ggcacaccac aaatttgact tgcccatttt
     1741 gcatctttcg gttgattgtc tgttttaccg agcagatatt cttcaaactt ctcaaaaccg
     1801 gtggtgtatt ttttaaggaa tgcttgatcg tgtttgcctt cagcgactaa ggtatgagcc
     1861 ataccgagca ttaacgcaac atcagtgccg gtgtttaccg gtacccattc tgcacctaaa
     1921 aattcgcaag attcactttt taccgggtca atacagataa tgcgtttccc gctctctttg
     1981 agttttttga agtattcaat accttgttga tcggtagaag tccacgcaat gcgtagcgta
     2041 tttaacgggt tagctgacca aagcaccaca atatctgaac tttctaaaat cacttcccaa
     2101 ctagtttgct gttcgtacac ctcgattgta cccaagacgt gtggcataat cacttgagca
     2161 gcaccggtag aatagtcgcc ttttacacca acaaatccgc cggtaacatt taaatagcgt
     2221 tgtaacagtg tacgggaagc gtgtaatgcc ccagaactat accaaccgta agaaccacca
     2281 aaaatagcat tcgccccgtg agcctcacga acacgtttca tttcattcgc aactaattca
     2341 aatgccttat cccacgaaac tcgcacccat tcatcacgcc cacgtagagt agtatcttta
     2401 ttaccttcta agtagccttt acgcaccatt gggtatttta ctcgtgtttc accgtgtact
     2461 tggtctgcta ccacgtgttg caattcattt ggcacagaaa tctcaaaaac agaatcagat
     2521 ttcaccactt tgccattttc taccactaca cctaaaggcc cccagtgggc ggcagttact
     2581 actgttttca tctcggctgc tttggcactg gtggaagcta atgcccccac tacaccggta
     2641 gtaccactgg ctaaacctgc accggcagct aagcttaatg atgaattttt tagaaaaccg
     2701 cgacgactta cgtcaatatt gtcttgtttt ttcataataa acctcgtttg aagttaagtt
     2761 aaaaatagac acctttctaa agtcgagtgg ataaaccatt tctcgtgtat ccattggtaa
     2821 aagcaccgac ggtaattttt gcaaaatgtt aggaaaaaat gaccgcttgt agtaggtgct
     2881 ttacggcttg ctaccatatc tctgctctca gcagacactt aggctcacaa gctcggtatc
     2941 ctctaagttg caattaatgg gaactgcctg ctacatcttt tgcattacgt tggagataga
     3001 tagtcaccgc acgcacatca tcagccgaca tagatgttct gtctttcatt gaatttacca
     3061 ccccaatcca ttgatttgcg gtgtaatgtt ctgccccaat cggtgcatgg caaccgctac
     3121 agtgtgtttg gtttaagtta tcgccaaaat gatttagtgc agtaatattg gcagtgactg
     3181 catttttagg agcagtaatt tcaaaattga tttctttcca atttgagttg gtgacattat
     3241 caaacactgt ttttgtgaca ttcactttgc tttgagcgtc atcaggtaga agagcaacca
     3301 taatacgctt gcctaaatcc atatacacca cagattccgc accttcttgt tgccagccat
     3361 taaccgttgc tttaacctgc tcgccttctg ctgtccattg atgtaattct gcataaggta
     3421 acaagcgaat ttctccacct tgagcgagct gcactggagt cattgcgagc gtataaaggg
     3481 ttttatcatt tgtgctaaat tccgcaccgt gtttagctaa ttcgcccatt gctgcggcat
     3541 tatctaccgg cacatcaggc ataaaatggg caattccttt atggcaatca atacaggttt
     3601 gattagtttc ttgggctagt ttgtgcattt tctgtgccgc ttctgtttgt tcagaaagta
     3661 ccatcgcatc aaaactatga cagcttttac aggtcataga atcgttttct ttcatttctg
     3721 cccaaacacg ttgtgccatt tctagacgat gggcttcata ggcttcttga tcaggtaatt
     3781 tattggttac aacggtgtgc caaacatctt ttaatgcgat gattttagct ttgacataat
     3841 gcaagccgtc ttgtggtaga tggcaatccg aacactcggc tcgaattccc ttacggttgg
     3901 aaaaatggac actgccttcc cactctgcct gtgggtgagt catagaatgg cagctcacac
     3961 aaaattctgt tgagctggtg gctttcattg catattgtgt gccacctaat accgcagcac
     4021 ctaatgccat aataccgaca aatacagcaa tttttttcat cttagccata aattacccca
     4081 cttaatacac taaaaattaa agacagaggc attgtattaa ctcccaagag gtataaaaat
     4141 aagtaaaaat acctattacc tcgaaagagt gagaataatt tgatctaagg caaaaatatg
     4201 tagtgaaatt ttaggctttt tcagagaacc acggcggtaa gataaattgt gcattggcaa
     4261 acaactccgg tgcaatcaca cggctctgcc cttgttggat ttggtaaacc aactgtggca
     4321 agctgataga taagtcgtgg gtttctgcgt aagttaatcc aatttggcta tcggtattaa
     4381 aatagtaagt gccgtttacc ccataatgtg gctgtaagcg gatcgcattg gagacggctt
     4441 taaaatgccg tggtgaaaca ctctgtttcc acgcatttgc cagaatattc gcttggtcat
     4501 aagcaatact ggcttgggaa taagtgggcg atttgccgaa aaaccgttcg taatgttggc
     4561 taaactgtct gcctaaataa ttttcgctta acccactatt ggtactccaa agaacgcctt
     4621 cgcaaagttt ctcgtgtggc aaaaacgccg aaggtgcata aatactgtaa ataatggcat
     4681 ttgttgggtt gcgaatgaac gtttgataaa actgtacaat atcttctgca aaataagagg
     4741 caaacacaat aatggaaggt gcgagattat gcacctgtgc cattaatgtg gcataattat
     4801 cttgctcttg ggtgagttcc agataatcca cattccagcc ttgctcccgc aaacttaggg
     4861 ttaaactttc aataccgata tcaacttttt
//
LOCUS       DANLUA010000176         4834 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLUA010000176 DANLUA010000000
VERSION     DANLUA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(40..606)
                     /locus_tag="PXB44_002205"
     CDS             complement(40..606)
                     /locus_tag="PXB44_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282643.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLVN"
     gene            complement(1139..1465)
                     /locus_tag="PXB44_002206"
     CDS             complement(1139..1465)
                     /locus_tag="PXB44_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282644.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1513..3144
                     /locus_tag="PXB44_002207"
     CDS             1513..3144
                     /locus_tag="PXB44_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL5282645.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3059..3952
                     /locus_tag="PXB44_002208"
     CDS             3059..3952
                     /locus_tag="PXB44_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5282646.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3945..4808
                     /locus_tag="PXB44_002209"
     CDS             3945..4808
                     /locus_tag="PXB44_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5282647.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSEI
                     YLTLKEVCDIQ"
BASE COUNT         1589 a          926 c          936 g         1383 t
ORIGIN      
        1 gtcggcaata acattatttt ataataacta atgtgaattc tagttcacta aatcgaattt
       61 cttttggtaa atctcattcc tttctaatat atagcgtttt acctcatcat cgtaaaacca
      121 ttcaagaaag ttatcaacat cttcaatgaa cttttctacc tgttgctgtg catatgctat
      181 cgtatcggta gggaagattt gataatgaaa cggattgcgt tcatcataac tctgccattt
      241 gtcataatcg gcttgtttac gccgcgcttc tcttgcctcc cagtcgattt tttcttccac
      301 ttgatattct ggctctggct tattatacaa aatacggatg agttcctttt cttcatcctc
      361 aagcagaata tcttcaaacc agatttcaga aaataggctg cgcttgaaaa tgaaagactt
      421 catcatttcc cctccacacg ccaataagtt tcccaatgct tcccgtttcg ctctatcttt
      481 tgccgaaaat atccagtttt tcgatacaaa acagtacatt ggcatgtcat gcaaataggt
      541 tcttcgtgaa atggctaatt caggaaaatg aggaataagc tgtaacccca ttattaagcc
      601 ttccatactg cacacgatct tacttaattg tccgtaatcg cgtaaaaaag caatgtcagt
      661 ttttatcaac cacaacgata ggctgactaa tatgaacata aagatgagat aatagatcgg
      721 ttttaaggct aaggcgcgcc gacaaagcca cattaagagt ggaatcgtaa cgagatcaaa
      781 gaaaaatgat gagtctatga tgatttcctt atgtgagttg atgaataatc aggcatgaag
      841 gaaaccttaa aaaggaaaag tcccttcatt accacttttt aaaacctttt aaggcttaaa
      901 aatcaaaagt cgtaatgaag ggactattgt ccgtgggcaa aaaggtatta gttatagggg
      961 gctttgtcaa gcgaaaattt actgtgcatt ttctcgccaa aactgattta agccttgata
     1021 aaatctcgaa agtaccttgt caagaaaaat ctttttagtg gtttgattgc tagttttttg
     1081 agcaaaacta tgctgtaatg cagtaaaaat acagtttcta tctgtgtttg gaatccagtt
     1141 acaaattgga gagtaataca aagcatattt gcttgagcat ctcagcatct tggtagcatt
     1201 cttctgattt tgaaatatcc caaacaagct gccgtacaca tgatgaaatt ctcattaacg
     1261 ccaataaatc taggctactt ggcaatcttt tccctgcaac ccaatcttta actgttttct
     1321 cactcacatc taatgcattg gcgaccattt tccccgtcca atattgagca tgcatttcct
     1381 ttcgtaacgc aattgcaaac cgctctttcg tttcaatctc attgagtgta attttgggta
     1441 agattttacc ctttgagtgc aacatatttc ctcccgattc ctttatattg gtgtagataa
     1501 ggagtcatta gaatggttac acgtacaaag aatcaaccta aaaatcaggc ggtttcctac
     1561 attcgaatgt caacggaaca ccaagaattt tcaccagacc ttcaacgttg ctttatcgaa
     1621 aattatgcta aagaaaatgg gctagaaatc attagagaat acattgatga aggaaaaagt
     1681 gggctaaccg cagaacaccg cccacaattc ctttcaatga tacaactcgt acaaagtgga
     1741 caagccgact tcgatcatat tttggtgtat gacgtgagtc gttggggtcg gtttatgaat
     1801 attgatgaat cggggcatta tgaactgatt tgtacctacc aaggcattaa agtccactat
     1861 tgcgccgagc cattcaaagg gaatgatatt gcctctcaaa tatataaaac catgaaacgt
     1921 ttgtcggtag gagactactg tcgtgaactc ggagtgaaag tatttaacgg tcaaaaaaat
     1981 ctgatccaac atggatttag acaaggtggg gtagctggat ttggattgcg taggagattg
     2041 attgactgtc aaggaaaccc taaatttgat ctccaacgtg gagatagaaa gagcctacaa
     2101 accgacagag tgattttggt tgcaggccca aaggaagagc aggaaattgt ccgacaaatt
     2161 taccatgatt ttgtttatca acataaaacg gaacaacaaa ttgctgatag cttaaatgca
     2221 caaggcattg ttactgatag aaatacagca tggacgaaag gcgtagtaca tcaaatcctt
     2281 attaacgaaa agtatgtcgg ccacaatgtt tggaataaac gtagtgcatc aaaaacacgc
     2341 gaatataaag gtaaaaaccc cgtagaagag tgggtgcgag ctgagaatgt atttgaagct
     2401 atcgtgccac aagccttatt taatgcagcc caaaccatta ttcatcaacg ctccgcccga
     2461 ttatctgacg atgaaatgtt agagcaactc aagctacttt tgaaagagaa agggaagctg
     2521 tctggcttga tcattgatga agccgaaaat tgtccatcaa gtagcgcgta cagtagccga
     2581 tttggaagcc tacttaacac atatacttta attaactatg aacccgaacg ggattaccac
     2641 tatgtagaga tcaaccgatt actccgtcag cagcataaaa atttcgtaca acaaacactt
     2701 gataccatta ttgaattggg gggagtggtt ttgagcgatg atgtaaccga tttagtccac
     2761 atcaataatg aatttaccgc ctcaatcgta ttaagtcgtt gtcgcctaac gccatcagga
     2821 agcaaaagat ggttgatccg atttgatacc agtttaaatc ccgacataac aattgcagtg
     2881 cgcatcaatg agagtgccac agaaattctt gattactacc tgcttcccac aactgaaaag
     2941 gtaaatgaaa aactacgatt ggctgagagt aatcctgccg aacttgaaat ctaccgccat
     3001 gataatttag atcgcttttt tattatggtt gaacgtattt tagtcaagga gtttatctat
     3061 gcaaaacgac accattcaaa ttcttcctat cgacaaaatt cgggtactca atcccagaac
     3121 gcgcaatcag aaagtctttg ctgatctggt cgaaaacatt gcaacgattg gtctcaagag
     3181 acctattacc gtaacgccat ctgtcgatag tgctgaatat tatgatttgt tatgcggtca
     3241 aggtcggctt gaggcttgtc aggtgcttgg tgagctattc ataccttgtc gcgttgtgaa
     3301 cgcaaatagt gaagaaagct acttgatcag ccttgtggaa aatattgcca gacgtcagca
     3361 ttcctatatg gagctgcttt caggcatcaa aatactcaat gatagaggtt ataagcccgt
     3421 tgatattgct cgcaaaatcg gtttctcaaa aagccatgtc aacagtatct tacacctcat
     3481 caatgctggg gaagagcgcc ttatcaatgc ggtagagcaa ggaattttac ccattaccat
     3541 cgctatttcg atttctcgtt cagacgatca ggaagttcaa aaacaactcg cggaacttta
     3601 caccaatggt acattgaaaa gcaccgatat tgccaaaata cgtaacctga tccaccgtag
     3661 aaatcttgct ggtaagaaag ccaatacagg tatttctgga agtattagat catctattta
     3721 taatcccaaa actgtggtga atatctacaa agaggaaacg gaaagacaga aaatgatgat
     3781 caagcaagcg gactatgatg aaaaacaact ctctattatc ttgtcttgct taaataaact
     3841 ctttgaggac aaatactttc aactcgtcct taaatcagaa catttagacg atatgccaaa
     3901 agatttaagc caacgaatgt taaaacaacg agagggaaat atttatgcct aaacatatta
     3961 cttatgcctt tcataatcag ttaatttcgc ttccaattga tgagattatt ccattaaaaa
     4021 agatggataa aaattttgag aaaagcacta agttccaaag cattctggct tcaattcgtg
     4081 aaatcgggat tgttgaacct gtggtcgtct atccagaaga aaacaaccaa tatattttgt
     4141 tagatggcca ttctcgtgta catgcactca aaaagctagg tgtggataag gtgctttgta
     4201 tgatttctac cgatgatgaa ggctttactt acaacaagca aattaaccgc ttaaccgccg
     4261 ttcaggaaaa taaaatgctg atgaaaacca ttgagcgcgg ggtttcggat gaactgatcg
     4321 caagaacact caatatcaat ctaaaagcac tacggcagaa aatgaatatg cttaatggga
     4381 tcacaagaga ggttgttgat atattggcca ataagcaggt cagtaaagag atatttcgga
     4441 ttttgcgcaa gatgaagcct gaacgccaaa ttgaagtggc acaaatgatg atcgccagta
     4501 ataagtttac attgacatac gccaatatga tgttgctcag ttcacgcaag gaggaattag
     4561 tcgaaagcca caagtctaaa accacgaatg atgatttaac cgatctttcc attatgcaaa
     4621 aagagctaga gcgacttaaa gagaactata aaatttccga agagaaattg gcggacttaa
     4681 aaatgggatt ggttgtggcg aaagggtatg tcaaaaaact attgaataat gctgccgtgg
     4741 tagatttgct tgaaaatgat cagagtgaaa tctatcttac tcttaaagaa gtatgtgata
     4801 ttcaataata taatatctag ctaataacct taat
//
LOCUS       DANLUA010000177         4824 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLUA010000177 DANLUA010000000
VERSION     DANLUA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1129)
                     /gene="dnaJ"
                     /locus_tag="PXB44_002210"
     CDS             complement(<1..1129)
                     /gene="dnaJ"
                     /locus_tag="PXB44_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL5282648.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDN"
     gene            complement(1352..1654)
                     /locus_tag="PXB44_002211"
     CDS             complement(1352..1654)
                     /locus_tag="PXB44_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282649.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            complement(1796..3694)
                     /gene="dnaK"
                     /locus_tag="PXB44_002212"
     CDS             complement(1796..3694)
                     /gene="dnaK"
                     /locus_tag="PXB44_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL5282650.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3953..4291
                     /locus_tag="PXB44_002213"
     CDS             3953..4291
                     /locus_tag="PXB44_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5282651.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
BASE COUNT         1216 a         1086 c          959 g         1563 t
ORIGIN      
        1 agttatcaaa gaatttcttc acaccatcaa agaaaccttc gtgtttcgga cgatgtttac
       61 cttgtccttc taaactctct tcaaattgtc gtaatagtgc tttctgttct tcggttaaat
      121 tcacaggcgt ttcgaccacc actttgcaga ttaaatcccc tgcgtagcca ccacgagggc
      181 ttgttacccc tttgcctcgt acacggaata gtttaccggt ttgagtttct gccggaattt
      241 tgagttttaa tttgccgtct aaagttggca cttcaatttc accgcctaaa gctgcaaggg
      301 taaagctaat cggcacttca cagtataaat tcgaaccgtc acgctcaaaa atatcgtgtt
      361 ctttaacgtg aatcaccaca tataaatcgc ctgccggtgc gccattttcg cctgcagctc
      421 cctctctcga taagcgaagt tgattaccgg tatccacacc tgccggaatg gtaacagaga
      481 ggtttttcgc tttctgtaca cgtccatcgc cgtggcaaga acggcacggt ttttcaatgc
      541 gtttacctgt accgtgacaa cttggacaaa ctgcctcagt cacaaagaaa ccttgctgac
      601 ggcgaactct gccggagccg tgacaatgcg aacaggtttc cactttgctc cctgcttccg
      661 cccctgtacc atgacaagta tcacattcag ctaaggtttg aatgcgaata tcttttttac
      721 agcctttcac agcttcttca agggtgatct cgatgtcgta acgtaaatca tcgccacgta
      781 ccacacgttg gcgacgtcca ccgccaccaa agccaccgcc aaacatttcg ctgaagatgt
      841 cttcaaaacc accgaaacca ccgccgccaa agccaccgaa tccgcctgca ccggcaccgc
      901 cttgttcaaa ggcggcgtga ccgtattggt cgtacatcgc ttttttctcg ctgtcggtca
      961 gcacgtcata ggcttcggta atctctttaa atttctcttc cgactcttta ctgccttggt
     1021 ttttatccgg gtgatgcttt gccgccaaac gtttatatgc tcgtttgatg tctttctcgc
     1081 ttgccccttt agacagtcca aggacttcgt aataatcttt ctttgccatt gttgttccgt
     1141 ttgtaaattt ttctgtgttt ttgaccgctt gccctcgccc cttgtgggag aggggtagga
     1201 tggttaattc cccctctctc tgaaaaatat ttctgaacga aatatttttc ggtctctctc
     1261 ccacaagggg agagagtttt atataattaa atgggatcta gccaagatgg ctcaagatga
     1321 cacaagcgtg gacgcttgcg ctatcaaaag cctaacaatg cggattcacc gcaatataaa
     1381 tttctacttt gccgtctgct aaatgttttt caaaatcttt ggtgtaagct cgtttgagca
     1441 aaccttgttt ggaacggttt tcaatcacag accaagtgtc tgccaaaaga gcttcggtag
     1501 ttggaatttg ctcaaaccaa acaaaatcgt ccattaaaat cgcggtattg ctgttttccg
     1561 tttctgtggc aatgaccgtt tcgccacttt tatgatcgta tattttataa aacggtggct
     1621 gtacattttc cgttgtaggg tagagtttta gcatttgtaa aatttccttt gaaaagcacc
     1681 gcttgcacag ccccaaaggg gctgaattat gtgtaaccta gcacggctcg tgctaggtgg
     1741 tatatatcta cacaaaaggg gcgaaagata tttcgcccct acaagttacg caagattatt
     1801 tattatcttt cacttcttca aattccgcat cgaccacgcc gtcatctttt tgagattgtt
     1861 gtggttgctc gccgccttgt gcttttgctt gtgctgcttg cattaacggt tctgaagctt
     1921 tgattaacgc ttcgattttc gcttcgatct cggctttatc ttcgccttta gctgcggttt
     1981 ctaactcaga aatagccgct tcgatcttcg ctttatcgtc tgcgtttaac gcatcgcctg
     2041 cttcggtaat ttgcttacgg gttgcgtgag caataccgtc tgcttggtta cgagcttgaa
     2101 ctaactcttc aaattttttg tctgcttccg cattcgcttc tgcatcacgc accatttgtt
     2161 caacttcagc atcgcttaag cctgaagatg cttgaatttt gatttgttgc tctttgccag
     2221 tgtttttatc ttttgcagag acattgataa taccgttagc atcaatatca aaggttactt
     2281 caatttgtgg cataccgcgt ggagcagggt taataccttc aaggttgaat tgacctaaag
     2341 atttattatc cgctgcacgt ttacgctcac cttgtaacac gtggatagtt accgcagatt
     2401 ggttatcttc cgctgttgag aacacttgtg atttcttggt tggaatcgta gtgtttttct
     2461 caattaaggt agtcattaca ccacccattg tttcgatacc aagtgaaagc ggagtaacgt
     2521 ctaataacaa tacgtctgtt accgaaccac ttaacacacc gccttgaacc gccgcaccga
     2581 ttgccacagc ttcgtccggg ttaacgtctt tacgcggttc tttgccaaag aagtctgcca
     2641 cttttttctg tactaacggc atacgggttt gaccaccgac taaaatcaca tcgttgatgt
     2701 cgcctactga taaaccggca tcagctaaag cggtttttaa cggctctaac gaacggttta
     2761 ctaaatcttc aactaaagat tccaatttcg cacgggttac tttaatgttt aagtgtttcg
     2821 ggccggttgc atctgccgtg atgtatggaa ggttcacttc agtttcttgt gcagaagaaa
     2881 gttcgatttt cgctttttcc gctgcttctt tcacacgttg cattgccatt gggtcgttgc
     2941 gtaaatcaac gccttgttct ttttggaatt cttccactaa gtagttgatt acacggttat
     3001 cgaagtcttc accacctagg tgagtatcgc cgttggttgc acgcacttca aaagtttgct
     3061 cgccgtcaaa gttatcaatt tcgataatcg aaatatcgaa tgtaccacca cctaagtcgt
     3121 aaacagcaat cgtttggttc tctttcttag aatctaaacc ataagcaagc gctgccgctg
     3181 ttggttcgtt gatgatacgt tttacatcta aacctgcaat acgaccggca tctttagttg
     3241 cctgacgttg tgcatcgtta aagtatgcag gcacagtaat aaccgcttcg gttaccggtt
     3301 cgcccaagaa atcttctgcg gttttcttca tttttttcaa gatttcagca gagatttgcg
     3361 gcggtgctaa tttatcgcct tttacggtta cccacgcatc gccgttatct gctttgctga
     3421 tttcaaacgg cataatttca atatcacgtt gcacttcagc atcagtaaaa cgacggccga
     3481 ttaaacgctt gattgcaaat aaagtatttt tcgggttagt aattgcctga cgttttgccg
     3541 gctgaccaac taaggtttct ttatcggtgt aagcgatgat agatggcgtt gttcttgccc
     3601 cttccgcatt ttcaattaca cgaggtttgt cgccgtccat taccgcaaca caagaatttg
     3661 ttgtacctaa gtcaatacca atgattttac ccatttttat gattcctata ataataaatt
     3721 cagtttgaat aagaaacggg attgcatttc ccgttcagac gaaatcataa atagggacga
     3781 aaatgcactg ttcaagggca aaaataaaaa aattgcaaaa tttttggaaa aagtaaccgc
     3841 ttgtatgtgg gggagatttg gcagaatatt tcacggagaa aatacctcct accttgcata
     3901 cccctcaata gctttttata cttacggcaa ctcaaacaga gaaggatata atatgaaact
     3961 gattactacc attatccaac cttcaaaatt agaccaagtg caagatgcac tttcagaaat
     4021 cggtattcaa ggaatgacta ttaccgaagt aaaaggcttt ggacgccaat atgggcatac
     4081 cgaacaagag cttagccaaa tgagccgcat tgactttcgt cctaaaatta aggtggagat
     4141 attggtttct aattaccaaa ccgatgaagt gatcgatgcc attctcaatg catcctattc
     4201 tgggcaagtg ggtgatggca aaatttatgt agcgaatatt gaacaagtta tccgcacccg
     4261 taccggcgaa actgacgatg aagcagtcta gccattgcta gcagagaagc tttttattat
     4321 cgtgttttag agttatattt tcaaccttac taaagcaaat tttctaattt ggtgtgaatt
     4381 aaatgtttgt tttttgtgta atacgggtta ttatagtaat caaaaatagt taggttttaa
     4441 tggaaataga cattctttgt tatgctaaat aaacacatta ataaaattat caattcttca
     4501 ttttctcttg cattttttac attaactagt tttgctaccg cttactgtta cggttgggga
     4561 caagccatat ttcatggcta cccttggtgg catgttgaaa tcggcaatgc cagtatggca
     4621 agaagtttgg cttatgtatt cggtacttcc gtcatcttat tcttgtgtta tgcactaggt
     4681 tatagtttac taaataaagt ttttcagctg cattatttcc gaaatctagg ttggttaagg
     4741 gtgattgtgt tggtttctgt gtttacagtg cctgttattc ttacttttta cttatttacc
     4801 gggatagtgc ccatttatat ctct
//
LOCUS       DANLUA010000178         4821 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLUA010000178 DANLUA010000000
VERSION     DANLUA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..446
                     /locus_tag="PXB44_002214"
     CDS             <1..446
                     /locus_tag="PXB44_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL5282652.1"
                     /translation="TVNAAPVELHQFDSPKQESDYRALIQELRCPQCQNNNIADSNAT
                     IATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPATIMLWLLPLILIALG
                     LGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEEK"
     gene            443..1354
                     /gene="ccmI"
                     /locus_tag="PXB44_002215"
     CDS             443..1354
                     /gene="ccmI"
                     /locus_tag="PXB44_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL5282653.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1351..1956
                     /gene="mog"
                     /locus_tag="PXB44_002216"
     CDS             1351..1956
                     /gene="mog"
                     /locus_tag="PXB44_002216"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL5282654.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            1966..2304
                     /gene="glnB"
                     /locus_tag="PXB44_002217"
     CDS             1966..2304
                     /gene="glnB"
                     /locus_tag="PXB44_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL5282655.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2385..2864
                     /gene="lspA"
                     /locus_tag="PXB44_002218"
     CDS             2385..2864
                     /gene="lspA"
                     /locus_tag="PXB44_002218"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL5282656.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2875..3819
                     /gene="ispH"
                     /locus_tag="PXB44_002219"
                     /gene_synonym="lytB"
     CDS             2875..3819
                     /gene="ispH"
                     /locus_tag="PXB44_002219"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL5282657.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3819..4067
                     /locus_tag="PXB44_002220"
     CDS             3819..4067
                     /locus_tag="PXB44_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL5282658.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4274..>4821)
                     /locus_tag="PXB44_002221"
     CDS             complement(4274..>4821)
                     /locus_tag="PXB44_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5282659.1"
                     /translation="HSNHSNHSNQSANQYIFNSDWFTHNVPSLASIFEYVKPRRILEI
                     GSFEGRSSVFFIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQ
                     KFQNVVLYKHRGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKG
                     GVISFDDYLWSPDETGKQRHY"
BASE COUNT         1512 a          970 c         1052 g         1287 t
ORIGIN      
        1 taacagttaa tgctgctccg gtagaactgc accaattcga cagcccgaaa caagagtcgg
       61 actatcgtgc cttaattcaa gaactgcgtt gcccgcagtg tcaaaataat aatatcgccg
      121 attccaatgc gactattgcc accgatatgc gggcaaaaac cttagaattg ctcaaacaag
      181 gcaaaagcaa agacgaagtg gtggattata tgattgaacg ctacggcaat ttcgtcactt
      241 acgacccgcc tctcacaccg gcaaccatta tgttatggct gttgccgtta attttaattg
      301 ccttagggct gggggtggtg ttgcgtagaa agaaaaacaa accacaagcg gtcaattttg
      361 agcaaaatac tgcaaaaccg accactcatc tcgctcaaga tgagcaagaa cgtcttaacc
      421 aaattctcaa taaggaagaa aaatgaattt ctggctggca attattttcg ttacactgat
      481 tgtatgtggc gtggcgtttt atccgttact taaaaagaac aagcaaatcg ataacaccaa
      541 gcgagacagc ttaaacaaag ccttctattt tgaccgctta aaagaagtgg aaaacgaagc
      601 gaatgaaggg gtgattgatg accctgaaca aaccaaatta gagctacaaa aaaacttgct
      661 tgatgatatt ccacacagca cacaaagtgc cacaagtgaa aaattaacgc tcggcaagtc
      721 gtggtttgtg gcattgattt tatttgtcgg cggtatcagc tttgcgattt acagcaatgt
      781 cggttcttgg ttctccaact caatgttaga caccactcac caaaaattag acagcttcta
      841 tgagcgttta aaaaccgaag agaccaatcc gctctcacaa gaggaaatga accaatttgc
      901 gatggcatta cgggtagatt tacaaagtaa tccgaatgac gacaaaaaat ggtatatgct
      961 cggtcaaatc gggatggcgt tagatgatgg tcagcttgca cacgatgcct ttagcaaagc
     1021 ggctcaactc aagccggata acttagatta caaactcagc cacgcccaac tgctgctctt
     1081 ttccgaagca caggaagaca aaaaacaggg tgaaaccttg ttaaaagaga ttatccgcca
     1141 agaccacaca aactttaatg cgttaagttt attggcgttc cactcctttg aaaaagaaga
     1201 tttcaaaatg gcggcaatga cgtggggaat gatgttaaaa ctactgcctg atgaagatcc
     1261 tagcaaagta acggtagaac gcagttttaa tatggcgtta tccaatttaa gtgaggcgga
     1321 aaaacaagaa ttgcaaaaag gtgagaaaaa atgaccgctt gtaaaccact tagcaaagag
     1381 aagttgaaaa tcggcttggt gtcggtttca gaccgagcct cgcagggggt ttattcagac
     1441 caaggcattc ccgaattaag ggcgtggctt gaatctgcct taaccgagcc gtttgagctg
     1501 gaaacccgct taattcccga tgagcaagcc attattgagc aaaccttaat cgagttagtc
     1561 gatgctcacg cttgccattt ggtattaacc accggcggca ccggcccggc aaaacgtgat
     1621 gtcaccccag atgccaccct tgctgtcgcc caccgagaaa tgcccggctt tggcgaacag
     1681 atgcgacaag tgagccttca cttcgtgcca accgccattc tctctcgcca agtgggagta
     1741 attcgccacg acagtctgat tttaaatctg ccggggcaac cgaaagcaat taaagaaacg
     1801 ctggaaggtg tgaaagatga acagggcaaa gtattggtta aaggtatttt tagtgccgtg
     1861 ccatactgtt tacaactttt aagcgaaatt tatgtcgaaa cccacgcagc tatttgcgaa
     1921 agtttcagac ccaaatcggc aagacgagaa aattaaggct aaaaaatgaa aaaaatagaa
     1981 gcaattatta aaccttttaa attagacgat gtgcgtgaag ccttaaccga tgtgggtgtc
     2041 agcggaatga gtattaccga aatcaaaggg tttggcagac aaaaaggaca taccgagctt
     2101 tatcgtgggg cggaatatgc gattgatttt ctaccgaaag tgaaagtcga aatcgtgatt
     2161 cccgacgagt tggaagaaca gtgcattgaa gcaattatgg aaaccgccca aaccggcaaa
     2221 atcggcgatg gcaagatttt tgtgtatgat gtcgggcggg tgatccgcat tcgtaccggc
     2281 gaagaaaatg aagaagcgat ttagttttgc aaaaaatccg gcaaaaacga ccgcttgcag
     2341 ggggatttcc atctactctt tttacattaa attttggata ttaaatgaaa acaggcttaa
     2401 aatttctttg ggtgagttta attgtaatca tcattgacct ttggtcaaaa tacattgtag
     2461 tgcaacattt tgaatttggt gaaagcatta atattttgcc gatttttaac ttcacttacg
     2521 cccgaaacta cggggctgcc tttagtttcc ttgccgacca ttccggctgg caaaaatatt
     2581 tctttttagg gctggcggta gtgatttcca gtgctttagt ggtggctctt tggaagaatc
     2641 aagcggtcaa aaaattagaa aatattgcgt atgccttaat tattggtgga gcgattggga
     2701 atgccattga ccgtgcttac aacggctatg tagttgattt ccttcatttt tattgggata
     2761 tttatcacta ccctgtgttt aatattgccg atattgcgat ttgtgtgggt gcaggcttac
     2821 tgattttaga agcctttata accaaaaaag aaccgaaaaa ataagagtga ttaaatgaaa
     2881 attttattag caaacccacg cggtttttgt gccggcgtgg atcgagccat ttcgattgtt
     2941 gaattagcct tagaaattca cggtgcacct atctatgttc gccacgaagt ggtacataac
     3001 aaatttgtgg tggatggctt gaaagccaaa ggggctattt ttgttgagga gctacacgaa
     3061 gtgccggacg atgctatcgt gatcttctca gcccacgggg tgtcgcaagt agttcgccaa
     3121 gaagccaaaa atcgtggctt aaaagtgttt gatgccactt gcccactcgt gaccaaagtg
     3181 catatgcagg ttgcccgagc cagtaaaaaa gggacaaaag caattttaat cggtcacgaa
     3241 ggtcaccctg aagttatcgg cacaatggga caatacaata accaagaagg tggcatttat
     3301 ttggtagaat cggtggaaga tattgccaac ttgccggttt ccaacaacga tgaattaact
     3361 tttatgacgc aaaccacact ttccattgac gataccattg gcgtgattga ggcgttaaaa
     3421 gagaaatatc cggcaattca aggccctcgc aaaaatgata tttgttatgc taccaccaat
     3481 cgccaacacg cagtgcggga actggcagaa caatcacaat tagtgttagt ggtaggttca
     3541 aaaaactcct cgaactcaaa tcgtttggcg gaactcgcct ctcgaatggg tgtgatgtca
     3601 aaactgattg atggtccgca agatattgat ccaacttggt tggagggtat tgaaactatc
     3661 ggtattaccg ccggtgcttc cgctcctgaa gtgttggtgc aatcagtaat ttcgcattta
     3721 aaaacccttg gcgtaaccga agttgaaacc ttagtcggca acgaagaaga cgtggtgttt
     3781 gaagtcccga aagagttacg catcaaagag gtaaaataat gacagaaatc aaccgcttta
     3841 aattagaatg ggcgtgccga cgtggtatgc gtgagttaga caaaatgatt atgccgtttt
     3901 accaaaatca ttttaatgaa ctcaatgaaa cccaacagca agctttcgtt gaaatgctca
     3961 attacagcga cccggaactg tttcgctggg taatgctgca agcacctgcc ccaaccgaag
     4021 aaatcgctga attgattagg ttaatgcgag ataaaattga gacctagagc taatagatta
     4081 acctcttctt tggggtaaat atatagctac ttaccccaaa gaagactaaa ttcattcaac
     4141 ttttacaaaa taaccctaaa ctagaaaagt taatcttcca atttaataat atgttgctga
     4201 taagctatta aaccttgtat tacatgcact ttctgctgat aggtattgac atagtgatca
     4261 acagcgggtt ttactaataa tgacgctgtt tacctgtctc atctggtgac caaaggtaat
     4321 catcaaaact aataacgcct ccttttctca ctaaacgatg tgcaagtaaa gcatcgaaca
     4381 acacatctgg cgcttcgtga gatccatcta tgtaaatata gtcaaaataa tttatatatc
     4441 catttgcgag aagttcaatc attttttggt gagaatagcc acggtgctta tataacacaa
     4501 cattttggaa tttctgttgc gcaagagtga cattatggat aaatcgttgt tctatttctc
     4561 ccatgtccca aactccttga tgctctgctc ccccttccca actatcaata caatgaattt
     4621 caacttgtga ttgatatttc aatgcttctt caatgaaaaa gacagatgat ctaccttcaa
     4681 atgatccaat ttctaaaatc ctccgaggtt ttacatattc aaaaatagaa gctaatgaag
     4741 gaacattatg agtaaaccaa tcagagttaa aaatatattg gttggctgac tggttggaat
     4801 ggttggaatg gttggaatgg t
//
LOCUS       DANLUA010000179         4788 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLUA010000179 DANLUA010000000
VERSION     DANLUA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(13..88)
                     /locus_tag="PXB44_002222"
     tRNA            complement(13..88)
                     /locus_tag="PXB44_002222"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Ile,seq:cat)
     gene            complement(182..919)
                     /gene="pgeF"
                     /locus_tag="PXB44_002223"
     CDS             complement(182..919)
                     /gene="pgeF"
                     /locus_tag="PXB44_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL5282660.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(951..1337)
                     /locus_tag="PXB44_002224"
     CDS             complement(951..1337)
                     /locus_tag="PXB44_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5282661.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            1418..2419
                     /gene="yejK"
                     /locus_tag="PXB44_002225"
     CDS             1418..2419
                     /gene="yejK"
                     /locus_tag="PXB44_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL5282662.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLLWDELNDTLTIKGLPANLRDQLERNK"
     gene            2486..2827
                     /gene="bamE"
                     /locus_tag="PXB44_002226"
     CDS             2486..2827
                     /gene="bamE"
                     /locus_tag="PXB44_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL5282663.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            3020..4360
                     /gene="gdhA"
                     /locus_tag="PXB44_002227"
     CDS             3020..4360
                     /gene="gdhA"
                     /locus_tag="PXB44_002227"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL5282664.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            4522..>4788
                     /locus_tag="PXB44_002228"
     CDS             4522..>4788
                     /locus_tag="PXB44_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5282665.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQN"
BASE COUNT         1451 a          865 c         1063 g         1409 t
ORIGIN      
        1 gcaacgctta gatggtcccc ccagccggac ttgaaccagc gaccaagcga ttatgagtcg
       61 cctgctctaa ccactgagct atggggggat ttgaaaagtg gttggaatta tagagaaaaa
      121 ccgagtagtc gtctagaggc agaagattac tcggttaatt attgaacgag agaaaatagg
      181 gttattctgt tcgccaaatt aaggttgcca ttcgcccggt ttgtttgtct cggcggtagg
      241 aaaaaaacag ttctggttgg ctgtaggtgc aatattcacc gcctgaaatg gcggtaatgc
      301 ctaatttatt cagacgttgg gtggcaattt ggtaaagatt tcctaaaaat ttaccgcttg
      361 tagtgctgtc ttcaataagg gcttctttcg ctcttgggtc aaaagcacag aattggtctg
      421 ccacttcact cccgacttga aaagcattcg gtccgatagc cggccctagc caagctgata
      481 tttcgtgtgg cgggcagttg aatttctcta ccgtcgcctc taatatgcca tcgcataacc
      541 cacgccagcc ggcgtgtgcc gccgcaattt ccgacccgtc ttggctggca aataataccg
      601 gcaaacaatc tgcagtcatc actaagcaga cttggtttgg ttgattggtg taaatcgcat
      661 cggctttaat attatcacct gaatagggaa gttcaatcac tcgggtgctg tgagtttggg
      721 ttaaaaacag cggagcttgt ggtaattgaa atttctcaac caataaagca cgattttgag
      781 cgatatcttg tggctcgtca ttaacgtgat cacctaagtt gaaactgtca aaaggcggtt
      841 tgcttactcc gcctgttctt aaggtcgtca gtgcgtgaat atgtttaggc acattccaat
      901 ttggaataat tttatccatt tgcaaaaaat ctcaagaaaa tgaccgcttg ttatcggcgt
      961 actgcaatcg cttcgatctc aatgccaaca tcttttggta aacgagccac ttctacgcaa
     1021 gaacgagccg gaaaattagg gtgttcgttt tcttggaaga aggcttggta ttccgcattt
     1081 acctttgcga agtcgttaag atctttgaca aatacggtgg tctttacaat atcggcaacg
     1141 gttaagccgg cttgttcgat aatggctttc acgttgttca gagattggcg ggtttgttgc
     1201 acaatatcac tcgggatttc gccggtttca ggattcacgg gaatttgccc agaagttaaa
     1261 accatattgc ctaaatcaac tgcttgcaca taaggcccaa tcgctgccgg tgcattttct
     1321 gtgtggataa ctgtcatctt gtgttcctct taatgaaaaa taaacggatt aatgataata
     1381 tcttttctta ttatttggaa taaagagaag tgaattaatg agcattaatg tttctgaaat
     1441 cgtgttacat caattacaaa aaacagaggg cgagaacccg gagcttaata cgtttttgcg
     1501 agaaaattta ttggcaattt cccctgaagt cgatcaaatg atgttgcaat tacatcaagc
     1561 ctatcaaagt aaagcaaagg cttacggtat atttaaaccg gagtcggttt tcgctcaaca
     1621 gctcaaccgc ttgttggagc aagaaaccga ttttttacct tttagccata gctgtgccaa
     1681 aatgcttgca gcagagctga ttaaatatcc gtttgccgat ggtggcactt ttattttgtg
     1741 tcgttacact tttttagcta ccgaatattt gtttattgcg ttgattgata gccgtaattc
     1801 aatgctggtt gatgagcagt tagagattaa acgcacgcaa tatctcgaaa ttgcacaata
     1861 tgatattgcc tgccgcatta atttaactga actgaaactt gacgcaacgt caaatcgcta
     1921 tttaactttt gtgaaaggca gagtagggcg taaaattgcc gatttcttta tggatttttt
     1981 aagtgcggaa gaagggttta atccacaatt gcaaaatcaa accttgttgc aagcggtgag
     2041 cgattattgt gagcaagggg agttatctgc ccctcaaact cgagaagtga aaaagcaggt
     2101 gtttgaatat tgtaaagggc agataaatag tggagaggaa attgcgttaa atgagctttc
     2161 caacgcattg ccaacactga atgaaatcga ttttgcccaa tttacgcaag agcaagaata
     2221 cggcttggaa gagaatattc cgcctatgcg aaatgccctt aaaaccctga cgaaattttc
     2281 cggttcgggc aaaggagtaa cgattagctt taacgcagaa ttattaggag agcgtttact
     2341 ttgggatgaa ctaaacgaca cactgacaat caaaggatta ccggcaaatc tgcgtgacca
     2401 gctggaacgt aacaaataat ggaaaatttt tatcagttta ggtattatct cgttatctga
     2461 attttggatt atcaaaaggt caattatgaa aatgaaatct cttttagctg caatgttgct
     2521 tgcactgggt gtaacggctt gttcaaccgt tgagaaagtg gtgtaccgta ttgatgtacc
     2581 gcaaggcaat tacttagaac aagataaaat tgctcaactt aaagtcggta tgacagcgga
     2641 acaagttgaa tatttgcttg gtacaccaat gttaaagaac atttttgaaa cccaccgttg
     2701 ggattatgtg tttattaaac gtgaaggtta taaagatcct gaacaacgca atctgtttat
     2761 ttattttgat gctaaagggt tagtatcaaa aattgatgac agcaatgcga atgcagaaca
     2821 aaaataggac gatttgttgg ttcgaaaagc ccacttttaa aagtgggctt atttttttga
     2881 ataaaaaata agcatttaaa cttaattttt cttattttaa tgataaattt ttctaatcta
     2941 tcttttcaaa gctaaaattg cttgctagac ttcgctcatc ttaaatacac aacatcatca
     3001 atttattcag gagtataata tgagcgattt aaacgccctt ttccaaaaaa tcaaacaacg
     3061 tgatccgaac caagcccctt tccatcaagc ggttgaagaa gtgttcggta gccttgcccc
     3121 tttcttagct aaaaatccga aatataccca gcaaggttta ttagggcgta ttgttgagcc
     3181 tgagcgtgta attagcttcc gtgtcacttg ggttgatgat aaaggtcaag tgcaagtaaa
     3241 ccgtggttac cgtgtacaaa tgaactcagc aatcggtcca tacaaaggcg gtattcgttt
     3301 ccacccaacc gttgatttag gcgtattaaa attcttagcc tttgagcaag tattcaaaaa
     3361 tgcgttaacg acattgccaa tgggcggtgg taaaggtggt tcagactttg acccgaaagg
     3421 caaatctgat gccgaagtaa tgcgtttctg ccaagcgttt atgagcgaat tatatcgcca
     3481 cattggtgct gatactgacg ttcctgcagg cgatatcggc gtgggaggtc gtgaaatcgg
     3541 ctatatgttc ggtcaataca aaaaattacg caatgaattt acttcagtat taaccggtaa
     3601 atcgctgact tggggtggta gcttaattcg tccggaagca actggttatg gtgcggtgta
     3661 ttttgcagag tctatgttag cgactaaagg tcagcagatc gaaggaaaat cagtggtaat
     3721 ttccggttcg ggtaacgtgg cacaatatgc agctgaaaaa gtgattcaaa aaggcggtaa
     3781 agtattaacg gtttcagatt ctaacggtta tgtcttattc cctgaatccg gtatgacaga
     3841 ggcacaatta caagcattat tagagttgaa aaacgaacgc cgtgaacgct tatctgtgta
     3901 tgcaacagaa caaggcttaa aatactttga cggtcaaaaa ccgtggggtg tgaaatgtga
     3961 catcgcttta ccttgtgcaa cccaaaacga attagatacc gaagatgcta aagaattagt
     4021 gaaaaacggc tgtatttgtg tggcagaagg tgcaaatatg ccaaccacat taggtggtgt
     4081 tgaagtcttt atcggtgcga aaatccttta tgctccgggt aaagcggcta acgcaggtgg
     4141 tgtggcaacc tcaggtttag aaatgagcca aaatgcaatt cgtctatctt ggagtcgtga
     4201 agaagtagat cagcgtttat tcaacattat gaaagcaatt cacgaaaact gcgtagaaaa
     4261 tggtactgaa gcagatggtt tcgtaaacta tgtaaacggt gcgaatattg ctggtttcaa
     4321 aaaagtggca aatgcgatgt tagaacaggg tattgtttaa tttattatcc ctattattca
     4381 agaactgcct cgatatatcg aggcagtttt tttattttag gttgtagcta gagacaagct
     4441 aaattgaaat cgtatttcat ttgccaatta tggtaaaatc tatgccaatc tcagttttag
     4501 cagtatgtga aagggcagta tatgatcatt acgatgaaag atatcgcaaa ggaagcaaat
     4561 gtttcgttag gaacagtatc gaatgtatta aacggaaaaa gtaatgttag cttagctaaa
     4621 atagaaaaag tacaagctgc cgttgaaaag ttgggttatc gtaaaaatat tcaagcctct
     4681 acgttaaaaa gtggcacaag taataaaatt gctattatat tgcccaatat tacagagctg
     4741 aaatatgcct gtttatatga aaatcttgat ttaatttgta cgcaaaat
//
LOCUS       DANLUA010000180         4760 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLUA010000180 DANLUA010000000
VERSION     DANLUA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(62..772)
                     /locus_tag="PXB44_002229"
     CDS             complement(62..772)
                     /locus_tag="PXB44_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL5282666.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
     gene            complement(741..887)
                     /locus_tag="PXB44_002230"
     CDS             complement(741..887)
                     /locus_tag="PXB44_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282667.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            complement(1005..2222)
                     /gene="fabB"
                     /locus_tag="PXB44_002231"
     CDS             complement(1005..2222)
                     /gene="fabB"
                     /locus_tag="PXB44_002231"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL5282668.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2483..>4760
                     /locus_tag="PXB44_002232"
     CDS             2483..>4760
                     /locus_tag="PXB44_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5282669.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMRKLYSVFEALPGKISLIN
                     HKISSNLAEKAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWG
                     YFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVI
                     ALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGED
                     AILKALKLVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQE
                     NEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQ
                     QISEFASEGFLQFFFEDNV"
BASE COUNT         1529 a          976 c          784 g         1471 t
ORIGIN      
        1 ctaaaaatga ccgcttgcag gcaataaaaa agtgcgtttc cgcacttttt actaaaatga
       61 tttaattact caaccaatgc tcaccgttgc cattagctgg tggattaatc ggttggttat
      121 tattcttacc tggaataata atttgaggct gatcgtacat acatttgttg ttcgacaata
      181 tatcacaggg aatagcacat acggcagggg cagaaagaag acaacttgaa ccaccttttt
      241 gattacaatt ctctaatgca ttttcttcag caagactact aacgatacca tacccaaaat
      301 cctcgaagcc ttgaccatct ttataacctt gtgcaaaagc aaggcacata ttactaaacg
      361 ataagatcat ttggcaatta cgaccaccgt tcttttcaca ctgtttaaac gcatcttgct
      421 tcgcattttc taaattttta gacgtattat cagtcaccca catcatattt tgttcaggtg
      481 acatataaat tgatgaccat aaacgaggct gtgttttacg ctttgctaag tcctctttac
      541 tggcaatggt taccgcttct ttatattcac cacatttaaa ctctttttcg acatcaccaa
      601 aaccattcat ttcacgaaca gaagtgattt tattatcagc gtcagcaaaa agatatgtcc
      661 aagtcgatcc acctgtataa accttatctg ttgtcataaa tatagtggtt gcattacgac
      721 caacttcata agtttgcaaa ttatacaaag ctagttgaat aaccataaac atagcttaat
      781 tcattatctt ttacgcattg aaccgctgta tcaggtaaac gtttacgtgc aaaaaattca
      841 gatacatctt taccacgcca ataaacacta tctagtggct taatcattgt agaacaagcg
      901 gtcaaaaata atgacaaaat aacaaatagc aaagaactta ttttgaaatt tataataatt
      961 acctcaaaag aaaaaagagc aagctttcac ttgctctttt tatgttactt agaatagcgt
     1021 tggaacacta aacacgcatt agtaccacca aagccgaagc tgtttgacat cacggttgtt
     1081 aatgctttgt cttggcgttc agtcacaata ttcatacctt gtgcttgttc atcaagggtt
     1141 tcaatattaa tgcttggtgc aataaaatta ttatctaaca ttaataatga ataaattgcc
     1201 tcgtgcgcac ctgctgcacc taatgagtgt ccggtcattg atttggttga tgaaattgct
     1261 ggcgttttat caccaaagac attacggatc gcccctaact cttttacatc acccactgga
     1321 gtagaagtgc cgtgtacgtt aatatactcg atttcaccat ttacagttgc catcgcctgt
     1381 ctcatacaac gctcagcacc ttcaccactt ggcgcgacca tatcgtaacc gtctgaggtt
     1441 gccccatagc ccacaatttc agcatagatt ttggcaccac gagcaagagc gtgttctaac
     1501 tcttccacca caaccactgc accaccaccg gcaatcacaa aaccgtcacg attagcatca
     1561 taagcacggc tagcttttgt tggtgtttca ttatatttag ttgaaacagc acccattgca
     1621 tcaaactcgg ttgcacattc ccaagaaagt tcttctgcac cacctgcaaa caccacatct
     1681 tgtttaccta attggattaa ctccatcgcg tgtccaatac agtgtgctga agtcgcacaa
     1741 gctgagctga ttgaatagtt tacaccacga attttataag gtgttgccaa gcatgctgac
     1801 acacttgatg ccattgtttt ggttactgcg taaggcccaa cggctttcac accacgtggg
     1861 ccacgtactg catcacaagc tacaagttga ctgtgcgccg agcctgtacc agctccgatc
     1921 accaaaccgg tacgatcgtt tgaaacttgt tcttcggtta agcctgagtc ttcgattgct
     1981 tctttcattg aaagataagc gtaagccgca gcatctccca taaaacgata gattttacgg
     2041 tcaattaact cagccggatt taatttaatc gtacctgcca cttggctacg catacccact
     2101 tcagcaaact caggcacaaa ttcaatgcct gatttacctg cttttagtga ttccaacact
     2161 tcttctttgt tgttaccgat actggaaatt acacctaatc ctgtaataac gactcttttc
     2221 attttgtttc cttcatcatt ttgatctgaa aaatacttga ttcagcttac acttgttcac
     2281 tcaaataagc tactaaaact tgattatttt acacaatttc attactggtc tgaactcttt
     2341 ctatcaagat agcggttgaa tttcagacat tttttgcaaa atagggaatg aaatttaatt
     2401 tcccgatttt ttaccctatt acataataat cacttttccg ctaaaatgct aaacagttca
     2461 cactttagta aggaaagctg acgtgaaaac attaaccctg acagccaaca aaataacagc
     2521 cgtagaaggc gctgccattg gggcgaacca ggggagcttc gcccaaaaat taaactctac
     2581 taaagcacga attgatcaac ttaatgcata ccgaatagaa cgagctatca atgctaataa
     2641 tcatcatttt atgcatgttt tctctctcat tccattgctt attcatctta atcaccctgt
     2701 gttaccggct tatgttgaaa atgcaccaca agggatttgg cattttcaac tctctgatta
     2761 tcaaaaacaa tttctggttt cttaccgttt tgatgaagca atctctgccg atcatttggg
     2821 cccccatccc agctttgatg ctctttattc gatgggtagc accggtacta ttacccaaac
     2881 aagctgttct gatttagatt tatggctatg ctatccgaac cattttacaa ttgaacaata
     2941 taagttaatg gagctcaaaa ttaataaatt gcaagaatgg gcaaagacat tccaaatcga
     3001 catcaatatt tatttaatga acccagaaca atttaagagc caaacttata acagtagcgt
     3061 aaccgatgaa cacagtggtt cagcacagca tttttttcta ttagatgaat tttatcgttc
     3121 agctatttta cttgccggta agcgtttact ctggttgcat ttacataaaa atgaatacca
     3181 aaatgttcac aataaccctg aaattgactt aacggaatgg attgatttcg gcgatttttc
     3241 cagcctttca accagtgaat tttttggtgc aagcttatgg cagctctaca aaggaattaa
     3301 taacccttat aaatcggcaa ttaaaatttt attgctcgaa tgctacgctc acacttatcc
     3361 taaaacaaag ctcatctcca aagaatttaa gaaaaaacta ctaagtgaca atgctttgga
     3421 atatcacttt gatccctact tagccatgct tgaattggta acagaacact taagatcacg
     3481 caaagagtgg gtgaaattag accgcttgcg agtctgtttt tatgcaaaag cggttgaggg
     3541 agaaataaat aacaactgga gaagtcgtga tttagacgag ctggttaacc aatggtcttg
     3601 gtcagaaaaa caaattcaat tacttcaaaa ccgtcaacat tggaaaatta agcaagtgat
     3661 gaatcatgag aatatgctga ttgatcattt actacaaagt tatcgaaatt taattcattt
     3721 tgctcgaaaa tttcatatta atccgagcat tatgacaaat gatactgata ttttaatgcg
     3781 aaaactttat tctgtatttg aagcacttcc aggaaaaata tctttaatta atcataaaat
     3841 atcgtccaat ttagcagaaa aagcaattac atttattgaa gctaaagttg gatctgcgtt
     3901 acaacccggt tggtatttag ttaatcaggc acctaaaagt cgttatgact ctgaaaatcg
     3961 ttatgtccat tataataaaa gcccgattaa gcttattgct tgggggtatt ttaacggcat
     4021 tattaacgca gcaactaatt tacatactgt tagccctaat ttagatatcg agaaattgcg
     4081 acaatttatc accgacttgc gcttatcttt cccaattcaa gcgccggaag tgaccaatga
     4141 tgacatgcat caccctaatg aaattcgcaa tttggttata gcgcttaatt tagtacaaga
     4201 tccaaccgaa catatcgatg aactaccaaa aaaagtttat ccaagcgatc tgtttaattt
     4261 tggtaaggca caaaaaacgt tagttggtag tgtgagcatt atttatcgaa atgtatggaa
     4321 tgaaattcga actcaacatt ttgacggtga agatgctatt ttaaaagcac taaaattggt
     4381 atccaatcga atttatcaag gaaattcttc acctcaatca gttaatgttt tttgctatag
     4441 taaatatcta cgtagtgagt tacgtgattt tgtggcagat ttagttcata aatgtattac
     4501 aattcagact ggttgtttac tgcaagagaa tgaatttagc actttaaaag tagcaggaaa
     4561 aacctggcag tttctttttc ataaaaaaac atttagcatt aaacaaattg atgaacaaca
     4621 agcggttgaa aatttaaatt cttttgcaaa aaccaagcaa aatgctaaaa gcgaaaatga
     4681 aaagaaaaaa gaatttccgc agcaaattag tgaatttgcc agtgaaggct ttttacagtt
     4741 tttctttgaa gacaatgtcg
//
LOCUS       DANLUA010000181         4751 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLUA010000181 DANLUA010000000
VERSION     DANLUA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(62..1909)
                     /gene="selB"
                     /locus_tag="PXB44_002233"
     CDS             complement(62..1909)
                     /gene="selB"
                     /locus_tag="PXB44_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL5282670.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(1919..2215)
                     /locus_tag="PXB44_002234"
     CDS             complement(1919..2215)
                     /locus_tag="PXB44_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282671.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(2212..2388)
                     /locus_tag="PXB44_002235"
     CDS             complement(2212..2388)
                     /locus_tag="PXB44_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282672.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(2457..3089)
                     /gene="pdxH"
                     /locus_tag="PXB44_002236"
     CDS             complement(2457..3089)
                     /gene="pdxH"
                     /locus_tag="PXB44_002236"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL5282673.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(3147..4529)
                     /locus_tag="PXB44_002237"
     CDS             complement(3147..4529)
                     /locus_tag="PXB44_002237"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL5282674.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            4597..4691
                     /locus_tag="PXB44_002238"
     tRNA            4597..4691
                     /locus_tag="PXB44_002238"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4631..4633,aa:Sec,seq:tca)
BASE COUNT         1275 a         1078 c          949 g         1449 t
ORIGIN      
        1 agattttata attagtaaat ttattttttc gcataaaaaa atctatgcct aatgccacaa
       61 gctaaaatgt ttcactatct cttaacaaat gcacatcgcc ttttcgtctt aaaaaaccgc
      121 tacgatcaaa atattcaatt aattgtaccg tcaattttcg cccatactgc aactcatctc
      181 gcaactggtt caccgaaatg ctgccgtttt gggcgataaa atctttaatt aacgttgcaa
      241 attgcccgat ttgttcgctt aataaaaatc tatctttgac gattgggatt aaatagccca
      301 atttcccgac tttatacagc aaattccgca tttgggtttc atcaatcgag agctggcttg
      361 caatatccct cacccaaaga gcttgattgg ttgcctcaaa cagcggtcga atttgttgcc
      421 aaatttgcaa ttcatcggcg ttcaattcga ttcgatgttc cggcaaatgt aaccaaccac
      481 gactattgac caaggctttt tgctccacca aatcctcaat aaattgattt gccaagtttt
      541 caggcaaatc caataacgct atgcgatata aacgagcttt ggtcacgccg atttgatctt
      601 gatgttgcag gtggtattct gccatttttt ccaacacccg ttgctgggct tgagctttaa
      661 aatccgaatt aaataaccat ttagacgaga catcaaaccc cagttttgca atatcctctg
      721 caaaacattg ctccgcccat aacagcaagg ataaatccat cgctctgttt tgcagataga
      781 gatcaaggcg tgtcgcaaag tcattacaag cggtggtttt tgctaaattt tttaccaaag
      841 ctaaacgggc ttcggtacgt ttatgccgtt ttggtgagtg aatttccagc acctccgctc
      901 cggctaacgt taggctgtca tcaccgctgc ggatgatcaa cttatcacca acggcaatat
      961 gaagtggttc atctaaaatc acttctgcaa aaaattgctg atttttgacc gcttgttgcc
     1021 cctcaagcaa attcaatttg ccggtaatgt gcgaagcaaa atggtaaaga tgcacaatgc
     1081 tgttttcttt gaaggcttga ttggcagtga gttgaaccgt aatgcgatcg gtggcaaatt
     1141 tcggctcaag ctcggtaatc caatcgcctc gcttgatttc ctctttttcc acgttagcga
     1201 tatttaacgc caaacgttgc cctgccactc ccgtttccga aggcgtattt tgggcgtgaa
     1261 tcgctttgac ccgcactttt ttaccattcg acaaaaataa ctcagagcca acgctcactt
     1321 ttcccgccac cgttgtgccg gtaacgacca accctgctcc tttcacatta aacacacggt
     1381 caatcgcata acggaacggt ttatgggttt gggtatgttg ttgatttaat tgaattaaat
     1441 gtgcttttaa ttcttcaatc ccttgttgcg tttttgccga agtaacaaag gtttttgctt
     1501 ccgctaaaaa cggatagctt gttttcactt gctcaattaa gtggctgatt tgagtttctt
     1561 ccgcccgatc tgccttagta atcacaatca taatatgctg gaaattcagc aggtggagaa
     1621 tatccaaatg ctcttcggtt tgtggcttaa tcccttcttc ggcagaaatc accaacaggg
     1681 cgtgttgaat gccgccaagc cctgccagca tattagataa aaaacgctga tgacccggca
     1741 catcaataaa ccctaaaata tcgttctcaa tcggtaaata ggcatagccc aaatcaatgg
     1801 ttagcccacg tttttgttct tcgggcaaat gggcggtgtt agtgccggtt aaggcttgta
     1861 aaagcgatgt tttgccgtgg tcaacgtgac cggcggtgca gatgatcatt gatagtcctt
     1921 ataaaaatga gtaactattc tggcgagcag atttaaaatc tacaatatag acattttcct
     1981 gaccaatcag gaactttact tggtacttac cgtttaaaat gggaatttct tttttcttgc
     2041 gaagtcgata aacatcggca gtaaaggaaa gtttatccac ttcacttttt attgcatcat
     2101 aaaattgttc tgcaataatc gggttttggg attgttgtaa tatataatct gcttgataaa
     2161 acaattttat ttctgctttc tgtagccaaa caatattgta atcaaacttc atcatttttc
     2221 cgtgattccc aattttttgc gtaaatcttc catcacctga gcgtgggatt tagtttcaat
     2281 taagccattt tctaattgat gtaataaatc aagttcatca ttagtataaa aactttgtgg
     2341 cttatctgtg cgtaattttg ctaaatcttg tgcgagtatg agttccatat ttccccctta
     2401 caaacttaga ttattcaata ttgtaacctg ataaaactaa agttcaagga gtgtatttaa
     2461 ggcgataacc gctctttttt ccactcatta ttttccaacc aataatgaat gcggtcgtgt
     2521 agccggctac ttctgccttg ccagagttca atactgtcgg gaatcagaat atagccaccc
     2581 caatgcggtg ggcgaggcac tgaaagtgga tatttcaatg caaattgagc tgattttagc
     2641 aacaaagcct tgtagttttc gataggctga ctctgctcgc tcgcccaagc ccccaaacgg
     2701 ctggtgtaag gacgagagtc aaaataggca tcggattctg cctctggaat ttttgctatt
     2761 ttcccttcaa tccgcacttg acgttgtaat tctgcccaaa agaaggttaa acatccaaaa
     2821 ggattatcgg caatttccca ccctttacgg ctttgataat tggtaaagaa cacaaagcct
     2881 tgtgaattta cctctttcaa cagcaccatt cggctacttg gtcgcccgtt tgctgcaacg
     2941 gtagcaatat tcattgctgt cggctcattc actttggcat tgattgcctc actcaaccat
     3001 tgttcaaatt gggtgattgg gtttgagtga caatctcgga ttgaaagcac ttttttggtg
     3061 taatcttctc gaatgtggtg cagatccata aggattcctt tatcttattc taaataatcg
     3121 gacaggcaca gtctgcccta ccaaaattaa tattccaaca tctcaatcaa cttatcaaac
     3181 tccgcgaccg agcgtaaatc taaccaaaat ttctgctgag caaaacgccc gataatcggg
     3241 cttgggtagg ttttaaattg ttgttccaat gcaattaaat cgctctgttt ttcagcggaa
     3301 atagtcactg ccaccgaagg taaggtttct gtgggtaacg caccgctgcc gatttgagcg
     3361 aggcttagct caatttgcaa aatataagca gaatttaacc gcttgtgtag tgcctgtttt
     3421 aatcgctctg ctttgtgttg caaggtttca agcggttgag tgagtaaatg aagagtcggc
     3481 aagttatcgg ttaattgctc cggaaataag tagtggcgta gcgttgcctc aagtgccgat
     3541 aaaatcactt tatcgcaacg tagcacccgt tttagcgggt gttgttgcag ttggctaatc
     3601 atcgcttggc taccgacaat aatgccggct tgcggcccgc ctaataattt atcgccagag
     3661 aaggaaacca aatctacccc agccgctact ttttgctgaa cctttggctc agcaggcaaa
     3721 ttaagagctt gcatatcagt tagcgaacca ctgcctaagt cggtaatcac aggcaggtta
     3781 aactctttac cgagagccac caattcctct tccgacaccg aactggtgaa cccctcaatg
     3841 tggtagttac tggtgtgaac tttcattaaa aaggcggtat tttcggtgat cgcgttgcga
     3901 taatccttta aatgagtgcg attagtcgta ccaacttcca ccaatttgca accggcttga
     3961 gccataatat ccggaatgcg gaatgccccg ccaatttcca ccagctcgcc acgggaaacg
     4021 atgacttcct tgccttgtgc gaaggttgct aacatcaaca acaccgctgc cgcattatta
     4081 ttcacaatgc aagcggcctc cgctccggta agtatttgca acaactcgga aatgtagtta
     4141 tctcggtgcg agcgcttgcc tgcttcaata tcaaattcca acgccacatt gtttcgcatt
     4201 gcgttggttg ctgcctgtat cgccttttcc gaccacaacc cacgccctaa gttggtgtgt
     4261 aacaccgtac cggttaagtt gaacacggaa cggattttca cctcactcaa cgcttgcact
     4321 ttttgttcca gccttataaa aaacaggttt tcatcggcaa taaaatccgg caaggcttgc
     4381 tgttgggaaa taaattgacg tgcttgagta agcaaactcc tagcttgttc agccacgctg
     4441 gaatatgcaa atttttggca taattcgacc gcttgtggct ttttcaacaa gaggtcgagc
     4501 gagggaattt ggcgaaatag ttgttgcata atgtgaatga ttgagagaat attttggaca
     4561 attctaataa ataaagtaca atattgccaa tttttcggaa gtgcgtcatc tccggtgagg
     4621 tggcaggact tcaaatcctg ttgaggacgc cagcgttctc gggtgggttc aactcccatg
     4681 cacttccgcc acatttcaaa agccgttaat ctttaacggc tttttgcatt tctgccacat
     4741 tctctctttc c
//
LOCUS       DANLUA010000182         4680 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLUA010000182 DANLUA010000000
VERSION     DANLUA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            133..267
                     /locus_tag="PXB44_002239"
     CDS             133..267
                     /locus_tag="PXB44_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL5282675.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            271..1434
                     /gene="torC"
                     /locus_tag="PXB44_002240"
     CDS             271..1434
                     /gene="torC"
                     /locus_tag="PXB44_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL5282676.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1534..3999
                     /gene="torA"
                     /locus_tag="PXB44_002241"
     CDS             1534..3999
                     /gene="torA"
                     /locus_tag="PXB44_002241"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5282677.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            3999..4589
                     /gene="torD"
                     /locus_tag="PXB44_002242"
     CDS             3999..4589
                     /gene="torD"
                     /locus_tag="PXB44_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL5282678.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
BASE COUNT         1347 a         1060 c         1153 g         1120 t
ORIGIN      
        1 ttgtaataga tgatttttat actgtttggc tgtttctaga acaacttctt tatatttttg
       61 attgatatca aggcaaattg tttctgaaga taaaataatt aacacttatt agttaataaa
      121 aaaggagttg aaatgcgttc agaactatta aaaacgcttt tgctatcagg gctgatttta
      181 ccgctattga tggcgtttgc cgtaggagct tacggcttta ttgtgtggat gttacaactg
      241 tttgttattg gtttaccggg agcgtagaaa atgttaggtt caatcaaagc attatttact
      301 cggcctgcaa ccaaaatcgg gttgggagtg ttagttggta tcggttttat tgccggagca
      361 attagctggc atcaatttaa taaagcgatg gatcatacca gcactgaaga gttttgcgtg
      421 agttgccact caatgcagca gccgttagag gagcttaaaa aaaccgccca ctggtcgaat
      481 aaaagcgggg taacggcaac ctgttcaagc tgccacttac cgcacgatta taccgaaaaa
      541 tatgcccgca aagtgcaggc aagccgtgag gcgtgggcag aattatccgg caaatataaa
      601 gaagaaggct catttgaaaa acaccgctta gaaatggcag agcgtgagtg ggcaagattt
      661 gccgccaatg gttcaaaaga gtgtaaggct tgccacagct atgagcgaat gaattttgat
      721 aaaatgggcg aagcagctcg taaggcaatg aaaccggctg cggagaaaga tcaaagttgt
      781 atggattgcc acaaaggcat tgctcaccac ttaccgaaag ccgatgaaaa tgcactgaaa
      841 tcgaagtttg atcagtttgt gaataaatcc ccaaaccaag gcaaatctta ctacaccaaa
      901 gggaatgccg aattatttct agacgaaggt ttaagccaag ctatcgggca attagaaacc
      961 gccgtgccgg tggcatttgt gcaagcgggc aaaaatgcgg atttggttga gctgacaatg
     1021 tggcgtaaag agaaaggttt cggtcgcatt tggtatcacg atttcgggaa aaacattacc
     1081 gatgcggttc taaccaaaga gtttatggca aatcagccga aatttgaggt gttggaaacc
     1141 aaagaagatc cgattaccgg cttgaattgg caaaaagtga aactacaagc gtggattggc
     1201 aaagctcaat tattagacga tgttacccca atctgggcga atgcagaaaa ccaatacaaa
     1261 acccaatgta gcacttgcca cagacagcct gatgtcgccc acttcgattc caacagctgg
     1321 atcggcttat tcaacggaat ggtcggcttt accaatatgg ataaacagac cggcaaagag
     1381 gtgctacgtt atttacaaat gcacgcctcc gatagtgaag aagcgaagca ttaataatat
     1441 tactcccctc tttactaaag aggggaatta atcgagcgtt gaataacatt ctctgtacta
     1501 gctcacgttt ttacaaataa agaaggacaa gatatgcaac aatctcgccg tcaattttta
     1561 aaaaatatgt ctgtgatggc tgccgctgtc acaatgccga atttcctcgt gcctcgcaat
     1621 gtgtttgcga gtgaagacct cagtcaatgg aaagtttccg gctctcactg gggggcaatg
     1681 cgtgccaaaa ttgagaacgg acgagtggca gaaatcaaac cgttcgagtt tgataaacac
     1741 ccgaccgaaa tgctcaacgg cattaaaggc ttaatttaca gcgattcccg tatccgctac
     1801 ccgatggtgc gtttagattg gctcaaaaac cgccataaca gcaacaccgc acagcgtggc
     1861 gataaccgct ttgtgcgggt aacgtgggac gaagccctcg acctgttcta ccaagagtta
     1921 gaacgggtac aaaaagagta cggcccgtgg gcgttacaca ccgccaatgt gggctggcgt
     1981 tcaaccggtc aattccacag ttgcggtaac catatgatcc gagcgattgc gatgcacggt
     2041 aacagtgtcg gcacggcagg cgactattct accggtgcag ggcagaccat tctgccgtat
     2101 gtgctgggtt caaccgaagt ctattcgcag ggcacgtctt gggaaatcat cttaaaagag
     2161 agcgaaaaca tcattttctg ggcaagcgac ccggtgaaga acctgcaagt gggttggaac
     2221 tgcgaaaccc acgaagccta tgcctacttg gaaaaactga aagagaaagt ggcagcaaaa
     2281 ggcgtgaatg tgatctgtgt tgatccggtg aaaagcaaaa cccagaattt cttaggttgc
     2341 gaacagcagt acatcaatcc acaaaccgat gtgccgttta tgttggcact tgcccacacg
     2401 ctctacaccg agaacctgta cgacaaaaaa ttcttggata tgtacacggt cggctttgaa
     2461 aaattcctgc cgtatctgtt aggcgaaacc gaagacaaag tggcgaaaac cccggaatgg
     2521 gcggctgaaa tttgtggtat ttccgccgac aaaatccgtg aatttgcccg aatgcttgcc
     2581 ggcaaacgca cccaatttat tttcggctgg gcaattcaac gccaacaaca cggcgaacaa
     2641 ccgtactgga tgggagcggt gctagcctca atgttagggc aaatcggctt ggaaggcggc
     2701 ggcatcagct atgctcacca ttacagctct atcggtattc ctgaatccgg tgcggcaatg
     2761 ccgggggcgt tcccgctcaa tattgatgag gggcaaaccc cgaaatacga caacaaagat
     2821 tacaaaggct acagcgacac cattccggtg gctcgtatga ccgacagctt gctccacgcc
     2881 ggcgaaacca ttgattacaa cggcaaaaaa gtgacttacg ctccgttcaa aatggcgatt
     2941 ttcaccggct gtaaccaatg gcaccgccat tccgagcgga acaaaatgaa acaggcgttc
     3001 caaaaactgg aaaccattgt ttcgatcaac tacagttgga cggcaacctg ccgcttctcc
     3061 gacatcgtgc tgccggcctg taccccattt gaacgcaacg atattgacgc ctacggctca
     3121 tacagcaacc gtggcgtgat tgcaatgcac aagctggttg atccactcta cgactctcgc
     3181 tccgactttg agattttccg agatctctgc cgccgtttcg gtaaagagaa agagtacagc
     3241 cgtggaatgg acgaaatgca gtgggtagaa aaactctaca aagattgtcg ccgtgaaaac
     3301 agaggtaaat ttgaaatgcc ggagtttgct gaattttggc aaaaaggcta tgtgctgttc
     3361 ccagagggca aaccttgggt tcgccacgcc gatttccgtg aagatccgga attgcacgca
     3421 cttggcacac cgtccggctt tatcgaaatt tacagcaaca aaatcgcctc tttcggctat
     3481 gacgattgca aaggtcaccc aatgtggttt gagaaagccg aacgttcaca cggcggtaaa
     3541 aattccgaca aattcccgta ctggttgcaa tcagttcacc cggacaaacg cttacactct
     3601 caactttgtg aatcgaaaga actgcgtgaa acttacagcg tgcaagggcg tgaaccgctt
     3661 tacattaacc cacaagatgc cgccaaacac ggcattcaag acggagattt agtgcgagtg
     3721 tttaacgacc gtgggcaagc gattgtgggg gcggtgcttt ccgacaactt cccgaccggc
     3781 gtagtgcgtt tacaagaagg ggcttggtat tcaccccttg acgaaagcgt gggggcgatt
     3841 gacacctatg gcgacccgaa cacaatgacc ctcgacatcg gcacgtccaa gttggcacaa
     3901 gcggtttccg ccaacacttg cttggtgaac atcgagaaat ttatcggcaa tgccccggct
     3961 ccgaatggct ttaacggtgc gattgaggta acggcttaat gacgttggca atcagtcgtg
     4021 aagaacggca atttatctat ggctggttta acgcaatgct cgcccgagaa ctttcggacg
     4081 agcagttaaa tgccttacaa gcgggcgaat ttgacgattt ttttgcatac ctcagcgaat
     4141 tgggttttgc cgaacaggtt gcaaaatttc aagcagaagt gaccgcttgt aggcagctgg
     4201 aatttcctcg tctagaactg gcggctgact ttgcccaact gtttttattg gaaggcaatc
     4261 tcagtgccat tccttacgcc tccgcctatt taagcggcga agcgttagcg aaaaatttgg
     4321 cggaaatgga cagactgctg ggcgaatttg ggctgcaagt taatcgagaa accaaagagc
     4381 cgagcgatca cctttgcgtt tatctcaccc tttttgaccg cttgcttgct gaaaaaccgt
     4441 tggaaacgca acagcaattt ctgcaaaacc aactgatgag ctggctccct gcgtggttcg
     4501 ccaaagccaa aaccgtcaaa actaacaccg gcgtttatcc ggcgttgcta aggttgtttg
     4561 aacaggtgtt gctgaaggaa caaagataaa gttatttatt tgagaatgat ttgtattgag
     4621 cgaattgaga aaggaagtta aaatttcaac ttcctttttt attggctgga tttatccaca
//
LOCUS       DANLUA010000183         4667 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLUA010000183 DANLUA010000000
VERSION     DANLUA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(12..167)
                     /locus_tag="PXB44_002243"
     CDS             complement(12..167)
                     /locus_tag="PXB44_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282679.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            complement(305..562)
                     /locus_tag="PXB44_002244"
     CDS             complement(305..562)
                     /locus_tag="PXB44_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282680.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            576..752
                     /locus_tag="PXB44_002245"
     CDS             576..752
                     /locus_tag="PXB44_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282681.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            756..902
                     /locus_tag="PXB44_002246"
     CDS             756..902
                     /locus_tag="PXB44_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282682.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            918..1124
                     /locus_tag="PXB44_002247"
     CDS             918..1124
                     /locus_tag="PXB44_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282683.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            1121..1897
                     /locus_tag="PXB44_002248"
     CDS             1121..1897
                     /locus_tag="PXB44_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282684.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            2012..2424
                     /locus_tag="PXB44_002249"
                     /pseudo
     CDS             2012..2424
                     /locus_tag="PXB44_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            2438..2593
                     /locus_tag="PXB44_002250"
     CDS             2438..2593
                     /locus_tag="PXB44_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282685.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            2673..3647
                     /locus_tag="PXB44_002251"
     CDS             2673..3647
                     /locus_tag="PXB44_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5282686.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            3734..3931
                     /locus_tag="PXB44_002252"
     CDS             3734..3931
                     /locus_tag="PXB44_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282687.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
     gene            complement(4410..4667)
                     /locus_tag="PXB44_002253"
     CDS             complement(4410..4667)
                     /locus_tag="PXB44_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282688.1"
                     /translation="MNNKQILSIAQKFRQNHKDSHPFMLERGYVRIYQGKAYGWCAEQ
                     EDANTECPTALIVSLSNEIFEATGGDDISGAEKWEKYNSNF"
BASE COUNT         1410 a          827 c          989 g         1441 t
ORIGIN      
        1 gttacaaatt gttaatcgtc cagttcttga gcagtttctt caatagttga ttgaactaac
       61 aatgtggctt gctcaagagg aatacctttt ccggattcta attcagctct gccttttgca
      121 attttttgag taaggaactc atcgtagcct ttttctttaa atagcatttt atgttccttt
      181 cacttattac aaggctttca ggcataggga ttatgttctc ctaaatgcga aatattggaa
      241 taatttacgc ttaaagcaat agagtgtcaa gagcagggtt tttggaagtg tggtttggga
      301 atatttaaaa attatcatca taagtttcat cgtagttatc gttttcatcg ctggcatcat
      361 tttcatcgga atattcttct tcgctatatt cctcttcgtt atatccatca tcaagttcag
      421 gttcttgttc ccttagtgat tctttagctt caacttcagg cataaattgc tggtaattgt
      481 agaaaaaagt gccatcgctg cctttttccc aaagtccggc gtagttgtat tttacatatt
      541 caggatcagc gtggcatatc atccctaatt gaggggtggc atatgttcgt atttaccaag
      601 gtgaggctta tggttggtgt gctgaaaaag gagaggcaaa tacagaatgc cctgaggcct
      661 tattggtgag tttaaccggc gatatttttg aagcagtcgg cggagataag ctgaaaggtg
      721 ccaaaaaatg gcagccttac aagaaagaat gacagatgga tacgacacaa aaacgtgtgg
      781 ctttattcat cattggatta tctgttgtga tgtggctaag aaagcataac tgctgtacag
      841 tgatatttgt attgttgttg gcagcgttag catttagcta cacgatggaa catttatatt
      901 agctggggaa ctgaattatg gtaaagaatg tggttcatga agagatatta cataaccgtg
      961 cagaattaca gcaagcggtt gaaaaagaag aattttgtgc aaaatgtgat aactcagtag
     1021 tattccattt acgtgataat tatcacgaat ttacgcttgg gttagatact atattgcagt
     1081 gtctgcattt tgctgaagag aagggagcta tccggatacc ttgaatattg agtattcact
     1141 caatcattgc caaggagatg gagtcgcatt ttatggtcga cttggtacag atgatctaat
     1201 ccagcttttc aatcatctca atccacagaa acgtaaacag aaaatgtttg ctaatttgct
     1261 aacgcacatt gagtcttggg aaacccacga tgatattggt tttgaaatta tacgaaatcg
     1321 ctttgggtat cgttactcgc attggaatac gatggagctt tatgctaaaa ctgcagatca
     1381 gttagtgttt ttcaatgata gtgatgctcg aagaaactgg tattttccga cttcaaaagt
     1441 gtataaatac aaaggcttgt gggatgagtt tattcaggat ttagaacagt atattaaggg
     1501 tacttcccgt gaaatggaat cggtcggtta tcaaattatt gaagcctcgc cgtatcaaac
     1561 ggaaaccgtt ttcaaattta atacggaaaa ctatctagta gaactgaaac gtaaaccttc
     1621 tgagttttat tcagagcttg ttgattgggc atttggtgat atgtgtgatt ttgaagcaac
     1681 tatagagcag atttgtcaag ggcaactctg ttatgctgat tttgaagcaa ctgttaccga
     1741 taagaaaagt gaaattcagt taggtcttgc tcgcttgagt ggaataagtt actcgccgaa
     1801 tgaccgttcg tttagtggtt atcgacaaga gcttattggt gaggcgattg cctctgctcg
     1861 ccataatgca gcacgttttg cctacttgca cgcctaattt gcagatttgt acttcaattt
     1921 gaccgcttgt agtggttaat tttcaccccg tggggaaata ttttccctat tgggcaagat
     1981 gtttctccac tttttaattt ggagaaatgt gatgaaaaat gaacacaatt tagagcaact
     2041 taaatatatt tgggataacc gcaaccttga aaataaagcc tatcaatacc tgtttcacat
     2101 ttgcattgag ccgatgaaaa aaggagattg gaatcgttat acgatttggc ttaattttca
     2161 cggcaactta atcaacgtgt cagacgttgt atatcgagtt gtggaatctc aaaagctcaa
     2221 ggatgacatc aaaatgtggt atgacaataa taccattgtc gtccgataaa agttgaaaca
     2281 acaggcttca tctcatattt actgcaccag ttctatcagg ggatagccta tattttactg
     2341 gaacagggaa tgattactga aactgaagca gccgagaaaa gttataactt cagagctgca
     2401 aattgttttc aacgtttgct ttaatggagg tgagataatg gaaactcgca ttgttaaagt
     2461 tatttacgat gatggtgatt atttctttac ccgaatcaat gctgatgaac aggctatccg
     2521 taattactac gccataggca gttcaatagg ttttgatgat ataagaaaaa ttatcgctgt
     2581 tgaaatttta taaaccaccc ataggggaaa ctatttcccc atagggtgat agcgtttccc
     2641 catttttcta cccgaacttt ggagaaacac ttatgaacac tcaacctaaa tttgatttat
     2701 accaacacat cactgaccga attgttgtag cactggaaca aggtacagcc ccgtggttaa
     2761 aaccgtggag ccatccggaa tgcaatatgg cattacctcg caatgcggta tctaaccgct
     2821 tgtattccgg tatcaataca ctcttactct ggattgctac tgctgaacat ggttacaaac
     2881 agtgcaagtg gattacagca aaaaaagcca atgaactcgg cggatatatc cgtaaagggg
     2941 aaaaagctac tgtggtgatg aactaccgtc ctgatgagag agagaaactc gatgaggaag
     3001 ggaatattgt gtatgacgag gaaggaaatc cggaaatgga acgacacttt ttcattaata
     3061 agcacaatct gttcaatctg gaccagtgtg aaaatttacc ggtggaactg tacgatcctg
     3121 tatcagaacc gaaacaacct aaatctgagc caaaccaata cgatattttt gctgagattc
     3181 ggcagattat caaaggaatg gatttgcagg tggaaatgaa gcctagcaat cgggcatttt
     3241 atcaaccagg tgaagataag gtcgtaatgc cggagatgaa gcagtttgca aatgctcaag
     3301 cgtttcatag tgtattgtta cacgaaatga ctcacgcaac gggggcaaat aatcggctta
     3361 atcgggaagg tattacttcc ggaaaggcta aatttggaaa caaagtctat gcttttgagg
     3421 agttgattgc tgaaatgggc ggtgcatttt tatgtgccta ccttggtttt gatactgtgc
     3481 ctcaaaatgc ggcgtatatt gaaagttgga ttcgagtatt gaaagaagat aaacgggcga
     3541 ttgtccgtgc ttccggaaaa gctcgtgaag cctgccaata catgttagac gcattaaacg
     3601 tcgcacagtt ggctgacaaa tacgatgtat ttgaagaagc tgcttaacaa atttgccgaa
     3661 aggcatatct caaggggaga cctatccctt tgggcatagt gtttccctat tttttattaa
     3721 atggagaaaa gctatgacaa aatcaactga aatacctcgt ggacatgata actgggttat
     3781 tacaggcaga gacaatgatg ggaaatggca taaagaacgt tttctttatt attctcgccg
     3841 tgaagccatc caacagtttc gtcgaaaaca caactgcgta ggtaaacata gtgttattga
     3901 atcaataaca cctcaatttg gctggtatta accggctttc ccgtggggag atactctccc
     3961 ttcggagcat tgtgtctccc tttttattca cttaactaga ggacacaatg ttcaatctaa
     4021 acttatttaa acgccctatt gagctttcaa cgcttgaatc ttggtctaaa ttctgtgatg
     4081 atgtcggtaa agttgcattt attgcgatac ctccgatgat ttatagtaat tatgatctat
     4141 gtatcaaaat aggaaacata agtgctttat tggcaattac ctatggattc ttattatcag
     4201 gcaggactat ccgtcaattt attgagaagg agaaatcaag atgggattaa caatcggctt
     4261 atggatcatt gcttttatag gctttgcact ggttttttgg gcaaatagct tactcaaaga
     4321 gcaagatgaa cgccttaaac agctaaaaaa atagctcaac tttacactca atacggcaca
     4381 tttggtgccg tatttcattc ttatcctaat taaaaattag aattatattt ttcccatttt
     4441 tcagccccac ttatatcatc acctccagtt gcctcaaaaa tttcattaga taagctgaca
     4501 attaaggcgg tagggcattc cgtgtttgca tcttcttgct ctgcacacca gccataggct
     4561 tttccctgat aaatacgaac ataaccacgc tctagcataa aagggtgaga atccttgtga
     4621 ttttgacgaa atttttgagc aatagacaaa atttgtttat tgttcat
//
LOCUS       DANLUA010000184         4662 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLUA010000184 DANLUA010000000
VERSION     DANLUA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1188
                     /locus_tag="PXB44_002254"
     CDS             <1..1188
                     /locus_tag="PXB44_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL5282689.1"
                     /translation="FSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTPI
                     VKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLGG
                     TGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWEN
                     LIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFAN
                     GLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIKS
                     IIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGAN
                     KDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAIV
                     KVL"
     gene            complement(1295..2596)
                     /gene="folC"
                     /locus_tag="PXB44_002255"
     CDS             complement(1295..2596)
                     /gene="folC"
                     /locus_tag="PXB44_002255"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL5282690.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(2606..3502)
                     /gene="accD"
                     /locus_tag="PXB44_002256"
     CDS             complement(2606..3502)
                     /gene="accD"
                     /locus_tag="PXB44_002256"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL5282691.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            3811..4593
                     /gene="mazG"
                     /locus_tag="PXB44_002257"
                     /pseudo
     CDS             3811..4593
                     /gene="mazG"
                     /locus_tag="PXB44_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         1416 a          949 c          958 g         1339 t
ORIGIN      
        1 tttagtcaca ttcaagctgc tccggcagac tcaattttag gcttaggtga agcgtttaaa
       61 gcagatagcc gtgaaggtaa aatcaacctt ggtatcggtg tttatatgac cgatgaaggc
      121 aaaaccccga ttgtcaaagc ggtaaaagaa gcagaaaaac gtttattaga gacggaaaac
      181 agtaaaaatt atttaaccat tgacggtgtt caagcattca atgcggcaac gcaagcgtta
      241 ttatttggcg aaggggctga agtgattacc tcaggtcgtg ctaaaactgc acagagttta
      301 ggtgggacag gggcattacg cattgcggcg gaatttatca aacgtcatac gaatgcgaaa
      361 aatgtatgga tttcaacccc gacttggcca aaccataacg gcatttttga tgcagtaggc
      421 gtgaatgtaa aaggctatcg ctactacaat aaagagacta acggtttaga ttgggaaaat
      481 ttaattgccg atttaagcca agcggaagcc ggagatgtgg tgttattgca cggttgctgc
      541 cacaacccga ccggaattga cccaacacct gcacaatggg aacagttagc agcattatct
      601 gccgaaaaag gctggttgcc gttatttgac tttgcttatc aaggctttgc aaacggttta
      661 gaggaagatg cttacggttt acgagctttt gtgaaaaata accgtgaatt gttggtcgca
      721 agctcattct cgaaaaactt tgggttatat aacgagcgtg tcggtgcatt taccctaatt
      781 gcagataatg ctgatgatgc caaccgtgct ttcactcaaa tcaaatcgat tattcgtgta
      841 ctttactcta acccatctgc tcacggtgca agtgcagtag cagttgcatt ggctgatcct
      901 gaccttaaag cacaatggat tgcggaatta gatgaaatgc gtaaccgtat taaagaaatg
      961 cgtagccaat tagtgcagaa attaaaagaa aaaggggcaa acaaagactt ctctttcatt
     1021 actgagcaaa atggtatgtt ctcattcagt ggcttaacac cggagcaagt ggataagtta
     1081 aaagatgagt ttgcaattta tgctgtgcgt tcaggcagaa tcaatgtagc aggcattacc
     1141 agcaaaaaca ttgatgctct tgccgatgcg attgtgaaag tgctttaatt aaaccttaaa
     1201 gatatataga ggcataagcc tgagtgcttg tgcctctttt ttatctttta ggtacaagtg
     1261 tggacacttg caccaacgtt gattattcgt agaactaggt ttccaaccaa agcataaaat
     1321 ctgctaccgt atgaaatgag ccgaacacca gcacaatatc attttcgttg gctgtttcaa
     1381 acaatactcg gcttgcctgt gccacatttt catagctaca agcggttgaa tttggcaaaa
     1441 cgtttaccaa tttcgctaac accgcctcgc ctgtttgtcc acgccaacaa tctagccctg
     1501 cacaatgcca ttcatcaata atattagcta atggctcgac aataccggat aaatccttat
     1561 cttctaacgc actaaacacc gcatagattt tttgatttgg ttgctttaac tcccgcagtt
     1621 tgtcagctaa ataacgtgct gcgtgtgggt tatgccctac atcaataatt acttttgcag
     1681 ccggtttagg ttgagaaaaa tgagtaaaat cggtatccgt cagggtttga aaacgccctg
     1741 tcatttgagc ttcatttaag gcttgtcgaa taatcttttc atcaatttca aatggcagtt
     1801 gcattaatac tgctaatgcg gtgccggcgt ttggaatcgg aataagcggg attggtaaat
     1861 cggtaaagga tttttcttca gaataccaat gaaacagatt attcttaatt tcaaactgcc
     1921 agtctttacc ccggtaaaaa gcgtgacaat ttaacgaatc agcaatctgt ttaatcgaat
     1981 gagggcaatc aggctcgccg atgacaaccg gaatattttc acggaaaatg ccggctttct
     2041 cacgcccgat gtcttcacgg ttatcgccta aaaatgcaac gtggtcgata tcaatcgaga
     2101 taatcacggc aaggcttgga tcaataatat tagtggcatc taatcgccca cctaatccga
     2161 cttctaaaat cacaatatcg agctgatttt gtttgaatag ctcaaatgca gacagcgtac
     2221 taaactcaaa ataagtgagt gaagcggtct tttttgcatt aatttttgca aatgcttgga
     2281 cgtggtcggc atcaggtaat acttcgccgt taattcgcac ccgttcatta tagtgaatta
     2341 agtgcggtga agagtaaaca ccaacccgaa atccggcttt taataaggta acttctaaca
     2401 aacggcaggt cgaacccttg ccgttagtac cggcaacggt aatcacataa ggggcaggct
     2461 tgagtaaatc cagctctgtt gcgaccttac gaatacgatc taagcccata tcaatcggtt
     2521 tagaatgggc ttgttctaaa taggaaagcc acgtttccaa agaatccgtg gctttaggta
     2581 tgcttaatgt attcattcag atattttatt ctgcgttttc tacaaccaat tccgccactt
     2641 taaacggcgt tggttgattg gttaattttg cacaaagacg ggaaagggta tcacgcatct
     2701 ctttacgatg cacgatcata tcaactgccc cgtgttctaa taagaactcg gcacgttgga
     2761 aaccttccgg cagtttttca cgtacggttt gctcaattac acgaggacca gcaaagccga
     2821 ttagggcttt tggctcagca atattcacat cgcccagcat tgccaaactt gcggaaaccc
     2881 cacctaaagt tgggtcggtt aatacagaaa taaacggtac gccctgctct ttcattttgg
     2941 ctaaaatcgc actggttttt gccatttgca ttaaagagaa taacgcctct tgcatacgag
     3001 ccccgcccga tgccgaaaaa cagataaacg gaattttttc agctaaagct ttttccgcag
     3061 ctttcacaaa ttttgcacct actacagacc ccattgaacc gcccataaac tcaaagttaa
     3121 aggaggccgt aaccaccggc atattatgca atgtaccggc aagcacgata aaagaatctt
     3181 tctcaccggt ttgtttttgc gcggcagtta aacgatcttt atatttcttt aaatctttaa
     3241 atttcaacac atcttccggc tctaagtcgg cagcgatttc gaccgcagaa tctttatcta
     3301 acaaattcaa taaacgggtg cgggcatcaa tacgcatatg atgatcgcat tttggacaaa
     3361 cctgttggtt gcgttgtaat tcttcgctat agagaacttg ttcacaactt gtgcattttg
     3421 tccatacacc ttccggcact tttgattttg cattgcttga tgatgaagag tctctaccta
     3481 aaattctgtc taaccagctc attattattc cttttggatt gagttgttgg gttcactaaa
     3541 tggttgctat taaagcataa tttatcggtt tttgatatgt tttcttgaca tatcagatca
     3601 ctaactatgt gtataagttc gataaaaaaa cgcaatcaga agaatgatta acatcaccac
     3661 aatttgaatt aagcgttttt tggttaattt ttccgctgcc attgctctgc tcatacttaa
     3721 taaatttccg cattataagc gaaaaagcac gggctatgta gaatttgcaa aattttgatc
     3781 aaaaataacc gcttgtaaaa aggaaatact atggcaacca ttgaacaatt tcttgaggtt
     3841 gtaaaccaac ttcgtcaccc tgaacacggc tgcccgtggg atctcaaaca gaattttgac
     3901 acgatgttcc ccacttattg gaagaaacct acgaagtagc ggaagcgatt cacacccaag
     3961 atcgcactgc cttgcgtgaa gagttaggcg atttattact gcaagtggta tttttaagcc
     4021 aattagcgaa agaggaaggc agtttcactt ttgatgatgt agtaacggat attcacgata
     4081 aactgattta ccgccaccct cacgtttttg gtgatgtgaa agctactaac agcgaagagg
     4141 ccttacaaag ttgggaagca caaaaagcga atgatgcaaa acaccgtagc caagattcta
     4201 tttttagatg atttaccttt tgctttgcct gctcttaccc gtgcaaataa gctacaaaaa
     4261 cggtgtgcaa aagttggatt cgattgggat aaccctcacg atgtgctggc aaaagtcgaa
     4321 gaagaactcg atgaagtcaa agcagaaatc gcacaatatc cggctcgctc catcgagctt
     4381 gctgaagaat taggcgatct cttgtttgcg attgtaaatt tatgccgcca ttatcatacc
     4441 gatgccgagg aaattttacg caatgcaaat ctaaaatttg aacatcgctt tcgaaaagtt
     4501 gaaaaatctg tgaaaaaatc gggaagaact gtaaaagaat gtaaattaga agaactagat
     4561 gtaatttggc acaaaataaa agtggtaaaa taaaaagcac caattgttat actaattttt
     4621 acctttgtaa agttagtttt aattttatag ggaatatttt at
//
LOCUS       DANLUA010000185         4592 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLUA010000185 DANLUA010000000
VERSION     DANLUA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            209..4105
                     /gene="purL"
                     /locus_tag="PXB44_002258"
                     /gene_synonym="purI"
     CDS             209..4105
                     /gene="purL"
                     /locus_tag="PXB44_002258"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL5282692.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4460..4592
                     /locus_tag="PXB44_002259"
                     /pseudo
     CDS             4460..4592
                     /locus_tag="PXB44_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1288 a         1036 c         1129 g         1139 t
ORIGIN      
        1 ttgtcaaatt aacttatttt taaattatct tttaaaacag attgatagag gggaccttct
       61 ttgaaaggta ttcattagta ttgaacaaat ttatttgata ctaatgaata aatgtatcta
      121 acagcaaacg tctgacaaat atagcaatcg tttgcgcaat cgtttataat cggtttagtt
      181 tttcatctta aataggaaaa tccaaagaat gacaatccaa accttccgtg gctcgcctgc
      241 cttatctgct tttcgcattc aacaacttct gcaaaaattc caacaaaatc aattacctgt
      301 aaaatcagtt tatgcggagt ttttgcattt tgtggcatta aatcgaccgc ttgtagccga
      361 tgaagaaaac aaaatcaaag agttattgca ctacggtcca acgctggaag aagacgagcc
      421 gacagggttt tgcctgattg ttacccctcg tatcggcacg atttcctcgt ggtcgtctaa
      481 ggcaaccgac atcgcccata actgcggttt ggcagcagta gagcgaattg agcgtggcac
      541 ggcatattat ttagaatttt ctgaaacgcc gaacgaaagt caacttacga cgttgaaagg
      601 cttattacac gaccgtatga tggaaacggt gctggataac gaaagccaag ccgaacagct
      661 tttcgctcag caagagccga agccgtttac ttcggtggat attttaggcg gtggtcgcaa
      721 ggcactggaa gaagcgaata tcaacttagg tttagcgttg gcggacgatg aaattgatta
      781 cttggtggaa aactttaccc aacttgggcg taaccccaat gatattgaac tctatatgtt
      841 cgcccaagcc aactcggagc attgccgcca caaaattttt aatgcggatt ggacgattga
      901 tggggtaaaa caggaaaaat ccctgtttaa gatgattaaa aacacctttg agaaaacccc
      961 ggactatgtg ctttccgcct acaaagacaa tgcggcggtg atggagggct caacggttgg
     1021 gcgtttcttc cccgaccaag atggacaata tcgctatcat caagaagatg ctcatatttt
     1081 aatgaaagta gaaactcaca accacccaac cgccatctcg ccattcccgg gagcggcgac
     1141 cggttcgggc ggtgaaattc gtgatgaagg ggcgacaggg cgtggggcga aaccgaaagc
     1201 gggcttgacg ggtttttcgg tctctaactt agtgatccca aaatttgaac agccgtggga
     1261 aaacccgctt tccaagccga atcgcattgc ctccgcactg gatattatga ttgatggccc
     1321 actaggcgga gcggcattta acaatgaatt tggtcgtcct gcgttattgg gctacttccg
     1381 tacttacgaa gaaaaagtga acagcttcgc aggcgaagaa attcgtggct atcacaagcc
     1441 gattatgtta gcaggcggta tcggcaacat tcgtgccgaa cacgttcaaa aaggcgaaat
     1501 cccggtggga gcgaagttga tcgtgcttgg tggcccggca atgaacatcg gcttaggcgg
     1561 tggggcggca tcgtcaatgg catcggggaa atcgaaagaa gatttggatt ttgcctcggt
     1621 tcagcgggaa aaccctgaaa tggaacgccg ttgccaagag gtgatcgacc gttgctggca
     1681 gatgggggcg gagaacccaa ttttatttat tcacgacgtg ggggctggcg gtttatcaaa
     1741 tgcgatgcct gaattggttc acgacggcga atgtggcggt aagtttgatt tgcgtaacat
     1801 cttgtgcgat gaaaaaggaa tgtcgccact tgaaatttgg tgtaacgaat cgcaagagcg
     1861 ttatgtgctt gccgtttccc ctgaaaaact cgacattttc acagcattgt gcgaaaggga
     1921 aagagcccca tttgcggtga ttggcgaggc aacgaaagag aagcatttaa cccttgctga
     1981 ccctcatttt gacaataaac cgattgattt gccgatgaat gtgttgctcg gcaaaacgcc
     2041 aaaaatgcag cgagatgttt tgtcaaaaac tgtgaaaaat ccaccgcttg caaccgctga
     2101 catcgacttg aaagaggcgt tccaccgtgt gctacgcttg cctgcggtgg ctgaaaaaac
     2161 cttcctgatt accatcggcg atcgctcagt aacgggaatg gtggcacaag atcagatggt
     2221 cggcccgtgg caaattcctg tggcagattg tgccgtaacc accgcttcat tagacagcta
     2281 tcacggtgaa gcaatgtcaa ttggcgagcg atcacctgtt gctttgcttg actttgctgc
     2341 ctcagctcgc ctcgccgtgg cagaaagcat caccaatatt gccgccacta acattggcga
     2401 catcaaacgc attaaactct cggcgaattg gatgtcggcg gcagggcacg agggcgaaga
     2461 cgcagggctt tacgaggcag taaaagcggt gggcgaagag ctttgccctg aattaggcat
     2521 aaccattccg gtaggcaaag actcaatgtc gatgaaaacc acttgggagg aaaacggcga
     2581 acagaaatct gttactgctc cgctttcgct cgtgatttcc gctttcgctc gtgtggaaga
     2641 tgtgcgtaaa accgttaccc cacaacttcg caccgacaaa ggcgataccc gcttgttgct
     2701 gatcgacctg ggcgaaggca acaaccgctt gggtgcaacc gcacttgctc aagtttacaa
     2761 acaacttggc gacaaacctg ccgatgtggt gaatgtggct cgcttaaaag gcttcttcaa
     2821 cgcaatgcaa acccttgtcg cacaaggaaa attgcttgcc taccacgacc gctcggacgg
     2881 tggtttaatc accacactgg cggaaatggc gtttgcaggg aattgcggaa tttcggtgga
     2941 aatcgaccgc ttggtggacg atttagccgt gttattcaac gaagaactgg gggcagtaat
     3001 ccaagttcgc accgaagaat tgagcgaggt gcaagcggtg ttaaatgccg aaaatcttgc
     3061 aaatctcacc tttgatattg gtacagcggt tgaaagcgac agctttgaaa ttacccgcaa
     3121 cggcacaaca gtatttagcc aaaaacgttc cgaactgcgt ggcatttggg cggaactcac
     3181 tcatcaaatg cagcgtttgc gagacaaccc tgaatgtgca gaccaagaat ttgcagcgaa
     3241 aaaagacccg aataacaaag gcttatccgc atttttaact tacgatgtga acgaagacat
     3301 cgccgcccca tacatcaaca aaggcgtaaa accgagtgtg gcgattttgc gtgagcaagg
     3361 tgtaaacagc cactacgaaa tggcagccgt ctttgaccgt gccggtttca acgccattga
     3421 cgtgcatatg tcagacttga tttcaggcag acgcaacctc aacgacttca acgcaatggt
     3481 ggcgtgcgga ggcttctctt acggcgatgt cttaggtgca ggcggcggct gggcgaaatc
     3541 gattctgttc aacccaatgc tgcgtgacca attcagcaaa ttcttcgcca acccgaacac
     3601 gctggcatta ggggtatgta atggttgcca aatggtctcc aatctagcgg aaattattcc
     3661 aggcacagaa aactggccac gcttcgtgcg taataaatcc gaacgttttg aagcccgtgc
     3721 tgcaatggtg aaaatcaacg acaccaactc actttggttc aacggaatgg caggcagcca
     3781 tatgccgatt gcggtttcac acggtgaagg acaagttgag ttcaaaaatc cacagcaatt
     3841 agcgaacttg caacagcaaa atctcgtgat cgcacaatat gtggatagcc acctcaaccc
     3901 aaccgaacaa tacccagcca acccgaacgg atcggcgttg ggtatcaccg ctatcggtaa
     3961 tacagacgga cgagtcgcta ttatgatgcc acaccctgaa cgtgtgttcc gtgcggtcag
     4021 taactcttgg tgcccagaag attggacgga aggtggagcg tggatgcggt tgtttagaaa
     4081 tgcgagagtg gcgttgaagt attaaaaatc taccttcgta gtgatctgcc cccatagggg
     4141 gcgattaaaa ttcgaattca tagaacccga atttttatat atctgataaa cgaagttgtt
     4201 tatccttcgt gcttttcctg atatttcaag tactttctta tcatttctgc attgacatct
     4261 acggtgtcta cgcagtagcc tcttgccaca aaagtgggtt gccccacaat ttatgcttac
     4321 gtaaataggg aaacttattg aaaaatctta ttggaccctg ttcacccttt taaatgcccc
     4381 attacgtctt atacagaaaa tgaacgtgat ttatttgaac ggtcaattct accttaatag
     4441 aaagtggttt ttagttagca atcaaaatca cattaacgga attgagaatt tggagagtca
     4501 ggcaaaatgc tatttgcgta aatttaacgg cgttcctaaa gtacatgaat tctatttgaa
     4561 agaatgcaga tggcgtttta atcacagtaa ct
//
LOCUS       DANLUA010000186         4491 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLUA010000186 DANLUA010000000
VERSION     DANLUA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            210..1529
                     /locus_tag="PXB44_002260"
     CDS             210..1529
                     /locus_tag="PXB44_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL5282693.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(1699..3642)
                     /gene="ftsH"
                     /locus_tag="PXB44_002261"
     CDS             complement(1699..3642)
                     /gene="ftsH"
                     /locus_tag="PXB44_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL5282694.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(3763..4389)
                     /gene="rlmE"
                     /locus_tag="PXB44_002262"
     CDS             complement(3763..4389)
                     /gene="rlmE"
                     /locus_tag="PXB44_002262"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL5282695.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         1228 a         1020 c          830 g         1413 t
ORIGIN      
        1 accagtaaat tttttgttac aactggaaag aaattggtac aaacattcgg attagctttg
       61 caaaaagcgt aaaaaatctt accgcttgtc atttatcagt tagggaaaat aaaaaatcac
      121 tctcttatat aaaaacaatt acgcaaacga ttgcttatta aaaaaacttc agtataatgc
      181 cgacattttt taactctcga ggaatcacta tgaaccattt actttattct gtcgaagaca
      241 aaccgccttt cgggctgagc ttattgcttg cctcacaaca tttattagcc gctttaggtg
      301 gcattatcgc tgtaccttta gtgctaggga atgtactcaa actctctact gcggatacta
      361 taatcttagt taatgctgca cttttaattt caggcatcgt cactattatt caatgtcgag
      421 gtattggccc aatcgggatt agactaccga gtgtaatggg aactagcttt acgtttgttg
      481 cagcagcatt agcgattggt ttcagtgaaa gtggcattgc cggcattatg ggagcctctt
      541 tagttggctc tctagtgatg attatcggca gtttttttat gccttatatc cgtaagcttt
      601 ttccacccgt ggtaacaggt actgtcataa tgatgattgg cctaagttta attcctgttg
      661 cagtagactg gtttgctggt ggtcagcgta ctgatccaca ctatgcaacc ccagaaaatc
      721 ttataatggc tagttttgta ttaattattg tcgttgcttt agtacaatgg gggaaaggta
      781 ttttttcagc tgctgctatt gtgattggta tgatgatagg ttatatcgtt tcgctagctc
      841 tgggttgggt aagttttaat ggcgtgaaag aagcacaact ttttgcgtta ccacaacctc
      901 tacattttgg cttagaattt ccaatctccg gtatcatcgg aatgtcaatt gcttatttag
      961 ttaccattgt agaatctagc ggaaatttct tagcactagg caatgcaact aaaactgaaa
     1021 ttaccggaaa acacttacgt ggaggtgtat tatgtgatgg tttaggctca gcatttgcgg
     1081 caattatgtc cacaactccc ttttcttctt tctcacaaaa tattggggtc atttcattaa
     1141 ccggtgtagc cagtcgctat gttgtagcat ttacaggcgc attacttgta ctagccggtt
     1201 tatttcctgt atttggagca ttaatcgtgt ctatcccgtt acctgtgctt ggcggtgcag
     1261 gcttaatgat gtttgccatg attatcgcag ccggtatcca aatgttagat aatattcaac
     1321 gtagcaaacg aaacggttta attattgcta tctctatcgg ttgtgggctg gccgtaacaa
     1381 ctcgacctga gctattggat aagcttccac acttctttaa agaagttttt ggctcaggta
     1441 ttacggtcgg ctccttatta gcacttattt tgaatttagt tttacctgaa gataaaataa
     1501 ccaagaaaga aaataagagt gatgaataaa tcaccctaaa aaaacaagga cagctcacaa
     1561 tacaactttg atgctgtcct ttattcccat agagagcata tttaaattta ggttaataaa
     1621 aaaatctgca ctctatattg gtgtttacct ttagagtaca gatttcttct tattctaact
     1681 ttctagcaaa caattagatt aatgttccgc tttggtatcg gctaacgaat ccccaatgtt
     1741 attatcttta tttggattat cctctaaagt tgaacttaca tccgctgcct gttcaccact
     1801 tggtggagtg gcgttatcgt ctttagttgg ggtatctgtc caacctgatg gttcacccac
     1861 cggacggcgt gccattaaat catcaatttg cttgctgtct aaggtttcgt atttcaataa
     1921 ggcatctttc attgcgtgta agatgtccat attttcttcc aaagtttgct tcgctcgttg
     1981 atagttgcgg tcaatgatct tacgcatttc cacatcaatc aatttttgcg actcttccga
     2041 tacgccctta attgcaccca ttccatcttc attttgatag aaaatcgggc ctaattcttt
     2101 tgagaagccc cattgagtaa ccattgctcg tgcaatttgg gttgctcgat gaatatcaga
     2161 ggaagcccct gtggtaattt tatcttcacc aaaaatcaag ccctcagcaa ttcgccctgc
     2221 aaatagggtg gaaagttggc tttccaattt ggtaaatgtt tcgcttacac ggtcaccttc
     2281 cggtaagaat tgagcaaaac ctaacgcctg accacgaggc acaattgtca ctttattcag
     2341 cggatcgtgt tccggcatta aatagccaac aataacgtgt ccagcttcgt ggtaagcagt
     2401 attgatgatc tctttttccg tcattgtatt tgaacggcgt tccggcccca tattgatttt
     2461 gtcacgggct tcttcgaaat cttccatcat taccacacgt ttattcttac gggcagcaaa
     2521 taaggcagct tcgttcacca agtttgctaa ttgagcacca gagtaacccg gcgtaccacg
     2581 agcaatttgc atcgggtcaa ctccctctgc aagcggtact tttttcaagt gaactttcaa
     2641 aatttgctca cgccctttca cgttcggcaa atcgaccgtg acttggcggt cgaaacgccc
     2701 cggacgagtt aaggcatcat ctaacacatc ggcacggttg gttgcagcga tgataatcac
     2761 accttcagag ccttcaaagc cgtccatctc aactaacatt tggttgaggg tttgttcacg
     2821 ctcatcgtgt ccaccgctga aacctgcacc accacgttta cgccctaccg catcaatttc
     2881 atcaataaaa ataatacaag gggcattttt cttggcttgg tcgaataaat cacgcacccg
     2941 tgaagcaccc acacctacaa acatttccac gaagtcggaa cctgccattg taaagaatgg
     3001 cactcgtgct tcacctgcaa tcgctttcgc caataaggtt ttacccgtac ccggagggcc
     3061 taccatcaag atcccttttg gaatgcgccc acctaatttt tgaaatttag ttgggtcacg
     3121 caaaaactca acaacttcgc ctacttcttc tttggcttca tcacagccgg ctacatcaga
     3181 aaatctggtc ttaacttcgt ctgcggtcaa cattttggct ttatttttcc caaagcccat
     3241 tgcaccacgt cctccgccac cttgcatttg tcgcatataa aacacataga aaccgattaa
     3301 cagcaacatt gggaaccaag aaatcaaaat ttgcgagagc aagccacgac gctcagcagg
     3361 ctgaccggta atcactgcat tggtttttgc taaatctgcc attaagtact catcatacat
     3421 cggcattacg gtttggtagg ttgcaccatc atttttcgtc accaaaatgg tgtcatcatt
     3481 ctgtttgaaa tcgacccctt ttaaacgatt ttctttaata tcgcttaaaa aagtcgaata
     3541 tggcactgta tttgagctac cactgaaatt agaattaaaa ccttcatagg ctgtcatcat
     3601 cacaatggca atcaccaccc aaagtaaaat atttttaacc atatcgttca aggttaatcc
     3661 cctatctgct gaataaaaaa ctgttcaaga ttactataaa agcggtcgaa ttttccaatt
     3721 tttttgcaaa atgttaataa aaaatgaccg cttgcttgtt ttctagcctt tataacctgt
     3781 cgctacaata taaacttctc gagaccgatc tcgagacgcc tccggttttc tgacttttac
     3841 cacactaaag agcgagcgga tttctcgaag gtattcatca aatccttcgc cttggaatac
     3901 cttaaccaca aaactgcctt tggttgctaa tacttggcga cacatatcca atgccaattc
     3961 cactaaatac attgcacgag gaatatcaac cgacggcata ccgctgaaat tgggagccat
     4021 atccgacatc accacattca ctttgtcttc gcctactcgc tcaagtaacg cattcagcac
     4081 cgattcttca cggaagtcgc cttgtaagaa atccacgcct acaatcgggt tcatatctaa
     4141 aatatcacaa gcgattactc tgcccttact gccgatttgc gtcaccacat attgcgacca
     4201 accgcccggt gcggcaccga gatccaccac cgtcattccc ggtttgaata aacggtcggt
     4261 ttgttgaatt tcatctaact taaaatacgc ccgtgaacgc aatttttgtt tatgtgcttt
     4321 ttgcacaaat tgatctttaa aatgttctgc aagccaacgt gatgaactcg cacttcgttt
     4381 tctacccatt tgttcttcta tcttaattaa ttcaacgatt tataataagg gttaaattag
     4441 caaattcaag agatgttcta aaatttagct cggcttatta tgcttaatta a
//
LOCUS       DANLUA010000187         4441 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLUA010000187 DANLUA010000000
VERSION     DANLUA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            155..1189
                     /locus_tag="PXB44_002263"
     CDS             155..1189
                     /locus_tag="PXB44_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282696.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            1257..3187
                     /locus_tag="PXB44_002264"
                     /pseudo
     CDS             1257..3187
                     /locus_tag="PXB44_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            3285..4334
                     /gene="fbpC"
                     /locus_tag="PXB44_002265"
     CDS             3285..4334
                     /gene="fbpC"
                     /locus_tag="PXB44_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL5282697.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGEYLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         1190 a          969 c          985 g         1297 t
ORIGIN      
        1 ttagaaaggt tcaactacta ccacctatga attgcaaatt ttttcacaat ttaggccgct
       61 tgttgattga actgtgagct tgctcacaga attttatggt atttcttgct atattgtata
      121 gcaatctatg cgataactca ttaaggaaat aaaaatgaaa ttaaaaaaat tatctttagc
      181 actctcgact ttattactcg gttcagcctt tgccaactct gctttagcgg aaggtcgttt
      241 aaccgtttat tgtagtgcca ctaatgaact ttgtgagcaa gaagtgcaag cattcggcaa
      301 aaaatataat gttaaagtag catttgtgcg taatggttca ggcagtacat tggcgaaaat
      361 tgaagcggag aaaaacaatc ctcaagctga tgtatggtat ggcggtactt tagatccaca
      421 ttcacaagct gcagaaatgg gtttactcga gccgtataaa tcaccgaatc tcgaacaaat
      481 tatcgaaaaa ttccgtgatc cggcaaaatt aaaaggcaac tactcttcgg ctgtgtatat
      541 ggggatttta ggttatggtg tgaatttaga ccgaatcaaa aaactcggca ttgaaaaagt
      601 gcctagcact tgggaagact tgctcgaccc acgcctagcc ggtgaaatcc aaattgccga
      661 cccacaaagc tccggcacgg cttatactgc gattgcgacc ttcgtacaac ttttaggaga
      721 agaaaaagcc ttcgactatt tccgcaaatt acacaaaaac atttcacaat ataccaaatc
      781 cggcatcaca ccggcacgca ataccgcccg tggtgaaacc gctatcggta tcggtttctt
      841 acacgactat gcgattgaga agaaaaacgg ggctaacatt gaaatgaccg caccaacaga
      901 cggcacaggc tacgaattag gtggtgtgag tatcctcaaa ggtgcaagaa atcttgataa
      961 tgcaaaactg ttcgtggatt ggtcgctttc taaagaagct caagaactct cttggcaaaa
     1021 aggcaatgcg ttccaagtat taaccaacac caccgcagaa tcttcccctt atgctctaga
     1081 tccgaaaaac ttaaacttaa tcaactatga tttcgagaaa tacggctcga gcgaagagcg
     1141 taaacgcttg attgataagt gggtaaatga agtaaaatta gcgaaatagt gttaatcttt
     1201 ccactgaggg gataagcaat tatcccctct ctaattataa gaatctgtga ggtgttatgc
     1261 agaatttttc cttttcccga ccgcttgttt gggtgcttat cgggttactc ggttttctct
     1321 tgttaccgtc taaagcctta gattatgggc tgtttgactc aacctctgat gaactgctcg
     1381 ccgcaatggg ctgggcgtgc tgttcgcctt tattttcctg tcagcgattg tgctgaaaat
     1441 cagcctcggt tatgcggtgt tgccgttgat tattgccatt atggggcttg cgaccccaaa
     1501 tttagcaacg ctcaaagtaa tgcagggtga ccgctttatt atcggctcgt tactgagcat
     1561 tattctgctg attttctttt ttattgtgta tccgaccatc gccatttttg tgtcgatgtt
     1621 ctacaacggc tcggaatttg tgccaagcca agtgctgaca attttgcaac aaccttatgt
     1681 cgttcgagtc atcggtaact cgctctcggt tgccggcacg gtgggcgttc tctccaccct
     1741 attcggctta gcctttgctc tctacaccac tcggattgct aaacgctcgg cgtttatcgg
     1801 gcggattttc tcgattttac ctatcgtcac cccgccgttt gtggtcggtt taggcgtaac
     1861 cctgatgctt gggcgttccg gctatgttac tgaattttta gtggattact ttggctttgc
     1921 caacaattgg ctgtacggtt tcaccggtat tgtgatagcc cataccctcg cgctcacgcc
     1981 aatgtcgttt atgattttgg aaggggcatt gaaatccatt cacccatcgg tggaagaagc
     2041 agcttacacc ctgcgttcca accgctatca agcctttttt aacattattt tcccattgtt
     2101 aaaaccggca ttggcaaact cctttttagt ggtggcaatt caatccctcg ccgatttcag
     2161 tacgccgtta gtattgggcg gtagctttga tgtgattgca acccaaatct acttctacat
     2221 tgccggttct caactggatt atgcctctgc tagcacgctt ggggcaattt tgttggtgtt
     2281 ctccttagcc attttcgtga tccaatacct ctggatcggc aatcgctctt atgtgacggt
     2341 gtccggcaaa tcctatcggg gtgatgtcca agacttacca agcggtatga aatggctgat
     2401 tatttgcacc ttattgttct ggggaacctt caacttagtg ctgtacggca gtattttcta
     2461 cggcagtttc accgtgaact ggggtgtgga ttatacctta acccttgata actacatcaa
     2521 gctattcggg caagggttta gcgatggcgg tttcccttcg ttaattcaaa ccgtgatttt
     2581 cgctgccgct gccgccccga ttactgcact attcggcttg ctgatcgcct atgtgacagt
     2641 tcggcgtgat ttcaaaggca agaaaaccct ggaattttta accctgctct gctttgccgt
     2701 accgggaacg gtggcaggtg tttcttacat tctcgccttt aacgatgccc cgatttacct
     2761 aaccggcacc ggtatgatca tcattctctc aatggtaatg cggaatatgc ctgtgggtat
     2821 gcgagcggca attgccggct tagggcagtt ggataaatcg ctggacgaag cctcgctctc
     2881 gctgaaagcc ggttcgttca aaacgatgct gtttgtggtg ttaccgctac tcaaaccggc
     2941 actgctctcc gccctcgtca ccagctttgt gcgggcaatg acgactgtca gtgcgattgt
     3001 attcttagtc accgccgaca ctcgtgtggc aacctcctac attctcaacc gtgtggaaga
     3061 cggtgaatac ggcattgcga ttgcctacgg ctcaatttta attgtggtga tgatggcgat
     3121 tatcctgatt tttgactgga ttgtcggcga cacccgtatc gcccgttcta aggcgaaaaa
     3181 aatgtaaaaa gacatctact ctctcccttt atgggagaga gacagaaaaa tacttcgttc
     3241 agaagtattt ttcagagaga gggatataat taaaaggaca atatatgaac aacgatttct
     3301 tagtattaaa aaacgtcacc aaatctttcg gcaaggtgac ggtcattgat aatctcaacc
     3361 tcagtattcc gcaaggtaaa atggtgacat tactcgggcc gtccggctgt ggtaaaacca
     3421 ccgtactgcg tttagtggca gggctggaaa acccgacttc ggggcaaatt tttattgatg
     3481 gcgaagatgt tacccaaagt tcgatccaac accgtgatat ttgcattgtg ttccaatctt
     3541 atgcgttatt tccgcatatg tcgattggcg ataatgtcgg ctacggcttg aaaatgcagg
     3601 gcgtaccgaa agaagaacgg ctgcaacgag tgaaagaggc gttggaattg gtggatttag
     3661 gcggctttga agatcgcttt gtcgatcaaa tctctggcgg acaacaacaa cgtgtcgccc
     3721 tcgcccgtgc cttaattctg aaaccgaaag tgctactgtt cgatgaaccg ctgagtaacc
     3781 ttgacgctaa cttacgccgt tcaatgcggg agaaaatccg tgaattacag caacgtttaa
     3841 acattacttc cctttatgtg actcacgatc agtccgaagc ctttgcggtt tcagatgaag
     3901 taattgtgat gaacaaggga aaaattatgc aaaaagctcc agccaaagag ttataccttc
     3961 gccctaattc tttattctta gcaaatttta tgggtgaaag cagcattttt gaaggcacga
     4021 tagctggcaa ccaaattaat ataaatggat atcaatttac gttagcaaat gctgaacaat
     4081 ttgagttacc cgatggggaa tacttagttg gcattcgtcc agaagctatt catttagctg
     4141 aacaaggaaa tataagtcaa ctctgtaaaa ttaaaaatgc agtttatatg ggaaatcatt
     4201 gggaaattat agctgaatgg gaagggaaag atttattagt caatactaaa cctgaaaatt
     4261 tttaccctca tatcaaacag gcctatgtac attttatcga acaaggtgtt tttcttttaa
     4321 agaaagagct ataacgaatt tatactaaat taagaggaag gtagttataa ctatttagca
     4381 attttgagaa aaataggccg catgcaaaat ctcttgatga caagcggtct tttctcgccg
     4441 a
//
LOCUS       DANLUA010000188         4421 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLUA010000188 DANLUA010000000
VERSION     DANLUA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(232..999)
                     /locus_tag="PXB44_002266"
     CDS             complement(232..999)
                     /locus_tag="PXB44_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5282698.1"
                     /translation="MSKIIKIGKVANNSDAIDAISTQPRHSVIVGTIKRLQTDRIDLL
                     YQHRVDPNVPIEEVAGAIKDLMKQGKVLNWGLSEAGEKTIRKAHAIQPLTAVQSEYSL
                     FFRGREKDVILVCEELGIGFVPWSPLAMGALAGYVDENSRFEADPSKDLRGIIPRFTP
                     EAMQQNVKLIQLVQKWAKRKLCTPAQLSLAWLMAQKAFIAPIPGTSKARHLLDNLGAD
                     DVKFTEEELAQFRQELEAIEIVGLRLPEAILNFSEAK"
     gene            998..1318
                     /locus_tag="PXB44_002267"
     CDS             998..1318
                     /locus_tag="PXB44_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282699.1"
                     /translation="MELKVIHKNLVDSAKIKLHAVYLVIFLFVSHFAMIFGMADPISA
                     GYQPTTPAMTHSMQMDAKQITEMHNQMMTGEITPEEMMKMHQEMNPNMNMPNTQGGHS
                     EHHK"
     gene            1500..1955
                     /locus_tag="PXB44_002268"
     CDS             1500..1955
                     /locus_tag="PXB44_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL5282700.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            1969..3516
                     /locus_tag="PXB44_002269"
     CDS             1969..3516
                     /locus_tag="PXB44_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL5282701.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(3606..3761)
                     /locus_tag="PXB44_002270"
     CDS             complement(3606..3761)
                     /locus_tag="PXB44_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5282702.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            3850..4029
                     /locus_tag="PXB44_002271"
     CDS             3850..4029
                     /locus_tag="PXB44_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282703.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            4078..>4421
                     /locus_tag="PXB44_002272"
     CDS             4078..>4421
                     /locus_tag="PXB44_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282704.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGT"
BASE COUNT         1358 a          915 c          890 g         1258 t
ORIGIN      
        1 aaattcattg agttgattat gccattttta cacttattga ctatagcgtt ttgcctaatc
       61 atctgtctga ttccagtttt acccttttcc caatatcttt caatttcgat aaatcttcca
      121 ctaatcttgc tacatattca agaaaaagtg cgatcgtttt tcaaaggatt ttatgaaaac
      181 gaccgctgta agaaagaatt tttatttacg actttttcag gtatctgaaa actatttcgc
      241 ttctgaaaaa ttcaaaattg cttcaggcaa acgcaagccg acaatttcaa tggcttctaa
      301 ttcttggcgg aattgcgcca attcttcttc ggtaaatttc acatcgtctg cgcctaaatt
      361 atccaacaaa tgacgggctt tgctcgtccc aggaattggc gcgataaacg ctttctgtgc
      421 cattagccag gccaaagaaa gctgtgcagg cgtacaaagt ttgcgttttg cccatttttg
      481 cacaagctgg attaacttca cattttgttg catcgcttcg ggtgtaaagc gtgggataat
      541 accacgcaaa tctttgcttg gatcagcctc aaaacggctg ttctcgtcca cataaccagc
      601 caatgctccc attgccagcg gcgaccaagg cacaaaccca atccctaatt cttcacaaac
      661 gagaatgaca tctttttcac gaccacggaa aaacaacgag tattcgcttt gtacggcggt
      721 taaaggctga atagcgtgtg ccttgcgaat ggttttctcg cctgcttccg acaagcccca
      781 attcaaaact ttgccttgtt tcattaaatc tttaatagcg ccagccacct cttcaatcgg
      841 cacattggga tcaacacggt gttgatagag taagtcaata cggtcggttt gtaagcgttt
      901 tatcgtaccg actattactg aatggcgtgg ttgggtagat atcgcatcaa ttgcatcact
      961 attattagct acctttccta tttttattat tttactcatg gaattaaaag ttattcataa
     1021 aaatctcgtg gatagtgcaa aaattaaatt acatgcggtt tatcttgtga ttttcttatt
     1081 tgtgagtcac tttgctatga tctttggcat ggcagatcct atttcagcag gctatcaacc
     1141 cactacacca gcaatgactc attcaatgca aatggatgct aaacaaataa cagaaatgca
     1201 caaccaaatg atgacgggcg aaataacgcc tgaagaaatg atgaagatgc atcaagaaat
     1261 gaaccctaat atgaatatgc cgaatactca aggtggtcat tcagaacatc ataaataata
     1321 attctgatca cattttaaaa aataatagtg gatggtacgt tggcatatct ctactacaag
     1381 cagtaggatt ttgagaaaaa ttttcaaatg atagaatttt ttcagattac ttgaccttaa
     1441 ccctaggtta agctttaaaa tgatgtccat tcaatctcaa taaaaaataa ggacatctta
     1501 tgaaattaca taaattcacc cgctcattgt tgcttttaag tttaggcata accgcttttg
     1561 ctcaagcaca aacactttct atggaagtgt ggaaaagccc gacttgtggc tgttgtaatg
     1621 aatggatcag ctatatgcag aaaaacggtt ttgaggttaa agtcaacgaa acaggcaatt
     1681 atgatgctca taagcgcttt aacattaaat atgaacatgc ttcttgccac acagccgtga
     1741 ttgatggcta tgtgattgaa ggacatgtac ctgctgagga tatcaaacgt ttactcgcag
     1801 aaaaacccga tgcaataggc ttgtctgcac cgggaatgcc aattggttca ccaggtatgg
     1861 acggtgaagc atacggcgga cgcaaagatc cttatgatgt tgttttgatt aagaaaaacg
     1921 gtgaaagtga agtgtttaaa tcctataacc aatagggggg gggaaagaat gaaacgtcga
     1981 gattttttac gttatggaat tggaattagc ttagccagta gctcgcttta tagcctcgcg
     2041 aatatgatga accacgcaca acatggcaat atggcgatga tgcattcagt acctactggc
     2101 ctaatgccaa ctgaggcaat gccttcaggt ttatcgctta atgggatatt accaaaactt
     2161 gctaatcaat cgactcaagc tggactgttc aaagccactt taaaagcaga gccaattaaa
     2221 attcgcctcg cagacaataa agaaacggaa ttttgggctt ataacggaca actaccaggg
     2281 ccacaaattg aagtatttga aggagatacc gtagaaattg aatttatcaa tcacctacca
     2341 caaccctcaa cagtacattg gcacggctta gatgtaccaa atgaggcgga tggtaacccg
     2401 atggatatgg ttgaaccgca agggaaaaaa gtctatcgct ttacgctacc tcaaggcagt
     2461 gctgggacat attggtacca ccctcatcca catgaccatg tttccgaaca agtctataaa
     2521 ggcttagcag gcactttcgt tgttaaagcg aaaaatgatc cgcttgcaca ccttcctgaa
     2581 caacattggg tgatttccga tctgcgttta aatgccgatg gcaccattcc agcaaacacg
     2641 atgttagatt ggataaacgg tcgtgagggt gaatttgtgt taatcaacgg tcaatatcag
     2701 ccccagattc aagtgaaaac gaatgaacga atccgtcttt ggaatgccac tagcgctcgt
     2761 tattttcgtt taaacattcc aggggtgaaa tggattgtgg tgggtaccga aggtggttta
     2821 cttgaaaaac ctcgctcgcc tgttgatgaa ctgctactta caccagccga acgcgttgaa
     2881 gtgattatgg tgggtgaaac gcaaggaaca gtcaatttac aaagcggtta ttatgatcgc
     2941 cgtaagatga tggttcaaga acagcctaaa gatctcactt tagccacaat tcaggtgaaa
     3001 tcagaacctg ttcagttacc tgaaagctta aggtccttgc ctaactggga tgagccaaag
     3061 caggttcgcc aaattcgctt tagtgaaaaa atgatgaacc atacgaatat gccaatgatg
     3121 aatcacggta cacatcacgc tactacaaca ctaacggaca accctattcc accaatgatg
     3181 aatggtatgt tcttaatcaa cggtcaaacc tttgatatga accgtattga ttttgttacc
     3241 aaattgaatg aagtggaaca gtgggaaatc ttcaatgaaa gccatatgga tcatccattc
     3301 catttacacg gcactcaatt tgaagtaatt caacatacgt taaacggtaa aacctttaag
     3361 ccagaaggca gagcgttaaa agatgtggtt aatttacgcc cttatgaaaa agtgattatt
     3421 cgctttaagc aaggacatac tgggttgaaa atgtaccatt gccatatttt agaacatgaa
     3481 aatctcggta tgatggggat gtttaaagtg gaatagtggt atctaactat aaaaatagag
     3541 ggatttcgtc ataaaaaatc tgcaccttaa tcagttggtt tttagtccaa cttttggggt
     3601 gcagatcaga cgctcacttt gtcgccgatt tgaaattcgc cagcgtcatc gcccactgcc
     3661 acgaccacgc ctgcccctgc ataacccaaa gtcgcaggga acacagggtc aatcacatac
     3721 gccccttccc gatacatcat ctcggcacgg tttagtccca atgcacggca cggtttttaa
     3781 acattttcat tatagttttc cttatcaaaa tgggatttgg caaacattgg gcaaacctaa
     3841 gcccgcccaa tgtttttagt taatcaatta cttgcaatca caatcgccac aagtgcagtt
     3901 ttgacaaatg cacagctcgc cattttggca gttgcatttt ggattgcaat tttgccctgt
     3961 gcaagattgg caaccgcagt gggcgtttgg gtttttgatg atgtcgttca taacaattct
     4021 ccaaattaaa ggttgattat caaaacaaaa accgttttta aacccattaa taaaaaaatg
     4081 ccatttaaaa cggtcacttt tgcacaagca acaaccaatg atggtcaatc cttattagcg
     4141 gttaatgact tatttattgg ccctaaaagc cacacttccg cacagtatat tttgcaatgg
     4201 aatggcgctg aagaagtgca atcttcatca ggcattattg tatcaacagg attgggatca
     4261 acggggtggt ttcaatctat tcttgctggt gcgatggcaa ttacaggaga agcttcgcac
     4321 cctctattac aaggctttag ctggagtgat cgaaagctac aatttagtgt aagagagcca
     4381 tttccaagta gaacaacagg tgttgcactg acttttggca c
//
LOCUS       DANLUA010000189         4412 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLUA010000189 DANLUA010000000
VERSION     DANLUA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1150
                     /locus_tag="PXB44_002273"
     CDS             <1..1150
                     /locus_tag="PXB44_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL5282705.1"
                     /translation="SYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDDFVYH
                     KNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATDNYAL
                     VEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGDQFHS
                     DNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGIDNEE
                     LIQYLSRVSLSSVTQGTSQIGYHAVKLLYRKLNNLPVSTKPILIPPVKVEERRSTDYR
                     SLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVIHEEK
                     LARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            1243..2242
                     /gene="gntR"
                     /locus_tag="PXB44_002274"
                     /pseudo
     CDS             1243..2242
                     /gene="gntR"
                     /locus_tag="PXB44_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
     gene            complement(2296..2811)
                     /locus_tag="PXB44_002275"
     CDS             complement(2296..2811)
                     /locus_tag="PXB44_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL5282706.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            2998..4347
                     /locus_tag="PXB44_002276"
     CDS             2998..4347
                     /locus_tag="PXB44_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5282707.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
BASE COUNT         1158 a         1020 c          989 g         1245 t
ORIGIN      
        1 atcttataaa attgccttac tttttaatgc taacaaaatt tatgacagag gagtaatgca
       61 gggcattggg cagtttattc aagcatcaca atgtacttgg gatatttttg tggaagatga
      121 ttttgtctat cataaaaata gtatcgaaag cctgtcgatt gacggcatta ttgccgattt
      181 tgacgatttg gaaacagttg agctacttaa agatattgac gtccccattg tagcagtagg
      241 cggttcttac caaaaccctg actattaccc tgaccttcct tatgttgcta ccgacaatta
      301 cgccttagtt gaggcagcat ttttacacct aaaacaaaaa ggaatcaacc aatttgcgtt
      361 ttacggctat cctgcagaaa cggaaaaaca ttgggcgata gagcgaaaaa atgcctttat
      421 tcagttgatg gaaaaatatg aacatcaccc aaattgttat attggtgatc aatttcattc
      481 agataattgg gttgaattac aagccaaatt aggcgagtgg ttacaaacct taccagccca
      541 cacgggcatt attgccgtta ctgacgcaag ggcaagacac ctgcttcaaa cctgtgagca
      601 tttaaaaatc gctgtacctg aacaactttg cgtgatcggc attgataacg aagagttgat
      661 tcaatacctc tctcgggttt ctctttcttc agtcacacaa ggaacatcac aaatcggcta
      721 tcacgctgtg aagttattat atcgaaaatt aaataatcta ccggtttcaa ccaagccgat
      781 tttaatcccg ccggtaaaag tggaagaacg ccgttcgacc gattatcgtt ctttgcaaga
      841 cccaatggta atccaagcta tgcactttat tcgtcaccga gcttgccaag gcattaaagt
      901 agagcaagta ctggatcatc ttaaaatttc ccgctctaac cttgaacagc gttttaagca
      961 ggaaatgaat aagacaattc accaggtgat tcacgaggaa aaattagcaa gagcaacata
     1021 tatgctcaaa tttaccgaga tttcgaccca agaaattgcc aatgtgtgcg gttatccctc
     1081 tttacaatat ttctatgccg tgtttaaaaa agagtttggt aaaaccccga aagagttcag
     1141 gcaagagtag taacaagcgg tcaatttcac cgaatatttt gcaattattg ccttatcttc
     1201 agttataatc agcgaaatta aattttttcg ttatctattc caatgagaaa accgaaacgc
     1261 ccaactttgc aagacatcgc tacccatctc ggcattacca aaatgacggt cagtcgcttt
     1321 ttgcgggatc caaacaccgt tgccaaggag accggcgagc ggattgcaca agcgattgag
     1381 ctgtttggct atatcccaaa ccgtgcaccg gatattctct cgaatgccaa aagccgtgca
     1441 attggcgtgc tggttccttc gcttaccaat caagtgtttg ccgatgtgat taaaggcatt
     1501 gagcagatta ccgaccaagc cagctatcaa accatgctcg cccactatgg ttacagcgaa
     1561 caaaaggaag agcagcggat tgagtcgctg ctttcgtaca atattgacgg cattatctta
     1621 tcggaaaatc accactcgcc ccgcaccttg aaaatgctgg aagtggcgaa tattccggtg
     1681 attgagataa tggaaagcgg cgaatgtggc attcaacaag cggtcggata ttgacaatat
     1741 ttctgcggct caagcaatgg tcgagacaat gattcatcga ggctgccgac aggtggtgta
     1801 tttcggggcg agaatggata aacgctctca gctgaaaatg cagggctacg aacaggcaat
     1861 gctcaaacac ggcttaagcc cacacagcat tactactgca gagcattctt ctttcacgct
     1921 tggcgcaagc cagctaaaac aagccctgca acacattccg cagctagacg gcattttttg
     1981 caccaatgac gatttggcaa tcggggcatt atttgagtgc caacggttag gcattcatgt
     2041 gccgcagcag attaaaatcg ccggctttca cggacacgat gtcgggcaat ccctcacgcc
     2101 acagcttgcc agcgtcatca cacctcgcct tgaaataggc agagttgccg cccaagagct
     2161 gctcaaccgt attcacggcg taccacaaca aagctccata attaacttgg gctatcagat
     2221 ccatttgggg gaaagtgtgt agcccggaca agcggttaaa tttgcaaaaa aatccgcaaa
     2281 atccaccact tgtgattaaa gcaacggctc aactgctttc acgcaacgct ccaccacctc
     2341 gtcaaaactg ccgtcaatat caataaatat cacatctggt tcgtctgcct gcggaatttc
     2401 cagcgtatta aattggcttt gaagcatttc cggtttcata aaatgccctt tgcgttgttg
     2461 cattcgagcc agcaccagtt caaacgagcc gtgcaagaat agaaaagtca catcggcatt
     2521 gccttgtcgg atctggtctc ggtacttttt cttcaatgct gagcagacga taatcccttt
     2581 ttcgcttttt tgttcaaaac tgaacactgc atcgttaatc cgctccaacc acggtgtgcg
     2641 atctgcatca ttgagtggcg tgccacttgc cattttcagt atattggcac gagggtgtaa
     2701 gtcgtcccca tcaatcaatt ttattcccaa ccgctgtgcg actgccgttc ccacgctggt
     2761 tttgccggtg ctggacacgc ccatcaaaat aaatgctttg ccgtttgcca ttgaatttcc
     2821 ttgtgttact ggtaacgtta acttagccgt taatgtaaca ggttatgctg aaaaatcatc
     2881 tgctatttcc agaaaacttg aagtagatca catttttaaa ataaaagccc tgcaaccgtt
     2941 tactctaaac tgctttttgg ttatgttacc gataacaatt caaccttagg agatcctatg
     3001 ttactgttca ttatgtttgc tgccatcatt ttattgctgg tgctgattat gaaatttaaa
     3061 gtccacgctt ttgtggcatt gattattgtg agcctgctca ctgcattagc tgccggtatt
     3121 ccggtagata aaatcctgcc aacattgttg aatggttttg gtagtacgct ggcatcagtg
     3181 gcgttactgg tggggcttgg ggcgatgatt ggtcgcttgc ttgaaatcac cggcggggcg
     3241 aaagtgcttg cggatacgct tatcaataaa ttcggcaaag aacgtgcgcc ttttgcgttg
     3301 ggcgttgcct cgctgttatt cggcttcccg attttctttg atgccggctt ggtggtgatg
     3361 ttaccgatta tttttagcgt ggcaaaacag ttcggcggtt cggtgttgcg ctatgcgttt
     3421 ccgtctgccg gtgcgtttgc ggtaatgcac gcctttctac ctccgcaccc cggcccggtt
     3481 gcctccggcg atctgcttgg tgtgaatatg gggctgttag tcttggtggg cttggtttgt
     3541 gcaatcccaa cgtggtatat cggcacttac ctgttcagcc aatttatcag caaacgcatt
     3601 cacgttgatt tgccgaaagc gtttttaaac ggcttatcgg caaacgaaat ggcagtacaa
     3661 aacccgccaa gttttggcaa ggtactcacg gtgttggtgc tgccgattat cctgattttg
     3721 tttgataccg gcttgaatac cctctccgtt gctaaagtga ttgatggcag tgaaatgtgg
     3781 gtgcagagcc ttcgcttaat cggcaaaacc cctgttgctt tactgattac cttaattgtg
     3841 gcgattatgc tgttgcggga taatcgcagt ttcgaccaaa tcgaaaaaat ctgtaacaat
     3901 gcccttggcc caatctgctc gattgtctta gtaaccggtg caggcggaat gtttggtggc
     3961 gtgttgcgtg ccagcgggat tggcgatgta ttatcggaaa tgatgaaaga caccggaatg
     4021 ccgattgtgg ttgccgcctt tattattgct acggtattcc gtgtcgcaca aggctcggca
     4081 accgttgctt taaccaccac agcagcgttg attgcgccaa ctgtggcggc agcgactgat
     4141 ttaagccagt tcgacctctg ttttatcgtg attgctatcg cctccggtgc gacggtgctt
     4201 tcccacgtca atgactccgg tttctggctg atgagccgtt tccttgaaat ggacgaaaaa
     4261 actaccctca aaacgtggac aatgcttgaa actgccatcg gtttaaccgg ttttgcctgt
     4321 gcattgattg gcagctggtt actctaattc caaccctgac aagcggtctg ttttcttcta
     4381 aaatttgcaa aatttggcag agaatcgacc gc
//
LOCUS       DANLUA010000190         4400 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLUA010000190 DANLUA010000000
VERSION     DANLUA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1246)
                     /locus_tag="PXB44_002277"
     CDS             complement(<1..1246)
                     /locus_tag="PXB44_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL5282708.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTA"
     gene            complement(1265..2575)
                     /locus_tag="PXB44_002278"
     CDS             complement(1265..2575)
                     /locus_tag="PXB44_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL5282709.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(2587..3351)
                     /locus_tag="PXB44_002279"
     CDS             complement(2587..3351)
                     /locus_tag="PXB44_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL5282710.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(3351..4286)
                     /gene="hemC"
                     /locus_tag="PXB44_002280"
     CDS             complement(3351..4286)
                     /gene="hemC"
                     /locus_tag="PXB44_002280"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL5282711.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         1230 a         1021 c          708 g         1441 t
ORIGIN      
        1 tggctgtttg cggtaaggct aatggctcat taactttaga ctgagtagcc atattcacct
       61 gtttgagatt ttcctcacga atacggtttg ccattaatgt atcccccatc tgctctgcca
      121 cgtgcagtgc cataatacga tcatcagcaa cacattcatc atgagccaat aacaattcaa
      181 actgagactt cgctttttgg tattgcccat ctcgtgtata aatatagcca agcgctctag
      241 cataatcatc ggtatattta acattcgctt tatctgcccg ttttgctaat acttttagta
      301 gcttactgtc agaatcaatt tgtaaacggg ttaattgcac aaataaacct tgtaattgct
      361 catcttcaaa tttcttcaac gtttctaaag caatttcttg tgcagattga tgatcgtcac
      421 tatcaattaa acgcttgatg acaccgacac gactatacac cgatttacga cgacgatttg
      481 gttggttttc ccaccaagaa agcaagcctt cctgcccttc ttcctgtaaa cgctcatctt
      541 gtaaaccatc atcaacgaaa tactctaact cagcatactc ttctgcagtt aagaaattac
      601 gttggccgat ttcaactaat aatctatcta acgcaacata agctctggat tctttataaa
      661 tttgaatcgc taagcgtaat acctcatcgc tatgaggtgc aagctctagt aggctatcaa
      721 tcgcagttcg ggcagccggt aattttcctt gttgtaataa aattcgggtt cgtaccaact
      781 ccactgcaac attattcggt ccagctattt cagttgcctt aattaaatat ttatttgcag
      841 ctaaatcatc accattttgc tgagcagctt ccgccgcttt aattaaattc agtacaggct
      901 catctgcatg ttttgcattt ttactaaaga gtttttccgc ttttgagtaa ttcccttcac
      961 tcatacgcat taaaccttcc agtgtttctt gctgagcttt tttatgctta cgagttgaga
     1021 accagttata agctccatta ctcatacggc atattttagt gacaatccat tcaagtaaat
     1081 aaactaccgc cattgccaac acaaaaaaaa ccacaagcat tacaatgctc atttcaatca
     1141 cggtagttgc tgtttcaatt ctgacataac cttgatttcc ggctaaataa ggcccagaga
     1201 ttagtccaac taataataaa agcatcaaaa agagcgttct aaacataatc tattctcctt
     1261 tttattattg tgctgaaaga ggcatcgcat cttgctctgc ttttacaggc tctgcttgct
     1321 ctaattcttt ttcagctttt atttcgactt tctctatttt ttcaggggtt ttattaagtt
     1381 gctgagcaat cacctttaag ctttgtaatg aagttggagc atccacataa atagtttgtt
     1441 ctgccaactc atctagctct ttcaagaaat gttgcacatt ttcatttgaa gtttcaaaat
     1501 aacttcttac ccaagagcct accgtttgta acgatttttt ataaagttcg ctttgctggc
     1561 gaggaacagc taaaatcgca atttgtaaac gcaagcgaat gttttcacgc aaataaattt
     1621 cttgattcgg tgcaacaaag gctttctcat ctgctgcatt acgtttcgat acacgaataa
     1681 aatgatctaa aaatgaagca gcactttttt ctaaattttt ctcccaatcc gcaagagaat
     1741 cacttacttg cccatcttgt ttgctatttg aagcttgttc actttccaaa atcggcaaat
     1801 catctaattt attcgctaaa tttgcgacac gctgcattaa ggcattttga tcgatctgat
     1861 gcaatgcggt taatgtattt aaatcagttt tgattgcctc acgaatgtga atcgtattcg
     1921 gtgcttttga taaaacttga tctgcctcta acaataaatt tttcgctgtt tcaatgtcat
     1981 tatctaacac cactttacga gccgcattat tcaataggaa atctgcctca gataatagcc
     2041 agaatgtgtt atccgaacca gaggtattat ttagcttttg cagttgaagc tgcaaactac
     2101 taatttgctg ggtataacca ttttgggctg tttctaaagc tttaatccgt tctaaagctt
     2161 tttgatattc cgtgaccaat tcggcaattt gtgctttttc attttcgaaa tttggcatct
     2221 caaccatagt ttgagtagta ggctgatttg ctttagcaga tactgcctga atttgagatt
     2281 caacctctgc aaacttttta tttgccatat aatgaccagc tccgccaacc gctaaagcca
     2341 ctaatagtgc aactaatgcc aagccctttc cgcccgattt ttgaacaacc acttgttctt
     2401 ttggcgcttt ttgcatttct gtttgaatat ctttctctat agcttcgttc actacatctt
     2461 cctcaatctc tttttttgca aaagatcccg ctgaatcgac cgcttgttgt gcctcttcta
     2521 ccaccttttc agctgaatgc tctaaatgtt cagttacttt tttagatctt gacatactcg
     2581 ccacctctag ttagatttgc ccactttaaa taataatgta ttcatcaaag ttacattatc
     2641 ggctttatcc gataccctaa tcttatcttc ttgccagccc atttgtttag ctatctttgc
     2701 aatccgccgg ctcactacaa ccagatcaca ttcaaataac catgctctac aatctgcttt
     2761 cgcttgttca taaagtgatc ttaaaatttc actactggtg atcacaatag tatctactcc
     2821 caagcgttta cataagctga ttttttcggg aatatcatcg gtaaccggct ctctacgata
     2881 acactccaaa tattgaatag ttgccccacg caagactgca gtttcagcaa gtagctcacg
     2941 cccagaatcg gctcttagaa ttaaaatagt tttatcagtt agattctgca tttcaggtaa
     3001 tgccaaaacc ccctcgctat tttcaaattc tatcggataa atgaccgctt gttcagcttt
     3061 ttctgttaaa tatttcgcag tccgttgccc taccgcaaaa tatttcaaat ctgaacgaaa
     3121 atggaacccg gtatctacta atgtttttgc agcatagtcc actgcattcg gcgaaacaac
     3181 aaatacataa tcgtctgatt ttagccgaga aatagccgaa ggaagcaaag gtaattcttt
     3241 accggcttca atcgtaaata gcggttgatg aatagcaaaa atttgctgtt cattcaacat
     3301 atctaccaat tcttgccccc gactatccag gcgtgtgacc aacacattca ttattgtacg
     3361 ttttcgttat aaaccgcagc taaaatttta tctgcacctt gagcaagcaa ctgttccgcc
     3421 accaaaatgc ctaattgctc cgcctcttgc ttatgtcctt ttgccgaagc ccgaataatt
     3481 tgtgagccat ctaacgatcc aactaacgca ttaagcgtta attcatcgcc ctctaatgta
     3541 gcataaccac caatcggcac ttggcaaccg ccttgcaaac gggtattcat tgcacgttct
     3601 gccatcacgc aacaagcggt cgaactatgg tttaattttg caatgtagtt caaaatccgt
     3661 tcatcattgg ctcgagtctc aatccctact gctccttgac cagctgcggg taatgattgt
     3721 tccacagaaa tatagctacg gattcgctct tgcaagccta aacgaattaa gccggcagag
     3781 gctaaaataa tcgcatcata ctcgccatta tctaatttgc ttaagcgagt gccgacattg
     3841 cctcgcaatg atttaacctc taaatgcgga tatttcgcca ttaattggca ctgacggcgt
     3901 aagctcgatg taccgaccac agcacctttc ggcaaatctt ctaacgaatg gtagttgttc
     3961 gacacaaacg catcacgggg atcttcacgt tcacaaatca ccgcaagccc caagccttcg
     4021 ggaaagctca tcggcacatc tttcattgaa tgcaccgcaa tatcagctcg attttctaaa
     4081 agggctaatt ctaactcttt cacaaacaag ccttttccgc caattttggc taatggggtg
     4141 tctaaaataa tatcgccttt cgttaccatc gtaaccagtt cgacagataa ttgcggaaaa
     4201 tgtttttcta attcgctttt tacaaaattt gcctgccata aggctaacgg gctttgacgg
     4261 gttgcaatgc gtaaaatatc tttcataaat tctaaaatta aaacattgag ttaatgaatt
     4321 atcctaccac tttttagctg aactgccaaa aataatcgtg gatttcgctt tgcatttaat
     4381 caaaaatccg actttttctc
//
LOCUS       DANLUA010000191         4396 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLUA010000191 DANLUA010000000
VERSION     DANLUA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(71..1762)
                     /gene="dld"
                     /locus_tag="PXB44_002281"
     CDS             complement(71..1762)
                     /gene="dld"
                     /locus_tag="PXB44_002281"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL5282712.1"
                     /translation="MSNNSLISQLTEIVGQSYIITDPTKTEAYRSGYRFGTGSAIAVV
                     KPTTLLEYWNVLKACVAADVIVISQAANTGLTGGSTPSGNDYDRDIVIINTMRMNGIQ
                     LINNANQVVCLPGSTLNELELLLKTHGREPHSVIGSSCIGASVIGGICNNSGGALVQR
                     GPAYTEMALFAQLNEDGTLELKNHLGIDLGDTPEEILENLQAQRYQRKDIQQACGKGH
                     DHAYCNHVRQIDEDSPARFNADPARHYEAAGSAGKLAVFAVRLDTFPLEKDTAVFYIG
                     TNHTDTLNDIRRYMLANFEVLPISGEYIHRDAFDIAADYGKDTFWVIKKLGTHRLPQL
                     FSLKAKVDRMAKKLTFLPANLSDKILQAISKMLPQHLPESLRDYRNQYEHHLIIKMGG
                     KGVQEARDYLARYFADTQKGGYLECNPTESQAAMLHRFAVASAAIRYRAIHDKEVEDI
                     VALDIALRRNDKAWFEVLPPEIESQITHKLYYGHFMCHVFHQDYIVKKGVDCLALEHQ
                     MLELLDKRGAQYPAEHNVGHLYEAKPTLRKFYRQLDPTNSFNPGIGQTSKKKYWK"
     gene            complement(1974..3071)
                     /locus_tag="PXB44_002282"
     CDS             complement(1974..3071)
                     /locus_tag="PXB44_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5282713.1"
                     /translation="MFYSSNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALLNQNRRVPNIKNRVDESTEKAVVQFALDNPAFGQVRVSNELRKQGIFV
                     SAGGFCLCRWFLSLQVVFVSAGGVRSIWLRHHLANFKQRLTALEKLVAEQGIILSEPQ
                     IQALERKKEDEIACGEIETAHPGYLGSQDTFYVGNIKGVGRIYQQTFIDTYSKVAFAK
                     LYTMKTAITAADMLNDKVLPFFESQRLPMLRVLTDRGSEYCGKVENHDYELYLAINDI
                     EHTKTKVKQPQTNGICERFHKTILQEFYQVAFRKRIYMDLEALQADLDEWLMYYNHHR
                     THQGKMCCGRTPMETLVDGKRIWAEKNLSSN"
     gene            3401..4117
                     /locus_tag="PXB44_002283"
     CDS             3401..4117
                     /locus_tag="PXB44_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL5282714.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFYDIIRNNF
                     DYHNGRIHGLYLRNKEDIIS"
     gene            complement(4160..>4396)
                     /locus_tag="PXB44_002284"
     CDS             complement(4160..>4396)
                     /locus_tag="PXB44_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5282715.1"
                     /translation="RGQPMSQNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPEN
                     VYVVQKLLGHKDISVTLGYIEHDVEMLRETVNLL"
BASE COUNT         1273 a          921 c          832 g         1370 t
ORIGIN      
        1 agcaactgtt ttgtccactt ggcatagttt ttgacggatc agcaactatt ttgtctacta
       61 tgcctttttg ttacttccag tattttttct tcgaggtttg cccgatcccc ggattaaagc
      121 tgttggttgg atcaagctgc cggtagaatt tgcgtaaggt cggttttgct tcgtaaagat
      181 gaccgacatt atgctccgca gggtattgtg ccccacgttt atccagtaat tctaacatct
      241 gatgttccaa cgccaaacaa tctacacctt ttttcacaat atagtcttga tggaaaacat
      301 ggcacataaa atgcccataa taaagtttgt gcgtgatttg actctctatt tccggcggta
      361 gcacttcaaa ccacgcttta tcatttcggc gaagggcaat atctaaagca acaatatctt
      421 ccacctcttt atcgtgaatg gctcgatagc gaatcgctgc cgaagcaacg gcaaaacgat
      481 gtaacattgc cgcttggctt tcagtcggat tacactccaa ataaccgcct ttttgtgtat
      541 cagcaaaata acgggcaaga tagtctcttg cttcttgaac gccttttcca cccattttga
      601 taattaagtg atgttcatac tggttacggt agtctcgaag actttccggc aaatgttgcg
      661 gtaacatttt tgagattgcc tgtaatattt tatcactgag atttgccggt aaaaaggtga
      721 gtttcttcgc cattctgtcc acttttgctt taagtgaaaa gagttgtggt aagcggtgtg
      781 taccgagttt tttaatcacc caaaaagtat ctttaccgta atcagcggca atatcaaagg
      841 catcacgatg aatatattca ccggaaatcg gcaatacctc aaagttggct aacatatagc
      901 gacgaatatc gtttaatgtg tccgtatgat ttgtcccgat ataaaaaaca gccgtgtctt
      961 tttcaagcgg aaaggtatct aatcgcaccg caaacaccgc taactttcct gcactaccgg
     1021 cagcctcgta gtggcgggca ggatcggcat taaaacgggc aggggagtct tcatcaattt
     1081 ggcggacgtg attacaatag gcgtgatcgt gtccttttcc gcacgcttgc tgaatatcct
     1141 tacgctgata acgttgggct tgcagatttt ctaaaatttc ttccggcgta tcacctaaat
     1201 caatacctaa atggtttttt aattccagcg tgccgtcttc atttaactgg gcaaacagtg
     1261 ccatttcagt ataagccggc ccacgttgca ctaatgcacc ccctgaatta ttacaaatac
     1321 caccgataac cgaagccccg atacaggacg atccgatcac cgaatgcggt tcacgtccgt
     1381 gcgttttcag caacaattcc agctcattta atgtcgagcc cggtaagcaa acgacttgat
     1441 tggcattgtt aattaactga atgccgttca ttcgcattgt gttaatgatc acaatatccc
     1501 gatcataatc gttgccgctt ggtgttgaac caccggttaa tccggtattg gccgcttgtg
     1561 agataacaat gacatcggcg gcgacacagg cttttaatac gttccagtat tctaataacg
     1621 ttgtaggttt taccactgca atcgcactac ccgtgccaaa gcgatacccg ctacgatagg
     1681 cttccgtctt agtcgggtca gtgatgatat aagactgccc gacaatttca gtaagctgag
     1741 aaatcagtga attatttgac attatgcttc cctcaaaggt agaaatagat agggcagtat
     1801 agcaataaac aaaagagagc aaagcagaat gttacaaaat tgtgaactac tgcacagaat
     1861 aaatgaagaa atgaagtaaa agaaagcgag ctaaaagagc gtcgcccgct ttatttgtag
     1921 aaaatcaaac ctaatctgac agtcccccgt ttaaaattac cgtgtctgtc agattaattt
     1981 gagcttaaat tcttttctgc ccaaatgcgt tttccatcaa ctaatgtctc catcggtgtt
     2041 cttccacagc acatttttcc ttgatgggtt cgatgatggt tataatacat taaccattca
     2101 tctaagtcag cttgcaatgc ctctaaatcc atataaatcc gttttctgaa tgccacttgg
     2161 tagaactcct gcaaaatcgt tttgtgaaaa cgctcacaga tgccgtttgt ttgtggctgt
     2221 ttcactttgg tttttgtgtg ctctatgtca ttgatagcca aataaagctc ataatcgtga
     2281 ttctcaactt tcccacaata ctcactgcct cggtctgtca gcactcgtaa cataggtaac
     2341 ctctggcttt caaagaatgg taatactttg tcatttagca tatcagcagc agtgatagcc
     2401 gtcttcattg tatacagttt tgcaaatgcc actttactat aagtatcaat aaacgtttgc
     2461 tgataaatac gacccacacc tttaatattg ccgacataaa aggtatcttg tgaccctaaa
     2521 tagccggggt gtgccgtttc aatttcacca caggcaatct catcttcttt cttccgctcc
     2581 aaggcttgta tctgtggctc actcagaata atgccttgct ctgccaccaa tttttcgagg
     2641 gcagttaagc gttgcttaaa attagctaag tgatgtctca accaaatgga acgtacacca
     2701 cctgcagaga caaaaaccac ctgcagagac aaaaaccacc tgcagagaca aaaaccacct
     2761 gcagagacaa aaatcccctg tttacgcagt tcattactca ctcgaacttg accaaaagca
     2821 ggattatcta aagcgaattg tacaaccgct ttttctgtgg attcatccac acgattttta
     2881 atgttgggaa cacgtcgatt ttgatttagt aatgcctcaa caccgccttg ttcgacagcc
     2941 tgttgatagc gataaaaagt atctcgactc atccccatta ccttacaggc ttgtgagata
     3001 ttacccagtt cttctgctaa atttaataaa ccggtcttgt gtttaataag tggattgcta
     3061 gaataaaaca tgagagtttc ctttttgttt agattgattt ttgacactca tattctaaac
     3121 gggaaactct cactttttag agtgaattgt cagatcaagt ctgatcttct acagacgatc
     3181 caggtcttac acattctttc aagtttgtaa agcctgaaaa ttatttttta tacttgattt
     3241 ttgtcaaaaa gtctctacac tatatggcag ggtcagtttt cgctgtttaa cggtgtttaa
     3301 tgagattttc attactggct ctccttaata gaaatttatg gaagagctat ctgatagagt
     3361 aatctagcga tagctctttt tcattgaaca aagaggctat atgtatctta tgtatgttga
     3421 tgagtctggt tctacgacag atcctactca aaaatatttt gtattatcag gtatcgctgt
     3481 atttgaacga aatactcatt ggattgagaa aaaaatgaat gtgatagcct ctcaattctg
     3541 tcaatattct gaagggagtg atccatattt ctatgagtta catggttcac caatgagatc
     3601 tggaaagagt gaatggagag gcattccaat gagtgagcgt attaatgcca ttcaagattg
     3661 cttgaaatta gttcaaagta ataatattcg tatatttgca gcagttatag agcaagggta
     3721 ttctagtggt caggatacta ttacagaatg ttttgagcaa atatcatctc gatttgatat
     3781 gtatctgagc cgtttacatg cccaaggtaa tacccaaaga ggcatttcta tctttgataa
     3841 atcatcgact gagaagtcaa tccaagcact tgcaagaaca ttcaaaaata gtgggcatac
     3901 ttatgggaaa cttagaaatt tctcagaagt tccattattt cttgactcaa agtcatcgag
     3961 attaattcag ctagcggatc tagtttccta tgcgatattt agacactatg aacacaatga
     4021 tagctcattt tacgatatta taagaaataa ctttgattat cataacgggc gtattcatgg
     4081 tttatattta cggaacaaag aagatattat ttcataaaaa ttcgccccaa aagggagaag
     4141 tatctccctt ttgtattttt tataacaaat taaccgtttc tctcaacatt tccacatcgt
     4201 gttcaatata tcctagtgtc acactgatat ctttatgccc taacagtttc tgaaccacat
     4261 aaacattttc gggcttcttc attaattgag tagcaactgt atgtcgaaat ctgtgtggtg
     4321 atgaagtaaa accgaccaag tcagaaatat gccgaaatat atgtgtaagc tgattttggc
     4381 tcatcggctg tcctct
//
LOCUS       DANLUA010000192         4350 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLUA010000192 DANLUA010000000
VERSION     DANLUA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(76..693)
                     /locus_tag="PXB44_002285"
     CDS             complement(76..693)
                     /locus_tag="PXB44_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL5282716.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSADKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
     gene            complement(706..897)
                     /locus_tag="PXB44_002286"
     CDS             complement(706..897)
                     /locus_tag="PXB44_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282717.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAERLGVEAKTVRFMSSPAH
                     NKRVKYNGKAIRTVKVGLE"
     gene            complement(900..1220)
                     /locus_tag="PXB44_002287"
     CDS             complement(900..1220)
                     /locus_tag="PXB44_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282718.1"
                     /translation="MQKVYSEKAGEMMNPLNAFIHDSLVKLEIATLECEAMELEVERV
                     EFEAKSPPRLVLRDNCKTQDLVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            complement(1231..2112)
                     /locus_tag="PXB44_002288"
     CDS             complement(1231..2112)
                     /locus_tag="PXB44_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5282719.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTSVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACHGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            complement(2116..4053)
                     /locus_tag="PXB44_002289"
     CDS             complement(2116..4053)
                     /locus_tag="PXB44_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5282720.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYHINSLPTEVQTEIRSRFMSAVVEAKPKKLPVVRAEVDVGNLTTKQREIADARMAL
                     VAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLAELVAVANAKKTAKRTLSVRTLNGW
                     VVDYCKAENAEQRLKLLAPQVRQETKPEEIWWLSAFLGVYRQKNGICLTEAYREFEVE
                     WAYQYADNPLLLAQCPSLSQVQRAMNKLPLYVKEYGRRTGSHYKQLLSYVKRDWSVLR
                     ANDVWIGDGHSLKLKVAHPIHGKPFTPELTMIVDGAGRKVVGWSLALSENAFAVADAL
                     RHAISLHGVPAIYYSDNGGGEKNKFLDAEITGMLPRLGIRHETGIAGNPQGRGIIERL
                     NKTIGMTIARQFETGYATGADPETVRKTLYAVNSLANAKGELTPLQRKAQGKLPTWQQ
                     LIEVIQSVIDWYNNEHIHSEIRTTPARKYQQMLHAEDVVMLSPIELRDMSRPEFIRKP
                     DRGWVSWNNNHYFNLKLLDFDREEVVIGIDIHNAESVQVRTKDGRFICEAIWNGNTRE
                     AFPVAMVEQQRKERHKRRANLKQQQLDEINAELNPVLTIEQKNDFSLLATARKPKKEV
                     EPIFLTRAEKEDYEKKLAVGG"
     gene            complement(4068..4295)
                     /locus_tag="PXB44_002290"
     CDS             complement(4068..4295)
                     /locus_tag="PXB44_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282721.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
BASE COUNT         1007 a         1167 c          807 g         1369 t
ORIGIN      
        1 tctattgaac aaaccttgtc tgtattaacc acaacaggtt tatccgtata attaacatta
       61 aaagtaataa acattctaaa cccccgccat atctaaacta atcggctgat actttccgtt
      121 ttcatcacgt ttataaaaac ggatataacc tttactaccg atcacttgta cgctatctga
      181 aatcgctgtc attgcttgat tccatcttgc atcatcaatc tcaacacggc ggagagagag
      241 aatttttgct gtagataaat taccttcctt atccacctca aacgcattat caattaaggc
      301 cttaagctcc ggttttgccc cttccgacca atcgtgcaag cattcatcaa tcaaggcttt
      361 tgctgcgtga atacgctcat caaagcgaat attctcttgc actgctaact gcaacttata
      421 ttcaccgtca taagtaaata gtgtgatatt gccttttgca ccgccaattt ttactccata
      481 tttgtcagcc gataggctga caaaagcccc cacgtcatca aatacggatt gtttaaaatc
      541 acctaacgct ttgcttaacg cttgtgcttt gcttacaaat tggcgaacta attcatcacg
      601 ttctttgtcg atttctttta ctaatgcctc cggctttaaa ttgccatcag catctttcca
      661 gtaaatttca ttaccgattg ttactttact cataatactt cctctctatt ccaacccgac
      721 tttcacggtg cggattgctt taccgttata tttcactctt ttgttgtgag caggggacga
      781 cataaaccgc actgttttcg cctccacccc caaccgttct gctaactctt ccgccgtacc
      841 gtcagccaca ttctcttcgc caacatacag ggcataaatc attcgccgtt ttttcatttc
      901 tacaccttcg taacacgttc agactcccac acgcatttca ccccccgcac ctgcatttga
      961 tagctgtcat accgtctgcc gcctttggtt ttcattccaa acaggcttgc caagccgttc
     1021 ttgactaaat cctgtgtttt gcagttatcc cgtaagacca agcggggtgg gcttttcgct
     1081 tcaaactcca ctcgttcaac ttccaactcc atcgcctcac attccaaagt ggcaatttct
     1141 aacttcacaa ggctgtcgtg gataaacgca tttaaggggt tcatcatttc gcctgctttt
     1201 tcgctgtaaa ccttttgcat aacttgctcc ttagctaatc aacatcttgc tatattgctc
     1261 aaccaaatct ttactaattg gtgtttggtt aatctcgcta gaccgcacca cgccacgcat
     1321 taacttactt aatcggcggg cattgccgtg acaagcttta attagcgggg cattaaattc
     1381 atttgtacct aacgcacttt cggctaatag ccccaaatct tcctcgctca aggcatttcc
     1441 caaatcacaa gcaaaaccta ctcggctata aagctgtgcc agctcattat ttttaccttt
     1501 aagattaacc aacaatctcg gcataccggc taacaccacg cccacgctgg ttaaatcgtg
     1561 gattcggcgg ataaactcca gcgagcgggt cgaaagtaat tcagcttcat caatcattag
     1621 caacctttca gagcctttta acttgttcac aatgctttct aatagttcgt tatttacccc
     1681 tctggcattt gcccccaccg cctcggcaat cttgcggagc aagactttag gtgtgcaact
     1741 cggatcaacc tcaattaatg ttgcagagct gtttctgcgt tcgtattctt tgagcatttg
     1801 ggttttacct aagccggctg ccccaaaaat cacattaatt tcgccttcaa tatgggcaaa
     1861 ctgcattacc tccattcctc tgcgtgccgc caaagttggc acaaaagcag cgttatattt
     1921 cgcttccacc acttttgcct tatcacgctc aatcagctcg tctactttgc gatccatttc
     1981 agccacattg ccgttataat cgcctttgag atacagactg actgtggcaa cggatacacc
     2041 taaaagctgt gccacttgtt tttgttgata gcctttgcta tccattaagg ctctaagttc
     2101 ttggtttttc atcgtttagc ctcccactgc taactttttt tcgtaatcct ctttttctgc
     2161 tcgggtcaaa aaaatcggtt ctacttcttt tttcggtttt cttgccgttg ccagcaagct
     2221 aaaatcgttt ttctgctcaa tggttaaaac cgggtttaat tccgcattaa tctcatcaag
     2281 ctgttgctgt ttaaggttgg ctcggcgttt atggcgttct ttacgctgct gttctaccat
     2341 cgccaccggg aatgcctctc gggtgttacc gttccaaatt gcctcgcaaa taaacctgcc
     2401 gtctttggta cgcacctgca cgctctcggc attgtgaata tcaatcccga tcaccacttc
     2461 ttcacggtca aaatccagta atttcaagtt aaaatagtgg ttgttattcc aacttaccca
     2521 acctcgatcc ggcttacgga taaattccgg tcggctcata tcccgcaact caatagggct
     2581 taacatcacc acatcttctg cgtgcaacat ctgctgatat ttgcgtgccg gcgtggtacg
     2641 gatttccgag tgaatatgct cgttgttgta ccagtcaatc accgattgga ttacttcaat
     2701 caactgttgc cacgttggca atttgccttg ggctttacgt tgtagtgggg ttaattcccc
     2761 tttggcattt gccagtgagt tcaccgcata cagggttttg cgaacggttt ccggatctgc
     2821 accggttgca tagccggttt caaactgtcg ggcaatcgtc ataccgatgg ttttatttaa
     2881 gcgttcaata atgcctcgcc cctgcggatt gcccgcaatc cctgtttcgt gtcggatacc
     2941 gaggcggggc aacatccccg tgatttccgc atccaaaaat ttattctttt caccaccacc
     3001 gttatccgag tagtagattg ccggcacacc gtgtaggcta atggcgtggc gtagtgcatc
     3061 agccaccgca aaggcgttct cactgagtgc cagcgaccag cccaccactt ttctaccagc
     3121 accatcgaca atcattgtca gctcaggggt aaacggctta ccgtgtatcg ggtgggctac
     3181 tttgagcttc agtgagtgac cgtcaccaat ccatacatca tttgctcgaa gtaccgacca
     3241 atcccgcttc acatagctca gtaactgctt gtaatgcgag ccggtgcgtc tgccgtactc
     3301 tttcacatac aacggcaact tattcatcgc tcgctgtact tggcttaggc tggggcattg
     3361 tgccaacagt agcgggttgt ctgcatactg atacgcccat tccacctcaa attctcggta
     3421 ggcttcggtg aggcaaatac cgtttttctg ccgatatacg cctaaaaaag cacttagcca
     3481 ccaaatttct tccggcttgg tctcctgccg tacctgtggg gcaagcagtt tcaaccgttg
     3541 ctcggcgttc tctgccttgc agtaatccac cacccagccg tttaaggttc gcaccgagag
     3601 tgtgcgtttt gccgtctttt tagcatttgc tacggcaacc agctccgcca agtgcggtgg
     3661 catttcgcct tgtttggcaa ggttgcaaag gtaggttatc gctttaatcc ggctcattga
     3721 gccttccaac tccaacacat acgccaccag tgccattcgt gcatcagcaa tttctcgctg
     3781 tttggtggtc aggtttccca catccacctc tgcacgcact accggtaact ttttcggctt
     3841 ggcttccacc accgccgaca taaagcggga gcggatttcg gtttgaactt cggtgggtaa
     3901 agagttaatg tgatattcaa aaccacctcc tttaacccct ttcaatggac gagttgccca
     3961 ttgttctttt ttggcttgtc tagttacatt tgttgggtat tttgataagc cactaatacc
     4021 tgctaattct tttgcactaa accaatcttt cataacagct atccttgtta tgcaacatca
     4081 gggtaacgac taggccatat ttcggctggc gtttttccta atgcttttgc gataatttct
     4141 tcaccgtttt tataaggttt atcaagagca tttcttactg ttgttttgga aagcccattc
     4201 tcaatcccta actgagaaaa tgttattcct ttcttcatca attcagctcg aatatcagct
     4261 ctatgcatat cttgagctct tttcttttta ttcattttgt gagatcctcc acatttagtt
     4321 tatttgctac gctttagcgt acttgattaa
//
LOCUS       DANLUA010000193         4189 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLUA010000193 DANLUA010000000
VERSION     DANLUA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            142..2076
                     /gene="iolD"
                     /locus_tag="PXB44_002291"
     CDS             142..2076
                     /gene="iolD"
                     /locus_tag="PXB44_002291"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL5282722.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2203..3099
                     /gene="iolE"
                     /locus_tag="PXB44_002292"
     CDS             2203..3099
                     /gene="iolE"
                     /locus_tag="PXB44_002292"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL5282723.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3101..4111
                     /gene="iolG"
                     /locus_tag="PXB44_002293"
     CDS             3101..4111
                     /gene="iolG"
                     /locus_tag="PXB44_002293"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL5282724.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1224 a          916 c         1026 g         1023 t
ORIGIN      
        1 gcaataactt cgcagaaatg agaactggat cacatctttt tttcgatttc cttgctatta
       61 taaatgaaat aaatttttca tttgtaaaaa tcagtagaaa accgaccgct tgcaaaagcg
      121 atgtaagcca gaggaggctt tatgcagact actcgtttaa cggttgcaca ggcgttagtg
      181 caatttttgg ataatcaata tttagacgtg gacggcaaag agattaaatt cgttcacggt
      241 gttgccgcta ttttcggcca cggcaatgtg ctgggaatag gtcaggcgct tgctcaaaat
      301 gccggtggtt taacgctcta ccaaggcaga aatgagcaag gtatggcaca tttggcgatg
      361 ggctttgcta agcaaaatct gcgtcaaaga attattgcct gtacttcctc ggtcgggccg
      421 ggggcggcaa atatggttac ggctgcggca acggcaacag cgaaccgtat tcctcttctt
      481 ttattaccgg gcgatgtgtt tgcgacccgc cagccggatc cggtactgca acaaatcgaa
      541 caaccttacg atttaagcat tagcactaac gatgcgttcc gtgccgtcag taaatattgg
      601 gatagagtgc aacgccctga gcagttaatg agtgcttgta tcaatgcgtt tcgtgtgctg
      661 accgaccctg ctgaaaccgg tgctgtcacc attgctctgc cgcaagatgt acaagcggag
      721 agttttgatt tcccgaacag cttcctgcaa aaacgagtgc atcgcttaga gcgtacaccg
      781 ccgacaacag cgatgattaa gcaagcggta acattaattc aaaccaagaa aaaaccgctg
      841 attgtctgcg gtggcggtgt tcgctactcg caagcgggtc aaattctgca aaaatttgct
      901 aaacagttcg gcattccatt tgctgaaacc caagcgggta aaagtgcggt ggtgtccgat
      961 cacgcattga atgtaggcgg agtgggagaa accggttgtt tagcggcaaa ccgtctggca
     1021 aaagaagcgg atttagtgat tggcattggt acacgctaca ccgacttcac gacttcttcc
     1081 aaatggattt tccaaaaccc aaacgtgcag tttttgaaca ttaatgtggc acgttttgat
     1141 gcgtacaaat tagacggcgt gcaactggtt gccgatgcaa aaactgcatt agaggcgtta
     1201 agccaagcca ttcctactga ttatcaagct caatgggaga atgaaatttc acaggcgaaa
     1261 gcggattttg ctgatgaaat tcaacgcctg cgcgctgtgc aatatcaccc tgatttcacc
     1321 cctgaaatta acgataatct tgatcataaa gcggttcaca atgaatttat tcagctcacc
     1381 ggttcccagc tcacccaaac ccaagtgctg ggtattgtga atgaaaccat tgataacaat
     1441 gcgattattg ttgccgccgc cggaagtttg ccgggtgatt tacaacgcac ttggttaagt
     1501 aaaggtgaag agacttacca ccttgaatat ggctactcct gtatgggcta tgaagtgagt
     1561 gcggcagtcg gcgtgaaaat tgcccgcccc gaacaagagg tctatgcctt gctgggggac
     1621 ggctcttact taatggcaca caccgaatta gtgacctcaa ttcaagaggg caaaaaaatc
     1681 aacgtggtgc tgttcgacaa tatggcaaac ggctgtatca acaacctgca aatcggcaac
     1741 ggtatggaca gtttcggtac ggaattccgc ttccgcaatg gcgaaactaa ccaactcaac
     1801 ggcggtttag tgccgattga ttttgccaaa aacgccgaag catacggctg caaaacctat
     1861 cgggtaacta acgaagccga attgcgtgcc gcccttgatg atgcgaaaaa acagaccgtt
     1921 tctaccttaa tcgatattaa agtgttgccg aaaacaatgg ctcacggcta tggctgctgg
     1981 tggcacgttg gtgtcgcgga agtgtcagag agcgagcgta ttcaacaagc ccgccaacgc
     2041 tccgaagagc agttagctaa ggctcggaag tattagttaa aaaaacaact ccccactttg
     2101 agctacgagg ggagaatatc aagcggtcaa atttgcaaaa tttttgataa aaataaccgc
     2161 ttgcatccaa atttatctct ataaatcaaa cgaggtttct ctatgaaagc tgaaaacatt
     2221 caattaggca ttgccccaat cggttggacg aacgatgacc tgccggaact gggagcggaa
     2281 aacactttcg agcagtgtgt gagtgagatg gcacttgccg gctacacggg ctgtgaagtg
     2341 ggtaataaat atccgcgtga tgttgccgtg ttaaaacaca aactggacgt gcgtggcatt
     2401 caaatctgta atgcgtggtt cagcaccttc tttgtggacg gtaaacggga agagaccatt
     2461 gccgagttca tcaaacatcg tgatttcttg catgcaatgg gggcaaaagt gatcggctgt
     2521 tccgaacaga gccgtagtat tcaaggcaca accaaatctg tgttcaaaga aaaaccgatt
     2581 ttcaacgatg aagattggaa acgtatggca gaaggctata acgaattggc gaaacttgcc
     2641 gccgaaaaag gaatgaaagt ctgcctgcat caccatatgg gaaccggtat tcaaacaccc
     2701 gaagaggtcg atcgctatat ggcagaagtg aatgacgatg tctatctgtt gttcgattca
     2761 gggcatttgt actattccga aggctgccaa aaagcgatgc tcggcgtact ggaaaaatat
     2821 atcgaccgta ttatccacgt tcacttaaaa gatgtgcgtg atgaagtcat ggcggaagta
     2881 aaagcaaaag atttaagctt tttagaaggc gtgaaaaaag gcacctttac tgtaccgggc
     2941 gatggtgtca ttgatttccg tccgattttc gatattttag aaaaacacga ctacaaaggc
     3001 tggatggttg tagaagccga acaagatccg gcacttgcca acccgtttga atacgccctc
     3061 aaaggcagaa aatatatccg tgaaaccgca ggagtttaat atgttaaaag taggtattat
     3121 tggtgcaggc cgtattggac gtgtgcattc tgaaagtatt agcaaatatg tcaaaggtgc
     3181 tgaaatcaaa gcaatctctg atgtaaaaat taccgaagaa ttgaccgctt gggcaaaatc
     3241 gatgggtatt ccggaagttt atgatgacta taaaaaaatc ctgcaagatc cggaaattga
     3301 tgctgtattg gtctgctctt ccaccaacac tcacgctccg atttcgattg aggcagcaca
     3361 ggctggcaaa cacgttttct gtgaaaaacc ggtagatgca gacccagcta aaatccgtga
     3421 agtattgagt gcagtcgaaa aagccggtgt aaaattccaa gtgggcttta accgccgctt
     3481 cgaccacaac ttcaaggcga ttaaagaccg tgtggcggca ggcgacatcg gcgaaccgca
     3541 cgttatccga gtaacctctc gtgatccgga agccccgcca atcgaatatg tgaaagtctc
     3601 cggcggaatg ttctttgata tgaccattca cgacttcgat atgattcgtt atttatccgg
     3661 cagcgaagtg gaagaagtgt ttgcagtcgg caccgtatta gtaaaccctg aaatcggcaa
     3721 agcgggcgat attgatactg ccgttatcac gttgaaactg aaaaacggag caatcggcgt
     3781 aatcgacaac agtcgcaaag ctgcctacgg ctacgaccaa cgtgcggaag tgttcggctc
     3841 gaaaggggca atccatatca ctaatgatac cggctcaact gccgtattct caggtgaaaa
     3901 cggcgtggtg gcggaaaaac caaaatactt cttcttagaa cgttatatgc aatctttcgc
     3961 cgatgaaatc agctgtttcg tagattctgt ggtaaacgac aaaccaacgt tagtaaacgg
     4021 caatgacggc ttacaaccgg tacttattgc cttagctgcg aaaaaatcac tggaagaaaa
     4081 ccgaccagtg aaattgagtg aagtggcgta attgatcata aatacaagcg gtcggtttta
     4141 ctcgctacac tcgcacttcg tgccatcggc atagccgatg ttcaaacgc
//
LOCUS       DANLUA010000194         4067 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLUA010000194 DANLUA010000000
VERSION     DANLUA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(451..1545)
                     /locus_tag="PXB44_002294"
     CDS             complement(451..1545)
                     /locus_tag="PXB44_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5282725.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1679..2857
                     /gene="lpxB"
                     /locus_tag="PXB44_002295"
     CDS             1679..2857
                     /gene="lpxB"
                     /locus_tag="PXB44_002295"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL5282726.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2884..3936
                     /gene="sohB"
                     /locus_tag="PXB44_002296"
     CDS             2884..3936
                     /gene="sohB"
                     /locus_tag="PXB44_002296"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL5282727.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1215 a          698 c          887 g         1267 t
ORIGIN      
        1 aggaaaaatg tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg
       61 ggtggaaaag aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggggac
      121 tgtcagatta ggtttgatct tctacatata ataacccctc atcacttttt gcagtttaac
      181 ctcttttgca acagataatt taaatttagg cgttttggct gctcagttat tgccattatg
      241 attatattag ttcacactaa aattttagtg ttgtttctta aagctgaaga tgctaatagg
      301 tgagtaagcg gtttttacca ctttgaagat aaatgcaata gcttcttgtt tgaaggatta
      361 gttgtattta atcataaaaa atctgcacct taatcagttg gttagcccaa cttttaggtg
      421 cagatcattc tagtacgctt ttcagtttta ttaccaatat gctcgtaagc ctacaacagt
      481 cgtataattg cgagattttg cggtttttac atcattagta tagtgtttag cacgagttgc
      541 ttgccattca acaaagcctt ttaagtgttt tggaacaatg taatattccg ttcccaacat
      601 aaattggttt cttttaactt tgctatcatt tgccaagcct acagcattaa gtttgttttc
      661 tttataagca tacattgtgt aagcattcca ttgatcggtt aaacgatgga gtaacacagt
      721 gcgaacttct ttttcgatac gtttgtgacc gatagcattt tgatcttccg tttcacggcg
      781 ttctaaatct aagccaaggg tagtttttcc gtaggtataa gctgtaccga aggcaagagc
      841 aatgcgttca taattgccga tagattgctc agaacgctca cgggttgcac ctgttgctaa
      901 ttttaagctt tggttatcat caattttata gttataaatt gctgctgcac cccaaatatc
      961 tttacgttta ttgactaaat ctaagccatt gtttccacgt tggctatttc cgccatagaa
     1021 tgctccgagt tttaatcctt cgatgccttt gtaaacatat tgaagcgtac gtcgtgatga
     1081 accggtaagt aagccatcac ttaagctgag tgtatttggt aagtcagttt gttttacttc
     1141 atcggcaata ttagtcatat tgccgtatgt taattcaccg tattgtttat gcccgattcc
     1201 tgcatagagt tgatgatcat aaatatgatc gaagttatgt tgtgagcttg aatcgccacg
     1261 catacgccat tccacacgac ctaaagcata gaatccgtga cccacttgct gggttaaacg
     1321 gaaaccaaaa cgagagccgt tgttatccac tgcgtgatta acgtggttgc gggtagcttt
     1381 accatttacc ggtgaggttt tatctgaggt gctttcccac ttaatacgag cagaaccaat
     1441 aaattcaatt tgtgtaccgg tttctttatg atcgtaaatc gtaatagcta gagctgagtt
     1501 tgcgattaag gctgatgcta aaatcgctaa tgttgttttt ttcataaaaa tcactccata
     1561 tgaatagata aagggaggta gtgtatgtga atattcattg ctagacaata atgttttgtt
     1621 agtttatatg atgattggtt ataatagagt cacttacaga tcttcaataa tatttaatat
     1681 gaaacaacaa ttccccgtta ttggcttagt tgctggtgag gtctctggcg atattcttgg
     1741 tgccgggtta attaaggcgt taaaagtaca ttacccgaat gctcgattta tcggtgttgc
     1801 aggctctcga atgattgagg ctggctgcca gtctttgttt gatatggaag agctttctgt
     1861 aatggggcta gcagaggtgg ttaaacattt acctcgttta cttaagcgtc gtaaacaggt
     1921 tattgatgag atgcttaaat tgaaaccaga tgtatttatc ggtattgatg caccggattt
     1981 taatttaacc gttgaagaaa aactcaaagc gagtgggatt aaaacaatcc attatgttag
     2041 cccttcggtg tgggcttggc gacaaaagcg ggtgtttaaa attgccagag cagctaattt
     2101 agtgttggca tttctgccat ttgagaaagc gttctatgat aaattcaatg tgccttgtcg
     2161 ttttatcggg catacgatgg cagatgcgat cccactcgaa ccaaatcgta cagaggcatg
     2221 tttagcgtta aatattgatg aatccaagcg atatatggca attttggtgg gaagccgtgg
     2281 cagtgaaatt cagtttttgg ctgagccttt tttgaaggct gctcagttgt taaaagcccg
     2341 ttttccggat ttacagtttt tagtgccaat ggtcagccaa aagcgaatgg agcaatttaa
     2401 tgcaattaaa cagcaagtag ctcccgagtt ggaactgaat attattcaag gcaaggctcg
     2461 tcaggctatg attgcgtcag aatgtactct tttggcatcg ggaacagcag cactagaggc
     2521 aatgttgtgc aaatctccga tggtagttgg ttatagaatg aaaccgctga cttattggtt
     2581 agcaaaaaaa ttagtgaaaa ccgattatat ctccttaccg aatttattgg ctcaagcccc
     2641 attggtacca gaattgatcc aagaagcatg caacccggaa aatttagctc aagagttatc
     2701 tctattttta gctgatgatg agaaaaacca acaacataaa gcattgctaa aacagcagtt
     2761 tatgcagcta catcaatcaa ttcaatgtaa tgctgatgag caagctgccc aagcggttat
     2821 cgatctccta aacggctcga caagcggtca aatttagtta gttttttgta aaaaggaaaa
     2881 tttatgtgga aagaagtatt acttaattat ggtatttttt tattagagtt attaactgta
     2941 tttggcatta ttgcagtggt ggtgatgctg attttggaat cgaaaaagca gaatgaaaac
     3001 ggctcaattt caattaccaa tttatctgag aaatataaag agcagcaaaa atctctttcc
     3061 ggcttttttc taagtgagga agaattaaaa catcaggaaa agcaggagaa aaaagtagca
     3121 aaagagaaag ctaaagcaga aaaaaaacgc ttaaaagcag gcgaagaatg tgtagaaagc
     3181 ccttcccgtt tatttgtatt gaattttaat ggcgatatga tggctcacga agtaaattcc
     3241 ctacgccgtg aaattgatgc ggtaattagc ctcgcgaatc cggaaaaaga tgaagtttta
     3301 cttaaacttg aaagtccggg cggagtggtg cacggttatg gattggctgc ttcgcagtta
     3361 caacgtttgc gtgatcgcaa tatttattta accgcagcgg tagataaagt agctgccagc
     3421 ggtggttata tgatggcgtg tgtggcgaat aaaattgtct ctgccccatt tgcggtgatt
     3481 ggctcagtgg gggtggtggc tcaggtgccg aatatccacc gcttactgaa aaaacacgat
     3541 attgatgtgg atgtgatgac tgcaggtgaa tataaacgca cggtgacgct ggtgggagaa
     3601 aataccgaaa aagggaaaca gaaattccag caagaactgg aagaaacgca ctctctgttt
     3661 aagcaatttg tggctcgaca ccgcccacaa ctggatattg agcaggttgc aacaggcgag
     3721 cattggtttg gtcagcaagc gattgagcta aatttggttg atgagattgc aaccagtgat
     3781 gatctgctcc tcaatgcaat taaggaaaaa gaagtgatcg agctaaaata taaagagaag
     3841 aaaaaacttg ctcagaaatt gggttctcaa ttcgagcaat cagcagagaa cctgctcgct
     3901 aagattttac ataaaagtcg ttcaacgctg atgtagcgta tatattatta aagggctatc
     3961 gtttatatag ccccttattt tatcagttag tattagaatg aagtatttgt tcactattta
     4021 aatgacctgg aatacttttc tttaaattga gtgaagaatc taacaat
//
LOCUS       DANLUA010000195         4059 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLUA010000195 DANLUA010000000
VERSION     DANLUA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            181..1383
                     /gene="yedE"
                     /locus_tag="PXB44_002297"
                     /pseudo
     CDS             181..1383
                     /gene="yedE"
                     /locus_tag="PXB44_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            1419..1688
                     /gene="yedF"
                     /locus_tag="PXB44_002298"
     CDS             1419..1688
                     /gene="yedF"
                     /locus_tag="PXB44_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL5282728.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            1788..2132
                     /locus_tag="PXB44_002299"
     CDS             1788..2132
                     /locus_tag="PXB44_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL5282729.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(2195..3142)
                     /locus_tag="PXB44_002300"
     CDS             complement(2195..3142)
                     /locus_tag="PXB44_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL5282730.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            3325..>4059
                     /locus_tag="PXB44_002301"
     CDS             3325..>4059
                     /locus_tag="PXB44_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282731.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FHKLLVEIKSQYKSLYRYEALENHFEP"
BASE COUNT         1187 a          843 c          794 g         1235 t
ORIGIN      
        1 gcttgttatt tacatttgta caaaaaatac ctactataaa gacgcatttg taaaatgtgc
       61 attgatatat cgcaagtttt gtgtatagga aatgaactaa ttactgtgtc aatcagtccc
      121 tttcttcatc gttataccct cttgcaaatc cgattaatct atccttagtt aaggtgtttt
      181 atgttagcga tttggcaaag ttttaaacaa aattatctaa tcaaatattg gaatcctgtt
      241 gcagcagtga tcgcagctgg tattatttca gcttattatt tcggcattac tggcacttat
      301 tgggcggtta caggggaatt tacccgttgg ggcggtcatc tgttaaatgc tttgggtgtt
      361 gatgtttcga actggagcta ttttcaactc atcggcttag acggcaccat ctttacccgc
      421 attgacggtg tgatgattct cggtatgttt gccggctgtc tttctgccgc attatgggcg
      481 aacaacgtca aatggcataa tcagccacat aaaaaacgga ttatccaagc cttgattggc
      541 ggtgcgttag ccggtttcgg agcccgttta gcaatgggct gtaacctcgc ctctcttttt
      601 accggtattc cgcaattctc cgtccacgct tggtttttca cctttgcgac cgccatcggc
      661 acttactttg gggtaaaagt gactttatta ccgatgttta gagtgaaatt agagctgaaa
      721 aaaggggcag caaaaatcaa agaaaacgat ccgaaacaag ctaatcgccg tttttgggtt
      781 ggtatgctca tttttatcac ttatctttct gcttcccttt atgtaatgca atcctcaatc
      841 aaacttggtt ttgcaatgct ttgcggctta gcctttggct tattgattga acgagcccaa
      901 atttgcttta cctccgcttt ccgtgactta tggattaccg gacgggctta tatggcaaaa
      961 gcaattattt tcggcattat tgccggcaca atcggcgtgt tctcttatat tcaattaggt
     1021 gtttcaccta aaattttgtg ggctggccca aatgccatta tcggcggatt attattcgga
     1081 tttggcattg tgcttgccgg cggctgtgaa accggctgga tgtaccgttc aatggaagga
     1141 caggttcatt ttatgtgggt cggcgtaggc aatgtagtcg gttcaaccct gctggcttac
     1201 tactgaggcg acttagctcc ggtgcttacc cttgattatg aaaaaatcaa cctactcaaa
     1261 gaatttgggc cggttggcgg attattcatc aattatggct tattaattct ttgcttaatc
     1321 gctgtagttt ggtgggaaaa acgcttcctt gccaaagcga aagcaaaatt agcaactgcg
     1381 taatcttttt aacttcaacc aacaagagga aaatgactat ggcattcacc gttgtacaaa
     1441 aactagataa agatccaaaa gacatcacgc ctgattacac cttagatacc ttaggcgaac
     1501 cttgccctta ccctgcgatt acgatgttag aaacaatgcc acagctgcaa aaaggagaga
     1561 tattggaact gttaagcgat tgttcccaat cgattaataa tattccgatt gatactaaaa
     1621 atcacggcta taccctatta agcgtggaac aaaacggcac taaattacgc tatttaattc
     1681 agcgttaatt gcaaaaaatt cgcacaaaac gaccgcttgt cgtaaaatag caactattta
     1741 agagccttgt aacgcaaggc tcttttattc taagaggtaa aagaaaaatg aataaatacg
     1801 ctttacttgc aatattttgg tttattattg gcatttatgc tttacttttt cgtgaaagca
     1861 ctaatgatat accgcctttt ccacattttg ataaagtaag ccattttgcc ttattcttta
     1921 tccagatctg gttatctgca aacgccttta tccgttcaaa gaaagggata ccttattttg
     1981 ggctaataat ttttgcctta ctctatgctt tcaccagcga atgggctcag gcaaccttca
     2041 cctcaacccg agaaggttct tggcttgatg ggctagcaga tatgctagga gcctcacttt
     2101 cattattgct ggtaaaatat tgcaaaaaat aaagcataaa agaccgcttg ttatatggtt
     2161 tagccatact acaagcggtc attttttacc aaaattatgc catcaactca tcaatcaatt
     2221 ctttcacact tttgattttg ttgatttgtg aaatgccatt gcccaccgac acatagccat
     2281 tgtcaaaatc cccttctaac atacccaaac gcattcctgt accagcgttc attaccttag
     2341 caatctcact acgagttgcc ccattttcgc tcatttcaaa gcattttttc gctaatccag
     2401 tcggcagtga acggtagtaa tgtggttcgg tacggaataa cagcagatca ttggcatttg
     2461 cctccacaat ccagtttttc actttttcgc ttgcacggca ttcttcggtg gcgataaatg
     2521 ccgtgccaat ccacacgcct tcagccccaa gcgttaaggc agcctgcacc gcttttttat
     2581 cggcaatacc accagccagc attatcggca aatccacctt gcccacaaat tccgccagca
     2641 cattaaaact gcctacctct ttcatcggca aagtaccgcc ctcatcaaaa cccgtcagca
     2701 ccaaaatatc tgctcccaat gcttgggctt ttaaagcgtc cgccaaattg ggattaggca
     2761 aacggcagat gattttaatg ccagcagctt tgaatttggc gtaaatctct gggtcaagcc
     2821 agccgttatc cagcaccaca ggcacacgct cttcaatgaa taaatccacc attaacggaa
     2881 tcagcgaaaa atcataagac aaaataaatg gtacgccaaa aggcttatcg gtcaatgcct
     2941 taaccttacg aatctcgtta cgcatacggt caagtacttc ttcgttggaa gttggattgg
     3001 tggtttgccc tgcgtgtggg gctaaaaaac caagcccacc agcctcggca acgcttgcta
     3061 ccagatgagc gtccgtcagc caagacattg gcgcttggac gatagggtgc ttgatattta
     3121 agatttgggt aatgcggttc attggatttt cctgtttgaa taaaaagaat tggacgtagt
     3181 ttaatggttt tggatttgag agggaatgcg tgaaaatggg aaggtggttt tcggatttgg
     3241 aaaattatca atgctaaaat acttgaaata cacacgccaa tcctcatatc aacaaaattg
     3301 aatatttaat tatggtaaaa gaaaatgtct attagatggg aaaacatcaa gcccttacaa
     3361 ggttctcaaa atagtgcttt tgaagagcta gtatgccaat tggcacgcca agaatttaaa
     3421 tctcaaggaa aatttactcg aatttctgca cctgatggtg ggattgaagc aatgtgtgaa
     3481 ttgccagatg gttccgtatg tggttggcaa gccaaatatt ttttaagctc attttcttca
     3541 tcacaatggc aacaaattga agattccttt aaagaatctt taaaaaacta tcctaaacta
     3601 accaaatact atgtttgtgt ggcaaccgat agagcaaatg ccaatgtatc tggaaagaaa
     3661 tcatttctta ccaaatggga ggagtatacc caaagatgga aagactttgc tcaatcacaa
     3721 ggtcgtgaaa tagaatttga attttgggga agttttgaat tatctgactt attatcaaaa
     3781 cctgaaaatg caggcaaaaa attcttttgg tttaacgaaa gcgaattatc tgacaaatgg
     3841 tttgaacaat acaatcaaca agccattaat aatcttggtg tgcgttatac acctgaaatt
     3901 aatgttgatt tacctattgc aatgcaatta gaaagcttgg caagaaccca aattttcaaa
     3961 aaacatctga gtgatgaatt tcataaattg ttagtagaaa ttaaatcaca atacaaatct
     4021 ctttatcgtt acgaagcgtt ggaaaatcat tttgaacct
//
LOCUS       DANLUA010000196         4003 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLUA010000196 DANLUA010000000
VERSION     DANLUA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(106..1746)
                     /gene="pgi"
                     /locus_tag="PXB44_002302"
     CDS             complement(106..1746)
                     /gene="pgi"
                     /locus_tag="PXB44_002302"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL5282732.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1779..2027)
                     /locus_tag="PXB44_002303"
     CDS             complement(1779..2027)
                     /locus_tag="PXB44_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL5282733.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2151..2195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2199..2867
                     /gene="queC"
                     /locus_tag="PXB44_002304"
     CDS             2199..2867
                     /gene="queC"
                     /locus_tag="PXB44_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL5282734.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2860..3282
                     /gene="queD"
                     /locus_tag="PXB44_002305"
     CDS             2860..3282
                     /gene="queD"
                     /locus_tag="PXB44_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL5282735.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3282..3908
                     /locus_tag="PXB44_002306"
     CDS             3282..3908
                     /locus_tag="PXB44_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL5282736.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1197 a          748 c          851 g         1207 t
ORIGIN      
        1 aaactgcttg tgtagaaatg gcgcttagtt cagtcctcag ctaaaatttt agaaaaaaag
       61 catagagatt ttttacctct atgcttttta ttgtattgac tatatttaac gccaagcctt
      121 gaattggtta attaaaccat ttgtggagct gtcgtggctg ttgatttttt catcggtttc
      181 gagttcgggt aaaatgcggt ttgcaagctg tttgcctaac tccacgcccc attggtcgaa
      241 actgtaaatg ttgaacagca cgccttgcac gaagattttg tgttcataca ttgcaattaa
      301 tgcccctaag ctgtacgggg tgattttttg caccaagata gagttggtcg gtttgtttcc
      361 gctgaacact ttaaacggca cgatttcagc tacttcttcc aacgttttac ctgcatctaa
      421 aaactctttt tcaactgttt cttttgattt accgaaagct agtgcttcag tttgagcaaa
      481 gaagttagaa agcagtttgt tgtgatgatc gcttaacgga ttgtgagttt gtgccggtgc
      541 gataaaatca caaggaatta attttgtgcc ttgatgaatg agttgataga atgcgtgctg
      601 accgttagtt cccggttcgc cccaaataat cggacctgtt tggtgagtaa ccggtttgcc
      661 gtcacgcccc acaaatttac cgtttgattc catattgcct tgttggaaat aagccgcaaa
      721 gcggtgcata tattggtcgt aaggtaaaat cgcttctgat tcagcaccta agaaattgtt
      781 attccaaatg ccgactaaag ctaacgtagt tggaatgttt ttctcaatcg gcgtgattaa
      841 gaaatgctta tccattgcgt gagcaccagc aagtagctgc tcgaagttct caaaacctaa
      901 tgaaagggca atcgaaagcc cgatggcaga ccataatgag taacgaccgc caacccaatc
      961 ccagaattca aacatattag cggtatcaat accgaatttt tccacttctt tggcattggt
     1021 tgaaagtgcc acgaaatgtt ttgctaccgc agattcatct ttggcagaat caagtagcca
     1081 tttgcgagct gaaagggcat tggtcatcgt ttcttgagtg gtaaaggttt ttgaagccac
     1141 taacactaac gtggtttcag gattaacttt acgcaacact tcagcaatat gcgtgccgtc
     1201 cacgttagac acaaagtgca tattaaggtg atttttataa gggcgtaatg cctctgttac
     1261 catataagga cctagatccg agccaccaat accgatattg atcacatcag taatcgtttt
     1321 gccggtatag cctttccatt cgccggaaat aacacgctcg cagaaatctt tcattttcgc
     1381 taacacggca ttcacttccg gcattacatc tttgccatcg actgcgattg gtgtattggc
     1441 acggttacgc agtgcaacgt gcaatacggc acggttttca gtgcggttga tttttttgcc
     1501 ggagaacatc gcattttttg ctgattccaa accacattca tttgccagtt ggcgaagtaa
     1561 ggttaaggtt ttttcgttga tcgcattttt tgaaaaatcg actaataatt gatcttcaaa
     1621 acggagggaa tatttgtcga aacggtttgg atcttcggca aaaagttgag gaatagaaag
     1681 gttgtctgct ttatgttgct ctaaggcatt ccaagcaaaa gtttgcacag ggttaatttt
     1741 ttgcatctga gtttccttat actaaattaa aaaatcgatt attcatctgt tctttgagtc
     1801 agttcatcaa gacgtttttc taattcggtt aatttttcac gagtgcggag taacacttga
     1861 gattgaacat caaattcttc acgagtgaca acatcaagac gggctaattg ggcttgaagg
     1921 atttgcttaa atttttcatc tagatcatta ccgacatttt ttaagctttg cggtaaggcg
     1981 ttatggagtt gttgagcaat cgcttctaag ttcttcggcg tgagcatatt taaattcctt
     2041 actataaatg agtatcccaa ttttattgaa ttttgggttt aagagaaagc aaattctttg
     2101 taaaatatca cctttcgcaa aatacaagca aaaaagaccg cttgtatttt ttatttgtgg
     2161 ttcgtaaacc tcccacacaa aaaaactaag gacacaaaat gactcatcaa aaaccaaaag
     2221 ccgttgtgat tttttccggc ggtcaagact ctaccacttg tttattccaa gctattgctg
     2281 aatttggcaa agaaaatgtg gaagtcgtta cctttcaata tgggcaacgc cattcgattg
     2341 aacttgaaaa agcacgctgg atttctaacg atttaggcgt caaacaaacg ctgattgata
     2401 cttcggtgat taaagcgatt actaccaatg cgttaatgga taataatgct gaaattcaac
     2461 aacaaggcag cacacccaat acctttgtgg atgggcgtaa tgccttattt ctgctttata
     2521 ccgccattta tgccaaaggg cagggcattc aaactatttt taccggcgtg tgtgagaccg
     2581 attttagtgg ttaccctgat tgccgagatg tatttgtaaa atcaatgaat gtaacgctaa
     2641 atttagcgat ggactataat ttcaacattc gcacgccatt gatgtattta actaaaaaag
     2701 aaacttgggc attagcagat aagctgggtg cttttgatta tattcgagac catacccaca
     2761 cctgttattt aggcgttgaa ggtggttgcc acgactgccc gagttgcgta ttgcgagaaa
     2821 aaggcttgaa tgaatattta gcagaaaggg aggtaagcaa tgtttaaaat tgctaaagaa
     2881 tttagttttg atatggctca tatgctagat ggacacgatg gcaaatgcca aaacttacac
     2941 ggacacacct acaaattgca agttgaggtg gcaggtgagc tatttcaaga gggagctaaa
     3001 agaggaatgg taatggacta ttcggatcta aaagcagtgg taaaacaaca tattttagag
     3061 ccgatggatc acgcttttat ttacgactta cagagtgagc gtgaaagcca agtagcacaa
     3121 ttattaattg atttaaactc caaggtttat ggtatcccaa ctcgtaccac agccgaagaa
     3181 atggcgaaat atatgtttga aaaattagag aaagtgggct taccggttag cctaattcgc
     3241 ttgtgggaaa cgcctacttc ttattgtgag tacagcaaat aatggcggag ttaatttcaa
     3301 accctcgttt tccgattgtt gagatctttg aaagcttaca aggggaaggc tttaataccg
     3361 gaatgccggc tatttttgta cgttttggca aatgcaacct cacttgcccg tggtgcgata
     3421 ccaattacaa tcaatttgag caatggacgt taagcgatat tcttgccaaa gtaagaagct
     3481 attctgcgaa aaatgtgatt attaccggag gcgagccaac gattcagcct aatttatcgt
     3541 tattattaga acaatttaaa aaagagggat attttttagc gatcgaaacc aacggcttaa
     3601 aagaggttcc ggcacaaatt gattatattg cgaccagccc aaagcgaatg taccaagaaa
     3661 agtatcaacg ccgttgcatt gaatttgcga atgaagtccg tatcgtcgtt gatggagaag
     3721 tacagggttt ctgtgagcaa atagaaagcc aaattaaagc tgagcattac tatctttcac
     3781 cttgtgaggt agagggaaaa atgaacttgc tggaaaccat cacccagatt gggttattaa
     3841 atcagcgtcc aaataagcct aaatggcagc taagtatcca aacgcataaa attgtgggga
     3901 ttgaataagt agaagttaga agatgatttg taaaagatca gacttgatct gacaattcac
     3961 tctaaaaagt gagagtttcc cgtttagaat atgagtgtcc aaa
//
LOCUS       DANLUA010000197         4000 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLUA010000197 DANLUA010000000
VERSION     DANLUA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(70..957)
                     /gene="galU"
                     /locus_tag="PXB44_002307"
     CDS             complement(70..957)
                     /gene="galU"
                     /locus_tag="PXB44_002307"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL5282737.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(1093..1278)
                     /gene="csrA"
                     /locus_tag="PXB44_002308"
     CDS             complement(1093..1278)
                     /gene="csrA"
                     /locus_tag="PXB44_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL5282738.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(1368..3995)
                     /gene="alaS"
                     /locus_tag="PXB44_002309"
     CDS             complement(1368..3995)
                     /gene="alaS"
                     /locus_tag="PXB44_002309"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL5282739.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT         1134 a          914 c          682 g         1270 t
ORIGIN      
        1 aaatagaaaa gactggatcc aatagaatat cagacacagc cccttagatc atagtctaaa
       61 ctctaaggct tagagtgatt tagctaactc tttaataaat gctttaaatt cttttccgca
      121 tttgtcatga tttaaactat attctacaaa tgctttcata tagccgactt tataaccaca
      181 atcaaatgag cgccctgtca tatgaaaagc ttcaaccgtt tcttggtgaa tcaacatatc
      241 aattgcatcc gtcagctgga tttcatcacc tacgcctact ggcgtttttt ctaataaatt
      301 ccaaatattt gaggaaaata cataacgtcc tactaccgct aaattagaag gcgcctcttc
      361 aatacttggt ttttcaacca ttttcgcaat aggcgctgcc cccccgagag ggatatctac
      421 accatggcag tcagcaaccc catagctact tacattttcc attggaaccg gcgcaaccat
      481 aatttggcta tatccggttt cttggaaacg cttcaccatc gcagctaaat tttccgtttt
      541 ttggtttgct gtaaattcac caagtaatac atctggtaat acaaccgcaa acggttcatc
      601 gccaaccact gttcgtccac atagaaccgc atgacctaaa cctttggctt gcccttggcg
      661 aacgtgaatt aaggtaacat cttttggtac aatagaacgt acatcatcta ataattgacg
      721 ttttactcgt ttctccagca tcgtttccaa ttcaaaagag gtatcaaaat ggttttcgat
      781 agcattcttt gaagagtgag taaccaatac aatttcttta aaacctgcag ccacacattc
      841 atttacaatg tattgaataa gaggtttatc tgcaaggata agcatttctt ttggaatagc
      901 tttggttgcg ggtaacatgc gcgttcccaa tccagcaaca ggaataatga ctttcatttg
      961 gaatcctttg atgacttaac atattagata taactgaaat aaaccgctta gacataacag
     1021 gcggtcaatt ttcagaaaat tttgcattgc ctgcttcgct gttatcatct agcagcttta
     1081 cagttaaaat gcttattccg cattttcctc ttctactgag gcttttattc tttcataaat
     1141 ctctcggcgg tgtacagaga tttcttttgg tgcattcaca cctaatttaa cttgatttcc
     1201 tcggatgctg agtaccgtaa tctcaacatc atctcctacg agtaaaactt ccccaatctt
     1261 acgagttaga ataagcataa ttgtctccta aaaagtgaaa aactctataa atacttaaat
     1321 atagagtaaa gttttaaagg cgagttcagc tcgccttgtt attattttta aagttttgct
     1381 gtaatccatt cttgagcaag tgacaatgct tgcaataaat gttgtggttc tgagcctccg
     1441 gccattgcca tatcaggacg tccgccccct ttacctccca ctttttcagc cattgcacca
     1501 actaattgac cagcattgac tttatcggtt aaatctttgg tcacacctac aatcaaattg
     1561 actttatctt ccgccgtagt cgcaaataca acaactgcac tgcctaattg attttttagg
     1621 tcatcaacca tcgttctcaa tgcttttggt tcaacattct ctaattgttg aatcactaca
     1681 ttcacgccat tgatttgggt agcttgtttg gctaaatctg aacctgcttg tgctgcaagt
     1741 ttatctttta gctgttgaag ctctttttct gtacgtttag ccttctcttg taattgaaca
     1801 attttctcaa cgaatgaatg actgtctgct tttaacaatt cagcactttg ttgaacggct
     1861 tgttgtaagg tatgcaacca agcaatcgca ttttcaccag tgacagcctc tatacggcgt
     1921 acacctgctg ctaccgcacc ttcagaggta attttgaata agccaatatc accggtttgt
     1981 ttaacgtgtg tgccgccaca aagttcaatc gagaaatctg acatttccac tacacgcact
     2041 acttcgccat atttttcacc aaataacgcc attgcacctt tcgcttttgc agattcaata
     2101 tccatttctt caatgattac ctgaatattt tcacgcactt tagcattcac gatacgttca
     2161 acttcttcaa gttgtgattt agtaattgct tccggctgag aaatatcaaa acgtaaaata
     2221 ttttctgaca ctaatgagcc tttttgagca acgtggtcgc ctagcacttg gcgtaacgca
     2281 gaatgcagta agtgggtagc actgtggttt aatgtaatag catgacgacg ctctctatct
     2341 acgatcgtat taactttgtc gccaacagaa agtgagccgg aagttaattg cccaatatgc
     2401 ccgaatactt gaccatattt ttgcgtatct actacctcaa aattgcaaat ttctgcggaa
     2461 atttgaccgc tatctcccac ttgtccgccc atttctgcat agaatggggt tttatctaaa
     2521 atcactaccg cattttcacc cgattgaata gactcaaccg attttccatt actaaataac
     2581 ccaattacag tagcatcatt taagctggtt tcagtatatc cactaaagca tgttttactg
     2641 tccactttaa tcacattgtt ataatctgtg ccaaagctac tgctggcttt cgcacgctca
     2701 cgttgtgcgg tcatttctgc attgaagccg tcttcgtcaa ttttaatatc acgttcacgg
     2761 caaacatctg cggttaaatc taacgggaag ccataagtat cataaagttt gaacgcaact
     2821 tcgccagata aggtgttatt ttcaaccttc gctaatgctt cttctaataa attcaatcca
     2881 cgctcaagag tacgggcaaa ttgctcttct tctgctttga gtgtcttttg aatatgcgct
     2941 tgtttttggg tcagcacctc accagcttgc cccattaccg ttgcaagtgt tggcaccaat
     3001 ttatagaaaa aggcttcttt tgcacccaaa atattaccgt gacgaaccgc ccggcggata
     3061 atacggcgta atacatagcc tcttccttca tttgaaggta aaacaccatc ggcaatcaga
     3121 taggaacaag cacgaatatg gtctgccact acacgtaaag atttattatc taaatctttc
     3181 acattaagca aagtagcgac ttctttaatt aaagtttgga aaatatccgt ttcgtagttg
     3241 gagtttacgt gttgcattac cgcagtcata cgttctaacc ccatccctgt atctacagaa
     3301 ggcttaggca gtttttccat cgtaccgtct gctaaacgat tgaactgcat aaatacaata
     3361 ttccatacct caatatagcg gtcgccatct tcttctgggc tacccggtaa gccaccccaa
     3421 tgttcaggcc cgtgatcata gaaaatctcg gtacaagggc cacaagggcc ggtatcgccc
     3481 atcgcccaga agttatctga agcataaggt gcgcctttat tatcgccaat gcgaataata
     3541 tgttccgatg gtacacctac ttctttatgc caaatatcgt aggcttcatc atcagtttca
     3601 tacacagtta catataattt ttcttttggt aagccaagcc attgtggaga agttaaaaac
     3661 tcccaaccga atttgattgc atcttgcttg aagtaatcac caaagctgaa attccccatc
     3721 atttcaaaaa aagtatgatg gcgagcggta taacctacgt tttctaaatc attgtgttta
     3781 ccacctgcac gtacacaacg ttgtgccgta gtcgctctgc tataagggcg tttttctaac
     3841 ccaagaaaaa catctttgaa ttggttcatg cccgcattgg taaataataa tgtcggatca
     3901 ttctcgggaa caagagagct gcttggaaca atcgtatgtc ctttagtttg gaaaaactct
     3961 agaaaagatt gtctgatatc tgaagttgtt ttcattaaaa
//
LOCUS       DANLUA010000198         3993 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLUA010000198 DANLUA010000000
VERSION     DANLUA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..270
                     /locus_tag="PXB44_002310"
     CDS             <1..270
                     /locus_tag="PXB44_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282740.1"
                     /translation="NGGDEIEKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQE
                     VKNAINSGELGRKIEKFRRENPNIENDKNFKFPTSVTLSNWYSKA"
     gene            348..599
                     /locus_tag="PXB44_002311"
     CDS             348..599
                     /locus_tag="PXB44_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282741.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            599..979
                     /locus_tag="PXB44_002312"
     CDS             599..979
                     /locus_tag="PXB44_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5282742.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1290..2351
                     /locus_tag="PXB44_002313"
     CDS             1290..2351
                     /locus_tag="PXB44_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL5282743.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2344..2508
                     /locus_tag="PXB44_002314"
     CDS             2344..2508
                     /locus_tag="PXB44_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282744.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2501..3136
                     /locus_tag="PXB44_002315"
     CDS             2501..3136
                     /locus_tag="PXB44_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282745.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3130..3348
                     /locus_tag="PXB44_002316"
     CDS             3130..3348
                     /locus_tag="PXB44_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282746.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3490..3975
                     /locus_tag="PXB44_002317"
     CDS             3490..3975
                     /locus_tag="PXB44_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282747.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1446 a          840 c          771 g          936 t
ORIGIN      
        1 aacggcggag atgaaataga aaaaatatca aagaataaca aaactcaatt cagctcaaca
       61 atacgagaaa accaaatcaa ttttattaga gccttgcttt atatggatta tgggatagaa
      121 acgcctcaag aagtaaaaaa cgccattaat tcaggggaac taggcagaaa aatagagaaa
      181 tttaggagag aaaaccccaa tatagagaac gataaaaatt tcaaattccc tacaagtgta
      241 acccttagca actggtattc aaaagcctaa aatattggaa atttccgaat ctactttgga
      301 aatttccaaa cttctaaaac ctactcacct cacaatctcc cccaacaatg caactacaaa
      361 agacaataag cacattcacg caaccaaacc acagggggat ttatgtaatg acctattcag
      421 aacaaagcta tttaaacgtg aaagatgtat gcgaaatgct acaactcaac cgctcaacta
      481 tttatcgaca aattaagcaa gggattttcc ctaagccaac taagatcgga aaatcaagcc
      541 ggtggaaatt tgccgacatc aaagcatttt tagagccaaa accacaggag gcgaactaat
      601 ggcagggctt tctattattt gcccgttttg tggcaatacc gcacgaatta gaggcagttc
      661 tcgccctagc cttttaagtt atcaagcaca gctaacttgt tcaaattgcg gacaattaaa
      721 agccgacttt gtagggcagc taatcaacat caaacgggcc gtatttattg agtgtgatga
      781 agcaaatcaa tgggataaac ccgaaaatga actaatcaag gagggaaaaa ttcaaggcaa
      841 agacaatgct caacgcctca aagaactaag aggctcacag caagatttat ttgcaaaggt
      901 gccggggcaa ctaccgcccg agaagatcac tccggcggag aaaatcgcca agcgggcaaa
      961 cctttcaaaa acgcattaaa cagggggata aatggcaaca aaaaagcccc gcaaggcaaa
     1021 ccatacggag ctattaaaat cgcataatgc gaaagatatg caaagaaatt aaagcaggct
     1081 tgacgatttc tgaaggtgtt agattctttc taaagtcttt agggataggt ttatttctag
     1141 cattgttttt ttattttttg ccgtatttag cagaatttat aatcgccgta aaaaaacaac
     1201 tagcaaaaaa atcggcttac tttcaatgat aaaagcaagc cgaaatgaca atccaaattt
     1261 ttaactttaa ataaagagta caagaagaaa tgaaagattt atctccaaat cagaaacaat
     1321 atacgccatt ttcggctata atgcaaaaaa atttaatgga tttattgaca aaatccgccc
     1381 gttcgcctta ttctttctct accgttgcaa aatcaacggc agggcgtgag aaccctgaac
     1441 tacataatac ggcgaacagt agcacgcctt taacccgtgc tatttttatt cgtagcttac
     1501 acacacctaa agaacgccga aataaggcgt gttcgtctat ggtagcgtgt agcgggcaag
     1561 gttcgccctt ggctgttttt ggttcgccgt ttgtagcagt tttctcaccc cgttacacgc
     1621 tatcgccctg caccgtgaga agtgtagccg atagtccttt aaaattaaca aacggactac
     1681 aaaaaatgaa ccctagccaa attacaggca aaaaccgcct aaactctttt ctatttaaca
     1741 ataatcaaaa tgattattcg ctattattta ctaatgcaaa acaatcaacc ccgatttctg
     1801 ctaaaataag caaaaatcaa gatttatgct tgacaaccct caatcgttcg ccttatgcta
     1861 gccgtgcttt tgcaaaatca aaagccgagt gtggaaactc gaaagaacat caggcgaaca
     1921 atagcacgcc tttaactcgt gctttttttg ttcgtagctc acgcacaccc aaagaatgcc
     1981 aaaataaggc gtgttcgtct atggtagcgt gtagcgggca aggtttcgcc cttgactgct
     2041 tacctgatgt tgcagttttc caccccgtta cacgctatcg ccctacattg tggaaaatgt
     2101 tagcgatagc ccctaaaaat caacatcagg agctatcaaa aatgaaagca ttcgcttatt
     2161 cattcctttg tgctattcgc acacccgaaa cagatactta tcaagaaacc gtagtacgca
     2221 ttatttcaga cagcgaacaa aacgcccgtt ttcagcttac agccgattat cgcttactac
     2281 tcccccgccc tgttgcaaaa atccgcctaa attctaccgc ttacacggtg aaagggggga
     2341 tttatgctta attattccga tattgaacaa atccgctatt ttatggatca agccgaagag
     2401 gctttagaca ataatgaaga gttcaaagcc gtagcagaac ttaacacagc acaacaattt
     2461 attctggctt tattaatgaa ctacgaacaa gggggtgaaa atgaataata caacaggaca
     2521 agctacgcaa agaaactggc aagaaattat cgctaatttg cagagactca gcgaatccgg
     2581 ggaaagcaca atcgaaatcg ctaaacgcta tgaggcattt tctaaaaccg ccgagcaaat
     2641 ccaagcccta gtgaataaca acggcagcga agccgaaaaa gaggcgtttt ttgcccctgt
     2701 tgagcgtttt tatcttggct tactagaaac cgaaacacgc ttaaaagatg ccatagccaa
     2761 agacgaagaa aacgcccaaa aattgaaaga gcaaaaagca gctagagagg cagagcaaaa
     2821 aagatgggac aacctacctt tctacgccaa agacaaattc agtgatttaa tttgcattga
     2881 aagccgagaa acgcttgaag ataaattctt tgcgatctct tgtatgttcg atgccctaaa
     2941 agccgtacct aatctattag aaataggctt gcgtgaagaa ggcaaagaca gtgttataga
     3001 ccgattagcc gaaatgagca aactctattt actgtttgta gatgagcaat tttcactact
     3061 ggcaaacgaa attcaaagcg gtaaatatgg caagttagac ctaaacggct cacagaataa
     3121 gggggattta tgctaacgca ccgcattaca ctccctgacg gcacagaaat tgaatttttt
     3181 gcagaaaccg acaaagaaac atttgcagaa attcaaaggg acttagaggg cttgaaattg
     3241 ccaacctttg ccgagtttgt tgcccaagag cggaaaaaac accgagcaaa acaagcggtc
     3301 aaaaaagccc gaaaaattgc aaaattagca agacggaaaa accgctagca cctatcaaaa
     3361 agacttatta ttgcagaaaa tcacgcttta ataaaaacga cctataaagc aatccgcaag
     3421 aaatggttaa gtttaactga aaatatgagc ggacaggttc agcgggtagc gtgcgataga
     3481 ttcgcaataa tggaaaccgc cttgcagtta gcccaacatt taacaggctg gacagaggaa
     3541 gagaacagcc aagcgatttt aaaaaacttc ctagactgga aagaaacttt cggcgaaaaa
     3601 acaagagaag aaactaaaat agtagaaacc ttgctagatt ggataaatga aaatgaggcg
     3661 agttttatcg agtacccatt caaccaagag caaagaacac cgcctaaagt tgccggctat
     3721 agaatacttg cgaataacag cactaaaaca aatgagtact ttttcattta ccctaagcaa
     3781 tttaaagaaa cgctggcaga atttccgcta ttgatggctt gcaccaagtt acacgaggca
     3841 gggattttac ataagccacc acgcccagaa aaaggctatg aatttcagta cacaatagcc
     3901 cctaaaataa caggagggag caaaaaaaga gcttataaaa tcgtaccgat agcattggaa
     3961 gaagaaaacg agtaattaca gcaaaagcaa gag
//
LOCUS       DANLUA010000199         3753 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLUA010000199 DANLUA010000000
VERSION     DANLUA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2437
                     /locus_tag="PXB44_002318"
     CDS             <1..2437
                     /locus_tag="PXB44_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL5282748.1"
                     /translation="GGVVITADLEFENLLRDTQKVDKAMDDIGKSTLKAEKGLKGLET
                     QATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQMGSNASTVIAQQGSQILSIF
                     GPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAAQKNLSEVVTQTESGVSALSA
                     KIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKAFEQFDGFFGSFTNSSQSIRN
                     AIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDSVTSEMSKTLGITQSEALGLI
                     KLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNKLTRTINDNSVSALDAETSVR
                     LLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKAATIGKTERETAKYAATLLAT
                     TDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTADAKAAREAEAAAKKSAQAKK
                     QVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGAAATKEQREEAEKLAQSIYGA
                     TAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILYPQKIMEAEAARAAIEEQYRQ
                     QRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGLLNGTMSVRDAFASIANTILN
                     SVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKALAASFAPAASMVSLATQGANA
                     IPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGENNQPEIFKASNGMQYMIPGQ
                     SGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASANQAAVAKALSNQIKASVRLE
                     LQSQMRAGGVLAR"
     gene            2441..2770
                     /locus_tag="PXB44_002319"
     CDS             2441..2770
                     /locus_tag="PXB44_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282749.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2770..3240
                     /locus_tag="PXB44_002320"
     CDS             2770..3240
                     /locus_tag="PXB44_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5282750.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3248..>3753
                     /locus_tag="PXB44_002321"
     CDS             3248..>3753
                     /locus_tag="PXB44_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5282751.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSLTRETATFTLA
                     LPIETDNA"
BASE COUNT         1248 a          761 c          877 g          867 t
ORIGIN      
        1 tggcggagtt gtaattaccg cagatctaga atttgaaaat ctgctaagag atactcaaaa
       61 agttgataaa gcgatggatg acatcggaaa atcaacatta aaagcagaaa aagggctgaa
      121 agggctagaa acgcaggcaa ccaaaacggc gatagcagta aatcaagcca caaacaaaat
      181 gaaaaatatc cgtggagtag caggtcaact tggttatcaa attcaagaca tctcaataat
      241 gcttcaaatg ggttctaacg cttcaaccgt tattgctcag caagggtcgc aaatcttaag
      301 tattttcggt ccaatgggag ctgttgcagg ggcgatctta gctattagtg gtgctattgg
      361 tggggcgtta ctccctagtt tgtttgattc aactgatgca actgagcttt tggcagcagc
      421 tcaaaaaaat ctcagcgaag tagttactca aacggaaagt ggtgtttccg ccctttctgc
      481 gaaaatcatt aaattagcaa aagtaagcga agatgcagca aaagcaaaaa ttgcagtagc
      541 tatgagtgat gcaaaaatag ctattaaggc agcaggggat tcatcacaaa aggcatttga
      601 gcaatttgac ggcttttttg gatcgtttac aaattcaagt caaagtattc ggaacgcaat
      661 tgacgaatta aaacgctatg aatcaaaagg taaagatgtt ggtgccgtaa taaaagaact
      721 tggaggcaca tatggtggtg caattgccga agttaatgcg ctggattctg tgacaagcga
      781 aatgtctaaa acgcttggta ttactcaatc tgaggctctt ggtttgataa aactgttggc
      841 tcaattagag caagacaaaa gccctgataa tattaaatta ctcgcgatag ctttatctga
      901 cttaagcgat aaaaatgggt gggcgaacga agagctaaat aagctaacca gaacgataaa
      961 cgataattca gtatctgctt tagatgccga aacatcagta agactcttag agtctgctat
     1021 aaaagacttg caaaatgcaa tccaaaactc ccaaggtgca ttggatggca atgtagcaaa
     1081 aatgaatcaa cttgcagatg cggcagaaag aaaagctgca acgataggca agactgagcg
     1141 tgaaactgct aaatatgcag ctacgctatt agctacaacc gatgaagcta aaaaaaatct
     1201 tgattccaca ttaagtagga ttgacgcatc atacgatcaa atcgaagcct atcaagccga
     1261 acaaaaagcc ttacaagata aaaagaccgc agatgcaaaa gctgccagag aagctgaagc
     1321 ggctgcaaaa aaatccgctc aagccaagaa gcaagttgtc gaacagttgc gacaaatggc
     1381 tacacagtac gagattgctg ttttgaggca aaagggaatg catctcgaag ccgtaaaatt
     1441 agaagctggt atgcgtttag gagctgcggc aacaaaagag caacgagaag aagctgaaaa
     1501 actagcacaa agtatttatg gagcaaccgc agcaagtcag aactttaacg ctatacaagc
     1561 ccaagcatct cctgttactg cattagacca acgacatcaa caacagctag ctcagatcga
     1621 agaatataaa atcttgtatc cacaaaaaat aatggaagca gaagctgcga gagctgcaat
     1681 cgaagaacaa taccgccagc aacgaatgga agctcaatgg gaggagtgga agcaagcgag
     1741 cgacggggcg agaatgtttg gtgatgcggt tgatgctgtt ggttcaagtg caacaagcac
     1801 aattacgggg ctactaaatg gcacaatgtc cgtgcgtgat gctttcgcat caattgcaaa
     1861 tacgatctta aatagtgtag tacaaagcct tgtcgaaatg ggtatggctc aagttaagca
     1921 aatgattatg gggcaaactg ctgccaaggc ttctatggcg gcgcaaatgg tgcaagctaa
     1981 agcgttagct gcttcatttg cacccgcggc ctctatggtt tctcttgcca cacagggggc
     2041 gaatgctatt cccgctcaag ctgcgatcac gtcaactatg agtatggctc aagtcgccgc
     2101 ggtgacaggt cgtaaacttg gcggccctgt atcagctaga cagatgtatc gtgtcggcga
     2161 gaacaatcag cccgaaatct tcaaagcgag caatgggatg caatatatga tccccggtca
     2221 aagcggtcgt gtattcagca acaagcagtc gcaaggtcaa ggtggaggcg aaagcaaaac
     2281 agtagctgtc atcgtaaatc aaaccaatca ctttagcagc gatgaagcca gtgcaaatca
     2341 agcggctgtt gcaaaagcat tgagcaatca aatcaaggct tccgtgagat tggaattgca
     2401 gtcacaaatg cgagctggtg gagttctagc gaggtaatca atgcaaacat ttaatttcaa
     2461 ggttgagcaa gactacactg ttcaaaatga gcccagtgtc gtcatactaa aatttgccga
     2521 tggttacgag caaaccgctc ccaaagggtt aaaccacaat ctgaaaaaat accaaggcat
     2581 taccgtaaaa gcgaacaaag cagaagcggt gaagattcag caatttctca atgctcacgg
     2641 tgggtataag aaattcaact ggcgcgatag aacaagcgat gagacggtaa cggttcgttg
     2701 cagaagctgg agctccacta aattaggtgc agtcattgag tttacactta atttcgatga
     2761 ggtgatttaa tgccaaaagc cccccccaaa aaaatggcat cagagttgat aaaactcgaa
     2821 caagatgccc tgattgagct gtgggagata gatctcactc acatctactc tagctcaaac
     2881 ccaaatcagc gcggagagat attccgcttt cacaatggcg tgagccaaac acaacagaac
     2941 atctggtggc aaggcaatga ataccaagca tacccgattg ccgccgatgg ttttgagatt
     3001 tcgggtcaag gaccgtcaaa tcgtccaaca ctcactgtct ctaacctgta cggtgttgtc
     3061 acggtattag tagaagattt cgggcaaccc gtagaggcga aagtcagcag tgaagagact
     3121 gtagatggca atatccaaat gacggtggaa ctgctgaaaa aaatggatca aatcgccgat
     3181 caacgctacc gtagaaatca gttaaatgat ttaagaacag gtggaacatt atctaaatag
     3241 gtgaagtatg ccaattagta tttctaacca aatgaaacta gatcttgcca aacttgagca
     3301 aaatgcaatg ctggacttgt acgaggttga tttgcgtaaa ttgcaagaca aaaacggcaa
     3361 tgctggttca gtttaccgct tttattctgg gctaaatgaa ctcaaaacaa gtattgtctg
     3421 gcaaggtcga acttacgacc cttaccccat cgaggcaaca ggatttgagc gtaacagcag
     3481 cggcccaagc aatcgcccta ctcttacgct atcaaatctg tttggcttag ttacgggtat
     3541 tgctaatcag tttgacgaat gtatcggtgc gattgttcgc cgtcatcagg tttacgccca
     3601 atatttagat gcagtaaact ttgctggagg taatgccaaa gccgatccta atcaggaaat
     3661 catcagccat tttgtgattg agcaactttc aagcctcaca cgagagactg ctacttttac
     3721 gcttgcgttg ccgattgaaa ccgataatgc caa
//
LOCUS       DANLUA010000200         3748 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLUA010000200 DANLUA010000000
VERSION     DANLUA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            142..474
                     /locus_tag="PXB44_002322"
     CDS             142..474
                     /locus_tag="PXB44_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL5282752.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            539..1630
                     /gene="trmA"
                     /locus_tag="PXB44_002323"
     CDS             539..1630
                     /gene="trmA"
                     /locus_tag="PXB44_002323"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL5282753.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1722..1811
                     /locus_tag="PXB44_002324"
     tRNA            1722..1811
                     /locus_tag="PXB44_002324"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Ser,seq:tga)
     gene            1967..2338
                     /locus_tag="PXB44_002325"
     CDS             1967..2338
                     /locus_tag="PXB44_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL5282754.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2335..3246)
                     /gene="xerC"
                     /locus_tag="PXB44_002326"
     CDS             complement(2335..3246)
                     /gene="xerC"
                     /locus_tag="PXB44_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL5282755.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3313..3603)
                     /locus_tag="PXB44_002327"
     CDS             complement(3313..3603)
                     /locus_tag="PXB44_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL5282756.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1107 a          824 c          730 g         1087 t
ORIGIN      
        1 aatatttctc cttaaagagt tttttgcaaa aaatttgcca aatttgaccg cttttttaca
       61 ttaaggcatt gatttattcg ctgtttttgt ttaatgtatg ccgattttat tttccctaat
      121 ttcaaacaac gaggcattat tatggcagac agtttcagcg ttacgcgccg cttttttgac
      181 gataaaaatt atccacgtgg tttctctcgc cacggcgatt acaccattcg tgaatcacaa
      241 actttagaac aattcggtca agcttgcttg gctttagaaa gtggtgaacg taagccggca
      301 actcaagaag aaaaacgttt cgttgcggta atgaaaggcg aagaggtagc agaaactgac
      361 attgaaaaag cgtggttaaa ataccgcaca ttaactagta aaacaaaacg catctacacc
      421 ctttcaggca atgcaggtag cgatgccggc gatgacttca gtgctgctga ataatttcta
      481 atttaaaacc acgaagtaat tcgtggtttg ttttttcatt tgctatttgt ggtaaaaaat
      541 gactcttcct atcgaacaat attccgatct tcttgcaaaa aaaaccgaaa atttgaccgc
      601 tttactcaag ccgtttaacc cgcctgcgat tgaggtattt gcctcaaagc caagccattt
      661 ccgtatgcgg gcagaatttc gagtgtggca cgaggaaggc gagctgtacc acattatgtt
      721 caaccctgaa accaaagctc gttatcgtgt ggatagcttc cctattgcca gtgagctgat
      781 taaccatatg atgaccgcat tattggcgga aattaaaggc aatgaactac tgactcgcaa
      841 gctgttccag atcgattatt taagcacctt aagcggtgaa attgcagtct caatgcttta
      901 ccacaaaagc ctgaatgaag aatgggttga acaggcaaac gccttaaaaa gccgtttaga
      961 gcaacaaggt ttcaaagtgc aaattatcgg gcgtgccaat aaacaaaaaa tcgcactgga
     1021 ttgcgattat gtggaagaaa ttctaccggt acacggcaaa aattatgtgt atcgccaagt
     1081 ggaaaacagc tttacccagc cgaatgcgga aatgaatatt aaaatgctgg aatgggcgag
     1141 aaattgcaca aaaaatagtg aaggcgattt gcttgagctt tactgcggca acggcaattt
     1201 ttcgattgcc ttggcggaaa acttccgcaa agtgctggca accgagatct caaaatcttc
     1261 agtgcaatcg gctcaataca atattgaaaa aaatggcatt gttaacctac aaattatccg
     1321 aatgtcggca gaagaattta cccaagcgat gaacggtgtg cgagcctttt atcgcttaaa
     1381 agggattgat ttaaacgcct acgaatgcaa taccattttt gttgatccac ctcgtgcagg
     1441 gttagatcaa gctacattaa atatggtgca agcgtatgag cggattctct atatctcttg
     1501 caatccggaa acccttgccg agaatttaca acaattaagc caaacccatc ggattgaaaa
     1561 agccgctctt tttgaccaat tcccttacac ccaccatatt gaaagtggcg tttggttaat
     1621 tagaaaataa attgtgctga aaaaaagaac gaataagcag cttttaagat ttattacttg
     1681 acgatgttcg caataatctc tattattgca acgttttcaa cggaggaatg gcagagcggt
     1741 tgaatgcacc ggtcttgaaa accggcgagg gttaacgccc tccgtgagtt cgaatctcac
     1801 ttcctccgcc agtctttaaa cctgttgagc aatcagcagg tttttttatt tcatttgcaa
     1861 gaaaatcaga taaaaccacc gcttgaatcc atcgctataa actggttgaa atcagcctaa
     1921 agttgcttta ggataattgc gattcattat caataactta attattatga aataccttta
     1981 tattatctgt ggttttattt gtattggttt aggcatcatc ggggcagtat taccgggctt
     2041 gccgaccaca ccgttcttac ttttggctct gttctgcttt actaaaggct cggagagaat
     2101 ccgccagtgg tttattcaaa ccaaattata caaaaaatat ctgcaagaat atgatgagaa
     2161 acgggcaatg acgatgaagc aaaaaatcac gattttagcg atttcgtttc cgtttacact
     2221 atttgctttc tttgcactgc cccatatcgg tgggaaaatt gcgttagtag tgttagtgat
     2281 aattcaatat tggtatttct tctgcaaaat gaaaaccctt gaaccggaga atgcctaatc
     2341 ttcctgcttc cgccttgctc ttgggtgggc agagtcataa attttagcta agtgttggaa
     2401 atccagatgc gtataaattt gagtggtgga caggctacta tgacccaata attcctgaac
     2461 cgctcgcaaa tcaccgcttg cctctaacat atgagtcgca aaagagtgac gtaatttatg
     2521 tggatgcaga tggctttcca gcccttgtcg ctcaccccat ttcttcattg ccaactgaat
     2581 ggaacgatga gataaccgcc cacctcgctt attcagaaac accgcattat cttgcggctt
     2641 aaattgcaaa cgcatttcta accagcgatt taacgctttt aacgcttgcg agccaatcgg
     2701 cacaattcgc tctttgctac ccttaccttg cacccgtact tctcgtgccg ataaatccat
     2761 atcgcctaaa tccagccctt gtaattccga taaacgcaag ccggaactat acatcagctc
     2821 catcatcgca atatctcgaa tatcgctcgg ctcatcactc tcaatcgcca gcaactgccc
     2881 gacctgctcc gcatcaatat ttttcggcaa atgcttacct actttcggcg atttcacccc
     2941 taaagtcggg tttgccgaca ttcgcccgat tttaatcaaa tagacaaacc attgccgtaa
     3001 cgccaccaag cgtaagccga tacttttcgc actcaaccct tgcttatggc tttggcttaa
     3061 catccaacgc accgagctac tttcgacctc ttcccaattt tcaatgcctg cttgcgaaaa
     3121 aagctcgctc accgccatta attggcgttg ataattgctt aaggtatgcg ggctaacttg
     3181 cttttcaatg cgtaagaaat cccaataggg gcgggtttgt tggaaaagag ggttggaagt
     3241 ggtcataaac gtaataacaa gcggttaatt tttaggaaaa tgttgcaagg gcaaaagatt
     3301 tgccctttga gattaggcta gccccaactg accgatacgc acgccaacat taccaagtgc
     3361 aacagggctt aaatattcgc cgagaaattc aaacaattca ttcacatcag taaaactgcg
     3421 atgagttttc acttcttgct ccactttacg agtaaattca taatgtacat tttcgccgtt
     3481 aataaaggct tccagcgtta aatatacggt ttcaaaatag tggcttggcg agccttcaaa
     3541 gcccggatca ctcactcgaa cattccaatg attactaaat aaaacttcgg tttttactga
     3601 cattgtgttt tccttcttcc tataagtacg tacattattt taatcaagat agattgaatt
     3661 tgccactttt cctagtcaga atcgaggcaa atcttatttg gtttgtcctc ttatatcttt
     3721 ccctcaaaat atgatcttgg tcaaaaaa
//
LOCUS       DANLUA010000201         3715 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLUA010000201 DANLUA010000000
VERSION     DANLUA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(64..2628)
                     /locus_tag="PXB44_002328"
     CDS             complement(64..2628)
                     /locus_tag="PXB44_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL5282757.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2752..3450
                     /locus_tag="PXB44_002329"
     CDS             2752..3450
                     /locus_tag="PXB44_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL5282758.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3426..>3715
                     /locus_tag="PXB44_002330"
     CDS             3426..>3715
                     /locus_tag="PXB44_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282759.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILNIENKIISLNSNHTNTLPFAEPKKIAQLLA"
BASE COUNT         1131 a          822 c          617 g         1145 t
ORIGIN      
        1 ttattatcgc aagaaatccc cctatttttc aacttttaag agcattatcc ttgtaataaa
       61 ttattagaat aattcaccgc tttttagcac ccctggtgga ggtaatccta aacgtaacgg
      121 cgcagattct gttttttgag gcgctttttc agtataagct ctctctacaa tataacgttt
      181 tgtaggctca gtacccttaa taaaatactc tttacgtcca tttcctaaga aaccactatt
      241 agggtcaata ttaacctcaa taatattttt tggaagggta cttttttcga caggcttatc
      301 tgacaatgca actttcatat aatttgtcca agctggtaaa gcagtactag aacctgaagc
      361 acctctacct aatactcttt tattatcatc aaagccaacg tacacaacag tagaaatatt
      421 agcaccaaat cccgcatacc aagttgcctt ggcattattg gttgtaccag tcttcccgcc
      481 cacatccgct cgttttatcg tattgagcat tttatagctt gttcctcgcc agccatattc
      541 aggctcacca gtaattgctg tagctaaagc actacgcatt aaaaaagcga gttcatcact
      601 aataacatga ggagcgtaac gtaacccttt gatatgatga gaagattctg ccattaagct
      661 cggcgcattt ttctcgctat tgttcgcttg taattcaggt tctgaatcgc caacatcttc
      721 cacaacatcg tcgtcatcat tattcgagga attcgctata gtttgtacac cgacttgagc
      781 ttcatcatga attttgactg attcaaaata tttaggctca gggtaaacaa ctgggctatc
      841 gcaactatta caagctacta caggatttgc ttgatataac tcgtagcctt gggcatcaat
      901 aatacggtca atgatatatg gttcaatcaa ataacctcca ttattaaata ctgcataagc
      961 tcgagccatt tctaatggcg taaaagatgc agcacccaat gcaagagatt ctgttgcaac
     1021 atactgattt cggttaaagc caaaacgttg taagtagtct gcaacataat caatccctgc
     1081 catttgtaat atacgaatag ccaccatatt tttcgattta cctaatgcaa cacgagttct
     1141 caacgggccg ccataaaccc catcggcatt tttaggtcgc cactcttttt gccctttctt
     1201 tttaatgaca ataggagaat cgctaattgt tgttgaaaga cttagccctt tattcattgc
     1261 cgctgtgtaa ataaacggtt taatcgcaga gcctacctga actaatgact gagtcgcacg
     1321 attgaaacgg ctttgttcaa agctaaagcc tccaacgata gattcaatgg caccgttttc
     1381 actattaatt gacactaggg ctgaatttac ctccggaatt tgtcctaaag cccactccct
     1441 atttttattt tgacgtaccc aaatttgttc acctgctttt aaccctttag gtgcaaaaga
     1501 agcatttgta cgttttaaaa cagagatatc accatttgcc aataaaattg tatattttga
     1561 tttatctgct tgcattacaa cagctggtgt aaacggctct gattttggca gcttacttaa
     1621 atgatcgata attttctcat cgtcccaagc agaactacct gtccataatt tttcagcgcc
     1681 tcgccaacca tgacggcggt cataatcaat caaattttca cgtaacgcat cttgtgctgc
     1741 tttttggtct gctgaaagta ccgtggcgta cacttttaag cctttgttat aagcaatatt
     1801 ttctccatag cgtttcacaa tttcttgacg aaccatttct gtcacataat ccgctcgaaa
     1861 ttctaatgtc gcaccatagt agttagctac tactggctct gctttagctt tctcatattc
     1921 ctcttttgta atatctccgg tttctagcat tctcccaagc actacattac gacggtcttc
     1981 cgcccgtttt actgaataaa gtggattcat cgttgaaggc gcttttggaa gccctgcaat
     2041 aattgctatt tcagaaaggg ttaaatcttt caaagattta ttaaaataag ttttagccgc
     2101 agcggcaaca ccgtgagaac gataccctag gtaaattttg tttaaataca gctccagaat
     2161 ctcattttta ctgagaacct gttcaatttc taaagctaaa attgcctctt tggccttacg
     2221 ctctaaacta cgttcaggtg ttaagaaaaa gtttttcgcc agctgctggg tgattgtact
     2281 tgcgccttga gtatctccct ggctagtgcg ccatattgca cgcataatac ctttaggatc
     2341 gatcccttta tgctcgtaaa aacgtgcatc ttcggtagca agaatcgctt ttactaacat
     2401 cggtggaata tcatcaagct tgaccggaat acggcgttct tctcctactt caccaattaa
     2461 tttaccatct aaggtaaaaa tctgcatagg ctgctgtaac tctacatttt tcagcgttgc
     2521 cacatcaggc aaatcagatt tgaaatgcat gtacagtaaa ccaccaatta ccccacctaa
     2581 aaccaagagt gaaagtaaag cactaaatat tatttttgcg atccgcatcg aaaaaatctc
     2641 ttttggttaa tgacgaaaca taaatttaaa gccaatatta ggtcactaag tataagctaa
     2701 aaacgcttac ttcgcataac taattgcaaa actttcctta ctatacttac tatgaaatta
     2761 cttaaagcac attcaaaaac accttcaaac cgtatcatct taggcttaag tgaagatgaa
     2821 caacgttact gcttagtcaa aaataaagca gaaaaattca ccgttttttg gcaagccaag
     2881 cctgcgagca tcgaagagct tatcggtaat ctcaccccac aagaacaagc aatgatgacg
     2941 ctggttcgcc cgatccctta ccaatacatt tggcgaaaaa ccgtttttat ggctaacacc
     3001 ctcaatgaag ctcaattaca ccgtaaggta attcacattt taaaaaatga gcagccactt
     3061 gcattggatc tactcaatat tgactatcag cgatttccaa gcagcaacga taatctggat
     3121 aaagtggtta tctacgccgt gcgaaaaagt tataccgaga atttgagcca atttaacagc
     3181 atcttagatt gtgagctgca ttgctatgca cgggctataa ttcatttaac acaatcccaa
     3241 aataatcagc ctgcgccctg tttttcattt aaacagaagt acttccaatt taccgagacg
     3301 gaattactga tattgcaaac agaacctgag gattgtattc atctatctga tctcaaaatt
     3361 gaccatcttg aaatcgagtg tgagcaagag aaacagctat attgcctcgc cttaggagca
     3421 agtttatgga atggcaaggt attgatttaa gtgctaatcg aaaaaataac tggtttgccg
     3481 gcagtgataa aggcatcaaa aacgtgattt atacactttt attatctgcc ctcattagct
     3541 tggctctgat ttttctctat ttgcaaaaaa atcaagaaat tcaaccgctt gcacagcaat
     3601 tagagctggt aaaaaccgat atattaaata ttgaaaataa aattatcagc ttgaatagta
     3661 atcatacaaa caccttgccc tttgccgagc ctaaaaaaat agcacaacta ctggc
//
LOCUS       DANLUA010000202         3670 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLUA010000202 DANLUA010000000
VERSION     DANLUA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..372
                     /locus_tag="PXB44_002331"
     CDS             <1..372
                     /locus_tag="PXB44_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL5282760.1"
                     /translation="NPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVISR
                     HLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEFEQ
                     VIGLLSQYTGCLSLVGGRKFA"
     gene            439..879
                     /locus_tag="PXB44_002332"
     CDS             439..879
                     /locus_tag="PXB44_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5282761.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRYAQEKGYNRVYWVTDKNNHQAQQLYNKLANDSGIIQYRKNF"
     gene            909..1976
                     /gene="hisC"
                     /locus_tag="PXB44_002333"
     CDS             909..1976
                     /gene="hisC"
                     /locus_tag="PXB44_002333"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5282762.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            2161..3249
                     /gene="hisB"
                     /locus_tag="PXB44_002334"
     CDS             2161..3249
                     /gene="hisB"
                     /locus_tag="PXB44_002334"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL5282763.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
     gene            3344..3628
                     /locus_tag="PXB44_002335"
     CDS             3344..3628
                     /locus_tag="PXB44_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282764.1"
                     /translation="MIREVIDFIKNNKWYVLLVVLLLGLYFYQFHGGLSSDSEKWDHF
                     GSYIGGIFSAVTLLSVLHGMQLERKRFKEQKEEFENDKRVLEKRRRKEDF"
BASE COUNT         1113 a          770 c          853 g          934 t
ORIGIN      
        1 aacccgattg aaatttacca aagccaagac ggcaaaaccc aagtttccgt gcagtttgat
       61 aaggaaacgg tgtggctaag tttacagcaa atggcagata tttttggacg agataagtct
      121 gttatttctc ggcacttacg aaatatctac caagaaggtg aactggagcg tgaggcaact
      181 gttgcaaaaa atgcaacagt tcaaattgaa ggtgctagag aagtcgttcg cctaaaagac
      241 tatctggtga aaggctatgc ccttaacgaa cgccgtttgc aggaacgagg tatcgagttt
      301 gagcaggtga ttggtttgct cagtcaatac actggttgtc ttagccttgt tggtggcaga
      361 aagtttgcct gaacaaaaag aactgatgat taaactgatt gaacacttta ttttattaaa
      421 agcataacag gaaaaaatat gagtattaat atttcgccgt tagcctccaa aaatctcact
      481 gaatggaaac tattgtggca acaatattta gatttttatc aagtgaattt agcagatgat
      541 attattcaat caacttggca gaaagtttca gagagtgaaa atgtaaaggg atttggggct
      601 tttgttgata acagaatggt tggttttgtt catattgtga ttcacccaaa tacttggaat
      661 ttaactgaat gctgttattt ggaagattta tttgttttag ctgaatatcg caaccaaggc
      721 ataggaaaag aattaattga atatatttat cgatatgcac aggaaaaagg atataaccga
      781 gtatattggg taacagataa aaacaatcat caagcacaac agctttataa taaattagct
      841 aatgatagcg gtattattca atatcgaaaa aatttctaaa ctatgtataa attatacagg
      901 aacacaatat gacaatttca caactttccc gtaaaaacat tcaagctctc accccttacc
      961 aatcggcacg gcgtttgggg ggtagtggcg atgtgtggct gaatgccaat gaatatgccc
     1021 tttcgccaaa ctttgattta accgaccgca cttttaaccg ctaccctgaa cctcaaccgc
     1081 aggcggtgat tgaaggttac gcccgatatg caggcgttgc acctgaaaac gtgctggttt
     1141 cccgtggggg cgatgagagc attgagctga ttatccgagc cttttgcgag gcaaacgaca
     1201 gcattctcta ctgcccacca acctacggaa tgtacgccgt gagtgccgac acctgtggca
     1261 ttgcgaccaa aactgtgcct ttaaccgccg atttccagtt ggatttagcc caaatcaaag
     1321 ctaacctcga gggcgtgaaa gtggtgtttg tgtgcagccc gaacaacccg acaggcaact
     1381 taatcaagcg gtcggatttg ttcgaacttt tgcaaatcac cgcaggcaaa gcgattgtgg
     1441 tggtggacga agcctacatc gaattttgcc ctgaagctcg cttggtaagc gagctggcga
     1501 atttcccaca tttggcgatt atccgcaccc tatccaaagc ctttgccttg gcgggtttgc
     1561 gttgtggctt taccttggca aacgctgagc tgatcggcgt gttacaaaaa gtgattgccc
     1621 cgtatccgct gcctgtgcct gtttccgaca tcgccgccca agccctctcc ccacaaggca
     1681 ttgagcagat gaaaacccgt gtggcggaag tcatcaccct gcgtgccgaa ttgcaaaaaa
     1741 atctggaaaa cttaccgctt gtagaacaag tttttgacag cgaagccaac tacctcctat
     1801 tcaaatgcca agacggacaa aaagtcttca aagcactttg ggagcaaggc attattttgc
     1861 gtgaccaaca caaagcgtta ggattgcaaa attgcattcg gattacggtg ggggcgagag
     1921 aggagaattt gagggtggtt gaggcgatta cagcactgag ccaatttcag agatagaatt
     1981 taacgagcag tattttaaga gacatttata gcctagcacg gcatagccgt actaggttac
     2041 gcataagtca gtccctttgg gactgaatac agagaggcaa agtctctcac taatgcttaa
     2101 ccccatatgc cttggcgtgt ggggagagta atgaataaga tacagataca ggagaaacca
     2161 atgcaaccca ccctttttat cgaccgtgac ggcacgttaa ttgatgagcc aaaaaccgat
     2221 tttcaaatcg acagcttgga aaagctgaaa ttcgagaaaa acgtgattcc tgccctgctc
     2281 aaactcaagg gcaaataccg ctttgtgatg gttacgaacc aagacggctt aggcacgagt
     2341 tcgttcccac aggcagattt cgacaaaccg cacaatgcaa tgatggcgtt gttcaactcg
     2401 caagggattg aatttgatga tgtgctgatt tgtccacaca agcccgaaga cggttgcgat
     2461 tgccgcaaac cgcacaccaa actgctacaa aaatatatcg accgccaact gttcgaccca
     2521 gccaacagct ttgtgattgg cgaccgtgct accgatgtgc aactggcgga aaaccttggc
     2581 attcgggcgt tgcaatatca ccctgaaaaa ctgaactggg atttgattgc tgaaaaacta
     2641 ctgggcgaag ccgtcaccaa cattggcgag cgaccaaagc gctttgccga agttatccgt
     2701 aaaaccaaag agaccgacat caaagtgcaa gtgtggctgg acgaaacagg caacaacgaa
     2761 atcagcaccg gcgtgggctt tttcgaccat atgcttgacc aaattgccac ccacggcggt
     2821 tttagaatga acatcaccac caaaggcgac ttgtggattg acgaacatca cacggtggaa
     2881 gacaccgccc tcgccctcgg caccgcatta aaacaagcga ttggcgacaa acgcggcatc
     2941 gcccgtttcg gcttcgtgtt gccgatggac gaaagccgtg cggaatgtgc gatggactta
     3001 tcaggcagac ctttcatcaa gttcaaagcg gatttcaaac gcgacaaagt gggcgatttc
     3061 agcaccgagc tcaccgaaca cttcttccaa tccatcgcct tcacgatgct cgccaccctg
     3121 cacatcaagg caaaaggcga caacgaccac cacaaaatcg aaagcctgtt caaagtcttc
     3181 ggcagaacac tcagacagtg cattcgtatt gaagggaatg aactaccaag cagtaaaggt
     3241 gtattataat caccgtaggg gcgggtttta acccgcccga aagtgcggtt gaaatttgca
     3301 aagtttttgg ggattttgac cgcttgttat aaaggatata ttgatgatta gagaagttat
     3361 tgactttata aagaataata aatggtatgt tctgttagtt gtattgttat taggattata
     3421 tttttatcag tttcacgggg gattatcctc agatagtgaa aaatgggatc attttggtag
     3481 ctatattggt ggtatatttt ctgctgttac tttattgagt gttttgcatg gaatgcagtt
     3541 ggaacgtaaa agatttaagg agcaaaaaga agaatttgaa aatgacaaaa gagtattaga
     3601 aaagagaaga agaaaagaag atttttagcg tacatttttt atgatgttag agcagcataa
     3661 tcttaaaatt
//
LOCUS       DANLUA010000203         3652 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLUA010000203 DANLUA010000000
VERSION     DANLUA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            94..456
                     /locus_tag="PXB44_002336"
     CDS             94..456
                     /locus_tag="PXB44_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL5282765.1"
                     /translation="MKPTNKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            493..2136
                     /locus_tag="PXB44_002337"
     CDS             493..2136
                     /locus_tag="PXB44_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL5282766.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2343..3212)
                     /locus_tag="PXB44_002338"
     CDS             complement(2343..3212)
                     /locus_tag="PXB44_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5282767.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3222..>3652)
                     /locus_tag="PXB44_002339"
     CDS             complement(3222..>3652)
                     /locus_tag="PXB44_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5282768.1"
                     /translation="KSLPPVFARLCKKLKSLDLVNLELGWQELENGIRMNVMSFETTP
                     AEGKKAELHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDYQLTAEIDDKNNL
                     ILKPNMFAIFLPYEPHKPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1164 a          702 c          656 g         1130 t
ORIGIN      
        1 tggctatcat attatttttt ccttaggttc tttgaacatc accattcttt cagtacaata
       61 gttctctaaa ctcttttatt tattttattt actatgaaac caaccaataa agcagatttt
      121 caacgtgtga tcaatgcagc tggctactct atgaaggggt taaaagccgc ttatattaat
      181 gaagccgcct ttcgccaaga aatttggtgc gctactattt tattccctct agggcttatt
      241 ctaggcgaaa ccaatattga aaaagcatta ttagtaggca cagttttact tgttttagtt
      301 acagaactac tcaatagtgc ggttgaatct gttgttgacc gcattggggc tgatttccac
      361 gagctttctg gtagagctaa agatattggc tctgcagctg tatttatggc gatggtttta
      421 ctcgctataa cttggatatt aattttgttt tgttaactct atttttcaac caacactctt
      481 actgaggtat ttatgcgctt aaaaaaaaca ttactttcgg tagcactact tagtgcaaca
      541 tcagcaatgg cctacgagac aggtaaaaca tatcgtttta cggttttaca tactaacgat
      601 atacacggtc acttttggca aagtgataaa ggcgaatacg gtctcgctgc tcaaaaaaca
      661 ttggttgata atattcgcaa agaggtcgca gaaaaaggtg gttcggtatt aatgctgaat
      721 gcaggtgatg cgaatacagg cgtgccggag tcagatatgc aaaatgcacg tcctgatatt
      781 gaaggaatga atgcgattgg ttacgatgca atggttctag gtaatcacga atttgattac
      841 ccactgcaaa ttctctctat gcaagaaaaa tgggctaaat tcccctttat ttcggccaat
      901 gtcattaata aaaaaacgga taaacccatt gtaaaacctt acgtgatgtt agaacgaggc
      961 ggactaaaaa ttgctgttat cggattaaca accgaagata ccgcaaaatt aggtaatcca
     1021 gatgtaacgg aaaatgcctt tttcaaaaac cctattgaaa cagcaaaaaa cacattagct
     1081 gaaattaatc aaaaagaaaa acccgacgta cgtatcgctt taacccatat gggttactat
     1141 tttgacggta aacatggtac taatgcacca ggtgatgtaa caatggcccg taccttagaa
     1201 aaaggagcgt tcgatttaat cattggcggt catacccacg atactgtttg tattgatgaa
     1261 aaaggtcaat tcaaagccaa atatacgccg ggtgaggaat gtaaaccaga cttccaaaac
     1321 ggtacttgga ttgtgcaagc cggcgaatgg ggcaaatttg ttggacgggc tgattttgag
     1381 tttaaagatg gaaaagctac cttggtaaaa tatgaattaa ttccggtcaa cttaaaaaaa
     1441 accattaaaa aagaagacgg tactaaagaa taccgacttt accagcctga aatcaaagcc
     1501 gatgaagcaa cctatacctt attgaaaaaa tatcaagatg agggcgataa aaagcttaat
     1561 attgaagttg gtcgtgtgaa agggttatta gacggcaaac gtgagcatat tcgcttccaa
     1621 caaaccaatc tcggtcgttt aattgcacaa tcgcaaatgg aaagggtaaa agccgatatc
     1681 gggattatga actccggcgg tattcgtacc tcccttgaag aaggtgtaat aacttataaa
     1741 catattctga ctgttcaacc tttcggtaat atgattgcaa cggtggattt aaaaggacaa
     1801 gaattgttag ataccctcac cactattgct ctgaaagaag ttgatactgg cgcttatcct
     1861 caatttgccg gcatttcaat ggtagtcgac cgtacagcaa aaactatttc tgatgttaaa
     1921 atcggtggca aaccttttga taaagagaaa acttataaag tttctctgcc tgactatgtt
     1981 gcggctggtg gcgatggcta cccattactg aaaaaacacc ctagctatgt caatacaggc
     2041 tttattgatg ccgaaatgct gaaaaaattc ttcgaagaaa ataaaattat tgaggcagat
     2101 aaatacgatc caaaaaacca gatcattttc aaatagagct atagaaaatg aaactcactc
     2161 tggcagtctc tgctcaaaag agctgtggct gcctgtagat tgttttctta ctttcaagtg
     2221 ttataggaaa tcgataaaaa atccctacca aattaattca tcaccatatg atgaaaatta
     2281 aaaaacctct ggcaatactt tgccaaaagt ctttgacttt tatagggtaa tatcactgct
     2341 tattagcctt taacacgttg ttccaaaatc acattcagtg ttttttgttt ctgtttcaac
     2401 gcattttcgg gttcggcttt aactagtaag gtataaatta aatcaagaac gaaaagttgt
     2461 gacattttag taccgataga atctccttgc atatgcccct gtcgattacc attaattaac
     2521 acataattag cctcttccgt gacgggggaa cgtaaattgt gtgtaatcgc aaccgttgtc
     2581 gctttatttg cacgagaaaa acgtagtgat ttcacaattt cttcagaata gcctgaatga
     2641 ctaatcccta taactaaatc cccctctttc accaatgccg cctgcatata cataaaatga
     2701 ttatttgtga ctgcatcggt ttgtagccca atacgcatta atttatgttt tgcatcttct
     2761 gcggttaacc cagaagaacc cactccaaat aaaaagatcc gttttgcctt acgtaattct
     2821 tcaacaactt tttccaacac gttaaagtca agcaaattaa tagtctcttc aataacgtta
     2881 tctaaactca ctttgagctt ctcagcaata tctttcgatg tatccgattc tgaaacatcc
     2941 gtatcaagca atactctatt atcttgattt tgcgtagcca actctattgc aagctctaat
     3001 ttaaagtcgg tataaccttt aaaacctaat gtacgacaaa aacgaataaa agttgcttca
     3061 ccaacatcta attgtttggc cacttcagat aatgaacatt ggctaagtaa ttccggctga
     3121 gataaaatag cagcagctat tttcttctct gttttggtta aactattttg tagcgagcta
     3181 attgtattaa gaatttttcc ccgactagac atagtagcct cctaaatcaa ttcaacaggt
     3241 actttcacca ccagtttttt caatgttttg ttgccattca ccgaatttcc aggcttatgt
     3301 ggttcataag ggaggaaaat agcgaacata tttggcttga ggatcaagtt atttttatca
     3361 tcaatctcag ctgttaattg gtagtcatct tcatctcgat attcatcata cttagataaa
     3421 tctggctggc ttaacccgta ttcaatcatt tcttcgccgt ctatcaataa ttgaatatcg
     3481 ataaattttc ggtgtaactc tgcttttttc ccctcagctg gcgtagtttc aaatgacatt
     3541 acattcatgc gaataccatt ttctaattct tgccagccta attccaaatt aactaaatct
     3601 aaacttttta attttttaca aaggcgagcg aatactggtg gaagagattt ta
//
LOCUS       DANLUA010000204         3635 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLUA010000204 DANLUA010000000
VERSION     DANLUA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..573
                     /gene="nfuA"
                     /locus_tag="PXB44_002340"
     CDS             <1..573
                     /gene="nfuA"
                     /locus_tag="PXB44_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL5282769.1"
                     /translation="SISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAECGVSYCPP
                     NAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNAKMRKVADD
                     APFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSMVDVTLKEG
                     IEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(741..2378)
                     /gene="pyrG"
                     /locus_tag="PXB44_002341"
     CDS             complement(741..2378)
                     /gene="pyrG"
                     /locus_tag="PXB44_002341"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL5282770.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            2642..>3635
                     /gene="ansB"
                     /locus_tag="PXB44_002342"
     CDS             2642..>3635
                     /gene="ansB"
                     /locus_tag="PXB44_002342"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL5282771.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQL"
BASE COUNT         1103 a          804 c          714 g         1014 t
ORIGIN      
        1 tcaatatcag atgcagcaca agcccatttt gccagactat tagaacagca agaagaggga
       61 actaatatcc gtatttttgt ggtcaatccc ggcacaccgg gggcagaatg cggtgtctct
      121 tactgcccac ccaatgcagt tgaagagacg gataccgaat ttaaatttaa ccgtttctct
      181 gcatttgtcg atgatattag cttaccgttt ttagacgaag cggaaattga ttatgtaacc
      241 gacccaatgg gttcacaact taccctcaaa gcccctaacg ccaaaatgcg taaagtggct
      301 gatgatgcac cgtttatcga gcgtttagat tatgtgattc aaacccaagt caatccacaa
      361 cttgccagcc acggcggtaa agtgacctta attgaaatca ccgaagataa atatgccgtg
      421 ctacaattcg gcggtggttg taacggttgc tcaatggtgg atgtgacctt aaaagaggga
      481 attgagaaac agcttctggc aatgttccct gatgaattag ttggtgtgaa agatattacc
      541 gaacaccaac acggtgaaca ttcttactat taaaaatact aaaagggata aatcattgat
      601 ttatcccttt cttataatta atgagcaagc ggtcaaattt actcgctttc gctcgcactt
      661 cgtgccatca gcaaagccga tgttcaaacg caagcgtttg tacaaaaaaa tgagtaaatt
      721 cgaccgcttg ttaagtttga ttaagcctta cgactctctt ggtgatcttt cgcagctttc
      781 acaaaacctg caaataatgg atgaccatca cgcggagttg aggtaaattc cggatggaat
      841 tgtgctgcta taaaccaagg gtgatttggc acttcaataa tttccactaa tttacgatct
      901 gccgataaac cgctcacttt taagcctgcc gcttcaattt gtggtaacag cttattgttt
      961 acttcgtaac ggtggcgatg acgttcaaca atcgtttccg caccatataa ctcacgagct
     1021 tttgagcctt caattaaatg gcattgttgt gagcctaaac gcattgtacc acctaaatct
     1081 gactcatcgc tgcgggtttc cacattccct tccgcatctt gccattcggt aattaagcca
     1141 atcaccggct gtgagcaatc tttatcaaat tcagaagaat ttgcctcggt taaacctgcc
     1201 acattacggg catattcaat tagggcgatc tgcataccta aacagatacc taagtaagga
     1261 atcttgtttt cacgggcata ttgtgcggtt cgaattttgc cttccacgcc acgatagcca
     1321 aagccgcccg gcacaagaat gccgtctaag cctgccaata actctgtgcc tttggtttca
     1381 atatcttgtg aatcaatgta tttaatattc actgttaaac gatgcgttaa acctgcgtgt
     1441 ttcaaagcct cattcaccga tttatacgca tcaggtaatt ccacatattt tccgaccata
     1501 ccgatagtca cttcgccggt tgggtttgct tggcagtata gcacttgttc ccactctgaa
     1561 agatcggctt ctttgcaatc taaacggaaa cgatcgcaca caaaggaatc taacccttgc
     1621 gatttcaata attccggaat gcgataaatc gaatctacat ctttaagcga aataaccgca
     1681 cgctccggca cattacagaa aagggcgatt tttttcttct cattttccgg aatagcacga
     1741 tctgaacggc aaattaacac atccggctga ataccgattg aaagcaactc ttttactgaa
     1801 tgctgagtcg gcttagtttt cacttcccct gcggttggaa tataaggcac taaggttaaa
     1861 tgcataaaca aggttttttc acgaccgata tccaccgcta attggcggag tgcttctaag
     1921 aatggcagtg attcaatatc ccccaccgtg ccacctactt ctacaatggc gacatcacgc
     1981 cctttaccgc cttctaacac acgggcttta atctcgttgg taatatgcgg aataacctga
     2041 atggttgcac ctaaatagtc gccacggcgt tctttacgca acacttcaga gtaaatttta
     2101 ccgctggtaa agttgttggc tttgctcatt tttgaacgga taaaacgctc gtagtgacct
     2161 aaatccaaat cggtttccgc accgtcttcg gtcacaaata cctccccgtg ctgagttgga
     2221 ctcattgtac ccggatcgac attgatataa ggatcaagct tcataatggt tacatttaaa
     2281 ccacgagctt ccagaataga tgctaaagag gctgccgcaa tacccttccc taaagaagat
     2341 acaacaccgc ctgtgacgaa aatataattc gttgtcattg tgattgcctt tgctgattga
     2401 ttaggttgga aattcagaat atcagtttac ttcgtaaaaa agaaactatc aggacgggaa
     2461 agtagtttac atcaaattac atttttttgc taatggaaaa tcggcaagca gtcagattaa
     2521 ttcttttttc aataatgccg actaatctta attaatacac tattatttgc gatagatcag
     2581 aattaaccta catgtttata gatatcataa tacctgtttt tctaatactg gagacgatac
     2641 aatgaaaatc aaaaaattac tactgccaac tctgttaagc attgctacta tgccaattca
     2701 agcagccgac ctacctaata ttacaattct tgcaactgga gggacaattg ccggtagcca
     2761 aaaaaatacg gcaacctcag cttatcaagc tgggcagcta aatattgaaa cactcataga
     2821 agccgtcccg gatattaaaa aattagcaaa tatcaaagga gaacaaatcg ttaaaatcgg
     2881 atctcaggat atgtctgatg aagtatggct aaaactggca aaagcaatta atcaacaatg
     2941 cccaaacaca gatggcttcg ttattacgca cggcacagac actatggagg aaaccgtata
     3001 cttccttgat atgactgtaa aatgcgaaaa acccgtagta ttagtggggg ccatgcgacc
     3061 agcaactgaa aaaagtgccg acggcgtatt aaatttatat aactcagttg ttgtcgccaa
     3121 agattctaaa tctagtggca gaggggtgtt ggttgcaatg aatgatattg tattgggcgc
     3181 tcgcgatgtt acaaaaacca gtactacatc agtgcaaaca tttaactcac caaactttgg
     3241 cgcactaggc tatatccata atagcaaagt tgattatgag cgctctcctg aaagtaaaca
     3301 caccgtaaac acccctttca aagtagatga attaacggaa ttgccaaaag tcggcattat
     3361 ttatgcttat tcaaatatgc caaccgaacc gcttcaagca ttattagatg cgagttatca
     3421 aggcatcgtg gttgcaggag ttggcaatgg caatatgaat gcggctaatt tggctttact
     3481 tgaagaagca gctaaaaaag gaattaccgt ggttcgctcg tctcgagtgc caaccggata
     3541 tactacccga gatgcagaaa ttgatgatag caagtatggt tttattgctt ccggcacatt
     3601 aaatcctcaa aaagctcgag tattactgca gctcg
//
LOCUS       DANLUA010000205         3497 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.207, whole genome shotgun sequence.
ACCESSION   DANLUA010000205 DANLUA010000000
VERSION     DANLUA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(45..215)
                     /gene="rpmG"
                     /locus_tag="PXB44_002343"
     CDS             complement(45..215)
                     /gene="rpmG"
                     /locus_tag="PXB44_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL5282772.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(227..463)
                     /gene="rpmB"
                     /locus_tag="PXB44_002344"
     CDS             complement(227..463)
                     /gene="rpmB"
                     /locus_tag="PXB44_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL5282773.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(695..1432)
                     /gene="radC"
                     /locus_tag="PXB44_002345"
     CDS             complement(695..1432)
                     /gene="radC"
                     /locus_tag="PXB44_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL5282774.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            1582..2784
                     /gene="coaBC"
                     /locus_tag="PXB44_002346"
     CDS             1582..2784
                     /gene="coaBC"
                     /locus_tag="PXB44_002346"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL5282775.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2784..3101
                     /locus_tag="PXB44_002347"
     CDS             2784..3101
                     /locus_tag="PXB44_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282776.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            3098..3280
                     /locus_tag="PXB44_002348"
     CDS             3098..3280
                     /locus_tag="PXB44_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5282777.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
     gene            3267..>3497
                     /locus_tag="PXB44_002349"
     CDS             3267..>3497
                     /locus_tag="PXB44_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5282778.1"
                     /translation="MKKYNIVFSADKNYLPYLEIALKSLLAHHNHLSIFILSTGDISH
                     HWIETLRPYLENRYSTLRLGYLDSNNLANFNSN"
BASE COUNT         1054 a          719 c          707 g         1017 t
ORIGIN      
        1 atgaaaaacc cagcattcgc tgggttttaa atcttaaaac aaaattattt aatttttgct
       61 tctttgtaga taacgtgttt acgcacaact ggatcaaatt ttttgatttc cattttttca
      121 ggcatattac gtttgttttt atctgttgtg tagaagtgac cagtttctgc tgtagaaact
      181 aaacggattt tctcacgagc gcctttagct gccatttttt agctccttag attttttcgc
      241 cacgagcacg gatttcagct aatactgcat caatgccttt tttatcaata atacgcatac
      301 ctttcgcagt taaacgtaag gttacgaaac ggttttcgct ctcaacccag aaacggtgag
      361 tgtgaaggtt tggtaagaaa cgacgtttag tcgcattcat tgcgtgtgag cggttgttac
      421 caactgctgg acgcttgccg gttacttggc aaactcttga catttgaatt tctccaataa
      481 ttttaagctt aaaaatggtt cgggcgatct cacaaaaggc gttgctcaac caacccctcc
      541 gattttgatt gcctcgtcag gcattaaccc gaccacagtc tatgtaataa agactgccga
      601 ttataccgat ttcatttgtt atattcaagt agaatttata tgcgtatctt ttactgtttc
      661 aggagaaatg ttaaaggtta cataaagcaa gcggtcattt tctatcagaa atttgcaaaa
      721 atgaatgctc cttcatctct aatttctctt cctcgaacga aaaatagtcc cctttgccga
      781 caataatatg atccacaaag cgaatatcaa ccaattcgca cgccatttcg atctttttag
      841 ttaaacttct gtccgactca ctcggcagac aagaacctga ggggtgatta tgagccacaa
      901 taatcgccgc ggcattatat tttaacgctc ttttaataat ttctcggggg tgaacagcag
      961 tttgattaat ggtgccgaag aacatttttt ctttataaat caatcggttt tggttgtcca
     1021 aaaacagcac cataaaaact tcccgttctt cttcctccaa ctcggtttgg aaatacataa
     1081 tcgccatata aggggcttga atcatctcgc taaattccat ttgttgagcc agatagcgtt
     1141 tggtcatctc tttagaggct tgtaactgaa tgtatttggt tttgcctaaa ccgtaaattt
     1201 tgcaaaaatc atcaatattt gcattcagta attggcgtaa cgagccaaat gccatcaaca
     1261 cactctctgc caatgccagc acaggcacag ctttcgtacc agtccgtaaa aatatcgcta
     1321 ataattcgga atcggttaat gaggctgccc cctgtgttaa taatttctca cgcggcatca
     1381 ttgttggctc ttgagaatct tcatcaaaaa aatcgttcat tttttcctcc atcaatttag
     1441 ttaagcataa aagagccaac ctttgcagac aaagggattt attcaatttt tcgagcaaga
     1501 tcgcaaaaat agtgaagttg tgagcaactc cttattccgc taaaatatgc aaaataaatc
     1561 cacgctttaa ggatagtaaa gatgttacaa aacaaaaaaa ttttagtcgg catttccggt
     1621 gggattgcag cctacaaaac cattgaattt atccgccact taaaaaaggc aaatgcggaa
     1681 gtgcgtgtag tgctaacccc agcagcagaa gcctttgtaa cgcctttgac cttacaagcg
     1741 atttccggca atgcggtttc cacttcacta ctcgacccac aagccgaact ggcgatgggg
     1801 catattgagc tggcaaaatg ggcggattta atcgttatcg ccccggcaag tgccgatttt
     1861 attgctcgcc tgcgtatggg aatgggcaac gatttacttt ccactctctg ccttgcgact
     1921 gcaagcccga ttttgcttgc accggcaatg aatcaacaaa tgtacaaaca gattgcggtg
     1981 caagaaaatc tgtctgtttt agtcaatcgt ggcgttcagc tcatcggccc gaatagtggc
     2041 ttccaagcct gtggtgatgt gggggctggc agaatgtctg agcctgaaga aatttaccaa
     2101 gcggtgttgg ctcatttctc caacaatcaa gatttagagg gcttaaatgt aaccattacc
     2161 gccggaccga ctcgtgaggc gattgaccct gtgcgttata tcagcaacca cagctccggc
     2221 aaaatgggct acgcgattgc taatgccttt gccgaacgtg gagcaaaggt tacgttgatt
     2281 agcggtccgg tgaatttacc aacgccgaaa aatgtggaac gaattaatgt ggaatctgcg
     2341 gttgagatgg agcaacaagc ggtcaaattt gcccaaaaat ctgcgatttt tattggctgt
     2401 gcagcggttg ccgattaccg aatggcggca gtctctgacc aaaaaattaa gaaaaccgat
     2461 gccaatgatg aactcacgct aaaattggta aaaaaccccg atattatcgc taacgtcgcc
     2521 aatcttgccg aaaaccgccc ttttgtggtt ggttttgccg cagaaacgca aaatgtggcg
     2581 gagtacgcta aatcgaaact cgaacgcaaa aatctggata tgatttgtgc caatgatgtc
     2641 tcaggcggac aagtgttcgg gcaggatcaa aatgcgttgc atttgttctg gaaaaatggt
     2701 gaaaaaacgc ttcctctggc ggataaaagc aaattggcag aaagtttggt acaggaaatt
     2761 gtaaaacatt atcgagaaaa ataatgaaat tactggttag ccattttcat caaggcgaag
     2821 ggaaatttcc tacattctca gctggtactg ccgttcgatt agggagtgaa tgtgtaaact
     2881 acccaaactg gtttgcctgt gaaattgacg gttggaaaac ctttgtgcca agccatttta
     2941 ttagcgaaga taaattgata tgcgattata acccaacgga acttgttgca gcacaaggcg
     3001 agagagtaga attccttgaa ctacattacc aatgggcaat tgtcaaaaga gaaaacgaaa
     3061 ttggctggct accttgtgaa atcttaaggg taataggatg aaaattatgc aacttacgtc
     3121 aaatcagtat atcaacgaca ttaaacatat tcttttatct gctcgccaaa aggcgtatca
     3181 agccgtgaat tcggcaatgg tcgaagccta ttggaaaatc ggtgaacgaa ttgtgcaaga
     3241 agagaaaaat atggtcaaag atcaaaatga aaaagtataa tattgtattt tcagcagata
     3301 aaaactacct gccatattta gagattgcct taaaatcact attagctcat cataatcatt
     3361 tatctatttt tatattgagt actggcgata tttctcacca ctggatagaa acattacgcc
     3421 cctatttaga aaatagatat tccacattaa gacttggcta tttagattca aataatttag
     3481 caaattttaa ctccaac
//
LOCUS       DANLUA010000206         3491 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLUA010000206 DANLUA010000000
VERSION     DANLUA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            58..846
                     /locus_tag="PXB44_002350"
     CDS             58..846
                     /locus_tag="PXB44_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282779.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRISNEIKAKIINKINDFEG
                     FKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMVYKF"
     gene            complement(883..3321)
                     /locus_tag="PXB44_002351"
     CDS             complement(883..3321)
                     /locus_tag="PXB44_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
                     /protein_id="HDL5282780.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     KFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVVNNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTLGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNCSEPTKLRNATTKTT
                     KENRSFTFLILMVF"
BASE COUNT         1050 a          644 c          678 g         1119 t
ORIGIN      
        1 tgtgagatag agaaagtatt taaacaagct cctttaccct tttcagggca aaaacggatg
       61 tttttatccc atttcaaaca ggtgttaaat gcaaatattg aagcagacgg gaaagattgg
      121 actattatag atgtttttgg tgggagtggg ttgcttgcac ataccgcaaa gcgtgagaaa
      181 ccgttagcaa gagtgattta taatgatttt gataattacg ccgaacggct aaaccatatc
      241 aaagagacaa atcaacttcg ccaagaaatc taccaaattg ttgatgaaat tataccaaaa
      301 aataagagaa ttagcaatga aattaaagct aaaatcatca ataaaatcaa tgattttgag
      361 gggtttaaag acctcaaatg cttaagttct tggttattgt ttagcggtga gcaagttgca
      421 actttagatg agttatttaa gcacgatttt tggcactgtg tgcggcaaag tgactatcct
      481 gaagcaacgg gttatcttga tgatattgag gtggttagtg agtcctttca tcaattattg
      541 ccgagatttc acgacaaaga gaaagtatta ttgattcttg acccacctta tctttgcaca
      601 agacaggaaa gctataagca ggaaaattac tttgatttaa ttgatttctt acggttgata
      661 gaccttacta aaccacctta tatcttcttt agctcaacca aaagcgagtt tattcgcttt
      721 attcactata tggttcaaaa cagaaaagaa aactggcaag cgtttgaggg agcggaaaga
      781 atagtggtta atgcctcggc aagttataac ggcaaatatg aggataatat ggtgtataag
      841 ttctaaaaat ttaaacgccc tttgatagtg atttaaaggg cgttaaaaaa ccattaaaat
      901 taagaaagta aagctcctgt tttctttcgt agttttcgtg gttgcgtttc gcagttttgt
      961 tggctcgcta caattactgt tcattgtaat gtttaatcga ccattggttt tgctaggatc
     1021 agtttcacca aaataaccgt taaaaccttg gacggtgcct tctgctgctt tgattttttc
     1081 agcaagtatt tgttgctttg cagcactttc atctgttacc ttgagtactt caaagtattc
     1141 gttttcacga atgagaccag tagcaaagtc taaatcgatg aaaactttac gcgcattaaa
     1201 aatagctgtg atctctgcaa cgcttaaagg ttttttcaaa cggataaaat tagttggcaa
     1261 gtgtggaaaa cgttgatatg tgaaaatatc tttgtccaaa tgacgagaaa cctgatctgg
     1321 actagtaatt gcttgaactt tcacttggtc aattgttgga ctaactatgc cggttagtgt
     1381 aatgtttgct gtttgggctg tattatgatt gacaaccatc gctccttcat cgctattctg
     1441 aatacggcgg aacgtgagat cattgccgtt gacatctaag cgcccaccac gataaccaaa
     1501 atagatattg ttaggatcca tttgatctga tgaattgagg attacggtag gtctgccact
     1561 cacaatacct agctctgaaa atgcagattt tttgttattt tcatccgctt gttgatttaa
     1621 tataacagtg ccgtcaccaa cgctaacgga gcctttgttt tctcctttgc cgttgatagt
     1681 tagtgtccct tgtccgattt ttgataatct gtcaccagca gggttactga ttttccagat
     1741 cacattttta tctttatcaa tatcaacacc ggctcctaac caagtaatgc ctgctttcat
     1801 accttccaca gtggtattac catgaaaatg gattgctcct gcaccttggt tgatattctc
     1861 tgtgagactt agcgtattgt tttcacccaa aatatggaat gttttaccgt gttggagttg
     1921 aggattccaa gggttatcgc ctgtattacc attatgacct tgaagtgttg gtaatgaagt
     1981 atctgctaaa ttaactgtaa gaggagcatt ccctcctacg attgagctgg tattgccaga
     2041 aggtgtccaa gtgagtctag caccatttgc attaatagcg cctgcattat tttctttttc
     2101 taaagcacgt acataatgtg ggtggtgata gttccagtaa gatttataaa catctccccc
     2161 aagcgtatta ttctctgaga aatagtcact atatacacct aacacaaccc attttttggt
     2221 acgaacatca taaccaaaaa gagcggagcc actatcgcca ggtaaaccga gagttgttaa
     2281 gggaccgtag ccgtggtctt taaatacatc gtttatggta ttattttcag tttttgctac
     2341 taaaatacgg ctgttaaaat cgctgcctgg aacgcttgga tcgccattag taacaggcag
     2401 aacgctacct cctgtcaaaa aaggaccgtt accgacttca atacgcccgt tgtttaagcg
     2461 atctctttcg ttgttaacca ctgcttgtct accagaaccg gcacgaataa acatggggaa
     2521 aataaaaggg tcgctaaata cactataaaa atcttgtaca ttttcggtag tgcgttcaat
     2581 ctcattagct ggagcgactt cagttactaa tttcgataac ctaggtgcgt ggtagtccca
     2641 gcggggcgtt gggttacctg aaccatctaa aatcagtttt tctccttcgt cattacgttc
     2701 acgtttttct atgcctttcc cgagatctgt tctttcttta tttggatcag gcatatagtt
     2761 attacggcgc accacactat aattgtaatg atggtcgtcg gctttttcgc tagatgaatc
     2821 accaaattga atgtcccgat agccttggtt gtggtttaca ctaataacgt attgtggatt
     2881 aactaatgtt gcaatccctg tttgacgagt aatggaagag aagtcaatca ttggaataga
     2941 catagtctca cctgttactg tttcaaaaac aggtagatca ttagagtctt catcacctcc
     3001 accatagaga aattcataac aaccgggtaa actttcatta tcaaagcatt cagataggct
     3061 tacattcggc ttaggtgaaa tatttgattc cacttcaacc tgcttagttg gtgtaacctg
     3121 taaaaaagta ccgatgtttt tgcccgtttt atcaataact ggaatattgg ttgccccaac
     3181 aaaaaatttg cctttatttt ctgctaaatc acgaaaatat tgataatcga catcaccacg
     3241 tacaatagat gccagtaatg ttggtgaaag agaaatagca attgatgttg ctagaagaga
     3301 gagcttgaaa gtatgcttca ttattatttg aaacctgtat aaatatgtaa gtagatcaga
     3361 tttgagcgct attaaaaaga ttatttaaat cgattaaata ttcatttaaa taccacacaa
     3421 ttattcagcg cgattatctt gttcggcata aatctgctca atacccaaat aaggtaataa
     3481 ttaaaataat a
//
LOCUS       DANLUA010000207         3486 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLUA010000207 DANLUA010000000
VERSION     DANLUA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            48..554
                     /locus_tag="PXB44_002352"
     CDS             48..554
                     /locus_tag="PXB44_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL5282781.1"
                     /translation="MAMSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGV
                     NFFELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQE
                     CLRGTLNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSD
                     YAQGCSCN"
     gene            613..1326
                     /locus_tag="PXB44_002353"
     CDS             613..1326
                     /locus_tag="PXB44_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL5282782.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1437..2441)
                     /locus_tag="PXB44_002354"
     CDS             complement(1437..2441)
                     /locus_tag="PXB44_002354"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL5282783.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(2529..3368)
                     /locus_tag="PXB44_002355"
     CDS             complement(2529..3368)
                     /locus_tag="PXB44_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL5282784.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
BASE COUNT         1034 a          715 c          675 g         1062 t
ORIGIN      
        1 agtatctaat ttaatttttt aagattaaca attaagagga gcatattatg gcaatgtcaa
       61 atatcggttt agataaagaa atcgcaaaaa aattagcaga tgagttaaat ggtttattag
      121 ctacttacca agtgttttac actaatgtac gtggctacca ttggaatgta cgtggtgtga
      181 acttctttga attacatgca aaatttgaag aaatctacga tgatttagtg acaaaagtag
      241 atgaaattgc agaacgtatt ttaaccttag gtcacacacc tcaaaatgcg ttcagccaat
      301 atttaaccaa gtcacaaatt caagaacata ttggtgtttc agctgcacaa gaatgtttac
      361 gtggtacttt aaatggtttc aaaacattac tgggacaaca acgtgaaatt cttgctctag
      421 caaatgatgc aaacgatgaa ggtactgctt ctcaaatgag tgattacatc aaagaacaag
      481 aaaaattagt atggatgttc actgcagctt gtgctgaatg ttcagactac gcacaaggtt
      541 gttcttgtaa ctaagttgca ataatataat taacccccga taatgaaaga ttatcggggg
      601 ttactttttc agatagctat gtcgcaagga tttcaattta aacagttttt tattcggcac
      661 gacagatgtg caatgaaagt caatacagat ggtattctat tgggagcgat tgctcacata
      721 aaagattgtc agaatatttt agatctgggt ataggttctg gtttagtagc acttatgctt
      781 gctcagagaa cagatgattg ttgtcacatt actgcgttgg aacttgaacc aaatgcctac
      841 caacaagcgg tcgaaaatgc ccaaaattct gcatgggcaa atcggatttc cgttttgcaa
      901 ggtgatgtga tgcagcaggc atttgaacag aaatttgact tgattgttag taacccacct
      961 tattttgcag attcacttgc agctcgaacc acagagagag atttagctcg ctcaattagc
     1021 cattacagcc acttgaattg gctggcaaaa gcaaaaccat ggttagcaga gaacgggaaa
     1081 attacactga ttttacctat agcagctgca gaaaaattag ttgaccaaag tgaacaaatt
     1141 ggattgtatt gtgttgaaca atggttaatt tttaccaaga gcggcaaatt ggctaaacgc
     1201 agtatagtga gttttagttt aaacaagcaa aagcgtgaac ataaagagct gatcatctat
     1261 gatctagata attgttacac tactgagttt aaaagactta cccaagattt ttatttgaat
     1321 ttttaattta ctccccgggt tagtgttttc cacaaatctt cttcagctac gatgatctag
     1381 ccttaagcta aaaagcagat taccacccaa gataatccgc tttttagact tagctattat
     1441 gccaaacttg ctaccattac cgctttaata gtgtgcattc gattttctgc ttgctcgaat
     1501 gccacattca ttggcgattc aaacacctct tcggttactt caatgccgtt tgccagtgcc
     1561 ggatattttt cggcgatctc tttaccgact ttagtttcac tgttgtggaa cgccggtaag
     1621 cagtgcataa atttcacttt cgggttgtcg gttgcttgca ttaattcagg cgttactcgg
     1681 taaggcatta ataaatctat acgttcagtc cagctctcta gcggttcacc cattgaaacc
     1741 caaacatcag tatgcacaaa atcgacacct ttaattgcag tctcaatatc ttcggttacc
     1801 gtcattcttg cccccgattc agctgcaaat ttttcacaca ttttcacgaa tgcatcttcc
     1861 ggtaataagg ctttcggggc acaaattcgc acgtccatcc ccaatttcgc cccaatcaac
     1921 aggagtgaat tacccatatt attgcgagca tcgccgatat agacataact gatttggcta
     1981 agcggttttt cgcaattttc gatcatcgtt agcacgtcag ccaacatttg ggttgggtgg
     2041 aattcatcgg ttaagccgtt aaacaccggc acgccggcat aatcggcaag ctcttgtact
     2101 accgattgtt taaagccgcg atattgaatc gcatcataca ttctgcctag cactcgggcg
     2161 gtatctttca ttgattcttt atgcccgatt tgcgaggagt tcgggtcaat ataggtaacg
     2221 tgagcccctt gatcgtaagc tgctacttca aaggcacaac gagtacgggt cgaggttttc
     2281 tcaaaaatta aggcaatatt tttgcccttt aatttttgct gctcagtgcc ggcatatttc
     2341 gcccgtttta aatctcttgc taaatccaac aaaaagcgaa tttcacgctc agtgtggttt
     2401 actaaactga gtaaatgtct gttttttaag ttaaatgcca ttttgtttct ccctgtaaac
     2461 attaaaattt attttatgca aaataattgc ataaaaattc acttatagca aacagaattt
     2521 gcttaccctc attcgggtaa ataaaactcg cctttgccga ctaaaataac ctctccgcca
     2581 acaaaaatct tcccgttttg atcgactttt aataacaggc gattcggccg ttgaatttca
     2641 tcaccttgtg agatctgaag ctggattggt attttgcctt gtgcaatgtg ccaaccgcct
     2701 aaatttgccg ctgccgaacc ggtgccgggg tcttcgacaa tcgaaccttg cgattgaaaa
     2761 aacagtcgca ctttcgcctc atttccctca cgacaccaaa catatacact gtctttcaaa
     2821 aatgcttttt cagccagatt tgcatttatt ttgcactttt cgaccgcttg ttgagaagct
     2881 aactcaacca ataactgctc cacgccggtg tttacataac aaggctcgga cgcaatatcc
     2941 gcaacattca gccccaatat tgaggcacat tctgcacggc ttagcggtgt gttttccagg
     3001 ttcacaccat tggttaaagc aaaagtagtc acattatgtt gatgttgaat cgctaccaat
     3061 ccggcattag tttgcaagcg ataggttgtt ggaaaatcaa aggcggaatg caacacaaaa
     3121 gccgctccaa cagttgggtg gccggcgaac ggcatttcat aatcaggcgt aaaaattttg
     3181 agttttttga ccgcttgtga atcggtggaa gattgaataa ataccacttc agaaaggtta
     3241 aattggcgag caataagttg catttgtaag tcggttaatc cgtccgcttc ataaaatact
     3301 gcaagcgggt tgccgccaaa atggcttgtt gcaaacacat tcaccagcaa ataaggatag
     3361 ctctgcattt atcactcctt acataaactt aaagccacat tgggcgaaaa ttctaccatt
     3421 ttggtggcga atcgtaaaat tggaaaattt atcggtatcc ctctttagag ttaactattt
     3481 ttgatt
//
LOCUS       DANLUA010000208         3460 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLUA010000208 DANLUA010000000
VERSION     DANLUA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            152..1474
                     /gene="pepP"
                     /locus_tag="PXB44_002356"
     CDS             152..1474
                     /gene="pepP"
                     /locus_tag="PXB44_002356"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL5282785.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1590..1775
                     /locus_tag="PXB44_002357"
     CDS             1590..1775
                     /locus_tag="PXB44_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL5282786.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            1904..3220
                     /gene="argA"
                     /locus_tag="PXB44_002358"
     CDS             1904..3220
                     /gene="argA"
                     /locus_tag="PXB44_002358"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL5282787.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
BASE COUNT         1099 a          605 c          765 g          991 t
ORIGIN      
        1 tgtaaaggtt atatttaatc ttaatctgtt ttgtttttgc aatcaagatc tcaaatataa
       61 tctttcatct gttttggtta ggctgaattt atgctgcgtt tgtgctaaaa taaggcaggt
      121 ttcgatatag atataataaa aaggagaaat aatggatctc gcttatttgg ctaaaatgcc
      181 acaagaagaa tttaccgcac gccgtgagcg tgtgcttgag caaatgcagg ataattctgc
      241 gttattggtt tttaccgaat cggaaaaacg ccgtaatagt gactctgaat acttgtttag
      301 acctgatagt tacttttggt atttaaccgg tttggcagaa cctaaatcag ccgtattgct
      361 ggtaaaaagc gaaggtaaaa cggaaagtat tatttttgtg cgtaaaaaag accctttaat
      421 ggaaacctgg aatggcagac gtttagggat tgaggaagct ccaaaaacgt tacagcttaa
      481 cgcagctttt gatattgatg acattgaaac cgttcttgca aaaaaattag aaaatctgac
      541 cgcttgttat tgtgcgttag ggatacaaga gtggggcgat aatattttgt ttgcaacatt
      601 tgaaaaaatg aaagtcaatc ggcaaaaagt gccgacaacc ttgattgatt ggcagccaat
      661 gttatcggaa atgcggttga ttaaatcaga atttgaaatt gccttaattc agcaggcgtg
      721 tcatatttct tcaatggcac atattcgagc gatgaaacaa gctcgcccga atcgttatga
      781 gtgggaaatt gagggggaaa ttcaacacga atttagccgt tttggagcaa ggtttccggc
      841 atataattca atcgttgccg gcggtgagaa tgcctgtatt cttcactaca atgaaaatga
      901 tcaggtgctt aaaggcggcg atttattact gattgatgca ggggcggaat ttgcgtatta
      961 cgccggcgat attacccgaa ccttccctat taatgggaaa ttctccgaac cgcaaaaaga
     1021 gctgtacgaa ttaaccttaa ctatgcaaaa agaggcgatt aatcttcttg tgccaaacag
     1081 ctcgattaaa gcggcaaacg ataaggctgt acagattctc acgcagggct tggttcgctt
     1141 aggaatttta aacggtgatg tggaaagcct gattgagcaa aaagcctatc gccaatttta
     1201 tatgcacgga ttagggcatt ggctagggct agatgttcac gatgtggggg attacggcac
     1261 ggagcgggat cgtcctttgc agattggtat ggtgttaact gttgaacccg gtatctatat
     1321 tcctaaagat gcggatgtac cggagcaata taaaggtatt gggattcgga ttgaagataa
     1381 tttgctgatt accgaatacg gcaacaaaaa cttaagcagc ggttgcccta aagaaattgc
     1441 ggatgttgag cagttgatgc aatcgttaaa ttaattcgca aatttttgta aaaaaatgac
     1501 cgcttgcttt gatattttgg ggaatttagc ttaagctaac ccgacacaaa cacagtgttt
     1561 gggcaatcac aacacgaagg agtgtttata tgtattttga aatttacaaa gatgcaaaag
     1621 aagaatttcg ttggcgttta aaagcgggta accacgaagt tattgcaacc aaccaagctt
     1681 atacgactaa gcaaggttgt caaaaaggca ttgaagcagt taaaaaagtt acacctgaaa
     1741 cagaagtaaa agatttaacc aaagctgaag cataatctat aaagccctga tattatttca
     1801 gggctttgtt atttatgcaa atataatgct taattattca atttaaggct tgcggatttt
     1861 tctaaaaaag ataatatgcg tttatattat aaagaggaaa tatatgcaaa acacagaact
     1921 cgcattagct caatggttta ggcaatctac accttatgtt aatatgcaca gaggcaaaac
     1981 ctttgtgatt atgcttgatg gcgatgttat cgaaagcccg aattttgtca atattatcaa
     2041 tgacatcagt ctcctgcaca gcctgaatat caaattagtg attgtatttg gggcaagacc
     2101 gcaaattaac caattgttaa aacaaaatca tattgagccg gagtattacc acaatatccg
     2161 tgtgactaat ccacaatcgc tagaatatgt gaaacaagcg gtagggaaag ttcattatga
     2221 tttctttgcc cgcctttctt tgcgtttacc ccattctgaa gtgattaatg tagtcagtgg
     2281 taatatgatt ttagctcagc cgattggcgt ggtggatggg gtagattata cactaagcgg
     2341 taaaatccgt cgtattaata ttgagagcat taagcagcaa ctggcacaaa attcgattgt
     2401 attacttggt ccgattgccc cgtctgttac cggtgaaatg tttaatctgc cgtttgaaga
     2461 tattgccaca caggttgcaa ttaaacttcg tgccgataaa ttaatcggtt tttgtgaaga
     2521 gcaggggatt ttagatgaaa acggcaagcc aattccagat tatcttccgc aagatggcga
     2581 aaccttgtta cagcagttaa tcgagcgtgg cgaatatcac accccgaaag ctcggttttt
     2641 gcaggcggcg atcacggtat gccgagaggg agtgaaacgc tcgcatttat taagctataa
     2701 agaagatggc tcattgcttc aagagttatt ctctcgtgat ggtattggca cacagttctc
     2761 aatggaaagc tcggaacagg tacgtattgc taattccagt gatattccgg gattattgga
     2821 tttaatccgc ccacttgaac agcagggcat tttagtcaaa cgctctcgag aacaattaga
     2881 aatggaaatc acaaactaca ccattattga gcgtgatggt gtggtgattg cttgtgcggc
     2941 acttaacccg tatccggaag aaaaaatggc ggaaatggcg tgtgtggcgg ttcaccctga
     3001 ttatcgtgat tcctcaagag gggatattct actggaaaat attcaaaaac gggcagtgca
     3061 actgggaatg gagaaactct ttgtgctgac tacaagaacc acacaatggt tccaagagcg
     3121 aggatttagg ctggcaggtg cccaagattt acctcgctat aaacgggaaa attacaatta
     3181 tcaacgtcgt tctaaagtat taattttacc actttggtaa aatattcaat gtttttgacc
     3241 gcttgttacc aagagaacta taaaaggaga actatgaaaa aacatcaacc catcaaaggg
     3301 gctatggcaa ttattactgc tgtagaagat cagacttgat ctgacaattc actctaaaaa
     3361 gtgagagttt cccgtttaga atatgagtgt caaaaatcaa tctaaacaaa aaggaaactc
     3421 tcatgtttta ttctaacaac cctctcatta aacacaagac
//
LOCUS       DANLUA010000209         3393 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLUA010000209 DANLUA010000000
VERSION     DANLUA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..385)
                     /locus_tag="PXB44_002359"
     CDS             complement(<1..385)
                     /locus_tag="PXB44_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL5282788.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEK
                     AAKEKAEAERLAAEKAAKEKAEAERL"
     gene            588..2042
                     /locus_tag="PXB44_002360"
     CDS             588..2042
                     /locus_tag="PXB44_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL5282789.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            complement(2117..3388)
                     /locus_tag="PXB44_002361"
     CDS             complement(2117..3388)
                     /locus_tag="PXB44_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5282790.1"
                     /translation="MAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSIREELQL
                     DGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYFATSAFE
                     IGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGMSAVLLQ
                     GEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVKLGLGNG
                     WELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPALLEEAGM
                     PKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVFAGNLGL
                     AITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPTLAGILL
                     DAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
BASE COUNT          995 a          780 c          710 g          908 t
ORIGIN      
        1 ctaaacgttc tgcttccgct ttctctttcg ccgctttttc cgccgctaaa cgttctgctt
       61 cggctttttc tttcgctgct ttttccgccg ctaaacgttc cgcttcggct ttttctttcg
      121 ctgctttttc cgccgctaaa cgttctgctt ccgctttctc tttagctgct ttttctgctg
      181 ctaaacgttc tgcttctgct ttctctttcg ctgccttgtc ttttgctgct ttttctgctg
      241 ccgctttatc ggtttgttgc tgagtagttt gcggtttagt attcggtttt gccacattgt
      301 ttgagctact gcccgaacca ccactactac acgccgttaa acccaataaa atcaaactgg
      361 ctaaagttac ttttttgata gacataaaat gttccttttg taagataaac aaaaatagat
      421 gtgtataaaa cacacgcata ttgtacaaaa aaaaaacaac tcgtcatcaa gtacttttta
      481 agattttttt gttaaatttc tcctaaatat tctacaatgt cagaggtcta tatcagcttg
      541 aatacaagcg gttatttttt cttaaaattt tacaaaaggg gcttattatg tctgaaatct
      601 caacgctcgc accgcagcta ctttggcagt ggtttgataa agcctgtgct attccgcacc
      661 cgtcttacca cgaaaatgaa ctggctgaat ttattgtgaa ttgggcaaaa tcgaaaaatt
      721 tctttgttga acgtgatgaa gtgggtaatg tgttaatccg taaaccggca actaaaggta
      781 tggaaaaccg ccaaactgtc gctctgcaag cccatcttga tatggtgcca caagccaata
      841 ccggcacggt tcacgatttc actaaagatc cgattcagcc ttatattgac ggcgaatggg
      901 taaaagccaa aggcacaaca ttgggggcag ataacggcat tggattggct tcgtgcttgg
      961 cggtgttgga tagcgatgat ttagcccacc caaccattga agtgttgctc acaatgaccg
     1021 aagaagccgg tatggaaggg gcgattggtt tacgcccgaa ctggctaacc gccgatatta
     1081 tgattaacac cgacaccgaa gaaaacgggg aaatttacat cggctgtgca ggtggggaaa
     1141 atgtgaatgt gacactgcca attttcttgg tgcctcatca gcaagatagt gcagtaaccg
     1201 ttacgcttaa aggcctaaac ggcgggcatt cgggctgtga tattcacacc acccgagcga
     1261 atgcgattaa agtgttcgcc cgcattttgg aacaagccca caacgaagtg gaattccaaa
     1321 ttgcagaaat tcaaggcggt tcggtgcgga atgccattcc acgagaagcc cacgcaacct
     1381 tggttttctc tgcaaaaaat cagcaaaaat taaccgcttg tttaagccaa accgccgaac
     1441 aagtaaaagc agaactcaaa attgccgagc caaacttcca atttttaatt gaagaagctc
     1501 aactgcctga gcaagccttt gaccttgcca gcactgctcg agtaatccat ttcttaaatg
     1561 tgatgccaaa tggtgtggtg cgtaatagcg atgtggtgga aaatgtggtg gaaacctcac
     1621 tcagtattgg tgtgcttaac accgaagcga gtgaggtgaa agcgaccatt cttgcccgct
     1681 cattaattga gggcggaaaa gaggaaattc gcagtaaaat ccgctcatta accgcattag
     1741 tgggagcaga agtggaattt tcgggcaact acccgggctg ggagccaaac ccaaattcca
     1801 aaatcacgcc attaaccaaa gcgatttatg atgaaatttt aggttatgaa tctcaaatta
     1861 aagtgattca cgcagggcta gaatgtggct taatcaacaa aatctacccg aatatggatt
     1921 ttgtctcgat tggaccaact attctcaacg cccattctcc ggacgaaaaa gtgcatattc
     1981 cggcagtgga gatttattgg aaactcctaa ccaaactact tgaacaagca cctgagaaat
     2041 aaatttaatc aaaaaaatta tataatcttg ccccgtaatt aattgaataa aacctcaatt
     2101 aattacgggg tagctcttat tttctctgtt ttaaaaaatg taccgtgaaa gcagcaatta
     2161 ataagacgac tgctgcacct aaataaagct cgttcttttt ccagccggcg tctaataaaa
     2221 tacctgcaag agttggggaa acaattgagc ctaaacgccc tacaccgatg gcggtgccta
     2281 cgcccgtgct gcggaaatcc ggtgcgtata gggttgggtt aatggtataa agcccggtaa
     2341 tgcagccgtt aattaatccg ccaaccaaaa tggctagtgt tatcgcaagc cctaaattgc
     2401 cggcaaacac aaaggcaacc acagcagatg cagccagaat actgaatgcc tgcaaaatat
     2461 gttttgccgc ccagcgactg actaaaaagc cgaaaattaa cgagccgatt gtaccaccaa
     2521 ctgaaattgc cattcccaca gtttggcttt gggttttagg cattccggct tcttctaata
     2581 gtgccggagt ccaagagctg ataaagtaaa atgaggctga aattgcaata aaggcaagcc
     2641 aaattagcaa cgtattgcgg cgatattcag gctcgaataa acgggtaatt gccactttgc
     2701 ttttactttt tgcctcaatt tcaggaagtt cccagccgtt tcctaagcct aatttaacgg
     2761 caatttgatt taaacgagct ttagcattca ccgggtgttt tgaaattaaa aattccaccg
     2821 attcgggcaa tacaaagaat aataaaacga ataccgcacc ggttaaaatc gacccaacga
     2881 ggaaaaccgc acgccagcca tattctcctt gcaataacac cgcagacatt ccgcccagca
     2941 ttgcaccaat cccaaagcct gaggcataaa ttgcaatcgc taagccacgc catttttggc
     3001 tggagtattc actcactagc acattagtgc cggttaaaat ggcggctacg cctaaaccgg
     3061 ttaaaacacg ccatacacca atttcaaacg cagaagtagc gaaataagtt aggagcattc
     3121 ctaacgtggc taagcctgtg ccgattaaaa gcaacgggcg gcgaccaaat ttatcagcaa
     3181 gtggtgaaag aaagagtgag cctatcgcca taccgaataa accggcactc attaagctgc
     3241 cgatggctgc accgtctaac tgcaattctt ctcggatgct tttggcggta aaggcgagtg
     3301 ccattaaatc aaagccgtcc agcatattaa gtaacaccgt aagcccaacc actaaccatt
     3361 ggaatgaagt cattttgtta tgagcaattt ttt
//
LOCUS       DANLUA010000210         3389 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLUA010000210 DANLUA010000000
VERSION     DANLUA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            410..928
                     /locus_tag="PXB44_002362"
     CDS             410..928
                     /locus_tag="PXB44_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5282791.1"
                     /translation="MRYIKYSLFLVSTMLISGCAELSAINDKVGDWAGQMNQTLYGSK
                     DGSVEQHNGRQVYTKTRNGKYVYQDSGTLQLKRNIDDVFLKVRREFGFRPTNADSDSR
                     KVYHTVQGTFYHVSGYFESDVDTDGVHLGENYLEVIIEKGSKNQVNVEWKATGSARWL
                     DSAKQRLLNTLK"
     gene            1088..3139
                     /locus_tag="PXB44_002363"
     CDS             1088..3139
                     /locus_tag="PXB44_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5282792.1"
                     /translation="MKLFICEKPSQARDLAGVLGATSKGEGLLATADKSIIVTWGFGH
                     LVEQYQPEDYDEAWKRWAFETLPIIPAQWKMTPKKESKKQYSVVIGLIKKATSVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLTLVVNRDREIANFVPKSH
                     YALAVQLTTTSGETFVAGLAVPEQYLDESGLCLDSRVIQQAEAQIKQTGLAKVVTVET
                     KREKKSPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QFSDVPNVIRSLVASDSGLQTLLPKLNLRQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLEMDKTVATLQSSGHTLVARGNVIVAQGWRILFGKN
                     VDEETGNDDEKQGLPVLTKNQQCNVTHSEVRTLQTKPPAHYTEGTLLDAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLLKKKKSLVASAEAVALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDAFMQKQEAFVKQLIGSCMQQGMSLGNIEIRKCSEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSASNKSAKKSSNLNVLDQLNKLRSQ
                     L"
     gene            complement(3200..3337)
                     /locus_tag="PXB44_002364"
     CDS             complement(3200..3337)
                     /locus_tag="PXB44_002364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282793.1"
                     /translation="MTLQSRFRQLQQTNVAGIIHLMFSWHSHKVEFPLRMLGDILAGV
                     D"
BASE COUNT         1066 a          659 c          721 g          943 t
ORIGIN      
        1 aaaaataact ttgatagagg ataaatatat gaatttccct ccaactcgat ggaaaatatt
       61 acaccagtat caaactgagc tagagctctg ggttgaacaa gccacagaag agcatatcaa
      121 gcaatacttg actgaaattc acggatgtgt atacccagat gactgggaaa atgtgcgtat
      181 atcccatatg tcatctcaaa ctcatatttt ccatagctgt gaacgttgcc tagaacttat
      241 ctctgcgata ttaacaatct cacaactaag cagacccgat ctaaatggtc tactgtgtga
      301 cagaaagcta taatattttt tctctttgta agtaaaagag ttttaagtag attttaaccg
      361 cacttttgca aaaaatgaac gcagataacc cgcttgtaag gagaattcta tgcgttacat
      421 caaatatagt ttatttcttg tttctacaat gctaatttca ggctgtgctg aactctcagc
      481 aattaacgat aaggttgggg attgggccgg gcaaatgaat cagacgctct atggctctaa
      541 agacggctct gttgaacagc ataatgggcg acaagtttat actaaaaccc gaaatggtaa
      601 gtacgtttat caagattccg gcacattgca gttgaagcgg aatattgatg atgtattcct
      661 gaaagttcgt cgggaatttg gcttccgccc aacgaatgct gatagtgact cccgtaaagt
      721 ttatcatacg gtacaaggta ctttttacca tgtttccggc tattttgaat ctgatgtaga
      781 tactgatggg gtacacttag gtgaaaacta cttggaagtt atcattgaaa aaggcagtaa
      841 aaatcaggtg aatgttgaat ggaaagcaac tggtagtgct agatggctcg atagtgcaaa
      901 acagcgttta cttaatacat taaaataata actcatattg ctcctagaac ctcttcccag
      961 tggaaacact gggatttttt atgtccttat ttctcaaatt attacatcct acgagacaat
     1021 aagtcgttga atgctattta atcagcacat tactatttgc ctatctgtat tcattttagg
     1081 aaataatatg aaattattta tttgtgaaaa gccatctcaa gctcgagatc ttgccggcgt
     1141 attgggtgct acttcaaaag gtgaaggctt gctggcgact gcggataaat ctattattgt
     1201 cacttggggg ttcgggcatc tcgttgagca atatcagccg gaagattatg atgaagcgtg
     1261 gaaacgctgg gcatttgaaa cattacccat tattcctgct cagtggaaaa tgacaccgaa
     1321 aaaagagtct aagaagcaat attctgttgt aatagggctt atcaaaaaag caacctccgt
     1381 ggtcattgcg accgatgctg atcgagaagg tgagatgatt gctcgtgagc tgttagattt
     1441 agctggatac cgaggtcaaa tccaacgttg ctggctatca gctctggatg atgccagtat
     1501 tcgaaaggct cttcaaacac tcaaatcagg taaagaaacc gaggctcttt actacgccgg
     1561 tatgggacgt agccgctcgg actggttgat tggaatgaac ttctcccgtt tatttacctt
     1621 aatggctcaa gcaaaaggct attccggtaa gccattgagc gttggtcgag tacaaagtcc
     1681 aacattaact cttgtggtta atcgagatag agaaattgct aatttcgtgc ctaaatctca
     1741 ttatgcgtta gcggttcagc tgacaacaac ctctggtgaa actttcgttg ccgggcttgc
     1801 agtacctgaa cagtatttgg atgagtccgg actttgcctt gatagtcgtg tcatacaaca
     1861 agccgaagct caaattaaac aaacaggctt agccaaagtg gtgacggttg aaactaaacg
     1921 agagaaaaaa tctccaccgt tactctacgc attaagtgat ttacaaggcg aatgtaaccg
     1981 cttgtttggc tttggggctc agcaggtgtt ggatatcgca caatcccttt acgaagagca
     2041 taaaatcacg acttatcccc gtacagactg tggttatctg ccagaatcac aattttctga
     2101 tgttcctaac gtaatccgct cattagttgc ctctgattca gggctacaaa ctctcttgcc
     2161 taaattaaat ttgcgacaaa aaagtcgggc gtgggacgat aaaaaaatta ccgctcacca
     2221 cggtattatc ccaaccatta aaaaagctga tttaagtaaa ttgagtgagg aggaaatgaa
     2281 ggtctatgat ctcattcgcc gccgatatct tgctcaattc ttaccgcact tggaaatgga
     2341 taaaaccgtt gccactctgc aatctagtgg gcacactctg gttgctcggg gaaatgtcat
     2401 cgttgctcaa ggctggcgta tcttgtttgg caaaaatgtg gatgaggaaa ctggtaatga
     2461 tgatgagaaa caaggattac ctgtattgac taaaaatcag caatgcaatg tcactcattc
     2521 tgaagtccga acattgcaaa ctaaacctcc cgcacactat acagagggga cattactcga
     2581 tgcaatgaaa aatgccgctc gctttgtgac tgacccaaga ctcaaacaac gcttacgtga
     2641 aacggaaggt ttaggcacag aagcaacccg tgccggatta attcaaggcc taattgacaa
     2701 aggatttctc cttaagaaga aaaaatcact ggttgcctca gcagaagccg ttgccttaat
     2761 tgatagtttg ccggacttac tgaaaaatcc cggtttaacg gcattatggg aacaagccct
     2821 caatcagatt gccgaaggta gtatgacact tgatgcattt atgcaaaagc aagaagcatt
     2881 tgtaaaacaa cttatcggca gttgtatgca acagggaatg tcacttggga atattgaaat
     2941 ccgaaaatgt tcagaatgtg gtaagccaat gagaaaaatc aatcacgcta agggaacatt
     3001 ctggggatgt actggttatc cagattgcca acataaagag gctgataaaa aggtaaaatc
     3061 agcttcaaat aaatctgcta agaaaagtag taatttaaat gtactcgatc aactgaataa
     3121 attaagaagc cagttataaa ttcaagtaga ttatggtatt atcaaggaac aaagaacccg
     3181 ttttgtagat tgtcggttat caatctacac cagccaaaat gtctcccagc attctgagag
     3241 ggaactccac tttgtgggaa tgccaactaa acattaaatg gataatgcca gccacattag
     3301 tctgttgtaa ttgtctaaat cgtgattgca acgtcataat attacccgat tggggggatt
     3361 atgacgttgc aattactgct aactattct
//
LOCUS       DANLUA010000211         3389 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLUA010000211 DANLUA010000000
VERSION     DANLUA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1438)
                     /locus_tag="PXB44_002365"
     CDS             complement(<1..1438)
                     /locus_tag="PXB44_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5282794.1"
                     /translation="MTLQIHSIRTKVKTPLAKRIAHALTDRKILQFRFDAHLRNQVWQ
                     RLTKTQKALLNRINAAGIDALPKRELDKLLKELKAEIAKNYQETTAYIQPELSGFFAV
                     EKQALTELYNDEIGYDFFNQVPSYQRKATQQATIIAGMPLESWFEKQGSELSFKFEST
                     IRQGLLEGRQTPEIAKATAELFGISRRHADTLVITAVAKVADDAHKALRDENLDLIKG
                     EMHLSTLDTRTSTVCQVRDGKMWDTDYKPIGHSLPYQRPPLHPRCRSILQLVMKDWKE
                     LGFDDVKEMPKSTRASADGQVSEKTNYEEWLSSKTPEEQDKALGKGKADLWRRGVITF
                     SDMLDQSARPLTLKELYSLKNSSSVDKMDSVELVRKKAVAVEPMITRDVVSIITASGG
                     EPAGLDFRLKSLSSLQRKIDTEIMAGVSKEQAIASIRDVIRYTAILDEQRFVEQYQKM
                     QKDLEKQGYSTIIVKNTWKSNNAYKGINT"
     gene            complement(1386..2789)
                     /locus_tag="PXB44_002366"
     CDS             complement(1386..2789)
                     /locus_tag="PXB44_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDL5282795.1"
                     /translation="MPNSISADFAALKAKTKIIDDLLGGTQKMRQAGQTYLYKMKMEE
                     EDGYKNRLNRSTLYPALRETLSQMLGRVFFNPINVEDVHKQIEPLFDDIDLEGNNLDV
                     FASRWFYAALAYGVSYCLVDFVRVENARSLAEEKAQNARPYLVHIKPQQVLGFKTAKI
                     NGKTAFTQFRYKETVTEDDGEFASKQVNYIYVYEIGRVRKYKNDEGGLSLVADMQIKA
                     QNTPLAFVPIVPFITKPIDVWGMGEPPLMELAYLNIKHWQSQSDQDNILNIARVPLLA
                     IFSDTQIKSLEIGSSAISLPKDSTMQFIEHSGSSINAGLTSLKELEDQMKTAGAKLLT
                     KTALAMTDSQAKDEAGKEISQLRHYANKFEDALDLAFEYIGHWLGIGVDEVGTSQISG
                     NIDNDLDPNASMATIIQLRNAGVISNHSTFEEAKRRGLLSDSAKWEDEQARLQAEGLS
                     DDFTNPFNSNQSENAAS"
     gene            complement(2802..>3389)
                     /locus_tag="PXB44_002367"
     CDS             complement(2802..>3389)
                     /locus_tag="PXB44_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL5282796.1"
                     /translation="GDYLESSDAQIFRDKYQELEFKPNPDFNGPYYGLDFGFANDPTA
                     CVKCWVFDDDLYIEREAGKVRLELDETAAYLANALPDLSNYTVRADSARPESISYLKR
                     HGLPYIQPVPKWKGSVEDGVEHIKSYRKVYIHPSCTEVLKEFRLYSYKTDRLSGDVLP
                     IIIDAHNHYIDAIRYALNPLIQARGEAKQCALKVY"
BASE COUNT          914 a          768 c          619 g         1088 t
ORIGIN      
        1 acgtattaat gcccttataa gcattatttg atttccaagt gtttttaaca atgatcgtag
       61 aataaccttg tttctccaag tctttttgca ttttttggta ttgctcaaca aaacgctgtt
      121 catctaagat tgcagtgtag cgaataacat ctcgaatact tgctatcgct tgctcttttg
      181 acactccggc cattatttcc gtatcaattt ttcgctgcaa agatgatagt gatttcaagc
      241 gaaaatctaa gccggcaggc tcgcccccag atgctgtaat aattgatact acatcacgag
      301 taatcatagg ctcaactgca acagcctttt tacggacaag ttccacacta tccattttat
      361 ctaccgaaga actatttttt aatgaatata gctcttttag tgtcaatggt ctagcacttt
      421 gatctaacat atcgctaaac gttatcactc cccgccgcca caaatccgcc ttgcctttgc
      481 ctagcgcttt atcttgctct tccggtgttt tgctactgag ccactcttca tagtttgtct
      541 tctccgacac ttgcccgtct gcactcgccc tagtgctttt cggcatttct ttcacatcat
      601 caaagcctaa ctctttccag tctttcatca ccagttgcag aatcgaacga cagcgagggt
      661 gaagtggtgg gcgttgatac ggcaaactgt gtccgatagg cttgtaatca gtgtcccaca
      721 tcttaccgtc tcgcacttga caaacagtac tggtgcgggt atcgagcgtt gataagtgca
      781 tctcaccctt aattaaatca agattttcat ctcgcaatgc cttgtgagca tcgtccgcca
      841 cttttgccac agcagtaatg acaagtgtat cagcgtgccg tctggatata ccaaataatt
      901 cagccgtagc tttggcaatc tcaggcgttt gcctaccctc taacaagcct tgccgaatgg
      961 tgctttcaaa tttaaacgaa agctctgatc cttgcttttc aaaccaactt tcaagcggca
     1021 ttccggcaat aatagtcgct tgttgcgtag ctttacgttg atatgacggc acttgattga
     1081 aaaaatcata gccgatttca tcattgtaaa gctcagtcag tgcctgtttt tcaaccgcaa
     1141 aaaaaccgct taattcaggc tgaatgtaag cggtcgtttc ttggtaattt tttgcaattt
     1201 ctgctttgag ttctttgagc agtttatcca actctctttt cggtaaggca tctatcccgg
     1261 ctgcattaat tcggtttaat aatgcctttt gcgtttttgt gagtcgttgc caaacttgat
     1321 ttcgtaaatg agcatcaaaa cggaactgca atattttacg atctgttaat gcgtgagcga
     1381 tccgtttagc tagcggcgtt ttcactttgg ttcgaattga atggatttgt aaagtcatcg
     1441 cttaaacctt ccgcttgtaa ccttgcttgt tcgtcttccc attttgcact atctgacaat
     1501 aaacctcgtc ttttagcttc ttcaaacgta gagtgatttg aaatgacacc ggcattgcgt
     1561 agctgaataa tggttgccat tgacgcattt ggatctaaat cattgtcgat attgcctgaa
     1621 atttgcgaag tcccaacttc atcaacgccg atacctagcc aatgcccaat gtactcaaaa
     1681 gccaaatcga gagcatcttc aaatttgtta gcataatgtc taagctgtga gatctcttta
     1741 cctgcttcat ctttagcctg actatccgtc attgctaatg ctgttttagt tagcagtttc
     1801 gcaccggcag ttttcatttg gtcttctagt tctttcagac tggttaaacc ggcattaata
     1861 gacgaacctg aatgctcaat aaactgcatt gtactgtctt tcggcagaga tattgcactt
     1921 gagccgattt caagggattt tatttgagta tcagagaaaa tagctaatag cggtacacga
     1981 gcaatattca aaatattgtc ttgatctgac tgactttgcc agtgcttaat atttaaataa
     2041 gccagctcca ttaaaggcgg ctcacccatt ccccaaacat caatcggttt agtgataaat
     2101 ggaacaatag gcacaaatgc aagcggtgta ttttgagctt tgatttgcat atcagcaacc
     2161 agtgataagc caccctcatc gttcttatat tttcggacac gtccgatttc ataaacataa
     2221 atgtagttca cctgtttaga agcaaactcg ccatcatctt ctgtaactgt ctctttataa
     2281 cgaaattggg taaaagcagt tttaccgttg attttggctg ttttaaagcc cagcacttgt
     2341 tgcggcttaa tatgcaccaa atacggacgt gcattttgag ctttttcttc tgcaagactg
     2401 cgggcattct ctaccctcac aaaatccacc aagcaataag atacgccata agccaatgca
     2461 gcataaaacc aacgagaagc gaatacatca agattgttac cctctaaatc aatatcatca
     2521 aacaatggtt cgatttgttt atgcacatcc tctacgttga tcggattaaa aaacactctc
     2581 cccaacatct gcgaaagcgt ttccctcaat gccggataaa gcgtagaacg atttaatcgg
     2641 tttttatatc catcttcctc ttccattttc attttataga ggtaggtttg acccgcttgt
     2701 cgcatttttt gtgtgccacc gagtaaatca tcgatgattt ttgtttttgc ttttaatgcc
     2761 gcaaaatcgg ctgagatgga gttaggcatt gttacctcat tttaataaac ctttaatgca
     2821 cattgcttcg cctcgcctct tgcctgaata agaggattta aggcataacg aatggcatca
     2881 atatagtggt tatgagcgtc aataatgata ggcagtacat caccacttaa tctgtccgtt
     2941 ttgtaactat aaaggcgaaa ttctttcaat acctctgtac aacttggatg aatatatact
     3001 ttacgataag attttatatg ctcaacccca tcttcgactg agcctttcca ctttggaaca
     3061 ggttgaatat aaggcaatcc atgtcgtttt aaataactga tggattctgg gcgagcagaa
     3121 tctgctctaa cagtataatt actcaaatca ggcaaagcat ttgctaaata agcagcggtc
     3181 tcatcgagct ctagtcttac tttccctgct tcacgctcga tatataagtc atcatcgaac
     3241 acccaacatt tcacacaggc tgttgggtca ttggcaaaac caaagtccag cccgtaataa
     3301 ggaccgttaa aatcagggtt aggcttaaat tctaattctt ggtatttatc tctaaagatt
     3361 tgagcatcag aggattctaa gtagtcccc
//
LOCUS       DANLUA010000212         3385 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLUA010000212 DANLUA010000000
VERSION     DANLUA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1789
                     /gene="frdA"
                     /locus_tag="PXB44_002368"
     CDS             <1..1789
                     /gene="frdA"
                     /locus_tag="PXB44_002368"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL5282797.1"
                     /translation="FDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRSHTVAA
                     EGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGCPWSRR
                     EDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLDILTDN
                     GEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAYRHGVA
                     LRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIGKPENK
                     YMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFICELAKA
                     YEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANRLGSNS
                     LAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESWSDIRN
                     QMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKIELGFI
                     LDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYSDVKIT
                     KSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            1805..2536
                     /locus_tag="PXB44_002369"
     CDS             1805..2536
                     /locus_tag="PXB44_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL5282798.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            2546..2938
                     /gene="frdC"
                     /locus_tag="PXB44_002370"
     CDS             2546..2938
                     /gene="frdC"
                     /locus_tag="PXB44_002370"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL5282799.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            2952..3299
                     /gene="frdD"
                     /locus_tag="PXB44_002371"
     CDS             2952..3299
                     /gene="frdD"
                     /locus_tag="PXB44_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL5282800.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
BASE COUNT          984 a          629 c          820 g          952 t
ORIGIN      
        1 ttttgatgtc gcgattatag gtgccggtgg tggtggcttg cgtgctgcta ttgcggcggc
       61 tgaagcaaat ccaaatctaa aaattgcttt aatttcaaaa gtttacccaa tgcgtagcca
      121 tacggttgca gcagaaggtg gttcagcggc ggtaattaaa gataccgact ctttcgacaa
      181 tcactttaat gataccgtcg gtggtggtga ctggttatgt gagcaagata ttgtggagta
      241 ttttgtagag cattcgccga ttgaaatgac acaattagaa cgctggggtt gcccttggag
      301 tcgtcgtgaa gacggtgaag tgaatgttcg ccgttttgga ggaatgaaaa ttgagcgtac
      361 ttggtttgcg gcggataaaa ccggtttcca cattttgcat actttgttcc aaacttcgat
      421 taaatatccg aatattgtgc gttttgatga gcattttgtg ctggatattc tcaccgacaa
      481 cggcgaagct cgtggttgtg tggcaatgaa tatgatggaa ggcacacttg ttcaaatcaa
      541 tgcaaatgca gttgtgattg caaccggtgg cggctgccgt acttatcgct ttaacactaa
      601 cggcggtatt gtaaccggcg acggtttatc aatggcgtat cgtcacggtg tggctttgcg
      661 tgatatggag ttcgtacaat atcacccaac aggtttgcca aataccggta ttttgatgac
      721 agaaggttgt cgtggtgaag gtggtatttt agttaataaa gatggctacc gttacctgca
      781 agattatggt ttaggaccgg aaacacctat cggcaaaccg gaaaacaaat atatggaatt
      841 aggccctcgt gataaggttt cacaagcatt ctggcaagaa tggcgtaaag ggaacacctt
      901 aaaaaccgca aaaggcgtgg acgtagttca cttagattta cgccatttag gtgaaaaata
      961 tttacttgag cgtttaccgt ttatttgtga attagcgaaa gcctatgaag gggtcgatcc
     1021 ggctaaagcg ccgattcctg ttcgcccggt ggttcactac acgatgggtg gtattgaagt
     1081 ggatatgaat gcggaaacct caattaaagg cttattcgca gtaggtgaat gtgcttcatc
     1141 aggcttgcac ggtgcgaacc gtttgggttc taactcatta gcagagcttg tggtgttcgg
     1201 caaagttgcc ggtgaaaatg cggctcgccg tgcgttagaa gtgactgctc gcaaccagcc
     1261 acaaattgat gcacaagcaa aagatgtggt ggctcgcttg cacgcattag cgaaccaaga
     1321 gggtaatgaa tcttggtcgg atatccgtaa ccaaatgggt gatgcgatgg aagaaggttg
     1381 tggtatttac cgtacccaag aaagtatgga agcgactgta aacaaaatcc aagagctaaa
     1441 agagcgttat aaaaacatca gcgtaaaaga taaatcaagt gtgtttaaca ctgatttact
     1501 ctacaaaatt gaattaggct tcattttaga tgtggctcaa tccattgctt gctctgcggt
     1561 agagcgtaaa gaatctcgtg gggctcacca acgtttagac tatgttgagc gtgatgatgt
     1621 gaactactta aaacacacac aagcgtacta caatgcagag ggtacaccga cgattaaata
     1681 cagcgatgtg aaaatcacta aatcacaacc ggctaaacgt gtgtatggtg cagaggctga
     1741 agcccaagaa aaagtcaaaa aagcagcaga agctcaagca caacaataga gaaggagtag
     1801 taaaatggca aatttagata aaatgaccat cgaagtgcta cgctacaacc ctgaatcaga
     1861 tagcgagcca catttagaca gatatgaagt gccgtatgac agccaaactt cacttttaga
     1921 tgcattgggc ttcattaaag acgaattaga accggaactt tcttatcgct ggtcttgccg
     1981 tatggcgatt tgtggttctt gcggaatgat ggtaaacggt aagccgaagt tagcttgtaa
     2041 aacattcctg cgtgattaca gcggttttat gcgaattgag ccgctggcga acttcccgat
     2101 tgagcgtgac ttagtagtgg atttaagcca ctttattgat agcatcgagg caattaaacc
     2161 ttatattatt gataataaag caccggaagg acagcgtact aaacaaacgc cggcacaatt
     2221 agagaaatat cgtcaattct caatgtgtat caactgcggt ttatgttatg cggcttgccc
     2281 acaatttggt ttgaaccctg aatttatcgg acctgctgcg attacattgg ctcaccgcta
     2341 taacttggat aaccgtgata atggtcgtga agagcgtatg aagctactca gcagcaaaaa
     2401 cggtgtttgg agctgtactt tcgtgggtta ctgctcggaa gtctgtccga aacacgtcgg
     2461 ccctgcttcg gctgtaaacc aaggtaaact tgaaagtgcg aaagattatg ttatctcaat
     2521 gatcaaaccg agataggagg aagaaatgtc agcaacaaaa cgtagacctt atgtaagggg
     2581 aatgaaagcc agctggtgga aaaaactcga cttctataaa atgtatatgg tgcgtgaagc
     2641 tacctgtctt ccaactgttt ggttctgcat tgtattattt tatggagcag ttgcgttaag
     2701 taaaggaaca tttactaccg attttgtgga tttcttacaa aatccgttag ttgttatcct
     2761 aaacataatt tcattggcag caatgttata tcatgcggca acactttttg tgatgacacc
     2821 tgccgtcatg tccgtgatgg ttaaaggtaa acacttacca gtctcggttg gaagaaatat
     2881 tctttgggca gcaacaggcg taattagcgt aattgcatta atcttggttt atatttaaga
     2941 aggggaagaa aatgagtctt gatcaaaacc caaaacgttc aaatgaaccg ccggtatggt
     3001 tgctttttag tgctggggga atggtaagtg cattattttt ccctgtggta atctttatta
     3061 tcggtttatt actgccattc gggttagtat caccagataa tatcatttcc tttgcccata
     3121 cttggatcgg caagctcgct attatggtat tagcgatttt ccctatgtgg gcaggaatgc
     3181 accgtatcca tcatggatta cacgacctga aagttcacac gccagcaggt aatgtgattt
     3241 tctacggatt gtctatctta tacacaattc tagtaatcat cgcagtcgtg cagatttaaa
     3301 tgagaaagac catcttgtat aaagcagatg gtcttttcat ttttaatatg ctaacaagcg
     3361 gtcgaattta taagaaaatt tgcaa
//
LOCUS       DANLUA010000213         3365 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLUA010000213 DANLUA010000000
VERSION     DANLUA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(272..583)
                     /locus_tag="PXB44_002372"
     CDS             complement(272..583)
                     /locus_tag="PXB44_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282801.1"
                     /translation="MTKTKHKHPLAEAFYQLGKDVGEINRRLVVVDEQSLKLQELKQT
                     INEQTEQIKTLGTVVNKLIDALQQSQSLNVRSESAVAATENQDHLNDGVQYQGPGRAR
                     A"
     gene            complement(576..725)
                     /locus_tag="PXB44_002373"
     CDS             complement(576..725)
                     /locus_tag="PXB44_002373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282802.1"
                     /translation="MDNILITIHPTETIDITIDKLVVIHIGEEYGDTPDFLTIYQQAK
                     ELNDD"
     gene            complement(959..1252)
                     /locus_tag="PXB44_002374"
     CDS             complement(959..1252)
                     /locus_tag="PXB44_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282803.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGEESDSEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYNHLKETGQIVV"
     gene            complement(1375..>3365)
                     /locus_tag="PXB44_002375"
     CDS             complement(1375..>3365)
                     /locus_tag="PXB44_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL5282804.1"
                     /translation="FTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVATDWTP
                     APEDVDSAVSAVSAKIDTIQETLANADSALSSRIDTVNASVGSNSSKITQVSNAVASV
                     DGKLSATHTIKTETIAGGRKAIAGIAFGATADNRNAESSVIVMADKFEVVKNAQDGTP
                     VRLFGVVENKVAINGNLIATGSISGDHINANSVRTAVLTAGAIKTEHLAAGQISADKL
                     AIGLGGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRTIFNRNEQDNYGLKNG
                     GLPNELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAKLVIEN
                     IEKPGGAYLGRIGNSEVITGYSAHQGEFKNMKRVKVVFTAPTSGCIRFALRLDNIQGK
                     ANPFLFARRPMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAANTITA
                     NEIATGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGTTI
                     TGNTISGGSINGTTISGTTISGGTIKGARLEGATGKFTGELEVSQLIGGGVIEQVTGK
                     LKYSHRNRIYEGQEHGENSSSPIYRHEYIYKATVTINAAPTRRYIEIIGSESFILEAN
                     QSITKTIEKSLNRNASGSSLDFIVLAYSISTAKYISIS"
BASE COUNT          890 a          724 c          652 g         1099 t
ORIGIN      
        1 gtgtccgcga atgggtggct ggtgaacgta aaatcccttt cggggtttgg gctgatttga
       61 ccgaattggt taaagccaaa aaagctaact tatcatcaat tcttaaaaaa ttaacaatag
      121 attagcactg attttaaagc ccctctcggg gcttaattat agaaaataaa atcacaatgg
      181 ctaattttgc caaatttgcc tgttaaaacg tgcgaaaaat tacaaaaaac ggctagagct
      241 atagccgttt tgagtgcaga tgttaacaga gttaagctct agcacgtcct gggccttgat
      301 actgcacacc atcatttaaa tgatcttgat tttcggttgc tgctacagca gattctgatc
      361 ttacatttaa actttgtgac tgttgtaatg catcaataag tttatttacc actgttccga
      421 gtgttttaat ttgctcggtt tgctcattaa tagtctgctt taactcttgc agttttaatg
      481 attgctcatc cacaaccact aatcgacggt taatctctcc tacgtcttta cctaattgat
      541 aaaatgcttc tgcaagtggg tgtttgtgtt tagttttagt catcgttaag ctcctttgct
      601 tgttggtata tagttaaaaa gtcaggtgtg tcaccatact cctcacctat atggataact
      661 accagtttgt ctatggtgat atcaatagtt tcggttggat gtatagtgat taagatattg
      721 tccatattcc cgccttaaat ttgggtttgg tcagggataa gttggatcgt gccgtagtac
      781 tgccattttg ggtggatagc tcaatttgag gctctgtgct gccctctact atggcgtgta
      841 aatccagcct aataccctct aaatttacag gctcgccatt tgtttgccta aaaaaaaagg
      901 cgataactta cgtcatcgcc tctgaaaaac ttaaatttat gcttttgcat ttagacccct
      961 acactacaat ctgaccggtt tctttgaggt ggttgtagat acgtcctaac ataatctgcg
     1021 ttggcgtttt gccaatatcc tcttttgtta gcggtgcatc agtaatcgct ttggctgatt
     1081 gagcatcaat atatttgtac tcacttgtga tgattacaaa gtttgttacc tcgctatcgc
     1141 tctcctcacc tgtgccaacg acatatttag cattgagtga gccgtcgtct tgagttgagt
     1201 agcttgcgat ggtggaatac attgggttta agattttgtt aaaagttgtc ataatttttc
     1261 cttttatcga tagttgaaat aaaaacaatc gcctgaattg acctgcaagc ggttggtttt
     1321 ggatatttaa aaaagctgtg gctatccgca ctcagtacta cattgaggcg ataactaact
     1381 gatagagata tatttagccg tcgaaatgga ataggctaaa acaataaaat ctaaagagct
     1441 accacttgca tttctattta atgatttctc tattgttttt gtgattgatt gatttgcttc
     1501 taaaatgaat gactcacttc cgatgatttc aatatacctc cttgtcggcg ctgcatttat
     1561 ggtgacagtt gctttataga tatattcgtg tctataaatt ggagaggagg agttttctcc
     1621 atgttcttgc ccctcataaa tgcgattacg atgtgaatat tttaatttgc ctgtaacctg
     1681 ctctataaca cctcccccta ttaattgaga tacttcaagt tcgccggtaa atttgccagt
     1741 agccccctcc aatcttgcac cctttattgt gccgccagag atagttgtac cgctaattgt
     1801 tgtaccatta atacttccgc cactaattgt attaccagta attgtcgtac ctgtaattgt
     1861 accagcagtt accttaccaa gatttgccga tattgccgct aaattgttta catttagctt
     1921 atccgcagtg atcgaactcc ccacaatatg acttgcatta atcgagtttg ctgccatatt
     1981 tcgagctgca atcgtgccgg ttgcgatttc gttggcggta atggtatttg ctgcaatttg
     2041 ttgagcagta acggtattag taactattga gccaccgtgg atagcggtta caccggcatt
     2101 ttgccaaggg ctaggctgag tgatatctgt aacattatca ctagcctcct caagcattgg
     2161 tctacgagca aataaaaatg gattagcttt accttgaata ttatctaatc gtaatgcgaa
     2221 acgaatacag cctgaagtag gtgcagtaaa tacaacctta acccgtttca tatttttaaa
     2281 ttcgccttga tgagcactat agccagtaat tacctcgctg ttgcctatcc gacctaaata
     2341 agcaccacca ggtttttcga tattctcaat gactaattta gcttgcccac gatgacaagc
     2401 taaccatacg gaggcaatat attttttatt aggcacaaca ttaatatcct gagataacca
     2461 cccaattcgc ccagtatcag aggtttcctt tgtagtgcta taccgcatcg aaaatactaa
     2521 ctcatttggt agtcctccat tttttagtcc gtaattatct tgttcattcc tattgaaaat
     2581 agtacgggtg tcaatattta gccgatcacc aacttctcct cgccagtagt tccagccaaa
     2641 cggagctatt gacaaatcgg gtgttgcaaa aataggatta accagcaaat tcccacctag
     2701 cccaatcgct aacttatccg ccgatatttg ccctgctgcc aaatgctccg ttttaatcgc
     2761 tcctgcagtc aatacagcag ttctgacgga atttgcattg atgtggtctc ctgaaatcga
     2821 gcctgttgcg attaaattcc cattaatcgc tactttattt tctaccacgc caaatagtct
     2881 tactggcgtg ccgtcttggg cgtttttcac gacctcaaat ttatctgcca tcacaatcac
     2941 agaagattcc gcattacggt tatctgccgt tgcaccaaat gcaataccgg caatcgcttt
     3001 gcgtccaccg gcaatggttt cggttttgat ggtatgggta gcagataatt tgccgtccac
     3061 acttgctacc gcattgctga cttgggtaat tttagatgag tttgatccga ctgaggcatt
     3121 gaccgtatca atgcgagagc ttaatgcaga gtcggcattc gctaaagtct cctgaatagt
     3181 atcaatttta gccgaaactg cactaaccgc actgtccaca tcttcaggtg ctggcgtcca
     3241 gtctgtggct acgttgcctt tttcaagttt gacgctgtga aacgctaacc aatctgtggt
     3301 ctcataagta ccatttacac caacaagcag ataggctcgc tcacttgtcc attcggcagt
     3361 aaagg
//
LOCUS       DANLUA010000214         3348 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLUA010000214 DANLUA010000000
VERSION     DANLUA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(101..223)
                     /locus_tag="PXB44_002376"
     CDS             complement(101..223)
                     /locus_tag="PXB44_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL5282805.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(303..1280)
                     /gene="rluD"
                     /locus_tag="PXB44_002377"
     CDS             complement(303..1280)
                     /gene="rluD"
                     /locus_tag="PXB44_002377"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL5282806.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1421..2200
                     /locus_tag="PXB44_002378"
     CDS             1421..2200
                     /locus_tag="PXB44_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL5282807.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2273..3304
                     /gene="tsaD"
                     /locus_tag="PXB44_002379"
     CDS             2273..3304
                     /gene="tsaD"
                     /locus_tag="PXB44_002379"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL5282808.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
BASE COUNT          995 a          716 c          657 g          980 t
ORIGIN      
        1 gcggtaaaat ttaccgtatt tttcgcaaaa atatacctta ttgaacaatt gttcaataag
       61 gtatatatat tattagcttc ttagaattcg aagctttttg ttattgtgca gcctgctgaa
      121 ttttatcgcc cgctttttca acatcctcac caacaccatc aacggtatta cacgcagtca
      181 agaaagttgc agctgctaaa atagttaata ctaatttttt catagaaagc tccttttttg
      241 ttgtttactt atgaaaacga tttcaatata gcaactttac attatttaag caacacctaa
      301 acttaataat ctaattgatc tttataaagc tcataatccg cttttagtgc ctgaatcaat
      361 tcaataaaat catcaggtaa cggggcgtgc cactccatca tttcaccagt aatcggatgc
      421 tctaatctca acattgtggc gtgtaaggct tggcgttgga agcctcgtaa caccgataaa
      481 aacgagtcac tcgcaccttt aggtgggcga ggtctaccgc catagagctg atcacctaat
      541 aatgggtgag caatatgcgc catatgtacc cgaatttggt gagtacgccc tgtttctaat
      601 cttaaacgta aacgtgtgta attacgaaaa cgctccataa tacgataatg cgtaaccgcc
      661 ggttttccca ttgggtgaac agccattgcc gtacgtttag ttgggtggcg agccattggc
      721 tcatcaactt ttccgccctg tgtcataatg ccgctggcaa tcgcttcata ttcacgggta
      781 atacgacgtt tttgtaatgc ggttactaag tgagtctgtg ccggaatgtt tttagccact
      841 accattaagc ctgtcgtgtc tttatccaat cggtgaacaa tccctgctcg aggcacttct
      901 gcaatcggtg gataataatg tagcaaagca ttcagcactg taccgtccgg attaccagcc
      961 cctgggtgaa ccactaaatc cttcggttta ttaatgacga taatatcgtc atcttcatac
     1021 acaatattta gaggaatatc ttgaggctca aaacgaattt cctcttctat ttcagcggta
     1081 atttcaacag tttcaccacc aaatactttt tcccttgctt tattcacgat attgccatta
     1141 actttcacta gatcactttc aatccacact ttaatgcgtg aacgagaata gtccggaaaa
     1201 agttgtgcca gagcctgatc taggcgagca cctagtaaat ctgcagtaat ttctgctgtt
     1261 aaaatctttt gttgagtcat taaaccataa atcctaatta gtcatttgct tacttgcgtg
     1321 atgttatata gaatagcgag atgaacttca taagttattc ttagtcagag gacattgtac
     1381 attaactttt cgtcctagac actttttata ggtaaaacat atgcgtaaat ttaaatctct
     1441 tgccacatta gtattagccg gactgtttgt tgcaggttgt tcaaattcaa ataaagaatt
     1501 agaacaatcc aacgttcaag atctttatgg caaaggtcag acctatttac aagatggaga
     1561 ttataattct gccattcgct acttagaagc ggttggaacc aaaggaggcc aatattcagg
     1621 acttggtgag caaacccaac taagtttaat ttatgctcaa tataaagttg gcgaatacta
     1681 taaagcactt gatgccgcag aacgttttgc tcgctcttac ccgaatgcag ctaatatgga
     1741 ttacgtattc tatctagcag gattatcaaa tgcacgtcta agtgataatt ttattcaaga
     1801 cttctttggt gtaaatcgcg cctctcgagc cgtagataat gttcgtaatg cttacggtaa
     1861 cttccaaact attgtacagc gctaccctca aagccaatat gcacaagatg ctcaaaattg
     1921 gatgaattac ttatttaacc gtttagcgga acacgaatta gccgttgtta aattttatga
     1981 tgaacgtaat gcctatgtgg ctgtagtaaa ccgtatcgaa gaaatgttgc gtttttaccc
     2041 gaatacactg gcaaccaaac aagcattgcc atacttaaaa gaagcatata agcaaatgaa
     2101 tatcccagat gcagagcaaa aaacggaact cttgatccaa gaatatgaaa gcaaagagct
     2161 accgaatcct gaaaaactga agtatggcga acaattctag tcgtacagag aataatgtga
     2221 ggggcgttct tcgccccttt tggttttcta acttattttg acttctccaa ctatgcgaat
     2281 tttaggtatt gaaacctctt gtgatgaaac cggtgttgcc atttatgatg aagacaaagg
     2341 cttagtggca aaccagcttt atagccaaat tgatatgcac gccgattacg gtggcgtagt
     2401 ccctgaactg gcttctcgag accatatccg taaaacgttg ccactaattc aagaagcctt
     2461 aaaagaggcc aatctgcaac cctcggatat tgacggcatt gcctatactg ccggcccagg
     2521 cttggtcggg gctttattgg tcggctcaac cattgcccgt tcgctggctt atgcttggaa
     2581 tgttccggca ttgggcgttc accatatgga agggcattta cttgccccaa tgttggaaga
     2641 aaatgcccct gaatttccgt ttgtggcatt attgatttca ggtggacaca cccaactggt
     2701 aaaagttgac ggcgttgggc aatacgaact actcggggaa tcaattgatg atgctgccgg
     2761 tgaagccttt gacaaaacag gcaaactact cggtttggat taccctgccg gtgtagcgat
     2821 gtcaaaatta gccgaatccg gcacgccaaa tcgttttaaa ttccctcgtc caatgaccga
     2881 cagaccggga ctggatttca gtttctccgg tttaaaaacc tttgctgcga atacgattaa
     2941 agccaatctt aatgaaaatg gtgaactcga tgagcaaacc aaatgcgata ttgcccacgc
     3001 attccaacaa gccgtggttg atactatttt aattaaatgc aagcgagcgt tagagcaaac
     3061 cggctataaa cgcttagtaa tggcaggcgg cgtaagtgcc aataaacaat tacgagcaga
     3121 ccttgcggaa atgatgaaaa aattaaaagg cgaagtattc taccctcgcc cacaattttg
     3181 cactgacaac ggcgcaatga ttgcctacac tggctttctt cgcttaaaaa acgatgaaca
     3241 aaccgactta agcattagcg taaaaccccg ctgggctatg accgaattac caccgattaa
     3301 ttaacctttc aagcggtgaa atttcttgtt aattttgcaa aaatttaa
//
LOCUS       DANLUA010000215         3333 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLUA010000215 DANLUA010000000
VERSION     DANLUA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            41..2017
                     /gene="cpdB"
                     /locus_tag="PXB44_002380"
     CDS             41..2017
                     /gene="cpdB"
                     /locus_tag="PXB44_002380"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL5282809.1"
                     /translation="MLNRRKFIQLGATAMLVLGTQPSAFAQTAKNKAGLRVIATTDIH
                     GFLTDFDYYKDAPTEKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKG
                     AKEGKPEPSIMALNAMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDE
                     PFFTPYVIQQKEIVDEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQK
                     YVPIVKKEGADIVVALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNK
                     EFANSPNADIAKGTMKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYN
                     TEEKKATVENHPEIVALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQ
                     AQKAYVENVVKGLPELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYL
                     YPNTLVVVKATGAELKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSY
                     QFDITQPARYDGECKLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTG
                     DKHIVFAAPDENRQVLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPT
                     DKAKAFIKENAQYPMEYIENDETGFAVYRIDLTK"
     gene            complement(2090..>3333)
                     /locus_tag="PXB44_002381"
     CDS             complement(2090..>3333)
                     /locus_tag="PXB44_002381"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL5282810.1"
                     /translation="SQHDVVSEYTQFISDTLNIEDSGEHIAQLHQLGYLPIRIKAIPE
                     GKSVAVKVPLMTIENTHSSFFWLTNYLETLINVSLWQPITSASIAFAYRKALAEFSEQ
                     TCDDNSHLPFQAHDFSMRGMSSLESAESSGAGHLTSFLGTDTIPAISFVTAYYGSQHL
                     IGTSIPASEHSVMSSHGVDELPTFRYLMAKYPNNMLSIVADTTDFWHNITVNLPLLKQ
                     EILARPESAKLVIRPDSGDFFTIICGNNSSPVEHERKGLIECLWDIFGGSINQKGYKV
                     LNPHIGAIYGDGVTYDKMIRILEGLTAKGFASSNIVFGVGAQTYQRNTRDTLGFAIKA
                     TSITINGVEKAIFKAPKTDNGLKKSQKGRVKVISYDHYVDELTALDDFSDDLLEVVFE
                     NGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1091 a          643 c          680 g          919 t
ORIGIN      
        1 ttttacaatt taccctttca tttcattaag gagatatttt atgttaaata gaagaaaatt
       61 tatccagctt ggtgcaactg caatgcttgt attggggaca caaccgtctg catttgcaca
      121 aacagccaaa aataaagctg gcttgcgtgt catcgccact acggatattc acggtttctt
      181 aactgatttt gattattaca aagatgctcc aacagaaaag tttggtttta cccgtacggc
      241 aagcctaatt aaacaagcta gagcagaagt taaaaacagt gtgttagtcg ataatggtga
      301 cttaattcaa ggtaatccta tttctgacta ccagtcaacc aaaggtgcaa aagagggcaa
      361 acctgaacct tcgattatgg cattaaatgc gatgcaatat gaagtcggaa cgctgggaaa
      421 tcatgaattt aactacggtt taagctattt agataatgct attaagcaag caaaattccc
      481 tattgtcaat gcaaacattg tgaagccggg gacagatgaa ccattcttta caccttatgt
      541 tattcaacaa aaagaaattg tagatgaaaa agggaataag cataccatta agattggtta
      601 tatcggtttt gtaccaccac aagtgatgat ttgggataaa gctaatcttg aaggaaaagt
      661 tgaagctcga gatattgtga agaccgcaca aaaatatgtg cctatagtaa aaaaagaagg
      721 ggcagatatt gtcgttgcat tggcacatac cggcccttca gacgaaccct ataaagaagg
      781 cgctgagaac tgtgcgtttt atctagctga tgtaaaaggt attgatgcag ttattttcgg
      841 gcattcacat cgcctgttcc caaataaaga atttgcaaat tcaccaaatg ccgatattgc
      901 aaaaggaaca atgaaaggcg taccggaaag tatggctggc tactggggaa ataacatcag
      961 tgtgattgat ttaagcttgg ccgaaaacga tggtaaatgg ggcgttgtgg acggtagagc
     1021 agagttgcgt ccaatttata atacggaaga gaaaaaagca accgttgaga accaccctga
     1081 aatcgttgca ttattaaaag aaacccatga agcaacccgt caatttgtgt ctcaacctat
     1141 cggtaaagca actgataata tgtatagtta ccttgcgtta gttcaagatg accctaccat
     1201 tcaaattgtg aaccaagctc aaaaagcgta tgtagaaaat gtggtaaaag gcttgccgga
     1261 gcttgcagga ttaccaattc taagtgccgg agcaccgttt aaagccggtg gacgtaaaaa
     1321 tgacccgaca ggctataccg aagtcgataa aggcgaatta actttccgca atgcggcaga
     1381 cttatacctt tacccaaata ccttagtggt ggttaaagca acaggagcag aattaaaaga
     1441 gtggttagag tgcagtgcag gaatgttcaa acaaattgat gtcgcttcgg ataaaccgca
     1501 atatttactg gattggacag gtttccgcac ctataactac gatgtaattg acggtgtcag
     1561 ctatcaattt gatataaccc aaccggctcg ttatgacggc gagtgcaaat tgatcaaccc
     1621 taattcgcac cgtgtggtga acttaacctt tgacggcaaa ccggttgatc ctaaacaaga
     1681 attcttaatt gccaccaaca attaccgtgc ttatggagca aaattcccgg gaacaggcga
     1741 taaacatatt gtgttcgccg caccggacga aaaccgccaa gtgttagcga attatattac
     1801 caccgaaagt aaagctcacg ggcaggtaag tccgaaagcg gataacaact ggcgtattgc
     1861 accaattagc tcaaatgcaa aattggatat tcgttttgaa acctctccaa cagataaagc
     1921 taaagctttc attaaggaaa atgcacaata tccgatggag tatatcgaaa acgatgaaac
     1981 cggcttcgca gtttaccgta ttgatttaac caaataatca gagctaaata caagcggtct
     2041 aatttgcaaa aaaattaaca aatttgaccg cttgtttcat tccatttttc taaaattgtt
     2101 gtgctatatt ttgccgaatt tcatcaaaat tggtcgattt gaccaactta ccattttcaa
     2161 aaaccacctc taaaagatca tctgaaaaat catccagtgc ggttagctcg tcaacataat
     2221 gatcataaga gattactttt actcgccctt tttgcgattt ttttaagccg ttatcggttt
     2281 taggagcttt gaaaattgcc ttttcgacac cgttaattgt aattgaagtc gctttgattg
     2341 caaaacccag cgtatcacga gtattgcgtt gataagtttg agccccaacg ccaaacacaa
     2401 tattgctgga agcaaaacct tttgccgtta agccctctaa aatccgtatc attttatcat
     2461 aagtgacgcc gtcaccataa attgccccaa tatgcggatt aagcacttta taaccttttt
     2521 ggttaatact accaccaaaa atatcccaca aacactcaat cagacctttg cgttcgtgtt
     2581 caacggggga ggaattgtta ccgcaaataa ttgtaaaaaa atcaccgcta tcagggcgga
     2641 taaccaattt tgctgattcc ggtcgagcta aaatttcttg ttttaataat ggcaaattaa
     2701 cggtgatgtt atgccagaaa tcggttgtat ctgccacaat agatagcata ttatttggat
     2761 attttgccat taaataacgg aaagtcggca gctcatcaac accgtgtgaa ctcatcaccg
     2821 aatgttctga tgccggaata gatgtaccga ttaaatgttg agaaccataa taggctgtca
     2881 cgaatgaaat tgcaggaatg gtatctgtgc ctaaaaatga ggttagatgt ccggcaccgg
     2941 agctttctgc tgattctaag gaactcattc cacgcataga gaaatcgtgt gcctggaagg
     3001 gtagatggct attatcatca caggtttgct ctgaaaattc ggctagagcc ttacgataag
     3061 caaatgcaat ggaagctgat gtaataggct gccataatga cacattaatc agggtttcca
     3121 aataattagt cagccaaaaa aagcttgagt gagtgttttc aattgtcatt aaaggaactt
     3181 tcactgcaac agattttcct tctggaatag ctttaattct gataggtaag taaccgagct
     3241 gatgcaattg tgcaatatgt tcgccagagt cctcaatatt caatgtgtca gaaataaatt
     3301 gagtatattc agaaacaacg tcatgttgtg agc
//
LOCUS       DANLUA010000216         3323 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLUA010000216 DANLUA010000000
VERSION     DANLUA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2074
                     /locus_tag="PXB44_002382"
     CDS             <1..2074
                     /locus_tag="PXB44_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL5282811.1"
                     /translation="FFSKIIKIPNLKGFLPHCEIRRRPLVGKTFIAQKRPNQIIGWGL
                     RPSAVGSRAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDTTCPSRL
                     EKLLLEKPLQDQEQIKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLVIDQTFG
                     DMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQYTDRVR
                     IITEDVNPASLIKLCEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDDRNEAVQ
                     LLVQQNRRKDRSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLNQRLSGN
                     LYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGKADVLQF
                     AQQNNIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQILLSAEHF
                     SETDLSYAETLRQKLVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASIKSGSPQ
                     IKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDNANILDC
                     ILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSLTLSQLI
                     AATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKLQHLYRA
                     FKYASHTK"
     gene            2097..>3323
                     /locus_tag="PXB44_002383"
     CDS             2097..>3323
                     /locus_tag="PXB44_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5282812.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAH"
BASE COUNT         1060 a          539 c          685 g         1039 t
ORIGIN      
        1 gttttttagt aaaattataa aaatccctaa tttaaaaggt tttttacctc attgtgaaat
       61 taggcgaaga cctcttgtgg gaaaaacctt tattgctcaa aaaagaccga accaaatcat
      121 cggctggggg cttcgtcctt cagcggtggg atcaagagct tatgcacaaa aacatcaact
      181 tccctatatt gcattagaag acggcttttt acgctcacta gggctgggtg taaaaggcta
      241 tccgccattt tctttagttt atgatgatat aggtatttat tacgatacca cctgtccttc
      301 tcgtttagaa aagttattgc ttgaaaaacc tttacaggat caagagcaaa tcaagcttgc
      361 agagagagca ttatctcaaa tcttaactta cggactttct aaatataatc acgcactaga
      421 ttttagtgaa atcccctctg ccaaagaaaa aatcgtgtta gttattgacc aaacctttgg
      481 tgatatggcg gtgaaatatg gtcaagcgag tgaagtggat ttcaataaaa tgttggaaac
      541 ggcaatcagt gagaatccag aggctcaaat ttgggtaaaa actcaccccg atgtattaag
      601 tggcaagaag aaaggctatt tgaccgattt acctcaatat actgataggg tcagaattat
      661 tactgaagat gtgaatccgg caagtttgat taaattgtgt gagaaagtct attgcgtaac
      721 ttcgcaaatg gggtttgagg cattattatt aggtaaaaaa gtggttacct ttggtgtacc
      781 gtggtttgcc ggttgggggg taacagatga tcgtaacgaa gcggttcaac tattagttca
      841 acaaaataga cggaaagatc gttcattact gcaacttttt tatgctgcgt attttgatta
      901 cagccgttat attaatccga ataccggtga gaaaggctct atttttgatg tcatcgatta
      961 tctatataaa gccaaaagat taaatcagcg cttaagtggg aatctatact gtattggtat
     1021 gtcgctatgg aaacaggcgg taattaagcc gttttttaat ttgccacaat gtcgattaca
     1081 ttttgttcgt tcgctaaatt ctttaaaaaa acgaccgctt gcagataatg caaaaatttt
     1141 agtttggagc aatggcaaag cggatgtctt gcaatttgca cagcagaata atattccatt
     1201 gttaagaatg gaagatggat tcattcgctc tgtcggacta ggcgctaatt tagttgcacc
     1261 gctttcttta gtagtcgatg atttaggcat ttattttaat gcagagaaca cttcacgatt
     1321 agaacaaatt ttattgagtg cagagcattt ttctgaaact gatttgtctt atgctgaaac
     1381 attaaggcag aaattggtgg ctagccatat tggaaaatat aatgtgggcg gtggcgatgt
     1441 aaaatttaat gctcaaggta aaaaaataat tcttgtaccg gggcaggtag aagatgacgc
     1501 ttcgatcaaa tcaggttctc cacaaattaa aagaaatctg gatttactga aaaaagtgag
     1561 ggaattaaac cctagtgcct atattattta taagcctcac cctgatgtgg taagtggtaa
     1621 tcgagtcggt cagattccta cggcagaaac acgcaggttt gcggatcaga tggtggataa
     1681 tgccaatatt ttagattgta ttttaattgc agatgaagtg catacaatga catctttagc
     1741 cggtttcgaa gcattattaa gaggtaaaaa agtgcattgc tacggattac ctttttatgc
     1801 tggttgggga ttgacggaag atagattgaa gtcggcacgc agaggtaaat ctttgacatt
     1861 atctcagtta attgccgcaa cattgatata ttaccctctg tatgttgatc ctataagcag
     1921 gcagtttatt gatgccaatc aagcgattca tatattgtca caacagaaac gaaacctaac
     1981 caatcaaagg attaaacgtt cttgggtgag taaacaatat ggaaaattac agcatcttta
     2041 tcgtgcgttt aaatatgctt cccacactaa ataagcgtag agggtaggaa attaagatgg
     2101 aacattattt agatgagcta atcatcaata gccagagaat tttattgtta caaggcccga
     2161 taggctcttt tttcactgag tttgcccgtt ggttagaaag tgagcaaaaa gaggtcttca
     2221 agttaaattt taatggcgga gatgaatatt tctatccaaa taatagcccg aatacttttc
     2281 cttactatga tagcttagat aattttgaag cctatttaat tcagttttgc caaactcatc
     2341 aaatagataa tatcttatgt tttggcgata atcgttatta tcataaagtg gctaaaaacg
     2401 tttgcttgaa gttaaagttg gtcttttggg cgtttgaaga agggtacttt aggccagaat
     2461 atattacgtt ggaaaaatgg ggtgtaaatg ctttttctcc aatccctaaa aatcacactt
     2521 tctttttgcc ttatgccgag ttaccagtag tcagtgaacc agaaaaagta tctaaaggct
     2581 ttagatgtat ggcgggaaaa gcaatcccgt actattggaa tgcctataaa aaacgtaaag
     2641 catacgcaaa atatcagcat catcgttttc tgaatctcaa atactacctt aatttatgga
     2701 caaggtcggg aattaagcga atatgttatt ggttacatga taaaggcttt gccaagcggg
     2761 tagaaaaagg agagtttggt gaatttttca ttgtgcctct acaagtttat gatgacactc
     2821 aagtgaggat tcatagtgaa caaaaaagtg tagagcgttt tttaagggaa gttctgaatt
     2881 cctttgccaa atttgcaccg gcacatctca atttaattat taagcatcac ccgatggata
     2941 gaggatttat agattataaa atggtgattt atgaattctt agagcaatat ccccaattaa
     3001 acggtagagt tttttatatt cacgatgtgc cgttacctgt ctttttacgt tatgggaaag
     3061 gaatggtgac tttaaattca accagtgggc tttctgcatt gctccataat atgccggtca
     3121 aaaccttagg tagagccaat tatgattttg agggattaac cgataaacag ccattagata
     3181 ctttttgggc taatcctcag ccacctaatt cggaagtatt taaaggctac agacaatttc
     3241 atttacataa aacgcatatt aacggtaatt tttaccataa atcgattttg cgttatcctt
     3301 ataattggga aaatgaagca cac
//
LOCUS       DANLUA010000217         3323 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLUA010000217 DANLUA010000000
VERSION     DANLUA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            11..904
                     /locus_tag="PXB44_002384"
     CDS             11..904
                     /locus_tag="PXB44_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL5282813.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            980..1663
                     /gene="yjjG"
                     /locus_tag="PXB44_002385"
     CDS             980..1663
                     /gene="yjjG"
                     /locus_tag="PXB44_002385"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL5282814.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            1660..2643
                     /gene="cysB"
                     /locus_tag="PXB44_002386"
     CDS             1660..2643
                     /gene="cysB"
                     /locus_tag="PXB44_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL5282815.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            2644..3288
                     /locus_tag="PXB44_002387"
     CDS             2644..3288
                     /locus_tag="PXB44_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282816.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
BASE COUNT         1080 a          570 c          691 g          982 t
ORIGIN      
        1 ttgaaaaatt atgaacaaaa aacttccacc actcaatgca ttgaaagcat ttgaatctgc
       61 tgcacgccat ttaaatttta ctaaagcggc agaagatctc ttcgtcacac aagctgcagt
      121 gagccaccaa attaaactat tagaagattt tttaggtgct tccctgttcc atcgccgtaa
      181 ccgtttgctg gaattaactg agttaggcgc acaatatttc tatgaagttc agccattatt
      241 agaacaaatt gctctcgcaa cagataaaat taggcagcaa agtagtcgtc aaattctgac
      301 tattagcgta ccacaaacat ttggtatgca ttggttagtc ccacgtttgg cggatttcca
      361 cgaaaaatat ccagaagtgg aagtacgtat taagggtgtt gatcaagatg agggattatt
      421 aggtaaagaa acagatgttg ctatttatta tggtaagggg gattggcaag gtctggaatc
      481 tatccgtttg acggaatccc gtattgttat tttagcttcg cctgagtatt tatcgcaaaa
      541 taccattcat ttaccggcgg atttagcagg taagcattta ttacattcct ccacccataa
      601 taagtggaaa agaatggttg aacatttgca tatagaagat atagtagatg cggaaactgg
      661 ctcaattttt agccatactt ttatggtgct tcaagctgca atgcaccaac aaggcattgc
      721 gattgccaac cagttgttgg cacagcacga aattgaaaaa ggtactttaa tagagccttt
      781 cccaaccaca ttgtttgatg aaaaatcgtt ctatgttgtc tatccttcgc aatcagccca
      841 agttcagaaa gtacaatttt ttgttgagtg gattcagcaa aaaatgaaag agtgttgtgc
      901 ttaagcgtgg ctttatcagc ggtatgcgaa aaattagaaa aatttgaccg cttgttatct
      961 aagttaaaaa aggaatatta tgaaatatca atgggtatta tttgatgctg atgaaacctt
     1021 gttttcattt aattcttatc ttggtttaaa agcgatgctg gctcgttacc aaattgattt
     1081 tagtgaacaa gattatcaag actttcaagc ggtaaataaa ccactttggg tggcttacca
     1141 aaataatgag atcactgctc aagatattca aaccagacgt tttgtaaaat tatctgctca
     1201 aacaggcgta gatccgctac aacttaatca agagttaatg gcggagatgg cattcgtcag
     1261 tcagccttta gaaggtgtaa tggatatgtt aaatgcttta tatggtaaag taaaaatggg
     1321 cattatcacc aatggtttta ccgatttgca gcaaaaacgt ttagataata ctcaaacttc
     1381 acattttttt gatattgtag tgatatcaga acaaattggt gccgcaaagc ctgatcgcca
     1441 agtgtttgat tacgcttttg cattaatgga cgaatttgat cgtactaaag tgcttatggt
     1501 aggcgatacc ctcgcttctg atattttagg aggtaataat gctggaattg atacttgttg
     1561 gttaaatttg cttagtaaag agaatgatac ggatattaag ccaacttacg aagtgcatac
     1621 gatgcaacag attattgcga ttgtagaggg gaaatcatta tgaagctaca acagttacgt
     1681 tatatcgttg aaatagtaaa ccagaacttg aatattaccg aggccgcaga ggtgctttac
     1741 acttctcaac cggggattag caaacaagtt cgtttgttgg aaagtgagtt gggaattaat
     1801 atttttgagc gtaacggtaa gcacgttaag gggctaacgc ctgctggtga aaaaattgtg
     1861 gcgattgctc gtgaattatt ggtaaaagcc cagagcatta aatcggttgc tgaagaacaa
     1921 actcgaccca acaaaggcat cttacgcatt gcgaccacca atacccaagc ccgttatatg
     1981 ctaccgagtg taattgagaa gtttaaggct cgctatcctg aagtaagttt gcatttgcac
     2041 caaggctccc cgaatcaaat ttatgatgct ttggtggctg gagaagtgga tttagccatt
     2101 accacagaag ctcaatattt atttgatgaa gcggttttat tgccgtgtta ttggtggaat
     2161 cgttcagtta ttgtcaaacc agagcatcct cttgcggctt ttgtgaacca aagtaaaaat
     2221 ttgactgtcg aacaattagg tagatatgat ttgataacct atacttttgg ttttacaggg
     2281 caatcggatt tagatcatgc cttcaataag gaaggtattt tgcctaatat cgtatttacg
     2341 gcgacagatg ctgatgtaat caaaacttat gtacgcttag gcttaggggt tggtattatg
     2401 gcatcaatgg cacatacgga aacggataaa gacttagtcg ctatcaatgc aagtcacctg
     2461 tttcaaccga gtatgacgca aatcgccttt aaacgaggaa cttttcttcg taactatatg
     2521 tatgatttta ttaattattt ttcgccacat ttaacacggg tacaagtgga gaaagcggaa
     2581 caattacgag ataacaatgc aatttcaagg ttgttcgaaa gtatatcttt agaggagaaa
     2641 tagatgagaa tttggcagaa gttagcgagc gtaatcgtgg tcactttggg cttgatttca
     2701 aacgccagtg cggagaattt taagttgaaa tacagctcaa attatttaat gccagcctac
     2761 attaacttta ataacggtgg tggccaatat aatattcagg caaaaattaa tgtgccttta
     2821 tataatattg tcttttctgc gaagggaaca gaaaaaaacg aacaatttca tatgttgagc
     2881 taccgagata cccgtaacgg caaaacttat gcgatggcag aaattgattc taaaacggta
     2941 aaatatggca aagtgaaaga gccactaaaa gaagaagcgc tgactatgcc aacttatgat
     3001 ttgttcacca cagcctttca gttgagctat tacgataaat taccgagcag ttttcaaact
     3061 actaacggta aaaaacttta cccaacaccg aatgtgcagc tacgcaaagc ccaaaaagag
     3121 gtaaaagtag gcggcaaaac ctaccaagaa attacctaca agtttagaac cagtgataaa
     3181 aaagacatta cggtgaaaaa atttgaaggt gaaaaattcc ctcgctatat cgcctatagt
     3241 cgtgatggcg acaattatga gctaactttt gatagctttg taaaataata aagattagta
     3301 atgagacaag cggtctattt tgt
//
LOCUS       DANLUA010000218         3303 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLUA010000218 DANLUA010000000
VERSION     DANLUA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(14..637)
                     /locus_tag="PXB44_002388"
     CDS             complement(14..637)
                     /locus_tag="PXB44_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5282817.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNTESEPNIQK"
     gene            complement(683..1255)
                     /gene="rpoE"
                     /locus_tag="PXB44_002389"
     CDS             complement(683..1255)
                     /gene="rpoE"
                     /locus_tag="PXB44_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL5282818.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1477..3021)
                     /gene="leuA"
                     /locus_tag="PXB44_002390"
     CDS             complement(1477..3021)
                     /gene="leuA"
                     /locus_tag="PXB44_002390"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL5282819.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          894 a          725 c          585 g         1099 t
ORIGIN      
        1 cttattttac ctattatttt tgtatatttg gctctgattc tgtattggaa gaagtttgtg
       61 ctttttgctt ttcttcctca gataaggtta aatttttaac gttattagta cggcgttgta
      121 actcatggtt ttctaataac gcattaatct tacgctgttg aagctctaac tgttcttctg
      181 ttggttgatc ttgaactggc gcattataac ttaccgcttc aactgaattg ctaaatggca
      241 atgtttgtaa tactggttgt tgcgtttgag ctatttcatc actcgcattc attgaattaa
      301 cccctaatac cgcaactaag cacactgaag ctgcaatacc ggcttgcata attggagttc
      361 cccaacgctt gagcttgagc aataagcctt taggtttctc tacatttgct tgagattcaa
      421 tttcttcatt ttccagtaat gcttccattt tagcagagaa atctctccct aaaatgatct
      481 catcaccttg catcacacta cgaatagtgt gataacttgc ccatttttgc tgtaattcag
      541 ggctgttagt taatgtttct acaaatgctg tatcaacatt ttgcccatcc atataagctg
      601 aaaggcgttc attttgttga gtgatgcgtt cttgcataat tgactccaat gatttaaatc
      661 tcttctaaaa taatctttct atctattgca taagtggctt gattttagca tcaatcgctt
      721 cccttgctcg gaaaatacgg gaacgtacag taccaaccgg acattccatc acttctgcga
      781 tttcttcgta acttagccct tccaattctc ttaaacgaat ggcggtttta agctcatcag
      841 gtaaattttc aatcgtatca aacacgactt tctttaattc ctcggataat atcaaattct
      901 ctggcgtatc actttcacga agttgcgcgc ctacatcata actttcagcg tcttcggcaa
      961 gaatatcttc ttttggcggt cttctgccta aagaggttaa atgatttttc gcagtattca
     1021 ctgcaatgcg ataaagccac gtataaaaag cactttcgcc acggaaagac tctaaagaac
     1081 gataggcttt aataaaagat tcttgcacaa tgtccggaat atcatctctt gcaacataac
     1141 gagtcagcaa accggctact ctattttgat aacgtacaac aagtaaatta aatgcttttt
     1201 tatcaccttt ttgtgctttt tcgactaatt cttggtcggt gatctgttcg ctcaactgcg
     1261 gactctccta aatatcaata tgtcattttc cattgcacac tttgttagtg tgtgctttat
     1321 gacaaaagtt caaaaaattt gaaaaattgt gtaatttttt acattcctag ataaaaccta
     1381 cttaataaac taagcaagcg gtcggatttt agcgaaattt tgcaaaaatt tcaaaaaatc
     1441 cgaccgcttg tctatgttat gctttcgctc tataagttat ttcttcgctt ttaaatctgc
     1501 cactttttgt gaacggtaaa tcgcattaat ggcgtgaatt aaagctcggg ctgaagattc
     1561 cacaatatcg gttgccaaac ccacaccgtg gaattttcga ccttcatatt ccactacaat
     1621 atccacttgc cctaatgctt ctgctccttc gcctttcgca gtcagattat agtgcgacat
     1681 ttttagctcc attcccacaa tgttcataat ggcattgtaa gttgcctcaa ctggaccatt
     1741 tcccccttga gatgtagcac tttgacgaac gccgtctaat tccacctgta caaaagccga
     1801 agccggtaaa ccgctgatta gttgggtggt aattacatct aattttaagc ggtcttcatc
     1861 gccttgctgc atatcaatga aggcaagagc ttctaagtca taatcaaaca cttgcccttt
     1921 tttatcagca agttgcaaga aggcctcata aagtttatct aaatcgtaat ccgtttccgt
     1981 ataacccata tcagccatat gccctttcac cgctgctcta cctgaacggg cagttaaatt
     2041 cagcttctct tttttcaagc cgatggtttc cggcgacata atttcataag tatttttatt
     2101 tttcagcata ccgtcttggt gaatacctga agagtgagca aaggcattgc taccgacaat
     2161 cgctttgttc ggctgaatcg gcatattgca aagctggctg accatttggc tgacacggtg
     2221 aatttcttgg gtattaatgc gggtatcaac ccccataaat tcttggcggg ttttaattgc
     2281 cattaccact tcttcaagag cggtgttacc ggcacgttcg ccaataccgt tgatagtaca
     2341 ctcaatctgt ctcgctccgt tttgcaccgc tgtcaatgag ttggcggtag ccatacctaa
     2401 atcgttatgg cagtgaacag agacaatcgc tttatcgata ttcggaacac ggttcatcac
     2461 ttggtggata atattgccgt attcattcgg taaacagaaa ccgactgtat ccggaatatt
     2521 cactgtggtt gcaccggcat taatggcagc ttctacaatg cggcagatgt tatcaattcc
     2581 agtacgccca gcatcttcac aggaaaactc aacatcatca gtataatttc tggcgcgttt
     2641 taccgctgca accgccattt cgactacatc atcaaatgaa cgttttaatt tagcctcaac
     2701 gtgtaatgcc gagctggcaa taaaggtatg gatacggaat gcttctgcta cttttaaggc
     2761 ttcataagct gcatcaatgt cttttgcaac cgctcgggaa agcgccgcca cccggctgtt
     2821 tttgatatga cgagcgatgg tttgtactga ttcaaaatcg cccgctgaag aaactggaaa
     2881 accgacttcc atcacatcaa cccctaaacg ttctaaagca agggcaattt gcaatttttc
     2941 ttttacggtt aaacttgctt ttaatgattg ctcaccatca cgtaaagtcg tatcaaaaat
     3001 aataatgtta ttgttgctca tagaagcctc tttttaggtg tttttatttg ataaaaaaag
     3061 cccgcatttt aaggtgcggg caagaatgtg gatagtccat ttactttttc caatcaatct
     3121 ttatcctcgc acaaaatacg ataacagaga taatcggagt aaggtaaatt tttccatgtt
     3181 gtgttgtatg tttatgtttg cattgttatt cagtagttga ggtgtatatt atccttatcc
     3241 aaatcaatgt caatagcatt tttctcttca cgcaaccgtt taccagccca ttaattcaag
     3301 tca
//
LOCUS       DANLUA010000219         3279 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLUA010000219 DANLUA010000000
VERSION     DANLUA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            16..705
                     /locus_tag="PXB44_002391"
     CDS             16..705
                     /locus_tag="PXB44_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL5282820.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
     gene            863..1669
                     /locus_tag="PXB44_002392"
     CDS             863..1669
                     /locus_tag="PXB44_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5282821.1"
                     /translation="MKSIKKLTLATLILVGLTACGSGGSGSSSNNAKSKPQVELKKTE
                     EDKKTEEDKKTEEDKKTEEDKKAEEAKKIAEAKGIGNKEYKDGLNELEEKNETGKNGE
                     EIRTYGYLYNSHYSVVTAKMKQTLQENGRQMEPTVEVKGLKTENLPTEGKATYKGEAF
                     DSHGNNSNSVVGGELIYNVDFSSRTGSGLVKNVQGGSIELAQGEIKNDSIIASAHQKY
                     NDQAVGNGSYNIQFFGPNAEEIGGKIELNGEGEGSMKQMLGIAGTREEQK"
     gene            1718..3112
                     /locus_tag="PXB44_002393"
     CDS             1718..3112
                     /locus_tag="PXB44_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5282822.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT         1074 a          535 c          723 g          947 t
ORIGIN      
        1 acaaaggaga cattaatgca aaataataaa accacctacc taaattggta cgatttatgc
       61 gtgctaaccc tgatcctgtt tggtgaggca atttatacct caacccagca atatttcatc
      121 accaccgaaa tgcaaggtaa ttttgaagaa aatttaacct tttcaaatga acaaaattat
      181 tgggcattac tgacgcaact tgcattgttg ctgattgcaa tgggctatct aaaatggcgg
      241 aattttgatt tcagccgttg ggtgattcgt ccctcttggc tggctttttt tagcggtatc
      301 gggttgttta tcttgctctc gttagcgatg gatctctatt ttgaaacatt atatggtgtt
      361 tacaacctag tgtatccgga gccattttgg gcagtattga gagaagtgaa tatttctacc
      421 gtgctgtatg cgattttaaa cggcttttat gaagaaatct ttttcctcgg tatttgcttg
      481 gcggttaaac ctgaatattt aaaatgggcg gtgatttatt cactgattat tcgggtgagt
      541 tttcatacct atcaaggaat ggaaagtgcg ttaggattag gaatttggct cggtttaatt
      601 atttatttgc tctactcacg ctcaaagcag aaaaacctat ttccattttg ggtggcacac
      661 agtattgcgg atattttggg gctgagtttg ttggcttatt tttagttaaa ggtgctacat
      721 ttaagcggca taagttgata gagctgatcg taccagtagc ataaaaacat tattatttgt
      781 atggaaaggt gcttttaggt tacaatataa ccgatagtta ttggttataa ctaaaaatta
      841 tttttaattt aacggagcaa atatgaagag tatcaaaaaa ttaaccctag ccactttgat
      901 tttagtgggt ttaactgctt gtggtagcgg tggttcaggt agcagttcta ataatgctaa
      961 atcgaaacca caagttgagt taaaaaaaac tgaagaggat aaaaaaactg aagaggataa
     1021 aaaaactgaa gaggataaaa aaactgaaga ggataaaaag gctgaagaag caaaaaaaat
     1081 tgcagaggca aaaggaattg gcaataaaga atataaagat ggattaaatg agttagaaga
     1141 aaaaaatgaa acaggtaaaa acggagaaga gattcgaacc tatggttatc tctataacag
     1201 ccattattcc gttgtaactg ccaaaatgaa acaaacttta caggaaaatg gtcgtcaaat
     1261 ggaacctacg gttgaagtga aagggcttaa aactgagaat ttaccgacag agggcaaagc
     1321 aacatataaa ggagaagcct ttgattctca cggtaataac agcaattcag tagtaggtgg
     1381 agaattaatc tataatgttg atttctctag tcgtaccgga tcaggtctag ttaaaaatgt
     1441 acagggcggt tctatcgagt tagcacaagg cgaaataaaa aatgatagta ttatagcttc
     1501 tgctcaccaa aagtataatg atcaggctgt tggaaatggt agttacaata tccaattctt
     1561 tggtccaaat gcagaagaaa ttggagggaa aattgagctt aatggtgagg gcgaaggctc
     1621 aatgaagcag atgttaggta ttgcaggaac ccgagaagaa caaaaataac atttaatacc
     1681 caatgccgtt taatcttaaa cggcattctt ttttactatg tataaaaaac attttctatt
     1741 tttacttgtt tcgcctttag taatggcgga aacggttgag tttcagcaag atcccaaaga
     1801 gtttgatggg cagcgacaag cggttaaatt tgagcaagaa attgcaaaaa aggatttaaa
     1861 attaaccgct tacttggagg aagataattc tcttaaaggt attgaacagc acattaacca
     1921 agcgattctt agacaagatt gggcgaagct ggaaaggctg cttggcgatt atgcaaagca
     1981 agagcaattt gatccgctac ttttagacta tgctttggcg gcattggctt atgctaaaaa
     2041 agattatcgc tctgcggcgt tttattatca gcagttgctg aaaaaacagc ctgatttaac
     2101 ctaccctcgt tttgatttgg ctctgatttt aaaagaggat tatcgctaca gggaagcaga
     2161 gcaagaattt aagcaactca aaaccgatga tttacgcaat gcggtgaaca gtgcgttggc
     2221 tcaaatagag gaggaagaga gctggaagcc ggatttttat ttgcaatata ctcaaaccaa
     2281 caatgtgaat aatgcttctt cgtcgccaac ggtaaatgta aacgggcgga tttttattaa
     2341 agaatcagac agcttgccgc agtcggcaaa aggcgtaaga tatggcgttg gcttgagtaa
     2401 aatgcgaaat atcggcggta atcattttgt gggagtcgat cttggctata acggcgttta
     2461 ttactggaat aaccacgatt atagcgaaca gtcggtctca ctaacaccaa aatatagcta
     2521 cagaacggca acttatcgta tcggggtttc gccctttatt gagcagaatt ggctgggtag
     2581 ctcacggtat aactatcaat ttggtagcag tatcaatgct gttaaacttt ttgaacgagg
     2641 gctgatttcc ggctcattca gccatctgca aaaacgttat agtgaacagc atattgccga
     2701 ccgttacaat ggttatcgca accaactcac gctaggtacg caatggcaag cggtcaaaaa
     2761 ttggcgattt tttgcaaatc ttaacgctag ccgtgaaatt acccgtgaga aagctcaatc
     2821 ttccaataag tggctggcga ccattggcgt aatttaccaa accccaagct gggcaagttc
     2881 ggtaagtgtc ggatatggca agcgttattt tgctgataaa cattatctgt ttggctataa
     2941 acgccaagat aaggaatatc aagcgaatgt ggcggtgtgg aatcgaagtt ggcactggca
     3001 agggattgtg ccgaaattga atttccgtta tcaaaaaatc gatagcaata ttgccgattt
     3061 ctactctcgt agcaataaag aagtctttat gaccttagag aaactatttt aaggctgtgt
     3121 atgaatgtcg gggagaaaaa ttttctcccc gatatttatg tgtagaagat cagacttgat
     3181 ctgacaattc actctaaaaa gtgagagttt cccgtttaga atatgagtgt caaaaatcaa
     3241 tctaaacaaa aaggaaactc tcatgtttta ttctaacaa
//
LOCUS       DANLUA010000220         3263 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLUA010000220 DANLUA010000000
VERSION     DANLUA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(77..781)
                     /locus_tag="PXB44_002394"
     CDS             complement(77..781)
                     /locus_tag="PXB44_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDL5282823.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(872..1699)
                     /locus_tag="PXB44_002395"
     CDS             complement(872..1699)
                     /locus_tag="PXB44_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL5282824.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
     gene            complement(1776..2567)
                     /locus_tag="PXB44_002396"
     CDS             complement(1776..2567)
                     /locus_tag="PXB44_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL5282825.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2625..3158)
                     /gene="aadB"
                     /locus_tag="PXB44_002397"
     CDS             complement(2625..3158)
                     /gene="aadB"
                     /locus_tag="PXB44_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL5282826.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
BASE COUNT          754 a          917 c          797 g          795 t
ORIGIN      
        1 agtgccgatt gaccgagctt taacggcact gttgcaaagt tagcgatgag gcagcctttt
       61 gtcttattca aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg
      121 gatcaccata ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac
      181 ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ccgattatcc
      241 gtttcagttt gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt
      301 caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg
      361 gcgctttatc cgtgttgatg aatcgtggga tctgccactt cttcacgttg ttgaggattt
      421 tacccagaaa ccggtatgca gctttgctgt tacgacggga ggagagataa aaatcgacag
      481 tgcggccccg gctgtcgacg gcccggtaca gatacgccca gcggccattg accttcacgt
      541 aggtttcatc catgtgccac gggcaaagat cggaagggtt acgccagtac cagcgcagcc
      601 gtttttccat ttcaggcgca taacgctgaa cccagcggta aatcgtggag tgatcgacat
      661 tcactccgcg ttcagccagc atctcctgca gctcacggta actgatgccg tatttgcagt
      721 accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca
      781 tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag
      841 tgcccgttga agtaaccggc gctgcgcggt tttatcgcgc agcgtccgag ttgactgccg
      901 ggttgggcgg taacgcttca atagagcgaa ggattgcccg cacgattgcc tccctcttga
      961 aaagatcatc cattctgttt ggcgtatcaa tattcagtgc gaagaatacg gagccagtcg
     1021 gccactcaac ccatcctacc caccaaccca tacggccttc ccagcccgtc tttgcacgca
     1081 gtatccagtt gcgaccggct tccacaatca tgagatcctt gaccaagcgc tgatgttcta
     1141 cccgaaaggg cagctcgtta cgatagagct tcctgagaaa tgcaatttgc tcctgcgccg
     1201 agattgcaag gctgccttct atccagtaat cgccattact tgtcgaagga tcggcgttgc
     1261 catagtcgat tttcttcaaa tagcgccgag ctttgtcatc accaatttcc tttgcaaata
     1321 gctcatacac ccaaacagta gaattccgca ttgctgatcg caaatcttgg tcttgattgt
     1381 ggcctgcaaa gcccctgtta acgccgtccc atcgaaaaat ctggaactca tcacgaacag
     1441 cgcctgcatc aagtgcaaaa agtgtatgag gtatcttgaa tgtcgatgca ggcgagtagc
     1501 gtttcttcga tcgcacagga tcaaaaacca acatggcacg atccgcttgg cgttcgtctg
     1561 ccacaactat cgtgcctttg gcttgaaatt cgctgaaaaa cttcctccag tcagaacgtt
     1621 ctagcgtgcc ttcttgcgca tgcgcgaaag tggcaagaga aaaaatggag aaaagtatcg
     1681 cgaagattcg gattgccatt aacttttcct taatgcccaa cggccaaggt aagccgcgcc
     1741 gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
     1801 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1861 tcttcttgtc caagataagc ctgtctagct tcaagtaaga cgggctgata ctgggcaggt
     1921 aggcgtttta ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttattgcg
     1981 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2041 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2101 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2161 gtcagcaaga tagccagatc aatgtcgatc atggctggct cgaagatacc cgcaagaatg
     2221 tcattgcgct gccattctcc aaattgcagc tcgcgcttag ccggataacg ccacgggatg
     2281 atgtcgtcat gcacgacaag ggtgacttct atagcgcgga gcgtctcgct ctcgccaggg
     2341 aaagccgaag cctccataag atcattgagc aatgctcgcc gcgtcgtttc atcaagcttt
     2401 acggccacag taaccaacaa atcaatatcg ctgtatggct tcaggccgcc atccactgcg
     2461 gagccgtaca aatgcacggc cagcaacgtt gattccagat ggcgctcaat gacgcttagc
     2521 acctctgata gttggttcga aatttcgatg gtcaccgctt ccctcatgat gtctaacacc
     2581 tgagttaagc cgcgccgcga agcggcgtcg gcttggacga attgttaggc cgcatatcgc
     2641 gacctgaaag cggcacgcaa gacctcaacc ttttccgccc cgagtgaggt gcatgcgagc
     2701 ctgtaggact ctatgtgctt tgtaggccag tccactggtg gtacttcatc ggcatagtaa
     2761 aagtaatccc agatgatcgc ctcccagctg ttacaacgga ctggccgccc ggcgatgacg
     2821 ccctcagccg cctctgggca cgagccctgc ggagcctccg cgatttcata cgcttcgtct
     2881 gcccaccaag caggttcgca gtcaagtaac tcatccccga tctccgctaa gaatccatag
     2941 tccaactcct ccatgacgcg cccgccgagc atttcaacta ttgcctcgag ctcgccgcgc
     3001 ctctcgccgg gaaacgtcag atcaatatca tcgtgcttgc gtgttacacg ccctagccgt
     3061 gcatcgatcg cccagccccc accgatccag agcggcagat ttcgctcatc tgccgcagct
     3121 agaattttgt gtatcaatgt gacctgcgtt gtgtccatgc ggcctaacct ttgttttagg
     3181 gcgactgccc tgctgcgtaa catcgttgct gctaatacgg tgatcagcag tataataata
     3241 attgatttat tcattgagat cct
//
LOCUS       DANLUA010000221         3248 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLUA010000221 DANLUA010000000
VERSION     DANLUA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            209..586
                     /locus_tag="PXB44_002398"
     CDS             209..586
                     /locus_tag="PXB44_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5282827.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            1316..2092
                     /locus_tag="PXB44_002399"
     CDS             1316..2092
                     /locus_tag="PXB44_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5282828.1"
                     /translation="MNYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVN
                     TALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYNTHLKNYIYNVQHTAVGSAPSL
                     FIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVSFTDASSRVDAASHYG
                     FKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGKSKRIAIDEFNYTYYP
                     GTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
     gene            2696..3190
                     /locus_tag="PXB44_002400"
     CDS             2696..3190
                     /locus_tag="PXB44_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5282829.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
BASE COUNT         1097 a          530 c          608 g         1013 t
ORIGIN      
        1 tgacttatac aacacttatc tgccccttac ctagggtatt cgcagaggaa ggttatatgt
       61 tagatacggt tgaggttaaa gcccaagcaa taaaaaaaca gatgaagttt tcattaaaac
      121 aggggcaata agcacccgag ataaaatctt ggaatcaaac caaagcttag agactattgt
      181 tcgttctatg cctggcgcat ttacacaaat ggataaatct caaggtgtgg tttctgttaa
      241 tattcgagga ggttctggat ttgggcgagc taataatgca gtaggcccaa attatatggg
      301 aatgctcgct ggcaaatacc aattcaatga ctctacttgg ataggagcat tatttgggca
      361 tagttggcgc aaaatctctc aaaattataa aataggaggc ggacgaaaag ttacggatag
      421 ctcgattgac cttagtaaaa ttgatgatct agataaagaa agttctacca cctcaccgtt
      481 caatgcagct catttaaaac aaaaaccaat gagtcgtatg gctaaattgg aatatggcga
      541 taaataccaa accatcacac tattaccaaa gtcaggtagc gggtagacat ttaaaaaacc
      601 aaaacaacca gttaaattat cgttttcact tgcccgagtc accttggtta gatgttcaag
      661 tggcctatgc ggaaaatcaa ggcaaacaaa actacaacaa agggagctga attaataata
      721 aatatttaat tcaaggtttg gcaattaaca acaaagcgac aaccttaaat ttgagtaaca
      781 cctttaaagt gtctccgttt aaagatggag aatgggaagc aatattaggt tttaatcgtt
      841 taaagaaccg ctatagtaaa aatagacatc ctgcggaatt aaactataac ttagagaatg
      901 gcgaaaccag cgaaaacaac gattctgtag gaatgacagc cgttcgtaaa tcgctttata
      961 cttcaacttt ccagcctgat ggcgaacaga agttcgatac tttttatctc gacaatcact
     1021 ttaactatgg tatatgggga ttggatataa acgcaaatat taataccgct cggtttattg
     1081 gtcagcgttt taaatatttt ccttttgcga ttagagaatt ggagtcagat attagacagg
     1141 caagtagaaa taggcagttt gagcatttaa ggtcattaca atctcaatta gacaacttgc
     1201 aaaaaaacat tgtacatata ttccagatga ggattttcct aatgatcctg aattgggaga
     1261 gtttaactgt cctgatgtca attttcctca tacagcaaca ggaaaacata aacgaattaa
     1321 ttattcagca accttgtctg cttatattca cgatttattc acgcccttta ttagttattc
     1381 tcaaacacat cgagccccta atattaaaga aatattcttc tctaatttag gtgactatgg
     1441 tgtaaatacc gcactacaac cagagcaagc gaaaacaaca cagtttggta ttaatggatt
     1501 caaagaacgg gtatttagtg aagaagataa attaggattt aaagtgctat tttataacac
     1561 tcacttaaaa aactatattt ataatgtaca acataccgct gttggtagtg ctcctagttt
     1621 attcattttc cacaaaaact atgaaaaacc agtaaatatc aaagggatag aattagaatt
     1681 tagttatgat ataggctctt tctatggcaa cttagcttat gctcgtcaaa atacgaatca
     1741 acccgtatcc tttacagatg caagttcaag ggtagatgcc gcttctcatt atggttttaa
     1801 aactcaaggt tttggtgcca gcaaaatttc agcgttacca agagactatg gctcattgga
     1861 gttagggaca agatggtttg atcaaagact acaaattggc ggagtggcaa aatattacgg
     1921 aaaaagtaaa cgtattgcga tagatgagtt taactatact tattatcctg gaacacatat
     1981 tgtgaaagaa actcatcggc ttgaagaaac cgtaaataaa caacctatta ttgttgattt
     2041 ttacgtgagc tacgaaccaa tcaagtcgct tatattaaag ttagaactat aaaatgtctt
     2101 tgacaaaaag tacattgatc ctcttgatgc taataatgat gcagcaagtc agagggttta
     2161 caattctgat atcagtgatg aacctattta tacgctcaat aattatgcaa gagggagaac
     2221 tatagtgttt agcacaagtt ataaattcta aatggggttg tcctagataa gctagcttaa
     2281 atttccttta actaattatt tggcatagtg actaaaattg agtctgcaca gtaggttaaa
     2341 accttataat ataaggattt aacccagatg gtgcaagcgt ccacgcttgt gccagttaat
     2401 tcctgttctg aaagtatttc agtcataaga taaaagccta aatcagccaa gtaaaatttg
     2461 gtttatttgg gcttttgttt ttggcacaag cgtggacgct tgcgtcatca ttttatttat
     2521 ccatccattg ctatcatttc agtatttctt tggcttatca caagcggtta aattttatca
     2581 acactttgca agttttaatg atttttgcga tctagatcga aaaagttata ttggcttgtg
     2641 gaatttaatt ttgaataggg tatttttctt attccttttt aagttgagaa aggctatgag
     2701 ccgaaaatat aaattccacg aaaagagcgg tgcttatttt gtcagttttg caactgttta
     2761 ttgggttggt gtgtttgtga gagagattta ttttcaggta attgttgatg ccttagatta
     2821 ctgcagaaaa cataagggaa tgattttgta tgcctattgt attatgccaa ctcatatcca
     2881 tttattattt caagcagaaa atcaaaatcc ttctgattta atacgagatt tcaaaacatt
     2941 caccgctaaa atacttattc agagaattaa agataatttg caagaaaacc gtcgggaatg
     3001 gttgctgtgg atgttttctc gggcggctga acgacgttgt aatgtaaaac aatatcaatt
     3061 ctggcaacat cataaccaac ctattgaaat ctattcgcag aaattctttg atgaaaagtt
     3121 aaattatatt catcaaaatc cagtggtaag tggttttgtg tctgagcctt gggaatggaa
     3181 atataattga aataaactgc ttgtattttt gcgatctctg tcgaaaaatt gtgaattttt
     3241 acttgtct
//
LOCUS       DANLUA010000222         3188 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLUA010000222 DANLUA010000000
VERSION     DANLUA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            74..526
                     /gene="iscR"
                     /locus_tag="PXB44_002401"
     CDS             74..526
                     /gene="iscR"
                     /locus_tag="PXB44_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL5282830.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            581..1801
                     /locus_tag="PXB44_002402"
     CDS             581..1801
                     /locus_tag="PXB44_002402"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL5282831.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            1928..2311
                     /gene="iscU"
                     /locus_tag="PXB44_002403"
     CDS             1928..2311
                     /gene="iscU"
                     /locus_tag="PXB44_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL5282832.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            2420..2743
                     /gene="iscA"
                     /locus_tag="PXB44_002404"
     CDS             2420..2743
                     /gene="iscA"
                     /locus_tag="PXB44_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL5282833.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            2753..>3188
                     /gene="hscB"
                     /locus_tag="PXB44_002405"
     CDS             2753..>3188
                     /gene="hscB"
                     /locus_tag="PXB44_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL5282834.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQK"
BASE COUNT         1040 a          566 c          708 g          874 t
ORIGIN      
        1 ctaaattggt ggggtatatc aaaatttagc aactgtttca gaagattttc tggataatca
       61 atgaggatat tttatgaagc ttacctcaaa agggcgttat gcagtaaccg ccattttaga
      121 tattgcttta cacggaaaac tagagccggt tagcttagcc gatatttcag atcgccaagc
      181 gatttcactg tcttatttag agcaactgtt tgctcaactt cgccgtgacg gtatcgtgca
      241 gagcgttcgt ggaccgggtg gtgggtatca attaggtaaa aaaccggaag agattagcgt
      301 gggtatgatc atctccgccg tgaatgaaaa tattgatgtc accaaatgta aaggtagtgg
      361 taattgccgt aaagattctc agtgtttaac gcattctttg tgggagcggt tagaagatca
      421 aattgagcag tttttaggga caatttcagt tgcggattta gccgcagaac acgctgatca
      481 cgattgtgaa aaagagcatt gtcacgatca tcctcatcat cactaattgc aaatttttta
      541 tcaaaaatga ccgcttgggt tgttttaact aggagtaaca atgaaattac caatttattt
      601 ggattacgca gcaaccacac caatggacga acgtgttgcg aaaaaaatga tggaatatat
      661 gaccaaagac ggtatctttg gtaaccctgc ttctcgctca cacaaatttg gttgggaagc
      721 ggaagaagcg gttgatgtgg ctcgtaacca aattgccgat ttaatcggtg cagacagccg
      781 tgaaatcgtt ttcacttctg gtgcaacaga gtcggataac ttagccatca aaggtgcggc
      841 tcatttctat caaaccaaag gtaaacacat tatcactgtt aaaaccgaac ataaagcagt
      901 attagatact tgccgtcagt tagagcgtga aggttttgaa gtgacttact tagaccctga
      961 agaagacggt attttagatt tagctaaatt aaaagcggct attcgtccgg acacaatttt
     1021 agtttcggta atgcacgtaa ataacgaaat gggcgtaatc caaccgatta aagagatcgg
     1081 tgcaatctgc cgtgcgaaga aaatcatttt ccacgttgat gcgacccagt ctgttggtaa
     1141 aatcgaagtg aatgtacaag acttaaatgt tgatttaatg tctttctcaa gccacaaatt
     1201 atacggacca aaaggtatcg gcggtctata tgtttgtcgt aaaccacgtg tgcgtttaga
     1261 agcaattatt cacggtggcg gtcacgagcg tggtatgcgt tcgggtacat tgcctgttca
     1321 ccaaattgta ggtatgggtg aagcatatcg tatcgctaaa gaagaaatgg caacggaaat
     1381 ggctcgttta accgtgttac gtgatcgttt atataacggc tttaaagata ttgaagaagt
     1441 atatgtaaac ggttcaatgg aagaaggcaa acgtatcggt tcaaacctaa acatcagctt
     1501 taactttgtt gagggcgaat cgttaatgat gtcattacgt gatatcgcag tatcatcagg
     1561 ttcagcttgt acttctgcaa gtttagagcc atcttatgtg ttacgtgcaa tcggtcgtga
     1621 cgatgaatta gctcacagct caatccgttt cactttaggt cgttggacga ctgaagaaga
     1681 aatcgatcat actattgaaa tcgtgaaaaa agctatcgtg aaattgcgtg agctttcgcc
     1741 tctttgggaa atgttcaaag aaggtgtaga tttgtctaaa atcgaatgga atcaccacta
     1801 attcagaaaa attttgcaag ggcaaataat atttgcccct acaaaataag ctttcatcac
     1861 aaatagtaaa ttgaatgtag gggcgaaata ggtttcgccg gaaataggaa ataaaaggaa
     1921 tacaaaaatg gcatatagcg aaaaagtaat tgatcattac gaaaacccac gcaacgtagg
     1981 gacttttgat aaagaagcat cagacaccgg tacaggtatg gtgggagcac cggcttgtgg
     2041 tgatatttta cgtttacaaa ttaaagtgaa cgatcaaggt atcattgaag atgcacgctt
     2101 taaagcatac ggttgtggtt ctgcaatcgc atcaagttct ctcattaccg aatgggtaaa
     2161 aggtaaatca ttagaggaag caggtgcgat taaaaacagt gatatcgctg aagagttaga
     2221 attaccaccg gtaaaagttc actgttcaat cttagcagaa gatgcaatca aagctgcaat
     2281 tgcagactac aaagagaaac aagctaagta atatgataca atcaatgcaa gcggtcgatt
     2341 tttgcaaaaa atttgcaaaa tttaaccgct tgcatttgct atttctatct tgatttaatc
     2401 cattcacata aggaaaacta tgagcattac cttaacagaa gctgccgcaa accgagttcg
     2461 tacattctta gaaaacagag gtaaaggtat cgggctaagg cttggtgtga aaacttccgg
     2521 ttgttcgggg cttgcctatg tgcttgaatt tgttgatgta ttaaatgaag acgatcaggt
     2581 tttcgaacaa cacggcgtaa aagttattgt cgatgaaaaa agcctgactt acttaaacgg
     2641 tacgcagctg gattttgtga aagaaggctt aaacgaaggc tttaaataca ccaacccgaa
     2701 tgttaaaaac gaatgcggct gtggcgaaag ttttagtgtg taaggaactg taatgaataa
     2761 cccttttact ttatttgatt tacccgttca atttcaactt gataacgccc agctttctga
     2821 gcgttatctt gctttacaga aacagcttca cccagataac tttgcccaca aatcaagtgc
     2881 tgaacagtta gaagcggtgc aaaaatctgc tgatgtcaat gaggcactca atattcttaa
     2941 agatccgatt ttacgggcag aagcgatgat tgaaatccat acaggtgaag ccaaaaatct
     3001 cgaagaaaaa agtatgcgag atgtggattt cttaatgcag caactcgaat ggcacgaaaa
     3061 actggaaacc attgagcatc gtaaagacga aaccgaactg accgtctttt taaaacagat
     3121 taaagcggaa caaaaagcag tattagaaca gcttgaacaa acgttaaata cagccgactg
     3181 gcaaaaag
//
LOCUS       DANLUA010000223         3143 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLUA010000223 DANLUA010000000
VERSION     DANLUA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            19..1113
                     /locus_tag="PXB44_002406"
     CDS             19..1113
                     /locus_tag="PXB44_002406"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5282835.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
     gene            1159..2457
                     /gene="aroA"
                     /locus_tag="PXB44_002407"
     CDS             1159..2457
                     /gene="aroA"
                     /locus_tag="PXB44_002407"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL5282836.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(2511..3122)
                     /locus_tag="PXB44_002408"
     CDS             complement(2511..3122)
                     /locus_tag="PXB44_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL5282837.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
BASE COUNT          927 a          622 c          695 g          899 t
ORIGIN      
        1 gaataatcag aggttattat gcaatttatc aacatcgcaa atgagggcgt gaaatccctt
       61 tcgccttacc aagcaggaaa gccgattgag gagctagagc gtgaattagg gattcaaaat
      121 atcattaaat tagcctcgaa cgaaaaccca tttggttttc ctgaaagtgc aaaaaaggcg
      181 attctgaatc agcttgacga tttaacccgc tatccggatt caaacggctt tgaattaaaa
      241 accactattg cccaaaaatt tggcgtacaa gccaatcaaa tcaccttagg caatggctca
      301 aacgatttat tagagctgtt tgcccacacc ttcgccggtg agcaagatga aattatctat
      361 tctcagtatg cgtttattgt gtatccgttg gtgacgaaag cgattaatgc cgttgcccgt
      421 gaaattctgg ctaaaaattg gggacacaat ttagatgcct ttttggctgc aattaccgaa
      481 aaaactaaat taatttttat tgccaacccg aataatccga ccggtaattt tttaacccaa
      541 gcggaaattg agggcttttt agctaaagtg ccggcaaatg taatcgtggt gctggacgaa
      601 gcttataccg agtttactgc gcctcaagaa cgggtggatt cgtttggctt attggcaaaa
      661 tacccgaatt taattgtgtc acgttcactc tctaaggctt acggcttagc cgggcttcgg
      721 attggttatg cggtttcaaa ccctgaaatt gcggatttat taaatcgtgt tcgccagccg
      781 tttaactgca atagtcttgc cttagcttca gcgattgcgg taatgaatga tgatgaattt
      841 gtcaaaaaag tggcagaaaa taaccgcttg gaaatggctc gctatgaggc tttctgccaa
      901 gcaaacggct tggaatatat cccttctaag ggcaatttta ttactattga tttcaaacgt
      961 cctgccgctc cgatttatga ggcattactg cgtgaaggcg tgattgttcg ctcgatagca
     1021 ggttatggaa tgccgaatca tttacgcatt agtatcggtt taccgcaaga aaacgagaga
     1081 ttttttactg ccttattgaa agtgttagct taacaagcgg ttacctttta tgaaaatttt
     1141 acaaatttaa agagaaaaat ggaaaaacta actttaaccc cgatttcccg agtagaaggc
     1201 gagatcaatt tacctggttc taaaagcctg tctaaccgag ccttattatt agccgcctta
     1261 gccaccggta cgactcaagt gaccaattta ttagatagtg atgatattcg acatatgctc
     1321 aatgccttaa aagcgttagg cgtgaaatat gagctatcgg acgataaaac cgtctgtgta
     1381 gttgaaggga ttggtggagc ttttaaggtt caaaacggct tatcactgtt tctcggcaat
     1441 gcaggcacgg caatgcgacc acttgcagca gcattgtgtt taaaaggtga ggaaaaatcc
     1501 caaatcattc ttaccggtga accaagaatg aaagaacgcc cgattaaaca cttagtcgat
     1561 gctttacgcc aagtaggggc agaggtacag tatttagaaa atgaaggcta tccaccgttg
     1621 gcaattagca atagcggttt gcagggcgga aaagtgcaaa ttgacggctc gatttccagc
     1681 caatttctaa ccgcattgct gatgtctgcc ccattagcgg aaggcgatat ggaaattgag
     1741 attatcggtg atctggtatc aaaaccttat attgatatta ccctttcgat gatgaacgat
     1801 tttggtatta cggttgaaaa tcgagattac aaaacctttt tagttaaagg taaacaaggc
     1861 tatgttgctc cacaaggtaa ttatttggtg gagggagatg cctcttctgc ctcttatttc
     1921 ttagcctccg gtgcgattaa aggcaaggta aaagtaacgg gcattggtaa aaaatcgatc
     1981 caaggcgacc gcttgtttgc cgatgtgttg gaaaaaatgg gggcaaaaat cacttgggga
     2041 gaggatttta ttcaagccga gcaatccccg ctaaaaggcg tagatatgga tatgaatcat
     2101 attcctgatg cggcaatgac gattgcaaca accgctttat ttgccgaagg agaaacagtt
     2161 atccgcaata tttataactg gcgggtaaaa gaaaccgacc gcttgacagc aatggcaacc
     2221 gaattgcgta aagtcggggc agaggtagaa gaaggggaag attttattcg gattcaaccg
     2281 cttgcgttag aaaacttcca gcacgctgaa attgaaacct ataacgatca ccgtatggca
     2341 atgtgttttt cattaattgc gttatcgaat acagaagtga cgatcttaga tccaaattgt
     2401 accgctaaaa cgttcccgac ttactttagg gagttggaaa aattatcggt cagataaaag
     2461 taaaaaagga ttcagaaaac tgaatccttt ttacgtttta ttgtggcaga ctaagcccaa
     2521 ccgctgttgt tacgtctgcg gcgagggatc aggaaaggca cgagtaaccc aagtagtaaa
     2581 cctgcgccta acacagcacc accatagatg aaccactgaa tcacgatttc acgtttctcg
     2641 gaatcgtgca ttgtttctaa atcacgattt ttgtttttca ggatttccag ttcacgtttt
     2701 agttgtgcat tttcctcaac caaatcactg cttttctgca ctgaatcttg cgttctacgt
     2761 tgcatttctg cggtacgttg ttgccattcg gtatcgattt tgctcaggcg attggttaaa
     2821 tcttgaacct gttgttttaa ttgaggaatc agctcttttg gactagcagt atcgcttaag
     2881 tctgaattaa gtacccaacc ttcacggtta cgactatctc gaatcagact gtagcgctct
     2941 ttacgatcta atactgtgac tttttcacct gcttgaatag cgcctgaaat gcggaattga
     3001 tcaccagcac ctttgcgcat aaatgtactc agattttcag taatgtaagt gtctgccgtt
     3061 aaagcagggc tagataggcc gagtaaaaaa caagacagaa gagctgatga taatttcttc
     3121 atatatattc ctctaaagct agg
//
LOCUS       DANLUA010000224         3098 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLUA010000224 DANLUA010000000
VERSION     DANLUA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..543
                     /locus_tag="PXB44_002409"
     CDS             <1..543
                     /locus_tag="PXB44_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5282838.1"
                     /translation="LETTANKIKRSKDDSIEWHRKNEKEIPFWVITETWDFGALSKFY
                     SLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHHSRIWNRKFSDIAIPKLDDP
                     RWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQTSTNYHWLDKFEDLLNDFPN
                     LPNTKVELMGFNREILEKIK"
     gene            complement(607..1569)
                     /gene="hemH"
                     /locus_tag="PXB44_002410"
     CDS             complement(607..1569)
                     /gene="hemH"
                     /locus_tag="PXB44_002410"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL5282839.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
     gene            1806..1940
                     /gene="rpmH"
                     /locus_tag="PXB44_002411"
     CDS             1806..1940
                     /gene="rpmH"
                     /locus_tag="PXB44_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL5282840.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            1976..2350
                     /gene="rnpA"
                     /locus_tag="PXB44_002412"
     CDS             1976..2350
                     /gene="rnpA"
                     /locus_tag="PXB44_002412"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL5282841.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            2299..2622
                     /gene="yidD"
                     /locus_tag="PXB44_002413"
     CDS             2299..2622
                     /gene="yidD"
                     /locus_tag="PXB44_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL5282842.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(2727..3068)
                     /gene="erpA"
                     /locus_tag="PXB44_002414"
     CDS             complement(2727..3068)
                     /gene="erpA"
                     /locus_tag="PXB44_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL5282843.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
BASE COUNT          940 a          624 c          595 g          939 t
ORIGIN      
        1 ctagaaacta cagctaataa aattaagcgt agtaaagatg attctattga atggcataga
       61 aaaaatgaga aggaaatccc tttttgggta attactgaaa cctgggattt tggtgcatta
      121 tctaagtttt actcattgtt aaatgggcaa caccaaagga aaattgctaa gaaatttaat
      181 gtagatgaga atacgttaaa gaattggttg aatgaaataa acattgtaag aaacctttct
      241 gctcaccatt ctcgtatttg gaacagaaaa tttagcgata ttgctattcc taagctagat
      301 gatcctcgtt ggtcgaaatt taaaaaaatg cagcagtatt tttcaaactt atcgctggat
      361 gacaaagcaa aggagagaat tttcggaagg gtagctattc tgtggttttt agttagtcaa
      421 accagcacta attaccattg gttagataag tttgaagatt tattaaatga cttccctaat
      481 ttaccgaata caaaagtaga actgatggga tttaacagag aaatattaga gaaaattaaa
      541 taaaataagt acattcctat agggtgtaga taaaaatggg cggatttatt atccgcccaa
      601 attatattat attttcgctt cgattaattt tactaatgcc ttgatatgat cgccactcgc
      661 atttaacgct ggaatgtagc gataagattc tccgccggca tgttcaaagt tctcgcggtt
      721 ttcttctgta atttcttcta aggtttccaa acaatctgct gaaaagcccg ggcaaacgac
      781 agccactttt tttatccctt tttgtgggaa gctttcaagg gtttgatcgg tataaggctg
      841 tagccactct tcatcaccaa aacgggactg gaaagacaaa taccattttt ctcttggcag
      901 acctagcttt tcagccacta attctgctgt ttcttggcaa tgcttggcgt aaaaatcacc
      961 ttccgtttca taacgtttag gaatgccgtg aaatgaaaac agtaataact cgtcaggttt
     1021 aacatcaata gagttagcca acgcttgaat ataaagcgga tcgttatgat agctgtgaat
     1081 aaaatcaaac ggcacgattt tacgctgtgc ttttaaaccg ttggcaaacg catcaaacac
     1141 cgaagcggta gtagtgctac tgtattgcgg ataaagcggt agcacaataa tccgctcaac
     1201 cccctgcttg atgaggttat cggtcgcact ttcaatactt ggattgccgt aagacattcc
     1261 caattccacc accacatttt tgcctttggc atcaaaatag cgttgtaccg ctcgttgttg
     1321 ttgccgagaa atcgccaata acggtgagcc ttcttcgctc catactgatt gatataattt
     1381 tgccactttt ggcgaacgtc ttggcaaaat gatgcagttt aggattgctt gccatttaaa
     1441 ttttggtaga tcgatcacac gaggatcgct taaaaattgc tttaaataac gcttcaccgc
     1501 acttggcgtg ggttcatctg gcgtacctaa attagccagc aaaataccta tttgttgggg
     1561 ttgattcatt gttctatttc tcatcaatag ttaatatttt ttcgattatg ccacaaacca
     1621 atataaattt ctgcgattat aacaagcggt cgtatttttc aaaaattttg caaacaaccc
     1681 gagttgtgct tggattgttt cttgcttttt attttaattg cctgtagaat tgcaactctt
     1741 ttttatggtc cttgtttggc agaaaccatt ttatgccaac tttttaaatt ttaggtagat
     1801 aagcaatgaa acgtacattt caaccatcag tattaaaacg tgctcgtact cacggtttcc
     1861 gtgctcgtat ggctactaaa aacggtcgtc aagtgttagc gcgtcgtcgt gctaaaggtc
     1921 gtaaatcttt atctgcataa ttgcagaacc agccaaaaca cctttctgat taagtgtgaa
     1981 gaagctaaat ttctctcggg agctacgttt gttagctccc gttcaattta aggcggtgtt
     2041 tgaagagcca cttcgagcca gcacacccca aatgactctt cttgctagag agaataatct
     2101 agaaaatcct cgtctaggct taactgttgc caaaaaacat ttaaaacggg ctcacgaccg
     2161 taatcgcatt aagcggatag tgcgtgaaag ttttcgcctc aagcaacacg aattgccgaa
     2221 tttcgatttt gtatttgtgg caaaaggcgg cattggcaaa ttagacaacg caacgctgtt
     2281 tgaaacattg gaaaaactat ggtcaagaca cattcgcctg agcaaaaagc cggcacaaca
     2341 gaatccataa atcagccacc tcgtctcagc ctattagcca aaattctatt gctaccgatt
     2401 tatttctatc gctatgctat cagcccattg attggcccac gttgccgatt ttacccaacc
     2461 tgctccacct atgcggtcga agcgattaaa atccacggag caattaaagg tgggtattta
     2521 gcgtttaagc gtattctgcg ttgcaaccct ttgagtgagg gaggcgaaga tcctgtccca
     2581 ctttgttgta aaggcaactg caaacataat gataaaaaat agcctaaact caataagtta
     2641 ggctattttg tttaatacaa acggtcattt ttagtaaaaa atttacttgt gagactgata
     2701 ttgaataagg caataattcg agtgaattaa atgctaaagg aagaaccaca accacaggtt
     2761 gagcttgcat ttggattatt cacgacaaaa cgtgagccgt ccagcccttc ggtataatcc
     2821 accgtgccac caattaaata ttgtaggctc atcgggtcaa ccaccaagcc aacgctttga
     2881 ttctcaatgg taaggtctcc ctcattgact tgatcatcaa aggtgaaccc atactggaat
     2941 ccactacagc ccccacccgt aatatacaca cgcaagcgaa gattcgggtt ctcttcgccc
     3001 tcaattaagc tttttacttt acgagccgcc gcatcagtaa agattaaagg aatttgaata
     3061 tcgctcattt tgttttagat caaaatttta ctgtgatt
//
LOCUS       DANLUA010000225         3075 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLUA010000225 DANLUA010000000
VERSION     DANLUA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            94..315
                     /locus_tag="PXB44_002415"
     CDS             94..315
                     /locus_tag="PXB44_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL5282844.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            320..2053
                     /locus_tag="PXB44_002416"
     CDS             320..2053
                     /locus_tag="PXB44_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL5282845.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(2120..2821)
                     /locus_tag="PXB44_002417"
     CDS             complement(2120..2821)
                     /locus_tag="PXB44_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5282846.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(2818..>3075)
                     /locus_tag="PXB44_002418"
     CDS             complement(2818..>3075)
                     /locus_tag="PXB44_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5282847.1"
                     /translation="RKAQRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEP
                     SSAGIMMTVYAWVVFLLSLPLMLLTANMERKRLLLMLFMGE"
BASE COUNT          964 a          699 c          571 g          841 t
ORIGIN      
        1 caaaaatcac tcaaacaata acgcttaacg ctactttatt attatttttg tgctactatt
       61 tgtacctaat tataggatca tctttatttc aatatggcaa cgaaatctaa ataccacacc
      121 aaacaatttg agcagctatt ggctgacctc atcgccacca tagaaaagca taaagcaccg
      181 gtggatttat cgttaatggc attaggcaat atgataacta acattttaat tgaaaatatt
      241 caaagcccgg cacaaagaga agctctcgct gatgcctttg ccgatgcatt aaaacaatca
      301 ctaaaaacca aataagatga tgtttgcacg cctacgcagt ttattgccga ccaattctcg
      361 ccaatatcga gaagaaacct cacagaaaat cacgtggggg cattggtttg cactatttaa
      421 tattgtgttg gcactactca tttcctctcg ttacgcattt aatgcagact ggccgaacac
      481 gcttgtaggc aaactttatt tctttgtcag cctgttcggg catttcagct ttgtggtctt
      541 tgcaggattt ctattaattc tgtttccgct cagttttatt gttaagaatc atcgcaccta
      601 tcgtgggctt tccgtgattt tagccaccat cgggcaaacc gtgttattgg tggatactga
      661 agtcttcaag caattttatc tgcatttatc accgcttgta tgggatctgt tagttaatcc
      721 ggaagaaggg gaactgaccc gtaaatggca actgctgttt gtaccaatgc cactcctttt
      781 gctggcagaa atgctctact ctcgctggtg ctggcagaaa ctgcgtagtt ttagccgcca
      841 aaaatggggc aaatatgtcg ctctcttttt cgtgatcagc tttaccgcta ctcatttaat
      901 ctatgcgtgg gcagatatga atgcctaccg cccgattacc gctcaacgag caaactaccc
      961 actttcttac ccgatgacgg ctcgtaattt cttagaaaaa cacggtttta ttgaccgagt
     1021 agaatttaat caagaaattg aagaaagtgg cagactagat actttctatc tgaattatcc
     1081 aaaatctccc ttgattgcga ccaaaacgcc ctcaactaat attttactga ttaatttatc
     1141 aggtttatct aaacagataa taaatgccga aacaatgcct aagctgacag aaatcagcca
     1201 acaaagccac cgctttatgc agcattacag tagtggtgat tcagcctctg ccggtgcatt
     1261 aggtctattt tacagtttaa gcggtaaata tttggattcc gttttagccg ataaaaccgt
     1321 gtcaccgctt atcgaaatcg ctagaaaatc ggactatcaa ttagggctgt tttcacataa
     1381 tggttttgcc gacccgattt atcatcaggc gatcttctct ggtattttat tgcctacggc
     1441 gcacagtaat gtaggggcga ttgggcaatg gaaaaactgg atcagctctc gctcattgga
     1501 aaatccgttc tttagctatt tagacttaag aatgcccttc gcccacaccg ctcaagagca
     1561 ggcaaaatta ttagattggc aattcgctga aatctggcaa catttgaccc aaaatgcact
     1621 attggcgaat acgctggtaa tcattaccgc cgatattagt aatgaagttg gagcagaaaa
     1681 tagcttcgat aaacaacaca ttcaagtgcc gatgatgttc tactggcaag gcgaaagcaa
     1741 agagtaccat ttcctttcaa gccatttaga tattatgcca accttactga gccaattctt
     1801 cggcgtgcaa aatccgctga atgattatgc acaaggtatg gatttaagca cccagaacca
     1861 acgtttatgg gtatccagtt ctaatcacaa atggaatgtg gcgattatgc cgaatggcga
     1921 tcaatatcaa attgatcgta aaggcaactt taagcacttt aacagcaatg gcgaacacga
     1981 gaaagatgct cgaccaccat tagcgttgtt cttacaaatg attcaacaaa gcaatcaatt
     2041 tattgaaaag taatgttcaa caagcggtca tttttgcagg aaaatttgca aaatttcagc
     2101 aaaatatgac cgcttgtcac tatctgagct gtctatattt caagctaaca aatacaaacc
     2161 aaaccattgc tacaaaagca atactcccac cggcaaatcc gatattggct aatcccagtt
     2221 gctgaatagc gatgctcccc agtaatgctc caccgccaat gcctaaatta taagtgccgg
     2281 aaaaaattgc agttgcgaca tcagtcgcat caggggcaag ttcaagcacc ttcatttgca
     2341 acgcaatgct cattccggta atgccaattc cccagacaaa agcgaccata aacattgcca
     2401 ctgttgagtc actcaaaaac agtaaacaaa gctggctgca cataataatg gcgatcccgc
     2461 ttaagataaa tttattcggg gctttgccat ataaccgacc aaataaaaaa cttgccacca
     2521 cacctgccaa gccaaaaacc agcaaaataa aggtggtgtt ttccacactc accgaactga
     2581 ttttaatcat aaacggctca atatagctgt aagcggtaaa atgtcctgaa acgatcaatg
     2641 ccaccagcaa ataaatgccg attaacatcg ggcgtttaaa taacaaaggc aaacttttta
     2701 tcgagcctgc atttttgctt ggtaaataag gcagcaaccg ccaaatcatc attagcacaa
     2761 tcaaagcgat aatcccaata atgccaaagg ttgcccgcca gcctaaggct tgcccgatca
     2821 ttcgcccata aataacatca gcaacagccg ttttctttcc atattcgccg tgagtaacat
     2881 caacggtaac gataataaaa ataccaccca cgcataaacc gtcatcataa tgcctgccga
     2941 tgaaggctcc atttggaaac tgcttgcaat gtctgaaagc aatgcaatag gaataaattc
     3001 ggtggtgttt actataaaag ccgcaaaggc aagactaaat actctaagga acgataatct
     3061 ccgttgagct tttcg
//
LOCUS       DANLUA010000226         3039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLUA010000226 DANLUA010000000
VERSION     DANLUA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1965
                     /locus_tag="PXB44_002419"
     CDS             <1..1965
                     /locus_tag="PXB44_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL5282848.1"
                     /translation="LPELAFNDFSEDLFYSQPKNEGNYWYFDDQPHEVVVVNNLRKQP
                     EVGLMTGEITRGRNINALFDLLPESTIMAITIVIHPQDKLEAHLEKISSRAVGENFES
                     KYLKEDCQTVQSYLKEGHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVKE
                     NAEIAPLNSYLRWLPMNFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGITY
                     FNRGGAPFNFDPFNRKDRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVEA
                     GNSFGLLADYAERFGLTVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDND
                     DTDDEGDMQRDLLAEMELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSEG
                     RQTLTEDIKNALEALSHDEHTREERRTRLAEMAESLEMFCTGVNGQFFNREGAIWPEA
                     DLTLVDLAMFAREGYEAELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITANP
                     LLAKFLVKGSKMWRKLGIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEIN
                     RFRKLSEAQKTMLLSAKKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKAE
                     RKQLMMQHGYTELDAALEVAARIDKARGIH"
     gene            1965..2393
                     /locus_tag="PXB44_002420"
     CDS             1965..2393
                     /locus_tag="PXB44_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282849.1"
                     /translation="MNKQHYRLFNAGPQKSSLGVVSPAVETQLLGFCEQIRQLIGVGK
                     QITLFPHRVVQNHWAIELSGEQGSVHLLLNVTGRSLLPTSNNSEWSARLIIEIADHVD
                     AYWFSAFLTQYLGLKSNLLPLSTSIADNSVYNEVIDNNEV"
     gene            2383..2700
                     /locus_tag="PXB44_002421"
     CDS             2383..2700
                     /locus_tag="PXB44_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5282850.1"
                     /translation="MKSDTTKPKMTRVITVLKITLMIIGIILLLLVVGWLAMTYFPST
                     NFQAWFYETRYAWLGWRFVLYVAIWLLMVQLNRKGQMPLKARLMILSVIPIIEGLNLL
                     YLL"
     gene            2711..>3039
                     /locus_tag="PXB44_002422"
     CDS             2711..>3039
                     /locus_tag="PXB44_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5282851.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFVLPLIFVLIS
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRVPTA"
BASE COUNT          922 a          568 c          661 g          888 t
ORIGIN      
        1 cttccagaat tagcgtttaa cgatttcagt gaagatttat tttattctca accgaaaaat
       61 gaaggcaact attggtattt tgatgaccaa ccgcacgaag ttgttgtagt caataattta
      121 agaaaacaac cagaagtggg cttaatgacc ggcgagatta ctcgtgggcg taatattaat
      181 gcgttatttg acctattgcc agaatcaacc attatggcaa ttaccatcgt catccatcca
      241 caggataagt tggaggcaca tttagaaaaa attagtagtc gagcggtagg tgaaaatttt
      301 gagtcgaaat acctaaaaga agactgtcaa actgtacagt cttatcttaa agaggggcat
      361 aaactctatc aagcagctat tgcattttac gttcgaggta aggatgaaaa ggagctacgt
      421 acccgttctc gccagttgag aacgattctg ctcagtaaca acctagaacc cgtgaaggaa
      481 aatgcagaaa ttgccccact taacagctat ttgcgttggc taccaatgaa ttttaatccg
      541 cagatggatg ccaaatcaca ttactacacg aagtactatt ttgtacaaca tttggcaaac
      601 ttattaccta tctttggccg agataccggg actgggaatc cggggatcac ttactttaat
      661 cgaggaggag caccatttaa tttcgaccca tttaaccgta aagacagaac caaaaatgct
      721 cacaagctat tattaggtcc aaccggttcg ggaaaatcag ctacgttatg tgggcaatta
      781 tgtcaggcta tggccgttta tcgcccacgg acttttgttg ttgaggcagg aaattcattt
      841 ggtttattag cggactatgc agaacgtttc ggactgactg taaatcgcat tacgttagct
      901 ccaaacagcg gcattgtctt accactcttt tcagaagcac atcgtctatt ggatatggaa
      961 ttaaacccgg aagatgtgcc ggaagacatt gaggataacg atgacactga tgatgaaggt
     1021 gatatgcaac gagatttatt ggccgaaatg gagctgacgg cacgtttgat gatcaccgga
     1081 ggtgaaaaga aagaagatga aaaaatgagc cgtgctgatc gtgctgctat tcgtcgtgcc
     1141 attttaaatg cagctcgcca aaccaaatct gaaggcagac aaacgctgac tgaagatatt
     1201 aaaaatgctt tggaagcatt aagtcatgat gagcatactc gtgaagaacg gcgtacccgt
     1261 ttagcagaaa tggcagaatc attagaaatg ttttgtaccg gtgtaaatgg gcaatttttt
     1321 aatcgagagg gggcaatttg gccggaggct gatctaacct tagttgattt agctatgttt
     1381 gcccgggaag gctatgaagc agagttggcc atttcctata tttcactcat taaccatatc
     1441 aacagtcttg gtgaaaaata ccaacacagt gatcggatga ttctgaatgt aacagatgaa
     1501 tcacacatta tcacggccaa cccattatta gctaaattcc tagttaaagg ctcaaaaatg
     1561 tggcgtaaac tagggatttg gctatggctt gctactcaaa atatggatga cttcccgaat
     1621 gaagcggcga aattactctc aatggtcgaa tggtgggaat tgttgaatgt gtctgccagt
     1681 gaaattgatg aaatcaatcg tttccggaaa ctgtcagagg cccagaaaac gatgttgtta
     1741 tctgccaaaa aatcagatcg taaatatacc gagggcgtgg tattagcaac gaatatggaa
     1801 gctctattcc gagttgtacc gcctagctta tttttagctt taggaatgac tgaaaaacat
     1861 gaaaaagcag aacgtaagca actgatgatg cagcacggct ataccgaact cgatgcagct
     1921 ttagaggttg cagctcggat tgataaagca agaggaatac actaatgaat aaacaacatt
     1981 accgtttatt taatgcaggc ccacaaaaat cctcattagg agttgtatct ccagcggtag
     2041 aaactcaatt gcttggattt tgtgaacaaa ttcgccaact catcggcgtt gggaaacaga
     2101 ttaccttatt ccctcatcgt gtcgtgcaaa atcactgggc tatcgaactt tctggtgagc
     2161 agggttctgt tcacttatta cttaatgtga cggggcgttc tttgctacca acttccaata
     2221 acagtgaatg gtcagctcgt ttaattattg aaatagctga tcatgtggat gcttattggt
     2281 tttcggcatt ccttactcag tatctcggtt tgaaaagtaa tcttttaccc ttatccacat
     2341 caattgcgga taactctgtg tataacgagg taatagataa taatgaagtc tgatacaaca
     2401 aagcctaaaa tgacaagggt tattactgta ttaaaaataa ccttgatgat catcggtatt
     2461 attttattac tactcgttgt tggctggttg gcaatgacct attttccttc cacaaatttt
     2521 caagcgtggt tctatgaaac ccgctatgcc tggttgggat ggcgatttgt tttatatgta
     2581 gctatttggc tattgatggt acaacttaat cgaaaagggc aaatgccatt aaaagccagg
     2641 ttaatgattt tatctgtcat cccaattatt gagggactta atttactgta tctactttaa
     2701 ggaggatgag atgaccttta ccgtagatag ttacttagaa tactttctta ccttactggc
     2761 ttggatcatc aacaacaata ttttcgctgt attggttcaa acagggttgt ttgttctgcc
     2821 tctcattttt gtcttaatca gtacgtggat ggaagtcaaa aaacaaggtg aggacgaagg
     2881 taataaaggg gatttactta ttacttggct cagcctcaaa ttttacccgg cgatgtttgt
     2941 cgttgtattg gtcttagctc caatgatacc gatcaacctg aataatattg agttgaatgt
     3001 agaacgttct aaagcctgtg gttatcgtgt tccaactgc
//
LOCUS       DANLUA010000227         3011 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLUA010000227 DANLUA010000000
VERSION     DANLUA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..705)
                     /locus_tag="PXB44_002423"
     CDS             complement(<1..705)
                     /locus_tag="PXB44_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5282852.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVRVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYE"
     gene            complement(705..1808)
                     /locus_tag="PXB44_002424"
     CDS             complement(705..1808)
                     /locus_tag="PXB44_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5282853.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLASIREHKN"
     gene            complement(1880..>3011)
                     /locus_tag="PXB44_002425"
     CDS             complement(1880..>3011)
                     /locus_tag="PXB44_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL5282854.1"
                     /translation="SACSSLPTSGPSYSEILASNENTAETQLPEVNLIKLDNITVQNL
                     YQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPPAVLFGGTFSAEGQGNGHL
                     TQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGALSRKANQPQALVRIANNLS
                     SDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVRLTRGNKVRSMAFETL
                     IADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFSSKGITLAEAIGKMGGLID
                     ERSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPTVYQVNLLKPQSMFLLQRF
                     PIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
BASE COUNT          945 a          583 c          526 g          957 t
ORIGIN      
        1 ctcataggta attaccgttt caccaaaata accatgtcta aacatttctg taccatgaat
       61 cattggaaac cataaggcaa tattttgagc agttagcggt aaattatgga caaagaagaa
      121 tgcaccagaa agaggaagta gaaagaagct gaatgttccc caaatcttac caaaaggttc
      181 aatataatgt gctaaagtac agataattaa acctaaacct aaggcaaaaa atgccattaa
      241 tatccaagca cagagcatat agaaaatatc gttaggcatt tctatccaac caatagcgat
      301 aaatatagtc attaagagaa tttgggcgat agatgcgcca gtaacttcta ataaaactcg
      361 agtaaatatc gtatctagca ctctcacatt tctgtggtaa agcaaactta agtttgctga
      421 tattgctcct tgagctctat tagaggcatt tctccacatc attgcgatag ggtagcctgt
      481 aatcataaaa gcgataatat ttaaagttga aaacttatct gcttttatat atttccaagc
      541 aacagtaatc actaacgtta acaataatgg ttcgataaaa agccataaga agccaatatt
      601 tttacgccca taacgagtaa ttaattccct cattaaaagg gcgtgaatca cccgtttttg
      661 aatcacacag gaacgtttaa aggttgtatt atcaccatat cgcattagtt tttatgctct
      721 ctaatacttg ctagtaacaa acttaatact ccaaatagaa ttaagccgat aaaaaaagtt
      781 gatataatat tataaattct gtagggttct aatgcccaat ctggtttgct tggcctacca
      841 attacctcta aataaagttg ttggcgatca gcttcacctc gtgtattttg taatgaagtc
      901 attgctgctg caagttgttg ctgagctaat tcgttagcaa gaaccaaacg ttgatattca
      961 gcagtttgcg ttgctacaga agtattacct cctgcaagtt gtttggactg atcatcaatt
     1021 tcttttttca aacttttttg acgcatttgt aatgcggcta cctgaggatt atccggcgta
     1081 attgacttca attgtgctaa ttgtgtttct acccgaatta attcactttt taaggtagaa
     1141 attaatgaga gctgaacgct ggattgtgcc ggtaaatcat aaattttatt tttagttcga
     1201 tattggctta atgctaacgc agtattattg acgtcttttt cggcctcttt tacacgctct
     1261 tctgcaaact caaccgtatc ttgtcttgct ctttcattaa ggcggttaat taatgtttcc
     1321 ccttgctgta ataaattctc gttaattttt tgtccttctt ctgcattaaa agcacgaacg
     1381 cgtagcgttg caatacctga aatagaatcg acatcaatat tcagacgttc tttaaaataa
     1441 cgataaaatg cttcttgggt atcgttaaat cctaaaccgt taaatttgct tagtaaatca
     1501 ccttgctcac tataaaaacg ctttaccgga atttctgctt caagttgttc taatgcggta
     1561 cgggaacgca tatattcctg aactgaatag gtatcatctt gagaacgggc gaaaccggag
     1621 ttttgcaata aagcaccaac gcctgttaag ctcgattgat tattgggcga acgaaccaca
     1681 aagctagatt ctgaaacata aatatcagag gcaaataagg aataataaat tccagaacaa
     1741 gcagttggaa taattacagt tagccacaac aaagggttgg ctaaaaaatg ttttttcttc
     1801 tttgacataa aatcctcaac gaaaagtgtt acaagcggtc attttttaat gattttttac
     1861 cattttatct atgagctaat tagtacgctc taattgtatt agctgtacct gtaattggtg
     1921 tagtaatgga gaaaatcatt ctcaagaatt tttgaaattc agacagtgga gcattcgaca
     1981 catatacaat atctttatct tgaataggga aacgctgcaa taaaaacatt gattgcggtt
     2041 ttaataaatt cacctgataa acagttggaa cgtccattcc ctctgcataa cctttttcac
     2101 gccattgagc ttggttatcg taatctaatt gtgtaaaagg aatatggcgg aatacgaaca
     2161 caccacgagg gtctgaacgc tcatcaatta atcctcccat tttaccgata gcttcagcaa
     2221 gggtaatgcc tttacttgaa aaacgcattt gttgattatt acctactgct cctaatccgg
     2281 taaagctata aggagtattc attaaagcaa taacatcacc tgaacgtaat gtaatatttt
     2341 gtgaggggtc agcaattaat gtttcaaacg ccattgaacg cactttattt ccacgtgtta
     2401 aacgaactgt tacatcttca atattttcgc ttgtaccacc tactgccgca actgcatcta
     2461 aaatacgctc attattggca cttaatggca tacgaatgct atttccttgg cggataaccg
     2521 taacatcaga cgacaagtta ttcgcaatac gaactaatgc ttgaggttga ttagctttac
     2581 ggctcaatgc tccgacaatt tgattctgaa ttacttcagg tgtttttcca gcaactttaa
     2641 tattaccgat aaaaggcaca gttaccgttc catttttgtt caccatttgc tgaggtaatt
     2701 gggttaaatg cccattacct tgcccttcag cactaaatgt tccaccaaat agcacggcag
     2761 gtggcgcttc ccaaatggat atttctaata tatcgcctac attaactgtt ccagcataac
     2821 cacccacacc cattgagcca tcaaatcctg aaaaacgttg agattgttgc tcttggtata
     2881 agttttgtac ggtgatgtta tctaacttga ttaaattgac ttcaggcaac tgcgtttcag
     2941 ctgtattttc attacttgct aagatttcgc tatagcttgg gccggacgtt ggtaggctag
     3001 agcaggctga a
//
LOCUS       DANLUA010000228         3011 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLUA010000228 DANLUA010000000
VERSION     DANLUA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(76..1671)
                     /locus_tag="PXB44_002426"
     CDS             complement(76..1671)
                     /locus_tag="PXB44_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5282855.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            1820..2260
                     /gene="mioC"
                     /locus_tag="PXB44_002427"
     CDS             1820..2260
                     /gene="mioC"
                     /locus_tag="PXB44_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL5282856.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            2272..2439
                     /locus_tag="PXB44_002428"
     CDS             2272..2439
                     /locus_tag="PXB44_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL5282857.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(2528..3001)
                     /gene="asnC"
                     /locus_tag="PXB44_002429"
     CDS             complement(2528..3001)
                     /gene="asnC"
                     /locus_tag="PXB44_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL5282858.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVE
                     AYYTTGNYSIFIKVMTHTIEELHSVLAGKIQSIDEIQSTETLISLQNPISRDILP"
BASE COUNT          887 a          630 c          593 g          901 t
ORIGIN      
        1 ttctaaaaga aaagcagtct attctctacc acattttgca aaaaagtggc aaaaatagac
       61 cgcttgtatg gcatatcact ccgataaact caccccataa aatggcgtgt agccaaacgg
      121 attttgcaca aaaccttgca cccgtttgct gagtggtaca taattgatag cgtgagccag
      181 cggaataaat ggcgtttgtt cgttggcaat ttccaccgct tgttgataga gcttcgcccg
      241 ctcgtcacgg ttttgggttt gtacagcttg agctaataac tgctcaaatt tctcatccgt
      301 ccaacgccca taatttgttc ccggaatatt agatttgctt aataaaggga agaggaaatt
      361 atcaggatca ccattacggc ttgtccaacc ataagtgccg gtagagaact cgccttcacg
      421 ggtgcgtttg ttaaaatccg cccattcgtg ggtcactaat ttcgctttca ccccgatttt
      481 cgcccaatcc gcctgaataa tttcagccgt tcgacgtggg tttgggttag aagggcgcac
      541 taccggttgt acccaaattt ccgtttcaaa tccatcggca aagccggctt ctgttaatag
      601 ggctttggct ttttcaaggt taaattcata ttgcggtaaa tttggattat agcccaacac
      661 cgaatcaggg aacggattac ttgccaccgt gccaccgcct tgataaacag cctcgacaat
      721 cgctttctta tcggttgcat aatgcaaggc ttggcgaact ttggcgttat ttaatgccgg
      781 tttggtgctg tttaagccga tataagctaa attcagcccg tctctctgta acaaattcac
      841 cttcggatct tgtttcattt gggcaatatc ggaaatattc gggaaatcaa tcacatcgca
      901 ttcacccgct ttcagtttgg cataacgagt gcccgcatca ggggtaatac taaaaatcag
      961 tttctcaaat tttgctttac cttggaaata atccgggttc gcaacataac gcaccgcttg
     1021 atcggtttga taggcttgga acacaaaagg ccctgtgccg atcggggctt gatccaataa
     1081 ctccggcttg ccttccgcca gcaatttatc cgcatattct tttgaataaa tcgacaagaa
     1141 atccatcgct aaggttgtca gaaaaggagc attaggttgc tgtaaggtga ttttaacagt
     1201 gtaatcgtcc actttttcca ctgtttttaa aatcttcggc aaattcatcc aatggtaata
     1261 gaaataagta ccgtcagaga ccttatggta agggtggttt aaatccatct ggcgttggaa
     1321 cgagaacact acatcatccg cattcagcgt tcgagttggc gtaaattgtt tattggaatg
     1381 aaatttcacg tctttacgta aatggaatgt gtaacttaag ccatcgtcac tgatttccca
     1441 acgttccgcc aaactcggct caaccgttag ttcaccggct ttaaacgcta ataagcgatt
     1501 ataaatttgt tgtgaactgg cgttgaaatc attggcatca ttagtaattg caggactaag
     1561 tttcatcggt ggagttgcca cgcaattaat caacgtattt accggagctg ctgccaccaa
     1621 actgctggca cttaagagta aaacagaaag agataattga gaaagacgca taaagactcc
     1681 ttaacatttt ctactaactt actcggcatt aacgtatttt caaaataact aaaggttata
     1741 atttataatc agaataatca gttgcaaacc aatgattctt ggctagaata ttggcagttt
     1801 tttaaacgag agatttacta tgacatcaat ttgtatcatc accggcagca ccttaggcgg
     1861 tgcggaatat gttgccgatc acattaatga ggtattaagc gaacaaggtt ttgaagtgga
     1921 actgtttaac caagccaccc ttgccgatat tgagggcaaa accaatctga ttatcgttac
     1981 ctctacccac ggagccggcg agttacccga aaatatccaa cagctatttg acgatttagc
     2041 aaatagctcg gctgatttca gcagtatgaa atttggtgta atcggtttag gtagctccga
     2101 ctatgacacc ttctgcaatg cagtaaatat tgttgaagaa gctctcaaag cgaaaggggc
     2161 aagccaagtt tgcgaatcac tgagaattga tgtaatgaat aatttcgacc acgatggcag
     2221 tgctgaagaa tggttaccat catttgttga acaattataa cttaagtatc aatgaacaaa
     2281 atcagtgcaa ataaggcaaa gtggattaaa atcgctatta tcttaatcta tatgttcagc
     2341 cctgtagata ttctacccga agcgatttta ggcccattcg gtttggttga tgatgccgcc
     2401 gcattaatgc tattgattaa gactattttg gaaaaatagg catagtagac aaaatagttg
     2461 caaaaaacca aaacaaaacg accgcttgta acccaataca agtggtctta tcttttactt
     2521 aattctttta agggagaata tctcttgaaa taggattttg aagtgaaata agcgtttctg
     2581 ttgactgaat ttcatcaata gactgaattt ttcctgctaa taccgaatgc aactcttcga
     2641 tggtatgggt cattacttta ataaaaatag aataattacc agtggtatag tacgcctcaa
     2701 caacttcatt aaattcacta agtttagcaa ttactttatc ataatctttg gctgatttta
     2761 aaataatgcc aataaagcaa cacacgtcat acccaacctt acgctcgtcc actacgattt
     2821 tggtagattc aataatgccg gcttggcgca ttttttcaac acggacatgg atagttgcgg
     2881 cactcacacc aaaaactttt gccatttccg cataaggcgt gcgagcatct ttaattagcc
     2941 aacgtaaaat tttttgatcc agcttatcaa tttccgattt agtcgtgttt ttactgtaca
     3001 ttttttctcc t
//
LOCUS       DANLUA010000229         2959 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLUA010000229 DANLUA010000000
VERSION     DANLUA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..560)
                     /gene="pdxT"
                     /locus_tag="PXB44_002430"
     CDS             complement(<1..560)
                     /gene="pdxT"
                     /locus_tag="PXB44_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL5282859.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCR"
     gene            complement(560..1432)
                     /gene="pdxS"
                     /locus_tag="PXB44_002431"
     CDS             complement(560..1432)
                     /gene="pdxS"
                     /locus_tag="PXB44_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL5282860.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1643..>2959
                     /locus_tag="PXB44_002432"
     CDS             1643..>2959
                     /locus_tag="PXB44_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL5282861.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQN"
BASE COUNT          911 a          605 c          550 g          893 t
ORIGIN      
        1 cggcacattt ccacaaaata gcggtgaaaa cttaaatcat tattcacttc gggatggaag
       61 gatataccta tctgattttg gtattttact ccaacaatct taccgcttat cttggctaaa
      121 atttctacat cttttccagt gctttctata taaggcgcac gaatgaatgt gagaggaatt
      181 ttaccgagat atttaacttc ttcttctact aaaaacgagc ctaattgcct tccgtaagca
      241 ttacgtttca ctgttacagg caaagtagcc aaatgttttt tatcgtcgtt taccaaattt
      301 tccgcaagca aaattaaacc cgcacaagtt ccaagcgttg gcaagccatt taaaattttt
      361 tgacgaagcg gctcaagcaa gcctaattca cgtaataatt taccttgaac agtcgattcg
      421 ccaccaggta aaatcagacc atcaaaatct tggtttaaat cagcaagttg acgaatttcc
      481 atactttcaa cccctagttg ctggagttta tcagtatgct cgctaaatgc accttgcaga
      541 gcaagaatag caattttcat tagacacctc ttgctgccat tagcaattca atctcttgct
      601 cattaattcc taccattgct tcacctaaat cttcagaaag ctccgcaagt aatttaggat
      661 cgttgtagtt ggtaacagct tgtacaatgg cccgcgctcg tttagcagga tcgcctgact
      721 tgaaaatacc tgagccaacg aagactcctt ctgcacctaa ctgcatcatc aatgcagcat
      781 cggcaggggt tgcaacacct ccagcagcaa aggtgacaac aggcaatttt tggtactgat
      841 gtacatattg tactaaatca aatggtactt ggagctcttt agctacgtga taaagttcat
      901 ctgtggtatg tgaaaccaag cgggcaattt cttgtttcat tttgcgaaga tgatgtactg
      961 cttgcaccac attgcctgtt cctggctcgc ccttactacg gatcatagca gcgccttcat
     1021 taatacgacg tagggcttcg cctaaatttt ttgctccaca tacaaatggc acttcaaatt
     1081 tacgcttgtt aatgtggaag gtatcatctg caggagaaag cacctcactt tcgtcaatat
     1141 agtcaatttc gatcgcttgc aggatttgtg cttctgtaac gtgtccaata cgtactttcg
     1201 ccattacagg aatggatacc gctgcttgga tactctttat catcttggga tcactcatac
     1261 gagaaacgcc accagctgca cgaatatctg caggaattct ctctagtgcc attactgcac
     1321 aagctccagc ttcttccgca atgcaggctt gctcaggggt agttacatcc ataattacac
     1381 cgccttttaa catttgagca agttgtctgt ttaaatcata gcgattagtc attgtacttt
     1441 ttccttctaa ttgttgaata attgaacttt tcgtagtttc gattagctcg gcattaagtt
     1501 tatttttata ttcgctggat gcgagtgttc atctaccaat ttaaatacac tcagccaatt
     1561 tgagtgagga aacagcatgt attgtaattt aatctgaact gtttaaaatg accattttat
     1621 gtttttctta tcatgtcaga ttatgcttac ctacactttt aacacacaag aaggcaccgc
     1681 tctctatgag caactttacc gttttctaaa aaacgacatt gaaagcaaca tacttatagc
     1741 aggggataaa ctgccgtcta aacgggcttt tgcaaagcat ttaggcattt cagtaatgac
     1801 agtggaaaca gcttaccaac agttagtagc agaaggttat gtgaatactc agcctaaaaa
     1861 aggctttttt gtgaatgctt taaatttacc agaaaaaaag gaagccatat cagttcagct
     1921 acaaaaaatc agttcagaac agtatgagaa aaataaaaaa tggcaggcag atttaactaa
     1981 tcgacagact ttagcagaaa attttccgtt ttcggtttgg acaaaattag tacgcgaagt
     2041 gctgaaaaca caccaaagtg agctaatgga gcgtgctgag agtgggggcg tatggacatt
     2101 aaaatttgcg atagccaatc atcttcgagc ctttagagga atgatcgtta atccaacaca
     2161 gattattatt ggagcgggta cggagtatct ttacggtttg ctcattcagc ttctaggctt
     2221 agataaacat tatgcactaa ctgagcccag ttacgataaa ttgcgcagaa tttatcacag
     2281 ttatcgctta tcaacagcca cagtatcgat gccattttca atcgatgagt taaattcaaa
     2341 gaatgtggac gtgatacata cctcgccatc acaccatttt ccgacaggac acgtaatgcc
     2401 gattgccaaa cgttatgagt tgttagcttg ggttgcacaa aaagctgaaa gatatattgt
     2461 agaagacgac tacgacagcg aatttcgttt tgtaggacaa ccaattcctg cattgaaatc
     2521 tattgatatg ctcgggcgag ttatctatat gaatactttt tctaaaacgc tctcttcaac
     2581 tgtacgaatt gcctatatgg ttttgccacc agaattactg aatcgttttc agcaggaatt
     2641 agggttttat gcttctaccg tttccaattt tgagcaatat attttggcag aatttattca
     2701 acaaggctat tttgaaaagc atatcaatcg aatgcgagcg tattatcaaa aaaagaggga
     2761 taaattacta aaagctttca agcaaggcaa tttagcagaa aagattgcaa taaaagaaga
     2821 acattcaggc ttgcatttca ttgtacagct aaacacaaca ttaacagaaa ggcaaatttt
     2881 gcaggcagca gaagaacaag gtataaaatt tgtcgcattg tctgcttatt atcaagataa
     2941 atctcaaatt cagcaaaat
//
LOCUS       DANLUA010000230         2949 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLUA010000230 DANLUA010000000
VERSION     DANLUA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(82..1056)
                     /gene="ssb"
                     /locus_tag="PXB44_002433"
     CDS             complement(82..1056)
                     /gene="ssb"
                     /locus_tag="PXB44_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5282862.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(1059..1256)
                     /locus_tag="PXB44_002434"
     CDS             complement(1059..1256)
                     /locus_tag="PXB44_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282863.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            complement(1240..1425)
                     /locus_tag="PXB44_002435"
     CDS             complement(1240..1425)
                     /locus_tag="PXB44_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282864.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            2071..2667
                     /locus_tag="PXB44_002436"
     CDS             2071..2667
                     /locus_tag="PXB44_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282865.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            complement(2703..>2949)
                     /locus_tag="PXB44_002437"
     CDS             complement(2703..>2949)
                     /locus_tag="PXB44_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282866.1"
                     /translation="MRFRNRLYHQEVVWNKRIAKKPTHALENLEKTYKQFELALQKIA
                     PERFAFRQLSQALIWQRQIFFDQQIFEAEIVILPQHI"
BASE COUNT          825 a          538 c          590 g          996 t
ORIGIN      
        1 tacccttata tttcaaatag ttacatttaa atatcagtta ttaatttcct atgtcaaaaa
       61 tactttgtga ctaatttgtg actagaattt aactgagtta gcgtggttga gtaaatggtc
      121 tgcattaaga tgggcatatt tctttaccat ctctaatgtt tcccagccac ccaattcttt
      181 taaggtaaat aatggtgtgc cggcctgaac gtgccaactg gcccaagtat gccttaaatc
      241 atggaaatgg aaatcaacca ataaacattt ttcagtcgct ttgttaaata ttttgcggtt
      301 aatatcttgt aatgcctgcc ctttgcttcc tacaaaaaca tatttaggat ttttccctct
      361 ctgcttttgt aataattcaa ttgcatcatc attaagtaaa agcgatctcg ctttttcaga
      421 tttggcaata tcattcgaaa caatcgctac tttacggata aaatctattt tatcccaagt
      481 catagaaagt atttctgttc gccttgctcc tgttaataaa gcaaaagagc agatagtttt
      541 catccaatca gtactaatat tattaataaa ctgcttggct tgttctttgg taatccaacg
      601 aacccggata tcatcatcaa aatctcgtga ttctgaatcc gtcattacgt ggttatatgg
      661 attggttggt ggtgggtttc ctgccggttc ggttgcccaa ttattattgc tgtttccgct
      721 gtttgagcta ccgagcattt gtagctgatc ggcgatgatt tcggtcgtcc agcggtctgt
      781 gccgtcttgg gcttgccatt tgcgagtgcg tagcttgcct tccacataca cttgcgagcc
      841 ttttttcagg tatttgccga taatgtcagc taaattccga aaggcgataa tgctatgcca
      901 ctctgtggct tgttttttct cgcctgaggt tttatccgtc cattcggttg aagttgctac
      961 actgattttg gcgactgaat cgccatttgg cattgttcgt agttccgggt cgttacctag
     1021 gcgaccgaca ataatcactt tgttaattcc tgccattgtt attccttata aagtttgatt
     1081 ggtttgtctc tgccaatgtt gaaccgctca attttgcggt taatcaacat aattgcgttt
     1141 tggcggttgt attgtggata ggttgtgtgc tgtaatttgc cgtcataaaa aaacttggcg
     1201 ataaatcgtt tatctacctc aacaatctca acaccggcat caatttttcg tgccatattt
     1261 ccctccgcag tattggttgc cgttgctttc catttgccaa aagccttgat ttcgttcgca
     1321 tttgtaacgt tgcatttgta tgtggtcgtc ataatccatt cgcccgacaa tgcctaatag
     1381 caaaattagc aaggcaaatg gggcaagttg tttcattttg ttcataggat tgtcctttag
     1441 ggttaaaaaa agcccacgtt ttacggtggg caaatggagc gacttgcgtc tagttgtggg
     1501 tattccgaaa cacacttggt gaaatgagct ttggaataaa tgcgtgtctg cccacgccgt
     1561 cgcccgcccg gctttcgcca ttccgtcggg tgctgcttgt atccgtactt actcaaactt
     1621 gagataagcg tgattgctta ggactgatac cagtgttgcc ttttcagctc tccacacctt
     1681 gcgagtgtgt tgcctaaacc ttgccacctc tttttggcaa gtcgttcaaa tttgtatgtc
     1741 agggtctaac caaccttact gagttataaa gcattacttt acaactgccc gaggttgtta
     1801 atatcgtccg cacaagcacc gaattgttaa agagcattga gccgtagctc gttttgatga
     1861 attaagttta agaaaactta aattacaagt caatcaaaat ttaagcttat ttaataagta
     1921 tttttaagat ttcttaaatt tcaattagtt atgatttctt aattgattgt tttttaaacc
     1981 attaaaatat aattattgag attgcgagct tgatcacaga ccgaaaacca cgcttttgag
     2041 aaaataagaa gggatttcta tggaggttat atgaaaaaac tactgatgac tttatgttgt
     2101 ttgcccgctt ttgctttagc taacgaaact ggagaaagtt gctctaagat tgaagatagt
     2161 gcaaaacgct tagaatgtta tgatagtgtc tttgtgaaaa aagatactgg taaagatgaa
     2221 gctaccgatg aaaagtctaa ttgggaatat gaacaaaaga aagatgaatt gcgtaatgca
     2281 acgacttact ttgctaaaat tagatcaact aacacaatag attttggttt cccgtataat
     2341 tcatcatcaa tgaatttaat gttacgcaaa gaccctaaat atggcaatga tgtaattttt
     2401 agcgtacacg gtcaatttaa tggctgtatg attgagagct gtaaaattac tgtgaagttt
     2461 gatgatggta aattggaaag ttatcgaatg attggggcgg atggtggcag caatgatacg
     2521 cttttcattg aaaatgcaaa agcaatgaaa acctttgtag ataagttgaa aaagtcgaaa
     2581 aaactaattg tagaggcgag tttttacaat tatggcaaag ggcaatttac ctttgataca
     2641 caagggttag agtggaaaca tttttaatta agaaaaagct acatagatgc gtagcttttt
     2701 gcttagatat gttggggcaa aataacgatt tcagcttcaa aaatttgttg gtcgaaaaag
     2761 atttggcgtt gccaaataag tgcttgtgaa agctgacgaa acgcaaatcg ttcaggtgct
     2821 atcttttgta gtgctaactc aaattgcttg taagtttttt ctaaattctc tagtgcgtgc
     2881 gttggtttct tggcaattcg cttattccaa accacttctt gatgatacag ccgatttcta
     2941 aaacgcata
//
LOCUS       DANLUA010000231         2926 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLUA010000231 DANLUA010000000
VERSION     DANLUA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(31..821)
                     /locus_tag="PXB44_002438"
                     /pseudo
     CDS             complement(31..821)
                     /locus_tag="PXB44_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(818..1519)
                     /gene="lpxH"
                     /locus_tag="PXB44_002439"
     CDS             complement(818..1519)
                     /gene="lpxH"
                     /locus_tag="PXB44_002439"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL5282867.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            complement(1594..2115)
                     /locus_tag="PXB44_002440"
     CDS             complement(1594..2115)
                     /locus_tag="PXB44_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL5282868.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            complement(2125..2913)
                     /locus_tag="PXB44_002441"
     CDS             complement(2125..2913)
                     /locus_tag="PXB44_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL5282869.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
BASE COUNT          858 a          585 c          558 g          925 t
ORIGIN      
        1 tttttttagg gaaaatctgc aaatgctttt ttagctccat tcaagcgtat aaggcttaat
       61 accaatccct ttactattaa gacagtcttg tagatttcta atattattaa caatattttc
      121 ggtaattagg ttggcttgtt taactttttc ttcagtaaca ttgggatctt tttcgttgaa
      181 taaaatcgca tttaataatc tttctgcgtg cataccctct cggcagtaat gtaaaacacc
      241 gacatcatta aatgcagtag ctacagagct aatttgtgta gtggctatgc ctaaagcacc
      301 atcttctcca cctaattctt ggaagagctg atgttgtgga aagccgccac aagcgaggaa
      361 gaaactacgc cgaaatacag tgttgcctgc accagtcatt tgcatatatt gccaagctaa
      421 ctcaaaatta gggtgagagc tgtaacgttg atctaagtta ccggttttaa tgctaaccga
      481 accaagccgg tttccggttt aaactcaaaa atcttttcag caacttgcaa tgccccgttt
      541 tcataagcat catcagcatc taaaaaggca attaaatctg tttcactttg taatgctccc
      601 cagttgcgtg ctttggcaac accgccattt tgaggcattt gttccactct aattttatca
      661 ggcacttggg cttgtaaatg tttagctaag gcaagcgtat tatccgttga agcatcatca
      721 ataagccaca atagatttaa tccagactga tttaatacac tttgcactgc acgaacaagg
      781 gtgttttcgg cgttatagca aggaataatc acattaatca tataaattca gctccttgct
      841 cggttacgct taaaatagag gcgtaatttt ctttccagtc gcctaataca atgcgggtaa
      901 atccattatg ctgatggatt gcttgtcgat gcgtatgccc gtgtataagt tgagttgtat
      961 tgtattttgc gacaatttga gcggtaactt cagcgtttac gtccataatt tcagcggatt
     1021 tatcacgctt ttcttgtttg cttttggatc gaattttctc cgcaatacga attcgccaag
     1081 aaagcggaaa catcaaaaag atagcttgtc gccatttttg gtggactttt ttacgaaatt
     1141 gttgatattt cacatcatca gtacaaagcg tatcgccgtg gcaaagcaag gtagatgtgc
     1201 cgaataaatc tataacgtga taatcaggca aaataaccat acccgtttgt tcgctgaatc
     1261 gtttgccaat taaaaaatca cgatttccgc aaataaaata gcattttgta ccgcttgtag
     1321 tgagggtttt gattgcctgt ttaacggttg aaatgagttc agactgctca tcatcgccaa
     1381 tccagaaatc aaacagatca cctaagatat aaaccgcttc tgcaagtagt gctttttctt
     1441 gcataaattg caaaaagagg tgggtaattt caggctgatt ttcatttaag tggagatcgg
     1501 caataaaata gtacgtcatt tgatattgtt gataaaaaag aaaacggttc aattttttac
     1561 aaaatcgaac cgtttgtcaa aggttaatcg gcattaatct ttaaaaatgc cacgaataaa
     1621 ttgtactgag aggaagaaca agaacgccaa tacggcataa agtacatagc tcataattgg
     1681 tttgctgact gtttctgctt cttctaatgg cttgattgaa acaatatttc taaattcatt
     1741 tagccaattg attcgccagc cgtaatattt aatttgcaca cgttgttttt cgttagcgta
     1801 ggcttgagct ttggcttgaa tgtcggcaga tccgaatttg aaataaggag gcaaccccca
     1861 gccggtatct tcattacgat aaaccattac ctttttggtt tcaggatctt ccgtgaataa
     1921 gaaataaaca tcgcgcacct cgccatcagc aggattcgct ttgctgataa taccatcttt
     1981 atccattcgg cgtacttcca tacctgttac tacggtatct tcataacttg gcattgcata
     2041 attaactgca ccgcctaata caataaaaaa tgatagtgag acaagaatca gaaaatactt
     2101 aagtattgct ctcatattag ttccttataa tttaatgttg aataagtgtt gcacattttc
     2161 ggttgtaatg cgagctaatt cttcagttga aacgcctttt aatgtcgcca catattcgca
     2221 agtttcccgc acatacgccg gctggttcgg cttaccacga taaggaatcg gggcaagata
     2281 aggggaatct gtttcaacca ataagcgatc taacggcact tttcgcacga cttctcggag
     2341 ttcttctgca ttgcggaaag taataatgcc cgaaattgag atataaaagc cgatgtctaa
     2401 agcccgtttt gccattgccc aatcttcggt aaagcaatgc aacaccccac cgcatttttc
     2461 ggcattattt tgttccaaca tatccatcgt atcttggcgt gccgaacgag aatggataat
     2521 gagtggctta ttgagtttgt ttgcaatttc aatttgttga acgaaaagcg attgttgcaa
     2581 cgctttggtt tccggtgtgt agtgataatc caaaccagtt tccccaatgg caaccacttt
     2641 cggatcttga gcgaactgaa ataatttatc gtaatcaaaa ggttcatctt ccacattcaa
     2701 cgggtgaacg ccacaagaaa gtgaaacgtc attacgatgg gcggttaagt ttttcattgc
     2761 ttcaaaacgc ccaactgtag tgcaaaccga gataaaatga tgcacccctc gggctttggc
     2821 attttcaatt acttcgtcca cgtttttgtg acgagtttcg tagtctagag aatctagatg
     2881 acaatgtgag tctatgataa ataagtcttt catttttcta aatata
//
LOCUS       DANLUA010000232         2917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLUA010000232 DANLUA010000000
VERSION     DANLUA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            59..1126
                     /locus_tag="PXB44_002442"
     CDS             59..1126
                     /locus_tag="PXB44_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282870.1"
                     /translation="MKFILFDSILERHLGESLKRALESLGHQAVFTDLILHGHSMIYK
                     QADIDFMWKKVKDICDEKADMFISFRPMNLTTEMLEYIGKRMKTAIWLSDDPVLYKTC
                     YSQVVNHYDVLLHCGYKEVMDFYEANNHPKGFNFPFWTDHVAFPSVYNPLHSDYEIAF
                     LGNMNGQVRRKRYMELASLPFTKKVFGLIDSDPLAMHGGFINEAYLHTKRVTEVLSKA
                     KVGVSIPQFFTEYNGLDYDYPELAGLGYFQFPSRVIQYAASGLPIAAVGDERMKEVYP
                     EIFVGNSITELEPYIKQICEDYDFALSESAKILTRFRKNYSALSRAMMLIDLVENLDK
                     LQSQTTQERAILFTKYKAQYN"
     gene            1138..1944
                     /locus_tag="PXB44_002443"
     CDS             1138..1944
                     /locus_tag="PXB44_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282871.1"
                     /translation="MNIKDLLKEISFKAVINDLNFSSCLYIKPNTSKLYVILNGALIK
                     EPRNPLIYHRHSWHLEFDGSVLYIADPTLFKYESINLAWYVGDKDTDLYPYLAEFVTS
                     VATHLNVETDHIVTYGSSGGGFAALQLGARIGNGVVTVCINPQTNIMKYVPSHRNAFL
                     NACFDFNAEEYDSLSNQEKFNALVLLKENTSKILYIQNTQDEFHHKWHFLPFLELKGI
                     DLSEIEKTHWREQQSKDVKVILYSHESGHAAEPKEMIPEILSAVDQLIAS"
     gene            1946..>2917
                     /locus_tag="PXB44_002444"
     CDS             1946..>2917
                     /locus_tag="PXB44_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5282872.1"
                     /translation="MLFYLVSDSLTRLSLSNESLVDLGRITSIGNYPFLHNAKNAKFL
                     MVESAWQGYKNRWKYKIASYPDNPERTNEKLVRLVEKAKDKGIPTVFWNKEDSVHFDR
                     FIDSAKHFDHIFTVDQNAIARYREVVPATTTVNVGMFPVQPKIHNFQGFNFKHLAANF
                     VGSYSHHIHNKRRERQDMLFGAALKAGLPVTIFDRNSDRKSENYRYPKNSFNLNVQPN
                     VSYTETANIYRDYLVSLNINTVEDSPTMFSRRVVEILACGGILVSTPSMAIDNIFKEY
                     CHIVQSEDEAIELFQRLKRGASRDDLNKAIAGSEFVLSNFTWEHFLSQ"
BASE COUNT          963 a          450 c          545 g          959 t
ORIGIN      
        1 ggtagagatt ccttacttaa attggtttat aagacttctc catatttagg ggtttaatat
       61 gaagtttatt ttatttgatt ctattttaga acgtcatttg ggcgaaagtt taaaaagagc
      121 tttggagtcg ttagggcatc aagcagtatt tacagattta attttgcacg gccattctat
      181 gatttataaa caagcggaca tagatttcat gtggaaaaaa gtaaaagata tttgtgatga
      241 aaaagcagat atgtttatct catttcggcc gatgaatcta acgacagaaa tgcttgagta
      301 tataggcaag cgaatgaaga ccgctatttg gctttcagat gaccctgttt tatataaaac
      361 ttgctattct caagtagtaa atcattatga tgtattatta cattgcggat ataaagaagt
      421 tatggatttt tatgaagcta ataaccatcc aaaaggcttt aattttccgt tttggacaga
      481 tcatgtagca tttccaagtg tatataaccc gcttcattct gattatgaga ttgcattttt
      541 aggtaatatg aatgggcaag ttagacgcaa aagatacatg gaattggctt cattaccatt
      601 taccaaaaaa gtattcggtt taatagattc agatccatta gcaatgcatg gtggatttat
      661 caatgaagca tatttacata caaaacgtgt aacagaggtt ttatctaagg caaaagttgg
      721 ggtgagtatc ccacaattct ttaccgaata taatggttta gattatgatt atcctgaatt
      781 ggcgggttta ggatatttcc aatttcctag tcgagttatc caatatgctg caagtgggtt
      841 acctattgca gcggttggtg atgagagaat gaaggaagtt tatcctgaaa tctttgttgg
      901 caatagtatt acagagctgg agccgtacat taaacaaatt tgtgaagatt atgattttgc
      961 actatcagaa tcggctaaaa ttctaactag atttagaaag aactattctg ccttatcccg
     1021 tgccatgatg ttgattgatt tggtagaaaa tttagacaaa ctacagagcc agacaacaca
     1081 agagcgcgca attttattta ctaaatataa agctcaatac aactaggagt aaactttatg
     1141 aatattaaag acttacttaa agagatcagc tttaaagctg taattaatga tcttaatttt
     1201 tcatcgtgtc tatatatcaa acctaataca tcaaaattat atgtgatatt aaatggtgca
     1261 ttaatcaaag aacccagaaa tcccttaatt tatcatagac atagttggca tctagaattt
     1321 gatggctcag ttctatatat cgcagatcca acattattta aatatgaatc aattaatttg
     1381 gcttggtatg ttggagataa agatactgat ttatatccat acttagctga atttgtaact
     1441 tctgttgcta cacatttgaa tgttgagaca gaccatattg ttacttatgg ttcaagtggt
     1501 ggtggatttg ctgcattaca gttgggagca agaattggaa acggtgtagt taccgtttgt
     1561 attaatccac aaaccaacat catgaaatat gtaccaagtc atcgtaatgc ctttttaaac
     1621 gcttgctttg actttaatgc ggaagagtac gatagtcttt ctaatcaaga gaaatttaat
     1681 gctttagtat tacttaagga aaatacatca aagattttgt atattcaaaa tactcaggat
     1741 gaatttcatc ataaatggca tttcctccca ttcctagagt taaaggggat agatctatcg
     1801 gaaatagaaa agacccattg gagagaacaa cagagcaaag atgtaaaagt tattttgtat
     1861 tctcatgaat caggtcatgc agctgaacct aaggaaatga ttcccgagat attatccgct
     1921 gttgatcaac tgatagctag ctaaaatgct gttttatttg gtatctgatt ctttaactag
     1981 gctttcatta tcaaatgaaa gcctagttga tcttggaaga ataacatcta taggaaatta
     2041 tcctttcttg cataatgcaa agaatgctaa atttcttatg gtcgaatccg cctggcaagg
     2101 ctacaaaaac cgttggaaat ataaaattgc ctcttatcct gataatcctg aacgaaccaa
     2161 tgaaaagttg gttcgtttag tagaaaaagc gaaggataaa ggcattccaa cggtcttttg
     2221 gaacaaagaa gattctgtgc attttgatcg atttattgat tcggcgaagc attttgatca
     2281 tatttttacc gtagatcaaa atgccattgc tcgttatagg gaagtagtgc ctgcgacaac
     2341 aaccgtcaat gtcggaatgt tccctgttca gcccaaaatc cataactttc aaggatttaa
     2401 ttttaaacac cttgccgcaa attttgtagg gagttacagt catcatatcc acaataagcg
     2461 tagagaaaga caggatatgt tgtttggtgc agcacttaaa gcaggtcttc cggtaaccat
     2521 ctttgataga aactctgata gaaaatcgga aaattatcgt tacccaaaaa atagctttaa
     2581 tttaaatgtt caacctaatg ttagttatac tgaaactgct aatatttatc gtgattatct
     2641 tgtttctttg aatattaata ctgtcgaaga ttcccctacg atgttttctc gcagagtcgt
     2701 tgaaatatta gcttgtggag gaatattagt ctctacacca agtatggcta tcgacaatat
     2761 atttaaagaa tattgtcata tcgtacaaag tgaagatgag gctatagaat tattccaacg
     2821 tttaaaacga ggtgcttctc gtgatgattt gaataaagcg attgcaggct ctgaatttgt
     2881 attgagtaac tttacttggg aacatttctt atcccaa
//
LOCUS       DANLUA010000233         2916 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLUA010000233 DANLUA010000000
VERSION     DANLUA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            67..2106
                     /gene="malQ"
                     /locus_tag="PXB44_002445"
     CDS             67..2106
                     /gene="malQ"
                     /locus_tag="PXB44_002445"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5282873.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAF
                     QQSEQAQQWFHTKETQQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQT
                     AFDAFIEQAGEPLKIQATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQ
                     ATQIRFYMWLQFVANQQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2174..2905)
                     /locus_tag="PXB44_002446"
     CDS             complement(2174..2905)
                     /locus_tag="PXB44_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL5282874.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT          932 a          619 c          571 g          794 t
ORIGIN      
        1 ttcttactca tattaatctt cacaagatcg ttattttaac aaatacagta aaggaaaaaa
       61 gtcataatgt caaacaaaca aaatacagct cactattttt actccccaaa tattaaagag
      121 tatgccagcc cctctgcttt tgagaaaata gagcggcttt taaataccga taacgatttg
      181 caaaaaaata atcaaattct accgcttgtt aaagtgatta ctcagccaaa tgccgagcaa
      241 caagcggtcg aaatttcgct gaattttaca aatgtagcaa agtggcaaat tgtgcttgaa
      301 aatggtgagg ttcggaacgg tgaaattaag gcagagcaat cagttctaac cctgcctgcc
      361 gatttgcctt taggctatca tcagctttct atttcaactg aaaatgatga agaatcttgc
      421 tcgctgattg taacaccgcc gaaagctttc cagcctaagc cattggaaga aaggaaaaag
      481 ctctggggtg catttttaca gctttatact ttgcgttccg ataacaactg gggaattggc
      541 gattttggcg atctcaaaca attccttgcc aacatttctg attatggtgc agattttgtg
      601 ggcttaaacc ctattcacgc cctctttccg gcagatcctg atgcggcaag cccttacagc
      661 ccgtcctttc gactttggca aaatattatt tatattgatg tttcagcagt agaagccttt
      721 caacaatctg aacaggctca acaatggttt cacaccaaag aaactcaaca gcaacttgcc
      781 gaattgcgag ccaaagattt tgtcgattac agccaagtag cgcaactgaa attaaaggct
      841 ctgcagtttg cttttgagca tttttcacag tcatctcaac aacaatcgca aacagcgttc
      901 gatgccttta ttgagcaagc aggcgagcca ttaaaaatcc aagcaacgtt cgatgctttg
      961 caccactgga tttcatctga atttggcgaa aaaggcggct ggacaaactg ggcggaggaa
     1021 tatcaaagtt actattcgcc taaagtagct gaatttcaaa tatcgcaggc aacgcagata
     1081 cgtttttata tgtggttgca atttgtggca aaccagcaat tagcagaatg caaccggttt
     1141 gctcaatcac tttcaatgcc gattggtttt taccgcgatt tagccgttgg tgttactcaa
     1201 agcggagcgg aaacttgggc ggataaagcc ctatttgtgc aagaggcgtc aatcggtgca
     1261 ccacctgata tgcttgcccc gaacggacag agctggggac tttcgccaat gcacccgcat
     1321 attctacaaa gccgaggtta tcagccgttt attgatttag tgcgtgctaa tatgaaacat
     1381 tgtggggcat tgcgaatcga ccatattctc ggcttcgccc gtatgtggtg ggtggcgaac
     1441 ggtgaatcgg caaaagaagg gatttatgtc cgttatccgc tcgaagattt gctctctatt
     1501 ttggcattag aaagccaacg tcaccaatgt ttagtgattg cagaggcact tggcattgtg
     1561 cctgaaggca ttttggactc tctcgagcaa aagggcattt ttgcctacaa tgttttttat
     1621 tttgaaaagg cagaaaacgc gtttaaaccg ctggaaaatt atccttacca agcgatgaca
     1681 actctaagca cccacgattt accgactatt caaggctatt ggaaaggcta tgattttgag
     1741 ctaggcgaaa aatttgacac ttacccaagc aaagccgtat tgagaaagct gaaagaagag
     1801 cgttcgtacg accgcttgaa tatccgtaat gcggtggaaa aagtgatagc actcgagcca
     1861 aatgaagtgg gagtaacaat aaaatttacc caccaattac aacgctatgt ggctcacact
     1921 cacagtgcat tattcggtac acagcccgaa gattggctca atatgttaga gccggtgaac
     1981 attccgggaa ccagcaccga ataccctaac tggagaagaa aattatccgc aaccaccgag
     2041 cagatttttg caaataaaga tattcaacaa ctgctaaaag atataaacga aattcgtaaa
     2101 ggataataac aagcggtcat ttttaggtaa atttttacaa aaaatgaccg cttgtaattt
     2161 gtatttattc gctctattgc tgatgttttc tgtgaagttt aatccagttg taataaccgt
     2221 aaatagagtt acataaatac atcacataca tcaccaacag cggcaagctg gtttcgccgt
     2281 tttggaagta cattgccgtc cataaagaaa tggtcataat attcacgata atccacaatg
     2341 cccattgctc acggtagcga agtaccatca aaatttgtgc cacaatcgaa ataaccactg
     2401 ttgcaccatc taatatagct aacgcactgc ctaaatattt aagcaattca atataaagaa
     2461 atgtgccgat aatggtggta atggcgacaa tcacccactg ttttgcggtt aaagctttag
     2521 caattacctc ttccacacca gcattattta ccgtgttttc gctggtcata tttttacgcc
     2581 agaaatagaa accgataaac tgcaccggca catatacaag taaattcagc atcatttcac
     2641 catataactg aaaggtataa gagatataag cataaagcga aacggaaatt aagccgaata
     2701 gatagttact gatttttcct ttaccgacaa ataccacgca taaaatacct gttattgccg
     2761 caatagttgc aatccaagta tcgggttgat aaataaaaat accgatttga atcgctaaaa
     2821 aaacaatcaa ccaacttgcc tcaaaagtag tccaaccact aaaaaactct tgttttaact
     2881 tagttgaaaa agatgaacag cccatatttc ctccat
//
LOCUS       DANLUA010000234         2848 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLUA010000234 DANLUA010000000
VERSION     DANLUA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            157..1131
                     /locus_tag="PXB44_002447"
     CDS             157..1131
                     /locus_tag="PXB44_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5282875.1"
                     /translation="MNTQTKFDLYQHITDRIVAALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEYGYKQSKWITAKKANELGGYIRKGEKATVVMNYRPDK
                     REKLDEEGNIVYDEEGNPEIEQHFFIKKHNLFNLDQCENLPEELYDPISEPKQPTSDP
                     NQYEIFADIRQIIKGMDLHVEMKPSNRAYYQPSADLVVMPEIKQFENAQAFHSVLLHE
                     MTHATGAAKRLDREGITSGKAKFGNKVYAFEELVAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVIKEDKRAIVRASGKAREACQYMLDALNISQLADKNEVFEEAA"
     gene            1271..2017
                     /locus_tag="PXB44_002448"
     CDS             1271..2017
                     /locus_tag="PXB44_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282876.1"
                     /translation="MPNWCENKLQIFCDCIQAKQIIPKLFRKTESDKWCLDFELLVPM
                     PESICIESSSLGDRAMEFLKISPNRRLTRPLLKKYIYGSEIDRIYAKAKHHRWDVGHF
                     INWLIKRPEQQSILWLNLALGQQYLSNLAQHGYKDWYDWSNEQWGCKWNVCSDSCQVS
                     FNDNGSINCLFDTPWGPPDGWFQALCTAFPDIEFILSFFEPGMWFAGKYIANLDGSYC
                     NIYIDDRGIRSFAEEVFNEEFEDEFEEKLE"
     gene            2090..>2848
                     /locus_tag="PXB44_002449"
     CDS             2090..>2848
                     /locus_tag="PXB44_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5282877.1"
                     /translation="MENFEKQFISLFQRIVPHYRRSEVFYDFITLSALDMYLVTYRDQ
                     AEPNLRERFAHAKARYSDSEFTQLAELLAVTVNALTQKRYDFLGSVFMNLNLGDGYRG
                     QYFTPSHIADLMAKVTLQDCDRIISQQGFVTLSEPTCGSGVMVIGCVNAMFDAKYNPQ
                     QQLWVHCQDVDFTAAMMCYIQLSLLHIPACIVVGDTLLNETKIQMYTLAHLMGNWSHK
                     LEQQIAPLVNSTTEKGCGNNIEKSVIVEDLSQISI"
BASE COUNT          903 a          500 c          587 g          858 t
ORIGIN      
        1 tatctgtagt tactgcatta taggcagttc tactggtttt gatgaaccta gaaaaactat
       61 ctctgttgag attttaaacc acccatcggg gaaactagtg cccgataagg gtaatagtgt
      121 ttccccattt tctacctgaa ctttggagaa acactaatga acactcaaac taaatttgat
      181 ttataccaac atatcacaga ccgaattgtt gcagcactgg aacaggggac tgcaccatgg
      241 ttaaaaccgt ggagtcatcc agaatgtaat atggcattac ctcgtaatgc ggtctctaac
      301 cgattgtatt ctggtatcaa cacactatta ctctggattg ctaccgcaga atacggttac
      361 aaacagagta agtggattac agcaaaaaaa gctaacgagc tcggcggata tatccgtaaa
      421 ggtgaaaaag ctactgttgt aatgaactac cgtcctgata agagagaaaa acttgatgag
      481 gagggaaata ttgtgtatga cgaggaaggt aatccagaaa ttgaacaaca ctttttcatt
      541 aagaagcaca atctgttcaa tctagaccag tgtgaaaatt tacccgagga actgtacgat
      601 cctatatcag aacctaaaca gcctacatcg gatccaaacc aatacgagat ttttgctgat
      661 attcggcaga ttatcaaagg aatggattta cacgtggaaa tgaaaccgag taatcgggca
      721 tattatcaac ccagtgcaga tttagttgta atgccggaaa taaaacagtt tgaaaatgct
      781 caagcgtttc atagtgtgtt attgcacgaa atgactcatg caactggtgc tgctaaaagg
      841 ctcgatcgtg agggtattac ctccggaaaa gcaaaatttg gaaacaaagt ctatgctttt
      901 gaggagttgg ttgccgaaat ggggggagca tttttgtgtg cctaccttgg atttgatacg
      961 gtacctcaaa atgcggcata tattgaaagt tggataagag ttataaaaga agataaacgg
     1021 gctattgtcc gggcttctgg taaagctcgt gaagcttgtc aatatatgtt agatgcatta
     1081 aatatctcac agctggctga taaaaacgag gtctttgaag aagcagctta actctttgct
     1141 gaaaggtact ttcaacacaa tctttcatag taatacataa tcctttagga attatgtatc
     1201 gattatcttt taacccttgg ggtacattgc ctcaaggcaa gtgtacccct actttattta
     1261 ggagtacatt atgccaaact ggtgtgaaaa taaactacaa attttttgcg attgtatcca
     1321 agctaaacaa attattccta agcttttcag aaaaactgaa agtgataaat ggtgtttaga
     1381 ctttgaatta ctcgttccaa tgccagaatc aatctgcatt gaatcaagta gtcttggtga
     1441 tagagcaatg gaatttctaa aaatttcacc aaatagacgt ttaacacgcc ctctactcaa
     1501 aaagtatatc tatgggtccg aaattgatcg gatatatgct aaggcaaagc atcatcgctg
     1561 ggatgtaggt cattttatca actggttgat aaaacgtcct gaacaacaaa gtatattatg
     1621 gctaaacctc gcattaggcc agcaatatct gtctaaccta gctcaacatg gttacaaaga
     1681 ttggtatgac tggtcaaatg aacagtgggg ctgtaaatgg aatgtatgtt cagatagctg
     1741 tcaagttagc tttaatgata atggatctat taattgccta tttgatacgc catggggacc
     1801 acctgatggt tggttccaag cactttgtac tgcctttcct gacattgagt ttatactttc
     1861 attttttgag ccgggaatgt ggtttgcggg caaatatatc gcaaatcttg atggctcata
     1921 ctgcaatatt tacatagatg ataggggtat tcgcagtttt gctgaggaag tctttaatga
     1981 agaatttgaa gatgagttcg aagagaaact tgaatagcct taattaattt ttccacccta
     2041 ggggtaattc cctaggggga ttactccgta aactttaagg agtagtctaa tggaaaactt
     2101 tgaaaagcaa tttatcagct tatttcaacg tatcgtacca cattatcgac gttcagaagt
     2161 gttttatgat tttattacac tctctgcact tgatatgtac ctagtaacgt atcgagatca
     2221 agcagaacct aatttgcgag aacgctttgc tcatgctaag gcacgatatt cagattctga
     2281 gtttactcaa ttagcagaat tgttagctgt tacggtgaat gctttaacac aaaaacgcta
     2341 tgatttctta ggctcagtat ttatgaatct caatcttggt gatggttata gagggcaata
     2401 ctttacacca tctcacatcg cagatctaat ggcgaaagtt acactacagg actgtgatag
     2461 aatcatctca caacagggat ttgttacttt atcagaacca acttgtggtt cgggtgtaat
     2521 ggtgattggt tgtgttaatg cgatgtttga tgcgaaatac aatccccaac aacaactttg
     2581 ggttcattgt caagatgttg attttacagc tgccatgatg tgttatatcc agctatcgtt
     2641 attacatatt cctgcttgta ttgtagttgg tgatacgcta ttgaatgaaa caaaaattca
     2701 aatgtatacg ctggctcatt tgatgggaaa ttggtctcat aaactggagc aacaaattgc
     2761 accacttgta aatagcacta ctgagaaagg ctgtggtaac aatatagaaa aatcagtgat
     2821 agtggaagat ttatctcaaa tctcaatt
//
LOCUS       DANLUA010000235         2838 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLUA010000235 DANLUA010000000
VERSION     DANLUA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            58..1539
                     /gene="ilvC"
                     /locus_tag="PXB44_002450"
     CDS             58..1539
                     /gene="ilvC"
                     /locus_tag="PXB44_002450"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL5282878.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
     gene            complement(1680..2537)
                     /locus_tag="PXB44_002451"
     CDS             complement(1680..2537)
                     /locus_tag="PXB44_002451"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL5282879.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
BASE COUNT          816 a          554 c          650 g          818 t
ORIGIN      
        1 aacaatgtgc aaacaaaaca tcatatcaat ctcattaatt caaataagga ttaaaaaatg
       61 gctaactatt tcaacacttt aaatttacgt caaaaattag accagttagg tcgttgccgc
      121 tttatggatc gtgatgaatt tgcagatggc tgcaatttct taaaaggcaa aaaaatcgtg
      181 attgtgggtt gtggtgctca aggtttaaac caaggtttaa atatgcgtga ttcaggctta
      241 gatattgctt acgctttacg tgccgaagcg attgctgaaa aacgtgcttc atttaaacgt
      301 gcttctgaaa acggctttgt ggtgggtact tacgagcaat taattcctga agcggattta
      361 gtcattaact taacgccgga caaacagcac tctaaagtag ttgccgatgt aatgccgtta
      421 atgaaacaag gtgcggcatt tggttattca cacggtttca acattgtgga agaaggtgag
      481 caaattcgtc cggatttaac ggttgtgatg accgctccga aatgcccggg cacggaagtg
      541 cgtgaagagt acaaacgtgg tttcggtgtg ccaaccttaa ttgccgttca cccggaaaat
      601 gaccctaaag gcgaaggttt agccattgcg aaagcgtggg caagtgctac cggtggtgac
      661 cgtgcgggcg tattagaatc ttcattcgta gcagaagtga aatctgattt aatgggtgaa
      721 caaaccattc tttgcggtat gttacaagca ggttcaatcg tgtgctacga caaattagtg
      781 tcggaaggta aagatccggc ttacgcaggt aaattgatcc aatacggttg ggaaaccatt
      841 accgaagcgt taaaacaagg cggtatcacc ttaatgatgg atcgcttatc aaacagtgcc
      901 aaattgcgtg cgttcgagct ttctgagcaa atcaaagaag aattagcgtt cttatttgaa
      961 aaacatatgg acgacatcat cagtggtgag ttctcatcaa cgatgatggc agactgggca
     1021 aacggcgatg ccaacttatt aaaatggcgt gaagaaacag gtaaaacagc ctttgaaaac
     1081 gcaccacaag cagacggtat taaaatttct gagcaagaat actttgataa cggtgtgtta
     1141 atggttgcaa tggtgaaagc aggggttgaa ttagcctttg atacaatggt ttcagccggt
     1201 atttatgaag aatcggctta ctacgagtct ctacacgagt taccgttaat cgcaaataca
     1261 atcgctcgta agcgtttata tgaaatgaac gtggtaattt cagataccgc agaatacggt
     1321 aactacttat tctctcacgt tgcaaccccg attttagcgg agaaaattat cccaacgtta
     1381 gagaaaggcg atttaggtga accaactccg gcagtggaaa tcgacaacgt taccttacgt
     1441 gatgtgaatg acgcaatccg taaccaccca atcgagttaa tcggtcaaga attacgtggt
     1501 tatatgacag atatgaaacg tatttcttcg caaggctaac aagcggtcag atttacacaa
     1561 aattttgcaa aagtgcggta gaataaccgc actttttacc actaactaaa aaacctgtaa
     1621 gtgttggctt acaggttttt gttttgcaaa aaatagcgta aatttgaccg cttgtggtgt
     1681 tatttggtta ataaatccaa tgcttcttga tatttcgcta cggttttttc aattacctct
     1741 gccggtattt tcggggctgg gggttgtttg ttccaaccgc tattttccag ccagtcacgc
     1801 acgaattgtt tatcaaatga aggtggattt gtgccttctc ggtaggtttc aactgaccaa
     1861 aaacggctgg aatccggggt taggacttcg tccattagcg ttagttcacc gttttcgtct
     1921 aagccgaatt cgaatttggt gtcgcaaata ataattcctt tggtcagggc atagtttgcc
     1981 gcttcagtgt agagttttag agcggcatca cgcactttgg cggcaagttc ttgaccgatc
     2041 tggcgttcac actcttcgta gctgatatta atatcgtgat cgcccacctc ggctttgctg
     2101 gacggtgtga agatcggttg cggtaatttg ctggcctcaa ccaagccttc cggcaatgtt
     2161 aagccgcaga tcgtgccggt ttgttggtaa tctttcagcc cggagccggt taaataaccc
     2221 cgcacaatcg attcaatttt caccggtatt aagcgtttgc aaaccaccgc acgatgttga
     2281 atagcttctg cctcttcttg cggtaacacg tcaaacacac tgtcgccggt gaagtggttt
     2341 ggcataatgt gggctaattt gttgaaccag aagttggaga tttgggtgag gatttcgcct
     2401 ttacgaggaa taggatcgtc taaaataaca tcaaaggcag ataatcggtc ggtggcaacc
     2461 ataagcatac gtttttcatc gatttcgtag agatcacgga ctttgccgga gtagatcttt
     2521 ttgaggctga tttgcatagt gttattccta ctttgttgaa tttgcaaaaa atcaggcaaa
     2581 ttataccgct ttacgcaaac gtttgcgata gttgttttag agagatgaaa aatagcaatt
     2641 ttttcgcaaa aaacacttgt cattgccaaa atttaagcgt atcttttaaa gcaatttagt
     2701 ttggagaaca acaaaatggc ttttattcgt actcatcacc accatcattt acctgaataa
     2761 tctttcgggc atttggtatg tgcggaagat aagacttccg agtacgccta aacgcagcat
     2821 aaatttaacc ctctcgga
//
LOCUS       DANLUA010000236         2830 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLUA010000236 DANLUA010000000
VERSION     DANLUA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..529
                     /locus_tag="PXB44_002452"
     CDS             <1..529
                     /locus_tag="PXB44_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282880.1"
                     /translation="KYILPLIAFTALILFLAIVFPENRYVEHALEPVINLLSGTGKVS
                     SSTDNLVKNHLFLPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACFA
                     FTYYFVRKVAVNWFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTSG
                     RNVTFFCKNKEAEYV"
     gene            522..1487
                     /locus_tag="PXB44_002453"
     CDS             522..1487
                     /locus_tag="PXB44_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5282881.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            1487..2287
                     /locus_tag="PXB44_002454"
     CDS             1487..2287
                     /locus_tag="PXB44_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5282882.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(2324..2830)
                     /gene="msrA"
                     /locus_tag="PXB44_002455"
     CDS             complement(2324..2830)
                     /gene="msrA"
                     /locus_tag="PXB44_002455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5282883.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
BASE COUNT          838 a          499 c          594 g          899 t
ORIGIN      
        1 gaaatatata ttgccactga ttgccttcac tgcgttaatt ctatttttgg cgattgtttt
       61 tccggaaaac cgttatgttg aacacgcctt agaaccggta attaatttat tgtccggcac
      121 cggaaaagtc agctcttcaa ccgataattt agtgaaaaac catctgtttt taccgacttt
      181 aaatcagttt atttccggtg atggtttata ttacaccgaa actggtggct attatggtgg
      241 atcggattcc ggctatattc gccaagtatt atatggtgga gtaagttata tgttggcgtg
      301 ttttgccttc acttactatt ttgtacgcaa agtggcggtg aattggtttg aaggcagttg
      361 gatttttacc ttatccacca tcggcatttt agctatttta aatattaaag cagatacctt
      421 tgctttcccg gggctgatga tgctgtttct aatgttcttg tcgctgtttg gaacaagcgg
      481 tcgaaatgta acattttttt gcaaaaacaa agaggctgaa tatgtttagt attattgtgc
      541 cttcttataa ccgtaatcaa gaaatcaatg cgttgcttga gagtttaaag caacaaactg
      601 catataattt tgaagtgatc attgttgatg actgctcaaa aataccggtc agtgaaactt
      661 taggaatttt aaatgaattt aacgatattt ctactaaaga aaaagtaaga gaattactta
      721 ctagagatag tttatctcat atttatagct atccatttga tgtgaaagtg gtgcgaaata
      781 acccaaatgt tggggcggca caaagccgaa atgtaggggc aactaatgcc acacaagaat
      841 ggctactgtt tttagatgat gatgaccgct ttatgcctga aaaatgcgaa cgtttagcgg
      901 cagagattgc gaacaatcca actgccaatt ttgtgtacca cccggcaaaa tgtgaaatgg
      961 taaatgaggg ttttacttat gtcaccaagc cgtttaaaca taaaagtgag ctaactaaag
     1021 aaaacattct aaaagcgaat aaaattggcg gaatgccgat gattggcttg aaaaaatcac
     1081 tcttttgtaa aattggtgga ttatctgacc gcttgcgatc acttgaagat tatgatttct
     1141 tactgaaatt attggaagaa ccgagttttg agccattgta tgtggacgaa gccttgacct
     1201 attgtacttt ccataccaaa cgctccagtg tttcaaccga taccactaat acagaaaagg
     1261 ctattgagta tattcaacaa acgtatatca aaacacctga ggcgaataat tttaagctca
     1321 attcgcttta tattttaagt tatccacacg taatgaattt atcccgcaag gcagccggtt
     1381 attattttga gatgtttaaa cagactaaaa atatcaaaca actgattatt gcgttggtta
     1441 tttttatttc gccgaagttg gcgattaatt tgaaaaggtt tatgtaatga aattttccgt
     1501 tttaatgtcg ttatacatta aagagcaacc gcaatattta agagaatgtt ttgaaagctt
     1561 gaaagtacaa actgtgcagg ctgatgaaat cgttgtttgt tttgatggtg cagttacgcc
     1621 tgaattagag gcgattgtag aagaatatag tgcaattttg ccgattaatg cggtgaaatt
     1681 cccacaaaat cgaggtttag gtaaaacgct aaatgatggg ctaacctatt gtaagaatga
     1741 atgggttttc agaatggata cagacgatat ttgtatgccg gaacgtttcg agaaacaagt
     1801 ggcatttatt gaacagaatc ctgaaaccgt gatttttggc ggccaaattg ctgaatttgg
     1861 gcaagatatt cacgatcttg tcgcttaccg caatgtacca accacggtgg aggaaatcgt
     1921 cagattcact cgcttgcgtt gcccgtttaa ccatatgacg gttgcttatc agaaatccaa
     1981 agtgttggaa gtcggtggct atcaagatct gcaagaagat tactatttat ggatcaaact
     2041 cattgctcaa tttaaacaag tggcaaactt gccggattta ttagtgtatg cccgagtcgg
     2101 taacggaatg gttggcagac gccgtggaat ggcccaagca gaggcagaat ggcgtttata
     2161 taaattaaaa aaccgctgcc agattcacga tccgatttca ggttttggga ttttcataat
     2221 gcgtgcaatt cctcgtttat taccggtttc agcactgaaa aaagtgtacg gtttgctcag
     2281 aaaataaagc taaaaaaacc acagattgct ctgtggtttt ttgttattga ttcttcaaaa
     2341 tctcattcat taattgaata tcaatatggc agtagccgtt cgggtttttt actaaatatt
     2401 cttggtggta ctcttcggca gggaaatatt gctctaacat ttggttttcc actgccagcg
     2461 gctgatcaaa ttgggtttgt aattgtgcaa gtgcggcggt taccaccggt tcgtcttgtg
     2521 ggtcggtata ataaataccg gtgcggtatt gaataccgat gtcgccacct tgtttgttaa
     2581 tgctcaccgg gtctattact ttaaagaagt aatctagtaa ttttgccaag ctaatttgat
     2641 tggcatcata agtcactttt accacttcag cgtgtccgct accggcacac acctcttgat
     2701 agcttgggtt atcggtatta ccgttggcat agcctgattg tgccttaatg acacctttaa
     2761 tccgttgcat aaaagattcc acgccccaga aacagccacc ggctaagtaa atttcacgaa
     2821 tattttgcat
//
LOCUS       DANLUA010000237         2790 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLUA010000237 DANLUA010000000
VERSION     DANLUA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..169
                     /locus_tag="PXB44_002456"
     CDS             <1..169
                     /locus_tag="PXB44_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282884.1"
                     /translation="KGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERKAREL
                     ANFEQENIIYG"
     gene            162..449
                     /locus_tag="PXB44_002457"
     CDS             162..449
                     /locus_tag="PXB44_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282885.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(575..2677)
                     /locus_tag="PXB44_002458"
     CDS             complement(575..2677)
                     /locus_tag="PXB44_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5282886.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
BASE COUNT          812 a          551 c          518 g          909 t
ORIGIN      
        1 gaaaggctat gatgaattcc tagcagaaaa aattcgcctt ggcttagaag atatgcaatc
       61 aggtaatggt ctttcactcg atgaatcaaa agcacgaacc aaacaactga ttgaacgtaa
      121 agcaagagaa ctcgcaaact ttgaacaaga gaatatcatt tatggctaaa acattacgaa
      181 ttagcagtag agctcaaaat gatattgaag aaattttagc gagcgtaatt gaatatactg
      241 gttttgaaag tagtggaatt cgcttacaag aagatattta ccaaaaattt gagactatcg
      301 cttatatgcc ctctgcggct ggacgtttaa gagaagatgg cacaagagaa gcattcacta
      361 ggcgttatcg tattgtttat accgaattag aaaatgaagt gtggattatt accattattc
      421 atagtagcag actttatcca cgcatttaaa taacaaacga ctaaaatagt cggtttttta
      481 tttgcaaaaa attaataaaa tatcaccgct tgtaagctcc ataaaaaaaa gcccgattgt
      541 aacatcgggc gttttagtct gaatttaagt cagattagaa tgtgtagttc acacctgcac
      601 ggaattcacg tccacgagct gataattgac cacgctgacc gtgtgcgttg tattttttat
      661 tcgccacgtt atccactgcg aagtttacat tcacgctatc atctttaagt ggtttccagt
      721 ttaaggtgat atcagttaca ttgtagccgt ctttgcctgt accggtattt gtttgtaatg
      781 ttccgcccgt aacgaagaaa ttatcttcag gtttcacttt ctcaacaata cggtggtgta
      841 cgccaacctc taaatttggt tgttcaaagc gatagcttaa agaagctgtc caagtacgac
      901 cgatagctga ggcgtattct gggttagtac ttaataatgg tttttcttcc cgcttaccag
      961 tttgtctgtt tacttcccaa atagttggtg agtagaaacg cggtttgctg tgtgcaacac
     1021 caatacgagc agtaaaacca ccattatggt aacctgcatt taactcataa ccacggtttt
     1081 taattttacc taggttcata attaactcat tattaggatt atggctatca cgaccaccac
     1141 ctaatgcatc ttttatatct tgccagaaat agctaccatc aaaactgaat gtaccattgt
     1201 tatagttgaa accgatttca gtattacgag ctttctccgc tttagtattg ctaccaatac
     1261 taacgatttt tctatttccg tgagccgtta gtgcatcgta taaacgaggg ctacgagttg
     1321 catagttgtg gcttgcactt aagcttaaat gttcaattgg ctcatagata agcccaacgc
     1381 ttgggctgaa tgcaccatta ctacgctctt taccatccat tgctttgaat ttgaagtggt
     1441 cgtaacgtac accggtagtt aaagttactt tatctactgg agcagtaatt gcttcaacat
     1501 acacgccggt atcggttttc tcttggtttt tcacaagagg gttaaattta gtgtgtggtt
     1561 tagcttcttg gtggcgatag ttcacaccgt attttaataa tacattttcg tgaagggcag
     1621 aatcaaagtt tacgtttgca ccgtgggtta ctattttagt tttggttgca taatctacac
     1681 caccagcata accattacct gtttcattct cagaccaacg accttgtact aactgataca
     1741 cgttagctgt tgcttcttga gcaaaaccta agtttttacc agtccattct aagttagttc
     1801 tttgaaccgt aattctacgg ttagctggag cttggcgttc tacagttaag cggttgtacg
     1861 gagaaacctt tgtgccgtta ggtgctacat caaattcttc acgcactaaa cgctgacctt
     1921 catttcgctc acttgaatgg cttaatacaa aacggtgatc tcccaatgtt gcacctaatt
     1981 tagctaaaaa gctggtttta tctagtgcac tatacggaac acggtttgtg ccgaattggt
     2041 ttacgtaatc tttaccgcct ttatagtcgc gatctctcac aagattacct gagactaaag
     2101 catcaaagat ttcgccttta ccatataagg ttgcattgaa attgtgagcg tggtttgtgc
     2161 tgaagccagt acctaatttt gcaccgaagt ttttatcgct gttttttaat aaatctaatg
     2221 catcaacggt tttagcaatt actgcaccat tagtttgacc aataccggct gaagcactgc
     2281 ctgcaccttt ttgtacagaa acaattttca ccaatgccgg atctaacata tgacgacctt
     2341 ggtgatacaa aatttggcta tctgagtaag tgttatctac ttttacatca attgagtttt
     2401 gacccatacc acgaatatag agatattgtg atgtaccgtt accaccacct aagctaattg
     2461 ccggctcatc ttttaataaa ccacgcaaat cggtttgggt gctttcatct tttttcttag
     2521 ttgttaccac attgctttgt acttttaaac cttgattatc tgttacggta acagtatcta
     2581 atactactgc atctgttgaa gcagtttgag ccactgcatt agcagcaagt acgctcaata
     2641 ccgcagcaga aacagggagc aatttgaagc ttttcatttt atttatattc cttaggtaaa
     2701 ttatttaaga ggattatctg tttgatatta aaaataattc tcaacaccaa acgttcgcaa
     2761 attttacaga gataaaaatg attgtcaatt
//
LOCUS       DANLUA010000238         2688 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLUA010000238 DANLUA010000000
VERSION     DANLUA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1105
                     /locus_tag="PXB44_002459"
     CDS             <1..1105
                     /locus_tag="PXB44_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5282887.1"
                     /translation="VALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTNSKELD
                     NHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGFGDLYT
                     KRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIRYNYRG
                     VENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYKTATDS
                     TKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAFYVSPN
                     FEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVAFVEGS
                     YTRTKDYDKVTNGYEYSGKSTNKAIGVGMRVYW"
     gene            complement(1258..2622)
                     /gene="glmU"
                     /locus_tag="PXB44_002460"
     CDS             complement(1258..2622)
                     /gene="glmU"
                     /locus_tag="PXB44_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL5282888.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          763 a          503 c          536 g          886 t
ORIGIN      
        1 agttgcatta gcagtaaccg cgttcgcagc gtcagcatca gcaaccaccg tttacgatgc
       61 agaaggttca aaagtagatt ttgatggttc tttacgtatt gttcttgata gaacaaattc
      121 taaagagcta gacaatcata atgttgttaa aactaaaaaa gatgcacaca caggaatccg
      181 taacactggt tcacgttttg gtgtaagagt aaaacatgat ttagctcaag atttctatgc
      241 attaggtcgt gttgagttcc gttttaacaa aaataccagt gatgatggtt ttggtgattt
      301 atatactaaa cgtgcttatg ttggtttagg tagtaagcaa tatggtgaat taacttttgg
      361 tcgtcaagta acgattgctg atgatttaag tcaatcagta gattacaact acggtattaa
      421 tccaaaaggt gattatattc aaaccgatgg taaccaagtt attcgttata actaccgtgg
      481 tgttgaaaac ttacatgtaa gtgcaaacta taactttgca caagatcgta aagatggtaa
      541 agtattagca aataaacttc aaaatgcata cggcattggt gcattataca ctgtagataa
      601 ctgggattta cgtgcagctt atggtcatac aaactacaaa acagctactg atagcactaa
      661 gcaccgtaaa gatggtgtat taggttctgt tggttataaa tttggtgact taaaattaag
      721 tgttgataca ggttattctc gtgagaaggg tttagctgtt acggatgata atggtgtaac
      781 tacattctat aatggtgtta aatcaaacgc tttctatgta tctccaaact ttgaatatca
      841 agtagtgcct gcatctaaag tatatggtaa ctacttatat gagcgtgtga aatctaaatc
      901 aaatggttct acatatgctg ctggtaaaga aaaaactcat ggtttcttac ttggtgttga
      961 ttaccaattc cataaacaag tagttgcttt tgttgagggt tcttatactc gtactaaaga
     1021 ctatgataaa gtaactaatg gttatgaata ctcaggcaaa tcaactaaca aagctatcgg
     1081 tgttggtatg cgtgtatact ggtaatcaat tgattttcta aattgataaa cccctaacaa
     1141 tatttattgt taggggtttt gttttaaata tattgttcat tatccttttt aaagtgtatg
     1201 gctttgctgt tattggcaaa gccaattttt aaatataata acttttgacc actggcacta
     1261 tttcttttta gtcgggcgtt gccagccttg aatatggcgt tgtggtacac ggctaattac
     1321 cagttcattt tccgcaatgt ctttagtgac tgttgcaccg gcaccaatgg ttgaaccgct
     1381 ggcaatcgtg accggggcaa tgagttggct gtctgagccg acaaatacgt tatcgccaat
     1441 aatggttttg aatttattcg caccatcata gttacaagtg attacacctg ctccgatgtt
     1501 acattctttg ccgatttccg catcgcctac ataagtgaga tgatttactt ttgaaccttt
     1561 gccgatggta gcttttttga tttccacaaa gttaccgata tgggtttctt ccgctaattc
     1621 tgcccccggg cgtaagcgag agaatggacc gatagcggat tttgccccga caatcgaatc
     1681 ttcaatcaca gaataaggtt taatttcaac attatcgcca atttcgcagt ttttcagtac
     1741 gcagcctgca ccaattttta cattgttacc gagtttgatt tcaccttcta gaatgacatt
     1801 cacatcgatc tgaacatctt ttccgtgggt gacagtgcca cgaagatcaa aacggcttgg
     1861 gtcgataata gttacaccag ccaataacag tttttccgcc tcacgttttt ggtagaaccg
     1921 ttcaagtgca gcaagttgca aacggttgtt tgccccttct acttccataa attcagttgc
     1981 ctgaaccgct tgtactttaa agccttcttg gtttgccatt gcgatcacat cagtgatgta
     2041 atattcacct tgagcattgt tgttatttag gtttttcaac cattttttga agctcgcacc
     2101 gcttgctacc atcacgccgg tattcacttc tttgacttta agctgtgctt cattggcatc
     2161 tttttgctca acaatcgcta ccacagaacc attttcacga ataattcgtc cgtagcctgt
     2221 cgggttttcc aattcaacgg ttagtaatgc aataccattt tccggtttgg ctgcgattaa
     2281 acgctctaaa gtggcttttg tgattagggg ggcatcacca taaagcatta caatgttttc
     2341 atcatcagcg aaaaaaggag ctgcttgttg cattgcatgt cctgtgccta attgttcggc
     2401 ttggaatacc cagtttacag gctcttcgct taaacgtgct tttaacagat cggcaccgtg
     2461 tccgtaaatt aaatgaattt gctgggcatc tagctgtttg gcagtatcaa taacgtgttt
     2521 taccatgggt ttaccggcaa ctgggtgtaa cactttgggt aaatctgaat acatacgagt
     2581 gcctttaccg gcagctaaaa taacaacgct cagttgagtc atatagaatc cttatttgac
     2641 taaatagatg aaggcttata gtaacgtgta aattgctata agtgatat
//
LOCUS       DANLUA010000239         2592 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLUA010000239 DANLUA010000000
VERSION     DANLUA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            152..442
                     /locus_tag="PXB44_002461"
     CDS             152..442
                     /locus_tag="PXB44_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL5282889.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            511..2151
                     /gene="groL"
                     /locus_tag="PXB44_002462"
     CDS             511..2151
                     /gene="groL"
                     /locus_tag="PXB44_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL5282890.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2276..2590)
                     /locus_tag="PXB44_002463"
     CDS             complement(2276..2590)
                     /locus_tag="PXB44_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL5282891.1"
                     /translation="MLGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECEKMFN
                     TANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNSLKQK
                     HQ"
BASE COUNT          824 a          421 c          619 g          728 t
ORIGIN      
        1 atatagtttt atcaaaaatg gtcaaatttg taaaaaattt tacaaatttg accgcttgca
       61 cttgaaactc taaaatctgc ctttatattg ttaggcgtta cgcattttgc gtgaagtttt
      121 atttattcat ttttaagaaa aggaacatat tatggcactt cgtccattac acgataaagt
      181 tattttaaaa cgtgaagaag ttgaaacaaa atcggcaggc ggtattgttt taaccggttc
      241 ggcagcaaca aaatcaaccc gtggcaaagt aattgcagta ggaacaggtc gcattttaga
      301 caatggttct gttcagcctt tagcagttaa agtgggcgat gtagtgattt ttaacgaagg
      361 ctacggcgta aaaaccgaga aaattgacgg cgaagaagtg ctaattttag cagagcacga
      421 tattttggcg attgtagaat aatttcgttc tgcacaagcg gttaaaaaag caaaattttt
      481 tacatatttc aaatacaaag gatttagaaa atggcagcaa aagacgtgaa atttggtaac
      541 gatgcacgcg taaaaatgtt aaaaggcgtg aatattttag ccgatgcggt aaaagtaact
      601 ttaggtccga aaggtcgtaa tgtggtgtta gatcgtgctt atggtgcacc aacgattaca
      661 aaagacggtg tgtcggttgc ccgtgaaatc gaattagaag ataaatttga aaatatgggg
      721 gctcaaatgg tgaaagaggt tgcctctaaa gccaatgatg ccgccggtga cggtacaact
      781 accgcaactg tattagctca agcgattgta aacgagggct taaaagcagt ggctgcgggt
      841 atgaacccaa tggatttaaa acgtggtatc gataaagcgg ttgcagcagt ggttgaagag
      901 ttaaaagcga tttcaaaacc gtgcgaaacc tcaaaagaaa ttgagcaagt agggacgatt
      961 tctgcaaact cagattcaac agtcggtcaa ttaattgctc aagcaatgga aaaagtgggc
     1021 aaagagggcg tgattactgt tgaagacggt acaggtttag aagatgcatt agacgtggtt
     1081 gagggtatgc aatttgaccg tggttactta tcgccatact ttatcaataa accggaagcc
     1141 ggtacggttg agttagaaaa cccatatatt cttttagtgg ataaaaaagt gtctaacatt
     1201 cgtgaattat taccggtgtt agaaggtgtt gcaaaagcag gtaaaccgtt attaattatt
     1261 gccgaagata ttgaaggcga ggcgttagct accttagtgg tgaacactat gcgtggtatc
     1321 gtgaaagtgg cggcagtgaa agcacctggc tttggtgatc gccgtaaagc aatgttacaa
     1381 gatattgcga tcttaactgc aggtactgtg atttctgaag agatcggtat ggagcttgaa
     1441 aaagcaaccc ttgaagagct tggtcaagca aaacgtgtgg ttatcagcaa agataacacc
     1501 acaattattg acggtattgg tgatgaaact caaatcaaag gtcgtgtagc acaaatccgc
     1561 caacaaatcg aagattcaac ttcagattac gacaaagaaa aactacaaga gcgtgtagca
     1621 aaattagccg gtggtgttgc ggtaattaaa gtaggtgcgg caactgaagt ggctatgaaa
     1681 gagaaaaaag accgtgttga tgatgctctt cacgcaaccc gtgctgcggt ggaagaaggt
     1741 attgtaccgg ggggcggagt tgctttagtg cgtgcagcca acaaagtggc tgatacacta
     1801 aaaggcgata acgaagagca aaatgtaggt atcaaacttg ccttgcgtgc aatggaggct
     1861 ccattacgcc aaatcgttgc caatgcaggt gaagaagcct cagttgtagc ccgcaacgtg
     1921 aaagaaggtt caggcaacta cggctataac gcaggtacag agcagtacgg cgatatgctt
     1981 gaaatgggaa ttttagaccc aactaaagtg actcgttcag cacttcaatt cgcggcttct
     2041 attgcaggtt taatgatcac tacagaatgt atgattaccg atctgccaaa agaagagaaa
     2101 ttagatcctg ccgcaatggg cggtatgggg ggaatgggcg gaatgatgta attgcccatt
     2161 gagtgaacaa aagctactga aattaattca gtagcttttt tgttggtaac aagtggttaa
     2221 atttatgcta ttttttgcaa ataaaaaacc aagcaagtaa gcttggtttt agcgtttatt
     2281 gatgtttttg tttaaggctg tttagtgctg ttaaaccttt ctcacaactt ttttgctgta
     2341 attcagaatc catttttagg atttgttgct ttgttaaaga aagtttattt ttcattttta
     2401 gcacttgagt atgagtacca ggctgtcttt ctgcatcaga gaccaattta tttgccgtgt
     2461 taaacatttt ctcacattca gttggcaaat ttttacttaa agtaggtttt gcaactgtaa
     2521 gtgatgaggt tttaacactt gctatagcat tactggaaac caatatggta caaagtgtta
     2581 ttcctaaaat tc
//
LOCUS       DANLUA010000240         2577 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLUA010000240 DANLUA010000000
VERSION     DANLUA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            54..200
                     /locus_tag="PXB44_002464"
     CDS             54..200
                     /locus_tag="PXB44_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282892.1"
                     /translation="MLAEKPKKRWRFWYAFVNGLFFDKKHCYAAYKAEVYRKQYPPEF
                     TEIT"
     gene            307..1488
                     /locus_tag="PXB44_002465"
     CDS             307..1488
                     /locus_tag="PXB44_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDL5282893.1"
                     /translation="MSILDTYLHGVEVVEVNAGGVTISTAATSVIGVVCTGDQADAET
                     FPLNTPVLITNPLNYLEKAGSTGTLRRTLNSIGSIVKTPTVIVRVAESDDSDTLTANI
                     VGTQENGKFTGIKALLTAQSTVFVKPKLLCVPQHDNQAVATELLSVAKKLNAFAFISD
                     NGATTKEQAYTYRQNFSQREGMMIFGDWKSYNTDKKAYDTDYAVARACALQAYIDKTV
                     GWHKNISNVELDGVTGITKAVEFDINESSTEANYLNEKGITICLNHNGFRYWGSRTLA
                     TDTRWAFQQSVRTAQIIKETIGAGLAWAVDMPLTPLRVKTMLEAINNKLRSWASGDDP
                     RILGARVWVAEEITADIIKSGKFVIKYDYHWIPSLESLGLEQRVNDEYVVDLVNTLKA
                     L"
     gene            1497..2003
                     /locus_tag="PXB44_002466"
     CDS             1497..2003
                     /locus_tag="PXB44_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5282894.1"
                     /translation="MGLPAKLKNFNFSVDGTSYLGETTEVTQPKLAMQLEDYRAGGMI
                     APVGVNMGLEKLELEFKIGGHEEDLLKLFGGSISGNAFRFNGAYQHDDDDSVDAVELV
                     CRGRIAEIDEGSSKAGDDTEHSYKASLTYYKKTVNGVDIIEIDTLNQIYIVDGKDRLA
                     EIRKAMGL"
     gene            2082..2396
                     /locus_tag="PXB44_002467"
     CDS             2082..2396
                     /locus_tag="PXB44_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5282895.1"
                     /translation="MKKVTLKQGILRGEKRITEIEVRKPLTKQLRGTNLTSLMQLSVD
                     EWCIVLPRITTPKLDKADFATMSAADLLKLSGKALDLMSEDFDEADEEDSEEQGKGEI
                     LA"
     gene            2420..2536
                     /locus_tag="PXB44_002468"
     CDS             2420..2536
                     /locus_tag="PXB44_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5282896.1"
                     /translation="MADIATVFHWTPNTFDEMTIVELGQWREKARLRNGVES"
BASE COUNT          761 a          564 c          604 g          648 t
ORIGIN      
        1 caatttaaac aagctccgct accttttcca gcagccgttg ttaccgtggt gcaatgcttg
       61 cggaaaagcc gaaaaagcgt tggcgttttt ggtatgcgtt tgtgaatggc ttgttttttg
      121 ataaaaagca ttgctatgca gcctataaag cggaggtgta tcgaaaacag tatccaccgg
      181 agtttaccga aatcacttaa tcctgttaaa ccccatttca cagcccgaac cgctcgcaac
      241 ccgagcggtt attttttaaa gtttacccca acattcaacc ctaacctttc ccaattaagg
      301 aatactatgt caattttaga tacctatcta cacggtgttg aggtggttga ggtcaatgca
      361 ggtggtgtca ccatttccac cgctgctact tcggttatcg gagtagtttg caccggagac
      421 caagccgatg ccgagacttt tccgctgaat acgcccgttt taattactaa cccgttaaat
      481 taccttgaaa aagcaggtag caccggcacg ttacgccgca cacttaattc gattggctca
      541 atcgttaaaa cgccaacggt gatcgtgcga gttgccgagt cggacgatag cgacacctta
      601 actgcaaata ttgtcggcac gcaggaaaac ggcaaattta ccggtattaa ggcgttatta
      661 accgctcaat caaccgtgtt tgttaagccg aaattgcttt gtgtaccaca gcacgataat
      721 caagccgttg cgaccgaact attaagcgtg gctaaaaagc tgaatgcgtt tgcgtttatt
      781 tcagacaacg gtgcaaccac caaagaacag gcatatacct atcgccagaa tttctctcag
      841 cgtgaaggaa tgatgatctt cggtgactgg aaatcttata acactgataa aaaagcctat
      901 gataccgatt atgcagtggc ccgagcttgt gcgttgcaag cctatatcga caaaactgtc
      961 ggctggcata aaaacatctc aaacgtagag ctagacggtg taaccggtat cactaaagcg
     1021 gtagagtttg atattaacga gagctcaacc gaggcaaact acctgaacga aaaaggcatt
     1081 accatttgct taaaccataa cggtttccgc tattggggtt cccgcactct tgccaccgat
     1141 acccgctggg cgtttcagca gtcggtgcgg acggctcaaa tcattaaaga gacaatcgga
     1201 gcaggcttag cttgggcggt ggatatgcca ctcacaccgt tgcgtgtgaa aacaatgcta
     1261 gaggcgatta acaacaagct acgctcttgg gcatcgggtg atgaccctcg cattttaggt
     1321 gctcgtgtgt gggtagctga agagatcacc gcagatatta tcaaatcagg caaatttgtt
     1381 attaaatacg attaccattg gattccgtcc ctcgaaagcc taggcttaga gcaacgggtc
     1441 aatgatgaat atgtagtgga tttagtcaat acacttaaag cgttatagag gtaaaaatgg
     1501 gattaccggc aaaacttaag aattttaatt tttcggtgga cggcacaagc tatttgggcg
     1561 aaaccaccga agtcacacag ccgaaactgg ctatgcaact tgaagactac cgagcaggcg
     1621 gaatgattgc ccctgtgggt gtcaatatgg gtttagagaa actggagctt gagttcaaaa
     1681 tcggcggaca cgaagaggat ttgctcaagc tatttggtgg ctcaatcagt ggtaatgcgt
     1741 tccgtttcaa cggtgcgtac cagcacgacg atgacgattc agtcgatgcg gtagagttag
     1801 tctgccgtgg gcgaatcgct gaaattgacg aaggctcaag caaagccggt gatgacaccg
     1861 agcatagcta taaagcctcg ctaacctatt acaaaaaaac ggtgaacggt gtcgatatta
     1921 tcgaaatcga caccctcaac caaatttaca ttgtcgatgg caaagaccgc ttagcggaaa
     1981 ttcgtaaggc aatgggttta tagattttcc cctaaatttt cccaaagccc cttccccttt
     2041 tttcaagggg cttttaatga taaataatta aggattaaac aatgaaaaaa gtaacattaa
     2101 aacaaggcat tttgcgtggt gaaaaacgta ttaccgagat tgaggtgcgt aagccattaa
     2161 ccaaacaact gcgtggcaca aatcttacct cattaatgca gttaagcgtt gatgagtggt
     2221 gtattgtact gccacgcatt accacgccaa aattagacaa agccgatttt gccacaatgt
     2281 cggcagcgga tttgctaaaa ctctccggca aagccttaga tttaatgagc gaagacttcg
     2341 atgaggcaga cgaagaggac agcgaagaac agggaaaggg cgagatttta gcctaattcc
     2401 acagtttgtt gatgacgcta ttgctgatat tgctaccgtg tttcattgga cacccaacac
     2461 ctttgatgaa atgacgattg tcgagctagg gcaatggcga gaaaaggccc gcttacgaaa
     2521 tggggtggaa agttaaaaca agcggtcgtt tttcgcaaaa aatttgcaaa actcgac
//
LOCUS       DANLUA010000241         2576 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLUA010000241 DANLUA010000000
VERSION     DANLUA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            95..1210
                     /gene="wecB"
                     /locus_tag="PXB44_002469"
     CDS             95..1210
                     /gene="wecB"
                     /locus_tag="PXB44_002469"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL5282897.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
     gene            1225..2496
                     /gene="wecC"
                     /locus_tag="PXB44_002470"
     CDS             1225..2496
                     /gene="wecC"
                     /locus_tag="PXB44_002470"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5282898.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKGNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMHELIENDRIVGGMTNKCSVQAVNLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDHKPEWVISKMNEAVIYALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYPNQVFAVEPNVEELPKKLANKNISLISVDEALEVADV
                     VVVLVDHSEFKLVKPVFLNDICVVDTKGIWR"
BASE COUNT          834 a          493 c          497 g          752 t
ORIGIN      
        1 acattataat gaacacgatc tttataacta atttaacaaa attacttaca tctagtgtat
       61 tttagtttta tctatatttt taaaggagta ataaatgcgc tatctcatcg tcttcggtac
      121 tcgtcctgag gcaatcaaaa tggctccctt agttgagggc tttaagaaaa gcggtctaaa
      181 ttttaaagtc tgtgttactg ctcagcaccg ccaaatgctg gaccaagttt tagaattatt
      241 cgaaattact ccagattatg acctggatat tatgagcaat aagcaaacac tttctactgt
      301 aacttctgct attttagaaa aaattcaacc tgttattgcc gaatacaaac caactactat
      361 ttttgttcat ggcgatacag caaccactct tgcctcttca ttagcagcct attacaacca
      421 aattgatatc gctcatattg aggcaggtct aagaacgcat aatatctact ctccttggcc
      481 ggaagaaggc aatcgcaaat taacagctgc tttggcaaaa taccatttcg caccaacaca
      541 aagcaccaag aaaaatctac tcaacgaaaa tattgctgag ggcaccattt atgtaacggg
      601 taatacggta attgatgcct tattcctcgc ttgcaaaaaa ttagatgaaa atattgattt
      661 attaagctcg ctacaagaga aattttctta tataaaaagt aagcgtacta ttttgatcac
      721 cggacatcgc cgcgaaaatt tcggtaatgg ttttgagaac atctgtaaag ccgtttcaac
      781 attagcgtct gatttccctg acgttcaatt tatctacccc gtacacctca acccaaatgt
      841 aagagaaccg gtcaatcgcc tattagcgga taaatccaat gtgcatctca ttgagccttg
      901 cgactatctc tcctttgttt atttaatgag agagtcttac cttatcttaa ctgactccgg
      961 aggtattcaa gaagaagccc catcattagg aaaaccggta ttagtaatga gagacacaac
     1021 agaaagaccg gaagctgtag aagccggaac agtaaaactt gttggaactg cacaatctga
     1081 aattattaaa caagttactg agttactcaa tgatcaagct gcttatacaa aaatgtctga
     1141 ggctcacaac ccttatggcg atggcacagc tgtgaaacaa attctctcag tatttcaatc
     1201 aaaacaataa taagggataa atgtatgtcg aactttaata ccatttcagt agtaggctta
     1261 ggctatattg gcttaccaac cgccactgta tttgcccaac acggtctaaa tgtaattggc
     1321 gtagatgtta atcagcacgc cgtagaccat atcaatcaag ggaaaattca cattgtcgag
     1381 cctgatttag atgttgctgt tcaccaatgt gtatctagcg gaaaattaaa agccacactg
     1441 acacctgaac cagcagaagc atttttaatt gctgtaccaa cgccttttaa aggcgaggat
     1501 tatgagcctg atttaagtta tattcaagct gcttgcaaag caattgcacc tgtgctggaa
     1561 aaaggtaatt tagtcatttt agaatcaacc tctccggttg gtgcaacaga acagatggct
     1621 gagtggcttg ccgagtaccg tccagagcta agcttccctc agcaagcagg tgaagattct
     1681 gatattcgta tcgcccattg cccagaacga gttctaccag gacaagtgat gcatgaattg
     1741 attgaaaacg accgtattgt gggtggtatg acaaataaat gctctgtgca agcggtcaat
     1801 ttatacaaaa cttttgtaaa aggcgactgt atagttacca atgcccgtac cgcagaaatg
     1861 tgtaaattaa ccgaaaatag tttccgtgat gtcaatatcg cttttgcaaa tgagctttct
     1921 attatttgtg atcgcctaga tattaatgta tgggaactaa ttgctttggc taacagacac
     1981 cctcgagtca atatcttaca accgggttgt ggtgtaggtg gacactgcat tgctgtagat
     2041 ccttggttta ttgtgaataa aactcctgac ttggcaaaaa ttatccgcac tgctcgtgag
     2101 gtaaacgatc ataaacctga atgggttatc tcaaagatga atgaagcggt aatttatgcc
     2161 ttacaaaaaa caggtaaatc tatcgatcaa atcaaaattg cttgtttagg gttggcattc
     2221 aagcctgata ttgacgattt gcgtgaaagt ccggcattaa aaattactga aaaattggcg
     2281 gaaaagtatc caaatcaagt atttgcagtt gagccgaatg ttgaagagct accgaaaaaa
     2341 ttggctaaca agaacatttc tttgatttct gttgatgaag ctctggaagt agctgatgtt
     2401 gtagttgttt tggtggatca tagcgaattt aaactagtca aacctgtgtt tttgaatgat
     2461 atttgtgtgg tggatactaa aggaatttgg agataagtga tgaaaaagaa acagcaatat
     2521 cgtaaatcca ctattaacaa tactaatgca tcacaacaaa attcaggcga agtatt
//
LOCUS       DANLUA010000242         2571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLUA010000242 DANLUA010000000
VERSION     DANLUA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..395)
                     /locus_tag="PXB44_002471"
     CDS             complement(<1..395)
                     /locus_tag="PXB44_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5282899.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQR"
     gene            complement(499..963)
                     /locus_tag="PXB44_002472"
     CDS             complement(499..963)
                     /locus_tag="PXB44_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL5282900.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1172..1435
                     /locus_tag="PXB44_002473"
     CDS             1172..1435
                     /locus_tag="PXB44_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL5282901.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1519..2544)
                     /locus_tag="PXB44_002474"
     CDS             complement(1519..2544)
                     /locus_tag="PXB44_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5282902.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          706 a          580 c          494 g          791 t
ORIGIN      
        1 tctcgttgct ttgattgttc ttcgggagct acattcgggg gcaaaatata gctttgacga
       61 actttagctg aaaaagcaat aacttctaaa ccgtgatcaa gcattccacc gatatgtgaa
      121 tgatggtgtg attcagaagc tggtaatagt tgaaccatct cagcgtaacg ttctattggc
      181 tgctgataaa gtacctcaaa catttgacgg ttcattgata cctgttgcca aagtagccca
      241 agatattttt gtcgtaattc tgtattaagt aattgagagg ctttttccgg gtataaccac
      301 ccttcattat cttgctcaat agaaaaaata gtatcggatt tgaccgcact ttgagattta
      361 tttttaccaa aaaatttttt gaacattgaa cgcattggaa gagaccgaaa atatgaatgc
      421 tgtattgaat gaaagccgtt tagccctttg cccttttggg taagaccttt ccccttggaa
      481 aggttactct tatccccctt aggggattac aggatcgtac agttccgccg gtaaattttc
      541 acactggtct aaattgaaca gattgtgctt agcaatgaaa aagtgttgtt ccatttccgg
      601 atttccttcc tcgtcataca caatatttcc ctcctcatcg agtttttctc tctcattagg
      661 acggtagttc atcacaacag tagctttttc ccctttacgg atatatccgc cgagttcatt
      721 ggcttttttc gccgtaatcc acttacactg tttgtagccg tgttcagcgg tagcaatcca
      781 aagcaataac gtattgatac cggaatacag acggttagaa accgcattgc gaggtaatgc
      841 catattgcat tccggatggc tccacggttt taaccacgga gctgtacctt gttccaatgc
      901 agcaatgatt ctatcagtaa tatgttggta taaatcaaat ttaggttgtt gagtgttcat
      961 catgtgtttc tccaaagttt gggtaaaaaa atggggaaac acggttaccc tatggggaac
     1021 ttgtttcccc gatgggtagt ttataaaatt tcaatggcca caatttttct agaatcatca
     1081 aagccaacag aactgcctat ggcgtagtaa ttacgaatag tctgttcatc ggcattgatt
     1141 agcagatacc tatcgaggaa gagagattgg gatgagaccg gtaacgagaa ataatcgtgg
     1201 aaccagtttt gaccctcgtc acaataaata catcaataca ttggcaaccg cattattggc
     1261 acgttggcat attgagcaat tctctgagcg taatatccca gtcaatgatg aaaatctcta
     1321 tatggctcac aatattggct tagatggttt ccatcgagcg ttattagggc aatctactcg
     1381 taaagacatt gagaatatgc gtaagaatgg gatgaaacct tggatgtcca tttagctctg
     1441 ttttcaaatg acctaatcca gtttgttccg gagagagtac cgaaattttg cactctttca
     1501 tccgtggtgt atcaaacttc aacgtgctaa atgagacatt aaatatacat caacataatc
     1561 taccgcactc atcgttaaga aaccgggatt cccatcagaa tgaggaatat cgttaaagcc
     1621 cgataaggag acggttaaac aaaatggtgt taaattactt gcttgatttc gtttcacagc
     1681 tctgctttct ccactcgctc gcccattcga aatacgaccg tctgcaaaca caaaggtaat
     1741 gctcgttacc tcagaacgaa cgcaagatag cgtccaatct ccggttgatg taccggatac
     1801 aatcgccccc tcaacatcag gtaaatcaat gccattggca attaaatttt ctcgcccgat
     1861 taatacttta aacggaaacg gatctgtcac agcaccttga ataggtacac ggcctaacaa
     1921 tgccgtcatc gcagtagaac ccattaaagt cgaattttcg ggaatagtgt aataaggtgt
     1981 acgctttatt tgctgtatct gagtattacc agtcatattt tctgtcccag cattattatt
     2041 taatgctgtc ccagtatttg aactttcccc ctctgcttta aaagggcttg gaaagccggt
     2101 ttttgttttc ccggaataat tgcctgtaac cggtttacct tgttcatcgg ttaccaccat
     2161 atcatcagga tcgacccagc gaatgccatt gttatctaca ttccctgttt gtcctgctga
     2221 ttcagtccca aagccagaag aagcccctcc tacagggatt gcctctgctt ctttctgctg
     2281 ctgattacca ctttgaaccc gacctttcag ttcttcgatt tggcgattaa atccatccca
     2341 agcagcagcc aaacggcttt ctacacgact ttctacttca cccgcaatac gagaatccac
     2401 ttcggcttct cttgcttgta agcgtaatac ctcttgctca aagcgatcac cacgctcatt
     2461 tgctttacga gcttcttcac gagtatcttt taatgctcct accaacgttt ttaatgtatc
     2521 gtgaggcgta tctcctgcag taatacctaa tgtattcgcc tcctcatcac t
//
LOCUS       DANLUA010000243         2560 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLUA010000243 DANLUA010000000
VERSION     DANLUA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            43..2391
                     /locus_tag="PXB44_002475"
     CDS             43..2391
                     /locus_tag="PXB44_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5282903.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
BASE COUNT          747 a          517 c          624 g          672 t
ORIGIN      
        1 tttttaagga gaaagattgt gtttaagaaa acaggattgg cattgctgat ttcggctcaa
       61 tttagcccgc ttgtttgggc ggaaaataac gatgtagcgg tgttagatga agtttcggtc
      121 gttggcagca ctccaagtat atcgcagggg agtgaagtta ccttgctaaa agtcagtgat
      181 aaaattattg caggtaagga atttaaaaag cgttcggcaa cgctgggtaa tgcgttggcg
      241 tcagaattag gcgtgcatag taacccgttt ggtggcggag cgagtaagcc gattattcgt
      301 gggcaggaag gggcgagaat tcgcattttg caaaacggtt cagacgtgat tgatatgtcg
      361 aacctatctc ctgatcacgc cgttgttgct gattcactgc ttgccgatca ggtcgaaatc
      421 ttgcgtggtt ccagcacctt gctttatgcc agcagttcgc cggtaggaat tgtaaatgtg
      481 gtggataaac gtattccgac cgctattcct gaaaagggat atgaggttga actcaatagc
      541 cgttttgata ccgccgccaa agagaaagtc ggcgcattag gggcaacctt tggcatcggt
      601 aaccacattg cggtgcgagt cgaagggctg agtcgtcata gtgataacta ccgtgtgccg
      661 gggatcaact taggcgagcg cttaaactat gtgccggaca cttacaataa atccaaagtc
      721 ggcacgttgg gcttatcctt tgtcggtgaa cgaggctata ttggtgcctc atacagcaaa
      781 cggcgggata attatggctt gccggggcat aaccataaat ttgatttctg taccggtcat
      841 atctacggta ataagcggga taaatacgct tatacctatc tctatccgca tttgattggt
      901 gaggaaaata ttgggagcaa tccgcacttc cactgtggaa ccaaccacgc cgaggacggc
      961 acacacagcc acgataaccc gttcggacac gcccacgatc acactcacaa aggcccgtgg
     1021 gtggatttag aatcaaaacg gattgatgtg aaagccgagc tacgccagcc gtttaagggc
     1081 gtagataaaa ttaaggcaag ctatgctgat gccgattatt accacgatga aaaagatgca
     1141 ggcgtgcttg ccacccgtta ccataaacag ctgaaaaaag atcaggatta cggcaagccg
     1201 gtgaatattt tcaaaaatcg gggcaaaaat acccgcttgg aggtctatca cgcaccgcta
     1261 ggtggcttaa ccggtgtatg gggtgttcag tatcaaacgc aaaaaagcag tatgaatgcg
     1321 ccgaaagatc gcgaagtgaa attcccgctg gttgaaaata ccaataaaca gttcagcctg
     1381 tttggggttg agcagtatat gtgggatagc gttgctgttg agcttgccgg acgaatggaa
     1441 aagcagaaga ttgagattga gtatgatcgc aatgaaatca aacggttaca agaacactac
     1501 cgtatcagtg gcggcaaaca ggttgagcca gatctttcac cctatgatga aacggcttat
     1561 gcctattccg gcacgcttaa ctggttcttc caccctgact atcagctttc gtttactgcc
     1621 tcgcacaatg aacgcttgcc aacgccaatg gagctgtatt atcacgggca gcatattgct
     1681 actaattcgt ttgaatacgg caataaagac ttgaaaaaag agcagtcaaa taatgtggag
     1741 ttgggcttgg gctatcaagc tgaacgtatt ggctataaag tgagtgttta ttacaatcac
     1801 tttaaaaact acatctacaa tgaaaatctt ttccgtgaaa atcagctatt tatgcgccgt
     1861 tataatcagg caaaagcccg tttttacggg gtagaagcag aggcaagcta ccgctttaac
     1921 gaccaatacc aagccacgat tttcggcgat atggtacgag gctggctaac aaatttacca
     1981 ccattgaaga taaatagcga ttatagtgtg ttcaaagatt atctgccgga aaatgtggaa
     2041 aaagggaaag attatcttct atatcgggct gaccaaaaca cccctcgtac accgccaatg
     2101 cgtttaggtt ttcgctttaa tgcggaattt accccaaatt ggtcggggga tttagagctg
     2161 atccgcacct ttacccaacg ccgtacgagc cagttggaat atattaccga aggtaatacg
     2221 atgttgaatg tcgggttgtc gtatagcaat aagtggaaag agctggatta caaaatcagt
     2281 ctaaacggca caaacttact gaatcagccg gtgtatatcc acacttcgta tcaccaattt
     2341 gtaccacaaa tgggacgaaa ttttatgttg ggaatggaga tgaaatttta agttctcgac
     2401 ataacaagac aagcggttat tttttcttca aattttgcaa aaaaacggct aaaaataacc
     2461 gcttgtttaa aactcaggta ttggtaattt ggataaaata gtgctgtttt tcctttcttg
     2521 ttattttcgg agcttaccaa aatttcctgt ttgttgttaa
//
LOCUS       DANLUA010000244         2534 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLUA010000244 DANLUA010000000
VERSION     DANLUA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1642
                     /locus_tag="PXB44_002476"
     CDS             <1..1642
                     /locus_tag="PXB44_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL5282904.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
     gene            1632..>2534
                     /locus_tag="PXB44_002477"
     CDS             1632..>2534
                     /locus_tag="PXB44_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5282905.1"
                     /translation="MAFNQYVFSDIVELISEKIKIKDLKKENYISTDNMLPNFGGITL
                     AENLPNSASCNRFAKKDILFSNIRTYFKKVWLAEFSGGCSPDVLVMRSKNTDILLNEY
                     LFLLIRSDDFINFTVISANGAKMPRGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKI
                     QLNTQTNQTLEAIAQAIFKSWFVDFDPVRAKAAALSEGKSEHEANLAAMSVICGKDTS
                     ELNDTEYKALWQIAEAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTPPRKESEWFSDN
                     ANDTEWISIKDMGNQGLFITESSEY"
BASE COUNT          799 a          591 c          523 g          621 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aatcagtatg tattttcaga tattgttgaa ttaatatccg
     1681 aaaaaataaa aatcaaagac ttaaaaaaag aaaactatat ttcgacagat aatatgctgc
     1741 ctaattttgg tggaataaca cttgctgaaa accttccaaa ttcagcttct tgcaataggt
     1801 ttgccaaaaa agacattttg ttttccaata taagaaccta ctttaaaaaa gtttggcttg
     1861 ctgaattttc aggtggttgt tctcctgatg ttctggtaat gcgcagtaaa aacacagata
     1921 ttttattaaa tgagtattta tttttactta tccgttctga tgattttatt aactttacag
     1981 ttatatcagc aaatggagca aaaatgccac gaggggataa aaatgcaatg aaaggtttta
     2041 ttttcaatat cccaagtatt gaatatcaaa aaaaatgtat tgctaattat tttgcctttg
     2101 accaaaaaat ccaactcaac acccaaacca accaaaccct agaagccatt gcacaggcaa
     2161 tcttcaaaag ctggtttgtg gattttgacc ctgtgcgcgc caaagccgcc gccctaagcg
     2221 aaggcaaaag cgaacacgaa gccaaccttg cggcaatgtc ggtgatttgt ggtaaagaca
     2281 ccagcgagtt aaacgacacc gaatacaaag cgctttggca aatcgccgaa gccttcccaa
     2341 gtgagtttgg agatgaaggg ttacctattg gctggaaatt caatcaagca gacaatttat
     2401 ttgatgttgg tattgggaaa accccaccaa gaaaagagag tgaatggttt tctgataatg
     2461 caaatgatac agaatggatt tctattaaag atatgggaaa tcagggatta tttatcacag
     2521 aaagtagcga atat
//
LOCUS       DANLUA010000245         2531 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLUA010000245 DANLUA010000000
VERSION     DANLUA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..345
                     /locus_tag="PXB44_002478"
     CDS             <1..345
                     /locus_tag="PXB44_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282906.1"
                     /translation="IIRKKTAVANAILALNEVDSRKSSGVQLCDLLLGAILSSYQNDI
                     TSERKKELTKWIALHLGWDKLTYDTLQEEKKFNIWYFYDPTKGPRIVRTRETKLKYPL
                     LKKKEQTFQPAR"
     gene            complement(528..1766)
                     /locus_tag="PXB44_002479"
     CDS             complement(528..1766)
                     /locus_tag="PXB44_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDL5282907.1"
                     /translation="MDFLTELTNHNHRTPAVSVTVRPKPSKDNEGEKAKDISTLITHR
                     LIQLTLTDNRGFEADQLDLELDDTDGLLALPSRGAILSVGLGWQNSPLTYKGEYTVDE
                     LTHDGPPDKVTIRARSADLRGTLTNRHERSFHRTTIGKIVKQIAEENKLKPMVGKEFE
                     NEEVKHIDQTNESSINLLQRLAEQFDAIATVKNGNLIFIKAGNATTASGKLLPLFRIT
                     RSSGDSHSFSIAEGDNYKAVKAYWHNTQTGKRGEVTWDENSQVKKVTKPTMRKKTKVK
                     RGADGKPIKGKDGKSIKETVFVKGKGRQVNAVVQSKPIESDSEAIKTLPHTYATAQSA
                     INACKRNFEKLQRGVATFSLTLAEGNAELIPELPVQVSGFKAEIDSNEWIISQVTHSL
                     NKGGGFTTALEMELKPKSED"
     gene            complement(1766..2203)
                     /locus_tag="PXB44_002480"
     CDS             complement(1766..2203)
                     /locus_tag="PXB44_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282908.1"
                     /translation="MLQQFAMMSLGYFVFMRLTIPYQDTSREMNWSHVQNDVVGAMPK
                     SQFTGKAGETMEISAELRPEITGGKMSILALEMMAEQGSAYPLISGSTFMVLGWFVID
                     RISEQETTFFADGTPRAISFSMSLKRVDDSLLANIIDEVAGFI"
BASE COUNT          695 a          562 c          524 g          750 t
ORIGIN      
        1 ataattcgta aaaagactgc cgtagcaaat gctattctag cattaaacga agtagattct
       61 cgtaaatcat ctggcgttca actatgcgat ttattattag gagcaatatt aagtagctat
      121 caaaatgata ttacatcgga acgtaaaaaa gaactaacaa aatggattgc tttgcaccta
      181 ggttgggata aattaactta tgatacttta caggaggaaa agaaatttaa tatttggtat
      241 ttttacgacc ctacaaaagg acctagaata gtaagaacga gagaaacaaa acttaaatac
      301 ccactattaa aaaagaaaga gcagaccttc cagcctgcac ggtgagtgtc ctcaaggact
      361 tatatgtgta aaacaacgcc caatttcctc actgggcgga atcacacaaa gtagccgccc
      421 ctatgattct ttgtgtttta tgtaaataaa tctgtaactt tgattaatat tacttgttca
      481 agatcagaga tgcaaggctt ttttatagtg attttataaa aatagcgtta gtcttcagac
      541 ttcggcttca actccatctc taacgctgtc gtaaacccac cacctttatt taaactgtgc
      601 gtaacctgcg aaattatcca ctcgttgcta tcaatctccg ccttaaagcc tgaaacctga
      661 accggtaatt cggggataag ctccgcattg ccctcggcaa gagttaggct aaaagttgcc
      721 acacctcgtt gtagtttttc aaaattccgt ttacaagcat taatcgctga ttgtgcggta
      781 gcgtaagtat ggggtagggt tttaattgcc tcgctatcac tttcaatcgg tttactttgc
      841 accactgcat taacttgcct gcctttgcct tttacaaaga cggtctcttt aatgctcttg
      901 ccgtctttgc cttttatcgg cttgccgtct gccccacgtt ttactttagt ctttttccgc
      961 attgtgggct tagtcacttt tttaacttgg ctgttttcgt cccacgtcac ctcgcctcgc
     1021 ttgccggttt gcgtgttgtg ccaataggct ttaaccgctt tgtagttatc cccttcggca
     1081 atcgaaaagc tatgggaatc ccccgaactg cgggtaatgc gaaacagggg taacagttta
     1141 ccgcttgcgg ttgtggcgtt tccggcttta atgaaaatca aattgccgtt tttcacggta
     1201 gcaatggcat caaattgttc tgcgaggcgt tgcaagagat taatagagct ttcgtttgtt
     1261 tggtcaatgt gtttcacctc ttcgttttca aactccttgc ccaccatcgg cttgagtttg
     1321 ttctcttcgg cgatctgttt cacaatttta ccaatggtgg tgcggtgaaa gctccgctcg
     1381 tggcgattgg tcagcgtgcc tcgcaaatca gcacttcttg cccgaatcgt gactttatca
     1441 ggtgggccat cgtgggttag ctcgtccacc gtgtattcgc ccttgtaggt cagggggctg
     1501 ttttgccagc ctaacccgac cgataaaatc gctcctcgac ttggcaaagc gagtaagcca
     1561 tcagtgtcgt ccagctctaa atccagctgg tctgcctcaa agcctcggtt gtcggttaag
     1621 gtgagttgaa ttaagcggtg cgtgatcagg gtggaaatat ctttggcttt ttcgccctca
     1681 ttatctttgc ttggctttgg gcgaaccgtc accgaaacag ccggtgtgcg gtggttatga
     1741 ttggttagct ctgttaaaaa atccattaaa taaaccctgc gacctcatca ataatattgg
     1801 cgagcagact atcatcaacc cgtttaagcg acatcgaaaa gctaattgcc cggggcgtgc
     1861 cgtctgcaaa aaaggtggtt tcctgctcgc tgattcgatc aatcacaaac cagcctagca
     1921 ccataaaggt tgaaccacta atcaacggat aagccgagcc ttgctctgcc atcatctcaa
     1981 gggcaaggat tgacattttg cccccggtaa tttcagggcg tagctcggct gaaatctcca
     2041 ttgtctcccc tgccttgccg gtaaattggc tttttggcat tgccccaacc acatcatttt
     2101 gaacgtgcga ccagttcatt tctcggctgg tgtcttgata agggattgtc aatcgcataa
     2161 acacaaaata gcctagcgac atcattgcga attgttggag catattattt aatcgttaca
     2221 gtttggctat gatatatgat gtaagaaatc aaaaataaaa ttgccgcaat aagatgctca
     2281 aagtaaacaa actgcaagca taacgcaaaa ttggatatga aactaaaaca agcataagct
     2341 tttgtttgtc ggtattcctg aattttaagt tcttcaatac caacacacat tattccaact
     2401 acaaggatga atcgcagaga aatgaagaac caccaaaaga acataaaggt agcttgagct
     2461 tgctcattaa gttgagtaaa aacaaataac gtaatgatat gaaaaatgaa tgctgtaatc
     2521 actttactga a
//
LOCUS       DANLUA010000246         2474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLUA010000246 DANLUA010000000
VERSION     DANLUA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..748
                     /locus_tag="PXB44_002481"
     CDS             <1..748
                     /locus_tag="PXB44_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5282909.1"
                     /translation="ERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLEEQGK
                     VSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGTTTLE
                     IAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEFLKNL
                     SIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFFVCPL
                     TVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(888..2279)
                     /gene="ffh"
                     /locus_tag="PXB44_002482"
     CDS             complement(888..2279)
                     /gene="ffh"
                     /locus_tag="PXB44_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL5282910.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          728 a          536 c          482 g          728 t
ORIGIN      
        1 agagagacaa cgagaaatac tacgacttat taataataat ggtgtcgcaa gtattaattt
       61 tctggcagaa cagctaaaag tatcacatat gacaattcgc cgtgatattc aaaagctgga
      121 agagcaaggt aaggtatccc tagtatctgg tggagttcaa tctcttgaac gattaatcat
      181 ggaattacca cacgatgata aatcaatgtt attccaagag cagaaatcag ctataggatc
      241 tttagcatca cagctaattc cctcaaacac aactatttat cttgatgctg gaactactac
      301 attagaaata gctcatcgga taatcgatag aaatgactta ctagttgtaa caaacgattt
      361 tgttattgct cactatctta gtaattcagg aaaatgtgat ttaattcata taggaggaag
      421 tgtctgtaaa gaaaacctat cgacaactgg ttcacttgca gcagaatttc tgaaaaacct
      481 atcaatcgat atggcattta tatctacttc atcctggaat ttaaaaggtc taacaacgcc
      541 gaacgaaaat aaaatccccg ttaaaaaagc aattttagag gcatccaagc aaaacatttt
      601 agtgacagac tcttcaaaat atggaaaagt cgcaactttt tttgtttgcc ctcttactgt
      661 atttgaccag attatttgtg ataaaggatt atttgagaat gtacgagacg cactgaaagg
      721 gctaagtatt aaattagatt taggataatc ttcttaccaa ttttttctga gaacgacaag
      781 caatcaaaaa gtattatttt tgcaaataag atattaatag ggaaagttaa caatcctagc
      841 aacggattgt taactgattt tcggctggta ctaataaaat aggaatatta acgtttacca
      901 aacatactgc ctaagccacc gagaccgcct agaccaccac ccatcatacc tttaacacca
      961 cgcatcattt ttgccattcc gcctttacgc atttttttca tcatacgttg catatcatca
     1021 aactgcttga gcagcttatt tacatcttgc acctgtgtgc cggagccaag tgcgatacgg
     1081 cggcggcgtg agcctttaat aatatccgga tttgcccgct cttttaaggt catagaattg
     1141 atgatggctt ccattttcat gaacatttta tcatcaacct gacttttaac gtggtcaggc
     1201 aggtttttcg cccccggtag tttatcgagc atcgacatca ttccacccat ttttttcatt
     1261 tcgataagct gctcacggaa atcttccaag gtaaattcat cgcctttttt gaatttggcg
     1321 gcaatttttt ccgctttttc ttgatcaacc gagcgttgaa tatcttcaat taaagacatt
     1381 acatcgccca tacctaaaat gcgggaagcc acacggtcag ggtggaacgg ctcaagggca
     1441 tcagtttttt cacccacacc taagaattta atcggtttac cggtgatttg gcgaatagaa
     1501 agtgccgcac cgcctcgggc atcgccatcg actttggtaa gaataacacc ggtaagcggc
     1561 aacgcttcgt taaaggcttt tgcggtgttg gcggcgtctt gaccggtcat cgcatcaacc
     1621 gtaaatagcg tttcaatcgg attcagcaca gcgtggattt gttggatctc ttccatcatt
     1681 tcgccatcaa cgtgtaaacg accggcagta tccacaatca atacatcaaa gaaattaagc
     1741 ttagcgtgct tcagtgccgc ttctgcaatc gctaccggtt tttggctggt ttcggtcggg
     1801 aagaagtcca cttttaacgc ttcagcaagg gtctgtagct gcttgatagc ggcagggcgg
     1861 taaacatcgg cagaaactac caacaccttt ttcttgtggc gttctttcag gaattttgcc
     1921 aatttgccga cagaggtggt tttacccgca ccttgcaaac ctgccattaa aattaccgca
     1981 ggcggttggg ccgcaagatt cagctcctcg ttggcttctc ccattgccgt ttctaattcg
     2041 ctttgtacga tttttaagaa ctcttgcccc ggcgtgaggc ttttgtttac ctcaacccct
     2101 aacgcacgct ctttgacctt gttgatgaaa tcacgcacaa cagggagggc aacatccgcc
     2161 tccaacaatg ccatacgaac ttcacgcagg gtatctttaa tattatcttc cgttaggcga
     2221 cctttgcccg taatgttacg cagcgtttta gacagtctgt cagagaggtt ttcaaacata
     2281 tcagctttct caatttgcaa aattttagga aaaaagtacc gcttgtgagc ggaaagttgc
     2341 cgccattata caaaaaaaag cgttacggtt atagggaaat aaaaaaacgg catagcgatt
     2401 aaactatgcc gtaaaatttg acgatattcg aggaatcatt atttagttat attgtgcgat
     2461 cataatactc cgaa
//
LOCUS       DANLUA010000247         2460 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLUA010000247 DANLUA010000000
VERSION     DANLUA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..168
                     /locus_tag="PXB44_002483"
     CDS             <1..168
                     /locus_tag="PXB44_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282911.1"
                     /translation="THATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTTLRQY
                     QPKGLGGLVRI"
     gene            404..895
                     /locus_tag="PXB44_002484"
     CDS             404..895
                     /locus_tag="PXB44_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5282912.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            922..1179
                     /locus_tag="PXB44_002485"
     CDS             922..1179
                     /locus_tag="PXB44_002485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282913.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(1176..2165)
                     /locus_tag="PXB44_002486"
     CDS             complement(1176..2165)
                     /locus_tag="PXB44_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5282914.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
BASE COUNT          770 a          537 c          445 g          708 t
ORIGIN      
        1 acgcacgcta ccgagtatca aaccacgctt ggcgtaatga agcggatact tgaaccgatg
       61 ttagccgact tcgaggtcga tccgtttgaa gaggctcact accacaaagc cctaactacc
      121 ttaaggcaat accagccgaa aggcttaggc ggcttagtcc gaatttaatt tgcaaaaaaa
      181 tcacacaatc cgaccgcttg tgaaacaaca agcggcggat ttccataccc aaattttgag
      241 gacaagccga tgaaatacgc aaaaatcatt ctatttttaa ccgcctttgc agttgctgct
      301 gaccatctag agttacgcaa cgactgcgat ggcaaaattt gtatggctca acgctaatta
      361 acccaaccgc ttgttccgca agcggttatt ttttggagga aaaatgcaaa caaaaccgat
      421 attagatgcg tgttgtggtt cccgtatgtt tcattttgac aagcaaaatc cactcgtcct
      481 gtttgctgat aatcgacaaa tagaaaccac aatgaaagac cgagacaaaa tccgccattt
      541 agaaatcaaa cctgatgttt tacacgattt cacgaatatg ccatacgaaa acgagagttt
      601 ttggcacatt gtttttgatc cgccacattt agttcaaggt ggagaaaata gctggcttgt
      661 aaagaaatac ggcaaattac cttgcaactg gcgagaagtc ttgaagaaag gctttgacga
      721 atgtatgcga gtgctaaaac caaacggcac gcttattttc aagtggaatg aaacacaaat
      781 ccccgtttca gaaattttaa aagttattga ttgtcagccg atttatggac acaaaagcgg
      841 acgcttaaac caaacacatt ggctggcgtt tgtgaaaatt gagcaatccc tctaaatgag
      901 gggtttattt ttggagagaa tatggaacaa attaaacttt cagaaaaagc ggagagagcc
      961 ttaattcttt ctcagctcaa cgaaaattca gcaatgatta ttgatttaga tgatgttgcc
     1021 ctgattttga attgcggata tgactatgtg caatccaaga tcagtaaaaa gcccggtttc
     1081 ccagagccag tgcaaggcat taacgggaaa tcagttcttt tcaaaagtgg cgatgttatc
     1141 cgttgggtca accgtcaagc acgaacacgc aaaaattaat ccagcaaatg agcgacttct
     1201 tccatatttg gggcgtagta agtgttaagc aaaatcttca catctttatg ccctgaaatt
     1261 ttggctaagg tcatcacatc gacttttttt gccaagcgtg aaagagcctc tctcctcgtg
     1321 tcgtgaaaat tcaaataaga aagcccagcc ttttctttta actcaaagaa tctagcactt
     1381 aaaccattcc cttgtggaag ctgaaaaacg gaattatcct ttttgatggt ttccagtctt
     1441 ttcaagatct cgattgcctt tgatgataac ggaacgtctc ttgatgaacc gttttttgtc
     1501 ataggcaaat gagctattct tttctcaaaa ttcacattat cccacgttaa acccgaaatc
     1561 tcaccggcac gcattgccgt ttcaatcgca aataacatag ccgcagccaa tcgagcatta
     1621 gagcgtttaa tatcttctcg gtatttgctg acttctaaga ttttatcaat atcttcctgg
     1681 gaataacgct gttttcttgg tggcactttg tttgggtgaa taacaccgat catcggattt
     1741 tcactaatat agccccagcg ttttaagcag taggtaaata cattgcttaa aatagacaaa
     1801 tccctctcaa cacttgagcc ttttaaccct ctcttcaatc tagcttcgat ccaatcttca
     1861 atatcatctc gcttaatatc ttgtacataa cgattcgcaa taggctcttt caatatgtta
     1921 ttgattcgac ctatctctcg tcgccacgtc ctcttattaa tcgatacttc attttgatac
     1981 cgctctaacg cttccgcaaa taaaatatca actaccccac ctttcgagat gatctctatt
     2041 ttacgctctt cttcgtaagc ccatgcctgt gcctctgctt ttgtgcggaa ggtagctgat
     2101 tttctcaccc ctttcttcga gatttcgact tgatatttac tgccccgctt gcgaatgctc
     2161 gccatatttt tacctgcctt ttgtcgtaaa tttgtcgtaa tttataccat acaataaccg
     2221 acaataacag ataataaccg attttatgaa atcatcttaa ggcaaagaag agaggaaaaa
     2281 cttaagataa cctattgaaa taactataat aaacgataat aaccgacaaa aaacggaaat
     2341 aaaaaaaagc ccctaatcaa aaaagcgaaa aggggcggaa gtcttaccta aggaaatatg
     2401 aaaaaaactt gcataaatct gttacatata ctcagactgc ctaactgaac gaaagttcat
//
LOCUS       DANLUA010000248         2393 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLUA010000248 DANLUA010000000
VERSION     DANLUA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..327
                     /locus_tag="PXB44_002487"
     CDS             <1..327
                     /locus_tag="PXB44_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5282915.1"
                     /translation="LVTINEKIVNLTYGDQDALNIYFKGGWGALPIEYNYQVDAILEL
                     VLRREQEELARKNGYLDVIPKIIHYTSKFKPWKKELHTMQISTRKKYWFYYHLEWNDI
                     LEQHQK"
     gene            complement(373..660)
                     /locus_tag="PXB44_002488"
     CDS             complement(373..660)
                     /locus_tag="PXB44_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5282916.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            780..1193
                     /locus_tag="PXB44_002489"
     CDS             780..1193
                     /locus_tag="PXB44_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL5282917.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            1278..2270
                     /gene="mglB"
                     /locus_tag="PXB44_002490"
     CDS             1278..2270
                     /gene="mglB"
                     /locus_tag="PXB44_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5282918.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
BASE COUNT          713 a          378 c          553 g          749 t
ORIGIN      
        1 ttggttacta tcaatgaaaa aattgtaaac ttaacttatg gcgatcaaga tgctctcaat
       61 atttacttta aaggcggttg gggagcattg ccgatagagt ataattatca agttgatgca
      121 attcttgaac tagtattaag aagagaacaa gaggaacttg cgagaaaaaa tggctattta
      181 gatgtgattc cgaaaattat ccactatacg agtaaattca aaccttggaa aaaagaactg
      241 catacaatgc aaatatctac gcgtaaaaaa tattggtttt actatcattt agagtggaac
      301 gatattttgg aacaacatca aaaatagcta tcatacaaaa gctgattcaa gtgtttgaat
      361 cagctttttg tattagaaac gtgttcctaa ttgaaaaata accacttctg cttggcagct
      421 aaatttgaag tgagcaacca gttcaaaacc gctttcaatc ggggtgattt cactgctgat
      481 ttcacagggt tcagattcga catcacgggc tttttgagtg aatttttcaa gtgccgcttt
      541 agcttcattt tcgctttcat aagtgcgctg aatagtagcg actgtttcgc tgttatctaa
      601 aatagaacct acatcaaatg tgttacagcc ttcgcattca atcggtttat cgtttttcat
      661 tggctaatcc ttctaaattg atgaataatg taggtacttt aaaactaaat tgcgtaacct
      721 gcaatcatca gtgaacgatt atttgattta gttggtattt tgcaaagttt ggaggaaaaa
      781 tgaccgcttg ggagcgtgtg tttcacgcgc tgttatttga atgtttcgct attattttta
      841 cggttatctt aacttcttgg ctgacttctc atcgtatggt tgatttaacc gcagtgattg
      901 tgatgatttc ggtgattgca atggtgtgga atgttatttt taactggggt tttgataagg
      961 tgtttaccgg tgagcgagtg caacgtggtt taggcattcg tattttgcat tcattccttt
     1021 ttgagggcgg tttgctggtt tttactatcc cattggtaat gtatatgctc gatatcggct
     1081 ggtggcaggc gtttgtgatg gatattggaa tgaccttatt tgtattagtt tattcggtag
     1141 tttttaattg ggtgtacgat catttacgcc tgaggtgggt taaatctggc taaattgtga
     1201 actagatcac ggtttcctta cacaaaattc gctatgcttg aattggttca tatttggcat
     1261 agcaagaggg acattctatg aagcgtacaa tcttgagtgc ttttgcactt tctgttattt
     1321 gcggtgtgaa tctaagttac gcacaatctg aaagcagagt gggggtaacg atttacaaat
     1381 atgatgacca ttttatgact caaatgcgta aggatatgca aaacgaggcg aaaggcttcg
     1441 ctgggttgaa gtggtttatg aatgatgcac aaaattccca agtgaagcaa ttagcgcaaa
     1501 ttgaaacctt acttcagcat aaaatgcgtg tgttggcggt gaatattgtg aatgcaaatg
     1561 agtcgaaaac tattgtggaa aaagcgaagg cttataatgt gccggttgtc ttttttaacc
     1621 gcacaccgag taaacaagcg ttagaaagtt acgaaaaggc gtactttgtg agcgctgatc
     1681 ctaaagaggc cggtagaatc caaggggaat taattgcgaa agtgtggaaa tcaaaccccg
     1741 atttcgatct aaataaagac gggaaattgc aatttgtatt gcttaagggc gagccgagtg
     1801 atgtgagcgc tgagcgttct caagctgtgg ttgaatcgct taatcaacaa ggtgttcagg
     1861 cagaagaaat ttacagtgac acggcgagat ggcgtagaac attggctcgc aataaaatga
     1921 atgagtggct ggtggaaaac cgtgcagctg agattgaagt tgtgatttca aacaatgatg
     1981 aaatggcaat tggtgcttta gatgcgttaa atgcacacga gaaaagattg ccgatctttg
     2041 gtattgatgc cttgcctgag acgttagcat taatgaaaaa aggggaaata gccggcacgg
     2101 tattaaatga tagctctgca caagcgaaag cggtggtgaa attggtatct aatttggcac
     2161 aaggtaagcc tgcgctgaaa gatacgcagt gggaaagcca ttcctctaaa acgatttatc
     2221 ttccacatat tggtattgat aaggaaaatt tagggcaatt tttaccttaa atggaggagc
     2281 ggggcgttat aaatacgccc cttattgatg ataacaagcg gtcatttttc agtaaaatct
     2341 tgcaaatcct ttctcttttc ttccaacaga tttgaataga gcgaatattc aaa
//
LOCUS       DANLUA010000249         2351 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLUA010000249 DANLUA010000000
VERSION     DANLUA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(220..2199)
                     /locus_tag="PXB44_002491"
     CDS             complement(220..2199)
                     /locus_tag="PXB44_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5282919.1"
                     /translation="MQSIFKKIFGKHTSQSAVKSDTVFSIEQDDDGWLYPQNATQLLN
                     TELRQKYLGLLWQQVSMNRQMFETFYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKARQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGEPPEPYKFKYIKGRDYHLHLVIGSFFANQIIPKSAFDWLAQYPEVISSL
                     MYAMSGHNDKAGLLSEIVQKADQYSVTLALGGDVNKLVQRPVNSLSKQLVLALRYLLE
                     QKIKLNTPKGPADGWFTEEGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLG
                     IVEESPSGGAIWSCRIQADSGWTPANVFTLLRIKPEVAWENINDRPNYFSGKVKIVEQ
                     IMEDSNMENHPPLVDESTKLVTLENVQPETIITSKEVEISQVEASSDNAPFSEIIIND
                     ENDLTSFVLDMFAPTESSEPSLADISIGKGQEIATMPSKQEVQDKPTTKKLNVTQSAV
                     ETPETLTLSELQSQNKENSITGDNFVNWLKSGIATNKFAINKPTAKLHIVEDHLFLVT
                     PVIFEMYLQESGLPYDKEMINSLQYDFQGLGIHLPKNTTINGKPDTINFWKCDVIGPR
                     KTSSLTGYLIKNTHLFFGDKTLLNNHCLTLQKEET"
BASE COUNT          669 a          468 c          448 g          766 t
ORIGIN      
        1 aattattcca tgttacagct ttgattttat cgccaacaat ttgattacgc cattcaatta
       61 gcatatcagt ggtaatctca tttgcattta actttgggta acgtcttttg ataattcgga
      121 ttacattatt atagctccgc ttagtatcag ggcgaagata gtaacgatag aaaaaaaaat
      181 cggtctagta gcgaatcaaa agagatagat gtcgtcattt taagtttcct ctttctgtaa
      241 agttaaacag tgattattta atagtgtctt atccccaaag aagagatgtg tattttttat
      301 caaatatcca gttaaagagg aagtctttct cggaccaatt acatcacatt tccaaaaatt
      361 gatggtatct ggcttaccgt ttattgtcgt attcttaggt aaatggattc ctagcccttg
      421 gaaatcatac tgaagactat ttatcatttc cttatcataa ggcagaccac tctcctgcaa
      481 atacatttca aaaataacgg gggtaactaa aaacaggtgg tcttcaacaa tatggagctt
      541 ggcagtaggc ttgttaattg caaatttgtt ggtcgcaata cctgatttga gccaatttac
      601 aaagttatct cctgtaattg agttctcctt attctgtgac tgtagctctg atagtgttaa
      661 tgtttcgggc gtttctactg cactttgtgt tacgttgagc tttttagtgg taggtttatc
      721 ctgtacttct tgtttggatg gcatcgttgc aatttcctgt cccttaccaa ttgaaatatc
      781 agcaaggctt ggctcagagc tttccgtagg tgcaaacata tccagcacaa aagaggttaa
      841 atcattttca tcatttatga tgatttctga gaacggtgca ttatctgagc ttgcctctac
      901 ctgagatatt tcaacttcct tagaggtaat aatagtctct ggttgaacat tctctaatgt
      961 aacaagttta gtgctttcat caactaatgg aggatgattt tccatattgc tatcttccat
     1021 tatctgctcg actattttaa ctttcccgga aaaataattt gggcgatcat taatattctc
     1081 ccaagccact tcaggtttaa ttctcaataa tgtaaagacg tttgccggtg tccaacctga
     1141 atcagcttga atacggcaac tccaaatcgc cccaccagaa ggagattctt ctacgatacc
     1201 gagagattgc atttcatcaa ataactttgg attatcctta ggcacggaaa tcccctgccc
     1261 tagtaagtaa gttcgtatat tatcagcggc agttttgcta acaagccaca atccttcctc
     1321 ggtaaaccaa ccgtctgcag ggcctttcgg agtatttaac ttgattttct gttccagtaa
     1381 ataacgtagt gccaagacaa gctgctttga cagagaattg acaggacgtt gaaccagttt
     1441 atttacatcg ccaccgagtg cgagggtaac actatactga tcagcttttt gcacgatttc
     1501 agataaaagc ccagctttat cattatgtcc ggacattgca tacatcagag aagaaatgac
     1561 ttctggatac tgtgccagcc aatcaaatgc agatttggga ataatctgat ttgcaaagaa
     1621 gctgcctatc acaagatgaa gatggtagtc tcgacctttg atgtatttga atttatacgg
     1681 ttcaggaggt tctccattcc aagcaaacca acgtgagcca tctttgagtg tgatttcaat
     1741 atctactaca actttgccaa tatcgtgaac taatgcaaga taaattgctg cggcagtcca
     1801 agcgtctcgc tgctttgact gttcttcggg agccacattc ggtggcaaaa tataactctg
     1861 gcgagcttta gctgaaaaag caataacttc caaaccgtga tcgagcattc caccaatatg
     1921 agaatgatga tgtgattctg aagctggcaa tagttgaacc atttcagcgt aacgttctat
     1981 tggttgctga taaaatgtct cgaacatttg acggttcatt gatacctgtt gccaaagtag
     2041 tccaagatac ttttgacgta attccgtatt cagtaattga gtagcatttt ggggatatag
     2101 ccaaccatcg tcatcttgct caatagagaa aacagtgtca gatttgaccg cactttgaga
     2161 tgtgtgttta ccaaaaattt tcttgaaaat tgattgcatt gaaagagacc gaaaagtatc
     2221 aattttgtgt taaatgaaag ccgtttagcc ctttgccctt ttgggcaaga cctttcccct
     2281 tggaaaggtt acctttcacc ctttaggggt tatcctttgc ccttaaggac tcctttatcc
     2341 ttttgccatt t
//
LOCUS       DANLUA010000250         2346 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLUA010000250 DANLUA010000000
VERSION     DANLUA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            32..751
                     /locus_tag="PXB44_002492"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXB44_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXB44_002493"
     CDS             811..1260
                     /locus_tag="PXB44_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL5282920.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            1361..1882
                     /locus_tag="PXB44_002494"
     CDS             1361..1882
                     /locus_tag="PXB44_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL5282921.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGK
                     EYHLYTPTHQQIVLKNKSLYPKSFINVDGVSGQLESKCIKLSLNKENEILQFEQLGRV
                     YVAAKNKRSTCKD"
     gene            1914..>2346
                     /locus_tag="PXB44_002495"
     CDS             1914..>2346
                     /locus_tag="PXB44_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282922.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQ"
BASE COUNT          800 a          392 c          466 g          688 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aagtgattta attttgcgat ctgcatcgaa aaatcaatat ttaatagttt acaaatgatg
     1321 cttaaattct aagataccct ctatcaatga gggtattttt atgtataaag ccatcacatt
     1381 aattgaaata ctaaccacta tcactattat ggtgatcgct gtttatttta tctcgccggt
     1441 tatttttaca ctgcaagatc gaattgcact tcatagtgaa attgaaaata tccaatcttt
     1501 tattcagcaa atccaaacta aagcacgtta taccaaacgt aattatacct taaccctttc
     1561 tcagcggaat aatcagtggt gtatgattgc ggtaattaag ccaatcaata ctagcaaaca
     1621 aattatttgt gattgtttga ataaacattt ttgtgattta ggaaaagaat atcatcttta
     1681 tacgccgact catcagcaga ttgtattaaa aaataaaagt ttatatccga aatcctttat
     1741 taatgttgat ggggtaagtg gtcaattaga atcaaaatgt attaagctca gtttaaataa
     1801 agaaaatgaa attttgcaat ttgaacaatt aggacgggtt tatgtggcag caaaaaataa
     1861 acgtagcacc tgcaaagatt agtttatctg cttttagcct aattgaagta ttaatggcta
     1921 gtgcattagg ggcgttttta ttattgcttg ttgcttcaag ttatgccgat ttctattaca
     1981 cacaaaccaa acaaaaagaa cagctctact tgcaagcaga agctcaccag ttactaaact
     2041 acttccaaca gcattttcaa cacattggct atcaaggctc tcacagaaca gacagtaatt
     2101 ttgatttatt tcagcaagca gggaaaagcc tgaatattgt gcataaaaat tgcattattg
     2161 gattttacga tttaaaccaa gatggctgtt tgggaaaacg taagacaaaa acggccgctt
     2221 gtaagttagg aacagtgaat aataccagag aggtgttaaa agaaattgtt ggctttaaag
     2281 tagaaaataa tgaaatttat actttttcat cgcaattaga taattgtgtg aaagaacaat
     2341 gccaaa
//
LOCUS       DANLUA010000251         2279 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLUA010000251 DANLUA010000000
VERSION     DANLUA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(23..2131)
                     /gene="gshAB"
                     /locus_tag="PXB44_002496"
     CDS             complement(23..2131)
                     /gene="gshAB"
                     /locus_tag="PXB44_002496"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL5282923.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKRKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPAKGQTVQLRANSNISTGGDSIDMTDQMH
                     ESYRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQ
                     GKSRRLTKAVIKMLFPEIGN"
BASE COUNT          621 a          499 c          473 g          686 t
ORIGIN      
        1 aaaagaggta tagagagaaa atttaattgc ctatctcagg aaacagcatt ttgattaccg
       61 ctttggttaa acggcgggat ttgccttggt aagggaaaat gtgcatcatc atcattgggt
      121 tgaagtttgc ctcaatgacg ccccaagagc ttaaatttgg ctcggcaggt ttggtgagat
      181 caggaatgat caaatccacc ccacacactt tagcccccat tgcctgagca attcccactg
      241 caatttgtct atagctttcg tgcatttggt cggtcatatc aatcgaatcg ccgccggtgc
      301 tgatgttgga attggcacgc agttgaactg tttgaccttt cgccggtaca cgcagtaaca
      361 ccgcaagggt ttcatcgccc agtacaaaga agcggtattc agtaccgact aaatagtctt
      421 ccaccatcac ctctttgtct tcacggaacg caatttcaac cgcttttgca aaatcttctc
      481 gatttttgac cgcttgttgg aagatggtaa tccctaaacc atagttagtc gatttcggct
      541 taatcaccac cgctttgcct tcaaatagcg gataatgtgc cacagcctgt tcaaggttgg
      601 taaattcaac gcttttcggc acgttaaagc cggcttttgc caatactttt ttcgtcacca
      661 ctttgttttc cataatcagc ggtgaaatgt attgatctcg gctggtcata ttgccgtttt
      721 tcacatattc caaatgatcg ccaaatttta gggcgaggaa ttgatcgttt tcgtccagta
      781 cttccacctc aatcccttgt tggatcaaat caaaaaacag ggcttgagtg gaaagttcca
      841 tattatcaaa tgccgataac gcataaaaac gctcaaaggc ttgtgcctta taggtttgag
      901 ccaatttagc ccccaatgct ttataactgc cgacttcctc tattgcggca attaaacgtc
      961 cgttcagggt tttgcttgga tcagcaaatt gagctaattt ttcccgcacc aaatctaggc
     1021 tttcctgacc ggcagataat tcttcaagca tcgctaaaag ttgatttaaa atggcttcac
     1081 cttcggcacg gaaagcggtt tctgttcttg gatcttcaag tgcgacttgg taaagtttcc
     1141 gtttgccgat ttcgacctct ttttgaccgc ttgtttcctc cagccaaagc ataccgagta
     1201 agaaataatg gataaatttg gcatcattca gctcaatgcc gtaaggggcg aaagggttta
     1261 aatcaaataa acggaattcg gcgtatttca caccgttttc caatagctca cgggcttttt
     1321 tgccccctcg taaacgcaca ttggaataga attctttttc ggcaattaat aatccgttgg
     1381 caacattggc ttcaaggctt tcaacatagc tttctaagct gtcgtgatta accaccactt
     1441 tttccgaatt cacataacca taagggcttg aacgcaagct gcggacaagt tgccctctcg
     1501 caagcggtga tttttcataa aaatattgcg attccaccgt aggcgtggca gccaataaat
     1561 agagcaaaat ccattgataa cgcaaaaagt tattagctaa cttcatataa agtgcgtttt
     1621 ggaaggcttt cagctcgcta tattccgttt gtaaagcaaa ggctttttcg acaaattccg
     1681 ttgaaagttg aaaattataa tgcacgccgc ttaccatttg cttatatttc ccatagtttt
     1741 tggaaaggtg ttggcgatat tgcacatctt cagggttgtc aaattgtgct tcttgaattt
     1801 gctcttccgg cggcaagcct gccggcatac tgagagggaa gatatattcc tcctctggca
     1861 acgaacgcaa taccacttcg tgaatagcag agagccaacg aaaactgtct tctaatttag
     1921 cattcggggg agtgattaac tctagctgac tttcggcaaa atcggtttga atataagggt
     1981 ggtaagagcg attaccgaat acggcagggt ggggagtagt aacgatattg ccgttgcgat
     2041 caacccgttg gctctctttc tcaagcccaa aattcccttg ttgaaacagc aatgctaaat
     2101 gattttctcg gataagtttt tgtagtttca tcatctatcc tttgtagtca aaaaaatgta
     2161 tcggcacaat aacacaaatg aaagacttag tgaaatagtg ggttttagtt tattggaatg
     2221 aagtagatga tttggttata acagaaatac tgcttcggat aaagcccctt ataccattg
//
LOCUS       DANLUA010000252         2269 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLUA010000252 DANLUA010000000
VERSION     DANLUA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..143
                     /locus_tag="PXB44_002497"
     CDS             <1..143
                     /locus_tag="PXB44_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL5282924.1"
                     /translation="PDLNALFGQLPESMWYFLNIFQIPMAISLMISAMISRFVRRMPF
                     IG"
     gene            153..692
                     /locus_tag="PXB44_002498"
     CDS             153..692
                     /locus_tag="PXB44_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282925.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKEGLRQLTECKN
                     TLVWGCVIVIGLMFFKERI"
     gene            623..1186
                     /locus_tag="PXB44_002499"
     CDS             623..1186
                     /locus_tag="PXB44_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282926.1"
                     /translation="MQKHVGLGLRNRYRVDVFQGKNLAKKNRTNSYQEKYDKKIFELY
                     KSYESDGAREAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESEVSHNT
                     EQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRLRLEPS
                     SQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            1183..>2269
                     /locus_tag="PXB44_002500"
     CDS             1183..>2269
                     /locus_tag="PXB44_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL5282927.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFSLLPKGWLSGKSNE"
BASE COUNT          765 a          312 c          469 g          723 t
ORIGIN      
        1 tacctgattt aaatgcgtta tttgggcaat tgcctgaaag tatgtggtat tttttaaata
       61 tttttcagat accaatggct atttctttaa tgatttcagc aatgatttca cgttttgtta
      121 gacgtatgcc atttattgga tagaggagtg ctatggctgt ttcagcgtat atagggattc
      181 ctggaagcgg caaatcgtat gaagtggtta agtcagtaat tttacctgct gttgcttcga
      241 atcgaagagt tgtttccaac atttatggat taaataaaga gaaaattcat caatatttac
      301 tgcgtaaaaa taaaaaatta atgattgaag agcttggtga agtcatatat gtaacgaatg
      361 aacaatgctt ggaatcggat tttttaccat caatggacga acaagattca ttttgcaaag
      421 ctggggactt gataattatt gatgaagttt ggcgtatttg gggaaacgac aaggatattc
      481 caaaaaatca tcgttcattt attgctgaac atcgacattt tgcagatcct ataacaggtg
      541 taacttgtga tttagtcgta ataaatcaag atatttctca aattccaaga tttattaaag
      601 aaggattgag acaacttacc gaatgcaaaa acacgttggt ttggggttgc gtaatcgtta
      661 tcgggttgat gtttttcaag gaaagaattt agctaaaaag aaccggacaa attcttatca
      721 agaaaaatat gataaaaaaa tatttgagct ttataaatct tatgaatctg atggtgcaag
      781 agaagcggtt acagatagta ggcaatctat attttcatct ttttgggtca aggtatggat
      841 tattctttta ccattggcat tattttattg tggctataac ctttacattt tcctaaatgg
      901 ttcagagagt gaagtaagcc ataatactga acaggctaaa tttgcttctg atgaaaacca
      961 taacgatata gtaattcatt tacttcctac tattggaaaa cctttatcaa ctaaatggcg
     1021 aataacaggg gaactaaaaa gggataataa atcatttgtg atcttagctg acaatgaaaa
     1081 ccgtttaaga cttgagccta gtagccagtt tagttttagc ggtcgaatgt tgcaaggaga
     1141 gatagatggt ggaatagtta gttatttttc gggagaatct aaatgaaaat ccatcaatta
     1201 gggctattgc tcatgattag ctcattttct tatgctaaga atgttgattt taaattagaa
     1261 gctgtgccgt taccgaaagc aatatcaata atatacgatg aagtgctaca aaagccatat
     1321 atgcttgatc ctaaattatc taatgattca agggttatta gctttcatac aacagagcaa
     1381 cagaattttg agcagtttat tcaacggtat ttcagcaata tgaatatcag tgtatcagag
     1441 aaaaaaggtg ttgtttatat tggacatatt gaaccaaaac agccgaaagt aataaaacgt
     1501 agttttgtgt attcgcctat tcatcgtgat gttgattatt tggctagctt cctgaaagat
     1561 ggaacagaag gaaaccaagt ttttgcaagt ggcgacaagc ttgtatatta tggtacttct
     1621 gaagatattg cacgagtgaa aagtatcttg aaatcaatcg acacaaaaag ccgtgaagta
     1681 gtggtaactg gttatgtttt tgaagtgcag aatattgaaa aagagggtag tggcataaac
     1741 ttgttagcga aattgctatc aggaaaatta ggcattagtg ttggttacaa gcaaagttat
     1801 gaaaacttta tcaccgtgaa tgcaggaaat ttagatgcaa tgtttgaatt gtttcgcaca
     1861 gatcagcgtt ttagggttgt cagcagtccg acattaagag taaaatcagg ctcaagaggt
     1921 aatttttcag taggctctga tgtccctgta ttatcgaatg taacctatca ggacggaagg
     1981 cctattcagt cgattgaata ccgttcaagc ggtgtaatat ttgatattca gccaacgata
     2041 aaagataaag cgattgattt aaaaattcaa caactatcaa atttcatcaa aacagatacc
     2101 ggtgtaaata atagcccaac gttggtaaag cgtgatctgg taacagatgt tacggttaaa
     2161 agcggtgatg ttatcgtttt aggtgggtta gctgaaaata aaatatctaa tggcgaaacc
     2221 agtttttcat tgctacctaa aggttggtta agtggaaaat caaatgaaa
//
LOCUS       DANLUA010000253         2213 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLUA010000253 DANLUA010000000
VERSION     DANLUA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            19..447
                     /locus_tag="PXB44_002501"
     CDS             19..447
                     /locus_tag="PXB44_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5282928.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            453..1175
                     /locus_tag="PXB44_002502"
     CDS             453..1175
                     /locus_tag="PXB44_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL5282929.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            1185..1604
                     /gene="psiE"
                     /locus_tag="PXB44_002503"
     CDS             1185..1604
                     /gene="psiE"
                     /locus_tag="PXB44_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL5282930.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            1613..>2213
                     /locus_tag="PXB44_002504"
     CDS             1613..>2213
                     /locus_tag="PXB44_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL5282931.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT          628 a          448 c          473 g          664 t
ORIGIN      
        1 aaatattaaa attttgttat gacacacgat tggctctatt gggcattagc ctctgcactt
       61 tttgcagcat taaccgccat ttttgccaaa gttggcttac aaggcgtaga ttctgatttc
      121 gccactttta ttcgcacggt agttattatt attgcactta ttgcctttct cacttacagc
      181 ggaaaatggc agccgttaga tagcttgagc agcaaaaact ggctgttttt aatcttatcc
      241 ggcttggcaa caggattgtc ttggtcggcg tatttcaaag cattgcaaat gggtaatgct
      301 tcccaagtcg ctccaatcga taaattcagc ttggtgttag ttaccctttt tgcagtaatt
      361 ttcttaggcg aacgcccaag cggacaagat tggcttggca ttggcttagt tgctggtggc
      421 gtactgttac ttgccttcaa gcggtgattt ttatgcaaaa ttttgcaaat attgaaattg
      481 cgtttattca tcggaaagca cctctgccga gtgatttctc ctaccctgaa atgcccggcg
      541 gtttaactga aaaacaggtg caaaaatggc aaagccgccg agtcgccttt tttctgctga
      601 cggaattgtt taaaaaacac catctggatt tagatttatt agctgatatg caacgctccc
      661 caagcggtcg cacttttgtc gattctgagc aaattgactt taacatcagc cattcagggg
      721 aatggatagc ggtcattttt tcgtattctt ttgcaaaatt agcggtcggc attgatattg
      781 aacacccaca aaaaatcaga cgttaccacg atttaatctg ccattacgga aacgctgaag
      841 aacaaaatgt tttattagcc aaccactccc ccattctaag caacctcgcc gaacgctttt
      901 atttgagctg gtgcttgcga gaggcgatat tgaaatcgca aggggtgggg attgtgaaac
      961 tgtccgaagt aaaacattta ccgttagaaa aacagatctt ttccgcccat tgtccgaaag
     1021 gcaagctgca ttttgtgagc gaactgccgt tctatttaag ctacttctat caacaacctc
     1081 aaaatatgct attatctgaa cctttgctat accattggca aaacgggcag tttcagccta
     1141 taaagtgcca atcacttgtt tatgatgtta attaaggata acgaatgcct aaaaaatctt
     1201 tagaagaaca ccattccagt tttggcaaaa ttgcttccga aacctttcag tggattatga
     1261 atattgcctt gctagtaaca ggagcattgc tttcttactc gctgtttgct gaagcctatt
     1321 cactctatga actttccttt agcgacagtg agaaattcca aattgtagaa aaaatagtaa
     1381 ttttctttct ctattttgag tttcttgcgt tgattgtgaa atatttcaaa tcgaattatc
     1441 actttccgtt acgctatttc ctctatatcg ggattactgc catggtgcgt ttgattattg
     1501 tcgatcattc taatgcgttg catacactat tattcgcact ctctattttg gtgatgatta
     1561 tagcattata tattgtacac accgatcgtt tacataaaag ctaggaacta atatgaagct
     1621 aacccaatat cagcacaatg atttacagat tctaaaccga gaaaccgtct ataaagggca
     1681 ttttgagcta caaaaaatca ctttccgcca taagctgttt tccggtgaaa tgagcggtga
     1741 agtggtgcgt gaactcttaa ttaaaggcga agcggcggct ttaattgcct atgatccaac
     1801 cttagataag gtagtattaa ttgagcaagt gcggattgga gcttacgatg caggacagaa
     1861 aaaatcccct tggttgctgg aattggttgc cggtatggta gataaaggca acgaagagcc
     1921 ggcagaagtg gcaatgcgtg aagccaaaga agaagccggc ttggaagtca caacagtgca
     1981 acacgcctta agcgtatggg acagtcccgg tggacaactg gaacgcttgc acctgtttct
     2041 cggcttgatt gacagcaccc aagtgaaatc cggcgaggta cacggcttac tggaagaaga
     2101 aaatgaagac attttggttc acgttgtaag ccgtgaacag gcatatcaat gggtagaaga
     2161 cggcacaatt gacaatgtga ttgcggtaat tgggctacaa tggttgcaat taa
//
LOCUS       DANLUA010000254         2200 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLUA010000254 DANLUA010000000
VERSION     DANLUA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1212
                     /locus_tag="PXB44_002505"
     CDS             <1..1212
                     /locus_tag="PXB44_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5282932.1"
                     /translation="TATKKPLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIYS
                     YISPKTGKRSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEKQ
                     ESIQSFAYKWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRLT
                     DLDRIMPDTLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEEL
                     AEFMQDLQKSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKGG
                     KNPHSVPLTKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKYK
                     GLLVAHGLRSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQE
                     WADFVEQCKSI"
     gene            1682..1888
                     /locus_tag="PXB44_002506"
     CDS             1682..1888
                     /locus_tag="PXB44_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL5282933.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            1898..>2200
                     /locus_tag="PXB44_002507"
     CDS             1898..>2200
                     /locus_tag="PXB44_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282934.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQEILFQQ"
BASE COUNT          827 a          457 c          407 g          509 t
ORIGIN      
        1 actgcaacta aaaaaccact ttcagacaca ttactaaaaa atctcaaata ttccccaaat
       61 gcgaagccta tcgcagacag caacgggcta tttattcttg cacagaaaaa agcgaaagta
      121 tggatttata gctatatcag ccccaaaaca ggcaagcgaa gcacaaaaaa tagaatcggt
      181 agctatccgc aaatgagctt agccgaagca cgcttaaaga gagacgaact aaataccctt
      241 gttttacaag gcttagaccc tttcgagcat ttagccaaaa tgcaaagaga agctaaggaa
      301 aagcaagaat ctattcaatc tttcgcctat aaatgggctg attggaagct atcaaaggga
      361 aaatgcaagc ctaaaacaat ggaaaaatgc ttacagcggt tagaaaatca tttattccca
      421 cgttttgaag gctatacgct ggaagatttt accttaaccg atacaatcaa ccgcttaacg
      481 gacttagatc ggataatgcc cgacactttg cacagaatag cagggaattt aatagaaatt
      541 cttgattatg cggtattgtg tgggagaatt aattttaacc caatcagcgt cattaaaaag
      601 gcgttttcca gcgtaagcac gacacaccag ccagcaataa tgcccgaaga gttagccgaa
      661 tttatgcaag acttgcagaa atcaaataga acgcctcaaa ttaaattatt agttgaatgg
      721 caactcttga atattcttag accgtttgaa gccgtagcgg tgcaatggag cgatatagat
      781 tgggaaacta agacgctcaa cattccggca gatagaatga aaggcggaaa aaatccgcat
      841 tctgtaccgc taacaaaaca agctatcgca atcctagagg aaatgaagaa atataacgga
      901 caccataaac atatttttac acaccgaaca gaccgaaaca aaccggctaa tagccaaacc
      961 gtgaataatg caataaaacg cttaagcggt ggaaaatata aagggctttt agtggctcac
     1021 ggtttaagaa gcattgccag cacctattta cacgaacgtt ttactactga aacgctagta
     1081 attgaggctt gtttatccca cacgaacaaa gacaccgtaa tgagtgcata ccaccgtaaa
     1141 aactggctag accgcagacg tggaataatg caggaatggg cggattttgt agaacaatgc
     1201 aagagcattt aaatgacaaa ggcggagcaa tccgcctttc tttttgcttg atgaaaaatt
     1261 ttcatttttt cctatcagct cccagcccgt gccgtctatc ccacgcctcg cccgcacact
     1321 taaagtctcg atttctacgc aaatgcaaaa ttaagcccaa acattgcccc gtatagcgtg
     1381 aaaaactttc atatagccgt tttttattac gcaattttac gcaagaaaac gcaaatttac
     1441 gcaggcagaa acaccacttt taaaaactcg atgacagaac gatgaaagaa ccgctgaaaa
     1501 cttgcaaaaa atcagagaaa aataaccgct tgcaagtttt ccaaatcaga attaagcccc
     1561 tctaatctgt aaaatctcct ttcacagccc taacaaatag caaaaaatcc aagatgattt
     1621 attctttcac acgttagcag gcaatagcac gccaaagcaa atttttacgg agtaaaacaa
     1681 aatggagaac acaccagcaa aacagcgatt tatgaaaata gatgaagttt cagccgtaac
     1741 tacgctttca gaaacgcaga tttatcgcaa aatgaaagcc ggcgattttc cccaaagtat
     1801 cagtattggg gcaaatagca aagtatggct agaaagtgaa atccagcaat ggattcaacg
     1861 caaaattgaa caacaccgca accaataagg gcaaattatg acacttgaaa catttaacca
     1921 acaaaaagct aaattccacg aacaagaaaa agccgtttta gctattcaga ccgaattaga
     1981 caaagcgaaa aatattcttg aggcattgca aaacgaaaaa gcggaatttg tcgcacggca
     2041 aaaaacgaat ttagccaata tcggcacgct ttcagcagat gaatatgttg aaatcaaaaa
     2101 taaaaatagt ggtttacaag cacgcataga atattatcaa gcgttgattg ttgatttaga
     2161 aaataaatgc tacgcagagc aagaaatatt attccaacag
//
LOCUS       DANLUA010000255         2188 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLUA010000255 DANLUA010000000
VERSION     DANLUA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            156..534
                     /gene="rnpB"
                     /locus_tag="PXB44_002508"
     ncRNA           156..534
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXB44_002508"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            595..1233
                     /locus_tag="PXB44_002509"
     CDS             595..1233
                     /locus_tag="PXB44_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL5282935.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            1224..>2188
                     /locus_tag="PXB44_002510"
     CDS             1224..>2188
                     /locus_tag="PXB44_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL5282936.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGT"
BASE COUNT          667 a          368 c          471 g          682 t
ORIGIN      
        1 gttcacttag acttcaaacg tgcgtaattt ttacgagtga attaaagcga agaggattaa
       61 gtttttaaat gaacttaatc cttttttatt ttgccataat aactacaagc ggttatttcc
      121 ttgcaatttt ttgcgaaagc attatactct agtgctgagt tggttaggca atcgctggtt
      181 tattgaagcc tctaaattcg tgaaaacgta aactggggga caagtaaacg agaggaaagt
      241 ccgagctaca cagggcagag tgccggataa cgtccggggg gcgtgagcct acgaccagtg
      301 caacagagag cagaccgccg gctttgtcgg taagggtgaa agggtgcggt aagagcgcac
      361 cgtgtgggtg gtaacattcc acagcagggt aaactccact cgtagcaaga ccaaatagga
      421 actcaatcgg tggcccgctg agagttcggg taggttgctt gagcgtatgt gtaaatgtac
      481 gtctagagga atgattgtcc acgacagaac tcggcttatc gaccaactca ctaaattctt
      541 gaaatgcgaa cagggggaac ttgttcgctt ttttatttta tagtgaaagt aaatatgaaa
      601 tttattaaga cgattatcac gttaattgca ttatattcaa tgccagtttt ttctcaccca
      661 cattcatttg ttgatttgaa aacgaatgtt attgtggaag gctcaatgtt gaaatctttc
      721 caaatggaat ggatgctaga tgaaattgct tcttctgagt taatttatga agtgaaaaat
      781 agccaagata aagaaaaaac tcagcagaat atcactgcag aaatggtgca aaccgcattg
      841 caaaatcatt attttagcac cttatatgat agtcaaaatc agccgataaa atttacgaca
      901 aagcccacag cgactcgctt tgaaatcaag aataacagag tgatttttta tattactttt
      961 catttagcta aacctcacga attaaagcag agtaaagtga gattctatac ttatgaaccg
     1021 agttattata ttgcgatgga atataatcgc cctgctgatg tcaataccag taatgcaagt
     1081 tgtaaagcaa cattggtgca acctcaagta gatagtaagt tacgcttata tgcttccggt
     1141 ttggataaaa accaatcact ggatatgcct gaaaatggag attactctct tggtgcccaa
     1201 tttgctcaaa aagtggagat tatatgcgat taaaagcaac atattgggga atgatagtga
     1261 taggattttt gagcctattt gctctttatc agctttatcc aatgatattg tttaaagtga
     1321 tggaatggca aaaaaacttt aatttacagc tttccgcttc gctcaatgaa ttagctgaaa
     1381 atcaaagtaa agccggtatt accttagtat ttattagttt tttatatgga gtatttcacg
     1441 cagttgggcc tggtcacgga aaatttattc tgagcagtta tctttctttt gaaaaaacaa
     1501 aattaaatca ggcgatgaaa atcacttttg cctcagcatt ggtgcagggc ttagtggcaa
     1561 tttcattggt gacggtgatt gtggtgattt ttactctctc tcgccaatat tttaatttaa
     1621 ccctaaaatg ggttgagcgg gggagctttg gtttgatgat cttgttcggc atttactggt
     1681 gttaccaggt gtatcgaacg gtgaacagag cgcgaaagcc tatcattaaa tccattcgta
     1741 tttcgcaaaa aaaagctgga aaattaccgc ttgtcacgca gaaagggcat attcataccg
     1801 aaaattgtag ctgtaggcat aaacatttac cgacttcaaa tgaaatgcaa caagtgagag
     1861 attggaaagc acagctgatg ttggtattaa gtattggctc aagaccttgc tctggcgcta
     1921 ttcttgtttt atttctttct tatacgctca atctctatgc ttggggggtt atttcagcat
     1981 tagctatggc agtaggaaca gggctgactt taagcttatt tgcttatctt gttatcattg
     2041 cgagaaataa ggcagttaag gttagccaat ggtatttttc agctcaaatt aatcgaaata
     2101 tcgtttggat attaaaattt acacttggac tcgcattaat tctatttgga atggtattat
     2161 ttcacagtag tttcattgat gctggtac
//
LOCUS       DANLUA010000256         2080 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLUA010000256 DANLUA010000000
VERSION     DANLUA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..431)
                     /locus_tag="PXB44_002511"
     CDS             complement(<1..431)
                     /locus_tag="PXB44_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5282937.1"
                     /translation="MRIIKKRYPKLNANDITTDMLIEWRNQIVGDKIKAVTWNNYVRH
                     LKALYNFGISQGLLNINRNPFNQLFIRESKRRKKTYSTEQLHKIDHILNNNVVLPDFL
                     KPSWFVLAIIMTLRCTGIRRGQLVKLKISDVDLSNRTIYISP"
     gene            complement(530..>2080)
                     /locus_tag="PXB44_002512"
     CDS             complement(530..>2080)
                     /locus_tag="PXB44_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL5282938.1"
                     /translation="VHDIGKVVVDIEITLKDGSRWFAWNGLPPQPYKFKYIKGRDYHL
                     HPVLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQKADQHSVT
                     LALGGDVNKLVQRPVNSLSKQLVLALRYLLEQKIKLNTPKGPADGWFTEEGLWLVSKT
                     AADNIRAYLLGQGISVPKDNPKLFDEMQSLGIVEESPAGGAIWSCRIQADSGWTPANT
                     FTLLRIKPEVAWENINDRPNYFAGKVKIVEQITEESNIETQSTLVDGTTKPAPLPSVQ
                     TGGIIASKEVEISQVETNSDNAPSSEIIINDENDLTSFVLDMFAPTESSEPSLDDISI
                     GKGQEIASMPSKQEVQDKSTTKKLKVTQSAVETPETLTLSELQLQNKENSITGDNFVK
                     WLKSGIATNKFAINKPTAKLHIVEDHLFLVTPVIFEMYLQETGQPYDKETINNLQYDF
                     QGLGIHSPKNTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTRLFFGDKVLLNNHC
                     LTLQKEET"
BASE COUNT          587 a          407 c          389 g          697 t
ORIGIN      
        1 ggcgaaatat aaatcgtcct attagaaaga tctacatcac taattttcag tttcactaat
       61 tgtcctcgcc gaatccccgt acatctcaat gtcataataa ttgcaaggac aaaccagctt
      121 ggttttagaa agtcaggtaa tacaacgtta ttattgagta tgtggtctat tttatgtaat
      181 tgttcggttg aataagtttt ctttctcctt ttcgattctc gaataaataa ctgattaaat
      241 ggatttcgat ttatattaag taatccttgc gatataccaa agttatataa ggcttttaaa
      301 tgtctgacat aattattcca cgttactgct ttgattttat cgccaacaat ttgattacgc
      361 cattcaatga gcatatcggt ggtaatgtca ttggcattta actttggata acgctttttg
      421 ataattcgga ttacgttgtt gtagctccgt ttggtatccg gacgaagata gtgacgatag
      481 aaaaaaaaat cggtctagta gcgaatcaaa agagatagaa gttatcattt taagtttcct
      541 ctttctgtaa agttaaacag tgattattta atagtacctt atccccaaaa aagagacggg
      601 tattttttat caaatatcct gttaaagagg aagtctttct cggtccaatg acatcacatt
      661 tccaaaaatt gatggtatcc ggcttaccgt tggtcattgt gtttttaggt gaatgaatcc
      721 ctaatccttg aaaatcatac tggagattat taattgtttc cttatcataa ggctgtccag
      781 tctcttgcaa atacatttca aaaataacgg gggtaactaa aaacaggtgg tcttcaacaa
      841 tatggagctt ggcagtaggc ttgttaattg caaatttgtt ggtcgcaata cctgatttga
      901 gccactttac aaagttatct cctgtaattg agttctcctt attctgtaac tgtagctctg
      961 atagtgttaa tgtttcgggc gtttctactg cactttgtgt tactttgagc tttttagtgg
     1021 tagatttatc ctgtacttct tgtttggatg gcatacttgc aatttcctgt cccttaccaa
     1081 ttgaaatatc atcaaggctt ggttcggaac tttccgtagg tgcaaacata tccagcacaa
     1141 aggaggttaa atcattttca tcatttatga tgatttctga ggatggtgca ttatccgagt
     1201 ttgtctctac ctgagatatt tcaacttctt tagaggcaat aatgccccct gtctgaacac
     1261 tcggtaatgg agctggttta gtggtcccat cgactaatgt agactgagtc tctatattac
     1321 tttcttctgt tatctgctcg actattttaa cttttccggc aaaataattt ggtcgatcat
     1381 taatattctc ccaagccact tctggtttaa ttctcaataa tgtaaaggta tttgctggcg
     1441 tccaacctga atcagcttga atacggcaac tccaaattgc tccaccagcc ggtgattctt
     1501 ctacaatacc gagagactgc atttcatcaa ataactttgg attatcctta ggcactgaaa
     1561 ttccctgccc cagtaagtaa gctcggatat tatcagcagc agttttgcta acaagccaca
     1621 atccttcctc agtgaaccaa ccgtctgccg gacctttggg tgtatttaac tttattttct
     1681 gttccagcaa ataacgtagt gccaagacca gttgctttga cagagaattg acaggacgtt
     1741 gaaccagttt atttacatcg ccaccgagtg cgagggtaac actatgctga tcggctttct
     1801 gcacgatctc agataaaagc ccggctttat cgttatgccc agacattgca tacatcagtg
     1861 aagaaatgac ttctggatac tgtcccagcc aatcaaacgc agattttgga ataagttgat
     1921 ttgcaaggaa gcctcctagc accggatgaa gatggtagtc tcgacctttg atgtatttga
     1981 atttataagg ttgaggtggt aacccattcc aagcaaacca acgtgaaccg tctttgagtg
     2041 taatttcaat atcaacgaca actttgccaa tatcgtgaac
//
LOCUS       DANLUA010000257         2054 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLUA010000257 DANLUA010000000
VERSION     DANLUA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(85..2046)
                     /gene="recD"
                     /locus_tag="PXB44_002513"
     CDS             complement(85..2046)
                     /gene="recD"
                     /locus_tag="PXB44_002513"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL5282939.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
BASE COUNT          578 a          446 c          359 g          671 t
ORIGIN      
        1 aaacaaaaac aacgcaagcg gtttttgtaa aaaatttgaa aaaaatgacc gcttgcgtat
       61 ttttacggca aaacgagtat taatttagct aaacttgcct cttatttgtt cttttagccc
      121 actttgtctt tcggttttgg caccaacact gtatttccaa actttttcgc taccaaataa
      181 ggtaaattta tcttttgccc ttgtgattgc ggtgtaaatc aactctttcg tcagcaccgg
      241 ggaaatcgaa tggggcaaca ccaataccgt atgttcaaat tcagaacctt gcgatttatg
      301 cacagtcata atataagctg cttcataatt tgaaacccta cctagaggca aactatggtg
      361 ttctctattt tcctgtaagg aatcaaaata aactcggctt tctccatttt cgtccggcaa
      421 aatgatgccg acatcaccgc tggctatgct atttatcggc atattttctg tcattaaaat
      481 cggttttccg atatagcggt ctcggctatg attaaatttc actaagctag ctttccgcaa
      541 gccttccgca atcgccatat taagacgttc aacccccagt tcacttaccc ttaaggctga
      601 taaaattcgg actttttgaa atttctcaaa aatcgctttc acactcactg aatctggatt
      661 tttctcccgt ttttgaaccg cttgtaaata atcacgataa agttcaaccg ctttatccac
      721 caccatttgc acactttttt caatccagct tgatttatcc gttgtctctt tcgtttccgg
      781 ataacagaaa ttttctaact tatcactgtt tttatatttg tcgaaaatct cccacgaaag
      841 gttaattttt tgatcattta ctgcatttgc aagctctcca atccaggctt tttcgccaaa
      901 tcgatagcta tgttttaagt gagataaaca atcaccaatc ggcaacgcat taccggcttc
      961 aatttggtag tcggtaacgg cttttaagta atcgcaatgg gattggctat aaccattatc
     1021 aataaaagag ccaagttccc ccataattga accagcttcc acagaagcaa gctgatcttt
     1081 atctcccagc attatcaagc gagagtttgg tttgagcgct tgaacaactt tctccatcat
     1141 tgataaatca atcattgaag cctcatctaa tacgaccaaa tcatagtgcc acggattttt
     1201 ggtatgatat ttggttttct cagttaatgg tctcgcacca agcaagcgat gaatagtcac
     1261 tgcttcagtt ggaatagagc gttttaactc ctcagataac cccttttctt ccgccaactt
     1321 gctttccagc gactctttca ttcttgctgc tgcttttccg gttggtgcag ccagagcgat
     1381 ttgcaaatat ggtttctgta gtgcttgctg ttttaattgc agcccgagta ataattttac
     1441 taccgtagtt gtttttccgg ttcccggccc acctgaaatt aagctaaact gcttttctaa
     1501 tgctgttgca acggcaatct tttgccagtc gatagtttga gctttcccag tgaatagttg
     1561 gttgagaatg ctttggttta attcggtatt tgcaaatttt tccgaaaaat caaccgcttg
     1621 ttgtaaataa ccggcaatgc gatgctccgc ttgccagtag cgataaaaat agagcaagct
     1681 gttttggaat aacatcggtt taattgtttc gggagaatag ctaaaagcga tatgattttt
     1741 cagcacactc tgccattcaa gcggtggaat ttgctcaatt ttttgcaaaa tttgttcgag
     1801 caaatgataa gtttgtggct tgctatttaa ttcaaaaaga gaagaaagac tgagattttc
     1861 caaacgtaat gccgtatgcc caagtcccac ttggtaagaa accaacgcag cggctaacac
     1921 agctaaatct tgctccaaag gcgaataccc atagggtttc tgcttattat caataaaacg
     1981 ggcaaattga taatttaagt cagaaatcgc cttttcttgt ttaagttcca ataataaatt
     2041 cagcataatc aaac
//
LOCUS       DANLUA010000258         2052 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLUA010000258 DANLUA010000000
VERSION     DANLUA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1693)
                     /gene="recJ"
                     /locus_tag="PXB44_002514"
     CDS             complement(<1..1693)
                     /gene="recJ"
                     /locus_tag="PXB44_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL5282940.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQ"
     gene            complement(1778..>2052)
                     /locus_tag="PXB44_002515"
     CDS             complement(1778..>2052)
                     /locus_tag="PXB44_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HDL5282941.1"
                     /translation="RYLAFPRGGMNTQTAKQMEAIWTSADKVKALNDAEDGKLPTQIK
                     TPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEPKELAKMLSE"
BASE COUNT          598 a          465 c          344 g          645 t
ORIGIN      
        1 attgcaaaga tttctctcct cgaaattcat taatatccaa cctataaact aatcttgcct
       61 ttttgatcga taagtcagga aacgccctta catccacatt aaaccaaatt gcatcaaata
      121 gttgcccttt ctctgattgc aacattaatt ttaaatgttt acctgcgagt aatttttgtt
      181 ggagaatgct aaactcccct tcaaaactag gttctggaaa atgttgtccc caaggcccac
      241 cttgcctaat catctcggca ttggctaaag taaactctag tgcatttaac tcaccatcgg
      301 tataaatgat gccttgtaat tgctcaggct cgataattgc attaatagat tggtcaaata
      361 tctgtttaaa taaatctaat tttgattgat ggatagtcaa accagccgcc attgcgtgtc
      421 caccaaattt ttcaatcaag tctggatgtc gcatatcgat atgttccagt aaatctcgca
      481 tatgtactcc gttgatcgag cgagcagaac cttttagata ttcactttcc tcactttctt
      541 gtgcaaaagc aatgacagga cggttaaatt gatctttaat gcgagaagct aagatcccaa
      601 ttacaccttg gtgccaatct gcttgatata acactattcc gtgtgcctgt tcagactgcg
      661 caagacttgg caaattcgca caaatcgaca gtgcttctgc tttcatttct tgttcaaact
      721 cttttcgggt ttgattaaga gaatcaagat caagcgcaag ccggcgagct aattccatat
      781 tttcacaaat tagtaactca acccctaaag acatattttc taaccgacca gctgcattca
      841 aacgtggtgc gatagcaaaa cccaaatctg ctgcttgcaa agtagaaaga tctcgcttgc
      901 ccacctccgc taaagctttt atccctaaac ggcaatgccc tgaacggatt cgctgtaacc
      961 cttgatgcac caatatccga ttattttgat ctaacggcac tacatcagcc actgtgccta
     1021 aagcgactaa atccagtaat tcagcaaaat ttggctctgg tttattttca aataaatgtt
     1081 gttctctcat acgagaacgc aatgccatca ttacatagaa gatcacgcct accccggcta
     1141 aagatttaga cggaaattca caaaaagcta aattggggtt taccagtgca tctgcattgg
     1201 gtaggctctc tggcggtaaa tgatgatcgg taaccaaaac ttgaatctga tggcttttta
     1261 gcagagcaat gccttcagtc gaagaaattc cattatctac cgttaagact aaatccgccc
     1321 cttttgctaa taccatttca gcaaccgcta cactcaatcc gtacccctga gaaaaacgat
     1381 ccggaattaa ataatctacc ttatcgaaac ccaattggcg taacgcaata agggcaagcg
     1441 atgtactggt tgcaccatct gcatcaaaat cacccacaat aatgatccgg cttttttcac
     1501 gaaaggcttg taatagcaag tcaacggctt gctcaatatt ggcaagttgc tgtggtctat
     1561 gcaaattctt taaattatgc tctagttctt gcgtattctt tatgcctcga ctttgataga
     1621 ttcgatttaa taacgggttt gtagagacta tttccgttgt taataatggg cgccttttaa
     1681 ttattttttc cacaatactc ttctttaatg aaacaacaag cggtcttctt ttgcaaaaat
     1741 tttacaaaaa aagaccgctt gtattcaagt taatgaatta ctcacttaac attttcgcta
     1801 attctttagg ctctacataa ccaccaatca actcaccttc tgaagttacc atatttgggg
     1861 tacctgtcac accaaattta atccctaatt ggtaatgttt tttcacaata tccggcgttt
     1921 ttatttgggt tggtaattta ccatcttcag catcattcag tgcttttact ttgtctgctg
     1981 atgtccaaat agcttccatt tgttttgctg tttgggtatt cattccacca cgcgggaatg
     2041 ccaaatagcg aa
//
LOCUS       DANLUA010000259         2034 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLUA010000259 DANLUA010000000
VERSION     DANLUA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            9..950
                     /locus_tag="PXB44_002516"
     CDS             9..950
                     /locus_tag="PXB44_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL5282942.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1046..1933
                     /gene="dapA"
                     /locus_tag="PXB44_002517"
     CDS             1046..1933
                     /gene="dapA"
                     /locus_tag="PXB44_002517"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL5282943.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
BASE COUNT          628 a          318 c          446 g          642 t
ORIGIN      
        1 aataattaat gagtgagaaa aaacagttac ctccattcca gacacaattt ttacacccta
       61 aatattgggg attgtggctc ggtttaggtg tttttaagtt aattttatgt ttgccttatc
      121 ctattttagt aaaaattggt ctgggactag gtaaattgtt tactaaattg ggttttggta
      181 aacgtcgtat gcgtattgct cgccgtaatt tagagctctg ttttccccat tactctgctg
      241 agaaagtaga ggaattagtc gaagaaaata tgaaatcggt tggaatggcg attattgaaa
      301 ctggtatggc ttggttttgg tcggataaac gtattcttaa atggtctaaa atcgaagggc
      361 tagagcattt aacacagcgt gatcaggaaa ggggtatttt acttgtaggg gtgcattttc
      421 tgactttaga actcggtgca agaattattg ggctacatca tcaaggagta ggggtttatc
      481 gaccgaatga taatccatta ttagattgga tacagtttcg aggacgtgtt cgctcaaata
      541 aagcgatgtt agatcgtaaa gatgtgcgtg gaatgattcg agctttaaaa aatggcgaga
      601 tgatatggta cgcgcctgat cacgattatg gaagacgaaa tagcgttttt gtaccatttt
      661 ttgcagtgga ggaaacttgt actactgcgg gtagttatat gttacttcgt tctgccccaa
      721 agagtttagt gatcccgttt agcccgattc gaaatttgga ctattcaggc tacacagtaa
      781 aagtcagtcc tgccgttgat tttagtcaat gtcaaaatga agttgaaacg gctattttaa
      841 tgaatcgggt ggtagaacgt gaaattatgg ctgcgctaac tcaatatatg tggctacatc
      901 gccgttttaa aactcgccca aatgaaggtg atcaaagcct atatgattaa ttttaaataa
      961 tttcaaaaaa gtgcttgcat atttaagctg aattttgcaa tctagcaatg ttatcaagca
     1021 taataaaaaa atacaggaga tgattatggc gaccccttta tttcacggta gtatcgtagc
     1081 attagttaca ccaatgactc atggtgaggt agattatcag gatttaaaaa aattagttga
     1141 gtatcatatt caagcaggtt ctcacgctat tgtatctgtt ggtacaacag gtgaatctac
     1201 cacattaagc attgatgaaa atgttaaggt gattaagaaa acggttgaat ttgctgatgg
     1261 acgtattcca attattgcag gaactggttc taatgcgacc agtgaagcta tcactatgac
     1321 taaattatta gcaaacagtg gtgtagctgg ttgtttaagt gtagtgcctt attacaacaa
     1381 gccaacgcaa gaggggatgt acttacacta caaagcgatt gcagaaagta cagatttacc
     1441 acaaattctg tataatgtgc cggggcgaac agggagtgat ttaaaacctg aaacaatcgg
     1501 tcgcttagcg aaaattgaga atattgttgc tgtgaaagaa gcaacgggtg atttaagccg
     1561 cttaccttta attaagcaat tagccggtga agattttatt ttcttaagtg gtgatgatgc
     1621 taccggtttt gagtctatga aattaggtgg tcaaggggtg atctcggtca ccaataatgt
     1681 tgctgctgct gatatggcaa aaatgtgtga gttagctctt gccggtcaat ttactgaagc
     1741 agaagaaatt aataaccgct taatggcgtt acaccatgac ttatttgtcg aatctaaccc
     1801 gattccggtg aaatgggctg cttataaatt aggtttaatt agtgagccta atttacgttt
     1861 accgcttacc acactgtctg aatcagcaca gcctgtagtg ttaaaagcat tacaacaagc
     1921 gggcttaatt tagttatttt ttgcaaatca aatttgaaac cggttaagtt aattctaacc
     1981 ggttttttca tatctaaaag aaatttccac ataaaaataa aattctaaat ttct
//
LOCUS       DANLUA010000260         2026 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLUA010000260 DANLUA010000000
VERSION     DANLUA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            38..466
                     /locus_tag="PXB44_002518"
     CDS             38..466
                     /locus_tag="PXB44_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL5282944.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            477..1085
                     /locus_tag="PXB44_002519"
     CDS             477..1085
                     /locus_tag="PXB44_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5282945.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(1147..1989)
                     /gene="cpdA"
                     /locus_tag="PXB44_002520"
     CDS             complement(1147..1989)
                     /gene="cpdA"
                     /locus_tag="PXB44_002520"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL5282946.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDNNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
BASE COUNT          614 a          406 c          414 g          592 t
ORIGIN      
        1 tttttgaaaa caagaatttt aatggagtga acctcaaatg agaaaatatc tcgccttcat
       61 tttagccgtg atgtccggct ttgcaatggc ggaagcccct aagttatccg gatcaattga
      121 tgagacaacc gaaagtgttg caccgggatt tcataaccca caaaaagatg tgggcacgat
      181 tccaaccact ttccctttcc aaccaccgct tgttccacat agtattcgtg gcttacaagt
      241 gagcaaaaac gtaaaccatt gtttaggttg ccacgctatt gaaccatcta aaaccacagg
      301 tgcgacccgt ttaccgccaa gccattttac cgaccgtgat ggcaaggtgt acgaaaacga
      361 atctcctcgt cgttatttct gtttgcaatg ccacgtgcaa caaactgatg taaacccgat
      421 tattcaaaat aaatttgaat ctgtacgtga atctcaagct aaataaggag taagtaatgt
      481 tgagaagatt ttgtaactgg ttacgtagcc caagcaaaat ggcaattggt gctgtaatcc
      541 ttatttctgc attaggtggt attttcgctt ggagtggttt taatgcaggg ttagcaatga
      601 caaatactga agagttttgt tcagattgcc atatgaacga tgttgtgccg gaataccgtg
      661 cctctgctca ctattcaaac cgcagcggtg tgaaagcgat ttgttcagat tgccacgtac
      721 cgcacgaatt ctttccaaaa tggcaacgta aaatcatagc cgcaaaagaa gtttatgcac
      781 actttaccgg taaagtagat accaaagaga aatttgaagc tcatcgtggc gaaatggcag
      841 aacgtgaatg ggcacgtatg aaagcgaaca gctctcaaga gtgccgtaac tgtcataatt
      901 ttgatgatat ggacttcact caacaaaaaa acattgcgca gcaaatgcac gcattggcac
      961 aagagcaagg taaaacctgt attgactgcc acaaagggat tgcacacaat ctcccacata
     1021 tggaagcgat ccagaaaaac tttatccctg atgatttgct gaaaaaagca gaagagaaga
     1081 aataacttaa cgtgtttcaa tagaaaaccc tcaatttcca ttgagggttt ctttttagta
     1141 gccaagttag taaccaatgg cattgacatt tggtaaaaaa tcgttggaat ttaaccgctt
     1201 gacgaccgtt tctatcgtgc cgttttcgtg caattcaatt tcacgccagc cttgtggttg
     1261 taagtctaag gtaaactggt cgcaattcgg cttaaattga atgcaagtag atggggtggc
     1321 ataaatcgga ataccatgcc aaatatcatc aacctgctga tgaatatgac catgcacaat
     1381 cgcttttaca ttcttaaatt ttgcgaaaat ctcggataat tcattgctgt tttttaagct
     1441 atgctgatcc agccacgctg agtttgtcgg caaaatattg tggtgcagag caaccaaggt
     1501 aaaccgttgc ggatattgtg ccaatttttc ttctaaccat ttaagctgtg agtaagagag
     1561 ttcgccataa ggcataccta ccacctgggt atttagcatc agaagctgcc attgctcacc
     1621 gagtaaaatt tgtttatggg gaaggatatg tttatattgc gccaaaattt ctgacatact
     1681 cggctgaaca tcgtgattcc cctctagcca cactatcggc gagtttagcg aattagttat
     1741 ctgtgcaaaa tagtgataac cagcaatatt gttatcttga atcaaatcac ctgtggcaag
     1801 aactagatca aaatattgct tcgcttgttt gtttatttgc tcaataactg ccgaaaaact
     1861 ttttactgtt ttgacattaa gtagagtttc ctccggattg gagaaaaggt gagggtcagt
     1921 tatttgtaat aacttaatta cccctgttac gggtaactta atatattcgt gcattcgttt
     1981 tgcatccatt caaattctag taatttttaa ctggtgagat tctatc
//
LOCUS       DANLUA010000261         2024 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLUA010000261 DANLUA010000000
VERSION     DANLUA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(24..1739)
                     /gene="proS"
                     /locus_tag="PXB44_002521"
     CDS             complement(24..1739)
                     /gene="proS"
                     /locus_tag="PXB44_002521"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL5282947.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
BASE COUNT          527 a          515 c          364 g          618 t
ORIGIN      
        1 acgcccaatt ttgttattta taattaagcc tttacattcg cttttaagaa cgcaagtaag
       61 ctctctttgg cgatcatctg tttttcaccg ctgcggcggt ttttgtattc gatttcgccg
      121 ttctcgaggt ttttctcacc aataaccacc atatgcggca caccgataag ctccatatcc
      181 gcgaacatca cgcccgggcg ttctttacga tcatcaaaaa tcacctcaac gccttgagct
      241 tttaagttag cgtataactc ttcagcgaat gcctgcactt tttccgattt gtgcatattc
      301 atcggcacaa tcgccaccgt aaatggcgcc atttcatcac aaggccagat aataccacga
      361 tcatcataat gttgttcaat cgcagctgcc accacacggg taacgccgat accgtaacaa
      421 cccattgtca tcactagcgg tttaccgtct tcaccttgca cagtggcttt cattgcttct
      481 gagtattttt tgcctaattg gaaaatatgc cctacttcaa tacctcgttt gatttgaagt
      541 gtgcctttac cgtccgggct tgggtcgcct tccaccacat tgcgtaaatc tgcaatttcc
      601 ggcacagcaa catcacgctc ccagttcacg ttaaagtagt gtttgccatc aatattcgca
      661 ccgcagccaa aatctgacat caacgccact gagcggtcaa taatcaccgg aatcggcata
      721 ttcaccacac ctaacgagcc aacaccggca ccgattttgg ctttaatttc cgcttcatcg
      781 gcaaactcaa gcggatcagc caccaacggg tgtttttgag ctttaatttc gtttaactcg
      841 tggtcgccac gaatcactaa cgcaattaac ggttgctctt cagttgcccc tttcaccact
      901 aaggttttca ccgttttttc aatggctaaa ccgtgatttt ctactaattc attgatggtt
      961 ttcgcatttg gcgtatccac taattgcatt tctgccgttg gtgcttggcg ttcacccacc
     1021 gcaaccgctt cggctaattc aatattggca gcaaaatctg attcggttga aaataccaca
     1081 tcatcttcac cgcttgaggc taacacttgg aactcgtgag aggctgaacc gccgatagag
     1141 ccggtatctg cctgcaccgc acggaaatct aagcccaaac gggtgaaaat attgctgtaa
     1201 acctgataca tcacatcata ggtttcttgc aggctctctt gtgtggtgtg gaaagaatag
     1261 gcatctttca tcacaaattc acgcgaacgc attaccccga aacgagggcg gacttcatca
     1321 cggaatttcg tttgaatttg gtataaattc agcggaagtt gtttgtacga agaaacttca
     1381 cggcgaacca agtcggtaat cacttcttcg tgagtcgggc caagcacgaa atcacgctcg
     1441 ccacgatctt taaaacgtaa taattccggg ccgtaatcat tccaacgacc ggattccacc
     1501 cataattccg ccggctgaac caccggcatt aatacctcga tagccccgcc tttgttcatc
     1561 tcttcacgaa taatattttc cacttttttc aacactttga tcccggtcgg caaccagttg
     1621 tataaacctg acgccatcgg gcgaatcatt ccggcacgca acattaattg atggctaact
     1681 acttgtgcat cattcggcat ttctttaagg gttgaaaata aatattgact tgtacgcatt
     1741 gctattccta ttctttttaa actttgaaac ctgaaaaaat ctgcttattt taaaacaaag
     1801 cggttaaatt ttcaggaaat tttgcaaaat cctataaaaa aataaccgct tgtaagcggt
     1861 tattttgttt taactaaggg tattttctta aactgtgtat gcttaatttt ctcgttcaat
     1921 ttgctcaata atctcttgcc attcggcatt tgcttgttca agagtaaaaa tctgaccttt
     1981 ttttacttta gcctctaaat attcatcata atctttttct ttaa
//
LOCUS       DANLUA010000262         2002 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLUA010000262 DANLUA010000000
VERSION     DANLUA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(10..1320)
                     /locus_tag="PXB44_002522"
     CDS             complement(10..1320)
                     /locus_tag="PXB44_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL5282948.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(1320..1622)
                     /locus_tag="PXB44_002523"
     CDS             complement(1320..1622)
                     /locus_tag="PXB44_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282949.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            complement(1663..1851)
                     /locus_tag="PXB44_002524"
     CDS             complement(1663..1851)
                     /locus_tag="PXB44_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282950.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
BASE COUNT          416 a          530 c          530 g          526 t
ORIGIN      
        1 gaaacagggc tataaacgcc ccgcctcaat ctcccgcacc agtgttgata tgtcagcgcc
       61 gtagttagtg gatggccccg cttcggcaac gccatagaac ggcatcctgc ccatagcgcc
      121 ctcgggtatc cgttgacgct gatagcgtgg cttcttgatc ttctggaagc cctcagtaaa
      181 caggatcttg gctcgcagcg cttcaaagct gtagccacgg ccaacacgat taacaacacg
      241 aatcaggttg ttcagtgact cggtgtaggc gttggtaatg gggtggtcga agtaagcgaa
      301 aatgtcatcg tgccagttgc ccatcgcttt gataagcggg tcatagtggg ctttcatttc
      361 cggcgtaatc tgtcgcagcc aagagtaata tgcctcctgt gcctggtgcc gggtctgaca
      421 gtcccaaatc tcgaaaaagg cttctttcag ttcgtaggct tggccaatct cagggtaatt
      481 caatgtccag cctgaaaaac gcatgtattc ggcatctgtc agctcgtgac ggcgtttcag
      541 taggacaaac ctatctcgca tcaacccacg gcgctgctgt ggcgtcaggg cgcttctgat
      601 ggcttttctg gcgcgttcca gtgattcgtt cgccatcctg acaacgtgga acttgtcgat
      661 gatcaccgtg gcgtcaggaa tcatggtttc tactgcttgc cggtacggcc tccacatatc
      721 cattgccacg tagcgcacca ggtcgcggtc agtgcgtttg ctcaggtagc gggtgacggt
      781 ggttttattg cggttatcca gcatgtccac aatggtctgc tgctgaatgt tggtgatcac
      841 gcagcggggt ttgatgatgt ggatttcgtc gatacccaac cactgcggca tttcaaaatt
      901 gagcgtcttt tcgaggcgtt cacaatagtc gttgaaaatg ttacggacgg tcttttcatc
      961 gacgcccaca tcctcggcaa cactggagaa cgtgcggcgc aggctctcgc gctcgatgta
     1021 ctggatcagg cggttggtca tctgccgctt ttcgtctttg tgcggtacag gctccagaaa
     1081 cgtctttcgg caagactggc agcgatagcg acggcggtta agcatgatgc cggtacgcct
     1141 tccgtggacg ggtgtatcca tgatcacctc atcacggcga ccaaagccga cgatttcagg
     1201 atgattgcag tgggggcact gcgtaggcgg ttcggaaact tcggcgtgta cctggtaatc
     1261 gtgctccagt tcggatacct ggagcacttt gtaatcaggc cagttgagga tattcgacat
     1321 tactcctttg ccaccttaac cgattcctcg gtgccagtgg cagcagtgac tagcgcgtta
     1381 tgaactcgac gactggtatc ttcactgtct gtttcaatcc acgcgccaaa ggcatgcacc
     1441 attgcagcaa aagacttccc atcaaggatt tcaccagact tgcctctgag caaagcgccc
     1501 ataccatagc tgcttgacgg atgatcggca gtccacctga gagtctcggg agacatttgg
     1561 cgaagcccca ttcgctttat cgtggcgata gactcgtccg gaaaatagag tttgattttc
     1621 atgctgacct cgctgaaagc cgcccctagg gcggcatgtt agtcaaattg cttcgccgcg
     1681 gcggatgttg cgaatacgaa aacggaggtg ctttgtcgct tggtgaaccc cttggccgcg
     1741 taattctgat tctttattcg ggttgttgtt gattaggtct tcagcaaccc tttcaagctc
     1801 gctaagcgcc tcttcgattt cattcagcgc atcatttgca tcgtatgaca tggcttctat
     1861 cctgcaccaa gttcggcggt gcgcccgttg ggcttatgcc cgagccgttg ttctcagctc
     1921 ttgaatctaa tattagggca tctggccttt tccgtcaacc ctaaaatccg gtttatttac
     1981 aaaattacgt ctagcgcaga tc
//
LOCUS       DANLUA010000263         1991 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLUA010000263 DANLUA010000000
VERSION     DANLUA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            41..688
                     /locus_tag="PXB44_002525"
     CDS             41..688
                     /locus_tag="PXB44_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5282951.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(766..1395)
                     /locus_tag="PXB44_002526"
     CDS             complement(766..1395)
                     /locus_tag="PXB44_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL5282952.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISMLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(1467..1940)
                     /locus_tag="PXB44_002527"
     CDS             complement(1467..1940)
                     /locus_tag="PXB44_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL5282953.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
BASE COUNT          620 a          374 c          369 g          628 t
ORIGIN      
        1 tgctttgtgt gagaagtttt agtaaaggga tagaaccaag atgattagta ttagaaatgt
       61 aagtaagcaa tatcgaacta ataacggttg gaaaaccata ttaaaagata taaattttga
      121 gctgaaaaaa ggcgaaaaag tggggatttt agggcgcaat ggtgcaggaa aatcaacctt
      181 aatccgttta atgagcggag tagaacctcc aacaacaggt acgattgaac gtcaaatgtc
      241 gatttcttgg ccactggcat ttagtggtgc attccagggt agtttgaccg gaatggataa
      301 tcttcgtttc gtcagccgaa tttatgatgc tgattttgac tatgtaaaat ctttcaccga
      361 agaattttca gaactcggtg aatatttata tgaaccggta aaaagctatt cttctggtat
      421 gaaggcccgt ttagcctttg cattatcgct ttctattgaa tttgattgtt acttaattga
      481 tgaagtgatt gcagtaggcg atgctcgatt cgctgccaaa tgtaagcacg agctttttga
      541 aaaacggaaa gaccgttcat tgattttagt ttctcatagc ccagcctcaa ttaaagaata
      601 ttgcgattct gcaatggttt tagaagaggg tatcatgcat acttttgatt ctatagataa
      661 cgcttataat ttctacaata aaacataaaa gaacaagcgg ttatatttca caagtttttc
      721 gcaaaaaact ctcaaaatat aaccgcttgt tactaaaact gaaaattaga aacctgcgtc
      781 tttttctaat tgttcaacta atgcctcagc taaacctaaa gcatcagcta aattagctaa
      841 gaaaacgatt tctttgcgtt ctaaatctct gcatactgca cgggaagcta agtaaacttg
      901 tgtagccaat gcttggtcat tacctacttg gcgggcaatc tcttcaactg tagccggagt
      961 gtccatttct tgacgaatcc actcaatgac ttcagcctca tctccaactt gggaaacaat
     1021 agctgctttt tcctcttcag taatagcacc atctgaagca gcagcggcaa tcattgcttg
     1081 taaaatcact ttacttgcca cattttcatc tacattttca aaattatttt ggctaagttc
     1141 tgaaaccgat gaattttgcg cttgatactt ttggtatgct tgataagcaa ggctaccgat
     1201 agcggctaaa gagcctaatt ttgctaaact tgaaccgcca ttacgcccaa ataacattga
     1261 aattaaacca atagcgacag cgccgccacc aattttagcg atttgatcgt ttttagaatt
     1321 acctttaatt aatccgtttg tagcggcatc ttttgctgta tctaatactt gacttaaaat
     1381 cttactgaaa tccatttttg ttcctcttgg taagtaaatg aaatttactc attagtcctt
     1441 agattcaaac aagagttcaa ttcaaattaa agataatttc cacaacagtg cttaaacttc
     1501 tttccggagc cacatacaca aggttgtttt tgatgaggca aaggtacggt aggatccaca
     1561 aaaaaccatc gctcatcaat ttgaacaaaa agagaggttt cgtggtgtcc ctgtatcccc
     1621 tcatctgtat caaaaaaggc tttaaattca acctgactat gtattctcga aagagaaggt
     1681 aagtgattta gcacggataa tcctacccat tttgtatttt cagcccactc tttcattgca
     1741 gtctgatcaa gtaatttctg ctgacttggc actgtcgttt ccacaatata gggaatatta
     1801 accaaagtat aagcactgta acgggagcgc attaattgct ctgccgttga tgcaaatgtc
     1861 tgttttaaat ggaaaggttg gcaacattca gcgtagcgtt tgcctgtttg gcaaggacaa
     1921 gcggtgattt tttctaacat ttttacattt ccgcataaaa ataaagatgt gaaaagttta
     1981 cgctataaat a
//
LOCUS       DANLUA010000264         1977 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLUA010000264 DANLUA010000000
VERSION     DANLUA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            99..740
                     /gene="sodA"
                     /locus_tag="PXB44_002528"
     CDS             99..740
                     /gene="sodA"
                     /locus_tag="PXB44_002528"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL5282954.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(803..1927)
                     /gene="alr"
                     /locus_tag="PXB44_002529"
     CDS             complement(803..1927)
                     /gene="alr"
                     /locus_tag="PXB44_002529"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL5282955.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVTAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
BASE COUNT          576 a          424 c          424 g          553 t
ORIGIN      
        1 ctagatcaaa aaaatgtgca ttttttgtgt gtttcttgat aaaatgtact cggagtttaa
       61 ctctgattaa cctatttact atttgagagg aattaactat ggcatataca ttaccagaat
      121 taggctacgc ttatgatgcg ttagagccac atttcgatgc taaaacaatg gaaatccacc
      181 attctaaaca tcaccaagct tacatcaaca atgcaaatgc agcattagaa gctcaccctg
      241 aattattaga aaaatgtccg ggtgcattaa tcaaagattt gagccaagtg cctgcagaaa
      301 aacgtattgc agtgcgtaac aacgtaggtg gtcatgtaaa ccacacttta ttctggaaag
      361 gtttaaaaac aggcactacc ttacaaggtg cattaaaaga agcgattgag cgtgatttcg
      421 gttcagttga agctttccaa tcagaatttg aaaaagcagc gactactcgt gttggttcag
      481 gctgggcgtg gttagtatta gaagagggta aattagccgt tgtttcaacc gctaaccagg
      541 acaacccact aatgggtaaa gaagtggcag gcgtttcagg ctatccgatt ttagtcttag
      601 acgtttggga acacgcttac tacttaaact accaaaaccg ccgtccggat ttcattaaag
      661 cattctggaa cgttgtaaac tgggacgaag ccgctcgccg ttttgaagaa aaagtagcaa
      721 cctgcggttg tgcgaaataa tattttcgat cttgaataag atacaagcgg taaaatttgt
      781 tgaaaatttt accgcttgtt gtctatttat attcaaaaat cacacgagga gtgagtttag
      841 taatcagctc atagctaatc acgccaatgt gtttcgccac atcttcaatt aatagttgct
      901 ctccccaaaa aatggcttta tcgcccactt tatcttggct ctctgcacct aaatcgaccg
      961 tcagcatatc catcgaaact cgaccgacaa tcggcactat tctaccgtta atcaacacag
     1021 gggtaccttc aggagcattg cgagggtagc catcgccgta gcccattgca gtcacgccga
     1081 gtttggtatc tttagggctt acccaagcac caccatagcc tacaggctcg ccggctttat
     1141 gggtacgcac agcgatcagg gaagaggaga gcgtcattac cggcttaaaa cctaaatcgg
     1201 taatcggcgt atagtgggga gaaatgccgt gcataataat acccgggcga acccaatcat
     1261 aatgagcctg tttccaataa agaataccgc ttgaggcgga aatactgcgg tcgtgatcct
     1321 gatatttttg cgtagcttgc tcaaacaccg caatttgttt ttcggtatag ccacaatcga
     1381 gttcatcggc acggctaaaa tgactcacaa agctgattga attgaccaag gcacaatttt
     1441 tcaaacgttc aacaaagtga tcgacttgct ccgggtgaat gcctaagcgg tgcattccgg
     1501 tgtcgatttt tagccaaatg ttaatcggga agtagatttt cgctttacga gtccaaaagc
     1561 ctttttgttg ctctgcctgc cattcttttg ctacttgctc gagtaattcc agttgttcga
     1621 ggttatgaat gacggtatca aacttgcggg aaagggtttt gagtagctct tctcggtcaa
     1681 aaaaaccttc aactaacaca attttgcctg tgtatccggt ttcttgaatt tctaaggctt
     1741 cacgcaaacg ggcaactcca aagccatcaa ccatatctgc taatgtgccg gcagccggca
     1801 ataagccttg cccgtaagca ttggctttga tcatcgccaa taatttttgc tgtggagcga
     1861 aagatttgat caggcgaata ttatggcgga gggcttgact atcaatggtt gctgttgctg
     1921 gtttcatact tatctacata gttaaaatca caagaaaggc taaattatac gcctaaa
//
LOCUS       DANLUA010000265         1869 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLUA010000265 DANLUA010000000
VERSION     DANLUA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            158..1108
                     /gene="coaA"
                     /locus_tag="PXB44_002530"
     CDS             158..1108
                     /gene="coaA"
                     /locus_tag="PXB44_002530"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL5282956.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1261..1800
                     /locus_tag="PXB44_002531"
     CDS             1261..1800
                     /locus_tag="PXB44_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL5282957.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          591 a          385 c          332 g          561 t
ORIGIN      
        1 tttcgatttt taaatataca aaatgactat tttttgtaaa atacaacgga ttttctctgc
       61 ataactacgg caaaataagt gcatatattg tatagttacg cccttaattt ggtatttaat
      121 taccttaata ttccagcaaa aaattgagat tttggctatg agccttgaaa aaaccagcaa
      181 aattactcct tttctcactt ttgatcgcca acaatgggct gatttacgca aatccgttcc
      241 attgaagctg accgagcaag acctaaaacc gcttttaggc tttaatgaag atctttcatt
      301 agaagaagta cgcactattt atttaccgct tgcccgttta attcaatact atatagaaga
      361 aaacttaaaa cgccagacag tcttacatcg ctttctcggc attgaaccgc caaaagtccc
      421 ttacattatt agtattgccg gcagcgtatc ggttgggaaa agtacctctg cccgtattct
      481 gcaagctctg ctctcccatt accctaaaga gcgaaaagtg gatttaatta ccaccgacgg
      541 ttttttatat ccacttgcaa cgctaaccga aaagaactta ctcaagaaaa aaggctttcc
      601 ggaatcttat gatattcaca gcctgattcg ctttgtatct gatattaaat ccggcaaacg
      661 caatgtgaaa gctccgattt attcacattt gacctacgat attatcccaa atcaatttaa
      721 tacggtggat caacccgata ttgtgatttt agaagggcta aacgtgttac aaagtggaat
      781 gaactacccg tccagcccac ataatgtatt tgtatcggac tttgtggatt tttctattta
      841 tgtggatgcc gaagaaagtt tgctgaaaaa atggtatatc aaccgtttct tgaaattccg
      901 ccgcagtgcc tttgccgacc caaactccta tttccaccat tatgcgagtt tgtccgagca
      961 agaagcgatt gaaaccgcct caacgatttg ggaagagatc aacggcttaa atttacgcca
     1021 aaatattctc ccaagccgag agcgtgccaa cctcatttta atcaaagggc aagaccacgc
     1081 cattgaaacg gtaaaattac gcaaataata ttttgcaaat aattgggctt tttcgaccgc
     1141 ttgtaatacc ttaaaatccg ttgaatacca ctcaacggat tttttattta aatcaatgct
     1201 tattcaacta ttttttggta agctaacgac tttgagagtg attgatatta ggcatataca
     1261 atgaacaaaa aatttctact tcctttaatt ctctttttgg cgttatctgt ggcatttttg
     1321 gttcaactcg ctcgcaactc gcaaggcgat gatccgaaaa aattagaatc agccttaatt
     1381 ggcaaaacag ttccgagctt taatttagaa tcggtattag atgaaaaaca gacaatcgac
     1441 caaaatatcg tcaaaaccgg caaacctcgc ctgcttaacg tgtgggcaac ttggtgtccg
     1501 acttgttatg ccgaacacga atacttaacc attttggcaa aacaaggggt ggaaattgtc
     1561 ggtattgatt ataaagacga acgcccaaaa gcgattaaat tcttagccaa ttacggcaac
     1621 ccttataaag cgattatttt tgacccgaaa ggctcactcg gtttagattt aggtgtttat
     1681 ggtgcacccg aaacctttat tattgacggc aacggcgtga ttcaataccg ccacgccggc
     1741 gatgtaaacg accaagtgtg gaaccaagtg cttaaaccga tttatgatgg attaaagtaa
     1801 atgtttgtat ttcctgctga cattaagaaa tctataaaaa gcaattatca aagagaatat
     1861 aactttatt
//
LOCUS       DANLUA010000266         1867 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLUA010000266 DANLUA010000000
VERSION     DANLUA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            38..1513
                     /gene="mepM"
                     /locus_tag="PXB44_002532"
     CDS             38..1513
                     /gene="mepM"
                     /locus_tag="PXB44_002532"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL5282958.1"
                     /translation="MAVFLLAVFSLFVDIVLALKDQSSINNTSSQEDIIAIDKQEKYQ
                     SINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDEIVDDQTATSYEDELVGQD
                     DEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRIQNQFSYTVARGDKLKDVL
                     EQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELEYMNWLVSEKEERVYERKE
                     NGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDRQINQLAVGLQSQIATNKL
                     KKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAIQADNGRYYSRYGETLSGG
                     FARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPIGTPIIAPSDGVVEHVAYQ
                     ARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIALSGNTGGSTGPHLHYEFH
                     INGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLKM"
     gene            1523..>1867
                     /locus_tag="PXB44_002533"
     CDS             1523..>1867
                     /locus_tag="PXB44_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249160.1"
                     /note="DsbC; periplasmic protein with protein disulfide
                     isomerase activity; this protein also shows disulfide
                     oxidoreductase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL5282959.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDI"
BASE COUNT          662 a          288 c          406 g          511 t
ORIGIN      
        1 agcgagagat cgtaagcgac ataaaaaaca ttttaagatt gcggttttct tattagcggt
       61 atttagtcta tttgttgata ttgttctcgc actaaaagat caatcctcaa tcaataatac
      121 atcttcacag gaagatatta ttgccattga taaacaggaa aaatatcaga gtatcaacac
      181 taatttggcg gaaaataata ctctccatca ggagcaacat tccagtttta ttaattatgt
      241 gactgattta ctgactcagt cggcattagt aatcgaacat gacgacgaaa ttgttgatga
      301 tcaaacagcg acttcttatg aagatgaatt agttgggcaa gacgatgaag tagaagaagg
      361 gattgaaacg gttgaaaatc aagttcctgg ggaaaaactc ccagaagaag ctgaaaaggc
      421 attagaagat ttacttgatg tggctgatca agcactacga attcaaaacc aattcagtta
      481 taccgtagct cgaggtgata aacttaaaga tgtattagag caatcaggct taactgcatc
      541 cgttgcgaaa aatttaatta agcaacattc ggcattagca caattagaac cgggtcaaca
      601 attttattgg gtattagata atgacggtga attagaatat atgaactggc tggtttccga
      661 aaaagaggaa cgggtttatg agcgaaaaga aaatggtaaa ttttcttttc aaagatttga
      721 gaaaaaaggg gaatggaagt tagatgttgt aagaggaact attcagaata gttttgcggt
      781 tagcttaaaa gccgttggct tatcagaccg ccaaattaac cagctagcgg ttggattgca
      841 gtcacaaatt gcgacgaata aattaaaaaa aggggatagg tttgcaattc tgattaagcg
      901 ggaatatatt gacggcaaag taacggattt aggaaatgtg gaaggaatcc atgtaattag
      961 cagtaaaaaa agttattatg ctattcaagc agataatgga cgctattata gtcgatatgg
     1021 tgaaacatta agtggaggat ttgctcgtca tccattatta tttgcagcaa gagtaacttc
     1081 ttctttcaac cccaaacgca gacaccctat tacagggaga attagtccgc ataaaggggt
     1141 tgactatggc gtgccaatag gtactccgat catcgcacca agtgatggtg tcgtagagca
     1201 tgtggcatat caagcccgtg gagcaggacg ttatattcgg ataaaacacg gacatattac
     1261 gactgtttat atgcacctca gcaaaacgtt ggtaaaacca gggcagagtg ttaaaaaagg
     1321 ggagcggata gcattatcgg gtaatacagg cggttcaacc gggcctcatt tacactatga
     1381 attccatatt aatggtcgac cggtcaaccc gattacggta aaattgcctg gctcaaattc
     1441 aggtatgggt aataaagaac gtcaagcatt cttaaataaa gcgaagaatg tagaaagtag
     1501 attaaaaatg taaggattct caatgaaaaa aaatctaatc actttaggat taatgtgttt
     1561 atcaaccatc acgtttgctt cagatgcaaa actaaaaagc cagcttgaaa aaattggagc
     1621 cacagatgtt aaaatttcag attctccatt aacaggcttt aaaatggcga cttcaaatga
     1681 aggtgttgta tatatcagcc aagacggtcg ttatgtaatt caaggtaaag tatttgagct
     1741 aaaaaatggt aaagcgattg atattactaa tcaaggattg ttggcagaat tgaattcata
     1801 cactaatgaa atgatcgtat atccagcgaa aaatgagaaa catgtgatta cggtgtttat
     1861 ggatatt
//
LOCUS       DANLUA010000267         1779 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLUA010000267 DANLUA010000000
VERSION     DANLUA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..493
                     /locus_tag="PXB44_002534"
     CDS             <1..493
                     /locus_tag="PXB44_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282960.1"
                     /translation="ARPLNTLEVKGLTVVDDSQKPLFSEVNQALYHGLSPIEVVASRV
                     QITRAITTYTKNVTNTDDPSYLDLTTIRTLDYVRKAIQTRQRLRFPRAKNSHRIVAKV
                     RSEILDVLYQLEGEEVIENVEAWKNRLLVVRNQDPTYLDLEIPADVVNGLHVIRNKIT
                     LIL"
     gene            504..878
                     /locus_tag="PXB44_002535"
     CDS             504..878
                     /locus_tag="PXB44_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5282961.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            878..1246
                     /locus_tag="PXB44_002536"
     CDS             878..1246
                     /locus_tag="PXB44_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282962.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            1276..1464
                     /locus_tag="PXB44_002537"
     CDS             1276..1464
                     /locus_tag="PXB44_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282963.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            1517..>1779
                     /locus_tag="PXB44_002538"
     CDS             1517..>1779
                     /locus_tag="PXB44_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282964.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVR"
BASE COUNT          540 a          352 c          447 g          440 t
ORIGIN      
        1 ggctcgcccg ttaaataccc ttgaagttaa aggcttaacg gtcgtagatg actcgcagaa
       61 accgttattt agcgaagtaa accaagcgtt atatcacggt ttaagcccga ttgaggtggt
      121 ggcaagccgt gtgcagatta cccgtgcgat tactacatac accaaaaacg tgacgaatac
      181 tgatgaccca agctatttgg atttaaccac cattcgtacg cttgactatg tgcgtaaggc
      241 aatccaaacc cgtcagcgtt tgcgtttccc tcgtgcgaaa aactcccacc gtattgtggc
      301 aaaagtacgc tcggagattt tagatgtgct ttatcagctt gagggcgaag aggtaatcga
      361 aaatgtggaa gcgtggaaaa atcgcttatt agtggtgcgt aatcaagacc cgacttactt
      421 ggatttagaa atccctgcgg atgtggttaa tggcttgcac gttattcgta acaaaattac
      481 gttaatttta tagggggtgc tagatgtcaa ttgtttatga aggctcttgc gtgcttgagg
      541 tagatggcgt tgagattgaa attaccaaac ttgatgtaaa aaccgtaacc ggtcgcaagc
      601 tggttaaaac gatgaacagt tccggacgtg caagaggtta ttcgcaaggc attgcggaat
      661 atactctctc ggtgactgca gttgagcctc aagacggttc ggtaattgat tgggataaca
      721 ttaaaaatgc gaagctcact aaatacccgt tagacaatag cgaaaaacgc acctcttatt
      781 taggctgttt tactaccgat gtgagcgagt cttacaccgt tgatggtgag tcggtgcgtg
      841 atattcaact caacgcattg cgtaaggtga atgaataatg aaaatcactt taaatcttgg
      901 cattgaatat aacgggaaaa ttcgccgtga ttgtacggtg cgattgctga cgatgggcgg
      961 tcaatgcacg gctcaagaaa tgattgcgga tttagggttt gaggaaaact cggaagccct
     1021 atcaaagcgt gaacaaacct tggtcgatat ggcttacctt tcgcagcaag ttacctttga
     1081 tggtatttat caaggtaatg tgacggctca atttttattt gacaatttag ctaccgaaga
     1141 ctactggcag ttgattgagg caacgctcac actccgaaaa aagcggttag aaaatggggt
     1201 gagccaaagc gagagcgaaa gcccggcaaa tcccgatttg gcgtagcaga agcctttaag
     1261 cagtaccgac aagcggtgat tttactctca aaatttgcaa ttaccgccga gcaggtatgg
     1321 caaatgccgt tgcttgaggt atttgcgtgg attgagagct acttggagag tgagggcgta
     1381 aaaacaccgc atagcccaat gggtgcgaat agcgaagtga aacgcgaaag ctatgtcttt
     1441 actcggtgtg gcaaaattga ataggggcga aagccccttt tttgtacttt aaaatgagtt
     1501 taaagagact ttaaaaatgg caaaaaatat gaaagtgcaa ctggaattga cggcaaaaga
     1561 tactgcctcc ccagttttaa accaagccgc taaaaccgcc gaaaaagcct ttaaaaatac
     1621 agagcgagcg gcacaaaaaa gtggcaatgc tcaaacgcaa gcagcacaga aagcagcgaa
     1681 tgctacagaa agctcaaatc ggaaaataga acagagttac cgtaatgccc gccgagtggc
     1741 tgccgatgtg agccgagccc gtgaaacatt aggtgttcg
//
LOCUS       DANLUA010000268         1704 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLUA010000268 DANLUA010000000
VERSION     DANLUA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            428..907
                     /locus_tag="PXB44_002539"
     CDS             428..907
                     /locus_tag="PXB44_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL5282965.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
     gene            876..1640
                     /locus_tag="PXB44_002540"
     CDS             876..1640
                     /locus_tag="PXB44_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5282966.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
BASE COUNT          578 a          328 c          340 g          458 t
ORIGIN      
        1 tagttgctga taatagaata gaaatgcaac taggaaagac aaaccaacct agccgcacaa
       61 atgaacattt tataaacgaa aataaatagc ttttatgtaa atgatcccta aaatgatccc
      121 tatttttgta gagtttattt tatctcttta taaatcaata agttataagc gagttcagag
      181 gaggctggga ccaacttaaa ttttccactc ttatttcttc attcgcattc ttacgcaaaa
      241 ccattgatga tactggcttt agagcaattt taaattattt cttcattcgt attctttcgt
      301 gttcttgcgt ttacatcccc ttattttaag ggtatatttg agggtataac gtaaaagggt
      361 atgaaaccca tacccttaaa tcccttatga ttctgataaa gcacaatgcg attttaggta
      421 aatttttatg gctagagtta ccacagaatt aaataacact caaatcaaaa acgcaaaggc
      481 tcaaaccaaa gactacaagc taatggacgg caaggggctg tttttgctgg ttaagaaaaa
      541 cggctcgaag ttatggcggt ttaggtataa aaagccgatt accggcaaag agagcgattt
      601 aggcataggc agttaccccg aaactaccct agcccaagca agaacgatta gagaagaata
      661 tagggcatta ctagcggaaa atatcgaccc actcgaacac cgccaaagat tagaggctga
      721 agcgttagcc gaaatagaaa acacctttta taaagtctgc ttgaaatggc gtgataaatg
      781 cgaaagccgt gtaaaaaatg gcgaacttca agccttaacg ctgaagaaga attggcgaat
      841 ccttgaaaag tatcttttgc cttgccgact actctattcc aaacatcacg ccaaaactag
      901 ttattaatgc tttagagcct gctaaagaaa aaggcatagg cgacaccctt aaacgagcaa
      961 ttaggctact taatgaagtg atgaattttg cggtaaatgc ggacgttatc gagtttaaca
     1021 aatgccaaaa tgtaggctca aattttagcg tagcgaaatc tgaacataac ccaacgatac
     1081 acccaaaaga gctacctcaa tttttagctg cattgcgtga ttacaaccta tcatttcaaa
     1141 ctaagatgtt gattaaatgg caactattaa caatgacacg ctcacgagag gcaagcgaaa
     1201 cgttgtggag tgaaattgat ttagaggcga aaacgtggac catttcagcc gaaagaatga
     1261 agatgaaacg ccctcacgtt atccctcttt cacggcaagc ggtcgaaatt ttagaaagaa
     1321 tgcgaaaaat cacagggaat agcccatttg tttttcaaag cgaaatgaaa ccacgccaac
     1381 cgatgaacag ccaaaccgcc aacagagcca taggcttatt aggctatgcc ggcactctaa
     1441 cggctcacgg tatgcggtct attgcctcaa cctatttgaa tgagcagtta gtcaattatg
     1501 atgtagtaga ggcttgttta gctcacgtta tcaaagacca aacccgaaag gcttataacc
     1561 gttctgatta tttagatcag cgtgtagatg taatgcagca atgggcggat tatgtagaaa
     1621 gctgctcaac ggggatttag taaatttttg gcggaatttg accgcttgtt taattagagg
     1681 tgtacaaata tgaataacct ttct
//
LOCUS       DANLUA010000269         1700 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLUA010000269 DANLUA010000000
VERSION     DANLUA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>1700
                     /locus_tag="PXB44_002541"
     rRNA            <1..>1700
                     /locus_tag="PXB44_002541"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          508 a          330 c          505 g          357 t
ORIGIN      
        1 agtcgataag aggcgtttaa tccaagatgt ccgaatgggg aaacccagta ggtgaagaac
       61 ctactatcac ccagtgaata catagctgtg tgaggcaaac cgggagaact gaaacatcta
      121 agtaccccga ggaaaagaaa tcaaccgaga tttcgttagt agcggcgagc gaacgcgaaa
      181 gagccagtta gtgatagcag taaagttagg ggaatgttct gggaaggaca atcgtagagg
      241 gtgataatcc cgtaccttaa aactccattg tggtactagg ctaacaacaa gtagggcggg
      301 acacgtgata tcctgtttga agatgggggg accatcctcc aaggctaaat actcctgatt
      361 gaccgatagt gaaccagtac tgtgaaggaa aggtgaaaag aaccccggcg aggggagtga
      421 aatagaacct gaaaccttgt gcgtacaagc agtgggagca agagtaatct tgtgactgcg
      481 taccttttgt ataatgggtc agcgacttat attttgtagc aaggttaact gaataaggga
      541 gccgtaggga aaccgagtct taactgggcg tgtagttgca aggtatagac ccgaaacccg
      601 gtgatctagc catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact
      661 aatgttgaaa aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag
      721 atagctggtt ctccccgaaa tctatttagg tagagccttg agcggacacc ttcgggggta
      781 gagcactgtt tcggctaggg gtccatcccg gattaccaac ccgatgcaaa ctgcgaatac
      841 cgaagagtga tactcaggag acacacggtg ggtgctaacg tccatcgtgg agagggaaac
      901 aacccagacc gccagctaag gtcccaaagt ctatattaag tgggaaacga agtgggaagg
      961 cttagacagc taggatgttg gcttagaagc agccaccatt taaagaaagc gtaatagctc
     1021 actagtcgag tcggcctgcg cggaagatgt aacggggctc aaatatagca ccgaagctgc
     1081 ggcatcagac gaaagtctgt tgggtagggg agcgttgtgt aagtcgatga aggtggattg
     1141 agaagtctgc tggagatatc acaagtgcga atgctgacat aagtaacgat aaaacgagtg
     1201 aaaaactcgt tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag
     1261 tcggccccta aggcgaggct gaaaagcgta gtcgatggga aacgggttaa tattcccgta
     1321 cttgtaataa ctgcgatgtg gggacggaga aggttaggtt agcagactgt tggatgtctg
     1381 tttaagccgg taggtgggaa gattaggcaa atccggtctt ccttaacacc gagaagtgat
     1441 gacgagtctc tacggagatg aagtaatcga taccacgctt ccaggaaaag ccactaagct
     1501 tcaggttatt ataaaccgta ctataaaccg acacaggtgg tcaggtagag aatactcagg
     1561 cgcttgagag aactcgggtg aaggaactag gcaaaatagc accgtaactt cgggagaagg
     1621 tgcgccggcg tagattgtag tcccttgcgg atgaaggttg aaccggtcga agataccagc
     1681 tggctgcaac tgtttattaa
//
LOCUS       DANLUA010000270         1698 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLUA010000270 DANLUA010000000
VERSION     DANLUA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            132..>1698
                     /locus_tag="PXB44_002542"
     CDS             132..>1698
                     /locus_tag="PXB44_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL5282967.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTN"
BASE COUNT          542 a          309 c          339 g          508 t
ORIGIN      
        1 ttattttttt gtgcttaaat cttaagcata aaatgccgtt ataattactt gctatctggt
       61 attttataca taagattgaa ccatataata gctgttcagc taaaaattag tctttgcact
      121 ttatccccct aatggagaaa atgatgagaa aaatcaatca gttaatcata tccccatact
      181 ttttcctaag tattttatct gcttccgcta actctgattt ttggaaaagc gaccttaatt
      241 ttaatctcac aaatattacc aagcgtgtcg gagtcgataa ctttaccact ccagtagctg
      301 gcgagccttg ggctggtatt ggtccaaatg gtgaagctgc aggtactgcg ccattatatt
      361 atagccgtat tccagctatg caagttaccg aagataacaa attagttgtt atgtttgatt
      421 tacgctggaa tcgtgcgtat gaccaagatc gtattgatcc tggtattgcg atttcttccg
      481 atggtggcca tacttggacg aagaaaaccg cttggtcttt tgatagaact aatcacccag
      541 cacgccgttc aatggatcca acgctattac ataatcctat tgataatagt ttatatgtaa
      601 tgcacggaac ttggtctatg tcggatcaac aatggtatgg tgggcgtgtt cctcacttta
      661 atagtggcag ctgggcagct accatttata aatctactga tggtggttta aattgggaga
      721 aaaatactga gtttagtaaa ttcagcaatt tagacgtatt ttctaaagtc accaaaaatg
      781 gaaaacctac cttaggcttt ttaggcgggg tcggatctgg aattgtaatg caagatggta
      841 cgttggtttt cccaattcaa accgcacatc aaaatggtat tgcgaccact attatgtatt
      901 ctaaggataa tgggaaaact tgggaaatgc cagaaattga caacccagtt gcaccgaacc
      961 aaagttcttt agaaaatatg gtgtttgaac tcgaaccggg taagctagtg atgactggac
     1021 gaggaaacag tcgctgggca tactcttcta ctgatatggg taagacctgg gaattattta
     1081 ctccaactaa tggattatta ccaactagct cacaaccaac tcaaggctct tctatctatg
     1141 taacactacc aaatggtcga aaagtgctac taatctctaa accagatgga aagaatgatg
     1201 ggtggaaaag gggtgatatt acactttgga tgctagatgc aaaagatcca tcgcacaaac
     1261 acaaggtgca tattattcgt ccaggttcag gtaatgccgc tggtgctggt tattcctctt
     1321 tagcctataa agaagggaat ttatttgttg cttttgaaaa cgatggtgat atttcagtaa
     1381 aaaatttaac tgaacatatt gctgaaatgg aagcaaaagc tatcgaatgg ggattaccag
     1441 atgaacttac tcctgctttg gataaaatca atgcactgcc ttatctcaat aaagctcaaa
     1501 aagagacgct agtcgaaaaa atgcgtcgtg ctaatgatac tgcgattgca caatcttttg
     1561 tgattaacaa agaaatgtat aatctgaaaa acaatagtga tgagctggat aaattggcaa
     1621 aaaatattac taaagcactg ccgtctcaac agaatattta tcaaagagcc ttagcctatg
     1681 tgaataaagt caccaata
//
LOCUS       DANLUA010000271         1693 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLUA010000271 DANLUA010000000
VERSION     DANLUA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            94..474
                     /locus_tag="PXB44_002543"
     CDS             94..474
                     /locus_tag="PXB44_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282968.1"
                     /translation="MKFIDFGKQLAKPVKFIKQRTAFLPKPLQQAVVFAVIFAGLPLF
                     LVGFTVLRILNGTPVEDISTNDVAYNPNHVENTVNEIEDDSEDSKPEDLWDVIADQDE
                     NQLDGYRDGYFGYGLYQGGYFVKR"
     gene            complement(527..>1693)
                     /locus_tag="PXB44_002544"
     CDS             complement(527..>1693)
                     /locus_tag="PXB44_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5282969.1"
                     /translation="QMNSELGNQQARVPLWWGLFHQINKGVTHALTASIPCKPDLRQV
                     RFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQSSQLSLTPAQVRETSWLGGRILLT
                     NSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANTGLKDRLLADVK
                     QNFSAKVIDFFSSKENADESILRTVLRAENMDISRGKVYPGYGGNLDPTFANATTRLT
                     SSVGSLAASFAAFPAFDAMRQALPMVHAFMLMAVIIITPLVIVMSGYSLKTLVTLTFV
                     SFALVSLTFWWELARWLDSWLLSALYDSSAHSRINPYFLENTQDDFIVNFVMGSMFLV
                     LPAIWLGSMSWAGVKIGDIAAGFTTDSLDSKSAGSQGGNLVKKGGKEISAGFNK"
BASE COUNT          539 a          347 c          318 g          489 t
ORIGIN      
        1 aaaagtttta tcttattctg taaaagacaa taaagataaa gtaatttata gtagtcgcca
       61 aaaacaataa ccttttatct taggaaagat actatgaaat ttatcgattt cggaaagcag
      121 ctcgctaagc cggttaaatt tatcaaacaa cgtaccgcat ttttaccaaa accgctacaa
      181 caagctgtcg tttttgcggt gatttttgca ggacttccct tgtttttagt tggattcacg
      241 gttctgcgaa ttctcaatgg aacaccagta gaagatattt caacaaatga cgtagcatat
      301 aatccaaacc acgtcgaaaa cacggttaat gaaattgaag atgatagtga ggatagtaag
      361 ccggaagatt tatgggatgt aattgctgat caagacgaaa atcagcttga tggatatcgt
      421 gatggatatt ttggatatgg actctatcag ggtggctatt ttgtaaaaag atgagaaaat
      481 atgattagaa attaataagg tagagattaa cctctacctt attgttttat ttgttaaacc
      541 ctgcagatat ttccttaccg cctttcttaa ctaaattacc accttgagag cctgcggatt
      601 tactatctaa tgaatcagta gtaaaaccgg cagctatatc tcctattttt actccagccc
      661 acgacatact gcctagccaa atcgccggta aaaccaagaa catcgaaccc atcacaaaat
      721 tgacgataaa atcatcttgc gtattttcaa ggaaatacgg atttattcga ctatgtgcac
      781 tggaatcata tagagcggat agtagccaag aatctaacca tcttgctaat tcccaccaga
      841 atgtcagtga taccaaagca aaactcacaa aagttaatgt aactaatgtt ttgagagaat
      901 aaccactcat cacaataacc agcggagtaa tgatgatcac agccatcaac ataaaagcat
      961 gtaccatcgg cagtgcttgt cgcattgcat cgaatgctgg aaaggctgca aagctggcag
     1021 ctaagcttcc cactgaagat gttaatcgag tcgttgcatt tgcaaatgta ggatctaaat
     1081 tccccccata cccgggataa actttccctc tcgaaatatc catattctct gctcgtaata
     1141 cagtacggag tatggattca tccgcatttt ctttgctgct aaaaaaatca atcacttttg
     1201 cagagaagtt ctgtttgaca tcagctaata aacgatcttt taaaccagta ttcgccgtat
     1261 cccaccactc tgcacaattt ggaaaaccac cttccccatt attcggcaat ccattatccc
     1321 gggaagcatt gtagggaaat agactatgag gttgttgagc tcgataacgg ggataaagct
     1381 cactgtttgt caaaagaata cgaccaccta accagctggt ttcacgcacc tgtgccggag
     1441 tcaatgagag ctgactgctt tgaattttct gacgtgcagg tacataacat tgttgtacaa
     1501 ataagcgaac ctcagtcaat aatgcaggat tattgatttt ttcatgttgg acctcaaagc
     1561 gaacttgacg taaatccggt ttacacggaa ttgaagctgt taatgcgtgt gtaactcctt
     1621 tgttgatttg atggaataat ccccaccaaa gcggtactct cgcttgctgg ttgcccaatt
     1681 cagagttcat ttg
//
LOCUS       DANLUA010000272         1643 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLUA010000272 DANLUA010000000
VERSION     DANLUA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..226
                     /locus_tag="PXB44_002545"
     CDS             <1..226
                     /locus_tag="PXB44_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282970.1"
                     /translation="KQNKNLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQL
                     ASFEQKSPMALHREKFEAKPTGLAELINNL"
     gene            237..1187
                     /locus_tag="PXB44_002546"
     CDS             237..1187
                     /locus_tag="PXB44_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL5282971.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1184..1378
                     /locus_tag="PXB44_002547"
     CDS             1184..1378
                     /locus_tag="PXB44_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282972.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
     gene            1496..>1643
                     /locus_tag="PXB44_002548"
     CDS             1496..>1643
                     /locus_tag="PXB44_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282973.1"
                     /translation="MTNYKLPARKWYTLEQATKRIKQLTGEDIEIEDLLHYWYLRKLE
                     LSIYL"
BASE COUNT          617 a          331 c          309 g          386 t
ORIGIN      
        1 caaacaaaat aaaaacctca cagatgaaag tgaagagcaa gctattctgg catatctaac
       61 agaaaatata atggctagaa ttgaaaagga acagccactt gaaaaaacat taattcttta
      121 ttctgaacag ttagcaagtt ttgaacaaaa atcgccaatg gcattacacc gtgaaaaatt
      181 tgaagcaaaa cctaccggct tagctgaatt aattaataac ttataagagg taaaaaatga
      241 gtttatccac tttcgcccac gcattaaaac tagataaaca aggtaacgtt attttatttg
      301 acgatagccc cgaagaacag gcaaaactaa ttagccgctt aaaacaaaat cgccttttta
      361 aattattgaa tttagtgcaa accaagcacg caaagggcga agcgattggg ctattcacac
      421 cgatagccgg cacaaccaac accgaaagcg gagaggaacg agagccaaaa caagcccttg
      481 cacccgctca aaaataccac tgcgaacaaa tcaacattga tagctatatt cgctatgaaa
      541 aaatcgatgc ttacaacctc gaaaacagct tagaagaacg cttaaatcag ttacttgatg
      601 acaaccttct aagagggctt ttaatggttg gctggcaagg cgtaagccgt gcagatacat
      661 caaacgcgac aaacaaccaa ctcgcccaag acgtgaaaaa aggctggcta caaaaaatca
      721 gagagggcaa gcccgaagcg gtaattaatg gagcaaccat aggcaaagag caacaataca
      781 aaagcctaaa tgctctgatt aagcagggat taggcaaaat tagcccagcg tatgcaatgg
      841 gcggagatat gatcgccatt tgtgggcggg atattatcgc tgatgatgta attcagcttg
      901 aacacaacga tttaagcgaa aacctagccg acttattaac cttatgccaa caagctaaag
      961 gcggactaaa agcggtaagt attccctact tcccaagcaa tgcgatttta atcactcgat
     1021 tagataacct ttcgctatat cttcatttaa acaatatccg ccgtaaagcg tttgatacgc
     1081 cacaaaaaga cagcgtagag acattttact ccctcaatgt ggattacatt gtagaagatt
     1141 ttaacgcctg ttgtttaatt gaaaatattg aggttattga agaatgagcc tagaaagcta
     1201 tatccaacaa gcggaagaaa tggagctttt agaaaaactc gattttgttt cagacctgtt
     1261 agaacttgag aaagtcagcg aaattgaagc ctatcgcatt ttaaccgccc acggcgaacc
     1321 taaaccccaa ccggcacaag aaaagctaat aggttgcgta gattttcttg atgactaacc
     1381 caagccccga aaggggcttt ttgcattttt gcaacaaaaa acgaccgctt gcaccctttg
     1441 aaattattgc tatcattaat aaaaaacaat aaccaaatca aatgagagta cagcaatgac
     1501 aaactataaa ctaccggcta gaaagtggta caccttagag caggcaacaa aacgcattaa
     1561 acaactcact ggcgaagata tagaaataga agatttatta cattattggt atttaagaaa
     1621 gctagaatta agtatttatt taa
//
LOCUS       DANLUA010000273         1554 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLUA010000273 DANLUA010000000
VERSION     DANLUA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            12..335
                     /locus_tag="PXB44_002549"
     CDS             12..335
                     /locus_tag="PXB44_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5282974.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(412..1430)
                     /gene="glpX"
                     /locus_tag="PXB44_002550"
                     /pseudo
     CDS             complement(412..1430)
                     /gene="glpX"
                     /locus_tag="PXB44_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT          491 a          363 c          274 g          426 t
ORIGIN      
        1 tcaaaaataa aatgaaacta tcaagcgaac tacaaaatca gataaaccaa ctaaatcagt
       61 tacgaaaaca ggcactcaaa caagagcaag acgagcagaa attgtttgaa aaaaataaaa
      121 ttgaagattt gccacatttt ggcacgcagc ttaatgctca gaggaaacta ttaggcatag
      181 aattaaccac ccttgaaatg caaaccggca tttcaagctc taccctaaaa cgcttattta
      241 aagacccaag ccaagtgaaa tttcagacaa tttgtattgt ggcagaaaca ctcggcttta
      301 atttatgtgc aattaatact cacaaacaag agtaagcgtg acaatgtagt gattttgatt
      361 actttgggaa gacaaaaaat cgggctaaaa gcccgattta tcagagaaaa gctataaatc
      421 caataaagaa aggagttcgt tatctttacg atcgacataa tggtcggatt gaattttacg
      481 aatggttcta gatttgccac gaataagtaa ggtttcagtg ctggcaatat tgcctctacg
      541 tttaatccct tttaacaatt cacctgaagt aatgccggtt gcaacaaaga cgatattgtc
      601 atcacgcacc aaatcttcta atttcaacac cgtattcacc ggtacattca tttcattaca
      661 gcgtaaaagc tctttttcgg tagcagttaa attttccggg ctattcccct tcacttggtt
      721 gcgaggaatt aaacgtgctt gcatatcgcc acccaatgca cgaaccgcag cccccgccac
      781 taccccttca ggtgctccgc cgataccata aagcagatcc aattcgctct ctggcaaaca
      841 gcattgcacc gcagccgcaa catcgccatc aggaatcgca atcactctta cccctaattt
      901 ttgcacatcg gcaattattt tttcgtggcg aggcttagca agatagcaat ggttaattgg
      961 gaaagtaatt tgcccttttt cgaggcgaca cggcgaagat tttgttcaag cggaagattt
     1021 aaatcaatca ttcctttgca ttcctgcccc accactaatt tttccatata catatcaggg
     1081 gcttttaaaa aggtttcttt gccgcctgct gctaataccg acaacgcatt tgcctgcccc
     1141 attgcagtca tacgagtacc gtcaatcggg tcaaccgcaa tactgatttc ctcatcttct
     1201 aggcgggata gaccgatttt ctcgccaata taaagcatcg gtgcttcgtc aatttcgccc
     1261 tcaccaatta ccacttcgcc acggatttcc atttggttca gcataaaccg cattgctttc
     1321 accgcagctt catcagcggc atttttatcc ccacggccta gccaagagta agctgctaga
     1381 gccgctgctt ccgtaacacg agaaaattca aaagaaagag ttcgtttcat ttttgttcct
     1441 tagtttcttg aaagaaaaac tttaaaagaa gagagattat attttatcag taacctgcaa
     1501 gctacgttac taaaaaatca gcgtttgaat aaaaattcac aaatagccaa actt
//
LOCUS       DANLUA010000274         1543 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLUA010000274 DANLUA010000000
VERSION     DANLUA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(1..181)
                     /locus_tag="PXB44_002551"
                     /pseudo
     CDS             complement(1..181)
                     /locus_tag="PXB44_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(342..635)
                     /locus_tag="PXB44_002552"
     CDS             complement(342..635)
                     /locus_tag="PXB44_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282975.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
     gene            complement(750..1349)
                     /locus_tag="PXB44_002553"
     CDS             complement(750..1349)
                     /locus_tag="PXB44_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282976.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
BASE COUNT          461 a          286 c          253 g          543 t
ORIGIN      
        1 tttcggttgc tgctacagca gattctgatc ttacatttaa actttgtgac tgttgtaatg
       61 catcaataag tttatttacc actgttccga gtgttttaat ttgctcggtt tgctcattaa
      121 tagtctgctt taactcttgc agttttaatg attgctcatc cacaaccact aatcgacggt
      181 tagctcaatt tgaggctctg tgctgccctc tactatggcg tgtaaatcca gcctaatacc
      241 ctctaaattt acaggctcgc catttgtttg cctaaaaaaa aggcgataac ttacgtcatc
      301 gcctctgaaa aacttaaatt tatgcttttg catttagacc cctacactac aatctgcccc
      361 gtctctttta agtgcttata gatacgacct aacataatct gcgttggcgt tttgccaata
      421 tcctcttttg ttagcggtgc atctgtaatc gctttggctg attgagcatc aatatatttg
      481 tactcacttg tgatgattac aaagtttgtt acctcgccat cggtatcatc acctgtgcca
      541 acaacatact tagcattgag tgagccgtca tcttgagttg agtaacttgc gatagttgag
      601 tacattgggt ttaagatttt gttaaaagtt gtcataattt ttccttttgt ggattgttga
      661 taaaaaaaga ccgcttgtat tgctacaagc ggcgatgaat gtgtaatcag aattttagtt
      721 cgcctctatt aaatgaaggc gtttaatgtt taatagcctg ttatatctac aaccagagca
      781 ttaaaataca ttgcttttga attataactc attcccgtct tatacaaacc agaataatgg
      841 tatttacaga acccaaattc aataaaatta ccacttactt tggcagatct agatgttgaa
      901 tatccgttat cgtagttaat gacaaaatca aagccaaaat tggataagat aatgccatat
      961 tgcctatttg cttgccctgt aaatctaaaa acattttttg atggatttag atcgacatat
     1021 tcaataggtc ttaaaaattt gtggtttgat gaaaatacaa catttcctgc tgagttataa
     1081 acctctatgc cgtaacctcc gcttttcggt acatttgtac taaaaatgag atattcagca
     1141 aatgttgcgt tttctgtagc taatgtggca ataccatttt ctcgatatag caaacatact
     1201 tgcccatcag aatatttaat gacaataatt tcatctgacg ctgcctgaat agttgcaatt
     1261 ttattagaaa agttagctcg tccttttctt tgtaaggata aacttctgaa attttcattg
     1321 atgttaaaag tagatagtcc gtaactcatt taatatgctc catatacaat ctgaatataa
     1381 tctgaatcat cagccggtag actaaaagga aaaatacaaa caacttctcc tgaaatcgtt
     1441 ttatcgttga tcgaaacatt ttctaaatga ataagattgg gaatattcgt tttataacct
     1501 gttgcttgtt ttcggtgaac tgaatgtaac atactaatga taa
//
LOCUS       DANLUA010000275         1502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLUA010000275 DANLUA010000000
VERSION     DANLUA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..335
                     /locus_tag="PXB44_002554"
     CDS             <1..335
                     /locus_tag="PXB44_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282977.1"
                     /translation="IDFGKQLANPVKFIKQNTAFLPKPIQQVVVFAVIFAGLPLFILG
                     FFILRIWAYKHISLYSEYKDMANSEISSKDTDLKSLFSDDEEQEDDEYQYGYDGPGIY
                     LGNYRVDE"
     gene            complement(381..>1502)
                     /locus_tag="PXB44_002555"
     CDS             complement(381..>1502)
                     /locus_tag="PXB44_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5282978.1"
                     /translation="QMNSELGDQQARVPIWWGLFHQINKGVTHALTASIPCKPDLRQV
                     RFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQNSQLSLSPAQVRETSWLGGRILIA
                     NSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANNGLKDRLLDDVK
                     QNFSAKVIDFFSSKENSDESILRTVLRAENMDISRGKVYPGYGGNLDRSFTNEVTKTA
                     SMFGLSLGSIGAFPAFDSMRQALPMVHSFALMALIILTPLIMVMSGYSLKTLVTLTFV
                     NFALVTLTFWWELARWLDSWLLSVLYDSGTHSRINPYFLENTQDDIIVNFVMGSMFLV
                     LPAIWLGSMSWVGVKMGEFASHLTTGSRDSKTAGSKIGK"
BASE COUNT          467 a          303 c          270 g          462 t
ORIGIN      
        1 ttatcgattt cggaaagcag ctcgctaacc cagttaaatt tatcaaacag aatactgcat
       61 ttttacctaa gcctattcaa caagtggtcg tttttgcggt aatttttgca ggattgcctc
      121 tatttatctt gggtttcttt attctcagaa tatgggctta taagcatatt tctttgtata
      181 gtgaatataa agatatggct aattcagaaa tatcgtctaa agatacagat ttaaaatcac
      241 ttttttctga tgatgaagaa caagaagatg atgaatatca atatgggtat gatggaccag
      301 gtatatatct aggtaattac cgtgttgatg agtaaaagta aatagagtgg tatataccac
      361 tctattattc agttgttaga ttatttccct atcttggaac ccgctgtttt agagtctcta
      421 gaaccagtag ttaaatgact tgcaaactct cccattttta ctcctaccca agacatactg
      481 cctagccaaa tcgctggtaa gaccaagaac atagaaccca tcacaaaatt cacaataata
      541 tcgtcttgtg tattttcaag gaaataaggg tttattctgc tatgtgtgcc tgaatcatag
      601 aggaccgaca gcaaccaaga gtcaagccat ctagctagtt cccaccaaaa tgtgagtgta
      661 actaatgcga aattcacaaa agttagcgta accaatgttt ttaacgaata accactcata
      721 accataatta aaggcgtgag aataatcaat gccattaatg caaatgaatg caccatcggt
      781 aatgcttgcc gcatcgaatc aaaagcaggg aatgctccga tactgcctaa tgacagacca
      841 aacattgaag ctgttttagt cacttcattt gtaaatgatc tgtctaaatt tcccccatac
      901 ccgggataaa ctttccctct cgaaatatcc atattctcag ctcgtaatac ggtacgaagt
      961 atggattcat cggaattttc tttactgctg aaaaaatcaa tcacttttgc agagaagttc
     1021 tgttttacat catctaataa acgatctttt aaaccattat tcgccgtatc ccaccactct
     1081 gcacaatttg gaaaaccacc ctctccatta ttcggcaatc cattatcccg agaagcatta
     1141 tagggaaata gactatgagg ttgttgggct cgataacggg gataaagctc gctatttgca
     1201 atcaaaatac ggccacctaa ccagctagtc tcacgaacct gtgctgggct caatgaaagt
     1261 tggctatttt gaattttctg acgtgccggt acatagcatt gttgtacaaa taagcgaacc
     1321 tctgtcaata atgctggatt attgattttc tcgtgttgaa cttcaaaacg aacttgacgt
     1381 aaatccggtt tgcagggaat tgaagctgtt aaggcgtgtg ttaccccttt gttgatttga
     1441 tgaaataatc cccaccagat agggactctc gcttgttgat cacccaattc agagttcatt
     1501 tg
//
LOCUS       DANLUA010000276         1478 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLUA010000276 DANLUA010000000
VERSION     DANLUA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            34..471
                     /locus_tag="PXB44_002556"
     CDS             34..471
                     /locus_tag="PXB44_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL5282979.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(756..1178)
                     /locus_tag="PXB44_002557"
                     /pseudo
     CDS             complement(756..1178)
                     /locus_tag="PXB44_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1308..1478)
                     /locus_tag="PXB44_002558"
                     /pseudo
     CDS             complement(1308..1478)
                     /locus_tag="PXB44_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019518307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          431 a          286 c          266 g          495 t
ORIGIN      
        1 aaaataaaag aaaaatttga aagagataat cctatgttaa gcttatttga tgcaggacag
       61 cgttacctca acactctgcc gaatcagaaa aaattagcca attttatgcc agattaccac
      121 attattcgct tggtaaaatt ttcctctaaa gtaatgcctg cttttgcttg ctttgcatta
      181 gtttggcaat attgctttac cgatccgacc caatctattt tagccaattc ggtacttact
      241 gctgttttcg ccatttctat tccgtttcaa gggttatatt ggcttggaaa acgggcaaga
      301 tcaccgttat ctttatcatt attggaatgg tatgagtcgc ttagacaaaa attaatttcg
      361 gcacaagtca aattagaaga aacacaagcg gtaccttctt tccaagattt tgcaaattta
      421 ctcaagctag cagaagagac ttggggagat gaatattttg atgagttata aaataaaaaa
      481 agccccactt aaaataagtg gggtaaaact tttgttctaa ttctggaggt ttgaattact
      541 gttttattgt ggggtactga tcttttttgc atagagagaa agaaaaaaat ttgggcggaa
      601 aaataacggc gagccccgtg aatacaaggg ttcgccgtta ttgtttggtt ggagctatgc
      661 aaaaaacaaa atgagcagga ttagaaaaaa acgagcagtt tttgcgttat tttttgcata
      721 ggtttgatcg gttttaaaac tattttaaat ctgttttaaa ctaaagaatg cccgatgtgt
      781 accacttgcc cgatgatttg taggttttgg gcttggtctg ctgtaatctt tattaactcg
      841 tactcttctc ggttgtctga aataagctct acaccgtccc atagcagttt tacacgctta
      901 acccaaacac tgtcgccgtt ttgtacaatg taaatattat tgtcgcctcg tatctcatta
      961 cgacttaaat caactagtag ctggtcgcca ttatcaatag tcggatacat cgagtctcca
     1021 cttgcccaaa aaacagcaca cttcttggca ttaacaccaa gcgtatgcaa caagctatca
     1081 gcataaggct cttgtccatc aggttttgtt acaccttcat taaagctccc aaagcctgca
     1141 gatactttaa tagatgcaaa actatcaatc attgagatta tgttattcaa aacggtgcct
     1201 gataattcag atcctttgtt ttgctcttca ggtgtaatct ctcttactat gctatccttg
     1261 attgcattta ttccactttt aaatccgagt gcttcttggg ctttacgagg caatgaagtt
     1321 atgtgatatt cattcccccc accttttact ccaactgcat cacgttttat ccagagttct
     1381 tttaatgcct ttcttgtaat gtttgttgct tgctttggaa ggtcaggcaa atccaattct
     1441 tctagttctt tggctgaaaa ccaaacttta tcaaatga
//
LOCUS       DANLUA010000277         1458 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLUA010000277 DANLUA010000000
VERSION     DANLUA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(204..1439)
                     /locus_tag="PXB44_002559"
     CDS             complement(204..1439)
                     /locus_tag="PXB44_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL5282980.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT          445 a          287 c          301 g          425 t
ORIGIN      
        1 ttggaaatgt tctttagtac tgcaatggat accctaattg cctcaagtaa atatagtaaa
       61 gactatttta tatttgctaa tttctttctg gctatcggtg ctacattatc ggctctttct
      121 tactcaatag ggattgagat tttgggttac gtactacttt tcttactttg catgttatgt
      181 attatttatc ggaaaatctt tatttaggat gttttctgga cagtatgaat cttccacaac
      241 attctggaat ctaaatttgc agaggcaaac atcgttaaga atacggctgc tccaaaatag
      301 aaagcggctt cccctaccga gaaactgaga atctgccctc gttcgagtag cgacatcatc
      361 aatgataaca cgaataaatc cagcattgac cagcgtccga caaaatggac gtagtgtagt
      421 agtttcattt gccaatgaat cgggtgtttc cacttgtaat gaattgccag caagagataa
      481 aaaataatgg taattttgct caccggcacc gcaatactag cgataaacac aatcgctgcc
      541 acggtgtagc tacccatttc cataaacaga agcacgccgg acattaatga atctgccgaa
      601 acctgacctg ccagttcagt tgtcgaaatc gggaaaatat ttgccggaat catcataata
      661 gtccctgcaa ttaagcaagc ccaaacccgt tgtattttta cgttgtcgga aatagataaa
      721 ttgctttcac aacgagggca gcgttgccgt ccttttaagt ctaaaatagt ttcatcaaag
      781 caatattcac aggtcgggca aagtgttgga ttgcttgggt gatcaaacgg caaggcgtga
      841 tattctgggt aaaaacgttc ccatgcttgt tttggctcgg ttttaataaa aagtagggtg
      901 tttaagagtg ttaccaacac aaaggcgatt aagttgatat cgaattgtag atcggcataa
      961 tctcgaattt taaaagcggc aaccgcaaga gcaactaaat agacatcaag cattacccat
     1021 tcttttattt tagttaagaa aatcagcgtg taacgggggc gttggcgaat aatacgttgt
     1081 agctgaatgg ttaaaattag caatacaaat gcaatcggca ttattaccgc gcagactagc
     1141 accataaaag cggtgtaagg gtagccctcc gttgccatct tccaaatacc gtcccatacg
     1201 gtagcattaa ttttgatgcc gagtaagtca atgctcatca agggaaaagt aagtgcgaag
     1261 ggaagcaaaa ttaaaatggc taatgccaaa aaagcactac ggtgcaagct ccaagtattg
     1321 cttttgcgta atatattatg gcagaccggg caaacggcat tgccatttga cgtgagcggg
     1381 actcgcactt gttgcccaca ttcgctacag ctttgtaaaa tgattctgtt tattttcatt
     1441 ttttgatctt ctattaaa
//
LOCUS       DANLUA010000278         1406 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLUA010000278 DANLUA010000000
VERSION     DANLUA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>1406)
                     /locus_tag="PXB44_002560"
     CDS             complement(<1..>1406)
                     /locus_tag="PXB44_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5282981.1"
                     /translation="HNKKLKELENKVSSLEQTNHVQQQDISRLNILLEQKNIEENRNL
                     ERMISYQLGYALINACKSFSGFISLPSELLKIRKLAKDKKQQKLLPSSPVANTPKQQT
                     ALVTSAPARSQSIDLTRGLTLLDPISELCWADAFTGYPLVRKSYADHIAGSTCKFAFF
                     ESAWQANKGSWIYAFTSPGLKHNNAQALLDAIQKLKDKSIPIIFWNKEDPMHYEMFKP
                     IAEKVDYVFTTDQLVVDKYKKELGHSNIWALPFAAPIKVTNPIGRFSLDTETVCFAGT
                     YYAQNHENRKKQMDMLLPALLAYNGCIYDRASKDTSGKYAYPEQYTHLIREGVNFNEI
                     VKLYKKFKVFLNVNTITQSTTMMSRRVYELLASGTPVVSTPSKAITAQFPGIVITVNN
                     EKEAELAVHKLLTDSYYWHKQSMLGIREVMSKHTYENRWEFACSVINNEPIKEKTPSV
                     RIIAVYHNYLDLAIYIDS"
BASE COUNT          430 a          251 c          230 g          495 t
ORIGIN      
        1 agaatctata tagatagcta aatctagata attatgataa accgcgataa ttcgaacact
       61 aggtgttttt tcctttattg gttcattatt gattacacta cacgcaaatt cccaacgatt
      121 ttcataagta tgttttgaca ttacttcacg tatgcccagc atggactgct tatgccaata
      181 atagctatct gtcagtaatt tatggacagc aagctcagct tctttctcat tatttacagt
      241 aataacaatg ccagggaatt gtgccgtaat agctttagat ggcgtagata caacaggtgt
      301 gccgctagct agaagttcat acacacgtct tgacatcatt gtggttgatt gagtgatggt
      361 attaacattt aaaaatactt taaatttctt atacaactta actatctcat tgaaatttac
      421 cccttctcgg ataagatgag tgtattgttc aggataagca tatttgccac ttgtatcttt
      481 agatgctcta tcataaatac agccgttgta agccaataat gcaggtaaca acatatccat
      541 ctgtttttta cgattttcat gattttgagc ataatatgtt ccagcaaaac aaactgtttc
      601 tgtatcaaga gaaaatctgc caattggatt ggttacttta attggggcag caaaaggcaa
      661 agcccaaata tttgaatgcc caagttcttt tttatattta tctacaacca attgatctgt
      721 tgtaaaaaca taatctactt tttctgcgat tggcttaaac atctcataat gcattggatc
      781 ttctttattc caaaaaatga taggaatgct tttatctttt agtttctgaa ttgcatctaa
      841 caatgcttga gcattattat gttttaagcc tggtgaagta aaagcataga tccatgaccc
      901 tttatttgcc tgccaagcac tttcaaaaaa agcaaattta cacgtgctgc cagcaatatg
      961 atcagcataa gattttctaa ctaaaggata ccctgtaaag gcatctgccc agcataattc
     1021 agaaatagga tccagtagtg tcaatcctct tgtcaagtca atagattgag atcgggcagg
     1081 tgcacttgta actaatgcag tctgttgctt tggtgtattt gcaacagggg aagatggcaa
     1141 taatttctgc tgtttcttat ccttagctaa cttcctgatt tttaataatt ctgaaggcaa
     1201 ggatataaaa ccactaaatg atttacaagc gtttattaaa gcatatccta actgataaga
     1261 aatcattctt tcaagatttc tattttcttc aatgttcttt tgctctaaca aaatatttaa
     1321 tctggatatg tcttgctgtt gaacatgatt agtttgctca aggctgctta ctttattttc
     1381 tagctccttg agctttttat tatgct
//
LOCUS       DANLUA010000279         1396 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLUA010000279 DANLUA010000000
VERSION     DANLUA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            527..1117
                     /locus_tag="PXB44_002561"
     CDS             527..1117
                     /locus_tag="PXB44_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282982.1"
                     /translation="MTTQITTAQINDLLKSHDIQGYANEWQGRRIYINLSAKNNESRG
                     DRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKDTAENTSPAESKETEQ
                     ENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIPVTATQEQITQAQAMV
                     DNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
BASE COUNT          531 a          277 c          244 g          344 t
ORIGIN      
        1 atttccgaaa ctatatacat ttttccgaaa ctcaaatttt aatcagatta taaaacaagc
       61 tattttcctt ttttctcatt ccaaaaatac catccaaaac atcttaccgc caaccaagtg
      121 ctccacgcaa aaataggtct attttgcgca atcaaacaat cccttaaccg cttatctgct
      181 tccgctctac tgacttttcc gtttacgccc acgatcgtgt tgatgacagc acgaattaat
      241 attttcaggg gcgtgtaccg caatcaattt taattataac caattgtttt taaattaaat
      301 tattgaatta catttaaaat acacacaaaa acacccacaa taaagcaata cctcagcact
      361 cgaagagcaa gatcgtcttt gagggatagg gggtaaaata aattttttaa aaatttgcaa
      421 aaaaatttaa aaaaactctt gcaataaaat tattatgcgc ctataattag gcgcataaag
      481 caaggctagg gagccagcta ataaccatca gccaaagggg gctaaaatga caactcaaat
      541 tacaaccgca caaatcaacg acttattaaa atctcacgat atccaaggct acgccaacga
      601 atggcaaggt cgccgcattt acattaacct ttcagcaaaa aataacgaaa gcagaggtga
      661 tcggaattat cagttgtaca ttgacttagc cacaggtgag ttagtgagca aacttggcaa
      721 aggattaaca acaagagagt tcgacgctgc cgtaaaagca tttgagaacg cttttaatgc
      781 taaagacacc gctgaaaata catcgccagc agaaagcaaa gaaacagagc aagagaacaa
      841 acaagaaaca actaagcttg taatcacaaa accaacatta aaaggctctg aaaaacaaat
      901 cgcttgggca gacgaaatca tcaatctttt tattgcgaag attggtgata cctctatccc
      961 agtaacagca acgcaagaac aaattactca agctcaagca atggtagata atttttttgc
     1021 aatgacaaac gctggaaagt ggatcgaaaa atttaaaggt ctaaatgata gtagcgaacc
     1081 aaagcaattt tggttatccg taaaatatgg atggtagatt gagataacaa ataagccgta
     1141 agagcaaagg cttacggcta aaacaaacag ataactggag attattatga ctacacaagt
     1201 aacaccacaa attatgcgca ttatcgggca aattgtcgct gctacttatg gtgacgatgt
     1261 gccaacaaac gtacagacaa taatcttgag ataccctata agagggatag ggtttatctc
     1321 tagtcgcaga gagctatcta ttaacaatgg agagatagca agattaatgg ataaaatacc
     1381 aggcgacctc gaggac
//
LOCUS       DANLUA010000280         1367 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLUA010000280 DANLUA010000000
VERSION     DANLUA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>1367
                     /locus_tag="PXB44_002562"
     CDS             <1..>1367
                     /locus_tag="PXB44_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282983.1"
                     /translation="EQGKKLYSRVGYDKGVWEALSVLLPERFDFEVFEVIDYSKEWSW
                     FEIIVENLFWRQPEKINMQKLHQFFKNSPYFNQATWINILYQLATEINHPLNIKYLHN
                     KLYSFSLADRDEIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIAVSW
                     LFTSTDKRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAVAYS
                     ENSSGLKALALEIDNQIFNQPETEEIYPNVLVRDYARHIIEYSLQKDEYSSDDIEIIR
                     ERITPPYRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYGDFGRYTFEA
                     KFRNWESYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRYWRRDDTNKI
                     ERIGKKYQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDNFYLPKTQIKIVPPT
                     KTDVF"
BASE COUNT          469 a          195 c          256 g          447 t
ORIGIN      
        1 tgaacaagga aaaaagctat atagtagagt tggctatgat aagggcgtat gggaagcatt
       61 aagtgtattg ttgccagaac gatttgattt tgaagtattt gaagtgattg attattcaaa
      121 ggaatggtct tggtttgaga ttattgtaga aaatctcttt tggaggcagc ctgaaaaaat
      181 caatatgcaa aaactacatc aatttttcaa aaatagtcca tattttaatc aagcaacttg
      241 gattaatatt ttatatcaac tggctaccga aattaaccac ccattaaata taaaatattt
      301 acataacaaa ctttattctt tttctttggc agatagagat gagatttgga cagcttttat
      361 tgctttggag tctgatcaat gcccagcaat tgaacgatta ctaacttggt gtcagaaatc
      421 aagtggtcaa gaaaatattg aacccgaagc actattattg ggtgcaattg ctgttagttg
      481 gctatttact tctaccgata aacgaagtag aaatggctat attaatgcac tggctcattt
      541 attatggaat agattggaaa ttgcattggc gttattcaag gaatttatag aggttaatga
      601 cccttatgtt atggaaggta ttttatctgc cattcacgga gctgttgctt attctgaaaa
      661 ttcatctggc ttaaaagcac ttgcattaga gatagataat caaatattta atcaacctga
      721 aacagaagaa atttatccca atgttttggt tcgtgattat gctcgccata ttattgaata
      781 ctctttgcaa aaagatgaat attcatcaga tgatattgag ataatcagag aaagaattac
      841 gccaccttat agaagttcat ttccccaaaa cttaccaacc aatgaagaaa ttgataaaaa
      901 atatcattct gatggacaaa gaatcattat ccgttcaatg acaactgaat atggtcgtgg
      961 tatgtgtgga tatggtgatt ttggcagata tacttttgaa gccaaattcc gaaattggga
     1021 gagttataaa ttcggcaatc tcatcaatgt tgatttatta agtaattatg cttgccaaat
     1081 gatttttgag caatttggct atgatgacaa gaagcacctt gagtttgata aagaaactcg
     1141 ttcaagatat tggcgaagag atgatactaa taaaattgag cgaattggca agaaatatca
     1201 atggttggct ttgtatgaaa tcttggcaag ggtgatggat aattttccta ctttttctaa
     1261 tcgctatgcc gatgaaatag ggcttgaata tattactaat ttggacaact tttatctacc
     1321 caaaacccaa attaaaattg taccgccaac taaaacagat gtatttt
//
LOCUS       DANLUA010000281         1347 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLUA010000281 DANLUA010000000
VERSION     DANLUA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            322..576
                     /locus_tag="PXB44_002563"
     CDS             322..576
                     /locus_tag="PXB44_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282984.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            776..952
                     /locus_tag="PXB44_002564"
     CDS             776..952
                     /locus_tag="PXB44_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282985.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            913..>1347
                     /locus_tag="PXB44_002565"
     CDS             913..>1347
                     /locus_tag="PXB44_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282986.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVIL"
BASE COUNT          494 a          237 c          231 g          385 t
ORIGIN      
        1 ccgctactca acatacaggg atggttatct tgataacgct cacgatactc ttgagcaata
       61 gctagtaatt gctgattagt catataaagt ctccaataac ataataaacc tgaaaacgtt
      121 cttagtattc tggcaacatt tttataaata gaaaccaaaa actatattca aatcattttt
      181 agtttctgct caacattcct atgtttttcc atacactgaa gtaaatttac ctaaccctat
      241 tttgctgtga aaccaaaact cctcacgctt atccttagta gcatttcgat gtcgctcact
      301 tatgccaccc ctcaattagg gatgatatgc cacgctgatc ctgaatatgt aaaatacaac
      361 tacgccggac tttgggaaaa aggcagcgat ggcacttttt tctgcaatta ccagcaattt
      421 atgcctgaag ttgaagctaa agaatcacta aggaaacaag aaccagaact tgatgataga
      481 tataacgaag aggaatatag cgaagaagaa tatcctgatg aaaatgatgc cagcgatgaa
      541 aacgataact acgatgaaac ttataatgtt ttctagtatg tatatggaaa ccggaggaat
      601 ccgtgattga aaaggcttgc atttccaaat atagtctata tattgtggta attgaaagta
      661 tttctatgct aacaagcata aggctatgta atcgaaaacc acccacaagg cctaaataca
      721 gtattattac agccatgagt aaccatggta atttaaatat ttaaggagtt ctataatggc
      781 aacatcatca tttgataaac gctttgtagt caaagatcca aaagctgtca aggtattaat
      841 tgaggcatta caaagcccaa tcaaagtagt tttacctaaa aaagacttag aaaaagaaga
      901 gaaagaagca ctatgtgcaa tcaaaaaacg cttagcctct atgggcttgt aaaagaacta
      961 ggtattgaaa aaacacaact ctttttggag caagcatttt cttgcagtag aaataaagat
     1021 gttgaaagtt ttctacttaa taaagcaatc agatttgaaa ccgcattagc agcagcaaca
     1081 catttaatca ttaatgaaca gggcgatatt ttggcttact ttaccctctc ttttaaagaa
     1141 attgaggtgg aagtaagcaa aagccaatta aaaaagctaa ctgctggttt aagtaatgat
     1201 tcacatttaa gagtattgct tatcggtcaa atcggcaaaa atgaaaaagt aattgacaat
     1261 ccagccaaat tgccagatat tctaaatcat atctatgcac aactttataa agcacaacga
     1321 gttttaggtg ggcgagtcgt tatatta
//
LOCUS       DANLUA010000282         1322 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLUA010000282 DANLUA010000000
VERSION     DANLUA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..88)
                     /gene="rrf"
                     /locus_tag="PXB44_002566"
     rRNA            complement(<1..88)
                     /gene="rrf"
                     /locus_tag="PXB44_002566"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(250..>1322)
                     /locus_tag="PXB44_002567"
     rRNA            complement(250..>1322)
                     /locus_tag="PXB44_002567"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          316 a          380 c          265 g          361 t
ORIGIN      
        1 gaatcaccac actaccatcg acgttactgc gttttacttc tgagttcggt atggggtcag
       61 gtaggaccac agcactcttg tcgtcgggaa aattcttttc tatttttgaa tttctttaag
      121 ttaacttcct aatctaactc ttagttcact ttaatagtta gattaacttc cttaacttcg
      181 attgatttct tctctagttt cgttcttctt ctcaatacaa aaacaagcct aaaaacattt
      241 gagcgttgta tagttaagcc tctcgggcaa ttagtactgg ttagctcaat gtctcacaac
      301 acttacacac ccagcctatc tacgtcgtag tctccaacaa cccttacaca cttatagtgt
      361 gggagaactc atctcgaggc aagtttcgtg cttagatgct ttcagcactt atctcttccg
      421 catgtagcta cccagcaatg cctctggcga gacaactgga acaccagtga tgcgtccact
      481 ccggtcctct cgtactagga gcagcccctc tcaattctcc aacgcccacg gcagataggg
      541 accgaactgt ctcacgacgt tctaaaccca gctcgcgtac cactttaaat ggcgaacagc
      601 catacccttg ggacctactt cagccccagg atgtgatgag ccgacatcga ggtgccaaac
      661 accgccgtcg atatgaactc ttgggcggta tcagcctgtt atccccggag taccttttat
      721 ccgttgagcg atggcccttc cattcagaac caccggatca ctatgacctg ctttcgcacc
      781 tgctcgactt gtctgtctcg cagttaagct tgcttatacc attgcactaa cctcacgatg
      841 tccgaccgtg attagcaaac cttcgtgctc ctccgttact ctttgggagg agaccgcccc
      901 agtcaaacta cccaccagac actgtccgag accgcgttcc gcaatcttcg ttagaacatc
      961 aaacgttaaa gggtggtatt tcaaggacgc ctccacaatc actggcgtga ctgcttcaaa
     1021 ggctcccacc tatcctacac atcaaaattc aatgttcagt gtcaagctat agtaaaggtt
     1081 cacggggtct ttccgtctag ccgcgggtac accgcatctt cacggcgatt tcaatttcac
     1141 tgagtctcgg gtggagacag cctggccatc attatgccat tcgtgcaggt cggaacttac
     1201 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
     1261 tcgatcagga gcttctcttg cgataacacc atcaattaac cttccagcac cgggcaggca
     1321 tc
//
LOCUS       DANLUA010000283         1318 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLUA010000283 DANLUA010000000
VERSION     DANLUA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            63..>1318
                     /locus_tag="PXB44_002568"
     CDS             63..>1318
                     /locus_tag="PXB44_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL5282987.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPV"
BASE COUNT          396 a          246 c          320 g          356 t
ORIGIN      
        1 gcgtaaaata acgtgaaatc aacttacgca aacgtttact taagcttatt aggagtccaa
       61 aaatgttaaa acgtgatatg aatatcgctg attacgatgc agtattatgg caagccattc
      121 aagatgaaga tcgccgtcaa gaagaacata ttgaattaat tgcatctgaa aactatgcaa
      181 gcccacgtgt aatgcaggct caaggctcac aattaaccaa taaatatgca gaaggttatc
      241 cgggtaaacg ttactatggg ggttgtgagt atgtggatat tgtagaacaa cttgcgattg
      301 accgtgcgaa agcattgttc ggggcagatt atgccaacgt tcaaccgcat tcaggctcac
      361 aagcgaatgc agccgtatat atggcgttgc ttaatccggg tgacactatt ttaggtatga
      421 gtttagcgca cggcgggcat ttaacccacg gtgcttcagt tagcttctct ggtaaaattt
      481 ataaagcaga acaatacggc attactgatg aaggcttaat tgattatgaa gcattacgca
      541 aacaagccca agaagtgaaa ccgaaaatga ttgttgctgg tttctctgct tattctcaag
      601 ttatggattg ggcaaaaatg cgtgagattg ccgatgaagt tggggcatat ttatttgtgg
      661 atatggctca cgttgcaggc ttaattgcgg cgggagttta tccaaatccg cttcctcacg
      721 ctcacgtcgt gacgacaaca acccataaaa ccttagccgg ccctcgaggt ggtttgattt
      781 tatctgcatc tggcgatgaa gaaatgtata agaaactgca atctgccgta ttccctgctg
      841 gtcaaggtgg accattgatg cacgttattg cggcaaaagc ggtttgtttt aaagaagctt
      901 tagagccgga atttaaggca tatcagcaac aagttgtaaa aaatgcaaaa gcaatggttg
      961 aggtatttaa gcaacgtggt tttgatgtgg tttcaaacgg tacggaaaac catttattcc
     1021 ttgttagctt tgtaaaacaa ggtttaaccg gcaaagcggc agatgctgca ttaggagctg
     1081 cgaacattac cgtgaataaa aactcggtgc cgaacgaccc tcaaaaacca ttcgtgactt
     1141 caggtattcg tgtgggtacg ccttctgtga cccgccgtgg ttttaaagaa gcagaggtga
     1201 cagcattagc cggctggatg tgcgatgtgc tagatagcat tggtaaggat aatcacgaac
     1261 aggtgattgc agagactaaa gcgaaagttt tagacatttg taaacgttta ccagtgta
//
LOCUS       DANLUA010000284         1265 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLUA010000284 DANLUA010000000
VERSION     DANLUA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            40..249
                     /locus_tag="PXB44_002569"
     CDS             40..249
                     /locus_tag="PXB44_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL5282988.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(298..1020)
                     /gene="bioD"
                     /locus_tag="PXB44_002570"
     CDS             complement(298..1020)
                     /gene="bioD"
                     /locus_tag="PXB44_002570"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL5282989.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
BASE COUNT          407 a          210 c          240 g          408 t
ORIGIN      
        1 tgccagaata caacctcatt ttgatatgag gaaaatagaa tgagtaagca aaagaatgat
       61 tacctccacc agcgaggtca aattaaagaa agtgcagtaa aagcattagt gactgatcca
      121 ctttttagac aacgtgtaga aagaaatcgt aaagggaaag ggagctatcg cagacaagaa
      181 aaacacaaaa aaactatcta tgggcaaaaa gaaaccccat ttaaaaatat tcggttagag
      241 tctttttaaa tggggtttta tttgtagctt attgtagttt tataagcctg ataaacacta
      301 tggtttactg aaataggcca acaacggttc tggattttga atataagaag aaagttcttt
      361 atgctctggg tgacccatat agggaatttg cccaagtaaa ggcgcattaa ttttttgtgt
      421 cagtagttca attaattcgt gataatgccg tagccctgga tttactctgt ttgcgaccca
      481 acttactaat tttacaccac gctgcaaaat ttcattcgca gtaagtagtg catggttaac
      541 acaaccttcc ttaattccca ccactaaaac aaccggcata ttgttttgca ttacccaatc
      601 agcaaaatag taatccttgt taataggagt tagccagcca taggttcctt ctacgacaac
      661 atttggatag gcattttcta gtcgttttaa atctgtgttt aatttttcca gttgaatatg
      721 atgaactgca tctaaagctg caaatacagg ggtactggaa tgaataaaag tatagctatt
      781 aatatcacga tagctaacat tagttggaca gctatctagt atggttaata cgtctgaatt
      841 atcttcagta gcataatcat attgagtagg ttctgtgggt aatgaatcat caccgccaca
      901 agcaatgggt ttatagccaa ttacaggaaa actatgtttt gtaagtgtct gtatgatggc
      961 tcgggtaacc acagttttcc ctacgttggt gtctgttccg gtaataaata atgagggcat
     1021 aaatggctcc tttcgaaaat atggctggta gtttaaccaa aaatagatca atttattctg
     1081 tctttgatca aatttccgtc ataaattcct tgttttattg ctgaacaggc aattaatgga
     1141 ctattccaat cgtattggct ggtaactaaa tcgattttta ggtttgtttg taacaaattt
     1201 ttatgcaatt tttctgaaat ttgcgtaaat aacggctctt ttattttgat caatggcgag
     1261 tttag
//
LOCUS       DANLUA010000285         1259 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLUA010000285 DANLUA010000000
VERSION     DANLUA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            10..1239
                     /locus_tag="PXB44_002571"
     CDS             10..1239
                     /locus_tag="PXB44_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL5282990.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
BASE COUNT          388 a          259 c          267 g          345 t
ORIGIN      
        1 gaaataatta tgcaattcag agaaattaat ccctcccctc gtttattaat gtgccctggc
       61 ccaacagatg ctgacccacg tgttttgcgt gcaatgtctg cacccatttt agggcaattt
      121 gaccctgagt ttacctcatt aatgaatgaa acaatggaaa tggaacgccg tttattccaa
      181 accaaaaatg agcaaactta tgtagtcaac tccacctctc gtggtggctt agaaactgtt
      241 ctaaccgcag cgatttgccc aggcgataaa gtgttaattc ctgcatttgg tcgttttggt
      301 tatttattaa atgaaatttt agctcgtagc ggtgcagata ttaccattat cgaaagagag
      361 tggggaaccg tatttgaacc agaagaaatt gaagcagagt tgaaaaaagg cggctataaa
      421 gcagttgcta ttattcacgg cgaaacctca acctctatga tgcaaccgtt agaagagatc
      481 ggcaaaatct gtaaacaata tgatgcaatg ttaattgtag ataccgttgc aaccttaggc
      541 ggtgtagata ttcgtgtgga tgaatggggc attgatgctt gtatcggcgg tacacaaaaa
      601 tgtatttccg ccccatcagg cacagcttta atcacttata acaaacggat tgaagagatt
      661 attgcaaaac gtaaacgtgt ggaaaaaggc attcgtgcag agtctgatgt agatggcaca
      721 ttaccgccaa ttccaagtaa ctacttagac ttggcacagc tacaagatta ctggagccca
      781 cgccgtttaa atcaccacac agaaaccact tctgcacaat atggtgtaca cgaagcgtta
      841 cgcattattt tacaagaagg cttagaagct cgttttgctc gccatatttt aaacgacaaa
      901 gcattatgtg caggtttaga agcaatgggt ttgaaaattt ttggcgatcg caaacataaa
      961 gcccccgttg taacggcatt ccacattcca gaaggtatga gtggcccagc attgcgtagt
     1021 gatattttaa accacttcgg tattgaatta gccacctcat tcggcccctt agatggaaaa
     1081 gtaatccgta tcggtaatat gggtttctca agccaaaaac gtaacgtact tttaacctta
     1141 ggtgcgttag aagccgtact tttaagccat aaagtaaaag tgccttcagg cgatgcggtt
     1201 gcagctgcct tggcagtcta taaagaggct gataaataac caccttccta aatttacaa
//
LOCUS       DANLUA010000286         1223 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLUA010000286 DANLUA010000000
VERSION     DANLUA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(65..>1223)
                     /gene="tet(H)"
                     /locus_tag="PXB44_002572"
     CDS             complement(65..>1223)
                     /gene="tet(H)"
                     /locus_tag="PXB44_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5282991.1"
                     /translation="AIGIGLIMPVLPTLLNEFVSENSLATHYGVLLALYATMQVIFAP
                     ILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRIIAGITGATGAVCASA
                     MSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMPFIFAAISHSILLILS
                     LLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATYFIIQLIGQIPATIWV
                     LFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEKTTIMISMSIDMMGCL
                     LLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGKLQGTLVSLTNITGII
                     GPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTTPKAVISTP"
BASE COUNT          405 a          252 c          252 g          314 t
ORIGIN      
        1 aataaagtcc tatcaacgtt attaagagcg gacaggactt ttatgatctt tataatttct
       61 agtattaagg ggttgaaata acagctttag gtgtggtttt tctttggtga aaataagcgg
      121 taataagcaa catagcataa agtattgccc ccatcagcca taacagacca tcccaataag
      181 cgacgctata actataaata aaggcaaata aaaggggacc aatgatcccg gtaatattgg
      241 ttaggctcac cagagtacct tgtaatttcc cttgcgcatt atcatcgaca gattttgata
      301 aataaccttg taatgcgggt tgccccatac ctcctgccgc taagcaaatt aatgctggta
      361 agatgaccca aacgtggcct atccacgcta ataataaaca gcccatcata tcaatagaca
      421 tactgatcat aatggtggtt ttttcgcccc atttttgtgc caatttccca gcgacaatcg
      481 cctgaaagaa aatatttaat acacccagaa ccgccaaaga cataccgata gaagttgtgt
      541 tccaatcaaa acgatattgt gtaaacagca cccagatggt ggcaggaatt tgcccgataa
      601 gctggataat aaaataggtt gctaaccaaa agtagaggct tttcttaaaa aaaacagtga
      661 ctgtatttga ggcagtttgg ttttcaggtg tcctattggc aacaagcgct tctctttttt
      721 gtgtttctcg gaaaaagagc aaagagagta ttaataatat cgagtgtgaa atagcggcaa
      781 aaataaatgg catatgagca ctgatatcac ctaataatcc ccctagcatt gggccgataa
      841 taaggccaac accaaaagca ccacctaaga aaccaaaata gcgagttcga tttttagcgg
      901 gagtcacatc actcatcgct gatgcacata cggcacctgt tgcgcctgtg atccccgcaa
      961 tgatgcgccc aatatagagc atccaaagtg tggttgagaa tgccattaaa agatagtcga
     1021 gtgccgcgcc taaaagggaa aacagcaaga tgggttttct gccgtattta tcagacagtc
     1081 gtcctagaat aggagcaaaa ataacctgca tggtagcata gagcgctaat agcacaccgt
     1141 aatgggttgc cagtgaattt tcactgacaa attcatttaa tagagtaggg agtactggca
     1201 tgataagccc gataccaatg gca
//
LOCUS       DANLUA010000287         1199 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLUA010000287 DANLUA010000000
VERSION     DANLUA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            10..168
                     /locus_tag="PXB44_002573"
     CDS             10..168
                     /locus_tag="PXB44_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282992.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            161..478
                     /locus_tag="PXB44_002574"
     CDS             161..478
                     /locus_tag="PXB44_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5282993.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            599..829
                     /locus_tag="PXB44_002575"
     CDS             599..829
                     /locus_tag="PXB44_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282994.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDNV"
     gene            928..>1199
                     /locus_tag="PXB44_002576"
     CDS             928..>1199
                     /locus_tag="PXB44_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5282995.1"
                     /translation="MFYSNNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTSYRYQQ
                     AVEQSGVDALLNQNRRVPNLKNRVDEATEQAVVKFAFDNPAFGQVRV"
BASE COUNT          426 a          209 c          236 g          328 t
ORIGIN      
        1 gagttatcca tgacagaaga ataccaaaac ttcttaatag agcaagtaaa acaaggactt
       61 gatgatatag aaaacggacg tgttatttca ttagaagaaa gccgtcaact gtgtcaaaaa
      121 accatatatg aaaccttgca agagctacag ggagccgtgt atgcttagtc gaaaagaggt
      181 gttaatgact cgcagagcaa gtatgaattt acaacaaatc attcgtaatg ttgtggaata
      241 tacttatagc tatgaaagtg gtgataaatt atctgcggaa attcataaca ctttggataa
      301 tctttcttat tttccacgaa ttggtgttat aggttcagta gaaaatacta gagaatgctt
      361 ttgccgaggt tatagaatta tatatcgaga aacagaaaat caagtgcaaa ttttagccat
      421 tatccattgt cgtcaacaat acccaaccga ctattatcgc tctgcattca aagcataaca
      481 ggagctataa aatggaaata aaaagaaaaa tgaccgtagc cgaattaaaa ctcaaggcaa
      541 tgctcgccaa gcctaccata gttcaataat attaggtgca aaaaacagga gaaccacaat
      601 gtctctactt aaaaaagcac aaacgtttat ctctattttt aatgaagtgc gagctaaaaa
      661 tgaagctcaa cgccagtctt caaacaattt aaatgttgcc ttgaaaaatg ctgctgaaat
      721 gagagctgag atgaatgggt tctcccttaa gcctcaaaag cgttctactt actttgctga
      781 ccattatggt tgtggtattc atgcagtaga aacctatgat aatgtgtaga agatcagact
      841 tgatctgaca attcactcta aaaagtgaga gtttcccgtt tagaatatga gtgtccaaat
      901 tcaatctaaa caaaaaagga aactctcatg ttttattcta acaaccctct cattaaacac
      961 aagaccggtt tattaaattt agcagaagaa ctgggcaata tctctcaagc ctgtaaggtg
     1021 atggggatga gtcgagatac atcctatcgc tatcaacaag cagttgagca aagtggggtg
     1081 gatgccttac ttaatcaaaa tcggcgtgtt cctaatctaa aaaatcgtgt ggatgaggca
     1141 acagagcaag ctgttgtgaa gtttgcattc gataatccgg catttgggca ggtacgagt
//
LOCUS       DANLUA010000288         1178 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLUA010000288 DANLUA010000000
VERSION     DANLUA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..>1178
                     /gene="nadR"
                     /locus_tag="PXB44_002577"
     CDS             24..>1178
                     /gene="nadR"
                     /locus_tag="PXB44_002577"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL5282996.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIK"
BASE COUNT          388 a          207 c          225 g          358 t
ORIGIN      
        1 atcaaaaaat cgagggaatc attatgacag attttgctta tcttcaacaa aagcgcaaac
       61 agcttaaact caaagtgaat gatatttgtg aacaagctgg ggtaacccga gcttatttta
      121 atcagttagt aagcggaaaa atcaaaaatc caagcgccaa taagcttaaa gccttgcatt
      181 ccgttttaga tatcgtagaa gatagcaacc agcgttccgg ggttattttt ggcaaattct
      241 atcctattca cacaggtcat attaatatga tctatgaagc atttagtaaa gtagatgttt
      301 tacatgttat tgtttgtacc gattctgaac gggatttacg tcttttccag gaaagtaaaa
      361 tgaaacgaat gccgacaaat gaagatcggc tacgctgggt acagcaaatt tttaaatacc
      421 aacaaaaaca gatctttatt caccatttag ttgaagacgg tattccgagc tatccaaatg
      481 gctgggaaga ttggtctggt cgtgttaaag cgctatttga agaaaaaagc attaatccta
      541 ctattgtttt tagtagtgaa attcaagata aagaatctta tgaaaaatat ttgaatctag
      601 aagttcaact tgtcgatcct actcgtgaat cctttaatat ttcagcaaca caaattcgta
      661 ataatccttt tcaatactgg cgctttattc cgaaagaagt tcgtcctttc tttgttaaaa
      721 ctattgcgat tttaggtggt gaaagcagtg gtaaatcagt gttggtgagc aaattagcga
      781 atgtatttaa cactacctct gcttgggaat atgggcgtga gtttgtgttt gagcaacttg
      841 gtggcaatga acaagcaatg caatattccg actatccgca aatggtgctt ggacatcaac
      901 gctacgttga ttatgccatg aaacatgctc ataaagtggc tattattgat accgattaca
      961 tcacaactca agcgttctgt attcagtatg aagggaaggc tcacccgttt ctggattcaa
     1021 tgatccgcga atatcctttt gatgtgacga ttttactttc taacaataca aaatgggtag
     1081 ccgatggttt acgcagttta ggatcgaaaa aacagcgtca acagtttcaa cacctactga
     1141 aaaaactact agaaaaacac catattcctt atattaaa
//
LOCUS       DANLUA010000289         1173 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLUA010000289 DANLUA010000000
VERSION     DANLUA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..236
                     /locus_tag="PXB44_002578"
     CDS             <1..236
                     /locus_tag="PXB44_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL5282997.1"
                     /translation="KTLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIYSYISP
                     KTGKRSTKNRIGSYPQMSLVEQSPQVFEQIARL"
     gene            288..875
                     /locus_tag="PXB44_002579"
     CDS             288..875
                     /locus_tag="PXB44_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL5282998.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            868..>1173
                     /locus_tag="PXB44_002580"
     CDS             868..>1173
                     /locus_tag="PXB44_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5282999.1"
                     /translation="MLEIKRQVTILRPTNYGKEENHYKHLYILLTDPCFDGETGKGDM
                     ILSVNCSSIKDGKALDETCILKAGTHEFITRDSFIFYRHIKIDSLDEIKANIEAGYFI
                     "
BASE COUNT          428 a          222 c          236 g          287 t
ORIGIN      
        1 aaaaaacact ttcagacaca ttactaaaaa atcttaaata ttccccaaat gcgaagccta
       61 tcgcagacag taacgggcta tttattcttg cacagaaaaa agcgaaagta tggatttata
      121 gctatatcag ccccaaaaca ggcaagcgaa gcacaaaaaa tagaatcggt agctatccgc
      181 aaatgagctt agtggaacaa tccccacagg tatttgaaca aatcgcacgt ctttaaaata
      241 ttaaattaag agaaagcact atttgcgatg tagcaggaga taaaacaatg tttgatgaat
      301 taaaaatagc ccaagcgacc gcctatttgc tacataaggc aggcggaaca atggagcata
      361 ttaaggtaat gaaattgctt tatcttgcgg atcgcttatc gtggcaaaga tacggctatt
      421 ctatttctaa tgatgaatat tattcattgc cttatggacc ggtattaagc aacacactca
      481 acctaatgcg aggagaagaa gcgagcttat accaaaaaac cagtatttgg ggcgagtgga
      541 tagcagataa ggaagatcac aaaatcagcc ttaaaaaatc cgtaaatacc gaagatgaat
      601 acttctttga ttgcctttca gagagcgaca ccgaaatttt agaagaggta tttgcgacct
      661 ttggaaatta taagccattc gccctagtgg aatataccca taaccctaaa tacattcccg
      721 aatgggaaga cccacaaggc agaagcaagc cgatttcttt aagcacgctt cttactaagc
      781 tagggaaaac gccaagcgag atcgaaagca ttttagagca acaggcggaa aaagggacaa
      841 tggataacct tttcaagggc ttaggcaatg cttgaaatca aaagacaagt aacgattctt
      901 agaccgacca actacggcaa ggaagagaac cactacaagc atttatatat tctgcttacc
      961 gatccgtgct ttgatggtga aaccggtaaa ggagatatga ttttaagtgt aaactgctca
     1021 agcattaaag acgggaaagc acttgatgaa acttgtattt taaaggcagg aacacacgaa
     1081 tttatcacac gggacagctt cattttctac cgccatataa aaattgattc gttagatgag
     1141 attaaggcaa atatagaagc cggttatttt att
//
LOCUS       DANLUA010000290         1156 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLUA010000290 DANLUA010000000
VERSION     DANLUA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..914
                     /locus_tag="PXB44_002581"
     CDS             24..914
                     /locus_tag="PXB44_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5283000.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
     gene            complement(964..>1156)
                     /locus_tag="PXB44_002582"
     CDS             complement(964..>1156)
                     /locus_tag="PXB44_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL5283001.1"
                     /translation="EGGLCKNIIIGILGSSLGRWVFGDVLGIGSAYSAGDFNIVGIFF
                     GVLGAALLIFILRKLSIIN"
BASE COUNT          406 a          232 c          196 g          322 t
ORIGIN      
        1 ttacaatata aagaagagag aaaatggata aactaaacgc aatcagttta ttttgtaaag
       61 taattgaaac ccaaagtttc actcaagcag cacaacaagc tcaaatttcg ttacccatgg
      121 caagtaaatt ggtggctcag ttagaagaac atctcaatgt gcgtttacta caacgaacca
      181 ctcgaaaaat tacgccaact gaggcagggt tgctctacta tcagcaatgc ttgcctattt
      241 taaatgaact aaaagaggcc gatgccagcg taagtaatat taactcaacg ctacaaggga
      301 aaataacaat gtctgtacca atggattttg gctctcgctt cattgcccct taccttggga
      361 aatttatgga agattaccct aatttagccc ttcatattga atttagtgac cgtcgggtag
      421 atgtgattgc agaaggcttt gatttggtgc tgcgtattgg tagccttgca gatagttcca
      481 ttgtagcgaa aaaaattgca gattcccatt tggttttagt tgcttctcca gattatttag
      541 ctcgatttgg cgaacctaaa ggcttagaag cattagtgga acgagactgc ctactctatg
      601 aataccatcc tcaatggcaa ttttctcaac aaggacaaaa aatgcaaatt aaaccacaag
      661 gtaaaattta cagtaacaat ggttatgcgt tagtacaaat ggcaaaagct ggtttgggca
      721 tcattaacat tccaaagttt ttaataaaac acgagctaaa aaacggagag ttgattccta
      781 ttatgccttt tttgacacag gaacctttac aaatcagctt actctatcca aaccgtcgtt
      841 atctctctcc taaagtgcga atcctaattg attttttcac acaactgatg caagagcaaa
      901 aagagtatat ttaacaaaaa agaagcagtt ataaaacaac tgcttccttt ctgaaatacg
      961 aaattagtta attatgctta attttctcaa aatgaaaatc aaaagagctg cccctaatac
     1021 accaaaaaag ataccgacta tattaaagtc tccggcacta taagcggaac caatacctaa
     1081 tacatcacca aatacccagc gtccaagtga cgaacccaaa ataccaataa taatattttt
     1141 gcaaagtccg ccttca
//
LOCUS       DANLUA010000291         1132 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLUA010000291 DANLUA010000000
VERSION     DANLUA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1051
                     /locus_tag="PXB44_002583"
     CDS             <1..1051
                     /locus_tag="PXB44_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283002.1"
                     /translation="ILTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQNNLQAQN
                     VPQAQNASQAQNAPQAQNAPQAQNAPQVENAPQAQNAPQVENAPQAEVTPPVPQPQSQ
                     KIDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKDFLVLNLS
                     DINAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRVNDNISDK
                     LTATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDGNAEIFTI
                     KGDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSDQKYLIGE
                     AKSDNWQAIMVSEKK"
BASE COUNT          420 a          209 c          212 g          291 t
ORIGIN      
        1 aattttaaca tttgctgcaa ccttagtttt aagtgcttgc ggaggaagcg gtagcggagg
       61 ttcgtcttca acaccgaatc accccaaacc agtactagta ccaaaaacac aaaataatct
      121 tcaagcacaa aatgttcctc aggcacaaaa tgcctctcag gcacaaaatg cccctcaggc
      181 acaaaatgct cctcaggcac aaaatgctcc tcaggtggaa aatgctcctc aggcacaaaa
      241 tgctcctcag gtagaaaatg ctcctcaagc agaggttact ccgcctgtac cacagccaca
      301 atcacaaaaa attgacggtt cttttgataa aattggttca gtaaaactca ataaagaggc
      361 tcaaactctt gagcttagta gattcacttt ggtggataaa ttaggcacac caccgaagtt
      421 tgataaagta agcggtaaaa aaattattga agaaaaagat tttctcgtat taaatttgtc
      481 tgatattaat gctgaacaac tctctggcga ttttcttatt cgccgtagcg atgatctatt
      541 ctatggctac tatcacgata caaatggcaa aaatcttgtc gatgctgccg ataaattcag
      601 tcaatatttt gtcgtgtatg atgagaaacg ggtaaatgat aatatctctg ataaattaac
      661 agcaacttac cgtaaaaaag aaggctttgt atatggttca aatccacata ctaaagaatt
      721 tgccgcacgg atcagcaaat tgggggatgt agaaattaaa tttgaaaatg gtcaagctca
      781 aggaagtata aaagacgaaa aagatggaaa tgctgagatc tttactatta aaggtgatac
      841 aaaacagtta gagattaccc caacggaaag taaccgaatc attatagcaa ttttagacca
      901 aaatcaaaaa agctatactc caggaatgga aaaagcaatt atggaaacta agtttattga
      961 ttcaaaggct ggtaattccg accaaaaata cttaatcggt gaagcaaaaa gcgataactg
     1021 gcaagcaata atggttagcg agaaaaaata aagttatctt ttgctaaaaa ctgaaataaa
     1081 aaggctgagt ccgggtaata tcggcctcag tcttttaaat tgtagaaaat ca
//
LOCUS       DANLUA010000292         1106 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLUA010000292 DANLUA010000000
VERSION     DANLUA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..259
                     /locus_tag="PXB44_002584"
     CDS             <1..259
                     /locus_tag="PXB44_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5283003.1"
                     /translation="TYRSYDALDVSEFHHERINHSELFAEKQNHINGIENFWNQAKRV
                     LRKYNGINRKNFPLFLKECEFRFNFGTPKEQLKTLRKWCEI"
     gene            265..603
                     /locus_tag="PXB44_002585"
     CDS             265..603
                     /locus_tag="PXB44_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283004.1"
                     /translation="MYYSPKDYLEEYNDLGTIRYKFQEENLYGFLRDGATLVANGIVN
                     EPSVDCFSQEIAQFTGCHIFSSLYIAFNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTP
                     TFKKTIVYAS"
     gene            593..>1106
                     /locus_tag="PXB44_002586"
     CDS             593..>1106
                     /locus_tag="PXB44_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL5283005.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAEQTAEYIKD
                     KENYHKFIENFYDQKRVEKPLNLETLGNYQYDSISENQKISFKAKNHYFGYENKIISN
                     GYGISLDEEFG"
BASE COUNT          379 a          162 c          210 g          355 t
ORIGIN      
        1 tacctatcgt agctatgatg cccttgatgt gagcgaattt catcacgagc gaatcaacca
       61 ttccgaactg tttgcggaga aacaaaacca tatcaatggc attgaaaatt tttggaatca
      121 agcgaagcga gtactccgaa aatataacgg aattaaccga aaaaatttcc ctttattctt
      181 gaaggaatgt gaatttcggt ttaactttgg tacaccaaaa gaacagctca aaacgttgcg
      241 aaaatggtgt gagatttagg gctaatctac tacagcccca aagattattt agaagaatat
      301 aatgatctag gtactattcg ttataaattt caagaagaaa atttatatgg ttttttaaga
      361 gatggggcaa cattagtcgc aaatggtatt gtaaatgaac caagtgttga ttgcttctct
      421 caagagatag ctcaatttac aggctgtcat attttttcaa gtttatatat tgcgtttaat
      481 actcaaagat cttttaaaag ccattgggat agtcgtgata tttttgcagt tcaaatgcag
      541 ggtaaaaagc gttggattat tcacactcca acatttaaaa aaaccattgt ttatgcatcg
      601 tagtaaagat atgcctgaat atgctcctaa tcttgatgat gtatatatgg atattgtatt
      661 agaagctgga gatgttcttt atttacctcg tggctggtgg cacgatccta ttcctgttgg
      721 agaagaaact gtgcatttag ctgtaggcat ttttcctgct tatactcata actatctgac
      781 ttgggtatct caaaatatgg ttgagaaaga aattgcccga gcttctttat cgcattatga
      841 gtcagataaa gagttgatag cacagttggc agaacaaaca gcagaatata ttaaagataa
      901 agagaattat cataaattta tagagaattt ttatgatcag aagcgggttg aaaagccact
      961 taatttggaa actttaggaa attatcaata tgattctatt tcggaaaatc aaaaaatttc
     1021 atttaaagcc aaaaatcact actttggtta tgaaaataag ataatttcaa atggatatgg
     1081 tatttctctt gatgaagagt ttggaa
//
LOCUS       DANLUA010000293         1074 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLUA010000293 DANLUA010000000
VERSION     DANLUA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            63..308
                     /locus_tag="PXB44_002587"
     CDS             63..308
                     /locus_tag="PXB44_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283006.1"
                     /translation="MTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKEFIE
                     PGATIRRSNDQPSIGSVDCDPRKKIHYKFDVHLMNTL"
     gene            527..>1074
                     /locus_tag="PXB44_002588"
     CDS             527..>1074
                     /locus_tag="PXB44_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HDL5283007.1"
                     /translation="MCKKAFPLWGLIFGLLLSTSAFSVSKNFNYQNILMESLHFSENI
                     ELSSLSKDWLLYSDRNTYHKYQNDEFEFQDKIEQAGKKLQKLVDDRKSEEAPIVFTIP
                     VNSEFGNYDFEQGKFEYQPLMNGHMYISSNQYTENLPRKINLYVSNPEFIDGIPMGKD
                     KAKEFLQKRKNSLGNINRSVIL"
BASE COUNT          368 a          172 c          206 g          328 t
ORIGIN      
        1 taggaaatga ttacgagtat tcgtatgaca aaactacttt caaaaacccg attaaaaagg
       61 cagtgaccaa cgacggttcg attatcgcca gaggctcagg ttttactact tatgccttag
      121 agttggaaaa tgatggcttg aaatatctag tcggatttgt tcaaccaaat ggaaatgcaa
      181 aagagtttat tgagccggga gcaacaattc gccgtagtaa tgatcaacca agtattggtt
      241 ctgtggattg tgaccctcgt aaaaaaatcc attataagtt tgatgttcat ttgatgaata
      301 ctttataata cctttgtggt gctagagcta gcccaagtta aaaaagacaa agctcggcag
      361 atgaacaatt gtggtgtcag agctgaccca cgttaaaaaa cacaatgctc ggtaggtaaa
      421 tcccttaggt ttaaaaaatc taagggattt ttgtttaaaa gacttgctct ttattatcag
      481 gtggatctat cataatttaa actctctaat gtgtcaggag ataaaaatgt gtaaaaaagc
      541 tttcccatta tggggcttaa tcttcggatt actgctatcc acttcagcat tctcagtgtc
      601 taaaaacttc aactatcaaa acatattgat ggaatcatta cattttagtg agaatatcga
      661 gcttagttcg ttatctaaag actggttact ttacagcgat agaaatacgt atcataaata
      721 tcaaaatgac gagtttgagt ttcaggataa aattgagcaa gctggtaaga aactacaaaa
      781 actggtggat gacagaaaat ctgaagaagc ccctattgtt ttcacaattc ctgtaaactc
      841 agaattcggg aattatgatt ttgaacaagg taagtttgag taccaacccc tgatgaatgg
      901 gcatatgtat atttcttcta atcaatatac tgaaaatttg ccaagaaaaa ttaatctgta
      961 tgtttctaat cccgagttta ttgacggtat acctatgggc aaggataaag ctaaagagtt
     1021 tttacagaag cgtaaaaatt ctttgggaaa cataaatcgc tctgttatat tgta
//
LOCUS       DANLUA010000294         1060 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLUA010000294 DANLUA010000000
VERSION     DANLUA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            75..>1060
                     /locus_tag="PXB44_002589"
     CDS             75..>1060
                     /locus_tag="PXB44_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDL5283008.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISLSDSKLADSSYL
                     FMLGEKYLKAQIDVANEKGVVYPPRYYQIDYQLKQLEPLFEKTKTVKTQTFS"
BASE COUNT          380 a          159 c          197 g          324 t
ORIGIN      
        1 agtaaatgaa tgcagtggaa ataagaaaat tatgtataat gctgtacaga aacatgcaaa
       61 ttttagagga aactatggca gattcagtaa aaagtcatga gcaaagctcg gatgagattg
      121 atttaattga gatttttcgt gctctatggg ataaaaaatg gtggattgtt ataacaacat
      181 tcattaccac attacttgct ggtatttatg catttactgc aaaagagcaa tggacgtcta
      241 aggcaacagt tattgcccct aaattaacag agttaggtga gtatttaaat gttcgcagag
      301 aatatagtcg gattttgcgt gtagatcctg ttgatccaaa tgcattgtct gcgagtttgt
      361 tcaacaattt taaccgatta gcacaatctc aagatactaa aaatgaattt tttatcactt
      421 ccaatgtgta taagacgtta gctgaaggaa aagatgagca ggggcaacgc acagttttat
      481 ccaatttgag taatgaaaat accagcttta ttattcctga tgcaaagaaa gatcctaatg
      541 caataggtcg tacactatct ttttctgcag agacacctat ggaggcgcag gacacccttg
      601 gtaagttggt tcaattcatt aatagcaaag catttgctgt tgaattagaa gattttctag
      661 tcgatttcca aaatgtgctt actgcattac aatatgaaaa atcattaatt caggaagatt
      721 tatctgtgaa taagtcggtt cagttggaaa acttaaataa agcctatgaa actgcacaaa
      781 aagcaggtat taaagaatat tcaaaagcat ttaatcaatc agatgcatca aatattgcag
      841 cagctttaag tgatactaaa atctcccttt ctgattcaaa attagcagat agttcatatt
      901 tatttatgtt aggggaaaaa tacctaaagg ctcaaattga tgtggctaat gagaaaggtg
      961 ttgtttatcc accacgttat tatcaaattg attaccaact taaacagcta gagccgttat
     1021 ttgaaaaaac aaagacagtt aaaacacaaa cttttagtta
//
LOCUS       DANLUA010000295         1044 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLUA010000295 DANLUA010000000
VERSION     DANLUA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            17..940
                     /locus_tag="PXB44_002590"
     CDS             17..940
                     /locus_tag="PXB44_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISApl1 family transposase"
                     /protein_id="HDL5283009.1"
                     /translation="MILLAQGKKQAEIAKALGRSSSTISRELKRHALESYSATNAQNS
                     YLKHRQNSKAQRKLEQPEYFNLVQEKFLTENWSPEQISARLKLEKSELSISYSTIYRG
                     IYSGLFDIGERKASRKLRHKGKTRHTKNHHEKRGKIQISNHLNDRPISAQNRSRFGHW
                     KADTVLGKAGGACLLTLTERKSRFELVKKIPAKKAEAVQKAMIELLDSHILRSITPDR
                     GKEFAQHRLVTEALGVEFYFPEPHQPWTRGTNENTNGLLREYFPKHQDINQWSEVDIQ
                     QVVNKLNLRPRKCLGWKTPYEVYFKKTLHLV"
BASE COUNT          352 a          215 c          217 g          260 t
ORIGIN      
        1 cgagcgagaa aagataatga ttttactcgc acagggcaaa aaacaagcag aaattgccaa
       61 agcactggga cgtagctcca gcaccatttc tcgcgagctg aaacgacacg ctctagaaag
      121 ctacagtgca acgaacgcac aaaacagcta tttgaagcat cgtcaaaata gcaaagcaca
      181 gcgcaaatta gagcagcctg aatatttcaa tttggtgcaa gaaaagtttc tgacagaaaa
      241 ctggtcgccc gaacaaatca gcgcacgatt aaaattggaa aaatctgaat tatccattag
      301 ttattcaacc atttatcgtg gtatttattc agggttgttt gatataggcg aacgcaaagc
      361 cagtcgcaaa ctgcgccaca aaggcaaaac acggcataca aaaaatcatc atgaaaaacg
      421 tggcaaaatt cagatatcca accatttgaa cgaccgtccc atttcggcgc aaaatcgcag
      481 tcgctttgga cattggaaag ccgataccgt actgggtaaa gcgggtggag cttgtttgct
      541 gacgctgacg gaacgcaaaa gtcgttttga gttggtgaag aaaattcctg ccaaaaaagc
      601 cgaagcagtc caaaaagcca tgattgaatt gctggattca catatattgc ggtcaattac
      661 gccagaccgt ggtaaagaat ttgcccaaca tcgtttggta acagaagcac tgggtgtaga
      721 attttacttc cccgagccgc atcaaccgtg gacacgggga acgaatgaaa atacaaatgg
      781 gttacttcgt gaatactttc cgaagcacca agacatcaat cagtggagcg aagttgatat
      841 tcaacaggtg gtcaataaac tgaatttacg accgcgtaaa tgtttgggtt ggaaaacacc
      901 ttatgaagtt tacttcaaaa aaacgttgca cttggtttga caattcaagc ttccaaatta
      961 aaatttttaa caacaaagtt tttcccatcg aaagttttgt ttatgttttc tagctttaat
     1021 tttaacattt agcttttcct tatc
//
LOCUS       DANLUA010000296         1039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLUA010000296 DANLUA010000000
VERSION     DANLUA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            197..445
                     /locus_tag="PXB44_002591"
     CDS             197..445
                     /locus_tag="PXB44_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283010.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            457..660
                     /locus_tag="PXB44_002592"
     CDS             457..660
                     /locus_tag="PXB44_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283011.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
     gene            699..>1039
                     /locus_tag="PXB44_002593"
     CDS             699..>1039
                     /locus_tag="PXB44_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5283012.1"
                     /translation="MKITHCKLKKSLQKKLLEFFVAEVTARTAADLLGIQPNTAALFY
                     HKIRLVIEHHLALEAHEIFEGKIEVDESYFGGHRKGKRGRGAAGKVAVFGLLKRQGKV
                     FTKYQIRNANAG"
BASE COUNT          341 a          137 c          251 g          310 t
ORIGIN      
        1 aagattgtaa aaaataaaaa tgtacaagat gttattgagg ctatagttct aatagtattt
       61 gttgtaatta taaacatcat agaggaatat ctcttatgaa agagttaaaa attgaggatt
      121 gccaatttgt ttcggcaggc ttgcacttgt tattgtagga gctgttgctg gagctgcata
      181 ttatgttggg aatggtatga tttctgggga aaaaatgact tgggagagtt tgggagcatc
      241 agctataggt ggggctattg gtggcggtat tggagcaaca atgaaagctg gtgcggtgac
      301 agcgtcaacc ttatctgctt taggtggtgg tatgggggag ggcttgtata aaagtacttc
      361 tcaaggtatc aaaaatagta ttggtataaa ggcgagttgc aatcaatcag gttcggatta
      421 tggtgatgga tgtgattatc aataagagag tatattatga aaattcaatt tccaatttct
      481 tatcaagaat ttcgtgaaaa ctattttgag aaaaaaccac ttttaatgaa aggagcaata
      541 tctcagaaag atttactttc ttggaagtca ataaatgaaa tattacctcg ctgtgattta
      601 atttctgagg atgcaattaa ggtaatgcat aaagggaaaa gggctcataa aaaagattag
      661 gggctgtagt agattagcat cactgctata ctacaaaaat gaagataacc cattgtaaat
      721 taaagaaatc tttgcagaaa aagcttcttg aattttttgt tgctgaagtg accgctagaa
      781 ccgctgctga tttattaggt attcaaccca atacggcagc cctgttttac cacaaaatta
      841 ggctggtcat tgaacaccat ttagcccttg aagctcatga aatttttgag ggaaaaattg
      901 aggtagatga aagctatttt ggtggtcatc gcaaaggaaa acgagggcga ggtgcggctg
      961 ggaaagtcgc tgtattcggt ttgttgaagc gtcaaggaaa ggtgtttaca aaataccaaa
     1021 tcagaaacgc taatgccgg
//
LOCUS       DANLUA010000297         1025 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLUA010000297 DANLUA010000000
VERSION     DANLUA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>1025
                     /locus_tag="PXB44_002594"
     CDS             <1..>1025
                     /locus_tag="PXB44_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5283013.1"
                     /translation="FLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQ
                     KADQHSVTLAIGGDVNKLVQRPVNSLPKQIVLALRYLLEQKIKLNTPKGPADGWFTED
                     GLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLGIVEESPSGGAIWSCRIQAD
                     SGWTPATTFTLLRVKPEVAWENINDRPAYFAGKVKVIEQITEEVNAEVQPPLVEETTK
                     ISLLPSKQSEAIIPSEETEISKAITSSDNATSAEVIINNENDLTSFVLDMFAPTETIE
                     QNISVISSNKEQQIVMTTLTEQEKVPPKKVKLEKSKKKNLQLTTLEPWNDMINPITGK
                     EFVNWLK"
BASE COUNT          337 a          191 c          229 g          268 t
ORIGIN      
        1 ttccttgcaa atcagctcat tcccaagtct gcgtttgatt ggctgggaca gtatccagaa
       61 gtcatttctt cactgatgta tgcaatgtct gggcataacg ataaagccgg gcttttatct
      121 gagatcgtgc agaaagccga tcagcatagt gttacgctag caatcggtgg cgatgtgaat
      181 aagctggtac agcgaccagt taattcattg ccgaagcaaa ttgtgctggc tctacgttat
      241 ttactggagc agaaaatcaa attaaatacg ccaaaaggac cagcagacgg ctggtttacc
      301 gaagatgggc tgtggctggt aagtaaaact gctgccgata atatccgaac ttatttactg
      361 ggacagggga tttccgtgcc taaagataat ccaaaactgt ttgatgaaat gcaatctctt
      421 ggtattgtgg aagagtctcc ttccggcggt gcaatttgga gctgccgtat ccaagccgat
      481 tccggttgga caccggcgac aacatttacg ctattacgag ttaaaccgga ggttgcttgg
      541 gagaatatca acgatagacc ggcgtatttt gccggaaaag tcaaagtgat tgaacagata
      601 actgaagagg tcaatgcaga ggttcagcct ccattagttg aagaaaccac taaaatttct
      661 ctattaccga gcaagcaatc ggaggctatt attccgtctg aggaaacgga aatatcgaag
      721 gcaattacaa gttcagacaa tgcaacatct gcggaagtaa tcataaataa tgaaaatgat
      781 ttaacttctt ttgtattgga tatgtttgct cctacagaga caattgagca aaatatttct
      841 gtcatttctt caaataaaga gcagcaaatc gtaatgacaa cacttacgga acaagaaaaa
      901 gttccgccga aaaaagttaa attagagaaa agtaagaaaa aaaatcttca attaaccact
      961 ctagaaccat ggaatgatat gattaatcca attactggaa aagaattcgt aaattggttg
     1021 aagga
//
LOCUS       DANLUA010000298         1004 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.304, whole genome shotgun sequence.
ACCESSION   DANLUA010000298 DANLUA010000000
VERSION     DANLUA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            207..998
                     /locus_tag="PXB44_002595"
     CDS             207..998
                     /locus_tag="PXB44_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5283014.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
BASE COUNT          291 a          221 c          206 g          286 t
ORIGIN      
        1 ttagccgaat tagccggggc aatcaaaccg cagcaaaata ttattgctca aactaccgct
       61 taatttatct taccaaatcc ctttttgaaa cctcgttcaa aaagggattt ctgatccgat
      121 gagccgctaa ctccatttgc ccgtttttcc tatatccgtt ataatcgaca caattttgcc
      181 ccaatcaata taaaagagga atttttatgg gtatcttaac tggtaaacgt attttagtga
      241 caggtttagc aagcaaccgt tcgatcgctt acggtattgc aaacgcaatg aagcaacaag
      301 gtgcagaact tgcattcacc tacttaaacg ataaattaaa accacgagtt gaagaatttg
      361 ccaaagaatt tggctcagac attgtccttc cgttagatgt agcaaccgat gaaagcatta
      421 ctgagtgctt tactgaatta agcaaacatt gggaaaaatt tgacggtttt gttcacgcta
      481 tcgcatttgc accgggcgat caattagacg gcgattatgt aaatgccgca acccgtgaag
      541 gttatcgtat tgcacacgac atcagtgcct atagctttgt tgcaatggct caagctgctc
      601 gccccttctt aaatgaaaat gccgcccttt taaccttaag ttacttaggt gcagagcgtg
      661 caatccccaa ctacaatgta atgtgcttag ccaaagcctc tttagaagct gcaacccgtg
      721 taatggcagc cgatttaggt aaagaaggta ttcgtgtaaa tgcgatttca gccggcccga
      781 tccgtacatt agctgcttca ggcattaaaa acttcaagaa aatgctttct gctttcgaga
      841 aaaccgctgc tttacgccgc accgtaacta ttgatgatgt aggtaactct gcagctttct
      901 tatgctcaga tttagcctct ggtgtaacag gtgaagtgtt acacgttgat gccggcttca
      961 gcgtaatggc aatgggtgaa ttaggcgacg aagagtaatt ccta
//
LOCUS       DANLUA010000299          989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLUA010000299 DANLUA010000000
VERSION     DANLUA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            21..584
                     /locus_tag="PXB44_002596"
     CDS             21..584
                     /locus_tag="PXB44_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283015.1"
                     /translation="MLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNNQTAAVNVM
                     TQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQGAANANVVV
                     NIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFGASNDEIKK
                     VLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            756..929
                     /locus_tag="PXB44_002597"
     CDS             756..929
                     /locus_tag="PXB44_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283016.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
BASE COUNT          342 a          190 c          198 g          259 t
ORIGIN      
        1 aacgctctaa aaaaacgtta gtgttagcca ctacttttgt tgccgcatta ctagccggct
       61 gccaaagcca atccaacaca ttaacattct ctaccccaac gccaactgct gtatttaaca
      121 caaacaatca aacggctgcg gtgaatgtaa tgacacaaga tttacgcacc tcgcgtgaaa
      181 tcgcaagcta cactaaaaac ggagaggtca ttcgtttaac cgcagtgcct gaagttgctg
      241 caatgttcca acaagccgtc caacaagacc tgaatgcaaa aggtttccaa attgtacaag
      301 gtgcagcgaa tgccaatgtg gtagtgaata ttcgtaaatt ctttgccaca gtagaacaag
      361 gaaacttacg ctacaaagcc gatgcacaag tgagtgtaaa agtggcagtg caaggttcta
      421 aaggaaactt tagcaaaaac ttcaacgcta cccgtggtta cgaaggtgca tttggtgcaa
      481 gcaatgatga aatcaagaaa gtgttagacc aagcctattc tgatgtggtg aaagctattt
      541 acaacgataa tgaaatcgcc aatgcaattc accagttcaa ataattaatt ttttctggca
      601 agcggtcatc tttcttcgat tttttgcaaa aaacgtggaa aaatgaccgc ttgttgtttt
      661 ctcgttttgt gatgtggctc gaaaaataag ataaattaac ttgattaaac gatcagtagc
      721 ccccacaata gagactcatc gcagggagga aaaatatgaa taatccttat cataatgtta
      781 gccaagaata tcttgattat cttgctaaaa aagtaaagca aggaaaggaa gatattaaaa
      841 acggtcggat ttattcaccc gaagaggtca gaaaaagagt tattgctgcc gcttttagag
      901 gtgctgaaaa tgccaaaaaa gttgcataat atttattata ctgaagaggc cttaatagat
      961 ttagagaata ttgccatctc agttagtga
//
LOCUS       DANLUA010000300          989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLUA010000300 DANLUA010000000
VERSION     DANLUA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>989)
                     /locus_tag="PXB44_002598"
     CDS             complement(<1..>989)
                     /locus_tag="PXB44_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5283017.1"
                     /translation="INEKMISSQLKEGDVLVNIVGATLEVVGRVAYVSSTFPKANITQ
                     AMSFVRLKSKYNKELLPTYLFAFLQSSYGKIQINRNARPTGQYNLNNEELGAIKVPLI
                     DLETQKQVDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQADSNPVNVKSLKESF
                     LQTGRLDAEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTKPMYQYIEIGDVNI
                     GDGSYQTNWIETQELPANAKTQAQTGDILISTVRPYRGAVTIIGENDQDLVVSGAFTV
                     LRRKENSVFNNEVLKVLLRSELYKDWLLQFNVGTSYPVIKDNDILNLPIPKISG"
BASE COUNT          307 a          176 c          140 g          366 t
ORIGIN      
        1 ccacttattt taggaattgg taaattcaaa atatcgttat ctttaattac aggataactc
       61 gtacctacat taaattgtag taaccaatct ttatatagtt ctgaccgtaa tagcaccttt
      121 aaaacttcat tattaaaaac agagttttcc tttctgcgta atactgtaaa cgcaccacta
      181 acaactaaat cttgatcatt ttcaccgata attgttactg caccacgata agggcgaacg
      241 gttgaaatta aaatatcacc tgtttgggct tgagttttag cattagcagg taattcttgt
      301 gtctctatcc aattcgtttg atatgaacca tcaccaatat taacatcacc aatttcaata
      361 tattgataca tcggttttgt ccaatcaggt ttttgggtga tatgtaaata ttcatcacga
      421 ataaaaacat aatcttgcga ttgaattaaa ttccagtatt gctcatattt agtttgataa
      481 tactctgcat ctaatcgtcc tgtttgtaag aaactctctt ttaaggattt aacatttaca
      541 ggattgctat cagcttgaaa agctctcaaa ccgagatttt ctaataataa agtttcagct
      601 tcttggtaac tttgtgttga tttttgtaca aaatcattac tttgcttaat gattttatct
      661 acctgctttt gtgtttctag atcaattaag gggactttaa tcgcaccaag ctcctcatta
      721 ttcagattgt attgtcctgt tgggcgtgca ttccggttaa tttggatttt tccataagaa
      781 ctttgtaaaa aagcaaataa atacgttggt aataattctt tattatattt tgattttaat
      841 cttacaaaag acattgcttg tgtaatattt gcttttggaa aagtgctact aacataagct
      901 acccgaccaa caacctccaa agttgcacca acgatattta ctaaaacatc cccttctttc
      961 agttgtgatg aaatcatttt ctcattgat
//
LOCUS       DANLUA010000301          979 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLUA010000301 DANLUA010000000
VERSION     DANLUA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            134..820
                     /locus_tag="PXB44_002599"
     CDS             134..820
                     /locus_tag="PXB44_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283018.1"
                     /translation="MAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGT
                     TISGGTISGTTITGGIIKGARIEGLTGKFTGELEINQLIGGNIYEAKTWTSKATGKSK
                     TYSETKNSFALSDTYYEYVCQFVLPATKATRTLYVVHTPTGESKSETFYGSGSNRTPT
                     TITLPSVIDISNTISPNVSNPFNDSGAILIANTQYVVTCRAWCAGRGRSAKVNFRAII
                     SSTNGSSYQL"
BASE COUNT          344 a          178 c          202 g          255 t
ORIGIN      
        1 cccttggcaa aatgccggtg taaccgctat ccacggtggc tcaatagtta ctaataccgt
       61 tactgctcaa caaattgcag caaataccat taccgccaac gaaatcgcag ccggcacgat
      121 tgcagctcga aatatggcag caaactcgat taatgcaagt catattgtgg ggagttcgat
      181 cactgcggat aagctaaatg taaacaattt agcggcaata tcggcaaatc ttggtaaggt
      241 aactgctggt acaattacag ggacgacaat tagtggtggg acaatttcag gtacaacaat
      301 tacaggggga ataattaaag gagcaagaat tgagggatta acaggtaaat ttactggaga
      361 acttgaaatc aaccaattga tcggaggtaa tatctatgag gctaaaactt ggacatcaaa
      421 agcaacaggg aaaagtaaaa catattcaga aacgaaaaac agttttgcat taagtgatac
      481 atattatgag tatgtatgtc aatttgtact acctgctaca aaagcgacga gaacattgta
      541 tgttgtccac actccaacag gagagagtaa aagcgaaact ttttatggta gtggtagtaa
      601 tcggacaccg acaacaatta cattaccgtc tgtaattgat attagcaata ctatttcacc
      661 taatgtatct aacccattta atgattctgg ggcaatactc attgctaata ctcaatatgt
      721 agtaacgtgt cgtgcgtggt gtgcagggcg tgggagaagt gctaaagtga attttcgagc
      781 aattatatca tctacaaatg gttcatctta tcaattatag gaggcaaata tgaaaaaaat
      841 tatactactt acaacaatta tggtttcagc ttgtacaact caatataagc aagactatac
      901 aacaaaacca accttttgtt atcaactagc ccctcaagag caattaccgg ggaaaaactg
      961 tattggctca ggagggcat
//
LOCUS       DANLUA010000302          975 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLUA010000302 DANLUA010000000
VERSION     DANLUA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..703
                     /gene="arcA"
                     /locus_tag="PXB44_002600"
     CDS             <1..703
                     /gene="arcA"
                     /locus_tag="PXB44_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL5283019.1"
                     /translation="IVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLSTNEINLV
                     LMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYITKPFNPRE
                     LTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEVNKLPRSE
                     FRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHPNTPEIIV
                     TIHGEGYRFCGNIEP"
     gene            723..>975
                     /locus_tag="PXB44_002601"
     CDS             723..>975
                     /locus_tag="PXB44_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL5283020.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFS"
BASE COUNT          339 a          164 c          202 g          270 t
ORIGIN      
        1 aattgttgag gatgagttag taacaaggaa tatgctgaaa agcatttttg aaggagaagg
       61 atatcaggta tttcaagcat ctaacggcga ggaaatgcac tctgttctga gtacaaatga
      121 aatcaatctt gtgctaatgg acattaactt accgggaaaa aatggattaa ttctcgcccg
      181 agaaattcga gagaatatga agctaccatt aatgttttta accggtcgcg acaatgaaat
      241 tgataagatc ctcggtttag aaataggtgc cgatgactat atcactaaac cgtttaatcc
      301 tcgagaattg actatccgag cgagaaattt attgcaacga acgatgatag ataaagaaaa
      361 cgacgttgta aaagagcaat ctgaaattga aagctatcgc tttaacggtt ggatcttaga
      421 cattaacagt catagtttaa tctcaccaga aggtgaggta aataaattac cacgtagtga
      481 atttagggct ttattacatt tttgccaaca tcctggaaaa attcaaacac gtgaagattt
      541 attgaaaaaa atgaccgggc gggaactaaa acctcatgat agaaccgttg atgttactat
      601 tcgccgtatt cgtaaacatt ttgaagacca ccccaatacc ccagaaatta ttgtcaccat
      661 tcacggtgaa ggctaccgtt tctgtggtaa catcgaacct taatcaagat aggagtaatt
      721 ttatgaaaaa attaactatt gtcgcattaa cttgcttatt ttctacccat ctattagccg
      781 ctcctgtcgg aaatacattt actggcatcg gtgtcggtgt agatgtaact acaacaaaat
      841 acaaagttga aggagtaaaa ggcaaggatt ccacaggtcc ggcattagtt gtcgattatg
      901 gtattgagca aggcaataac tttgtaggcg ttgtacaagg aaaagcaaaa gtaggtagta
      961 caaaagtatt tagcg
//
LOCUS       DANLUA010000303          967 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLUA010000303 DANLUA010000000
VERSION     DANLUA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            13..342
                     /locus_tag="PXB44_002602"
     CDS             13..342
                     /locus_tag="PXB44_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283021.1"
                     /translation="MKKALTLLALSLFALPVQADSLELARNKVKQIFISDEEPKVKDA
                     TWTAPFIFKVGVFDDGTKRDGYAEYVCQVLYDHGFKGKGVLVSVIDIKKLVQTGDWVD
                     LGKAKCQ"
     gene            442..702
                     /locus_tag="PXB44_002603"
     CDS             442..702
                     /locus_tag="PXB44_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDL5283022.1"
                     /translation="MKKLVLSILFVISPMALAYSCNDPIPYCKEMQSCEQAKFYLNQC
                     NAGRLDRDNDGIPCENVCGKKGKSEKKDDKKSDKSKSKKTSN"
     gene            734..>967
                     /locus_tag="PXB44_002604"
     CDS             734..>967
                     /locus_tag="PXB44_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL5283023.1"
                     /translation="MMNNQSVWHLFCDESGISGKPFYAFGALWIREDKISLFEKEIQK
                     LREKHYCTDEIKWQGTNSKRFSEFYRELVSFFFQ"
BASE COUNT          335 a          145 c          197 g          290 t
ORIGIN      
        1 ggggaaaccg atatgaaaaa agcacttaca ctacttgcac tatcactatt tgcattgcct
       61 gtacaagccg atagcctcga acttgctcgt aataaagtaa agcaaatttt tatcagtgat
      121 gaagagccaa aggtaaaaga tgcgacttgg actgcaccat ttatctttaa ggttggagtt
      181 tttgatgacg gcacgaaaag agatggctac gccgaatatg tttgccaagt tttatacgac
      241 cacggattta aaggtaaagg tgtgttggtt agcgtaatcg atataaaaaa actagtccaa
      301 acaggtgatt gggttgatct tggcaaagct aaatgtcagt aatacataag ggacagatta
      361 ttctgtctct ttgtatttaa tcattaatga agaaaccttt ttactacaca gttaatacta
      421 tattttataa tggggttcat aatgaaaaaa ttagtcctct cgattttatt tgttatttcg
      481 cctatggctt tggcttatag ctgcaatgac cctatccctt actgcaaaga aatgcaatct
      541 tgcgagcaag caaagtttta tttaaatcaa tgcaatgccg gtcgattaga tagagataac
      601 gatggcatac cttgtgaaaa tgtttgtggc aagaaaggaa agtccgagaa aaaagacgat
      661 aagaaatcag ataaatcaaa atctaaaaaa acttcaaatt agagacaggc gttgtgcctg
      721 attaaaaggg gtaatgatga ataaccagtc agtttggcac ttattttgcg atgaatccgg
      781 tataagcggt aagccttttt atgctttcgg tgcattatgg ataagagaag ataaaattag
      841 cttatttgaa aaagaaatac agaaattacg agaaaagcac tattgcactg atgaaataaa
      901 atggcaaggt acaaattcta aacgctttag tgaattttat cgtgaattgg ttagtttttt
      961 ctttcag
//
LOCUS       DANLUA010000304          961 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLUA010000304 DANLUA010000000
VERSION     DANLUA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            114..>961
                     /gene="msrA"
                     /locus_tag="PXB44_002605"
     CDS             114..>961
                     /gene="msrA"
                     /locus_tag="PXB44_002605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5283024.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          371 a          171 c          163 g          256 t
ORIGIN      
        1 atagagagta aaaccaattt tgatcttagc aaaaaagtaa gaaattatga atgtttaagg
       61 ttaaaccctc atccttaaaa aataaaatta cataagttat aaaggagaac tggatgaaat
      121 acaatcaact gttactatgt ttggtgtctg ctatctttac cacctcctca atagcaaaca
      181 tcacaacccc taacgagatg cgatctgaaa acttacagga tatttacttg gcaggaggat
      241 gtttctgggg cattgaagcc tatatggaaa aaatatatgg cgtaaaagag gctacatcgg
      301 gctatgccaa tggcaaaact gagcaaactg cttaccgaat tatcgggcaa accgatcatg
      361 cagaaacagt tcacgtgacc tatgatgcta ataaaatttc tttagaaaag ctgttgcaat
      421 attactttaa agtaattgat ccaaccagta aaaataaaca aggtaatgat attggccgcc
      481 aatatcggac aggtatttat tatcagaatg agcaagataa acaaattatt ttaaaagaaa
      541 tacaagctca acaagcaaaa tacaaacaaa aaatacaagt gcaagtagaa ccattaaaac
      601 attatatttt ggcagaagag tatcatcagg attacctaaa gaaaaatcca aatggctact
      661 gccatattga tttaaaccaa gccaacagca ttattatcga tccgaaagat tatcctaaac
      721 ctagtgatgc aacattaaaa atgaaattaa cgcctttgca atatagcgtt acccaacgta
      781 aaaatactga gcgttcattc agcaatgaat attgggataa taccgcaccg ggcatttatg
      841 ttgatataac tacaggagag cctctttttt catctctcga taaatatgac tccggctgtg
      901 gctggccaag ttttaccaaa ccaattatca atgaggttgt taactatacc gaagataata
      961 g
//
LOCUS       DANLUA010000305          957 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLUA010000305 DANLUA010000000
VERSION     DANLUA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>957
                     /locus_tag="PXB44_002606"
     CDS             <1..>957
                     /locus_tag="PXB44_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HDL5283025.1"
                     /translation="FSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKL
                     LEQLREQDSRFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFT
                     QFSAYLNLSEIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHT
                     VEKLVDDIEKNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQL
                     KRNIKRYFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLN
                     ENEYATSLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNEFHLE"
BASE COUNT          312 a          154 c          166 g          325 t
ORIGIN      
        1 tattttctga tatttatgct gagcggttta ttatttactt acgctatttt tataatcaac
       61 agcctgagtt attgttaccg aagttaaatt ttaattatca acagacgaat gaatatcagt
      121 ttgtaattaa gttactggaa cagcttcgtg aacaagacag tcgttttgag tttaatccgg
      181 aagttattcg atttctgacc tatattgcga tagccagtac agatctctac cgctttaaag
      241 attgtaattc ggataattac ccattgttgc tagataaggc ttgggaaatt catcgtttta
      301 tttttactca gttttctgca tatttaaatt tatcggaaat tcacgatgac acttgccgta
      361 aagatttgct taaaatgctg atccctatcg caatgaaaat gatgcttagc atttcagatg
      421 atgtggattt agggctattt aaccaaacag aactacatag aaatccaatt ttggctcata
      481 cagtagaaaa attagtggat gacattgaaa aaaactacgg gtatagccta tctattcgag
      541 aacagtattt tatttttgat attttttccg gaatggttaa tcgaattgaa ttagaacgta
      601 aaccgctccg actggcgatt attgccctta atggcagatt aagcacccaa cagttaaaac
      661 ggaatattaa gcggtacttt tccgactata ttttgaaaat tgaaaccaaa gtgctatatg
      721 agcttgccat aatggaagat aaaccttatg actactatct ctgtaatgaa tatggaaaaa
      781 atatggctat tccctataag cctatttatt ttgctgacag tagtttaaat gaaaatgaat
      841 atgcaacatc actcaacgaa gtattttatc acgcttatca atatgatgag gtgttgccta
      901 gcattaaatc gattgaaatt gaagcacaat atcgcctaaa tgagtttcat cttgagc
//
LOCUS       DANLUA010000306          936 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLUA010000306 DANLUA010000000
VERSION     DANLUA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(102..794)
                     /locus_tag="PXB44_002607"
     CDS             complement(102..794)
                     /locus_tag="PXB44_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5283026.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
BASE COUNT          283 a          177 c          173 g          303 t
ORIGIN      
        1 tgtttaataa cgtttatgtt acaaatttct cgcattttac ttataaattt atgaatggga
       61 agggtaaaat gacaatttgt ttaattcagt caaatttttg gctatttctc tcgatcatca
      121 acacctaaaa ttagataatt tccattaata tccgctaggc tacgaaagcc gatattataa
      181 atagcacatt ctctgctgac atttagattg ccataattaa aattatcatg gtagtggcca
      241 tggaaaattt tgctcgcccc taattttctt gctagctggt tgattacaga gaagccttga
      301 gggtgtggct ttggtgcttc gtgtgtaatt aaaatatcgg ctttttcatt ttccaatgct
      361 tcaaaatcag aagggaagat cgaagagcgg tgccttaatg gaataccacc acgccaaatt
      421 ttttccggag cagagtattg gcaaaaatgg atgggatcaa aaaacattgg acgattgggc
      481 ggcatccaaa tatgacctcg gaagacccct ccaatacctg caatttttat tccttgaatg
      541 tcaactactt taccgtgaat attgcgttct ttccagctac tattccaaat agcatcaaag
      601 gcagcaacgg ttttactgtc atggttacca tgaataaacc aaatatcaca atactgcgaa
      661 agccgatcta attcatcagg agtagtcaat tgaagatcgc ctagaataac taaagctacc
      721 tcctccgtct gctgttgtag aatcggataa agatgctcgt agctaccgtg tggatcgcct
      781 gcaaaaagta tcatgttatt tcctgtggca gttattcaaa ttcgtgaata ttatattgct
      841 taaactgtaa tgggattggt tcttcattta aaatagcttc taccacctct ttggcttgat
      901 tgtagcgatc caaataactt ggagattcaa ttttaa
//
LOCUS       DANLUA010000307          923 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLUA010000307 DANLUA010000000
VERSION     DANLUA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            42..668
                     /locus_tag="PXB44_002608"
     CDS             42..668
                     /locus_tag="PXB44_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5283027.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            717..>923
                     /locus_tag="PXB44_002609"
     CDS             717..>923
                     /locus_tag="PXB44_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5283028.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQA"
BASE COUNT          305 a          172 c          209 g          237 t
ORIGIN      
        1 ataaatcccc tttattttgg ctataattgt aatggtgagt catgactaaa attaaatttt
       61 acggcaattt aaaaaaattt ggtagtgaat ttagcttaga agttaaagat actgccgaag
      121 ctatccgagc cttatgtaca cagattagcg gattgcgtga ggcattacga gacggtgttt
      181 ataaagtccg tatcggcaaa cagtatttag acccgtcagc ccttgaaaaa gggctttttt
      241 attgtctgaa gaaagggcaa accattcatt ttacccctgt tatcaaagga gcgaaaagcg
      301 gtggcgtgtt taattttgtt ttaggtgcag ccttgatcgg agccgcattt tttactggcg
      361 gtgcttctat tgcggcttgg ggagctggag caaaaatgat gggtatgtta ggggcttcga
      421 tgttactagg aggagtttcc caaatgctca ccaaaatgcc aaaagccccc acaatgggta
      481 atgaaacaga aaaagaaagc tcaacggctt tttcaaattt aaataacttg gtgactcaag
      541 gcaaacctgt gccgttggct tatggcttaa ttcggaccgg ctcgcttgtg atatcgcagg
      601 gagttgagac gatcaccatt aaagaaaacc aaccggcaag caacaagaaa acaggcttta
      661 gaaaataagg gataattacc gattcagcac aagcacagca acttaatcag ttatctgtga
      721 acttgaccaa agcagagagc aatttaaatg ccaaaatcac ggaagaaaaa aaagctcgtg
      781 ttgatgcaga taaagtgaat gcagataaac tcacagacat aacaagccgt gttgcaaatg
      841 cggaatcaac tattaccaat ttccaatcaa ccaaagcgaa taaaagtgag gttgcgagcc
      901 ttgcacaaag ttcgttgcag gca
//
LOCUS       DANLUA010000308          918 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLUA010000308 DANLUA010000000
VERSION     DANLUA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..656
                     /locus_tag="PXB44_002610"
     CDS             <1..656
                     /locus_tag="PXB44_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5283029.1"
                     /translation="NQDVYIDGTILKQCQFINNRCISKSKQYSVRLARNNPFLNLTFK
                     SNKLSDNPAIISGKQGVIFCDDLSLGVNKHRLRLRFSSPINFTVNQEWQKMPFDRVLN
                     DINGHVDIVSGQFIARTGGTFHLKGKITVTNSSEDVLNLYQLRLLINNEEKERLINQS
                     VSPKNSKSNTNGMTTINFDTVVDLKVGDIVDLQYKTTHELTLLSDSFLSQIYVTQEF"
     gene            657..899
                     /locus_tag="PXB44_002611"
     CDS             657..899
                     /locus_tag="PXB44_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283030.1"
                     /translation="MTKKHYCTGWRNAPANINSCCHQHDRDYGIKGTVSRAEADKRLR
                     HCLISQGNPIKAWVFWVVVRLFGWIYYKEKNINGKS"
BASE COUNT          315 a          136 c          191 g          276 t
ORIGIN      
        1 ttaatcaaga tgtgtatatt gacgggacaa ttttaaaaca gtgccaattt attaataatc
       61 gttgtattag caagtctaag caatattctg tcagacttgc tcgcaataat ccgttcttaa
      121 atttaacatt taagagtaat aaattatcgg acaatcctgc gattatatca ggtaagcaag
      181 gtgttatttt ctgcgatgat ttgagcttag gagtaaataa gcacagatta agattgcgat
      241 tttcatcgcc gattaatttt actgttaatc aagaatggca aaaaatgccg tttgacagag
      301 tgttaaatga tatcaatggt catgttgata ttgtcagtgg tcaatttatt gcaaggacag
      361 gcgggacttt tcatttaaaa ggtaaaataa cggttacaaa ttcatcggaa gatgtgctga
      421 atttatatca actcaggctg ttaataaata acgaagagaa agaaagactg attaatcaaa
      481 gtgtttcacc taaaaacagc aaatcaaata caaatggtat gacaacaatc aattttgata
      541 ctgtagttga tttaaaagta ggtgacattg ttgatttgca gtataaaaca acgcatgagt
      601 tgacattact gtcagactca tttttatctc aaatttatgt gactcaggag ttttaagtga
      661 ctaaaaaaca ctactgcaca ggctggcgta atgcgccagc caatatcaat agttgctgtc
      721 atcagcatga ccgagactac ggtatcaagg gcacagtcag ccgagccgaa gccgacaagc
      781 ggttaagaca ttgcttaatt tcgcaaggga atccgattaa ggcgtgggtg ttttgggttg
      841 tggtgaggtt gtttgggtgg atttattaca aggagaaaaa tatcaatgga aagtcataaa
      901 acaggaaata tcattact
//
LOCUS       DANLUA010000309          911 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLUA010000309 DANLUA010000000
VERSION     DANLUA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            17..751
                     /locus_tag="PXB44_002612"
     CDS             17..751
                     /locus_tag="PXB44_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5283031.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FVLRVESEVLLEKAKILVEQEIENMR"
BASE COUNT          313 a          147 c          178 g          273 t
ORIGIN      
        1 taagccttat ggtttattgg cgagatcttc ttattttgca cgtttagcag aatggacttt
       61 attagaacat ttacttgtca aacagcctga aaatatttcg gtatgttcat tcggtcaatc
      121 attcggtaca agcggtcgtt tagatgcaga atattaccaa cctaaatttg caagcttatt
      181 tgcacaatta gccaatttca aaacgatgaa attaggtgag attgttaatt tacaaaaatc
      241 ggtagaaccg ggctcggaag cttatcaaac agagggaata ccctttgtgc gtgtatcgga
      301 tttatctaaa ttcggcatca gccaaacaga taaatactta catccgaaag attttggcaa
      361 tgttgtaaga cctaaaaaag acagtatatt actgactaaa gacggcacag tcggcattgc
      421 ttaccgtgtt ccgcaagatt tgaatgtgat tacatcaggg gcaattgtgc atctagagct
      481 taaaactgac gaagttttac ccgattattt ggcgttagca ttgaattctc ctgctgtaca
      541 attacaagcg gaaagagatg cgggaggctc tattatccaa cattggaaac cgtctgaaat
      601 tttagatgta gtgattccgg tattacctaa aaatatccag caaaccatta gcgacaaagt
      661 acaacaaagt tttgtattga gagtagaaag tgaagtttta ctggaaaaag ctaaaatact
      721 tgtagaacaa gaaatagaaa atatgaggta ataatgccta atcataatca agatttgaaa
      781 aaagagtggt ttaataaagc aaggatagat taccactctc cttttttaat gttatggctc
      841 agttgtaata gctggtataa cttccattat tccttaggta aggatagaac acatattgat
      901 agaataaaat c
//
LOCUS       DANLUA010000310          900 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLUA010000310 DANLUA010000000
VERSION     DANLUA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            125..643
                     /locus_tag="PXB44_002613"
     CDS             125..643
                     /locus_tag="PXB44_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL5283032.1"
                     /translation="MQKKLFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIA
                     EIEKTVELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNI
                     HRLAPNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLK
                     AIEDLEQELLFK"
     gene            complement(647..720)
                     /locus_tag="PXB44_002614"
     tRNA            complement(647..720)
                     /locus_tag="PXB44_002614"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Cys,seq:gca)
     gene            complement(736..811)
                     /locus_tag="PXB44_002615"
     tRNA            complement(736..811)
                     /locus_tag="PXB44_002615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Gly,seq:gcc)
BASE COUNT          319 a          168 c          158 g          255 t
ORIGIN      
        1 ttttcacgca cgaattgtaa gaaattagta aaaatcaacc gcttgtacgc aaagtgtttt
       61 gctttaaaat agaagttata tgaaataaaa ttcacttaaa ttttctttat tgataacatc
      121 aaccatgcaa aaaaaacttt ttatcgccca aataaaacaa aacctctcgg aattaaatgt
      181 attttcaacg gataatattt ttctgaattc accctatttc tcacagcaaa ccggtttggt
      241 gagtgtgttc attgctgaaa ttgaaaaaac agttgagcta ttattgaacc aaactgaggt
      301 gttatatagt gaattttacg ctgaaaaatt ggttaaacaa gttgatgcat taaagaatgc
      361 cgtagaaaaa atccaaagta agccagaatc agctcaattc cattcttcct atcaattttc
      421 tccaaatatt catcgtttag cgcccaacaa acgcttacaa gaataccgta aagcgttacg
      481 tgcattgaac gagaaaatta gctggttagt tgagcaaaat ttaaatacgc aaaatgaagc
      541 aactaaacaa accctgcaaa atcaaattac agagacagaa tatcgcaaaa tgaaatgttt
      601 aaaagcaatt gaagatttag aacaagagtt gctatttaaa tagtgatgga ggcgtgtccc
      661 ggagtcgaac cgagctacat ggatttgcaa tccagtgcat aaccgctttg ctaacacgcc
      721 ataagatttg gaaattggag cgggaaacga ggctcgaact cgcgaccccg accttggcaa
      781 ggtcgtgctc taccaactga gctattcccg catctgaatt cccacaacat tgcgctgtgg
      841 aacgaggtgc attttacgga tttatcattt tatgtcaatg ctaaatttaa cattctagat
//
LOCUS       DANLUA010000311          897 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLUA010000311 DANLUA010000000
VERSION     DANLUA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>897
                     /locus_tag="PXB44_002616"
     CDS             <1..>897
                     /locus_tag="PXB44_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283033.1"
                     /translation="GFKRCVNIPFFRFSKFFLNNAQRTAFSYVTHYIKKHSYAYSKAD
                     NIQELLVKFFSRKQPVMQSAFNHQKRIVKWLWAEFWDKNIISMTLRIDGYRNANLWHY
                     LRYQSRIEVLYKIANEHPNLLPLLAYVKPEYWKCSDLFSYQNWVRSRDKQPFILYAKC
                     QDKMLCESKGQWRWLKKQSRIVVDQCFLHVNQWQLLTEIQMPFKIPVCVICFILTNFS
                     MISCELDRSRQRRLLSIFIQYIYHYWKDNGFRQLKIHLSHLRVLFQDIEDYMIHEGYL
                     HGIPQTWQHLVYHLNLINSNTS"
BASE COUNT          305 a          133 c          154 g          305 t
ORIGIN      
        1 tggatttaag agatgtgtta atatcccttt ctttagattt agtaagtttt tccttaataa
       61 tgcacaacga acagcctttt catatgtaac tcattacatt aagaaacatt cctatgccta
      121 tagtaaggca gacaatattc aagaattatt agtaaagttt ttctctagaa agcaacctgt
      181 tatgcaaagt gcatttaatc atcaaaaaag gattgttaag tggttatggg ctgaattttg
      241 ggataagaat attatctcga tgacacttag aattgatggt tatagaaatg ctaacttatg
      301 gcattattta cgttatcaat cacggattga ggtattgtat aaaatagcaa atgaacaccc
      361 gaatctacta ccattattag cctatgttaa acctgagtac tggaaatgct cggacttatt
      421 tagttatcaa aattgggtaa gaagccgtga taaacagcct tttattttgt atgctaaatg
      481 ccaagataag atgttatgtg aatcaaaggg gcaatggaga tggttaaaaa aacagtcgag
      541 aatagttgta gatcaatgtt ttctacatgt taatcaatgg cagttattaa cagaaattca
      601 aatgcctttc aagatcccag tatgtgtcat atgtttcata cttactaatt ttagcatgat
      661 cagctgtgag ttagatcgat ctagacaacg tcgattactc tctattttta ttcaatatat
      721 ctatcattat tggaaagata atgggtttag acaattaaag atacatcttt ctcatctgag
      781 agtgcttttt caagatattg aagattacat gatacatgag gggtatttac atggtatacc
      841 tcagacttgg caacatttgg tttaccacct caaccttata aattcaaata catcaaa
//
LOCUS       DANLUA010000312          896 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLUA010000312 DANLUA010000000
VERSION     DANLUA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>896)
                     /locus_tag="PXB44_002617"
     CDS             complement(<1..>896)
                     /locus_tag="PXB44_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL5283034.1"
                     /translation="SDNKALHLGKIYHLIEQFELISRTDLAKLSGLAPASVTNLTKIL
                     IDNHFILERTVQTTLSRGRPSVGLAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIYKQ
                     DYTISAENYPRLNDFILNFITNFRQLYCVNTSQLLAVSISVIGKINAEKDSITQLGNK
                     NIFCNITSKLSPLFHCPILLSEHFQLWLLAESTVGSLINQDNAIFLQLDDTVNLSVLL
                     SGSLLSKQSKMNVDNMLMPRFSHLSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRYLP
                     NNLKSQEQKISWLCQKIDENQP"
BASE COUNT          305 a          142 c          158 g          291 t
ORIGIN      
        1 gcggttgatt ttcatcaatt ttttgacaaa gccagctgat tttctgttcc tgacttttta
       61 aattattagg caaataacga tcaatgagtc tcacaatagc tgggaacgtc acctgattga
      121 ctaatttata gcgatgaatt tcatctagtt cagggaatat ttcatcactg agatggctaa
      181 atctaggcat aagcatatta tcaacattca tctttgattg tttagacagt aaactaccac
      241 taagcaatac acttagattc acagtatcat ctaattgtag aaaaatagca ttatcttggt
      301 taattaatga gccaaccgta gattctgcaa gcaaccacag ctgaaaatgt tcacttaata
      361 aaataggaca atgaaacagc ggcgagagtt tagatgtaat gttacagaat atatttttgt
      421 ttcccaattg agtaatactg tctttttctg cattaatttt accgatcaca ctaatagata
      481 cagccaggag ctgactagta ttaacacaat atagttggcg gaaatttgta ataaagttaa
      541 gaataaaatc atttagccta ggataatttt ctgctgagat tgtataatct tgcttataaa
      601 tttctgcacc attgagttta caaagtgaaa tttcaatttt atgagatgaa acagtaacac
      661 aaagtagttg ccaaaaaaaa ttagaaacag ctaatccaac ggaggggcga cctctggata
      721 aagtagtctg aaccgtacgt tctaaaatga agtggttatc tattagtatt ttagtcagat
      781 ttgttactga agctggggct aaccccgata gtttagctaa atctgttctt gaaatgagtt
      841 caaattgctc aattaaatgg tatattttac ccaaatgaag ggctttgttg tcagaa
//
LOCUS       DANLUA010000313          883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLUA010000313 DANLUA010000000
VERSION     DANLUA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..229
                     /locus_tag="PXB44_002618"
     CDS             <1..229
                     /locus_tag="PXB44_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5283035.1"
                     /translation="NFGDHKSLAGTGGLYEMRIATGAGYRIYYAQQGNVIYILLHGGD
                     KSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            207..530
                     /locus_tag="PXB44_002619"
     CDS             207..530
                     /locus_tag="PXB44_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5283036.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          313 a          173 c          178 g          219 t
ORIGIN      
        1 caactttggc gatcataaat ctttagccgg tacaggcgga ctttatgaaa tgcggatagc
       61 aacaggagcc ggatatagaa tttattacgc acaacaagga aatgtaattt atatcttgtt
      121 acacggcggc gataaatcaa cacagcaagc cgatatagag aaagcgaaag cattatgggc
      181 tgaattaaaa actacagggg aaagcaatgg aaacaataca aatcaatgaa caggcagcag
      241 aaaagcacgg tttacgccct tttgacgtgg cggagttttt aaccgatgag gcaactattc
      301 aagcctattt agcggaagtg ttaaaagagg gcgaccaaga cgaattttta gaagccttga
      361 acaatgtggc aagggcaagg ggaatggcgg agcttgccaa gcaaacaggg ctagggagag
      421 agagcctata taaaacccta gccaaaggca gcaaaccacg ctttgacact attcaaaaaa
      481 ttctaggggc attcaattta gtgcttattc cacaagtcaa aaatggctaa aacttatcac
      541 ttgctacctg ccaaaacctc acattcaaat agccgttgat tgatattaac ggctttttta
      601 ttgcttaatt ctgtaccaat tacgcaccga ttgcggatca atacggggca agttttcgca
      661 aaaccttaat gaaatatgac cgcttgtgat tttgtacaaa acgacacaaa ccaatgtata
      721 aaatcaaaac ttgccacaaa tggcggaatg atattaaatg ccccacaaac tagcagtatt
      781 tcttaatgac ttttcatttt aagggtacat ttaagggtat gtttaacaag tcttaaatgc
      841 acttctcaat aataacaagc ctcatagcat acaattcaaa ctc
//
LOCUS       DANLUA010000314          883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLUA010000314 DANLUA010000000
VERSION     DANLUA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            85..870
                     /locus_tag="PXB44_002620"
     CDS             85..870
                     /locus_tag="PXB44_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL5283037.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
BASE COUNT          254 a          158 c          202 g          269 t
ORIGIN      
        1 tagaaataaa atagatagca ccacgaggtg ctttttttgt acctgaattt cccctcattc
       61 caattttaat cttgagaaac gtaaatgatg aaaaaaatta ccattttagg gcttggctgg
      121 ctcggactac ctttagctga agcattacaa aataagggct ggcgtgttgc aggtactaag
      181 cgaacggtcg gagaattagc gatagaatgt tatcctctag atttaaatgc gttagcagtg
      241 acaagcgaaa tcgagcaatt acttgctgct gatgcgttag tgattgcctt gccccttagc
      301 caaattcgcc ctgaaaatta cggttgcggt attcaaacat tagtttcgct tgcaatagaa
      361 aagggcttga aacatctggt tttcaccagc tcgacttcgg ttttgccaat gaaatcgggc
      421 gtttttgatg aaaatgcagt ggttgattct gctaacctat tagtaaaaat ggaaaattgg
      481 ttgctgagcc agccaattca ttgtgatatt ttgcgtttag ctggcttggt gggcaaaaaa
      541 cggcacccgg ttttttattt ggcgggaaaa cagaacttga gtggagcagg gcagccggtc
      601 aatttagtgc atttagagga ctgtattgca gccatttcat tgctgttgga gcagccgaac
      661 gggcagcgta tttttcattt atgtgccgaa cagcacccaa gccgtaaaga ttattatagt
      721 gaaatggcaa aacgttttgg gttagcggat ttgcattttt ctgaagaaaa tcaaccgctt
      781 gtaaggctga taaaagcaga caaaatttgc caagagcttg gttttcgtta ttactatcct
      841 gatccttatc aatttaaagt agatatttaa gtttaatcca tac
//
LOCUS       DANLUA010000315          867 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.321, whole genome shotgun sequence.
ACCESSION   DANLUA010000315 DANLUA010000000
VERSION     DANLUA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.321"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..320)
                     /locus_tag="PXB44_002621"
     CDS             complement(<1..320)
                     /locus_tag="PXB44_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5283038.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRT"
     gene            complement(317..>867)
                     /locus_tag="PXB44_002622"
     CDS             complement(317..>867)
                     /locus_tag="PXB44_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL5283039.1"
                     /translation="LLPPVSYDPNGRWLALSLEVEGRELDRIDARAKTLIDAVDVSTG
                     IFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQVAKELGYCIKISEP
                     QPFIAGLSRAGDMLWHKDIMWTFFVDICNPTDDYKRFRASVSMAGDALMGRYKDPILE
                     SLLEEVKPAFSRVWVRYLGDIE"
BASE COUNT          230 a          217 c          162 g          258 t
ORIGIN      
        1 atagtacgta actccgcaac cgatttgcag cgaccgatgt gtttgtaacc atcagggttg
       61 gctaatgcgt taaaattatc acgagtagca tcgcccgcaa ttttcttgat tgctttagcc
      121 acctgattaa cttgttcgtc atttgcttcg attcctgctt cagcaagtac actgtgcaat
      181 tcttgttggg tgttaatgac cgaatcttgg acgttattca aaaattcagc agtcacaatc
      241 gtgccatatt caccagtggc agggttgcca tctttaaaac gctgtgttgg ggtattaatc
      301 ggattcatca cttgtttcat tcaatatctc ctaaatatct tacccacaca cggctaaatg
      361 caggttttac ctcttcaagt agactttcta aaattgggtc tttgtatcgt cccattaacg
      421 catcgccagc cattgacaca cttgctctaa aacgcttgta atcatctgtg gggttgcaaa
      481 tatccacaaa aaatgtccac ataatgtctt tatgccataa catatcccca gctcggctta
      541 gcccagcaat aaagggttgt ggctcactaa ttttgatgca atatccaagc tctttggcca
      601 cttgagtgat atagcctatc gacaatccac ccaactgatt cagctttgca atcacacgct
      661 caattcgggt ttcaagcggt agtgagctat tcgccgttaa gccacaaacc cgctcccaat
      721 catcaataaa aatgcctgtt gatacatcaa cggcatcgat taaagtcttg gctctcgcat
      781 caatgcggtc tagctctctc ccctcaactt caagggataa cgctagccat ctgccgtttg
      841 ggtcatacga tacaggcggt aataaac
//
LOCUS       DANLUA010000316          865 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLUA010000316 DANLUA010000000
VERSION     DANLUA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..729)
                     /gene="dusB"
                     /locus_tag="PXB44_002623"
     CDS             complement(<1..729)
                     /gene="dusB"
                     /locus_tag="PXB44_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL5283040.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFET"
BASE COUNT          248 a          176 c          155 g          286 t
ORIGIN      
        1 agtttcaaaa aaatcattaa tttccttaaa tatccaagga tttccaaagc tagcgcgccc
       61 aatcatgatt gcatcggctt tggtatagtc gagaacaaac tttgcttttt ccgcagagtc
      121 aatgtctccg tttgctatca ctgggataga aattgcttct tttactgcct taatactttc
      181 atattcagct tcgccattaa ataaacagct tcgagtacgc ccgtgaatag tgagagcttt
      241 tatgccggct tgttctgcaa ttttcgcaat ttgcagacaa tttctatttt cctgatccca
      301 acctgttcta atttttagtg tcacagggac atcaacggca ttgactacag aatctaatat
      361 ttgagcgact aatttggggt tacgtaaaag agcagagcct gccatatttt tattcacctt
      421 ttttgccggg caacccatat taatatcaat aatatcggca ccatactcaa catttatctt
      481 tgcagccttt gccatttcaa caggatcaga acctgcaatt tgtacggcat ttaagcctat
      541 ttcatcgtgg tgtgctaacc gcaatcgtga tttctcagta tgccatacat caggattggt
      601 agacatcatc tctgaaaatg ttaaacctgc tccaagttgg ctacataagc ggcgaaaagg
      661 ctgatctgtg attccagcca taggtgcaag gaaaatacga tttttgattg catattgtcc
      721 tatttgcatt tttaaccttt tattaacaaa tttgaagtaa ttttaaccag agggatatcc
      781 aaataaaaag tacggtaaat accgtacttt cttataatag cttatggatc agtttagtac
      841 tgatttgagg gctggtatga tacgc
//
LOCUS       DANLUA010000317          858 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.323, whole genome shotgun sequence.
ACCESSION   DANLUA010000317 DANLUA010000000
VERSION     DANLUA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.323"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            23..733
                     /locus_tag="PXB44_002624"
     CDS             23..733
                     /locus_tag="PXB44_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283041.1"
                     /translation="MWKKIRIDFTDTVKCQFCSRMITSGKAIVVQNELGNISFSGPSC
                     AQNKNGQNVVNPKEKIVDITKGCLLHEPTTNDKTDKQVDVEQDNFTSIHDDEVHNYLD
                     NNAVNAYLTLRFEKLSHLTSIIKSQKLNEIYHNYELTGQISQKDESYIRVIMYGEAYP
                     LYTYQNLQAVYAAEYWLQSFIQHNPENDLNFIQDTLNQLRRKLTLTEKQIMGINNWFK
                     NSDGKMVKLKTNAFVRGK"
BASE COUNT          322 a          122 c          150 g          264 t
ORIGIN      
        1 tcactatatg gagagatatg ttatgtggaa aaaaattagg attgatttta ctgatacagt
       61 gaaatgtcaa ttttgcagta gaatgattac atcaggcaaa gctattgttg ttcaaaatga
      121 acttggcaat atctcttttt caggaccgtc ttgtgctcaa aataagaacg gtcagaatgt
      181 agtcaatcct aaagagaaaa tagttgatat cacaaaggga tgtttacttc atgagccaac
      241 aacaaatgat aaaacggata agcaagtaga tgttgagcaa gataatttca catccataca
      301 cgatgacgaa gtacataatt atttggacaa taatgctgtt aatgcatacc tgaccttaag
      361 atttgaaaaa ctatcgcatt taacctcgat tataaaatct caaaagttaa atgaaatcta
      421 tcataactat gaacttacag ggcaaatatc tcaaaaagat gaaagttaca taagagtgat
      481 tatgtatggt gaggcatatc ctttgtatac ataccaaaac ttacaagctg tttatgctgc
      541 tgaatattgg cttcaatcat ttattcagca taatccagag aatgacttaa attttataca
      601 agacacatta aatcagctca gaagaaagct aacattaact gaaaaacaga ttatgggcat
      661 aaataattgg tttaagaatt ctgatggaaa aatggtgaaa ttaaagacaa atgcttttgt
      721 aagagggaaa tagttaacat aattgtggtg gccacttatt ttgttacgta ggcccgacgt
      781 aagactggtt ttaaaagact ctttcctatt atttgcttgc ttattttgac aaattcaagt
      841 tatatgtaat tcaaaacg
//
LOCUS       DANLUA010000318          835 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.324, whole genome shotgun sequence.
ACCESSION   DANLUA010000318 DANLUA010000000
VERSION     DANLUA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.324"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>835)
                     /locus_tag="PXB44_002625"
     CDS             complement(<1..>835)
                     /locus_tag="PXB44_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283042.1"
                     /translation="VLTGQAGSGKSHLLGDFVQKRQNSGKYSLLLLGQDFTEKSNAWN
                     QILSKQLNLNITPEVFLSALDSIGEMQKERVILAIDALNEGEGKSLWNNQLNGLIELV
                     SQYPNIGLVLSVRDSYQDIIFNKLNIKNQDKLCYIKHTGFIDNEFEAIQIFFPYYGLQ
                     LPSIPLLNPEFSNPLYLKLFCESLKYQGETCVPKGHKGFNHIVKSYLDGIEKRICEQI
                     EQDIGSHLVQRAANVIAKLQLEEDKYSLDYMTVKRAICDELIVDIAETDAKKFLDYLI
                     KE"
BASE COUNT          276 a          152 c          106 g          301 t
ORIGIN      
        1 tttctttgat taaataatca agaaattttt tggcatctgt ttctgcaata tctactatta
       61 attcatcaca aattgctctc ttaaccgtca tataatccaa actatattta tcttcttcca
      121 attgtaattt ggcaataaca ttagctgctc tttgaaccag atgagaacca atatcctgtt
      181 caatttgttc acaaatgcgt ttttcaatac catctaaata agattttaca atgtgattaa
      241 atcccttatg accttttgga acacaggttt ccccttgata cttcaaagat tcacaaaata
      301 attttaaata taatgggttg gaaaattctg gattaagcaa aggaatgctt ggtaattgca
      361 agccataata aggaaaaaat atttgaatgg cttcaaattc attatctata aaccctgtgt
      421 gcttgatata acacaattta tcctgatttt taatatttaa tttattaaaa ataatatctt
      481 ggtagctatc tcgcacactc agaaccagtc caatatttgg atattgactt accaactcaa
      541 ttaatccatt aagttggtta ttccataatg atttcccttc accctcattt aaggcgtcta
      601 tggctaaaat aacccgttct ttttgcattt ctccgatact atctaatgcg gataaaaata
      661 cttcgggtgt gatattaaga tttaattgtt tggataagat ttgattccaa gcattggatt
      721 tttctgtaaa atcctgcccc aataacaaca aagaatactt tccagaattt tgtctttttt
      781 ggacaaaatc acccaataaa tgagattttc cactaccagc ttgaccagtt aaaac
//
LOCUS       DANLUA010000319          826 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.325, whole genome shotgun sequence.
ACCESSION   DANLUA010000319 DANLUA010000000
VERSION     DANLUA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.325"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>826
                     /locus_tag="PXB44_002626"
     CDS             <1..>826
                     /locus_tag="PXB44_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5283043.1"
                     /translation="NDNVIPFMEKVEEFTETLKTLSRDDLSAEERTTKTIDSVKLRIY
                     LNEQARNRKNYINGFVPTDWIPEITDQMTKSKNLWLKYEEEAKTTLKPYQEETISRVI
                     QDEINKKMAEIGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISKLDEA
                     KAALAKNQQTLKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAFGTDS
                     QATGNNAIALGANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGDNHS"
BASE COUNT          335 a          137 c          158 g          196 t
ORIGIN      
        1 aatgataatg taattccttt tatggagaag gtagaggaat ttactgaaac attaaaaact
       61 ttatctagag atgacttaag tgcagaggaa cgtacaacca aaacaattga ctccgttaaa
      121 ttaagaatat atctcaatga acaagcacga aatcgtaaaa attatattaa tggatttgta
      181 cctaccgatt ggattcctga aattacagat caaatgacaa aatccaaaaa tctatggtta
      241 aaatatgaag aggaagccaa aacaacatta aaaccatacc aagaagaaac aatatctagg
      301 gttatacaag atgaaattaa taaaaaaatg gctgaaatag gtactacaac actagagatt
      361 ggtggtaaag aacacgagct cgaggatctt gaaaaccaaa ttaatgcttt agctttaact
      421 gatgaagaaa aacttgcgga agatgtggaa aatgatttaa tctcaaagct tgatgaggct
      481 aaagcagcct tagcaaaaaa ccagcaaacg ctcaaagaaa agcgtgatga attactaggg
      541 ctaaataaca aattagatag ctcaccactt gttaaaaaag gtaaaaatgc acttgcagaa
      601 ggcacgaatg cctttgcttc tggtgaaaat gccatcgcat tcggcacgga ttcacaagct
      661 acaggtaata acgctatcgc actaggtgca aattcaaaag caaacgcaga aagtgcgatt
      721 gcaattggta aaggtgcaca agcattaaaa gaaaaagccc ttgctttagg cgaaaatgcc
      781 attgccaaca gagcatctgc tattgcgatt ggtgataacc attcat
//
LOCUS       DANLUA010000320          802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.326, whole genome shotgun sequence.
ACCESSION   DANLUA010000320 DANLUA010000000
VERSION     DANLUA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.326"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..647
                     /gene="tetR(H)"
                     /locus_tag="PXB44_002627"
     CDS             24..647
                     /gene="tetR(H)"
                     /locus_tag="PXB44_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL5283044.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(650..787)
                     /locus_tag="PXB44_002628"
     CDS             complement(650..787)
                     /locus_tag="PXB44_002628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283045.1"
                     /translation="MPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQGQLISQKGRQR
                     I"
BASE COUNT          252 a          147 c          169 g          234 t
ORIGIN      
        1 gctattttat tttgaggtag gtaatggcaa agctagataa agaacaagtt attgataatg
       61 cgttgatttt acttaatgaa gttggtattg aaggattaac aacgcgtaag ctggcgcaaa
      121 aaataggtgt ggaacaaccc acattgtatt ggcatgtaaa aaataaacgc gctttgttag
      181 atgcattagc agaaactatt ttgcaaaagc accatcatca tgttttgcca ttgccgaatg
      241 aaacatggca ggactttttg cgaaataacg cgaaaagctt ccgccaagcc ttattaatgt
      301 atcgtgatgg tggcaaaatt catgcgggaa cacgcccctc tgaaagtcaa tttgagacat
      361 cagaacagca actacagttt ttgtgtgatg ctgggtttag tctatctcaa gccgtgtatg
      421 cattaagctc tattgcgcat tttacattag gctccgtact ggaaactcaa gagcatcaag
      481 aaagccaaaa agagcgtgaa aaagtagaga cggatactgt tgcctatccg ccattattaa
      541 cccaagccgt tgcaattatg gatagtgata atggtgatgc tgcatttttg tttgtccttg
      601 atgtgatgat ctctggactt gaaacagtat taaagagcgc taaataaatc taaattcgct
      661 gacgcccttt ttggctaatt agttgccctt gtatgtcagc gataccaatg tgagcaatac
      721 aacctgcatt aaattgtaaa tagtcatctt cgatgccatc agagaaaatt acgccattct
      781 ctggcatcaa agatcctaat tg
//
LOCUS       DANLUA010000321          795 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.327, whole genome shotgun sequence.
ACCESSION   DANLUA010000321 DANLUA010000000
VERSION     DANLUA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.327"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..758
                     /locus_tag="PXB44_002629"
     CDS             <1..758
                     /locus_tag="PXB44_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5283046.1"
                     /translation="YLALNTKLDFTRYIQKARKFNEYNTDILYRSFDNFFAHYDTGVK
                     HNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGARAKYEMDNHQLSGFLRYRGVKH
                     KDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYVSRSSRSLIDEDLAINSRTVYAS
                     DVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDMTNTYKIKDKSNVYSVTTGLKKR
                     FANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
BASE COUNT          272 a          127 c          142 g          254 t
ORIGIN      
        1 gctatttggc tttaaatact aaattagact tcactcgcta catccaaaaa gcgagaaagt
       61 tcaatgaata taacactgat attttatatc gtagctttga taatttcttt gcccattacg
      121 acacaggtgt aaaacacaat aatctatcct taggcttgaa tacaaaatta agtgagaatt
      181 tacgtggtgg cttattcttt gaatacagta ataaaaatcg taatagcgta caaattggtg
      241 caagagctaa atatgaaatg gataatcacc aactttctgg cttcttacgc tatcgtggcg
      301 taaaacataa agatatgcta gcacgtaata acagtgttga tggctaccta aattatgctt
      361 atcgtattca attagatgat aaattaagca ttagcccatc agttggtgct tacgtttcac
      421 gttcttctcg ctcattaata gatgaagatc ttgcgattaa tagccgcacg gtctatgcaa
      481 gtgatgtagg attgaatatc cattataagc taaatgatat tgatgcttat attcgtccaa
      541 gtataggctt tgtcaatggt gatataactt tatctcaatc taatgatatg acaaacacat
      601 acaaaataaa ggataaaagt aacgtgtatt ctgtcacgac agggttgaaa aaacgctttg
      661 caaatggtgt ggcattaggg gctgatttca aattacatcg ttatggttct caaactactg
      721 aaagtaattt cggtctaaat gtaagttata actggtaata ccgtaaactg attacgattt
      781 aaaagttaga ctagc
//
LOCUS       DANLUA010000322          759 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.328, whole genome shotgun sequence.
ACCESSION   DANLUA010000322 DANLUA010000000
VERSION     DANLUA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.328"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            418..603
                     /locus_tag="PXB44_002630"
                     /pseudo
     CDS             418..603
                     /locus_tag="PXB44_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          255 a           92 c          149 g          263 t
ORIGIN      
        1 attattttta gaattatttt taatattggt ttttgtgatc agaattagat taaagggaga
       61 atgaaatgaa agaaccatca attttacaat taagcttagt agttggaggg aattctgaaa
      121 ataaggctaa tcaaccagat cttgtatctt atggcgttgc ttttgctaca gggtttgcaa
      181 gaggggggct ttaggcggtt tatccagtac ttctgttaat ttagcccttg atttagcgag
      241 gagcctacct attacaatac atgaaaattc tttacctagc ggtgtaaaag aattaattcc
      301 atcatcttta ccaataaagc ctacgtttgg agttccaggt agaacgaata ataaccaatc
      361 tggctcaaac tacgattgtg gtgatggtac agattatcaa ggctagtgga gatgaaaatg
      421 aaaattcaat ttccaatttc ttatcaagaa tttcgtgaaa gttattctga gaaacaacca
      481 cttttaatga aaggtgctat agagtagcaa gatttacttt cttggaaggc tatcaatgaa
      541 gtattacctc gttgtgattt actttctgaa gatgcaatta aagtaatgta taaagtaagt
      601 tgaaatactt taatggagaa agtatggtgg atttttctat tttgatatta gttatagtat
      661 tgcctgttat ttttgtgaaa gttatagatg tactgttgct tagtaagtta gatatgagta
      721 aggaaaaaaa tgacaaatgc caaataataa tatatttat
//
LOCUS       DANLUA010000323          743 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.329, whole genome shotgun sequence.
ACCESSION   DANLUA010000323 DANLUA010000000
VERSION     DANLUA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.329"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(93..725)
                     /locus_tag="PXB44_002631"
     CDS             complement(93..725)
                     /locus_tag="PXB44_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL5283047.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
BASE COUNT          216 a          166 c          131 g          230 t
ORIGIN      
        1 gatttttgtg atctaaatcg aaaatcagaa aatttctttc gttctaaaaa aataagctaa
       61 acagcctata tttcagcctc tcttacaaac acttaagcca ccgaatatcc catattttca
      121 ggggcaaatt cgccttggta gtggcgttca atcggaacgg cgattttact tgtaagatcc
      181 agcacttgat gcccttgtaa tttctctaca tcggtttgcc agttttccca acgcaaatcc
      241 tgataaaaat ggtttaaatc tccggctaat gcccagccta gaaattctga ataacctaat
      301 tgggtgtttt cccatttttt agcttttgga tgataataat aaactttgcc aagatctgag
      361 ccgattccgc cggcattaat ggcaaaatag ccaccgattg catcatccgc taccagcaag
      421 tggcttggtt gttcgccaga ttgtttaaaa gttttgccaa agttccaatc aaataagcct
      481 cgaggcaatt tttcgttgcc tgagcctaaa atgcgtaacc aaccgtggtc aataaatacg
      541 ccaccggttt catacaccat tgcccctagc ggtgagcgag tagaaagttg cataccaacc
      601 aatgcactgc cggcatcttc agggtaactc gctaaaattt cgtaatgatt tttggcttgg
      661 gtaaaccagt ctcttaaaat taaccaagcc gattgtttat ctaataattg ttctaaattt
      721 ttcatttgca aaatttcctt aga
//
LOCUS       DANLUA010000324          727 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.330, whole genome shotgun sequence.
ACCESSION   DANLUA010000324 DANLUA010000000
VERSION     DANLUA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.330"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(68..706)
                     /locus_tag="PXB44_002632"
     CDS             complement(68..706)
                     /locus_tag="PXB44_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5283048.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
BASE COUNT          228 a          149 c          110 g          240 t
ORIGIN      
        1 ataaattaca acgctcaaat tttaacaatt ttttgccaaa aaattaagat atttaaccgc
       61 ttgcacctta tattttaaaa ccttgtccat cgttccaaga aaccgtatat tctagaggta
      121 aacggcaact tggcaacgct gggtttgctg ctttaccgat agctagtagc ataacaggca
      181 aataacgttc tgcatctata ttcaataatt ttaatacagc agggcgctca aacacgccta
      241 tcgcatgagt atcatagccc ttatctttgg caattagcat taattgcatt gccgccaaac
      301 tagtatcaat taatacctga tctctaattt ctgcttgagt tgcttggtta tgaagattta
      361 gtaaaaaatc aaatttacgt tcacgaaact ctttatgcaa acacccagtt tctaccgatt
      421 gatcaacaat tgcgcctaat aatttttcat attgcaaatc tgccaatatc accacaatag
      481 cagacgctgt ttcacaaggt gggccattaa aggcaacatt ttttgataat gctgctttaa
      541 gaatttcaac atcaataatg acaaaacgcc aaggttgcag atttgctttt gagggagcaa
      601 gctgggataa agcgaccatt tcttctaatt cagttctact aatcttaatt gttgaattaa
      661 aagctttgat agttttacgc tgttcaatcg tatgtttcaa ttccatctct tttctcctat
      721 ctctcaa
//
LOCUS       DANLUA010000325          713 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.331, whole genome shotgun sequence.
ACCESSION   DANLUA010000325 DANLUA010000000
VERSION     DANLUA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.331"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            54..>713
                     /locus_tag="PXB44_002633"
     CDS             54..>713
                     /locus_tag="PXB44_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL5283049.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KS"
BASE COUNT          235 a          131 c          126 g          221 t
ORIGIN      
        1 aactttgttc taaatcaaat ttaggaatac ttttctaatt taaggttgaa tggatgaaaa
       61 ttgtatcaga acctaaaaca agcggacgtc atacttgcac aattcattgt caaaattgca
      121 gtattagcca actatgctta ccttttactc ttaatgaaac ggaattaact caactagata
      181 atattattga gcgtaaaaaa ccgattcaaa aatcgcaagt tatttttaag ttaggtgatg
      241 aactgcgttc gctttatgct atccgttctg gaacattaaa agcctatacc ttaagtgaaa
      301 gtggtgaaga gcaaattacc ggtttccatt tacctggcga tctaattggc tttgatgcaa
      361 ttaccaatat gcagcacgaa ggctatgctc aagcactcga aacctcaatg atttgtgaga
      421 ttccattcga tattttagat gatcttgccg gcaaaatgcc aaaaattcgc catcaaatta
      481 tgcgtttgat gagtaatgaa attaaaagcg atcaagaaat gattctattg ctctcaaaaa
      541 tgaatgcgga agaaaaacta gctgcatttt tgtataatct ttcccaacgt tactcagccc
      601 gtggtttctc tgcaaaagag ttccgtctaa ctatgacccg tggagatatc ggcaattatc
      661 ttggtttaac cattgaaact atcagccgtt tattagggcg tttccaaaaa agc
//
LOCUS       DANLUA010000326          713 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.332, whole genome shotgun sequence.
ACCESSION   DANLUA010000326 DANLUA010000000
VERSION     DANLUA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.332"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            141..>713
                     /locus_tag="PXB44_002634"
     CDS             141..>713
                     /locus_tag="PXB44_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5283050.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCI"
BASE COUNT          236 a          132 c          135 g          210 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcaccga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgatccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataatc
      661 cgaaaggtag aaagaattct aaatttattt ctgtaagtaa tgatttttgt att
//
LOCUS       DANLUA010000327          680 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.333, whole genome shotgun sequence.
ACCESSION   DANLUA010000327 DANLUA010000000
VERSION     DANLUA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.333"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            28..>680
                     /locus_tag="PXB44_002635"
     CDS             28..>680
                     /locus_tag="PXB44_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5283051.1"
                     /translation="MSNKSIPYQSFCWVIGTTSFRTAKLNLKIEAQLLLLDEFFNEVI
                     KESSWNWNNELQEKYYDFMKDRAFLTGEAKRKDKDAREKTSGLVDIGLITEDRLITDA
                     GRELLKVTSSGDFETNNVFNINRDSFIYLKQLLKTSIDVSGSIVRPFIAVVKCLTELE
                     FLSYDEFTYFVPLIRDDESAKQIISDIKLYREGKILPEEIIYKRLMQMENYKLAQEE"
BASE COUNT          261 a           75 c          140 g          204 t
ORIGIN      
        1 tatcctgtga attggaggaa taagtaaatg tctaataaaa gtattccata tcaaagtttt
       61 tgttgggtta taggaactac aagttttaga actgcaaaac taaatttaaa aatcgaggca
      121 cagttgttgc ttcttgatga gttttttaat gaggtcataa aggaatcaag ttggaactgg
      181 aacaatgaat tgcaagaaaa atattatgat tttatgaaag atagagcttt cctaacagga
      241 gaagcaaaaa gaaaagacaa agatgccaga gaaaaaacat caggattggt tgatattggt
      301 ttaattacag aagatagatt gattacggat gcaggaagag aactccttaa agtaacaagt
      361 agtggggatt ttgaaaccaa taatgtgttt aatattaata gagatagctt tatctatcta
      421 aaacagcttc ttaaaacgag tatagatgta agtggtagta tcgttagacc gtttattgcg
      481 gttgtaaagt gcctgacgga acttgagttt ttaagttatg acgagtttac ttattttgtt
      541 ccgctaatta gagatgacga aagtgcaaaa caaatcatat cggatattaa attatatcga
      601 gaaggtaaaa ttttaccgga agaaataatt tataagaggc ttatgcaaat ggaaaactac
      661 aaacttgcac aagaagaatt
//
LOCUS       DANLUA010000328          670 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.334, whole genome shotgun sequence.
ACCESSION   DANLUA010000328 DANLUA010000000
VERSION     DANLUA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.334"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>670
                     /locus_tag="PXB44_002636"
     CDS             <1..>670
                     /locus_tag="PXB44_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5283052.1"
                     /translation="LVNKICFICGKKDCPQMKKSKDYKDFLDALEKGDVDKADKIYNT
                     KFSQFSKSFGHEMEKNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNLSSVGLSNK
                     IRPLVNKDKAFGKSNGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADPLWALGTAT
                     ISGELKDIQIFPGGIARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYIIQDSWIKS
                     VRFS"
BASE COUNT          239 a           93 c          127 g          211 t
ORIGIN      
        1 tattagttaa taagatctgc tttatttgtg gtaaaaaaga ttgtccacaa atgaaaaaga
       61 gtaaagacta caaagacttt ttggatgctt tagaaaaagg ggatgttgat aaagcagata
      121 aaatatacaa tacgaagttt tcacagtttt ctaaatcttt tggtcatgaa atggaaaaga
      181 atttagagaa aaatcgtata cctcctattt atcagggagt tccatttgat tcttttgact
      241 tatatcagca ttattcttat ggtaatggag aggcattaaa tctttcatcc gttggattaa
      301 gtaataaaat tcgtcctcta gtgaataaag ataaagcatt tggtaaatcc aatggaagta
      361 tagaaagccg atttatttcg caatataaaa atgaaggacg cacatctttc tccaatgctt
      421 atgattttac aaaagaagca tcgggtattg cagatccgtt atgggcgtta ggaacagcta
      481 ccattagtgg agaattgaaa gatatacaaa tttttcccgg tggtattgct aggggaaata
      541 tccattatag tatcagggac agattcactg acccatttga tacattcaat tttactaaag
      601 gagaatggaa cccaaatgga acaccttata ttattcaaga tagctggata aaatcggtac
      661 gattttcgat
//
LOCUS       DANLUA010000329          658 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.335, whole genome shotgun sequence.
ACCESSION   DANLUA010000329 DANLUA010000000
VERSION     DANLUA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.335"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            86..>658
                     /locus_tag="PXB44_002637"
     CDS             86..>658
                     /locus_tag="PXB44_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283053.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKL"
BASE COUNT          227 a           89 c          147 g          195 t
ORIGIN      
        1 tatactttaa tataccaaat agcctatgac gtctcgtagt aaacagctag tttgtaagaa
       61 tagatatttg gaagggaaac gtaaaatgag agaaccatta ttagatgaag atgaattaaa
      121 aaaatggttg caaactattt ttactgacaa tttggtcatt attgtcggtt cgggtttgtc
      181 ctgtgccgaa ggtttgccgg gaatgggagc attagctgat agattaaaag aacgaatgcc
      241 tgaatgtctt gatgatatag ataaggtaac atggaatacc atttctgatt gtctggattc
      301 tgaaggcttg gaaggtgcat tgttgaagca tcaagcaaat gagacgatag aagccgctat
      361 tattaaaata acagccgagt atgtactctc cgaagagcaa aaagcgataa acaaatgtat
      421 tgctgagaat cagaagctca aattttctta tttgcttccc catatttcag cttcaaatcc
      481 taaaattgcc cgtgtaatta ctactaatta tgatagattg attgagtttg cggcagagta
      541 tgaaaattgg ggaatagatt caatgatggt cggtcgttat tggggaaaac ataatcctga
      601 tttgagtagg aaattgcaga taagagacat tagagtaaaa ggtaaatgtc caaaattg
//
LOCUS       DANLUA010000330          656 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.336, whole genome shotgun sequence.
ACCESSION   DANLUA010000330 DANLUA010000000
VERSION     DANLUA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.336"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>656)
                     /locus_tag="PXB44_002638"
     CDS             complement(<1..>656)
                     /locus_tag="PXB44_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5283054.1"
                     /translation="IKLDMIPKYYFKEIIDVLYTETFSRDNNLRADAPLETISEAFKL
                     DISKVYVALSKDLGITIKSPEQAATYVNDERYRRFNTLIDKKFNDSVLIELLNCFEKR
                     DDKRIEELVTDEAAIPTIFEYILGIIWYKVSERQGNILDFMKLSLEANLLPKTHAAGG
                     YADIIYEYEACTSYPKHSLLLEATLADGNNQRRMEMEPVSRHLGDYRIRFNNPFDYSL
                     "
BASE COUNT          204 a          129 c           86 g          237 t
ORIGIN      
        1 taggctgtaa tcaaacggat tattaaatct aatcctataa tcgcctaaat gtcttgaaac
       61 aggctccatt tccattcttc tttgattatt tccatctgca agggttgctt caagcaataa
      121 tgaatgtttc ggataagagg tgcacgcttc atattcatat ataatgtctg cgtaacctcc
      181 agctgcgtgg gtcttcggca ataaattggc ttctaatgat aacttcatga aatccaaaat
      241 atttccttgt ctttcgctca ctttgtacca tataattccc aaaatatatt caaaaatagt
      301 aggtattgca gcctcatctg taacaagttc ttcaatcctt ttatcatcac gtttctcaaa
      361 acaatttagt aactctataa gcacagaatc gttaaatttc ttatcgatca aagtattaaa
      421 tcttctgtat cgttcatcat ttacataagt tgcagcttgt tccggactct taattgtaat
      481 tcctaaatct ttacttaaag ctacatatac tttgctaata tctaacttga atgcttctga
      541 tatagtttca agtggggcat cagccctgag attattatct ctactaaaag tttctgtata
      601 cagaacatca ataatttctt taaaataata ctttggaatc atatccaact taatca
//
LOCUS       DANLUA010000331          654 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.337, whole genome shotgun sequence.
ACCESSION   DANLUA010000331 DANLUA010000000
VERSION     DANLUA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.337"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          203 a          120 c          110 g          221 t
ORIGIN      
        1 taatcgagaa ttatgttctt ttttattttg ttattctctt taggtataaa aaaacccggt
       61 aaataccggg cttttatttc aattggttat ccaacttgcg agagcaatta ccgcaataaa
      121 aataactaac caaataagtt gtaattttac gcttttgcgg ttgatctcct ggttgtgtaa
      181 tcggcgctgt tctaactttt gccgatatat cgctaaatcg gcttctttaa acgagaagcc
      241 acaatgtatg caatgttcta aactgtcgct aatacgcttt cgacactccg gacaacggtg
      301 taatgccata attacctcaa actattactt tgcataatcg gctatccgta cattgttcaa
      361 tatgaacatg gtagtattga tgagtatcac gattgaaaaa caagaaagat tgattacctc
      421 ttatttttgt tcccataatg tagccgttat cactgttggt ggataatatg tagtattttt
      481 gattctctat ttcgatgtaa tcataggtag ttcttagtcc ggcacgcaaa taaccaatat
      541 gcaatgagag caaactgaaa tagagaaaaa taaagatgtt aaacagggca aaacttcctc
      601 ctaatagcat ctttctaaag tccagatcaa acttggtatt attccatctc ttgc
//
LOCUS       DANLUA010000332          650 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.338, whole genome shotgun sequence.
ACCESSION   DANLUA010000332 DANLUA010000000
VERSION     DANLUA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.338"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>650
                     /locus_tag="PXB44_002639"
     CDS             <1..>650
                     /locus_tag="PXB44_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5283055.1"
                     /translation="ELLSLEGFKMKVVHNGLEGLNELESNQYDLVLLDVMMPVLSGVK
                     TLKRIRQKYSLPVLMLSARDDQIDRILGLELGADDYVPKPFNNRELVARMKAILRRTT
                     SRPASTDHSEQNYTENISKSLQFAGVELHSGRQQAYYQGCDLDLTGTEFALLQMLMRN
                     SGEILSRELLSLKILGKNLTPYDRAIDMHISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          224 a           95 c          130 g          201 t
ORIGIN      
        1 ctgaattact ttcattagaa ggatttaaaa tgaaagttgt gcataatggt ttagaaggct
       61 taaatgagtt agagtctaat caatatgatt tagtattact agatgtgatg atgccggtat
      121 taagtggcgt gaaaacatta aaacgtatac gacaaaaata ctcattaccc gttttaatgt
      181 taagtgcaag agatgatcaa attgatagaa tcttaggttt agaattaggg gctgacgatt
      241 atgtaccgaa accttttaat aatcgtgaac tagtcgctag aatgaaagcg atacttcgaa
      301 gaacaacctc tcgcccagct tcaacagacc attctgaaca gaattatact gaaaacatct
      361 ctaaatcatt acaatttgcc ggagttgaat tacattcagg acgtcagcag gcttattatc
      421 aaggatgtga tcttgattta acggggactg aatttgcatt attacaaatg ttaatgcgaa
      481 actcagggga gattttgtcc agagaattat tgagtttaaa aattttaggg aaaaatttaa
      541 cgccttatga tcgtgcaatt gatatgcata tttctaatct tcgtaaaaaa ttacccgaac
      601 gggaagatgg attaccttgg tttaaaacct taagaggtcg gggttactta
//
LOCUS       DANLUA010000333          648 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.339, whole genome shotgun sequence.
ACCESSION   DANLUA010000333 DANLUA010000000
VERSION     DANLUA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.339"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(19..303)
                     /locus_tag="PXB44_002640"
     CDS             complement(19..303)
                     /locus_tag="PXB44_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283056.1"
                     /translation="MANITLSHITPKHFFETVRLLKMQEHRIYIGVDSIEVRFKQKRF
                     ESKKETDKRLEQLEHFMLFLQEIINSDIPIDLDEFIYTYHRSVRRGFYRR"
     gene            complement(321..482)
                     /locus_tag="PXB44_002641"
     CDS             complement(321..482)
                     /locus_tag="PXB44_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL5283057.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          202 a          112 c          103 g          231 t
ORIGIN      
        1 cactcctctt tcacatcact aacgtcgata aaaacctctc cttactgacc tgtgataggt
       61 gtatataaac tcatctaaat ctataggaat atctgaatta atgatttctt gtagaaagag
      121 cataaaatgc tctaactgtt ctagtctctt atctgtttcc ttttttgact caaaacgctt
      181 ttgcttaaaa cgaacttcta tcgaatctac accgatgtaa attcgatgtt cctgcatttt
      241 gagtagtcgg acagtttcaa aaaaatgttt aggggtgatg tgagaaaggg ttatatttgc
      301 catagtttag cttgaaaacg ctatttatta cttgttttag caacatacaa agaatacttt
      361 tttaaagcct ctagcattac taaattaggc tctttaggat gttctaagct ctcaagaatt
      421 agcatttgat tttcaagaga aagctttaga acaagatttg aactattctc tattttttcc
      481 attttaatta tctcattaga tgactttcgc ataacaacag gttatgtgta aattctcgtg
      541 cagtgggaca agcccgattg tactgcactc gaattgtaac ataacctgta ggacattatg
      601 cgaaaattaa taatataagt ttttcatcaa tcggcggtat tattgaat
//
LOCUS       DANLUA010000334          648 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.340, whole genome shotgun sequence.
ACCESSION   DANLUA010000334 DANLUA010000000
VERSION     DANLUA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.340"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(186..>648)
                     /locus_tag="PXB44_002642"
     CDS             complement(186..>648)
                     /locus_tag="PXB44_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283058.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTRAFDAAVKAFENAFNAKD
                     TAENTAPVESKETEQENKQETTKLVITKPTLKGSEKQIDSANEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDNSEPKQFWLSVKYGW"
BASE COUNT          147 a          128 c          119 g          254 t
ORIGIN      
        1 cttatagggt atctcaagat tattgtctgt acgtttgttg gcacatcgtc accataagta
       61 gcagcgacaa tttgcccgat aatgcgcata atttgtggtg ttacttgtgt agtcataata
      121 atctccagtt atctgtttgt tttagccgta agcctttgct cttacggctt atttgttatc
      181 tcaatctacc atccatattt tacggataac caaaattgct ttggttcgct attatcattt
      241 agacctttaa atttttcgat ccactttcca gcgtttgtca ttgcaaaaaa attatctacc
      301 attgcttgag cttgagtaat ttgttcttgc gttgctgtta ctgggataga ggtatcacca
      361 atcttcgcaa taaaaagatt gatgatttcg tttgccgaat caatttgttt ttcagagcct
      421 tttaatgttg gttttgtgat tacaagctta gttgtttctt gtttgttctc ttgctctgtt
      481 tctttgcttt ctaccggcgc tgtattttca gcggtgtctt tagcattaaa agcgttttca
      541 aaagctttta cggcagcatc aaacgctctg gttgttaatc ctttgccaag tttgctcact
      601 aactcgcctg tggctaggtc aatgtacaac tggtaattcc gatcacct
//
LOCUS       DANLUA010000335          644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.341, whole genome shotgun sequence.
ACCESSION   DANLUA010000335 DANLUA010000000
VERSION     DANLUA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.341"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>644
                     /locus_tag="PXB44_002643"
     CDS             <1..>644
                     /locus_tag="PXB44_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5283059.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAKGFGRGLMSAGVGIAAGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRI
                     EGKKELLAAVQEAVKVGGGTKEDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGA
                     SSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGNFELADMARWLPQQMAAG"
BASE COUNT          184 a          129 c          190 g          141 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctctcaa
       61 aatgagctac gccgtgcctc agaagccact aagcaacggg tgcgagagct gaatgcagaa
      121 ttgggtaaga caagctttgg ggataaggct aaaggatttg ggcgtggttt gatgagtgcc
      181 ggtgtcggta ttgcagcagg tgctgcaatg gctcgtcagc cagccaagaa ccaaatggag
      241 tttgaccgac agttagcgat ggcatcaaac actgcctttt ccgaccgtga tgttaaaggg
      301 cgtattgagg gcaagaaaga gttattagca gcggtgcaag aggcggttaa ggttgggggt
      361 ggaacaaaag aagatgctct tttaactctt gatacgctaa ttgcctcagg aacgatgaag
      421 gctgaaactg caatgagcct attaccaacc ttgcagaaag gtgctgtagc aacaggtgca
      481 agctccgagg atatggcaaa aattgcgatc tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg ggcgggcatt ggatatggca gttgcagccg ggcaagcagg taactttgag
      601 cttgcagata tggctcgctg gctaccacaa caaatggccg cagg
//
LOCUS       DANLUA010000336          642 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.342, whole genome shotgun sequence.
ACCESSION   DANLUA010000336 DANLUA010000000
VERSION     DANLUA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.342"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(90..>642)
                     /locus_tag="PXB44_002644"
     CDS             complement(90..>642)
                     /locus_tag="PXB44_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5283060.1"
                     /translation="ILNNNILLPDFLKPSWFILAIIMTLRCTGIRRGQLVKLKIGDVN
                     LSNRTIYISPEINKNHDYHIVPISDSLYLHIERLITELKIRKQTDNCQLFNLNLFSRC
                     SRRRGQPMSQNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVQKLLGHK
                     DISVTLGYIEHDVEMLRETVNLL"
BASE COUNT          209 a          106 c          106 g          221 t
ORIGIN      
        1 ataaaatcag ttcatttaga agataaaaca gaaattattt atttatagaa aaaaagtggg
       61 agaaattata ttctcccatt tttcttttat cagagtaaat tcactgtctc tctcaacatt
      121 tccacatcgt gctcaatata acctagtgtt acgctgatat ccttatgccc tagaagtttc
      181 tgaaccacat aaacattttc gggcttcttc attaattgag ttgcaaccgt atgtcgaaat
      241 ctatgtggtg atgaagtaaa accgaccaag tcagaaatat gccgaaatat atgcgtaagc
      301 tgattttggc tcattggctg tcctcttctt cgagagcatc gagaaaataa attgagattg
      361 aacaattggc aattatctgt ttgttttctg atttttaact ctgtaataag cctttctata
      421 tgaagataaa gtgaatctga aatcggtacg atatggtaat catgattttt attgatttct
      481 ggcgaaatat aaatcgtcct gttggaaaga tttacatcac caattttcag tttcactaat
      541 tgtcctcgcc gaatgcctgt acatctcaat gtcataataa tcgcaagaat aaaccaactt
      601 ggttttagaa aatcaggtaa tagaatgtta ttattgagta tg
//
LOCUS       DANLUA010000337          616 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.344, whole genome shotgun sequence.
ACCESSION   DANLUA010000337 DANLUA010000000
VERSION     DANLUA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.344"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(378..453)
                     /locus_tag="PXB44_002645"
     tRNA            complement(378..453)
                     /locus_tag="PXB44_002645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Ala,seq:tgc)
     gene            complement(510..586)
                     /locus_tag="PXB44_002646"
     tRNA            complement(510..586)
                     /locus_tag="PXB44_002646"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Ile,seq:gat)
BASE COUNT          160 a          148 c          106 g          202 t
ORIGIN      
        1 gcacgtcctt cttcgcctct gattgccaag gcatccacct tgtgcgctta gtcacttaac
       61 tatacaacct caaatgtttt ctaaaacttc tcaaaaataa aagtaaaaaa acaatgaagt
      121 cagttttaca actaaacact tgactgcttt tgttcagtca agattttttc tactcagact
      181 ttcaatttat tcgttatcac ttatgtataa ttacttatgc gtaacgaact tgaaatatct
      241 ctcagttttt cagcttgttt ccatattgtt aaagaacaaa gataatggct ttcgcctatc
      301 atcataccta aaattgcaaa taatccggta tttttaaccg cttgtaatct taggtatgat
      361 gataaagtca cgatgattgg tggagataag cgggatcgaa ccgctgacct cctgcgtgca
      421 aggcaggcgc tctcccagct gagctatatc cccatttcat cttccgtttt cttcactcat
      481 agtcgcttcg ctttcgcttg ccttctgagt ggtgggtctg agtggacttg aaccaccgac
      541 ctcaccctta tcaggggtgc gctctaacca cctgagctac agacccaacg gatgaccttc
      601 ggttttttgt cttctt
//
LOCUS       DANLUA010000338          607 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.345, whole genome shotgun sequence.
ACCESSION   DANLUA010000338 DANLUA010000000
VERSION     DANLUA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.345"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(25..>607)
                     /locus_tag="PXB44_002647"
     CDS             complement(25..>607)
                     /locus_tag="PXB44_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283061.1"
                     /translation="FNFFPEFITILGTGAFNGFLDFPSLVGKVFPEIIDTINEGLDLD
                     IAIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGLLNVDEINSFYEDNSELILLAKQ
                     PMVEEIIPKPDSKASSAILKFFDSNSNILIAKLKESGLQLSAVALQNDQNIARVANVV
                     YNLLPGMVRIFVSYDTVENFLLNNRQWLINKLV"
BASE COUNT          200 a          122 c           80 g          205 t
ORIGIN      
        1 tatccatagt tcttcccctc caaattaaac taatttatta atcaaccatt ggcggttatt
       61 taataagaaa ttttctacgg tatcgtaact tacgaaaatt cttaccatgc ccggcaataa
      121 gttataaacg acatttgcta ctcgtgcgat attttggtcg ttttgtaatg ccacagcact
      181 taattgcaaa ccactttctt ttaatttagc aattaagata tttgagtttg aatcaaagaa
      241 ttttagaatt gcagaggagg ctttgctatc tggtttaggg attatctctt caaccatagg
      301 ttgtttagca agtaagatta gctcactatt atcttcataa aagctattta tttcatcaac
      361 atttaataaa ccaatttcgc cttcttcagc tttcttaatg agagcctgct catataaacc
      421 catacattca gtcatttctt cctgactcaa cacttcaaag ttctcaatcg caatatctaa
      481 atctaaaccc tcatttatcg tatcaataat ttctggaaaa acctttccta ccaaactagg
      541 gaagtctaaa aatccattaa acgcaccagt cccgagaata gtgataaatt cgggaaaaaa
      601 gttaaaa
//
LOCUS       DANLUA010000339          593 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.346, whole genome shotgun sequence.
ACCESSION   DANLUA010000339 DANLUA010000000
VERSION     DANLUA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.346"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>593)
                     /locus_tag="PXB44_002648"
     CDS             complement(<1..>593)
                     /locus_tag="PXB44_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5283062.1"
                     /translation="RILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRW
                     KRVDLSHLTLFDFWAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVKI
                     LGNKTGKLVLGAGIQQAGAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNL
                     HIDNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQS"
BASE COUNT          193 a          150 c           89 g          161 t
ORIGIN      
        1 gattggcagt tttgtacaat cacattatca gttgggtaat tagtatccgc ctgaatactt
       61 tcacctgccc tgaccccgta gaaggtgcca ttaaacacaa cattgtcaat atggagattt
      121 ttaacaccag catcatccaa agacttaaat cgaataccaa ttgctttgtt cggtgtttca
      181 atacaaaaac ccgagataaa ataattgctg tgattaatat taaaaacttc ggcccctgct
      241 tgctggatac ctgcacctaa tactaatttg ccggtcttat tgcccaaaat tttcacatta
      301 tcgcgaacag aattaacggc attgttgata cgaaatgtac cgttttcgat aaaaaattga
      361 gaatatctca tagcatttac aaatgctgat tcatcatttg ttaccccgtc accgacggcc
      421 caaaaatcaa ataatgtaag atgtgaaagg tcaacacgct tccaccgccc cccattatta
      481 ttcacaacaa aacaaacccc accatcatca ggcgtaacta aatcctgcaa atcagccaca
      541 aactccccac cgcctgttgt gccgtcctca tagtaagcat ccaccaaaat acg
//
LOCUS       DANLUA010000340          591 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.347, whole genome shotgun sequence.
ACCESSION   DANLUA010000340 DANLUA010000000
VERSION     DANLUA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.347"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..585)
                     /locus_tag="PXB44_002649"
     CDS             complement(<1..585)
                     /locus_tag="PXB44_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5283063.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALK"
BASE COUNT          204 a          105 c          123 g          159 t
ORIGIN      
        1 ttttaatgca ccaaatttaa agcgttcgca ttgcccattt gcacattcat gtgttttggg
       61 tgcggtttta aacataacga atagcataat aatttcaatg cagcagctaa tccaaataag
      121 ccattctgct ttacggtaga aaataaatgg aatagtaagt aatggtgcta ccatcgctga
      181 caggcttgat accttcagaa atttaccttg agccttagtt ttgagttggt aactatctcc
      241 ggtatcttga atattggcta ataagaatgc tctcgcattg gtactaaata aggtgctacc
      301 gagcccgaag aaaaaggtag caagtaatag cagagggtaa ttttttgttg tcagtaataa
      361 aatgtaagct agggcatcga gcaaacagcc aagcatcatg atagggtaac gtccaaaact
      421 atcaccccaa gaacctgcaa aaagggaaaa tgcttggctg gtaaagacca acaaagaaaa
      481 tgcagttgca atttggctgg tttcaaagcc tttgtagaat gtaagtagat aaaaaatacg
      541 ctttgcattg cagcataagc aagaccggaa aagaatttcc gccataaaat g
//
LOCUS       DANLUA010000341          581 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.348, whole genome shotgun sequence.
ACCESSION   DANLUA010000341 DANLUA010000000
VERSION     DANLUA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.348"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..316
                     /locus_tag="PXB44_002650"
     CDS             <1..316
                     /locus_tag="PXB44_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HDL5283064.1"
                     /translation="HIVDGNLFLVTPVIFEMYLQECGQPYDKETINNLQYDFQGLGLH
                     SLKKTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTHLLFGDKMLLNNHCLTLQEE
                     DT"
     gene            356..>581
                     /locus_tag="PXB44_002651"
     CDS             356..>581
                     /locus_tag="PXB44_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HDL5283065.1"
                     /translation="MFFYRHYLRADTKRSYNNIIRIIKKRYPNLNANDVTTDMLIEWR
                     NQVVGNTIKAVTWNNYVRHLKALYNFAIAQG"
BASE COUNT          201 a           83 c          105 g          192 t
ORIGIN      
        1 acatatagta gatggtaatt tatttttagt cacaccagtt atttttgaaa tgtatttgca
       61 agaatgtggc caaccttatg ataaggaaac tataaacaat cttcagtatg atttccaggg
      121 gcttggtctt cattctctta aaaaaaccat gactaatggt aagccagata caataaattt
      181 ttggaagtgt gacgtgattg gtcctagaaa gacttcctct ttaacaggat atttgataaa
      241 aaatactcat ctcttatttg gggataaaat gttactaaat aaccactgtt taactttaca
      301 ggaagaggac acataatatg gattctaaga tttccttcga tatattattg gaacgatttt
      361 tttctatcgt cattatttac gggcggacac gaaacgaagt tataacaata ttattcgaat
      421 tatcaaaaag cgttatccaa acttaaatgc gaatgatgtg actactgata tgttaattga
      481 atggcgtaat caagttgttg gtaatacaat taaagctgtg acgtggaata attatgtgag
      541 acacctaaaa gcattatata attttgcgat agcacagggg c
//
LOCUS       DANLUA010000342          581 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.349, whole genome shotgun sequence.
ACCESSION   DANLUA010000342 DANLUA010000000
VERSION     DANLUA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.349"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..298
                     /locus_tag="PXB44_002652"
     CDS             <1..298
                     /locus_tag="PXB44_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5283066.1"
                     /translation="ACLIYLRKFGYSLGRPYADTVYQSKFPNMKELRIQNKGKPIRAF
                     YAFDPLRQAIVLCAGEKGKDKRFYEKMIALADAEFTSYLNNLEKENENVRSNAQ"
     gene            270..569
                     /locus_tag="PXB44_002653"
     CDS             270..569
                     /locus_tag="PXB44_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5283067.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
BASE COUNT          201 a          107 c          111 g          162 t
ORIGIN      
        1 ggcttgttta atctatctgc ggaaattcgg ttatagtcta ggcagacctt atgctgatac
       61 agtttatcag tctaaattcc ctaatatgaa agagttacgc attcaaaaca aaggtaagcc
      121 aattcgagca ttttatgctt ttgacccttt acgccaagcg atagtacttt gtgctggcga
      181 gaaaggtaaa gacaaaagat tctatgaaaa aatgatcgct cttgctgatg cggaatttac
      241 ctcctattta aataatttag agaaagaaaa tgaaaacgtt agatcaaatg ctcaatgaac
      301 tctctcctga acgccgccaa aaagtggaag atatggctca agaaatgatt agagagatac
      361 aactttcaaa actaagagaa gagctaaatc tctctcaaca acaattagct acagctctcg
      421 gtatttcaca gcctgctatt gctaaacttg aacagaaaga taacgatcca agattatcca
      481 cgctcaaacg ctatattgaa gcaatgggcg gtaagttaaa acttgccgtt gaattgccta
      541 caggcgatga gcgtgtaatt aggatttaat tactttgtta a
//
LOCUS       DANLUA010000343          578 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.350, whole genome shotgun sequence.
ACCESSION   DANLUA010000343 DANLUA010000000
VERSION     DANLUA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.350"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>578
                     /locus_tag="PXB44_002654"
     CDS             <1..>578
                     /locus_tag="PXB44_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5283068.1"
                     /translation="FSRCNPEKGDILYIKDGATTGVSIINTLNEPFSMLSSVALIKTS
                     QEIDNEYLNYVMNSHYFYNISIGSMSGTGIPRITLTKLESYLVPVPPLLEQQRIVQKI
                     EELLPLVERYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERI
                     KAEKNRLIAEKKLKKPKSVSEIVMRDNLPYEI"
BASE COUNT          212 a          104 c          103 g          159 t
ORIGIN      
        1 ttcagccgtt gtaatcctga aaagggagat attctatata ttaaagatgg agccacaact
       61 ggagtaagta ttataaatac tcttaatgag ccatttagta tgttatccag tgttgcctta
      121 attaagacct cacaagaaat agataatgag tacttaaatt atgtaatgaa tagtcattat
      181 ttttataaca tttctattgg aagtatgtct ggaacaggta ttccaagaat aacattaact
      241 aaattagaat cttatttagt cccagttccg ccactcctag aacaacagcg cattgtgcaa
      301 aaaatcgagg aattattacc gcttgttgaa cgctacgagc aaaccgaaca gcaactcact
      361 aaactcaaca atacattccc cgaacagctg aaaaaatcgg tgttacacgc agctattcag
      421 ggtaaattaa ccgagcaaga tccgaatgat gaactggcat cttgcctgat tgagcggata
      481 aaagcggaga aaaatcgtct tattgccgag aaaaaactca aaaaaccaaa aagtgtttca
      541 gagattgtaa tgcgtgataa tttaccttat gagatcaa
//
LOCUS       DANLUA010000344          577 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.351, whole genome shotgun sequence.
ACCESSION   DANLUA010000344 DANLUA010000000
VERSION     DANLUA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.351"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..554)
                     /locus_tag="PXB44_002655"
     CDS             complement(<1..554)
                     /locus_tag="PXB44_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5283069.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTL"
BASE COUNT          231 a           77 c          103 g          166 t
ORIGIN      
        1 agagttaaaa ataaaacagg gaatgcccaa aaactagaaa aaatgccaaa tttttcaaaa
       61 atatgaaagt tgttttgttt atttaaatca aataaagcaa taatggctat ggtaatagcg
      121 cttaatgttg caaaaataat gccagataaa actgtagcaa agacaatgtt aactaattta
      181 ttagttgttc cggctacaaa ataatggttg tttttgtacc aatattgact gatcaaacaa
      241 aggaatacac aaaatatggt agcccaataa cgagaatctt ccaataatgt taaattaatt
      301 tgaccaaaga aaaagacgat tgctatcgga atcggagcga atagccaata agctttggta
      361 tcgcgtatca ggtaaagcaa aataaagcaa ataggaaaaa gagcaattag attttgaaag
      421 cgtttgatta attcgtagtc gctcacatta ttgataagta aaatcgtgac gtaagtaacg
      481 aataaaattt ccagtggatg cttaatgatg gtgcttttta tcattaaggt aaagcgttgt
      541 agcttactta gcataataga tctcctgcct atgaaaa
//
LOCUS       DANLUA010000345          577 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.352, whole genome shotgun sequence.
ACCESSION   DANLUA010000345 DANLUA010000000
VERSION     DANLUA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.352"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..534)
                     /locus_tag="PXB44_002656"
     CDS             complement(<1..534)
                     /locus_tag="PXB44_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283070.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFFQLSIFYLMLLHFLL"
BASE COUNT          233 a          102 c           87 g          155 t
ORIGIN      
        1 caataaaaaa tgtaatagca ttaaataaaa aatgcttaat tggaaaaagg cggaagaagt
       61 gatgttatat aaacggtatt cttgttccga accaaaaaaa cgtggcatca ttgcattgct
      121 ggataataaa aaatccacca taaacggcaa acctaataag gctgtcacac cacaaatcca
      181 ttgaatagca atgccgattt ttaaatcacg aacgaataaa gttttacctt gttggttatg
      241 ataaaaaaca ttataaatcg caatcccgaa cacaaacaaa atcagcattt tgacatacat
      301 tgccagcaca ccaatttctt ttgtactgtt aatcaataat ggaataatgc taaagccgat
      361 taatgcaata attgcaatca ggctatcttt aggaataata atgccattcg atttgccctt
      421 gattatttgg aaaaccaaat agccacaagc aacaagcccg actacaaacg acattcttaa
      481 aaaatggaaa aaccacggat cgtaaagata aaataagtta atgagtgctg acatttcaga
      541 cctattatga atttcgctga gtaaaattac gtttaat
//
LOCUS       DANLUA010000346          567 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.353, whole genome shotgun sequence.
ACCESSION   DANLUA010000346 DANLUA010000000
VERSION     DANLUA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.353"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..490
                     /locus_tag="PXB44_002657"
     CDS             <1..490
                     /locus_tag="PXB44_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5283071.1"
                     /translation="IMTLRCTGIRRGQLVKLKIGDVNLSNRTIYISPEINKNHDYHIV
                     PISDSLYPHIERLIQELKIHKQTDDCQLFNLNLFSRYTRRKGQPMSQNQLTHIFRHIS
                     DLVGFTSSPHRFRHTVATQLMKKPENVYVVKKLLGHKDISVTLGYIEYDVEMLRDTVN
                     LL"
BASE COUNT          201 a           91 c          101 g          174 t
ORIGIN      
        1 cattatgaca ttgagatgca caggaattag gagagggcaa ttggttaaac tgaaaatcgg
       61 tgatgttaat ttatcaaata ggacgatcta tatttcacca gaaattaata aaaaccatga
      121 ttatcatata gtaccgatat ccgattcact ttatccccat atagaaaggc tgattcagga
      181 gttaaagatc cataaacaaa cggatgattg ccagttattt aacctcaatt tattttctcg
      241 ttatactcgt aggaaaggtc agccgatgag ccaaaaccag cttactcata tatttaggca
      301 tatttccgat ttggttggct tcacgtcatc accacataga tttcggcata cagtagctac
      361 tcaattgatg aagaaacctg aaaatgttta tgtagttaag aaattattag gtcataaaga
      421 tatcagcgtt acattaggat atattgaata cgatgtggaa atgctacgag acaccgtgaa
      481 cttactctaa taaaagagaa atgagagaaa actattctct catttattgt gataaatagg
      541 aaacttctgt cttattttca gatgaat
//
LOCUS       DANLUA010000347          565 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.354, whole genome shotgun sequence.
ACCESSION   DANLUA010000347 DANLUA010000000
VERSION     DANLUA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.354"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..285
                     /locus_tag="PXB44_002658"
     CDS             <1..285
                     /locus_tag="PXB44_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283072.1"
                     /translation="KNQNSQENNLTEINSNIQILAEKHQLTIESLQWNLEQGKSIELS
                     IIGNSRSLFDFIHSVKQIPYLKYNAISLIKSRQDRKVEMNTTLVILANKE"
     gene            288..>565
                     /locus_tag="PXB44_002659"
     CDS             288..>565
                     /locus_tag="PXB44_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283073.1"
                     /translation="MRNWFLFITLFISFSVKANDPFFGEKQETQASGNIAESNVEFAK
                     NTENITACKVPDNLSAKNIVTDFDKMTLIGLVKINEVMRALFIDEKQQ"
BASE COUNT          229 a           87 c           85 g          164 t
ORIGIN      
        1 aaaaatcaga acagccaaga gaataattta accgaaataa acagcaacat acaaatatta
       61 gctgaaaaac accaactaac cattgaaagt ttacaatgga atttagaaca aggtaaaagt
      121 attgaattga gtattatcgg caatagccga tctttatttg attttatcca ttcagttaag
      181 caaatacctt atttgaaata caatgcgatt agcctcatca aatctcggca agacagaaaa
      241 gttgaaatga atactacctt agtcatatta gctaataagg aataacaatg cgtaattggt
      301 ttttatttat tactttattt atcagttttt ctgtaaaagc aaatgatcct ttctttggag
      361 aaaaacagga aactcaagcc tcaggcaaca tcgctgaaag caatgtggaa tttgcaaaaa
      421 atactgaaaa catcaccgct tgtaaagtac ctgataacct ttctgcaaaa aatatagtaa
      481 ctgattttga caaaatgaca ctgataggat tagttaaaat caatgaagta atgcgtgcat
      541 tatttattga tgaaaaacaa caatt
//
LOCUS       DANLUA010000348          565 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.355, whole genome shotgun sequence.
ACCESSION   DANLUA010000348 DANLUA010000000
VERSION     DANLUA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.355"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>565
                     /locus_tag="PXB44_002660"
     CDS             <1..>565
                     /locus_tag="PXB44_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5283074.1"
                     /translation="AINTMSSDWEALNNHFDLALFEQRYGRKTTKGEVGCTLSHLAVY
                     QKIAEDSTIAEDDYCLVCEDDALFADDFQKHLDALLAQNLKVDIILVGQSKIPTFNDS
                     ELELNYPSTFGFLQKNIPNSQYKYAYSYLNYFAGTVAYLIKKSTACQFLAQCAGKNPY
                     WLADDFILFEKNFKFDVLIVRPLMAIEN"
BASE COUNT          179 a           93 c          106 g          187 t
ORIGIN      
        1 gccattaata caatgagtag tgattgggag gcattaaata accattttga tttggctctg
       61 tttgaacagc gatatgggcg taaaaccact aagggcgaag taggttgtac tttaagccat
      121 ttagccgttt accaaaaaat tgccgaagat agcactattg cggaagatga ttactgccta
      181 gtctgtgaag atgatgcctt atttgccgat gatttccaaa aacatcttga tgctttatta
      241 gcacaaaatt taaaagtcga tattatttta gttgggcagt cgaaaattcc aacttttaac
      301 gatagcgaat tagagcttaa ttacccatca acctttggct ttttgcaaaa aaatattccg
      361 aatagccaat ataaatatgc ttattcttac ctgaattatt ttgctggtac ggttgcatat
      421 ttaattaaaa aatcgaccgc ttgtcagttt ttagcacaat gtgccggaaa aaatccatac
      481 tggcttgccg atgattttat tttatttgaa aagaatttca aatttgatgt gttaattgtc
      541 cgcccgttaa tggcgataga aaatc
//
LOCUS       DANLUA010000349          561 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.356, whole genome shotgun sequence.
ACCESSION   DANLUA010000349 DANLUA010000000
VERSION     DANLUA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.356"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..296
                     /locus_tag="PXB44_002661"
     CDS             <1..296
                     /locus_tag="PXB44_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283075.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            324..>561
                     /locus_tag="PXB44_002662"
     CDS             324..>561
                     /locus_tag="PXB44_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283076.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVL"
BASE COUNT          206 a           89 c          106 g          160 t
ORIGIN      
        1 cagataccag atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg
       61 ctaaaaatcg ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt
      121 ctgagacata tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa
      181 agcaatctca accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa
      241 ctgctggggt tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta
      301 tctctaacca atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa
      361 atagtgatca ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta
      421 tagcacagtg tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta
      481 tgtatgttgg ttgtttccat acttttaaca aattatctca atctaaagct tatgttcaag
      541 agaactttcc aaacgtactg g
//
LOCUS       DANLUA010000350          539 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.357, whole genome shotgun sequence.
ACCESSION   DANLUA010000350 DANLUA010000000
VERSION     DANLUA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.357"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            83..508
                     /locus_tag="PXB44_002663"
     CDS             83..508
                     /locus_tag="PXB44_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5283077.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT          174 a           86 c          111 g          168 t
ORIGIN      
        1 tctacttcaa gtttttatca aaaagagtgt gttagtatgt ttatacacaa agtaaacaac
       61 tctcacaagc aaggagcata ttatgtataa acatatctta gttgcagtgg atctttctga
      121 agaaagtctt gttttagtgc gtaagggggc tgcgttagca gaaaaatgcg gagctaaact
      181 ttccctcatt cacgttgatg ttaatttttc ggatctctat acaggattaa ttgatattaa
      241 tatgtcttcg gtacaataca gtgttgttga agaaacaaat acggcactgg ctgaacttgc
      301 ctcaaaagta aattatcctg tatctgaatg tttaaacgga acgggagatt ttagtcaagt
      361 tttggaagag gccgtagaaa aacacaatgt cgatttactc atcactggcc atcatcagga
      421 cttctggagt aaatttatgt cttcaactcg tcgagtcatg aataatatca ctgttgatat
      481 gctggttgtg ccattagcag atgaatagca agaaagtgaa tgaaaaataa ccgcttgag
//
LOCUS       DANLUA010000351          533 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.358, whole genome shotgun sequence.
ACCESSION   DANLUA010000351 DANLUA010000000
VERSION     DANLUA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.358"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>533
                     /locus_tag="PXB44_002664"
     CDS             <1..>533
                     /locus_tag="PXB44_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283078.1"
                     /translation="KISHCTLLNACSKALGFSGGFAGFQEDYLSNLTQFMEKHGLLYL
                     VDLTKQKYPVNDMCMTRSLALKKQDISERIFFSGKEIPKRIFTGFNFRYDMHLANGFR
                     IGNSLKTPYSDIGICEDINEALKNPSIKIDCRDGTIRSMADVVIGRYLDKVLFYYFNL
                     IGDQLVQPRLDKYEIQC"
BASE COUNT          190 a           71 c           97 g          175 t
ORIGIN      
        1 aaaatttctc actgtacttt gttaaatgct tgctcaaaag ctttaggatt ttctggaggc
       61 tttgcagggt ttcaagaaga ttatttatct aatttgacgc aattcatgga aaaacatggg
      121 ctcttatatc ttgtagattt aacaaaacaa aaatatccag taaatgatat gtgcatgaca
      181 aggagcttag ctctgaaaaa gcaagatata tctgaaagaa ttttctttag cggaaaggaa
      241 atccctaaga gaatatttac tggatttaat ttccgatatg atatgcattt agctaatggt
      301 tttcgtatcg gaaattcctt aaaaacccca tattctgaca taggtatatg tgaagacata
      361 aatgaagcat taaaaaatcc atcaataaaa attgattgcc gtgatggcac aatcagaagt
      421 atggcagatg tagtcatagg tagatatctt gataaagtat tgttttatta ttttaacttg
      481 ataggtgatc agttagtcca gcctagattg gataaatatg aaatacaatg tta
//
LOCUS       DANLUA010000352          532 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.359, whole genome shotgun sequence.
ACCESSION   DANLUA010000352 DANLUA010000000
VERSION     DANLUA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.359"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            90..>532
                     /locus_tag="PXB44_002665"
     CDS             90..>532
                     /locus_tag="PXB44_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283079.1"
                     /translation="MELGKTTETDVKEMYNLEPEGVNKYSKGNQYFIDPTQLDLEDLK
                     STLIIFDEKGVLVFVGSEFNSISSIEDSKNRYKKFDYLYKILASKYKLVKKERPFVGD
                     QYAKFKDGNSEIELSEPHMGGFKINLTYAKKEFLDAFQKIQSEDK"
BASE COUNT          213 a           71 c           88 g          160 t
ORIGIN      
        1 tataggaaac tcattatgaa aaagatgtta aagctacatt atttgcaact attagtactt
       61 ctgtattagc tgaaccaacc gtttttaaaa tggaactagg taaaacaaca gaaacagatg
      121 taaaagaaat gtataacctt gaaccggagg gtgttaataa atacagcaag ggtaatcagt
      181 attttattga tccaacgcaa ttagatttag aagatcttaa atcaacgctt attatctttg
      241 atgaaaaagg ggtattagtt tttgttggtt ccgagtttaa ttctatatct tctatcgagg
      301 attcaaaaaa ccggtataaa aaattcgatt atttgtacaa aatactagca agcaaatata
      361 aacttgtaaa aaaagaacgt ccatttgtag gcgatcaata tgctaaattt aaagacggta
      421 attcagaaat cgaattatca gaaccacata tgggtggttt taaaattaac ttgacttatg
      481 caaaaaaaga attcttagac gcattccaaa agatacagtc tgaagataaa aa
//
LOCUS       DANLUA010000353          529 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.360, whole genome shotgun sequence.
ACCESSION   DANLUA010000353 DANLUA010000000
VERSION     DANLUA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.360"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>529
                     /locus_tag="PXB44_002666"
     CDS             <1..>529
                     /locus_tag="PXB44_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5283080.1"
                     /translation="QGNLVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGIDDWENS
                     NFNFRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNYHLVVAK
                     MSYENLKKELSKKPDSTFNKLKKLYETNGEFLNEYILDNFRGNPLELLTVPALKESLE
                     NQESFPSRLYQKFNIF"
BASE COUNT          192 a           89 c           84 g          164 t
ORIGIN      
        1 tcaaggtaac ttagtaacag ccattcctaa cttcctggag aaagttaata atgattttgc
       61 tataagtgct agaaatagag acttatatag tcactacctt ccttatcagg gtattgatga
      121 ttgggagaat tctaatttta actttcgaac actaaatgga gtttattatg atgataatct
      181 aacttcaaca gaactcctgc gaaaatttat gattcaaaga gattataaca ctctttatgc
      241 tctaagttct gctcgcacag gaaatgctga tagtctggct aaaaattacc atcttgtagt
      301 tgcgaaaatg tcctatgaaa acctgaaaaa agaattatcc aagaaacccg actctacatt
      361 taataaactg aaaaagctgt atgaaaccaa tggtgaattc ttaaatgaat atattttaga
      421 caatttccga ggaaatccac ttgaattatt aacagtacct gcgcttaaag aaagtttaga
      481 aaaccaagag tcattcccta gcaggctgta tcagaaattt aatattttc
//
LOCUS       DANLUA010000354          516 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.361, whole genome shotgun sequence.
ACCESSION   DANLUA010000354 DANLUA010000000
VERSION     DANLUA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.361"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          159 a           83 c           93 g          181 t
ORIGIN      
        1 tcaaataaaa agtgagctaa aaaagatttt aactcacttt catttcgtag gctcaaatta
       61 gacctactac gccgatataa aaagttggtc tttttatatc aaagctaagc tcttaacaac
      121 gagtagtaga ttctctaata tgtttagctg taactttcat tggcttttgt tgcgctgtag
      181 tgtttttcat attgttctcc agattttgtg agtaattagt aagacctttt cttttgaaaa
      241 ggaccgagct ttcaaagtgg tctgattacc tattgagcaa tcagcttgct tttcaagccc
      301 catgtcatta tatcataacc attgttttta gtcaatacca ttttggggaa tgcatggaag
      361 gcgagtacaa attgaaggct gatgtcacga tctatcatga tacgccttat acaaaagttc
      421 tctttggaag tacatatatt gagattttag acgatgacca gtattacttt tcgattttag
      481 aaaaacgaag atggaagtta gaaaatttac ctgatg
//
LOCUS       DANLUA010000355          505 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.362, whole genome shotgun sequence.
ACCESSION   DANLUA010000355 DANLUA010000000
VERSION     DANLUA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.362"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(186..>505)
                     /locus_tag="PXB44_002667"
     CDS             complement(186..>505)
                     /locus_tag="PXB44_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL5283081.1"
                     /translation="AKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIGNYIVDFVC
                     FSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAVLDTIFYEI
                     TRG"
BASE COUNT          172 a           95 c           76 g          162 t
ORIGIN      
        1 cccacaaggg gagagggtta ttctttcaaa aattcaaaac aactaaaaaa ttagcattct
       61 ccttttgtag gagaaaatgt tgttctttca aaattctaaa ataaccgcaa aattaactct
      121 cgccccttgt gggagagaga cagataaatc attcgttcag aatgatttat cagagagagg
      181 ggaaattacc ctctcgtaat ttcataaaaa attgtatcta aaacagcctc tagatcgcta
      241 tgaatttgat tattccaaaa tcttaaaacc ctgaacccct gaccgtttaa aaaatgagtt
      301 cgtagattat catagttttg ttgatctaaa tgttgtgaac catctaattc aataactaat
      361 ttcgctgaaa agcaaacaaa atcgacaata tagttaccta taacttgttg ccgtttaaat
      421 tttatcgcta aaaatcgttt tgctcttaat gcttgccaaa gaaccttctc tgcttctgtc
      481 atattagtcc gaagctgttt agctc
//
LOCUS       DANLUA010000356          497 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.363, whole genome shotgun sequence.
ACCESSION   DANLUA010000356 DANLUA010000000
VERSION     DANLUA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.363"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..495
                     /locus_tag="PXB44_002668"
     CDS             <1..495
                     /locus_tag="PXB44_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5283082.1"
                     /translation="CLKKDNELYWYFVIRFMAATGSRVSELIQIKCEHIKIGYLDLYS
                     KGGKLRRIYIPKSLQKECSEWLRLNNKHNGFIFLNKYGDRITTRGIASQLKVFARRYK
                     LDPKIVYPHSFRHRFAKSFLERFNDIAFLADLMGHESIETTRIYLRKTSTEQQEIVNK
                     IIDW"
BASE COUNT          184 a           68 c           87 g          158 t
ORIGIN      
        1 tgcctaaaaa aagataacga actatactgg tattttgtga ttcgttttat ggcagcaaca
       61 ggttcgagag taagtgaatt aatccagata aaatgtgaac atattaaaat aggctattta
      121 gacctctatt ctaaaggtgg aaaattaaga cgaatttaca tcccaaaatc tctgcaaaaa
      181 gagtgttctg aatggttgag attaaataat aagcataatg gatttatttt cttaaataaa
      241 tatggtgata gaattactac tcgtggcatt gccagccaat taaaagtatt tgctagacgc
      301 tataaattag atcctaaaat tgtttatcca cattcatttc gccatcgttt tgcaaaaagt
      361 tttttagaac gatttaatga tattgctttt cttgctgatt taatggggca cgaaagcata
      421 gaaaccacaa ggatctattt acgaaaaaca agcacagaac agcaggaaat tgtgaataaa
      481 attattgatt ggtaatt
//
LOCUS       DANLUA010000357          496 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.364, whole genome shotgun sequence.
ACCESSION   DANLUA010000357 DANLUA010000000
VERSION     DANLUA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.364"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(124..417)
                     /locus_tag="PXB44_002669"
     CDS             complement(124..417)
                     /locus_tag="PXB44_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283083.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKEITDAPLTKEDIGKTPTQIMLGRIYKYLKETGQIVV"
BASE COUNT          135 a           97 c           90 g          174 t
ORIGIN      
        1 cttatagaga taacccctct atattaccta ccattatttc acatcttttt acattaaata
       61 acaccattta cggtacattt atggaacacg ttggtgatga gctagtcttc aaaagttact
      121 aaattaaact acaatctgcc ccgtctcttt taagtactta tagatacgcc ctaacataat
      181 ctgcgttggc gttttgccaa tatcctcttt tgttagcggt gcatcagtaa tctccttggc
      241 ggactgtgca tcaatgtatt tgtactcact tgtgatgatt acaaagtttg ttacttctcc
      301 atcggtatca tcgcctgtac cgaccacata tttagcattg agcgagccgt catcttgcgt
      361 tgagtagctt gcgatggtgg aatacattgg gtttaagatt ttgttaaaag ttgtcataat
      421 ttttcctttt gtggattgtt gataaaaaaa gaccgcttgt agtcatacaa acggcttagt
      481 aggaagagta aattgc
//
LOCUS       DANLUA010000358          489 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.365, whole genome shotgun sequence.
ACCESSION   DANLUA010000358 DANLUA010000000
VERSION     DANLUA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.365"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>489
                     /locus_tag="PXB44_002670"
     CDS             <1..>489
                     /locus_tag="PXB44_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL5283084.1"
                     /translation="VNDHIVSKYKLDRFVYPMFGKSQHCKGIIYDNEQHFENKEKNLP
                     TNFIYLEDNESNLPLLVQTYIKEGEDAEYHKRYKCKIRKPWYKVPSVYSTNIGMLKRS
                     HEAPKLILNKLGAYTTDTAYRIKSNKFQDEILVCCFLNPLTMILSELNGRFYGGGVLE
                     LV"
BASE COUNT          186 a           60 c           89 g          154 t
ORIGIN      
        1 tagttaatga tcatattgtt agcaagtata agttagatcg gtttgtttat cctatgttcg
       61 gaaaaagtca gcattgcaaa ggaatcatat acgataatga acaacatttt gaaaataaag
      121 aaaaaaattt gccaactaac tttatttatt tagaagataa tgagagtaat cttcctttac
      181 ttgtacaaac ttatataaaa gaaggtgagg atgcggagta tcacaaaaga tataaatgca
      241 aaataagaaa accatggtat aaagttccat cggtttattc aactaacata ggaatgctga
      301 aaagaagtca tgaagcacca aaactaatat tgaataaatt gggggcttat actaccgata
      361 ctgcgtatcg tataaagtca aataaatttc aagatgagat tcttgtgtgc tgtttcctaa
      421 atccattaac tatgatttta tctgagttaa atggaaggtt ttatggtgga ggcgtattag
      481 aattagttc
//
LOCUS       DANLUA010000359          479 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.366, whole genome shotgun sequence.
ACCESSION   DANLUA010000359 DANLUA010000000
VERSION     DANLUA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.366"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(116..191)
                     /locus_tag="PXB44_002671"
     tRNA            complement(116..191)
                     /locus_tag="PXB44_002671"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXB44_002672"
     tRNA            complement(195..269)
                     /locus_tag="PXB44_002672"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXB44_002673"
     tRNA            complement(313..397)
                     /locus_tag="PXB44_002673"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
BASE COUNT          149 a          110 c          109 g          111 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtca
//
LOCUS       DANLUA010000360          459 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.367, whole genome shotgun sequence.
ACCESSION   DANLUA010000360 DANLUA010000000
VERSION     DANLUA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.367"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(69..380)
                     /locus_tag="PXB44_002674"
     CDS             complement(69..380)
                     /locus_tag="PXB44_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDL5283085.1"
                     /translation="MAKSLDAKREELTNEIRDILKNAEELFNETSDSSTEEYKKLKDK
                     LAKQFDDVKDRFATLKDDTVDSARTVVKQTDTLVQDNPYKAITVAGVVGLLLGVLISR
                     R"
BASE COUNT          152 a           85 c           51 g          171 t
ORIGIN      
        1 cttatattaa ataataagcc accccaaata tggcgtggct taatgactta atgacgaatt
       61 acagataatt atcttctaga aattaaaaca cctaataata accctaccac accggcaacg
      121 gtaattgctt tatacggatt atcttgaact aaggtatcag tttgtttaac tacagttctt
      181 gcactatcaa cggtatcatc ttttaatgtt gcaaaacgat cttttacatc atcaaactgt
      241 tttgctaatt tatcttttaa ctttttatat tcctccgtag aagaatcaga tgtttcatta
      301 aataattcct ctgcattttt caaaatatcg cgaatctcgt ttgttaactc ttcacgtttt
      361 gcatctaagc ttttagccat aattgctctc cttatttaat taaacgtaat ctataaaaat
      421 aaaacataat tgatttatgt ttcattatta aaatacagc
//
LOCUS       DANLUA010000361          453 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.368, whole genome shotgun sequence.
ACCESSION   DANLUA010000361 DANLUA010000000
VERSION     DANLUA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.368"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(77..316)
                     /locus_tag="PXB44_002675"
                     /pseudo
     CDS             complement(77..316)
                     /locus_tag="PXB44_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT          136 a           95 c           70 g          152 t
ORIGIN      
        1 tccgcactca tattgccgtt accaacacct gctacgacaa taccttgata gcctacatct
       61 aacaaggctt tagtgggcca attttcacaa tctgctcacc tttgatattc gccaatgttt
      121 tcatttctgg cactgcttca attaaggttt cgatattcaa ttgccctgct tgataagcac
      181 tactatttgc attctgctga ctaccagcaa tcgttccgcc tgtcgctaaa atagtgatat
      241 taggtaattc tgccgcatta gctgttacaa ataagccacc taacattgct gctaatgcta
      301 attttttgaa tttcatataa ggctccttaa ttaaaaaaac taaagacaga taataccgat
      361 aaatcaatgt aaattatttg atctgataca taaaagcggg aattaattac tgggtagaaa
      421 taggttatat cgctattttc atttgggtta tct
//
LOCUS       DANLUA010000362          439 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.369, whole genome shotgun sequence.
ACCESSION   DANLUA010000362 DANLUA010000000
VERSION     DANLUA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.369"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..402)
                     /locus_tag="PXB44_002676"
     CDS             complement(<1..402)
                     /locus_tag="PXB44_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5283086.1"
                     /translation="MALAADLGYQTQVETLIKSLCYHHSHLKIYLFQKTFPNEWFDIW
                     CQKLALLSSEIIPVKVCVDFSKYKSLEHINETGFYRYLIPYLDEERVLYLDSDIVVDD
                     NLSEMYWLNFNGIPLYAVEDYVHNHIPAYLDL"
BASE COUNT          164 a           67 c           66 g          142 t
ORIGIN      
        1 taaatcaaga taagccggta tatgattatg aacataatcc tctactgcat ataaaggtat
       61 accattaaaa ttaagccagt acatttctga taaattatca tcaaccacaa tatctgaatc
      121 taaatatagt acacgttctt cgtccagata tggaataagg taacgataaa aacccgtttc
      181 attaatatgc tctaaactct tatattttga aaaatctaca cagactttca caggaataat
      241 ttcactcgat aataaggcta atttttggca ccagatatca aaccattcat taggaaaagt
      301 tttctgaaat agataaatct taagatgaga atgatgataa caaagcgatt taattaaggt
      361 ttcaacttga gtttgatagc ctaaatcagc tgctaatgca ataactattg gttggttggt
      421 tgattgattg attgattga
//
LOCUS       DANLUA010000363          431 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.370, whole genome shotgun sequence.
ACCESSION   DANLUA010000363 DANLUA010000000
VERSION     DANLUA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.370"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>431
                     /locus_tag="PXB44_002677"
     CDS             <1..>431
                     /locus_tag="PXB44_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5283087.1"
                     /translation="IFISAGGVRSIWLRHNLANFKQRLNALEKAVAEKGIILSENQVQ
                     ALERKKEDDVACGEIETAHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLY
                     TMKTAISAADMLNDKVLPFFESQGLPMLRILTDRGSEYCGKV"
BASE COUNT          126 a           78 c          105 g          122 t
ORIGIN      
        1 atttttattt cagcaggtgg tgtgcgttct atttggctac gccataatct cgctaacttt
       61 aaacagcgtt taaatgcgtt agaaaaagca gtggctgaga aaggcattat tctgagtgaa
      121 aaccaagttc aagccttgga gcgtaaaaaa gaagacgatg tggcttgtgg cgaaattgaa
      181 acggctcatc cgggctatct aggttcacaa gataccttct atgtggggaa tcttaagggg
      241 gttggacgca tttatcaaca gacctttatt gatacttata gtaaagtcgc atttgcaaaa
      301 ctgtatacga tgaagacagc aattagtgca gcagatatgc tcaatgataa agtcctaccg
      361 ttctttgagt cccaaggatt accgatgttg cgtattctta ccgaccgagg cagtgagtac
      421 tgtggcaagg t
//
LOCUS       DANLUA010000364          419 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.371, whole genome shotgun sequence.
ACCESSION   DANLUA010000364 DANLUA010000000
VERSION     DANLUA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.371"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            44..>419
                     /locus_tag="PXB44_002678"
     CDS             44..>419
                     /locus_tag="PXB44_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5283088.1"
                     /translation="MLSALIGLSQVSYANPLPLGNTTGTNNLAVGEGSFALGTGSQAI
                     GKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQIAEQEKQGSVNQQLQDALKSQI
                     AQYDTILERVREKQAQQAILANS"
BASE COUNT          139 a           91 c           80 g          109 t
ORIGIN      
        1 ccatctgtat aaaaaacaca caaaagagct ccctactgtt accttgctta gcgctctgat
       61 agggcttagc caagtctctt atgccaatcc acttccttta ggcaacacca ctggcaccaa
      121 taaccttgct gtaggtgaag gtagttttgc gttaggcaca ggttctcaag caatcggcaa
      181 gaatgccatt gcaaccggtg gtaatatctc cccccaggaa ttcaatgtgg agttagaaaa
      241 cttccgtgat ttagttaatc aaattgaaga gttacgtact caaattgctg aacaagaaaa
      301 acaaggctct gttaatcaac aattacaaga tgctttaaag agtcagattg ctcaatatga
      361 cactatttta gagcgtgtac gtgaaaaaca agcacaacaa gcaatattag ccaatagtg
//
LOCUS       DANLUA010000365          408 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.372, whole genome shotgun sequence.
ACCESSION   DANLUA010000365 DANLUA010000000
VERSION     DANLUA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.372"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            28..>408
                     /locus_tag="PXB44_002679"
     CDS             28..>408
                     /locus_tag="PXB44_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5283089.1"
                     /translation="MHSILKKILGKSKSSSQSSVKSDNVFSIEQDSEGWLYPKNASQL
                     LNTELRQKYLGLLWQQVSMNRQMFEALYQQPIERYAEMVQLLPASESHHHSHIGGMLD
                     HGLEVIAFSAKVRQNYILPPNVAPE"
BASE COUNT          123 a           92 c           74 g          119 t
ORIGIN      
        1 aaacatcatc tttttcggtc tatttcaatg cactcaattc tcaaaaaaat tcttggcaaa
       61 agtaaatcct cgtctcaaag ttcggttaaa tctgacaatg ttttctctat tgagcaggat
      121 agtgagggct ggttatatcc gaaaaatgcc tcccaactac ttaatacgga attacgccaa
      181 aaatatctgg gtctactttg gcaacaggta tcaatgaacc gtcaaatgtt tgaggcactt
      241 tatcaacagc caatagagcg ttatgccgaa atggtgcaac tgttacccgc ttccgagtca
      301 catcatcatt cacatatcgg cggaatgctc gatcacggtt tggaagttat tgctttttca
      361 gccaaagtcc gtcaaaacta tatattgcca ccgaatgtcg cacctgaa
//
LOCUS       DANLUA010000366          395 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.373, whole genome shotgun sequence.
ACCESSION   DANLUA010000366 DANLUA010000000
VERSION     DANLUA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.373"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          129 a           58 c           75 g          133 t
ORIGIN      
        1 agggattaca ctccactata aaggggtatc aagttcggta catgctggct ttacaaaagg
       61 aaatacatta caagaacaaa aattcatttc actcaaagtt ggatatgagt ggtaagcttt
      121 atgtaacctg aagctacact tgaatcggtt caaacataat gttactaaaa aagccttgaa
      181 acattgattt tcaaggcttt ttaggtatcg gttgaaccgt ataggattat aatttggtgc
      241 tggggtcatc tgaattgtat cacttcaagc attgataaat aagggttttg gttaattcaa
      301 attttatttg tactcctaaa tatactccta aaattcaaag tgccttattg cttaggctat
      361 tttgtgtaca attattcgga tcgtgtatag ggaaa
//
LOCUS       DANLUA010000367          394 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.374, whole genome shotgun sequence.
ACCESSION   DANLUA010000367 DANLUA010000000
VERSION     DANLUA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.374"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          135 a           73 c           54 g          132 t
ORIGIN      
        1 aatatctgtt tataaaataa cctaattaat aaggcacttt ggattttagt tatatcctga
       61 aaaatcaaat tataaagaaa ccttatctat caatacttga agccattaag ttcaagtctc
      121 gaccaggcac catttgacta aatttagttc aacagagcta aacgaaaaaa ccttagaaag
      181 cctgtaaaat caacgtttac aggctttttt gttgttttag ctcaatggca ctaaacattt
      241 catatcgcaa attttaagta gcctagcaaa ttttccgcca aatatgaccg cttgtaatgc
      301 cgttaggtta ttgcctggcg gcctttttgt tacagctctt atatttttta caccaaagtt
      361 cagaaaaagt cacttttatc actaacatta agat
//
LOCUS       DANLUA010000368          388 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.375, whole genome shotgun sequence.
ACCESSION   DANLUA010000368 DANLUA010000000
VERSION     DANLUA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.375"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          148 a           65 c           56 g          119 t
ORIGIN      
        1 aaaatagaca gataaaaata tgacaccctt aattaaaaaa tctgaaatca aataattcac
       61 aattgctgat agtgccataa aaatcacttt aaacatttat cctcctaagc acttaataca
      121 taacgtaaag caataaacgc ccaaataatg ccgaataata gctttaagat ttctttatgt
      181 ggttctatca aatcgcaatg ttctctaagt tcataatgaa atctcaagta ttcaatatta
      241 aatgtccaaa cagggcattg tgcggttcgt ttattcagtt caaaattctg taaatctggt
      301 agcaattttt taaaagggga tataattgat tccgccgtag gaacttccag atcaggcaaa
      361 ttaatatcag gtgtagaaga tcagactt
//
LOCUS       DANLUA010000369          381 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.376, whole genome shotgun sequence.
ACCESSION   DANLUA010000369 DANLUA010000000
VERSION     DANLUA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.376"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>381
                     /locus_tag="PXB44_002680"
     CDS             <1..>381
                     /locus_tag="PXB44_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5283090.1"
                     /translation="SANKLRRFIANKNNFLFKLISFGSNQLFKNKSTYTSIAIFKKSL
                     DNNILLYNNIRDYKKWFDNSALDYGEDKWHVYNLPLPDDPWYFKNTQDTLVFSHLRKI
                     CSPLEKIIGGKSISNGIQTSANKE"
BASE COUNT          153 a           53 c           51 g          124 t
ORIGIN      
        1 ctcagccaac aagctaagaa gatttatagc caataaaaac aactttttat ttaaattgat
       61 ttcctttggg agcaatcaat tatttaaaaa caaatcaaca tatacttcta ttgctatttt
      121 taaaaagagt ttagataaca acatattatt gtataacaat atacgagact ataaaaaatg
      181 gtttgataat tcagctcttg attacggaga agataagtgg catgtatata atttgccatt
      241 accagatgat ccttggtatt ttaaaaatac ccaagatact ctagtatttt ctcatttaag
      301 aaaaatttgt tctccattag aaaaaataat tggtggcaaa tctattagca atggaataca
      361 aaccagtgca aataaagagt a
//
LOCUS       DANLUA010000370          380 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.377, whole genome shotgun sequence.
ACCESSION   DANLUA010000370 DANLUA010000000
VERSION     DANLUA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.377"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..300)
                     /locus_tag="PXB44_002681"
     CDS             complement(<1..300)
                     /locus_tag="PXB44_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5283091.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVG"
BASE COUNT          130 a           67 c           67 g          116 t
ORIGIN      
        1 accaactttg cctaaaaaag atggtttcct tttcttttcc caaattaagt tagaaatgaa
       61 attcttccgc ccaaaaattt catcaagcat aattttcaaa tagtgacttt cgttatcatc
      121 aagagtgatc caaattgaac catcttcggc aagtaactct cgcagaattt caaggcgtgg
      181 atacatcatt gaaagccaaa tggaatgctc taaattatca tcgtagtggg taaaggccga
      241 ttttgtgtta tagggaggat caataaaaat acatttgact tgccccgcat aaaagggcaa
      301 aagagatttt aaggctgata aattatcacc ctgaataatt aaattttgct tgactttaga
      361 taaggtttca gcagaagctg
//
LOCUS       DANLUA010000371          372 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.378, whole genome shotgun sequence.
ACCESSION   DANLUA010000371 DANLUA010000000
VERSION     DANLUA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.378"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          134 a           64 c           51 g          123 t
ORIGIN      
        1 acatcgcatt gttcaaaaaa ttgaacaatt atttgcagag attgaaaaat tgtaggagtc
       61 atcatcgctt tcttgggcgt gttggatacg ccctacagtc actaaattat tgatctttta
      121 tcatatcggt tttcaaccaa cctaaaagga aacctatatg atccatctat ttgaaaagca
      181 ccttaatcaa tctaatcttg ctgaaaatac ccaatcagct tatctttttg cggtaaaaca
      241 atatcacaaa aaatttgatc agttatctaa aatcaattta cagcgatata aggtattctt
      301 aattgaaaac tataagcctc agactgttaa tttaaggcta agagccataa attattatct
      361 ggaatttatt aa
//
LOCUS       DANLUA010000372          353 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.379, whole genome shotgun sequence.
ACCESSION   DANLUA010000372 DANLUA010000000
VERSION     DANLUA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.379"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..291
                     /locus_tag="PXB44_002682"
     CDS             <1..291
                     /locus_tag="PXB44_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5283092.1"
                     /translation="DIKIIDELGDDKAVIAQKEIQAEIALCKRIFKQLDWSAVDFRIV
                     KRLYRKGFSENEIAVALVRFTDFEDRHCDSHDYLTRTITKAIQNYQQCSKAC"
BASE COUNT          138 a           45 c           60 g          110 t
ORIGIN      
        1 gacatcaaaa ttattgatga gcttggagat gataaagcag ttattgcaca aaaagaaatt
       61 caagcagaaa ttgctttgtg taaacgtata ttcaaacaat tagattggtc tgctgttgat
      121 tttagaattg ttaagcgtct gtacaggaaa ggattttcag aaaatgaaat tgctgttgca
      181 cttgtcagat ttactgattt tgaagacaga cattgtgatt cacatgatta tttgacaaga
      241 acaatcacaa aagccattca aaactatcaa caatgctcaa aagcttgtta gatgttataa
      301 ggaaagttaa aaatcaacaa aagatttgac aaatttatat aaaatcctta tta
//
LOCUS       DANLUA010000373          350 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.380, whole genome shotgun sequence.
ACCESSION   DANLUA010000373 DANLUA010000000
VERSION     DANLUA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.380"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>350
                     /locus_tag="PXB44_002683"
     CDS             <1..>350
                     /locus_tag="PXB44_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283093.1"
                     /translation="ISNHLITLAIYDENIQIQYSPQFIAKNLTMSDIQALQKLGFQFS
                     HSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQPIIAKAKSYQKSIEFSFDDTISVI
                     AAPITIPASIILFV"
BASE COUNT          132 a           61 c           52 g          105 t
ORIGIN      
        1 agatcagtaa tcacctaatt actttagcca tttatgatga aaatattcaa attcagtaca
       61 gtccgcaatt tattgcaaaa aatctcacca tgagcgatat tcaagctcta caaaagttag
      121 gttttcaatt tagccattca aaagaatatc tatggaaaag ctatgcaggt cttggtggta
      181 aaatcacaca gtttaaaaat cgcaaggaaa tactagaaaa gtataaattt aagcaaccta
      241 taatcgctaa agcgaaaagt tatcagaaaa gcatagaatt tagctttgat gacaccattt
      301 cggtcattgc agcaccaatt acgattcccg catctattat tttatttgta
//
LOCUS       DANLUA010000374          338 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.381, whole genome shotgun sequence.
ACCESSION   DANLUA010000374 DANLUA010000000
VERSION     DANLUA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.381"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          104 a           79 c           76 g           79 t
ORIGIN      
        1 ctgccgttga ttcactttct atcggtgcaa ggaacttact gatagacagt acttacaacc
       61 aacacatcaa ctacaataca aaaatgtcat caatcacacg cacagtctat cgcggcaata
      121 tgttgatacg actcggaatg cttggtggcg caggtgtggc cggtattgta caagcaccag
      181 ccgctcaagt aagtaatatc agacagggtc aagagtacac acttagcctg aatgtacagg
      241 gtacagccgg catacaaaaa acggggctca actatgtatt cttgatgcgt gctgatgggg
      301 ctaatcaacg gttagcaaca atacctgtta tagccagc
//
LOCUS       DANLUA010000375          338 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.382, whole genome shotgun sequence.
ACCESSION   DANLUA010000375 DANLUA010000000
VERSION     DANLUA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.382"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            124..>338
                     /gene="raiA"
                     /locus_tag="PXB44_002684"
     CDS             124..>338
                     /gene="raiA"
                     /locus_tag="PXB44_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL5283094.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLYT"
BASE COUNT          122 a           59 c           53 g          104 t
ORIGIN      
        1 gttaagtaga tcaaattttt ggaaacttat ttcatttttc acttgaattt ttggagtgat
       61 gagcatattt tggttattag aatatcaacg gcaaaagcct taactaaaca agaggaaaat
      121 gttatgtcat taaatatttc aagcaaacaa atggacatta cccctgcaat ccgtactcat
      181 attgaggagc gtttagctaa gttagaaaaa taccacaccc aattgataaa cccacatttt
      241 atgctgataa aacaacctaa ctcattccaa gttgaagcaa caattggcac ccctttaggt
      301 gatttagtcg ctaaagctga acatgaagat ttatatac
//
LOCUS       DANLUA010000376          335 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.383, whole genome shotgun sequence.
ACCESSION   DANLUA010000376 DANLUA010000000
VERSION     DANLUA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.383"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>335
                     /locus_tag="PXB44_002685"
     CDS             <1..>335
                     /locus_tag="PXB44_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5283095.1"
                     /translation="AGQERCIADEVPFEIPQNWTWVRLGEVGDWSSGATPNRHNPEYY
                     NGTIPWLKTGDLNNGIISSIPEFITDKALQECSVRLNPVGSVLIAMYGATIGKLGILK
                     IAATTNQAC"
BASE COUNT          108 a           55 c           83 g           89 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggact
       61 tgggtgaggt tgggggaagt aggtgattgg agttcaggtg caactccaaa ccgacacaat
      121 cctgaatact ataatggtac gatcccgtgg ctaaaaacag gagacttaaa caacggaata
      181 atttcatcta tacctgaatt tattacagat aaagcattgc aggaatgttc tgtaagatta
      241 aatcctgttg gttcagtttt gatagcaatg tatggagcga caattggtaa gcttgggata
      301 ttaaaaatag cagcaacaac aaatcaggct tgctg
//
LOCUS       DANLUA010000377          325 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.384, whole genome shotgun sequence.
ACCESSION   DANLUA010000377 DANLUA010000000
VERSION     DANLUA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.384"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>325)
                     /locus_tag="PXB44_002686"
     CDS             complement(<1..>325)
                     /locus_tag="PXB44_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5283096.1"
                     /translation="EYIARMDADDISLPTRLSEQILYLEENHLDLCGCNALLFSEHGN
                     LGLTNKLNSHEALEKLMVAGDIGIIHPTFLARRKVFVESGGYNPLALHAEDMEFIAHI
                     LSMGF"
BASE COUNT          115 a           69 c           42 g           99 t
ORIGIN      
        1 ctaaatccca ttgataaaat atgggcaata aattccatat cttccgcatg taaagctaag
       61 ggattatacc ctccagattc aacaaatact tttctccttg ctaaaaaagt agggtgaatt
      121 attccaatat caccagccac cataagcttt tctaaagcct catgactatt caacttattt
      181 gtaagaccaa ggttgccatg ttcagaaaaa agcaaagcat tacaaccgca taagtctaaa
      241 tggttttctt ctaaatataa aatttgctct gacaatctag taggtaaaga tatatcatca
      301 gcatccattc tcgctatata ttcac
//
LOCUS       DANLUA010000378          313 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.denovo.385, whole genome shotgun sequence.
ACCESSION   DANLUA010000378 DANLUA010000000
VERSION     DANLUA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.denovo.385"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            15..>313
                     /locus_tag="PXB44_002687"
     CDS             15..>313
                     /locus_tag="PXB44_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283097.1"
                     /translation="MSKLNKLLSRKTSRKIIPEQVNFDINLPIYIPLFLSDKTAKIQA
                     VWELEQENGERDAGAVKRNRIQFRYLPEGVHKLTLIVGTPLLSKGSKVYQCLIKV"
BASE COUNT          113 a           46 c           60 g           94 t
ORIGIN      
        1 caaaggaaca ttctatgtct aagttaaaca aattattatc gagaaaaaca tcgcgtaaaa
       61 ttattcctga gcaagttaat tttgacatta acttgcctat ctatatcccg ctgtttttat
      121 cagataagac agcgaaaatt caagcagtat gggagttaga acaagaaaat ggtgagcgtg
      181 atgctggcgc tgtaaagcga aatcgtattc agtttagata cttgccagaa ggggtacata
      241 aattaacatt aattgttggt acgcctttat tatcaaaagg aagtaaggtt tatcaatgcc
      301 taattaaagt taa
//
LOCUS       DANLUA010000379         7859 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.guided.116, whole genome shotgun sequence.
ACCESSION   DANLUA010000379 DANLUA010000000
VERSION     DANLUA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.guided.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..218)
                     /locus_tag="PXB44_002688"
     CDS             complement(<1..218)
                     /locus_tag="PXB44_002688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283098.1"
                     /translation="MPENSDWISCSGCENSRSSGKKPNFDPKPQKTRNDSSYNLFIWG
                     RAPVALATLNDSSYNSDEPATPAQRRAS"
     gene            complement(381..1673)
                     /locus_tag="PXB44_002689"
     CDS             complement(381..1673)
                     /locus_tag="PXB44_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL5283099.1"
                     /translation="MGTYIPHREGIIQTIVRNSLTQYQAYASPSQTILDRLEHFAECA
                     DWTKGVVRIRCHDCGHSYFRPFSCKVFHLCPSCDQKRTLLYAEYLAEDLLLDLPHRQF
                     VFTIPKILRPYFKSDKRLFGEVSRLVFSLLSDFFSLAAGQELLCACVVSYQSFGEFAR
                     FHPHWHVLVLEGGFTKYDRFVYLPIGADEGMLKVWQAAIMALFLRKELIDQARVNMLR
                     DWKHSGFSIESETRLFNKADREALGQYVVRGATCAEKIQYDPASDTIIWTASPKGFYK
                     GKTETFKGFEFVDQLVAHLPPRRVQLLRRYGVYAGKVRKQWQERPNIYSLAPESWQKG
                     HPRESKIVPAIPQEKPETVQVSDAWSKLRKQSWARLLQKVYEVDPFVCPKCQGTMSVV
                     AIIEDPKELAKIINWAKQQEREQPVAVCARSPPELALV"
     gene            1836..2681
                     /gene="sul2"
                     /locus_tag="PXB44_002690"
     CDS             1836..2681
                     /gene="sul2"
                     /locus_tag="PXB44_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDL5283100.1"
                     /translation="MEYTSKSQIARNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQA
                     RKLMAEGADVIDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQ
                     AYALSRGVAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHI
                     AAFFDARIAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLL
                     SVSRKSFLRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKE
                     TARIR"
     gene            2850..3653
                     /locus_tag="PXB44_002691"
                     /note="aph(3'')-Ib"
     CDS             2850..3653
                     /locus_tag="PXB44_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDL5283101.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3653..4489
                     /locus_tag="PXB44_002692"
     CDS             3653..4489
                     /locus_tag="PXB44_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDL5283102.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            4825..5640
                     /locus_tag="PXB44_002693"
     CDS             4825..5640
                     /locus_tag="PXB44_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDL5283103.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(5794..6387)
                     /locus_tag="PXB44_002694"
                     /pseudo
     CDS             complement(5794..6387)
                     /locus_tag="PXB44_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
     gene            complement(6388..6537)
                     /locus_tag="PXB44_002695"
                     /pseudo
     CDS             complement(6388..6537)
                     /locus_tag="PXB44_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            6557..7063
                     /locus_tag="PXB44_002696"
     CDS             6557..7063
                     /locus_tag="PXB44_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283104.1"
                     /translation="MAYQTLKSTQSQTQEKSDERAKQLLGSRVVEQVSESERENGNGR
                     EISSGSRQTSNEQRTGYSGNSSQEYRQISKSGERLTNKTSENALIEPTRPIKNKNGSK
                     VAPTPTNTPKTQNNANSNLLSSEQYRQTMEFLREFNDRLEQIAQNILENQLKALRTKA
                     KPSHCQWY"
     gene            7041..7613
                     /locus_tag="PXB44_002697"
                     /pseudo
     CDS             7041..7613
                     /locus_tag="PXB44_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
     gene            7626..>7859
                     /locus_tag="PXB44_002698"
     CDS             7626..>7859
                     /locus_tag="PXB44_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5283105.1"
                     /translation="MKALYCIMMSALLITGCTTSTEELLPANGSTMRDIWDKGAGNND
                     LHLYRSQQGRTLDPVDYIPNTEQRAYTRTAENEI"
BASE COUNT         1948 a         1966 c         2018 g         1927 t
ORIGIN      
        1 atcgacgctc gccgttgtgc tggtgttgcc ggttcatcag aattatagga actgtcattt
       61 aaagtcgcta aggctacagg tgcacgcccc caaataaata aattatagga actgtcattt
      121 cgggtttttt gcggtttcgg gtcaaaattc ggttttttcc ccgaactacg actgttttcg
      181 caccccgaac aactaatcca atcagaattt tccggcaacg ccgactccaa gaagtgtaat
      241 tccagtctat ttctgccgtc aacccctgat tcgcgttaat gcgacaaagc tcaaacacat
      301 aactccccca cgaagccata taaactccga tttcgaggtg ttatcctgtt ccgcataaac
      361 ttcgtttttg ggtattatac ctacaccaaa gcaagctcag gaggtgaacg agcacagacg
      421 gccactggct gctcccgttc ctgctgcttt gcccagttaa taatcttggc aagctccttg
      481 ggatcctcga ttatcgccac aaccgacatt gttccctggc attttgggca aacaaacggg
      541 tcaacttcat agactttctg taacaaccgt gcccaacttt gtttgcgcaa cttggaccag
      601 gcatcggaaa cttggacagt ttccggtttc tcctgaggta tggctggaac aattttggat
      661 tcgcgtggat gacctttttg ccaactttct ggggccaggc tataaatatt ggggcgctct
      721 tgccactgct tgcggacttt gcccgcatat acgccatagc gacgaagcaa ttgaacccgc
      781 cttggtggta aatgggccac aagctggtcg acgaattcaa agcctttgaa ggtctctgtt
      841 tttcctttgt agaatccctt tgggctagct gtccagatta tcgtgtcgga agctggatca
      901 tactgaattt tttctgcaca ggtagcgccg cgaacgacat actggccgag ggcttctcga
      961 tcggccttgt tgaatagtct ggtttcgctc tcgatgctaa agccggaatg cttccagtct
     1021 ctgagcatgt tcactcgggc ttgatctatt agttcctttc gcagaaacaa agccataatc
     1081 gctgcttgcc agaccttgag catcccttcg tcggctccaa ttggtaaata tacaaagcgg
     1141 tcgtacttgg tgaacccacc ttccagaacc agaacatgcc agtgcgggtg gaatcgagcg
     1201 aattcaccaa acgactgata gctgacaaca caggcacata gtagttcttg accggctgcc
     1261 agggagaaaa aatcagaaag caaggagaat accagcctgg acacctcgcc aaacagtcgc
     1321 ttatccgatt taaagtatgg tctgagaatc ttgggaatcg taaaaacaaa ttgccgatgc
     1381 ggtagatcaa ggagcaaatc ctcggccagg tattcagcat agagtaaggt ccgtttttgg
     1441 tcacaggatg ggcaaaggtg gaaaaccttg cacgaaaatg gcctgaagta cgaatgtcca
     1501 caatcatggc aacgtattcg aacaacaccc ttggtccaat cggcacattc agcaaaatgc
     1561 tcaaggcggt caaggatagt ttgcgatgga cttgcgtagg cttggtactg ggttagagaa
     1621 ttgcggacta tggtctggat tatcccttcc cgatggggaa tgtaagttcc catacctata
     1681 catacgcagg aaaacgatca tttgcttgca attgcgaaga aaataatgga aaatgtggac
     1741 tggcgccacc taacaaccgg ttcgagcgga ctcacgggac aagcccgttc gcagctcaac
     1801 cgaaagttag gttgacgcct tcggcgcgga ggaaaatgga atatacaagt aaatcacaaa
     1861 tagctcgaaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct
     1921 ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg
     1981 ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac gccgcgcctg
     2041 tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg
     2101 gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg
     2161 gtgtggccta tctcaatgat atccgcggtt ttccagacgc tgcgttctat ccgcaattgg
     2221 cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag gcagatcggc
     2281 gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac gcgcgcatcg
     2341 cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc ggcatggggt
     2401 tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc
     2461 ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc
     2521 tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag cttgccgccg
     2581 ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg
     2641 cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt
     2701 ctctatattc gcggttcagc aggcatgtcc cctttgaggg cgacccgacg acaggataat
     2761 cgaccttatg gtgcgcaaat atttcggcac agatggtatt cgtggcaaag ccacctccgc
     2821 gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt tttggtgaat
     2881 cgcattctga ctggttgcct gtcagaggcg gagaatccgg tgattttgtt tttcgacgtg
     2941 gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag ctcgctggag
     3001 agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag gtgatcaact
     3061 ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga gtaccggcgg
     3121 ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag caacttggcg
     3181 ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg cgaatgttcg
     3241 gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta ccggacgagg
     3301 acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta ccggtgcggc
     3361 tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg ccgaacttca
     3421 tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag
     3481 cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc
     3541 cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg
     3601 accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat
     3661 gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt
     3721 ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt
     3781 gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa
     3841 tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc
     3901 cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat
     3961 tgcagcggaa ctgatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct
     4021 tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc
     4081 aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc
     4141 ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg
     4201 cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa
     4261 tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca
     4321 gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc
     4381 gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga
     4441 tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga
     4501 cttatcctat cccctgggaa cacatcaatc ttaccggaga atatcgttgg ccaaagcctt
     4561 agcgtaggat ttcgccctct cccgcaaacg acccctttat tgtaggtggg ccagttggtg
     4621 attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga
     4681 tccttcaact cagcaaaagt tcgatttatt caacaaatcc acgttgtgtc tcaaaatctc
     4741 ttatgttata ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     4801 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg
     4861 ccgcgattaa attccaacct ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     4921 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     4981 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     5041 aactggctga cggcatttat gcctcttccg accatcaagc attttatccg tactcctgat
     5101 gatgcatggt tactcaccac tgcgctcccc gggaaaacag cattccaggt attagaagaa
     5161 tatcctgatt caggtgaaaa tattgttgat gcgctggcag cgttcctgcg ccggttgcat
     5221 tcgattcctg ttagtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     5281 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     5341 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
     5401 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     5461 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     5521 tggaactgcc tcggtgaatt ttctccttca ttacagaaac ggctttttca aaaatatggt
     5581 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctga
     5641 tagttagtct ttgtagagac acaaacctga aattccggac accgcgcttc aggacatctc
     5701 cctggacgcc gatatctggc agtatccgga cggtactatc gaacgccggg ccaacggtac
     5761 tcccctgccc tttatgactg acgaccggct atttcaccca cgcacttctc catgaaattt
     5821 agcatcgtcg cggttaatgt cgaaaatttg ccacctgacg tggttggcat cgagggtgaa
     5881 aactgccgtg gcgagggtgt tctcctcatc cggatcgtcg gcagccagcc ggtaaattgg
     5941 cagttctgcg tcatgggtat ctgagagaat ggtttttgcc gccgcggcgt caaacggagg
     6001 ttgcgtttcc cgccactgca cgaggcgtaa ctgccgcgac gctgaactgt cggtaacgat
     6061 ctgctcaacg ccagccagtg ccgcatgcac cagatggttg gcatgcccgg ttaccgtcgt
     6121 caggggtaga acggaacagc cttgaccggt ggcctcaacg ctgaacagcc gcgcatcgcc
     6181 cagctgcccg agcgtatggt gaaacgcccc ggatcgcggc tggccggtaa gtatagtgat
     6241 ggcctcgtcg agggtgttcg cattcagcga ggcgcgagcc aggatctgac gcggtaatcc
     6301 ctgcggacga tacactgcac ggatgttatt caccgtgtta acgatacctt tttcatttac
     6361 cgcaaaggtg tggccgcaaa tcgacccctt gggtggcagt ccgaacagcg cccgatcatg
     6421 gtcgcgctct aggtagctat ccaacccatc gagcaggcgc tgttctggcc actcgccgct
     6481 ttcaaccttg ccgagtatcc acgcgcctac cagcaccttg cgccgggtgt cctccttggc
     6541 gtataacgag caagttttgg cttaccaaac cttaaaatcc acacaatcac agacacagga
     6601 aaaatcagat gaacgagcaa aacaactctt gggttcaaga gtggttgaac aagtcagcga
     6661 aagcgagcga gagaatggca acggacgaga aatttcgtca ggaagtcgcc aaacgagcaa
     6721 cgaacagcgg acaggatatt caggcaattc tagccaagaa tatcgccaaa tatcaaaatc
     6781 aggcgaaaga ttaactaata aaacgtctga aaacgctctc atagagccta caagacctat
     6841 caaaaataaa aatggtagta aggtagcacc cacaccaaca aacacgccta aaacgcaaaa
     6901 taacgcaaat tctaatctat tgtcatcaga acagtatcgg cagactatgg agtttttgcg
     6961 agaatttaac gaccgattgg agcaaatcgc ccaaaatatc ctagaaaatc agctaaaagc
     7021 attgagaact aaggctaaac catctcattg ccaatggtat tgaattactt gatgtagaag
     7081 gtgcgattgt ttcgggtaca gctactggcg actggactct atcttgtgta cgaggcgatg
     7141 taaaaagtat gacctttgtg ttcaatgatg ggcgtattgt cagcacgact aacaaaggga
     7201 atagtgagaa tatcggttgg ttatctgata gttcaggcat tccttgtgtt cccggggaga
     7261 gaaaaacaaa tgcaccagaa tatttaggca caaatttctt cttagcggct gccagtgccg
     7321 cagcccaagg gttttcacaa tcacaaacaa cgacagtagt tgatggcaat agtgttgttg
     7381 gagctgttac cggtgatcaa ggtaagttca tcttagggca agccatcggc ggtggtttaa
     7441 aagaatccgc cgactggttt agaaagcgtt ttgctgaaaa ctttgatgct gtctatgtac
     7501 cacctggaca tcgtgtcgct attcatatcg agaaagagat tgagatcgat tacaactcca
     7561 aaggacgtaa agtgaaatac ggaaacacat caactaatac acaacttgat taaaaggaac
     7621 aaacaatgaa agctttatat tgcataatga tgagtgcctt attgataaca ggttgcacaa
     7681 cttcaactga agaattatta cccgctaacg gcagtacgat gcgagatatt tgggataaag
     7741 gagcaggtaa taacgatcta cacttatacc gttcacaaca aggacgaaca ctcgatcctg
     7801 tcgattatat tcctaacact gagcaacgag cctatacccg tacggcagaa aatgaaatt
//
LOCUS       DANLUA010000380         5119 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.guided.171, whole genome shotgun sequence.
ACCESSION   DANLUA010000380 DANLUA010000000
VERSION     DANLUA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.guided.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..642
                     /locus_tag="PXB44_002699"
     CDS             <1..642
                     /locus_tag="PXB44_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL5283106.1"
                     /translation="AKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKETIE
                     GKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEGTA
                     TYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQISST
                     VHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            838..2247
                     /locus_tag="PXB44_002700"
     CDS             838..2247
                     /locus_tag="PXB44_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5283107.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            2393..2866
                     /gene="gpt"
                     /locus_tag="PXB44_002701"
     CDS             2393..2866
                     /gene="gpt"
                     /locus_tag="PXB44_002701"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5283108.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            2979..3602
                     /gene="gmk"
                     /locus_tag="PXB44_002702"
     CDS             2979..3602
                     /gene="gmk"
                     /locus_tag="PXB44_002702"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5283109.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            3692..5089
                     /locus_tag="PXB44_002703"
     CDS             3692..5089
                     /locus_tag="PXB44_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5283110.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
BASE COUNT         1564 a          972 c         1144 g         1439 t
ORIGIN      
        1 gcgaaagaga aagcggaagc ggagcgttta gcagcagaaa aagaagtaga tcctatagct
       61 attattaaag caaaaggtat tgatgataca aattacggct taaaacttgg taatagtaaa
      121 gaaactattg agggtaaagt cacagtcaga acacagagtt atttatataa tcagccatat
      181 tctgtagtaa cagcacaagc agttcaaaga aatggctggg aaaacggtaa ttatattgat
      241 aaagttgatg gaacagttac agttaaagga ttgaaaacag agaaattacc aacagagggt
      301 actgcaacat attcaggaaa agcatttaac cacgatggag ctaaaggttt taatggtggt
      361 gatctaacct ataatgttaa tttctctaag cgtaccggtt caggtagagt tgagggagaa
      421 tttggaaact taatcgtatt agaagagggg acgattaata atactcagat ttcttcaaca
      481 gtacatggca ataatccttt aaacctttct ggaaaatata cagtcgaatt tttcggtaaa
      541 aatgcagagg aaattgccgg tgaaattcag acaaaagcaa atcttactgg ggaagtgagt
      601 aattttggac ttgccggcac tcgtggcgat atccaaaaat aaccttttct aacgttcaat
      661 gccgtttaac gaaaaattaa acggcatttg tcttgatatt gcttgtagat accataagac
      721 acaatagttt agatttcaat ttacagcttc taataaccct ctcccacaag gggagagagt
      781 gataaatccc gtagaggcga acatataggt ccgcccctac caattttctt tatctttatg
      841 aaaaaatatc cccttttatt ttcgctgttc agccccgttg tatgggcaga aaaggtgatt
      901 gaacacaaca ctgacccgat ttcctttgag cgtcagccgc aagcggttaa atttgagcaa
      961 aatgttgtaa aaaattcaca aaaaccgacc gcttatcagc ctgcgtattc agtcaatcag
     1021 aatgttgaac agcaaattaa ccaagcgatt ctcagccaag attgggcaac gcttgaaccg
     1081 cttttaagcc gttatccgca aactgaaaat tttgacccgt tgttgtggca ctatgcgcaa
     1141 gccgcacttg cctatgcgaa aaaagatcat caaaccgcag tttttcacta tcgccaactg
     1201 ttagctaagc ccccgagttt ggtttaccct cgttttgatt tggcgttgat tttggcagaa
     1261 gatcagcaat ttcgtgcggc agagcaggaa tttaaggcta ttcaagccca tttaccgccg
     1321 gaacttgcta aaattgcccg caatactcaa gtccaaattg ctgaaaatga ggcgtggcag
     1381 cccgattttt atctgcaata tacccaaaca gataacgtga ataatgcctc atcttcgccg
     1441 attgtgaatg taaatgggcg gattttccgc aaaaatgcag acagtttgcc acaatcagca
     1501 aaaggcgtgc gttatggctt agggttgagt aaattgcaca acgtgaaagg caatcatttt
     1561 gtcggtttaa cgctcaatta tagcgggatt tactattggg ataaccgaga ttaccgtgag
     1621 caatccgtat ccgtttcgcc gaattatagc taccgcaccg tcgcttatcg gctgactttt
     1681 tcgcctttta ttgagcaaaa ttggctgggg agtagccgtt ataatgttca atttggggca
     1741 acactcaacg gtttgcgtta ccttaattcg caatggttgg tgtcggggag ttttacccat
     1801 ttgcaaaaac ggtattttga ggagctaacc gcaagccgtt ataacagcta tcagcaccaa
     1861 ctcagtggcg gtgtgcagtg gcaagcggtc aaaaatgggc ggttttttgc aaatttagac
     1921 ggcgtgcgtg aaattgcacg ggaaaaagca caaagctcga ataagtggct ggctcgagtg
     1981 ggggcggttt ttcaaggcga acgctgggca acgcaggcaa gtatcggctt tggcaagcga
     2041 tattttgccg acaagcacta tttgtttggc tataagcggt tagacacgga atatcaagcc
     2101 aatttagcgg tttggaaccg cacttggcat tggcagggca ttgtgccgaa gctgaatttc
     2161 cgttatcaaa aaattgacag caatattgct gatttttact ctcgccaaaa caaggaagtc
     2221 tttttcactt tagaaaagct attttagtgt tggttttttg tgctaagcat acgattgcgt
     2281 agctgccggc acaaaaaaat aaatgaaatg atggcgaaaa aagcattttc gggttatcct
     2341 agtgcaattt tttatatcgg ggtagccccc aattttatat taggttgttt ctatgagtga
     2401 aaaatacaca aatgaaaaat atgtcgtgac gtgggatatg ttccatatgc acgctcgcaa
     2461 gttggcagaa aatttattac ctgccacaca gtggaaaggg attattgcgg tgagccgtgg
     2521 cggcttgttt ccggcagccg ttttagcacg tgagttgggt attcgccacg tagaaacagt
     2581 gtgcattgcc agctacgatc acgatagcca aggtgagtta aatgtgttac acgcagccga
     2641 taccgatggc gaaggcttta ttgtggtaga tgatttagtg gataccggca acacggcaaa
     2701 agaaattcgc agaatgtacc cgaaagctcg ttttgtaacc gtgtttgcga aaccgcacgg
     2761 tgcaccgttg gttgatgatt atgtgattga tattccacaa gaaacgtgga ttgagcagcc
     2821 ttgggattta ggcattacct tcgttcagcc tttggcacgt agataatttt gtgtaatgga
     2881 caatgaaagt tgtccattat tttttattta ttcctatgaa atgtaggggc gaatcatcat
     2941 tcgccctatt cccaaaatca aataggtata tacaaataat gagtttaggc aatctttata
     3001 ttatttctgc cccaagtgga gcaggaaaat cttccttaat taacgccttg ttggcggatt
     3061 taccccgttc ggaagtacag ctttcgattt cacacactac ccgtcagcct cgtccggcag
     3121 aacaggacgg cgtgcattat tatttcactt cgcacgatga gtttaagcag ctgattgagc
     3181 aagggtattt tttagaatgg gcggaggtgt ttggcaatta ttacggcaca tcattgccga
     3241 tgattgaacg tagcctagag cagggcattg atgtcttttt ggatattgac tggcaagggg
     3301 ctagacaaat tcgggaaaaa gtgccgaatg tgaaaaccgt ttttattttg ccaccgtcaa
     3361 aaacagaatt ggaaaaacgt ttaatcgggc gtgggcaaga ttccgctgaa accattgcca
     3421 aacgtatggc agaggcggtg tcggaaatga accattacaa tgagttcgat tatgtgatta
     3481 ttaatgatga tttccaacag gcattggcag agctaaaagc gattttaatt gccgagcgtt
     3541 tgagcttagg ttcgcaacaa attcgtcatc agcatttgat tactgatttg ttaaataatt
     3601 aaatgaacag ttgttcattt aactggaaag gcaatctgtt tttgggtaga atacgcccat
     3661 tttcccctct ttattttcaa aaggaaatgt aatgaaactt tcaaaattag cattttctgt
     3721 ggcgttggct ctaaccgtaa cggcttgtac cacagggaat atgagcaatg acgggacttt
     3781 gcaacaagca cagcaaacct atcaaagcta tgatgaaatt accaaacaat atcaaattaa
     3841 cgagcaatgg tggcaaggct atacggatgc ggaattaaac cgtgtggttg atttagccat
     3901 caaaaataac cttgatttag ccaaaagcgg aattgcagtc aataaagcct tgtataacgc
     3961 taatttagta ggggcaaatt tagtgccaac ctttagtggt tcaggttctg tttctgcggc
     4021 taaaggttta ggttcggctt cttcaaataa tgtttcgacc ggtgtttcaa caaccgcagc
     4081 tcaagctgca tttaatttaa gttatactgt ggatttatgg cgacgtttag ccgataccgc
     4141 cagtgcggca gaatggacgc ataaagccac cattgaagac ttaaaagcta cccgtttatc
     4201 tattattaat gcggttgtga ctacttacta ccagttagct tactatcgtg atgcgattcg
     4261 tattaccgaa gagagcattc gccattatga gcaaatcagc cgtattttaa ctaacaaaat
     4321 gaatgccggt gcgattgata gtttagcggt tgatcaggct caacaagcgg tcttaaacgc
     4381 cagaaatagt gtaattgatc tgaaaaccaa ccaaaaaatt gccgagcaaa cattacgcaa
     4441 tttgttaaat gttcagccaa atactccgct taacattcgt tacccgaata ttatgagcgt
     4501 gaaattgcaa ggggtagatt taaatgtgcc agtgtcagcc attgctaatc gccctgatgt
     4561 agtaggctcg ttacaacgct tacaaagtgc atttaaaacc ttaaccgcaa ccgagaaaag
     4621 ctggttccca accttaacca tcggtggttc tattaccggt agtgcaacca atatcggcaa
     4681 tgtggcagat aaccctgtgg gtaacggctt aattaaattg gatttaccgt tcttagattg
     4741 gaatcgggtt aaaaataacg taaaaatttc tgaaacggat tatgtgactg cccgtttaaa
     4801 ctatgagcaa aaaattacct cagccttaaa tgaaattgac cgttattatt acagttatac
     4861 gcagtcgaga agctcgttaa aaatccttca agataccaat gcgaaaaata aacgtatcag
     4921 ttcatactat aaaaaccgtt atgaacaagg ggtgtcggag ttccgtgagt ggattaccgc
     4981 actgaatacc gagcttaatt ctcagttggc gattttaaat gcaaaatatg cgattttgac
     5041 caacgagaat gcggtttacc aagcaatggc aggtaaatac agaagataat tatatctaaa
     5101 actatatact atggttggt
//
LOCUS       DANLUA010000381         3425 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.guided.211, whole genome shotgun sequence.
ACCESSION   DANLUA010000381 DANLUA010000000
VERSION     DANLUA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.guided.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..213
                     /locus_tag="PXB44_002704"
     CDS             <1..213
                     /locus_tag="PXB44_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HDL5283111.1"
                     /translation="AATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQ
                     LGREVMGIVQGGSVSWQLGRQRGLER"
     gene            490..1704
                     /gene="floR"
                     /locus_tag="PXB44_002705"
     CDS             490..1704
                     /gene="floR"
                     /locus_tag="PXB44_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL5283112.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            1732..2037
                     /locus_tag="PXB44_002706"
     CDS             1732..2037
                     /locus_tag="PXB44_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5283113.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            2149..>3425
                     /locus_tag="PXB44_002707"
     CDS             2149..>3425
                     /locus_tag="PXB44_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL5283114.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRAS"
BASE COUNT          647 a          976 c         1043 g          759 t
ORIGIN      
        1 gccgccacgg acggcgagag cgtcagcggc aagtttaccg gaaccgtgca tctatcgagc
       61 ggcaagttcg ccgtggtcga gaaatcccat gagttcaccc ttgtcccgtg gcggccgatc
      121 atcgaccgcc aactcggccg cgaggttatg ggcatcgtgc agggcgggtc ggtgtcgtgg
      181 cagttagggc ggcagagggg gctggaacgc tgagtgcgcc catgccgcat tgcgaagcaa
      241 aagataatcg gataaaatgt agcaattcat attcgtaagc gtggagtaat cagatgggaa
      301 attccaagtc agcagacaag taagccgcaa caaccagtat tgttgttgcg gcgctctgta
      361 aggctagtct catctgattg ctgacgagca gacgtcgccc ggtattcctt aatcgagggt
      421 tgattcgtca tgaccaccac acgccccgcg tgggcctaaa caaatatcaa tcgcaaggag
      481 atcatattca tgaccaccac acgccccgcg tgggcctata cgctgccggc agcactgctg
      541 ctgatggctc ctttcgacat cctcgcttca ctggcgatgg atatttatct ccctgtcgtt
      601 ccagcgatgc ccggcatcct gaacacgacg cccgctatga tccaactcac gttgagcctc
      661 tatatggtga tgctcggcgt gggccaggtg atttttggtc cgctctcaga cagaatcggg
      721 cgacggccaa ttctacttgc gggcgcaacg gctttcgtca ttgcgtctct gggagcagct
      781 tggtcttcaa ctgcaccggc ctttgtcgct ttccgtctac ttcaagcagt gggcgcgtcg
      841 gccatgctgg tggcgacgtt cgcgacggtt cgcgacgttt atgccaaccg tcctgagggt
      901 gtcgtcatct acggcctttt cagttcggtg ctggcgttcg tgcctgcgct cggccctatc
      961 gccggagcat tgatcggcga gttcttggga tggcaggcga tattcattac tttggctata
     1021 ctggcgatgc tcgcactcct aaatgcgggt ttcaggtggc acgaaacccg ccctctggat
     1081 caagtcaaga cgcgccgatc tgtcttgccg atcttcgcga gtccggcttt ttgggtttac
     1141 actgtcggct ttagcgccgg tatgggcacc ttcttcgtct tcttctcgac ggctccccgt
     1201 gtgctcatag gccaagcgga atattccgag atcggattca gctttgcctt cgccactgtc
     1261 gcgcttgtaa tgatcgtgac aacccgtttc gcgaagtcct ttgtcgccag atggggcatc
     1321 gcaggatgcg tggcgcgtgg gatggcgttg cttgtttgcg gagcggtcct gttggggatc
     1381 ggcgaacttt acggctcgcc gtcattcctc accttcatcc taccgatgtg ggttgtcgcg
     1441 gtcggtattg tcttcacggt gtccgttacc gcgaacggcg ctttggcaga gttcgacgac
     1501 atcgcgggat cagcggtcgc gttctacttc tgcgttcaaa gcctgatagt cagcattgtc
     1561 gggacattgg cggtggcact tttaaacagt gacacagcgt ggcccgtgat ctgttacgcc
     1621 acggcgatgg cggtactggt ttcgttgggg ctggtgctcc ttcggctccg tggggctgcc
     1681 accgagaagt cgccagtcgt ctaaccgacg actggtagca ggcccgctcc gatgcggcgc
     1741 actaaccatc gaaacctcgt gaatgtcggt atcctgtctg gcaggatacc gctcatttcc
     1801 cttgttcagt tcatcgccgt cgccgagcat ctgaattttc ggcatgcggc caaggcactt
     1861 ggtatcagcc agtcgagcgt cagcgcgcgt gtgaaagcgc tggaggataa ccttggtgtc
     1921 ctgctatttg agcgccatgc gcggggcgtt cggctaacag acgcaggcag gcacttcatg
     1981 gagcgtgtca cggcgggtgt cgatcaactc gatcacgcag tgaagaccgc ggagtgacgg
     2041 gcactggctg gcaatgtcta gcaacggcag gcatttcggc tgagggtaaa agaactttcc
     2101 gctaagcgat agactgtatg taaacacagt attgcaagga cgcggaacat gcctcatgtg
     2161 gcggccagga cggccagccg ggatcgggat actggtcgtt accagagcca ccgacccgag
     2221 caaacccttc tctatcagat cgttgacgag tattacccgg cattcgctgc gcttatggca
     2281 gagcagggaa aggaattgcc gggctatgtg caacgggaat ttgaagaatt tctccaatgc
     2341 gggcggctgg agcatggctt tctacgggtt cgctgcgagt cttgccacgc cgagcacctg
     2401 gtcgctttca gctgtaagcg tcgcggtttc tgcccgagct gtggggcgcg gcggatggcc
     2461 gaaagtgccg ccttgctggt tgatgaagta ctgcctgaac aacccatgcg tcagtgggtg
     2521 ttgagcttcc cgtttcagct gcgtttcctg tttgccagcc ggcccgagat catggggtgg
     2581 gtgctgggca tcgtttaccg cgtcattgcc acgcacctgg tcaagaaagc gggccatacc
     2641 caccaagtgg ccaagacggg cgcggtcacc ctgatccagc gttttggatc ggcgctcaat
     2701 ctgaatgttc acttccacat gctgtttctc gacggtgtgt atgtcgagca atcccacggc
     2761 tcagcgcgtt tccgctgggt caaggcgccg accagcccag agctcaccca gctgacgcac
     2821 accatcgccc accgggtggg tcgctatctg gaacggcaag gcctgctgga acgggatgtc
     2881 gaaaacagct atctggcctc ggatgcggtg gatgacgacc cgatgacacc cctgctgggg
     2941 cactcgatca cttaccgtat cgctgtcggt tcacaggcgg ggcgaaaggt gttcactttg
     3001 caaactctgc cgaccagtgg tgatccgttc ggtgacggga ttggcaaggt agccgggtcc
     3061 agcctgcacg ccggcgtggc ggccagggcc gatgaacgca agaagctcga acggctgtgc
     3121 cggtacatca gccgcccggc ggtatccgag aagcggctgt cgttaacacg aggcggcaac
     3181 gtgcgctacc agctcaagac gccgtaccgg gacggcacca cgcacgtcat tttcgaacca
     3241 ttggatttca ttgcaaggct ggccgccctg gtaccgaagc ccagagtcaa cctaacccgc
     3301 ttccacgggg tgttcgcacc caacagtcgg caccgggcgt tggtcacgcc ggcaaaacgg
     3361 ggcaggggca acaaggtcag ggtggctgat gaaccggcaa caccagcaca acggcgagcg
     3421 tcgat
//
LOCUS       DANLUA010000382         3341 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.guided.218, whole genome shotgun sequence.
ACCESSION   DANLUA010000382 DANLUA010000000
VERSION     DANLUA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.guided.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            143..676
                     /gene="aadB"
                     /locus_tag="PXB44_002708"
     CDS             143..676
                     /gene="aadB"
                     /locus_tag="PXB44_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL5283115.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     gene            734..1525
                     /locus_tag="PXB44_002709"
     CDS             734..1525
                     /locus_tag="PXB44_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL5283116.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1602..2429
                     /locus_tag="PXB44_002710"
     CDS             1602..2429
                     /locus_tag="PXB44_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL5283117.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
     gene            2520..3224
                     /locus_tag="PXB44_002711"
     CDS             2520..3224
                     /locus_tag="PXB44_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDL5283118.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          817 a          815 c          929 g          780 t
ORIGIN      
        1 agatatatta cacccactct atcactgata gatttttagg atctcaatga ataaatcaat
       61 tattattata ctgctgatca ccgtattagc agcaacgatg ttacgcagca gggcagtcgc
      121 cctaaaacaa aggttaggcc gcatggacac aacgcaggtc acattgatac acaaaattct
      181 agctgcggca gatgagcgaa atctgccgct ctggatcggt gggggctggg cgatcgatgc
      241 acggctaggg cgtgtaacac gcaagcacga tgatattgat ctgacgtttc ccggcgagag
      301 gcgcggcgag ctcgaggcaa tagttgaaat gctcggcggg cgcgtcatgg aggagttgga
      361 ctatggattc ttagcggaga tcggggatga gttacttgac tgcgaacctg cttggtgggc
      421 agacgaagcg tatgaaatcg cggaggctcc gcagggctcg tgcccagagg cggctgaggg
      481 cgtcatcgcc gggcggccag tccgttgtaa cagctgggag gcgatcatct gggattactt
      541 ttactatgcc gatgaagtac caccagtgga ctggcctaca aagcacatag agtcctacag
      601 gctcgcatgc acctcactcg gggcggaaaa ggttgaggtc ttgcgtgccg ctttcaggtc
      661 gcgatatgcg gcctaacaat tcgtccaagc cgacgccgct tcgcggcgcg gcttaactca
      721 ggtgttagac atcatgaggg aagcggtgac catcgaaatt tcgaaccaac tatcagaggt
      781 gctaagcgtc attgagcgcc atctggaatc aacgttgctg gccgtgcatt tgtacggctc
      841 cgcagtggat ggcggcctga agccatacag cgatattgat ttgttggtta ctgtggccgt
      901 aaagcttgat gaaacgacgc ggcgagcatt gctcaatgat cttatggagg cttcggcttt
      961 ccctggcgag agcgagacgc tccgcgctat agaagtcacc cttgtcgtgc atgacgacat
     1021 catcccgtgg cgttatccgg ctaagcgcga gctgcaattt ggagaatggc agcgcaatga
     1081 cattcttgcg ggtatcttcg agccagccat gatcgacatt gatctggcta tcttgctgac
     1141 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     1201 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     1261 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1321 cgcaataacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa taaaacgcct
     1381 acctgcccag tatcagcccg tcttacttga agctagacag gcttatcttg gacaagaaga
     1441 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga
     1501 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc
     1561 ggcgcggctt accttggccg ttgggcatta aggaaaagtt aatggcaatc cgaatcttcg
     1621 cgatactttt ctccattttt tctcttgcca ctttcgcgca tgcgcaagaa ggcacgctag
     1681 aacgttctga ctggaggaag tttttcagcg aatttcaagc caaaggcacg atagttgtgg
     1741 cagacgaacg ccaagcggat cgtgccatgt tggtttttga tcctgtgcga tcgaagaaac
     1801 gctactcgcc tgcatcgaca ttcaagatac ctcatacact ttttgcactt gatgcaggcg
     1861 ctgttcgtga tgagttccag atttttcgat gggacggcgt taacaggggc tttgcaggcc
     1921 acaatcaaga ccaagatttg cgatcagcaa tgcggaattc tactgtttgg gtgtatgagc
     1981 tatttgcaaa ggaaattggt gatgacaaag ctcggcgcta tttgaagaaa atcgactatg
     2041 gcaacgccga tccttcgaca agtaatggcg attactggat agaaggcagc cttgcaatct
     2101 cggcgcagga gcaaattgca tttctcagga agctctatcg taacgagctg ccctttcggg
     2161 tagaacatca gcgcttggtc aaggatctca tgattgtgga agccggtcgc aactggatac
     2221 tgcgtgcaaa gacgggctgg gaaggccgta tgggttggtg ggtaggatgg gttgagtggc
     2281 cgactggctc cgtattcttc gcactgaata ttgatacgcc aaacagaatg gatgatcttt
     2341 tcaagaggga ggcaatcgtg cgggcaatcc ttcgctctat tgaagcgtta ccgcccaacc
     2401 cggcagtcaa ctcggacgct gcgcgataaa accgcgcagc gccggttact tcaacgggca
     2461 ctgttgcaaa tagtcggtgg tgataaactt atcatcccct tttgctgatg gagctgcaca
     2521 tgaacccatt caaaggccgg cattttcagc gtgacatcat tctgtgggcc gtacgctggt
     2581 actgcaaata cggcatcagt taccgtgagc tgcaggagat gctggctgaa cgcggagtga
     2641 atgtcgatca ctccacgatt taccgctggg ttcagcgtta tgcgcctgaa atggaaaaac
     2701 ggctgcgctg gtactggcgt aacccttccg atctttgccc gtggcacatg gatgaaacct
     2761 acgtgaaggt caatggccgc tgggcgtatc tgtaccgggc cgtcgacagc cggggccgca
     2821 ctgtcgattt ttatctctcc tcccgtcgta acagcaaagc tgcataccgg tttctgggta
     2881 aaatcctcaa caacgtgaag aagtggcaga tcccacgatt catcaacacg gataaagcgc
     2941 ccgcctatgg tcgcgcgctt gctctgctca aacgcgaagg ccggtgcccg tctgacgttg
     3001 aacaccgaca gattaagtac cggaacaacg tgattgaatg cgatcatggc aaactgaaac
     3061 ggataatcgg cgccacgctg ggatttaaat ccatgaagac ggcttacgcc accatcaaag
     3121 gtattgaggt gatgcgtgca ctacgcaaag gccaggcctc agcattttat tatggtgatc
     3181 ccctgggcga aatgcgcctg gtaagcagag tttttgaaat gtaaggcctt tgaataagac
     3241 aaaaggctgc ctcatcgcta actttgcaac agtgccgtta aagctcggtc aatcggcact
     3301 gattgagcct gactcaccac tgcaattagg atctttgatg c
//
LOCUS       DANLUA010000383         3233 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.guided.226, whole genome shotgun sequence.
ACCESSION   DANLUA010000383 DANLUA010000000
VERSION     DANLUA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.guided.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            946..1083
                     /locus_tag="PXB44_002712"
     CDS             946..1083
                     /locus_tag="PXB44_002712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5283119.1"
                     /translation="MKPLRIGARVIYPLQDLDKQIAYLEANLLENIKVHFIVESIEIK
                     G"
     gene            1218..2135
                     /locus_tag="PXB44_002713"
     CDS             1218..2135
                     /locus_tag="PXB44_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class A beta-lactamase
                     ROB-1"
                     /protein_id="HDL5283120.1"
                     /translation="MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQA
                     TFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKALLAGAVLQSLPE
                     KDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVE
                     QYQRILRQLGDNVTHTNRLEPDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQK
                     TILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKPIVMAIM
                     STQFTEEAKFNNKLVEDAAKQVFHTLQLN"
     gene            2146..3063
                     /locus_tag="PXB44_002714"
     CDS             2146..3063
                     /locus_tag="PXB44_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5283121.1"
                     /translation="MKKKLLWILILGLIIISCKQRKTEMKEKIIKTNGIELCTESFGN
                     KKNPAILLVAGATVSMLYWDTEFCQQLSEKGFFVIRYDNRDVGKSTNYEPGSTPYDIV
                     DLTNDAISILDGYKIDKAHFVGISLGGLISQIASIKFADRVNSLTLMSSGPWGDSDPT
                     IPEMDTSILDFHSKAGTVNWTNEDSVVNYLIQGAELMSGKKQFDKQRSEKLIRAEFNR
                     ANNYISMFNHAALQGGGEEYWNRLNEIKQPTLIIHGTDDKIWHYKNAGFLLEKIKGSN
                     LITLEGTGHELHVDDWKSIIDGIEKHIND"
BASE COUNT         1053 a          605 c          659 g          916 t
ORIGIN      
        1 tagggaagtg gttattgtgg gcggtaggtt tttagtgttg gaaagctgtt ggaaagctcg
       61 tgggtaactc gcagagttat ccatcagctt ttcaatggct tttcaatacg acccgcaggg
      121 gaaaaaccgt taaaccgtcc acattaaccg ctgacccaaa aagaacgctt tttactaaaa
      181 gatttctatt cggttctcaa atgttgcgtg aatggggcaa caataaaacg ttgtggcgtt
      241 tatcagggtg ggaattgcag acttaaacaa tcaattccgc tgtatcctta aatctcgctt
      301 atcaggtgtg cttgtatttc ttggggcttt cgcctttcta accgtagcac gaaacggaga
      361 cagagcttaa ttttcgtatt aagaacgtta tcacttctac gcctgtttat tcttccgaag
      421 tccgagctga agaccgtcta atttaaggtt tacgtttctt gtttacgtcg ttgaatggtt
      481 ttttggtagt agggacgctt cgcaatctct tgcattacta gctacccttt gctatgatta
      541 gattgttcac gtctggatca tagcaaagaa cgtactgata cagattatag gaaaagggat
      601 cgaatgtcaa aagttcgatc cctttttctt ttctaaagca ttggtattaa aggctttaag
      661 gtgtatttta ttaaaatctg tacacctttg tgaaaatcca tacacctttc ttaccgtgta
      721 attttatgaa aattcgtgca cctttctgct aacgccctgc ggttgttaga ctaaaaaagc
      781 cattgaaagc aagtttcaac ggctttttca gtcccgaata aaacaaaacc gccttgttag
      841 gcggttatgt gttaaaatca ctttcgcact atggcggcgg ctcgcactac cgttcatagt
      901 gtgagcctag tcgcttagcc ttatagtgct aagggggtga tttatgtgaa acctctacga
      961 attggggcaa gggttattta tccgcttcaa gacttagaca agcaaattgc atacctagag
     1021 gcgaacttac ttgagaatat caaagtgcat ttcattgtag aaagcattga gattaaaggc
     1081 taaattgcta gacttttgcg cttaaattcg ccaaaatctg tttttttgtt ctacaaacaa
     1141 attatccgaa tttccgcgca atgtacgttt caggctgcct gaaaagaaat ttatttagta
     1201 aaatcaaagg ataatttatg ttaaataagt taaaaatcgg cacattatta ttgctgacat
     1261 taacggcttg ttcgcccaat tctgttcatt cggtaacgtc taatccgcag cctgctagtg
     1321 cgcctgtgca acaatcagcc acacaagcca cctttcaaca gactttggcg aatttggaac
     1381 agcagtatca agcccgaatt ggcgtttatg tatgggatac agaaacggga cattctttgt
     1441 cttatcgtgc agatgaacgc tttgcttatg cgtccacttt caaggcgttg ttggctgggg
     1501 cggtgttgca atcgctgcct gaaaaagatt taaatcgtac catttcatat agccaaaaag
     1561 atttggttag ttattctccc gaaacccaaa aatacgttgg caaaggcatg acgattgccc
     1621 aattatgtga agcagccgtg cggtttagcg acaacagcgc gaccaatttg ctgctcaaag
     1681 aattgggtgg cgtggaacaa tatcaacgta ttttgcgaca attaggcgat aacgtaaccc
     1741 ataccaatcg gctagaaccc gatttaaatc aagccaaacc caacgatatt cgtgatacga
     1801 gtacacccaa acaaatggcg atgaatttaa atgcgtattt attgggcaac acattaaccg
     1861 aatcgcaaaa aacgattttg tggaattggt tggacaataa cgcaacaggc aatccattga
     1921 ttcgcgctgc tacgccaaca tcgtggaaag tgtacgataa aagcggggcg ggtaaatatg
     1981 gtgtacgcaa tgatattgcg gtggttcgca taccaaatcg caaaccgatt gtgatggcaa
     2041 tcatgagtac gcaatttacc gaagaagcca aattcaacaa taaattagta gaagatgcag
     2101 caaagcaagt atttcatact ttacagctca actaacaaat tcagcatgaa aaaaaaacta
     2161 ctttggatat taattttagg actgataata atcagttgca aacaaaggaa aacagaaatg
     2221 aaagagaaaa taattaaaac aaacggcatt gaactctgta cggaaagttt tggaaataag
     2281 aaaaatccag caatcctttt ggtagcaggt gcaaccgtat caatgctgta ttgggacact
     2341 gaattttgcc aacaattatc tgaaaaagga ttttttgtta ttcgttacga caacagagat
     2401 gtaggaaaat ccactaatta tgaaccaggt tctactccat acgatattgt tgacttaact
     2461 aatgacgcta tttcaatatt ggatggctac aagattgaca aagcacattt tgtggggatt
     2521 tctttgggcg gactaatttc tcaaatagca tcaataaagt ttgccgacag agttaactcc
     2581 ttaactctta tgtcatcagg cccttgggga gactcagacc caactatacc tgaaatggac
     2641 acgagtattt tagatttcca tagtaaagca ggtacagtca attggacaaa tgaagacagt
     2701 gtggtaaact atttaattca gggtgcagaa ttaatgagtg gcaagaaaca atttgacaaa
     2761 caaagaagtg aaaaactgat aagagctgag ttcaatagag ccaacaatta tataagtatg
     2821 ttcaatcacg ctgcattgca aggtggtggt gaagaatatt ggaacagatt aaacgaaatc
     2881 aaacaaccca ccttaattat ccacggaaca gacgacaaaa tttggcatta taagaatgca
     2941 ggttttttac tagaaaaaat aaaaggttca aatctaatca cccttgaagg tacaggacac
     3001 gaattacacg ttgatgattg gaaatcaata attgatggaa tagaaaaaca cataaatgac
     3061 tgataaacaa aaacaacgac ccgctaacaa tgtatataaa aaataggcga aacagtagta
     3121 aattcaaggc ttttggctcg tattatactt tgtgcttaac cgaaaatttg gcacttcgaa
     3181 atcgcctact tttcgctgaa tttacaatcc aagtgcaaca aaaaaagaag tac
//
LOCUS       DANLUA010000384         1354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995839-rid16719963.guided.286, whole genome shotgun sequence.
ACCESSION   DANLUA010000384 DANLUA010000000
VERSION     DANLUA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995839
            Sequence Read Archive: SRR18050807
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574664.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574664.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:13:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 35 of 72
            Pseudo Genes (incomplete)         :: 28 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 1 of 72
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995839-rid16719963.guided.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-03-12"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            47..1249
                     /gene="tet(H)"
                     /locus_tag="PXB44_002715"
     CDS             47..1249
                     /gene="tet(H)"
                     /locus_tag="PXB44_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5283122.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
BASE COUNT          358 a          275 c          275 g          446 t
ORIGIN      
        1 agatatatta cacccactct atcactgata gatttttagg atctcaatga ataaatcaat
       61 tattattata ctgctgatca ccgtattaga tgccattggt atcgggctta tcatgccagt
      121 actccctact ctattaaatg aatttgtcag tgaaaattca ctggcaaccc attacggtgt
      181 gctattagcg ctctatgcta ccatgcaggt tatttttgct cctattctag gacgactgtc
      241 tgataaatac ggcagaaaac ccatcttgct gttttccctt ttaggcgcgg cactcgacta
      301 tcttttaatg gcattctcaa ccacactttg gatgctctat attgggcgca tcattgcggg
      361 gatcacaggc gcaacaggtg ccgtatgtgc atcagcgatg agtgatgtga ctcccgctaa
      421 aaatcgaact cgctattttg gtttcttagg tggtgctttt ggtgttggcc ttattatcgg
      481 cccaatgcta gggggattat taggtgatat cagtgctcat atgccattta tttttgccgc
      541 tatttcacac tcgatattat taatactctc tttgctcttt ttccgagaaa cacaaaaaag
      601 agaagcgctt gttgccaata ggacacctga aaaccaaact gcctcaaata cagtcactgt
      661 tttttttaag aaaagcctct acttttggtt agcaacctat tttattatcc agcttatcgg
      721 gcaaattcct gccaccatct gggtgctgtt tacacaatat cgttttgatt ggaacacaac
      781 ttctatcggt atgtctttgg cggttctggg tgtattaaat attttctttc aggcgattgt
      841 cgctgggaaa ttggcacaaa aatggggcga aaaaaccacc attatgatca gtatgtctat
      901 tgatatgatg ggctgtttat tattagcgtg gataggccac gtttgggtca tcttaccagc
      961 attaatttgc ttagcggcag gaggtatggg gcaacccgca ttacaaggtt atttatcaaa
     1021 atctgtcgat gataatgcgc aagggaaatt acaaggtact ctggtgagcc taaccaatat
     1081 taccgggatc attggtcccc ttttatttgc ctttatttat agttatagcg tcgcttattg
     1141 ggatggtctg ttatggctga tgggggcaat actttatgct atgttgctta ttaccgctta
     1201 ttttcaccaa agaaaaacca cacctaaagc tgttatttca accccttaat actagaaatt
     1261 ataaagatca taaaagtcct gtccgctctt aataacgttg ataggacttt atttgggtat
     1321 ccggattaca gggttgaaac agggctataa acgc
//
